LOCUS       DASUYD010000001         2911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1322891, whole genome shotgun sequence.
ACCESSION   DASUYD010000001 DASUYD010000000
VERSION     DASUYD010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322891"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="VF195_00005"
     CDS             <1..453
                     /locus_tag="VF195_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6579250.1"
                     /translation="ELGIHLIQSDHNHIRRNRVRDNPEDGIILQGDHNQIVGNRVVRN
                     SITITLFTNNAHAVGNVVRGNTVRRAPRGGISIDQVIERTMVKRNHVFRAGGSGILVG
                     NPTTTLTRNEARYNHRLGIEAVEGVIDGGGNRASGNGDPRQCVNVVCS"
     gene            1457..2128
                     /locus_tag="VF195_00010"
     CDS             1457..2128
                     /locus_tag="VF195_00010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HEX6579251.1"
                     /translation="MAEAEVVDGDVIPAPPPAARRRHWGRILQITGLLFIAAALALGG
                     YLWWNLWGTGFAARAAQNDLRPAFERNVASLHPTAGPERVANVPGKAIAIIRIPRIEM
                     DLVVVEGTNTESLKKGPGHYSRTAYPWDDTGSVGIAGHRTTYGAPFWSLNELREGDRI
                     VLATEYGIFNYRVSRTAIVPPSGILPSGDYVLDQTKSPTLVLTTCNPRFSASERLVVF
                     ADRVG"
     gene            complement(2360..2533)
                     /locus_tag="VF195_00015"
     CDS             complement(2360..2533)
                     /locus_tag="VF195_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579252.1"
                     /translation="MDEALKRGVEVDDSSRNDRCAQRNWKTWLGVGLALAVVSVLVVL
                     IAANSGGGSGGLY"
     gene            complement(2716..>2911)
                     /locus_tag="VF195_00020"
     CDS             complement(2716..>2911)
                     /locus_tag="VF195_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6579253.1"
                     /translation="VVGDSVWDLLAARRARALGIGLLSGGYGLEELERAGAYRVYDDP
                     ADLRRHLDEVGVRQAGPAPE"
BASE COUNT          516 a          991 c          913 g          491 t
ORIGIN      
        1 gaactcggca tccacctgat ccaatccgat cacaatcaca tccggcgcaa ccgggtacgc
       61 gacaaccccg aggacgggat catcctgcaa ggcgaccaca accagatcgt cggcaaccgg
      121 gtggtgcgta actccatcac gatcacgctc ttcacgaaca acgcacatgc ggttggcaac
      181 gtcgtccgtg gcaacaccgt gcgtcgcgct ccccgcggcg ggatttcgat cgaccaagtg
      241 atcgagcgca ccatggtcaa gcgcaaccat gtcttccgag cgggaggcag cggtatcctc
      301 gtagggaatc ccacaacgac gctgacccgc aacgaggcca ggtacaacca ccgtctcggt
      361 atcgaagccg ttgagggtgt gatcgacggc ggcggcaacc gggcaagtgg caacggcgat
      421 ccgcgccaat gtgtaaacgt cgtatgtagc tgagccgacc ttcaacacga cagcagcgac
      481 gctcgcactc ctagcggtcg gtctcccgca gcatcccgac gagcaccgcc cgaagcgtcc
      541 tcgtcgcagt cgatcagctg tccgcgacgg tgcgggtctt gaagttccca gtaggagccg
      601 aatgtgtcga cccgctcgaa gtcggggagc ataaggacgt gcagcagttg ggcactgggg
      661 ccgaggatgt cgacgaggag cccgccgacc ggcgcgctcg gctcgaccca tatgacccct
      721 ccaccttccg cctcgcgggg ccagtgcgag cgcctttgca cgagcgatcc cgctctcctc
      781 cgggccatct tgaaggttcg ggatggcgca ggcggggcct actgatgggt ggaatgtggc
      841 acctacgatt tgcggctgtc cgcctccacc agcacacttc gtttgctcgg cccgtaggtg
      901 aggctcagtc cgaggccgtc gtagtgcatg gggcatcgag gaggcactgt gggtggcatc
      961 catggacccg cccggatggg ctgtgtgatc cttccctcaa cgcgagcgcc cgctcgggcg
     1021 ccgacgtgga tgtgctcgag acaggctatg cgatggctgg tgtgggtagc gcggaccacc
     1081 cagagagcgg gctcgccgtg cagcgcgggc tccggttcgc ccaatggccg aggggaatag
     1141 accttcgacc tagctgcagg ccaccgaccg gccgatgcgt cggtcgcacc cattccctac
     1201 ggtcgttgcg cgatgcaaag actagatctg tcaacggtcc gttacgtcga tgtcgacgcg
     1261 agcctggtca ccttctgctc ctggtgccgc cactcagagt tcgtgcacag cgaacgcgaa
     1321 tcgggactct gcctgttcag cgaatgccaa tgtccgtgtt tcttccccac ggtcgaaagg
     1381 acggaacccc cgacccggag gtagtgcgag cggagggtag gggacaggta tcgtgaccga
     1441 ccaagcaaac tcaaaggtgg cggaagccga agttgtcgac ggcgatgtca tacccgcgcc
     1501 gcctccggcc gctcgccgac gccactgggg ccggatcctc cagatcaccg gcctcctgtt
     1561 catcgccgca gcccttgccc tgggcggcta cctctggtgg aacctctggg gcacggggtt
     1621 cgccgccagg gccgcgcaga acgatctccg accggcgttc gagcgcaacg ttgccagcct
     1681 ccacccgacc gccggccccg agcgggtggc caacgttcct ggtaaggcga tcgcgatcat
     1741 ccgtatcccg aggatcgaga tggacctggt cgtggtcgaa ggcaccaaca ccgaatcact
     1801 caagaaggga cccggccatt actcgcggac ggcatatccc tgggacgaca cgggtagcgt
     1861 cgggatcgcg ggacaccgga cgacgtatgg agccccattc tggtcgctca acgagctccg
     1921 cgaaggcgac aggatcgtgc tcgcgaccga gtatggcatc ttcaattacc gagtaagccg
     1981 gaccgccatc gtcccaccat ccggcatcct cccgtcgggc gactatgtcc tggatcagac
     2041 gaagagtccg acgctcgtgc tcacgacctg caacccgagg ttctcggcat ctgaacgtct
     2101 tgtcgtgttc gccgaccgcg tcggatagat gctctcgctt ggtcgtccaa gtcgacgaat
     2161 tcggccaatg tcgatatcga ggaacgtacg agcgttgttg ccgccccatc ctggcaacgg
     2221 aacggacgac acgctcgagc atctggcgct catgcgctgc gacgttcccg ctccgcggcc
     2281 gcccgtcgca gggcgccctc cgtcgcatct gtgctctcgt ggatgtctgt cagggtacga
     2341 gagagccctc cccgggtgct cagtagaggc cgcccgaacc gcctccgctg ttcgcggcga
     2401 tcaggaccac gaggacggag accactgcca gcgccagacc tactcccaac caggtcttcc
     2461 agttccgctg ggcgcatcgg tcgttcctgc tcgagtcgtc cacctcgacc ccccttttca
     2521 gggcctcatc catctccacg aggatgtacc catgcgacca cgtctgtcgt caccgctagc
     2581 caccatctcc cctcccggca gacccgactt acgccaggag gccggcgccg acgagggacg
     2641 tctctatgcg gtgagcgcgc ggagcgcgtt tcccttggtg ctcgtaccca cggcctcggg
     2701 ggccaccgct cgacctcatt ccggggcggg tcccgcttgg cggacgccga cctcgtcgag
     2761 gtggcgacgg aggtcggccg ggtcgtcgta gacccggtac gcgccggcgc gttcgagctc
     2821 ctcgagcccg tacccgccgg agagcaggcc gatcccgagg gctctggctc ggcgagcggc
     2881 gagcagatcc cagacgctgt cgccgacgac g
//
LOCUS       DASUYD010000002         4261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1543673, whole genome shotgun sequence.
ACCESSION   DASUYD010000002 DASUYD010000000
VERSION     DASUYD010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4261
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543673"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VF195_00025"
     CDS             <1..1024
                     /locus_tag="VF195_00025"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6579254.1"
                     /translation="GLHDAGLTDSARVVVEKPFGHDRESAIALAEEIHQYIDESQLYR
                     IDHYLGKMGLVEILQLRFANAMLEPVWSRNYLSCVEITMAEDFGVEDRGHFYDPVGAL
                     RDVVVNHLMQVVAACAMEAPSRGDPTTIKDAQVALFRAVVEADPAHYVRGQYDGYRDI
                     DGVAKDSSTETFAALRLEIQNWRWSGVPFFIRTGKRLPVLQTELRLVFKHSPWLGFAA
                     LDRRPEPNQLVVRLDPTTGIRLVVEAHRADVAGAAPIQLDMEFSLEGGEGPTPYEVLL
                     HAALLGDSTRFTRQDGVEETWRVFQPLLDSPPPVQPYAPGTWGPDAANGLLEGFGTWH
                     EPWVSQ"
     gene            1069..2916
                     /locus_tag="VF195_00030"
     CDS             1069..2916
                     /locus_tag="VF195_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6579255.1"
                     /translation="MPSPFPPIADYAFLSDCHTGALIAPDGAVDWLCVPRFDSPSIFG
                     SLLDRQAGAFRFGPYGINVPSEIAYEPGTNVLRTTWKTPPGWVVVRDALTMGARAGAD
                     TVTPHTRPPADDDADHTLVRTVECIDGQVEVELVCEPIFDYGRVVASWTVEHRDGHTA
                     DATASDLTIRLQTDLAMGAEGDRVRARHVLNAGDRAFCSLSWGHDLAGPANVDEAEAR
                     IEATVRFWRNWLGRARIPDHRWRDPIQRSALVVKGLTFMPTGATVAALTTSLPETPGG
                     ERNWDYRFTWMRDSTFTLQALHWLNLDWEADEFMQFVADVEPNDDGGMQIMYGIDGRR
                     DLTESTLDDLSGYAGARPVRIGNGAFDQRQNDVYGAVLDSILMHTRRSQRLPRRLWPI
                     VQSQAECATRVWREPDQGIWEARGAPQHYVSSKLMCWVALDRAEKLAQIGGDADLSQT
                     WGKTAEEIHQDILDHGMSDRGVLRQHYDTDALDASTLLAPIFGFLPGTDERMHASVLA
                     IADELTENGYVLRYETDQTDDGMSGKEGTFLICSFWLVSGLAIVGEEQRARDLMERLL
                     RISSPLGLYAEEYDVETGRHLGNTPQAFSHLALIEAAGRIILGERVAEL"
     gene            2942..>4261
                     /locus_tag="VF195_00035"
     CDS             2942..>4261
                     /locus_tag="VF195_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006342070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX6579256.1"
                     /translation="MSDYEVIVIGTGAGGGTLARHLAPSGKRILLLERGGWLAREPQN
                     WSTADVFIDGRYISPDTWYDAKGRTFQPQVHYFVGGATKLYGAALYRLREKDFGELKY
                     PDGISPAWPIGYSDMEQYYTLAEQLYQVRGARGEDPTEPPSSAPYPFPALPHEPRIQQ
                     LSDDLAAAGLHPFHAPCGVLRDEDAPQFSVCVRCGNCDGFPCPLHAKSDAEVMGVRPA
                     LEQPNVTLLTDAPAVRLNTNLEGTSVIEVVIERDSSTETVTGDVVVVSCGAANSAKLL
                     LASANDKQPNGLANGSDQVGRNYMFHYSQAVLALSKEPNPTVFQKTLGVNDFYFGDDD
                     YEYPMGNIQMVGKSQADMYKGEKPIETKLAPTFALRDIATHAVDFWLSTEDLPLPENR
                     VTLEKDGNVKLAYEPSNEESGKRLYHKLKSMLGHLGLHPDHLIPRHAY"
BASE COUNT          726 a         1456 c         1433 g          646 t
ORIGIN      
        1 ggggctccac gacgcgggac tcaccgattc cgcccgtgtc gtggtcgaga agccattcgg
       61 ccacgatcgc gaatcggcga tcgcgctcgc cgaggagatc caccagtaca tcgacgagtc
      121 gcagctgtac cggatcgacc actacctcgg gaagatgggc ctcgtcgaga tcctgcagct
      181 gaggttcgcg aacgcgatgc tcgagccggt ctggagccgc aactatctgt cctgcgtgga
      241 gatcacgatg gcggaagact tcggcgtcga ggatcgcggt catttctacg atcccgtggg
      301 ggcgctccga gatgtcgtgg tcaaccacct catgcaggtg gtcgcggcct gcgcgatgga
      361 ggctccttcc cgcggcgacc ccacgaccat caaggacgcg caggttgctc tgttccgcgc
      421 cgtggtggag gccgaccctg ctcactacgt gcgcgggcag tacgacgggt atcgggacat
      481 cgatggggtc gcgaaggact cgtcgacgga gacgttcgca gcgctccgtc tcgagatcca
      541 gaactggcgc tggtcgggcg tccccttctt catccgaacg ggcaagcgac tgccggtgct
      601 ccagacggag ctccggctcg tcttcaaaca ctcgccctgg ctcgggttcg ctgccctcga
      661 ccgtcgcccc gagccgaacc agttagtggt cagactggat ccgacgacgg gcatccggct
      721 cgtcgtcgag gcccaccggg cggacgtcgc cggcgcggcc ccgatccagc tcgacatgga
      781 gttctcactg gaaggcgggg agggcccgac gccgtatgag gtcctcctcc atgccgctct
      841 gttgggtgac agcacccgct tcacccgaca ggacggggtc gaggagacct ggcgggtgtt
      901 ccagccactg ctggattcgc cgccgcccgt ccaaccctac gctccgggaa cgtgggggcc
      961 ggatgcggcc aacgggctcc tcgaggggtt cggaacgtgg cacgagccct gggtgagcca
     1021 gtgagccgcg gcgccgatcc gaggaccggc ggcgcgcaga gcgccgcgat gccgtcgccc
     1081 ttcccgccga tcgccgacta cgcgttcctg tccgactgtc acacgggagc cctcatcgcg
     1141 ccggacggtg cggtcgactg gttgtgcgtc ccgaggttcg actcgccgag catcttcggg
     1201 agcctgctgg accgacaggc gggggcattc cggttcggcc cgtacggcat caacgtcccc
     1261 tcggagatcg cctacgaacc cgggacgaac gtcctgcgga cgacgtggaa gacgccgccc
     1321 ggctgggtag tcgtccgcga cgcgctcacg atgggagcac gtgccggcgc ggacacggtg
     1381 acgccacaca cccgaccgcc ggccgacgac gacgccgacc acacgctcgt gcgcacggtc
     1441 gagtgcatcg acgggcaggt cgaggtcgag ctcgtctgcg agccgatctt cgactacggc
     1501 cgggtcgtcg cgagctggac ggttgagcac cgagacggac ataccgcgga cgcgacggcc
     1561 agcgacctga cgatcaggct ccagaccgac ctggccatgg gcgcggaagg ggaccgcgtc
     1621 cgcgcccgcc acgtcctgaa cgccggcgac cgcgcatttt gctcgctgtc ctggggccac
     1681 gacctcgctg gcccggccaa cgtcgacgag gccgaggcac gcatcgaggc taccgtccgg
     1741 ttctggcgga attggctggg cagggcccgc atccccgacc accgctggcg agacccgatc
     1801 cagcgctcgg cccttgtggt caaggggctc accttcatgc ccacaggagc gaccgtggca
     1861 gccctcacca cctcgttgcc ggagacgcct gggggcgagc ggaactggga ctatcggttc
     1921 acctggatgc gcgattcgac cttcacgctg caggcgctcc actggctcaa cctggactgg
     1981 gaagcggacg agttcatgca gttcgtcgcc gacgtcgagc cgaacgacga cggcgggatg
     2041 cagatcatgt acgggatcga cggacgtcgg gacctgaccg agtcgacgct ggacgacctg
     2101 tcggggtacg cgggcgcccg tccggtccgg atcggcaacg gcgccttcga ccagcgccag
     2161 aacgacgtct acggggccgt cctcgactcg atcctcatgc acacgcgccg gagccaacgg
     2221 cttccgcgcc gtctgtggcc catcgtccag agccaggcgg agtgcgcgac gcgtgtgtgg
     2281 cgagagccgg accagggcat ctgggaggct cgaggcgcgc cacagcacta cgtgtcctcg
     2341 aagctgatgt gctgggtcgc actcgatcgg gcggagaaac tggcccagat cgggggggac
     2401 gccgacctct cgcagacctg gggcaagact gccgaggaga tccatcagga catcctcgat
     2461 cacgggatga gcgaccgcgg cgtgcttcga cagcactacg acacggacgc gctcgacgcc
     2521 tcgacgctgc tcgcgcccat cttcgggttc cttcccggca ccgacgaacg gatgcacgcc
     2581 tccgtgctcg cgatcgccga cgagctcacc gagaacgggt acgtcctgcg ctacgagacg
     2641 gaccagaccg acgacgggat gtccgggaag gaggggacct tcctgatctg ctcgttctgg
     2701 ttggtctcgg ggcttgccat cgtcggcgag gagcaacggg cccgcgacct gatggagcgt
     2761 ctgctgcgca tctcctctcc actcgggctc tacgccgagg agtacgacgt cgagacgggg
     2821 cggcatctcg gcaacacccc gcaggcgttc tcgcacctcg ccctgatcga ggcggcgggg
     2881 cggatcatcc tcggcgagcg cgtcgccgag ctgtgacgga ccgaacctgg gaggcaagca
     2941 agtgagcgac tacgaggtga tcgtcatcgg taccggcgcg ggcggtggga ccctcgcccg
     3001 acacctggcc ccgtcgggca agcgcatcct cctgctcgag cgcgggggct ggttggcgcg
     3061 cgagccgcag aactggagca cggctgacgt cttcatcgac ggccggtaca tctcgcccga
     3121 cacgtggtac gacgccaagg gaaggacctt ccagccgcag gtccactact tcgtgggagg
     3181 agcaaccaag ctctacggag ccgcgctgta tcgcctgcgg gagaaggact tcggagagct
     3241 caagtatccc gatgggatct cccccgcgtg gccgatcggc tactccgaca tggagcagta
     3301 ctacacgctg gccgagcagc tctaccaggt ccgcggcgcc cggggcgagg acccgaccga
     3361 accgccctcg agcgcgccgt accccttccc ggcgctgccg cacgagccgc ggatccagca
     3421 gctgtcggac gacctcgctg ccgccggcct ccacccgttc cacgcgccgt gcggggtgct
     3481 acgcgacgag gacgcgccgc agttcagcgt ctgcgtccgg tgcgggaact gcgacggctt
     3541 cccgtgcccg ttgcacgcca agtccgatgc cgaggtgatg ggcgtgcgtc ccgctctgga
     3601 acagcccaac gtcaccctgc tcaccgacgc cccggcggtc cgcctgaaca cgaacctcga
     3661 aggtacgtcc gtgatcgagg tggtgatcga gcgcgacagc tcgaccgaga ccgtgaccgg
     3721 cgacgtcgtc gtcgtctcgt gcggtgcggc caactcggcc aagctgctgc tggcgtcggc
     3781 gaacgacaag cagccgaacg gcctggccaa cgggtcggat caggtcggcc gcaactacat
     3841 gttccactac agccaagcgg tgttggccct gtcgaaggag ccgaacccca ccgtgttcca
     3901 gaagaccctc ggggtgaacg acttctactt cggggacgac gactacgagt acccgatggg
     3961 caacatccag atggtcggca agtcccaggc cgacatgtac aagggcgaga agccgatcga
     4021 gaccaagctc gcgccgacgt tcgcgctgag ggatatcgcc acacatgccg tcgatttctg
     4081 gctctcgacc gaggacctgc cgctcccgga gaatcgcgtg acgttggaga aggacggcaa
     4141 cgtcaagctc gcgtacgagc ccagcaacga ggagtccggt aagcgcctgt accacaagct
     4201 gaagtcgatg ctggggcatc tgggtctgca cccggatcac ctcatccccc gacacgccta
     4261 c
//
LOCUS       DASUYD010000003         6064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886 k141_666,
            whole genome shotgun sequence.
ACCESSION   DASUYD010000003 DASUYD010000000
VERSION     DASUYD010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6064
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_666"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VF195_00040"
     CDS             complement(<1..495)
                     /locus_tag="VF195_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6579257.1"
                     /translation="MPLLSLPDGAHVELPEGEPVGAVLAPDAVAARVDGALRDLSFVP
                     AGDASVEPVPPGSDDALHILRHSTAHVLAQAVCRLHPGARYAIGPPIQDGFYYDFDLP
                     TAIGQADLPAIEAEMRAIVEDDQPFLREEVTREDALARLEGQPFKREIIESIGSAQGE
                     VRTGD"
     gene            complement(538..3084)
                     /locus_tag="VF195_00045"
     CDS             complement(538..3084)
                     /locus_tag="VF195_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6579258.1"
                     /translation="MWRAALKSVLARKLRLVLTAMAIVLGVGFVSGTLVLTDTALSAF
                     DDLFGDIFSRTDVVVQAENAFQDIAAGGGGGGGQERNPIPEEVLGDVQALPGVAAANG
                     SVGGFAQVIDPQTDEVISGGGAPTIGSSWDPDVTPFELTGEPPQAEDEVVIDADTVEE
                     AGLEIGDEVRIVTPLGVDPYRLTGVIALPSATTIGGATVASFELSTAQRLFDREGTYD
                     QIYIVAGDGASAGSVAAEVQDVLPPGFQSITATDAAAEQTDQVREGLGFLRTALLVFA
                     FVALFVGAFVIFNTFNIVVTQRTRELGLLRALGASRGQVFTSVVIESLVIGLLGSALG
                     LAAGIGLAVLLKRGLELIGLELPPTPLAIRPTTIIAALVVGTVVTVVASVFPARRASR
                     VAPIEALREGTVAPTASLRRRAIVGGLLTTAGVGAIAAGLFGDVPQPAAIVGLGAQFT
                     FIGVAVLSPFVARPLATAIGAPARRLGMTGRLGRENAKRNPRRTASTAAALMIGLGLV
                     VFVSVFMTTLKASASDTLDETLRADFILSSSGFQPFSPGIANALSQRDEFAAVSPFRQ
                     AGVKIGGGLTFIAGVDETTVDDVVSVPMEAGTPAALAEPGTILIHKGPARARGLEIGD
                     ELPVAFARTGTKDLRIVGIYTDNRLLGDYTVSLEEYEANVAEQLDYLVFVRRANGVSI
                     EQARAGLEEVAGEFPNVEINDQAQFKEEQDRLIDQVFGIVLVLLVLSVIISAFGIANT
                     LALSIYERIRELGLLRAVGMQRSQIGWMIVIEAVIVSLLGAVLGIGIGLLFGWAMQQA
                     LADLGVSRLAIPWGLLGVFAVIAALLGLVASIAPAIRASRIKVLEAISYE"
     gene            complement(3114..3854)
                     /locus_tag="VF195_00050"
     CDS             complement(3114..3854)
                     /locus_tag="VF195_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6579259.1"
                     /translation="MVTETRTAAARAVDLWKVYGEGTAAVEALRGVSVEFAVGEYTAI
                     MGASGSGKSTLLHCMAGLDTPTSGTVWIGDEDLTHLKDHDLTEIRRDRVGFVFQAFNL
                     VPTLTAAENITLPGAIAGRDVDRARFDEVVDTIGLRDRLTHLPSELSGGQQQRVAAAR
                     ALVSRPEIVFADEPTGNLDSRASQELLTFLKAAVTEHGQTIVMVTHDPVGASYANRIV
                     FLADGQVVDEMRDPTAERVLDRMKLLEA"
     gene            complement(4075..4440)
                     /locus_tag="VF195_00055"
     CDS             complement(4075..4440)
                     /locus_tag="VF195_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6579260.1"
                     /translation="MTGRPLDSSPEAAHGRVRHHVPRSTDILPPSELDLATVPLLDIA
                     IADAVAQGGPITIDLSHMTFIDSTGVRSILKALEALPSGCVILHGVHGGIQRVLEITG
                     VDLAPNLHIIPCSVPVKAA"
     gene            complement(4541..4753)
                     /locus_tag="VF195_00060"
     CDS             complement(4541..4753)
                     /locus_tag="VF195_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579261.1"
                     /translation="MPSFRVTYHVVVEAESSDEAIAEAAKAAGSGGLSEPWGRADVTV
                     ERIPDPEAPSGFIAEGVRRGHRKTES"
     gene            5107..5586
                     /locus_tag="VF195_00065"
     CDS             5107..5586
                     /locus_tag="VF195_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579262.1"
                     /translation="MLAAIDWTLVVQSTAAAFTALAAGAAWRAARASRDAVRDHQVGA
                     LIDDLNDLHERLTTLGVVLADDPVFHRFEQCRPGLGRHLAVIDMRLPHTEKLWRTSIP
                     DVIEDTFEEELLSTHASAIHEVEDALAKVRRKRSPWWHRRRSQPVPRNGKDKRPSPS"
     gene            complement(5794..5868)
                     /locus_tag="VF195_00070"
     tRNA            complement(5794..5868)
                     /locus_tag="VF195_00070"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5834..5836),aa:Val,seq:cac)
BASE COUNT         1023 a         2118 c         1999 g          924 t
ORIGIN      
        1 atcgcccgtc cgtacctcgc cttgggccga accgatcgac tcgatgatct cgcgcttgaa
       61 cggctggccc tcgagccggg cgagcgcgtc ctcgcgcgtg acctcttcgc ggaggaacgg
      121 ttggtcgtcc tccacgatcg cacgcatctc tgcctcgatc gcgggcaggt ctgcctggcc
      181 gatcgcggtg ggcagatcga agtcgtagta gaagccgtcc tggatgggcg ggccgatcgc
      241 gtatctcgcg cccgggtgca gccggcacac ggcctgtgcc aagacgtggg ccgtcgagtg
      301 ccggaggatg tggagcgcgt catccgagcc cggcggaacc ggttcgaccg acgcgtcgcc
      361 ggcaggcacg aacgagagat cgcgtagcgc gccgtccacc cgagccgcga ccgcgtccgg
      421 agcgagcacg gcgccgaccg gctccccctc ggggagctcg acgtgggcac cgtccggcag
      481 gctcagtaga ggcacgcgcg ccatgatatc ggcggggctc cgggcctccg acggacgcta
      541 ctcgtacgag atcgcttcga ggaccttgat acgagacgcc cggatcgccg gcgcgatcga
      601 cgccacgagc ccgagcagcg cagcgatcac cgcgaacacg cccaggagtc cccacgggat
      661 cgcgagacgc gacacgccga ggtcggcgag cgcctgctgc atcgcccagc cgaagaggag
      721 cccgatcccg atcccgagca cggcgcccag cagcgatacg atcaccgcct cgatcacgat
      781 catccagccg atctgcgacc gctgcatgcc gacggcgcgc aggaggccga gttcgcggat
      841 ccgctcgtag atcgacagcg ccagggtgtt cgcgatcccg aaggccgaga tgatgaccga
      901 cagcaccagc agcaccaaga cgatcccgaa gacctgatcg atcaggcgat cctgctcctc
      961 cttgaactgc gcctgatcgt tgatctcgac gttggggaac tcacccgcga cctcttcgag
     1021 accggctctc gcctgctcga tggagacgcc gttcgcccgc cggacgaaca ccaggtagtc
     1081 caactgctcc gcgacgttgg cctcgtactc ctccagcgaa accgtgtagt caccgagcaa
     1141 tcggttgtcc gtgtagatgc cgacgatcct caggtccttc gttcctgtcc gagcgaacgc
     1201 caccgggagc tcgtctccga tctccagtcc acgagcccgc gcggggccct tgtggatcag
     1261 gatcgtcccc ggctcggcca gcgcggccgg tgtcccggcc tccatcggca cgctcaccac
     1321 gtcgtcgact gtcgtctcgt ccacaccggc gatgaacgtc agccccccgc cgatcttcac
     1381 gccggcctgc cggaagggcg atacggcggc gaactcgtcg cgctgcgaca gcgcgttcgc
     1441 gatccccggg ctgaagggct ggaaccccga ggaactgagg atgaagtccg cgcggagcgt
     1501 ctcgtcgagc gtgtccgacg ccgacgcctt cagcgtcgtc atgaagacgg agacgaacac
     1561 gaccagtccg aggccgatca tcagtgccgc ggccgtggac gccgtcctgc gagggttgcg
     1621 cttggcgttc tcccgcccga ggcggccggt catcccgagg cgccgggcgg gagctccgat
     1681 cgcggtcgcg agcggcctcg ccacgaacgg ggacagaacc gcgaccccga tgaatgtgaa
     1741 ctgggcgccg agcccgacga tcgcggccgg ctgcgggacg tccccgaaca gccccgcggc
     1801 gatcgccccg acgccggccg tcgtgagcag ccctccgacg atcgctcgac ggcggagcga
     1861 cgcggtcggc gcgacggtcc cctctcgaag cgcctcgatc ggagcgacgc gcgaggcgcg
     1921 acgcgccggg aacaccgacg ccacgacggt cacgacggta ccgaccacga gcgccgcgat
     1981 gatggtggtc ggtcggatcg cgagcggggt cggtggcagc tccagcccga tcagctcgag
     2041 gccccgtttg agcaggaccg cgagcccgat ccccgcggcg agcccgaggg cggagcccag
     2101 gagcccgatc acgagggact cgataaccac ggaggtgaac acctgcccgc ggctggcacc
     2161 cagcgcccgc aagagtccca gctcgcgggt ccgctgcgtc acgacgatgt tgaaggtatt
     2221 gaagatcacg aaggccccga cgaacaacgc gacgaaggcg aacacgagca gcgccgtgcg
     2281 gaggaacccc aagccctctc gaacctgatc cgtctgctcg gcggcggcgt cggtcgccgt
     2341 gatcgactgg aagccggggg ggagcacgtc ctggacctcg gcggcgaccg accccgccga
     2401 cgcgccgtcc cccgcgacga tgtagatctg gtcgtaggtc ccttcgcgat cgaagaggcg
     2461 ttgcgcggtc gagagctcga acgacgcgac cgtcgcgccg ccgatcgtcg ttgctgacgg
     2521 cagggcgatg acgcccgtca gcctgtacgg atcgacaccg agcggtgtga cgatccggac
     2581 ctcgtccccg atctccagcc ccgcttcctc gacggtgtcc gcgtcgatca cgacctcgtc
     2641 ctcggcctgc ggcggctccc ccgtgagctc gaacggcgtg acgtccggat cccacgagct
     2701 tccgatcgtc ggcgcgccgc cgcccgagat cacttcgtcg gtctgcggat cgatcacctg
     2761 cgcgaagccg cccacgctgc cgttcgccgc cgccacccct ggcagcgcct ggacgtctcc
     2821 caggacctcc tccgggatcg ggttccgctc ctggcctcct cccccgccac cgccggccgc
     2881 gatgtcctgg aacgcgttct ccgcctgcac gacgacgtcc gtcctcgaga agatgtcgcc
     2941 gaagagatcg tcgaacgcgg acagcgcggt gtcggtgagc accagcgttc ccgagacgaa
     3001 tccgaccccg agcacgatcg ccatcgccgt cagaacgagc cggagcttcc tcgcgagcac
     3061 gctcttcagc gcggctcgcc acatctcagt gctccctcac gatcgaacgc acctcatgcc
     3121 tccagcaact tcatccgatc gaggacgcgc tcggcggtcg ggtcgcgcat ctcatcgacg
     3181 acctggccgt cggcgaggaa cacgatccgg ttcgcatagc tcgcaccgac cggatcgtgc
     3241 gtgaccatca cgatggtctg tccgtgctcg gtcacggcag ccttgaggaa cgtgagcagc
     3301 tcctgggacg cacgggagtc gaggttcccg gtcggctcgt ccgcgaagac gatctccggg
     3361 cgcgagacga gagctcgcgc tgcagcgacg cgctgctgct gccctccgga gagctcggag
     3421 ggaaggtgcg tcagccggtc gcgcagcccg atcgtgtcaa ccacctcatc gaagcgcgcc
     3481 cggtcgacgt cccgaccggc gatggcgccc ggcagcgtga tgttctcggc cgccgtgagc
     3541 gtcggaacaa ggttgaacgc ctggaagacg aagccaacgc gatcgcgacg gatctccgtg
     3601 agatcgtggt ccttcaggtg cgtgaggtcc tcatccccga tccagacggt tcccgaggtc
     3661 ggcgtgtcga gacccgccat gcaatgcagc agggtggact tgccggagcc ggaggcgccc
     3721 atgatcgcgg tgtactcccc aacggcgaac tccacggaaa cccctcggag cgcctcgacg
     3781 gcggccgtcc cctcgccgta gaccttccag agatcgaccg cgcgtgcggc tgccgtgcgg
     3841 gtttccgtga ccatggtgat cccccctcga ggtgtaagcg gaggagctta ccggtaggga
     3901 tgaacagctg gggtcgacgg accgggaacc agcgtgcttc agatggagcc tccggtcccg
     3961 cgatcagaac ctcggaccag cagggttgcc gagcgccagc cgcgaggctc atgcggggcg
     4021 gatatcccgc gttccacggc cgaacacttg agtggcggtg cagcgaacgg cgggtcaggc
     4081 ggccttgacg ggcaccgagc acgggatgat gtgaaggttc ggcgcgagat cgacgccggt
     4141 gatctccagg acgcgctgga tgccgccatg gaccccgtga aggatgacgc agcccgaggg
     4201 gagcgcctcc agggccttga ggatcgaccg gaccccggtc gagtcgatga acgtcatgtg
     4261 cgagaggtcg atggtgatcg gcccgccctg ggcgacggcg tcggcgatgg cgatgtccag
     4321 cagtggaacc gtggccaagt ccagttcgct tggaggaagg atgtcggtgg accttgggac
     4381 gtgatgccga actcggccat gagctgcctc cgggctcgag tcgagagggc ggccagtcag
     4441 gcatgcgccg acactccagg cgacgggatc gtgctactcg atcgaggata cggtctccgt
     4501 gggcctggtc gtcggcacct gacgagaggc gatgcacctg tcagctctcg gtcttgcggt
     4561 gcccccgtct gacaccttcc gcgatgaagc cggaaggtgc ttcgggatca gggatgcgct
     4621 ccaccgtcac atccgcccga ccccacggct cgctgagccc tccgctcccc gcggctttcg
     4681 ccgcctcggc gatcgcctcg tctgacgact ctgcctcgac cacgacgtga taggtcaccc
     4741 tgaatgacgg catccgatct cctttcgtcg gttcatcagc ctcggcacga ccgagcggac
     4801 ggtcagctta ggagctcgac cgacgaggcg gcagagctca tgagccgatc ggaagaaggc
     4861 catgggtact tcgcgtcgca tgggcgagac gccgacatcg ccgacgagct gaggcgcacc
     4921 cggggacagg ggggagcctc gcgatccgag acgacgatgg tggcggttct ggcgtcgtga
     4981 cgatcgtccc gatcgggcga gaagcgcgac acctgatatc gggacgtcgg tcacggttcc
     5041 caccgcgccg ttgcgggcga gcgcgcagcc cttcctttcc ccgaggccga ccatcatgct
     5101 gcgaacatgc tcgcggccat cgactggacc ctcgtcgttc aatcaacggc ggccgctttc
     5161 acggccctgg cggccggcgc cgcgtggagg gccgctcggg cgagtcggga tgctgttcga
     5221 gatcaccagg tgggtgccct catcgatgac ctcaacgacc tacacgagcg cctgacgacg
     5281 ctcggagtgg tgctagccga cgatcctgtc ttccaccggt tcgaacaatg ccggccaggt
     5341 ctcggccgcc atctggctgt catcgacatg cggctcccgc acaccgagaa gctgtggcgg
     5401 acatccatcc ctgacgtcat cgaggacacc ttcgaggagg agctgctttc gacacacgcg
     5461 agcgccatcc acgaggtcga agatgctctc gccaaggtgc gtcgcaagcg gagcccatgg
     5521 tggcatcgtc ggcgctcgca gcccgttcct cggaatggca aggacaagcg ccccagtccc
     5581 tcgtgaaccg ctcgcatcgg ccttcgtctc gaacgtcttt cgccgggagc gcccgccagg
     5641 atcgcggtac cgagccgtgt agcgacacac tcgggactgt gcgcgcgtcg atgctcgcca
     5701 tgtgtcgcct cgcatcttcg tgacctactc gtgtctcgtt cacgagcgaa ccacgacacg
     5761 cggaggcgga acctgcttct ccaggcacct tcctggtggg cgcgggaggt tttgaacctc
     5821 cgacctcttg cgtgtgaagc aagcgctctc ccactgagct acgcgccctc aactacctgc
     5881 ggagaacgcc ccctctctcc tgttgcacac cgctcgtaca gcacatgaga ttgcagcgct
     5941 gaacgggagg cgatgtcaga gcgttccacg gtccagagga taggcgacct cgagcagatg
     6001 gtcacgtcgt accggcgcca ccctgcgcgc ccagaacgag gctgatcaga cgaactaccg
     6061 cagc
//
LOCUS       DASUYD010000004        29762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1765005, whole genome shotgun sequence.
ACCESSION   DASUYD010000004 DASUYD010000000
VERSION     DASUYD010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 29762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29762
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765005"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..113
                     /locus_tag="VF195_00075"
     CDS             <1..113
                     /locus_tag="VF195_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015796987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HEX6579263.1"
                     /translation="MVFFDRSKNKSGKWCSMNACGNRAKVRAFRARQAAE"
     gene            110..1039
                     /locus_tag="VF195_00080"
     CDS             110..1039
                     /locus_tag="VF195_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HEX6579264.1"
                     /translation="MTTLELPLRAPGGEPVDLWRTLVSHGFAELSPTDLDEERRTLSF
                     TARVPGGRPRRVRISEAGRRTARLEVLGPPPGPKVVRAVLDGAAHVLRLDQDLSPFYL
                     LAEGDPDLAWAAAGAGRMLRSPTVFEDVVKTVCTTNCAWSATVRMVDALVTHLGEPAL
                     GGDGPLTNAFPSPEAMAGSPESFYREVVRAGYRGAYLIGLARAVAGGELDLEGFATAT
                     PTDLSDLELEAALLALPGVGPYAAAHIMMTLGRHSRLILDSWTRPKYTRLVGRKEAVS
                     DRAIEKRFRRYGDFAGLAFWLFVTRDWVDDGPG"
     gene            1254..2510
                     /locus_tag="VF195_00085"
     CDS             1254..2510
                     /locus_tag="VF195_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6579265.1"
                     /translation="MAEGLGAAPARLARRARRLFGYWRSERRTLRQAIVALALSTCAG
                     FVAGLVLGSITGTLEKLPGLLVLIPAAVGMRGTIFGAMGARLGTGVAAGLFEPTLRRG
                     SLLSNNVAVGILSALLTSFYLAALAKLVASAFGEESVISFWDLVTISVIGGVLASSIA
                     LVVTIGIAVLSFRRGWDLDAVSTPMVTAIGDMVTLPALFLASLLVANDTVSGVTAGIC
                     TAATVASLLAAFRAEAAVRRILLEMLAVVALAPLLDIVAGALLQAHGGELRAVPAVLI
                     LIPPFISQAGALGGILSSRLSSKLQLGVITSRGRPEIPALVDGAIVVVLGVAVFTTIG
                     AVSWALSRLTAGPDPGAAALIGATMLAGAIVLPITLVVGYYLAVLTSRVGIDPDNQGV
                     PFITSLLDLAGVAAILLVMRTSGVLP"
     gene            2510..3697
                     /locus_tag="VF195_00090"
     CDS             2510..3697
                     /locus_tag="VF195_00090"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6579266.1"
                     /translation="MDEPRNVKDLLVELKDASELMVDLAYAAVFFNEEKLAQEVERLE
                     QRMSGHLHRLRITAMLAARSQEDAEGMAAVLWIAHAIERVGDAASDIARVVAARLGIP
                     DALRQDLRHADEVTARLRIREDGPGAGRTLRDLSLPTESGMWVVAIRSGMDWRFDPGA
                     DDVISVGDVLLIRGPEEGVNHVRELAGAPPLPEAPTEEPPPLSELDRAVDLLVEMKDL
                     AEAAVGLAYSALLFNNRSLAAEVGTLEARSDILHDELESWVLRAAPEARDPDALRGLL
                     RLGAASEHMCDAARDMTWYVEQGEPLHPVVQLALEETEETSSEVVVERGSRATGHSLK
                     ELRLETETGMFVLAVQRGSRWIYRPRARFTVQEGDRLISVGPEEGEEALAELTRAPFV
                     AHG"
     gene            3743..4294
                     /locus_tag="VF195_00095"
     CDS             3743..4294
                     /locus_tag="VF195_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579267.1"
                     /translation="MEAFVYLRVHPGSIVPVRNQLTSSAVARRSIVVIGDWDVLCLVD
                     GPDLASIGTGVLSEIQMIEGIDRTYTAPVVPPDRLGVVGFGGLPTPQIIRGACYVHVA
                     AEPGAAAGLYERLSEIGGVSGAAVLGGRWDLIVCVPEPWEVASGIVLDKIHALPGVRT
                     TSTLVSVDYEEPEEDRDQFSSWS"
     gene            complement(4338..5276)
                     /locus_tag="VF195_00100"
     CDS             complement(4338..5276)
                     /locus_tag="VF195_00100"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6579268.1"
                     /translation="MGTVLLAAPRGYCAGVERAVDAVERALEQHGGPVHVRKQIVHNL
                     HVVRGLEDKGAVFVDELDEVPEGAVVVLSAHGSSPTVHADAERRELSLIDATCPLVTK
                     VHLEARRFADDDRTILLIGHAGHEEVEGTSGQAPERTILVQSVEEARAVEVPDPDRLS
                     YLTQTTLSVDETSQIVEVLRSRFPAIEAPPREDICYATQNRQDAVKEVARLADVVLVI
                     GSKNSSNSNRLAEVSRERGTPAYLVDDASEVDPTWLDGAEVVGLTSGASAPEWLVTDM
                     IEWLAARGFDRVEQALITLERVRFALPPGVRATTVR"
     gene            5602..6111
                     /locus_tag="VF195_00105"
     CDS             5602..6111
                     /locus_tag="VF195_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579269.1"
                     /translation="MSRGRLGGIWRPAMFAAWVIQPMTTMIRKSRGLVAASILTAVIM
                     SFPNATTSEAMVQRDQLRVVSTKKICPIDWRQSTWHVKRLIRCAAWHHGVSTETALYV
                     AWRESRYRPSAYNAAGGAAGIYQHLLKYWPGRAADFGFRDWSVFNARANIMVTMRMVK
                     HYGWDPWAL"
     gene            complement(6114..6452)
                     /locus_tag="VF195_00110"
     CDS             complement(6114..6452)
                     /locus_tag="VF195_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579270.1"
                     /translation="MCRAVKVLCVAGDEEELSDLRLSTTAAEWELIPGATSETDALGM
                     IDAERPHAMVVFGPYEQLVSLVRERFPAMRIVTDRDTPGASAVATSSDDVREALRSLA
                     RPGGPILEQP"
     gene            complement(6455..7432)
                     /locus_tag="VF195_00115"
     CDS             complement(6455..7432)
                     /locus_tag="VF195_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEX6579271.1"
                     /translation="MEAHVVRGGAIQSGDVAVADLRSTVAGVDDGAWVWVDAVDPDHA
                     DIAEIQRQLDLHDLAVEDVRHRNQRPKLDLYPGHAFAVFRPLAVGPDGLAGSELFVFA
                     SPRFLVTLRFAPVFDLAKAKLRWPIVCALAPGTASALYAIADEIVDDYLEVVEDLEDR
                     ADELENQVFRSDPAPREPGPALQLEILRLRHDAVQLRRHAVPMRQLIDRLADDSDLVT
                     APLVPYLRDITDHLLRTIELTDGVREILTTVVDIRMAQSAHQLNEVMKKLTAWAAIIL
                     VPTLVAGVYGMNFRHMPELHWVVGYPLALGLMAVSSAWLYLGFRKRGWL"
     gene            7535..7954
                     /locus_tag="VF195_00120"
     CDS             7535..7954
                     /locus_tag="VF195_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015307507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6579272.1"
                     /translation="MAPRRETPWTHPVLTQSADERAKKLQNRIADAVTRFAGSMPFVY
                     LHVVWFSLWIALRVEPFPFGLLTMIVSLEAIFLSTFVMISQNRADAKREALAEHQWQL
                     VQAEEKQNEELLEVSRRILELTTEIHRMSKANPAAST"
     gene            complement(7966..8958)
                     /locus_tag="VF195_00125"
     CDS             complement(7966..8958)
                     /locus_tag="VF195_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008474841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX6579273.1"
                     /translation="MVWFVLVLAYLFEFANGWTDAPNSIATVVSTRVMRPHHAVLMAG
                     VLNLVGALVVGTAVAKTIAEGIVDPNIVGLETVAAAVLAATLWALAAQYFGLPSSESH
                     ALIAGLLGAGFAAGGLAALESEGTNKALIGLVTSPLGGFLMGLILMVLIYRIFTRVRR
                     ATVSKVFGRAQVLSAAFMAFSHGTNDAQKTMGIIALTIALNEAGTAGNVGELTIAPWI
                     VVSAALVMGLGTMIGGWRVVRTLGMRMTHLEPVQGFAAETGAATVITVAARLGIPVST
                     THAIGSAIMGVGATKRFSAVRWGVAGQVVAAWVLTWPSCAILGYALQKFFSFVA"
     gene            complement(8958..9584)
                     /locus_tag="VF195_00130"
     CDS             complement(8958..9584)
                     /locus_tag="VF195_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX6579274.1"
                     /translation="MPKLRFRLVPRDESFYEMFVNLAVKVEEGAAAYQDLVVDYQEVT
                     RKADRLKDLEHEGDEVTHEIMKRLNTVFVTPLDHEDIHRLASGLDDVLDHIEAAADLF
                     VLHKIEQPLPEMKAQADILVQTCRVTREALETLPKFKELERYWVEVNRLENEGDRVYR
                     RAIAGLFGGEYKAMDVLKWRDVIDETEGAIDQLEDVSNMLEAIALKHA"
     gene            complement(9705..10604)
                     /locus_tag="VF195_00135"
     CDS             complement(9705..10604)
                     /locus_tag="VF195_00135"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6579275.1"
                     /translation="MEAPRTTEALLDVKSEVRAAGAVVLRRGDGRVQTVLVHRPRYDD
                     WSLPKGKLNDGESFQEAAAREVSEETGLGGRLRAELPPSRYVDQDGRPKVVRYWTMEV
                     REGRDLRPTREVDEAKWFTLAEARDVLSYDRDREVLDAVARLDALAFLIRHAKAGDRE
                     TWTGDDLSRPLSNTGRRQAKGLVRVSADRAIKRILSSPAVRCVETVRPLSEQRGLPIE
                     PRDELLEGAPLYGLLQLLEELRSTPSVLCGHGDLIPAVIEHLEAQGMLFGPDRGWKKG
                     SIWVLEREAGVFVRATYIPPPLG"
     gene            complement(10565..11506)
                     /locus_tag="VF195_00140"
     CDS             complement(10565..11506)
                     /locus_tag="VF195_00140"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX6579276.1"
                     /translation="MTDRDAMALALAEQVDLQLDQLNRLAEGAPALDVIRSAIASSTM
                     KLVSNEPVVRLGEDPGGIHKARVVARRLRSDLLMFRPLLDRDWADALREEIGWLGRLL
                     GPIRDAEVLGARIQQRVAAMVGLEPTSAKTLLDGLESDRLAGRRRLLAGMRSRRYEAL
                     VVRLVRAGREPVSRSEWVDRPAASTRSLMKRPWAKLSSGAKALGPGSSDASLHATRIE
                     TKRVRYGAEALVPVFGERAARFASAAETLQKILGEHQDAVVAMAWLTDRAMEVDDRAV
                     AFTAGRLAELETVSRDRAREAWPKAWKRLEQRKRFWT"
     gene            complement(11503..13551)
                     /gene="ppk1"
                     /locus_tag="VF195_00145"
     CDS             complement(11503..13551)
                     /gene="ppk1"
                     /locus_tag="VF195_00145"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX6579277.1"
                     /translation="MPDPFPRTERFLNRELSWLEFDARVLALAEDDSNRLLERVKFLA
                     IFGSNLDEFFQVRVAGLQEQREAGLGPTSPEGSTPEEQLAGIMEHVAELCRRTDVLWV
                     DDLQPALEKEGVRLVDWAELDVHDRTHLGELFRERIFPVLTPLSVDPAHPFPYISSLS
                     LNLAALVRDPSSGERRFARVKVPPLLPRFIELPDGERFVPIEQVIAAHLEVLFPGMEI
                     VGHSAFRLTRDADFELEEEEGGDLLEAIESVLRRRLRGATPVRLEFDASSSDEVRTLL
                     TRELALLPDEVFVVEGLLDPSALWSLYELERPELKDDPWTPVTQPRLVGVEDFFEVIS
                     KGDLLVHHPYDSFESSVEAFIEQAAQDPAVLAIKLALYRTSEESPIVRALVRAAETGK
                     QVVALVELQARFDERANITQARTLEQAGVHVVYGIVGLKTHAKVCLVVRREASGIRRY
                     AHVGTGNYNPSTARLYEDVGLLTADPELGVDLSDLFNLLTGYSRQQTYRRLLVAPTDL
                     KQRLIGLIRAEAAAGDGSIVLKMNSLVDAEMIDALYEASRAGVGVDLIVRGICCLRPG
                     VPGLSDTIRVRSIVGRYLEHSRIFRFGGLERGYQHFIGSADLMPRNLERRVEVTVPVL
                     DPALRARLDEILQAGLEDDVLAWDLGSDGVWKKVETERGLSAQERLMQVAVERGESRR
                     "
     gene            complement(13645..14343)
                     /locus_tag="VF195_00150"
     CDS             complement(13645..14343)
                     /locus_tag="VF195_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019815032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579278.1"
                     /translation="MSAEEPPTRILVVEDEESLADTVRYNLEREGYAVAVAPDGRRAL
                     ERFRTEDPALVILDLMLPEISGLDVCRQIRQISNVPIIMVTAKDSEADKVSGLELGAD
                     DYVTKPFSVRELVSRVRAHLRRAAMKPEEPEVLLSGGPVQLDTGKHEVIVRGQSVGFP
                     PKEFELLEAFLRRKGRLLTREFLIEEVWGAGYFGDTKTLDVHVKRIRKKVEQDPHEPK
                     HLVTVRGLGYKFVG"
     gene            complement(14340..15032)
                     /gene="phoU"
                     /locus_tag="VF195_00155"
     CDS             complement(14340..15032)
                     /gene="phoU"
                     /locus_tag="VF195_00155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX6579279.1"
                     /translation="MSREHFHEQLERVELELLSMGELASSAVQRSVEALVRHDTAAAE
                     AVIRDDDPIDELYLRIDGGVLEMLALQSPVATQLRLVSAILHSCLHLERIGDQAVNIA
                     KLFLATQDLPPSQTLLQQLDEMGGHVVTMIRTAMEAMRTRDLELCLKLPKMDDPVDRL
                     NRGTHLEALKLAEDPRALDWGLHMNLAARALERVGDNAVDIGEQVGFLITGEFREFTD
                     ASHDVPEPGGDG"
     gene            complement(15029..15853)
                     /gene="pstB"
                     /locus_tag="VF195_00160"
     CDS             complement(15029..15853)
                     /gene="pstB"
                     /locus_tag="VF195_00160"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017583761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEX6579280.1"
                     /translation="MVAVDTTGGSVHADDANPLIFDINGASVFYGDFRAVRDVSLPIR
                     EHEITALIGPSGCGKTTFIRCLNRMNDLIEGARVEGRILYHGVDLYDERVDPVEVRRR
                     IGMVFQKPNPFPKSIYDNVAFGPRIAGFKGNMDDLVEESLQRAALWDEVKKKLKDSGM
                     ALSGGQQQRLCIARAIATRPDVILMDEPCSALDPIATAKIEDLMRDLVSEYTIVIVTH
                     NMQQAARVSDRTAFFTVDVADDGHRTGHIVEFDRTEMIFTNPSDQRTEDYVTGRFG"
     gene            complement(15912..16859)
                     /gene="pstA"
                     /locus_tag="VF195_00165"
     CDS             complement(15912..16859)
                     /gene="pstA"
                     /locus_tag="VF195_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX6579281.1"
                     /translation="MAIAAPRATGTTVELALRGSRSDVRGRLFAAGLLLTLILSLMIL
                     LALLISVVSDAWPVLSSRGTDFLTDNVSRFESRAGVRQGLVGSLMLVAFVGVLAVPLG
                     IGAAVYLQEYARDTRVTRILTANIRNLAGVPSIVYGILGVVVFVQALRSVTGPEAFGR
                     SYISGGLTLTVLVMPFIIIIAMEALRAVPQGIREAAYGVGATRWEVVRSHVLPYAAPG
                     IFTGAILALARAFGETAPLLLVGAVTGYISPGGSGNSLLETLQGAYTSLPTLIFSWAR
                     QPGEEWKGLTAAAIVVLLAVILVVNLAAIVLRNRYERKW"
     gene            complement(16859..17806)
                     /gene="pstC"
                     /locus_tag="VF195_00170"
     CDS             complement(16859..17806)
                     /gene="pstC"
                     /locus_tag="VF195_00170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX6579282.1"
                     /translation="MSDVTGSQDGIREVLRGSVRRRRKEGGIKALLAGAALISIVISV
                     AIVLALIFRALNFLSDVEIGALWAGTWRPRQGQFDLLSLFYGSFVVTGIAMLVATPLG
                     LGAAAYLSEYARPRARRLLKPILETLAGIPSVVIGFFAFIVINPVIVRDIFGATNTFT
                     MMAAGIGVGILTIPLIASVSEDAMFAVPRGLREAAYGIGARRATVTMRVVFPAALSGI
                     VASLILGISRALGETMVVAIAAGATGNATRTLDPLEPNQTLTAAIASLAIGSDQVKTR
                     GSDLNAFDALYLVGLILFLSTLLLNVVSERFVRRFRKAY"
     gene            complement(17883..18914)
                     /locus_tag="VF195_00175"
     CDS             complement(17883..18914)
                     /locus_tag="VF195_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEX6579283.1"
                     /translation="MLKGKRWARMLIGLVALTMVAAACGDDNPSGNGDPSVDAGLTGS
                     ITISGSSTVEPISALVAELFNETNTDVEITVDGPGTGDGFELFCSGEIDISDASRPIE
                     EDEVAACEDGGIEYTELEVALDGVTVMVNAASTLECLTTQDLYAIFGPNSEGVASTGE
                     ANAISERVGGPGSMTDQPLEITAPGEESGTYDAFIELSGIPDYAVEDEVKEADSEALR
                     PDYQSSPNDNVIVQAMQGSPNAIGFAGFAFAEEAGDQVKEIAVDAGDGCVAPTAETIA
                     DGSYPLSRSLFIYPNNQTAADEEAVQAYVDFYLSDEGLTTAVEEAGYIPLPSDRVDAT
                     RSTWESALG"
     gene            19118..20266
                     /locus_tag="VF195_00180"
     CDS             19118..20266
                     /locus_tag="VF195_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579284.1"
                     /translation="MEPVAIVVAVVVVLATAAAAGWRRSARSARTWEERALAAEAIGR
                     EQQLDALEDRKVRDLTLSTMHEGVLLIDSEARVAFANDAISRHLPSSPPTMDSILPFS
                     LRTAIEDSRERREPSSVLVETGVPTRWLRGTITPAADGATLVVIRDVTQQRRLESVRR
                     DFVANASHELKTPAATIQAAAETLKLAAEEDPAAVPRFAQQLEREAVRLSRIVADLLD
                     LSRLESGSSLDDLVSLGAAAREEGQRLEEAADAAGVILEIEAVREQPLRGSQQDLALL
                     VRNLVDNAIRYSHEGGRVRVEIDSDDREVTLRVKDTGIGIPSKDIPRMFERFYRVDRA
                     RSRETGGTGLGLAIVKHVVENHGGTIEVDTELGRGTTFTIRFPAATSA"
     gene            complement(20275..21804)
                     /locus_tag="VF195_00185"
     CDS             complement(20275..21804)
                     /locus_tag="VF195_00185"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX6579285.1"
                     /translation="MQRSRSDDRPFAVVDVGSNSARMIVFRLRPGEHLDVLEDARAPL
                     RLGRELRDDDRLGPAAIERTVEALRDFRAIADGAGAERMIAVATSAVREATDGDRLLA
                     DAREAGVPLEVIDGKTEATFGFLGAVHDLPVLHGVAMDLGGGSMELTGFHDRKFERSW
                     TLPLGSLRVSDRFLVSDPPEDRELKDLRKAVKASLDEAGVGPVEAGGELVGIGGTVRN
                     LAKMDLRRSDHPLRLLHGYELSAASVGDLVEDLASRTMKRRARLQGLNPDRADTIVGG
                     AVVVLAVMDHLGVERVLISSRGLREGLALGDPDTIPTPREVRTISVATLASRFATWDP
                     GAAARRVSLSSQLVEALDLEVPSLVKEMLEHAASLLDIGKAIDHYERWDHAAMIVTAA
                     DLAGFTHQGLVALSAILRLAGEHTSLGPPARLIPPDERPALRKTAVVLALAEELHRRI
                     PAGERATLACSQRVGRFEVRAPVPVRWRPRNISNRFRAAFGAKLAVMPASETAILATP
                     G"
     gene            21889..22569
                     /locus_tag="VF195_00190"
     CDS             21889..22569
                     /locus_tag="VF195_00190"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6579286.1"
                     /translation="MTTLFLIRHGLTAVTGSRLYGRTEGIHLDERGRRQAAALVDRFE
                     GVRLGAIYSSPLERCVETLEPLATARRLAMRVSAALIEMDAGDWTGRTLPSLRRTKLW
                     DTVQRNPSRFHFPSGESFVDAAARLLDEIERIVARHPRGRVVVGTHGDLVGMLVSHYA
                     GAHLDQFQRVLVDPASVSVVHLGDGPPRILLVNDTGSLRRFAAATPRATYAAARAGRG
                     GRGGKLRG"
     gene            22574..23149
                     /locus_tag="VF195_00195"
     CDS             22574..23149
                     /locus_tag="VF195_00195"
                     /inference="COORDINATES: protein motif:HMM:TIGR03847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 domain-containing protein"
                     /protein_id="HEX6579287.1"
                     /translation="MELGPVDRITADAIGEPGARIFYIQARSGAELVTVIVEKQQVQL
                     LAASVLELLEDVPSAELATELGAGSAGTPAPEGSAMALEEPIEPRWRAGRLSIGFDQD
                     RDLFLLEVEEFRPDPEDLEEDDPRSVLGADVGAEPESIRIWASPIQMLALSRHGAEVA
                     AKGRPTCQFCGNPIDAEGHACPAMNGHSKRD"
     gene            23113..23856
                     /locus_tag="VF195_00200"
     CDS             23113..23856
                     /locus_tag="VF195_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO1664 family protein"
                     /protein_id="HEX6579288.1"
                     /translation="MSRDERALEAGLSDPAVPRVLATGELEVLGLLPNASNHTFLAQA
                     RSADEELLAVYKPRRGEAPLWDFPEGTLCAREVAAYVVSRALGWPNVPATVLRDGPYG
                     VGSVQRFMPHDPEEHYFTLAERFPEELRRVAVFDMVVNNADRKSGHCLLGEDGRIFLV
                     DHGVCFSDEPKLRTVIWDFIGDPLEEATRADVRRLAGEIRGGQVRDELANLLAPVELA
                     ALAERADTVAAAVRFPEPGPDRPFPWPPI"
     gene            23862..24377
                     /locus_tag="VF195_00205"
     CDS             23862..24377
                     /locus_tag="VF195_00205"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX6579289.1"
                     /translation="MAHESSTLWRTTTETPLGPYTIFLSDAGVVRTHFDEEGGPDADM
                     DAVEDRRRTAPIRREVDAYFRGALRTFETPADLSAVGEGFTRRVLEMVRTIPYGELWT
                     YGDVAAAAGAARAGRAAGSALARSPIELFVPCHRVVRAGPSLGSYGRHDDRRRFLLQL
                     EKALPGPSLLP"
     gene            24391..25365
                     /locus_tag="VF195_00210"
     CDS             24391..25365
                     /locus_tag="VF195_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579290.1"
                     /translation="MTVRPVSLATLAFLLGAMSCTADPTDGSDVDTTFAAQMASSWHY
                     AGAPQRVQVGIAASDTNGVRVVTQGTVDFTFAYLGTDGSGAPEAGPTVTGEYVPVPGT
                     DASGEAPTIISGASGVYEAEGVAFDRAGIWRATLSPEIDGVARQLTVDFPVTELSDVH
                     IPAPGEPALHTDTLTMESKGRKGAIDSMADVGGEIPDPELHRWSIADAIDRGRPALVL
                     FGTPAYCASRFCGPEVTELQRIAVEHPDRGVFIHVEIWKDFNAQPQVINEGAADWLLR
                     TMPDGSQNMTEPWLYLIGADGVIVDRWGSLFDPADVEAALEALPEMES"
     gene            25400..26353
                     /locus_tag="VF195_00215"
     CDS             25400..26353
                     /locus_tag="VF195_00215"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6579291.1"
                     /translation="MEQTPTMPPPPIRERCYRHPDVETGVHCTRCGRPICPDCMIPAP
                     VGHQCPECVAEARREYRRGPGRQIAVANVKSTSVTKLVLIAIAIGYVWELVIAGGPGS
                     LFEGPSGQALIDAGALVPYSGGSVAQPVGGIVGGEWWRLLSSIFLHAGVIHLGLNAYV
                     LWIFGTAIERDIGRVSTLAVFLVTGVFAGATSFAFASGFRVAVGASGAIFGLVGAFVA
                     YNYLRRSHVMAQARLRSALSMLLINLVIGFTIPVIDWRAHVGGLVAGLVAGFAVDPSR
                     PAAIRRVAAASGLVALLAIAAALVAYRSAQIGADPSILFSS"
     gene            complement(26363..27901)
                     /locus_tag="VF195_00220"
     CDS             complement(26363..27901)
                     /locus_tag="VF195_00220"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6579292.1"
                     /translation="MARASRATSRAPVTKRFPVLPIVSGALVIGVAVLLIAFILSRIE
                     DEQHRGGPLRNDRPPEGSDPINTAFEGLVTFRGNAARSYYGEGPVPLDPVIGWRAPST
                     RLCSVSFVGIDRSEWCGTGWTGQPNVLVGEDGSIEVREGAYDGMYHFLDGLTGLPLRQ
                     PLATRDLAKGSATTDPDGFPLYYAGSRDGRFRIVATDRHARGEPLVLWSIDGVTSVER
                     PLRNDDWDGAALVVDDVLFEGGENGWLYVIRLHRSYDELGLVTVDPEVVATVPGFDDE
                     LLRALGDTEVSIENSVSLHEGILYFANSGGLVQGWDVSDVLAGGSDLERVFRFWTGDD
                     TDATVVIDDVGDLYVASEYQRSGERSSEVGQLMKLSPSRPSDPLVWSIDAREIGFEGA
                     GGSWSTPALYGDLVYFTTAAGRVLAVERDTGDIRWERRIGAPAIGSPVVVDGVLIQGD
                     CAGDLWAWDVSEPEADPVLLWKLHFSDCIESTPAVWRGWIYLGTRQGYLYGLTERGSV
                     ETTP"
     gene            complement(27919..28740)
                     /locus_tag="VF195_00225"
     CDS             complement(27919..28740)
                     /locus_tag="VF195_00225"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEX6579293.1"
                     /translation="MKVAYQGEPGAYSERAVRSLFADAEPLPCDTVRHAFSRVTSGEA
                     SFGVVPLENSQAGSVNETYELLSNTSLLRIVGESIVRVDHALLGVPGTRLEDVRRAHS
                     HWQALAQCEDFLTSMRIEPVPEHDTAGAARKIAETNDPADAAIASVDAAGLAGLTVLA
                     ERIQTEKENFTKFAVIGGTDPGFGRADKTSMVMAVLDEPGSLLGALEPFAARGINLHK
                     LESRPRHGAAFEYLMYVDFMAAADDPEVESALKEVREHTSLLKILGSYRSAGEPV"
     gene            complement(28784..29728)
                     /locus_tag="VF195_00230"
     CDS             complement(28784..29728)
                     /locus_tag="VF195_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6579294.1"
                     /translation="MSEVWLVLAIGCIAGAILLFGIFFDLSQSERRRAVRMLESQVAG
                     TSSENVNLREQHLSESFGARIIVPVVAGATRVARRITPIDARDRLARKLLLAGTPTGW
                     DAERVLAFKIIGLVSGVVLALVVSSVIGDVTPFIRIVLFGLLGFVGFVAPDSMLNRRV
                     DERQKEILRTLSDTLDLLTISVEAGLSLNAAIAQVVQNVPGILSSEFARMLQEIQLGV
                     PRGDAFRNLAERTDVEELNAFALAMIQADVFGVSIASVLRTQAQQLRIKRRQAAEAKA
                     QQTPVKIVFPLILCILPALFVVIVGPGAIQIWEQFINA"
BASE COUNT         4719 a        10435 c         9911 g         4697 t
ORIGIN      
        1 cgatggtctt cttcgatcga tcgaagaaca agagcggcaa gtggtgctcg atgaacgcct
       61 gcggcaatcg cgccaaggtc cgagcgttcc gcgcgcgtca ggcggcggag tgaccacgct
      121 cgaactgccg ctccgggccc ctggcggcga gccggtcgac ctctggagga cgctggtttc
      181 ccacggcttc gccgagctct cgccgaccga cctcgacgag gagcgtcgga cgctctcgtt
      241 cacagcccgc gtgccgggcg ggcggccgcg ccgggtccgg atctcggagg ccggacgccg
      301 cacggcacgc ctcgaggtcc tcggcccgcc gccgggcccg aaggtcgtcc gcgcggtgct
      361 cgacggggcc gctcacgtcc tgcggctcga tcaggacctc tcgccgttct atctcctcgc
      421 cgagggagac cccgacctcg cgtgggccgc cgccggtgcg ggccggatgc tccgctcacc
      481 gactgtcttc gaggacgtcg tgaagacggt ctgcaccacg aactgcgcgt ggagcgccac
      541 ggtgcgcatg gtcgatgccc tcgtcaccca cctcggtgaa cccgctctgg gcggggacgg
      601 cccgctcacg aacgccttcc cctcgccgga ggcgatggcc ggctcgcccg agtccttcta
      661 ccgcgaggtg gtccgggcgg gctaccgcgg cgcctacctg atcgggctcg cacgggcagt
      721 ggcaggaggc gaactcgatc tcgagggctt cgcgacggcg acgcccacgg atctgtcgga
      781 cctcgagctc gaggccgcgc tcctcgcgct ccccggcgtg ggcccgtacg cggcagcgca
      841 catcatgatg acgctcggca gacactcccg cctgatcctg gattcgtgga cgcgcccgaa
      901 gtacacgagg ctggtcggtc ggaaggaggc ggtctccgat cgtgcgatcg agaagcgctt
      961 ccgtcggtac ggtgacttcg ccggcctcgc gttctggctg ttcgtcaccc gcgattgggt
     1021 cgacgacggg ccgggctgac cccgggccta tactgcgcga gccgcccagg cgtcgatcaa
     1081 ccggcccctc acctggcgag gtctcacgga gagcgatcca cacgatgacg gtgcccttat
     1141 gtccccgcgg agccgcgcga cccacgagct cctccgaacc ccctacgccg gggcgactcg
     1201 gccttcgcgc gaggcgggtg ggcgccgggg cctgatctcc gaagcgggct ccgatggctg
     1261 agggcctcgg cgccgcgccc gcccggctcg cgcgccgagc gcgccgcctc ttcggctact
     1321 ggcgctcgga gcgccggacg ctgcgccagg ccatcgtggc cctcgcgctg agcacgtgcg
     1381 ccggcttcgt ggccggactc gtcctcggct cgatcacggg cacactcgag aagcttcccg
     1441 gcctcctcgt gctgatcccg gccgcggtcg gcatgcgcgg aacgatcttc ggcgcgatgg
     1501 gtgcccgcct cggcacgggc gtcgccgccg gcctcttcga gcccaccctc cgtcgcggga
     1561 gcctcctgtc gaacaacgtc gccgtgggga tcctctcggc ccttctgacg tccttctacc
     1621 tcgccgccct cgcgaagctc gtggcctccg ccttcggcga ggaatcggtg atctcgttct
     1681 gggacctcgt gacgatctcc gtgatcggcg gggtgctcgc ctcctcgatc gccctcgtcg
     1741 tgacgatcgg catcgccgtg ctgtcgttcc gcaggggatg ggacctcgac gccgtttcga
     1801 caccgatggt cacggcgatc ggcgatatgg tcacgctccc agcgctcttc ctcgcgagcc
     1861 tcctcgtcgc gaacgacacc gtcagcgggg tgaccgcggg gatatgcacc gccgcgacgg
     1921 tcgcgtccct gctcgccgcc ttccgcgcgg aggcggccgt ccgacggatc ctcctggaga
     1981 tgctggcggt cgtcgcgctc gcgccgctcc tcgacatcgt cgcgggggcg ctgctgcagg
     2041 cgcacggcgg tgagctgcgg gcggtccccg ccgtgctgat cctgatccct ccgttcatct
     2101 cgcaggcggg cgccctcggc gggatcctct cgtcccggct ctcgtccaag ctgcagctcg
     2161 gggtcatcac ctcccggggg cgcccggaga tcccggcgct cgtggacggc gccatcgtgg
     2221 tcgtgctggg ggtggcggtc ttcaccacga tcggcgccgt gtcgtgggcc ctgtccaggc
     2281 tcacggccgg accggatccg ggcgccgccg cattgatcgg cgccacgatg ctcgccggcg
     2341 cgatcgtgct gccgatcacg ctggtggtcg gctactacct cgcggtgctc acctcacgcg
     2401 tcggcatcga cccggacaac cagggcgttc ccttcatcac gtcgctcctg gacctcgccg
     2461 gcgtggcggc gatcctcctc gtcatgagaa catccggggt actcccgtga tggacgagcc
     2521 tcgcaacgtc aaagacctcc tcgtcgagct gaaggacgcc tcggagctca tggtcgacct
     2581 ggcctatgcg gccgtcttct tcaacgagga gaagctggct caggaggtgg agcggctcga
     2641 gcaacggatg agcgggcatc tccatcgact gcggatcacg gcgatgctcg cggctcgcag
     2701 tcaggaagac gcggagggca tggccgcggt gttgtggatc gcgcatgcca tcgagcgcgt
     2761 gggcgatgcc gcctccgaca tcgcgcgggt cgtggccgcg cgtctcggga tcccggacgc
     2821 cctccgacag gatctccgcc acgccgacga ggtgacggcg cggctgagga tccgtgagga
     2881 cggccccgga gcagggagga cgctccgcga tctgtccttg cccacggagt cgggcatgtg
     2941 ggtggtcgcg atccgcagcg gcatggactg gcggttcgat cccggcgccg acgacgtgat
     3001 ctccgtcggg gacgtcctgt tgatccgcgg ccccgaggag ggcgtgaacc atgtccgcga
     3061 gctggccggc gcgcccccgc tccccgaggc ccccaccgag gagccgccac cgctctccga
     3121 gctcgatcgg gccgtcgacc tcctcgtgga gatgaaggac ctcgccgagg cggcggtcgg
     3181 actcgcgtac tcggcgctcc tgttcaacaa ccgctccctc gcagccgagg tcggcacgct
     3241 ggaggcgcgg tccgacatcc tgcacgatga gctggagtcc tgggtcctcc gggcggcgcc
     3301 cgaggcccgc gatcccgacg ccctccgtgg cctcctccga ctcggcgccg cctcggagca
     3361 catgtgcgac gccgcacgcg acatgacctg gtacgtggag cagggcgagc cgctccatcc
     3421 ggtcgtccag ctcgctctgg aggagaccga ggagacgagc tcggaggtgg tcgtggagcg
     3481 cgggtcccga gccacgggtc actccctcaa ggagctgcgt ctcgagacgg agacagggat
     3541 gttcgtgctc gctgtccagc gcggctctcg ctggatctac cgtccccgag cgcggttcac
     3601 cgtccaggag ggcgaccgcc tgatctccgt cggacccgag gagggcgagg aggcgctcgc
     3661 agagctgacc cgggccccgt tcgtcgcgca cggctgagcc gggtccttgt tcggccttcg
     3721 gggctggcct agcctcccct tcatggaggc cttcgtctac cttcgcgtgc atcccggcag
     3781 catcgtgccg gtgcggaacc agctcacatc ctcggcggtc gcgcgccgct cgatcgtcgt
     3841 gatcggtgac tgggacgtcc tctgcctcgt cgacgggccc gacctcgcct cgatcgggac
     3901 cggagtgctc tccgagatcc agatgatcga gggcatcgac cgcacctaca cggcgccggt
     3961 ggtcccccct gatcggctcg gggtcgtggg gttcgggggg ctccccacgc cacagatcat
     4021 ccgaggggcc tgctacgtcc acgtcgcggc cgaacccggg gcggccgccg gactctacga
     4081 gcggctctcc gagatcggcg gggtctccgg ggccgccgtg ctgggtggcc gatgggacct
     4141 gatcgtatgt gtgccggagc cgtgggaggt ggcctcgggg atcgtgctcg acaagatcca
     4201 cgcgcttccg ggcgtccgga ccaccagcac cctcgtctcc gtggactacg aggaacccga
     4261 ggaggatcgc gaccagttca gctcctggtc gtagacctca gcgtacggtc gtagacctca
     4321 gcgtacggtc gtagacctca gcgtacggtc gtagcccgga cccccggagg cagcgcgaac
     4381 cgcacccgtt ccagcgtgat cagcgcctgc tcgacccgat cgaagcctct ggcagcgagc
     4441 cactcgatca tgtccgtcac gagccactcc ggggcggagg ctcctgaggt gagaccgacg
     4501 acctcggcgc cgtcgagcca ggtcgggtcc acctcactcg cgtcgtccac cagataggca
     4561 ggggtccccc gctctcggct gacctcggcc agtcgattgg aattggagga gttcttggag
     4621 ccgatcacga gtacgacatc ggcgagccga gcgacctcct tcacggcatc ctgccggttc
     4681 tgggttgcgt agcagatgtc ttcacgaggt ggagcctcga tcgccgggaa ccgcgaccgc
     4741 aggacctcga cgatctgact ggtctcgtca accgacagcg tggtctgggt gaggtaggag
     4801 agccgatccg ggtcggggac ctccaccgcg cgggcctcct cgaccgactg cacgaggatc
     4861 gtgcgctccg gcgcctgccc gctcgtcccc tcgacttcct cgtgccccgc gtgcccgatc
     4921 agcaggatcg ttcggtcgtc gtcggcgaac cggcgcgcct cgaggtgcac cttggtgacg
     4981 agcggacacg tggcgtcgat cagtgagagc tcccggcgct ccgcatcggc gtggaccgtg
     5041 ggggacgagc cgtgggccga gaggaccacc accgctccct cgggcacctc gtcgagctcg
     5101 tcgacgaaga cggcgccctt gtcctccagt cctcgcacca cgtggaggtt gtggacgatc
     5161 tgcttgcgga cgtgcacggg tcccccgtgc tgctccagag ctcgctcgac ggcgtcgacc
     5221 gcgcgttcga caccggcgca atatccacga ggagctgcca ggaggaccgt acccaccccc
     5281 tcatcgtacc cagcgccgcc ccttcgcccc ggtggcgacc tcgtcgcccg gcgcggaacc
     5341 ggccgaatgc ccgacgcctg ggctcggttg ccgaccatcg gtccgtcggg ctagggtggt
     5401 cgctgtcggc ttcggaccga gtggcccggt cctcctgcgc acgacgcgcg cttcccacct
     5461 tcccagggtg tgttcggcgt gcgcccgtgc gtgggagaag gcctttcgga ccgaggtcga
     5521 cacaggtcac acaggagagg acgagaccag gaggtgatgc gtacacccgc gacctagcgc
     5581 ggaggccggg tccgaggcgg tatgagccgc ggacggctcg gcgggatatg gcggccggcc
     5641 atgttcgccg cgtgggtgat ccagcccatg accacgatga tccggaagag ccgcggcctt
     5701 gtcgcggcat ccatcctgac cgccgtcatc atgagcttcc ccaatgccac gacatcagag
     5761 gcgatggtgc agcgcgatca gctgcgtgtc gtctcgacca agaagatctg cccgatcgac
     5821 tggcgacaga gcacctggca tgtgaagcga ctcatccgct gcgcggcatg gcatcacggc
     5881 gtgagcaccg agacggccct gtacgtcgcg tggcgcgaat cgcgctaccg gccgagcgcc
     5941 tacaacgccg cgggaggagc ggcgggcatc taccagcacc tcctcaagta ctggcccggc
     6001 cgagctgcgg acttcggatt cagggactgg tccgtgttca acgcccgcgc caacatcatg
     6061 gtcacgatgc gcatggtgaa gcactacggc tgggacccct gggcactctg agctcagggc
     6121 tgctcgagta tcggaccgcc cgggcgcgcg agcgatcgga gcgcctcccg cacgtcatcg
     6181 ctggaggtgg cgacggcgct cgccccgggc gtgtcccggt ccgtcacgat ccgcatcgcg
     6241 gggaaccgtt cccggaccaa cgagaccagc tgctcgtagg gaccgaacac caccatcgcg
     6301 tgcggccgct cggcatcgat catccccaac gcatcggtct cactcgtcgc acccgggatg
     6361 agctcccact ccgcggcggt cgtcgagagc cggaggtcgc tgagctcctc ctcgtcgccg
     6421 gcgacgcaga gcaccttgac cgcccgacac acgcctagag ccacccccgc ttccggaacc
     6481 ccaggtagag ccaggcggag gagacggcca tcagcccgag ggcgagcgga tagccgacga
     6541 cccagtggag ctccggcatg tgtcggaagt tcatcccgta gacccctgcg accaaggtcg
     6601 ggacgaggat gatcgccgcc cacgccgtca gcttcttcat cacctcgttg agctggtggg
     6661 ccgactgcgc catccggatg tccacgaccg tggtcaggat ctcgcgcacc ccgtccgtga
     6721 gctcgatggt gcggagcaga tggtcggtga tgtcccggag gtagggcacg agcggcgccg
     6781 tgaccaggtc cgagtcgtcg gcgagtcggt cgatcagctg gcgcatcggg accgcgtgac
     6841 gcctgagttg cacggcgtcg tgacggagac ggaggatctc gagctggagc gcgggacctg
     6901 gctcacgcgg cgccgggtcc gatcggaaga cctggttctc gagctcgtcc gcgcgatcct
     6961 ccagatcctc gaccacctcg agatagtcgt ccacgatctc atcggcgatc gcgtagagcg
     7021 ccgaggccgt gccgggtgcg agcgcgcata cgatcggcca ccgcagcttc gccttcgcga
     7081 ggtcgaagac cggcgcgaac cggagggtga cgaggaagcg aggcgaggcg aacacgaaca
     7141 gctcggaacc cgcgaggccg tcaggtccca cagcgagggg acggaagacg gcgaacgcgt
     7201 gaccgggata gaggtcgagt ttgggccgct ggttgcggtg ccggacgtcc tcgacggcga
     7261 gatcgtggag atccagctgt cgctggatct ccgcgatgtc ggcatggtcg gggtcgacgg
     7321 catcgaccca gacccacgcc ccgtcgtcga cgccggcgac ggtgctccgg agatccgcga
     7381 cggccacgtc gccagactgg atcgccccgc cccggacgac gtgtgcctcc atcctcggag
     7441 cgtaacccgg agccgaccgg aaccgacggc catgccgtca tccgtcgggc cgaaggctag
     7501 ccttgtccgt gacgagcgaa gaagagggag gcagatggcg ccgcgacgcg agactccttg
     7561 gacccacccg gtcctgacgc agtcggccga tgagcgtgcg aagaagctgc agaaccggat
     7621 cgctgacgcc gtcacccgct tcgccggttc gatgccgttc gtctacctcc acgtcgtctg
     7681 gttctcgctg tggatcgccc tccgtgtgga gccgttcccg ttcggcctgc tgacgatgat
     7741 cgtgtctctg gaggcgatct tcctgtcgac gttcgtgatg atcagccaga atcgggcgga
     7801 cgcgaagcgc gaggcgctcg cggagcacca gtggcagctc gtgcaggccg aggagaagca
     7861 gaacgaggag ctcctcgagg tctcacgtcg gatcctcgag ctcacgacgg agatccatcg
     7921 gatgagcaag gcgaacccgg cagcctccac ctgatccggc cgcgctcagg ccacgaagga
     7981 gaagaacttc tgtaacgcgt acccgaggat ggcgcacgac ggccaggtca ggacccacgc
     8041 ggcgaccacc tgccccgcca cgccccatcg aacggccgag aaacgcttgg tcgcgccgac
     8101 ccccatgatc gcggagccga tcgcgtgggt cgtcgagacg gggataccca ggcgggccgc
     8161 aacggtgatc acggtggcgg cccccgtctc ggccgcgaac ccttggacgg gttctaggtg
     8221 ggtcatgcgc atccccaacg tccggacgac tcgccaccct ccgatcatcg tgccgagtcc
     8281 catcacgagc gcggccgaga cgacgatcca cggtgcgatc gtgagctcac cgacgttccc
     8341 ggccgtccct gcctcgttga gggcgatggt gagggcgatg atgcccatcg tcttctgcgc
     8401 gtcgttcgtt ccgtggctga atgccatgaa cgcggccgag agcacctgcg ccctgccgaa
     8461 caccttcgag acggtggcac gacggacgcg cgtgaagatc cgatagatca acaccatcag
     8521 gatcagcccc atcaggaacc cgccgagtgg cgaggtcacg aggccgatga gcgccttgtt
     8581 ggtgccctcg ctctccaaag ccgccaatcc acccgcagcg aatcccgcac cgagcaggcc
     8641 cgcgatgagg gcgtgggatt cgctcgacgg aagtccgaag tattgggctg cgagcgccca
     8701 gagcgtcgcg gccagcaccg cggccgcgac cgtctcgagg ccgacgatgt tcgggtccac
     8761 gatcccttcc gcgatcgtct tggcgaccgc ggtccccacc accagtgcgc cgaccaggtt
     8821 cagaaccccg gccatcaaga ccgcgtgatg cggtcgcatc acccgcgtgg acacgacggt
     8881 ggcgatcgag ttcggagcgt cggtccaccc gttggcgaac tcgaacagat aggcgaggac
     8941 gagaacgaac cagaccatca ggcgtgcttc aaggcgatcg cctcgagcat gttggaaacg
     9001 tcctcgagct ggtcgatggc tccttccgtc tcgtcgatga cgtcccgcca cttcaggacg
     9061 tccatcgcct tgtactcacc accgaagagc cctgcgatgg cccggcggta gacccggtcc
     9121 ccctcgttct cgagtcgatt gacctcgacc caataccgtt cgagctcctt gaacttcggg
     9181 agcgtctcga gtgcctcccg cgtgaccctg caggtctgca ccaggatgtc ggcctgcgcc
     9241 ttcatctcgg gcagcggctg ctcgatcttg tggagcacga acagatccgc ggccgcctcg
     9301 atgtggtcga gcacatcgtc cagaccggag gcgagccggt ggatgtcctc gtggtccaaa
     9361 ggcgtcacga agacggtgtt cagccgcttc atgatctcgt gcgtgacctc gtcgccctcg
     9421 tgttcgaggt ccttcaatcg gtcggccttc cgcgtcacct cctggtagtc cacgacgagg
     9481 tcctggtacg cggcggcccc ctcctcgacc ttgaccgcca ggttgacgaa catctcgtag
     9541 aagctctcgt cccggggaac gagcctgaac cggagcttgg gcatccttgt gaccctttca
     9601 tggtcggccg atggtggata tcacgtgcgc gagggctgag caatggccgg atcaggggtc
     9661 gcggcgctca ctcagacggt tgccggcgag gagcttcctt cgcgtcaacc caggggcggg
     9721 gggatatagg tcgcgcgaac gaagacgccg gcttcacgct ccaacaccca gatagatccc
     9781 ttcttccacc cacgatccgg tccgaagagc atcccctgtg cctcgagatg ctcgatgacc
     9841 gcgggtatca gatctccatg gccgcacagg acggacggcg tggatcggag ctcctcgagc
     9901 agctggagca ggccgtagag tggcgccccc tcgaggagct catctcgtgg ctcgatcggg
     9961 agcccgcgct gctccgacaa tggtctcacc gtctccacgc agcggacggc cggactgctc
    10021 aagatccgct tgatggctct gtcggccgat acccggacga gacctttggc ttgccgacga
    10081 cccgtgttcg agagcggtcg cgaaagatcg tcaccggtcc acgtctcgcg atcgccggcc
    10141 ttcgcgtgtc ggatgagaaa cgcgagagcg tcgagacgcg ccaccgcgtc gagcacctct
    10201 cggtcccggt cgtagctaag gacgtcacga gcctcggcga gggtgaacca cttcgcttca
    10261 tccacctctc tggtcggacg gaggtcgcgc ccctcgcgta cctccatggt ccagtagcgg
    10321 acgaccttgg gtctcccgtc ctgatcgacg taccgagatg gtggcagctc ggcgcgcaac
    10381 cgacccccga gccccgtctc ctccgacacc tctcgcgccg cggcctcctg gaacgattcg
    10441 ccatcgttca gcttcccctt cgggagcgac cagtcgtcgt aacgaggtcg gtgcacgagc
    10501 accgtctgca ctcgtccgtc cccacggcga agcactaccg cccctgccgc gcggacctcg
    10561 ctcttcacgt ccagaagcgc ttccgttgtt cgaggcgctt ccacgccttc ggccaggcct
    10621 ctcgcgctcg gtcgcgtgag acggtctcga gctccgcgag gcgtccggct gtgaacgcca
    10681 ccgcccgatc gtcgacctcc atagcccggt ccgtgagcca ggccatcgcc acgacggcgt
    10741 cttgatgctc gccgaggatc ttctgcagcg tctccgccgc ggaggcgaaa cgagccgccc
    10801 gctccccgaa cacggggacg agtgcctcag ccccgtagcg cacccgcttc gtctcgatcc
    10861 gggtggcgtg gagggaggcg tcgctcgagc ccggtccgag cgccttcgca ccgcttgaga
    10921 gcttcgccca gggacgtttc atcagtgacc gtgtgctggc ggccggtcga tccacccact
    10981 cgctcctcga caccggttcc cggcctgcgc gcacgagcct cacgacgagc gcctcgtacc
    11041 ttcgagaacg catgcccgcg aggagcctgc gccgaccggc gagacgatca ctctcgagac
    11101 cgtcgaggag ggtcttcgcc gatgtcggct cgagcccgac catggccgcg acccgctgct
    11161 ggatcctagc gccgagtacc tccgcgtcgc ggatcggtcc gaggagccgg ccgagccatc
    11221 cgatctcctc tcgcagcgcg tcggcccagt ctcggtcgag gagaggccgg aacatcagga
    11281 gatccgagcg tagccgccgg gcgacgacgc gcgccttgtg gatacctcct gggtcttcgc
    11341 cgagccggac gacaggctcg ttcgacacga gcttcatcgt ggagctggcg atcgcagatc
    11401 tgatgacgtc gagcgcaggc gccccttccg cgagccgatt gagctgatcc agctgcagat
    11461 ccacctgctc cgcgagagcc aacgccatcg cgtcgcgatc ggtcatcgac ggctctcccc
    11521 tcgctcgacg gcgacctgca tgagacgctc ctgcgcgctc aggccacgct ccgtctccac
    11581 cttcttccag acgccgtcgg acccgagatc ccaggccagc acatcgtcct ccagccccgc
    11641 ttggaggatc tcgtcgagac gcgcacgcag tgccggatcg agcaccggaa cggtgacttc
    11701 gacgcgtcgc tcgaggttcc gaggcatcag gtcggctgag ccgatgaagt gctggtagcc
    11761 acgctcaagg cctccgaacc ggaagatcct cgaatgctcc aggtagcggc cgacgatcga
    11821 ccggacccgg atggtgtcgg agagaccggg cacgccaggc cggaggcagc agatccctcg
    11881 gacgatcagg tccactccga ccccggcacg cgacgcctcg tagagcgcgt cgatcatctc
    11941 cgcatccacc aggctgttca tcttcaggac gatggatccg tcccccgccg ccgcctccgc
    12001 gcggatcaat ccgatcaatc tctgtttgag atccgtgggt gcgaccagca ggcgccggta
    12061 cgtctgctgt cggctgtagc cggtcagcag gttgaacagg tcgctgagat cgacgccgag
    12121 ttccggatcc gcggtcagca ggcctacatc ctcgtagagg cgggcggtgc tcgggttgta
    12181 gttgccggtg ccgacgtgcg cgtaccgccg gatgccggat gcctcgcgac gaacgaccag
    12241 gcacaccttg gcgtgggtct tcagtcccac gatgccgtag accacgtgga cacctgcctg
    12301 ttccagcgtg cgagcctgcg tgatgttggc gcgctcgtcg aagcgtgcct ggagttcgac
    12361 gagcgccacg acctgtttgc ccgtctcggc ggcgcgcacc agagcgcgga cgatcgggct
    12421 ctcctcggag gttcggtaga gggcgagctt gatcgcgagg acggcggggt cctgcgccgc
    12481 ctgttcgatg aacgcctcga ccgagctctc gaacgaatcg tacggatggt ggacgaggag
    12541 gtcgcccttc gagatgacct cgaagaagtc ctccactcct accaagcgag gctgggtcac
    12601 aggggtccac gggtcgtcct tgagctccgg acgctcgagc tcgtagaggg accacagcgc
    12661 ggaggggtcg aggagcccct ccacgacgaa cacctcgtcg ggcaggagcg cgagctctcg
    12721 cgtcaacagc gtccgtacct cgtcgctgga agacgcgtcg aactcgagcc gaaccggggt
    12781 cgcccctcga agtcggcgcc gcaggacgga ttcgatcgcc tcgagcaggt ccccgccctc
    12841 ctcctcctcg agttcgaagt ccgcgtctcg ggtcaggcgg aacgccgaat gaccgacgat
    12901 ctccatgccc gggaacaaga cctcgaggtg cgctgcgatc acctgctcga tcgggacgaa
    12961 ccgctcgccg tccggcaact cgatgaaccg agggagcaga ggcggcacct tcacgcgcgc
    13021 gaagcgccgc tctccgctgg acgggtcgcg gacgagggcg gcgagattga gcgacaggga
    13081 cgagatgtat gggaacggat gcgccgggtc gaccgagagc ggggtgagca cggggaagat
    13141 ccgctcgcgg aacagctctc cgaggtgcgt acggtcgtgg acgtcgagct cggcccaatc
    13201 gacgagcctc actccctcct tctcgagcgc cggctggagg tcatcgaccc acaacacgtc
    13261 cgttcgccgg cagagctcgg ccacgtgctc catgatgccg gcgagttgct cctcgggtgt
    13321 cgatccctcg ggcgacgtag ggcccaggcc cgcttcccgc tgctcctgga ggcccgcgac
    13381 acgcacctgg aagaactcgt ccaggttgga gccgaagatc gcgaggaact tcacccgctc
    13441 gaggagccga ttcgagtcgt cctcggcgag cgcgaggacg cgagcatcga actccagcca
    13501 tgagagctcg cggttgagga aacgctcggt gcgggggaag ggatccggca tcggagcgtc
    13561 gatgctagcg gctcgctgcg tcggatcgcc agtcggccga acggagcgcg ccgggctctc
    13621 gggaaggcct ctcgtgacga cctctcagcc gacgaacttg tagccgaggc cgcgcaccgt
    13681 gaccaggtgc ttcggctcgt gcgggtcctg ctcgaccttc ttcctgatcc tcttcacgtg
    13741 gacgtccagc gtcttggtgt cgccgaagta gcctgcgccc cagacctcct cgatgaggaa
    13801 ctccctcgtg agcagtcgac cttttcgccg caggaacgct tcgaggagct cgaactcctt
    13861 cggagggaac ccgaccgact ggccgcgtac gattacctcg tgcttcccgg tatcgagctg
    13921 gaccgggcct ccgctcaaca gaacctccgg ttcctcgggc ttcatagcgg ctcgacggag
    13981 atgcgctcgc accctggaca cgagctctct gacggagaac ggcttcgtga cgtagtcgtc
    14041 cgcgccgagc tcgaggcccg agaccttgtc cgcctccgaa tccttggcgg tcaccatgat
    14101 gatcggcacg ttggagatct gccggatctg gcggcagaca tcgagcccgg agatctcggg
    14161 gagcatcagg tccaagatca ccagtgccgg atcctccgtg cggaaccgct ccagcgcacg
    14221 tcgaccgtcg ggagccacgg ccacggcata tccctcccgc tccaggttgt agcggaccgt
    14281 gtccgcgagc gactcctcgt cctcgaccac gaggatgcgg gtcggtggct cctccgcgct
    14341 catccgtcgc ctcccggttc gggcacgtcg tgggacgcgt cggtgaactc acggaactcg
    14401 ccggtgatca ggaacccgac ctgctccccg atgtcgaccg cgttgtcgcc gacccgttcg
    14461 agcgctcgag ccgcgaggtt catgtggagc ccccagtcga gcgcgcgcgg atcttcggcg
    14521 agcttcaggg cctcgagatg ggtccctcgg ttcagacgat ccacggggtc gtccatcttc
    14581 ggaagcttca agcacagctc gaggtcgcgc gtccgcatcg cctccatcgc ggtccggatc
    14641 atcgtgacca cgtgaccgcc catctcgtcg agctgctgga ggagggtctg actgggcggc
    14701 aggtcctgcg tcgcgaggaa gagcttggcg atgttgacgg cttggtcccc gatccgttcg
    14761 aggtgcaggc agctgtgcag gatggcggag acgagccgga gctgggtggc cacgggcgat
    14821 tgcagggcga gcatctccaa cacgccgccg tcgatgcgaa ggtagagctc gtcgatggga
    14881 tcgtcgtctc ggatgacggc ctcggcggcc gcggtgtcgt gacggaccag cgcctcgacc
    14941 gagcgttgga ccgcggagga ggcgagctct cccatggaca gcaactcgag ctccacccgc
    15001 tcgagctgct catggaagtg ctcgcggctc atccgaacct ccccgtgacg tagtcctcgg
    15061 tgcgttggtc ggacgggttc gtgaagatca tctccgtccg atcgaattcg acgatgtgcc
    15121 ccgtccggtg gccgtcgtca gccacgtcca ccgtgaagaa ggcggtccgg tcggacaccc
    15181 gggcggcctg ctgcatgttg tgcgtcacga tcacgatcgt gtactcggac acgagatcgc
    15241 gcatcaggtc ctcgatcttg gctgtggcga tcgggtcgag ggccgaacac ggctcgtcca
    15301 tcaggatcac gtcggggcgg gtcgcgatcg cccgggcgat gcacaggcgc tgttgctgcc
    15361 cccccgagag cgccatcccc gagtccttca gcttcttctt gacctcgtcc cagagtgccg
    15421 ctcgctgcag cgattcctcc accaggtcgt ccatgttccc cttgaagcct gcgatgcggg
    15481 gtccgaacgc gacgttgtca tagatcgact tcgggaacgg attcggcttc tggaagacca
    15541 tcccgatccg tcgacggacc tccaccggat ccacccgctc gtcgtacagg tcgaccccgt
    15601 ggtacaggat cctcccctcg acccgagcgc cctcgatcag gtcgttcatg cggttgaggc
    15661 atcggatgaa ggttgtcttg ccacatcccg acggcccgat gagcgccgtg atctcatgct
    15721 cacggatcgg cagcgagaca tcccggaccg cgcggaaatc cccgtagaac acagaggccc
    15781 cgttgatatc gaagatcaat gggttcgcgt cgtcggcgtg aacggacccg ccagtggtgt
    15841 cgacggccac gatcttctcc tgttcggtgc ccgtagggtc cggcttcctt accacgccag
    15901 tatccatcgg gtcaccactt ccgctcatat cggttcctta agacgatcgc cgccaggttc
    15961 acgacgagga tcaccgcgag caacaccacg atcgcagccg cggtgagccc cttccactcc
    16021 tcgcccggct gacgagccca cgagaagatg agcgtcggca gcgatgtata cgcgccttgg
    16081 agggtctcga ggaggctgtt cccactccca ccggggctga tgtacccggt aaccgcgccg
    16141 actagcagca gcggtgccgt ctcgccgaac gcgcgggcca gcgccaggat cgccccggtg
    16201 aagatgccgg gcgccgcata cggcagcacg tggctccgga cgacttccca ccgcgtcgcg
    16261 ccgacgccgt acgcagcctc gcggatcccc tgcggaacag ctcgcagcgc ctccatcgcg
    16321 atgatgatga tgaacggcat cacgagcacg gtaagggtga ggccgcccga gatgtagctc
    16381 cgcccgaacg cctccggtcc ggtcaccgac ctgagcgcct gcacgaagac cacgacgccg
    16441 aggatcccgt agacgatcga tgggacgcca gccaagttcc ggatgttcgc cgtcaggatc
    16501 ctcgtgaccc gggtgtcacg ggcgtactcc tggaggtaga cggctgcccc gatccccagc
    16561 gggacggcga gaacgccaac gaaggcgacc agcatcaacg accccacgag cccctgccgg
    16621 acgcccgccc gactctcgaa gcgcgagacg ttgtcggtca agaagtccgt tccgcggctg
    16681 gacagaaccg gccacgcatc ggatacgacg gagatgagca gagccaagag gatcatcagt
    16741 gacaggatca gcgtgagcaa gagcccggct gcgaagagtc ggccgcggac gtcgctccga
    16801 ctcccgcgga gggcgagctc caccgtcgtc ccggtcgccc ggggggcggc gatcgccatc
    16861 aatacgcctt ccggaagcgt cgcacgaacc gctcgctgac gacgttcagc aggagcgtgg
    16921 acaggaacaa gatcaacccg acgaggtata gagcgtcgaa cgcgttgaga tccgaccctc
    16981 gggtcttgac ctggtcggag ccgatcgcga gcgaggcgat ggcggcggtc agcgtctggt
    17041 tcggctccaa gggatccagc gtccgcgtcg cgttcccggt ggcgccagcg gcgatcgcaa
    17101 cgaccatcgt ctcgcccagc gcccgggaga tcccgaggat cagcgaggcg acgatcccgg
    17161 agagggccgc gggaaacacg acccgcatcg tcacggtggc gcggcgagcg ccgatcccat
    17221 acgccgcctc acgcagcccc cgggggacgg cgaacatcgc atcctccgag accgaggcga
    17281 tcagggggat cgtgagtatg ccgacgccga tcccggcggc catcatggtg aacgtattcg
    17341 tcgccccgaa gatgtccctg acgatgaccg ggttgatcac gatgaacgcg aagaacccga
    17401 tcacgacgct ggggatcccg gcgagcgtct cgaggatcgg cttgaggagc cggcgggccc
    17461 gcggccgagc gtactccgag aggtatgcgg ccgcgccgag cccaagcggc gtcgcgacga
    17521 gcatcgcgat gccggtgacc acgaacgacc catagaacag cgagagcagg tcgaactgcc
    17581 cctgtcgcgg ccgccacgtg ccggcccaga gcgctccgat ctcgacgtcg gagaggaagt
    17641 tcaacgcgcg gaagatcaac gccaggacga tcgcaacgct gatcacgatc gagatcaggg
    17701 cggctccggc gagcaacgcc ttgatcccgc cctccttgcg gcgccgtcgg acgctgcctc
    17761 gcagcacctc gcggatccca tcctggcttc cagtgacgtc gctcatcggc ccgaaaggct
    17821 atcgtcgccg ggcgaagcgt gagcgccgct gatgcggcgc ccacgctcca agggccctcg
    17881 actcatccga gcgccgactc ccacgtggag cgcgtcgcgt cgacacggtc agacgggagc
    17941 gggatatacc cggcctcctc gacggccgtc gtgagacctt cgtcggagag gtagaagtcc
    18001 acataggcct gcaccgcttc ctcgtcggcc gccgtctggt tgttcgggta gatgaagagc
    18061 gatcgagaca gcgggtagct gccgtcggcg atcgtctctg ccgtcggtgc gacacatccg
    18121 tcgccggcat cgaccgcgat ctccttcacc tgatcgcccg cctcctcggc gaacgcgaag
    18181 ccggcgaaac cgatggcgtt ggggcttccc tgcatcgcct ggacgatcac gttgtcgttc
    18241 ggtgacgact ggtagtcggg tcggagggcc tcggagtctg cttctttgac ctcatcctcg
    18301 accgcgtagt cggggatccc ggacagctcg atgaacgcgt cgtacgtacc cgactcctca
    18361 ccaggcgcgg tgatctccag cggctggtcc gtcatggaac cgggaccgcc gacgcgctcc
    18421 gagatcgcat tcgcctcgcc cgtcgaggcg acaccctctg agttgggccc gaagatcgcg
    18481 tagaggtcct gcgtcgtcag gcactcgagt gtgctcgcgg cgttcaccat cacggtgacg
    18541 ccgtccaaag ccacttcgag ctccgtgtac tcgatcccgc catcctcgca ggccgcgacc
    18601 tcgtcctctt cgatcgggcg ggaggcgtcg gagatgtcga tctcgccgct gcagaagagt
    18661 tcgaacccgt cgccggtgcc tggaccgtcc acggtgatct cgacatcggt gttcgtctcg
    18721 ttgaagagct ctgcgaccag agccgagatg ggctcgacgg tggatgatcc cgagatcgtg
    18781 atcgatcccg tgagccctgc gtccacggag ggatctccat ttccgctcgg gttatcgtca
    18841 ccgcacgcgg ccgcgaccat cgtcagtgcc accagaccga tgagcatccg cgcccaccgt
    18901 ttccctttca gcatgctgcc tccccctgtg atcggtagct gccgtcaggc tagggagact
    18961 ggatgaagcg cccacggctc gaaggtgaac ggacgatgaa cccggctctc ccgtgatccc
    19021 ggctgatcgg gagtgggggg ctcggcgaag cgggccggtg gatgaatcat cactgaacag
    19081 cacatgaacg aggtgacccc gggcctacac tcggctcgtg gagcccgtcg cgatcgtggt
    19141 ggccgtggtt gtcgtcctcg ccaccgccgc cgccgccggt tggcggcgct ccgcgcgatc
    19201 ggcgcgcacg tgggaggagc gagccctcgc cgccgaggcg atcggacgag aacagcagct
    19261 cgacgcgctc gaggacagga aggtccgcga cctcacacta tcgacgatgc acgaaggggt
    19321 gctcctgatc gactccgaag cgcgggtcgc cttcgcgaac gacgcgatca gccgccacct
    19381 tccctcatcg cccccgacga tggactccat ccttcccttc tcgctccgga ccgcgatcga
    19441 ggacagtcgc gagcgccgag agccgagctc cgtccttgtg gagaccggtg tgccgacccg
    19501 ttggctccgc ggaaccatca cccccgccgc cgacggggca accctggtcg tgatccgcga
    19561 cgtcacccag cagcgacggc tggaatccgt gcggcgcgac ttcgtcgcca acgcgtcgca
    19621 tgagctgaag acgcctgcgg ccacgatcca ggccgctgcc gagacgctca aactggccgc
    19681 cgaggaagat cccgccgccg tccctcggtt cgcgcagcaa ctggagcgcg aggccgtccg
    19741 gctctcccgc atcgtggccg acctcctgga cctttctcgt ctcgagtccg gcagctccct
    19801 cgatgacctc gtgtcgttgg gggccgccgc gcgtgaggaa gggcagcgcc tcgaggaggc
    19861 agccgacgcg gccggcgtca tcctggagat cgaagccgtc cgcgagcaac ccctccgcgg
    19921 atcgcagcag gatctcgcct tgctggtgcg gaacctcgtg gacaacgcga tccgttacag
    19981 ccacgaaggg ggccgggtcc gcgtcgagat cgactccgac gaccgcgagg tgaccttgcg
    20041 cgtgaaggac acggggatcg gcatcccctc caaagacatc ccccggatgt tcgaacgctt
    20101 ctaccgcgtc gatcgggctc ggtcgcgaga gacgggcggc acgggactcg gtctggcgat
    20161 cgtgaagcac gtggtcgaga accacggagg cacgatcgag gtcgacaccg agctcgggcg
    20221 ggggacgacg ttcacgatcc ggttccccgc cgccacctca gcctgaccga aggatcagcc
    20281 cggtgtggcg aggatcgccg tctcggacgc cggcatgacc gcgagcttcg ccccgaaagc
    20341 cgcacggaaa cggttggaga tgttcctcgg ccgccatcgg acaggcacgg gcgctcggac
    20401 ctcgaagcgg cccacgcgtt gcgagcacgc gagggtggcg cgctccccgg cggggatccg
    20461 tcggtgcagc tcttcggcga gggcgaggac cacggccgtc ttgcggagcg ccggccgctc
    20521 atcgggcggg atcaagcgtg cgggggggcc gaggctcgtg tgctctcccg cgagacgaag
    20581 gatcgccgac agggcgacca acccctggtg ggtgaatccc gcgaggtcgg ccgcggtcac
    20641 gatcatcgcg gcatggtccc atcgctcgta gtgatcgatc gccttgccga tgtcgagcag
    20701 cgaggcggcg tgctcgagca tctccttcac gagcgagggc acctccaggt ccagcgcctc
    20761 gaccagttgg gacgagaggg agacacggcg cgccgccgcg cccggatccc acgtagcgaa
    20821 ccgggaggcg agggtggcga ccgagatggt ccgaacctca cggggcgtgg ggatggtgtc
    20881 cggatctccg agcgcgaggc cttcgcggag accgcggctc gagatcagca cccgctcgac
    20941 gccgaggtgg tccatcacgg cgagcacgac gacggcccca ccgacgatgg tgtcggcccg
    21001 atccgggttc aacccttgca gccgcgcgcg tcgcttcatg gtcctgctcg cgaggtcctc
    21061 gacgaggtcc cccacgctgg ccgccgagag ctcgtatccg tggaggagcc ggagcggatg
    21121 gtcggatcga cgcaggtcca tcttggcgag gttacgcacc gtgccaccga tcccgaccag
    21181 ttccccgccg gcctccacgg gaccgacgcc cgcctcatcc agcgacgcct tcacggcctt
    21241 gcggagatcc ttcagctcgc ggtcctcagg aggatccgag accaggaacc ggtcgctcac
    21301 gcgcagcgac ccgagcggaa gcgtccacga cctctcgaac ttccggtcgt ggaagccggt
    21361 cagctccatg ctccccccgc cgagatccat cgcgacgccg tggaggaccg gtaggtcgtg
    21421 aaccgcgccg aggaagccga acgttgcctc cgtcttcccg tcgatgacct cgaggggcac
    21481 gccggcctca cgagcgtccg ccagcaggcg atcgccgtcc gtggcttcgc gcacggcgga
    21541 cgtcgccacc gcgatcatgc gctccgctcc cgccccgtcc gcgatcgccc ggaaatccct
    21601 gagcgcctcc acggtccgct cgatcgctgc cggtccgagc cgatcgtcgt cgcgcaactc
    21661 ccgccccagg cgcagagggg cccgggcgtc ctcgaggaca tcgagatgct cacccggacg
    21721 gagacggaac acgatcatcc gcgccgagtt cgaacccaca tccacgacgg cgaaaggacg
    21781 atcgtcggag cgcgagcgtt gcacgaggtg atgctcccac acgcgaccgg agccccgaga
    21841 cgctcgacgg ccctgccgtc gggtcctcgt atgctcctcc gcgacaccat gaccaccctc
    21901 ttcctcatcc gtcacggcct cacggccgtg acgggatcac gtctgtacgg ccgcacggag
    21961 ggcatccacc tggacgagcg cggccgccga caggcggccg cgctcgtcga tcggttcgag
    22021 ggagttcggc tcggcgcgat ctactcgagc ccgctggaac gctgcgtcga gaccctggag
    22081 ccactcgcga cggcccgccg gctcgcgatg cgcgtctctg ctgcgctgat cgagatggac
    22141 gccggcgact ggacgggccg caccctccct tccctccgac gcacgaagct ctgggatacg
    22201 gtgcagcgca atccgtcccg cttccacttc ccgtccggcg agagcttcgt ggacgcagcg
    22261 gcacgcctcc tcgacgagat cgagcggatc gtggcacggc accctcgcgg ccgtgtcgtc
    22321 gtcggtaccc acggggatct cgtcgggatg ctcgtctccc actacgcggg cgcccacctg
    22381 gaccagttcc aacgggtcct cgttgatccc gcctccgtgt ccgtggtgca cctcggcgac
    22441 gggcctcccc gtatcctgct cgtgaacgac acggggagcc ttcgtcggtt cgcagcggcc
    22501 accccgaggg cgacgtacgc cgccgctcgc gccggaaggg gcggtcgtgg ggggaagctg
    22561 cgaggatagg tcgatggagc tgggaccggt cgatcgcatc accgccgacg ccatcggcga
    22621 gccgggagcc cgcatcttct acatccaggc acgatctggc gccgagctcg tcacggtgat
    22681 cgtggagaaa cagcaggtgc agctgctggc cgcttccgtg ctcgagctgt tggaggacgt
    22741 gcccagcgcc gagctcgcga cggagctcgg ggccgggtct gccggaacgc ccgcccccga
    22801 gggatcggcg atggccctcg aggagccgat cgagccgcgc tggcgagccg gccgcctgtc
    22861 gatcggcttc gaccaggatc gcgacctctt cctcctcgag gtcgaggagt tccgacccga
    22921 tcccgaggac ctggaagagg acgatccgcg gtcggtgttg ggagcggacg tgggagcaga
    22981 gccggaatcg atccgcatat gggcgtcgcc gatccagatg ctggcgctct cgcggcacgg
    23041 agcggaggtc gccgcgaagg gccggccgac ctgccagttc tgcggcaacc cgatcgacgc
    23101 ggagggccac gcgtgtcccg cgatgaacgg gcactcgaag cgggactgag cgaccccgcc
    23161 gtccctcgcg tcctggccac cggcgagctc gaggtgctcg gcctgctgcc gaacgcgtcg
    23221 aaccacacgt tcctcgccca ggcaaggagt gccgacgagg agctcctcgc cgtgtacaag
    23281 ccccgccgcg gcgaggcacc gctgtgggac ttcccggagg ggacgctctg tgcccgcgag
    23341 gtggccgcgt acgtcgtctc gcgggccctt ggctggccga acgtacccgc caccgtcctc
    23401 cgtgacgggc cgtacggtgt ggggtcggtg cagcggttca tgccacacga tcccgaggag
    23461 cactacttca ccctcgccga gcggttcccg gaggagctcc gccgcgtcgc cgtgttcgac
    23521 atggtcgtca acaacgccga ccgcaagtcg gggcattgcc tgctcgggga ggacggacgg
    23581 atcttcctgg tcgatcacgg ggtgtgcttc agcgacgagc cgaaactccg gacggtgatc
    23641 tgggacttca tcggcgaccc gctcgaggag gcgacccggg ccgacgtgcg ccgtctcgcc
    23701 ggcgagatcc gcggtggaca ggtgcgcgac gagctcgcca acctcctcgc tccggtggag
    23761 ctcgcggcat tggcggaacg cgcggacacg gtcgccgcgg cggttcggtt cccggagccg
    23821 gggccggatc gcccgttccc gtggccgccg atctagcacc gatggcgcac gagagctcga
    23881 cgctctggcg aaccaccacc gagacaccgc tcgggccgta cacgatcttc ctcagcgatg
    23941 ccggcgtcgt acgcacacac ttcgacgagg agggtggccc cgacgccgac atggacgcag
    24001 tggaggaccg ccggaggacc gcgcccatcc gccgcgaggt cgacgcctac ttccgcggcg
    24061 cgcttcgaac gttcgagacg ccggccgacc tctcggcggt cggagagggt ttcacccggc
    24121 gagtgctgga gatggtccga acgatcccgt acggcgagct ctggacgtac ggcgacgtgg
    24181 cggccgccgc gggcgccgcc cgcgccggcc gcgcggcggg gagcgcgctc gcgcgctccc
    24241 cgatcgagct gttcgtcccg tgccaccgcg tggtccgcgc cggaccgagt ctcggcagct
    24301 acgggcgtca cgacgaccgg cggcggttcc tgctgcagct ggagaaggcg ctccccggcc
    24361 catctctcct gccgtgagaa ggtggaacgg atgaccgttc ggcccgtctc gctcgccacc
    24421 ctcgcgttcc tcctcggggc gatgtcctgc accgccgatc cgacggacgg gtccgacgtg
    24481 gacaccacct tcgccgccca gatggccagc agctggcact acgcgggcgc accccagcgg
    24541 gtccaagtcg gcatcgcggc gagcgatacg aacggcgtcc gcgtggtcac acagggtacg
    24601 gtcgacttca cgttcgccta tctcgggacc gacggatccg gcgccccgga agcgggaccc
    24661 accgtcaccg gcgagtacgt gcccgtgccg ggaacggacg catccggtga ggcgccgacg
    24721 atcatctcgg gggccagcgg cgtctacgag gcagagggcg tcgcgttcga ccgggccggc
    24781 atctggcggg ccacgctgtc gcccgagatc gacggcgtcg cgcgacagct gacggtggac
    24841 ttcccggtga cggagctctc cgacgttcac atccccgctc ccggcgagcc cgccctccac
    24901 accgacacgc tcacgatgga gtcgaaaggg cggaagggcg cgatcgactc gatggcggac
    24961 gtggggggcg agatccccga ccccgagctc caccgatggt cgatcgccga cgcgatcgac
    25021 cgggggcggc ccgcgctcgt gctgttcggg acgcccgcgt actgcgcgag ccggttctgc
    25081 ggtcccgagg tgacggagct gcagcggatc gccgtcgagc accccgatcg aggtgtcttc
    25141 atccatgtcg agatctggaa ggacttcaac gcccagcctc aggtcatcaa cgagggcgcc
    25201 gccgattggc tcctccgcac catgcccgac ggttcgcaga acatgaccga gccctggctg
    25261 tacctgatcg gggccgacgg ggtcatcgtc gatcggtggg ggtcattgtt cgaccccgcg
    25321 gacgtcgaag ctgccctcga ggcgcttcca gagatggagt cctgaccgcc gttccaccgc
    25381 cccacgtacg attggctccg tggaacagac gcccacgatg cccccgccgc ccatcaggga
    25441 acggtgctac cggcaccccg acgtcgagac gggcgtgcat tgcacgcggt gcggcagacc
    25501 catctgcccc gactgcatga tccccgcgcc ggtcggtcat cagtgcccgg agtgcgtggc
    25561 cgaggcgcgg cgcgaatacc ggcggggtcc cggccgccag atcgcggtgg cgaacgtgaa
    25621 gtcgacgtcg gtcacgaagc tggtgctgat cgcgatcgcg atcgggtacg tgtgggagct
    25681 cgtcatcgcg ggaggaccag gctcgctgtt cgagggaccc tccgggcagg ccttgatcga
    25741 cgcaggtgcc ctcgtgccgt actcgggcgg aagcgtcgcg cagcccgtcg gcgggatcgt
    25801 gggaggcgag tggtggcggc tcctcagctc gatcttcctc cacgccggcg tgatccacct
    25861 ggggctgaac gcctacgtcc tgtggatctt cggcaccgcc atcgagcggg acatcggccg
    25921 cgtctccacg ctcgcggtgt tcctcgtcac gggcgtcttc gcgggcgcga cgtccttcgc
    25981 gttcgcctcc gggttcaggg tcgcggtcgg cgcatccggc gcgatcttcg ggctcgtggg
    26041 agccttcgtc gcgtacaact acctccgccg cagtcacgtg atggcgcaag cgaggctccg
    26101 ctccgccctc tcgatgctcc tcatcaacct ggtgatcggc ttcacgatcc ccgtgatcga
    26161 ctggcgggcc cacgtcggag gactcgttgc gggcctcgtc gcgggcttcg cggtcgatcc
    26221 gtctcggccg gcggcgatcc gtcgggtcgc cgccgccagc ggcctggtgg cgttgctcgc
    26281 gatcgcagct gcactcgtcg cctaccggag cgcgcagatc ggcgcggacc cgtcgatcct
    26341 cttctcctct tgacggacgc gctcagggcg tggtctccac gctcccccgc tcggtgagcc
    26401 cgtagaggta accctggcgc gtgccgaggt agatccagcc acgccacacc gccggcgtcg
    26461 actcgatgca gtcgctgaag tgcagcttcc agagcagcac cgggtccgct tcgggctccg
    26521 acacgtccca tgcccacaga tcccccgcgc aatcgccctg gatcagcacg ccgtccacga
    26581 cgaccggcga cccgatcgcc ggtgcgccga tccgtcgctc ccagcggatg tcgccggtat
    26641 cccgctcaac ggccagcacg cggccggccg cagtggtgaa gtacacgaga tcaccgtaga
    26701 gggccggcgt cgaccagcta cccccggcac cctcgaagcc gatctcccgg gcgtcgatcg
    26761 accacacgag ggggtcgctc ggtcgcgacg ggctgagctt catcagctgg cccacctcgc
    26821 tgctccgctc cccggatcgc tgatattcgc tggccacgta caggtcgccg acgtcatcga
    26881 tcacgaccgt ggcatcggtg tcgtccccgg tccagaaccg gaagacccgc tcgagatcgg
    26941 agccgccggc cagcacgtcg gagacgtccc aaccctgcac caaaccaccc gagttcgcga
    27001 agtacaggat cccttcgtgg agggagaccg agttctcgat cgagacctcg gtgtcgccga
    27061 gcgctcggag gagctcgtcg tcgaaccccg ggacggtcgc cacgacctcg ggatcgaccg
    27121 tgacgagccc gagctcgtcg tagctccgat gcagacggat cacgtagagc cacccgttct
    27181 cgccgccctc gaacaagacg tcgtccacga ccagcgcggc gccgtcccaa tcgtcgttgc
    27241 ggagcggccg ctcgacactc gtaacgccgt cgatcgacca gagcacgagg ggctcgccgc
    27301 gagcgtggcg atcggtcgcc acgatccgga accggccgtc acgggagccc gcgtagtaga
    27361 gcgggaaccc gtccgggtcc gtcgtcgccg agcccttcgc gaggtcgcgg gtggcgagcg
    27421 gctgccgcag cggcagaccc gtcagcccgt ccaggaagtg atacatgccg tcgtaggcgc
    27481 cctcgcggac ctcgatcgag ccgtcctcac ccaccaggac gttcggctgc cccgtccagc
    27541 cggtcccgca ccactcgctc cgatcgatgc ccacgaacga caccgagcag agccgggtcg
    27601 agggagcacg ccacccgatc accgggtcca gcggcacggg cccctcgccg taatagctgc
    27661 gcgccgcgtt gccccggaac gtgaccagac cctcgaacgc cgtgttgatg gggtcagagc
    27721 cctccggcgg ccgatcgttc ctgagcgggc caccgcggtg ctgctcgtcc tcgatccggg
    27781 acaggatgaa cgcgatcaac aggacggcga ccccgatcac gagggcgccc gacacgatcg
    27841 gaaggacggg gaacctcttc gtcaccggcg cccgggacgt cgcacgactc gcacgggcca
    27901 ccgacccatg ctatgccgtc agacgggttc tcccgcggag cggtaggagc ccaggatctt
    27961 cagcagggac gtgtgctccc ggacctcctt gagggcggac tccacctcgg gatcgtcggc
    28021 cgccgccatg aagtccacgt acatcaggta ctcgaaggct gccccgtgcc tcggtcgcga
    28081 ctccagcttg tggaggttga tcccacgcgc cgcgaacggc tccagcgctc cgagcagcga
    28141 ccccggctcg tcgagcaccg ccatcaccat cgacgtcttg tccgcgcggc cgaaccctgg
    28201 atcggtcccg ccgatcaccg cgaacttcgt gaagttctcc ttctcggtct ggatgcgctc
    28261 cgcgagcacg gtcaggccgg cgagcccggc ggcatccacg ctcgcgatcg cggcgtccgc
    28321 ggggtcgttc gtctccgcga tcttccgggc ggcacccgcg gtgtcgtgct ccggaaccgg
    28381 ttcgatgcgc atggaggtca ggaagtcctc gcactgcgcg agggcctgcc aatgcgagtg
    28441 cgcgcggcgc acgtcttcca ggcgggtgcc cggaacgccg agcagcgcat ggtcgacccg
    28501 cacgatcgac tcccccacga tcctgagcag gctggtgttg gagagcagct cgtaggtctc
    28561 gttcacggat cccgcctgcg agttctccag gggaacgacg ccgaaggacg cctcgccgct
    28621 cgtgacccgg gagaacgcgt gtcgcaccgt gtcgcacggg agaggctcgg cgtccgcgaa
    28681 cagcgagcgg acggcccgtt cgctgtacgc gccgggctcc ccttgatagg cgaccttcat
    28741 gtcgccggag gctagcgggt cggcgggcga gccgacagcc ggctcatgcg ttgatgaact
    28801 gttcccagat ctggatcgcg cccggcccga cgatgacgac gaacagggca ggcaggatgc
    28861 ataggatcag cgggaacacg atcttcacgg gtgtctgctg ggccttcgcc tcggccgcct
    28921 ggcgacgctt gatccgcagc tgctgcgcct gggtccgcag gacgctcgcg atcgagacac
    28981 cgaagacgtc ggcctggatc atcgcgagtg cgaacgcgtt cagctcctcg acgtcggtcc
    29041 gctcggcgag gttgcggaac gcgtcgcctc gcggcactcc cagctggatc tcctggagca
    29101 tccgcgcgaa ctcggaggag aggatgccgg gcacgttctg cacgacctgt gcgatcgcgg
    29161 cgttcagcga gagccccgcc tccacgctga tcgtcaggag gtcgagggtg tccgagagcg
    29221 tgcggaggat ctccttctgc ctctcgtcga cacgccggtt caacatggag tcgggcgcaa
    29281 cgaacccgac gaatcccagc aacccgaaca gaacgatccg gatgaacggg gtgacgtccc
    29341 cgatgacgga ggacacgacc aacgccagga cgaccccgga gaccagtccg atgatcttga
    29401 acgcgaggac gcgctccgcg tcccatcctg tgggcgtccc tgccaacaag agcttgcggg
    29461 cgaggcgatc tcgggcatcg atcggtgtga tgcgtcgagc gaccctcgtg gcgccggcga
    29521 ccaccggcac gatgatccgc gccccgaagc tctcggagag gtgttgctcc ctgaggttca
    29581 cgttctcgct cgacgtcccc gcgacctgcg attcgagcat ccgaacggcg cgacggcgct
    29641 cggactggct caggtcgaag aagatgccga agagaaggat ggctccggcg atgcatccga
    29701 tagcgagtac cagccagacc tcagacatcg atatccacga tctttctcat ccagaggatc
    29761 cc
//
LOCUS       DASUYD010000005         8663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_884400, whole genome shotgun sequence.
ACCESSION   DASUYD010000005 DASUYD010000000
VERSION     DASUYD010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884400"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(597..1553)
                     /locus_tag="VF195_00235"
     CDS             complement(597..1553)
                     /locus_tag="VF195_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579295.1"
                     /translation="MKSAKVVVGVAVLMMSMAAAAAAGPHLSSWTTAQKIDEIAGNNS
                     ELNTAFVDGCPIQSPDGLDLYLASNRPRFPGDTRTDLDIWVAHRASTDAPFGPPVNLG
                     EPVNSLADDFCPTPVRGHGLFFVSREALPGACGLGDIYFSRRDPSGAWAEPVHLACAP
                     EGPNGPLDEQGPSYVELDGDAFLFFSSSSAAVPGDIYVSERLANGSFGPASPVVELNS
                     AGNDIQPNVRKDGLEIVFSSNHAYPGAQGLQDVYVSTRDGIEDPWSTPVNLGTSVNTA
                     ASETRPSLSWKALTLLFGRAPGPEGSSDIYVSTRDKLAGSDL"
     gene            complement(1677..2492)
                     /locus_tag="VF195_00240"
     CDS             complement(1677..2492)
                     /locus_tag="VF195_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579296.1"
                     /translation="MSELREVFEMVTKQTEPDVDAWNEQERRQRRASRNRKFGALAIA
                     AAIGIVAVVVVIRAADEGTGTQPGGQPTPTAAQPIPSLPGGALEPGRYVFTSYDPGLD
                     ASYRISIDVADGYEGIGEWAARRIGTTQTGVNTLAIGDVYADPCQWEGSLLDRSAIST
                     TDEVVVALASQKGLRVSTPTDVTVDGFAGTYLERRVPARTNLSDCDGGQFRVYLTPRG
                     GERYLVSGLLQQLWILDVDGVPLVVEAGFDAGTSAQVRTELLRMVESIRIDPV"
     gene            complement(2485..3003)
                     /locus_tag="VF195_00245"
     CDS             complement(2485..3003)
                     /locus_tag="VF195_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579297.1"
                     /translation="MQAEGDVIRFPEAAVDAPHDFAAFFAEEHRGLFKALYFVTGNRA
                     DAAELMQDAFLKLWERWDRIDRIADPRGYLFRVALNGFRMRSRAAIRATRSLVPVASS
                     TDPFDEIDLRDDVRRMLLELSPRQRGALVLLDMYGYGSEEAARIMGIRPSTVRALATQ
                     GRAVLRKGGPHV"
     gene            4692..5675
                     /locus_tag="VF195_00250"
     CDS             4692..5675
                     /locus_tag="VF195_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEX6579298.1"
                     /translation="MVRAERNEYRANALDERDHQVLLLLLEHKVLTTYQITSVCFRSL
                     RRCQHRMRELKEMGLVSSFSVGRGFGEGRAPACWHLTKEGLVEIADAKDVRTSDLPWV
                     PDSSYRSSKLLAHRLGVNAYFCALAEASRVLQGHCLATWRPEHWVRTRAAEIKPDGFG
                     RYLHPGGACEFYLEYDRATEASGALSRKLEGYLRLAAGWTREQELSGFPNLLVLVPEE
                     EREEKVASAFRSAVAGHHVSGSLTSSFPLYVASEGPLTDLGVLGPVWKHLLTDGERLS
                     LVDLPARPGDLYRATRCLGRYFTDADPSHRGRISPASTTPRFRALPPRHAP"
     gene            complement(6457..6972)
                     /locus_tag="VF195_00255"
     CDS             complement(6457..6972)
                     /locus_tag="VF195_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579299.1"
                     /translation="MPLALGDLGPENSLQAAPERRRDGDAFIEGLDLSLEVGGLDVPA
                     LRASRATGVPSDAAEVAKPTLGDCVVQARAALRADQRALQVVVVLPAPLPACGVRVKH
                     VLHLVEQLTEDERLVDAFALHAPPADDADVHGIPEHPVHRALPRWIPQPIPQALLSQG
                     IGQRVQGQRAG"
     gene            complement(7355..7430)
                     /locus_tag="VF195_00260"
     tRNA            complement(7355..7430)
                     /locus_tag="VF195_00260"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7395..7397),aa:Ala,seq:ggc)
     gene            complement(7441..7839)
                     /gene="rsfS"
                     /locus_tag="VF195_00265"
     CDS             complement(7441..7839)
                     /gene="rsfS"
                     /locus_tag="VF195_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018161268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6579300.1"
                     /translation="MPGSDANHAGSREIAVTAARAAAAKQAVDVTILDVHDLIVITDY
                     FVICSGQTDRQIKTLVEEVERAVREIGEKPVRREGDADSRWVLLDYIDVVVHVFAQEE
                     REYYDLERLWRDAPRLRWNATDQAAAGSLD"
     gene            complement(7890..8114)
                     /locus_tag="VF195_00270"
     CDS             complement(7890..8114)
                     /locus_tag="VF195_00270"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX6579301.1"
                     /translation="MLNGNGVPGTGEAVAERIIPGGFRVAVSQNASDFDHPETPILVA
                     SPEDVALAERVRDLLGVGSVSVSADRVSLQ"
     gene            complement(8292..>8663)
                     /gene="nadD"
                     /locus_tag="VF195_00275"
     CDS             complement(8292..>8663)
                     /gene="nadD"
                     /locus_tag="VF195_00275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003976224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6579302.1"
                     /translation="RVEIDRDGPTYTVDTLRMLGTEYPDAELYFITGADAMLEILDWK
                     DPEEVLSSAHFIAASRPGYDLTRFEKAGPARDAGVSMMSIPALAISSTDVRERIHDGR
                     PIRYLVPEGVKSYIEKAALYR"
BASE COUNT         1424 a         2991 c         2903 g         1345 t
ORIGIN      
        1 cgcgcagtgc tctcaagttg tcgagtcact gccgccgccg gcctcgagct ggacgatgtg
       61 atcgaccctg ggagctctgc cgacgcagat gtcggcgtag cgcagctgaa gcccaaggca
      121 gcattccgca acttgtggac ctggcaggac gatgtgtgaa ccgcctttcg ctcctccagg
      181 ccctccggaa tcaaacggcc gcataggatt cacgtccatg gcatttgccg gcattcagct
      241 gagcggtgct tcgagtggtc agccgagcgg ttactcgggc ggtcacatcc cctcgccagg
      301 tgccaacggc ggcgcgtcaa tacgcctatc ttggtctgag tcccacgccg gacaagagca
      361 tttgtgatcc gccggacccc gagagatagg gagtctgcag gaatcggctc gggactgtgc
      421 gatttcgagg agatcgagcc ggaactattc cgcttcccgg cgatcaaccc ggcgaacctc
      481 cgccgaaggg tggaggaggc gttcgccagc ggcatctaga gccgaaggcc cggcaaccat
      541 ccggctgccg ggccattcgg tgttcgagct ctaccgcttc gacgtcgggg tgcctctcaa
      601 aggtccgagc ccgcgagctt gtcgcgggtg ctgacgtaga tgtcggatga cccctctggt
      661 ccgggggcgc ggccgaacag gagcgtcagt gccttccagg acagggaggg ccgcgtctcg
      721 gacgctgcgg tgttcacgga cgtgcccagg ttgaccggcg tcgaccaggg atcctcgatg
      781 ccgtctcggg tggagacgta cacgtcctgc aggccctggg cgccggggta ggcgtgattc
      841 gaggagaaga cgatctcgag cccgtccttg cgcacgttcg gctggatgtc gttgcccgcg
      901 ctgttcagct cgacgacggg ggaagcggga ccgaagctcc cgttcgccaa cctctcgctg
      961 acgtagatgt ctccaggcac ggccgccgag ctgctggaga agaacaggaa ggcatcccca
     1021 tcgagctcga cgtacgaggg gccctgctcg tcgagcggac cgttcgggcc ctccggcgcg
     1081 caggcgagat ggacgggctc ggcccaagca cccgagggat cgcggcggct gaagtagatg
     1141 tcgcccagac cgcaggcccc gggcaacgcc tcccggctga cgaagaacaa accgtgtcca
     1201 cgcactggcg tggggcagaa gtcgtcggcc agagagttga ccggctcgcc caggttcacg
     1261 ggcggaccga acggggcatc ggtgctcgcc ctgtgggcaa cccagatgtc cagatccgtc
     1321 cgcgtgtcgc cggggaagcg tggacgattg gacgccaggt acaggtcgag accgtccgga
     1381 gactggatcg gacagccgtc cacgaaggcc gtgttcagtt cggagttgtt gccggcgatc
     1441 tcgtcgatct tctgcgccgt cgtccaagag gacaggtgcg gtcccgccgc cgccgctgcc
     1501 gccatcgaca tcatcaggac ggcgaccccg acgacgacct tcgcgctctt catcgtgacc
     1561 tcctgtctcc atgaccggac gggtcgtgcc tcgcgatcca gagtcatcag cctcctcctt
     1621 ctatcagtgg tgggtgtagg tgtggtcggg cgggagcgag tgcgcccccg cgccctctag
     1681 acaggatcga tccgtatgga ctccaccatc cgcagcagct ccgttcgcac ctgcgccgac
     1741 gttcctgcgt cgaacccggc ctcgaccacg agcggaacgc cgtcgacgtc gaggatccac
     1801 agctgctgga gcagaccgga cacgaggtac cgctctcccc cacgcggggt cagatacacc
     1861 cggaactggc ccccgtcgca atcggaaagg ttcgtacggg cgggcaccct tcgctccagg
     1921 tacgtgccgg cgaagccgtc gaccgtgacg tcggtcggtg tcgacacgcg gagccccttc
     1981 tggctcgcga gcgccaccac aacctcatcg gtcgtcgaga ttgccgatcg gtcgagcagc
     2041 gatccctccc actggcaggg atcggcgtag acatcgccga tggcgagggt gttcactccg
     2101 gtttgggtcg tcccgatcct gcgggcggcc cattcaccga tgccctcata tccgtccgca
     2161 acgtcgatcg agatccggta cgaggcatcg aggccgggat cgtaggacgt gaagacgtac
     2221 ctcccgggct ccaatgcccc gccgggcagc gaagggatcg gctgtgccgc cgtgggagtc
     2281 ggctgccctc cgggctgtgt cccggtccct tcgtccgccg cgcggatcac gacgacgacc
     2341 gccacgatgc cgatcgcggc ggcgatcgcc agggcgccga acttccggtt ccggctcgcg
     2401 cggcgctgac gccgctcctg ctcattccaa gcgtcgacat caggttctgt ctgtttggtg
     2461 accatctcga agacctccct cagctcagac atgcgggcct ccctttcgga gcacggcgcg
     2521 gccctgtgtc gcgagcgcgc ggacggtcga cggacggatg cccatgatcc gtgcggcctc
     2581 ctcggagccg tagccgtaca tgtcgagcag gacgagggcg ccgcgttggc gcggcgagag
     2641 ctcgagcagc atgcgacgga cgtcgtcccg caggtcgatc tcgtcgaacg ggtcggtcga
     2701 cgaggcgacc ggcaccaggc tccgcgtggc ccggatcgcg gcgcgcgacc gcatccgaaa
     2761 cccgttcagc gccacgcgga acaggtaccc cctgggatcg gctatccggt cgatccggtc
     2821 ccaccgctcc cagagcttca ggaaggcgtc ctgcatcagc tcggccgcat cggcccggtt
     2881 ccccgtgacg aagtacaggg ccttgaacag accgcggtgc tcctcggcga agaacgccgc
     2941 gaagtcgtgc ggagcgtcca cggccgcctc cgggaacctg atcacgtcgc cctctgcctg
     3001 catcgccctt ctagatgccg ccggagcgtc tcgcgcttag ggggaggctc aggaatggcc
     3061 cttctcggga gcgtgcgcta gtccgccgat ccgccagagg cggttccgtg ccgatcgatc
     3121 gtacgagcga tccgccggtc ggtcacccgg tcgggcgacc tgcggggcac gtcctcgaag
     3181 ccgccgttgg tgaacgatgg gaagctcgcg agcaggcgga gccagggaca gaaccggccc
     3241 gctcagtcgg ggggtcaatc cggggttcat tcgggggcca aatccgtggc gcagtcgagc
     3301 tccgctcagt gctcgccggc tgaccctggc tccaggagtc cccccccgtc ccctctccct
     3361 cccgcttcga ctgctgcgac ctgcacctcc ggagaccccc caagactcct cggccgacgc
     3421 cgtcacgcta cggcgcctcc ccctcgccct tgtacgggtg aacatggtcg tgctcatcac
     3481 accggtcccg ggcaacgggg gcacggttgg ggcagcctgc ccggtcgcag ctgtcgccga
     3541 ttcctgagca gttcccatag ttccggtcgg agtccgcgtc gtcgtctcca cgcgaggatc
     3601 cggtccacca cgggcaggct cgaacctcgc gtgagcggcc gggcggaacc gtgtttggac
     3661 ccgaccgagg gttcggtcca cagagttccc gagaagttcc ctgtcctcgc tgtcaaggtt
     3721 cgggccctcg catgtctttg tgccggtgat gatgtccgac cgccgaactc cacccgggcc
     3781 gcatgttggc accctggctg gcccagggct cgtccgccaa gacgacgacc acatcgagtc
     3841 acgacgctgg cccgatcgcg tgcgcggcgc gtgcttcggg gccgacgccc cgtccctatc
     3901 ggaagacccc ctgtcccctt caccggctac gggcctcctc gtctctacgc catctgggcc
     3961 gagacgatcc caggcgtggt gcctgtcccc acccggtccc ccgccctggc gagactccgc
     4021 gtcctctcta cgcgtctggg ggactgtccc cgtccaactc caggcacaag gcgtggagcg
     4081 aacgtggagt cgggagggac tgaatgggga ccggcagcac tcaacatcga gcagacaaca
     4141 gcagacgtat cggcgatcca ccgcttggcc gcagcaggct ccgagggcga cggctgtctc
     4201 gccacgggca acccgactcg ggcaccgagc ggagctccgc gggaaggccg gcgccacggt
     4261 cagtgacgcg agccggacga gcgatcagga cggccccaaa cccgggcacc tcacgaggag
     4321 gcgcgctcgg agcgagcgga ggccggagga ccgctcggac ggcgagcgaa cgggaccgga
     4381 gcgccggcga ggtccgcggt gatccgcgag ggagccctga accggatggc cgaaggcgag
     4441 tctcgccggc gacctgagtg cgccatgcga gcggtgagaa aggaggtgag cacgggcacg
     4501 atcagaagat ccgtcgacca ggcggtggtg cgcgcacgcg ccctggcgcg gcgtcgcgtt
     4561 gccccgctgt cgtgagtgac ggcgcgtagg cggcgcgacg gcgccgggcg cgaaagcgca
     4621 aaaccgctgg tcacggagcc tctacgccga aggccacgaa ccgaaggagg ggggagggct
     4681 gtgcccgaat catggttcga gcagaacgaa acgaatatcg cgcgaacgcg ctcgacgagc
     4741 gagaccatca ggtcttgctc ctcctgctcg agcacaaggt cctcaccacc taccagatca
     4801 cgagcgtctg cttcaggtcc ctcagacggt gccagcaccg gatgagagag ctgaaggaga
     4861 tggggttggt ctcatccttc tcggtgggga ggggtttcgg ggaggggagg gctcccgcgt
     4921 gctggcacct cacgaaggaa ggcctcgtcg aaatcgccga cgcgaaggac gtccggacct
     4981 cggacctgcc ctgggtgcct gattcgagct atcgatccag caagctcctc gcccaccggt
     5041 tgggagtcaa cgcgtacttc tgcgcgctcg ccgaggcctc cagggtcctc caggggcact
     5101 gtcttgcgac ctggcggccc gagcactggg tccggaccag ggctgccgag atcaagcccg
     5161 acgggttcgg gcggtacctg caccctggcg gggcatgcga gttctacctg gagtacgacc
     5221 gggccaccga ggcctccggc gcgctgtcgc gcaagctgga gggctacctg aggctcgcgg
     5281 cgggatggac cagggagcag gagctcagcg ggttcccgaa cctgctcgtc ctcgttccgg
     5341 aggaagagcg cgaggaaaag gtcgcatccg cgttccgctc ggctgttgca ggccatcacg
     5401 tctcgggctc actcacgagc tcgttcccgc tctacgtggc gagcgagggc ccgctaacgg
     5461 atctcggcgt cctcgggccg gtctggaaac acctgctcac cgacggcgaa cgcctgtcgc
     5521 tcgtggatct tccagcccgg cccggcgacc tctaccgagc tacacggtgt ctggggcgct
     5581 acttcaccga cgccgatccc agccaccggg gccgcatctc ccccgcctcc accacgcccc
     5641 gattccgcgc cctgccgccc cgacatgccc cttgagcccg cggcgcggag acgcgccgac
     5701 cgaagccgaa ctccctcacc cgcgcacgag ggacggcgag gccacggagc agggacgtga
     5761 attgcagcgg gacgggctcc acgccagccg ggagccagtg tggacagagg acgatgctag
     5821 cctcaggcct ctcgcgaggg cactgctcgc gctcgccgag cagctgagcc aagaggaggg
     5881 accgtgaagg ccgtcgtcta cctccgcgtc tcgaccaagg agcaggccga ggagggctac
     5941 tcgatccccg cccaggccga ggcgtgccgc cggttcatcg ccgacaaggg ctgggagctc
     6001 gccgacgagt acgtcgaccg cggtgagtcg gcccgtaccg ccgaccggcc ccagctccaa
     6061 gcgatgctcg cccgcctgaa cgaggatcca tcaatcgact gcctggtggt tcacaagctc
     6121 gaccgcctcg cccgtaatct ggaagaccac gccgcggtcc gcgcagccct ccgcaaggcg
     6181 catgtgcagc tgcactcggt gaccgagacc cttgaggact cggcctcggg caagctcgtg
     6241 gaggggatcc tcgcttcgat cgcggagttc tactccgcca acctcggcca ggagatccgc
     6301 aagggcatgg accagaaggc cgcccagggc ggttggccga tccgggcacc gttcgggtac
     6361 cggaacgtcc gtcgcgatgg ccctgggcgc cgaggtgagt cggtgctgga gcccgatcag
     6421 caggcgccgc tcgtcgtgtg ggccttcgag cgctactcaa ccggctcgct gtccctggac
     6481 gcgctgaccg attccttggc tgagaagggc ctgcggaatc ggctggggaa tccaccgggg
     6541 aagagcgcgg tgcaccggat gctccggaat cccgtgtacg tcggcgtcgt ccgctggagg
     6601 ggcgtggagc gcgaaggcat ccaccagccg ctcgtcttcc gtgagctgtt cgacaaggtg
     6661 cagaacgtgc ttgacgcgca ctccacaggc ggggagcgga gctggaagca cgaccactac
     6721 ctgaagggca cgctggtctg cgcggagtgc ggctcgagcc tgtactacgc agtcgccaag
     6781 ggtcggtttg gctacttccg ctgcatcgga cggaacaccg gtcgcacgcg atgctcgcag
     6841 ggccggtacg tccaggccgc cgacctcgag cgagaggtcg aggccctcta tgaaggcgtc
     6901 tccgtccctg cggcgctcag gcgccgcctg gagcgagttc tccggaccga ggtcgccgag
     6961 cgcgagcggc atcgggctga ggcgacgcag ttcttgggca gccgcctcag ccagcttgcc
     7021 agtgagcgag agaagcttct ccgcgcctac tacgccgacg cgatcgacgt gaccaccctc
     7081 aaacgggagc aggcccggat caacgccgaa gtggcacagg ccgagtcaca gctcgcgaac
     7141 gacggcgaga agctggcgca tgcgaaacag atcatcgacc tcgcactcga tctggcaaag
     7201 gactgcgccg cgagctatcg cgaggccagg cccgaggtcc gcaagatgtg gaaccgcgcc
     7261 ttcttcaaca cgatccgcat tcgggacggc gcgatcgccg acttcacgca cgaggagccc
     7321 ttcgcgtctc tgctcggttc acacaagggc tcaatggtgg acccaagggg attcgaaccc
     7381 ctgaccttct ggctgccagc cagacgctct accagctgag ctatgggccc gcgggagcgg
     7441 tcagtctagc gaaccggccg cggcctggtc cgtagcgttc caacgcaggc gcggcgcatc
     7501 gcgccagagg cgctcgaggt cgtagtactc gcgctcttcc tgggcgaaca catggacgac
     7561 gacgtcgatg tagtcgagca gcacccaccg ggagtcggcg tcgccctcgc gccggaccgg
     7621 cttctcgccg atctcgcgga cggccctctc gacctcctcg accaacgtct tgatctgccg
     7681 gtcggtctgg cccgagcaga tcacgaagta gtcggtgatc acgatcaggt cgtgcacgtc
     7741 caagatcgtg acgtccaccg cctgcttggc ggccgcggcc cgcgccgccg tgacggcgat
     7801 ctcgcggctc cccgcgtggt tggcgtcgct acccggcacg ccgtcgttcg ctcgtggtca
     7861 cctggagatg tccttcccga cgacgatcgt cactggagcg atacccgatc cgccgacacg
     7921 gacacgcttc ccacaccgag caagtctcgc acacgttcgg ccagagcgac gtcctcgggc
     7981 gaggcgacca agatcggcgt ctcgggatgg tcgaagtccg aggcgttctg cgagaccgcc
     8041 acgcggaacc cgccagggat gatccgttcg gccaccgcct ccccggtccc agggacgccg
     8101 ttgccgttga gcacgttgac cgggaccgcc tcggtggccg gcccaccgaa cgcctcgacc
     8161 agggccctcg gtcactgatc gggcccacgc cacgtccgcc gacaacagcg cctctcgcgc
     8221 gcgcgaccag gagcgccccc gagccggcgg tcgcgagaac cgcgacgagc acagcaggtt
     8281 cagccgccgt gtcaccggta gagcgccgct ttctcgatgt agctcttcac gccctcggga
     8341 acgaggtagc ggatcggacg cccgtcgtgg atccgctcgc ggacgtcggt cgagctgatc
     8401 gccagcgcgg ggatgctcat catgctgacg cccgcgtcgc gcgcgggacc ggccttttcg
     8461 aaccgcgtca ggtcataccc tggacgggac gccgcgatga aatgcgcgct cgacaggacc
     8521 tcctcgggat ccttccagtc caagatctcg agcatcgcgt cggcgcccgt gatgaagtag
     8581 agctcggcgt cgggatactc cgttccgagc atgcgcagcg tgtcgaccgt gtacgtcggc
     8641 ccgtcgcggt cgatctccac gcg
//
LOCUS       DASUYD010000006        13232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1106309, whole genome shotgun sequence.
ACCESSION   DASUYD010000006 DASUYD010000000
VERSION     DASUYD010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106309"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..718)
                     /locus_tag="VF195_00280"
     CDS             complement(5..718)
                     /locus_tag="VF195_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579303.1"
                     /translation="MSRGTPPPRELTISPELHEPHVRRTHVPNVLGGLYGSEPAPRPI
                     RDRHTPDELTPPHRDLIDQFDLPEAVRARIRDPDDPIDLVRSILEAAGVHCEIDGDLV
                     RAGESALVIMRTSGDVVIGHEALNHAYLRFSRSGAQRGVVIALGFMDPREVKRREMLA
                     PQVLHAGPDAIQRMADAVSLGADPLRFAVAIPVSPGRSHVSPERTRRASRSDRSTGHL
                     SPSGIPKPAGDPESGEVRA"
     gene            1220..1930
                     /locus_tag="VF195_00285"
     CDS             1220..1930
                     /locus_tag="VF195_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007499322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6579304.1"
                     /translation="MKRVRDVMTTEVVVVGDRTPFKEIVGLMRQHDVSALPVVDATDE
                     LVGIVSEADLLMKEELVAEGGSHRRFQSRQRRIDRAKASGVVAQELMSSPVETIDPEA
                     PLARAARLLHDRRVKRLPVVDANGKVVGIVSRADLLKVFLRPDREIRDEIEDTLAKVA
                     FEPGPVRVTVVDGAATLQGQVELRSQIPVIVGIARAVDGVVDVAPRLSHEVDDVTPSL
                     ELLTPWGPYTPTGPPHRE"
     gene            complement(2101..3840)
                     /locus_tag="VF195_00290"
     CDS             complement(2101..3840)
                     /locus_tag="VF195_00290"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX6579305.1"
                     /translation="MSPAAERARAAEHAWVEALRTGSAPVPVPSRGHELLEQALLNKD
                     TAFSEHEREVFGLRGLLPPRVRTLEEQVALEIGKNRRKGDELERFIGLAALQDRNETL
                     FSRLLLDNLEELMPIVYTPVVGRACQEYSHIMRRPRGLWLTPEDVDRIPELLSNARHE
                     DVRLIVATDNERVLGLGDQGAGGMGIPVGKLALYTAGAGIHPALTLPVSLDVGTDNEA
                     LLNDPGYFGHREPRLRGPAYDGFIEAFVEGVLEVFPHSVLQWEDFKQHNALRLLDRYR
                     HRITSFNDDIQGTAAVVMAGIRSALRILHEPLGAQRFVFLGAGAAGIGIARLLRAQML
                     REGVLEGDVRRAIVQLDSRGLTYAGREPLDADKEAFALGGDEMAAFRFVTGETYGLEE
                     VVKHVHPTFLIGTSGTPGSFSEVTVTEMAAHARLPVILPLSNPTSRTEAQPRDILEWT
                     RGRAIVATGSPFPPVDRGGRTHVIGQANNAFCFPGVGLGALVAEAREVTDEMFLVAAE
                     ALARVVSEARLAEGSLYPSPSDLRVASRAIAVAVARMARDAGVGRQFREDELGPAVDE
                     MMWEPRYLPYEPV"
     gene            complement(3837..5228)
                     /locus_tag="VF195_00295"
     CDS             complement(3837..5228)
                     /locus_tag="VF195_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX6579306.1"
                     /translation="MNDTLIPLAYLFAVACFVLALKWLSHPTTARRGVRTGEIGMLVA
                     VVATLVDLRIVEYRWLGVAIVAGVALGVPLGLLVPMTAVPQRTALSHAFGALAAGLVG
                     TAEYYLRRPEIDGTTMVAIGLEVVLGFLTVTGSLIAAGKLQGTLPDHPWVYRGQNLVN
                     VSVLGVALVLVGILVIDPGRAVLFPIVIVVSLVFGTLLVTRIGGADMPTVISLLNSFA
                     GLAASMMGFVLDNELLIVAGALDGSSGLILSITMCKAMNRSFANVLFGGFGAETATGD
                     VEQRPFRSIEAEEAAFLIDAARSVVVVPGYGMAAAQAQHKVRELTDALQHRGVDVKFA
                     IHPVAGRMPGHMNVLLAEADVPYDQLFDLEQINGEFPQTDVVLVVGANDVVNPAARSD
                     TTSPIYGMPILDVDAASSIIVVKRSMRSGFAGVDNDLFYLDKTQMLFGDAKAVIGQVL
                     DELSRLPAKEAAR"
     gene            complement(5237..5554)
                     /locus_tag="VF195_00300"
     CDS             complement(5237..5554)
                     /locus_tag="VF195_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015209093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX6579307.1"
                     /translation="MSQALEVGLFIFVIASFLGYTVISRVPGLLHTPLMSLTNALSAI
                     SIVGSLILAGARESEFATVLASVAVFAATTNIVSGFLITDRMLKMFRRREAPEVPKEG
                     TSS"
     gene            complement(5538..6713)
                     /locus_tag="VF195_00305"
     CDS             complement(5538..6713)
                     /locus_tag="VF195_00305"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX6579308.1"
                     /translation="MERLVVGVPTETFPGEARVALVPSVVRRLSDAGIDVVVEHDAGE
                     RAGFPDESYAAHGARIGARAEVFGASIIAQVRACGTNPKNERDDVDRLRPGHVVLGIS
                     DPLGSPEGVRQAADRRIISFALDLMPRITRAQSMDVLSSQATVIGYKAVLLAANRLPK
                     MFPMLTTAAGTVPPAQVLVIGAGVAGLTAIATARRMGAVVQGYDVRPSAQEDIESLGA
                     RCVLLPLQPGDAEDPAGYAKEFGAAFYQRQQELIAQVVAGVDVVITTALIPGKRAPVL
                     ITEQAVLGMPAGSVIVDCAAERGGNCELTDPDNEVVTENSVTILGPTNLASTVPHEAS
                     VMYAKNVVAFLLHIVRQGELAIDLEDEVIAGTLVTREGEIVNPRVVGSLEVGSVPSA"
     gene            complement(6716..7753)
                     /locus_tag="VF195_00310"
     CDS             complement(6716..7753)
                     /locus_tag="VF195_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012629726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX6579309.1"
                     /translation="MSVDLRTTYLGLELANPLVPSASTLSSRIDNLKRLQEAGASAIV
                     MQSLFEEQIEHEELQTHRVLENGAESFPEALSYIPEMEEYNTGPDEYLRHLAACKREL
                     EIPVIGSLNGASEGGWIRYARLIQDAGADALELNVYFIAADPDESGSQVERRYLDLVS
                     AVRGSVSIPLAVKVGPYFSSMANMALQLEAAGANGLVLFNRFLQPDIDLDTLRVDPTL
                     HLSTSDELRLPLRWIAMLHGKVACSLAATSGVWTADDAVKLLLAGADVTMMASALFRH
                     GPEYLGVVLEGMRTWLDEHEYESVEQLKGSVSQANVADPSAFGRSNYMQMLVNFTSPY
                     DWREIPGSVQT"
     gene            complement(7750..11373)
                     /gene="nifJ"
                     /locus_tag="VF195_00315"
     CDS             complement(7750..11373)
                     /gene="nifJ"
                     /locus_tag="VF195_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX6579310.1"
                     /translation="MAEGRSMIAVDGNEAAARIAHRLSEVIAIYPITPSTSMGELADA
                     WSAAGRTNLWGAIPQVTEMQSEGGAAGALHGALQGGALCTTFTASQGLLLMIPNMYKI
                     AGELTPTVIHVAARTLATHALSIFGDQSDVMAVRQAGWAILASSDVQAAQDLAAIAHT
                     ATLECRVPFLHFFDGFRTSHEVNVIAELGDEALRELVDDHMVRAHRERALSPDHPVLR
                     GTAQNPDVFFQAREASNPYYAIIPQVVQQTMDRFAELTGRAYHLFDYHGVADAERVVV
                     LMGSGEGAARETVDELVARGEKVGILNVRLFRPFSAEAFLGALPTTVRAIAVLDRTKE
                     PGAPGEPLYQDVVTVLAERIVSGAISTMPTVVGGRYGLASKEFDPAMAKAVFDELAKD
                     RPKNHFTVGIVDDVSHTSLEVDRSFSTEADDVVRAVFYGLGADGTVSANKSSVKIIGE
                     GTDLFAQGYFVYDSKKSGSVTTSHLRFGPRPIHSTYLIGSGQASFVACHQFGFLERLD
                     VLDVAARGATLLLNCPFDPGSVWEHLPVEAQQAILEKELRLYVVDAQKVATAAGLGLR
                     VNTILQTCFFALANVLDQAEAVARIKVAIEKNYGKRGRAVVEQNFAAVDAALNGLHEV
                     PVPAVIGGDVHRSLPVPEDAPDFVQRVTAMMIAGKGDLLPVSAMPVDGTFPTDTARWE
                     KRSIAQEIPIWDPEICIDCGKCALVCPHAAIRMKVYEPITADAAPETFQHKEWRDRDN
                     PGLWMTIQVTPDDCTGCGVCVDVCPAKSKEVVKHKAIDMMPKADHVDAERANLGFFLD
                     IPPADRREVKVASIKGSQMLEPLFEFSGACAGCGETPYLKLLSQMFGDRILIANATGC
                     SSIYGGNLPTTPWSRDANGRGPAWSNSLFEDNAEFGLGMRLALDHQVAYARALLGDLG
                     GFDEELARAILDAPQDDEAHLADQRERVAQLKRRLEAREDDDARNLLAIADALVRKGV
                     WIVGGDGWAYDIGFGGLDHVLASGRDVNVLVLDTQVYSNTGGQASKATPRGAVAKFAA
                     GGKPIGRKDLGLFATGYGNVYVAQVALGGDNAQTVKAFAEAESWPGPALIIAYSTCIA
                     HGIDMSTSMSHQAEAVKSGFWPLWRFDPRLAVPGQRPMQLDSRAPSLPLKDFQMKEAR
                     FAMLARSDPEGYAELQVLAQEDAEERRRLYEQLAGVERHAPGRAEDGGEEQAPAETEG
                     EER"
     gene            complement(11800..12690)
                     /locus_tag="VF195_00320"
     CDS             complement(11800..12690)
                     /locus_tag="VF195_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6579311.1"
                     /translation="MKILFATDGSAPAREGEGLITSLFDRSRAEIRVIAVTPEPTYDL
                     LAPYGIPEPPPPDVPVLDADRVAETAAERLAESGFTASSSTARGDPALEILKVIESGP
                     YDLVVLGASHTTWMGNLLLGRVSMHVLHHAPCSVAVTHRAPTGTGRVLLGADGSEGAR
                     SSLGSAAEVLDRDRCTIEVATVVSHPWMFAATYPAGTFLGHAPDPQGLERERIEQGRV
                     IARRASAEASAAGFKVMEEAVLVGRPGHQLLEEAENFGADLVVVGSRGLGAMSRTVLG
                     SVSDQVARHAPATFVGRLAG"
     gene            complement(12987..>13232)
                     /locus_tag="VF195_00325"
     CDS             complement(12987..>13232)
                     /locus_tag="VF195_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579312.1"
                     /translation="SAFVGRRLVRDIVVAAVGFAYAVWTMIGSGMEIIGQGFVLWMIG
                     VPVYVWMRWARRHEHELIVPFEHPELVPTTPKETVSV"
BASE COUNT         2161 a         4508 c         4428 g         2135 t
ORIGIN      
        1 cggctcaggc cctgacttcc ccgctctcgg gatctccggc cggtttcggg atccccgaag
       61 gcgagagatg ccccgtcgat cggtcgcttc ggctcgcacg ccgcgtcctc tcgggagaca
      121 catgggatcg cccggggctg acgggaatcg cgacagcgaa gcggagaggg tcagcaccga
      181 gcgacaccgc atccgccatg cgctggatcg catcgggtcc ggcgtggagg acctgcggcg
      241 ccagcatctc ccgtcgcttg acctctcgcg gatccatgaa gccgagagcg atcaccaccc
      301 ctcgttgagc cccgctgcgg ctgaagcgga ggtaggcgtg gttcaaagcc tcgtgcccga
      361 tgaccacgtc gccggaagtc cgcatgatca ccagagccga ctcacctgcc cgtaccaggt
      421 caccatcgat ctcgcagtgc acacccgcgg cctccaggat ggatcgaaca agatcgatcg
      481 ggtcatccgg gtcacggatc ctggcgcgga cggcctcggg gaggtcgaac tggtcgatga
      541 ggtctcggtg aggtggcgtg agctcgtcgg gggtgtgtcg gtcgcggatc ggtctcggag
      601 ccggttcgga tccatagagg ccgcccaaca cgttggggac gtgcgttcgc cggacgtgcg
      661 gctcgtgcag ctccggagag atcgtgagct cgcgcggagg cggtgtaccc ctgctcaccc
      721 gacccaggat cgtgccgact cgcgtccgaa cctccacttc acgggcatcg ggagaggctc
      781 tgaagatccc gagtgccgtc tcttggacca agcgtctggc cgcggaggaa gctggatcgg
      841 cttcgtcgac ttcgagggtc acggaggtca cgcgcgatga acccacccca tgagtcgagc
      901 cggtccgggg cgcggccgac cgcctggaag atctgaacgc gatcagcgcc acgagcgaag
      961 cggtcagaag gacgatcccg agcaggaccc agaagatgat caccacgcgc ccccttggct
     1021 tcttcgactc gacgctaggt gcagaggcct ccgctggcag gggccgttgg ccggtgtacc
     1081 gatgggccga acgtcacatg ttgatcacag gcaacagaga cgggccgatc ggccgtcccg
     1141 cgcaggaccg aacggcctct gggtagggct cggactcggc gtacggtcaa gatgaacacg
     1201 gaggacgagg aggcgtgaga tgaagcgtgt acgggatgtg atgacaacgg aggttgtcgt
     1261 ggtcggggat cggaccccgt tcaaggagat cgtggggctc atgcggcaac acgacgtgag
     1321 cgccctcccc gtggtcgatg cgaccgatga gctcgtgggg atcgtctcgg aagcggacct
     1381 gctcatgaag gaggagcttg tggccgaggg ggggtctcat cgccggttcc agagcagaca
     1441 gcgtcggatc gatcgagcca aggccagcgg cgtggtcgcg caggagctca tgtcatcgcc
     1501 ggtcgagacg atcgacccag aggcgccgct cgcgcgagcc gcccgactcc tccacgatcg
     1561 gcgggtgaag cgccttccgg tcgttgacgc caacggcaag gtggtgggca tcgtcagtcg
     1621 ggccgatctc ctgaaggtgt tcctccgccc ggatcgcgag atccgcgacg agatcgagga
     1681 cacgctcgcg aaggtggcgt tcgagcccgg tccggtccgc gtcacggtgg tggatggcgc
     1741 cgcgacgctc caggggcagg tcgagctgag gagccagatc cctgtgatcg tgggcatcgc
     1801 ccgagcggtc gacggtgtgg tggacgtcgc gccgcggctc agccacgagg tggacgatgt
     1861 cacgccgagc ttagaactcc tgaccccgtg gggcccgtac accccgaccg gacctcccca
     1921 ccgcgagtga tcgggccgac acgtcgggac ggatgaacgt cggccacggc tgcgccctcg
     1981 aggccgacgc cggtcaactg cgatcgcccc catcgacgtc atcctgagcc tcggggtcgc
     2041 gagggcgccg ggcatgatga gtaggactcg tgtggagagc cggcgacgag gcgtgagttc
     2101 tcagaccggt tcgtagggga ggtaccgtgg ttcccacatc atctcgtcga ccgccggtcc
     2161 gagctcgtcc tcgcggaact gccggccgac ccccgcgtcc cgtgccatcc gcgcgaccgc
     2221 caccgcgatc gcccgagagg caacccgcag atccgacggg ctggggtaga gcgacccttc
     2281 cgcgaggcgg gcctcggaga cgactctcgc cagcgcctcg gctgcgacga ggaacatctc
     2341 atcggtgact tcacgcgcct ccgcgacgag ggccccgagc ccgacccccg ggaagcagaa
     2401 cgcgttgttc gcctgaccga tcacgtgcgt ccgcccgccg cgatcgacgg gtgggaacgg
     2461 gctgcccgtc gcgacgatcg ctcgacctcg ggtccattcc aggatgtccc gtggctgcgc
     2521 ctccgtcctc gatgtcggat tcgacaacgg aaggatcacc gggagccgag catgggccgc
     2581 catctcggtt acggtcacct cgctgaagct tccgggggtt ccggatgttc cgatcaggaa
     2641 ggtcgggtgg acgtgcttca cgacctcttc gagcccatac gtctcgccgg tgacgaacct
     2701 gaatgctgcc atctcgtccc caccgagcgc gaacgcttcc ttgtcggcat cgagcggttc
     2761 tcgccccgcg tacgtcaacc ctcgcgagtc gagctggacg atcgcacgcc ggacgtcgcc
     2821 ctcgagcacg ccctcgcgca gcatctgcgc ccgcagcaga cgcgcgatcc cgatgccagc
     2881 ggcgcccgcc cccaggaaga cgaagcgctg ggcaccgagc ggctcatgga ggatccgcaa
     2941 ggctgaacgg atcccggcca tgacgaccgc ggcggtgccc tggatgtcgt cgttgaagct
     3001 cgtgatccga tgccggtagc ggtcgagcag gcggagcgca ttgtgctgct tgaagtcctc
     3061 ccactggagc acggagtgcg ggaatacctc gaggacccct tcgacgaagg cctcgatgaa
     3121 gccgtcgtac gccggtcccc gcaggcgtgg ctctcggtgt ccgaagtagc cgggatcgtt
     3181 cagcagcgct tcgttgtcgg tcccgacgtc cagcgagacc ggcagggtga gggcagggtg
     3241 gatcccggct ccggctgtgt acagagcgag cttgccgacc gggatcccca tgccccccgc
     3301 accctggtcg ccgaggccga ggacccgctc gttgtccgtc gcgacgatga gacgaacgtc
     3361 ctcgtggcgg gcgttcgaga ggagctcggg gatccgatcg acgtcctcgg gtgtgagcca
     3421 cagcccgcgc ggccttcgca tgatgtggct gtactcctgg caggctcggc cgacgaccgg
     3481 cgtgtagacg atcggcatca gctcctcgag gttgtcgagc aagagccggg agaacaaggt
     3541 ctcgttccgg tcctggagag ccgcgagccc gatgaaccgc tcgagttcgt cgcccttgcg
     3601 gcggttcttg ccgatctcga gggccacctg ctcctcgagc gtccggacgc gtggcgggag
     3661 cagcccacgg aggccgaaga cttcccgctc atgctcgctg aacgcggtgt ccttgttgag
     3721 gagtgcctgc tcgagcaact cgtgcccccg ggagggaacg gggactggtg cgctgcccgt
     3781 ccgtagtgcc tcgacccagg cgtgctcggc ggcccgggct cgttccgcgg ctgggctcat
     3841 ctcgccgcct ccttcgcggg gagcctcgag agctcgtcga ggacctgtcc gatcaccgcc
     3901 ttcgcgtcgc cgaagagcat ctgggtcttg tcgagataga acaggtcgtt gtccacgccg
     3961 gcgaaaccgg agcgcatcga gcgcttgacg acgatgatcg aggatgcggc gtcgacgtcg
     4021 aggatcggca tcccgtagat cgggctcgtc gtatcgctcc gcgcggcagg gttgacgaca
     4081 tcgttcgccc cgacgacgag cacgacgtcg gtctgcggga actcgccgtt gatctgctcg
     4141 agatcgaaca actgatcgta tggcacgtcc gcctccgcga ggaggacgtt catgtgaccg
     4201 ggcatccgac cggcgaccgg gtggatagcg aacttcacgt cgacaccgcg atgctgcagc
     4261 gcgtcggtga gctcgcggac cttgtgctgt gcctgggccg ccgccatccc atagccgggg
     4321 acgaccacga ccgatcgtgc cgcgtcgatg aggaacgctg cctcctcggc ttcgatgctc
     4381 cggaacggcc gctgttcgac atcgccggtc gcggtctcgg caccgaatcc cccgaacagc
     4441 acgttcgcga acgaacggtt catcgccttg cacatggtga tcgacaggat caacccactc
     4501 gagccgtcca gcgcgccggc gacgatcagc agttcgttgt cgagcacgaa gcccatcatc
     4561 gaggccgcga ggcccgcgaa gctgttcaag agggagatca cggtcggcat gtccgcaccg
     4621 ccgatccgtg tcaccagcaa ggtgccgaac acgagtgaga ccacgatgac gatcgggaag
     4681 agcaccgctc tgccggggtc gatcaccagg atcccgacca ggacgagagc cacgccgagg
     4741 actgacacgt tgacgaggtt ctgacctcgg tacacccacg ggtgatcggg aagggttccc
     4801 tggagcttgc cggccgcgat caacgaaccg gtcaccgtca agaagcccag gacgacctcg
     4861 agcccgatgg cgaccatggt cgtgccgtcg atctccggcc gcctgaggta gtactcggcg
     4921 gtcccgacga ggccggccgc cagcgcgccg aaggcatggg acaacgccgt cctctggggg
     4981 accgcggtca tcgggaccag caagccgagg gggacgccga gcgccactcc cgccacgatc
     5041 gccacaccga gccatcggta ctcgacgatc cggagatcga cgagcgtcgc aacgacggcg
     5101 acgagcatgc cgatctcgcc ggttcgcacg ccgcgccgcg cggtcgtggg atgcgagagc
     5161 cacttcaacg cgaggacgaa gcaggccacc gcgaacaggt aggcgagtgg gatgagggtg
     5221 tcgttcatga cgtcggtcag gacgacgtcc cctccttcgg cacttcggga gcctcacgcc
     5281 tcctgaacat cttcaacatg cgatccgtga tcaggaaccc ggagacgatg ttcgtcgtgg
     5341 cggcgaagac cgcgaccgac gcgagcacgg tcgcgaactc tgactctcgt gcccccgcga
     5401 gtatcagcga accgacgatc gagatcgcgg acagcgcgtt ggtgagcgac atcagggggg
     5461 tgtgcagcag gccggggacc ctcgagatga ccgtgtaccc gaggaacgag gcgatcacga
     5521 agatgaagag gccgacctca agcgcttggg acactgccca cctcgagaga tccgactacg
     5581 cgagggttga cgatctcgcc ttcccgggtc accagcgtgc ccgcgatgac ctcgtcctcg
     5641 agatcgatcg ccagctcccc ctggcgcacg atgtgcagca ggaaggcgac cacgttcttc
     5701 gcgtacatga cgctggcctc gtgcggcacc gtggacgcga ggttcgtcgg acccaagatg
     5761 gtcaccgagt tctctgtgac gacctcgttg tccgggtccg tgagctcgca gttcccaccg
     5821 cgctccgcgg cgcagtccac gatcacggaa ccggcgggca tcccgaggac cgcctgctcc
     5881 gtgatcagga cgggcgctct cttcccgggg atcaacgcgg tggtgatcac cacatcgacg
     5941 ccggcgacca cctgcgcgat cagctcctgc tgccgctggt agaaggcggc gccgaactcc
     6001 ttcgcgtagc ccgcaggatc ttcggcgtca cccggttgta gggggaggag cacgcaccgc
     6061 gcgccgaggc tctcgatgtc ctcttgggcg ctcgggcgca catcgtagcc ctggaccacg
     6121 gcccccatcc ggcgggccgt cgcgatcgcc gtcaggcccg cgacgcccgc cccgatgacc
     6181 aacacctgcg ccggcgggac ggtccccgcc gctgtggtga gcatggggaa catcttgggc
     6241 aagcggttcg ccgccaggag gaccgccttg tagccgatca cggttgcctg cgacgacagc
     6301 acatccatgc tctgcgcgcg tgtgatccgc ggcatgaggt ccagtgcgaa gctgatgatc
     6361 ctgcggtccg cggcttgccg gacgccttcg ggggagccga gcgggtccga tatcccgagg
     6421 accacgtgac cggggcgaag tcgatcgacg tcgtcgcgct cgttcttcgg gttcgtcccg
     6481 catgctcgaa cctgcgcgat gatggacgca ccgaacacct ccgcgcgcgc cccgatccgc
     6541 gcaccatgtg ccgcgtagga ctcatcgggg aagcccgcgc gttcgcccgc gtcgtgctcc
     6601 acgacgacat cgatgccggc gtcggacagt cggcgcacca cggagggcac cagcgccacg
     6661 cgagcttccc ccgggaacgt ctccgtgggc actcccacga cgagcctctc catggtcagg
     6721 tctgcacgct cccggggatc tctctccagt cgtacgggct cgtgaagttg acgagcatct
     6781 gcatgtagtt cgagcgcccg aacgccgagg ggtcggcgac gttcgcttga ctcacgcttc
     6841 ccttgagctg ttccacactc tcgtactcgt gctcgtccag ccacgtccgc atgccctcga
     6901 gcaccactcc caggtactcg ggcccgtgcc ggaagagggc gctcgccatc atcgtcacgt
     6961 cagccccggc gagcaggagc ttcacagcgt cgtcggccgt ccacacgcca ctcgtcgcgg
     7021 cgagagaaca tgcgaccttc ccatggagca tcgcgatcca ccgcaggggg agtcgcagct
     7081 catcgctcgt cgagaggtgg agggtcgggt cgacccgcag cgtgtcgagg tcgatgtccg
     7141 gctggaggaa ccggttgaag agcacgagcc cgttcgcccc ggccgcttcg agctgcagcg
     7201 ccatgttcgc catggatgag aagtagggtc cgaccttcac ggcgagcggg atcgagacgc
     7261 ttccacggac ggccgagacg aggtcgaggt atcgccgctc gacctggctg ccgctctcgt
     7321 cgggatcggc cgcgatgaag tacacgttca actcgagcgc atcggcaccc gcgtcttgga
     7381 tcagcctcgc gtagcggatc catccaccct cgctcgcccc gttcagggag ccgatcacgg
     7441 ggatctcgag ctcccgcttg cacgcggcga ggtgtcgcag gtactcgtcg gggccggtgt
     7501 tgtactcctc catctccggg atgtacgaaa gggcctccgg gaacgactcc gcaccgttct
     7561 cgaggacgcg gtgcgtctgg agttcctcgt gctcgatctg ctcctcgaac aatgactgca
     7621 tcacgatcgc cgacgcaccc gcttcctgga ggcgcttgag gttgtcgatc cgcgacgaca
     7681 gtgtggaagc cgacggcacg agcgggttcg cgagctcgag ccccaggtac gtggtccgaa
     7741 ggtcgacgct catctctctt ccccttccgt ctctgcgggc gcttgttcct ctccgccgtc
     7801 ctccgcgcgg cccggggcgt ggcgctcgac cccggcgagt tgctcataca gcctccgacg
     7861 ctcctcggcg tcttcctgcg cgagtacctg gagctctgcg tagccttccg gatcggagcg
     7921 ggccagcatc gcgaaccgag cctccttcat ctggaagtcc ttcagcggca gcgacggagc
     7981 cctgctgtcg agctgcatcg gacgttgccc cgggacggcg agacgcgggt cgaaccgcca
     8041 gagcggccag aagccgctct tgaccgcctc ggcctggtgc gacatcgagg tcgacatgtc
     8101 gatcccgtgg gcgatgcacg tcgagtacgc gatgatcagg gccggccccg gccacgactc
     8161 agcctcggcg aacgccttca ccgtctgcgc gttgtccccg ccgagcgcga cctgcgccac
     8221 gtagacgttc ccgtaccccg tcgcgaacag ccccaggtcc ttgcgtccga tcggcttgcc
     8281 tcccgccgcg aacttcgcga cggctcctcg tggcgtcgcc ttggacgcct gcccgccggt
     8341 gttcgagtag acctgcgtgt cgagcacgag gacgttgacg tctctcccgc tcgccaacac
     8401 gtggtcgagc ccgccgaaac cgatgtcgta ggcccagccg tcgccgccga cgatccatac
     8461 gcccttgcgg acgagcgcat cggcgatcgc gagcaggttc cgagcgtcgt cgtcctcgcg
     8521 agcctcgagc cgtcgcttca gctgtgcgac gcgctcgcgc tggtcggcga ggtgagcctc
     8581 gtcgtcctgg ggtgcatcga ggatcgcgcg ggccagctcc tcgtcgaacc cgccgagatc
     8641 gccgagcagg gcgcgcgcat acgccacctg gtggtccaga gcgagccgca tcccgaggcc
     8701 gaactccgcg ttgtcctcga acagggagtt cgaccacgcg ggtccccggc cgttcgcatc
     8761 ccgtgaccag ggcgtcgtcg gcaggttgcc gccgtagatc gaggagcagc cggtggcatt
     8821 cgcgatcagg atccggtccc cgaacatctg tgacaggagc ttcaggtagg gggtctcgcc
     8881 gcaccccgcg caggccccgc tgaactcgaa gagcggctcg agcatctgcg agcccttgat
     8941 cgaggcgacc ttcacctcgc gccggtcggc cggagggatg tccaggaaga atccgaggtt
     9001 ggcccgctcc gcatccacgt gatcggcctt cggcatcatg tcgatcgcct tgtgcttcac
     9061 gacctccttc gacttggccg ggcagacgtc gacgcagacg ccacatccgg tgcagtcatc
     9121 gggagtgacc tggatggtca tccacaagcc cgggttgtct ctgtcgcgcc actccttgtg
     9181 ttggaacgtc tcgggcgccg catcggcggt gatcggctcg tacaccttca tccggatcgc
     9241 cgcgtgcggg cacaccagcg cgcatttccc gcaatcgatg cagatctccg ggtcccagat
     9301 cgggatctcc tgcgcgatcg agcgtttctc ccatctcgcg gtgtcggtcg ggaacgtccc
     9361 atcgaccggc atcgcagaga ccgggagcag gtcgcccttc cccgcgatca tcatggcggt
     9421 cacgcgctgg acgaagtcgg gagcgtcctc cgggaccggc agcgatcggt gcacgtcacc
     9481 gccgatgacc gccggcacgg gaacctcgtg gaggccgttg agagcggcgt ccaccgcggc
     9541 gaaattctgc tcgacgacgg cacggccgcg ctttccatag ttcttctcga tcgcgacctt
     9601 gatccgagcg acggcctcag cctgatcgag gacgttcgcg agggcgaaga agcacgtctg
     9661 caggatcgtg ttcacgcgca ggccgagtcc cgcggccgtc gcgaccttct gggcatcgac
     9721 gacgtagagg cggagctcct tctcgaggat cgcctgctgg gcctcgacgg ggaggtgctc
     9781 ccagaccgaa cccgggtcga acggacagtt cagcagcaac gtggcgccgc gggctgccac
     9841 gtcgaggaca tcgaggcgct cgaggaagcc gaactgatga caggcgacga agctcgcctg
     9901 gcccgagccg atcaggtacg tcgagtggat cggccgcggt ccgaaccgca ggtgcgaggt
     9961 cgtcacggag cccgacttct tggagtcgta gacgaagtag ccctgggcga acaggtcggt
    10021 cccctcgccg atgatcttca ccgagctctt gttggcgctc accgttccgt cggcgccgag
    10081 gccgtagaac accgctcgca cgacatcgtc ggcctccgtc gagaaggatc ggtcgacctc
    10141 gagcgaggtg tgcgaaacgt cgtcgacgat gccgacggtg aagtggttct tgggcctgtc
    10201 cttcgcgagc tcgtcgaaga cagctttggc catcgccgga tcgaactcct tcgatgcgag
    10261 gccgtaccgg ccaccgacga ccgtcggcat cgtgctgatg gcaccggaga cgatccgctc
    10321 ggcgagcacc gtcacgacat cctgatagag aggctcgccg ggtgcgcccg gctctttcgt
    10381 ccgatcgagc acggcgatcg cgcgaacggt ggtcggcaac gctccgagga atgcctctgc
    10441 cgagaacggc cggaagagcc ggacgttgag gatgccgacc ttctctccgc gtgcgacgag
    10501 ttcatcgacg gtctcgcgcg cggcaccctc acccgagccc atgagcacca cgacgcgttc
    10561 cgcgtcggcg actccgtgat agtcgaacag atggtaggcg cgaccggtga gctccgcgaa
    10621 tcggtccatc gtctgctgca ccacctgcgg gatgatcgcg tagtaggggt tcgaggcctc
    10681 ccgggcctgg aagaacacgt ctgggttctg ggcggtgcca cgcaggaccg ggtggtcggg
    10741 ggagagggcg cgctcgcgat gcgcccggac catgtggtca tcaacgagct cgcgaagcgc
    10801 ctcgtcgccg agctcggcga tcacgttcac ctcgtgcgag gtccggaagc cgtcgaagaa
    10861 gtggaggaac ggcacacgac actcgagtgt cgccgtgtgg gcgatcgcgg cgaggtcctg
    10921 agcggcttgg acatccgacg aggcgaggat cgcccaaccc gcctgtcgca cagccatcac
    10981 atcgctctgg tcgccgaaga tcgaaagcgc atgggtcgcc aacgtccggg cggcgacgtg
    11041 gatcaccgtt ggggtgagct ctccggcgat cttgtacatg ttggggatca tcaacaggag
    11101 gccctgcgat gccgtgaacg tggtgcacaa cgcgcctccc tgcagcgcgc cgtgcagtgc
    11161 acccgcggcg ccgccctcgc tctgcatctc ggtcacctgc gggatggcgc cccagaggtt
    11221 cgtgcggccc gccgccgacc aggcgtcggc gagctcgccc atcgacgtgg acggcgtgat
    11281 cgggtagatc gcgatcactt ccgacagtcg gtgagcgatc ctcgccgctg cctcgttccc
    11341 gtcgaccgcg atcatgcttc ggccttccgc catccggacc tcccagcagt ctcctcgcga
    11401 ccgggacgcg ccgccccggt cacgcggccc gtctcgcggg ggtcgttcga cccctcgcgc
    11461 gcgatcagaa gttcccccgc cgacggaggg ccgtgtcgcg acaccgagat atcgactcga
    11521 gaccgatgag cgatgctctc agcatgtcgg ccactcgacc tagcctcagc aggcggggtc
    11581 atggtaagcc ttcgcactca tcgtggagcg cgcgtgggtt ccctctggcc accgcagcgt
    11641 cggcgtccgc cccacacggc aaccgggact gttgcccgca gccgcggtgg tcgatcagga
    11701 cggtccgaac acgccatccg accacagggt ggcgacacca cggtcgcgcg atccgaccgt
    11761 cacccgggca tcgctgaggg atcgccgtgc ggggaacgct cagccagcga ggcgacccac
    11821 gaacgtcgcc ggagcatgcc gggcgacctg gtcgctcacg gatcccagga cggtgcggct
    11881 catcgctccg agccctctcg agcccaccac caccaggtcc gctccgaagt tctcggcttc
    11941 ctccaggagc tgatgccccg gtctgccgac caggaccgcc tcctccatca ccttgaaacc
    12001 ggcggcgctc gcctcggccg aggcgcgccg ggcgatgacg cgaccctgct cgatccgctc
    12061 acgttcgagg ccctgggggt cgggggcgtg tcccaggaac gtgccggccg ggtaggtggc
    12121 ggcgaacatc caggggtggc tcacgacggt cgccacctcg atcgtgcatc gatcgcggtc
    12181 caggacctcc gctgcggacc cgaggctcga gcgggctccc tcggagccat cggcgcccag
    12241 gaggacccgt cctgtgcccg tcggcgcacg gtgtgtcacg gcgaccgagc atggagcgtg
    12301 atgcagcacg tgcatgctga cccggccgag caggagatta cccatccagg tggtgtggga
    12361 ggccccgagg acgacgaggt cgtagggacc gctctcgatg accttcagga tctccagcgc
    12421 aggatcgcca cgcgcggtgc tcgaggaagc cgtgaacccg ctctcggcca ggcgctccgc
    12481 ggctgtctcg gccacccgat cggcgtccag gaccggcacg tccggaggag gcggttccgg
    12541 gatcccgtat ggagcgagga ggtcgtacgt gggctccggt gtcacggcga tgacacggat
    12601 ctccgctctg gatcggtcga acagcgaggt gatcaggccc tcgccttcgc gggccggagc
    12661 ggacccgtcg gtcgcgaaga ggatcttcat gtcgactcct ttcctcccca gcatccgggt
    12721 cgatctggtc tctcgggagg tccacccgac cctgattcca ggggcagtag ggactccgtt
    12781 cgccggtcct gcctgggtcg tgatcgatgc ttgagtgcat cgatcacccc cgcgtgaggg
    12841 ttccctcccg cccggcgatc cgacgatcac ctggagccta gcggggcacg gccatggctc
    12901 tgaggccgac cagccccgtg accccgaaac cttcgatcgg ggatggaaag ggcctccgtg
    12961 cggaggccct ctcctttcgg ccggtctcag acgctgaccg tttccttcgg tgtggtcggg
    13021 acgagctcgg ggtgctcgaa cggcacgatc agctcgtgct cgtgacgacg cgcccaccgc
    13081 atccacacgt acaccggcac gccgatcatc cagagcacga agccctgtcc gatgatctcc
    13141 atgcccgaac cgatcatcgt ccacaccgcg taggcgaatc cgacggcggc cacgacgatg
    13201 tcgcggacga gccgccggcc gacgaacgcg ga
//
LOCUS       DASUYD010000007         3980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_886266, whole genome shotgun sequence.
ACCESSION   DASUYD010000007 DASUYD010000000
VERSION     DASUYD010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886266"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..247)
                     /locus_tag="VF195_00330"
     CDS             complement(<1..247)
                     /locus_tag="VF195_00330"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX6579313.1"
                     /translation="MESRVRSVPEVAEEATLAEQAPSFEEFYGANFRHLFTALCLVTG
                     NRHEAEEITQDSFLKIYERWDRVGALDDPTAYLFRVSM"
     gene            complement(444..731)
                     /locus_tag="VF195_00335"
     CDS             complement(444..731)
                     /locus_tag="VF195_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579314.1"
                     /translation="MSLRASGDDSGARDRQTPEETITAERGDLGEVQVVAKRADELVS
                     GDPDDHRPNDGDDDDIPPAILLHILMLPDFDQATGSASSGATRLAAPSQSS"
     gene            1077..1928
                     /locus_tag="VF195_00340"
     CDS             1077..1928
                     /locus_tag="VF195_00340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX6579315.1"
                     /translation="MRRTLIVGAVTMSIGLASVPMATAITFGEPDEDRHPNAGAMLAD
                     FGGDVVPFCSGSLIASDVFLTAAHCIVGVEQIAEEEGVEPEFLVSFDEELGEFPDGVF
                     DIHGFDLASGVELISGTPFFDERFGTAGASQPFDIGVILLDTEPGIQPVDLPTEGFLS
                     GLKAQHDLRSETFTAVGYGAVRDDFTGGFENILNEEVVRLVATQSALNLQKQWLLLSM
                     NPNTGSGGTCYGDSGGPHFAHGINPNLQVSVTITGDAVCKATDLTYRLDTPEARKFLS
                     EFVDLPT"
     gene            complement(2026..3312)
                     /locus_tag="VF195_00345"
     CDS             complement(2026..3312)
                     /locus_tag="VF195_00345"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6579316.1"
                     /translation="MNAAAGEEFVLLAIPDMNGSLRGKALRPDAFESALEHGTVMTDL
                     IIGLDPVDTPITDYERFGIRVGAPDLIVRPESDTLRDLPWRPGWRVCLATPNWPDGSP
                     AFASREVFRRVLDDVDTLGFEVMAAIEYEIRIWDEDGAPASSGISYSLNEIGRYGRFL
                     DELAPALEGLGIELSAVHTEAGPGLLELNVEARRGLAAADDAALLKFGVKQVAASMGL
                     RASFLAKTNPGEEGSSGHVHLSLWRRTTNAFAGADRSAELVPPFSSAVAGVVEHLPGA
                     SLLLNPTINSYKRLVPGWFAPINATWGYENRSCAVRAIRGARPELWRFECRRPGADAN
                     PYLVLAAIGASVADGIRHEATPPNPIQGDAYALDLPELPGSLDAALGSFEDDAALREA
                     LGKDFSDYYATSRAWELKAWRETVTEWERERYDRSV"
     gene            complement(3309..>3980)
                     /locus_tag="VF195_00350"
     CDS             complement(3309..>3980)
                     /locus_tag="VF195_00350"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6579317.1"
                     /translation="RRQEHGFLVCFDEVVTGIGRVGSWLAAHQLPIEPDIVAVGKGLG
                     AGYAPLGAVLVREEVYDAIDRGSREFDLGHTWDGAPLPSAVGLAVLDLIEERALVDRV
                     SDRAPALRDELASVLEGSGIVKEVRGRGFLLGVELVDPRDGTSFLPVDLDAAAIVDDT
                     AFEHDLLVTSTHPQADGFAGDQVLLAPAYTSTDAELAEMVERFAATMNDVEHRVGARL
                     AGSPS"
BASE COUNT          659 a         1351 c         1310 g          660 t
ORIGIN      
        1 tcatcgagac cctgaacagg taggcggtcg gatcgtcgag tgcaccgacc cgatcccacc
       61 gctcgtagat cttcaggaag gagtcctggg tgatctcctc ggcttcgtgg cggttccccg
      121 tcacgagaca aagggcggtg aagaggtgtc tgaagttggc tccatagaac tcctcgaagc
      181 tcggagcctg ctcggccaac gtggcctcct cggccacctc aggcacggat cgcacgcgcg
      241 actccatcgt gctccttgaa cacatgggag gccagcgacg ctgcaaacat gggaaacatt
      301 catcaaactg tgctcgagcg tgcagcggat tgccagagcc agttttgcgg attcggttga
      361 ctgccttgtt gagagtcagc gatcggcatc ccgcaacttg ccgatgagca ccgcccgcac
      421 cgtccggtcc tcctcgcagt cgatcaggag ctctgcgaag gtgcggctag ccgggtagcc
      481 ccagaactcg ccgatccggt ggcctggtcg aagtcgggga gcatgaggat gtgaaggagg
      541 atggcgggtg ggatgtcatc gtcatcaccg tcgttgggac ggtggtcgtc ggggtcacct
      601 gaaacgagtt cgtcggcacg tttggcgact acctgaacct cgccaaggtc ccccctctcg
      661 gcggtgatcg tctcctcggg ggtctgtcgg tcgcgcgctc ccgagtcgtc tccactggca
      721 cggaggctca catcatcaat ctcatccgct cctactccct cccctggaac gatgtctcaa
      781 gtttcgaacg agtcgacgat gggtcggtga aagtgcgatc cgcatccggc cgagccattt
      841 tcggcttgga gccttgagcc tgtggcgctt tccgcgtcaa cgacacggat ggcgtgcgac
      901 ggcgttgacc gagctggagg caatcaatcc tcgccgcgcg ccaacgccag tactgaatca
      961 cgcggcttcg ggctgctctg tgactggcga aggagtgcat ccgctacctc atatgggggt
     1021 tgaccgaaaa ggctggacgc cactacggtg ccgggccgtc gatagaggga ggagccatgc
     1081 ggaggacgct catcgtcggc gcggtcacga tgtccatcgg cctcgcgagc gtgcccatgg
     1141 cgacggccat aaccttcggc gagccggacg aagaccggca tccgaacgcg ggcgcgatgc
     1201 tcgccgattt cggtggcgac gtggttcctt tctgttcggg atcgctaatc gcatcggacg
     1261 tcttcctgac cgccgcccac tgcatcgtgg gcgtcgagca gatcgccgaa gaggaagggg
     1321 ttgagccaga gttcctcgtc agcttcgacg aagagcttgg cgagttcccc gacggcgtgt
     1381 tcgatatcca cggattcgac ctcgcatctg gggtcgagct gatctccggt actccgttct
     1441 tcgatgagcg tttcgggacg gcgggtgcct cacaaccgtt cgacatcgga gtgatcctgc
     1501 tcgacacgga gccagggatc cagccggtag acctccccac cgagggcttc ctttcagggc
     1561 tcaaggcaca gcacgacctc cggagcgaga cgttcacggc cgtcggatac ggagccgttc
     1621 gagatgattt caccggaggc ttcgagaaca tcctgaacga ggaggtcgtc cgactcgtcg
     1681 cgacgcagtc ggcgctcaac cttcagaagc aatggctcct gctgagcatg aacccgaaca
     1741 ccggcagcgg tggcacctgc tacggggatt caggcggccc ccacttcgcc cacggcatca
     1801 acccgaacct ccaggtttcc gtcacgatca ccggcgacgc agtctgtaag gcaacggatc
     1861 tcacgtaccg cttggacacg cctgaggcgc gaaaattcct ctcggagttc gtcgacctcc
     1921 cgacgtagct tgatgtcttg gatctcgctt ccgcagcacc tgggtcttcc gtcgccgcca
     1981 tcactctctt aagcccggcg gagcacgact catcgcgaat tgccgtcaga cgctacggtc
     2041 gtagcgctcg cgctcccatt cggtgaccgt ctcgcgccag gccttcagct cccaagctcg
     2101 agacgtcgcg tagtagtcgc tgaagtcctt gccgagcgcc tcgcggagcg ccgcgtcgtc
     2161 ctcgaacgat cccagggcgg catcgagcga gcccggcagc tccggaaggt cgagcgcgta
     2221 ggcgtcgcct tggatggggt tgggtggggt cgcctcgtgc cggatcccgt ccgcgaccga
     2281 ggcaccgatc gcggcgagca ccaggtacgg gttcgcgtcg gcgccgggcc ggcggcactc
     2341 gaaccgccag agctccgggc gcgcgccgcg tatcgcgcgg acggcgcacg aacggttctc
     2401 gtagccccac gtcgcgttga tcggcgcgaa ccagccgggg accaggcgct tgtacgagtt
     2461 gatcgtgggg ttgaggagga gcgacgcgcc cggcaggtgc tcgacaacgc ccgcgaccgc
     2521 cgacgagaac ggcggcacaa gctcggcaga ccgatccgcg ccggcgaacg cgttcgtcgt
     2581 ccttcgccag agggacaggt gcacgtggcc gctcgagccc tcttcgcccg ggttcgtctt
     2641 cgcgaggaag ctcgcacgca gacccatgga cgccgcgacc tgcttcacgc cgaacttcag
     2701 cagcgcggcg tcgtcggccg ccgcgagccc tcggcgggcc tcgacgttca gctcgagcag
     2761 acccggtccg gcctccgtgt gcaccgcgga cagctcgatc ccgaggccct cgagcgccgg
     2821 ggccagctcg tcgaggaacc gaccgtaccg gccgatctcg ttcaggctgt agctgatccc
     2881 gctcgacgcc ggcgcaccgt cctcgtccca gatccggatc tcgtactcga tcgccgccat
     2941 cacctcgaaa cccaaggtgt cgacgtcgtc gagcacgcgg cggaacacct cccgcgaggc
     3001 gaacgcgggc gagccatccg gccagttcgg cgtcgcgagg cacacgcgcc agcccggtcg
     3061 ccacggaagg tcgcggagcg tgtcggattc ggggcgcacg atcagatcgg gggcccccac
     3121 gcggatgccg aagcgctcgt agtcggtgat cggggtgtcg accgggtcga gcccgatgat
     3181 caggtcggtc atcaccgtgc cgtgctcgag cgccgactcg aacgcatccg ggcgcagggc
     3241 cttgccgcgc agcgacccgt tcatgtcggg gatcgcgagg aggacgaact cctcgcccgc
     3301 ggcggcgttc acgacggcga cccagcgagc cgggccccga cccggtgctc gacgtcgttc
     3361 atcgtcgcgg cgaaccgttc gaccatctcg gcgagctccg cgtcggtgct cgtgtaggcg
     3421 ggcgccagga gcacctggtc cccggcgaac ccgtccgcct gtggatgcgt ggacgtcacg
     3481 agcaggtcgt gctcgaacgc ggtgtcgtcg acgatcgccg cggcgtcgag gtcgaccggc
     3541 aggaaggacg tgccgtcgcg cgggtcgacg agctcgaccc cgaggaggaa gccgcgcccg
     3601 cggacctcct tcacgatccc cgaaccctcg agcaccgagg cgagctcgtc tcgcagggcc
     3661 ggtgctcggt cgctgacccg gtcgacgagc gctcgctcct cgatcagatc gagcaccgcg
     3721 agcccgacgg ccgagggcag cggcgcgccg tcccacgtgt gcccgaggtc gaactcgcgc
     3781 gacccgcggt cgatcgcgtc gtagacctcc tcccggacca ggacggcacc gagcggcgcg
     3841 tagcccgccc ccagcccctt gccgacggcc acgatgtcgg gctcgatcgg gagttggtgc
     3901 gcggccagcc agctcccgac gcggccgatg ccggtcacca cttcgtcgaa acagaccagg
     3961 aacccatgct cctgacggcg
//
LOCUS       DASUYD010000008         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1107075, whole genome shotgun sequence.
ACCESSION   DASUYD010000008 DASUYD010000000
VERSION     DASUYD010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107075"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF195_00355"
     CDS             complement(<1..341)
                     /locus_tag="VF195_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579318.1"
                     /translation="MGSVRVSRRLGAIVAFGAAAAAIVVAVVALLERPLLLLATLACL
                     GIAIGAATFALTRMGWRRSVGFALAIVALLTPVVLAVVVGGALPAVLFVLLEVVAAGA
                     ARYALGRDVASL"
     gene            374..1030
                     /locus_tag="VF195_00360"
     CDS             374..1030
                     /locus_tag="VF195_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6579319.1"
                     /translation="MSSDPRKLDRSMADAVARRVDSRRGEASPWSTALRELGAVDRAT
                     YRAIAETPTPSLDDPIRRLSNAANNSRLWLGIAAALAVLGGRRGRRAALEGVVAIGVT
                     SATVNLLAKSLAARPRPRREELEPFPSRAVEMPASSSFPSGHAASAFAFSYAIGRHMP
                     ELAVPVRLLAGAVAYSRVHIGVHYPGDVTVGAMMGSGIAAMLSSLGDRLAIRRRRSNV
                     "
     gene            1069..1497
                     /locus_tag="VF195_00365"
     CDS             1069..1497
                     /locus_tag="VF195_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX6579320.1"
                     /translation="MSLQPTVRAPRNPALLEHAKERRAKFENRLADTITRFAGSMDFV
                     YLSIVWYGLWIAIPLEEYPFGLLTMIVSLEAIFLSTFILISQNRADERRQVVADHQWE
                     TVEAREAQIEQLMSIATEMLQLTRAIHERTMSQPGGEESS"
     gene            complement(2034..2300)
                     /locus_tag="VF195_00370"
     CDS             complement(2034..2300)
                     /locus_tag="VF195_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579321.1"
                     /translation="MASGTQRGRACEADGWTKEVLPTVKVALGRLQFDLASRLLQADA
                     VLLGLQEIERTTRWTVGRHTLDGGAGTDACANGEELIDSRAPDA"
BASE COUNT          425 a          878 c          848 g          426 t
ORIGIN      
        1 agcgacgcga cgtctcggcc gagcgcgtag cgtgccgcgc cggctgcgac gacctcaagc
       61 aggacgaata ggaccgccgg gagagcgcct ccgacgacca cggccagcac caccggggtc
      121 aggagcgcca cgatcgccag ggcgaaaccc acgcttcgtc gccatcccat ccgcgtcagc
      181 gcgaacgttg cagccccgat cgcgatcccg aggcatgcga gcgtggcaag aagcaggagc
      241 ggccgctcga gaagggcaac gacggcgacg acgatggcag cggctgccgc gccgaacgcg
      301 acgattgcgc cgagccgtcg agataccctg accgaaccca atcgctcctc ccggtcgact
      361 tagaatcctg cgcatgtcgt cggatccgcg caaactcgac cgcagcatgg ccgacgccgt
      421 cgcgcggcgg gtcgacagtc ggcgcggaga ggcgtcgccg tggtcgacgg cactgcgcga
      481 gctcggcgcc gtggatcggg ccacatacag agccatcgca gagacgccga cgccctccct
      541 cgacgatccg atccggcggt tgtcgaacgc ggccaacaac tcgcgactgt ggctcgggat
      601 cgccgccgca ctcgccgtgc tcggggggcg gcgcggtcgt agggccgcgc tcgagggcgt
      661 cgtcgctatc ggagtgacct ctgcgaccgt gaacctcttg gccaagtcgc tcgccgcgcg
      721 accgcgaccc cgtcgcgagg aactcgagcc gttcccttcg cgtgccgtcg agatgcccgc
      781 gtcctcctcg ttcccctctg gccacgccgc gtcggcattc gcgttctcct acgccatcgg
      841 tcgccatatg cccgagctcg cggtcccggt ccggctcctc gcgggcgcag tcgcctactc
      901 gcgggtgcat atcggcgtcc actacccggg cgacgttacc gtgggggcga tgatgggatc
      961 ggggatcgcc gcgatgcttt cctcactcgg cgatcgcctc gcgattagac gacgtcgctc
     1021 gaacgtctga aacactccgg gaatcggtgg ctgaagaggg aggatgagat gagtctgcaa
     1081 ccgacggtcc gagctccccg gaatccagcg ctgctcgagc acgcgaagga acgacgggcc
     1141 aagtttgaga atcggttggc cgacacgatt acccggttcg ccgggtcgat ggacttcgtt
     1201 tacctgagca tcgtctggta cggcctgtgg atcgcgatcc ctctcgagga gtatccgttc
     1261 ggtctcctca cgatgatcgt gtcgctcgag gcgatcttcc tgtccacctt catcctgatc
     1321 agccagaacc gggcggacga gaggcgacag gtcgtcgccg accatcaatg ggagaccgtc
     1381 gaggcgcgag aagcccagat cgagcaattg atgtcgatcg cgaccgagat gttgcagctg
     1441 acccgcgcaa tccacgagcg aaccatgtcc cagcctgggg gcgaagagtc gtcgtgaggc
     1501 ggggtaacgc taccctgccg gtgaggctcg gctaaaccga tccgtagcgg cggccattct
     1561 gagttgactc gggatctggc atcggccacg aagcggccgc gagttcgcat ccgttccggc
     1621 gggaagccag tgcccacccg cgttttcgcc atttgtgggg atactccgcc cgatcgagcg
     1681 cacgcctcat tcggtggtcc ggcggtagcc cgggggctgg agggaacggg ccatgagcag
     1741 gaaggcgaaa agggtggacg accggtctgg tttcccccgt cattctcgca gtgggggttc
     1801 ttcgcctacc agtggctcac gttcaactgg gccaagcgac ggcgggcccg ggcagcgttt
     1861 tggcccatcc ctggttgctc tgatctccct gtatgcggtc ccgcagctac gcggtcctgc
     1921 gcccaatcgg gcctagaaga gcgtcccgtc aaggtgcatt cacgcaccct cacgcacacc
     1981 acggggttga ccccagcctc gccattcgcg gacaaggttc cctacgcgta gacctacgcg
     2041 tcaggtgctc tcgagtcgat gagctcctca ccgttcgcgc aggcgtcggt tccggcaccc
     2101 ccatcaaggg tgtgccgtcc caccgtccag cgtgtcgtcc gctcgatctc ctggagaccg
     2161 agcaggaccg catcggcttg gaggagccgg ctggcaaggt cgaactggag acgaccgagg
     2221 gcgaccttga cggttggcag gacctccttc gtccagccat ccgcctcgca ggccctgcca
     2281 cgctgcgtac ctgaagccac ggccgccggt gcgccaccgc ttcaacgacg cggtgctcga
     2341 ggctgtctcc atcagggacc gaaagatcac ccggacggag ttctccgagg tcttcgcgcc
     2401 tctcttctct cgtccgagtt cgaatagcgc gctgagagtg gaggtggggg gagtcgaacc
     2461 cccgtccccg ggcgaccttt cagggcttct ccgggcgcag ccggtgggaa gatctcgctt
     2521 cgaggctccc accggcggag gacctctcgg ccagcccggg tgcgatgtcc ggcggcg
//
LOCUS       DASUYD010000009        21503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_447172, whole genome shotgun sequence.
ACCESSION   DASUYD010000009 DASUYD010000000
VERSION     DASUYD010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21503
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447172"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VF195_00375"
     CDS             complement(<1..1260)
                     /locus_tag="VF195_00375"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012947864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HEX6579322.1"
                     /translation="MDRAFDYVIVGGGSAGSVLANRLTTDPSTRVLVLEAGRPDYLWD
                     VFIHMPAALTYPIGWRWYDWKYESEPEPNMGGRRISHARGKVLGGSSSINGMIFQRGN
                     PLDYERWAADPGMETWDHAHCLPYFKRMENCLAADPADAYRGHSGPLILERGPARNPL
                     FGAFFEAVQQAGYPLTDDVNGYRQEGFAPFDRNVHRGRRLSAARAYLHPVSSRPNLEI
                     RTRVMVNRILFEGIRAVGVDYSHGPGPSRRVRAGEVILCGGAFNSPQTLQLSGVGSAG
                     ELASLGIPVVQDLPGVGEHLQDHLEVYVQHACTQPVSVAPGLKNWRKPFIGAEWLFFR
                     SGLGATNHFEAGGFVRSNEDVAYPNLMFHFLPIAIRYDGSAPAGGHGYQVHIGPMYSD
                     ARGSVKVVSTDPRVHPAIRFNYLSTDQD"
     gene            1328..2095
                     /locus_tag="VF195_00380"
     CDS             1328..2095
                     /locus_tag="VF195_00380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579323.1"
                     /translation="MDGSPLAAADPFEVSVGVVREANARGIPLKLLGGQAVRLLCPLF
                     PHRARDDQDMDFACISSKKRDVIAFFAEQGFLGDQRFNTLHGDRQMYFTTADGKTSVD
                     VVMDRLNMCHVLDFHDRIDRLPDTLDVADLLLTKLQVFELNRKDVHDILHLFSGFPVA
                     EGDEPGTIGTDRVGTLVGEDWGWWRTATMNLEKVILYSEGRPPDLVPEDSPFDPSAQA
                     RTLLGVCSEAPKTLKWKLRAKVGDRVQWYQLPEEVGH"
     gene            2166..3110
                     /locus_tag="VF195_00385"
     CDS             2166..3110
                     /locus_tag="VF195_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6579324.1"
                     /translation="MKIFFVTDLHGSEICWKKFLNAGSFYGADVVILGGDVTGKAMVP
                     IVQRPDGTWEASLQDHRDTLESEDAIVEFEKRVMNRGYYPIRVSDQEYVAMQEDEDLV
                     DKRFKEVMLDGTERWIAMAEEKLADTGIRVIACPANDDMFEIDDLLAGARVVETGDEE
                     HPIQLGSFTMVSMGWTNPTPWNTFREAEEPELATRIDRSLEAATDPETTIFNFHAPPY
                     GSKLDNAPALNPDLTYVSGGQAIRPVGSTSVHEAIKRFQPMLSLHGHIHESKGATRIG
                     KTLALNPGSSYEEGILQAAVVTIDDKKRKVKNYQLVNG"
     gene            3146..4804
                     /locus_tag="VF195_00390"
     CDS             3146..4804
                     /locus_tag="VF195_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6579325.1"
                     /translation="MAAVGEAPTLFLRKATGLVKGWSKFDAFLYSFMSVNFVTLGLFF
                     SLAVIGYVPEGQVLPALVISGLFISCIVVTYAGLISIMPRAGGDYVWQSRVFGGGLAF
                     VLSVTGWWFILWYWTPIYASILNVEILQPLAAFLGADGLLEFLGKDIGLFTICVVTAL
                     LAGYLVSLGMEGYARIQKVCFYIGLAALAVVFVVMLFGSKAGFEAAFNQEAGDLFGTT
                     GNAYRDTIAFAGGGAALPADIGLTPFFGKSLLIIPFLCFWILWPNWGSTLYGEVRGAS
                     DFKRVMSGMMWGLWVTIAIAAVFLLLSSKFFGWDFFNAGNVNFINYFYGYTDSPGPTP
                     VWTYPPLLVGMFFHNDLIGALLVLAFGAWFLGWAGTLFLSSTRVIFAAAFDRILPEKV
                     ADVSERRHVPVWALILMLVPSLIVSAFFAFTTVMKTWIIDATLVIAVTFFGTAIAAMI
                     LPWRKKRLYESSAMARYKIAGIPLITVTGALSALFLGYNLYKWLWPPYNSPDGNLYAI
                     NVPDSLWFMGSMYLLALLIYVVAKFVRRSQGIDLSAIHKEIPVE"
     gene            4883..5854
                     /locus_tag="VF195_00395"
     CDS             4883..5854
                     /locus_tag="VF195_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6579326.1"
                     /translation="MGDAPTRVFFAADIHGSQVTFRKFLAAAAFYCVDALVFGGDLMG
                     KALVPILRTNGGYRASFQGRDEEFDADGLGAFTDALEQPGFYWKVFDREQYEAVEHDP
                     MAKHGLFHELASERLAGWIERAEEALHGTGVRLYLTGGNDDEPSVLETLDRHDGDRVV
                     ASEGRLVDLDGEHTMITVGVSTYTPWDTPREASEEEIARAIEEAVATVPDVGRCVFNL
                     HCPPKDTPIDTCLKLEVRPGELPRPIREGGRFVTTGGGSTAVREAIERYQPLVGLHGH
                     IHESGGRFRIGRTQTFNPGSEYVQGVLQGVIVSLRRGRLVSYQHTQG"
     gene            complement(5868..6263)
                     /locus_tag="VF195_00400"
     CDS             complement(5868..6263)
                     /locus_tag="VF195_00400"
                     /inference="COORDINATES: protein motif:HMM:NF044909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579327.1"
                     /translation="MPPEDRGRIQTFLERYGRAIGSGDLVGAAACWVVPALVLSDEGA
                     IPVESRDEIEAFFGQAVAAYRSQGLVETRPEIERIEVLSDRLVSVDVRWPAFDATGEE
                     RVSERSRYVLRSGGDGDPQIQVALMKANT"
     gene            6320..8779
                     /locus_tag="VF195_00405"
     CDS             6320..8779
                     /locus_tag="VF195_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6579328.1"
                     /translation="MRERVQNVVIGAGIVGTSAAYHLAELGITDVLVLDQGPLFETGG
                     STSHAPGLVFQTNGSRTMCRLAQYSVELYAGLETGGLPAWYGVGGIEVATTRERMLEL
                     RRRRGYALAYGIEGTELLTPDETAARIPILDPGSILGSYLVPSDGIAKGIRVAAALGA
                     NAQAKGVAFEGGVRVTGFDIRDGRIHGVRTDRGDIECDQVLVCAGIWGPTIGRMAGVP
                     IPLAVVQHQLVWTEPLPELAADREAEVVHPILRHQDLSLYFRQQGDRYAVGNYRHEPI
                     LFEPDDLRPYREGAPQPSIEPFTPEHFEVAYEESIRILPPVKDKVDETTAINGMFSFT
                     PDMGSIVGESADVRGFWICEAVWVTHAGGMGRMAAEWIAGGEPSMDMAEADANRFYPF
                     MTTPPYVRARGAQQYREVYDILHPLQQMSHPRELKLTPFHARHEDLGAEFFSGAGWER
                     PQWFEANHVLTGAPWEGRTGWAAQNWSPCVGAEHIATRERVAVFDITPFAKFDITGAD
                     ALSFLERISANRIDRPVGTIVYTSLLTPSGGIRCDLTITRKDEDRFRVVTGGGSGQHD
                     RAWLLGQRRPGERVNLTVRTGSLFALGLWGPKARDVLQAVTDVDVSNEAFPYMTARYL
                     NVGEVDPVWTQRISYAGELGWELYGQIAMGQRAWDVLWEAGEEHGIVAAGGGAFDSLR
                     LEKGYRLWGQDIHTEHDPFDAGLGFAVRMDKGEFQGREALQRKLDRGLTEKLACITLD
                     DPAAVVMGKEPIRHGDRVVSYVTSAAYGYSIGRGIVYGYLPVDLAVEGTLLEVEYFGD
                     RLAATVAAEPLWDPKGERLRA"
     gene            8802..9971
                     /locus_tag="VF195_00410"
     CDS             8802..9971
                     /locus_tag="VF195_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017624225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6579329.1"
                     /translation="MTVIEDPGILLYTRIRKSPYFYASRKHGVARYSVYNHTYHPRHY
                     GDPVAEYWHLVNAVTLWDVGVEKQIQIKGPDAFDLTNMIVPRDLNKCAVDQCKYVFIT
                     GPDGGILNDPVLLRVEDDEFWLSLADSDVNLYALGIAAAKGMDVTVQEIDVAPVQIQG
                     PNAKKVLVDLFGDKILDVPYYGLLKAELDGMQVVVSRTGYTGEVGYEIYLRDASLHGQ
                     QLWDRVLEAGAPHDLRVIGPCHIRRIEGGILAFGADMWYENNPFEVGYHYTWMVDLEQ
                     EADFIGKEALKKIKAEGVMQKLVGVDIEGEPLGTYIDSEMLDFFPASAGGSVVGRVTS
                     ACYSPRMEKNIGLAMLPIEHTELGTSLQVETPTGTVGATVVQTPHWDPTKEIPKG"
     gene            complement(9968..10939)
                     /locus_tag="VF195_00415"
     CDS             complement(9968..10939)
                     /locus_tag="VF195_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579330.1"
                     /translation="MDRSRWRLRTLMPFVLLMACTGPTGDGGSPAPTHPSPVPAIVRT
                     GRIVFGGDDGEIHVLSLSTGEDRTVTSIRGPQFDPDGHGRLIVFRDSRAGVNVNDDIA
                     VISTDGTGYRNLTRTPDANEWGPVWSPDGRRIVYSSDDNGMPQLFVMDADGSNARQLS
                     DVWGEYPAWSPDGSRIAFASSMGGSTPFGDPDYDVFVMNADGSGETNLTTAPDSSEGY
                     PTWSPDGEWIAFGSTRGTPPNFEPPPHDLERRSDEDVWVMRPDGSEPRNLTSDLVRQN
                     KFPDWSNRGLLIDREGSIVILDPDTGAEDDVSERTGVLGVFPAWLER"
     gene            complement(10978..12213)
                     /locus_tag="VF195_00420"
     CDS             complement(10978..12213)
                     /locus_tag="VF195_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6579331.1"
                     /translation="MQAAEVNRGSLAVITATMAFVLVACGGAEEPNNGSPTPPTSGST
                     SPSPTPVPGEAVTFRTSDGVPIAGRLFGEGRVGVVLGHSIDGDQTEWWNFAEVVADAG
                     HAALAIDFRGYCPGAEGGCSGDGNTADAWRDLLAGAEFLRERGVEEIVLAGASMGGTT
                     AIVSAAHARPAVAGVITLSSPIACCGMDVARPAVGAIGAPMLFIAGRFDGDAPRSAKV
                     FARWAGPRGQALILATGEHGTDLFGLATPQVERRTTTAILDFLAGLGGGSVASVFGDW
                     RWVRSCEAFVATFRRADLSDLTTRWLVSARYFAREDQIALDDPCDGAEETPYVYFFEE
                     SGRWGVLDDDDVLVDDRDFSVVDEDTIAFGDVEIDYRIDADELTFDVAIPGACDGTCL
                     EDHAWALATFAPGSFRRVA"
     gene            12258..12803
                     /locus_tag="VF195_00425"
     CDS             12258..12803
                     /locus_tag="VF195_00425"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6579332.1"
                     /translation="MSLRHSLLGLLAEQPRTGYELTRLFDESLLNVWPASHSQIYPEL
                     ARLAADGLIRQTASGARGKKVYSITPDGMEEVLAWLKTEPDHSTRNPSFLRVFFLWLM
                     EPEDAVAFLEREELVHEAKLREFEAMAEQPVRDTSGGWAFRLALDWGVRYEREMLEWN
                     TWARNVIEERRTSAGSARARR"
     gene            12879..13748
                     /locus_tag="VF195_00430"
     CDS             12879..13748
                     /locus_tag="VF195_00430"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6579333.1"
                     /translation="MLQRRRRGTAPTSELEREALIRLVRRPLFELVPLRDALERANAL
                     PAGAATTVTASPSHGIEATIELCEGLRARGHDTTPHLAAHMFLDRAHVGDVLDRCRAA
                     GIRSAFVVGGDAKDRGEIHDGLALIRLMEEVGHPFTSIGVPGYPEGHPSIPDYVLTTS
                     LRDKQAHASYVTTQMSFDPEAISAWIARVRIEGVTMPVHLGVPGAATMRKLTTVGARI
                     GVMGSVRYLRKHRRLLGHVVKRSFGPDALLAALAPALADTTADIRALHLFTFNQVEET
                     VAWQRRMLAELEG"
     gene            complement(13745..14203)
                     /locus_tag="VF195_00435"
     CDS             complement(13745..14203)
                     /locus_tag="VF195_00435"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HEX6579334.1"
                     /translation="MVAEALTPIDLAQVDVRSEPGSLPFDAPEPNTATTWNDRDVLWL
                     GPDEWLVVADPGTVPAIARELDTALTGHPHSVIDVGANRIAFDLTEGLELLATGCGLD
                     LDPSRWRPGMCAQTLLGQAQVILHQRDERTTRVFVRPSFAGYLTQLLASV"
     gene            complement(14193..16673)
                     /locus_tag="VF195_00440"
     CDS             complement(14193..16673)
                     /locus_tag="VF195_00440"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6579335.1"
                     /translation="MTSRRLPEGGTWIDRSKPLTFRFDGVEYEGSEGDTLASALLANG
                     VIGGFRSPILGRPRGVMTAGPEEPNAFVEVSEPWFDPIVAATMVELVDGLVAEPRAGV
                     GRLPAAVVPTRPTEHRHVHVETLIVGAGPLGRAGAAVAAGRGDRVILVDERHRIDDPP
                     DDVTVLPRTTALGLYDDGYVLAFEDTTEFHRLWHVRTGRVILATGATERPIAFPGNDR
                     PGVMLASAAGVYLERFGVLVGDHVTIVTANDSHRELVGVLDAVEAKTEVIDLRGTWAL
                     ELEGGTPLLVAGGWNPNLTLWRAIGGSVRFDSAHACFVPDEGPQWLEVVGAAAGDGLT
                     PTSPVWFLDSDRSTSFVDLQRDQTVGDIAAALDAGLTSVEHVKRATYIGTAIDQGRTS
                     GVLTAAIVNQLLGAGPDAQGPTSARPPAVPLSYAAIAGPDRGDLLDPIRTTRIHRWHE
                     EHGAAFEDVGQWKRARYFPRDGEDMDAAVARECLAVRNAAGLLDASTLGKIEVVGPDA
                     AAFLDRMYTNRMSTLAVGAIRYGFLLGLDGMVADDGVAMRLDEDHFFVTTTTGNAAMV
                     LDRFEEWLQTEWPDLRVYCTSVTEQWADVAVAGPRARDVLSAVDTDLDLLADAFPFMT
                     FRDGDVAGIPARVARVSFTGELSYEILVDGRRALELWEAMVGAGEPFGLQPYGTEAMH
                     VLRAEKGFVIVGQDTDGTVAPDDLGMGWIVRKDASDFIGRRSLTRADTARIGRKQLVG
                     LLPEELLPEGAQLVEEDTGRIPMAMIGHVTSSYRSPTLGRPIALAMVERGRERSGRTV
                     FAPLPGGTISADVVSPVFYDPEGTKHGG"
     gene            complement(16670..16942)
                     /locus_tag="VF195_00445"
     CDS             complement(16670..16942)
                     /locus_tag="VF195_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019973056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX6579336.1"
                     /translation="MLLIRCPWCGSRDETEFHYGGQAHIAYPSDPEALTDEEWADFLF
                     TRDNPRGPFRERWYHVHGCRRWLNALRDTATHRFIATYRIDEEPPA"
     gene            complement(16947..17294)
                     /locus_tag="VF195_00450"
     CDS             complement(16947..17294)
                     /locus_tag="VF195_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007438542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX6579337.1"
                     /translation="MGLRKDTVRGMALALPEATELETWEQPTFRVRKKIFVMFSDGER
                     EAWVKSTRDEQQALVQMDPETFFVPPYVGPSGWVGVRFRTVDRDEMRELVTEAWRMTA
                     PKRLVASFGEDEV"
     gene            complement(17322..18515)
                     /locus_tag="VF195_00455"
     CDS             complement(17322..18515)
                     /locus_tag="VF195_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020515596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit beta family protein"
                     /protein_id="HEX6579338.1"
                     /translation="MRYWRAAEPKPSYDVVIVGGGGHGLATAYSLAKRHGLTDVCVVE
                     KGWLGGGNMARNTTIIRSNYLWDESTAIYEHSLKLWEGLEEELGVDVEFSSRGVLNLA
                     HDLGEVRSSMRRVHANRLNGVDAEWVTPEDVAAFCPLINISPSLRYPVLGATLQRRGG
                     TARHDKVAWAYATRASELGVDIVEHAEVTGFLRGPGGRVEGVSTTRGEIRGAKVALAA
                     AGHTSELASMVGLRLPLQSHPLQALVSALYEPVLDCVVMSNKVHVYVSQADKGELVMG
                     AGIDAYNGYAQRGSFHVIEQQLTAAVELFPIFAHAKLLRTWGGIVDVCPDASPIVGLS
                     GIDGLFLNCGWGTGGFKATPGAGWAYAETIASGEPSELVRPFSLERFTTGALIDEHGA
                     AAVAH"
     gene            18570..19763
                     /locus_tag="VF195_00460"
     CDS             18570..19763
                     /locus_tag="VF195_00460"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6579339.1"
                     /translation="MTPLGLSRDDREIQERTRRFVDEELIPWEVHAEEHEGTIPGEVR
                     DAHVRQARELGLSSMNMPEELGGGGFSMFRQVLVSEQIGRVTNALGWCVHTPPAWAPT
                     VCTPEQIERWISPAVGGERHECYAITEEGAGSDVDAIESTATRDGDDYVLRGTKMHVT
                     SYNTADWIFFQAKLEGGEHEGEHALFFVDKDTPGVRLVREPRYSHTYPDTHAMLEFDD
                     VRVPAANLVGAEGDGMSFTHEWFRYERLMIAARCCGAMERLIDEATAFARERVQFGQP
                     IIEFQAIGHMLADSLTDLWASRLMTYELARGIDAGIDVKIQHAQCSMAKLFASEAAGR
                     VADRAVQIFGGRGYMRENVAERFFRELRVDRIWEGTSEIQRTIVADQLAKRGARRLLG
                     EELGG"
     gene            19760..20215
                     /locus_tag="VF195_00465"
     CDS             19760..20215
                     /locus_tag="VF195_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6579340.1"
                     /translation="MTEVPRTTPETTPEKMPEKMPENMQEGVRDAADRLMAAFSSGDE
                     EGYFACFHPEATFLFHGVDPIKSRAEYRALVRTWKDEHGFQVLSSTSHDADVAVFGDT
                     AVFTHGVTTSQLWDDEEATLHERESIVFQRQPDGAWAAIHEHLSSDEPS"
     gene            20302..21294
                     /gene="speB"
                     /locus_tag="VF195_00470"
     CDS             20302..21294
                     /gene="speB"
                     /locus_tag="VF195_00470"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010509344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6579341.1"
                     /translation="MSNGEPTVQPVSGTVVPRYAGPSTFVRLPELGDLDRCDVAILGI
                     PFDSGVSYRPGARFGPGAIRQASRMLRPQFHPIHEIEPFAFVQGADAGDVACNPFDIK
                     EAIGQIEQGADEVLERAGSIVSIGGDHTVLLPILRSMHRRHGPVALVHFDAHLDTWDT
                     YFGAPYTHGTLLRRASEEGLFLDDASMHVGIRGPLYSSQDMVDDASFGFRTIRTEDVH
                     REGVEGVVQRIRERVRDHPLYISIDIDSLDPAHAPGTGTPEIGGLTSRELLMILRGLV
                     GLHLVSADVVEVCPPYDHADLTSLAAATIVYELVCLLASDPSFASAQTSTTQEG"
     gene            21294..>21503
                     /locus_tag="VF195_00475"
     CDS             21294..>21503
                     /locus_tag="VF195_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013599366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6579342.1"
                     /translation="MFPWPEGKRAALAVTFDMDAEAAVLAVDEKYARRPSIMTHQQYG
                     PVTAVPRLLKLLDTLGIRVSFFIPGF"
BASE COUNT         3462 a         7222 c         7410 g         3409 t
ORIGIN      
        1 gtcctggtcg gtggacaggt agttgaaccg gatcgcgggg tggacccgcg ggtcggtgct
       61 cacgaccttc acggagccgc gggcgtcgga gtacatcggt ccgatgtgca cctggtagcc
      121 gtggccgccg gccggcgccg agccgtcgta ccggatcgcg atcggcagga agtggaacat
      181 cagattcgga tacgccacgt cctcgttgct ccgcacgaat cccccggctt cgaagtgatt
      241 ggtcgcgccc aggccggacc ggaagaagag ccattcggcg ccgatgaagg gcttgcgcca
      301 gttcttcagc cccggcgcca ccgagacggg ttgcgtgcac gcgtgctgga cgtagacctc
      361 caggtggtcc tggagatgtt cacctacgcc ggggagatcc tgcaccaccg ggatccccag
      421 ggaagcgagc tcgcccgcgc tgccgacgcc cgacagctgc agcgtctggg gcgaattgaa
      481 cgccccgccg cagaggatca cctcgccggc gcggacgcgg cggctcggcc cggggccgtg
      541 cgagtagtcc acgcccacgg cgcggatccc ctcgaacagg atccggttca ccatgacgcg
      601 ggtgcggatc tcgaggttcg ggcggctcga gacgggatgg agataggcac gggccgccga
      661 cagtcgacgc ccccgatgca cgttgcggtc gaacggagcg aacccctcct gccggtagcc
      721 gttgacgtcg tcggtcagcg ggtagccggc ctgttgcacc gcctcgaaga acgcgccgaa
      781 cagtgggttc ctggccggcc cccgctcgag gatcagcggg ccgctgtgac ctcggtaggc
      841 gtcggcagga tcggcggcga gacagttctc catccgcttg aagtagggga gacagtgggc
      901 gtggtcccag gtctccatcc ccggatcggc cgcccatcgc tcgtagtcga gcgggttgcc
      961 gcgttggaag atcatcccgt tgatgctgct cgatccaccg agcaccttgc cgcgggcatg
     1021 cgagatccgg cgtcctccca tgttcggctc gggttccgac tcgtatttcc agtcgtacca
     1081 gcgccacccg atcgggtagg tcagggccgc cggcatgtgg atgaagacgt cccacagata
     1141 gtcgggacgc ccggcctcga ggacgaggac tcgcgtggac ggatcggtgg tcaggcggtt
     1201 cgcgagcacg ctccccgccg acccgccgcc gacgatcacg tagtcgaacg cccgatccat
     1261 cgccggaaca gtgtgccgaa aaccgcgacg acgcgctcgc ggaggaactg ggttagcgtg
     1321 ctccctcgtg gacgggtctc ctctcgccgc cgccgatcct ttcgaggtct cggtcggtgt
     1381 cgttcgggag gcgaacgccc gcgggatccc tctcaagctc ctcggcggac aggcggtgcg
     1441 gttgctctgc ccgctcttcc cccaccgggc gcgtgacgat caagacatgg acttcgcctg
     1501 catctcgtcg aagaagcgtg atgtgatcgc gttcttcgcg gaacaagggt tcctcggcga
     1561 ccagcgattc aacaccctcc acggcgaccg gcagatgtac ttcaccaccg cagacgggaa
     1621 gacctcggtg gacgtcgtga tggaccggct caacatgtgt catgtgctcg acttccacga
     1681 ccggatcgac cgactccccg acacgctcga cgtcgccgac ctcttgctca cgaagctcca
     1741 ggtcttcgag ctcaatcgga aggacgtgca cgacatcctc cacctgttct ctggcttccc
     1801 ggtggccgaa ggcgacgagc cgggcacgat cgggacggac agggtgggaa cgctcgtcgg
     1861 cgaggattgg gggtggtggc gcaccgccac gatgaacctg gagaaggtca tcctctactc
     1921 ggaaggtcgg ccgccggacc tcgttcccga ggactccccg ttcgacccct ccgcccaggc
     1981 gaggacgctc ctcggcgtct gcagcgaagc accgaagacc ctgaagtgga agctgcgagc
     2041 caaggtcggg gaccgcgtgc agtggtacca gctccccgag gaggtcgggc actgagcccg
     2101 ccgttgcgtt cgcgggcact ccgcctggct tgcgcacctc ggcgggcggg gtcaagattc
     2161 tctcggtgaa gatcttcttc gtgaccgacc tccacgggtc ggagatctgc tggaagaagt
     2221 tcctgaacgc cggttccttc tacggcgcag acgtcgtcat cctgggcggg gacgtcaccg
     2281 gcaaggcgat ggtcccgatc gtgcagcggc cggacgggac gtgggaggcc tccctccagg
     2341 accaccggga caccctcgag tccgaagacg cgatcgtgga gttcgagaag cgcgtgatga
     2401 accgggggta ctacccgatc cgcgtctccg atcaggagta cgtcgcgatg caggaggacg
     2461 aggacctcgt cgacaaacgg ttcaaggagg tcatgctcga cggcaccgag cgctggatcg
     2521 cgatggcgga ggagaagctc gccgacaccg ggatccgggt gatcgcctgt cccgcgaacg
     2581 acgacatgtt cgagatcgac gatctcctgg cgggggctcg ggtcgtcgag accggtgacg
     2641 aggagcatcc gatccagctc ggaagcttca cgatggtgtc gatgggatgg acgaacccga
     2701 cgccctggaa cacgttccgc gaagcggagg agccggagtt agccacgcgg atcgatcgat
     2761 ctctcgaggc cgccaccgat ccggaaacga cgatcttcaa cttccacgcg ccgccgtacg
     2821 gctcgaagct cgacaacgcg ccggcgctca accccgacct gacgtacgtg tcgggcggtc
     2881 aagcgatccg gccggtcgga tcgacatcgg tccacgaggc gatcaagcgg ttccagccga
     2941 tgctctcgct ccacggccac atccacgaga gcaagggggc gacgaggatc gggaagacgc
     3001 tcgcgctgaa cccagggagc tcgtacgagg aagggatcct gcaagcggcg gtcgtgacca
     3061 tcgacgacaa gaaacggaag gtgaagaact accaactggt gaacggctag agggccgttc
     3121 ggacacctct gggagggggt tgcagatggc ggcggtaggt gaagccccta ccttgttcct
     3181 gcgcaaagcg accggactgg tgaagggatg gtcgaagttc gacgcgttcc tctactcctt
     3241 catgtcggtc aacttcgtca cgctgggtct gttcttcagc cttgcggtca tcgggtacgt
     3301 acctgaaggt caggtgctcc cggcgctggt gatctccggg ctgttcattt cctgcatcgt
     3361 cgttacgtac gccgggctga tctccatcat gccgcgcgcg ggaggcgact acgtgtggca
     3421 gagtcgcgtg ttcgggggcg ggctcgcgtt cgtcctgtcg gtgaccggct ggtggttcat
     3481 cctgtggtac tggacgccga tctacgcgag catcctgaac gtcgagatcc tgcagccact
     3541 ggctgccttc ctcggtgccg acgggctcct cgagttcctc ggtaaagaca tcggcttgtt
     3601 cacgatctgt gtggtgacgg cgctcctcgc tggctacctc gtctccttgg ggatggaggg
     3661 ctatgcgagg atccagaagg tctgcttcta catcggcttg gcggcgctag cagtggtctt
     3721 cgtcgtgatg ctgttcggat ccaaggcggg gttcgaggcg gcgttcaatc aggaggccgg
     3781 cgacctcttc gggacaactg ggaacgcgta cagagacacg atcgcgttcg ccggcggcgg
     3841 cgccgccctc ccagccgata tcgggttgac cccgttcttc gggaagagct tgctgatcat
     3901 cccgttcctg tgcttctgga tcctctggcc gaactggggc tcgacgctgt atggcgaggt
     3961 tcgcggagca agcgacttca agcgcgtgat gagcgggatg atgtggggcc tgtgggtcac
     4021 catcgcgatc gcagccgtgt tcctgttgct ctcgtcgaag ttcttcggat gggacttctt
     4081 caacgcgggg aacgtgaact tcatcaacta cttctacggc tacaccgata gtcctggacc
     4141 cacgcccgtc tggacatatc caccgctgct cgttgggatg ttcttccata acgatctgat
     4201 cggcgcgctc ctcgtgctcg cgttcggcgc gtggttcctc ggttgggcgg gaacgttgtt
     4261 cctgtcgtcg acccgagtga tcttcgcagc agccttcgat cggatcctgc cggagaaggt
     4321 cgcggacgtc tcagagcgcc ggcacgtgcc ggtctgggcg ctgatactga tgctggtgcc
     4381 gtccctgatc gtgagtgcgt tcttcgcgtt cacgacagtg atgaagacct ggatcatcga
     4441 tgcgacgttg gtgatcgccg tgaccttctt cgggacggcg atcgcggcga tgatcctgcc
     4501 ctggcgcaag aagcggttgt acgagagctc tgcgatggct aggtacaaga ttgccggtat
     4561 cccgttgatc accgtgacgg gcgcgctgtc ggctctgttc ctcggctaca acctctacaa
     4621 gtggctctgg ccgccgtaca actcgcctga cgggaatctc tacgcgatca acgtgcccga
     4681 ttcgctctgg ttcatgggct cgatgtactt gctcgccctg ctgatctacg tcgtggcgaa
     4741 gttcgtcagg aggagtcagg ggatcgacct cagtgcgatc cacaaggaga tcccggtcga
     4801 gtaacgccag ggatcggttg gtgcggaggc ggcctttcgg ggtcgcctct cgcgttgcgg
     4861 cgcccgggga tagggtgggt cgatgggcga cgcgccgacg cgggtcttct tcgcggccga
     4921 catccacggt tcgcaggtca cgttccggaa gttcctggcg gccgcggcgt tctactgcgt
     4981 cgacgcgctc gtcttcggcg gcgatctcat gggcaaggcg ctcgtcccga tcctgcgcac
     5041 gaacggcggg taccgggcgt ccttccaggg tcgcgacgag gagttcgacg ccgacgggct
     5101 aggggcgttc accgacgcgc tcgagcagcc cggcttctac tggaaggtct tcgatcgcga
     5161 gcagtacgag gcggtcgaac acgatccgat ggcgaagcac ggcctgttcc acgagctcgc
     5221 ctcggagcga ctggccggat ggatcgaacg tgccgaggag gcgttgcacg gaacgggcgt
     5281 gcgcctctac ctgaccggcg ggaacgacga cgagccgtcg gtgctggaga ccctggatcg
     5341 gcacgacggc gatcgcgtcg tcgcgagcga gggccggctc gtcgacctcg acggggagca
     5401 cacgatgatc acggtcggcg tgtccaccta cacaccctgg gataccccgc gagaggccag
     5461 cgaggaggag atcgcccggg cgatcgagga ggccgtggcg accgtgcccg acgtggggcg
     5521 gtgcgtgttc aacctccact gtccgccgaa ggacactccg atcgacacct gcctgaagct
     5581 cgaggtgcgg ccgggtgagt tgccccggcc gatccgggag ggcgggcggt tcgtgacgac
     5641 cggcggcggg agcacagcgg tccgggaggc gatcgagcgg taccagccgc tggtggggct
     5701 ccacggccac atccacgagt cgggaggccg cttccggatc ggccggacgc agacgttcaa
     5761 ccccgggagc gagtacgtcc agggcgtcct gcagggcgtg atcgtgtcgc ttcggcgcgg
     5821 ccggctggtc tcctaccagc acacgcaggg ctgatcgccg gacgtcctca ggtgttcgcc
     5881 ttcatgagag cgacctggat ctgcggatcg ccgtcaccgc ctgatcggag cacatacctc
     5941 gagcgttccg agacgcgctc ttccccggtc gcatcgaacg cgggccaccg cacgtcgacc
     6001 gaaacgagcc gatcggagag aacctcgatg cgctcgatct ccggacgcgt ttcgacgagg
     6061 ccctgcgagc gatacgcggc gacggcctgc ccgaagaacg cctcgatctc gtcccggctc
     6121 tccaccggga tcgcgccttc atcggagaga accagggccg gcacgaccca acacgcggcg
     6181 gctccgacga ggtcgccgct cccgatcgcg cggccgtacc gctcaaggaa ggtttggatc
     6241 cggccgcgat cctcaggggg caccgaagga gtctagcctg ggccgataca gtcccgcggc
     6301 ggaagcgagg agcgggttgg tgcgggagcg agtgcagaac gtcgtgatcg gcgccggcat
     6361 cgtgggcacg agcgccgcgt atcacctcgc ggagctcggg atcacggacg tgctggtcct
     6421 cgaccagggg ccgctgttcg agacgggcgg gtccacgtcg cacgcgcccg gtctggtgtt
     6481 ccaaacgaac ggctcgcgga cgatgtgtcg gctcgcgcaa tactcggtgg agctgtacgc
     6541 ggggctcgag accggcggtc tgcccgcctg gtacggcgtc gggggcatcg aggtcgccac
     6601 gacccgggag cggatgctgg agctgaggcg gcgccgcggg tacgcgctcg cgtacgggat
     6661 cgaagggacg gagctgctca cgcccgacga gaccgcggcg cggatcccga tcctcgatcc
     6721 cggctccatc ctgggctcct acctcgtccc ctcggacggg atcgcgaaag ggatccgggt
     6781 ggcggctgcg ctcggcgcga acgcgcaggc caagggcgtc gcattcgagg gtggcgtccg
     6841 tgtgacgggg ttcgacatcc gggacggcag aatccacggc gtccggaccg atcggggcga
     6901 catcgagtgc gaccaagtgc tcgtgtgcgc cggcatctgg ggaccgacga tcgggcggat
     6961 ggccggcgtg ccgatcccgc tcgcggtcgt gcagcaccag ctcgtgtgga ccgagccgct
     7021 cccggagctt gcggccgacc gcgaggcgga ggtggtgcat ccgatcctcc gacaccagga
     7081 cctctcgctc tacttccggc agcagggaga tcggtacgcg gtggggaact atcggcacga
     7141 gccgatcctg ttcgagccag acgatctccg gccgtaccgg gagggcgcgc cgcagccgag
     7201 catcgagccg ttcacgcccg aacacttcga ggtggcgtac gaagagagca tccgaatcct
     7261 gcctcccgtg aaggacaagg tggacgagac gaccgccatc aacgggatgt tctcgttcac
     7321 gcccgacatg ggctcgatcg tcggggagtc cgccgacgtc cgcgggttct ggatctgcga
     7381 ggccgtgtgg gtcacgcacg ccggcgggat ggggaggatg gccgccgagt ggatcgccgg
     7441 cggggagccc tcgatggaca tggccgaggc ggacgcgaac cggttctacc cgttcatgac
     7501 gacgccgccc tacgtccgtg cgcgcggcgc gcagcagtac cgcgaggtct acgacatcct
     7561 gcatccgctc cagcagatgt cccatccgcg cgagctgaag ctgacgccgt tccacgcccg
     7621 ccacgaggat ctcggcgccg agttcttctc gggtgccggc tgggagcggc cgcagtggtt
     7681 cgaggcgaac cacgtgctga ccggcgcccc gtgggagggg cggaccgggt gggcggcaca
     7741 gaactggtcg ccgtgcgtcg gggcggagca catcgccacg cgcgagcgcg tggcggtgtt
     7801 cgacatcacg ccgttcgcga aattcgacat caccggcgcg gatgcgctct cgttcctcga
     7861 gcgcatcagc gccaatagga tcgatcggcc cgttgggacg atcgtctaca cgtcgctcct
     7921 gacgccgtcg ggcgggatcc ggtgcgacct cacgatcacc cggaaggacg aggatcgctt
     7981 ccgggtggtc acgggcggcg gctcgggaca gcacgaccgc gcgtggctgc tcgggcagcg
     8041 tcgcccgggg gagcgcgtca acctcaccgt gcgcaccgga tctctgttcg ccctcggtct
     8101 gtggggtccc aaggcccgcg acgtcctcca agcggtcacc gacgtcgacg tctcgaacga
     8161 ggcgttcccg tacatgaccg ctcggtacct gaacgtcggt gaagtcgatc ccgtgtggac
     8221 gcagcggatc tcctacgccg gcgagctggg ctgggagctc tacgggcaga tcgcgatggg
     8281 ccagcgcgcg tgggacgtgc tctgggaggc gggggaggaa cacgggatcg tggcggccgg
     8341 cgggggcgcc ttcgactcgc tccgcctcga gaagggctac cggctgtggg ggcaggacat
     8401 ccacaccgag cacgatccgt tcgatgccgg gctcggcttc gccgtgagga tggacaaggg
     8461 cgagttccag ggccgggagg cgctccagcg caagctggac cgaggactca cggagaagct
     8521 cgcctgcatc accctcgacg acccggccgc cgtcgtgatg gggaaagagc cgatccggca
     8581 cggcgaccga gtcgtctcct acgtgacgag cgccgcgtac ggctactcga tcggccgcgg
     8641 catcgtctat ggctacctcc cggtcgatct cgccgtggaa gggacgctgc ttgaggtgga
     8701 atacttcggc gaccgtctgg cggccaccgt cgccgccgag ccgttgtggg accccaaggg
     8761 cgagcgcctg cgggcctgac tgagaagagg ggaacggaac gatgaccgtg atcgaggacc
     8821 cgggcatcct gctgtacacg aggatccgca agtcgccgta cttctacgcc tcgcggaagc
     8881 acggcgtggc ccgctacagc gtctacaacc acacgtacca cccgcgacac tacggcgatc
     8941 cggtcgccga gtactggcac ctcgtgaacg ccgtcaccct ctgggacgtc ggcgtcgaga
     9001 aacagatcca gatcaagggg ccggacgcgt tcgacctcac gaacatgatc gtgccccgcg
     9061 acctcaacaa gtgcgccgtg gaccagtgca agtacgtgtt catcaccggc cccgacggcg
     9121 ggatcctgaa cgacccggtg ctgctccgcg tcgaggacga cgagttctgg ctctccctcg
     9181 ccgactccga cgtgaacctc tacgcgctcg ggatcgccgc cgcgaagggg atggacgtca
     9241 cggtccagga gatcgacgtc gcgccggtcc agatccaggg gcccaacgcc aagaaggtcc
     9301 tcgtcgacct gttcggcgac aagatcctcg acgttcccta ctacggcctg ctgaaggcgg
     9361 agctcgatgg catgcaggtc gtcgtatcgc gcaccgggta cacgggtgag gtcgggtatg
     9421 agatctacct gcgggacgcc tccctgcacg gccagcagct gtgggaccgc gtgctcgagg
     9481 ccggcgcgcc tcacgacctc cgcgtgatcg gcccgtgcca catccgtcgc atcgagggag
     9541 gcatcttggc cttcggcgcg gacatgtggt acgagaacaa ccccttcgag gtcggctacc
     9601 actacacctg gatggtcgac ctcgagcagg aggccgactt catcggcaag gaagccctca
     9661 agaagatcaa ggccgagggt gtgatgcaga agctcgtcgg cgtcgacatc gagggcgagc
     9721 ccctcgggac ctacatcgac tccgagatgc tggacttctt ccccgcctcc gcaggcggga
     9781 gcgtcgtcgg gcgcgtgacg tcggcgtgct actccccgcg gatggagaag aacatcgggc
     9841 tcgcgatgct cccgatcgag catacggagc tcggcacgtc gctccaggtc gagacgccga
     9901 ccggaacggt cggcgcgacc gtcgtgcaga cgccccactg ggaccccacg aaggagatcc
     9961 cgaagggcta acgctcgagc caggcgggga acaccccgag cacgccggtc cgttcgctga
    10021 cgtcgtcctc cgcgccggta tcagggtcga ggatcacgat cgatccctcg cgatcgatca
    10081 ggaggccccg gttcgaccag tcggggaact tgttctgccg cacgaggtcc gacgtcaggt
    10141 tgcgcggctc ggacccgtcc ggacgcatca cccacacgtc ctcgtcgctc cggcgctcga
    10201 ggtcgtgggg aggcggctcg aagttcggcg gtgtgccccg ggtggatccg aacgcgatcc
    10261 actcaccgtc gggcgaccag gtcggatacc cctcgctgct gtccggcgcg gtcgtgaggt
    10321 tggtctcgcc cgacccgtcg gcgttcatca cgaagacgtc gtagtcggga tcgccgaacg
    10381 gcgtcgagcc tcccatcgaa gaggcgaacg cgatccggga gccgtcgggc gaccatgccg
    10441 gatattcgcc ccagacgtcc gagagctgcc gcgcgttcga gccgtcggca tccatcacga
    10501 agagctgcgg catgccgttg tcgtcggacg agtagacgat ccgtcggccg tcgggggacc
    10561 agaccggtcc ccactcgttc gcatccggcg tgcgagtgag gttccggtaa cccgtgccgt
    10621 cggtgctgat cacggcgatg tcgtcgttga cgttcacgcc ggcgcgcgag tcccggaaga
    10681 cgatgagacg cccgtggccg tccggatcga actgcgggcc gcggatcgac gtgaccgtcc
    10741 gatcctcgcc ggtggacagc gagaggacgt ggatctcgcc gtcgtcgccg ccgaacacga
    10801 tgcgccccgt gcgcacgatc gcggggaccg ggctgggatg cgtcggcgcc ggcgagcctc
    10861 cgtccccggt cggcccggtg caggccatca gcaggacgaa tggcatgagg gttcgcaggc
    10921 gccacctgga tcgatccatg ccgcctgcta caccggcgag cggccggtcg ttccccctca
    10981 ggcgacccgc cggaacgagc cgggggcgaa cgtcgccagg gcccacgcgt ggtcctcgag
    11041 gcaggtgccg tcgcacgctc cggggatcgc gacatcgaag gtgagctcgt ccgcgtcgat
    11101 ccggtagtcg atctcgacgt cgccgaacgc gatcgtgtcc tcgtcgacga ccgagaagtc
    11161 gcggtcgtcg acgaggacgt cgtcgtcatc gagcacgccc catcgccccg actcctcgaa
    11221 gaagtacacg tacggtgtct cctcggcgcc atcgcaggga tcgtccaagg cgatctggtc
    11281 ctcgcgggcg aaatagcggg cactgaccaa ccaccgggtc gtgagatcgg agaggtcggc
    11341 gcgtcggaag gttgccacga acgcttcgca cgatcgcacc catcgccagt ctccgaagac
    11401 cgatgcgacg gagccacctc cgagaccggc aaggaagtcg aggatcgccg tcgtcgtcct
    11461 tcgctcgacc tgcggggtgg cgagcccaaa cagatcggtg ccgtgctcgc cggtcgcgag
    11521 gatcaacgcc tgtccccgtg gacccgccca tcgggcgaac accttcgccg atcgcggcgc
    11581 gtcgccgtcg aagcgtcctg cgatgaagag catcggtgcg ccgatcgcgc cgacggccgg
    11641 ccgcgccaca tccatcccgc agcaggcgat cggtgaggag agggtgatga cacctgcgac
    11701 ggcgggccga gcgtgggccg ccgagacgat cgcggtggtg ccgcccatgc tcgcgccggc
    11761 caggacgatc tcctccacac ctcgttcccg gaggaactcc gcgccggcca gcagatcgcg
    11821 ccacgcgtcg gccgtgttcc cgtcgccgga gcacccgccc tcggcgcccg ggcagtagcc
    11881 gcggaagtcg atcgcgagcg cagcgtggcc cgcgtcagcg accacctcgg cgaagttcca
    11941 ccactcggtc tgatcgccgt cgatcgagtg accgaggacg acaccgacgc ggccctcacc
    12001 gaagagccgg cccgcgatcg ggacgccgtc ggacgtgcgg aacgtgacgg cctcgccggg
    12061 cacgggcgtc ggcgacggcg aggtggatcc gctggttgga ggggtaggtg atccgttgtt
    12121 cggctcctcc gcgcctccac acgcgacgag gacgaacgcc atggtcgccg tgatgaccgc
    12181 gagggatcct cggttcactt cggcagcctg cacctgacac ctttgacatg tcaaggcaga
    12241 caagtaacac tcctcacgtg tcgctccgac attcgctcct cgggctcctc gccgaacagc
    12301 caaggaccgg atacgagctg acgcggctct tcgatgagtc cctcctcaac gtgtggccgg
    12361 ccagccacag ccagatctat cccgagctcg cgaggctcgc cgccgacggc ctgatccgcc
    12421 agaccgcatc gggtgctcga gggaagaagg tctactcgat cacccccgac ggcatggaag
    12481 aggtcctggc gtggctcaag accgagcccg atcactccac gcggaatcca tcgttcctcc
    12541 gcgtgttctt cctgtggctg atggaacccg aggacgcggt cgcgttcctc gaacgcgagg
    12601 agctcgtcca cgaagcgaag ctgcgtgagt tcgaggcgat ggcggaacag ccggttcgcg
    12661 acacatcggg aggttgggcg ttccggctgg ccctggattg gggcgtgcgc tacgagcgcg
    12721 agatgcttga gtggaatacg tgggcacgga acgtgatcga ggagcgacga acatccgctg
    12781 gctccgcacg agcgcgccga tgagcggctg acctcgggtc gcgacccgtc tttgcctcgt
    12841 gaaccgggtg tgcgtcacga tgccgcatgc cgatgaggat gctgcagcgc agacgccggg
    12901 ggaccgcgcc cacgtctgag ctcgagcgcg aggcgctcat ccggctcgtg cgacggccgc
    12961 tgttcgagct cgtccccctc cgcgacgcgc tcgagcgagc gaacgcgctg ccggcaggtg
    13021 ctgcgaccac ggtgacggca tcgcccagtc acgggatcga ggcgacgatc gagctctgcg
    13081 aggggctgcg tgcccggggt catgacacca ccccgcatct cgcggcgcac atgttcctcg
    13141 accgcgccca cgtcggcgat gtcctggacc gttgcagggc cgcggggatc cgcagcgcct
    13201 tcgtcgtcgg tggcgacgcg aaggaccgag gggagatcca cgacgggctg gcgctgatca
    13261 ggctgatgga ggaggtgggc cacccgttca cgtcgatcgg ggtcccgggg tatccggagg
    13321 ggcatccctc gatcccggac tatgtgctga ctacctccct ccgggacaag caggcgcacg
    13381 cgtcctacgt gaccacgcag atgagcttcg atccggaggc gatctcggcc tggatcgcga
    13441 gggtgcgcat cgaaggggtc acgatgccgg tgcacctcgg cgtccccgga gcggcgacga
    13501 tgcggaagct gacgaccgtg ggcgcgagga tcggcgtgat gggctccgtg cggtacctcc
    13561 ggaaacatcg gcgcctcctc ggccacgtcg tgaagcgatc gttcgggccc gatgccctcc
    13621 tcgcagcgct cgcccccgcg ttggccgaca cgacggccga catccgggcg ctccacctct
    13681 tcacgttcaa ccaggtcgag gagaccgtgg cctggcagcg tcggatgctg gccgagctcg
    13741 agggttagac ggatgcgagc aactgggtga ggtagccggc gaacgacggg cggacgaaga
    13801 cacgcgtcgt tcgctcgtcg cgctggtgga ggatcacctg ggcctggccg aggagcgttt
    13861 gcgcgcacat cccggggcgc cagcggctgg gatcgaggtc gaggccgcaa ccggttgcga
    13921 gcagctcgag gccttcggtg aggtcgaacg cgatccggtt cgcgccgacg tcgatgacgg
    13981 agtgagggtg gcctgtgagc gcggtgtcga gctcgcgggc gatcgccggc acggtgccgg
    14041 ggtcggcgac gacgagccac tcgtccggcc cgagccacag gacgtcgcgg tcgttccacg
    14101 tcgtcgcggt gttgggctcg ggtgcgtcga aggggagcga ccccggctcc gagcgcacgt
    14161 cgacctgcgc cagatcgatc ggggtgaggg cctcagccac catgtttcgt accctcgggg
    14221 tcgtagaaga cgggggacac gacgtccgcc gagatcgtgc cgcccggaag cggcgcgaat
    14281 acggtccggc cggagcgctc gcgtccgcgc tcgaccatcg ccagcgcgat cggacgccca
    14341 agggtgggac tccggtacga cgacgtgacg tgcccgatca tcgccatcgg gatccgaccg
    14401 gtgtcctcct cgacgagctg cgccccctcc gggaggagct cctcgggcag cagcccgacg
    14461 agctgcttcc gaccgatccg ggccgtatcc gcacgagtga gcgatcgacg cccgatgaag
    14521 tccgacgcgt ccttccgcac gatccatccc atcccgaggt cgtccggtgc cacggtgccg
    14581 tcggtgtcct ggccgacgat cacgaagccc ttctcggctc gcaggacgtg catcgcctcc
    14641 gtcccgtatg gctggaggcc gaacggctcg cccgcgccca ccatcgcctc ccagagctcg
    14701 agcgcgcgtc gaccgtccac gaggatctcg taggagagct cacccgtgaa gctcactcgg
    14761 gcgacccgcg ccgggatccc cgcgacgtcg ccgtcacgga acgtcatgaa cgggaatgca
    14821 tcggccaaga gatcgagatc ggtgtccaca gccgagagca cgtcacgcgc tcgcggaccc
    14881 gccacggcca cgtcggccca ctgctcggtg acgcttgtgc agtacacgcg caggtccggc
    14941 cactcggtct ggagccactc ctcgaaccgg tcgaggacca tcgcggcatt gcccgtcgtc
    15001 gtcgtgacga agaagtggtc ctcgtcgagg cgcatggcca cgccgtcgtc ggcgaccatc
    15061 ccatcgaggc cgagcaggaa cccgtagcgg atcgcgccga cggcgagcgt gctcatccgg
    15121 ttcgtgtaca tccggtcgag gaaggccgcc gcatccggcc cgacgacctc gatcttcccg
    15181 agcgtcgagg cgtcgagcag gccagcagcg ttgcgcacgg cgagacactc acgggcgacc
    15241 gccgcgtcca tgtcctcgcc gtcgcgcggg aagtagcgag ctcgcttcca ttgacccacg
    15301 tcctcgaagg ccgcgccgtg ctcttcgtgc caccgatgga tgcgggtcgt gcggatcggg
    15361 tcgaggagat cccctcgatc cggccccgcg atcgccgcgt acgacagggg gacggcgggg
    15421 gggcgggcgc tcgtcgggcc ctgcgcgtcg ggcccggcgc ccaggagctg attcacgatc
    15481 gctgccgtca ggacgccgct cgtccttccc tgatcgatcg cggtgccgat gtacgttgcc
    15541 cgcttcacgt gctccacaga cgtgagtccg gcgtcgagcg ccgccgcgat gtcgccgacg
    15601 gtctggtcgc gctggaggtc gacgaacgag gtcgagcgat ccgagtcgag gaaccacacc
    15661 ggcgacgttg gggtcaggcc gtcgccggcc gctgcgccga cgacctcgag ccactgcgga
    15721 ccttcgtccg ggacgaagca ggcatgcgcc gagtcgaacc ggacggaccc gccgatggca
    15781 cgccacagcg tcaggttcgg gttccatccc cccgcgacga gcagtggggt cccgccctcg
    15841 agctcgaggg cccaagtccc gcgtaggtcg atgacctctg tctttgcttc gaccgcgtcg
    15901 aggacgccca cgagctcacg atgcgagtcg ttcgcggtca cgatcgtcac gtggtcgccg
    15961 acgaggacgc cgaaccgctc cagatacacc ccggcggccg atgcgagcat cacgcccggg
    16021 cggtcgttcc cggggaaggc gatcgggcgc tcggtcgcgc cggtcgcgag gatcacgcga
    16081 ccggtgcgca cgtgccagag tcgatggaac tcggttgtgt cttcgaacgc caggacgtac
    16141 ccgtcgtcgt agaggccgag cgccgtcgtt cgcggaagca cggtgacgtc gtcgggcggg
    16201 tcgtcgatcc gatggcgctc gtccaccagg atcacgcggt cgccgcgacc tgccgcgacg
    16261 gcggcgcccg cccgccccaa ggggccggcc cccacgatca acgtctcgac atgcacatgg
    16321 cggtgctcgg tcggccgggt cggcacgacc gccgcgggca gacgcccaac gcccgcccgc
    16381 ggctccgcta cgagcccgtc gacgagctcg accatcgtcg cggcgacgat cgggtcgaac
    16441 cacggctcgc tcacctcgac gaacgcgttc ggctcctccg gccccgcggt catcacgccg
    16501 cggggccggc cgaggatcgg gctccggaag ccgccgatca caccgttcgc aagcagggcg
    16561 ctcgcgagcg tgtcgccctc ggatccctcg tactcgaccc cgtcgaaccg gaacgtgagc
    16621 ggcttcgatc ggtcgatcca ggtgccgcct tccggcagcc ttcgtgacgt catgcgggcg
    16681 gctcctcgtc gatccggtag gtcgcgatga accggtgcgt cgcggtatcc cggagggcgt
    16741 tgagccatcg tcggcatccg tgcacgtggt accagcgctc gcggaacggc ccccgcgggt
    16801 tgtcccgcgt gaacaggaag tcggcccact cctcgtcggt cagggcctca gggtcggatg
    16861 ggtacgcgat gtgcgcctgg cccccatagt ggaactcggt ttcgtcgcgc gagccgcacc
    16921 agggacaccg gatcaggagc atcgggctac acctcgtcct cgccgaacga ggccacgagc
    16981 cgcttcgggg cggtcatccg ccacgcctcg gtgacgagct cgcgcatctc gtcccgatcc
    17041 acggtccgga atcgcacgcc cacccagccg ctcggtccaa cgtacggcgg gacgaagaac
    17101 gtttcggggt ccatctggac gagagcctgc tgctcgtctc gggtgctctt gacccacgcc
    17161 tcgcgctctc cgtcggagaa catcacgaag atcttcttgc gcacccggaa ggtgggctgt
    17221 tcccaggtct ccagctccgt cgcttccggt agcgcgagcg ccatccctcg cacggtgtcc
    17281 ttgcgcaagc ccatctcagt gcgcttccat atccgcgcgc atcagtgcgc gaccgccgcg
    17341 gcgccgtgct cgtcgatcag cgcgccggtc gtgaaccgct cgagcgagaa cggccgcacg
    17401 agctccgacg gctcaccgct cgcgatcgtc tccgcgtagg cccagccggc ccccggggtc
    17461 gccttgaacc cgccggtgcc ccacccgcag ttcaggaaca gcccgtcgat gcccgagagc
    17521 ccgacgatcg gcgacgcgtc cggacacacg tcgacgatgc ctccccaggt ccggagcagc
    17581 ttcgcatggg cgaagatcgg gaacagctcg accgccgcag tgagctgctg ctcgatcacg
    17641 tggaacgacc cgcgctgcgc gtagccgttg tacgcgtcga tcccggcgcc catcacgagc
    17701 tcgcccttgt cggcttgcga cacgtagacg tgcaccttgt tcgacatcac gacgcagtcg
    17761 aggaccggct cgtacagggc gctcaccaac gcctggagag ggtgcgactg cagcggtagc
    17821 cgcagcccca ccatcgaggc gagctcgctg gtgtgccccg cggccgcgag cgcgaccttc
    17881 gccccccgga tctcgccgcg cgtcgtcgac acgccctcca cgcgaccgcc gggtcctcgg
    17941 aggaatcccg tgacctcggc gtgctcgacg atgtccacgc cgagctcact cgctctcgtc
    18001 gcgtaggccc acgccacctt gtcgtgccgg gccgtccccc cgcgtcgttg gagcgtcgcc
    18061 ccgaggacgg ggtaccgcaa cgacggcgag atgttgatca acggacagaa cgcagcgacg
    18121 tcctcgggcg tgacccattc ggcgtcgacc ccgttcaggc ggttggcgtg gacgcgcctc
    18181 atcgacgacc gcacctcgcc gaggtcgtgc gcgaggttca ggacaccgcg agatgagaac
    18241 tcgacgtcga cgccgagctc ctcctcgagg ccttcccaga gcttcaggga gtgctcgtag
    18301 atcgccgtgg attcgtccca gaggtagttc gatcggatga tcgtggtgtt gcgcgccatg
    18361 ttgccgcccc cgagccagcc cttctcgacg acgcagacat cggtgaggcc gtggcgcttc
    18421 gcgagggagt aggcggtggc gaggccgtgg ccgcctccgc ccacgatcac gacgtcgtag
    18481 gaaggtttcg gctcggcggc ccgccagtag cgcacggcgc acatcttgcc gcagcggcac
    18541 cggggccgtc cgccgtatgc tcagcggcca tgacgccgct gggcctctcc cgagacgatc
    18601 gcgagatcca ggagcgcacc cgtcgattcg tcgacgagga gctgatcccc tgggaggtcc
    18661 atgccgagga gcacgaggga acgatccccg gcgaggtccg cgacgcgcac gtccggcagg
    18721 ccagggagct gggtctgtcc tcgatgaaca tgccggagga gctcggcggc ggcggattca
    18781 gcatgttccg gcaggtcctc gtctcggagc agatcggccg ggtcacgaac gcgctcggct
    18841 ggtgcgtcca cacgccgccg gcgtgggccc ccaccgtctg cacgcccgag cagatcgagc
    18901 ggtggatctc gcccgcggtg gggggggagc ggcacgagtg ctacgcgatc accgaggagg
    18961 gcgcgggctc ggacgtcgac gcgatcgagt cgacggcgac gcgcgacggc gatgactacg
    19021 tcctgcgcgg cacgaagatg cacgtgacct cctacaacac ggccgactgg atcttcttcc
    19081 aggcgaagct cgaaggaggc gagcacgagg gggagcacgc gctgttcttc gtcgacaagg
    19141 acacgccggg cgtgcggctg gtgcgcgagc cgcgctattc ccacacctat cccgacaccc
    19201 acgcgatgct cgagttcgat gacgtgcggg tgcccgccgc gaacctggtg ggcgccgagg
    19261 gggacgggat gtcgttcacg cacgagtggt tccggtacga gcggctgatg atcgcagcgc
    19321 gctgctgcgg agcgatggag cgactgatcg acgaggccac cgcgttcgcg agggagcggg
    19381 tgcagttcgg acagccgatc atcgagttcc aggcgatcgg ccacatgctg gcggactccc
    19441 tcaccgacct gtgggcgtcg cgactcatga cctacgagct cgcccggggg atcgatgccg
    19501 ggatcgacgt caagatccag cacgcccagt gctcgatggc caagctcttt gcatcggagg
    19561 ccgccggacg ggtcgccgac cgcgccgtcc agatcttcgg cggccgcggc tacatgcggg
    19621 agaacgtggc cgaacgcttc ttccgggagc tgcgcgtcga ccggatctgg gagggcacgt
    19681 cggagatcca gcgcaccatc gtggccgatc agctcgcgaa gcgcggcgcc cgccgcttgc
    19741 tgggagagga gctcggagga tgaccgaagt gccccgcacg acgccagaga cgacgccgga
    19801 gaagatgcca gagaagatgc cagagaacat gcaggaaggg gtacgggacg cggcggatcg
    19861 gctgatggcg gctttctcgt ccggggacga ggagggctac ttcgcgtgct tccaccccga
    19921 ggccaccttc ctgttccacg gagtggatcc gatcaagtca cgtgccgagt accgggcctt
    19981 ggtccgcacg tggaaggacg agcacggctt ccaggtgctg tcgtcgacgt cgcacgacgc
    20041 ggacgtcgcc gtcttcggag acaccgccgt gttcacgcac ggtgtcacga cgtcccaact
    20101 gtgggacgac gaggaggcga cgctccacga acgcgagtcg atcgtgttcc agcgacagcc
    20161 ggatggcgcg tgggccgcca tccatgagca cctctcatcg gacgaaccct cctagctccg
    20221 gggccggagg tcggcgcaac tcgacgccga gccgggccgg gcgctagcat ccgcgggcgg
    20281 tcatccaggg gagggcgcga catctcgaac ggcgagccca cggtccagcc cgtcagcggc
    20341 accgtcgtgc cccggtacgc agggccgtcc acgttcgtcc gactccccga gctgggcgac
    20401 ctcgatcgat gcgacgtcgc gatcctcggg atcccgttcg actccggtgt gagctaccgc
    20461 cccggcgctc gcttcggtcc cggcgcgatc cgccaggcct cccggatgct tcggccacag
    20521 ttccacccga tccacgagat cgagccgttc gccttcgtgc agggcgccga cgccggcgat
    20581 gtcgcctgca accccttcga catcaaggag gcgatcgggc agatcgagca gggggcggat
    20641 gaggtgctcg agcgagccgg ctcgatcgtg agcatcgggg gtgaccacac ggtcctgctg
    20701 ccgatcctgc gctcgatgca ccggaggcac ggtcccgtcg cgctcgtgca tttcgatgcg
    20761 cacctcgaca cgtgggacac gtacttcggc gctccgtaca cccacggcac gctcctgcgc
    20821 cgcgcgtcgg aggaggggct gttcctcgat gacgcctcga tgcacgtggg catccgtggt
    20881 cccctctaca gctcgcagga catggtcgac gacgcgagct tcggcttccg gacgatccgc
    20941 accgaggacg tccaccgcga gggggtggaa ggggtcgtcc agaggatccg cgagcgggtt
    21001 cgggatcacc cgctctacat ctcgatcgac atcgactcac tcgatccggc tcacgccccc
    21061 ggaacgggga cgccggagat cggtgggctg acgtcgcgcg agctgttgat gatcctccgc
    21121 ggtctggtgg gcctccatct cgtgtctgca gacgtggtcg aggtttgccc gccctacgac
    21181 cacgccgacc tcacgtcgct cgctgccgcg accatcgtgt acgagctcgt gtgcctgctc
    21241 gcgagcgatc cgtcgttcgc gagcgcccag acgagcacca cgcaggaggg ctgatgttcc
    21301 cctggcccga ggggaagcgc gcggcgctcg cggtcacctt cgacatggat gcagaggcgg
    21361 ccgtcctcgc cgtcgacgag aagtacgccc ggcgcccctc gatcatgacc catcagcagt
    21421 acggacccgt gacggcggtc cctcgcctcc tgaagctgtt ggacacgctc gggatccggg
    21481 tcagcttctt catcccgggc ttc
//
LOCUS       DASUYD010000010         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1108495, whole genome shotgun sequence.
ACCESSION   DASUYD010000010 DASUYD010000000
VERSION     DASUYD010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108495"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..368
                     /locus_tag="VF195_00480"
     CDS             84..368
                     /locus_tag="VF195_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579343.1"
                     /translation="MKIKGTCRRCGREFLAEQVIRNGGRCPWDGKPFQADYAVVLVDV
                     LTDAEAAGNTLENALEKLADIEPEFVLDEGTVLDEIQGHLERLERVHGGT"
     gene            373..639
                     /locus_tag="VF195_00485"
     CDS             373..639
                     /locus_tag="VF195_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579344.1"
                     /translation="MTSAPNLDAGNRYTVVVRDEAAGQGMGQGTGQEKGWIVTILDPD
                     GREVSERACRDETEAITFASTVRQHAGWLSEGRFREIYRIPEGA"
     gene            749..1105
                     /locus_tag="VF195_00490"
     CDS             749..1105
                     /locus_tag="VF195_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6579345.1"
                     /translation="MTNEELGALDYCYLTTKGRRSGSPHTIEIWFALRGEAVYLLAGG
                     GDASDWVKNVRSDPTVGLRLGDRDLICKARLVGDPEEDALARRLLLEKYRHRYSGELE
                     EWARTSRAVAIDLPRD"
     gene            complement(1107..2068)
                     /locus_tag="VF195_00495"
                     /pseudo
     CDS             complement(1107..2068)
                     /locus_tag="VF195_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(2157..>2712)
                     /locus_tag="VF195_00500"
     CDS             complement(2157..>2712)
                     /locus_tag="VF195_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX6579346.1"
                     /translation="ATPLAGSSQRFGDAGRDRASADRLFDSDKDREEHAVVVEDVHHV
                     LDSFCEDLVHPHEPELLGTANVWHLATPLRGRLHDPSVTALELVAALHPTPAVCGSPR
                     ETALEALEHLEPIERGGYAGPVGWMDANGDGEWAIALRCAEITGSTARLYAGAGIVAD
                     SEPEAELDETERKFRALLDALRWG"
BASE COUNT          462 a          891 c          949 g          410 t
ORIGIN      
        1 gagctcgcgg accggctggt cgagctgatc gagagcgaca ggcaccacca aaggaaccag
       61 cggaaggatc agagggagac cgtgtgaaga tcaaggggac gtgcaggcgg tgcggccgag
      121 agttcctggc cgagcaggtc atcaggaacg ggggacgctg tccgtgggac ggcaaaccgt
      181 tccaggcgga ctacgcggtg gtgctcgtgg acgtgctcac cgacgccgag gcggcgggca
      241 acaccttgga gaacgcgctc gagaagctcg ccgacatcga gcccgagttc gtgctcgacg
      301 agggcaccgt gctcgacgag atccagggtc acctcgaacg gctcgaacgc gtccacggcg
      361 gcacgtgaga ccgtgacgag cgccccgaac ctcgacgcgg gcaatcgcta caccgtcgtc
      421 gtccgcgacg aggcagccgg ccaggggatg ggccagggaa cgggccagga aaaaggatgg
      481 atcgtgacga tcctcgaccc ggacggacgc gaggtgtcag agcgagcctg ccgcgacgaa
      541 accgaagcga tcacgttcgc atcgaccgtt cgccagcacg ccggctggtt gtccgagggg
      601 cggttccgcg agatctaccg gatccccgaa ggggcatgac atgggcttca acacgatgca
      661 gccgggtaag gtcaagcgcg gcgacctcgt gttcttgctg gtcggggtgg cggtgatcgt
      721 cgccctgatc gtgtgggccg tttcgtaggt gaccaacgag gagctggggg cgctcgacta
      781 ctgctacctc accacgaagg gccggcgttc gggcagccca cacacgatcg agatctggtt
      841 cgcgctgcgc ggcgaagctg tctacctgct cgccggcggc ggggacgcaa gcgactgggt
      901 gaagaacgtt cgcagcgatc cgacggtcgg ccttcggctc ggcgaccgag acctgatctg
      961 caaggcccgc ctcgtgggcg atcccgagga agacgcgctc gcgcgacggt tgctgctcga
     1021 gaagtaccgg caccgctact cgggggagct ggaggagtgg gctcggacct cgcgtgcggt
     1081 cgcgatcgat ctgccgcggg actgagctag gtgtagttcc cacctacgtt gtctgcactc
     1141 gcgagatcgg cgagagccca cccagcgcgg tgtgaggtct gctccgattg taggtgtgga
     1201 tccagggcgg cagggctgcg agcctcgctc cgttggagcg gtagaggcgt gaataagccc
     1261 actcgtcgag catggtgcgg ttgaaccgct ccgccttgcc attggtctgg ggacggtaag
     1321 ggcgtgtcac cacgtgtcgg gccccgatct cggacaacgc atcggcgaac accctcgaga
     1381 gcacgtagtt cttcgcctgg tcggtcatga cccgctcgac ccgcacccca agatcggcca
     1441 gatggacagc ggcccgcagc acgaagcccg cgcaggtatc gcctcgttcg tcggagtgga
     1501 cctcgacgaa cgcgtaacgg gagtgatcgt cgatcgcgac gtgcaggtag tcatagcctg
     1561 caccgcgacc tttccccgcc ttggactcgc tgctttgccc gagcttccgc caccctccac
     1621 cggggcggat gcgcccgagc ttcttcacgt cgatgtgcag gagctcccct ggatgctcac
     1681 gctcgtagcg gaccgggaca cccgtaggac ggtccagatg cgaaagccgt gatatgccgc
     1741 gccgacgcag gaccgcatag accgtggaac gcggatgccc gagctcggga ccgaggcggt
     1801 gcggtccgac cttcaggcga cgccgtgccc ggcagatccg gtcgacctca cgctctgaca
     1861 gggcgtgcgg tcgtcggtgg ggagcggagc ttcgcccttc gaggccttcc aggccttcct
     1921 cacggaagcg tcggagccac ttgtgtgccg ttgagcgcga cactccgagg gactccgcgg
     1981 ctgccgagac ggaccagccc tcctgcaaga tccgcgtgac gagcagatac cgcccgaagg
     2041 gcgtgagcct cgccctacga tggcccatga gagcctcctt ccggctaggg actcggacaa
     2101 gccccaaacc gtgcagggag gctctcgcct gtcaacaacc ctgatgggaa ctacacctag
     2161 ccccagcgga gcgcgtcgag cagcgcccgg aacttgcgct cggtctcgtc gagctccgcc
     2221 tctggctccg agtcggcgac gatgcccgcc cccgcgtaca acctcgccgt cgagccggtg
     2281 atctccgcgc atcggagcgc gatcgcccac tcgccgtctc cgttcgcgtc catccatccg
     2341 acgggtccgg cgtacccgcc ccgctcgatc ggctcgaggt gctcgagcgc ttcaagggcg
     2401 gtctcgcgcg gcgaaccgca gacggcgggc gtggggtgga gggcggcgac gagctcgagc
     2461 gccgtgaccg acgggtcgtg caaccggccg cggagcgggg tggccaggtg ccacacgttg
     2521 gcggtaccta ggagctccgg ctcgtgcgga tgcacgaggt cctcgcagaa cgagtcgagc
     2581 acgtggtgga cgtcctcgac cacgacggcg tgctcctcgc gatccttgtc cgaatcgaac
     2641 agccggtcgg cggacgcgcg gtcgcgtccc gcatcgccga accgctgcga cgagccggcc
     2701 agcggcgtcg cc
//
LOCUS       DASUYD010000011         5744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1549836, whole genome shotgun sequence.
ACCESSION   DASUYD010000011 DASUYD010000000
VERSION     DASUYD010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5744
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1549836"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VF195_00505"
     CDS             complement(<1..353)
                     /locus_tag="VF195_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6579347.1"
                     /translation="MSHLGTIPPERSELAEALGTVEGAMTKSVVSVSRDMSASEALAY
                     LIRHGVGGAPVVESDRVVGVVTTSDLAAPHPYARETGPFLRPHSGGSEWQVRDLMTES
                     PVTASPDEPLVDAVV"
     gene            644..1075
                     /locus_tag="VF195_00510"
     CDS             644..1075
                     /locus_tag="VF195_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579348.1"
                     /translation="MDRYVRNVLLVESDRVERERLAAALEGDGFEVVLCTGPTAPDYT
                     CVGARVGRCPLATDGCVVVIDMDQDGDAAVSGTSAEELLDFYLQAQHPVVTLSARPIA
                     RDDARVLELRHHPETDTLLTAAWWAASPKATGHPVVRGAWS"
     gene            1201..1599
                     /locus_tag="VF195_00515"
     CDS             1201..1599
                     /locus_tag="VF195_00515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6579349.1"
                     /translation="MFEKILLAVDGSEHSNRATAAASELAKSGGSVHVLHVHEAGLVL
                     PVETNTEAQGVVDQAIKQLEADGVAVYGQAVSARAGSVAPAIIDAARAFGCDLIVIGT
                     RGLSDFSGLLVGSIAHKVIHHADCPVLVVR"
     gene            1839..4550
                     /locus_tag="VF195_00520"
     CDS             1839..4550
                     /locus_tag="VF195_00520"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6579350.1"
                     /translation="MATGAFQPLESDVVLRDGSTVRIRPARPSDQTRVEDYLIGLSPE
                     TRRLRFWSQAIDVRGLARKIVDVDPENHLTLLVLHGGDEGRMIGGAQFIRTHRARAEI
                     SLSVTDEFQGRGIGSILLGQLAEAAAAHDIATFSADVLPENHPMINVFRSSGFDLSIR
                     ATPGAIEVEFPIGLTDTAIQHFEDRETQAAISAMRTFLTPRSVAVIGASRDSSAIGGR
                     LFHNLLMAELSGSVFPVNPQADVVHGVVAYPNVEAIPGDVDVAFVAVPAIHVVEIAQQ
                     CGRKGVWGLVVISAGFAEVGDEGVQRQARLLKTCRSYGMRLIGPNCMGIVNTAEDVRL
                     NGTFANVYPPAGRVGFSSQSGALGLAVMQYAAELGLGLSSFVSVGNKADISGNDLVAY
                     WSVDERTDVILLYLESFGNPRRFARLAREIGRSKPIIAVKSGRSAAGQRAAVSHTGAL
                     LATSDVTVDALFRQAGVIRTDTLEEMFDVATILANQPPPRGDRVAIVTNAGGLGILCA
                     DACEAHRLHVATLSEQTMAALRSFLSTEASLGNPVDMIASATASDYRRTIEIVAADPA
                     VDAMIVIYIPPEATKAEEIGEAIAEGIDALEGRIPVATAWMSTKGLPAELQKTGTRIP
                     SFAFPEQAAIAMARAAAYGRWREKHVGSVKTFDDARPDEAYAILATALGRRDDGWLSP
                     DEVERLLECYGLRIARSERATTPEEAGEAAARIGGRVAVKAFGPDIVHKTEIGGVVLG
                     LSGADEVTRAAREIADRMAAAGISVEGFLVQEMVEGGVEMLVGVAHDPLFGPVVAVGA
                     GGTIVELLKDVSVRITPISDLDAQEMVRSLGTFPLLDGFRGAPKADVAALEEVILRIG
                     ALAENHPSVAEMDCNPVKVLPSGAVIVDARVRVEEPPPTKPLAGLGG"
     gene            complement(4567..>5744)
                     /locus_tag="VF195_00525"
     CDS             complement(4567..>5744)
                     /locus_tag="VF195_00525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6579351.1"
                     /translation="LHEVRRMDDGDVLALREAGPDRVAYLDNLKVLMVAGVIAGHAIA
                     GYAGMNWTYADVAEGEMGVLSQSVFGLLILPFAVFVMALFFLVAGLLTPGSLDRKGPG
                     RFARDRLVRLGIPLAAFTLVLWPLMTYGLYRAAGSDWSLWDVYADDPTLDNGPLWFVE
                     ALLIFSLAYAGWRTWRMRRPDNWRKTSGLLRPRSLVVLGAMIAIATFITRLWFPFDSL
                     QFTNAHVWQWPECIGLFGLGIVSARRGWLRPVPDRIRRGCGWVAMIATAALVLAFAST
                     QDPDRSVDAFGGGWRWEAFGLAIIGGAMSVAASIWVLALVQRRWDQAGRTARAMARSA
                     YAAFMLQGVPLILFALALRAFDVPLEIKALVVAVGGIAGSFGLGWLLVSRTRLGRIL"
BASE COUNT          978 a         1929 c         1986 g          851 t
ORIGIN      
        1 ctgaccactg catcgacgag cggctcgtcg ggagaggccg tgacggggct ctccgtcatc
       61 agatccctca cctgccactc ggaccccccg ctgtgtgggc gaaggaaggg gcccgtctcc
      121 cgtgcgtacg gatgcggagc cgcgaggtcg gaggtcgtga cgacccccac gacgcgatca
      181 ctctcgacca cgggcgctcc accgacaccg tgtcgaatca ggtatgccaa ggcctcgctc
      241 gcagacatgt cgcgcgacac ggacacgact gacttcgtca tcgcgccctc gaccgtgccg
      301 agggcctcgg cgagctcgga tcgctcaggc ggaatcgtcc ccagatgaga catgtcacca
      361 cctccgggct caggctggca gaccgcgaag ccgcgggatc gggccgttgg accccctgca
      421 cacggaccat cggccccgag gacatcacgg cgacgcccga cgcgcgggag cagggcgtcg
      481 gagataggca ggttcctcac gccctcgggc gtggagacca acgacgccac aggccgccta
      541 gcgccgggtc ggatggcccg tcgaggacgg gctcgtcgac cctcggcgcg gacaagccgg
      601 tgcgggacgc ttccgggaac gacacgcaga gtaaggaggc gtcatggacc gatacgtgag
      661 gaacgtgctc ctcgttgagt ccgatcgcgt ggagcgggaa cgcctcgccg cggcgctcga
      721 gggtgacggc ttcgaggtcg tgctgtgcac cgggcccacg gcgcccgatt acacctgcgt
      781 cggagcgagg gtcggcaggt gtccgctggc cacggatggg tgcgtcgtcg tgatcgatat
      841 ggatcaggac ggcgacgccg ccgtctcggg aacgtcggcc gaggagctgc tcgacttcta
      901 cctccaggcc cagcaccccg tcgtcacgct gagcgctcgg ccgatcgcca gagacgacgc
      961 gcgcgtcttg gagctgcggc accacccgga gaccgacacg ttgctgacgg ccgcgtggtg
     1021 ggcggcgtcc ccgaaggcga cgggacatcc ggtggtgaga ggcgcctgga gctgaaccca
     1081 ccgatcggtc ccgtcgatct cgggaccttc gcctcctgac gtccgctccc ctcgggtgag
     1141 acacacgggt tggctgtacc cgtgggccgc caatcggtcc ccggaggaaa ggaggaggag
     1201 atgttcgaga agattctctt ggcggtggac ggctcggagc actccaaccg ggcgacggcc
     1261 gcggcctccg agctcgcgaa gtccggaggc agcgtccacg tgttgcacgt ccacgaggcg
     1321 ggcttggtcc tgcccgtgga gacgaacaca gaagcccagg gtgtggtgga ccaggcgatc
     1381 aagcagctcg aagccgacgg cgtcgcggtg tacggacaag ccgtgtcggc tcgggccggt
     1441 tcggtcgccc ccgccatcat cgacgcggct cgtgcgttcg gctgtgacct gatcgtaatc
     1501 ggaaccaggg gtctgtccga tttctcgggc ctgctggtcg ggagcatcgc tcacaaggtc
     1561 atccaccacg ctgactgccc cgtcctcgtg gtgcgctaac acggttagcg cggatcgcag
     1621 ggcgctccgt caggtgcagg gcggctccgc cacgtgtagc ggcggagatt gcggcgatcg
     1681 gaaccctccg ctatcgggga gggcgatcgt cctccgaccg cttcgaccgt aagggccccc
     1741 gaagcgcccg tgacctcgcc gaacgtcacg tcccgcccag gactttcggc caaggtgctg
     1801 accccgcccg cctgccacgc ttcactacga tggatgcgat ggcgaccggg gcgttccaac
     1861 cactcgagtc ggacgtcgtt ctgcgggacg gatccacggt tcggatccga cccgcccgac
     1921 cgagcgacca gacccgcgtc gaggactatc tgatcgggct ctcacccgag acgcgccgcc
     1981 tccgcttctg gtcccaggcg atcgacgtcc gaggactcgc ccgcaagatc gtcgacgtcg
     2041 atccggagaa ccatctgacc ctgctcgtcc tccatggggg ggatgaggga cggatgatcg
     2101 gtggtgccca gttcatccgg acacaccgcg cgcgcgcgga gatcagcctc tcggtgaccg
     2161 acgagttcca aggtcgaggg atcgggtcga tcctgctcgg ccagctagcc gaggccgccg
     2221 cggcccatga catcgccacc ttctcggccg acgtcctgcc ggagaaccac ccgatgatca
     2281 acgtcttccg ttcgagcggc ttcgacctgt cgatccgggc gactccggga gcgatcgagg
     2341 tggagttccc gatcggcctg acggacacgg cgatccagca cttcgaggat cgtgagacgc
     2401 aggcggcgat cagcgcgatg cggacgttcc tgacgccacg ttcggtcgcc gtgatcggag
     2461 cgtcgcgaga ctcctcggcc atcggaggcc ggttgttcca taacctcctg atggccgagc
     2521 tctccggctc ggtcttcccg gtgaatccgc aggcggacgt ggtccacggt gtcgtcgcgt
     2581 acccgaacgt cgaagcgatc ccgggcgacg tcgacgtcgc gttcgtcgcg gttcccgcga
     2641 tccacgttgt agagatcgcg cagcagtgcg gccgcaaggg cgtgtggggg ctggtcgtca
     2701 tctcggccgg attcgcggaa gtgggcgacg agggcgttca gaggcaagcg aggctcttga
     2761 agacctgccg ctcgtacggc atgcgcctga tcggtccgaa ctgcatgggg atcgtgaaca
     2821 cagccgagga cgtgcggctg aacgggacat tcgcgaacgt gtacccgccg gccggacggg
     2881 tcggcttctc gtcgcagtcg ggggcgctcg gcctggcggt catgcagtac gcagccgagc
     2941 tcgggctggg cctgtcctcg ttcgtctccg tgggcaacaa ggccgacatc tccggaaacg
     3001 acctcgtcgc gtactggtcg gtcgacgagc gcacggacgt gatcttgctg tacctcgagt
     3061 ccttcgggaa cccgaggagg ttcgcgcggc tcgcccggga gatcggaagg agcaagccga
     3121 tcatcgccgt gaagtccggc cgcagcgccg caggccaacg agccgccgtc tcccacaccg
     3181 gcgcgctcct tgcaacctcc gacgtcaccg tcgacgcgct gttccggcag gcgggcgtga
     3241 tccggacgga cacgctggag gagatgttcg acgtcgcgac gatcctggca aaccagcccc
     3301 cgccgagggg cgaccgcgtg gcgatcgtca ccaacgcggg aggcctcggc atcctgtgcg
     3361 ccgacgcatg cgaggcccac cggctgcatg tcgcgacgct ctcggagcag accatggcgg
     3421 ccttgcgatc gttcctttcc accgaggcta gcttggggaa cccagtggac atgatcgcct
     3481 ccgcgacggc ctcggactat cgacgcacga tcgagatcgt ggcggccgac cccgctgtcg
     3541 acgcgatgat cgtgatctac atcccacccg aggcgacgaa ggccgaagag atcggtgagg
     3601 cgattgccga gggtatcgac gcgctcgagg ggcggatccc cgtggccacg gcatggatgt
     3661 ccacgaaagg actaccggcg gagcttcaga agaccgggac ccggatcccg tcgttcgcgt
     3721 tcccggagca ggcggcgatc gcgatggcgc gcgcagcggc gtacggccga tggcgcgaga
     3781 agcatgtggg gtccgtcaag acgtttgacg acgccagacc ggacgaggcg tacgccatcc
     3841 tcgcgacggc gctcggccgc cgagacgacg gatggctgag cccggacgaa gtggaacgcc
     3901 tcctcgagtg ctacgggctg aggatcgcgc gttcggaacg ggcgacgacg cccgaggaag
     3961 cgggggaggc agcggcccgg atcgggggca gggtcgccgt caaggcgttc ggcccggaca
     4021 tcgtgcacaa gaccgagatc ggaggtgttg tgctcggact gtcaggggcc gatgaggtta
     4081 ctcgcgcggc tcgggagatc gccgatcgaa tggcagccgc cgggatctcc gtcgaggggt
     4141 tcctcgtcca ggagatggtg gaaggcggcg tcgagatgct ggtgggcgtg gctcacgacc
     4201 cgttgttcgg gccggtggtc gcggtgggcg cgggcggaac catcgtggag ctgctcaagg
     4261 acgtctccgt ccgcatcacc ccgatcagcg atctggacgc gcaggagatg gttcgatccc
     4321 tcggcacctt cccgctcttg gatggctttc gaggagcgcc caaggccgac gtagccgcgc
     4381 tggaggaggt catcctgagg atcggcgccc tcgctgagaa tcatccgtcg gtcgccgaga
     4441 tggactgtaa tcccgtaaag gtcctgccgt ccggcgcggt gatcgtcgat gctcgagtga
     4501 gggtcgagga accgccgccc acgaagccgc tcgccgggct cggcggctga taggacccac
     4561 tcctcgttag aggatccggc cgaggcgagt ccggctgacg aggagccagc cgagaccgaa
     4621 cgagcccgcg atcccgccga cagcgacgac cagcgccttg atctcgagcg gcacatcgaa
     4681 cgcccgcagg gcgagggcga acaggatgag cgggaccccc tggagcatga acgcggcgta
     4741 ggccgaccgg gccatcgctc gagcggttct gcctgcctgg tcccatcgcc gctggaccaa
     4801 cgcgaggacc cagatcgacg ccgcgaccga catcgcccct ccgatgatcg cgagcccgaa
     4861 cgcctcccag cgccatccgc cgccgaatgc gtccaccgac cgatcggggt cctgggtcga
     4921 ggcgaaggcc aagacgaggg ccgctgtggc gatcatcgcc acccagccgc agcctcgccg
     4981 gatccggtcc gggaccggcc ggagccatcc gcgccgtgcg ctcacgatcc cgaggccgaa
     5041 caggccgatg cattcgggcc actgccagac gtgcgcgttc gtgaactgca aggagtcgaa
     5101 ggggaaccac agtcgggtga tgaacgtggc gatggcgatc atcgcaccga ggaccacgag
     5161 cgagcgcggt cggagcaggc ctgaagtctt cctccagttg tcgggccgcc gcatccgcca
     5221 ggtcctccag cccgcatatg cgagcgagaa gatcagcagg gcctccacga accacagggg
     5281 gccgttgtcc agcgtcgggt cgtccgcgta cacgtcccac aggctccagt cggatcccgc
     5341 cgcgcggtac agcccgtacg tcatcaaggg ccacagcacg agcgtgaagg ccgccaacgg
     5401 gatccccaga cgcacgagcc ggtcgcgagc gaagcggccg ggacctttgc ggtcgagcga
     5461 acccggcgtg agcaggcccg ccaccaggaa gaacaaggcc atcacgaaca ccgcgaacgg
     5521 gaggatcagc agcccgaaca ccgactgcga tagaactccc atctcaccct cggccacgtc
     5581 ggcgtaggtc caattcatcc ccgcgtatcc agcgatcgcg tgtccggcga tcacgccggc
     5641 aaccatgagc accttgaggt tgtcgaggta cgcgacccgg tccgggccag cctcacggag
     5701 cgcgagcaca tcgccgtcgt ccatcctgcg tacctcgtgc aacg
//
LOCUS       DASUYD010000012        31614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_889212, whole genome shotgun sequence.
ACCESSION   DASUYD010000012 DASUYD010000000
VERSION     DASUYD010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 31614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31614
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_889212"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2183)
                     /locus_tag="VF195_00530"
     CDS             complement(<1..2183)
                     /locus_tag="VF195_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX6579352.1"
                     /translation="MAISTGRRSITVPGLIAADPFLERYWVRSGGAAVVALDGDDQLT
                     VRDPDGGQVAEITALAPDGSDDAAALGARADVPASVLRSLIGSEAEGASDVIGSLAGR
                     GLNPSDATAVRLFGEWSPAGSSQTFTAERSVVVVVAAPAGVSVLEGGVPGSDLDVEIR
                     RTRPRTHVELELPPPLAEPRLDFRVDRATALSYEVKAGEYIQIIDVEGKQCSDFLAFH
                     RGKLEGGTERGFDPTITRTLMGNAFPQPGLYSKFYDLDMDVLVEVIRDTVGRHDSFLL
                     ACTSRYYENLGYFGHTNCTDNFNRQLETYAIAPRKGWQALNFFYNTMFATDNLLVFDE
                     PWSRPGDYVLLRAATDLVCASSACPDDVDPANGWDPTEVHVRVYSAEHTFSAAIAHRV
                     TPDADPTLTTETGFHPRISELTSRTTEYNGYWLPASFDNHGATDEYWACRERAAIMDL
                     SPLRKFEVLGPDAEALLQATVTRDVRRLSDGHVVYTAMCNEDGGMIDDATIFRIADTN
                     FRFVGGAEYDGIWLREQASRLGLHAFVKESTDHLHNVAVQGPASRELLKEIVWVPPAQ
                     TPFEGLGWFRFSVGRLGGPTGVPAVISRTGYTGELGYEIFCHPKDALTVWDAVWGAGE
                     KHGLAPLGLDALDILRIEAGLIFAGSEFDDQVDPFEAGIGFTVTEGKDEDFVGGEALA
                     ERRAHPQRALVGLELDGNEPAAHGDGVYAGRQQVGVVTSGTRSPIL"
     gene            complement(2189..3592)
                     /locus_tag="VF195_00535"
     CDS             complement(2189..3592)
                     /locus_tag="VF195_00535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017317118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6579353.1"
                     /translation="MTDKMWGGEPFWGLGYDWDPDWVYTDKQKELRATLIELCEKELR
                     ANAKRFDDELMFPRRNLELLGEHGFLALTVPEEYGGLGENHVAYSMVCETIARYGCAS
                     TAMCYVMHIGAVNAIMLRATPELIDKYIRPLNSGKIGTLSYSDPETGSHFWYPISSGA
                     ERSNGGFKVRKKASWTTSGGFADFYVVQTTSPDFSGYDDLSVFVVDGEHIQAQPSLWD
                     ALGLRGNQSGPIEIDNVEIPAEQLVGPTGDGANSNDESVDPWFLVGSASVWNGLALGA
                     IDIAKRHTTRKKHVDVGMRVADYPTIQDYVGEAVMDTNASRLFTLSVAQAMDGATENN
                     TVNPGPGELARGNFLHWAWQIKFEAAKNTAHVVDKMLHACGGSGYKRDMELERYLRDA
                     KAGWVMGPTNEVLRQFVGKAVLLGFESLDYWNQSYNRRAVENEVKKLDAAGKRELAEQ
                     LFKEADEQTSKEPAAKG"
     gene            complement(3712..5238)
                     /locus_tag="VF195_00540"
     CDS             complement(3712..5238)
                     /locus_tag="VF195_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6579354.1"
                     /translation="MADEVTAQATIEERHLLKSLNWYDGFVIGLCQPAFLLGSLGFTL
                     ATIGAWGSILLWGISATVGLLQNFIYAETAAMFPDKPGGIALYAHEGWRSRFNLFGPI
                     AAFGYWIGWSVVLSFVGNIVGALIVAEWFPKSGDGVDDAYFNLGFADFGLAQAIAIIV
                     ILAVWLFNVLGVKPSLAVGYVTGALLVVPLAIFIIFPYLTGDWSSSNLEYVHNAGGFS
                     DLRIALMWLFIMCWSAYGIEVGASFAPEYHDTKRDTSKALKSVAVFSLFVYLFLPLGL
                     GGVVGSVGDYGSFYVAALEEIAGSALGGFAVICLIASLILAMNTSTADGGRALYGIAR
                     DDMTIRWLYHLNKFHVPARGMTVDMVVNIGLLLLLGANNFVILYISNIGYVFCHVLAL
                     TGFLLLRRDRPDWPRPIKAGPVFTALAWPLAAFNLVLVLAGVIDQKVASYIGFYEFKG
                     LPLFLGAGVLVASVALFFYRRAVQDRKPITFRDPDVPTMPSENQMRLLREEVMPEPSS
                     "
     gene            5366..5722
                     /locus_tag="VF195_00545"
     CDS             5366..5722
                     /locus_tag="VF195_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579355.1"
                     /translation="MKGPPITVRCDCGEVESVPYGEAWTCPTCGRRWDTAQIPSDEYW
                     RVMREMRRYRLEVIAVSVVLGIALAITLANLGSRRYFPIVLGVMGFWFLIYMPRWRQK
                     VRRRVRELPGWRLRSG"
     gene            5798..6091
                     /locus_tag="VF195_00550"
     CDS             5798..6091
                     /locus_tag="VF195_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX6579356.1"
                     /translation="MTFLITCPNCGPREALEFSFGGEATRRAGPTATDRQLAAALYFR
                     RNVDGWQTEWWVHQSGCRAWFLAERHTTTNEIRRTYRPQERPRGEEDREAAPA"
     gene            6088..8898
                     /locus_tag="VF195_00555"
     CDS             6088..8898
                     /locus_tag="VF195_00555"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6579357.1"
                     /translation="MSERLPARQGEAIRRDRPITFRFERRTIAAFEGDTVGSALGAAG
                     VTITGRSFKYHRPRGLSCMTGSCPNCLMQIDGIPNVRACTEPARDGMKVERQNAWPSA
                     DRDIHGWLNTFSFMMPPGFYYKIFQRPRWAWPLVEPFIRSKAGLGKVPHEPDHRRRRR
                     INLHPDVLVIGAGAAGLAATAEAAKAGASVVLLEQGREAGGHLLGEASSTLRSGLARE
                     ALGSGARFLPETAAFGVFGGGLIAAAGPDALYRIRARHVIFATGAVEGSAVFPGNDLP
                     GVMLSSAVHLLLHRYGVLPGRRAVVLTGDNVGYATAWALKDAGAEVTVVDLRSDGGWP
                     EGFPVFAGSTILSAHGRRRVSAVSVGTVGATSGQTVSCDLVVNACVQTPSTNLLAQAG
                     ARLAFDATLQAFLPAEIPARAWAVGAVAGARSPEASVAQGRLAGLEAAAGSGHQVDTA
                     SMAALRSEAVRSGDPIVLPPEASAASGKQFACICMDVTAKELETAVAEGFDSMELLKR
                     YTTITMGPCQGKACMASSQRLCGAVTGRSFADTSPTTSRPPWVPVELGTLAGARLTPR
                     KETTMHDRHVAAGAEFMWAGDWRRPHHYTGPEAEVAAVRHRVGLIDVSTLGTFRIRGP
                     EAVELLERLYPNRYSDLAVGRVRYGAMLNDEGVILDDGAVVRLADDEFFVTVTTGNTA
                     ALERWITWWNADWGLDALVLNVTGAYAAVNLAGPRARAVMASLTDTDVSPAAMPYMTA
                     TRMAVADVPSLVLRIGFVGETGYEIHFPSTYGDHLWDAVMEAGRSEGIVAFGLEAQRI
                     LRLEKQHILVGQDTDAESDPYEAGLGWMVKDDKEDFLGLRSLRDLKDRGPRERLVGFT
                     SSERWLPPEGASVVHEGAWVGRVTSARRSEAVGGVVGLAWVPADWAADGRTFEIQFGG
                     SHATGTVRTDPFYDPRGERLRS"
     gene            8895..9521
                     /locus_tag="VF195_00560"
     CDS             8895..9521
                     /locus_tag="VF195_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579358.1"
                     /translation="MSTAIRRSPAARLSSRRGAVFVEEAGWEIPASYGNDAGERAAIR
                     DRVAIVDITARAKVDIRGRIPETLPSTADTVLARISAEWAVLLGAPDAEGRLIRAIEP
                     VAGAGAMVTDVTHLFAGVALAGPALPELIERATSWDLSTLPAGAATGAPIAEVRALLV
                     RRGLPFPVLEVFVAMEFGRYVWETLASIVSSLGGAPAGWQALRAEGWR"
     gene            9521..10735
                     /locus_tag="VF195_00565"
     CDS             9521..10735
                     /locus_tag="VF195_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009630763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6579359.1"
                     /translation="MHPIRYFRPWRMWRRHERKDAYDVVIVGAGAHGLAIAYELAKRG
                     IRDVAVLDKSYIGAGGSGRNTTIVRANYRTPEGVAFYKESLRLYEQLAQELDYNLLLS
                     QQGHLTLAHAERAVIVAHERAEVNRLLGVDSRVIYPDEIAKLCPELDLSTHPDFPIMA
                     ALYHPPGGVIRHDAVVWAYARMADRMGVHIHQDTEVTGIDVRHGRVTGVRTNRGDIAA
                     GTVISAVAGWTSEVCRMAGVETPIVTHPLQAFVTEPLKPFLDVILVSATLHVYISQTD
                     RGEVLIGSEIEPYTSYSQESTLDFLEVTAAHSLELLPMLGRVKLMRAWGGLCDVTPDY
                     SPIMGLTDVEGFLIDGGWGTYGFKATPIVGVTMADLIADNRVPDLIAPFALERFWKRE
                     LVSEIAAAAVSH"
     gene            10900..11766
                     /gene="fdhD"
                     /locus_tag="VF195_00570"
     CDS             10900..11766
                     /gene="fdhD"
                     /locus_tag="VF195_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEX6579360.1"
                     /translation="MMATAAQPLRRNTSSVHVVAMNDGVRSERTDTLAAEEPLEIRVQ
                     GPGQDLRSVAVTMRTPGGDFELAVGFLFTEGLIAPGDVSRVAYCDNLPGEDQQYNVVS
                     VTLDRPFDFDLLKRNFYATSSCGVCGKAALDDIVVRCAPVATGTMVTIETLLGLPDAL
                     RKVQKVFDRTGGLHAAGLFTSEGQPISVREDVGRHNAVDKAVGEQLLAGGMPLADHVL
                     QVSGRLSFEIVQKAAMAGIPIVSAVSAPTSLAVEAGERFGMTLVGFVRDERLNVYTHP
                     ERVGGSERGDEA"
     gene            complement(11763..12557)
                     /locus_tag="VF195_00575"
     CDS             complement(11763..12557)
                     /locus_tag="VF195_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX6579361.1"
                     /translation="MTERAPRQPRVGMLDRLRGSTGSRITPHPGPVGAEQDASSSKSG
                     ALRAAIFGMNDGLVSNLSLIFGVAGSGVAADVVVIAGFAGLLAGAFSMGSGEYISMMV
                     QREVFEQLIHKEAHEIATQPEEELRELAEIYERKGISKETAARIAAEVMEDPEVALET
                     HAREELGIDIKEGLGSPWAAAGSSFVMFSIGAVAPLLPFLFTSGKTAIAVSASLSAVT
                     LFAVGAAMTVLTGRGVLVSGARMLGIGAVAAAITYGVGNLVGVAVS"
     gene            12687..14609
                     /locus_tag="VF195_00580"
     CDS             12687..14609
                     /locus_tag="VF195_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:12933..12935,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6579362.1"
                     /translation="MPNGLTRLTEPMVREDGELRVATWEEALDRAADGLRAATERHGG
                     TGVGLFSCSKATNEMNFAAQKFFRQALGSNNIDSCNRTUHAPSVVGLATVFGAGGGTS
                     SYDEVEHTDLMLLWGSNARAAHPIYFHHVLKGIRKGMRLIVVDPRRTESAEWGDLWLG
                     LDVGTDIALSNTMAREIIVNHLHHRAFIELATTDFEDYAASVMDWTLERGAEVTGIPA
                     QVIREVALAFAKADRAMICWTLGITEHHNAADNVLALINLGLLTGHVGTYGSGLNPLR
                     GQNNVQGGGDMGAIPHHLTGFQEVENDAVRTRFEQEWGAKINPKKGLHLSQMFEAMEE
                     GELTALYVIGENPAASEADNQRTHKLLSNLDILIVQDIFLTQTAKMADVVLPAANSAF
                     ESEGTVTNSERRVQRVRPALVPPGNAKDDIWIISQLADRLGRDWGDPSAHDMWEEVRR
                     LSPMHAGMAWERLEELGGIQWPCPDEEHPGTTFLHGRLWSEDPEAQGRKAPFSVVLYE
                     PPVDELDEDYPFRLTTGRRLDSYNTGVQTGEYTSPLRREETLDISPEDAERLGFREGE
                     RVRVLSRRGEVVAPVRYALGLRPGLLFMTLHFPDQVDVNNLTIDAWDPKSGTAEFKAT
                     AVRVEKLPAAIASPGG"
     gene            14620..16482
                     /locus_tag="VF195_00585"
     CDS             14620..16482
                     /locus_tag="VF195_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003994173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX6579363.1"
                     /translation="MDLHLMDAAPSEAERAAVDGLLGPPETGWVGATERSAADLRVVR
                     AGRQAREQRHLLLPALHALQAGVGWISRGGLNYVCQRLSVPPADAYGVATFYAMFSVE
                     ERPANVVHVCDDLACRGAGGLALCGELERDAGPEGVDAGGWTWVRSPCLGMCEQAPAL
                     FAQRVGRPEVALGGATRATVDGIVSPDSAVVCAGGVGAPQTADARSRDSLRLLRRAGV
                     VDPTSIDDYRAHEGYGALRAAHEMGRGSVIEAVTEAKLLGRGGAAFPTGVKWQAVSTQ
                     PAGPRYVVCNADESEPGTFKDRVVMEQDPFALVEALTIAGFACGAEKGFIYVRGEYPL
                     ATERLEHAIGEARRHGALGHDVMGSETAFDIELRRGAGAYICGEETALFNSLEGKRGE
                     PRNKPPFPVTFGLFGRPTAINNVETLINVLEVLRIGADAYAAIGTEGSTGPRLFCLSG
                     AVERPGVYEVEHGATVGDLLALAGGVRQGSALKAVLLGGAAGGFVGPDMLDARLTFED
                     ARQGGYTLGSGVIMVFDERADLVDLCLRIARFFRDESCGQCVPCRVGTVRQEEALHRL
                     VAGTPIGSTGDELALIDEVARVMRDASICGLGQTAANAVQSAISKLSVFDGGAA"
     gene            16479..17507
                     /locus_tag="VF195_00590"
     CDS             16479..17507
                     /locus_tag="VF195_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017593953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6579364.1"
                     /translation="MSRAVPLDPPRRLLDLEIDGRTTRVPEGATILDACRAEGVDTPT
                     MCYAGNLTPVNACRVCMVEVEGSRVLVPACSRPVEDGMKVQTDTERVRHSRKLVMEFL
                     ASSVDTSLASTDWLRWMGEYGADPSRYGAPMDPMEGGERDERWAGHHHDPDPAIAETV
                     AQPVKVDNELYVRDYERCVLCYKCVEACGVDAQNTFAIAVAGRGFDARISTEFAVPLD
                     ESACVYCGNCIGVCPTGALMFKSEHDMRAEETWDEEAQTVTNTICPYCGVGCELEVHV
                     QDNEIVKVTSPEDHSVTSGHLCIKGRFGFAYVQRRGDRRGSDRGGGAPIPSPTAGGEG
                     ATPENPGS"
     gene            17504..18136
                     /locus_tag="VF195_00595"
     CDS             17504..18136
                     /locus_tag="VF195_00595"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX6579365.1"
                     /translation="MRSTGGPGEPEAPIGGIVLAGGRSRRFGRDKLAEQIDGRPLLHH
                     PILRLLDVCDRVVVVIGADVEEPPMPVGASVTFARDSTADEGPLRGLAAGLEIAHATW
                     AVVAGGDMPDLQPPVLREMLRAARETAAVAVVLSDGGEARPLPCVVRTEPAADAVAVL
                     LGSGRRRLGDLLSAVRTVVVDEPSWAALDPGRRTLVDIDEPADVERAPRR"
     gene            18146..18967
                     /locus_tag="VF195_00600"
     CDS             18146..18967
                     /locus_tag="VF195_00600"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6579366.1"
                     /translation="MPHEPFMLGSVEVVPLCDGWAPLPLDDEAPGRDVDWDEQRTRFP
                     WAFPPDDATSWAWHVHSFLIRHPDGPVLVDTGIGGLGRPPFDVAGRLEDELATIEVSP
                     ESVRHVMLTHLHGDHAGGACAADGGPLFPNARYHVHPDDWTFFATHRTPEDLTSRFAM
                     AGLEGLGMLDLDPTDHEVMAGLSVRHAPGHTPGHRVIQLEDGTRTLLLVGDLLHTTPQ
                     VAHPTWLSNHDEDPELAAEHRALWIDAADREGWTVCVGHFGRPFGHVAAAGWSPV"
     gene            complement(18978..20690)
                     /locus_tag="VF195_00605"
     CDS             complement(18978..20690)
                     /locus_tag="VF195_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579367.1"
                     /translation="MSEDRSENGSTVKRVLADVGTSFSALGRSFRSPDISKALAAYLA
                     FTITEWAAFIALIVYAYQDGGSVMVGLVSVLQLIPAALIAPVGSVLGDRHRRERVLVY
                     SYASLGAMTMLAGVALLTDAPAPLVYASGTTAGWVLTLVRPTHASLLPRLARTPEELS
                     SAFAASSLLESFSILLGPLLAGGLMGLTPGSVSGPGVVDATLAAMLLLGTVSVASIRI
                     RTEPAHDGGTSGARIVASDAVDGVRAVARDRRSLLLVSTMGLAMVQLGFVDVLIVVLA
                     FDVIGTSDAGVGFLTASIGVGAVVGAMLAIGVAARWRASRSFRWGISWSGLSIAGIAS
                     QPALAGVFLAMSGAGGALADVNGRVMLQRLIPDKQLSRAFGVLESLYMAGEGIGSFLA
                     SLIIVAIGPRWTLLIAGVLLPSVALLGRRALIALDVGIRIPSEEMALLRSTRFFAPLP
                     TPMLERLARNLIPIHVPDGSVVITQGEAGDRFYAIIEGGVEVLERGRRVTTLGPGGSF
                     GEIALIRDVPRTATVVATTESKLLALDRDEFLRAITGHDAAAEAAHGLATERLDELEA
                     SREP"
     gene            20810..22120
                     /locus_tag="VF195_00610"
     CDS             20810..22120
                     /locus_tag="VF195_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HEX6579368.1"
                     /translation="MSRRVLIMGAAGRDFHNFNVVFRDDPSTEVVAFTATQIPYIDDR
                     TYPAGLAGERYPDGIRIHEEDELSRLIRQLEVDDVVFAYSDVSHEYVMHKASEALAAG
                     ANFLLLGPDATMLKPAIPTVAVTAVRTGVGKSQTTRAVAATLKGAGKKVVAVRHPMPY
                     GDLTAQRVQRFAELEDLDRHHCTIEEREEYEPHITTGTVIYAGVDYAAILEQAQSEGD
                     VLLWDGGNNDLPFYLPDVHIALVDPLRVGHETRYHPGEANLRRADVILINKMDSSTPE
                     HVARLEETIAQMNPMATVVKANSKVTVDEPGAIDGKRVLVIEDGPTLTHGEMKIGAGV
                     VAAQRSGAAEIVDPRPWAVGTIAETFEKYDVGAVLPAMGYSQGQLDEMAKIIDAADAD
                     LVVIGTPIDLRHVIDIRKPAVRVRYDLDVLPDSPQLLDVLRPVL"
     gene            22164..23102
                     /gene="arcC"
                     /locus_tag="VF195_00615"
     CDS             22164..23102
                     /gene="arcC"
                     /locus_tag="VF195_00615"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011843102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEX6579369.1"
                     /translation="MERVVVALGGNALLRRGADDTFEELYRAARAAAERLAEIAAEGW
                     EVVVTHGNGPQVGRILIQQEAAAKTVSPMPLDVCGAESQGLIGYLLQVTIGDVFFERG
                     MERPVATVLTLTRVRSQDPAFRKPSKFIGPFYTAAQARKLEEQRHWVMKEDPHGGWRR
                     VVPSPRPYSIVETPIVKRLVADGVIVIAGGGGGVPVIEKGPRLIGKEGVVDKDLAAAI
                     LAAEVEASTLLILTDVQKVQRGFGTLYPDAIDRMTADEAADLLERGEFGAGSMGPKVQ
                     AAIDFLEAGGTRVMIGDLDEAPAVLRGEAGTEIVPS"
     gene            23099..23803
                     /locus_tag="VF195_00620"
     CDS             23099..23803
                     /locus_tag="VF195_00620"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6579370.1"
                     /translation="MKGATLRVATWNVHGMRAGVDAIARVIRAEHIDIVVLQESGPRR
                     RLRAVGETLGMLVCADPWVFPRRRIQNAVLVRRGLATTVSHRLHRFSGGSLLYPRGVL
                     LADVDHRVSVLSVHLGLSRSERAHHVDQLLAMLEGSAGTFVVGGDLNALPGDPGPSSL
                     AGLASDCWEAAGEGAGATYASHAPTARIDYLFADRAFRPLRAWTAGGTASDHLMVVAE
                     LALTESGEDGASVRGS"
     gene            complement(23760..23906)
                     /locus_tag="VF195_00625"
     CDS             complement(23760..23906)
                     /locus_tag="VF195_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579371.1"
                     /translation="MLAINGFLEWFFLGLLSFLVAAVTLFGLYMAIQLFRSPGRSPRP
                     PRSL"
     gene            23959..24987
                     /locus_tag="VF195_00630"
     CDS             23959..24987
                     /locus_tag="VF195_00630"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX6579372.1"
                     /translation="MRLGIITGGGDCPGLNAVIRAVVRRGIRAHGMEFVGFHDGWHGV
                     IDDVSQPLGIADTTGILPRGGTILGSSGANPYRFEDGPARVLATIAKHALDGLVVIGG
                     EGSLGVAVRLADEGVRIVGVPKTIDNDLSGTDVTVGFHTAVQVATDAIDRLHSTAESH
                     DRVMLVEVMGRHAGWIAVYAGMAGGADAILIPEHPFDVDGVCHHIRHRHASRSSFSIV
                     VVAEGAVPIEGTIELPEPPLDENGFPRLGGLANHLAPEIEKRTGFQTRVTILGHVQRG
                     GSPVSFDRVLATRFGIGAADLAAAGGWGRMVARKAQEVRDVPIADAVTERNVVPLELY
                     REAEVFFG"
     gene            25018..26166
                     /locus_tag="VF195_00635"
     CDS             25018..26166
                     /locus_tag="VF195_00635"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX6579373.1"
                     /translation="MRIGILTGGGDVPGLNACIKAATTRAVGDGHEMVGIRRGWAGLL
                     ELDPADPSSTSTHTLALDPDVVRTIDRTGGTILHTSRTNPAKVRAADEPDFLKAGRVN
                     DEARDHTSHVMKVLDHLGIDAMIPIGGDDTLSFALRLHDEGVPVVAVPKTMDNDVHGT
                     DYCIGFSTAVTRTVIFIHQLRTSAGSHERLAVVEVFGRYSGETSLVSAYLSGMDRAII
                     SEVPFDVEKLATFLMDDKRKNPSRYAMTTISEGAHFVGGEMVMGGTADAYGHRKLGGI
                     GAQTGEALKEITGEGIIYQQIGYLMRSGQPDSLDLMVAINYAVMAADLLLEGTHGRLV
                     ALRNGSYVNVPLEVVRQGVKRADVDALYDADEYRPKIRHVDGKPMFLY"
     gene            26216..27271
                     /locus_tag="VF195_00640"
     CDS             26216..27271
                     /locus_tag="VF195_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018467711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEX6579374.1"
                     /translation="MISLRVDLRSDTVTKPSEAMRRAMAEAEVGDDWYDDDPTVNLLQ
                     ERCAEAVGKEAGAFVPTGTMANQIGLRLHFTGPGHLVAAAAGLHVTSVEVMTAAALAG
                     IAYRTIEAGPRGWIDAEQAAHLLEPDEFYDVEVIDLLSVENTVGGAGGRVMPIEELRA
                     VRKVANDAGVPIHLDGARIFNAAAAAEVDAADIAAEADTVMFCLSKGLGAPIGSVLCG
                     PADMAREARRLKILYGGAWRQAGIVAAAGLIALEEGPKRLHEDHERARQLAAGVAEAF
                     PGSVDLEQVETNMVLVDTEAVGVPMLEAIERLAALGVGTTHSGGKIRMVTHLDVDDTG
                     VAVALDAWRELASERGR"
     gene            27271..27894
                     /locus_tag="VF195_00645"
     CDS             27271..27894
                     /locus_tag="VF195_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015794626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HEX6579375.1"
                     /translation="MALFSKNYRPEIASRIPPGQRLVKSWPVLHYGPIPTFDGSNWDL
                     ELSGLVENPVTLSYQELRGLPNVDVPADMHCVTGWTTLDNSWHGVSFRTLLDLAKPGP
                     EAKWVIAHCEYGYTSNLSLEAMTDDDVLVAWANGGRDLEPEHGWPLRLVVPKRYAWKS
                     AKWLTGLEFVDENKRGFWEVRGYHVHAEPFGEERYSYQEGPRAELEP"
     gene            complement(27920..28699)
                     /locus_tag="VF195_00650"
     CDS             complement(27920..28699)
                     /locus_tag="VF195_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6579376.1"
                     /translation="MPELPDVEHFRRTFARGAAGHRVSDVVVTDPGILRDVTAAELDQ
                     ALRGHRFEEPERRGKWVIAWTDGPALVMHFGMTGDILWAADAADRHAHDRVIVVLDHG
                     EIRYRNMRKFGGVWLAGNRDEVEGLLARLGPDALDLGRASFLRLLETRRGGIKAVLLD
                     QSFIAGVGNLLADEALWRARIHPARLIASLPIDDRITLFRQLRSVIHRQTVDSYPDGF
                     RSRWNDARQRGEGRCPRCRTELARTVVGGRTTYLCPSCQPR"
     gene            complement(28706..29089)
                     /locus_tag="VF195_00655"
     CDS             complement(28706..29089)
                     /locus_tag="VF195_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6579377.1"
                     /translation="MATLREIMTSDVFATTPSASVADVAASMLKGRFGSAVVMEGSTL
                     MGIFTERDVLRAAAARSDLTSSRVADWMTGDPVTASPDMDTGEATEIMMSQGFRHLPV
                     VEGTTIAGIVSFRDILRTRVRRTPS"
     gene            complement(29109..29357)
                     /locus_tag="VF195_00660"
     CDS             complement(29109..29357)
                     /locus_tag="VF195_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579378.1"
                     /translation="MDDASDRLLRIIEDYERVAEAVTPDDATRTFDETTLQLFWQRWP
                     QVSSWGGALWRQLNDDLADPATPAGDLETHEVGGEGGG"
     gene            complement(29382..30221)
                     /locus_tag="VF195_00665"
     CDS             complement(29382..30221)
                     /locus_tag="VF195_00665"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEX6579379.1"
                     /translation="MILGAHVRRRHTGIEGAIEEAALRGADCLQIFVSNPRAWAAPRY
                     EGPQIERFLDALTASGLRPCVAHLSYVGNVASWDPTTLRRTRELLVGTVHACDALGVD
                     LLVVHAGAGGPQDRETALGAAAESFRLAVGEADRTRVLVELMAGTSGAVASIPSEAEE
                     LCTAVDDERLGICLDTAHLFAAGVPLDSAEGVEGFAEELRVRALDRRLALVHANDSVF
                     ERGARRDRHADIGDGKIGEQGWRALVHDPVLGSVPWMLETPGDADRQRTDITYLRGLA
                     APV"
     gene            complement(30218..>31614)
                     /locus_tag="VF195_00670"
     CDS             complement(30218..>31614)
                     /locus_tag="VF195_00670"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEX6579380.1"
                     /translation="VKVADFGLARAYADGRVTQAGAVTGTVQYLAPEQIRGEPADPRS
                     DLYSLGIVAYELLTGKLPFTGETAMAVAYKHLSDRVPAPTSVLPDLPAELDGFVAAAT
                     DRDRELRPESADVMRMDLDVLSRQLPPARKLSTIVREIPSVTVEGGETTEVGVRLETS
                     IPRMERHRKRRRFVKRVLGAVALVALLTAAAWGTWTYAIPHTAVIPALVGEAVDDATD
                     RLTGLGFDVTRAPGEYSMQVPDGHVKQILPAAGAERDVGTTVTIVPSLGPPPVDVPRV
                     RGEPLQDAREGIAGAGFHVGAVRHRFDREVPEGHVIGVQPDAPRLPRGTSVTVIVSDG
                     PKPVAIPDVGDMTGDKAVKALTTKGFEVVIEEDFSNKVARGRTLGTDPESGTELQPGE
                     TIVLRVSLGPEFFACPDFEGMTVDAARALAEDVGLELTALPVPGSSGGSIVSQLPPAG
                     TRVRYGSTITVYYA"
BASE COUNT         4958 a        10746 c        11218 g         4692 t
ORIGIN      
        1 aggatcgggg atcgcgtccc gctcgtgacg acgccgactt gctggcggcc ggcgtagacg
       61 ccgtcgccgt gcgcggccgg ctcgttgccg tcgagctcga gtccgacgag cgcccgttgt
      121 ggatgcgcgc gccgctccgc gagcgcctcg cccccgacga agtcctcgtc cttgccctcg
      181 gtcaccgtga aaccgatgcc cgcctcgaac ggatcgacct gatcgtcgaa ctcggaaccg
      241 gcgaagatca ggccggcctc gatccggagg atgtccagcg cgtcgaggcc gaggggggcg
      301 agaccgtgtt tctcgccggc cccccagact gcatcccaca ccgtgagcgc gtccttcgga
      361 tggcagaaga tctcgtagcc gagctcgccg gtgtagcccg tacgagagat caccgccggc
      421 acgcctgttg gtccgccgag ccgtcccacc gagaatcgga accatcccag gccctcgaac
      481 ggcgtctgag caggtgggac ccacacgatc tccttcagca gctcacggct ggctgggccc
      541 tgaaccgcca cgttgtgcag gtgatcggtc gactccttga cgaacgcgtg caggccgaga
      601 cgcgaggcct gctcgcggag ccagatgccg tcgtactcgg ccccgcccac gaaccggaag
      661 ttcgtgtccg cgatccggaa gatcgtcgcg tcgtcgatca tgccaccgtc ctcgttgcac
      721 atcgcggtgt agacgacgtg gccgtccgag agccgccgga cgtcgcgcgt cacggtcgcc
      781 tgtagcaacg cctcggcgtc gggtccgagc acctcgaact tccggagcgg cgagagatcc
      841 atgatcgcgg cccgttcccg gcacgcccag tactcgtccg tcgcgccgtg gttatcgaag
      901 ctcgccggca gccagtaccc gttgtactcc gtggtccggc tcgtgagctc agagatccgg
      961 ggatggaagc cggtctccgt cgtgagggtc ggatcggcgt cgggcgtcac ccggtgcgcg
     1021 atcgcggctg agaacgtgtg ctcggcggag tagacacgga cgtgcacctc ggtcgggtcc
     1081 catccgttcg cggggtcgac atcgtcgggg catgcgctcg aggcgcagac gaggtccgtg
     1141 gcggcgcgca acagtacgta gtcgcccggc cgggaccacg gctcgtcgaa gacgaggagg
     1201 ttatcggtcg cgaacatcgt gttgtagaag aagttgagcg cttgccaccc cttccgcggc
     1261 gcgatcgcgt acgtctcgag ttgcctgttg aagttgtcgg tgcagttcgt gtgcccgaag
     1321 tacccgaggt tctcgtagta ccgggacgtg caggccagca ggaacgagtc gtgtcgtccc
     1381 accgtgtccc ggatcacctc cacgaggacg tccatgtcga ggtcgtagaa cttcgagtac
     1441 agcccgggct gggggaacgc gttgcccatc agcgtccgcg tgatcgtcgg gtcgaagccg
     1501 cgctcggtcc cgccctcgag cttccctcga tggaacgcga ggaagtccga gcactgcttc
     1561 ccctcgacgt cgatgatctg gatgtactcg ccggccttga cctcgtagga cagagccgtc
     1621 gcccggtcga cccggaaatc caggcggggt tccgcgagcg ggggaggcag ctccagctcg
     1681 acgtgggtcc gcgggcgcgt ccgccggatc tcgacatcga ggtccgaccc cgggacgccg
     1741 ccctccagca cggacacgcc cgcgggcgcc gcgaccacca cgaccacgga acgctcggcg
     1801 gtgaacgtct gggaagagcc ggcgggggac cactcaccga acaaccggac cgccgtcgcg
     1861 tccgaggggt tgaggccccg cccggcgagc gatccgatca cgtcggaggc gccctcggcc
     1921 tccgagccga tcagcgaccg caggaccgac gcggggacgt cggcccgggc gccgagcgcg
     1981 gcggcgtcgt cggagccgtc gggtgccagc gcggtgatct ccgcgacctg accgccgtcc
     2041 ggatcgcgaa ccgtgagctg gtcgtcgccg tcgagggcca ccacggcggc tccaccagat
     2101 cgcacccagt accgctcgag gaacggatcc gcggcgatca gccccgggac ggtgatgctc
     2161 cggcgtccgg tcgagatcgc catcggcatc agcccttggc cgcgggctcc ttcgacgtct
     2221 gctcgtcggc ctccttgaag agctgctcgg cgagctctcg cttcccggcg gcgtcgagct
     2281 tcttgacctc gttctccaca gcccgtcggt tgtaggactg gttccaatag tcgagcgact
     2341 cgaacccgag gagcaccgcc ttgccgacga actgccgaag cacctcgttg gtcgggccca
     2401 tcacccagcc ggccttcgcg tccctgaggt accgctcgag ctccatgtcg cgcttgtagc
     2461 cggatccgcc gcaggcgtgc agcatcttgt cgacgacgtg cgcggtgttc tttgccgcct
     2521 cgaacttgat ctgccaggcc cagtggagga agttcccgcg cgccaactcg cccggtcccg
     2581 ggttcacggt gttgttctcg gtggcgccgt ccatcgcctg ggcgacggag agcgtgaata
     2641 gccgcgaggc gttcgtgtcc atcacggcct cgccgacgta atcctggatc gtcgggtagt
     2701 ccgcaacgcg catgccgacg tcgacgtgct tcttgcgcgt ggtgtggcgc ttcgcgatgt
     2761 cgatcgcccc gagggcgagt ccgttccaca ccgaggcaga ccccaccagg aaccaggggt
     2821 ccaccgactc gtcgttcgag ttcgcgccgt cgccggtggg gcctacgagc tgctccgcgg
     2881 ggatctcgac gttgtcgatc tcgatcgggc cggactggtt gccccgcaac ccgagcgcgt
     2941 cccacaagga cggctgcgcc tggatgtgct cgccgtcgac gacgaacacc gagaggtcgt
     3001 cgtagccgga gaagtccggg ctggtggtct gcaccacgta gaagtcggcg aaccctcccg
     3061 acgtggtcca cgatgccttc ttccggacct tgaagccgcc gttcgagcgc tcggcgcccg
     3121 aggagatcgg gtaccagaag tgcgatccgg tctcggggtc ggagtaggag agcgtgccga
     3181 tcttgccgga gttcagcggc cggatgtact tgtcgatcag ctcgggggtg gcgcggagca
     3241 tgatcgcgtt cacggcgcct atgtgcatca cgtagcacat cgcggtcgag gcgcagccgt
     3301 agcgcgcgat cgtctcgcag accatcgagt aggcgacgtg gttctcgccg aggccgccgt
     3361 attcctccgg cacggtcagc gccaggaagc cgtgctcgcc gagcagctcc aggttgcgac
     3421 gggggaacat gagctcgtcg tcgaaccgct tggcgttggc gcggagctcc ttctcgcaga
     3481 gctcgatcag ggtggcgcgg agctccttct gcttgtccgt gtagacccag tcggggtccc
     3541 agtcgtaccc gagtccccag aacggctcgc caccccacat cttgtctgtc atctcgacct
     3601 cctgacggat ccgcttcatg ctaggcgctg cctcgtgggg cgggcaacgc tcggccccgg
     3661 ggacgaagaa aggggcggcc ctccgccccc tcctccggtc gttcctggtc cctaggagga
     3721 cggttccggc atgacctcct cgcggagcaa ccgcatctga ttctcgctcg gcatcgtggg
     3781 cacgtccggg tctcggaacg tgatcggctt gcggtcctgg acggcccgcc ggtagaagaa
     3841 cagcgccacg gacgcgacga gcacgccggc ccccaggaag agtgggaggc ccttgaactc
     3901 gtagaagccg atgtacgacg cgaccttctg atcgatcaca ccggcgagca cgaggacgag
     3961 gttgaaggcc gccagcggcc aggcgagcgc cgtgaacacc ggcccggcct tgatcggtct
     4021 cggccagtcc ggacggtccc tccgcaagag caggaacccc gtgagtgcga gcacgtgaca
     4081 gaacacgtag ccgatgttcg agatgtagag gatcacgaag ttgttcgcgc ccagcaacag
     4141 caacaggccg atgttcacca ccatgtcgac cgtcatccct cgcgccggga cgtggaactt
     4201 gttcaggtgg tacagccatc tgatcgtcat gtcgtcgcgg gcgatgccgt agagcgcgcg
     4261 gcctccgtcg gcggtggacg tgttcatcgc gaggatcagg ctcgcgatca ggcagatcac
     4321 ggcgaacccc cccaacgcgg agccggcgat ctcctccaga gccgccacgt agaaactgcc
     4381 gtagtcgccg accgatccca ccacgccccc gaggccgagc ggcaggaaga ggtagacgaa
     4441 caacgagaac acggccacgc tcttcagcgc tttcgacgtg tcccgcttcg tgtcgtggta
     4501 ctcgggcgcg aagctcgcgc ccacctcgat cccatatgcg gaccagcaca tgatgaagag
     4561 ccacatcagg gcgatccgga ggtcgctgaa gccgccggcg ttgtggacgt actccaggtt
     4621 ggaggagctc cagtcccccg tgaggtaggg gaagatgatg aagatcgcga ggggaaccac
     4681 cagcagcgcg ccggtgacgt acccgaccgc cagcgagggc ttgaccccca ggacgttgaa
     4741 gagccagacc gcgaggatca cgatgatcgc gatcgcctgt gccagcccga agtccgcgaa
     4801 cccgaggttg aagtaggcgt cgtccacccc gtcgcccgac ttcgggaacc attctgcgac
     4861 gatgagggct cccacgatgt tcccgacgaa cgagagcacg acggaccagc cgatccaata
     4921 tccgaacgcc gcgatcggac cgaagaggtt gaaacgagac cgccagccct cgtgcgcgta
     4981 gagggcgatc cctccgggct tgtcggggaa catcgcggcg gtctctgcgt agatgaagtt
     5041 ctgcagcaac ccgacggtcg cggagatccc ccagaggagg atcgatcccc acgccccgat
     5101 cgtggcgagc gtgaagccga gcgacccgag taggaacgcc ggctggcata agccgatgac
     5161 gaatccgtcg taccagttga gcgacttgag gagatggcgt tcctcgatgg tcgcctgcgc
     5221 cgtgacctcg tcagccacct gatccctccc ctctgtgtgc gcggccctcc ggccgcatgc
     5281 cgcggtatag cacggcactc cgagccgggc aatccggggt tgttgcctcc gaccgcatcc
     5341 gagtcccacc tgtaccatcg ccgccgtgaa aggccctccg atcacggtgc gatgcgattg
     5401 cggcgaggtc gaatcggttc cctacgggga ggcgtggacg tgccccacgt gcgggcgccg
     5461 ctgggacacg gcgcagatcc cgtccgacga gtactggagg gtcatgcggg agatgcggcg
     5521 gtaccggctc gaggtgatcg ccgtgtcggt ggtgctcggg atcgcgctcg cgatcacgct
     5581 cgcgaacctg gggagcagac ggtacttccc gatcgtcctc ggcgtgatgg gcttctggtt
     5641 cctgatctac atgccacgct ggagacagaa ggtgcggcga cgcgttcgag agctccccgg
     5701 ctggcgattg cggtcgggct gaggctcgtc cgatagtctc agccgcgtcc tcggcgctcg
     5761 gacgaggcca tggccggagg agcatcggag gagcggggtg accttcctga tcacgtgtcc
     5821 gaactgcggg ccgcgcgagg ccctcgagtt ctccttcggc ggggaagcca cacgtcgagc
     5881 aggacccacg gctaccgatc ggcagctggc ggccgctctc tatttccgcc gcaacgtcga
     5941 cgggtggcag acggagtggt gggttcatca gagcggctgc cgcgcatggt tcctcgcgga
     6001 acggcatacg acgacgaacg agatccggcg gacgtaccgg ccgcaggagc gcccgcgggg
     6061 cgaggaggac agggaggccg cgcccgcgtg agcgagcgtc tcccggcgcg tcagggcgaa
     6121 gcgatccggc gcgaccggcc gatcaccttc cggttcgagc gcaggacgat cgccgcgttc
     6181 gagggagaca ccgtgggatc ggcgctgggg gcggcgggcg tcacgatcac gggtcggtcc
     6241 ttcaagtacc accggcctcg cgggctctcc tgcatgaccg gctcgtgccc gaactgcctg
     6301 atgcagatcg acgggatccc caacgtgcgc gcctgcacgg agccggcgcg ggacgggatg
     6361 aaggtcgaac gtcagaacgc gtggccatcc gccgaccggg acatccacgg atggctgaac
     6421 acgttctcgt tcatgatgcc gccgggcttc tactacaaga tcttccagcg ccctcgctgg
     6481 gcatggccgc tcgtggaacc gttcatccgc tcgaaggccg ggctggggaa ggtgccccac
     6541 gagccagatc accggcggcg ccggcggatc aacctccacc ccgacgtcct cgtgatcggg
     6601 gcgggggccg ccggcctcgc ggccacggcc gaggccgcga aggccggtgc atcggtcgtc
     6661 ctgctcgagc agggccgcga ggccggcgga cacctcttgg gagaagcgag cagcaccctc
     6721 cgatcggggc tcgcgcggga ggccctcggg tccggagccc ggttcctgcc ggagacggcg
     6781 gcgttcgggg tcttcggtgg cgggctgatc gcggcggccg gcccggacgc cctgtaccgg
     6841 atccgcgccc gacacgtgat cttcgcgacg ggggcggtcg agggatcagc ggtgttcccc
     6901 ggtaacgacc tccccggcgt gatgctctcg tccgccgttc atctgctgct ccaccgctac
     6961 ggcgtgctcc ccggccgaag ggccgtggtc ctcaccggcg acaacgtcgg ctacgcgacc
     7021 gcctgggccc tgaaggatgc cggggcggag gtgacggtgg tcgatctccg ttccgacggc
     7081 ggctggccgg aggggttccc cgtgttcgcc gggtcgacga tcctgtccgc gcacggacgg
     7141 cggcgggtga gcgccgtgtc cgtcggcacc gtcggagcga cgtccggaca gacggtctcc
     7201 tgcgatctcg tcgtgaacgc gtgcgtgcag acgccgtcca cgaacctcct cgcgcaggcc
     7261 ggcgcgcggc tggcattcga tgcgacgttg caggccttcc tcccggccga gataccagcc
     7321 cgcgcatggg ccgtcggggc ggttgcggga gcacgctcgc cggaggcctc cgtggcgcaa
     7381 ggaaggctcg cgggcctgga ggccgcggcg ggctcgggtc atcaggtgga cacggcgtcg
     7441 atggcggcgc tccggagcga ggccgtccgt tccggggacc cgatcgtcct gccgccggag
     7501 gcctccgccg cgtccgggaa acagttcgcc tgcatctgta tggacgtgac cgcgaaggag
     7561 ctcgagacgg cggtggccga gggcttcgac tcgatggagc tcctcaagcg ctacacgacg
     7621 atcacgatgg gtccctgtca ggggaaggca tgcatggcct cgtcgcaacg gctctgcggc
     7681 gcggtcacgg gcaggtcctt cgccgatacc tcgcccacca cgtcgcggcc gccatgggta
     7741 ccggtggagc tgggcacgct ggccggcgcc cgcctcaccc cgcggaagga gacgacgatg
     7801 cacgaccgac acgtcgccgc gggggcggag ttcatgtggg ccggggactg gcgacgtccc
     7861 catcactaca cggggccgga agcggaggtc gccgccgttc gacaccgggt cggtctgatc
     7921 gacgtgagca ccctcggcac gttcaggatc agaggccccg aggcggtcga gctgctcgaa
     7981 cgcctctacc cgaaccggta ctcggacctc gccgtcggtc gggtccggta cggcgcgatg
     8041 ctcaacgacg agggggtgat cctcgacgac ggagcggtcg tccgcctggc cgacgatgag
     8101 ttcttcgtca cggtgacgac ggggaacacg gccgcgctgg aacgatggat cacctggtgg
     8161 aacgcggact ggggtctcga cgcgctcgtc ctgaacgtga cgggggcgta cgcggccgtg
     8221 aacctggcgg ggccacgcgc ccgcgccgtg atggcctcgc tcaccgacac ggatgtctcg
     8281 ccggcggcga tgccgtacat gaccgcgacc aggatggcgg tggcggacgt gccctcgctg
     8341 gtgctgcgga tcggcttcgt gggggagacg ggatacgaga tccacttccc atccacctac
     8401 ggcgaccatc tgtgggacgc cgtgatggaa gccggccgct ccgaggggat cgtcgccttc
     8461 ggcctggagg cccagcggat cctccgcctg gagaagcagc acatcctcgt gggacaggac
     8521 acggatgccg aatccgaccc gtacgaggct gggctcggct ggatggtgaa ggacgacaag
     8581 gaggacttcc tcggtctgcg gtcgctccgg gacctgaagg accggggccc tcgcgagcgg
     8641 ctcgtcgggt tcacgtcatc ggagcgctgg ctgccacccg agggcgcgtc cgtggtccac
     8701 gagggagcgt gggtcggtcg ggtcacctcg gcgaggcgca gcgaggccgt cggtggcgtg
     8761 gtcgggctgg catgggtgcc ggcggactgg gccgcggacg gccgcacgtt cgagatccag
     8821 ttcggcggga gccacgcgac cggcacggtc cggaccgatc cgttctacga cccgcgcggc
     8881 gagaggctcc ggtcatgagc acggcgatcc gacgctcgcc cgcggcgcga ctgtcgtcgc
     8941 gccgcggagc ggtgttcgtg gaggaggcgg gttgggagat cccggcctcc tacgggaacg
     9001 acgcggggga gcgcgcggcg atccgcgatc gcgtggcgat cgtggacatc accgcgcgcg
     9061 ccaaggtgga catccgcgga cgcatcccgg agacgctgcc ctcgacggcc gacaccgtcc
     9121 tcgcgcggat ctcggcggag tgggcggtgc tgctcggagc cccggacgcg gaaggaaggc
     9181 tgatccgggc gatcgaaccc gtcgccggtg caggcgccat ggtgaccgac gtcactcacc
     9241 tgttcgccgg cgtggcgctg gctgggccgg cgttgccgga gctgatcgaa cgtgccacgt
     9301 cgtgggatct ctcgaccctc ccggcgggtg cggcgaccgg cgcaccgatc gccgaggtcc
     9361 gggcgctgct ggtccgccgc ggcctcccgt tccccgtcct cgaggtgttc gtggccatgg
     9421 agttcggccg gtacgtatgg gagaccctcg cgagcatcgt gagcagcctc ggcggcgcgc
     9481 cggccgggtg gcaggccctt cgagcggaag gctggcgctg atgcacccga tccgctactt
     9541 ccggccgtgg cgcatgtggc gccgacacga gcggaaggat gcctacgacg tcgtgatcgt
     9601 cggcgccggc gcccacgggc tcgcgatcgc ctacgagctg gcgaagcgcg gcatccgcga
     9661 cgtcgccgtc ctcgacaaga gctacatcgg cgccggcggg tccggtcgca acacgacgat
     9721 cgtccgcgcc aactaccgga cgcctgaggg cgtggccttc tacaaggagt cgctccggct
     9781 ctacgagcag ctcgcgcagg agctggacta caacctcctg ctctcccaac agggccatct
     9841 gacgctcgcg catgcagagc gcgcggtgat cgtcgcgcac gagcgcgcgg aggtgaaccg
     9901 gctcctcggt gtggactcgc gcgtgatcta cccggacgag atcgcgaagc tctgtccgga
     9961 gctcgatctc tccacccacc ccgacttccc gatcatggcc gcgctctacc acccgccggg
    10021 gggagtgatc cgacacgacg cggtggtgtg ggcgtacgcg cggatggccg atcggatggg
    10081 cgtccatatc caccaggaca ccgaggtgac tggcatcgac gttcgccacg gtcgcgtcac
    10141 cggcgtccgg acgaaccggg gcgacatcgc cgcaggcacc gtcatctcgg cggtggcggg
    10201 atggacgagc gaggtctgcc gcatggcggg ggtggagacg ccgatcgtca cgcacccgct
    10261 gcaggcgttc gtgacggagc cgctgaagcc cttcctcgac gtgatcctgg tctcggccac
    10321 gctgcacgtc tacatctcgc agacggaccg gggcgaggtg ctgatcgggt cggagatcga
    10381 gccgtacacg tcctactcac aggagtcgac gctcgacttc ctcgaggtca cggcggcgca
    10441 ctccctcgag ctcctcccga tgctcgggag ggtgaagctc atgcgggcct ggggagggct
    10501 ctgcgacgtg acgcccgact actcgccgat catgggcctg acggacgtgg aggggttcct
    10561 gatcgacggc ggctggggga cctacggttt caaggcgacg ccgatcgtcg gcgttacgat
    10621 ggccgacctc atcgccgaca accgggtccc cgatctgatc gcgccgttcg ctctggagcg
    10681 gttctggaag cgggagctcg tctccgagat cgccgccgcc gccgtttccc actgacgcgt
    10741 ctcgatcacg gacacctggg gacctccggc atcgtaggtg gggaagtgga ccccggcggc
    10801 gccaccggat cgctcgaaca cgcggcgagc aggatcacgg agccgaggag ccatgcgctg
    10861 gtcacccgtt cggtcccgcc gggaaggacc ataatcaaca tgatggcgac cgccgcccag
    10921 ccgctccgcc ggaacacctc gagcgtgcac gtcgtggcga tgaacgacgg ggtgcggtcc
    10981 gagcgaaccg acacgctcgc cgccgaggag ccgctcgaga tccgcgtgca ggggcccggt
    11041 caggacctga gatctgtggc cgtcacgatg cgaacacccg gtggcgactt cgagctcgcg
    11101 gtggggttcc tgttcacgga gggcttgatc gcacccggcg acgtgagccg tgtggcgtat
    11161 tgcgacaacc tgcccgggga ggatcagcag tacaacgtcg tgtcggtcac gctcgatcgg
    11221 ccgttcgact tcgacctgct gaagcggaac ttctacgcca cgtcgtcctg tggggtgtgc
    11281 ggaaaggctg ccctcgacga catcgtcgtg cggtgcgccc cggtcgcgac gggcacgatg
    11341 gtgacgatcg agacactgct cggcctcccg gatgccctcc ggaaggttca gaaggtcttc
    11401 gaccgcaccg gcgggctcca cgctgctgga ctcttcacct ccgagggaca acccatctcg
    11461 gtccgcgagg acgtcgggcg tcacaacgcc gtcgacaagg ccgtggggga gcagctcctc
    11521 gccggaggta tgccgctggc ggaccacgtg ctccaggtga gcgggcggct ctcgttcgag
    11581 atcgtccaga aggcggccat ggccggcatc ccgatcgtga gcgcggtgag cgcgcctaca
    11641 tccctcgcgg tcgaggccgg cgagcggttc gggatgacgc tggtcgggtt cgtccgcgac
    11701 gagcggctga acgtctacac ccacccggaa cgcgtcggag gatcagaacg tggagacgag
    11761 gcctaggaga cggcgacgcc gacgaggttg ccgaccccgt acgtgatggc cgctgccacc
    11821 gcaccgatcc ccagcatcct tgcccccgag accagcacgc cgcgaccggt caggacggtc
    11881 atcgcagccc cgacggcgaa gagcgtcact gcggacaggg atgcagagac ggcgatcgcc
    11941 gtcttgccag aggtgaacag gaatgggagc aggggcgcga ccgcgccgat cgagaacatc
    12001 acgaacgagg agcccgcggc cgcccagggt gatccgagcc cttccttgat gtcgatcccg
    12061 agctcttccc gcgcgtgggt ctcgagggcg acctccggat cttccatgac ctctgccgcg
    12121 atccgcgctg cggtctcctt ggagatcccc ttccgctcgt agatctccgc aagctcgcgg
    12181 agttcctcct ccggctgggt ggcgatctcg tgggcctcct tgtggatcag ctgttcgaag
    12241 acctcccgct gcaccatcat cgagatgtac tcgccggatc ccatcgagaa cgcgcccgcg
    12301 agcagacccg cgaacccggc gatcacgacg acgtcggcgg ccacgccgga gcccgcgacg
    12361 ccgaagatca gcgagaggtt ggacacgagg ccatcgttca tcccgaagat ggcggcccgg
    12421 agggcgcctg acttgctgct ggaggcgtcc tgctccgccc cgacgggacc ggggtgcggc
    12481 gtgatccgcg agccggtgct gccccggaga cggtcgagca tgcccacccg tggttgcctc
    12541 ggcgcccgtt cggtcatccc tcccacacgt tagacggtgg agcgcgggcc gaccccatcc
    12601 cggccgcgat agggcgctta gaatccccgg gcggccggga cgtccggtcg aggatcaccg
    12661 agccaaggaa cgcgggaagg agcgggatgc cgaacgggct cacccgactc accgagccga
    12721 tggtgcgcga agacggcgag ctgcgcgtcg cgacctggga ggaggcactc gaccgggccg
    12781 ccgacggttt gcgggccgcc accgagcgcc acggcggcac gggggtcggc ctgttcagct
    12841 gctcgaaggc cacgaacgag atgaacttcg cagcgcagaa gttcttccga caggccctcg
    12901 gcagcaacaa catcgacagc tgtaaccgca cctgacacgc gccgagcgtg gtcggtctgg
    12961 ccacggtctt cggcgccgga ggcggcacca gctcatacga cgaggtcgag cacaccgatc
    13021 tcatgctgct ctggggctcg aacgcccggg cggcccaccc gatctacttc caccatgtcc
    13081 tgaaggggat ccggaaaggc atgcgactga tcgtcgtcga cccccgccgc accgagtccg
    13141 ccgagtgggg ggacctatgg ctcgggctgg acgtcgggac cgacatcgcg ctctcgaaca
    13201 cgatggcccg ggagatcatc gtgaaccacc tgcatcaccg cgcgttcatc gagctcgcga
    13261 cgacggactt cgaggactac gcggcctcgg tgatggactg gacgctcgag cggggcgcgg
    13321 aggtcacggg gatcccggcg caggtgatcc gcgaggtggc gctcgccttc gccaaggcgg
    13381 accgcgccat gatctgctgg accctcggga tcacggagca tcacaacgcc gccgacaacg
    13441 tgctggcgct gatcaacctc ggcctgctca ccggtcacgt cgggacgtac ggatcggggc
    13501 tgaacccgct gcgcggccag aacaacgtcc agggcggcgg cgacatgggc gcgatccctc
    13561 accacctgac cgggttccag gaggtcgaga acgatgccgt gcggacccgg ttcgagcagg
    13621 agtggggggc caagatcaac ccgaagaagg gattgcatct gtcccagatg ttcgaggcta
    13681 tggaggaggg cgagctcacg gcgctctatg tgatcggaga gaacccggcg gcctccgagg
    13741 ccgacaacca gcgcacgcac aagctgctct ccaacctcga catcctgatc gttcaggaca
    13801 tcttcctgac gcagaccgcg aagatggccg acgtggtgct gcccgccgcg aactcggcgt
    13861 tcgagtccga gggaaccgtc acgaacagcg agcgccgcgt gcagcgggtc cggcccgccc
    13921 tcgtgcctcc cgggaacgcg aaggacgaca tctggatcat ctcgcagctg gccgaccggc
    13981 tcggccggga ctggggcgat ccctccgcgc acgacatgtg ggaagaggtc cggcgcctct
    14041 cgccgatgca cgccgggatg gcgtgggagc ggctcgagga gctcggcggg attcagtggc
    14101 cgtgcccgga cgaggagcac cccggaacga cgttcctcca cgggcggctg tggtcggagg
    14161 atcctgaagc gcaggggagg aaggcgccgt tctccgtggt gctctatgag ccccccgtgg
    14221 acgagctcga tgaggactat cccttccggc tgaccacggg ccgtcggctg gactcctaca
    14281 acaccggagt gcagaccggc gagtacacgt cgccgctccg tcgcgaggag acgctggaca
    14341 tctccccgga ggacgcggag cgcctcgggt tccgcgaggg ggagcgggtc cgggttctgt
    14401 cccgacgtgg cgaggtcgtg gccccggtcc ggtacgcgct gggactgcga cccggcctcc
    14461 tgttcatgac gctgcacttc ccggaccagg tggacgtgaa caacctcacg atcgacgcat
    14521 gggatccgaa gtcgggaacg gcggagttca aggcgaccgc ggtccgcgtg gagaagcttc
    14581 ccgcggcgat cgcctcaccc ggcggatgag gggagccgcg tggatctcca tctgatggac
    14641 gcggctccct cggaggccga gcgtgccgcg gtcgacgggc tgctcggacc gccggagacc
    14701 ggctgggtgg gcgccacgga gcggagcgcc gctgatctcc gcgtcgtccg cgccggtcgt
    14761 caggcgcgtg agcagcggca cctcctgctg ccggccttgc acgcgctcca ggcgggtgtc
    14821 ggctggatca gccgcggggg gctgaactac gtctgccagc ggctctccgt cccgccggcc
    14881 gatgcctacg gggtggcgac gttctacgcg atgttcagcg tcgaggagcg acccgccaac
    14941 gtggtccacg tgtgcgacga cctcgcgtgc cgtggagcag gaggcctcgc cctctgcgga
    15001 gagctcgagc gcgacgcggg accggaaggc gtcgatgcgg gcggctggac gtgggtccga
    15061 tcgccgtgcc tcggcatgtg cgagcaggcc cccgccctct tcgcgcagcg cgtgggccgg
    15121 ccggaggtcg ccctcggagg ggcgacacgc gcgaccgttg acggcatcgt ctcgccggac
    15181 tccgcggtgg tgtgcgccgg cggggtggga gccccgcaga cggcggatgc gagatcgcgc
    15241 gacagccttc gactcctgcg gcgcgccggc gtcgtcgacc ccacgtccat cgacgactat
    15301 cgcgcgcacg agggatacgg ggcgctccgc gcggcgcacg agatgggtcg gggcagcgtg
    15361 atcgaggccg tgacggaggc gaagctcctc ggtcgcggcg gcgccgcctt cccgaccggc
    15421 gtcaagtggc aggccgtgtc cacccagccc gcggggccgc gctacgtggt ctgcaacgcc
    15481 gacgagtcgg agccgggcac gttcaaggac cgcgtggtga tggagcagga cccgttcgcc
    15541 ctcgtcgaag cgctcacgat cgccgggttc gcgtgcggtg ccgagaaggg tttcatctac
    15601 gtccgtggtg agtacccgct cgcgaccgag cgcctcgagc acgcgatcgg ggaagcccgg
    15661 cgccacggcg ccctcggcca tgacgtgatg ggcagcgaga ccgcgttcga catcgagctg
    15721 cgccggggcg ccggcgccta catctgcggc gaggagacgg ccttgttcaa ctcgctggaa
    15781 ggcaagcgcg gtgaaccgcg gaacaagcca cccttcccgg tcacgttcgg cctgttcggc
    15841 aggccgacgg cgatcaacaa cgtcgagacc ctgatcaacg tcctggaggt cctccgcatc
    15901 ggggccgacg cctacgctgc gatcggcacc gaggggtcca ccggaccgag gttgttctgc
    15961 ctctccgggg ccgtcgagcg acccggggtc tacgaggtcg agcacggcgc gaccgtcggc
    16021 gacctgctcg cgctggcggg cggtgtccgt caggggagcg cgctgaaagc ggtcctgctg
    16081 ggtggagcgg ccggcggatt cgttggcccg gacatgctcg acgcgaggct gacgttcgag
    16141 gacgcgcggc agggcggata cacgctcggc tccggcgtga tcatggtctt cgacgagcgt
    16201 gcggacctgg tggatctgtg cctgcggatc gcgcggttct tccgtgacga gagctgtggg
    16261 caatgcgttc cgtgccgcgt gggaaccgta cggcaggagg aagcgctcca tcgactcgtg
    16321 gcggggaccc cgatcggctc caccggggac gagctcgccc tgatcgacga ggtggcccgc
    16381 gtgatgcgcg acgcgtcgat ctgcggcctc ggacagacgg cggccaacgc cgtccagtcc
    16441 gcgatctcca agctgagcgt cttcgacggg ggtgccgcgt gagtcgggcc gtgccgctcg
    16501 acccgccccg acgactcctc gacctggaga tcgacggacg aacgacgcgg gtccccgagg
    16561 gcgcgacgat cctggacgcc tgccgggcgg agggcgtgga cacacccacg atgtgctacg
    16621 cgggcaacct cacgccggtc aacgcctgcc gggtctgcat ggtggaggtg gagggctcgc
    16681 gcgtcctggt cccggcgtgt tcccggcccg tcgaggacgg gatgaaggtg cagaccgaca
    16741 ccgaacgcgt ccgtcattcg cggaagctcg tgatggagtt cctcgcttcg agcgtggaca
    16801 cgagcctcgc ctccaccgat tggctccgat ggatggggga gtacggagcg gatccgtcgc
    16861 gctacggggc cccgatggat cctatggagg gcggcgagcg cgacgagcgg tgggccggtc
    16921 accatcacga ccccgacccg gcgatcgcgg agaccgtcgc ccagcccgtg aaggtggaca
    16981 acgagttgta cgtgcgcgac tacgagcggt gcgtcctctg ctacaagtgc gtcgaggcgt
    17041 gcggggtcga cgcgcagaac acgttcgcga tcgcggttgc cggacgcggc ttcgacgcga
    17101 ggatctccac ggagttcgcg gtgccgctgg acgaatccgc ctgcgtgtac tgcgggaact
    17161 gcatcggcgt ctgtccgacc ggtgccctga tgttcaagtc cgagcacgac atgcgggcgg
    17221 aggagacgtg ggacgaggaa gcgcagaccg tcacgaacac gatctgcccg tactgcggcg
    17281 tgggatgcga gctggaggta cacgtgcagg acaatgagat cgtcaaggtg acgtcgcccg
    17341 aggatcactc cgtgacgagc ggccacctct gcatcaaggg ccggttcggg ttcgcctacg
    17401 tgcagcggcg cggtgaccga cgggggagcg accggggtgg cggggcgccg atcccctcac
    17461 cgacggccgg gggagaaggg gcgacgcccg agaaccctgg ttcatgaggt cgacgggcgg
    17521 gcccggggag ccggaggccc cgatcggcgg gatcgtgctc gcgggtgggc gatcccgacg
    17581 gttcggccgc gacaagctcg ccgagcagat cgatggtcgc ccgttgctcc accatccgat
    17641 cctccgactc ctcgacgtct gcgatcgcgt cgtcgtggtg atcggcgcag atgtggagga
    17701 accgcccatg cccgtcggtg cctccgtcac gttcgcgcgc gactcgaccg ccgacgaggg
    17761 cccgctccgg gggctggctg cggggctcga gatcgcccac gcgacgtggg cggtcgtagc
    17821 gggcggcgac atgccggacc tccaaccccc ggtgctccgc gagatgctgc gggccgcgcg
    17881 agagaccgcc gccgtcgcgg tcgtcctctc cgacggcggg gaagccaggc cgctgccctg
    17941 cgtcgtgagg accgagccgg ccgcggatgc cgtcgccgtc ctcctcggga gtggaaggcg
    18001 gaggctcggg gatctcctct cggccgtcag gaccgtcgtg gtggacgagc cgtcgtgggc
    18061 cgccctggat ccggggcgtc ggaccctcgt ggacatcgac gagccggccg acgtcgagcg
    18121 cgcgcctcgc cgctaggctc gtgcggtgcc gcacgagccg ttcatgctcg gatcggtcga
    18181 ggtcgtgccc ctctgcgacg gatgggcacc gttgccgctc gacgacgagg cgcccggtcg
    18241 cgacgtcgac tgggatgaac aacgaacccg gttcccgtgg gcgttccctc ccgacgacgc
    18301 gacgagctgg gcgtggcacg tccactcctt cctgatccgc catccggacg gaccggtgct
    18361 cgtcgatacg gggatcggcg gcctcggccg accgcccttc gacgtcgcgg ggcggctcga
    18421 ggacgagctc gccacgatcg aggtctctcc ggagagcgtg cggcacgtga tgctcacgca
    18481 tctgcacggc gaccatgcgg gcggtgcgtg cgcggccgac ggcggcccgc tcttcccgaa
    18541 cgctcggtac cacgtgcatc cggacgactg gacgttcttc gcaacccacc gcacgccgga
    18601 ggatctcacg agccgtttcg cgatggccgg cctcgagggg ctcggcatgc tcgacctcga
    18661 cccgacggat cacgaggtca tggcggggct ctccgtacgg cacgcgccgg gtcatacgcc
    18721 gggccatcgc gtgatccagc tcgaggatgg gacgagaacc ctgctgctcg tcggcgacct
    18781 cctccacacg acgccgcagg tggcgcatcc cacgtggctg tcgaaccacg acgaggatcc
    18841 cgagctcgcg gcagagcatc gtgcgctctg gatcgacgcc gcggacaggg agggatggac
    18901 ggtctgcgtc gggcacttcg gccggccctt cgggcacgtg gcggcggccg gctggtcccc
    18961 ggtctgatct gccgcggcta cggttcccgg ctcgcctcca gctcgtcgag ccgttcggtc
    19021 gccagtccgt gggccgcctc tgctgctgcg tcgtgaccgg tgatggcccg gaggaactcg
    19081 tcccgatcga gtgccaggag cttgctctcg gtcgtggcga ccacggtcgc ggtccggggg
    19141 acgtcgcgga tcagggcgat ctccccgaac gatccgcccg gccccagcgt cgtgacccgc
    19201 cgaccacgct cgaggacctc cacgccacct tcgatgatcg cgtagaagcg atcgccggcc
    19261 tcgccctgcg tgatcacgac ggatccatcc ggcacgtgga tcgggatgag gttcctcgcg
    19321 agccgctcga gcatcggcgt ggggagcgga gcgaagaacc gcgtcgagcg aaggagcgcc
    19381 atctcctccg aggggatgcg gatccccacg tcgagcgcga tgagggctcg gcgaccgagc
    19441 agggccacgg acggcaggag gacgcccgcg atcaacaggg tccatcgagg cccgatcgcg
    19501 acgatgatga gcgacgccag gaaggagccg atcccttcgc cggccatgta gagcgattcc
    19561 agcaccccga aggcgcgtga tagctgcttg tccgggatca gccgttgcag catcacccgc
    19621 ccgttgacat cggcgagtgc accacccgcc ccgctcatcg ccaggaaaac gcccgcgagg
    19681 gccggctgcg aggcgatccc ggcgatcgag agcccgctcc acgagatccc ccatcggaac
    19741 gatcgggacg cccgccaccg cgccgcgacg ccgatcgcga gcatcgctcc cacgacggcg
    19801 ccgactccga tcgacgcggt caggaagccg acgcctgcgt cgctcgtgcc gatgacgtcg
    19861 aaggcgagga ccacgatcaa tacgtcgacg aacccgagct ggaccatcgc gagccccatc
    19921 gtcgagacca ggagcagcga ccggcgatcg cgcgccaccg cccggacgcc gtcgacggca
    19981 tcggatgcga cgatccgggc gccggaggtc ccgccgtcgt gcgcgggctc ggtgcggatc
    20041 cggatcgagg ccacgctcac tgtgcccagg aggagcatcg cggcgagggt cgcatccacg
    20101 acgccgggtc ccgagacgga accgggcgtc agacccatca gtccaccggc gaggagcggg
    20161 ccgagcaaga tgctgaagct ctccaggaga ctgctcgcgg cgaaggcact cgagagctcc
    20221 tccggcgtgc gagccagccg cggcaggagg gaggcgtgcg tgggacggac gagcgtgagc
    20281 acccaccccg cggtggtgcc cgacgcgtag accagcgggg ccggtgcgtc ggtgagcaat
    20341 gccacgccgg ccaacatcgt catcgcgccg agcgaggcgt aggagtacac caacacccgc
    20401 tcgcggcggt gccgatcacc gagaacggat ccgaccggcg cgatgagcgc ggcggggatc
    20461 agttgcagga cgctcaccag accgaccatg acgctgccgc cgtcctggta ggcgtacacg
    20521 atcagcgcga tgaacgccgc ccactccgtg atcgtgaacg cgagataggc cgcgagcgcc
    20581 ttggagatgt cgggcgaccg gaacgaccga ccgagcgcgg agaacgacgt ccccacgtcg
    20641 gcgaggaccc gtttcaccgt ggatccgttc tccgaccgat cctcgctcac gaaggcagga
    20701 gtctatgcca ggtcgtccgg gcccgcgttg ggctacagtc gcctccgaag agcgggcgct
    20761 tcgcgcatct gcccgcgcat cacaccacct ctcctgaaag gacctccgca tgtcgcgtcg
    20821 tgtgctgatc atgggagccg ccggccgcga cttccacaat ttcaacgtcg tcttccgcga
    20881 cgacccctcc accgaggtcg tcgcgttcac cgcgacgcag atcccgtaca tcgacgaccg
    20941 gacctatccc gcggggctcg ccggggagcg ctacccggac gggatccgga tccacgagga
    21001 ggacgagctc tcccgcctga tccgccagct ggaggtggac gacgtggtgt tcgcctactc
    21061 cgacgtctcg catgagtacg tgatgcacaa ggcgagcgag gccctcgcgg ccggagcgaa
    21121 cttcctgctg ctggggccgg acgccacgat gctgaagccg gccatcccca ccgtggccgt
    21181 gacggccgtt cgcaccggcg tcgggaagtc acagacgacc cgcgcggtcg ccgcgaccct
    21241 gaaaggggcc ggcaagaagg tggtggccgt gcgccatccc atgccgtacg gcgacctcac
    21301 cgcgcaacgc gtccaacggt tcgccgagct cgaggatctc gatcggcacc actgcacgat
    21361 cgaggagcgt gaggaatacg agccgcatat cacgaccgga accgtgatct acgcgggtgt
    21421 cgactacgcg gcgatcctcg agcaggcgca gtccgagggc gacgtgctcc tgtgggacgg
    21481 aggcaacaat gacctcccct tctacctgcc ggacgtccac atcgcgctgg tcgacccgtt
    21541 acgggtcggc cacgagacga ggtaccaccc cggagaggcg aacctccgcc gcgccgacgt
    21601 gatcctgatc aacaagatgg attcgtccac gcccgagcac gtcgcgcggc tcgaggaaac
    21661 gatcgcacag atgaatccga tggccaccgt ggtgaaagcg aactcgaagg tgacggtgga
    21721 tgagcccggc gcgatcgacg gcaagcgggt gctcgttatc gaggacggcc cgacgctcac
    21781 gcacggggag atgaagatcg gcgccggcgt ggtggcggcg cagcgctccg gcgccgccga
    21841 gatcgtggat ccgcggccgt gggcggtcgg gacgatcgcc gagacgttcg agaagtacga
    21901 cgtcggggcg gtcctccccg cgatgggcta cagccagggt cagctcgacg agatggcgaa
    21961 gatcatcgac gcagcggacg ccgacctggt cgtgatcggg acgccgatcg atctccggca
    22021 cgtgatcgac atccgcaagc ccgccgttcg ggtgcggtac gacctcgacg tgcttccgga
    22081 ctcgccgcag ctgctcgacg tgctgcgtcc ggtgttgtag cagactgcgc cccgagcgga
    22141 gccgctgaag ggagccgccg aacatggaac gcgtcgtcgt cgcgctcggc ggcaacgcgc
    22201 tgctccgacg cggtgcggac gacacgttcg aggagctgta ccgggctgcc cgcgccgccg
    22261 cggagcggct cgcagagatc gccgctgagg ggtgggaggt cgtcgtcacg cacggcaacg
    22321 gcccgcaggt ggggcgcatc ctgatccagc aggaggccgc ggccaagacg gtgtccccga
    22381 tgcccctgga cgtctgtggc gccgagagtc aggggttgat cggctatctg ctccaggtga
    22441 cgatcggcga cgtcttcttc gagcgcggca tggagcgtcc ggtcgccacg gtgctcacgc
    22501 tcacgcgggt ccgatcgcag gacccggcct tccggaagcc gtcgaagttc atcggtccct
    22561 tctacaccgc cgcccaggct cgcaagctgg aggagcaacg gcactgggtg atgaaggagg
    22621 acccgcacgg tgggtggcgt cgggtcgtgc cgtccccgcg tccctactcg atcgtcgaga
    22681 cgccgatcgt gaagcgattg gtcgccgacg gggtgatcgt gatcgccggc ggcgggggag
    22741 gggtgcccgt gatcgagaag gggccacggc tgatcggcaa ggagggcgtg gtggacaagg
    22801 acctggcggc cgcgatcctc gccgccgagg tggaggcgag cacgctgctg atcctcaccg
    22861 acgtgcagaa ggtccaacga gggttcggca cgctctatcc ggacgcgatc gatcgcatga
    22921 ccgccgacga ggccgcggac ctgttggaac gcggggagtt cggcgccggc tcgatggggc
    22981 cgaaggtcca ggccgcgatc gacttcctcg aggccggcgg gacccgcgtg atgatcggtg
    23041 acctcgatga ggcgccggcg gtgctccgcg gcgaggcggg caccgagatc gtcccgtcgt
    23101 gaagggcgcc acgctccgtg tggccacatg gaacgtccac gggatgcgtg ctggcgtgga
    23161 tgcgatcgcc cgggtgatcc gcgccgagca catcgacatc gtggtgctgc aggagtccgg
    23221 ccctcgccgg cgcctccggg ccgtcggcga gaccctcggc atgctggtgt gtgccgaccc
    23281 gtgggtgttc ccgcgccgcc ggatccagaa cgcggtcctc gtccggcggg ggctggccac
    23341 cacggtctcc caccgtctcc atcggttctc ggggggctcg ctcctgtatc cgcgaggcgt
    23401 gttgctcgcc gacgtcgacc accgcgtctc cgtcctatcc gtgcatctcg gtctgagcag
    23461 gagcgaacgg gcgcaccacg tcgaccagct gttggcgatg ctcgaggggt cggccggcac
    23521 cttcgtggtg gggggagatc tgaacgccct cccgggcgat ccgggtccgt cgtcgctcgc
    23581 cgggctcgcc tcggactgct gggaggccgc gggcgagggc gccggcgcga cgtacgcctc
    23641 gcacgctccc acagcgcgga tcgactatct cttcgcggac cgagccttcc ggccgctccg
    23701 ggcctggacc gcagggggca ccgcctccga ccatctgatg gtcgtggcgg agctcgcgct
    23761 cacagagagc ggggaggacg gggcgagcgt ccggggctcc tgaagagctg gatcgccatg
    23821 tacaacccga agagggtgac cgcggccacc aggaaggaca gcaggccgag gaagaaccac
    23881 tcgaggaacc cgttgatcgc gagcacgtcg acatcctacc ggcggggagt cggaggaacg
    23941 cttcggtagg gtggcccgat gcgcctcggg atcatcaccg gcggagggga ttgccccggc
    24001 ctgaacgcgg tgatccgggc ggtggtccgc cgaggcatcc gcgcgcacgg catggagttc
    24061 gtgggcttcc acgacgggtg gcacggagtc atcgacgacg tcagccagcc gctcgggatc
    24121 gccgacacga ccggcatact ccctcgtgga gggacgatcc tcggctcctc gggtgccaac
    24181 ccgtaccgct tcgaggacgg tccggcgagg gtcctggcga cgatcgcgaa gcacgcgctc
    24241 gacgggctcg tggtgatcgg cggcgagggg tcgctcggcg tggcggtccg actcgccgac
    24301 gagggcgtcc ggatcgtggg cgtcccgaag acgatcgaca acgatctgtc ggggaccgac
    24361 gtgacggtgg ggttccacac ggccgtccag gtcgcgaccg atgcgatcga ccggctgcac
    24421 tcgaccgcgg agagccacga ccgagtcatg ctcgtcgagg tgatgggccg gcacgccggc
    24481 tggatcgcgg tgtatgcggg gatggcggga ggcgccgacg cgatcctgat ccccgagcac
    24541 cccttcgacg tcgacggtgt ctgccaccac atccgccatc ggcacgcgag ccggagctcg
    24601 ttctcgatcg tggtggtggc agaaggtgcc gtgccgatcg aggggacgat cgagctcccg
    24661 gagcctcccc tcgacgagaa cgggttcccg aggctcggcg gcctcgcgaa ccacctcgcg
    24721 cccgagatcg agaagcggac cggtttccag acccgcgtga cgatcctcgg ccacgtccag
    24781 cgagggggtt cgcccgtctc gttcgaccgg gtgctggcga cgcgattcgg gatcggcgcc
    24841 gcggatctcg ctgccgccgg gggatggggg cggatggtcg cgcggaaggc ccaggaggta
    24901 cgcgacgtgc cgatcgccga cgctgtgacg gagcgcaacg tggtcccgct ggagctgtac
    24961 cgagaggccg aggtcttctt cgggtaggac ggcccgggcc ggttgtaggg tcgacgcatg
    25021 cgaatcggca tcctcacggg aggcggcgac gtccccgggc tgaacgcgtg catcaaggcc
    25081 gccaccaccc gcgccgtcgg cgatggacac gagatggtcg ggatccgtcg cggctgggca
    25141 gggctgctgg agctcgatcc tgcggacccg tcctccacct cgacgcacac cctggccctc
    25201 gatcccgacg tggtccgaac gatcgaccga accggcggca cgatcctcca cacgtcgcgc
    25261 acgaacccgg cgaaggtccg cgccgccgac gagccggact tcctgaaggc cggacgcgtc
    25321 aacgacgagg ccagggacca cacgtcccac gtgatgaagg tgctggacca cctcgggatc
    25381 gacgcgatga tcccgatcgg tggcgacgac acgctctcgt tcgcgctccg gctccacgac
    25441 gagggcgtgc ccgtggtggc cgtcccgaag acgatggaca acgacgtcca cggcaccgac
    25501 tactgcatcg gcttctccac cgcggtcacc cggacggtga tcttcatcca ccagctccgc
    25561 acgagtgccg ggtcgcacga acggctcgcg gtcgtcgagg tcttcggccg atacagcggg
    25621 gagacctcgc tggtgtcggc ctacctctcc gggatggacc gggcgatcat cagcgaggtg
    25681 ccgttcgacg tcgagaagct cgccacgttc ctgatggacg acaagcggaa gaacccgtcg
    25741 cggtacgcga tgaccacgat ctcggagggc gcgcatttcg tcgggggcga gatggtgatg
    25801 ggcgggaccg cggacgcgta cggccatcgc aagctcggcg ggatcggtgc ccagaccggc
    25861 gaggcgctga aggagatcac cggggagggg atcatctacc agcagatcgg ctatctgatg
    25921 cgctcgggcc agccggattc gctcgacctg atggtggcga tcaactacgc ggtgatggcg
    25981 gcggacctgt tgctcgaggg cacgcacggt cggctcgtgg cgctccggaa cggcagttac
    26041 gtgaacgtgc cgctcgaggt cgtccggcag ggcgtcaagc gagccgacgt ggacgcgctc
    26101 tacgacgccg acgagtaccg cccgaagatc cgccacgtcg acggcaagcc gatgttcttg
    26161 tactgaggtc ggcggagccg acggagcccg agcaccgccg atacactccg cggccatgat
    26221 ctccctccgg gtcgacctcc gttccgacac ggtcacgaag ccctccgagg cgatgcgtcg
    26281 cgcgatggcc gaggctgagg tgggggacga ctggtacgac gacgacccga ccgtgaacct
    26341 gctccaggag cgttgcgcgg aagccgtcgg caaggaagcc ggcgcgttcg tcccgaccgg
    26401 gacgatggcg aaccagatcg ggctgcgact gcacttcacg ggaccaggac acctcgtcgc
    26461 ggcggcggcc ggcctgcacg tcacgagcgt cgaggtgatg acggcagcgg cgttggccgg
    26521 catcgcctac cggacgatcg aagctggccc tcgcggctgg atcgatgccg agcaggctgc
    26581 gcatctcctc gaacccgacg agttctacga cgtggaggtg atcgacctcc tgtccgtgga
    26641 gaacacggtc ggcggggccg gcgggcgcgt gatgccgatc gaggagctcc gtgcggttcg
    26701 gaaggtcgcg aacgacgccg gcgtgccgat ccacctcgac ggcgcccgca tcttcaacgc
    26761 ggcagccgct gccgaggtcg acgccgccga catcgccgcg gaggcggaca ccgtgatgtt
    26821 ctgcctctcc aagggcctcg gcgctccgat cggctccgtc ctgtgcgggc cggccgacat
    26881 ggcgcgggag gctcggcgcc tgaagatcct gtacgggggc gcctggcggc aggccgggat
    26941 cgtggccgcc gcgggcctga tcgcgctcga ggagggaccg aagcgcctcc acgaggatca
    27001 cgagcgggct cgccagctcg ccgcaggggt cgccgaggcg ttccccggct ccgtcgacct
    27061 ggaacaggtt gaaacgaaca tggtcctcgt cgataccgaa gccgtggggg tgccgatgct
    27121 cgaggcgatc gagcggctgg cagccctcgg ggtgggcacc acgcattcgg gcgggaagat
    27181 ccggatggtg acgcacctcg atgtcgatga caccggcgtc gcggtcgcgc tggatgcctg
    27241 gcgcgagctc gcgtcggaga gaggacgctg atggccctgt tctcgaagaa ctaccggccg
    27301 gagatcgcct cgcgcatccc gccggggcag cggctggtga agagctggcc ggtcctgcat
    27361 tacgggccga tcccgacgtt cgacggcagc aactgggacc tggagctgtc agggctcgtg
    27421 gagaaccccg tgacgctctc gtaccaggag ctccgcggcc tgccgaacgt cgatgtgccg
    27481 gccgacatgc actgcgtgac cggctggacg acgctggaca actcctggca cggggtctcg
    27541 ttccggacgc tgctcgacct cgcgaagccc gggccggaag cgaagtgggt gatcgcccat
    27601 tgcgagtacg gctacacgtc gaacctctcg ctcgaggcga tgaccgatga tgacgtcctg
    27661 gtcgcgtggg cgaacggggg acgggacctc gagccggagc acgggtggcc gctccggctc
    27721 gtcgtgccga agcggtacgc ctggaagagc gcgaagtggc tcaccggcct ggagttcgtc
    27781 gacgagaaca agcggggctt ctgggaggtc cgcggctacc acgtccacgc cgagccgttc
    27841 ggcgaggaac ggtactccta ccaggagggc cctcgagccg agctcgagcc ctaggaccga
    27901 agaccggcag gtcccctcct cagcgcggct ggcagctcgg gcagagatag gtcgttcggc
    27961 cgccgacgac ggtccgcgcg agctcggtgc ggcaccgagg acatcgaccc tcgccccgtt
    28021 gccgtgcatc gttccatcgt gatcggaacc cgtcgggata gctgtccacc gtctggcggt
    28081 ggatcaccga gcggagctga cggaagagcg tgatccggtc atcgatgggc agggacgcga
    28141 tcaggcgagc cgggtggatc cgagccctcc agagcgcctc gtcggcaagg aggttgccca
    28201 cgccggcgat gaacgactgg tcgaggagga ccgccttgat cccgccacgg cgggtctcca
    28261 ggagccgcag gaagctcgct cggcctagat cgagcgcgtc agggcccagc cgggccagaa
    28321 ggccctcgac ctcgtcccgg ttgccggcca gccacacgcc cccgaacttc cgcatgttcc
    28381 ggtagcggat ctccccgtgg tccaagacca cgatcacccg gtcgtgcgcg tgtcgatccg
    28441 cagcgtcggc cgcccacagg atgtctccgg tcatcccgaa gtgcatcacg agcgcgggtc
    28501 cgtcggtcca ggcgatcacc cacttccctc ggcgctccgg ctcttcgaag cgatggccac
    28561 ggagcgcctg gtcgagctcc gccgcggtca cgtctcgcag gatcccgggg tcggtcacga
    28621 caacgtcgga cacgcggtga cccgcggcgc cacgggcgaa cgtccgtcgg aaatgctcga
    28681 cgtccggaag ctccggcacg gcggactatg atggggtacg ccgaacgcgg gtcctcagga
    28741 tgtcccggaa gctcacgatg cccgcgatgg tcgtcccctc caccaccggc aggtgtcgga
    28801 aaccctgact catcatgatc tccgtggcct cacccgtgtc catgtcgggg ctcgcggtga
    28861 ccggatctcc ggtcatccag tcggccaccc gggacgacgt caggtcgctt cgtgcagcgg
    28921 ccgcgcggag aacgtcgcgt tccgtgaaga tccccatcaa cgtggatccc tccatcacca
    28981 ccgccgaccc gaacctgccc ttcagcatgc tggctgcgac gtcggcaacg gatgcgctcg
    29041 gtgtggtcgc gaacacgtcc gacgtcatga tctccctcag cgtggccacg ttcgctcagg
    29101 cctccatctc agccgccgcc ctcgccgccg acctcgtggg tctccaggtc cccggcaggg
    29161 gtggcgggat cggccaggtc gtcgttcagt tggcgccaga gcgcgccgcc ccacgagctc
    29221 acctgcggcc agcgttgcca gaagagctgc agggtcgtct cgtcgaaggt cctggttgcg
    29281 tcgtcgggag tgacggcctc tgccactcgc tcgtagtcct cgatgatccg caggagccga
    29341 tcggaagcgt cgtccacagg cacctcctcc cgcggcggag tctaaacggg agccgcgagg
    29401 ccccgcagat aggtgatgtc cgtccgttgg cgatcggcat ctccgggcgt ctcgagcatc
    29461 cagggaacag agccgagcac gggatcgtgc accaacgccc gccagccctg ctcaccgatc
    29521 ttcccgtctc cgatgtcggc gtggcgatcg cggcgggcgc cgcgctcgaa caccgagtcg
    29581 ttcgcgtgga ccagggccag tcgccggtcg agcgcgcgca cgcggagctc ctccgcgaac
    29641 ccctccaccc cctccgcgct gtcgaggggg acaccggccg cgaacaggtg cgcggtatcg
    29701 aggcagatgc cgagtcgctc atcgtccacc gccgtgcaga gctcctcggc ctcgctgggg
    29761 atcgaggcaa cggcgccgct cgtgccggcc atcagctcga ccaacacacg cgtccggtcc
    29821 gcttcgccga ccgccagccg gaagctctcc gcggccgctc ccaacgccgt ctcccgatcc
    29881 tgcggcccgc cggctccggc atggaccacc aggagatcca cccccagggc gtcgcacgcg
    29941 tgcaccgttc ccacgaggag ctcacgggtc cttcgcaacg tcgtggggtc ccaggacgcg
    30001 acgttgccca cgtaggacag gtgtgccacg cacggccgca gcccgctcgc cgtgagggca
    30061 tcgaggaacc gctcgatctg tggtccctcg taccgggggg cggcccacgc ccgcgggttg
    30121 gagacgaaga tctggaggca gtcggcgccc cgcagcgcgg cctcctcgat cgctccctcg
    30181 atgccggtgt gcctgcgccg gacgtgggcg cccaggatca cgcgtagtag acggtgatcg
    30241 tggagccgta acgaacgcgc gtaccggcgg gcgggagctg actcacgatg ctgccgccgc
    30301 tgctgccggg gacggggagc gcggtgagct ccagcccgac gtcctccgcg agcgcgcgtg
    30361 cggcatcgac cgtcatgccc tcgaagtcgg gacaggcgaa gaactcgggc ccgagcgaga
    30421 cccgcaacac gatcgtctca ccgggctgga gctcggtccc gctctccgga tcggttccga
    30481 gcgtccggcc gcgcgcgacc ttgttcgaga agtcctcctc gatcacgacc tcgaagccct
    30541 tcgtggtgag cgccttgacc gccttgtccc ccgtcatgtc gccgacgtcg gggatcgcga
    30601 ccggcttcgg gccgtcgctg acgatcaccg tgaccgacgt ccctcggggg agcctcgggg
    30661 cgtcgggttg cacgccgatc acgtgaccct cggggacctc ccgatcgaac cgatgtctga
    30721 cggcccccac gtgaaagccc gccccggcga tcccctcgcg cgcgtcctgg agaggctccc
    30781 ctcgcacccg ggggacgtcc accggcggcg gcccgagcga cgggacgatc gtgaccgtgg
    30841 tgccgacatc gcgctcggcg ccggcggccg gcaggatctg cttcacgtgt ccgtcgggaa
    30901 cctgcatgga gtactcgccg ggggctcgcg tgacgtcgaa cccgaggccc gtcagccggt
    30961 ccgtcgcgtc atcgacggcc tcaccgacga gcgcggggat cacggccgta tgcgggatcg
    31021 cgtacgtcca ggtgccccac gcggccgccg tcagcagcgc caccagtgcg acagcgccca
    31081 ggacgcgctt gacgaacctc cggcgcttcc ggtggcgctc catgcggggg atggaggtct
    31141 cgagcctcac ccccacctcg gtcgtctctc ctccctccac cgtcacggag gggatctccc
    31201 gaacgatcgt ggagagcttc cgtgccgggg gaagctggcg cgagagcaca tcgaggtcca
    31261 tccgcatcac gtcggcggac tcgggtcgga gctcccggtc gcgatcggtc gcggcggcca
    31321 cgaagccgtc cagctcggcc ggcaggtccg ggaggacgga cgtcggggcg ggcacgcggt
    31381 ccgagaggtg cttgtaggcc acggccatcg cggtctcgcc ggtgaacggg agcttcccgg
    31441 tcaggagctc gtaggcgacg atgccgaggg agtagaggtc gcttcgcgga tcggccggct
    31501 cgccgcggat ctgctcgggc gccaggtact gcaccgtccc ggtgaccgcc ccggcctggg
    31561 tcacgcggcc gtcggcgtac gctcgcgcga gcccgaagtc ggcgaccttg acca
//
LOCUS       DASUYD010000013         7807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_449373, whole genome shotgun sequence.
ACCESSION   DASUYD010000013 DASUYD010000000
VERSION     DASUYD010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7807
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449373"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..993
                     /locus_tag="VF195_00675"
     CDS             <1..993
                     /locus_tag="VF195_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579381.1"
                     /translation="FFWEGRAGRFPMGMYGAEVVQVIPDPSQVFGAFKIANLTELHGI
                     IDQDRLTGIRGVEGIEPTSVPVTSIVSNLDTATIRAGETTVFRQDTDSFPWLPDIAAF
                     ALLANEDVVYDRIGDGSATLRFVLEGTGPDGVPFSLERNNMYFSDFDITFESIIELFS
                     FLYQIQVSEFGDVAFTSVDTEASLTQEQLTSEIVRLRVASSLQPALRERDRLRVRPGD
                     TIRLEVSLLAEGALEADAVELLVPVPNTRFGGRLVVQGGGADSCLFCFFDEENGEDEG
                     PATFAQLLRQLRRTPRNNDLIGTLEVGRNARRTSTFRTDRVILGEEQVEIVVVR"
     gene            complement(1033..2517)
                     /gene="nuoN"
                     /locus_tag="VF195_00680"
     CDS             complement(1033..2517)
                     /gene="nuoN"
                     /locus_tag="VF195_00680"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003927353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEX6579382.1"
                     /translation="MIGQAALPTPELVFEPLLPELVLVVFAMAGLLYEAFAKRAEAVA
                     HLSIGLAGVVLAGATSLWLWDWDGPATVLADAVSTDRFAVLARLLLLVVAALGLLLGH
                     QYFARSGDEQRGEFTPLLLFATSGMTLIVASADLILTFLALEILSLTLYVLTGLTGRR
                     AANEAAMKYFLLGAFSSAFFLYGVTMAYGATGTTKIAAMALALTGTGDAQALAVLGFA
                     LLAVGFAFKVSAAPFHMWTPDVYQGAPTPITAFMSAATKVAAFGALIRVLDVAFQALA
                     WDWAPVVWGLAAASVVIGSILAIAQTNVKRMLAYSSIAHAGFILTGLTAAGSAGIRAA
                     MFYLVAYAAMSVGAFGTVMVVSGRGEERTRIGDYTGLARTNPIAAALMTLFLLSLAGI
                     PPTAGFIAKVNVFSAAIDAGYWVLALIGVLASVAAAFFYLRVIVLMYMQEPETAPAPA
                     GIAVVPATGATGAILAIPAAATLLLGVFPGVIVGIIEEASVLRW"
     gene            complement(2514..4061)
                     /locus_tag="VF195_00685"
     CDS             complement(2514..4061)
                     /locus_tag="VF195_00685"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6579383.1"
                     /translation="MNKLLTLTTFLPLIGVLMMLGGGRGLKDRAARWVALGVSLATFI
                     VSLVVLGRFDPATSESFQMVEIARWVPSIGLSYAVGIDGVSIWMLLLTTFLFPVSILA
                     SWTITKDVRRYLAAILVLETAVIGTFVALDLLLFFLFFEALLVPMYLLIGGWGGQRRI
                     YAAVKFFLFTMAGSAFLLVAILFLYAQQEGPRLFGYAAMMNAAADVPVTTARWLFLAF
                     FVAFAVKVPLVPLHTWLPDAHTEAPTAGSVLLAALLLKVGTYGLIRFNLMLFPEASRY
                     FATFVGVIAVIGIVYGAVNALVQTDIKRLVAYSSVSHLGFVVLGVFAFTQQGVTGGVL
                     QMVNHGLATGALFLLVGMVYERTHTRDLDSMGGLASVMPWLMGAFLFTAFASAGLPGL
                     NGFVGEFLVIVGTFAVSTWFGALAATAVVLAAIYLLWSYQRMAFGPVREEHRLLPDVS
                     LREVVVLAPVLALLLLFGVAPKLLTDRIDPATEAVIARVAPDHRTDVGVREQALLIPP
                     LDGAAAP"
     gene            complement(4058..5959)
                     /gene="nuoL"
                     /locus_tag="VF195_00690"
     CDS             complement(4058..5959)
                     /gene="nuoL"
                     /locus_tag="VF195_00690"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6579384.1"
                     /translation="MEATKGPEGFTIIWLVPLIPLAIATCNLFLGRRIERLAGILAAG
                     AVGLSFVFSLVLVVDLLGLPPDARIRITHFFDWISVGRFSVGADLRLDVLSATMILVV
                     TGIGFLIHVYAIGYMADDPRFGRFFAYLNLFVFFMLLLVLADNYLLLYVGWEGVGLCS
                     YLLIGFWFEKTENASAAKKAFVTTRIGDTAMLIGLALIVSKFGTLDFDAVLGAPGDAI
                     AKGTATAISLLLFAGAIGKSAQVPLHVWLPDAMAGPTPVSALIHAATMVTAGVYLVVR
                     SAPLFELSGAALTVVLIVGLVTALFAATCAIAQDDIKRVLAYSTVSQLGFMFMAAGMR
                     FYTGAMFMLVAHAFYKALMFLGAGSVMHGMHGETDLQRMGGLIRRMPITGWAFVVGAF
                     ALAGVFPLAGFFAKDQILEIANHTGRTWIYLLGTLGALLSALYIGRLAFLAFFGSPRS
                     EEAEHAHESPSVMTIPLLVLAAGAVLAGLFLSTSAEGALARVLEPVTGPVPAGEGLST
                     LALSGIATAIALGALAVAWWVYASGRVEWLTLRERLQPLPRAARSGWYVDRAYSTLFV
                     DPGAAAARFTANVFDTKVVDGLVNGVGAGTRRLAAVGRLLQTGFVRSYALAFLLGAVG
                     VLVWVGTRL"
     gene            complement(5959..6264)
                     /gene="nuoK"
                     /locus_tag="VF195_00695"
     CDS             complement(5959..6264)
                     /gene="nuoK"
                     /locus_tag="VF195_00695"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007320414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6579385.1"
                     /translation="MRTPIAYFIVLSGVLFTLGTLGVLLRKNALLIFMSVELQLNAVN
                     LALVAFSRMHLALDGQVLAFFSMVVAAAEVVVGLAIIVAVYRRRGTVDVDEARGLAG"
     gene            complement(6261..6833)
                     /locus_tag="VF195_00700"
     CDS             complement(6261..6833)
                     /locus_tag="VF195_00700"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005165.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6579386.1"
                     /translation="MPLDNVVFWVFAPISVGSAVGMLLMRNAVHAALFLVVNFFCLAV
                     FFLILDAPFLFAVQIIVYAGAIMVLFLFVIMLLGVDRGDDLRERLVAQRPLALALAAG
                     FIIEITLAVRTGIGFATKAPGGFDAVNDPGNAQAVATVLFREYFLPFEVTSVLLIIAA
                     IATMVLGQRKARAVTEAELQAMGAMKEAGA"
     gene            complement(6836..7336)
                     /gene="nuoI"
                     /locus_tag="VF195_00705"
     CDS             complement(6836..7336)
                     /gene="nuoI"
                     /locus_tag="VF195_00705"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEX6579387.1"
                     /translation="MTDERRRERNFLADTGAVLKGMATVFKQTFRPDDTEEYPKVIAE
                     IPGRAHTGRHRLNRHDDGLEKCIGCELCAFACPADAIWVEGEDNDPEDPTSPGERYAK
                     DYQINYLRCIMCGLCVEACPTRALTLTSYFEMAYTSREDAILTKEQLLEPPPELGTTP
                     GAKEGL"
     gene            complement(7333..>7807)
                     /locus_tag="VF195_00710"
     CDS             complement(7333..>7807)
                     /locus_tag="VF195_00710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HEX6579388.1"
                     /translation="DLAEAETELVAGFHTEYSGITFAMFFLGEYVSTVTVAAIGVTLF
                     LGGWRGPAPDFLPALWPFLWFLLKVTLVIYLFVLIRGTLPRVRYDRLMAFGWKVLIPF
                     GLVWVLITGAIVVLPTELELDRGTVLTWAAVALGATLLLSFLPIGRTSRREEVPR"
BASE COUNT         1484 a         2679 c         2657 g          987 t
ORIGIN      
        1 ttcttctggg agggtcgcgc tggccggttc cccatgggga tgtacggcgc cgaggtcgtg
       61 caggtgatcc ccgacccgag ccaggtcttc ggcgcgttca agatcgcgaa cctcacggag
      121 ctgcacggca tcatcgacca ggatcggctg acagggatcc gcggcgtgga gggcatcgaa
      181 ccgacgtccg tgccggtgac ctccatcgtg tcgaacctcg acaccgcgac gatccgggca
      241 ggggaaacca cggtcttccg tcaggacacg gacagcttcc cgtggctgcc ggacatcgcc
      301 gcattcgcgc tcctcgccaa cgaggacgtg gtgtacgacc ggatcggcga cggttcggca
      361 accctccggt tcgtgctcga aggcacgggg cccgatggcg ttccgttcag cctggagcgg
      421 aacaacatgt acttctcgga cttcgacatc accttcgaga gcatcatcga gctgttctcg
      481 ttcctctatc agatccaggt gagcgagttc ggggacgtcg cgttcacgag cgtcgacacg
      541 gaggcctcgc tcacccagga gcagctgacc tcggagatcg tccggttgcg ggtggcgagc
      601 tcgctccagc ctgcgctgcg cgagcgagat cggctccgcg tgcgacctgg cgacacgatc
      661 cggctggagg tgtcgctcct cgccgagggg gcgctcgaag cggacgccgt ggagctgctc
      721 gtgcccgtgc cgaacacgcg gttcggcggc cgcctcgtcg tccagggcgg gggggccgac
      781 agctgcctct tctgcttctt cgacgaggag aacggcgagg atgagggccc ggcgacgttc
      841 gcgcagctgc tccgtcagct gcggcgcacc ccgcggaaca acgacctcat cgggacgctc
      901 gaggtcggcc gcaacgccag gcgtacgtcc accttccgca cggacagggt gatcctggga
      961 gaagagcagg tcgagatcgt cgtggtccgc tgaggaccct gggtcgggaa ggatgaggga
     1021 cgccggtgtc cctcaccacc tcaggaccga cgcctcctcg atgatgccca cgatcacgcc
     1081 ggggaagacg ccgaggagga gcgtggcggc ggccgggatg gccaggatgg ctcccgtggc
     1141 accggttgcg ggcaccacgg cgatgcccgc cggcgccggc gcggtctccg gctcctgcat
     1201 gtacatcagc acgatcaccc gaagatagaa gaaggcggcc gccacgctcg ccagcacccc
     1261 gatcagcgcc aggacccagt acccggcgtc gatcgcggcc gagaagacgt tcaccttggc
     1321 gatgaacccc gcggtcgggg ggatcccggc gagggacagc aagaagagtg tcatcagggc
     1381 ggccgcgatc gggttcgtgc gggcgagacc ggtgtagtcg ccgatccggg tccgctcctc
     1441 cccgcgaccg gacacgacca tcacggtgcc gaacgcgccc acggacatcg ccgcgtacgc
     1501 caccaggtag aacatcgcgg cccggatgcc cgccgaaccc gccgccgtga gccccgtcag
     1561 gatgaacccg gcgtgcgcga tgctcgagta cgcgagcatc cgcttcacgt tcgtctgcgc
     1621 gatcgcgagg atgctcccga tcacgaccga ggcggccgcg agcccccaga cgaccggcgc
     1681 ccaatcccac gcgagagcct ggaacgcgac gtccaggacg cggatcagcg ccccgaacgc
     1741 ggcgaccttc gtcgcggccg acatgaaggc ggtgatcggt gtgggtgccc cctggtacac
     1801 gtccggcgtc cacatgtgga acggagcggc cgagaccttg aacgcgaacc ccaccgcgag
     1861 cagcgcgaac cccagcacgg cgagcgcctg ggcgtccccc gtgccggtga gggcgagcgc
     1921 catcgccgcg atcttggtgg tgcccgtcgc accgtaggcc atggtaacgc cgtagaggaa
     1981 gaacgccgag ctgaacgcgc cgagcaggaa gtacttcatc gcggcctcgt tcgccgccct
     2041 gcgtccggtg agccccgtga ggacgtagag cgtgagcgac aggatctcga gcgcgaggaa
     2101 cgtcaggatc aggtccgccg aggccacgat cagcgtcatc ccggacgtcg cgaacagcag
     2161 caagggcgtg aactcgcctc gctgctcgtc gccggacctc gcgaagtatt gatggccgag
     2221 gagcaggccg agcgcggcga cgacgagaag gagcagccgc gccaggacgg cgaaacggtc
     2281 cgtcgacacc gcgtcggcca gcaccgtggc cggcccgtcc cagtcccaga gccagaggct
     2341 ggtcgcgccg gcgaggacca cgcccgcgag cccgatcgag agatgcgcga ccgcctcggc
     2401 ccgcttcgca aacgcctcgt agagcagtcc cgccatcgcg aacaccacga gcacgagctc
     2461 gggcagcagc ggctcgaaga cgagctcggg cgtcgggagc gctgcctgac cgatcacggc
     2521 gcggccgccc cgtcgagcgg agggatgagg agcgcctgct cccggacccc gacgtcggtc
     2581 cgatggtcgg gagcgactcg agcgatcacg gcctcggtcg ccgggtcgat gcggtccgtc
     2641 agcagcttcg gcgccacgcc gaagagcagg agcagcgcga gcaccggcgc cagcaccacg
     2701 acctcgcgaa ggctcacatc gggcaggagc cggtgctcct cgcggaccgg cccgaacgcc
     2761 atccgctggt acgaccagag gaggtagatc gcggccagca cgacggcggt cgccgcgagg
     2821 gccccgaacc aggtgctcac ggcgaacgtg cccacgatca ccaggaactc gccgacgaac
     2881 ccgttcaggc cggggagtcc ggcggaggcg aacgcggtga acaggaatgc gcccatgagc
     2941 cacggcatca ccgaggcgag cccgcccatg ctgtccaggt cgcgggtgtg cgttcgctcg
     3001 tagaccatgc ccaccaagag gaacagcgcg ccggtggcga gcccgtggtt caccatctgc
     3061 agcacaccgc cggtgacccc ttgctgcgtg aacgcgaaca cccccagcac aacgaagccc
     3121 aggtgggaga cggacgagta cgcgacgagc cgcttgatgt cggtctggac cagcgcgttc
     3181 accgcgccgt acacgatccc gatcacggcg atcacgccga cgaaggtcgc gaagtacctc
     3241 gacgcctccg ggaacagcat caggttgaag cggatcagcc cgtaggtccc gaccttcaac
     3301 aggagcgccg cgagcaacac ggagccggcc gtcggggcct cggtgtgcgc gtccggcagc
     3361 catgtgtgga gcggcacgag cggcaccttc accgcgaacg cgacgaagaa cgcgaggaac
     3421 agccaccggg ccgtcgtgac cggcacgtcg gctgcggcgt tcatcatcgc ggcgtagccg
     3481 aaaagtcgcg ggccctcctg ctgcgcgtag aggaacagga tcgcgacgag caggaacgcc
     3541 gaacccgcca tcgtgaacag gaagaacttg accgcggcgt agatacgacg ctggccgccc
     3601 cacccgccga tcagcaggta catcgggacg agcagagcct cgaagaagag gaagaacagc
     3661 aggagatcga gggcgacgaa cgtgccgatc accgcggtct cgagcacgag gatcgccgcg
     3721 aggtagcgac ggacgtcctt cgtgatcgtc caggacgcga ggatcgagac ggggaacagg
     3781 aacgtcgtga gcaggagcat ccagatgctg acgccgtcga tgcccaccgc gtaggagagg
     3841 ccgatcgacg gcacccagcg cgcgatctcg accatctgga acgactccga ggtcgctggg
     3901 tcgaaacgcc cgagcacgac gagggacacg atgaacgtcg cgagcgagac gccgagggcg
     3961 acccatcgtg cggcccggtc cttcagcccg cgacccccac ccagcatcat cagcacgccg
     4021 atcagcggga ggaacgtcgt gagcgtgagc agcttgttca caaccgggtt cccacccaga
     4081 cgaggacgcc gacggcaccg aggaggaacg cgagcgcgta ggagcgcacg aagcccgtct
     4141 ggagcagccg accgaccgcg gcgagccggc gcgtcccggc gccgaccccg ttgacgagtc
     4201 cgtccacgac cttcgtgtcg aagacgttcg cggtgaaccg ggccgccgcg gccccgggat
     4261 ccacgaacaa cgtggaatag gcccgatcga cgtaccagcc gctccgcgcc gcgcgcggga
     4321 gcggctggag ccgctcgcgg agggtgagcc actcgacccg gccggacgcg tagacccacc
     4381 acgcgaccgc gagcgcgccc agcgcgatcg cggtcgcgat ccccgacagg gcgagggtcg
     4441 acagcccctc gcccgccggc accggaccgg tgacgggctc gagcacccgc gccagggccc
     4501 cctccgccga ggtggacagg aagagaccgg cgaggacagc gccggccgcg agcacgagga
     4561 gcgggatcgt catgaccgac ggcgactcgt gggcgtgctc ggcctcctca cttctcggcg
     4621 atccgaagaa cgcgaggaac gcgagccggc cgatgtagag cgcggacagg agcgcgccga
     4681 gcgtccccag gaggtagatc cacgtgcgcc cggtgtggtt cgcgatctcg aggatctggt
     4741 ccttcgcgaa gaagcccgcg agcgggaaca cgccggccag cgcgaacgcg cccacgacga
     4801 atgcccaccc cgtgatcggc atccgtcgga tcagcccgcc catccgttgc aggtcggtct
     4861 ccccgtgcat gccgtgcatc acggagccgg cgcccaggaa catcagcgcc ttgtagaacg
     4921 cgtgagcgac gagcatgaac atcgcgccgg tgtagaagcg catgcccgcc gccatgaaca
     4981 tgaatccgag ctggctcacc gtcgagtagg cgaggacgcg cttgatgtcg tcttgggcga
     5041 tggcgcacgt cgcagcgaat agcgccgtta cgagcccgac gatcaacacg actgtcaagg
     5101 ccgcgccgga gagctcgaag agcggcgcgc tccggacgac gaggtagacg cccgccgtga
     5161 ccatcgtcgc ggcgtggatc agcgccgaga ccggcgtggg gccggccatg gcatccggaa
     5221 gccaaacgtg cagcggtacc tgcgccgact tgccgatcgc ccctgcgaac agaagcaggg
     5281 agatggcggt ggcggtcccc ttcgcgatcg cgtcccccgg agcgcccaga acggcgtcga
     5341 agtcgagcgt gccgaacttc gacacgatca gggcgagtcc gatcagcata gcggtgtcgc
     5401 cgatccgggt cgtgacgaac gccttcttcg cggcgctcgc gttctcggtc ttctcgaacc
     5461 agaagccgat cagcaggtag gagcagagcc cgacaccttc ccagcccacg tagagcagga
     5521 ggtagttgtc ggccaagacc agcaggagca tgaagaagac gaagaggttc aggtacgcga
     5581 agaaccgccc gaagcgagga tcgtccgcca tgtagccgat cgcgtacacg tggatcagga
     5641 aaccgatacc ggtgacgacc aggatcatcg tcgccgagag cacatcgagc cggagatccg
     5701 cgccgaccga gaaccggccg accgagatcc agtcgaagaa gtgggtgatc ctgatgcgcg
     5761 catcgggcgg cagccccagc agatccacga ccaggacgag cgagaacacg aacgacagcc
     5821 cgaccgcccc tgcggcgagt atccccgcga gccgctcgat gcgtcgacca aggaacaggt
     5881 tgcaggtcgc gatcgcgagg gggatcagag ggacgagcca gatgatcgtg aagccttccg
     5941 gacccttcgt ggcctccatc agcccgccag acctctcgct tcgtcgacgt cgacggtgcc
     6001 gcgacgccgg tacacggcca cgatgatcgc cagaccgacg accacctccg ccgccgcgac
     6061 caccatcgag aagaacgcca gcacctggcc gtcgagcgcg aggtgcatcc gcgagaacgc
     6121 caccagggcg aggttcaccg cgttcagctg gagctcgacc gacatgaaga tcagcagcgc
     6181 gttcttccgg aggagcacac ccagcgtgcc cagcgtgaag agcacgccag agaggacgat
     6241 gaagtacgcg atcggcgtcc tcacgcgccc gcctccttca tggcgcccat cgcttggagc
     6301 tcggcctccg tcaccgcgcg ggccttccgc tgaccgagca ccatcgtcgc gatcgccgcg
     6361 atgatcagca ggacggacgt cacctcgaac gggaggaagt actcgcggaa cagcaccgtg
     6421 gcgaccgcct gcgcgttccc gggatcgttc accgcgtcga agccgccggg cgccttcgtc
     6481 gcgaacccga tccccgtccg cacggcgagg gtgatctcga tgatgaaccc ggcggccagg
     6541 gccagcgcga gcggccgttg cgcgaccagt cgctcgcgga ggtcgtcgcc acggtccacg
     6601 ccgagcagca tgatcacgaa caggaacagc accatgatcg cgcccgcgta gacgatgatc
     6661 tggacggcga acaggaacgg cgcgtcgagg atcaggaaga agaccgcgag gcagaagaag
     6721 ttcacgacga ggaacagggc ggcgtgcacg gcgttgcgca tcaagagcat cccgacggcc
     6781 gaccccaccg agatcggggc gaacacccag aacacgacgt tgtcgagcgg cacccctaca
     6841 gcccctcctt cgcccccgga gtggtgccga gctccggggg tggctccagg agctgctcct
     6901 tcgtgaggat cgcgtcctca cgtgaggtgt aggccatctc gaagtagctc gtcagggtga
     6961 gcgcgcgcgt ggggcaggcc tcgacgcaga ggccgcacat gatgcagcgc aggtagttga
     7021 tctggtagtc cttcgcgtag cgctccccag gcgacgtcgg atcctcggga tcgttgtcct
     7081 ccccctccac ccagatcgcg tcggccgggc aggcgaacgc gcagagctcg catccgatgc
     7141 acttctccag gccatcgtcg tgccggttca gccggtggcg accggtgtgc gcccgtcccg
     7201 ggatctccgc gatcaccttc gggtactcct cggtgtcgtc gggacggaac gtctgcttga
     7261 agaccgtcgc catgcccttc agcacggcgc ccgtgtccgc caggaagttc cgctcccggc
     7321 gacgctcgtc cgtcatcggg gcacctcctc acgtctcgac gtgcggccga tcggcaggaa
     7381 cgagaggagc agcgtcgcac cgagcgcgac cgcagcccac gtcaacacgg tcccccggtc
     7441 gagctccagc tcggtgggca gcaccacgat cgcgccggtg atgagcaccc agacgagccc
     7501 gaaggggata aggaccttcc agccgaacgc catcagccgg tcgtaacgga cccgtgggag
     7561 cgtgccgcgg atcaggacga acaggtagat cacgagcgtg accttcaaca ggaaccacag
     7621 gaacggccag agggcaggga ggaagtccgg cgccggccct cgccaccctc cgaggaacag
     7681 cgtcacgccg atcgccgcga cggtcacggt cgagacgtac tcgccgagga agaacatcgc
     7741 gaacgtgatc cccgagtact ccgtgtggaa gcccgccacg agctccgtct cggcctcggc
     7801 gagatcg
//
LOCUS       DASUYD010000014         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1773140, whole genome shotgun sequence.
ACCESSION   DASUYD010000014 DASUYD010000000
VERSION     DASUYD010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773140"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /gene="pilO"
                     /locus_tag="VF195_00715"
     CDS             complement(<1..434)
                     /gene="pilO"
                     /locus_tag="VF195_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX6579389.1"
                     /translation="MNRRAPIIAGAVLLVALLAVFLFILPKIDEVGETEDRLQEAQDQ
                     EIDLAAQLKALEDARAAAPETELEIAAIDTQVPPTAGLPSVFRLLQGAADRASVDFFS
                     FTPGTPVPNPTGSYSTIESQITVSGRYFAIDEFLFLLETLKRA"
     gene            complement(431..1069)
                     /locus_tag="VF195_00720"
     CDS             complement(431..1069)
                     /locus_tag="VF195_00720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579390.1"
                     /translation="MSLVNLLPPELRERQTVRRTTSLVVGPGLAALALIGIFFFFQVQ
                     RLSQVRSDLEAQQSTNARLVRQIASLREFAALQTELATKEALIDVILVNEVSWSSALL
                     DVSRVIPDASHLTNLTGQIEATVAGEVVAEPTGGTPETTLVGKMTFAGVANQTETIAT
                     WIARLEEVQGWANAWVNSAEEDAPGSRIYTFSNGLDLTQEAVTERGQGVAAP"
     gene            complement(1066..2142)
                     /gene="pilM"
                     /locus_tag="VF195_00725"
     CDS             complement(1066..2142)
                     /gene="pilM"
                     /locus_tag="VF195_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX6579391.1"
                     /translation="MARTRIGVDVGSSAVRVAEVAMGDIPVIVRAAQVPLAPGVVESG
                     EVRQSEAVAEALRKVLAKSGVKSNQVHLGVGNQRVVVREIALPWLPEEELRDTLSFQV
                     QEFIPMASDEVVLDFDPLGELDQGGRRMVRILLVAAHKGMVDALVEAALAAKLDPQDI
                     DLTPFAVIRAIGTDDEGLDLDSSGEEAVIDIGAQVTSICVHDRGVTRFVRMLPFGGRD
                     ITLALASRLGVDDEVAERLKRGDRVQVDGEGATPLPEPAEIRELALTRAESFVDEIRS
                     SLEFYTAQMPNAQIGGVLVVGGGSRLDGLLELLQERLPVPVDRGRLFERAKSEVELSA
                     EASTEAEAVLSVAVGLAIPTRGQA"
     gene            complement(2143..2367)
                     /locus_tag="VF195_00730"
     CDS             complement(2143..2367)
                     /locus_tag="VF195_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013349804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579392.1"
                     /translation="MPKRSHSGWVGRQAGDRLLTVAEVAGTMRVSNMTVYRLIKNGDL
                     AALRVGKNYRIRESDVEMYLSDRAVQVEGA"
     gene            complement(2378..2569)
                     /locus_tag="VF195_00735"
     CDS             complement(2378..2569)
                     /locus_tag="VF195_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016437488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579393.1"
                     /translation="MSKRRPFVGDRLLTVGEVAATMRVSNMTVYRLIKSGQLAAIRVG
                     KNYRIRESDVDRYLSERSV"
     gene            complement(2853..>3117)
                     /locus_tag="VF195_00740"
     CDS             complement(2853..>3117)
                     /locus_tag="VF195_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX6579394.1"
                     /translation="IAPKGMGMGDVKLAGLIGLVLGSIGLGLVGVAAGAGILFGGVGA
                     IVALLLGATRKSRVPYGPFMAAGALLAVFVGASIADAYLSLLN"
BASE COUNT          525 a         1143 c          964 g          485 t
ORIGIN      
        1 gcccgcttca gcgtctcgag taggaacaag aactcgtcga tcgcgaagta ccggccgctc
       61 accgtgatct gcgattcgat cgtggagtag ctcccggtcg ggttcggcac cggggtgccg
      121 ggggtgaacg agaagaagtc caccgacgcc cggtcggccg cgccctggag caagcggaac
      181 acgctcggga ggccggcggt cggcggcacc tgcgtgtcga tcgcggcgat ctccaactcg
      241 gtctccggcg ctgccgcccg agcatcctcg agggccttca gctgggcggc gaggtcgatc
      301 tcctggtcct gcgcctcctg gagccgatcc tcggtctcgc ccacctcatc gatcttcggg
      361 aggatgaaca ggaacacggc gagcagcgcg accagcaaga ccgcacccgc gatgatcggc
      421 gcgcgccggt tcacggtgcc gccactccct ggccgcgttc ggtcacggcc tcctgcgtga
      481 ggtcgagccc gttggagaag gtgtagatcc tcgagcccgg cgcgtcctcc tcggcgctgt
      541 tcacccaggc gttcgcccaa ccctggacct cctcgagacg ggcgatccag gtcgcgatcg
      601 tctcggtctg gttcgcgacg ccggcaaagg tcatcttccc gaccaaggtg gtctcgggtg
      661 tcccgcccgt cggctccgcc accacctctc cggcaacggt cgcctcgatc tggccggtca
      721 gattggtcag gtgggacgcg tcggggatca cccgggacac gtcgaggagc gcgctcgacc
      781 aggagacctc gttcacgagg atcacgtcga tgagcgcctc cttggtcgcg agctcggtct
      841 ggagggcggc gaactcccgg agggacgcga tctgcctcac gagccgcgcg ttggtggact
      901 gctgcgcctc gaggtccgac cggacctgcg acagccgctg gacctggaag aagaagaaga
      961 tcccgatcaa cgcgagcgcg gcgaggccgg gcccgacgac cagcgacgtc gtccggcgga
     1021 cagtctgtcg ctcacggagc tcggggggga gcaggttgac caggctcacg cctgcccccg
     1081 ggtcgggatc gcgagcccga cggcgaccga cagcacggcc tcggcttcgg tcgacgcctc
     1141 cgcagacagc tcgacctcgg acttggcgcg ttcgaacaga cggccacgat ccaccgggac
     1201 cggcagccgc tcctgcaaca gctcgaggag cccgtcgagc cgggaacctc ctccgacgac
     1261 gagcacgcct ccgatctgcg cgttcggcat ctgcgccgtg tagaactcga gagacgaccg
     1321 gatttcgtcc acgaagctct ccgcccgcgt gagcgcgagc tcccggatct cggccggctc
     1381 cgggagaggc gtggcgccct ctccgtccac ctggacccgg tcgccgcgct tgaggcgctc
     1441 ggccacctcg tcatcgaccc cgagtcgcga cgccagggcg agggtgatgt cgcggccccc
     1501 aaagggcagc atgcggacga acctggtcac gccgcgatcg tgcacgcaga tcgacgtgac
     1561 ctgcgccccg atgtcgatga cggcctcctc accgctcgag tccagatcca acccctcgtc
     1621 gtcggtcccg atggccctga tgaccgcgaa cggtgtcaga tcgatgtcct ggggatcgag
     1681 cttggccgcg agggcggcct cgacgagggc atcgaccatc cccttgtgcg ccgcgaccag
     1741 gaggatccgc accatccgcc gcccgccctg gtcaagctcg ccgagcggat cgaagtcgag
     1801 cacgacctcg tccgacgcca tcgggatgaa ctcctggacc tggaacgaca gggtgtccct
     1861 gagctcctcc tcggggagcc agggcagggc gatctcgcga acgacgacgc gctggttccc
     1921 gacgccgaga tgcacctggt tggacttcac gccggacttg gccaagacct tccggagcgc
     1981 ctcggctacg gcctcggact ggcgaacctc gccggactcc acgacgccgg gggccagagg
     2041 cacctgggcg gcccgaacga tcaccgggat atcccccatg gccacctccg cgacgcggac
     2101 agcgctcgag cccacgtcca ccccgatcct ggtcctggcc aactacgcac cctccacctg
     2161 gacggcccga tccgagaggt acatctccac atccgactcc cggatccggt agttcttccc
     2221 gacccgcaac gccgccaggt cgccgttctt gatcagccgg tagacggtca tgttcgatac
     2281 gcgcatggtc cccgccacct cggcgacggt cagcagtcga tcccccgcct gacgacccac
     2341 ccacccgctg tgcgaacgct tcggcatctc gcttcaccta gaccgatcgc tccgacaggt
     2401 accgatcgac gtccgattcc cggatccggt agttcttccc cacgcggatc gccgcgagct
     2461 ggccgctctt gatcaaccgg tagacggtca tgttcgacac gcgcatggtt gcggcaacct
     2521 cgcccaccgt gaggagccgg tcgcccacga acggcctgcg cttgctcatc taacccatct
     2581 ccctcatctg tcccatctgc ctcatgttca acaacttgca cacccgcctc atcccatctc
     2641 atccggtcca gggggctgcg caccgtaaca agtgccacat ctatcgtcaa tcaccccatc
     2701 tgcctacatt cggcacggta ggctaaatca catagatgtt attagctcta tttatcaaaa
     2761 gtcacaccag caacagggcc ccttgcccgt ttaggcgcga ccggggctcg cggatcctcc
     2821 gaggcggagc tcagccgcgg tgaggctcgg gctcagttga ggaggctcag gtaggcatcg
     2881 gcgatcgaag caccgacgaa cacggccagc agggctcccg ccgccatgaa cgggccgtag
     2941 ggaaccctgg acttccgagt ggcccccagg aggagtgcga cgatcgcccc cactcccccg
     3001 aagaggatcc ccgctcccgc cgccacgccg accagcccca gaccgatcga ccccaggacg
     3061 agtccgatca ggccggcgag cttcacgtcg cccatcccca tccccttcgg agcgatc
//
LOCUS       DASUYD010000015        23090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_671449, whole genome shotgun sequence.
ACCESSION   DASUYD010000015 DASUYD010000000
VERSION     DASUYD010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_671449"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VF195_00745"
     CDS             complement(<1..1041)
                     /locus_tag="VF195_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6579395.1"
                     /translation="MIHDEAGRVSAPPFVVMEHITKRFPGVVANEDVSLEVRAGEVHA
                     LIGENGAGKSTLMRVLYGMYPADEGTILVKGDTVKISSPRVAIQHGIGMVHQHFVLVD
                     PFTVTENVILGDEGGAIIDMGAAHEKVGELARIYGFHVLPSAVVEDLSVGEEQRVEIL
                     KALYRGVDLLILDEPTAVLTPIEANELFENLRRLRDDGKAIVFISHKLDEVLDIADRI
                     TVLRRGRVVGETRPSETSKAKLAEMMVGRPVLFRLEKPEVEAGEPVLEVEGLLGEGKL
                     NGISLEVRAREILGIAGVEGNGQRELAEALIGLRPPAAGSIKLAGNDIAGWSVAEIRN
                     AGVAYIPEDRQEQ"
     gene            complement(1066..2187)
                     /locus_tag="VF195_00750"
     CDS             complement(1066..2187)
                     /locus_tag="VF195_00750"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6579396.1"
                     /translation="MRAARGLPAIAFLILLGAFTACSGGGNPCEVPEDKANENGVSVG
                     VAFDIGGIGDKSFNDAAKRGFDEAVDAGLVSEATCIEPNESGSNRDENLIALADDGFD
                     LVFGIGFAFSPAVNENAGNYADQQFAVIDGYATCPPDVCTDIPNPAPPNVTDLTFKEE
                     EGSFLVGVAAAMKAQELGCDTVGFLGGQIGFLIGKFEAGYTEGVHTVDPDITVLVEYI
                     GDSTAAFNDAAAGDQLSTAMYDDGACIIYHAAGASGAGLFTAAVKADKLAIGVDSDQY
                     ELVSAEQKPLVMTSMLKRVDTAVFNAIEEAGNDALEGGTAQVFGIAEDGISYATSNPD
                     VLTQDIVDAIEGYKAQILDGTITPPFCIVKAEMPWCEAA"
     gene            2310..2732
                     /locus_tag="VF195_00755"
     CDS             2310..2732
                     /locus_tag="VF195_00755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6579397.1"
                     /translation="MGEPDTLLASLRDRLATSGFHTWAGMEVVDAAPGEVTVAMRVEE
                     RHVNLQGLVHGGMLAILADTACGLSIRSAMEPGRLHVTADLDIHFLAPAKPGRLVGRG
                     KAITVGRSLAFAEASIEDGEGRLLAKAQSRFSVRGTET"
     gene            complement(2729..3112)
                     /locus_tag="VF195_00760"
     CDS             complement(2729..3112)
                     /locus_tag="VF195_00760"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX6579398.1"
                     /translation="MSVRFLSDEWAQALKAELNESEEFQRAAAGQHATIQQVITGGDG
                     DTHYWITIEDGRIDLGVGDVEGEDATITQSYDSAAGLARGELSPVTAFMTGKLKIAGN
                     MGLILGLQGALAQLPAAMAKIDVQY"
     gene            complement(3183..3830)
                     /locus_tag="VF195_00765"
     CDS             complement(3183..3830)
                     /locus_tag="VF195_00765"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6579399.1"
                     /translation="MTDRVLYLIRHGQSDFGWQGEPWASARGEQWDPPLSERGAEQSA
                     LLAGRLLVMDLEPFVVYSSPLRRANETAEAFARLAGTDVTVDDELVEAHIGGWEGKPF
                     EEIIGSDPDVVQHIRNQRAIWHRAPGAEGGDGFRTRVRTAVDGIVDGHPGENVLVFAH
                     GGVINAYVGDLLGLRQEMFFLPENTSLNSVDVDGETRTVRFLNDVLHLTDPLFFE"
     gene            complement(3827..4222)
                     /locus_tag="VF195_00770"
     CDS             complement(3827..4222)
                     /locus_tag="VF195_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579400.1"
                     /translation="MAVGEPARARMWETGGRWFAHILDVSAYIEVTATSRAGCLEELR
                     KITGDDVDLMVEVIPAVVGVAEAAEIMGWDKRRVITYIDRGSFPEPLTTLASGRIWIR
                     EDIEGYADHWRATHPPPPKSRSTTLSAPS"
     gene            complement(4445..4732)
                     /locus_tag="VF195_00775"
     CDS             complement(4445..4732)
                     /locus_tag="VF195_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579401.1"
                     /translation="MPRKNRRDPGYFQPQEAPRPRSAAPRWAELAGYDVRHVGGEKEY
                     RCPGCDHIVRAGIWHLVVIPLDAPDERRHWHTECWRKELRRIGAYRPPPPE"
     gene            complement(4732..5400)
                     /locus_tag="VF195_00780"
     CDS             complement(4732..5400)
                     /locus_tag="VF195_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEX6579402.1"
                     /translation="MAGPEESLRIDTVKGPVSGKYTRPRTPFATIVVAHGAGAGMDQP
                     FLVGFARACQEEGLATLRFNFPYVESGRRAPDAEGVLRDAWRAAFDAASARRKAEPVW
                     VSGKSLGGRIASMAAADREIDPAGLVFLGYPLHPPGKPERIRDEHLYRIEAPMLFLQG
                     TSDPFASPELLRSVVERLGDLATLVPFEGGGHSFEVRGRKRDPREVGASLAPHAAAFI
                     RDHD"
     gene            5421..6158
                     /locus_tag="VF195_00785"
     CDS             5421..6158
                     /locus_tag="VF195_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6579403.1"
                     /translation="MAGTLHLGTSGFSYDGWKHGVFYPEGLKNREMLSYYSQQLSSVE
                     INYTFRRFPTEKSLTAWREQAADGFTFTLKANQRITHFKRLADTDEDVRMFLDLAQLL
                     GDKLGCVLFQCPPSLHYDRALIEAFVGYLPPGGRFAMEFRHASWIEAKDLLLSQGVAW
                     CVAETDEKDPSPDDLSWEPAGYLRLRKTEYSDEELRGWADRIGPALEAGADVFCYFKH
                     EEDAAGPKMAKRLRETLEDRAPDKASA"
     gene            complement(6218..8680)
                     /locus_tag="VF195_00790"
     CDS             complement(6218..8680)
                     /locus_tag="VF195_00790"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6579404.1"
                     /translation="MGAAPEPVATLRAEVLALERAYSPGHHGVWAARRRAELVDAAIV
                     ALFARAGAPSGTAIAAVGGYGRGLLLPRSDIDLLVLHDGSDPGAVARLADALLYPLWN
                     DGLDVGHAVRTPQECGSAAGRLDVGAAMLDIRFLSGDGGLVADVAGRIAEWARRDPRA
                     FARALRDDAARRAERFGSTAHLLEPDLKEGAGGWRDLHSIWMLESAVGMPLEEAGLLR
                     GREREALAAAEEFLVRVRSALHLESGKRGERLVLDHQPAIARAMGFEDEPRLIAIDGL
                     MRSLFEHARDVEFLFRAVTERLREAGASPITPPTTPAGVLEALAAEAEADREPTAALL
                     DSVDDLGIRDEVVWDADVRAAFSRLLRAGDRGAASLEALDRLGILAMFLPGWADVRCR
                     PQRDPYHHLTVDAHLTGSLRAMGRLLAGEGACADPVQAEAVGQVSDRDPLLLGALLHD
                     IGKNGEGGHVPVGARVVASLLDRMGVEPPARDLVRFLVERHLLLPDTSTRRDLSDENL
                     VLDVAAVVGSPDRLAALYLLAKADAEATGPAAWTSWRRTLIHELVAKVEHVLERGEMG
                     TEIAERLAERIDRIGSILDDEPREKVERFLRRMPRAYLLAADLGRVARHYATIAPPVG
                     TKDVRAVHFDGTRPGTYEVLVVARDRAGLLSWIAGSLALAGMSILTAHVFTTDDGVAV
                     DLFEVEGVWEPEVPERRWREFRGVLRRAVDGSISLERRVDEKRRWYPPPRVPTPVTLT
                     VDNDASDFSTVIEVGAPDRLGLLYDITRTFAELGIDVHLAKVATYEGRVIDSFYVRDT
                     LGAKLSDDLDTLEKSLRHRLES"
     gene            complement(8688..9026)
                     /locus_tag="VF195_00795"
     CDS             complement(8688..9026)
                     /locus_tag="VF195_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018850023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX6579405.1"
                     /translation="MKLVTAIVKPHRLDEVKDALREIGVNGLTTTDVEGFGRQRGHTE
                     VYRGAEYQVDFVPKVRVEVVVDDTEAQGVVDAIVKAARTGKIGDGKLWVTEVEQLVRI
                     RTGEMGPDAL"
     gene            complement(9037..9975)
                     /locus_tag="VF195_00800"
     CDS             complement(9037..9975)
                     /locus_tag="VF195_00800"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6579406.1"
                     /translation="MILPPAGFTVRPATRDDIDRMAGLAQAVDRSDDGVVEPVRGHIE
                     DEWANPLFRAGEDTMLAFAQDGSLAACATCWGIDPASSVEAWINVHPDHRGEGLGTWF
                     VGWAEGRARRSIRETGDSTLLRPIVSSSGDGRAFLERLRYRHVRTFWHMERRLDGTES
                     AGRPPDGVTIRPFQDGDGPALHRVLEASFEGHFGSEPMAYEAWEEANLRAPSTELSLL
                     FVAERGGELVGALTTAIDEQAWVAELGVLEAHRGVGIGRALLRRAFSDLAVRGHTIVK
                     LNVDGENASGATLLYEGVGMTRGRSWLFYEKRIDAD"
     gene            complement(9972..10859)
                     /locus_tag="VF195_00805"
     CDS             complement(9972..10859)
                     /locus_tag="VF195_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6579407.1"
                     /translation="MWKGAISFGLVTIPVSVYPATEEKTLRFNQLHDDDGGRIRMKRV
                     CSVDGEEVGYEHIVKGYEYEKDRYVILTDDDFEAIPVESSRAIDIQQFVDLEEIDPML
                     YKKSYYLVPEETGAKAYALLREALNRSGKVGVAKVSFRDKEHLAALRFRDDAFILETM
                     YWPDEIREADFGGVDVSMKVRPNELDMAQTLIDNLSADWDPGEFKDEYREAMLRIVEA
                     KINGEEIEVVEAEPTAKVVDLMEALKASVAAAKKEAKAEPATKKKAPARKKPAAKKPA
                     AKKPGAKKPAAARKKAVGE"
     gene            11035..13590
                     /gene="ligD"
                     /locus_tag="VF195_00810"
     CDS             11035..13590
                     /gene="ligD"
                     /locus_tag="VF195_00810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX6579408.1"
                     /translation="MPPRRRTTSSGPAFTLDLPRALEARKDGDAWWVEVDGRDLRLSN
                     LNKVFWPDEGYTKGDLLAYYWNIADLIGPHLEGRPLTMKRMPDGAEGPHFYEKTAPSH
                     TPDWVHRCQVRSDDSKRGRIDYLMVDDTATLLYVANLGCIEMHPLHSRCEDVAHPDYL
                     FFDLDPFEPYTYEDVLAVARHIKVVLDQLGLTAYPKTSGATGLQIYLPVERGTYTYGQ
                     VRAFVGACGRMITKADPDRVTMAWKIADRTGKVFIDHNMNRQGANIAAAYSLRPEHRA
                     PVSTPLTWDEVFEGGFEPQDFRIDNVWDRFAERGDLFAGVRTEAMDLSTAFDALDVRP
                     EDDGPGFSSALPRTAAPSVVRKTSEEIVEESRDPNLREYVRRRDFGEAGTTEPAPGEV
                     AGTGNSFVIHKHRATRLHYDVRLEREGGLPSWAVPRGLPTTRGDKRLAVETEVHPLEY
                     GAFEGTIPEGHYGAGEVRIFDDGWYEPIEWTDSKVSFRLHGRRYPGLEFHFVKTRTDW
                     LAFLASAQDAPPVATPPRYQPMLAEGGGEPFDDPRWRFEPKLDGIRALAEMETGGTVF
                     RTRRGRDVTFQYPDIHMIHELVDQVNAVLDGEIVAVEDGGRTSFERLQQRMNLQNERE
                     IKRVAKQIPVSFVVFDLLWLDGHDVTGLALEERRELLDLIVEEDHRLQLMSYEEGDGT
                     AFVDGARALGLEGVVAKRTGSKYLPGRRSPDWKKIKLISTQDCVVLGWTLGQGGRAGS
                     FGALLVGAIDDGAMRWVGQVGTGFNHQMLDRLMEQLTPLVREDPPIDDPSLRKVKGAR
                     FVEPELVCEVEYLEITKSSGKMRAPSFRGLRPDKTPDECVLEPPAFDKVRRKR"
     gene            complement(13600..14103)
                     /locus_tag="VF195_00815"
     CDS             complement(13600..14103)
                     /locus_tag="VF195_00815"
                     /inference="COORDINATES: protein motif:HMM:NF036411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5319 family protein"
                     /protein_id="HEX6579409.1"
                     /translation="MDDERDEPYELTAEERRDIQSDLDDLSSMRAVFASQSVKGVVIA
                     CQECGANHFYEWELLRDNLEHMLQSGEPRMHEPAFDIVEEEYIQWDYGKGYVDALTDT
                     GLEPDRRIELTRCPWCQVPFAEDHAFCPRCGRSMGAVRLYQELVGKGMEERDVRAMLV
                     RAGFEPF"
     gene            complement(14103..14807)
                     /locus_tag="VF195_00820"
     CDS             complement(14103..14807)
                     /locus_tag="VF195_00820"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6579410.1"
                     /translation="MTFSRAMVLFAHPDDAEFFCGGTVARWSREGCDVHYVCATDGSA
                     GSNESGTTREAMREVREREMRAAADILGVASVTFLGEVDGFLAVTPDTRRKVTREVRR
                     LRPDVLVAPDPSRLWSGSGYINHADHKAAGELALCAVMPDAPTRVMFQELEDEGLEPF
                     EVPNLYLSAEEADTFVDITDALAVKLKALSAHASQLGPDVEERVIARAREVGERAGCE
                     FAEGFRGFRLTDDEDD"
     gene            14890..16365
                     /locus_tag="VF195_00825"
     CDS             14890..16365
                     /locus_tag="VF195_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579411.1"
                     /translation="MLTERLRATSIVWRFAVTSFVVFGLIGVGIGALRAEDLRTRSEE
                     AATVRAELIAESVIAPLLVPEDLQGPIRGVRYHVLDRAIHEFAMNDAGVERVKVWNVD
                     GMVVFSNDPEQVGLRPAMEEDLLEAFEGEVASEISDLSEAENVSERALADQLFETYVP
                     VHLPGDGESNEATAVIEVYQDYSAIQLEIDHLNDTLKISLGIGLLVLYVLLLPVMVGT
                     TRTLRRQNAQLSEQADQLGVLLARERETVAELRELDRLKSDFAAAASHELRTPLTTIR
                     GYAELLRDRGPADDPSTRDAIDAIVRQTGHLQRLVGNLLREAQLEHGSHETREEPTSI
                     ADVLGGVRRGFPGSVGRIHVRTEPDIPSMLLDPVTLHEIVANLVDNALKYSTPGAPVE
                     VTAEVEGNALVIRVRDRGEGIPPGDLPRIFDRFTQIDGSSTRAHGGVGLGLHLVRELS
                     RRSGGEVEVDSVMGMGTTFTVTLPISTAGQPDPQRPEMTTA"
     gene            16409..17500
                     /locus_tag="VF195_00830"
     CDS             16409..17500
                     /locus_tag="VF195_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579412.1"
                     /translation="MADGDRISELERSFRRAGLPTFVRGYSARRAFAKALPLLAIVFV
                     LEILNALNFDFGFWTNVGFLAGGIAISLGMIAILNLARGQRFLSIPRLVGAPEMIVFV
                     LVPSVLPFLFGKQRTSAIVTLAGNTALLGLVYLVLGFGAVSILEWAVRRFMSLFAASL
                     TVLVRALSLLLFFLLVIFFTTETWQIWTVPGLPKFFAAAGLFVLLAAGSLLLRLPGSV
                     RGLEAELQGERLSRTQRVNVGLVMFLSQFLQVVFVAFAVWLFFLVFGSLLVSAGVREA
                     WLGTLGTEVVRIPFFGDTVVVITQELLRVATGMASFAALYYAAATQLDEAYRDEVVER
                     IAEQMKETFARRAEYLRLVGGSPAHETVG"
     gene            17705..17905
                     /locus_tag="VF195_00835"
     CDS             17705..17905
                     /locus_tag="VF195_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX6579413.1"
                     /translation="MATGTVKWFSPEKGFGFITQSEGPDVFVHFSGITGEGYKNLEEG
                     QAVEFDIVDGQKGPQAQNVRIT"
     gene            complement(17972..18310)
                     /locus_tag="VF195_00840"
     CDS             complement(17972..18310)
                     /locus_tag="VF195_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579414.1"
                     /translation="MEFGDALERFGFRLREERGFGRGAQLYVAQPTPYLTYTVHAYPD
                     GTAIFSWEFALGEYLLTKGIQVGSDETLNQFAYPREELRGSQDGTWLTSAVERTEGLL
                     ADLRFDRPRD"
     gene            complement(18335..19384)
                     /gene="rmuC"
                     /locus_tag="VF195_00845"
     CDS             complement(18335..19384)
                     /gene="rmuC"
                     /locus_tag="VF195_00845"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEX6579415.1"
                     /translation="MTNDVVIAVLAAFVGIAVGALMMLRRQPTQQQDPDSVSTLDAQT
                     ALNAQLHAELTARLEVQAAELRRIADASGQRDVSADHLRAGLEATRHALEQLQVRDEE
                     RRTSETENREVVRRLATVLAGGNTKGRAGEHVLREHLGQLPPSMLASDFRVNGKVVEF
                     GLRLPDGRRLPIDSKWSAVAELEAMEAEEDPTRRDACARKVEKAVAARAKEVGQYIDA
                     SVTAPVAVAAVPDAAYQVLSRAHTEAFMRGVVIVPYSSALPIIMFLYALVERFGDAAD
                     VQAALAEVGTLLDAMDAIVENKFSKAGTMIANGADEFRSNLGKARGSIARARHAEQLQ
                     LMDAQSEDEVLSIVR"
     gene            19490..20569
                     /locus_tag="VF195_00850"
     CDS             19490..20569
                     /locus_tag="VF195_00850"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX6579416.1"
                     /translation="MTPHPIELYRLRGTHHKVGIQMGELGADQIHRALGSFDADLPAG
                     RGRTEQLRLAQAFRDVTAPSLPWLIEELDGCAEGAGVDPLEFFATTVEELWYSPYPKR
                     TQGRCSDVVAGPAATADGHLWVGHNNDLRPEAEADIVAIEKAVEDEPVVLQIGGVPWL
                     SVGWNQAGLSLTGNELSPNDERVGISRSHQVFEMLRAHNLHEMVAYAIRPDRASSYNN
                     VLADRFGDVANVEGSATAVEITHLDERDHLAHTNHYVSERMQPYEGDPGYAVRSDVRF
                     CRARDLLADRPPGSVTPEVLREILSDHENQPNEVCRHPEWGHPTSKTVFWCIADVTEG
                     RIAYGRGNPCDSMEQEYAFAGYGEG"
     gene            complement(20554..21228)
                     /locus_tag="VF195_00855"
     CDS             complement(20554..21228)
                     /locus_tag="VF195_00855"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX6579417.1"
                     /translation="MASTSDDLFAAIEAGDLGGVETILDADPAAGDMRDETGVSALMR
                     ALYRFDTDLVEIVRAHAGAPDVFEAAALGEVDRLRAVLDSEPTAATTYSGDGFTALHF
                     AAFFGQPEAAALLIERGAEVDAFGRGWMTGTALHSAASRLQSDVVRILVEAGANPDVR
                     QSAGWTPLHAAAMNGDLPSMELLLRAGADPAATNDEGRTVADLAGESGDDATIERVRS
                     ALQPSP"
     gene            21284..21745
                     /locus_tag="VF195_00860"
     CDS             21284..21745
                     /locus_tag="VF195_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6579418.1"
                     /translation="MTTSRRTSPTREVGEVGRVADRLHSATIHLLRRLRVEDEALGIS
                     APRLSVLSVLVFAGPKRIGKLARIEQVEPPTMTKLVDGLVRDGLALREADPDDARAVR
                     VRATPTGARTLRRGRKRRLETLQRTLETLSPRDLAALGEGVDVLQRVLGDR"
     gene            complement(21742..22926)
                     /locus_tag="VF195_00865"
     CDS             complement(21742..22926)
                     /locus_tag="VF195_00865"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6579419.1"
                     /translation="MAKSIYVANVTVFDGNKVKKRQGVLFGADGIEWVGAHPRPPKDA
                     RDARVVEGDGKTLLPGLIDVHVHLQFDGEADFEKEARDLTTPGFAALKAMVNAKRNLD
                     AGVTTVRDLGGMGGASIDVARAVAAGLVPGPRILAAGRALTVTGGHGHNIAFSREVDG
                     PDAVRAAVREEIRAGAAAIKLIATGGVLTPGIPATFSAFTAEELEAGVREAHERNLSV
                     AAHAIGANGIRAAVLAGVDSIEHCNQLTTSTAREMESRGTFRSPTICAVRGILDHADR
                     VAAYAVEKARAIEEDSKSSLRLAVRSGVRPVCGTDAGTPFNAHGNAAQEVVYMAEWGM
                     APLDALRAATANGAELLRLADVGTIEVGKRADLVLVDGDPVEDPRALLGRKRVWRAGD
                     PV"
BASE COUNT         3542 a         8185 c         7708 g         3655 t
ORIGIN      
        1 ctgttcctgt cgatcctccg ggatgtaggc cacgcccgcg ttccggatct ccgccaccga
       61 ccacccggcg atatcgttgc cggcgagctt gatggagccg gcggccggcg ggcgcaggcc
      121 gatcaacgcc tcggcgagct cccgctggcc gttgccctcc acgccggcga tcccgaggat
      181 ctcacgggct cgcacctcga gggagatgcc gttcagcttc ccctcgccca gcagcccctc
      241 cacctcgagc accggttccc cggcctcgac ctcgggcttc tccagacgga acagcacggg
      301 ccgcccgacc atcatctcgg cgagcttcgc cttcgacgtt tcggacggcc tcgtctcgcc
      361 gacgacccgt ccacgcctga gtacggtgat gcggtcggcg atgtccagga cctcatccag
      421 cttgtggctg atgaacacga tggccttgcc gtcgtcgcgg aggcgccgca ggttctcgaa
      481 gagctcattg gcttcgatcg gcgtcagcac cgccgtgggc tcgtcgagga tcaggagatc
      541 gactccccgg tagagggcct tgaggatctc gacccgctgc tcctcgccga cggacagatc
      601 ctccaccacc gcgctcggga ggacgtggaa accgtagatg cgcgccagct cgccgacctt
      661 ctcatgcgcg gcacccatgt cgatgatcgc gccgccctcg tcaccgagga tcacgttctc
      721 cgtcaccgtg aacgggtcca cgagcacgaa gtgctggtgc accatgccga tcccatgctg
      781 gatcgctacg cgcggcgagg agatcttcac cgtgtcgccc ttgacgagga tcgttccctc
      841 gtccgcgggg tacatcccgt agaggacccg catcagggtc gatttcccgg cgccgttctc
      901 gccgatcagg gcatggacct cgcccgcccg cacctcgagc gagacgtcct cgttcgccac
      961 gacgcccggg aaccgcttgg tgatgtgctc catcacgacg aaaggcgggg cgcttacgcg
     1021 ccccgcctcg tcgtggatca tcggttcacc gtcgccggca cgtggctagg cggcctcgca
     1081 ccatggcatc tcagccttga cgatgcagaa cggcggcgtg atcgtcccgt cgaggatctg
     1141 cgccttgtat ccctctatcg cgtcgacgat gtcctgcgtc aacacgtcgg ggttgctcgt
     1201 cgcgtacgag atgccgtcct cggcgatccc gaacacctga gccgtcccgc cctccagggc
     1261 gtcgttcccg gcctcctcga tcgcgttgaa aaccgccgtg tccacgcgct tgagcatcga
     1321 ggtcatcacg agcggcttct gctcggcgct gaccagctcg tactggtcgg agtcgacgcc
     1381 gatcgcgagc ttgtcggcct tgacggccgc cgtgaacaac cccgccccgg atgcaccggc
     1441 cgcgtggtag atgatgcagg cgccgtcgtc gtacatcgcg gtcgagagct ggtctcccgc
     1501 ggccgcgtcg ttgaacgcgg cggtgctgtc cccgatgtac tcgaccagca ccgtgatatc
     1561 gggatcgacg gtgtggacgc cttccgtgta gccggcctcg aacttcccga tcaggaagcc
     1621 gatctgcccg cccaggaagc cgacggtgtc acagcccagc tcctgagcct tcatcgccgc
     1681 ggcgacgccc accaggaacg aaccctcttc ctccttgaac gtcaggtcgg tcacgttcgg
     1741 cggagccggg ttcgggatgt ccgtgcagac gtcgggtgga cacgtggcgt atccgtcgat
     1801 cacggcgaac tgctggtcgg cgtagttccc ggcgttctcg ttcaccgccg gcgagaacgc
     1861 gaacccgatc ccgaacacga gatcgaagcc gtcgtcggca agcgcgatca ggttctcgtc
     1921 acggttcgat ccggactcgt tcggctcgat gcaggtggcc tcgctgacca gtcctgcatc
     1981 gacggcttca tcgaaccctc gcttggcggc gtcgttgaag gacttgtccc cgatgccacc
     2041 gatgtcgaac gcgacgccga cagagacgcc gttctcgttg gctttgtcct caggaacctc
     2101 gcacgggttc ccgcccccgc tgcacgcggt gaacgcaccg agcaggatca ggaacgcgat
     2161 cgcgggcagc cctcgggctg ctctcatact ctcccccctc tctcgggacg gccttggccg
     2221 ccggcaaggg attctccggg cggacgcagc ttccgtcaaa cccctgctgc tgggcccgtg
     2281 ggagactacg agaagggagg cccgaaggga tgggggaacc cgacacactg ctggcgtcgc
     2341 ttcgagatcg actcgccacc tcgggcttcc acacgtgggc gggcatggag gtcgtcgacg
     2401 ccgctcccgg cgaggtgacg gtggcgatgc gtgtggagga acgccacgtg aacctccagg
     2461 gcctcgtcca cgggggcatg ctggcgatcc tcgccgacac cgcgtgcggc ctgtcgatcc
     2521 gatcggccat ggaacccggc cggctccacg tgaccgcgga cctggacatc catttcctcg
     2581 ctccggcgaa gccgggcagg ctcgtcggac gggggaaggc gatcacggtg ggacgctcgc
     2641 tggccttcgc ggaggcgtcg atcgaggacg gtgagggccg gctgctggcg aaagcccagt
     2701 cgcgcttctc cgtccgcggg accgagacct agtactgcac gtcgatcttc gccatagccg
     2761 cgggcagctg cgccagggct ccctgcagac cgaggatcag acccatgttg ccggcgatct
     2821 tgagcttgcc cgtcatgaac gcggtgacgg gcgagagctc tccgcgggcc agccccgccg
     2881 ccgagtcgta gctctgcgtg atcgtggcgt cctcaccctc gacatcaccc acaccgaggt
     2941 cgatgcggcc gtcctcgatc gtgatccagt agtgcgtgtc gccgtcgccg ccggtgatga
     3001 cctgctggat cgtcgcgtgc tggcccgcgg ccgcccgttg gaactcttcg ctctcgttga
     3061 gctccgcctt gagcgcctgc gcccattcgt ctgacaggaa cctcacggac acgatgaccc
     3121 cctcggatgt cgatgactgg agtggatgat gcggtggagg tcccgggagt ataggagggc
     3181 cctcactcga agaagagcgg atcggtgaga tgcagcacat cgttcaggaa gcgcacggtg
     3241 cgcgtctcgc cgtcgacgtc gacgctgttc aggctcgtgt tctcggggag gaagaacatc
     3301 tcctgccgca gcccgaggag gtccccgaca taggcattga tcacgcctcc gtgggcgaag
     3361 accagcacgt tctccccggg gtgtccgtcg acgatcccgt cgaccgcggt tcgcacccgg
     3421 gtgcggaacc cgtccccgcc ctccgcacca ggcgcacggt gccagatcgc gcgttggttc
     3481 cggatgtgct gcacgacgtc gggatcggag ccgatgatct cctcgaacgg cttgccctcc
     3541 catccgccga tgtgcgcctc cacgagctcg tcatcgacgg tgacgtcggt tcctgccagc
     3601 cgcgcgaacg cctccgcggt ctcgttcgcg cgtcggagcg gggacgagta gacgacgaac
     3661 ggttcgagat ccatcacgag gagccggccc gcgagcaggg cggactgctc ggcaccgcgc
     3721 tccgacagcg gcggatccca ttgctcgccg cgggcgctcg cccatggctc gccctgccag
     3781 ccgaaatcgg actgaccgtg ccggatcagg tagaggacgc ggtcggtcac gagggcgcgg
     3841 agagcgtcgt cgaacggctc ttcgggggcg gaggatgggt cgcccgccag tgatcggcgt
     3901 acccctcgat gtcctcacgg atccagatcc ggccggacgc gagcgtggtg aggggctccg
     3961 ggaacgaccc ccggtcgatg taggtgatga cccggcgctt gtcccagccc atgatctcgg
     4021 cggcctccgc gacccccacc accgccggga tcacctccac catcagatcg acgtcgtcgc
     4081 ccgtgatctt ccggagctcc tcgagacatc cggctcgact cgtcgcggtg acctcgatgt
     4141 acgcggagac gtcgaggatg tgggcgaacc atctccctcc ggtctcccac attcgggccc
     4201 gggcaggttc gccaaccgcc atgtcgggga tgctaacgcg gccgttcggc gacgtcgccg
     4261 gtccgtctac cacccgcagc agggcaggag gtagccgcac gatgggcagc ggtagtgcgc
     4321 gtgctcgggc tccatcggtg acccgcaggc atcgcacggc agacgcttcg actctgcagg
     4381 aggcagacag cgatcggtct cggctggggt gtccatcctc gacacgatag cccgccgacc
     4441 gacgtcactc gggaggcgga ggccgatacg cgccgatccg ccgcaactct ttccgccagc
     4501 actccgtgtg ccagtgacgg cgctcgtcgg gcgcgtcgag cgggatgacc acgaggtgcc
     4561 agatgcccgc ccgcacgatg tggtcgcatc cggggcatcg atactccttc tcgccgccga
     4621 catgtcggac gtcgtagccg gccagctcgg cccaccgggg cgcggccgac cgcggccggg
     4681 gggcctcctg cggttggaag tatccgggat cgcggcggtt cttccgcggc atcagtcgtg
     4741 gtcccggatg aacgccgcgg cgtgcggggc cagggacgca ccgacctcgc gaggatctcg
     4801 cttccggccg cgaacctcga acgaatgccc cccaccctcg aacgggacga gcgtggcgag
     4861 atcgcccagc ctttccacga cgctccggag gagctcgggt gaggcgaacg ggtccgaggt
     4921 cccttggagg aagagcatcg gggcctcgat ccggtacagg tgctcgtccc ggatgcgctc
     4981 cggcttcccc ggcgggtgca ggggataccc gaggaagacg aggcctgccg ggtcgatctc
     5041 ccggtcggcc gcggccatcg aggcgatccg gccaccgagc gacttgccgc tcacccacac
     5101 gggctcggcc ttccgacgcg cggaagcggc atcgaacgcc gcacgccagg cgtctcgcag
     5161 gacgccctcg gcgtccggcg cgcgcctgcc gctctcgacg tacgggaagt tgaaccgcag
     5221 cgtggcgagc ccctcctcct gacacgcccg ggcgaacccg accaggaagg gctggtccat
     5281 cccggcgccg gcgccgtgcg cgaccacgat cgtcgcgaac ggcgtccggg gccgtgtgta
     5341 cttcccggac accggtccct tcaccgtgtc gatgcgcagc gactcctccg gaccggccat
     5401 cgcgtgccat gatgcacgcg atggccggca ccctccacct gggcacttcg ggcttctcct
     5461 acgacggctg gaagcacggc gtcttctatc ccgagggact gaagaaccgc gagatgctct
     5521 cgtattactc gcaacagctg tcgtcggtcg agatcaacta cacgttccgc cggttcccca
     5581 cggagaagtc gctcaccgcc tggcgcgagc aggccgccga tggcttcacg ttcaccctga
     5641 aggcgaacca gcggatcacg cacttcaaac gactcgccga cacggacgag gacgtccgca
     5701 tgttcctcga cctcgcgcag ctcctcggtg acaagctcgg gtgcgtgctc ttccagtgcc
     5761 ctccctcgct gcactacgac cgagcgctga tcgaggcgtt cgtcggctac ctccccccgg
     5821 gaggacggtt cgcgatggag ttccggcatg cgtcgtggat cgaggcgaag gacctcctgt
     5881 tgtcccaggg cgtggcctgg tgcgtggccg agaccgacga gaaggacccg agtcccgacg
     5941 acctctcgtg ggagccggcc ggctacctcc ggctgcggaa gaccgagtac tccgacgagg
     6001 agctccgggg ttgggcggac cggatcggcc cggcgctcga ggcgggcgcc gacgtcttct
     6061 gctacttcaa gcacgaggag gatgccgcgg gcccgaagat ggcgaagcgg ctgcgcgaga
     6121 cgttggagga ccgggctcca gacaaggcgt ctgcctgaca cacccgtcgt tcgggcgacc
     6181 cgtcccggga tcctcgcgtg actccccccg aaccgtgtca ggactccaag cgatggcgca
     6241 gtgacttctc gagcgtgtcg agatcgtccg agagcttggc accgagcgtg tcccggacgt
     6301 agaaggagtc gatgaccctt ccttcgtagg tcgccacttt cgcgaggtgc acgtcgatgc
     6361 cgagctcggc gaaggtcctg gtgatgtcgt agaggaggcc gagccggtcc ggggcgccga
     6421 cctcgatcac ggtcgagaag tccgaggcgt cgttgtccac ggtgagtgtg acgggggtcg
     6481 gcaccctggg cggcgggtac cagcgccgtt tctcgtccac cctccgttcg agcgagatcg
     6541 agccgtcgac cgcgcgacgg agcaccccgc ggaactcgcg ccagcggcgc tcgggtacct
     6601 cgggctccca caccccttcc acctcgaaga ggtcgaccgc gaccccgtcg tcggtcgtga
     6661 acacgtgggc cgtgagtatc gacatgcccg cgagggcgag ggaccccgcg atccacgaca
     6721 ggagtcctgc gcgatccctg gcgacgacga gcacctcgta cgtgccgggt cgcgtcccat
     6781 cgaagtggac ggctcggacg tccttggtac ccacgggcgg cgcgatcgtc gcgtagtgcc
     6841 gagcgaccct gcccaggtcg gcggcgagga ggtaggcgcg cggcatccgc cggaggaagc
     6901 gctcgacctt ctccctcggc tcgtcgtcga ggatgctgcc gatccggtcg atcctctcgg
     6961 cgagccgttc cgcgatctcg gtccccatct cgccgcgctc caagacatgc tcgaccttcg
     7021 ccacgagctc gtggatgagc gtccgtctcc atgacgtcca cgcggcggga ccggtcgcct
     7081 cggcatcggc cttggcgagc agatagagcg cggcgaggcg atcgggggaa cccaccaccg
     7141 ctgcgacgtc gagcacgagg ttctcgtcgg acagatcccg gcgggtcgag gtgtccggca
     7201 ggagcaggtg ccgttcgacc aggaagcgga cgaggtcgcg ggccgggggt tcgacaccca
     7261 tccggtcgag gagcgatgcg acgacccgcg cgccgaccgg cacgtgcccc ccctccccgt
     7321 tcttgccgat gtcgtgcagg agcgctccga ggagcagcgg atcgcggtcg gagacttgcc
     7381 cgacggcctc cgcctgcacc ggatcggcgc atgccccctc gcccgcgagg agccggccca
     7441 tcgcgcggag cgacccagtg agatgggcgt ccaccgtgag gtggtggtag ggatcgcgct
     7501 gcggacgaca tcgcacgtcg gcccatcccg ggaggaacat ggcgaggatc cccaaccggt
     7561 cgagggcctc gagcgacgcc gcgcctctgt cgcccgcccg gaggagtcgc gagaaggcgg
     7621 ctcgcacgtc cgcgtcccac acgacctcgt cgcggatgcc gaggtcgtcg accgaatcca
     7681 gcagggccgc cgtcggctcc ctatcggcct ccgcctcggc ggcgagggcc tccagcacac
     7741 cggccggcgt cgtcggcggg gtgatcggcg atgcgcccgc ctcgcggagc cgttcggtca
     7801 ccgcacggaa caagaactcg acgtcccgcg cgtgctcgaa cagggaccgc atcagcccgt
     7861 cgatcgcgat gagccgaggc tcgtcctcga accccatcgc ccgcgcgatc gcgggctggt
     7921 gatcgaggac cagccgctcc ccccgcttcc cggactcgag gtgtagcgcg ctccgcacac
     7981 gcacgaggaa ctcctccgcg gccgcgagcg cttcgcgctc gcggccccgg aggagcccgg
     8041 cctcctccag cggcatcccg accgccgact cgagcatcca gatcgagtgg aggtcgcgcc
     8101 atccgccggc accctccttg aggtcgggct cgagcagatg cgcggtcgag ccgaaccgct
     8161 cggcgcggcg tgccgcgtcg tcgcggagcg cccgcgcgaa cgcgcgcggg tcccgccgag
     8221 cccactccgc gatcctccca gcgacgtccg ccacgagacc cccgtcgccc gagaggaagc
     8281 ggatgtccaa catcgcggcg cccacgtcca gccggccggc agctgatcca cactcctggg
     8341 gcgtgcgtac cgcatgaccc acgtcgagtc cgtcgttcca gagcgggtac agcagggcgt
     8401 cggcgagccg cgccacggcg cccggatcgg aaccgtcgtg gaggacgagc aggtcgatgt
     8461 cggaccgggg aaggagcaac cctcgcccgt acccccccac cgccgcgatc gccgtcccgg
     8521 atggcgcgcc ggcgcgcgcg aacagcgcga cgatcgccgc gtccacgagc tccgcccgcc
     8581 ggcgcgccgc ccacaccccg tggtgcccgg gcgagtacgc ccgctcgagc gcaaggacct
     8641 cggcccggag ggtggcgacc ggttcgggcg ctgcgcccat cggagtgcta gagggcgtcg
     8701 gggcccatct cgcccgttcg gatccggacg agctgctcca cctcggtgac ccagagcttg
     8761 ccgtccccga tcttgccggt ccgggcggcc ttgacgatcg cgtcgacgac gccctgcgcc
     8821 tcggtgtcgt ccacgacgac ctcgacgcga accttcggga cgaagtcgac ctgatattcc
     8881 gctcctcggt acacctcggt atgcccgcgt tgccgcccga acccctcgac gtcggtggtg
     8941 gtgagcccgt tcacgcctat ctcgcgcaga gcgtccttca cctcgtccag ccggtggggc
     9001 ttcacgatcg cggtgacgag cttcatgcgc cgcatcctag tcggcgtcga tccgcttctc
     9061 gtagaagagc cacgaccgtc cccgtgtcat cccgacgccc tcatagagga gagtggcccc
     9121 ggaggcgttc tcgccgtcca cgttgagctt gacgatcgtg tggccccgca cggccaggtc
     9181 ggagaaggcc ctccgaagga gtgcccggcc gatcccgacc ccgcgatggg cctcgagcac
     9241 gcccagctcg gccacccacg cctgctcgtc gatcgccgtc gtgagcgcgc cgaccagttc
     9301 ccctccccgc tcggctacga acaggagcga gagctccgtc gagggcgccc tgaggttcgc
     9361 ctcctcccac gcctcgtaag ccatcggctc ggagccgaag tgaccctcga aggatgcctc
     9421 gaggacgcga tggagggccg gaccgtcgcc gtcctggaac ggtcggatcg tcacgccgtc
     9481 gggtggccgg ccggcgctct cggtgccgtc gaggcggcgc tccatgtgcc agaacgtgcg
     9541 gacgtggcgg taccgcagcc gctccaggaa cgcgcgcccg tctccggacg acgagacgat
     9601 cggacggagg agcgtcgagt ccccagtctc gcggatggag cgccgagctc gaccctccgc
     9661 ccagccgacg aaccacgtgc cgagcccctc gccgcggtgg tcggggtgca cgttgatcca
     9721 cgcctcgacc gacgacgccg ggtcgatccc ccagcacgtg gcgcacgctg ccagcgagcc
     9781 atcctgcgcg aaggcgagca tcgtgtcctc cccggcacgg aagagcggat tcgcccattc
     9841 gtcctcgatg tgcccgcgga ccggctcgac cacgccgtcg tcggagcgat cgaccgcctg
     9901 cgcgaggccg gccatgcgat cgatgtcgtc tcgggtcgcc ggccggaccg tgaatccggc
     9961 cggcgggagg atcactcccc gacggccttc ttgcgggccg cggccggctt cttcgcgcca
    10021 ggcttcttcg cagcaggctt cttcgcggcc ggcttcttcc gggccggggc cttcttcttc
    10081 gtggcgggct ccgccttggc ctccttcttg gccgcggcga ccgaggcctt caaggcctcc
    10141 atcaggtcga cgaccttggc cgtgggctcg gcctcgacga cctcgatctc ctcgccgttg
    10201 atcttcgcct cgacgatgcg gagcatcgcc tctcgatact cgtccttgaa ttcgcccgga
    10261 tcccagtcgg ccgagaggtt gtcgatcagg gtctgggcca tgtccagctc gttcggacgg
    10321 accttcatcg agacgtcgac cccgccgaag tccgcctcgc ggatctcgtc cggccagtac
    10381 atcgtctcga ggatgaaggc gtcatcgcgg aagcgcaggg cggcgaggtg ctccttgtcg
    10441 cggaagctga ccttcgccac gcccaccttg cccgagcggt tgagggcctc ccgcagcagg
    10501 gcatacgcct tcgcgccggt ctcctcgggc acgaggtaat aggacttctt gtacagcatc
    10561 ggatcgatct cctcgagatc gacgaactgc tggatgtcga tggcccggga ggactcgacg
    10621 gggatcgcct cgaagtcgtc gtccgtcagg atcacgtagc gatccttctc gtactcgtaa
    10681 cccttgacga tgtgctcgta gccgacctcc tcgccgtcga cggagcacac gcgcttcatc
    10741 cggatccgcc cgccgtcgtc gtcgtggagc tggttgaagc ggagggtctt ctcctcggtg
    10801 gcgggataga cgctcacggg gatcgtcacc aggccgaagg agatcgctcc cttccacatc
    10861 gctcgaggca tgtcatcctc ccgtcgtcgt cggcggtcgc aagcacggat cgtgtgaacc
    10921 ccaggcgtac ccgggatcgt ttcatcctag tcgggctcgg ggacaccgcg gccgccggaa
    10981 cggcccggcc ggcccgctcg caggggatac cgtcctcggg cgtagcatcg agcgatgccg
    11041 ccccgccgac ggaccacgtc atccggtccc gcgttcacgc tcgacctccc cagggcactc
    11101 gaggcgcgca aggacggcga cgcgtggtgg gtcgaggtgg acggacgtga tctccgcctg
    11161 tcgaacctga acaaggtctt ctggccggac gagggttaca cgaagggcga cctcctcgcc
    11221 tactactgga acatcgcgga cctgatcgga ccgcacctcg aagggcggcc gctgacgatg
    11281 aagcggatgc ccgacggcgc ggagggacct cacttctacg agaagacggc tccctcccac
    11341 acgccggact gggtgcatcg ctgccaggtc cgctcggacg actcgaagcg aggccggatc
    11401 gactacctga tggtcgacga caccgcgacg ctgctgtacg tcgcgaacct cggctgcatc
    11461 gagatgcatc cgctgcactc ccgctgcgag gacgtcgcgc atcccgacta cctgttcttc
    11521 gacctcgacc cgttcgagcc gtacacctac gaagacgtcc tggccgtcgc tcgccacatc
    11581 aaggtcgtcc tcgaccagtt gggactgacc gcgtatccga agacctcggg agccaccgga
    11641 ctccagatct acctgcccgt ggagcgaggg acctacacct atgggcaggt gcgggcgttc
    11701 gtcggcgcct gtggtcggat gatcacgaag gccgaccccg accgcgtgac gatggcgtgg
    11761 aagatcgcgg accgcaccgg gaaggtcttc atcgaccaca acatgaaccg acagggcgcc
    11821 aacatcgccg ccgcgtactc gctgcgcccg gaacaccgcg cgcccgtctc gaccccgctc
    11881 acgtgggacg aggtgttcga gggcggcttc gagccacagg acttccggat cgacaacgtc
    11941 tgggaccggt tcgccgagcg cggggacctc ttcgcgggcg tgcgcacgga ggccatggac
    12001 ctctcgacgg cgttcgacgc gctcgacgtg cgacccgagg acgacggtcc cgggttctcg
    12061 tcggccctcc cgaggacggc ggctccttcc gtcgtccgga agacctccga ggagatcgtg
    12121 gaggagtcga gggacccgaa cctgcgcgag tacgtgcgga ggcgcgactt cggcgaggcc
    12181 ggcaccaccg agcccgcacc cggcgaggtc gccgggaccg gcaactcctt cgtgatccac
    12241 aagcaccgcg cgacccggct ccactacgac gtccggctcg aacgcgaggg cggcctcccc
    12301 tcctgggcgg tcccccgcgg gctgccgacc accaggggcg acaagcgact cgccgtcgag
    12361 accgaggtgc atccgctcga gtacggggcg ttcgaaggca cgatccccga ggggcactac
    12421 ggcgcgggcg aggttcggat cttcgacgat gggtggtatg agccgatcga gtggaccgac
    12481 tcgaaggtga gcttccgtct ccacggccgt cgctacccgg ggttggagtt ccacttcgtc
    12541 aagacgagga ccgattggct cgcgttcctg gcctccgccc aagacgctcc cccggtcgcg
    12601 acaccgccac ggtaccaacc gatgctcgcc gagggtggcg gcgagccctt cgacgatccg
    12661 cgatggcgct tcgagccgaa gctcgacggc atccgcgccc tcgccgagat ggagacgggc
    12721 ggcacggtgt tccgcacacg caggggtcgt gacgtcacgt tccagtaccc cgacatccac
    12781 atgatccacg agctcgtcga ccaggtgaac gccgtcctcg acggggagat cgtggcggtg
    12841 gaggacggag gccggacgtc cttcgaacgg ctccagcagc ggatgaacct ccagaacgaa
    12901 cgcgagatca agcgggtggc gaagcagatc ccggtctcct tcgtcgtctt cgacctcctc
    12961 tggttggacg ggcacgacgt cacggggctg gccctggagg agcgacggga gctcctcgac
    13021 ctgatcgtcg aggaggacca ccggctgcag ctcatgagct acgaggaagg cgacggcacg
    13081 gcgttcgtgg acggtgcgcg cgcgctcggc ctcgagggcg tggtagccaa gcgaacgggg
    13141 tcgaagtacc tcccgggcag gcgctccccc gactggaaga agatcaagct catctcgacc
    13201 caggactgcg tggtcctggg atggacgctc ggtcaagggg gacgggcggg gagcttcggg
    13261 gcgctcctgg tgggcgcgat cgacgacggc gcgatgcggt gggtcggaca ggtgggcacg
    13321 gggttcaacc accagatgtt ggaccgactg atggaacagc tcacgccact cgtgcgcgag
    13381 gaccccccga tcgacgaccc gtcgctgcgc aaggtcaagg gggcgaggtt cgtcgagccg
    13441 gagctcgtct gcgaggtcga gtacctcgag atcaccaagt cgtcgggcaa gatgcgggcg
    13501 ccgtcgttcc gtggtctccg ccccgacaag acgcccgacg agtgcgtgct cgaaccgccc
    13561 gcgttcgaca aggtccgtcg gaagcgatag cgcggcccgt cagaacggct cgaaccccgc
    13621 ccgaacgagc atcgcccgga cgtcccgctc ctccatgccc ttgccgacca actcctggta
    13681 caggcggacg gcgcccatcg agcgcccgca gcggggacag aaggcatgat cctcggcgaa
    13741 ggggacctga caccatgggc atcgcgtgag ctcgatccgg cgatcgggtt cgagccccgt
    13801 gtccgtgagc gcgtccacgt agcccttgcc gtagtcccac tggatgtact cctcctcgac
    13861 gatgtcgaac gctggctcgt gcatgcgtgg ctcgcccgac tgcagcatgt gctcgaggtt
    13921 gtcccggagg agctcccatt cgtagaagtg attcgcgccg cactcctgac acgcgatcac
    13981 gacgcccttc acgctctggc tggcgaagac cgcgcgcatc gaggagagat catcgagatc
    14041 cgactggatg tcccgtcgct cctccgcggt gagctcgtac ggctcgtcgc gttcgtcgtc
    14101 catcagtcgt cctcgtcgtc ggtcagccgg aacccacgga agccctctgc gaactcgcag
    14161 cccgctcgtt cccccacttc ccgggcgcgg gcgatgaccc gctcctcgac atccggtccc
    14221 agctgcgaag cgtgtgcgga cagcgccttg agcttgacgg cgagcgcgtc ggtgatgtcg
    14281 acgaacgtat ccgcctcctc cgccgacagg tagaggttcg gaacctcgaa cggctccagg
    14341 ccctcgtcct ccagctcctg gaacatcacc cgggtgggtg cgtccggcat cacggcgcag
    14401 agcgcgagct cgcccgcggc cttgtggtcc gcgtggttga tgtagccgct cccgctccac
    14461 aggcgtgagg gatcgggcgc cacgagcacg tcggggcgaa gccgccgaac ctctcgggtc
    14521 accttcctcc tcgtgtccgg agtcaccgcg aggaagccgt ccacctctcc gaggaacgtg
    14581 accgaagcca cgcccaggat gtctgccgcg gctcgcatct cccgctctcg gacctcgcgc
    14641 atcgcctcgc gcgtggtccc cgactcgttc gaccccgcgg atccgtccgt ggcacagacg
    14701 tagtgcacgt cgcagccttc ccgactccag cgcgccaccg tgccaccgca gaagaactcc
    14761 gcgtcgtcgg ggtgcgcgaa gaggaccatc gcccgagaga aggtcatcac cgggagggta
    14821 gcgcctcaca cgggggagca cgcgggccct ctgcttcacc cgcgtcggga gcacgccgat
    14881 accgtggaca tgctcaccga gcgactccgc gccacctcca tcgtgtggcg attcgccgtc
    14941 accagcttcg tcgtgttcgg tctgatcggt gttggcatcg gcgccctccg cgcggaggac
    15001 ctgcgaaccc gctccgagga ggcggccacg gtgcgagcgg agctgatcgc cgagagcgtg
    15061 atcgctcccc tcctcgtccc cgaggacctc cagggaccga tccgtggcgt gcgttatcac
    15121 gtcctcgatc gcgcgatcca cgagttcgcg atgaacgacg ccggcgtgga gcgcgtcaag
    15181 gtctggaacg tcgacggcat ggtggtcttc tcgaacgacc cggagcaggt gggcctccgt
    15241 ccggcgatgg aggaggatct gctcgaggcc ttcgaaggcg aggtcgcgag cgagatctcg
    15301 gacctgagcg aagcggagaa cgtgagcgag cgagcgctcg ccgatcagct cttcgagacg
    15361 tacgtacccg tgcacttgcc gggcgacggc gagtccaacg aggcgaccgc ggtcatcgag
    15421 gtctatcagg actactccgc gatccagctc gagatcgacc acctgaacga cacgctgaag
    15481 atctcgctcg gtatagggct cctggtcctc tacgtgctcc tcctgccggt gatggtcggc
    15541 accacccgga ccctccgtcg tcagaacgcc cagctgtcgg agcaggcgga tcagctcggg
    15601 gtcctgctgg cacgcgagcg ggagacggtg gcggagctcc gggagctcga ccgactgaag
    15661 agcgacttcg cggccgccgc ctcccacgag ctccggacgc cgctgaccac gatccgcggc
    15721 tacgccgagc tcctcaggga tcgagggcct gccgacgatc ccagcacgag agatgcgatc
    15781 gacgcgatcg ttcggcagac ggggcacctg cagcgcctcg tcggcaacct cctccgggag
    15841 gcccagctcg agcacggcag ccacgagacg cgggaagagc ccacgtcgat cgcagacgtg
    15901 ctcggcggcg tccgccgagg attcccgggg tccgtaggtc gcatccatgt ccggaccgag
    15961 ccggacatcc cgagcatgct gctcgatccc gtgacgttgc acgagatcgt cgccaacctc
    16021 gtggacaacg ccctgaagta ctccacgccc ggcgccccgg tcgaggtcac cgccgaggtc
    16081 gaggggaacg cgctggtgat ccgcgtccga gaccggggcg aggggatccc tccgggcgac
    16141 ctccctcgga tcttcgatcg cttcacgcag atcgacggat cctcgactcg ggcccacggt
    16201 ggtgtcgggc tggggctgca tctcgtccgg gagctctccc ggcgctccgg cggtgaggtg
    16261 gaggtggaca gcgtgatggg catgggcacg acgttcaccg tcacgctccc catctcgacg
    16321 gccggtcagc cggatccgca gcgccccgag atgacgacgg cctgagctcc tcgctcgaac
    16381 ctgtggggcc tgtggttcga tagcggcggt ggccgacggc gaccggatca gcgagctcga
    16441 gcggtccttc cgacgggccg gcctcccgac gttcgtccgc ggctactcgg cccggcgtgc
    16501 cttcgcgaag gcgctgccgc tcctcgcgat cgtcttcgtg ctcgagatcc tgaacgcgtt
    16561 gaacttcgat ttcgggttct ggacgaacgt ggggttcctg gccggtggga tcgcgatctc
    16621 gctgggcatg atcgcgatcc tcaacctggc gcgtgggcag cggttcctct cgatccctcg
    16681 gctggtcggc gcgccggaga tgatcgtgtt cgtgctggtg ccctccgtgc tccccttcct
    16741 cttcggtaaa cagaggacca gcgcgatcgt cacgctcgcc ggcaacacgg cgctcctcgg
    16801 tctcgtctac ctggtgttgg ggttcggcgc tgtctcgatc ctcgagtggg cggtgcgccg
    16861 gttcatgagc ctgttcgccg catcgctcac ggtgctcgtg cgtgcgctct cgctcctcct
    16921 cttcttcctg ctggtgatct tcttcacgac cgagacgtgg cagatctgga ccgtgccggg
    16981 actgccgaag ttcttcgcgg cagccggatt gttcgtgctc ttggccgccg gatccctgct
    17041 gctgcggctc ccggggagcg tccgcggcct ggaggccgag ctccagggcg agcgcctcag
    17101 caggacacaa cgcgtgaacg tcggcctcgt gatgttcctc agccagttcc tgcaggtcgt
    17161 cttcgtggcg ttcgcggtgt ggctcttctt cctcgtgttc gggtcgctcc tggtgagtgc
    17221 cggggttcga gaggcctggc tggggacgct cgggaccgag gtcgttcgga tcccgttctt
    17281 cggcgacacc gtggtggtca tcacgcagga gctcctgcgt gtcgcgacgg gcatggcgtc
    17341 gttcgccgcg ctgtactacg ccgcggcgac ccagctcgac gaggcctatc gggacgaagt
    17401 ggtcgaacgg atcgccgagc agatgaagga gacgttcgct cgccgcgccg agtacctgcg
    17461 actggtgggt ggatcgcctg cccacgagac ggtcggatga gacgaggaac aacggatccg
    17521 cccccctcac cgcagccctg gcaggtgtcg gccccatcgg gtacgctccc tgtcgagggt
    17581 tcatgcagca ccggttcttg tggcaccgtt gttctggcag ttccctcccc ggccgaggcc
    17641 gggtaggccg acggacaccg cttcgcgatc aggtccgcac cggccgcgga aggagggaac
    17701 gccagtggca acaggcaccg tgaagtggtt cagccccgag aaggggttcg gcttcatcac
    17761 ccagtccgaa ggccccgatg tgttcgtgca cttctccgga atcaccgggg aggggtacaa
    17821 gaacctcgag gaaggacagg cggtcgagtt cgacatcgtc gacggacaga agggccctca
    17881 agctcagaac gtccggatca cctgaaccac ggaacctgaa gcgaacggag ggggcggcgc
    17941 gcgaagcgcg ccgccccctc ccctgttcgg gtcagtcccg gggtcgatcg aacctgagat
    18001 cggcgagcag gccctcggtg cgctcgaccg cggacgtgag ccacgtgccg tcctgcgatc
    18061 cccgcagctc ctcgcgcgga tatgcgaact gattcagcgt ctcgtcggag ccgacctgga
    18121 tgcccttggt caacaggtac tcgccgagcg cgaactccca gctgaagatg gcggtcccat
    18181 ccggataggc gtgcaccgtg tacgtcaggt acggagtggg ctgggcgaca tacagctgcg
    18241 cccctctccc gaacccacgc tcctcccgca gccggaaccc gaagcgctcg agggcgtcgc
    18301 cgaactccat cggggtcagc ctaccggccg gtcgtcagcg aacgatcgac aggacctcgt
    18361 cctcgctctg cgcgtccatc agctgcagct gctcggcgtg ccgcgccctc gcgatggagc
    18421 cgcgcgcctt gccgaggttg gagcggaact catcggcgcc gttcgcgatc atcgtgccgg
    18481 ccttcgagaa cttgttctcg acgatcgcgt ccatcgcgtc gagcagcgtc ccgacctcgg
    18541 cgagcgcggc ctgcacgtcc gcggcatcgc cgaagcgctc gacgagcgcg tagaggaaca
    18601 tgatgatcgg cagcgcgctc gagtacggca cgatcaccac gccacgcatg aatgcctccg
    18661 tgtgggctcg cgacagaacc tggtacgcgg catcggggac ggctgcgacc gcgaccggcg
    18721 cggtcaccga cgcgtcgatg tactgcccca cctccttggc ccgcgcggcg acggccttct
    18781 ccaccttgcg ggcgcaggcg tcgcgtcgcg tgggatcctc ctcggcctcc atcgcttcga
    18841 gctccgccac ggccgaccac ttggaatcga tcggcaggcg ccggccgtcg ggcagacgga
    18901 gaccgaactc cacgaccttc ccgttcacgc ggaaatccga ggcgagcatc gacggaggca
    18961 gctgcccgag gtgctcgcgg aggacgtgct ccccggctcg gcccttggtg ttccctcccg
    19021 cgagcacggt ggccaggcgc cgcacgacct cgcggttctc ggtctcggag gtccggcgtt
    19081 cctcgtcgcg cacctggagc tgctcgaggg cgtggcgcgt ggcttccagt cccgcccgga
    19141 ggtgatcggc ggagacgtcc cgctgacctg aggcgtcagc gatccggcgg agctcggctg
    19201 cctgcacctc caatcgtgcg gtgagctccg cgtgcagctg ggcgttcagg gctgtctggg
    19261 cgtccagggt gctcacggag tccggatcct gctgctgcgt cggctgccgc cggagcatca
    19321 tcaacgcgcc caccgcgatg cccacgaacg cggcgagcac cgcgatcacg acgtcgttcg
    19381 tcacgggtcc accggtgaag gggtcatgag ggggatcatg gggcgaaccg taccccgagg
    19441 gtgtgacaga agcgcgcaac cgagcgcaag tccgggatac catccatccg tgactccgca
    19501 tccgatcgag ctctaccgtc tgcgcggcac ccaccacaag gtggggatcc agatgggcga
    19561 gctgggagcc gaccagatcc acagggccct cgggtccttc gatgccgatc ttcccgccgg
    19621 gaggggccgg accgagcagc tccggctggc gcaggccttc cgagacgtca cggcaccgtc
    19681 gctcccatgg ctgatcgagg agctcgacgg ctgcgcggaa ggcgccggcg tggacccgct
    19741 cgagttcttc gcgacgaccg tcgaggagct ctggtactcc ccgtatccga agcggaccca
    19801 gggccggtgc agcgacgtgg tggccgggcc ggcggcgacc gcggacgggc atctgtgggt
    19861 cggccacaac aacgatctcc ggccggaggc agaagccgac atcgtcgcga tcgagaaggc
    19921 cgtggaggac gagccggtgg tcctgcagat cggtggcgtc ccgtggctca gcgtgggatg
    19981 gaaccaggca ggcctctccc tcaccggcaa cgaactcagc ccgaacgacg agcgggtcgg
    20041 gatctcccgg tcgcaccagg tgttcgagat gctgcgcgcc cacaacctcc acgagatggt
    20101 ggcctatgcg atccggcccg atcgcgcctc ctcgtacaac aacgtgctcg ccgatcggtt
    20161 cggcgacgtc gcgaacgtgg aggggagcgc gacggccgtc gagatcaccc acctcgacga
    20221 gcgcgaccat ctcgcgcaca cgaatcacta cgtgagtgag cgcatgcagc cgtacgaggg
    20281 cgacccgggc tacgccgtcc gctcggacgt ccggttctgc cgggcacggg acctgctcgc
    20341 ggaccggcca ccaggatccg tgacgccgga ggtgcttcgg gagatcctct ccgatcacga
    20401 gaaccagccg aacgaggtgt gccggcatcc cgagtggggg catcccacct ccaagaccgt
    20461 tttctggtgc atcgcggacg tcacggaggg acggatcgcc tacgggcgcg ggaatccgtg
    20521 cgactcgatg gagcaggagt atgccttcgc cggctacggc gagggctgaa gcgccgagcg
    20581 gacccgctcg atcgtggcgt catcgccgct ctcccccgcg aggtccgcga cggtgcgccc
    20641 ctcgtcattc gtcgcggccg ggtcggcacc ggcgcggagg aggagctcca tgctcgggag
    20701 gtcgccgttc atcgcggccg cgtggagggg cgtccatccc gccgactggc gtacgtcggg
    20761 gttcgccccc gcctcgacca ggatccgaac gacgtcggac tggaggcggc tcgccgccga
    20821 gtgcagcgcg gtccctgtca tccatccccg gccgaacgcg tcgacctcgg cgccccgttc
    20881 gatcagcagc gccgccgcct cgggctgccc gaagaacgcc gcgaagtgca gcgccgtgaa
    20941 cccgtcgccc gagtaggtcg tcgcagcggt cggctccgag tccaagaccg ctcggaggcg
    21001 atccacctca ccgagcgccg ctgcctcgaa gacgtcgggc gcgcccgcgt gcgcccgcac
    21061 gatctccacg aggtcggtgt cgaaccggta gagcgcccgc atcaatgccg agacgcccgt
    21121 ctcgtcgcgc atgtcgcctg ccgcaggatc cgcgtcgagg atcgtctcga ccccgccgag
    21181 gtctcccgct tcgatcgcgg cgaagaggtc gtccgaggtg cttgccatcg tgtcctccct
    21241 cggtagactc gatcgtatat ttaggtagcc taacgaaata ccgatgacca cgtcaaggcg
    21301 gacttccccg acgcgagagg tgggcgaggt cggccgagtc gccgatcggc tgcactcggc
    21361 caccatccat ctcctgcgac gcctgcgcgt ggaggacgag gcgctgggca tctctgcccc
    21421 gcgcctctcc gttctgtcgg tcctggtctt cgcgggcccg aagcggatcg ggaagctcgc
    21481 gcggatcgag caggtcgagc cgccgacgat gaccaagctc gtggacggac tcgttcggga
    21541 cgggctcgcg ctccgcgagg ccgacccgga cgacgcgagg gcggtccggg tccgcgccac
    21601 gccgaccggc gcgcgcaccc tccgccgagg acggaagaga cgcctcgaga cgctgcagcg
    21661 cacgctcgag accctctccc ctcgcgacct cgctgccctc ggcgagggcg tcgacgtgct
    21721 ccaacgcgtg ctgggcgacc gctagaccgg atcgccggcg cgccacacac gcttccgccc
    21781 cagcagcgcg cgcggatcct ccacgggatc tccgtccacg agcaccaggt ccgctcgctt
    21841 cccgacttcg atcgtcccga cgtccgcgag tcggaggagt tcggcgccgt tcgccgtcgc
    21901 ggcccggagc gcgtccagag gtgccatccc ccactccgcc atatagacga cctcctgggc
    21961 cgcgttgccg tgcgcgttga agggcgtgcc ggcatccgtt ccacagacgg gacggacgcc
    22021 cgagcggacc gcgagccgca gcgagctctt cgagtcctcc tcgatcgctc gtgccttctc
    22081 caccgcgtac gcggccaccc ggtccgcgtg atcgaggatc cctctgaccg cacagatcgt
    22141 cggcgaacgg aacgtgcctc gcgactccat ctcgcgagcc gtcgacgtgg tgagctgatt
    22201 gcagtgctcg atcgagtcga cgccggcgag cacggcggcg cggatcccgt tcgcgccgat
    22261 cgcatgcgcc gccaccgaca ggttccgctc gtgcgcctcg cgcacgccgg cctccagctc
    22321 ctccgccgtg aatgcgctga acgtggccgg gatgcccggg gtgaggacgc cgccggtcgc
    22381 gatcagcttg atcgcggcgg cgccggcgcg gatctcctcg cggacggcgg cgcgcacggc
    22441 atccgggccg tcgacctccc gcgagaacgc gatgttgtgg ccatgaccgc cggtgaccgt
    22501 gagcgcgcgc cctgccgcga ggatccgcgg ccccgggacg agcccggcag cgacggcccg
    22561 agcgacgtcg atgctcgcac cgcccatccc gcccagatcg cgcacggtgg tcacacccgc
    22621 gtccaggttc ctcttggcgt tcaccatggc cttcagcgcc gcgaacccgg gcgtggtgag
    22681 gtcccgcgct tccttctcga agtccgcctc accatcgaac tggaggtgca cgtggacgtc
    22741 gatcagcccc ggcagcaagg tcttcccgtc gccctcgacc acacgggcat cccgcgcatc
    22801 cttcggcggt ctcggatgcg cgccgaccca ctcgatccca tcggcgccga agagcactcc
    22861 ctggcgcttc ttgaccttgt tcccgtcgaa gacggtcacg ttcgcgacgt agatggactt
    22921 cgccatcgca gcatcatgcc acctgccgaa ggcgctccag ggccccgtag tacgctgacg
    22981 tcaccagagg cgcgagccag aacatgagga ggatcgatgc tcgtcggcga gatcatgcat
    23041 ccggacgtga agaccgcgag tccccaggat tccttcgccg acgtcgcgaa
//
LOCUS       DASUYD010000016        27465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_451765, whole genome shotgun sequence.
ACCESSION   DASUYD010000016 DASUYD010000000
VERSION     DASUYD010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27465
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451765"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF195_00870"
     CDS             complement(<1..234)
                     /locus_tag="VF195_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6579420.1"
                     /translation="MGTQVGVGAGQESSEIRRGVGATVGVLAAGVALGTAELLSAVFG
                     PASSPIVAVGGAAVDATPEWLKSFAIRTFGEHDK"
     gene            330..1895
                     /locus_tag="VF195_00875"
     CDS             330..1895
                     /locus_tag="VF195_00875"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6579421.1"
                     /translation="MSRRLLALVSSLGLVILFFTPTARAAPVTVKVMTLNVFYGGDEW
                     NLATGQWCADPAGCPETMDHVVSTIRAADADIVGLQEGTANECVIADRLDWHCEPRLQ
                     LISRYPLHDPPGADGAYVLAEVTPGRFVAISNVHLPSDPYGPYWVRDGEPRAAVLDLE
                     RTTRLPAIQRELDVLPELADAGMPVFLTGDFNSPSHLDWTPAVDAVREEVRYPVVWPV
                     SAALAEAGFRDSYREVHPNPVAVPGFTWTPPGTLESVPDEVHDRIDWVLVQGPAETLA
                     TQLVGEAGGPDVDIERDPYPTDHRGVVSTFRVEAADPALFAAVGERRVFIGRDLDVRA
                     HGPGSRVVLVRAGQGPAAALVSRPLGGASDVTLTFPTGTLSPGAHEALLVNASDAVLS
                     RSPFWVYAAGTPPSLRTSKEVYEVGEPIRVRWRAAPGYRFDWLGVFSPGRGPAQNAED
                     CNAGTCGNGHYLLYDYTETLIQGSTLIGPDSFAGYRTWPLKVGNYEIRLLMDDGYRLL
                     GISNRFKIVHASA"
     gene            1980..2864
                     /locus_tag="VF195_00880"
     CDS             1980..2864
                     /locus_tag="VF195_00880"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX6579422.1"
                     /translation="MSPIVIIVLVVVALIAVAYFAGLRPAPVSGRRPTPGLLRRRSKW
                     RKVLPFLPLLAAVGCLVYAFSGFRLAVKEASPVIVLVLDVSDSMNATDVAPDRLSAAE
                     TAAIAFLDELPPEFRVGLTTFAGAAQLVVSPTRVREEVVNALADLTTSKGTVIGDGLT
                     VALDAIEELRSEAPDTSAAALLLSDGNDTGSRVTPGQAATRAASLEVPVFTIVVGRVT
                     EGDGHGADLSALEAIANTSGGDTFTAETSNELTSIYTDLGSALSVDLEVAPSTTPFVV
                     AAIALTVLAGLMLVYLPR"
     gene            2975..3394
                     /locus_tag="VF195_00885"
     CDS             2975..3394
                     /locus_tag="VF195_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018381552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6579423.1"
                     /translation="MGQQVKEIMTAQPVTLDRDATIAEAAKLMRDRGIGDVIVVDGED
                     AQGIVTDRDIVIRGVAEGADPDTTRLGQVVSGDLTSVAPDDPVERAIELVRGKAIRRV
                     AVLDGGKPVGIISIGDLAIERDPRSALADISEEPPGK"
     gene            3400..3645
                     /locus_tag="VF195_00890"
     CDS             3400..3645
                     /locus_tag="VF195_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579424.1"
                     /translation="MDGTRTTTRDIESTRREMHPERPPETGLLLALDEAVGALRRAQP
                     PFLLMGGLATSILGVAAASRTSICSFTTVTSRRSSRS"
     gene            3630..4025
                     /locus_tag="VF195_00895"
     CDS             3630..4025
                     /locus_tag="VF195_00895"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEX6579425.1"
                     /translation="MLAVLKAAGFETMVVAPNWLAKGFKDGVLVDVISRSTHDIVLSE
                     EILEHAIEIDVEGRRLPCVAPEELIVMKAIATTEDATRYWYDALGLLGRPDLDWEYLV
                     RRAKQHGARRVAALLFFAQSIDLLSSPVH"
     gene            4094..4342
                     /locus_tag="VF195_00900"
     CDS             4094..4342
                     /locus_tag="VF195_00900"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6579426.1"
                     /translation="MDDPRYEQERIRQALATDARVGEPELGVDLVAGRVLVTGSVPNE
                     RVRAAIDLVVAEIAPGAALDNRVTVPPADEPTGEETIG"
     gene            4339..5087
                     /locus_tag="VF195_00905"
                     /pseudo
     CDS             4339..5087
                     /locus_tag="VF195_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209233.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            5144..6172
                     /locus_tag="VF195_00910"
     CDS             5144..6172
                     /locus_tag="VF195_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX6579427.1"
                     /translation="MQDLTLERMADVRAEALPSARGPLSARMLDLLTDRSVGRHPAGV
                     PDDPLGDDDLHLALGLAYELSYRGLPGVRDDLEWDLGVLSARRDLEDAFEAGLRHTIG
                     RESVDPRDVPDRLREVATPPDADSLSAFVEREATLDQAREFLMHRSAYQLKEADPHSW
                     VIPRLVGAAKSAVMEIQYDEYGSGDAAWMHSELFRTTMIAAGLDGSYGVYLDRLPGPT
                     LATVNLITLFGMHRRWRGALVGHLAAFEMTSSLPNRAYAQGFRRLGFDDDAVRFFDEH
                     VEADSVHEVLAGDLAAGLAAAEPELASDVVFGAVAMALLEGRFADGLLRAWRAGDSAL
                     LAVAFRSS"
     gene            6169..7146
                     /locus_tag="VF195_00915"
     CDS             6169..7146
                     /locus_tag="VF195_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019182064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX6579428.1"
                     /translation="MSEDLSYVLPYRSRPGDGNEDELDRYLRWLSGRVQVIVVDGSEP
                     DRVRAHRERWARIATHAVPDADLSCRNGKVRGVLTGLRRAQYEHVIIADDDVRYDDEA
                     LLRMQELLRNADLVRPQNVFDPLPWHARWDTARMLLNRALGNDHPGTLGVRRSFLASI
                     GGYDGDVLFENLELARTVLAAGGRVVDAPGMYVRRLPPTPARFMEQRPRQAYDDLAQP
                     ARLGFFLVLGPALAVVARRRPQVLLAAAIASILVAQVGRIRHGGNRVFDRVSPFFAPV
                     WIVERACLVWLAVFRRFSRGGCTYAGDVIRRAATPPRVLRRRLRGRLGG"
     gene            complement(7079..7336)
                     /locus_tag="VF195_00920"
     CDS             complement(7079..7336)
                     /locus_tag="VF195_00920"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6579429.1"
                     /translation="MSRRAEVGVVRVTADGPLLVRGPIRLVDERGDEVRIRRRVNAIC
                     RCGRSATQPFCDGTHKIVPFSPRAVPAASAEGPVGESPLAG"
     gene            complement(7333..7572)
                     /locus_tag="VF195_00925"
     CDS             complement(7333..7572)
                     /locus_tag="VF195_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579430.1"
                     /translation="MPDKRPSVKNEDQYEALKKKGMSKERAARIANSPGASARGGKAS
                     GSETRRRTTSQGGTTAQKKAAGRKGGRATARNRSA"
     gene            complement(7632..8009)
                     /locus_tag="VF195_00930"
     CDS             complement(7632..8009)
                     /locus_tag="VF195_00930"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6579431.1"
                     /translation="MSESNRTAKLLERERARLNEIRSSLTEVAEDVERDPTGDPSAGG
                     HVADAGSEMFERARDLSIISEIDAQLTDIDHAEARLSNGTYGTCEACGKAIGPERLAA
                     RPATRFCIEDQIGVEREMQARGA"
     gene            8246..10348
                     /locus_tag="VF195_00935"
     CDS             8246..10348
                     /locus_tag="VF195_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6579432.1"
                     /translation="MIGLTMVSLLAVPFAPAGAAPRSQDPQVGGARLTDSDGDHLDDA
                     LERRLRRSERTTRHAVIVATDGSLDLAGAHRVAGAFSIGRRLEIVGGFSAKLTAGQIR
                     RLGSTPGVLRIDHDAVIRATMDAARADYGVESARADLGLTGAGSTVCILDTGADPDHE
                     QLDSKSIVWKDFVGTAATPVDDHGHGTHVASIAVGDGVGGPDAARFGGVAPAAGLWVG
                     KVLNAQGSGSESGIIDAIDWCVDAPEVDVISMSLGSELPSDGSDVLGIAANNAVASGK
                     IVVAAAGNGGDAPDSMASPGAASDVIAVGAASSWSAAPGVVNRSNGIYLAPFSARGGP
                     TFAADPKPDVVAPGVNVRAADANTGSGYATLSGTSMATPFAAGSIALALEAWTGSEPT
                     PADVQSAIEGTAEDFGPAGKDADWGAGLIDVLALASQAAGASGATDFPTHEHLSGSVP
                     DSGEWTHAFEVGPDDLDVPIAASIVLDGSCEDFLGFGCLFGWAPDIDAELLDPTGELL
                     DVSVCAAIEAGDPSPGPCSIGRQETLHAMPTVAGTYLIRLFPFGGSPNDGLGGAFDLD
                     LSTGPVGDDTTPPPPPPPPPPPPPSIHVGDLDDVSITLSSTRWRARATIRVHDGSHAA
                     VSGVLVQGRFGPNGSTLSCTTGATGACTLKRDLKRTRLSIEFVVSGLSKEAHTYLAAS
                     NHDADGDSSGTKIVVTRP"
     gene            complement(10395..10931)
                     /locus_tag="VF195_00940"
     CDS             complement(10395..10931)
                     /locus_tag="VF195_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579433.1"
                     /translation="MRATHQHHDADATSTETRAVSEAPSPWLALRVLLTVAAAVLLLV
                     GSFFEWAGGTDGSEVAVQAFWSTDAGTAGFFSSAGLVTAICGAVIILGLAFRGGWLTR
                     FAGAVALVAFTLIVIQMGREGSLDTLQDVGMGLWFVLAGGILALIGGFFPSKRTVAAA
                     TASTDTDTDERATHSHTD"
     gene            complement(11074..11442)
                     /locus_tag="VF195_00945"
     CDS             complement(11074..11442)
                     /locus_tag="VF195_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579434.1"
                     /translation="MGPTRVSTSRLSVAGDPSLALVVRIFVGSVTEQWEVPEPVRDDL
                     RLAASELFSGAVEAGNRDIRFELSTDEVGMELRAEDVAVEQESADLEGWTGRFALIRA
                     LFPDADVSDTRVRIRVPLTN"
     gene            complement(11442..11780)
                     /locus_tag="VF195_00950"
     CDS             complement(11442..11780)
                     /locus_tag="VF195_00950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6579435.1"
                     /translation="MDLTLDRRTHGRWAVVEVKGELDLYTAPSFRESVLEAGDAEDPK
                     VIVDFRGLGFIDSSGLGAIVACLKHIRERGGDLALVAPEGSGLRRLMDLTGLDRVLTL
                     YQSIEDISQD"
     gene            11839..12234
                     /locus_tag="VF195_00955"
     CDS             11839..12234
                     /locus_tag="VF195_00955"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579436.1"
                     /translation="MKNLPTAVCVDVPATAVFIQVLRNVVAGVAARLDLPIDEIEDTR
                     LAVTEAASLLLEEGDATSLKMSIGRDGDTMEVTLTSDGRSDPWPTDRVTASWPWLVMK
                     GLTDEVRADRAEGAGPSIGFTKRRVRVDR"
     gene            12231..12977
                     /locus_tag="VF195_00960"
     CDS             12231..12977
                     /locus_tag="VF195_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HEX6579437.1"
                     /translation="MSVRTGQTRSDEEIMELFRRLPDPAAREALATEFLPLAEYFARR
                     FSGRGEPVDDLTQTASLGLLNAIDRFDPERGVPFSTYAAATMVGELKRHFRDRGWALR
                     VPRNVQETAILVNRTVSSMWQDLGRAPTVAEIARAADIGEDDVLQALDALQAYTTDSL
                     DAPVGDASTTTAESIGSVDPSFQVSDDWLSIAPALRDLPERERKILYLRFFEGRTQTE
                     IAGELGISQMHVSRLVSQSLEKLRKLAEEV"
     gene            complement(13038..13532)
                     /locus_tag="VF195_00965"
     CDS             complement(13038..13532)
                     /locus_tag="VF195_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6579438.1"
                     /translation="MMLVRHAMAMSPVTASPDMNAGDAAGLMAANDIGVVPITDGDRL
                     IGIVTDRDLALRVLAERKDPKTARLGEIATTKGIETTSPDTQLCDARALMAERRIRRL
                     PVVKDGRLVGMLSLGDVAIGSASMRSVGETLASISTSMATVDTNAGPEIGTPARVKEA
                     RETS"
     gene            13646..14170
                     /locus_tag="VF195_00970"
     CDS             13646..14170
                     /locus_tag="VF195_00970"
                     /inference="COORDINATES: protein motif:HMM:NF039409.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6328 family protein"
                     /protein_id="HEX6579439.1"
                     /translation="MTEERQNRRAGSQADRGAAATTDPDESPKERVDRELIELLNGLR
                     VILPGVQVLLAFLLTVPFSSGFEDATSTDKIVYFAAVLLTVGATGALTMPAAYHRIRF
                     RQGDKERMLRLSNTFAIVGLALLTLAMTTVVFLITNVLYGVVAAIPVSLVVLLVLGLS
                     WFAVPVFRRVEDAG"
     gene            complement(14218..14556)
                     /locus_tag="VF195_00975"
     CDS             complement(14218..14556)
                     /locus_tag="VF195_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579440.1"
                     /translation="MSGSQADEDRFEPRLKRRLSTGLVVGSLVGIAIGIAIGIFAFDG
                     RAGAVAACAIAGLIAGLLYGTIVGSFAGLESPDPGQEPSETPHPLSEPVVDEEREPDG
                     PGVRGAYPPD"
     gene            complement(14750..15115)
                     /locus_tag="VF195_00980"
     CDS             complement(14750..15115)
                     /locus_tag="VF195_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579441.1"
                     /translation="MRQPATIHEAKSMVKERFLDRRVEKLSKENDKLREAVGELRQDL
                     DDAQDRSKETLAALSKTRRPGRIKWLVLAGGAYVLGAKAGRARYEQLKGWARSLTPEN
                     GNGTTVTEVTTGPDLPSKA"
     gene            complement(15184..17352)
                     /locus_tag="VF195_00985"
     CDS             complement(15184..17352)
                     /locus_tag="VF195_00985"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6579442.1"
                     /translation="MSMDPTERWAERRSVRKPHSPGPLAPWIWNPRWIPGFVLAQVAV
                     LLVFAEKSPGSFEGVPGGFGALLSVATAIVCGPIAGALVSLVGGLVFVPLVTDFERGS
                     QFSVFLWLIASVGSGVMAGRLRQANGERTSAIARERLAGNRLHRLQAVTEVLARATNP
                     EDVANATVSAAIEALGADVGGLTIRAGDDQGILRVLVGSALDEPADTGWKRHATDRPA
                     PGPEIVRTGRPIFIETRDELLARFPIVRKIERGAARYGGFAGVPVALGTEVLGALSLG
                     FHRDHMVPPEERGLLLSIAHQAAIALDRIRSSDDERHARATAEHAEDRLRKLQAVSDA
                     ANAASGLDELVERVLPVVRDAVLADGTSFLLLSDDGRELRFKGQVGEAEEASEVVRIP
                     LGAGASGRIAATGRPLVIGEMTPDDVISPALRGRRSYVGVPVRVSGRVVGVLHASSAT
                     PGAFQDDDADFLQSIADSLAATIDRTRLFDQRDRMATALERALLPMSLPHVPGFEIAT
                     MYRPSRFGDEVGGDFYDVFGDGKVWYLAIGDVCGKGPEAAAIMGMTRIALRSLAREDD
                     TRPLPEVLGLLNGFLIESELMGDRFCTVCVVRLTFSDDRAAVTACLGGHPPPLVIRAD
                     GSLDAVGRPGSLLGLFEDISLYEAHADLGRGDAITLFTDGLTELSVEHPDEGEAMLRS
                     ALVAAAGEEAAGIIKTVERTLLDPRGELRDDAALVVAKRL"
     gene            complement(17352..17735)
                     /locus_tag="VF195_00990"
     CDS             complement(17352..17735)
                     /locus_tag="VF195_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579443.1"
                     /translation="MEHRATFELAPEAASEARAVVNAELGPAVSTKVLEDATLLVSEL
                     VTNAVRHAPREGLPKIELRLKVDPARVRVVVSDPGTGFVAEPRLPTASESSGWGLYLV
                     DRLADRWGIITRDRSEVWFEIDVGA"
     gene            complement(17845..18048)
                     /locus_tag="VF195_00995"
     CDS             complement(17845..18048)
                     /locus_tag="VF195_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579444.1"
                     /translation="MQLSVELDPTSVRTETPQDGPPVPRFDEGRVCEVADCRTVLSVY
                     NDGERCWSHRPMERVTPLRNHRK"
     gene            18200..18532
                     /locus_tag="VF195_01000"
     CDS             18200..18532
                     /locus_tag="VF195_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006503155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEX6579445.1"
                     /translation="MPQQAWSEKRERQYEHIKEGLRKRGRSEDTAEEIAARTVNKERA
                     RSGEARTASRTSVDDLSSGRRGGLRSHSGPGGRTYRQLYEEAKDRGIDGRSRMSKAQL
                     ERLLDRRR"
     gene            18880..19272
                     /locus_tag="VF195_01005"
     CDS             18880..19272
                     /locus_tag="VF195_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579446.1"
                     /translation="MSFRLPPDARSPAEARRTLEALRVSLDDPVVDNAALLVSEVVSN
                     SVRHAGLGAADAIEVRVRGSRAMLHVDVIDPGPGFEPQVRPREDDGGWGLWLLDQLAT
                     RWGVEQGDTTRVWFDLAPPVSAASASGT"
     gene            19313..19507
                     /locus_tag="VF195_01010"
     CDS             19313..19507
                     /locus_tag="VF195_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018534695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEX6579447.1"
                     /translation="MGENMDKAKGHAKEAVGDLTDNERLEREGKLDQASGDVKGAAED
                     AKDKVGEGVDAVRDRLKNDD"
     gene            19623..20258
                     /locus_tag="VF195_01015"
     CDS             19623..20258
                     /locus_tag="VF195_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579448.1"
                     /translation="MPENGDHPADYTGLHAFCFVDHVEPGTNPEEVVRRLIAKGKPPI
                     MYASTVVGDYQVFVHARVRRLADLQDLLEGVIFEAGARCTWAIESPIRRAGAKRKSPG
                     LIALTRLTMEHGTENAARKKLVTPPLSTGFVGASKITGAHGILLQMTGSSIIDAERNI
                     RAALQDVEGVLGMSTAFIDGDRTLERYGEIPLNEMEDEEPEEPDAAEGEWS"
     gene            complement(20197..23397)
                     /locus_tag="VF195_01020"
     CDS             complement(20197..23397)
                     /locus_tag="VF195_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579449.1"
                     /translation="MQLCPSCSADNPEHARFCLACGHAIDATEPAESRRQVTVLFTDL
                     AGSTDLTHRLDPESLHRVIGGYFDAARTAVGRHGGHIEKFIGDAVMAVFGFPRANEDD
                     ALRAVRAAWDLRSLIDEANDDLRSKWDVELAIRTGIATGNVVASDRSTGEPFVIGTSV
                     NLAARLEQHAGTHEILLDGVTRRIARKAIETEEVLLDELRGFDGPVKAHRLLKVLETG
                     EGLPRPEPPFVNRTAELRLLHETYESHVASSRCRTLTLIGSAGLGKSRLVAEFLREHP
                     QAVVLRSRCPVYGEGSALQPLRDVVAQAAGSGPEDDPVSIVEKIRDLLVEQPEGSLAA
                     DGIARALGLAPGTTTQEETVWSIRICFETLARRGPLVLAFDDVQWAAPALLDVLEHLA
                     EWARGAPILLLCMARPDLLDLLPTWGRTPGAVMLRLEPLGAAPSRELARHLLASTAAP
                     VFEDTIADVADGNPFFLEEIVTMLEEEGAITPDATPGDLSRIAIPPTISALLAARIDR
                     LDPAARQVLERAAVLGLAFARAHLEALLDGEDAIDVDATLRELAQRDFVVADAEMPGD
                     AYRFRHALTREAAYEAIPKSLRVRLHTAAAERLAREPAGEARDEQVGYHLEKAHHAVR
                     DLSETDPRAQPLAERAGTHLAAAGRAAAGRGDVRAAAGLLERAAELLPEMHAERAGVL
                     MDLHEALLFAGEIERSQGPVAEVLASLAPDDDSVIGERARLQQTMLRFLLDPGATPAD
                     VLRGDLERSVRRFEDAGDEASLAAALADLATIHWFEGDAEAMLEAAQLALAHARASGS
                     RRSAAEAAPLIAYALHKGRVPLDEALERLDETRAQLDDDLLARTLLLLDEAMMLAAVG
                     RQGDAKVATDRARDTFEDLGQRRWLEISKAAHAEIARREGKLERAEELLRSVYDFFRA
                     QGDENNSLQIAFDLADVLCDLGQFDEADLLATLVAREGPPDDIEVQVSWRAVRSRTSV
                     ARGDPTGAVTLAEEAVSIADTTSFVLLQAEAARALGEALHGAGRTAEAAAALGVALER
                     YESKRAAVPAAEVRERLRTILLPPRPAPPAPRPPSR"
     gene            23542..25161
                     /locus_tag="VF195_01025"
     CDS             23542..25161
                     /locus_tag="VF195_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6579450.1"
                     /translation="MASYDAIVIGGGHNGLTAAFYLARAGLRTVVLERREIVGGACVT
                     EEIAPGCRASTTSYIASMLRPEVIRDLRLEHHGLRMVPCEPGLQAVFEDGTVVPWWTD
                     HDRAVEEFTTSISVRDAESFDRVHRRLHGLARYLQPFFLEEPPNLYARGWAKVREGSR
                     AWRRFRSISGDELADLVRFTTGSLGEFVERHFESDRMRRMYLANNVYGMHAPPYMPGT
                     AIGLLFHLLSGGSEGVQGFYGHVMGGMGSITHAMAEAVRVAGGEIRTAALVSDVIVRD
                     GRATGVVLGSGEELTADIVLSNADPKRTFLDLVDPGELPEDFRDDVSAIKMAGPCAKV
                     NFVLSAEPTWTGMPADADANRRSLATLVPTLAEAQAIYDRHRDGEIPDRLWVDCVTAS
                     NVDDSLAPPGVHVMTAFVQYVPFALRRGTWDERRDELLSTVVGRIEESAPGFRGTIEA
                     AQVLTPLDLERTYGLTEGNIFHGDLHIGQLFSMRPTPSWSRYRTPVLGLYLCGAGAHP
                     GGGVTGAPGYGASHAALHDGRRRRRRRRRWH"
     gene            25152..25379
                     /locus_tag="VF195_01030"
     CDS             25152..25379
                     /locus_tag="VF195_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0046653 - tetrahydrofolate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX6579451.1"
                     /translation="MALKVPCPNCGWRPSTEFAWGGELRPIDAADAESDFARVFLPAN
                     AAGPQEERWFHLLGCRRWLTVTRDTTTNEIF"
     gene            25380..>27465
                     /locus_tag="VF195_01035"
     CDS             25380..>27465
                     /locus_tag="VF195_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6579452.1"
                     /translation="MAGTLQLGRKRIRVEDGDTVASALYRAGIRTFSRSLKYHRRRGL
                     YCGTGECPNCLVTVDGIPGIRSCVTPAADGMRVRRERGWPSVERDVLAVLDRLHVLLP
                     VGFYHKTFIRPRWSWAVASRMIRHAVGVGRLPGGSLVTRTNRHLHCDVLVVGAGESGR
                     AAASSAAVRGERVVLCDEGDVAEHPEGVEVLARHAAIGVYEGPMVALASAEGLVQVHP
                     RRVVTATGGSEVHPIFPGNDLPGVMLGRAAVGLAERGVAPGRRAVVVAAHDEGIGHLV
                     VLRDAGVPIAAVVVPSVLAEQVPGDVRTLIDATVVRAEGKDRVRFAVLRDVDGVTRGI
                     GCDLFVLSVGLSPRDDLVRMSAAGEVEPVGDAGAETSLTAEMPAAGTVCLCEDVSVGD
                     LERAWAEGFRSSELLKRYTTATMGPCQGAMCGRHLAAFAAAKNIETGATTAARTTARP
                     PSRPVPLAALAAAVHPVIEKRTSLHDVHVAAGARMEWSSSWLRPLHYGDAEEEYRAVR
                     ERVSVMDVGTLAKFLVTGPDATGLMDASFPARFEDLAPGRSRYLLALDEAGYVFDDGL
                     VCGLGEEGWYLTSTSGGADRMEAWLRDRADRLRLRAHVIDLTAERGAILVAGPLARTL
                     LGELTEAPLDTEAFPPLGVRELTVASVPCGAIRSGFVGEVAFELHHPRSRGPELWEAI
                     LRQGDGLGIRPHG"
BASE COUNT         4025 a         9546 c         9628 g         4266 t
ORIGIN      
        1 cttgtcgtgc tcgccgaacg tccggatcgc gaaggacttg agccactcgg gcgtcgcgtc
       61 gaccgcggcg ccacccacgg cgacgatcgg agaggaggcc gggccgaaga ccgcggagag
      121 gagctccgcc gttccgaggg ccacgcctgc ggcgagcacg ccgaccgtcg cccccacgcc
      181 acgacggatc tcggacgact cctgccccgc tcccacgccc acctgtgtcc ccatccaggc
      241 atgacgttac tcccgggccc gtgccggcct gttcgagacg gacctcgccg gataggctcc
      301 tccgatcgtc cccccgaagg gagaagtgga tgtcgcgacg cctgctcgcc ctggtcagct
      361 cactcggtct cgtgatcctc ttcttcaccc cgacggcccg agcggccccg gtcacggtga
      421 aggtcatgac cctgaacgtc ttctatggcg gtgacgagtg gaacctcgcg acgggccagt
      481 ggtgcgcgga tcctgccggc tgtcccgaga ccatggacca tgtcgtctcg acgatccggg
      541 ccgccgatgc cgacatcgtc ggcctccagg aaggcaccgc gaacgagtgc gtgatcgccg
      601 accgcctcga ttggcactgc gagccgcggc tccagctcat ctctcgctac ccgctccacg
      661 accctcccgg ggccgacggc gcgtacgtgc tcgccgaggt caccccgggt cggttcgtcg
      721 cgatctcgaa cgtccatctg ccgtccgatc cgtacggccc gtactgggtt cgcgacgggg
      781 agccacgcgc ggccgtgctc gacctcgagc gcacgacgcg ccttccggcg atccagcggg
      841 agctcgacgt gctccccgag ctcgccgacg ccggcatgcc ggtgttcctc accggtgact
      901 tcaactctcc atcccatctg gactggacgc cggcggtcga tgccgtccgc gaggaggtcc
      961 ggtacccggt ggtctggccg gtctcggcgg ctctcgccga ggcgggcttc cgtgattcct
     1021 accgcgaggt gcacccgaac cccgtggccg tccccgggtt cacctggacg ccacccggga
     1081 cgctcgagtc ggtgcccgac gaggtgcacg atcggatcga ctgggtgctg gtccagggtc
     1141 ccgccgagac gctcgccacg cagctcgtcg gcgaggccgg cggaccggac gtcgacatcg
     1201 agcgggatcc ctatcccacc gaccaccgtg gcgtggtctc gacgttccgc gtggaagccg
     1261 ccgatcccgc gctgttcgcc gcggtgggtg agcgcagggt gttcatcggc cgtgacctgg
     1321 atgtacgtgc ccacgggccg ggttcgcgcg tggtgctggt tcgcgcgggc cagggcccgg
     1381 cggcggcgct cgtctcacgg cccctcggcg gtgcgtccga cgtcaccctc acgttcccga
     1441 cgggaacctt gtcgcccggc gcgcacgagg cgctgctcgt gaacgcgagc gacgccgtgc
     1501 tgtcccggag cccgttctgg gtctatgcgg ccgggacccc accgagcctg cggacctcga
     1561 aggaggtcta cgaggtcggg gagccgatcc gcgtgcggtg gcgggccgcg cccgggtatc
     1621 gcttcgactg gctcggcgtg ttctcgcccg ggcgaggacc ggcgcagaac gcggaggact
     1681 gtaacgcggg gacctgcggc aacggccact acctcctcta tgactacacg gagactctga
     1741 tccaggggtc cacgctgatc ggccccgatt ccttcgccgg gtaccgcacg tggccgttga
     1801 aggtggggaa ctacgagatc cgcctcctca tggacgacgg ctaccgcctc ctcgggattt
     1861 ccaacagatt caagatcgtg cacgcctcgg cgtagcggcc gctcgcgcgg cggcgctcgc
     1921 gaccgacgag gtcgcgtggc caccgcgcgg gtggccggcg ggttacggtg ggcggcgaga
     1981 tgtctcccat cgtgatcatc gtgctcgtcg tggtcgcgct gatcgcggtc gcgtatttcg
     2041 ccggccttcg acccgcaccc gtcagcggtc gccggccgac gcccgggctc ctgcgccgcc
     2101 gctccaagtg gcggaaggtc cttcccttcc tgccgctcct cgcggccgtc ggctgcctgg
     2161 tctatgcgtt cagcgggttc cgtctcgccg tcaaggaagc ctccccggtg atcgtgctgg
     2221 tgctcgacgt ctccgattcg atgaacgcca ccgatgtcgc tcccgatcga ctctcggccg
     2281 ccgagaccgc cgcgatcgcc ttcctcgacg agctcccccc cgagttccgg gtcggactga
     2341 cgacgttcgc cggcgcggca cagctcgtcg tgagccccac gcgggtccgc gaggaggtgg
     2401 tgaacgcgct ggcggacctc accacgtcga agggcaccgt gatcggcgac gggctcaccg
     2461 tggcgctgga tgcgatcgaa gagctccggt ccgaggcccc ggacacgtcg gccgccgccc
     2521 tcctcctgtc cgacggcaac gacaccggga gccgggtcac gcccggccag gctgcgacgc
     2581 gggccgcttc cctcgaggtc ccggtgttca ccatcgtcgt cggccgggtg acggaaggcg
     2641 acggtcacgg cgccgacctg agtgcgctcg aggcgatcgc gaacaccagc ggcggcgaca
     2701 cgttcacggc agagacgagc aacgagctca cctcgatcta caccgacctc gggtccgccc
     2761 tgtccgtgga cctcgaggtc gcgccgtcga ccaccccctt cgtggtcgcc gcgatcgcgc
     2821 tcaccgtgct ggccggcctc atgctcgtct acctgccgcg ctgaaagcct acccaagggc
     2881 acgcggagcc gcgggaaccg tccacccccc actgtttcgc cggacgaagt cccgggaatc
     2941 agccttccgg agtctccgac aggaggaggg agccatgggt cagcaggtga aggagatcat
     3001 gaccgcacag ccggtcaccc tcgacaggga cgcgaccatc gccgaagcgg cgaagttgat
     3061 gcgcgatcgg ggcataggag acgtgatcgt ggtcgacggg gaggacgcgc aggggatcgt
     3121 cacggatcga gacatcgtga tccgcggggt cgccgagggc gccgatcccg acacgacccg
     3181 tctaggccaa gtcgtgagcg gcgacctgac gtcggtagcc cccgacgacc cggtcgagcg
     3241 ggcgatcgag ctggtgcgcg ggaaggcgat ccgtcgtgtc gctgtgctcg atggcgggaa
     3301 gcccgtcggg atcatctcga tcggcgacct cgcgatcgag cgggatccgc ggtcggcgct
     3361 cgccgacatc agcgaggagc cgccgggcaa gtaaccgcga tggacggcac ccgaacgacg
     3421 acgagggaca tcgagtccac ccgcagggag atgcatccgg agcggccgcc ggagaccggc
     3481 ctgctcctcg cgctcgacga ggcggtgggc gcgcttcgcc gggcacaacc cccgttcctc
     3541 ctgatgggcg gcctcgccac ctcgatcctc ggcgtggccg ccgcgtcacg gacatcgatc
     3601 tgttcgttca cgaccgtgac gtcgcgcaga tcctcgcggt cctgaaggcg gcgggcttcg
     3661 agacgatggt cgtcgccccg aactggctcg cgaaggggtt caaggacggg gtcctcgtcg
     3721 acgtgatctc gcgatcgacc catgacatcg tcctctcgga ggagatcctc gaacacgcga
     3781 tcgagatcga cgtggagggt cgacgcctcc cgtgcgtggc gccggaggag ctgatcgtga
     3841 tgaaggcgat cgccaccacg gaggacgcga cccgctattg gtacgacgcg ctcggtctcc
     3901 tcggtcgccc cgatctggac tgggagtacc tggttcgccg ggcgaagcag cacggtgctc
     3961 ggcgggtcgc cgcgctcctc ttcttcgccc agtccattga tctcctctct tcgccagtcc
     4021 attgatctcc tcgtcccgga tgacgtcgtc gaccagctga tcgatgcggt ccgacccgcc
     4081 gccggtccaa ccgatggatg atcctcgcta cgagcaggaa cgcatccggc aagccctcgc
     4141 gacggatgcg cgggtgggtg agccggagct cggggtggat ctcgtggcgg gccgggtgct
     4201 cgtgaccggt tccgtgccca acgagcgggt gcgagcagcg atcgacctgg tggtagccga
     4261 gatcgctcca ggggcggcgc tggacaaccg ggtcaccgtc cccccggcgg acgaaccgac
     4321 cggtgaggag acgatcggat gatccggctc gcggccgtcg gcgacgttca ctgcggaacg
     4381 gacatggggg cgcagatccg atcgcagttc gcctccgtgc ccgaccacgc cgacgcgctc
     4441 ctcctggccg gggacctcac ccgcctcggc ctgccggagg aggccaaggt cttcgcagac
     4501 gcgctccgag agctctcgga catgcccgtc gccgccgtgc tcggcaatca cgatcatcag
     4561 agcgaccgcc agaacgaggt tcgcgacatc ctcgaggacg cgggcgtccg ggtgctcgag
     4621 ggctcgtcgg tcgtctggca cgtgggcggc gctcagatcg cgatcgcggg cacgaagggg
     4681 ttcggagggg attcgaaggg acccatgcac cggcgttcgg tgagcgggag atgaaggcct
     4741 acgccgagca cacgcgggac ctcgccgacc gactcgaccg gtgcctcggt gaagtggtgg
     4801 aggccgacct caccgttctc ctcctgcact acgcgcccat ccgagacacg ttggccgggg
     4861 agccgcggga gatcttcccg ttcctcggca gctacctgtt cgcggagctg gctgatcggc
     4921 accgggtcga cctcgtcctg cacggccacg cacaccgagg gaccgagcgt ggcaggacgc
     4981 ccggcgggat cccggtccgc aacgtggcgc aggcggtgat ccagcagggc tacgcggtct
     5041 acaagctgga ccaggcccga ggcgggcggg tgttcggagc cgcgtgagcg cccatctggg
     5101 ccggcagggg taggaacctc accgctcggg tatctcttcg gcgatgcagg atctgacgct
     5161 cgaacggatg gcggatgtgc gcgcggaggc gctcccgtcc gcgcggggac cgctcagcgc
     5221 gcggatgctc gatctcctca ccgaccgaag cgtcgggcgg cacccggcgg gggtcccgga
     5281 cgacccattg ggggacgacg acctccatct ggcgctcggg ctcgcctacg agctctccta
     5341 tcgaggcctg cccggcgtgc gtgacgacct cgaatgggat cttggggtcc tcagcgcgcg
     5401 tcgcgacctc gaggatgcgt tcgaggccgg gttgcgccac acgatcggga gggagtccgt
     5461 ggatccgcgc gacgtccccg atcgcctccg ggaggtcgcc acgccaccgg atgccgactc
     5521 gctctccgcg ttcgtcgagc gagaagcgac cctcgaccaa gcccgcgagt tcttgatgca
     5581 tcgctcggcc taccagctca aggaggcgga tccgcactcc tgggtgatcc cgcgtctcgt
     5641 cggagcggcg aagtcggccg tcatggagat ccagtacgac gagtacggca gcggggacgc
     5701 cgcgtggatg cattccgagc tcttccgcac cacgatgatc gcggccggtc tcgacggttc
     5761 ctacggggtg tacctcgacc ggctccccgg cccgacgctc gcgaccgtga acctgatcac
     5821 gctgttcggg atgcaccgcc gatggcgcgg agccctcgtg ggccacctcg cggccttcga
     5881 gatgacctcg agcctgccga accgcgccta cgcgcagggg ttccgacggc tcgggttcga
     5941 cgacgacgcg gtgcggttct tcgacgagca cgtcgaggcc gactctgtcc acgaggtcct
     6001 cgcaggcgat ctcgccgccg gccttgcggc ggcggagccc gagctcgcga gcgacgtcgt
     6061 cttcggagct gtcgcgatgg cgctgctcga aggtcgcttc gcggacggtc tcctgcgagc
     6121 gtggcgggcc ggcgactcgg cgctccttgc tgtcgccttc cggtcgtcgt gagcgaggat
     6181 ctctcctacg tcctgccgta ccggagccgt ccgggagacg ggaacgagga tgaactggat
     6241 cggtatctcc gatggctgtc cgggagggtc caggtgatcg tcgtcgacgg ctcggagccc
     6301 gaccgtgtcc gtgcgcaccg ggagcgatgg gcccggatcg ccacccatgc cgtgcccgat
     6361 gcagacctct catgccggaa cgggaaggtc cgaggggtcc tcacgggact gcgccgagct
     6421 cagtacgagc acgtgatcat cgccgacgac gacgttcgct acgacgacga ggccctgctc
     6481 cggatgcagg agctcctgcg gaacgccgac ctcgtccgtc cacagaacgt cttcgacccg
     6541 ctcccgtggc acgcccgctg ggacacggcg cggatgctgc tcaaccgggc gctcgggaac
     6601 gatcacccag ggaccctggg cgtgcggcgc tccttcctcg catcgatcgg tggctacgac
     6661 ggcgacgtgc tcttcgagaa cctcgagctg gccaggacgg tgctcgcggc gggaggacgg
     6721 gtcgtcgacg cgcccgggat gtacgtgcgc agacttccac ccacccccgc tcgattcatg
     6781 gaacagcgcc ctcgccaggc ctacgacgac ctggcgcaac ccgcccggct ggggttcttc
     6841 ctggtgctcg ggcccgcgct ggcggtggtg gcgcggcgtc gacctcaggt tctcctcgca
     6901 gcggccatcg cctcgatcct cgtggcgcag gtcggccgga tccggcacgg cggcaaccgg
     6961 gtcttcgacc gggtctcccc gttcttcgct cccgtctgga tcgtcgagcg ggcttgcctg
     7021 gtctggctgg cggtcttccg gaggttctcg cggggaggct gtacgtacgc cggcgacgtc
     7081 atccggcgag cggcgactcc cccacgggtc cttcggcgga ggctgcgggg acggctcggg
     7141 ggctgaacgg cacgatcttg tgggtgccgt cgcagaacgg ctgcgtggcc gagcggccgc
     7201 accgacagat cgcgttcacg cggcgccgga tccggacctc gtctccccgc tcgtcgacga
     7261 ggcggatcgg ccctcgtacg agtaacggac cgtccgccgt gacacggacg acgcccacct
     7321 ccgctctacg gctcacgcgc tgcgattgcg agcggtcgcc cgtccgccct tcctcccggc
     7381 cgctttcttc tgggcggtcg tcccgccctg gctcgtcgtc cggcggcgcg tctccgagcc
     7441 ggacgccttc ccgccgcgcg ccgatgctcc gggtgagttg gcgatgcgcg ctgcccgctc
     7501 cttcgacatg cccttcttct tcagggcctc gtactggtcc tcgttcttca cgctcggcct
     7561 cttgtccggc atgacacgcc ctcctcgatc ggtgtcctcg gatatacctt ccccgaaccc
     7621 gcccccagta ctcacgcccc ccgcgcctgc atctcgcgct ccacgccgat ctgatcctcg
     7681 atgcagaacc gggtggcggg tcgcgcggcc agcctctcgg ggccgatcgc cttcccgcac
     7741 gcctcgcacg tcccgtacgt gccgttcgac agacgggcct ccgcatggtc gatgtcggtc
     7801 agctgtgcgt cgatctcgct gatgatcgag agatcccggg cacgttcgaa catctccgat
     7861 cctgcgtcgg cgacgtggcc gccggcggag gggtcgccgg ttggatcgcg ctccacgtcc
     7921 tccgcgactt ccgtcaggct cgatcggatt tcgttgaggc gggcgcgctc ccgctcgagc
     7981 agcttcgcgg tgcggttcga ctcggacatg gtccctccca tcggtgtggc tcgtggtgcc
     8041 accattccca tgtggccgcc ggtgcatgcg tcggatcggt catcgctcgt cgacgcgccg
     8101 gtcaggagcg gccgttccga ctcggccgtt cggctgaagg gcggtccgtc ccaagggatg
     8161 accgttcgac cgattgacgg gtgacggcgc tcccggcacg ctcagcccgt cccgggaagg
     8221 gggttccctg aaagcgcgac gtgtcgtgat cggtctcacc atggtgtcgc tgctggccgt
     8281 gccgttcgcg ccggcaggag ccgccccacg ctcgcaagac cctcaggtcg ggggcgctcg
     8341 cctcaccgac tcggatggcg accacctgga tgatgcgctc gagcgacgcc tccgccggtc
     8401 cgagcggacg acgcgccacg ccgtcatcgt ggcaacggat gggtcgctcg acctcgccgg
     8461 cgcccaccgg gtggcgggtg cgttctccat cggtcgcagg ttggagatcg tagggggctt
     8521 ctcggccaag ctcaccgccg ggcagatccg ccggctgggg tccacgcccg gcgtcctccg
     8581 gatcgaccac gacgcggtca tcagggccac gatggacgct gcgagggcgg actacggcgt
     8641 cgagtcggcc cgggcggatc tcggactgac gggtgccggc agcacggtct gcatcctcga
     8701 cacgggcgcc gatcccgacc acgagcagct cgactcgaag agcatcgtct ggaaggactt
     8761 cgtggggacc gccgcgaccc cggtcgacga ccacgggcac gggacccacg tcgcctcgat
     8821 cgccgtcggc gacggcgtcg gtggccccga tgcggccagg ttcggtggcg tcgctcccgc
     8881 ggccggactg tgggtcggca aggttctcaa cgcccagggg tcggggagcg agtccgggat
     8941 catcgacgcg atcgactggt gcgtcgacgc accggaggtc gatgtgatct cgatgagtct
     9001 cggctcggag ctgccgtcgg acggctccga cgttctcggc atcgcggcga acaacgccgt
     9061 ggcgagcggc aagatcgtgg tggccgcagc ggggaacggg ggcgatgcac ccgattccat
     9121 ggcctcgccc ggcgccgcct ccgacgtgat cgccgtcggc gctgcctcgt cctggtcggc
     9181 ggcgcccggc gtcgtcaacc gttcgaacgg tatctacctc gctcccttct ccgcccgcgg
     9241 cggtccgacg ttcgcggcgg acccgaagcc cgacgtggtc gctcccgggg tgaacgtgag
     9301 agcggcggat gcgaacaccg gatccggcta cgcgaccctc agcgggacgt cgatggcgac
     9361 cccgttcgcc gcggggtcca tcgcgctggc actcgaggcc tggaccggat cggaaccgac
     9421 gcccgccgac gtacagagcg cgatcgaggg aacggccgag gatttcggac cggcagggaa
     9481 ggatgccgac tggggcgcgg gcctgatcga tgtgctggcg ctggcctccc aggcggcagg
     9541 ggcctcggga gcgacggact tcccgaccca cgagcatctg tccggctcgg ttccggacag
     9601 cggcgagtgg acgcacgcgt tcgaggtcgg cccggacgac ctcgatgtgc cgatcgccgc
     9661 ctcgatcgtg ctcgacggat cgtgcgagga cttcctcggg ttcggctgtc tcttcggctg
     9721 ggcccccgac atcgacgcgg agctcctgga tcccactggc gagctgctcg acgtcagcgt
     9781 ctgcgcggcg atcgaggcgg gcgatccctc cccaggaccc tgctcgatcg ggcgtcaaga
     9841 aacgctgcac gcgatgccga cggtcgcggg gacgtacctg atccggttgt tcccgttcgg
     9901 cggctcaccg aacgacggac tcggtggagc gttcgacctg gacctgtcga ctggcccggt
     9961 cggcgacgac accacgccgc caccgccacc gccaccgcca ccgccaccgc caccctcgat
    10021 ccatgtcggt gatctcgatg acgtgtcgat caccctgtcg tccacacgat ggcgtgccag
    10081 ggcgaccatc cgcgtgcacg acggatcgca cgcggcggta tccggtgtcc tcgtacaggg
    10141 gcggttcggg ccgaacgggt ccaccctctc gtgcacgacc ggggctacgg gagcctgcac
    10201 gctgaagcgg gatctcaagc gcacccgtct gagcatcgag ttcgtggttt cagggctctc
    10261 gaaggaggca cacacctacc tcgccgcgag caaccacgat gcggacggag atagcagcgg
    10321 caccaagatc gtcgtgaccc ggccgtagga cggccgggtc acggatcgaa ggttccgccg
    10381 gggcgcgggc ccagctagtc cgtgtgcgag tgcgtcgcgc gttcgtcggt gtccgtgtcg
    10441 gtcgacgcgg tggccgcggc gaccgtgcgc ttggatggga agaacccgcc gatcagcgcc
    10501 aggatgccgc ccgccagcac gaaccacagg cccatcccca cgtcctggag ggtgtcgagc
    10561 gatccctcac gacccatctg gatcacgatc agggtgaaag ccacgagcgc gacggcaccg
    10621 gcgaatctcg tgagccagcc accacggaac gcgagaccca agatgatgac ggcaccgcag
    10681 atcgcggtga ccagtcccgc cgaggagaag aagccggccg tccccgcatc ggtggaccag
    10741 aaggcctgca cggcgacttc cgatccgtcg gtgccgccgg cccactcgaa gaagcttccg
    10801 accaacagga gcaccgccgc agcgacggtg aggagcacgc ggagcgcgag ccacggagaa
    10861 ggagcctcac tgaccgctcg cgtctccgtc gaggtcgcat ccgcgtcgtg atgctgatgg
    10921 gtcgcacgca tatctggccc tccttctggg agcgctggtc acgcgtggcg ggtcgcatcc
    10981 ggccgcccac gtctggcttc gggtataccc gggcagggcg cgcttcaatc ccctccgtga
    11041 gtcggcgtcg gaggtggacg gcgctcggac cgctcagttc gtgaggggga cgcggatgcg
    11101 gacgcgggtg tcggacacgt cggcgtccgg gaacagcgcc cggatcaacg cgaagcggcc
    11161 ggtccagcct tcgaggtcgg cgctctcctg ctccaccgcg acgtcttctg cccgcagctc
    11221 catcccgacc tcgtcggtcg agagctcgaa cctgatgtcc ctgttccccg cctcgaccgc
    11281 cccggagaac agctcggagg cggcgagccg gaggtcgtcc ctcacgggct cggggacttc
    11341 ccactgctcg gtcaccgagc caacgaagat ccgcaccacg agcgccagcg acggatcgcc
    11401 agcgacgctc aaccgggagg tggacaccct ggtcggcccc atcagtcctg ggagatgtcc
    11461 tcgatgctct gatagagcgt cagtacgcga tcgagtccgg tgagatccat cagacgacgg
    11521 agacccgatc cttccggcgc gacgagcgcg aggtcgccgc cgcgctcccg gatgtgcttg
    11581 aggcaggcga cgatcgctcc caagcccgac gagtcgatga agccgagacc gcggaagtcc
    11641 acgatgacct tggggtcctc ggcgtcgccc gcttccagga cggattcacg gaagctgggg
    11701 gccgtgtaga gatcgagctc acccttcacc tcgacaacgg cccagcgtcc atgggtgcgc
    11761 cggtcgaggg tcaggtccac ggtgccaacc tcctgatgtc ggggagccga tacggccccg
    11821 gtagtatagg tcgacaccgt gaagaatctt ccgacggcag tctgcgtgga cgtccctgcc
    11881 accgcggtgt tcatccaggt gctccgcaac gtcgttgcgg gagtggcggc gcgactcgac
    11941 ctgccgatcg acgagatcga ggacacgcgg ctcgcggtga ccgaggcggc gtcgctcctg
    12001 ctcgaggaag gcgacgcgac gtcgctcaag atgtcgatcg gtcgcgacgg cgacacgatg
    12061 gaggtcaccc tcacgtccga cgggcggtcg gatccgtggc caaccgatcg cgtcacggcg
    12121 tcgtggccct ggctcgtgat gaagggactc accgacgagg tccgagccga ccgcgccgag
    12181 ggcgccggtc cctcgatcgg cttcaccaag cgtcgcgtgc gtgtggatcg gtgagcgtgc
    12241 gtaccggcca gacacgatcc gacgaggaga tcatggagct cttccgtcga ttgcccgacc
    12301 cggccgcccg ggaggcgctc gccacggagt tcctgccgct cgccgagtac ttcgctcggc
    12361 gcttctcggg caggggtgag cccgtcgacg atctcaccca gaccgcctct ctcggcctgt
    12421 tgaacgcgat cgaccggttc gatcccgagc ggggggtgcc attctcgacc tacgcggcgg
    12481 ccacgatggt gggtgagctg aagcggcact tccgcgatcg cggatgggcg ctccgtgtcc
    12541 ctcggaacgt ccaggagacc gcgatcctcg tcaatcgcac cgtctcctcg atgtggcagg
    12601 acctgggccg agctccgacc gtcgcggaga tcgcgcgggc ggcggacatc ggagaagacg
    12661 acgtcctgca ggccctcgat gcgctgcagg cgtacacgac ggactcgctg gatgctccag
    12721 tcggcgacgc gtccaccacc accgccgagt cgatcgggag cgtggacccg agcttccagg
    12781 tctctgatga ctggctgagc atcgcgcccg cgctccgcga tctccccgag cgggagcgga
    12841 agatcctcta ccttcggttc ttcgagggga ggacccagac ggagatcgcc ggggagctcg
    12901 ggatctccca gatgcacgtc tcccggctcg tctcccaatc gctggagaag ctccggaagc
    12961 tcgctgaaga ggtgtgacgc gggagccgga cggagggggc ccgcgaccgg ctcccacccc
    13021 accatcgagc ggagcggtta cgacgtctcc cgcgcctcct tcacccgtgc gggcgtaccg
    13081 atctcgggtc cggcgttcgt gtccacggtg gccatcgacg tcgagatcga cgcgagcgtc
    13141 tctcccacgc tccgcatcga tgccgatccg atcgccacgt cacccaggga caacatgccg
    13201 acgagccggc cgtccttgac gacggggagc cgtctgatcc gccgctcggc catcagcgcc
    13261 ctggcgtcgc agagctgtgt gtccggcgat gtggtctcga tccccttcgt cgtcgcgatc
    13321 tctcccaagc gggcggtctt cgggtctttc cgttccgcga gcacccggag cgccagatcg
    13381 cggtccgtga cgatcccgat gagccggtcg ccgtcggtga tgggcacgac gccgatgtcg
    13441 ttcgcggcca tcaggccggc ggcgtcgccc gcgttcatgt cgggtgacgc ggtcacggga
    13501 gacatcgcca tcgcgtgtct gacgagcatc atgggaccac gtcctctctg ttgcccgggt
    13561 tccagcttct ccgcgcctcc cagcgttccc gccgacgccg tcgaccaaac gcggcaccat
    13621 cccgtgggtg gggtaccgtg gccccgtgac cgaggagcgg cagaaccgac gagcgggctc
    13681 ccaagcggat cgcggagcgg cggccaccac ggaccccgac gagtcaccca aggagcgcgt
    13741 tgaccgcgag ctgatcgagc tgttgaacgg cctccgtgtc atcctgccgg gcgtgcaggt
    13801 tctgctcgcc ttcctcctca ccgttccctt ctcgagcggg ttcgaggacg cgacctcgac
    13861 cgacaagatc gtgtacttcg ccgcggtcct gttgacggta ggcgcgacgg gggcgctcac
    13921 gatgcccgct gcctaccatc ggatccgatt ccggcaaggg gacaaggagc ggatgctgcg
    13981 tctgtcgaac acgttcgcga tcgtcggcct cgcgctgctc acgctcgcga tgacgaccgt
    14041 ggtcttcctc atcacgaacg tgctctacgg cgtcgtcgcc gcgatccccg tgagcctcgt
    14101 cgtcctgttg gtgctgggct tatcctggtt cgccgtgccg gtcttccgtc gggtcgagga
    14161 cgcgggctga ccgcgaatcc gggatcgcgg catcgagatc caagggaggg agaccggtca
    14221 gtccggcgga tacgcgccgc gcacaccggg gccgtccggt tcgcgctcct cgtccacgac
    14281 gggttccgag aggggatgcg gcgtctcgga gggttcctgt cccggatcgg gcgactccaa
    14341 gccagcgaac gaacccacga tcgtgccgta gagcagtccg gcgatgaggc cggcgatggc
    14401 gcaggcggcg acggcgccgg cccgcccgtc gaacgcgaag atcccaatgg cgatcccgat
    14461 cgcgatcccc acgagggagc cgacgacgag acccgtcgag agcctgcgct tgaggcgagg
    14521 ctcgaagcga tcctcgtcgg cctgcgatcc gctcatgaga cggagaggcg ccgggcccgc
    14581 ccgaacggag gagcgggcgg gcccggatga cgcgggaagg gctgaccttc cgggaaaggg
    14641 tcgccgtgga gttccatcac gtgcccatcg ttcccgcggc gatgaccgga caaacgcgag
    14701 ggagaggcgc gagcttcgcg cctctccctc gttcgaccga acgcttcggt taggccttgc
    14761 tgggcaggtc gggaccggtg gtcacctcag tgacggtcgt cccgttgccg ttctcgggcg
    14821 tgagcgaacg cgcccatccc ttgagctgct cgtagcgagc gcggcccgcc ttggcgccga
    14881 gcacgtacgc acctcccgcg agcaccagcc acttgatgcg gccggggcgg cgggtcttcg
    14941 agagcgctgc gagggtctcc ttcgagcgat cctgcgcgtc gtcgaggtcc tgccggagct
    15001 cgcccaccgc ctctcgcagc ttgtcgttct ccttggagag cttctcgacc cgccggtcga
    15061 ggaaacgctc cttgaccatg gatttcgctt cgtggatcgt cgctggttgt ctcatctcca
    15121 gatccctcct cggggtcgtc gatagagaac ctgtacccac tcttcgccag ggctaccctc
    15181 gggctacaga cgcttcgcca cgaccagcgc ggcgtcgtcg cggagctcgc cccgaggatc
    15241 gagcagggtg cgttccaccg tcttgatgat cccggcggcc tcctcgccgg cagcggcgac
    15301 gagcgccgac cggagcatgg cctctccttc atcgggatgc tcgacggaga gctccgtgag
    15361 cccgtcggtg aagagcgtga tcgcgtcgcc ccggcccaga tccgcgtgcg cctcatagag
    15421 cgagatgtcc tcgaacagcc cgaggagcga accgggtctc cccacggcgt cgagcgaccc
    15481 gtcggcacga atcacgaggg gcgggggatg cccacccaga cacgcggtca cggcagcccg
    15541 atcgtcggag aacgtgagtc ggacgacgca cacggtgcag aagcgatccc ccatcagctc
    15601 gctctcgatc aggaagccgt tgaggagccc cagaacctcc ggaaggggcc tcgtatcgtc
    15661 ctcgcgtgcg agactccgga gggcgatccg ggtcatgccc atgatggcgg cggcctccgg
    15721 gcccttgccg cacacgtcgc cgatcgcgag gtaccagacc ttgccgtcgc cgaacacgtc
    15781 gtagaagtcg ccgcccacct cgtcaccgaa ccgcgagggc cggtacatcg tggcgatctc
    15841 gaagcccggc acgtgcggca gggacatcgg gaggagcgct cgctcgaggg ccgtcgccat
    15901 gcggtctcgc tggtcgaaca gccgagtccg gtcgatcgtg gccgcgagcg aatccgcgat
    15961 gctctggagg aagtcggcgt cgtcgtcctg gaacgccccc ggcgttgcgg acgaggcatg
    16021 caggaccccg accacgcggc cgctcacccg cacgggtacc ccgacgtagg agcgtcgccc
    16081 gcgcagcgcc ggactgatca catcgtcggg cgtcatctcg ccgatgacga gtggccggcc
    16141 ggtcgcggcg atgcggccgg acgcgccggc acccagcggg atccggacga cttcgctcgc
    16201 ttcctccgcc tccccgacct gccccttgaa ccggagctcg cggccgtcgt ccgacaacag
    16261 gaggaaggac gtgccgtcgg cgagcaccgc gtcccggacc accggaagga cgcgctcgac
    16321 gagctcgtcg aggccgctgg ccgcgttcgc ggcgtcgctc acggcctgga gcttccggag
    16381 ccgatcctct gcgtgctccg cggtcgcccg agcgtgtcgc tcgtcgtcgc tcgaccggat
    16441 gcggtccaac gcgatcgccg cctggtgggc gatcgagagg aggagacccc gttcctccgg
    16501 cggaaccatg tggtcgcgat ggaatccgag gctgagtgct ccgaggacct ctgtccccag
    16561 cgccaccggc actcctgcga acccgccgta ccgtgcggct ccccgttcga tcttccgaac
    16621 gatcgggaat cgagccagga gctcgtctcg ggtctcgatg aagatcggtc tgccggtccg
    16681 cacgatctcg ggacccggcg ccggtcggtc ggtcgcgtgc cgcttccagc ccgtgtcggc
    16741 aggctcgtcc agggcggagc cgacgaggac ccggaggatc ccttgatcgt cgccggcgcg
    16801 gatcgtcagt cctccgacat cggcccccaa cgcctcgatc gccgcgctga ccgtcgcgtt
    16861 cgcgacgtcc tccgggttcg tcgcgcgcgc cagcacctcc gtgaccgctt ggaggcggtg
    16921 gagtcggttg ccggcgaggc gctcacgcgc gatcgccgag gtgcgttccc cgttcgcttg
    16981 ccgaagcctc ccggccatca cgcccgagcc gaccgacgcg atgagccaga gaaagacgga
    17041 gaactgggat cctcgctcga aatccgtgac gagcggcacg aacacgaggc cgccgacgag
    17101 actgacgagc gcccccgcga tcgggccgca gacgatcgcg gtcgccacgg acaggagcgc
    17161 gccgaagcct cccgggacgc cttcgaagct gcccggactc ttctcggcga acacgaggag
    17221 gaccgccacc tgcgcgagga cgaaccccgg gatccatcgt gggttccaga tccatggagc
    17281 gagcggcccg ggcgagtgcg gcttccggac cgaccgccgc tccgcccacc gttcggtcgg
    17341 gtccatggac atcacgcacc cacgtcgatc tcgaaccaca cttcgctgcg atccctcgtg
    17401 atgatccccc accggtccgc gagccggtcc accaggtaga gaccccagcc agaactctcc
    17461 gacgccgtgg gcagcctggg ctccgcgacg aaccccgtgc ccggatccga gaccaccaca
    17521 cggacccgtg ccggatccac cttcaagcgg agctcgatct tcgggaggcc ctcccggggg
    17581 gcgtgacgga ccgcgttggt gacgagctcg ctcaccagga gcgtagcgtc ctccaggacc
    17641 ttcgtcgaga ccgccggccc gagctcggcg ttcacgacgg ctcgggcttc tgaggcggct
    17701 tcgggggcca gctcgaacgt cgcccgatgt tccatggccg acatggtagg ggttcgggcg
    17761 atccccgaag ggacgcggtg acggacccgg acgcggttgc gtcgttccgt ggaaggcagc
    17821 tcgtcgggga cgagccgaga gcagctactt gcggtggttc cggaggggcg tgacccgctc
    17881 catcggtcga tgcgaccagc atcgttcgcc gtcgttgtac acggagagga cggttcggca
    17941 gtcggccacc tcgcacaccc ggccctcgtc gaagcggggc accggaggcc cgtcttgcgg
    18001 tgtttccgtc cgcacggacg tcgggtcgag ctcgacggac agttgcatcg gctgcctcct
    18061 cgccggggtc gttgtttctg gcgggcgaga tacccgagaa ccaccccggc caaacgcatc
    18121 tcgttcacgt gtgggtcaca tgcggtccgg gtacggtcga agcacgcaag gatgcaggag
    18181 acagggacgg gaggtcgacg tgccgcagca agcgtggagc gagaagcgcg agcgccagta
    18241 cgaacacatc aaggaagggt tgcgcaagcg aggccggtcg gaggatacgg cggaggagat
    18301 cgccgcgcgg accgtcaaca aggagcgcgc gaggtcgggc gaagctcgga cggcgagtcg
    18361 cacgtcggtg gacgacctgt cctcgggtcg gcgtgggggg ttgcgttccc actccggacc
    18421 gggtggaagg acctaccggc agttgtacga ggaagcgaag gatcgtggga tcgacggtcg
    18481 ctcgaggatg tcgaaggcgc agctcgagcg actcctcgac cgtcgtcgct gacggacgcc
    18541 gcgaggagtc aacagaagcc ggaccacgac aaagccagaa cggagcgagg acacgaggag
    18601 agagaggtgg cccccatgat cgatccgatc cgagacgtcc gccgcgagcc cgagcccgag
    18661 ccgcagcctc cgcagccgcc ggaaccggga ccaccggttc cgccgcagcc ggttccccag
    18721 ccacagcccg acccgcaacc acagccggtt cctcctcccc aacccgatcc ctcgccgccc
    18781 ccgcccacgg gttgaatcac gggagaccgc cggttcggcc tgcgtaccgt cgtttgcggt
    18841 acgaacggtc ttgggtaggc atctcacgtg ctccttgacg tgagcttccg gctgcccccc
    18901 gatgcgcgat cgcccgccga ggctcggcga accctcgagg cactccgagt gtcgctggac
    18961 gacccggtcg tggacaacgc cgcgctgctc gtgagcgagg tcgtctccaa cagcgtccgg
    19021 cacgcgggtc tcggtgccgc cgacgcgatc gaggtccgag tccgcgggtc gagggcgatg
    19081 ctccacgtcg acgtgatcga tcccgggccc gggttcgagc cccaggtccg tccgcgcgag
    19141 gatgacggcg gatgggggtt gtggctcctg gatcaactcg ccacgcgttg gggggtcgag
    19201 cagggtgaca cgacgcgcgt gtggttcgac ctggctccgc cggtttccgc ggcctccgcc
    19261 tcgggtacct gagctccgag gagtatccga acaacgatcg ggaggtggca gcatgggcga
    19321 gaacatggac aaggccaagg gtcacgcgaa ggaagccgtc ggcgatctga ccgacaacga
    19381 gcggctggag cgcgaaggga agctcgatca ggcgagcggc gacgtgaagg gcgcggccga
    19441 ggacgccaag gacaaggtcg gcgaaggcgt ggacgcggtc cgcgaccgac tcaagaacga
    19501 cgactagcag cccggtcggt ttctgagcgg ggcgcccccc ggcggtacgc cggggggcgc
    19561 ttcgatttgc gcatcggaac cggtcgagtg agagtgagct cttcaccacc ggaggagggg
    19621 gcatgcccga gaacggggat catccggccg actacacggg cctacacgcg ttctgcttcg
    19681 tcgatcatgt ggagccgggg accaatcccg aagaggtcgt ccggagactg atcgccaagg
    19741 ggaagccgcc gatcatgtac gcctcgaccg tggtcggtga ctaccaggtc ttcgtccacg
    19801 cgagggtgag gagactcgct gacctccagg acttgctcga aggcgtcatc ttcgaggccg
    19861 gggcccgttg cacctgggcg atcgagtccc cgatccggcg cgcgggggcg aagagaaaat
    19921 cgccaggtct catcgcgctc acgcggctca cgatggagca cgggacggag aacgccgctc
    19981 gcaagaagct cgtgacgcct ccgctcagca ccggcttcgt cggtgcatcg aagatcacgg
    20041 gagcgcacgg catcttgctc cagatgaccg gctcctcgat catcgatgcg gaacggaaca
    20101 tccgtgccgc attacaggac gtcgaaggcg tgctcgggat gtcgacggcg ttcatcgacg
    20161 gcgatcgaac cctcgaacgc tacggggaga tcccgctcaa cgagatggag gacgaggagc
    20221 cggaggagcc ggacgcggcg gaaggagaat ggtcctgagt cgttcacgca cctccgctgc
    20281 gggcaccgcc gcgcgcttcg actcgtagcg ctcgagcgcg accccgaggg ccgcagcggc
    20341 ctcggcggtg cggccggccc cgtgaagtgc ctccccgagg gcgcgcgcgg cctcggcctg
    20401 gagcaggacg aagctggtcg tgtccgcgat cgacaccgcc tcctccgcca acgtcaccgc
    20461 gcccgtcgga tcacctcgtg cgacgctcgt tcgggaccgt acggctctcc acgagacctg
    20521 tacctcgatg tcgtcgggtg ggccttcgcg cgcgaccagc gtcgcgagga ggtcggcctc
    20581 gtcgaactgg ccgaggtcgc agagcacatc cgccaggtcg aaggcgatct gtagtgagtt
    20641 gttctcgtcg ccctgtgcgc ggaagaagtc gtacacggat cgcagcaact cttcggctcg
    20701 ttcgagcttc ccttcgcgac gggcgatctc cgcgtgggcg gctttcgaga tctcgagcca
    20761 gcgccgctgt ccgagatcct cgaaggtgtc ccgcgcgcgg tccgtcgcca ccttggcgtc
    20821 accctgtcgc ccgaccgcgg cgagcatcat ggcctcgtcc agcaggagca gcgtcctggc
    20881 caggaggtcg tcgtcgagct gtgcgcgcgt ctcgtccagt cgctccagcg cttcatcgag
    20941 tggaactcga cccttgtgga gcgcgtaggc gatcagcggt gccgcctccg ccgccgaacg
    21001 cctgcttccg ctcgcgcgcg cgtgcgcgag cgccagctgt gcggcctcga gcatcgcctc
    21061 ggcatcgcct tcgaaccaat ggatcgtcgc gaggtccgcg agcgcggctg cgagactcgc
    21121 ttcatcgccg gcgtcctcga accggcggac cgacctctca aggtcccccc ggagaacgtc
    21181 ggccggtgtc gctcccgggt cgaggaggaa gcggagcatc gtctgctgca gtcgtgcccg
    21241 ctccccgatg acgctgtcgt cgtccggcgc cagcgatgcc aggacctcgg cgacagggcc
    21301 ctgggaacgc tcgatctcgc cggcgaacag cagggcttcg tggaggtcca tcaggacgcc
    21361 ggcccgctcg gcgtgcatct caggcaggag ctccgcggcc cgctcgagga gcccggcggc
    21421 cgcacgcacg tcgccgcggc cggccgccgc gcggccggcc gcggcgaggt gcgtgcccgc
    21481 tcgctccgcc aggggctgcg ctcgaggatc cgtctcgctc aggtcacgga ccgcgtggtg
    21541 cgccttctcc aggtggtagc ccacctgctc gtccctcgcc tctcccgccg gttcgcgggc
    21601 gagccgctcg gccgccgccg tgtgcagccg gaccctgagg gacttgggga tcgcctcgta
    21661 ggcggcctcg cgcgtcagcg catgccggaa ccgataggcg tcgcccggca tctcggcatc
    21721 ggcaaccacg aaatcccgct gcgccagctc ccggagcgtg gcgtcgacgt cgatcgcgtc
    21781 ctccccatcg agcagcgctt ccagatgggc ccgcgcgaag gccaggccca ggacggcggc
    21841 gcgctcgagc acctgccgag ctgccggatc gagtcggtcg atccgcgccg cgagcagggc
    21901 cgagatcgtg ggcgggatcg cgatcctcga gaggtcacca ggggtcgcgt cgggggtgat
    21961 cgccccctct tcctcgagca tcgtgacgat ctcctcgagg aagaacggat tgccgtcggc
    22021 gacgtcggcg atcgtgtcct cgaagaccgg ggccgcggtg gacgcgagga ggtggcgcgc
    22081 gagctctcgg ctgggcgccg caccgagcgg ttccaaccgg agcatcacgg cgcccggcgt
    22141 tcgtccccac gtggggagca ggtcgaggag atccgggcgc gccatgcaca acagcaggat
    22201 cggtgccccc cgcgcccact ccgcgagatg ctcgaggaca tcgaggagcg cgggtgccgc
    22261 ccactgcaca tcgtcgaacg cgagcacgag tggcccgcgg cgcgccagcg tctcgaagca
    22321 gatgcggatc gaccagacgg tctcctcttg ggtcgtcgtt cccggtgcga gcccgagcgc
    22381 ccgcgcgatg ccgtctgcgg cgagggatcc ctcgggctgc tcgacgagca ggtcgcggat
    22441 cttctcgacg atgctgaccg ggtcgtcctc ggggccggac ccggcggctt gcgccaccac
    22501 gtcccgcagc ggctggagtg cactgccctc cccatagacg ggacagcgac tgcggagcac
    22561 gaccgcctgt ggatgctctc gaaggaactc ggcgaccagg cgggacttcc ccagtcccgc
    22621 gctgccgatg agggtcagcg tccggcagcg agacgaggcg acgtggctct cgtaggtctc
    22681 gtgcaggagg cggagctcgg cggtgcggtt gacgaacggc ggctctggtc gaggcagacc
    22741 ctctccggtc tcgaggacct tcaggaggcg gtgggccttc acggggccgt cgaagccccg
    22801 gagctcatcg agcaacacct cttcggtctc gatcgccttg cgggcgatcc gcctggtgac
    22861 gccgtcgagg aggatctcgt gcgtgccggc atgctgctcc aggcgggccg cgaggttgac
    22921 cgatgtgccg atcacgaacg gctcaccggt gctccggtcg ctcgccacga cgttgcccgt
    22981 cgcgatgccg gtgcggatcg cgagctccac gtcccacttg gaacggagat catcgttggc
    23041 ctcgtcgatc agcgagcgaa gatcccaggc ggcccggacc gcccgcaggg cgtcgtcctc
    23101 gttcgcacgg gggaacccga acacggccat gaccgcatcg ccgatgaact tctcgatgtg
    23161 ccccccgtgc cggccgaccg cggtccgtgc cgcatcgaag taaccgccga tcacccggtg
    23221 gagggattcg gggtcgagcc gatgcgtcag atcagtcgac cccgccaggt cggtgaacag
    23281 gaccgtgacc tgtcggcgag actccgccgg ctcggtcgca tcgatcgcgt gcccgcacgc
    23341 caggcagaat cgagcgtgct cggggttgtc ggcggagcac gagggacaga gttgcacggg
    23401 cgaagcctac ggcggcccga gacccgatgg gtcccaaggc ggcgtggacc cgggtcagcg
    23461 gatcggctgc gcgcgccggg ctctctccgt agagtccgct ggacggtccg gccgtggcca
    23521 cgccggatct cgggagggtg cgtggcgagc tacgacgcga tcgtgatcgg cggcggacac
    23581 aacgggctga ccgccgcgtt ctacctcgcc cgcgccggtc ttcggaccgt cgtgctggaa
    23641 cggcgggaga tcgtcggcgg cgcgtgcgtc acggaggaga tcgcgccggg ctgtcgtgcg
    23701 tcgaccacct cgtacatcgc gtcgatgctc cgtccggagg tgatccgcga tctccgactc
    23761 gagcaccacg ggctgcggat ggtcccgtgc gagccggggc tgcaggccgt cttcgaggac
    23821 gggacggtcg tcccgtggtg gacggatcac gaccgggcgg tggaggagtt cacgaccagc
    23881 atctccgtcc gcgacgcgga gtcgttcgac cgggtccatc gccggctcca tgggctggcc
    23941 cgctacctcc agccgttctt cctcgaagag ccgcccaacc tctatgcgcg gggctgggcg
    24001 aaggtacgtg aggggagccg cgcgtggcgg cggttccgat cgatcagcgg cgacgagctc
    24061 gccgatctgg tccgattcac gacgggctca ctcggtgagt tcgtcgagcg acacttcgag
    24121 tccgaccgca tgcgtcggat gtacctcgcg aacaacgtct acgggatgca cgcgcctccg
    24181 tatatgcccg ggacggcgat cgggctcctg ttccatctcc tgtcgggcgg cagcgagggg
    24241 gtgcaggggt tctatgggca cgtgatgggc gggatggggt cgatcaccca cgccatggct
    24301 gaggcggttc gcgtggcggg cggcgagatc cggacggccg ctctcgtgtc ggacgtgatc
    24361 gtgcgggacg gccgcgcgac cggggtggtg ctcgggagcg gtgaggagct caccgccgac
    24421 atcgtgctgt cgaacgccga tccgaagcgc acgttcctcg atctggtcga tccaggagag
    24481 ctccccgagg acttccgcga cgatgtgtcc gcgatcaaga tggccgggcc gtgcgcgaag
    24541 gtgaacttcg tcctctcggc cgagccgacg tggacgggca tgcccgcgga cgccgatgcc
    24601 aaccgccggt ccctcgccac gctcgtcccc acgctcgcgg aggcccaagc catctacgac
    24661 cgtcatcgcg acggcgagat ccctgaccgt ctctgggtgg actgtgtcac ggcttcgaac
    24721 gtcgatgatt cgctggcgcc accaggtgtg cacgtgatga cagcgttcgt gcagtacgtt
    24781 cctttcgcgc tccgccgggg aacgtgggac gagcgtcgcg acgagctcct gagcacggtg
    24841 gtcgggagga tcgaggagtc cgcacccggc ttccgcggaa cgatcgaggc cgcgcaggtg
    24901 ctcaccccgt tggatctgga gcgcacgtac gggctgaccg agggcaacat cttccacggc
    24961 gacctccaca tcgggcagct cttctcgatg cggcccaccc cgtcgtggtc gcgctaccgc
    25021 acgccggtcc taggcctcta tctgtgcggg gcgggcgccc atccgggagg tggtgtcacc
    25081 ggtgctccgg gctacggcgc atcccacgcg gcgctgcatg acggccggcg ccggagacga
    25141 cggaggcgtc ggtggcattg aaggtgccgt gcccgaactg cgggtggcgt ccctcgacgg
    25201 agttcgcgtg gggcggtgag ctccggccga tcgacgccgc cgacgcggag tcggacttcg
    25261 cgcgggtctt cctgcccgcg aacgccgccg gtccccagga ggagcgatgg ttccatctcc
    25321 tcggctgtcg gcgctggctc accgtgaccc gcgacacgac gacgaacgag atcttctaga
    25381 tggcgggcac gctgcagctg ggccggaagc ggatcagggt cgaggacgga gacacggtcg
    25441 cgtcggcgct gtatcgcgcc gggatcagga cgttctcgcg atcgctcaag taccaccggc
    25501 gccggggtct gtactgcggg acgggggagt gtcccaactg cctcgtgacc gtcgacggca
    25561 tccccggcat ccgttcgtgc gtgacgcccg ccgccgacgg gatgcgggtg cgccgcgagc
    25621 gcggatggcc ctcggtcgaa cgggacgtgc tcgcggtcct cgatcgactg cacgtgctcc
    25681 ttcccgtcgg cttctaccac aagacgttca tccgtccccg ctggtcgtgg gcggtggcgt
    25741 ctcggatgat ccggcacgcc gtgggggtgg gacgccttcc cggcgggtcg ctcgtgaccc
    25801 gtacgaaccg acatctgcac tgcgatgtgc tcgtcgtcgg cgcgggcgag tcgggccgcg
    25861 ccgccgcctc ctccgcggcg gtccgtggcg agcgtgtggt cctctgcgac gagggcgacg
    25921 tggccgagca tcccgaaggg gtcgaggtcc tcgcccgcca cgcggcgatc ggcgtctacg
    25981 agggaccgat ggtggcgctc gcgtcggcgg agggcctcgt ccaggtgcat cccaggcggg
    26041 tcgtgacggc gaccggcggc tcggaggtcc atccgatctt cccggggaac gatctgcccg
    26101 gggtgatgct cggtcgggcg gccgtggggc tcgcggagcg aggtgtcgcg ccgggacgtc
    26161 gggcggtggt cgtcgccgcc cacgacgagg ggatcgggca ccttgtcgtg ctcagggacg
    26221 ccggcgtccc gatcgcggcc gtggtcgtcc cctcggtcct cgcggagcag gtgccgggcg
    26281 acgtcaggac actgatcgat gcgacggtcg tgcgtgcgga ggggaaggac cgggttcgct
    26341 tcgcggtgct ccgggatgtg gacggggtga cccggggcat cggctgcgat ctgttcgtcc
    26401 tctcggtggg tctgtctccg agggacgacc tcgtccggat gagcgccgcg ggagaggtcg
    26461 agcccgtcgg cgacgccggg gcggaaacct cgctgacggc cgagatgccc gccgcgggga
    26521 cggtgtgcct gtgcgaggac gtgtccgtgg gggacctgga gcgcgcgtgg gcggaggggt
    26581 tccgctcgtc ggagctcctg aagcgctaca cgacggcgac gatggggccc tgccagggag
    26641 cgatgtgcgg gcggcacctg gcggcgttcg cggcggccaa gaacatcgag acgggcgcca
    26701 ccaccgccgc tcggaccacg gcgcgcccgc cctcccgccc ggtgcccttg gccgctctcg
    26761 ccgccgcggt ccatccggtg atcgagaagc gcacgtccct gcacgacgtg cacgtggccg
    26821 ccggcgcccg gatggagtgg tcaagctcct ggctccgccc tctgcactat ggtgacgccg
    26881 aggaggagta ccgcgccgtc cgcgagcggg tatccgtgat ggatgtcggt acgctcgcga
    26941 agttcctcgt caccggtccc gacgccacgg ggctgatgga cgcgtcgttc ccggcccgct
    27001 tcgaggacct ggctcccggg aggtctcgct acctgctcgc tctcgacgag gcgggctacg
    27061 tcttcgacga cgggctcgtc tgtgggctgg gcgaggaggg ctggtacctg acgtcgacgt
    27121 cgggcggcgc cgatcggatg gaggcgtggc tccgcgaccg cgccgatcgt ctgcggctgc
    27181 gtgcccacgt gatcgacctc acggccgagc gaggtgcgat cctcgtagcg gggccgctcg
    27241 cgcgaacgct cctcggggag ctcacggagg cgccgctcga cacggaggcg ttcccgccgc
    27301 tcggggtccg ggagctgacc gtggcgagcg ttccctgcgg cgcgatccgc agcggtttcg
    27361 tgggcgaggt tgcgttcgag ctccaccatc cgcgctcgcg gggtccggag ctctgggagg
    27421 cgatccttcg ccagggggac gggctcggga tccgccccca cggac
//
LOCUS       DASUYD010000017         7410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1333223, whole genome shotgun sequence.
ACCESSION   DASUYD010000017 DASUYD010000000
VERSION     DASUYD010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7410
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333223"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1112)
                     /locus_tag="VF195_01040"
     rRNA            complement(<1..1112)
                     /locus_tag="VF195_01040"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            complement(1445..2707)
                     /gene="tyrS"
                     /locus_tag="VF195_01045"
     CDS             complement(1445..2707)
                     /gene="tyrS"
                     /locus_tag="VF195_01045"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015760047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX6579453.1"
                     /translation="MAADVLTTLASRSVDLVPKGGLEERLAAGRPLRVKLGVDPSRPD
                     LTLGHAVVLRKLREFQDAGHIAVLIIGDFTARIGDPSGQSETRPMLTEEEIGVNAETY
                     FAQASKVIDVDAAEIHGNAEWLAELDTVEVIRLAATATLAQMLEREDFRERYQGERPI
                     SVVELLYPLLQGYDSVAIRADVELGGTDQTFNLLMGREVQRAYGQEPQVVLTLPLLEG
                     TDGIRKMSKSLDNYIGLTEPSDEMFGKLMRIPDGLITKYLRLSTPLDTTEVDDVERGL
                     AGGSVGPNDAKRRLAREVVDLYHGSGAGAAAEQRFDLVHRRRELPEDVPAVTVPAALF
                     GPADGAASVVYVPALLEALGLVSSRSEARRLQAQGGVRMDGEPMPAEDIRVEGPPPGQ
                     LAGSIWQVGRRKFARLEGVETEGGSSPR"
     gene            2769..5012
                     /locus_tag="VF195_01050"
     CDS             2769..5012
                     /locus_tag="VF195_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX6579454.1"
                     /translation="MTRRHFPLAAFTAACAVFSAACGVHLEDVPRRALAQTSFVYAAD
                     DSVITELHAVEDRVVLPSSQIPSWIRDAAVAIEDRRFYQHHGIDGRAILRASYVNLRD
                     GSIEEGGSTITQQLVKNLYTGGERTLARKLSEASLAWQLEDRLSKEEILTRYLNTVYF
                     GQGAYGVQAAARTFFGVDAIDLNLARSALLAGLITSPGHFDPYEYPSRAKGRRRVVLR
                     LMHEFGMITEAQRRHAGGAPIRLHPVTTFGSYDYPYFVDYLKEWFLTNPAFGDTREDR
                     YKLLFTGGLQIHTTLQPAVQSAAQRAVDSVLSYPDDPSAAVTVVDPRNGFVLAMVGGD
                     QHDYWRDRDAGRVNLATATGGTGRQSGSSFKPFALVAALEAGLSPSETFPAPSTLQLA
                     LETGEIWDVTNAEGAGYGAMTLRSATIRSVNTVYAQLIDRLGPATVVEVAERMGLRCC
                     RRVSNPRRPLQPYLSAVLGTNEVNTLEMATAYGTLATGGVRSRPVPVSRVTDARGTVL
                     WSANTTGDRVLDPQIASVANEILNEAVLYGTGTAANIGRPQIGKTGTAMDHNDAWFVG
                     AVPQMVAAVWVGFPQGRIRMEPPRTRITVFGGTWPAQIWRLLMLEATAGLPAVAFPNP
                     EVGFVSVSVDASQTPDCLPNPFTLPQNIETLQFIEGTEPTEMCTSPSSLQEVLVPSTV
                     GSSESIAIEGLTQAGFYVEVKTAPSTQPKGTVIAQVPSAGTEAFQTSTVTITVSSTVP
                     EGSVASP"
     gene            complement(4966..5601)
                     /locus_tag="VF195_01055"
     CDS             complement(4966..5601)
                     /locus_tag="VF195_01055"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX6579455.1"
                     /translation="MTQKRLSRSFYARAAHEVAPDLLGQVLVRIFPDGSRASVRIVEV
                     EAYGPDDPASHAFRGETPRNAVMFGPPGHLYVYFTYGIHHCMNVVTGETAEGSAVLLR
                     AAEPLEGEEEMVVRRGRARPLDLCSGPGRLAQALAVDRRDDGIDLVAGDGLWLEAASR
                     VERVAVGNRVGVHETSRQWRYWLDGSPFVSRGRPGPPTAKRRTPRARSMTP"
     gene            complement(5598..7322)
                     /gene="polX"
                     /locus_tag="VF195_01060"
     CDS             complement(5598..7322)
                     /gene="polX"
                     /locus_tag="VF195_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016577835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HEX6579456.1"
                     /translation="MPRANDAIEAMLIEYADLLSILFEDPYKPRAYEKAARAVGGHPV
                     DLEGKDLPEILKVPSVGRSIGEKIQELLTTGALAELEELRERIPPGVRGLMSIPGLGP
                     KKAMVLHQELGVGSVDELVAAIEQDRVAALPRFGAKTQENIARGIRQMRSAGGRVKID
                     VALGVAETFLAILRPLPAARRVAYAGSLRRMKETIGDVDLLVAGSEASPVMDAFVGHA
                     SVARVIAKGDTKCSIVTDVGLQIDLRVIPEEAWGAAMIYFTGSKSHNIRIREIAVRRG
                     LKLSEYGLFDAKSGDLLAAESEEDVYGRLEMQYIEPTLREDRGEVDAALAGELPAVIT
                     ERQLKGDLHTHTNLTDGLASLEEMVARGAELGYAYFAITDHAPNLYMQRMTEEKILAQ
                     RSRIEKLRAKYPKMTLLHGTELNVDPDGAVDWDGEFLAGFDLTVASVHSHFSQPRDEM
                     TTRVIRAIENPYVDIIGHLTTRQIGKRPPVDLDLEAVFEAAGRTGTAMEINSHPDRLD
                     LSDEHVLWARRHGVRFAVNTDAHAVPHLDHRRFGVGMAQRGWLTKDEVINAWPLRKLQ
                     RFLAAGRP"
BASE COUNT         1159 a         2631 c         2361 g         1259 t
ORIGIN      
        1 ccgctggcaa ctaaggacag gggttgcgct cgttgcgcca cttaaggcaa cacctcacgg
       61 cacgagctga cgacagccat gcaccacctg tgcaggctcc gaagagcgcc cgctttcacg
      121 ggtttcaccc tgcatgtcaa gcccaggtaa ggttcttcgc gttgcatcga attaagccgc
      181 atgctccgcc gcttgtgcgg gcccccgtca attcctttga gttttagcct tgcggccgta
      241 ctccccaggc ggggtgctta atgcgttagc ttcggcacgg acggggttga tatccgccca
      301 cacctagcac ccaacgttta cggctaggac taccagggta tctaatcctg ttcgctcccc
      361 tagctttcgc gcctgagcgt caggtaaggc ccagagatcc gccttcgcca ccggtgttcc
      421 tcctgatatc tgcgcatttc accgctacac caggaattcc gatctcccct acctacctct
      481 agcctgacag tatcgaatgc agtcccgggg ttgagccccg ggatttcaca cccgacttgc
      541 caagccgcct gcgcgctctt tacgcccaat gattccggac aacgctcgcc tcctacgtgt
      601 taccgcggct gctggcacgt agttagccga ggcttcttct gcaggtaccg tcacgagatc
      661 gcttcgtccc tgccgaaagg ggtttacgac ctgaaaggcc ttcgtccccc acgcggtgtc
      721 gctgcgtcag gctttcgccc attgcgcaag attccccact gctgcctccc gtaggagtct
      781 gggccgtgtc tcagtcccag tgtggctggt cgtcctctca gaccagctac ccgtcgtcgc
      841 cttggtaggc cgttacccca ccaacaagct gataggccgc gggcccatcc ccgaccggcg
      901 gacctttcct cttctgacca tgtggtcgga ggagaatatc cggtattagc tccggtttcc
      961 cggggttatc ccagggtcgg gggcaggttg cccacgtgtt acgcacccgt tcgccactga
     1021 tccccgggcc gaagcccggt tcaccgttcg acttgcatgt gttaggcgca ccgccagcgt
     1081 tcgtcctgag ccaggatcaa actctccgta gagatcgatc ggaaccgacg gatcggtccc
     1141 gttcgacttg atggaggttc ggccctccga gcgaggcact cgctcgcagg gcctgtcccc
     1201 gccagacgtc tccgtctgga cggatgaagc gtgccatcct ccccggggca cggcgaaccg
     1261 tgccgatgtg aacgatggca agcatcggct ctttgacacg ctgttcagtt ctcaaggtgc
     1321 ggccagacgc gaagtccctg gtcagagcgt tggacaccct cgggaaaccg cgccggacgg
     1381 gtaggatagc agtcccggtc tctccgtcaa ccgagctcat gggctcgaat cgccgttcgg
     1441 gaggttacct cggagaggaa cccccctccg tttcgacgcc ttcgagccgc gcgaacttcc
     1501 gacgaccgac ctgccagatc gacccggcca gctgacccgg cgggggcccc tccacccgga
     1561 tgtcttcggc tggcatgggc tctccgtcca tccgtacccc tccctgggcc tgcagccgcc
     1621 gggcctcgga ccggctcgaa accagaccca gcgcttcgag gagcgcgggt acgtagacga
     1681 ccgaggccgc gccgtcggcg ggtccgaaga gcgccgcggg cacggtcacc gcgggaacgt
     1741 cctcgggcag ctcgcgccga cggtggacca ggtcgaagcg ctgctcagcg gcggcccccg
     1801 caccggaccc gtggtacagg tcgacgacct cccgagcgag tcggcgcttc gcatcgttcg
     1861 ggcccaccga tccacccgcc aacccacgct cgacatcgtc gacctctgtc gtatcgagtg
     1921 gggtgctcag ccggaggtac ttggtgatca gcccatccgg gatccgcatc agcttgccga
     1981 acatctcgtc ggacggttcc gtgagcccga tgtagttgtc gagcgacttc gacatcttgc
     2041 ggatgccgtc ggtgccctcg agcagcggga gggtcagcac cacctgcggc tcctgcccgt
     2101 acgctcgctg tacctcacga cccatcagca ggttgaacgt ctggtcggtg ccgcccagct
     2161 ccacgtccgc tcggatcgca accgagtcgt agccttgcag gagcggatac aggagctcca
     2221 ccacactgat gggtcgctcc ccctggtagc gctcccggaa gtcctcgcgc tccagcatct
     2281 gcgcgagcgt cgcggtcgcg gccaaccgga tcacctccac cgtgtccagc tccgccagcc
     2341 actccgcgtt gccgtggatc tccgcggcgt ccacgtcgat gaccttgctg gcctgggcga
     2401 agtacgtctc cgcgttcacc ccgatctcct cctccgtgag catcggacgg gtctcggact
     2461 ggccggacgg atccccgatc cgagcggtga agtccccgat gatgagcacc gcgatatggc
     2521 ccgcgtcctg gaactcccgc agcttccgga gaacgacggc gtgtccaagc gtgaggtcgg
     2581 ggcgactggg atccacgccg agcttcactc gaagcggtcg gcccgccgcg agccgctcct
     2641 cgagaccgcc cttcgggacg aggtcgacgc ttcggctggc gagcgtcgtc agcacgtcgg
     2701 cggccatggc gcggcgatgc taccgcacct ccccgatccg ccccgggacg cgggtacact
     2761 cgcccgcgat gacccgccgg cacttccccc tggcggcctt cacggcagcc tgcgctgtgt
     2821 tctctgctgc gtgtggcgtc cacctggagg acgtccctcg acgcgccctc gcgcagacgt
     2881 cgttcgtgta cgcggccgac gacagcgtga tcacggagct gcacgccgtc gaggatcgcg
     2941 tggtcctccc ctcgtcacag atcccttctt ggatccggga tgcggcggtc gcgatcgagg
     3001 atcggcgctt ctaccaacac catgggatcg acggacgagc gatcctccgc gcgtcctacg
     3061 tgaacctccg agacgggagc atcgaggagg gcggctcgac gatcacgcag cagctggtga
     3121 agaacctcta cacggggggc gaacgcacgc tcgcccgcaa gctgagcgag gcttcgctcg
     3181 cctggcagct cgaggatcgc ctgtccaagg aggagatcct cacgcggtac ctgaacacgg
     3241 tgtacttcgg gcagggcgcc tacggtgtcc aagcggcggc ccggacgttc ttcggcgtcg
     3301 acgccatcga cctcaacctg gcccggtccg ccctgctcgc cggcctgatc acgagccccg
     3361 ggcacttcga cccgtacgag tatccgagcc gggccaaggg aaggcggcgc gtggtgctcc
     3421 gcctgatgca cgagttcggg atgatcacgg aagcgcaacg ccgtcacgcc ggcggagcgc
     3481 cgatccgtct ccaccccgtg acgacgttcg gcagctacga ctacccctac ttcgtcgact
     3541 acctgaagga gtggttcctg acgaaccccg ccttcggcga cacgcgggag gaccggtaca
     3601 agctcctgtt caccggcggc ctccagatcc acacgaccct ccagccggcc gtgcagtccg
     3661 ccgcccagcg cgccgtcgat tcggtgctct cctacccgga tgacccctcc gccgccgtga
     3721 ccgtcgtgga cccgcgcaac ggcttcgtgc tggcgatggt cggtggcgac caacacgact
     3781 actggcggga tcgtgacgct ggccgcgtca acctggcgac cgcgacgggc gggacgggac
     3841 ggcagtccgg ctcgtccttc aaaccgttcg cgttggtggc ggccctcgag gccggcctgt
     3901 cgccgtcgga gacgttcccc gccccctcga cgctccagct ggccctcgag accggcgaga
     3961 tctgggatgt cacgaacgcc gagggagccg gatacggagc gatgacgctc cgctcggcga
     4021 cgatccggtc ggtgaacacg gtgtacgcgc agctgatcga ccgactcggt cccgccaccg
     4081 tcgtggaagt ggctgagcgg atggggctcc gctgctgccg ccgcgtgagc aaccctcgcc
     4141 gcccgttgca gccgtacctc tcggcggtcc tcgggacgaa cgaggtcaac acgctcgaga
     4201 tggcaaccgc gtacggcacc ctggctacgg gtggcgttcg gtcccggccg gtccccgtgt
     4261 cccgcgtcac cgacgcacgg ggcaccgtgc tgtggtccgc gaacacgacc ggcgatcggg
     4321 tcctcgatcc gcagatcgcg tcggtggcca acgagatcct gaacgaggcc gtcctgtacg
     4381 gcacgggcac cgcggcgaac atcggtaggc cgcagatcgg caagaccggc accgcgatgg
     4441 accacaacga cgcctggttc gtgggcgcgg tgccgcagat ggtggcagcc gtgtgggtcg
     4501 ggttccctca gggacggatc cgcatggaac cgccgaggac gaggatcacc gtcttcgggg
     4561 gaacgtggcc cgcgcagatc tggcggctgc tgatgctgga ggcgaccgcc ggcctgcccg
     4621 ctgtggcgtt ccccaacccc gaggtggggt tcgtgtccgt ctccgtggat gcatcgcaga
     4681 cgcccgattg cctcccgaac ccgttcacgc tcccccagaa catcgagacc ctgcagttca
     4741 tcgagggaac ggaacccacg gagatgtgca cgtcaccctc gtcgctgcag gaggtgctcg
     4801 tcccgtccac ggtcggttcc tcggagtcga tcgccatcga gggcctgacc caggccggct
     4861 tctacgtcga ggtgaagacg gcgccctcga cgcaaccgaa gggcacggtg atcgcacagg
     4921 ttccctcggc gggcaccgag gccttccaga ccagcaccgt gacgatcacg gtgtcatcga
     4981 ccgtgcccga ggggtccgtc gcttcgccgt aggcggtccg ggccttccgc gagacacgaa
     5041 cggactcccg tcgagccagt agcgccactg gcgactcgtc tcgtgcacac ccacgcggtt
     5101 ccccacggcg acccgctcga cccgcgaggc tgcctcgagc cacaggccat cgcccgcgac
     5161 caggtcgatc ccgtcgtcgc gtcgatcgac cgcgagcgcc tgcgcgagcc ggcctggtcc
     5221 ggagcacaga tcgaggggtc tcgctcgacc ccgccggacg accatctctt cctcgccctc
     5281 gaggggctcg gcggcacgca ggagcaccgc gctcccctcc gcggtctctc cggtcacgac
     5341 gttcatgcag tgatggatgc cgtaggtgaa gtagacgtac agatgccccg gagggccgaa
     5401 catcacggcg ttccgcgggg tctcgccgcg gaacgcatgg ctcgcggggt cgtcaggccc
     5461 gtaagcctcc acctcgacga tccgcaccga ggcacgcgac ccgtcgggga agatgcggac
     5521 caggacctgg cccaggagat ccggcgcgac ctcgtgggcc gctctcgcat agaacgatcg
     5581 cgacaaccgc ttctgcgtca cgggcgaccc gcggcgagga agcgctggag cttccggagc
     5641 ggccaggcgt tgatgacttc gtccttggtg agccacccac gctgggccat tccgacgccg
     5701 aaccggcgat ggtccagatg cggcacggcg tgcgcgtcgg tgttcacggc gaaccgcacc
     5761 ccgtgtcgac gcgcccacag gacgtgctcg tcggagagat cgagccggtc ggggtgcgag
     5821 ttgatctcca tcgccgtgcc ggtccggccg gcggcttcga acaccgcctc gaggtcgagg
     5881 tcgacgggcg gtcgcttccc gatctgccgc gtcgtgagat ggccgatgat gtcgacgtag
     5941 ggattctcga tcgcacggat cacccgggtc gtcatctcgt cgcgcggttg cgagaagtgc
     6001 gagtgcacgg atgcgaccgt gagatcgaac cccgcgagga actccccatc ccaatccacg
     6061 gccccgtccg gatcgacgtt cagctccgtt ccgtggagga gggtcatctt cgggtacttc
     6121 gcccggagct tctcgatccg cgaccgctgc gcgaggatct tctcctccgt catccgttgc
     6181 atgtacaggt tgggcgcgtg gtcggtgatc gcgaagtagg cgtacccgag ctccgctccg
     6241 cgcgccacca tctcctccag gctcgccaag ccgtcggtca ggtttgtgtg cgtgtgcagg
     6301 tcgcccttca gctgccgttc cgtgatcacc gcgggcagct caccggcgag cgccgcatcg
     6361 acctcgccgc gatcctcgcg gagcgtgggc tcgatgtact gcatctcgag tcgcccgtag
     6421 acgtcctcct ccgactcggc cgcgaggagg tcacccgact tcgcatcgaa gagcccgtac
     6481 tcactcagct tgagaccacg gcgcacggcg atctctcgga tccggatgtt gtgcgacttg
     6541 gagccggtga agtagatcat cgcggctccc cacgcttcct ccgggatcac acgcaggtcg
     6601 atctggagcc cgacatcggt cacgatcgaa cacttcgtgt cgcccttcgc gatcacacgg
     6661 gcgaccgacg cgtggccgac gaacgcgtcc atcaccggcg atgcctccga gcccgcgacg
     6721 aggaggtcaa cgtcgccgat ggtctccttc atccggcgca acgatcccgc gtaggcgacg
     6781 cgtcgggccg ccggcagcgg acggaggatc gccaggaacg tctccgccac gcccagggcg
     6841 acgtcgatct tgacccgccc tccggcggat cgcatctgcc ggataccgcg ggcgatgttc
     6901 tcttgcgtct tggcgccgaa ccgtggaagc gccgcaacgc ggtcctgttc gatcgcggcc
     6961 acgagctcgt ccaccgagcc cacgccgagc tcctggtgga gcaccatggc cttcttcggg
     7021 ccgagcccgg ggatcgacat cagaccacgg acgccgggcg ggatccgctc ccggagctcc
     7081 tcgagctctg cgagcgcgcc ggtggtcaag agctcctgga tcttctcgcc gatcgagcgc
     7141 ccgaccgagg ggactttcag gatctcgggg aggtccttgc cctcgaggtc gaccgggtgg
     7201 ccgccgacgg cccgcgcggc cttctcatag gcccgcggct tgtaggggtc ctcgaacagg
     7261 atcgacagca ggtcggcgta ctcgatgagc atcgcctcga tggcgtcgtt cgcccggggc
     7321 atcgcctcag gctacgccga agaggcggag cagcagcgcc ttctgcgtgt ggagccggtt
     7381 ctcggcctgg tcccagacca cggaccgcgg
//
LOCUS       DASUYD010000018         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1113133, whole genome shotgun sequence.
ACCESSION   DASUYD010000018 DASUYD010000000
VERSION     DASUYD010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1113133"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VF195_01065"
     CDS             <1..1024
                     /locus_tag="VF195_01065"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020514081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX6579457.1"
                     /translation="GLHDAGLTDSARVVVEKPFGHDRESAIALAEEIHQYIDESQLYR
                     IDHYLGKMGLVEILHLRFANAMLEPVWSRNFLSSVQITMAESFGVEDRGHFYDPVGAL
                     RDVVVNHLMQVVAASAMEAPSRGDPSTIKDAQVNLFRAVAEADPAHYVRGQYEGYQAI
                     DGVAQDSTTETFAALRLDIESWRWAGVPFFIRTGKRLPITQTELRLVFKHPPKLGFGV
                     LDRRPDENQLVVRLDPTTGIRLTVEAHRADLEGAAPVHLDMEFATEGGEGPTPYEVLL
                     DAAMRGDSTRFTRQDGVEETWRVFQPLLDSPPPVHPYAPGTWGPDAANGLLEGFGTWH
                     EPWVSP"
     gene            1069..2916
                     /locus_tag="VF195_01070"
     CDS             1069..2916
                     /locus_tag="VF195_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005561489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6579458.1"
                     /translation="MPSPFPPIADYAFLSDCHTGALIAPDGAVDWLCVPRYDSPSIFG
                     SLLDRQAGAFRFGPYGINVPSEIAYEPGTNVLRTTWKTPSGWVVVRDALTMGARSGED
                     TVTPHTRPPADDDADHTLVRTVECIDGQVEVELVCEPIFDYGRVVASWTVEDGEGHTA
                     DAMGGDLTIRLQTDLAMGAEGDRVRARHVLNAGDRAFCSLSWGHDLTGPADVDEAEAR
                     IDATVRFWRRWLGRARIPDHRWREPIQRSALVVKGLTYMPTGATVAALTTSLPETPGG
                     ERNWDYRFTWMRDSTFTLQALHWLNLDWEADEFMQFVADVEPNDDGGMQIMYGIDGRR
                     DLTESTLDELSGYAGARPVRIGNGAFDQRQNDVYGAVLDSILMHTRRSQRLPRRLWPI
                     VQSQAECATRVWREPDQGIWEARGAPQQYVSSKLMCWVALDRAAKLAQIGGDSDLSQT
                     WGKTAEEIHQDILDHGMSDRGVLRQHYDTDALDASTLLAPLFGFLPGTDERMHASVLA
                     IADELTENGYVLRYKTDQTDDGMSGKEGTFLICSFWLVSGLAIVGEEQRARDLMERLL
                     RISSPLGLYAEEYDVETGQHLGNTPQAFSHLALIEAAGRIILGERVAEL"
     gene            2943..>3329
                     /locus_tag="VF195_01075"
     CDS             2943..>3329
                     /locus_tag="VF195_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEX6579459.1"
                     /translation="MSDYDVIIIGTGAGGGTLARHLAPSGKRILLLERGGWLAREPQN
                     WSTADVFIDGRYISPDTWYDAKGRTFQPQVHYFVGGATKLYGAALYRLREKDFGELKY
                     PDGISPAWPIGYSDMEQYYTLAEQLYQ"
BASE COUNT          553 a         1135 c         1142 g          499 t
ORIGIN      
        1 ggggctccac gacgcgggac tcaccgattc cgcccgtgtc gtggtcgaga agccattcgg
       61 ccacgatcgc gaatcggcga tcgcgctcgc cgaggagatc caccagtaca tcgacgagtc
      121 gcagctgtac cggatcgacc attacctcgg gaagatgggc ctggtcgaga tcctgcacct
      181 acgattcgcg aacgcgatgc tggagccggt ctggagccgc aacttcttgt cgagcgtcca
      241 gatcacgatg gccgagtcgt tcggcgtcga ggaccgcggc cacttctacg atcccgtcgg
      301 cgcgctgcgt gacgtcgtcg tgaaccacct catgcaggtg gtcgcggcgt cggcgatgga
      361 ggcgccatcg cgcggagacc cgtcgacgat caaggacgcc caggtcaatc ttttccgcgc
      421 cgtcgcagag gccgaccccg ctcactacgt gcgcgggcag tacgagggat atcaggcgat
      481 cgacggcgtg gcgcaggact cgacgaccga gacgttcgcg gctctgcgtc tggacatcga
      541 gagctggcgc tgggcgggag ttcccttctt catccgaacc ggcaagcgcc ttccgatcac
      601 ccagaccgag ctgcggcttg tgttcaagca ccctccgaag ctcgggttcg gggtcctgga
      661 tcgccggccc gacgagaatc agctcgttgt ccggctggac ccgacgaccg gcatccggct
      721 gaccgtcgag gcacaccggg ccgacctgga gggcgccgcc cccgtccacc tggacatgga
      781 gttcgctacg gagggcgggg agggtccgac gccctacgag gtgttgctcg atgcggcgat
      841 gcgtggcgac agcacgcggt tcacccggca ggacggggtc gaggagacct ggcgcgtgtt
      901 ccagcctttg ctcgattcgc cgccgcccgt ccacccctac gctccgggaa cgtgggggcc
      961 ggatgccgct aacgggctcc tggaggggtt cggaacctgg cacgagccct gggtgagccc
     1021 gtgagccggg ccgccgaacc gacgaccggc ggcgcgcaga gcgcggcgat gccgtcgccg
     1081 ttcccgccga tcgccgacta cgccttcctc tccgactgcc acaccggagc cctcatcgcg
     1141 ccggacggcg cggtcgactg gttgtgcgtc ccgaggtacg actcgccgag catcttcggg
     1201 agcctgttgg accgacaggc gggggcgttc cggttcggcc cgtacggcat caacgtcccc
     1261 tcggagatcg cctatgaacc cgggacgaac gtcctgcgga cgacctggaa gacgccgtcc
     1321 ggttgggtgg tcgttcgcga cgcgctcacg atgggagcgc gcagcggcga ggacacggtg
     1381 acgccacaca cccgaccgcc ggccgacgac gacgccgacc ataccctcgt gcgcacggtc
     1441 gagtgcatcg acgggcaggt cgaggtcgag ctcgtctgcg agccgatctt cgattacggc
     1501 cgggtcgtcg cgagctggac ggttgaggac ggagagggac acaccgcgga cgcgatgggc
     1561 ggcgacctga cgatcaggct tcagacggac ctagccatgg gcgcggaagg cgaccgcgtc
     1621 cgcgcccgcc acgtcctgaa cgccggcgac cgcgccttct gctcgctgtc ctggggccac
     1681 gacctcaccg gcccggccga cgtcgacgag gccgaggcgc ggatcgacgc caccgtccgg
     1741 ttctggcgac ggtggctcgg cagggcgcgc atccccgacc accgctggcg cgaaccgatc
     1801 cagcgttcgg cactggtggt caaggggctc acctacatgc cgacgggagc aaccgtggcg
     1861 gcccttacga cctcgctccc cgagaccccc ggaggcgaac ggaactggga ctaccggttc
     1921 acctggatgc gtgattcgac cttcacgctg caggccctcc actggctcaa cctggactgg
     1981 gaagcggacg agttcatgca gttcgtcgcc gacgtcgagc ccaacgacga cggcgggatg
     2041 cagatcatgt acgggatcga cgggcgccgg gacctcacag agtcgacgct cgacgagctt
     2101 tcggggtacg cgggcgcgcg tccggttcgg atcggcaacg gcgccttcga ccagcgccag
     2161 aacgacgtct acggggccgt cctcgactcg atcctcatgc acacgcgccg gagccaacgg
     2221 cttccgcgcc gcctgtggcc catcgtccag agccaggccg agtgcgcgac gcgcgtgtgg
     2281 cgggagccgg accagggcat ctgggaggct cgaggcgcgc cacagcagta cgtgtcctcg
     2341 aagctgatgt gctgggtcgc actcgatcgg gcggcgaagc tggcccagat cgggggggac
     2401 tccgacctct cgcagacctg gggcaagact gccgaagaga tccatcagga catcctcgat
     2461 cacgggatga gcgaccgcgg cgtgcttcga cagcattacg acacggacgc gctcgacgcc
     2521 tcgacgctgc tcgcgcccct cttcgggttc cttcccggta ccgacgaacg gatgcacgcc
     2581 tccgtgctcg cgatcgccga cgagctcacc gagaacgggt acgtcctgcg gtacaagacg
     2641 gaccagaccg acgacgggat gtcgggaaag gaggggacct tcctgatctg ctcgttctgg
     2701 ttggtctcgg ggcttgccat cgtcggcgag gagcaacggg cccgcgacct gatggaacgc
     2761 ctcttgcgca tctcttcccc gctcgggctg tacgcggagg agtacgacgt cgagacgggg
     2821 cagcatctcg gcaacacccc gcaggcgttc tcgcacctcg ccctgatcga ggcggcgggg
     2881 cggatcatcc tgggcgagcg cgtcgccgag ctgtgacgga cccccacgag ggaggcaagc
     2941 agatgagcga ctacgacgtg atcatcatcg gcaccggcgc gggcggtggg accctcgccc
     3001 gacacctggc cccgtcgggc aagcgcatcc tcctgctcga gcgcggaggc tggttggcgc
     3061 gcgagccgca gaactggagc acggcggacg tcttcatcga cggccggtac atctcgcccg
     3121 acacgtggta cgacgccaag ggcaggacct tccagccgca ggtccactac ttcgtaggcg
     3181 gggccaccaa gctgtacggc gctgcgctct accgcctgcg ggagaaggac ttcggagagc
     3241 tcaagtatcc cgatgggatc tcccccgcgt ggccgatcgg ctactccgac atggagcagt
     3301 actacacgct ggccgagcag ctctaccag
//
LOCUS       DASUYD010000019         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_231787, whole genome shotgun sequence.
ACCESSION   DASUYD010000019 DASUYD010000000
VERSION     DASUYD010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231787"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF195_01080"
     CDS             complement(<1..299)
                     /locus_tag="VF195_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX6579460.1"
                     /translation="MDTTVGLRERKKLRTRAELSDAAFRLFAERGFDETTIEDIVEQV
                     EVSPRTFFRYFDSKEDVVIGFFDDMGLELRAMLAERPPDEPPFTALRGALGSLIDL"
     gene            436..693
                     /locus_tag="VF195_01085"
     CDS             436..693
                     /locus_tag="VF195_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579461.1"
                     /translation="MTAYVLVQTATQDGGIARRLREVPGIVFAEDVRGPYDALALARS
                     DVMGDAFKTILDQIRGLPGVIHALAAPVAREPAGMSSVNAA"
     gene            1000..1122
                     /locus_tag="VF195_01090"
     CDS             1000..1122
                     /locus_tag="VF195_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579462.1"
                     /translation="MHEHPNIAIVRRTLEVDNSGDQRAAEILANDQDPVDAFWS"
     gene            1125..1346
                     /locus_tag="VF195_01095"
     CDS             1125..1346
                     /locus_tag="VF195_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579463.1"
                     /translation="MTVEHPNATRIRQTVGAFMAGDIPLPLDGFAENVLWYSPGDTPA
                     SGRLRGREGVLRFFDDGRGVRRDPADRSG"
     gene            1597..2067
                     /locus_tag="VF195_01100"
     CDS             1597..2067
                     /locus_tag="VF195_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579464.1"
                     /translation="MDAATFWKFLHIACMFLAVSIFVGQGMLSGTVARSGDVRAIRRV
                     LAAEDRFAPVGGGVFLLGIVFGFVTAIVGDFDLTQTWLVIGYVLAALILVTAFAYHIP
                     TATKLKVLADASPDDEPSAELRAFVDAPIGRVVNVIDGVLWLALIYVMVAKPFS"
     gene            2148..>2552
                     /locus_tag="VF195_01105"
     CDS             2148..>2552
                     /locus_tag="VF195_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579465.1"
                     /translation="MRVLVVEDEVKMAGLLKRALEEEGYAVDVAGRGQDAVWLGTENP
                     YDAIVLDLMLPDLDGFEVSRQLREVGRWSPVLMLTARDAVADRVAGLDAGADDYLTKP
                     FSFAELLARLRALMRRGAAERPPVLRAGDLELD"
BASE COUNT          410 a          840 c          878 g          424 t
ORIGIN      
        1 aggtcgatca acgatccgag tgcgccccgc agcgccgtga acggcggctc gtccgggggt
       61 cgttccgcca gcatcgcgcg gagctccagc cccatgtcgt cgaagaagcc gatcaccacg
      121 tcttccttgg agtcgaaata ccggaagaac gtccggggtg atacctcgac ctgctcgacg
      181 atgtcctcga tcgtcgtttc gtcgaagccg cgctccgcga agagtcggaa cgcggcgtcg
      241 gagagctccg cccgcgtccg cagcttcttg cgttccctga ggcctaccgt cgtatccacg
      301 ggcacagcat agcacttggc gaccactgca atctggcagg ggttgccaaa tggcacggaa
      361 ccacgtaggg tggcggccac gaagggccag ggccagaaga cgggcaggac ggcagaggga
      421 aggagttcga gggtcatgac ggcatacgtg ctcgtgcaga ccgctacaca ggacggcggg
      481 atcgcccgcc ggcttcggga agtcccgggg atcgtgttcg ccgaggacgt ccgcgggccc
      541 tacgacgccc tcgcgctcgc gcgttcggac gtcatggggg acgcgttcaa gacgatcctc
      601 gatcagatcc gggggctgcc aggtgtgatc cacgcgctcg cggctccggt cgcgcgtgag
      661 ccggccggca tgtcgagcgt caacgcggcc tgagatcctc ccgcccgaga tcgcccttcg
      721 gtgcccgcac cgaggacgtc aaggccacgt actcggacgg cattctcgag gtggtcgtgc
      781 ccggtgcggc gaagcggtcg acgcaacgcg tccccgtcga ggtcggatca gagaaggaga
      841 agaagtaggg ccgaggtcgt aggaccgaag taggaccgag gcccgcggca gcgtcggttc
      901 tcagacccag gacatgcgcc gtttcccgta gcccccgcga ccctggcagc gctcacgtcg
      961 ccgggtacgg tgctggcact gccccctgtg gaggaaacgg tgcacgagca tccaaacatc
     1021 gcgatagtcc gtcgcacact cgaggtcgac aactccggcg atcaacgcgc tgcggagatc
     1081 ctcgccaacg atcaggaccc ggtcgacgcg ttctggtcct gaggatgacg gtggagcacc
     1141 cgaacgcgac tcggatacgg cagacggtcg gcgcgttcat ggccggcgac atcccgctcc
     1201 cgctcgatgg attcgcggag aacgttctct ggtactcccc cggggacacg ccggcgagcg
     1261 gcagactccg ggggcgcgag ggcgtcctgc ggttcttcga tgatggacga ggcgtccggc
     1321 gggaccctgc ggatcgaagc ggatgacgtc ctcgccggag accgctacgt gacgaccttc
     1381 ctcaccgtca cggcgctccg tcacatcgag cggatgagcg tggtcgtcgc gcagctggcg
     1441 gaggtcgacg aacacggcag gtgtcccgat gctggttcct gccggacaag ctcgatgagt
     1501 ggaacggttt cttcgccgga gcgaactccg acagacatta gcctcggtgc tcgccggagt
     1561 tgcgacgcgc tcatcaggac gatctagcat ggctgggtgg atgcggcgac gttctggaag
     1621 ttcctgcaca tcgcgtgcat gttcctggcg gtgtcgatct tcgtcgggca ggggatgctg
     1681 tccgggaccg tagcgcgctc cggcgacgtc cgggcgatcc gccgcgtgct ggcggccgag
     1741 gaccgcttcg cccccgtcgg tggtggcgtc ttcctgctcg gcatcgtgtt cgggttcgtc
     1801 acggccatcg tcggagactt cgatctgaca cagacgtggc tggtgatcgg ctacgtcctc
     1861 gccgcactca tcctggtaac cgcgttcgcg tatcacatac cgaccgcgac caagctgaaa
     1921 gtgctcgcgg acgcgagccc cgacgatgag ccctcggccg agctgagagc gttcgtcgac
     1981 gccccgatcg gccgcgtggt gaacgtcatc gacggtgtcc tgtggctcgc gctcatctac
     2041 gtgatggtcg cgaaaccgtt cagctaggcc accgaaccag gtccgccgtt tctcggatgt
     2101 ccgcgtctct catccggctc tcatcggcga agcctacgct gtgggcgatg cgcgtgctcg
     2161 tggtggagga cgaggtcaag atggccggcc tgctcaagcg cgcgctcgaa gaggagggct
     2221 acgcggtcga cgtcgccggc cgtggccagg acgccgtgtg gctcgggacc gagaacccct
     2281 acgacgccat cgtcctggat ctgatgctgc ccgacctcga cggcttcgag gtgagccggc
     2341 agctgcgcga agtggggcga tggagccccg tcctgatgct taccgcgcgg gacgcggtcg
     2401 ccgatcgcgt agcgggtctg gacgccggcg ccgacgacta cctgaccaag ccgttctcgt
     2461 tcgccgagct gctggctcgc ctccgcgcgc tgatgcgacg cggggccgcc gagcgccccc
     2521 ccgtccttcg cgccggcgat ctcgagctcg ac
//
LOCUS       DASUYD010000020         3838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_453066, whole genome shotgun sequence.
ACCESSION   DASUYD010000020 DASUYD010000000
VERSION     DASUYD010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453066"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VF195_01110"
     CDS             <1..286
                     /locus_tag="VF195_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579466.1"
                     /translation="VFFVTANSTLQLTSRPDMRGRVMALYGIVFLGTTPFGAPVAGWI
                     GEHLGPRIAFAGGGLVALATGLVGLWVLSRRATAGRLELDPTGIGRVEAA"
     gene            complement(300..1043)
                     /locus_tag="VF195_01115"
     CDS             complement(300..1043)
                     /locus_tag="VF195_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6579467.1"
                     /translation="MPRDWDAETYDRISDPQFRWGASVVGWLDLDGAETVVDAGCGSG
                     RVTELLAERLLRGRVVALDGSASMIEQAERRLRRFGDRIRFVVADLLHPLPLAEAADA
                     VLSTATFHWIPDHDALFANLAAALRPEGRLAAQCGGAGNLESVVRALEEVGADPFTDK
                     VFATPERTSERLRVAGFADAECWLHPEPTPFETLEGLETFLRTVALGAHVATMGDDEA
                     GAFVHAVAQRLPRLELDYVRLNIRARRAG"
     gene            complement(1053..2660)
                     /locus_tag="VF195_01120"
     CDS             complement(1053..2660)
                     /locus_tag="VF195_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579468.1"
                     /translation="MKKTGLVLLTLASGQFLMTLDSSVMNVSIATVAKDVGTTVTGIQ
                     TAITLYTLVMAMLMIPGGKVGSLIGRKRAFSIGCVIYGAGSLTTALAQNLTVLIFGWS
                     LLEGIGAALILPAIVALVAGNFPPAGRPRAYGLVMAAGAIAVAVGPLIGGIATTYFSW
                     RWVFVGEVLIVLVILVLARRVEDAPVEGRPDLDLVGAMLSATGLGLGVFGVLRSSVWG
                     WVLPKPGGAEIFGLSPTIVLIFGGLFVLWLFAAWERHVEQRGGEPLVQLSMFENRQFT
                     GGLLMFFFQFLVQAGLFFTIPLYLSVALGLSAIDTGIRILPLSITLLIAAAGIPRFFP
                     NVSPRLVVQAGLLAMFAGTLSLFLSIDVDAGAEIVTVPLLLAGLGIGALASQLGSVTV
                     SAVPDEQSPEVGGLQNTATNLGASIGTALAGSLLIAALTASFLSGIEQNPDVPSEVTA
                     QANVELAAGIPFISDADLETALEKAGASADVTRAVLDENEQARLDGLRTALAALALIA
                     LIGLFFASRIPTEQPGATQQRAAPQQA"
     gene            3074..3370
                     /locus_tag="VF195_01125"
     CDS             3074..3370
                     /locus_tag="VF195_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579469.1"
                     /translation="MSERGVLLHRDDPITRTLRSTRQTELTWADIRSVDAETRVRAST
                     RSRVWGIVISFVLIDGGRKRQVVGYASVSTTRLLTEMRRFAEMGEHRIEWPDIA"
     gene            complement(3374..3685)
                     /locus_tag="VF195_01130"
     CDS             complement(3374..3685)
                     /locus_tag="VF195_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579470.1"
                     /translation="MGDASNANDGKGDDSFAGWWLGGFGCAGAIVAGVLAAVLANLAF
                     VDMAGMVALGGVLGVVIGLRVAATLVLLADELGWDETPHASHIGRLYLRLDRLIDRAG
                     L"
BASE COUNT          649 a         1290 c         1333 g          566 t
ORIGIN      
        1 cgtgttcttc gtgaccgcga actcgacact gcagctgacc tcgcggcccg acatgcgcgg
       61 ccgtgtgatg gcgctgtacg ggatcgtgtt cctcgggacg acgccgttcg gagccccggt
      121 tgccgggtgg atcggcgagc acctcggccc gcggatcgcc ttcgccggcg gcgggctggt
      181 cgcgctcgcg accgggctgg tcggcctgtg ggtcctctcg cggcgcgcga ccgccgggcg
      241 gctcgagctc gacccgaccg ggatcgggcg cgtcgaagcg gcctgagttt cggcttccgc
      301 tagcccgcgc gccgggcgcg gatgttgagc cgcacgtagt ccagctcgag tcgtggcagc
      361 cgttgagcga cggcgtgcac gaaggccccg gcctcgtcat cgcccatcgt cgcgacgtgg
      421 gcgccgaggg cgaccgtccg caggaacgtc tcgagcccct cgagggtctc gaacggcgtg
      481 ggctcgggat gcagccagca ctccgcgtcc gcgaacccag ccacccgcag acgctccgag
      541 gtgcgctcgg gcgtcgcgaa caccttgtcc gtgaacgggt cagcaccgac ctcctccagc
      601 gcccgcacca cggactcgag gttccccgca ccgccgcact gggctgcgag ccgtccttct
      661 gggcggaggg ccgctgcgag attcgcgaac agcgcgtcgt ggtcggggat ccagtggaag
      721 gtcgcggtcg aaaggaccgc gtccgcggct tcggcgagcg ggagcgggtg cagcaggtcg
      781 gcgaccacga accggatccg gtcgccgaaa cgccgcagcc tccgctccgc ctgctcgatc
      841 atcgacgccg agccgtcgag cgcgacgacc cggccgcgga ggagccgctc cgccaggagc
      901 tcggtgacgc gtcccgagcc acacccggcg tccacgacgg tctcggctcc gtcgaggtcg
      961 agccacccga cgacgctcgc accccaccgg aactgcggat cggagatccg gtcgtaggtt
     1021 tcggcgtccc agtcgcgcgg caccgtgacc gatcacgcct gctgcggagc agctcgctgc
     1081 tgcgtggctc ctggctgctc cgtcgggatc cgggatgcga agaacaggcc gatcaacgcg
     1141 atcagggcga gggccgcgag ggcggtccgg agcccgtcga ggcgcgcctg ctcgttctcg
     1201 tccaggacgg ccctcgtgac atcggcgctg gcacccgcct tttcgagcgc cgtctcgaga
     1261 tcggcgtcgg agatgaacgg gatcccggcg gcaagctcga cgttggcctg ggcggtcacc
     1321 tcggatggga cgtcggggtt ctgctcgatc ccgctcagga acgacgcggt gagcgccgcg
     1381 atcaggaggg agccggcgag ggccgtcccg atcgaggctc ccaggttggt cgcggtgttc
     1441 tggagcccgc cgacctcggg gctctgctcg tcggggaccg ccgagaccgt cacgctgccc
     1501 agctgcgagg ccagcgcacc gatccccagg ccggcgagca ggagcggcac cgtcacgatc
     1561 tcggcgccgg catccacgtc gatactcagg aacagcgaca gggtcccggc gaacatcgcc
     1621 aggagccccg cttggacgac gagtcggggc gagacgttcg ggaagaaccg tgggatgcct
     1681 gcggcggcga tcaggagggt gatggacagg ggcaggatcc tgatgcccgt gtcgatcgcg
     1741 gagagcccga gggccacgga gaggtacagc gggatcgtga agaagagccc ggcctggacc
     1801 aggaactgga agaagaacat caggaggccg cccgtgaact gccggttctc gaacatcgac
     1861 agttgcacga gcggctcgcc gccccgctgc tcgacgtgac gctcccacgc ggcgaacagc
     1921 cacagcacga acaggccgcc gaagatcaag acgatggtcg gtgacaggcc gaagatctcg
     1981 gccccgccgg gcttgggcag cacccatccc cagacgctcg accggagcac gccgaagacc
     2041 ccgagcccga gcccggtggc cgagagcatc gcgccgacga ggtcgagatc cggccgtccc
     2101 tccaccgggg cgtcctcgac tcggcgggcc agcacgagga tcaccagcac gatcaggacc
     2161 tccccgacga agacccatcg ccacgagaag taggtggtcg cgatccctcc gatcagcgga
     2221 cccaccgcca ccgcgatcgc cccggccgcc atcaccaggc cgtatgcgcg aggccggccc
     2281 gccggcggga agttgccggc gacgagcgcg acgatcgccg ggaggatcag cgcggcgccg
     2341 atcccctcga gcaacgacca cccgaagatg aggacggtga ggttctgcgc gagggccgtc
     2401 gtcagggagc ccgcgccgta gatcacgcac ccgatcgaga aggcccgctt gcgcccgatc
     2461 agcgagccca ccttcccacc cgggatcatg agcatcgcca tcacgagcgt gtagagggtg
     2521 atcgcggtct ggatcccggt cacggtcgta ccgacgtctt tggcgacggt cgcgatcgac
     2581 acgttcatga cggagctgtc gagcgtcatg aggaactgac cggacgccag cgtcaggagc
     2641 accagtccgg tcttcttcac ccgagcgttc gtcacatgcg gatggtatcc gggagccggc
     2701 cgttctcatc gggatgctgc ggggacgaga gatcatgaag aatcgctggt cgctgctcct
     2761 aacgcggttg tggcgttgaa gcccaatccg tgaagagttg tgcgggtcgc gacgatggta
     2821 atgccatgtg gagctcgatg ccgacggccc gtggcccggt tcggcgagac gaagtgttcg
     2881 tggtgtatcc gcaccgcggc ttcacggtcg cggtggggac ggcgagcctg ctgataggtg
     2941 tcgcctgcct gatcgcggcg atcgtgatct tgtcatccga ccccagcggc tctgcaggcg
     3001 caactggcat cggctcgtct tctgcttctc ggcctggtac gtcttcgtgg caaggcgatg
     3061 tcctcagata accgtgtcgg agcggggtgt gctgctgcac cgcgacgatc ccatcacacg
     3121 gacgctgagg agtacgcggc agaccgagct tacgtgggcg gatatccgta gcgtcgatgc
     3181 cgagacgcgg gtgcgggcgt cgacccgctc ccgggtttgg gggatcgtga tctccttcgt
     3241 gctcatcgac ggcggccgca agaggcaggt ggtcggctac gcaagtgttt ccacaacacg
     3301 gctgcttacg gagatgaggc gattcgccga gatgggcgag catcggatcg agtggcccga
     3361 catcgcgtga ccttcacagg ccagcgcggt cgattaacct atcgaggcgt agatagagtc
     3421 gaccgatgtg cgaggcgtgg ggcgtttcgt cccacccaag ttcgtcggcc agcagcacca
     3481 acgtcgcggc cacacgcagc ccgatcacca ctcccaagac cccgcccagg gcaaccatcc
     3541 ccgccatgtc gacgaaagcc aggttcgcta acacggcggc cagcactccg gcaacgattg
     3601 caccagcgca cccgaacccg ccgagccacc agccggcgaa ggaatcatct cctttgccat
     3661 cgttggcgtt cgaagcgtcg cccataacac ctattcggca aagacacgag gcaacctgaa
     3721 ggcagaacga ctgcgaggag gactggacgc tccgagcggt gcttgtcggg atgttgcgag
     3781 aggttgaccg atgagcccag gacgattcca aggtgcgtag tcgccttgtg ggatcgac
//
LOCUS       DASUYD010000021         5173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1334125, whole genome shotgun sequence.
ACCESSION   DASUYD010000021 DASUYD010000000
VERSION     DASUYD010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5173
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334125"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="VF195_01135"
     CDS             complement(<1..885)
                     /locus_tag="VF195_01135"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6579471.1"
                     /translation="MLGPALPTAQLKHERLGKPTALAVFASDNLSSSAYATEEILKVA
                     VPAVGAVAFSLVMPITFAILAVLAILLFSYRQTIKAYPSAGGAYIVTKDNFGLLTAQL
                     AGVALLTDYVLTVSVSVAAGVAAITSAIPGTFPYRVWMSVFFIWFIAWGNLRGVRESG
                     RMFMVPTYFFVVMMFVLLGTGLARAFSGDLQQLPLPDDVAATTGAIGVFLVLHAFASG
                     GAAVTGVEAISNGVPAFKPPEWVHARSTLMWMGSLLGLMFLGLSFLALKLHALPTETE
                     TLNSQIAKAVFGSGGFGTA"
     gene            1101..2576
                     /locus_tag="VF195_01140"
     CDS             1101..2576
                     /locus_tag="VF195_01140"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579472.1"
                     /translation="MSDRWVLAPGGGSRVRAVVTSLVAPSLALGLALIVQPERELGAV
                     SVFLMAVVAASVAGGIWAGIGSSVLGFLALNYFFTEPLHTFRVHNRDDVVALVVFFVV
                     ALLVGWVVARAVSERDRATRREREARLLSLFATKALSGEPLDRVLNDLAAALVDALRL
                     ASCAIRAHAAGRTYEVRRTRPGTTEGVAVEVPISSGDVGFGTLTATRPAREEFPSEDR
                     RLLESAASQIAVGLERASYDAEIAAARLDAERSQARAALFSSVTHDLRTPLASIKAAV
                     TSLLQVEVRFDPAQARELLQTVLEETDRLNRLVGNILGLAQVRSGALQPVKEPTALDE
                     IVEAVLHRLEPRFGAIRVRTIFRDAPSVPVDPVLIDQVLSNLMENAIRFSPPAGEVTV
                     SVVPWRSAVQVRVADQGPGIAREERDRVFEPFTQLGTTTSDATGGSGLGLAISRAIVL
                     AHGGRIWIEGTPGGGAAVVFELPARETAPVPQESAAREGGR"
     gene            2573..3250
                     /locus_tag="VF195_01145"
     CDS             2573..3250
                     /locus_tag="VF195_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6579473.1"
                     /translation="MTRVLVVDDEPQIRRAMRTSLAAHGYDVRTVGTGEEAVIAAAED
                     APELVLLDLGLPDLDGTEVIRRVRSFSAVPIIVLSVRDRQADKVAALDAGADDYVTKP
                     FGVDEVLARLRAALRRSQAGEPIPPVLRFGEVEVDVGRRLVTVADTPVHLTKTEYSLL
                     EAFVTNPGKLLTHQWLLRKVWGPGYAQESHYLRVYVRSLRRKLGDEASAPRLILTEPG
                     VGYRWIG"
     gene            3320..>5173
                     /locus_tag="VF195_01150"
     CDS             3320..>5173
                     /locus_tag="VF195_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6579474.1"
                     /translation="MATQTRPGSSVLKRVFVGRAKASSHLEHTLLPKILALPVFSSDP
                     MSSVAYATEEIMIVLLAVSSAGKEYVFPIAIAIATLLGIVVISYRQTVRAYPNGGGAY
                     IVSKENIGTGAGLVAAAALLVDYVMTVVVSVVAGVYAITSAAPSLVGSRVWMSVAFVI
                     FITLANLRGVRESGTLFAIPTYGFIAIVLVMIGLGIVRCLGGCPAGDAIPPAPEVANT
                     VQAISLFVILHAFSSGSTALTGVEAISNGVPAFRRPQSKNAAETLAVMGVIAITMFLG
                     ISWLATHIQNVTVSEERTVIAQIGHSVFGGGVGFYLLSFFTASILILAANTAYQDFPR
                     LSAILARDRYMPRQFMNRGDRLVFSNGVIGLAFFASVIIVAFNANLTQLIQLYVVGVF
                     TSFTLSQTGMVRHWLREKHKGSEAQPGWRRSIVINTIGAITTGIVLIVITSTKFVHGA
                     WLSILAMAVLVPTFYMIHRHYGEVMAQLRQGKVRAGTVGVNHMVLVIRDLDAAAAEAV
                     GVVRSIRPTELRVVHPCDGEIPPELQDRWRTFSMGAPRLEPLVLGKDSLLDALRRYVR
                     RVERGPSDFVNVVIPEVVSGSMLGYLLRGAGLVRLKAGLLREENIVVTDVPV"
BASE COUNT          804 a         1686 c         1793 g          890 t
ORIGIN      
        1 ggccgtgccg aaacccccgg acccgaagac cgcctttgcg atctgcgagt tcagcgtttc
       61 ggtctcggta ggcaacgcgt ggagcttcag tgcgaggaac gacagaccga ggaacatcag
      121 tcccagcagg cttcccatcc acatcagggt gctccgggca tggacccact ccggcggttt
      181 gaaggccggg accccgttcg agatcgcctc gacgccggtc accgcagcac cgccggaagc
      241 gaaggcgtgg aggacgagga agacgccgat ggctcccgtg gtcgcggcga catcgtcggg
      301 cagcggcagc tgctgtaggt cgccgctgaa tgcgcgtgcc agacccgtcc cgaggaggac
      361 gaacatcatc acgacgaaga agtacgtcgg aaccatgaac atccgtccgg attcgcggac
      421 cccgcgcagg ttgccccaag cgatgaacca gatgaagaag acggacatcc agacgcggta
      481 cgggaacgtc ccggggatcg ccgacgtgat cgcagcgacc ccggcggcaa ccgagaccga
      541 caccgtgagc acgtagtccg tgaggagagc cacgcctgcg agctgtgcgg tcagcagccc
      601 gaagttgtcc ttcgtgacga tgtaagcgcc gccggcgctc ggataggcct tgatggtctg
      661 ccggtatgag aacaggagga tcgcgaggac cgccaggatc gcgaacgtga tcggcatgac
      721 gagcgagaag gcgacggctc cgacggccgg aacggccacc ttcaggatct cctcggttgc
      781 gtacgcggag gatgaaaggt tgtccgaggc gaagacggct agggctgtcg gcttgccgag
      841 ccgttcgtgc ttgagctgtg cagtcggcag cgccggtccg agcacgagac gcttccaaag
      901 atgcgcgggc cccctgggtt gagggctgcg tctggatgtg ggcgcctcgg tcgccgcgtg
      961 cggctgcggt tcgagctgtc gaagtttggt ggccacggtg tcccctcgtc accccaagcc
     1021 tcggccgggt cgcgtaaacg acctgtgaaa ggcgaagggg ctcgcgtgaa gaacccgtag
     1081 cgaagccgta gcgtgacctc atgagcgacc ggtgggtgct ggctccgggt ggtgggtcgc
     1141 gggtccgggc cgtcgtgacc tcgctcgtgg caccctcgct cgcgctcggg ctggccctga
     1201 ttgtgcagcc tgaacgcgag ctcggggcgg tctccgtgtt cctaatggcc gtagtggccg
     1261 cgtcggtcgc gggggggatt tgggcgggga tcggcagctc ggtgctcggt ttcctggccc
     1321 tcaactactt cttcaccgaa cccctccaca ccttccgcgt ccacaaccgg gacgacgtgg
     1381 tcgcgctggt cgtcttcttc gtggtggccc tgctcgtggg gtgggtggtc gctcgcgctg
     1441 tttccgaacg cgaccgcgcg acccgtcgcg agcgcgaggc ccgcctgctg agtctgttcg
     1501 cgaccaaggc gctgtcgggc gagccgctgg atcgggtgct gaacgatctg gcggcagccc
     1561 tcgtggatgc gctccgcctg gcgagttgcg cgatccgagc ccacgcggcg ggtcgcacct
     1621 acgaagtgcg caggacgcgt cccggcacga ccgagggggt ggccgtcgag gtcccgatct
     1681 cgagcggcga tgtcgggttc ggaaccctca ccgccacacg ccctgcccgc gaggagttcc
     1741 cttccgagga ccgccggttg ctcgagtcgg cggcaagcca gattgccgtc ggcctcgagc
     1801 gcgccagcta cgacgcggag atcgcggccg cccggctcga tgcggagagg agccaggcga
     1861 gggccgcgct gttctcctcg gtgacgcacg acctccgcac gcccctcgcg tccatcaagg
     1921 ccgcggtgac cagcctgctg caagtcgagg tgcggttcga cccggcgcag gctcgggagc
     1981 tcctacagac ggtcctcgag gagaccgatc ggctgaaccg gctcgtgggc aacatcctgg
     2041 ggctcgcgca ggtgcgctcc ggtgcgctgc aaccggtgaa ggaacccacc gcgctcgacg
     2101 agatcgtcga ggctgtcctt caccgactcg agcccaggtt cggggccatc cgggttcgga
     2161 cgatcttccg cgacgccccc tccgtcccgg tcgacccggt cctgatcgat caggtcctct
     2221 cgaacctgat ggagaacgcg atccgcttct cgccaccggc cggcgaggtc accgtctcgg
     2281 ttgtgccgtg gcgttctgcc gtgcaggtac gggtggcgga tcagggcccg ggaatcgcgc
     2341 gggaggagcg cgatcgcgta ttcgagccgt tcacgcaact cgggacgacc acttcggacg
     2401 cgacgggtgg aagcggtctc ggactggcga tctcccgagc gatcgtgctc gcgcacggcg
     2461 gaaggatctg gatcgaaggg acgccaggcg gtggggctgc cgttgttttc gagctcccgg
     2521 cccgcgaaac ggccccggtc ccgcaggaat cggcggctcg ggagggaggc cgttgacgcg
     2581 cgtgctcgtc gtcgacgacg agccccagat ccggcgcgcg atgcggacca gcctggcggc
     2641 acacggctac gacgttcgca ccgtcggcac gggagaggag gctgtcatcg cggcggcgga
     2701 ggacgctccc gagctcgtcc tcctcgacct cggcctcccg gatctcgacg gcaccgaggt
     2761 gatccgacgc gtgcgatcct tttcggcggt tccgatcatc gtgctctccg ttcgggatcg
     2821 acaggccgac aaggtagcgg cgctcgatgc gggagcggac gactacgtca ccaagccgtt
     2881 cggggtagac gaggtgctcg cgcgtctccg cgccgcgctc cgcaggagcc aggcggggga
     2941 gcccattccg ccggtccttc gtttcggtga ggtggaggtc gatgtcgggc gccgactggt
     3001 gacggtggcc gacacacccg tccacttgac gaagaccgag tactcgctgc tcgaggcgtt
     3061 cgtgacgaac ccagggaaac tcctcacaca ccagtggctc ctgaggaagg tctgggggcc
     3121 cggctacgcg caggagagcc actacctacg tgtctacgtg cgctcattac gacgcaagct
     3181 tggggacgag gcctcggcac ctcgtctgat cctgaccgag ccgggcgtgg ggtatcgatg
     3241 gatcggctga gcatcgccga gagcaacagc cacagcgcgc acccgtccat gcggttcggg
     3301 tcgtagcatc tgctcccgta tggccaccca gacccgtccc gggagctcag tcctgaagag
     3361 ggtcttcgtc gggcgggcga aggccagcag ccacctcgag cacacgctgc ttccgaagat
     3421 ccttgcgctc ccggtcttca gctcggaccc gatgtcctcc gtggcgtacg cgaccgagga
     3481 gatcatgatc gtgctcctgg cggtgagctc cgcgggcaag gagtacgtgt tccccatcgc
     3541 gatcgcgatc gcgacgctgc tcgggatcgt ggtgatctcc tatcgacaga ccgtccgtgc
     3601 gtacccgaac gggggcggcg cgtacatcgt cagcaaggag aacatcggga cgggcgccgg
     3661 gctcgtcgcg gcggcggcgc tgctcgtcga ctacgtgatg acggtggtgg tctcggtcgt
     3721 cgcgggcgtc tacgcgatca cgtccgccgc gccgagcctc gtcggatctc gcgtgtggat
     3781 gtccgtggcg ttcgtcatct tcatcacgct ggcgaacctt cgcggtgtgc gcgagtctgg
     3841 aacgctcttc gcgatcccga cgtacgggtt catcgcgatt gtcctcgtga tgatcggctt
     3901 ggggatcgtc cgctgcctgg gcgggtgccc ggcgggcgat gccatcccgc ccgcgcccga
     3961 agtggcgaac accgtgcagg cgatctcgct gttcgtgatc ctgcatgcgt tctcgtcggg
     4021 ctcgacggca ctcaccggcg tcgaggcgat ctcgaacggc gtgccggcgt tccgacgccc
     4081 gcagtccaag aacgccgccg agacgctcgc cgtgatgggc gtgatcgcca tcacgatgtt
     4141 cctcgggatc tcctggctcg cgacccatat ccagaacgtc acggtgagcg aggagcggac
     4201 cgtgatcgcc cagatcgggc actcggtgtt cggaggcgga gtcggcttct atctcctgtc
     4261 gttcttcacg gcctcgatcc tgatcctcgc cgcgaacacc gcgtaccagg acttcccccg
     4321 cttgtcggcg atcttggcac gtgaccgcta catgccccgc cagttcatga accgcggaga
     4381 tcggctcgtc ttctcgaacg gcgtgatcgg cctggcgttc ttcgcgagcg tgatcatcgt
     4441 tgcgttcaac gccaacctga cgcagctcat ccagctctac gtggtcgggg tgttcacctc
     4501 gttcacgctg tcgcaaacgg gcatggtccg gcactggctc cgtgagaaac acaagggctc
     4561 ggaggcgcag ccggggtggc gacggtcgat cgtcatcaac acgatcggag cgatcacgac
     4621 gggcatcgtg ctcatcgtca tcacgtccac gaagttcgtc cacggggcat ggctgtcgat
     4681 cctggcgatg gccgtactcg taccgacgtt ctacatgatc caccggcatt acggggaggt
     4741 gatggcgcag ctcaggcaag gcaaggtccg cgcggggacc gtcggcgtca accacatggt
     4801 gctggtgatc cgagatctcg acgccgcggc cgcggaggcg gtcggggtcg tccgctcgat
     4861 ccgtcccacc gagctgcggg tcgtgcatcc gtgcgacggc gagatcccac ccgagctcca
     4921 ggatcgctgg cgcacgttct cgatgggtgc tcctcgcctc gagccgttgg ttcttgggaa
     4981 ggacagcctc ctcgatgcgc tccgacggta cgtgcgacgc gtcgagcgcg gtccgagcga
     5041 tttcgtgaac gtcgtcatcc ccgaggtcgt gagcggctcc atgctcggct acctcctgcg
     5101 cggagcgggg ctcgtacgac tgaaggcggg gttgctccgt gaggagaaca tcgtcgtgac
     5161 ggatgtcccc gtg
//
LOCUS       DASUYD010000022         9737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_453515, whole genome shotgun sequence.
ACCESSION   DASUYD010000022 DASUYD010000000
VERSION     DASUYD010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453515"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF195_01155"
     CDS             complement(<1..183)
                     /locus_tag="VF195_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579475.1"
                     /translation="MRRYDASEIEPKWVERWEAEGLYRASEDPADPRPRFYALDMFSY
                     PSGDLHMGHAEAYSGGD"
     gene            complement(245..1021)
                     /locus_tag="VF195_01160"
     CDS             complement(245..1021)
                     /locus_tag="VF195_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579476.1"
                     /translation="MADELEGRIDELYGLPLERFTAARDALAKELATDGDREGAARVK
                     GLRKPVVAAWAVNVLAREDPDGVKELIELGGTLRSAHRRAVSGGDVEPFRVATEERRQ
                     LVRTLTRAAQAIVERDGGAPGAATDAIAGTLEAATVDEETGELLRSGRLTKPVRPPAT
                     FSGAGLRVLEGGPSRRPEPQEAPKPKPNRTKLRRDLSAAQTRERKAEVAVERERATIQ
                     DLDRKRSETKDRLRAAEAELRGVSLERKRLASALDKLGDA"
     gene            complement(1034..1567)
                     /locus_tag="VF195_01165"
     CDS             complement(1034..1567)
                     /locus_tag="VF195_01165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6579477.1"
                     /translation="MVREPILIAPASADDLEAISDIYDHYVRTTAISFDLEPPTTAWR
                     REWFERFGDLGRHRLLVARDGGEAIGYAASVPYRPRAAYGPSVETSVYVSPTHLGRGV
                     GSALYGALLSELEREDVHRAYAGIAMPNPASVRLHERWGFRRAGYFTEQGRKFGRYWD
                     VAWFERGFPEVARATSG"
     gene            1881..2762
                     /locus_tag="VF195_01170"
     CDS             1881..2762
                     /locus_tag="VF195_01170"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6579478.1"
                     /translation="MKTGPEAGRRVELDLEVAIGRQDGDLVVEDPEVSRRHAVLRRSG
                     GSVVVEDLDSTNGTFVNGERIRTPITVGPGDQVRVGRTMLEIEPDQRADDTIVSTPPR
                     PDQIRTAEAPPSGGVLVEEHEDATQPLPSRALERGSRPARSNGRWVGLGAAALVVLLG
                     VIAYVALVDRPAENDFASRANEACAAVQRAEGGMDLGRTPTRGELQGARNVRLQALGA
                     IRVLERPEQDDAVSVSRFLSAFAETNASINALERAIGGDNRAVVRAVGKLRGDVRDER
                     ELAAKAGIAGCGGLAIR"
     gene            2814..4196
                     /locus_tag="VF195_01175"
     CDS             2814..4196
                     /locus_tag="VF195_01175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6579479.1"
                     /translation="MGDVLGRSLMSEVRRGEDTLLGRPVAVKLLLDYGDPRSVALFQQ
                     EAQILARVQHPNVISVFDAGVDGGDRFIVMELVEGPTLRELLETEGRLGPERAGEIAS
                     RLASALAFAHRKAVIHRDVKPSNVLLPPDGEVKLADMGIARLLSPEALTATLSVRGTA
                     LYISPEQVRGDRVDHRADLYSLGCVLFEMLTGRTPFEGDLAALSYAHTHTPAPRARSI
                     NPAVPAAMDDLVAAMLEKDPARRPPTGEEVERSLTAAFRPDSAVPIASTERMQPTPSP
                     TPRPPAGGRRRSGPKTRPMVVIAVCGLAVLLFIALLASREPDGRADATQPSPQPSSST
                     ATQQPSPSETSSPSTQQPSTVETSSPAPEEPSPQEAAAVVLDTVAEGIETGEITDHFD
                     HDIRHTLDEILRDSDKGEDVDKLLEKVDDLREKVSEALEKGEVTSADRASAIDEALLK
                     FAEALSDAEQ"
     gene            4499..4684
                     /locus_tag="VF195_01180"
     CDS             4499..4684
                     /locus_tag="VF195_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579480.1"
                     /translation="MKHTEVSGLAALIVWFGTPMRVRGAVFRGAWMATLVVAALVTVS
                     FGIAGFVVLVDWALLHG"
     gene            complement(5007..6035)
                     /locus_tag="VF195_01185"
     CDS             complement(5007..6035)
                     /locus_tag="VF195_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579481.1"
                     /translation="MSAVSGKNVWAVGNYGGKTSILHWNGLRWSRVPSPNPGSGIRDL
                     NAVSAVSPTDVWAVSDTSYLSETLILHWDGTRWSRVTSPNPGPDSNGLIGVSATSATD
                     AWAVGSAGTFLGLYLRYRTLVLHWDGTTWSQAASPNPSEGGNVLFGVSAESATDAWAV
                     GYDDIEGETETLILHWDGSGWSRVIGPNPGSGSNKLSGVSAVSPTGAWAVGDSRGFAT
                     SVHETLILHWNGSRWSQVKSPSPGASFNALTGVSAVSPTDAWAVGIYRDEDEIGHTLA
                     LHWDGTRWSQVKTPNPGSVFNELSSVSAVSPKDAWAVGYYQEAGVHALVLHWNGTKWS
                     QADVHRVR"
     gene            complement(6399..7157)
                     /locus_tag="VF195_01190"
     CDS             complement(6399..7157)
                     /locus_tag="VF195_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579482.1"
                     /translation="MPSLLDTATCTAVRASDSYHRASRRSVVSRIATFVIGFVLGGFV
                     VGGAIYLSDTQDDSTSEPIEQPSVSGSLPAQDSPTPAPIGTDDPSVSPTATVAVATPV
                     TEADELRIDGLGSMTIGMHVNDVERATGMEVEISSDFSPECRYGQLVGGPNDLFLMFS
                     RRVLVRIDIVTGSAIETDTRIGVGDPISAVEAAYGDELERELHPYLGDGGSYLIFDPE
                     PEDGLLIIFETNRRSVTSFRSGFDEQVQYKEGCA"
     gene            complement(7396..7611)
                     /locus_tag="VF195_01195"
     CDS             complement(7396..7611)
                     /locus_tag="VF195_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579483.1"
                     /translation="MHGSLVVALVAGGWIVSVLGPRGAYAIGGVTGLVGAALLLPFLK
                     WLPEHKADVGAVQELPVVERVPFDPPA"
     gene            complement(7611..8636)
                     /locus_tag="VF195_01200"
     CDS             complement(7611..8636)
                     /locus_tag="VF195_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579484.1"
                     /translation="MTPPRTAVRRLALGRAISVAGTFAAGTALLYTIYRETQSTAWIS
                     ATMLLTWAVIGFFSPLAGALGDRFDRRRVMIVSESAAALCWFAMAFVTQSPAVLLAIA
                     FASSVVEVAYFPASSAAIPNVAGEEHLSWANSLLSVGRYAGLTFGPIAGGLLVAVIGP
                     EWVFAANGFSYLASVALTWSVRADFADPERDKASEEHRGVLAGFRFVRRDRVLRLLAL
                     SFMVFIVGMATTLVADPVLADEFGTGSLGFGLITALWGAGTIAGVWLGRRITEESEGR
                     WLVGCSVLVALTGFGVALSPWFGLVLFWMFAFGATDGPTQVIEQNLLQRRSPDVVRSR
                     VMVRGRR"
     gene            complement(9316..9391)
                     /locus_tag="VF195_01205"
     tRNA            complement(9316..9391)
                     /locus_tag="VF195_01205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9356..9358),aa:Ala,seq:ggc)
     gene            complement(9401..>9737)
                     /gene="rsfS"
                     /locus_tag="VF195_01210"
     CDS             complement(9401..>9737)
                     /gene="rsfS"
                     /locus_tag="VF195_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX6579485.1"
                     /translation="AAAKQAVDVTILDVHGLIVITDYFVICSGQTDRQIKTLVEEVER
                     AVREIGEKPIRREGDADSRWVLLDYIDVVVHVFAREEREYYDLERLWRDAPRLGWNAT
                     DQAAAGSLD"
BASE COUNT         1667 a         3315 c         3211 g         1544 t
ORIGIN      
        1 gtcgccgccg ctgtaggcct cggcgtggcc catgtggagg tcgcccgagg ggtaggagaa
       61 catgtcgagc gcgtagaacc gcggccgtgg gtccgccggg tcctccgagg ctcggtagag
      121 accctcggcc tcccagcgtt ctacccactt cggttcgatc tcggacgcgt cgtagcggcg
      181 catggtcagc agatgctacc gaacggaccc cgcccgatcg gcgaggcgag cgctcgatcg
      241 tccgtcaggc gtccccgagc ttgtccaacg cggacgcgag acgcttgcgc tcgagcgaga
      301 cgccgcggag ctcggcctcg gcggctcgga gccggtcctt ggtctccgag cgcttccgat
      361 cgaggtcctg gatcgtcgcg cgttcgcgct cgacggcgac ctcggccttg cgctcgcgtg
      421 tctgggcggc cgagaggtcg cgccgcaact tggtgcggtt cggcttcggc ttgggagcct
      481 cctgcggctc cggccggcgg gacggtcctc cttcgagcac ccggagtccg gcgccggaga
      541 aggtcgcggg cggacggacc ggcttcgtga gcctgcccga ccggaggagc tcacccgtct
      601 cctcgtcgac cgtcgccgcc tccagggtcc cggcgatcgc atccgtcgcc gccccgggcg
      661 caccaccgtc gcgctcgacg atcgcttggg ccgcgcgtgt gagcgttcga accagctggc
      721 ggcgttcctc ggtcgccacc cggaacggtt cgacgtcacc gcccgacacc gctcgtcgat
      781 gcgccgaacg cagcgtgccg cccagctcga tcagctcctt gacgccgtcc gggtcctccc
      841 gagcgaggac gttcaccgcc caggccgcga cgacgggctt gcggagcccc ttgacgcggg
      901 cggcgccctc gcgatcgccg tcggtcgcga gctctttcgc aagcgcgtct cgtgcggccg
      961 tgaagcgctc gagcggaagc ccgtagagct cgtcgatccg tccttcgagt tcgtcggcca
     1021 cacccccaac ctctcacccg gaggtcgcgc gcgccacctc cgggaagccc cgttcgaacc
     1081 acgccacgtc ccagtatcgc ccgaacttgc gcccctgctc ggtgaagtag ccggcccggc
     1141 ggaaccccca ccgctcgtgg aggcgcaccg acgccgggtt gggcatcgcg atccccgcgt
     1201 acgcgcggtg gacgtcctcg cgttcgagct cggacagcag cgcgccgtag agcgcggacc
     1261 cgaccccgcg ccccagatgt gtgggcgaga cgtacacgct ggtctcgacg gaggggccat
     1321 acgcggctct cggccggtat ggcacgctcg ccgcgtagcc gatcgcctcc ccgccgtccc
     1381 gagcgacgag gaggcggtgg cgtccgaggt cgccgaacct ctcgaaccat tcccgccgcc
     1441 acgcggtcgt cggcggttcg aggtcgaacg agatcgcggt cgtcctaacg tagtggtcgt
     1501 agatgtccga gatggcttcg aggtcgtcgg ccgaggccgg ggcgatcagg atcggttcgc
     1561 gtaccacccg cccatccttt caggcgtgcg cggatggatg ggttaccacg tggttacgag
     1621 tgtgccgatc actacctcgg cctgccgatt acgaggcgac cttgctgaag tagccgtccg
     1681 ggtcctgaag tagctgcagt cttgagccgc cgcctcgcgt tcgtacaggc cagcgacgac
     1741 gatccctcac cgagagagca cctgccctgt accatccgac gtcaaggtcc gctgacacgc
     1801 gaccgataac gaacgagccg cacgatcgtc gtagtggtgt cgttcgccaa tcctgggggt
     1861 gagggttgcc aacggttgtg gtgaaaaccg gtccggaggc gggcaggcgg gtcgaactcg
     1921 acctcgaggt ggccatcggt cgtcaggacg gcgacctcgt ggtggaggat cccgaggtgt
     1981 cgcgccgtca tgcggtgcta cgacgctcgg gcggatcggt ggtcgtcgaa gacctcgact
     2041 cgaccaacgg caccttcgtc aacggggaga ggatccgaac ccccatcacg gtgggtcccg
     2101 gtgaccaggt cagggtcgga cgtaccatgc tcgagatcga gccggaccag cgagccgacg
     2161 acacgatcgt gtcgacacct ccgcggccgg atcagatccg aaccgccgag gcgccaccgt
     2221 ccggtggtgt cctcgtggag gagcacgaag acgcgaccca gcccttgccc tcgcgggcgc
     2281 tcgaacgcgg ttcgcgaccc gcgcgctcga acgggaggtg ggtcgggctc ggggccgcag
     2341 ccctggtcgt gctcctgggg gtgatcgcct acgtggcact cgtcgaccgg ccggccgaga
     2401 acgatttcgc ctcccgagcc aacgaagcgt gcgcggccgt acagcgggcg gaaggaggca
     2461 tggatctcgg ccggacccca acgcgcggag aactccaggg cgcccggaac gtgcgactcc
     2521 aggcgctcgg ggcgatccgt gtgctcgagc ggcctgaaca agacgatgcc gtgtcggtga
     2581 gcaggttcct gtccgccttc gcggagacga acgcatccat caacgccttg gaacgagcga
     2641 tcggtgggga caaccgcgcg gtcgtccgcg ccgtcggaaa gctccgaggg gacgtcagag
     2701 acgaacggga gcttgcggcg aaggccggta tcgcgggatg tggaggtctt gcgatccgtt
     2761 aggaaggacg acccgacatc cagacgcctc gactgatcgg cgaccgctac cgcctggggg
     2821 acgtgctcgg acggagcctg atgtccgagg tccgccgggg cgaggacacc ctgttggggc
     2881 ggccggtagc cgtcaagctg ctcctcgact acggggatcc ccgctcggtc gccctgttcc
     2941 agcaggaggc ccagatcctc gcccgcgtcc aacacccgaa cgtgatcagc gtgttcgacg
     3001 ccggggtcga cggcggtgac cgtttcatcg tgatggagct cgtcgaggga ccgacgctcc
     3061 gggagctgct cgagacggag ggccggctgg ggcccgaacg agcaggcgag atcgcgagcc
     3121 gcctggcctc cgccctagcg ttcgctcacc gaaaggccgt catccaccgg gacgtcaaac
     3181 cgtcgaacgt gctgctcccg cccgatggag aggtgaagct cgcggacatg gggatcgccc
     3241 gactcctgtc cccggaggcc ctcaccgcca ccctgagcgt gcggggcacc gctctgtaca
     3301 tctcgccaga gcaggtgcga ggtgaccggg tcgaccatcg agcggacctg tactccctgg
     3361 gctgtgtgct gttcgagatg ctgaccggcc ggacgccgtt cgagggagac cttgcggcac
     3421 tttcgtatgc acatacccac acccctgcgc ctcgtgctcg ctcgatcaac cccgccgttc
     3481 cggccgcgat ggacgacctt gttgccgcca tgctcgagaa ggatcccgcc cggcgcccgc
     3541 caaccggaga ggaggtcgag aggtctctca ccgccgcctt caggcccgac tctgccgtgc
     3601 cgatcgcgtc gacggagcga atgcaaccga caccctcacc gacgcctcgc ccgcccgcgg
     3661 gtgggcgtcg gagatccgga ccgaagacac ggccgatggt cgtcatcgcg gtgtgcggcc
     3721 tcgccgtgct cttgttcatc gcgctgctgg caagccgcga accggacggc cgagccgatg
     3781 cgactcagcc atcgccgcag ccgagcagct caaccgcgac gcagcagcct tcgccgtcgg
     3841 agaccagctc gccctcgacc cagcagcctt cgacggtgga gaccagctca ccggctccgg
     3901 aagaaccgtc gccccaggaa gccgctgcgg tcgtgctcga taccgttgct gagggcatcg
     3961 agaccggcga gatcaccgac catttcgacc atgacatccg acacacgctc gacgagatcc
     4021 tccgcgacag cgacaagggt gaagacgtgg acaagttgct cgagaaggtc gacgatctgc
     4081 gagagaaggt gtccgaagcc ctcgagaagg gcgaggtcac ctccgcagac cgcgcatcag
     4141 cgatcgatga ggcgctcctg aagttcgcgg aagcccttag cgacgccgaa cagtgaccgc
     4201 atgcggcgaa cccaacagat cgtgggctga ggcgacggct cgcaggtgca agtaggcggc
     4261 tcgggtatcc gtggatcaag ccaccgaaca catcgcgcct gcggacgctt cagccggcgc
     4321 gggcgtagca accccgatcg gtccgtggcg accccgatcg agcatcaaat gccgcgtggg
     4381 cctttcaagg cggctgcatc ctgccgaagt aatccctatg ggtcgaagac cgcgagctcc
     4441 gtacgacgag cttcccgtcc cgacggagcc agagagtcgg ctctccaccg agccgtggat
     4501 gaagcacacg gaggtgtcgg gcctcgctgc cttgatcgtc tggttcggca cgccgatgcg
     4561 cgtacgaggg gccgtgtttc gcggcgcatg gatggccacg ctggtcgtcg ccgccttggt
     4621 gacggtttct ttcggaatcg ctggcttcgt tgttctagtc gactgggcac tgctgcacgg
     4681 ctgatctcgc cgggcgtcag gagctaagga gagccgttcc acttatggta gctattgact
     4741 gagattgcct tgtgagagtc gcgagcatgc gcgaatgccg ccagagccgc tgtaagctgc
     4801 gaccccgctt ctcgtctcag agggatgtct atcgggtccc cggatttcag gatggggacg
     4861 gagcggtggc acaagaagat gctagtgcgt tcatgcactg gtgccgggcg gtgttggtcg
     4921 ggatgctgag ggaaatggag cggaggagct cacgcccgcc aacatcgctg tttgtggagt
     4981 agacgctgat cgttgcacac agcgactcac cgaacgcgat gaacgtcggc ctgcgaccac
     5041 ttcgtgccat tccaatgaag aaccaaggcg tggacaccag cctcctggta gtaacccacg
     5101 gcccaggcgt ccttgggcga gacggcgctc acgctggaga gctcgttgaa cacggagcca
     5161 gggttcgggg tcttgacctg cgaccacctt gtgccatccc agtgaagggc cagcgtgtgg
     5221 ccgatctcat cctcatctcg gtagataccc accgcccatg cgtcggtggg cgagacggcg
     5281 ctcacgccgg tgagtgcgtt gaacgaggcg cccgggctcg ggctcttgac ctgcgaccag
     5341 cggctgccgt tccagtgaag gatcaacgtt tcgtggacac tggtcgcgaa gccgcgggag
     5401 tcaccgaccg cccatgcgcc ggtgggcgag accgcgctca cgccggagag tttgttcgac
     5461 cccgagcccg ggttcgggcc gatgaccctc gaccagccgg atccgtccca gtgaaggatc
     5521 aacgtttcgg tctcaccctc gatgtcgtcg taaccgacgg cccaggcatc cgtggccgat
     5581 tcggcgctga caccgaagag gacgttgccg ccttcgctgg gattcgggct cgctgcctgc
     5641 gaccacgtgg ttccatccca gtgcaggacc agcgtgcggt agcggaggta caggccgagg
     5701 aaggtgcccg cactcccgac cgcccaggca tcggtggccg aggtagcgct caccccgatg
     5761 agcccgttgg agtccgggcc cgggttcgga ctcgtgaccc gcgaccagcg cgtgccgtcc
     5821 cagtgaagga tcaacgtctc gctgagatag gaggtgtcgc tgaccgccca gacgtcggtg
     5881 ggcgatacgg cactcacggc gttgaggtcc ctgatgcctg agcccgggtt cgggctgggg
     5941 acccgcgacc agcgcagacc gttccagtgg agaatcgatg tcttgccccc gtagttcccg
     6001 accgcccaga cgttcttgcc ggagaccgcg ctcacgccgc ggagaggagt tgcggtcggt
     6061 cccggatgag ggttgtggcg cgatgacgta caggacccgc tcgtgatggc accggcgacc
     6121 aagacgcagg cggcggcgat ccaccccact cgccatcgcc cggttcgtgt cctcctgcga
     6181 agacccgccg aaaaggaacg ccaacggcta ggcctttccg cgatggcggc gtggtcggtc
     6241 atcggatgag cgtcttgtaa gcggttcagg tcgggccgtc aaccggcaga gagctccaac
     6301 ggggactcgg gactcttctt gagctcccga tcgactggga ggaggacccg acgcttcgag
     6361 cggtgctggc cgacgagttg cgggagccag ctggaagcct aggcgcagcc ttccttgtac
     6421 tgaacctgct catcgaatcc cgaacgaaac gacgtcacgc tgcgtcggtt ggtctcaaag
     6481 atgatcaaca ggccgtcctc gggttcggga tcgaagatga ggtagctgcc gccatctccc
     6541 agatagggat ggagctcgcg ttcgagctca tcaccgtacg cggcttcgac tgccgagatc
     6601 ggatccccca cccctatcct cgtatctgtt tcgatcgccg acccggtaac tatgtcgatc
     6661 cgcaccagca cacgccgact gaacatcagg aagaggtcat tgggaccgcc gaccaactgc
     6721 ccgtatcgac actcagggga gaagtccgac gagatctcta cctccatccc ggtcgcacgc
     6781 tcgacatcgt tgacgtgcat tccgatcgtc atcgacccaa gcccgtcgat acgaagctcg
     6841 tccgcctcgg tcaccggagt agcgacagcc acggtcgcag tcggggaaac ggatggatcg
     6901 tcggtaccga ttggagcggg agtgggtgag tcttgggcag gtagagatcc agagacactt
     6961 ggctgctcga tcggctcaga cgtcgaatcg tcctgagtgt ccgacaagta gatcgccccg
     7021 ccgacgacga agccgccgag gacgaacccg atcacgaagg tggcgatcct gctcactacg
     7081 ctcctcctgc tggcgcgatg atacgaatcc gacgcgcgga cggcggtaca ggttgccgtg
     7141 tcgagcaggg agggcatcga aagacgaggt cgggggctgg cgaccagccg acgttcagat
     7201 cgtgctcagc actgttggga atcccaagat ccgcgtcttc gccgagcccc tgatcgactg
     7261 cgaggaggac cgttcgtttc cgggcggtgc tgggcgggat gctgcgggag gccgagacgc
     7321 ggtgagggcg atcgccctcg agtatcgccg cgcacgccga taggtcccag ccaaaggaac
     7381 cgggcgaact gcccgctacg cgggtgggtc gaacggcacc cgctccacca ccgggagctc
     7441 ctgtacggct ccgacatcgg ccttgtgttc cgggagccac ttcaaaaacg gcagcaggag
     7501 cgcggcgccc acgagtccgg tcaccccgcc gatcgcgtac gcgcccctcg gtccaagcac
     7561 ggagacgatc cagccgcctg cgacgagcgc cacgaccagg gacccatgca tcagcgtctc
     7621 ccacgcacca tcacccggct ccggaccacg tccgggctcc ggcgctggag caggttctgc
     7681 tcgatcacct gcgtcggccc gtcggttgcg ccgaacgcga acatccagaa cagcacgagc
     7741 ccgaaccacg gtgacagcgc gacgccgaac ccggtaagcg cgaccagcac gctgcaaccg
     7801 accagccacc gtccctccga ctcctcggtg atccgtcggc cgagccacac ccccgcgatc
     7861 gtcccggctc cccacagcgc ggtgatcagg ccgaacccga gcgaccccgt tccaaactcg
     7921 tcggcgagca ccgggtcggc aacgagcgtc gttgccatgc cgacgatgaa caccatgaac
     7981 gaaagcgcga gcaaccgaag gacgcggtcc ctgcgcacga accggaatcc agcgagcacc
     8041 ccgcggtgct cttccgacgc cttatcccgc tcggggtcgg caaagtccgc ccgaacgctc
     8101 caggtgagcg cgaccgaagc aaggtatgag aagccgttcg cggcgaacac ccattcgggg
     8161 ccgatcacgg ccaccagcag gcctcccgcg atcggcccga aggtgagacc cgcgtagcga
     8221 ccgaccgaga gcaggctgtt cgcccaggag agatgctcct ccccggcgac gttggggatc
     8281 gcggccgagg aggccgggaa gtacgccacc tcgaccaccg acgaggcgaa ggcgatggcg
     8341 aggagcaccg cgggagactg ggtcacgaac gccatcgcaa accaacacaa cgccgcggcg
     8401 gactcgctca cgatcatcac ccgccgccgg tcgaaccggt cgcccaatgc gcccgctagc
     8461 gggctgaaga agccgatcac cgcccacgtc agcagcatcg tggccgagat ccatgcggtg
     8521 gactgcgtct cccggtagat cgtgtacaac agtgccgtcc cggctgcgaa ggtcccggcc
     8581 accgagattg cccggccgag cgccagccga cggacggcgg tccggggcgg cgtcaccccg
     8641 ctcgctcctc gagtacttcg ttcgatggag tgagttccaa gcgatcttcc tcagtcgata
     8701 agactccgtt gagctcccgt gcggcctcat ctaccgtgac tgctcgccgg cacacctgca
     8761 agtcatctcg accaacgtga tgcgggctac cggtgacggt tggccacggt gttcccaacg
     8821 gctcaagggg tgtggaaccc ctaccaggac ttctccccgg gccgaagcga tccgctccta
     8881 ctggtgaacg ccgcgacggg gtcctccagc gaactgatcc tcttcgagga agaactgttg
     8941 ctccgcgtca agctccgttc ctcatcgccc cacgtcccgc accgagccat ctcgctcacg
     9001 cacccttgat ccgggcgacc aggacctgct caccggccag gatcgccacg cggttgctct
     9061 ggcccctcag caggtcgacc gacgtgatgg cgccgctgac ggcgacggtc accgggacct
     9121 ggatgatcct gaactggtcg cccgccgacg acctgcccga cctcttggct cgcttcgatc
     9181 cgaatcagcg caccagggga tgggcgactg atctcctgga actcagccaa acgtatggcg
     9241 gggtcacgac accctgacgg tcgcgcgttc gcattggctt ggatcggctg ggagtgagcc
     9301 catcttttgt gcaggtggtg gacccaaggg gattcgaacc cctgaccttc tggctgccag
     9361 ccagacgctc taccagctga gctatgggcc cgcgggagcg tcagtctagc gaaccggccg
     9421 cggcctggtc cgtagcgttc caacccaggc gcggcgcatc gcgccacagg cgctcgaggt
     9481 cgtagtactc gcgctcttcc cgggcgaaca catggacgac gacgtcgatg tagtcgagca
     9541 gcacccaccg ggagtcggcg tcgccttcgc gccggatcgg cttctcgccg atctcgcgga
     9601 cggccctctc gacctcctcg accaacgtct tgatctgccg gtcggtctgg cccgagcaga
     9661 tcacgaagta atcggtgatc acgatcaggc cgtgcacgtc caagatcgtg acgtccaccg
     9721 cctgcttggc ggccgcg
//
LOCUS       DASUYD010000023         5661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1554893, whole genome shotgun sequence.
ACCESSION   DASUYD010000023 DASUYD010000000
VERSION     DASUYD010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5661
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554893"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VF195_01215"
     CDS             complement(<1..877)
                     /locus_tag="VF195_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX6579486.1"
                     /translation="MARPGFVLEVDERTPPLLVHEGEGFRLQKFPLGTRVVYPPDALP
                     GIRDVNAAIRHALLHPLGDSEPLPELLKAGMKLTIAVDDISIPLPPMTLPDIRQRILE
                     QVIELAARKGVEDVQIVVATALHRRMTADEIRRMVGERVFRSFWPKDLFNFDGEDKDA
                     LTEIGTTDEHEVVEISKRAAESDLLVYVNVNLVAMDGGHKSVAIGLAGYRSLKHHHNV
                     DTMMHSRSYMDTRPGHSAIADSAARMGGLIAAEGPKVFQIETTVNSETFPKNLGFLNK
                     REWEWSAYDQGLMIAA"
     gene            complement(895..2103)
                     /locus_tag="VF195_01220"
     CDS             complement(895..2103)
                     /locus_tag="VF195_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006539939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6579487.1"
                     /translation="MALALEFYRSAPKYLGARTIGSKAPGLLTGGLAPLRLVTQKDPE
                     PTREGWARVKPRLSGICGSDLSTIAGRSSFYFSPLVSLPFVPGHEVVGELLDECGDLK
                     AGQLVVISSVLGCAARGEDPPCEHCAAGHVGRCDRVTVGHLKPGLQTGYCSETGGGWS
                     RLMLAHRSQLWLVPDGMGDETAVLIEPLACAIHAAFRARIPAGGTVHVVGAGTVGILT
                     LIAIKAFTKADHVVVSAKHQRQRAAASMAGADDVVRPEHAVKAVRRTDHAVKLTPERG
                     MEFLLGGVDVAIECTGSSSALDLALRTTKAGGRIVVSGIPGGGADLTPLWFRELELAG
                     AYTSGVEDLGDGVTRPTFEMAIELATNLPMLEQLVGATYPLDRWRDALDHAMAAGSLG
                     TFKVAFAPQD"
     gene            complement(2109..4436)
                     /locus_tag="VF195_01225"
     CDS             complement(2109..4436)
                     /locus_tag="VF195_01225"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX6579488.1"
                     /translation="MVDKGAQEAKRKAKRSPRASSIVERLAGKRVFMTGVTGFLGQVV
                     LERLLADFPETTVVLLVRSQTGASSVERVHYLTRKPSFDRLRESLGSDDALLHLLDER
                     IEVIDGDFTRGRPDLPGDIDVVIHSAATVSFDPPIDEGFQTNLLGAMNLYGAVLDAGS
                     RPALVHVSTAYVAGVQKGVIPERTLDHRVDWRLEADLAMSARRDVEAASRKPELLARF
                     LEDASKEHSRAGPTTVADDAEQRRKDWMTKRLVEYGRQRARSLGWPDVYTFTKAMGER
                     AVEELAAAEQLPLSIVRPSIIESALLHPAPGWIDGFKMADPIIRAYGLGQIPEFPGIP
                     EGIVDIIPVDFVVNAILAVAATPPTPGEPAHYHVSSGDRNPLQFHQLYALVREYFEAH
                     PLPERGRGEHKVPEWTFPGNVTVEKMLRRAERLTDVAEQIVTHLPKSKGMRDAVRRVD
                     RDKARVDFVKRYSDLYGAYTEAEVIYTDDVAMQLFRSLSTEDRARFPFDAATIDWKHY
                     LQDVHSPAVTQSLRELARRDRARPSVKIREREEPVLAVFDMEGTIISSNVVESYVWAR
                     MADLEPDGWPGELVSVFGRIPRYLAIDRRDRGDFLRTFFRRYEGASVEGLERLVDRHV
                     GEFMLQKASPGAIRRIREHRAAGHRTLLLTAAANAFLRPLRPLFDVVIGADLQVDAEG
                     RYTGFMSTPPLVGEARAAWLRRYAATEGVDLKGSYAYADSYSDLPLLRAVGNPVAVSP
                     DSHLYRYARRRRWPIEEWSMSKGMPRVRFPKPARR"
     gene            4574..>5661
                     /locus_tag="VF195_01230"
     CDS             4574..>5661
                     /locus_tag="VF195_01230"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX6579489.1"
                     /translation="MSTIPLIVLVFAVTYAVSAQRTASRTNEDVDRTTGVRRELAEIQ
                     HDLAIAESSVRGSVLTGQARMREEYGEAVIELRRDLEDLKARVTEPLQRNRLEPLEAL
                     VEERLATLQGVLRIGRDRTPEAQDRLETFLLHGQTITNAIRGLTEQMELTASELMAER
                     IAARDAAFARSYLIQVIAMPAAIFVAMILMASFTGGIVKRVAKMGENAYRLDKGQPLD
                     RPDSSNDELGSLSRAIVRTGAHLSDLQEDLRRHATVDDLTGLANRRGFFALGEHELLV
                     AARTRSSVALLFIDVDGLKRVNDELGHVAGDQLLKESADVIRETVRASDIAGRLGGDE
                     FCVLLIGDPELDPDRVVERMRDTLASHNA"
BASE COUNT          889 a         1984 c         1920 g          868 t
ORIGIN      
        1 gggccgcgat catcaatccc tggtcgtacg cggaccattc ccactcgcgc ttgttcagga
       61 atccgaggtt cttcgggaac gtctccgagt tgacggtggt ctcgatctgg aagaccttcg
      121 gaccctccgc ggcgatcagc ccgcccatcc gcgcggcgga atcggcgatc gcgctgtggc
      181 cggggcgggt gtccatgtag gagcgcgagt gcatcatcgt gtcgacgttg tggtggtgct
      241 tgagggagcg gtagcccgcg agtccgatcg cgaccgactt gtgaccgccg tccatcgcca
      301 cgaggttcac gttgacgtag acgaggaggt cggactcggc ggcccgcttc gagatctcga
      361 ccacctcgtg ctcgtccgtg gtgccgatct ccgtcagtgc gtccttgtcc tcaccgtcga
      421 agttgaagag gtccttcggc cagaacgacc ggaacacgcg ctcccccacc atccgccgga
      481 tctcgtcggc ggtcatccga cgatggagag cggtcgccac gacgatctgg acgtcctcga
      541 cgcccttgcg cgccgcgagc tcgatcacct gctcgaggat ccgctgccgg atgtcgggca
      601 gggtcatcgg cgggagcggg atcgagatgt cgtccaccgc gatcgtgagc ttcatccccg
      661 ccttcagcag ctcggggagc ggctccgaat ccccgagcgg gtgcagcagc gcgtggcgga
      721 tcgcggcgtt cacgtccctg atgccaggca gggcgtcggg cggatacacc acacgggtgc
      781 cgagggggaa cttctgcagg cggaagccct ccccctcgtg gacgagcaga ggcggtgtcc
      841 gctcgtccac ctccaacacg aatccgggtc tggccatctg tcgtcgtctc ctcttcagtc
      901 ttgcggcgcg aacgccacct tgaacgtgcc gagcgagccc gcggccatcg cgtggtcgag
      961 ggcgtcgcgc caccggtcga gcggatacgt cgcgccgacg agttgctcga gcatcgggag
     1021 gttcgtggcg agctcgatcg ccatctcgaa cgtcggtcgg gtgaccccgt cgccgaggtc
     1081 ctcgacgccg gacgtgtagg ccccggcgag ctccagctcg cggaaccaca ggggcgtgag
     1141 atcggcgccg ccacctggga tgccggagac gacgatgcga cctcccgcct tcgtggtgcg
     1201 gagcgcgaga tcgagcgcgc tgctcgagcc cgtgcactcg atcgccacgt cgacaccgcc
     1261 gagaaggaac tccatgccgc gctccggcgt gagcttcacc gcgtgatccg tccggcgcac
     1321 ggccttcacg gcatgctccg gccggacgac gtcgtcggcg ccggccatcg aggccgctgc
     1381 gcgctggcgt tggtgcttcg ccgacacgac cacgtgatcc gccttcgtga acgccttgat
     1441 cgcgatcagc gtcaggatgc cgactgtgcc ggccccgacg acgtgtaccg tcccgcccgc
     1501 agggatcctc gcgcggaacg cggcgtggat cgcgcacgcg agcggctcga tcagcaccgc
     1561 ggtctcgtcc cccatcccgt cgggcaccag ccagagctgg gatcggtggg cgagcatcag
     1621 acggctccac cccccaccgg tctccgagca gtacccggtc tggagccccg gcttcaggtg
     1681 gccgaccgtg acgcggtcgc accggcccac atggcctgcc gcgcaatgct cacacggcgg
     1741 atcctctcca cgagcggcgc acccgaggac gctcgagatc acgacgagct ggcccgcctt
     1801 caggtcgccg cactcatcca ggagctcacc gacgacctcg tgtccgggca cgaacgggag
     1861 cgacacgagc ggtgagaagt agaacgagga gcgcccggcg atcgtggaga ggtcgctgcc
     1921 gcagatgccg gagaggcgtg gcttcacgcg cgcccacccc tcgcgcgtgg gctccggatc
     1981 cttctgggtc acgagccgga gcggcgcgag cccgccggtg agcaaaccgg gcgccttcga
     2041 cccgatcgtg cgcgcgccga ggtacttcgg cgcggagcga tagaactcga gcgccagcgc
     2101 caccgtccct acctcctcgc cggcttcggg aatcggacgc gcggcatccc cttcgacatc
     2161 gaccactcct cgatcggcca ccggcgccgg cgcgcgtacc ggtacaggtg cgaatcgggc
     2221 gacacggcga cggggttccc gaccgcgcgg agcaggggca ggtcggagta ggagtcggcg
     2281 tacgcgtagg aacccttcag gtcgacgccc tcggttgcgg cgtagcggcg gagccaggcc
     2341 gcccgcgcct cgccgaccag cggcggggtg gacatgaacc cggtgtagcg cccctcggcg
     2401 tccacctgga ggtcggcccc gatcacgaca tcgaacagcg gccgcagggg acggaggaac
     2461 gcgttcgctg cggcggtgag caggagcgtt cggtgcccgg cggcccggtg ctcccggatc
     2521 cggcggatcg ccccggggct ggccttctgg agcatgaact cgccgacgtg ccggtcgacg
     2581 agccgctcga gtccctcgac cgacgcgccc tcgtaccggc ggaagaacgt ccgcaggaag
     2641 tccccgcgat cacggcggtc gatcgccagg tagcggggga tcctgccgaa gacgctcacc
     2701 agctcgcccg gccatccgtc cggttcgagg tcggccatcc tcgcccacac gtaggactcc
     2761 acgacgttgg aggagatgat cgtgccctcc atgtcgaaga cggcgagcac cggctcctcc
     2821 cgctctcgga tcttgaccga gggccgagcg cggtccctcc gagcgagttc gcggagcgac
     2881 tgggtcacgg ccggcgaatg gacgtcttgg aggtagtgct tccagtcgat cgtcgcggcg
     2941 tcgaacggga agcgggcccg atcctccgtc gacaggctcc ggaagagctg catcgccacg
     3001 tcgtcggtgt agatcacctc ggcctccgtg tatgcgccgt agaggtcgga gtaccgcttc
     3061 acgaagtcga cgcgggcctt gtcccgatcc acgcgccgaa cggcatcccg catccccttc
     3121 gacttgggga gatgggtgac gatctgctcc gcgacgtcgg tcagtcgctc cgcccggcgc
     3181 agcatcttct ccacggtcac gttccccggg aacgtccact ccgggacctt gtgctcacct
     3241 cgaccccgct cggggagcgg atgcgcctcg aagtactcac ggacgagcgc gtagagctga
     3301 tggaactgga gcgggttccg atcgcccgag gacacgtggt agtgggccgg ctccccgggc
     3361 gtcggcggtg tcgcggccac cgcgaggatc gcgttcacca cgaagtcgac cgggatgatg
     3421 tcgacgatcc cctccgggat gcccgggaac tcggggatct gccccagccc gtacgcccgg
     3481 atgatcggat cggccatctt gaagccgtcg atccaccccg gcgccgggtg caggagcgcg
     3541 gactcgatga tcgacggccg cacgatcgac aagggaagct gctcggcggc ggcgagctcc
     3601 tccaccgcgc gctcgcccat cgccttcgtg aacgtgtaca catccggcca tccgagcgac
     3661 cgcgcgcgct gccgcccgta ctccacgagc cgcttggtca tccaatcctt gcgtcgctgc
     3721 tcggcgtcgt ccgcgacggt cgtcggcccc gcgcgggagt gctccttgga cgcatcctcg
     3781 aggaagcgcg cgaggagctc gggcttccgc gaggcggcct ccacgtcccg gcgggccgac
     3841 atcgccaggt cggcttcgag ccgccagtcc acgcgatggt ccagcgtgcg ctcggggatc
     3901 acccccttct gcaccccggc cacgtacgcc gtcgagacgt ggaccagcgc cgggcgcgat
     3961 cctgcgtcaa gcacggcgcc gtagaggttc atcgcgccca gcaggttcgt ctggaaaccc
     4021 tcgtcgatgg gcgggtcgaa cgagaccgtc gcggcggagt ggatcacgac gtcgatgtct
     4081 cccggcaggt ccggccggcc ccgggtgaag tccccgtcga tcacctcgat ccgctcgtcc
     4141 aggagatgga ggagggcgtc gtccgagccc agggactcgc ggagcctgtc gaacgaaggc
     4201 ttccgggtca ggtagtggac acgctccacg gaggaggccc ccgtctggga cctgaccagc
     4261 agcaccaccg tcgtctccgg gaagtcggcg agcagtcgct cgagcacgac ctgccccagg
     4321 aaaccggtga cgccggtcat gaagacccgc ttcccggcca ggcgttcgac gatcgagctc
     4381 gctctcggcg acctcttcgc cttcctcttc gcctcctggg cgcctttgtc taccatccgg
     4441 cagattatcg ctctctaccg ttcggtagac aaccccgctg ctcaaggggg cgttgccgca
     4501 ggtcgaaacg caagagagtc ggaccgccct gcaggagaga gcacccgtgt cccttcgttc
     4561 gaagatgctc gccatgtcga cgatcccctt gatcgtcctc gtcttcgccg tgacctatgc
     4621 ggtgtccgcg cagcgaaccg cgtctcgaac gaacgaggac gtggaccgca ccaccggcgt
     4681 ccgccgcgag ctcgccgaga tccagcacga cctggcgatc gcggagtcca gcgttcgcgg
     4741 atccgtcctc acgggacaag cgaggatgcg ggaggagtac ggcgaggcgg tgatcgagct
     4801 ccggagagac ctggaggatc tcaaggcgag ggtgaccgag cccctgcagc ggaaccgtct
     4861 cgaaccgctc gaggcgctcg tcgaggagcg cctggcgacc ctgcaagggg tcctccgtat
     4921 cgggcgggat cgaacgccgg aggcacagga ccgattggag acgttcttgc tccacgggca
     4981 gacgatcacg aacgcgatcc gaggcctgac cgagcagatg gagctgaccg cctcggagct
     5041 gatggccgaa cggatcgcgg cgcgggacgc ggcgttcgcg cgctcgtacc tgatccaggt
     5101 catcgcgatg cccgccgcca tcttcgtcgc gatgatcctg atggccagct tcacgggcgg
     5161 catcgtcaag cgggtggcca agatgggaga gaacgcctac cgactcgaca agggacagcc
     5221 gctggaccgc cccgactctt ccaacgatga gctcggctcc ctctcgagag cgatcgtccg
     5281 aaccggcgcg cacctgtccg atctgcagga ggacctgcgc cggcacgcga ccgtggacga
     5341 cctcaccggc ctggccaacc ggcgcgggtt cttcgcgctg ggagagcacg agctcctcgt
     5401 cgccgcacgg acccgatcct cggtcgccct gctgttcatc gatgtcgacg gcctgaagcg
     5461 cgtgaacgac gagctcggcc acgtcgccgg cgaccagctg ctcaaggaga gcgcagacgt
     5521 gatccgggag accgtccgag cgtccgacat cgccggccgg ctgggcggcg acgagttctg
     5581 cgtgctcctg atcggcgatc ccgagctgga ccctgatcgg gtggtggagc ggatgcgcga
     5641 caccctggct tcccacaacg c
//
LOCUS       DASUYD010000024         7610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1114885, whole genome shotgun sequence.
ACCESSION   DASUYD010000024 DASUYD010000000
VERSION     DASUYD010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114885"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF195_01235"
     CDS             <1..387
                     /locus_tag="VF195_01235"
                     /inference="COORDINATES: protein motif:HMM:NF014800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579490.1"
                     /translation="DHIGEVKRHNHMTLRIGPEPGASLGVMVKAPGEDRPDPVHLDFS
                     FRELGRAPAPYERLLLDAIDGDGTLFPRQEVIEETWRILQPLLDAPAPIEGYARGSWG
                     PASADRLAHDHGGWRVPEARHLRLSR"
     gene            420..869
                     /locus_tag="VF195_01240"
     CDS             420..869
                     /locus_tag="VF195_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003937200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6579491.1"
                     /translation="MTGRPAAAQHLRDLALLRRVRDRMDREYAQPLDVEALARGVHMS
                     AGHLSRQFRLAYGESPYSYLMTRRIERAMALLRRGDLSVTDVCFEVGCSSLGTFSTRF
                     AELVGMPPRTYRQRAAGAMAGVPPCVAKQVTRPIRNREAPVTEPHVA"
     gene            875..1285
                     /locus_tag="VF195_01245"
     CDS             875..1285
                     /locus_tag="VF195_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009476925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6579492.1"
                     /translation="MDITIHSSFLPQDDPDAALAFYRDVLGFEVRGDVEYGGMHWITV
                     GPPNQPGTAIVLHPPAVTPGITDDERRTIVEMMAKGTYAGINLATKDLDGTFERLQAS
                     DTDIVQEPIDQPYGVRDCAVRDPAGNLLRIQELA"
     gene            1419..1789
                     /locus_tag="VF195_01250"
                     /pseudo
     CDS             1419..1789
                     /locus_tag="VF195_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1801..2268
                     /locus_tag="VF195_01255"
     CDS             1801..2268
                     /locus_tag="VF195_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6579493.1"
                     /translation="MSAKKTTRKAAKSTTTKGKTGTGWSPEERAAAKAYAQELKEEAR
                     GANDEKAALAAIAAMPAKDRAMGKRIHAIIKASAPDLAPKTWYGMPAYAKDGKVICFF
                     QSADKFKARYATFGFNDDANLDAGTMWPTSWALAELTPADEKKIAALVKKAVR"
     gene            2265..4640
                     /locus_tag="VF195_01260"
     CDS             2265..4640
                     /locus_tag="VF195_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6579494.1"
                     /translation="MSPTSSPAPPVADSHDLIRVQGARVNNLKDVTVEIPKRRLTVFT
                     GVSGSGKSSLVFDTIAAESQRMINETYTAFVQAFMPTLARPEVDVLEGLTTAIIVDQE
                     RMGSNPRSTVGTATDANAMLRILFSRLGKPHIGSPNAYSFNVPSARGSGAITVERGAK
                     TKTEKATFTLLGGMCPRCEGKGNVTDIDLTQLYDESKSLAEGAITIPGYTADGWNVRI
                     FSESGFLDADRPIRKYTKKELHAFLYGEPTKVKVNNINLTYEGLIPKIQKSFLSKDVD
                     AMQPHIRAFVERAVTFTTCPECEGTRLAAEARSSKIKGKSIADVCAMQVSDLADWVRD
                     LDEPSVAPLLTGLQHLLDSFSEIGLGYLSLDRPSGTLSGGEAQRTKMIRHLGSSLTDV
                     TYVFDEPTIGLHPHDVRRMNDLLLQLRDKGNTVLVVEHDPEAIAIADHVVDLGPGAGS
                     AGGSVCFEGDVEELRRSDTTTGRHLDDRAILKEEVRQSSGALEIRGATAHNLQGVDVD
                     IPLGVLCVVTGVAGSGKSSLIHGSVAGRDGVVVIDQGTIRGSRRSNPATYTGLLEPIR
                     KAFAKSNGVKPALFSSNSEGACPTCNGAGVIYTELGFMDTVESTCEECEGKRFQASVL
                     EYTLGGRNIAEVLAMPVIEAEAFFDDDEAHTPAAHTILERLADVGLGYLSLGQPLTTL
                     SGGERQRLKLAAQMGDKGDVYVLDEPTTGLHLADVEQLLGLLDRLVDAGRSVIVIEHN
                     QAVMAHADWMVDLGPGAGHDGGRVVFEGTPADLVATGSTLTGEYLAAYVGT"
     gene            complement(4712..5179)
                     /locus_tag="VF195_01265"
     CDS             complement(4712..5179)
                     /locus_tag="VF195_01265"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX6579495.1"
                     /translation="MRRLELLRHAKRDPDEDRLSAAGRAQAEDVGRGTSGYEAVFVSP
                     AARAAETAAWILRGSGRQLTSHGVIDGLAGKDATGGTPEGMAAGIRALLAAVPEGGVG
                     LAVSHTPFVERAMTGLTGREPQPFAECEGIRVTQTKDGRIEVEEIRRPASTKA"
     gene            complement(5186..6394)
                     /locus_tag="VF195_01270"
     CDS             complement(5186..6394)
                     /locus_tag="VF195_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6579496.1"
                     /translation="MTGLFRRRTYLPERELRRAYDVVVVGGGVNGLSLAYHLAAEHGI
                     RDVAVLERSYIGSGGSGRNTQVLRANYNTPETVPLYAASLARYRRLSQELGFNVQFST
                     EGELDLCHTEDSLQVEQEKSALNRALGVQTDILGPDEILEVCPLVDLSGGGELPVVGA
                     SYHPPGSFARHDAVVWGFAQAAARRGVDIHQGVAVTGVQVDHDACVGVETSAGPVAAG
                     HVVSAVAGWSTQLAAMAGVRLPIVTHLLQAFVTEPYRPVLRGLVSSMDLTIYVSQTAR
                     GELLAGAEIVPYSTFSTRSTFEFLSEAARRSIMILPFMAEARAMRQWGGSCDMTPDSS
                     PILGPTPVDAFSVMAGMGTWGFKGAPIFGATMADSIATGRVPDLIAPFALSRFREDRM
                     VPDAASAGTH"
     gene            complement(6391..6783)
                     /locus_tag="VF195_01275"
     CDS             complement(6391..6783)
                     /locus_tag="VF195_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579497.1"
                     /translation="MAELWSVHAAEEACDLAMRAPGAGRVAPDEVIVRGSIAEDLHAA
                     VRTIDADAVIRECSEGWSEIELSGADARSTFARMSDLPLPEGGFVQGDVARVAARVFV
                     EEDTLRVLVRASWGAYLARQVGRGRSGT"
     gene            complement(6776..>7610)
                     /locus_tag="VF195_01280"
     CDS             complement(6776..>7610)
                     /locus_tag="VF195_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6579498.1"
                     /translation="GYVFDDGLVCGLGEEGWYLTSTSGGADRMDAWLRDRADRLGLRV
                     HVIDLTAERGAILVAGPFARALLDELTDAPLETEAFPPLGVRELTVADVACVAIRSGF
                     VGEVAFELHHPRSRGPELWEAIVRQGDGLGIRPHGLDALEILRLEKGHPYVGQDTMPD
                     DTPAKLGLAWAVAPGKTFAASHALERLAETPPDRRLVGLAFDRGGAELRGVPLRSRGR
                     IVGRVTSAGWSPTLERSIGLGWIRREPGGDVPDALRADHVAARVVSTPFLDPEGERLR
                     G"
BASE COUNT         1271 a         2726 c         2555 g         1058 t
ORIGIN      
        1 gaccacatcg gagaggtgaa gcgccacaac cacatgacgc tccggatcgg gcccgagccg
       61 ggagcctcgc tcggcgtgat ggtgaaggca ccgggtgagg atcggcccga tcccgtgcac
      121 ctcgacttct ccttcagaga gctcgggcgg gcgccggcac cgtacgagcg cctgctgctg
      181 gacgcgatcg acggcgacgg cacgctcttc ccccgccagg aggtgatcga ggagacgtgg
      241 cggatcttgc agcctttgct cgatgcaccg gcgccgatcg agggctacgc gagggggtcg
      301 tggggcccgg cgagcgcgga ccgcctcgcc catgatcacg gcgggtggcg ggtcccggag
      361 gcacggcact tgaggctgtc gcgctgacac ctccggcgcg acgtggcacg atctcgtggg
      421 tgaccggcag acccgccgcc gcgcagcacc tgcgcgacct cgcgctgctg cgccgagtcc
      481 gcgaccggat ggaccgcgag tacgcgcagc cgctggacgt cgaggcgctc gcccgcggtg
      541 tgcacatgtc cgccggccac ctcagtcgcc agttccggct ggcctacggc gagtcgccgt
      601 acagctacct gatgacgcgg cgcatcgagc gcgccatggc actgcttcgt cgcggcgatc
      661 tcagcgtcac ggacgtctgc ttcgaggtcg ggtgctcgtc gctgggcacg ttcagcactc
      721 gcttcgcgga actcgtcggg atgccgcctc gcacctatcg gcagcgggcg gcgggcgcta
      781 tggcgggtgt gccgccgtgc gtcgcgaaac aggtgaccag accgatcagg aatcgagaag
      841 cgccggtcac ggagccgcac gtagcatgac cgccatggac atcaccatcc actcgagctt
      901 cctcccgcag gacgatccgg acgccgccct ggccttctac cgcgacgttc tcggcttcga
      961 ggtccgaggc gacgtcgaat acggtgggat gcactggatc acggtcggcc ctcccaacca
     1021 gcccggcacg gcgatcgtcc ttcatccccc cgccgtcacc cctggcatca ccgatgacga
     1081 acgccgcacg atcgtcgaga tgatggcaaa gggcacgtac gccggcatca atctcgcgac
     1141 caaggacctc gacggcacct tcgagcgtct gcaggcgagc gacaccgata tcgtccaaga
     1201 gccgatcgac cagccgtacg gggttcgcga ctgcgccgtt cgcgatcccg caggcaacct
     1261 gctccggatc caggagctgg cctgagccat cccgtggtcg ccggcctccg cggcggaacc
     1321 ggtcaggaat cgagaagcgc aggtcaccga gccacggcta gtttgaaggc cgacggaaca
     1381 gaccgggtcc atcgcgaccc cgacctcagg aggcgaccat gaccggctcg tccaccacgc
     1441 agggcatcaa gacggtgttg cacccgtatc cgacatggcc gcggccaagg cggtgtacgc
     1501 cgccctgctc ggcatcccgc cacagacgga tgactcctac tacgtcggtt tcgaggccga
     1561 ggggcagcac atcgggctgg ttccgggcgg cgggccgcag ggcatgacct caccggtggc
     1621 gtacaggcat gtgtcggaca tcgaggcgaa gctggccgag gtggccgccg cggccgccac
     1681 cctgaaggac ccggcccgcg atgtcggggg cggccgcctg gtggcgacca tcaccgaccc
     1741 cgatggcaac gtgctcgggc tgatccaaga ccagtcagtg agcttgtaga ggagggaacg
     1801 atgagcgcga agaagaccac gcggaaggcc gcgaagagca ccaccacgaa gggcaagacg
     1861 ggtacgggat ggtcgcccga ggagcgagcc gcggccaaag cgtacgccca ggagctgaag
     1921 gaggaagcgc gcggagcgaa cgacgagaag gccgcgctcg cggcgatcgc cgcgatgcct
     1981 gcgaaggatc gcgccatggg caagcgcatc catgcgatca tcaaagccag cgcgcctgac
     2041 cttgcaccga agacctggta cgggatgccc gcgtatgcga aggacggcaa ggtcatctgc
     2101 ttcttccaga gcgcggacaa gttcaaggcg aggtacgcga cgttcgggtt caacgacgac
     2161 gcgaacctcg atgccggcac catgtggccg acctcgtggg cgctggcgga gttgacgccc
     2221 gccgacgaga agaagatcgc cgccctcgtg aagaaggcgg tgaggtgagc cccacgtcgt
     2281 cgcccgcgcc gcccgtcgcc gacagccacg acctgatccg cgtgcagggc gcacgcgtga
     2341 acaacctcaa ggacgtcacc gtcgagatcc cgaagcgccg actgacggtg ttcaccggcg
     2401 tctccggctc tggcaagagc tcgctggtgt tcgacacgat cgccgcggag tcgcagcgga
     2461 tgatcaacga gacctacacc gcgttcgtgc aggcgttcat gccaaccctg gcgaggccgg
     2521 aggtcgacgt gctggagggc ctgacgacgg cgatcatcgt ggaccaggag cgcatgggct
     2581 ccaacccccg ctccaccgtc ggcaccgcca ccgacgccaa cgcgatgctc cgcatcctct
     2641 tcagcaggct cgggaagccg cacatcggct cgcccaacgc gtactcgttc aacgttccct
     2701 cggcgagagg cagcggtgcg atcaccgtcg agcgcggcgc caagacgaag accgagaagg
     2761 cgaccttcac cctgctcggc ggcatgtgcc cgcgatgcga gggcaagggc aacgtgaccg
     2821 acatcgacct gacccagctc tacgacgaaa gcaagtcgct cgccgagggc gcgatcacga
     2881 tccccggcta caccgcggac ggctggaacg tccggatctt cagcgagtcg ggcttcctgg
     2941 acgccgacag gccgatccgc aagtacacga agaaggagct ccacgcattc ctctacggcg
     3001 aaccgacgaa ggtgaaggtc aacaacatca acctgacgta cgaggggctg atcccgaaga
     3061 tccagaagtc gttcctgtcc aaggacgtcg acgcgatgca gccccacatc cgcgccttcg
     3121 ttgagcgggc cgtgaccttc acgacgtgtc ccgagtgcga gggtacccgg ctcgccgctg
     3181 aggcgcggtc gtcgaagatc aaagggaaga gcatcgccga cgtctgcgcg atgcaggtca
     3241 gcgacctggc cgactgggtc cgggacctcg acgagccatc ggtggccccg ctgctcaccg
     3301 ggctgcagca cctcctcgac tcgttctcgg agatcgggct gggctacctc tcgctcgacc
     3361 ggccgtcggg caccctgtcc ggaggtgagg cccagcgcac caagatgatc cgtcacctgg
     3421 gctcgtcgct caccgacgtc acctacgtgt tcgacgagcc cacgatcggc ctgcaccccc
     3481 atgacgtccg gcgcatgaac gacctgctgc tgcagctgcg cgacaagggc aacactgtgc
     3541 tcgtcgtgga gcacgatccg gaggcgatcg cgatcgccga ccacgtcgtc gacctcggcc
     3601 ccggcgccgg ctcggcgggc ggctccgttt gcttcgaggg tgacgtcgag gaactgcgga
     3661 ggagcgacac caccaccggt cggcatctcg acgaccgagc gatcctcaag gaggaggtac
     3721 gacagtcgtc cggcgcgctg gagatccgcg gcgcgacggc ccacaacctg cagggcgtcg
     3781 acgtcgacat cccgctcggg gtcctctgcg ttgtcacggg cgtcgccgga tccggcaaga
     3841 gctcgctgat ccacggctcg gtcgccggcc gtgacggcgt cgtggtcatc gaccagggaa
     3901 cgatccgcgg ctcacgacgg agcaacccgg ccacgtacac cggcctgctc gaaccgatcc
     3961 gcaaggcgtt cgcgaagtcc aacggcgtga agcccgcgct gttcagctcc aactccgagg
     4021 gcgcctgccc cacctgcaac ggtgccggcg tcatctacac cgagctgggc ttcatggaca
     4081 cggtcgaatc gacctgcgag gagtgcgagg ggaagcggtt ccaggcgtcg gtgctggagt
     4141 acacgctagg aggccggaac atcgccgagg tgttggcgat gcccgtgatc gaggccgagg
     4201 ccttcttcga cgacgacgag gcccataccc cggccgccca cacgatcctc gagcggctcg
     4261 ccgacgtcgg gctcggctac ctctcgctgg ggcagccgtt gaccacgctc tccggcggcg
     4321 agcggcagcg gctgaagctg gccgcccaga tgggcgacaa aggggacgtc tacgtcctcg
     4381 acgagccgac cacgggcctc cacctcgccg acgtcgagca gctgctcggc ctgctggacc
     4441 ggctcgtcga cgccggcagg tccgtcatcg tgatcgagca caaccaggcc gtcatggcac
     4501 acgccgactg gatggtagac ctcggcccgg gcgcgggcca cgacggcggc cgggtcgtct
     4561 tcgagggaac acccgccgac ctcgtcgcca ccggttccac gctcacgggt gagtacctcg
     4621 cggcgtatgt cggcacctga tcccgctcgc gatcgtgtct gtatcgcgaa agccgccgcg
     4681 ctcaggtggc tgttcggatc cggcgcgctg tctaggcttt ggtggacgcc ggtcgcctga
     4741 tctcctccac ctcgatacgt ccgtccttcg tctgggtcac gcggatacct tcgcactccg
     4801 cgaacggttg gggctcgcgt cccgtgagtc ccgtcatcgc tcgttcgacg aacggggtgt
     4861 ggctgaccgc gagcccgacg ccgccttccg gcacggctgc cagcaacgcc cggatccccg
     4921 cggccatccc ctccggggtg cctccggtcg cgtccttgcc cgcgagaccg tcgatcaccc
     4981 catgcgaggt cagttgccgg cccgaccccc tcaggatcca cgcggccgtc tccgcggcgc
     5041 gcgcggccgg cgagacgaag acggcctcgt agccgctcgt cccgcgtccc acgtcctcgg
     5101 cctgcgcccg ccccgccgcg gagagccgat cctcgtccgg gtcccgcttc gcgtgccgga
     5161 ggagctccag tcgtcgcatc tcgcctcagt gcgtacccgc ggacgccgcg tccggaacca
     5221 tgcgatcctc gcggaaccgg ctgagcgcga acggcgcgat cagatcgggt acgcgaccgg
     5281 tggcgatcga gtcggccatc gtcgccccga agatcggcgc gcctttgaac ccccatgtcc
     5341 ccatgcccgc catcacactg aacgcgtcca ccggtgtggg gccgaggatc ggcgaggaat
     5401 ccggggtcat gtcgcaggac ccaccccatt gccgcatcgc ccgcgcctcc gccatgaacg
     5461 ggaggatcat gatcgacctg cgcgcggcct cggacaggaa ctcgaaggtc gagcgtgtcg
     5521 agaacgtgga gtacggcacg atctcggcac ccgccagcaa ctccccgcgc gccgtctgcg
     5581 acacgtagat cgtgagatcc atcgacgaga ccagcccgcg cagcacgggg cgatagggct
     5641 cggtgacgaa cgcctggagc aggtgcgtca cgatgggcaa gcggacgccc gccatcgcgg
     5701 cgagctgcgt cgaccacccg gcgacggcgc tcacgacgtg gccggccgcg accggcccgg
     5761 cggaggtctc gacgccgacg cacgcgtcgt ggtcgacctg cacccccgtg accgcgacgc
     5821 cctgatggat gtcgaccccc cgacgggcgg cggcctgcgc gaacccccag acgaccgcgt
     5881 cgtgtcgggc gaaagagccg ggtgggtggt acgacgcacc caccacgggc agctcgccac
     5941 cgccgctcag gtccaccaga ggacacacct cgaggatctc gtccggtccg aggatgtcgg
     6001 tctgcacgcc gagtgcccgg ttcagggcgg acttctcctg ctcgacctgc agggagtcct
     6061 ccgtgtggca gagatcgagc tctccctcgg tgctgaattg gacgttgaat ccgagctcct
     6121 gcgacaaccg ccggtagcgg gcgaggctgg ccgcgtacag cgggaccgtc tccggcgtgt
     6181 tgtagttcgc gcgcaggacc tgcgtgttcc ggccgctccc gccgctgccg atgtaggaac
     6241 gctcgagcac ggcgacatcc cggatgccgt gttcggcggc gaggtgatag gcgagcgaca
     6301 gcccgttcac cccgcctccg acgaccacga cgtcgtaggc gcgtcgcagc tcgcgctccg
     6361 gcagatacgt ccggcgacgg aacaggcccg tcacgttccc gatcgccctc tcccgacctg
     6421 tcgcgcgaga taggcgcccc aggaggcccg gacgagcacg cggagtgtgt cctcctccac
     6481 gaacacccgg gccgcgacgc gcgcgacatc cccctggacg aagccgccct ccgggagcgg
     6541 gagatccgac atgcgggcga acgtggagcg ggcatccgcg ccggagagct cgatctccga
     6601 ccatccctcc gagcactcgc ggatgacggc gtcggcgtcg atcgtgcgca cggccgcatg
     6661 gagatcttcc gcgatcgagc cacgcacgat cacctcgtcc ggagcgactc ggccggcgcc
     6721 cggcgcccgc atcgcgaggt cgcatgcctc ctccgcagcg tggacggacc agagctcagc
     6781 cacggaggcg ctctccttcg ggatcgagga acggggtcga gacgacgcgg gcggcgacgt
     6841 gatcggcgcg gagcgcgtcg gggacgtcgc cgcccggttc ccgacggatc catccgagtc
     6901 cgatcgaacg ttcgagggtc gggctccagc ccgccgacgt caccctcccg acgatgcgcc
     6961 cccttgagcg cagtggaacc ccacggagct ccgccccacc gcggtcgaac gcgaggccga
     7021 cgagccgacg gtccggcggc gtctccgcca gccgctcgag cgcgtgcgac gcggcgaacg
     7081 tcttcccggg agccaccgcc cacgcgagtc cgagcttcgc gggggtgtcg tccggcatcg
     7141 tgtcctgccc gacgtaggga tgtcccttct cgagtcgcag gatctcgagg gcgtccagtc
     7201 cgtgcgggcg gatcccgaga ccgtccccct ggcgcacgat cgcctcccag agctccggac
     7261 cacgcgagcg cggatggtgg agctcgaagg caacctcgcc cacgaaaccg ctgcggatcg
     7321 cgacgcaggc aacgtccgcc acggtcagct cccggacgcc cagcggcggg aacgcctccg
     7381 tctcgagcgg cgcgtccgtg agctcgtcga ggagcgctcg cgcgaacggc cccgccacga
     7441 ggatcgcgcc tcgttcggcc gtgaggtcga tcacgtggac ccgcagcccc agccgatcgg
     7501 cgcggtcgcg gagccacgcg tccatccgat cggccccgcc cgaggtcgac gtgaggtacc
     7561 aaccctcctc gcccagcccg cagacgagcc cgtcgtcgaa gacgtagccc
//
LOCUS       DASUYD010000025         6312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1335227, whole genome shotgun sequence.
ACCESSION   DASUYD010000025 DASUYD010000000
VERSION     DASUYD010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6312
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1335227"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VF195_01285"
     CDS             complement(<1..978)
                     /locus_tag="VF195_01285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6579499.1"
                     /translation="MSTYFGFGLITCQRYPGDGRSDVELYAEAIVLAEEAEGLGYDSV
                     WTSEHHFVDDGYLPSLLPLSAAIAAKTERVAIGTGLLLAPLHEPIRVAEDAAVVDLIS
                     KGRFILGIGLGWREEEFEGLHVPISERVSRLEDSIAVYRQAWRGELVTGGERLHFPNV
                     PIRPVPARPGGPRIWIGAVSEPAIRRAGRLADGFMASEVTPSTFAEQVTIAREERVAA
                     GVEDDLAISLHLPTFAWNGDDAWERVRDHHHYVAWKYEDMDRARSRVGPPQPPPPLTQ
                     EKEAGLREQIVLGRPDEVAERIGELRDAAGSDLHYIARLYWPGMDPGVQR"
     gene            complement(975..2147)
                     /locus_tag="VF195_01290"
     CDS             complement(975..2147)
                     /locus_tag="VF195_01290"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX6579500.1"
                     /translation="MDPYPHVKVAGNPRERGRGYGEQARERIERSIEAYGQVFLAYAG
                     WDWEQVRREAARYEAPIAAFDGRYVDEIRGIAEGAGVDVEDVLSINVRTEVMFAAKAR
                     AAKAASRTGGDGRRQGECSAFTVLPEASAEGHTLLGQNWDWLLHCFDTVVVLEATQDE
                     GPNFVSIVEAGLLAKTGLNSSGLGVATNALVTDDDRGEPAIPYHVLLRAFMDCENLSD
                     ALAIAQRGIRSSSANYLLAHADGMAVDIEAAPGDYSRLFLGYPDDGVVLHTNHFRSPA
                     FDGRDVSLWVMPDSPFRLERLNATVRHARPDGLSLDTFRAVLGDHANYPSGICCHPDS
                     RMDPHDQAATVASVLMDLTERRIWLANGHPCTAPYRELDVGALLDKPSPVKEGMPA"
     gene            complement(2161..2913)
                     /locus_tag="VF195_01295"
     CDS             complement(2161..2913)
                     /locus_tag="VF195_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6579501.1"
                     /translation="MTSDRFDGRRALVTGGGSGIGRATAALLAERGARVAVLDHRQDA
                     VRETATAINAVGVTGDVRDTASVHFAVSKATDELGGAIDVLVNAAGIYRVTPAGELAA
                     DEWDDVLNTNLRGPFLVSRAVMQGDPADGWRAIVNVASTAAFVGDAGEPAAHYNASKS
                     GVVALTKQLAIEWATRRTRVNAVAPGVIDTPMLRLMDVPDAGSRYLDTRVPLRRLGRA
                     QEVAEVIAFLASDRAAYVTGATIPVDGGVTAL"
     gene            complement(2910..3677)
                     /locus_tag="VF195_01300"
     CDS             complement(2910..3677)
                     /locus_tag="VF195_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6579502.1"
                     /translation="MKCVVTGAARGLGERIAARLVSDGGHVALIDVDQGVNATAERLQ
                     AEHPGSLALAVRCDVSDEAGVASAVGEVAAQLGGIDLLVNNAGIGGPSTTVMDTSLEE
                     FRHVLDVNLIGTFLMARACVRVMAEGGGGCIVNIGSILGQQGTANGAAYCASKGGVAL
                     FTHSLALEVAHLGIRVNTIAPGNMLTEMHLADLRERAADRDARVEAEVDAVRATVPLD
                     RHGTGDDVAGAVAWLASPDASYVTGQTIGVNGGVLLT"
     gene            complement(3674..4462)
                     /locus_tag="VF195_01305"
     CDS             complement(3674..4462)
                     /locus_tag="VF195_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6579503.1"
                     /translation="MTDWPNDARAAVAFTFDFDAEEVWIGEDPANADRPGILSQGTYG
                     AKVAVPLVLELLRRRELRATFFIPGRVAERHPGRVDEIAAAGHEIAHHGYTHTSPTKL
                     SREEEEAELVKGREVLEGFGVEVRGYRSPSWDFSEHTLSLLETHGFTYSSNLMDDIRP
                     YRHNGSSLVEVPIQWILDDAPHFWFDPSTWTKKISTVEEVRSIWEHEVLGIIDLGGAA
                     ILTCHPQIIARPGRVGFLEEFIAFVQGLDEVWVATTGEIAELAT"
     gene            complement(4459..5547)
                     /locus_tag="VF195_01310"
     CDS             complement(4459..5547)
                     /locus_tag="VF195_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6579504.1"
                     /translation="MGVAARGNLVDDLGWRRPNPVGVTAVITLAALLPIIWPNDYVID
                     VGLTALIWMILNQSWNLQLGIGGIWNFGQLAIFAIGAYSSALVSIHWGVPGWLAMLIG
                     GLGAAIASLIIAIPVLRLRGIYASLLTFSFAEVVRLLVISDQSGLTGGTFGLSGVPDL
                     SFGGLTPDARQRAYYWLALALVVATAVVIYVFIHSPLGVSLKALRDSPGYAAARGVSP
                     MRMQVITFTTAAFIAGIAGGFYAHYFGVVSPSVMGLGPMALFVTMLVIGGLGTFAGPM
                     VGTALITVVSEYLRDYQEARLIILGLILLVTVVVAPRGLVPVLQDARARVQRWMSEDE
                     PSNDAETGTGSETDESSELPVSSSKGER"
     gene            complement(5547..>6312)
                     /locus_tag="VF195_01315"
     CDS             complement(5547..>6312)
                     /locus_tag="VF195_01315"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6579505.1"
                     /translation="MVMIGGYVAWMVFDGLGWGPILGGVAAAVVGFAFGVAIQQAAIR
                     PLIGRRGVDFEMTAFISTFAVAIVLSNVALQIYGARSKAVPPVIHGRLHLLEGISVSW
                     HSLVVAGIAVGTLLALGAFLSKTRYGLAISAVAQQLDAARLMGIPAQRVYNLTMGVSS
                     ALAAIAGVLLASIFFVSPNAGDLPLLKALIVAIFGGLGSVRGTILAAYIIGLAEALVS
                     LYLGVKWSLPLLFLSIMIVMLVRPTGLLGKPEEARL"
BASE COUNT         1063 a         2259 c         2056 g          934 t
ORIGIN      
        1 tcgctggacg ccggggtcca tccccggcca gtacagccgt gcgatgtagt ggaggtcgct
       61 gcccgcggcg tctctgagct ccccgatccg ctcggcaacc tcgtccggcc tcccgaggac
      121 gatctgctcg cggagcccgg cctccttctc ctgcgtgagc ggcggaggag gttgtggcgg
      181 gcctacacgg ctccgcgctc ggtccatgtc ttcgtacttc cacgccacgt agtggtggtg
      241 gtcgcggacg cgctcccacg cgtcatcgcc gttccaagcg aacgtcggca ggtgcagcga
      301 gatcgccagg tcgtcctcga cgccggccgc gacgcgctcc tcgcgcgcga tggtcacctg
      361 ctcagcgaag gtcgacggcg tgacctcgga ggccatgaac ccgtcggcca gccggcccgc
      421 gcggcggatc gcgggttcgc tcaccgcgcc gatccagatc cgtggtccac ccggcctcgc
      481 ggggacaggt cggatgggca cgttcgggaa gtgcagccgc tcgcctccgg tgacgagctc
      541 gcctcgccat gcctggcgat agaccgcgat cgagtcctcg agtcgcgaca cgcgctcgga
      601 gatgggcaca tggaggccct cgaactcctc ctcgcgccaa ccgagcccga tgccgaggat
      661 gaaccgcccc ttcgagatca ggtccacgac ggccgcatcc tcggcgaccc ggatcggctc
      721 gtgcagcggc gcgaggagca gcccggtccc gatcgcgacg cgttcggtct tggccgcgat
      781 cgcggcgctc agtggtagca gcgagggcag gtagccgtcg tccacgaagt ggtgctcgga
      841 ggtccaaacc gagtcgtagc ccagaccctc ggcctcctcg gcgaggacga tcgcttcggc
      901 gtacagctcg acgtcgctcc gcccgtctcc ggggtaccgc tggcacgtaa tcaggccgaa
      961 gccgaaatac gtgctcacgc gggcatgccc tctttgaccg gactcggctt gtcgagcaga
     1021 gcgccgacgt cgagctcgcg gtagggcgcg gtgcaggggt gcccgttcgc gagccagatc
     1081 cgccgctcgg tcagatccat cagcaccgat gcgaccgtcg cggcctgatc gtgtggatcc
     1141 atgcgggagt cgggatggca gcagatccca gaggggtagt tggcgtggtc gccgagcacg
     1201 gcgcggaacg tgtcgagcga gagcccgtcc ggacgggcgt gccggaccgt ggcgttcagt
     1261 cgctcgaggc ggaacgggct gtccggcatc acccagagcg agacgtcccg cccgtcgaac
     1321 gccggtgacc ggaagtgatt cgtgtggagc accacaccgt cgtcggggta tccgaggaag
     1381 agccgggagt agtcaccggg cgccgcctcg atgtcgacgg ccatgccatc ggcgtgcgcg
     1441 aggaggtagt tcgccgagga ggagcggatc ccgcgctggg cgatcgcgag ggcatccgac
     1501 aggttctcgc aatccatgaa cgcgcggagc agcacgtggt atgggatcgc cggttcgccg
     1561 cgatcgtcgt cggtcacgag tgcgttcgtc gccaccccga gtcccgagga gttgaggccg
     1621 gtcttcgcga gcagcccggc ctccacgatg ctgacgaagt tcggtccctc gtcttgtgtg
     1681 gcctcgagga ctaccaccgt atcgaagcag tgcaggagcc agtcccagtt ttgaccgagg
     1741 agcgtgtgac cctcggcgct cgcctcgggg agcacggtga acgcgctgca ctcaccctgc
     1801 cgccgcccgt cgccgccggt acgggaggcg gccttggcgg cgcgggcctt cgcggcgaac
     1861 atcacctccg tccggacgtt gatcgacagg acgtcctcga cgtcgacgcc ggcgccctcg
     1921 gcgatcccgc ggatctcatc gacgtagcgc ccgtcgaagg ccgcgatcgg cgcctcatat
     1981 cgcgccgcct cgcgccgaac ctgctcccag tcccaacccg catatgcgag gaacacctgg
     2041 ccgtacgcct cgatcgatcg ctcgatccgc tcgcgcgcct gctcgccgta tccgcggccg
     2101 cgttcgcgcg ggttccccgc gaccttcacg tgcgggtacg ggtccatggc agctccccct
     2161 tcacaacgcc gtcacccctc cgtccactgg gatcgtcgct cccgtcacgt acgcggcgcg
     2221 gtccgacgcc aggaacgcga tcacctcggc gacttcctgg gcacgaccga gccgccggag
     2281 cggcacccgt gtgtccaggt accggctgcc cgcgtccggg acatccatca acctgagcat
     2341 cggggtgtcg atgaccccgg gcgcgaccgc attcacacgg gtgcgtcgcg tcgcccactc
     2401 gatcgccagc tgcttcgtga gcgcgacgac gcccgacttg ctcgcgttgt agtgcgcagc
     2461 cggctcgccg gcgtcgccga cgaacgcggc ggtcgatgca acgttgacga tcgctcgcca
     2521 gccgtccgcc ggatcgcctt gcatcaccgc ccgagagacg aggaacgggc cacgcaggtt
     2581 cgtgttgagg acgtcgtccc actcgtcggc cgcgagttcc ccggccgggg tcactcggta
     2641 gatgccggcg gcgttcacca gcacgtcgat cgcacccccg agctcatcgg tggctttcga
     2701 gacggcgaag tgaacgctcg ccgtgtcccg cacgtcgccc gtgacgccca ccgcgttgat
     2761 cgcggtcgct gtctcgcgca cggcatcctg gcggtggtcg agaacggcga cccgcgctcc
     2821 tcgctcggcg aggagcgctg ccgtcgctcg gccgatgccc gagccgcccc cggttacgag
     2881 cgcccggcgg ccgtcgaacc gatcgctcgt cacgtcagca gcaccccacc gttgacgccg
     2941 atcgtctggc ccgtcacgta agaggcatcc ggggaggcga gccacgccac cgctccggcg
     3001 acgtcgtcac ccgtcccgtg ccgatcgagg ggaaccgtcg cgcggaccgc atcgacttcc
     3061 gcctcgacgc gcgcgtctcg gtcggcagcc cgctcgcgca ggtctgccag atgcatctcc
     3121 gtcagcatgt tgcccggcgc gatcgtgttg acgcggatgc cgaggtgtgc gacctccagc
     3181 gcgagcgagt gcgtgaacag cgcgacgccg cccttggacg cgcagtacgc cgcgccgttc
     3241 gccgtgccct gctgtccgag gatcgagccg atgttcacga tgcaaccgcc tccaccctcg
     3301 gccatcacgc gcacgcaggc ccgggccatg aggaacgtgc cgatgaggtt gacgtccagc
     3361 acgtgacgaa actcctcaag ggaggtgtcc atgacggtgg tcgacggtcc gccgatcccg
     3421 gcgttgttca cgagcagatc gatcccgccg agctgcgccg cgacctcgcc cacggcggac
     3481 gcgacgccgg cctcgtccga gacgtcacac cgcacggcaa gcgcgagcga tcccgggtgc
     3541 tccgcctgca accgctccgc cgtcgcgttc acgccctggt cgacatcgat cagcgcgacg
     3601 tgcccaccgt cggacacgag ccgtgccgcg atgcgctcgc cgagcccgcg agcggcgccc
     3661 gtcacgacgc acttcacgtg gcgagctccg cgatctcgcc ggtcgtcgcg acccacacct
     3721 catcgagacc ctgaacgaac gcgatgaact cctcgaggaa acccacgcgc cccggcctcg
     3781 cgatgatctg cgggtggcac gtcaggatcg ccgcgccccc gagatcgatg atgcccagca
     3841 cctcgtgctc ccagatcgac cgaacctcct cgaccgtcga gatcttcttc gtccaggtcg
     3901 aggggtcgaa ccagaagtgc ggcgcgtcgt cgaggatcca ttggatcggc acctcgacca
     3961 gcgacgagcc gttgtggcgg tatggacgga tgtcgtccat gaggttcgac gagtaggtga
     4021 acccgtgggt ctccagtagc gacagggtgt gctcgctgaa gtcccacgac ggcgaccggt
     4081 agccacggac ctcgacgccg aaaccttcca ggacctcccg ccccttcacg agctccgcct
     4141 cctcctcttc ccgtgagagt ttcgtcggcg aggtgtgcgt gtagccatga tgggcgatct
     4201 catggccggc ggcggcgatc tcgtccacac ggccggggtg acgctccgcg acccggcccg
     4261 ggatgaagaa ggtcgcgcgt agctcgcgcc gacgcagcaa ttcgaggacg aggggcaccg
     4321 ccaccttggc tccgtaggtg ccttgcgaca ggatgccggg acggtcggcg ttcgccggat
     4381 cctcgccgat ccagacctcc tcggcgtcga agtcgaaggt gaacgccacg gcggcgcgcg
     4441 cgtcgttcgg ccagtcagtc atcgttcccc cttgctgctg ctcacgggca actcagatga
     4501 ctcgtccgtc tccgatcccg tccctgtctc cgcgtcgttg gagggctcgt cctccgacat
     4561 ccaacgctgt acgcgggctc gcgcgtcttg caggactggc acgagtcccc gcggcgcgac
     4621 gacgacggtc accaagagga tgagacccag gataatcaga cgagcctcct ggtagtcccg
     4681 caggtactca gacacgaccg tgatcaaagc ggtgccgacc atcggtccgg cgaacgtgcc
     4741 gaggccaccg atcacgagca tcgtgacgaa caacgccatg ggccccagac ccatcacgct
     4801 gggcgagacc acgccgaagt agtgagcgta gaagccgccc gcgatgcccg cgatgaacgc
     4861 tgccgtggtg aacgtgatca cctgcatgcg catcgggctc accccgcgag ccgctgcata
     4921 ccccggcgag tcgcgaagcg ccttcagcga aacaccgagc ggcgagtgaa tgaacacgta
     4981 gatgacgacc gcggtcgcca caaccagcgc taatgcgagc caatagtagg cgcgctgcct
     5041 cgcatcgggc gtgagtccgc cgaaggagag gtccggcacg cccgaaaggc cgaacgtccc
     5101 gcccgtcagg ccgctttggt cggagatcac cagcagccgc accacctcgg cgaaggagaa
     5161 cgtcaacagc gacgcataga tgccgcgcag acgcagcacc ggtatggcga tgatcagact
     5221 ggcgatggcg gccccgaggc ccccgatcag catcgcaagc caccccggca cgccccagtg
     5281 gatgctgacg agcgcggacg agtaggcgcc gatcgcaaag atggccagct ggccgaagtt
     5341 ccagatgccc ccgatgccga gctggagatt ccacgactgg ttcaggatca tccagatcag
     5401 cgccgtcaac ccgacgtcaa tcacgtagtc gttcggccag atgatcggca gcagggccgc
     5461 gagcgtgatc accgcggtga cgcccactgg attcggtcgc cgccagccga gatcgtcgac
     5521 gaggttgcca cgggcggcca cacccatcac agcctcgcct cttccggctt gcccaacagc
     5581 ccggtgggac gcaccagcat cacgatcatg atcgacagga acagcagggg cagcgaccac
     5641 ttcacgccca agtacaggga cacgagcgcc tcagccaatc cgatgatgta agcagcgagg
     5701 atcgtgccgc gcacgctgcc gagcccgccg aagatcgcga cgatcagggc cttcagtaac
     5761 ggcagatcgc cggcgttcgg cgacacgaag aaaatcgacg ccagcagtac acccgcgatc
     5821 gccgcgagcg ccgacgagac ccccatggtc aggttgtaga cgcgttgcgc ggggatgccc
     5881 atcagacgag cggcgtccaa ctgctgggcc accgcgctga tcgcgagccc gtatctcgtc
     5941 ttcgagagga acgcgccgag cgccaacagc gtccccacgg cgatcccggc caccaccagc
     6001 gaatgccaac tcacgctgat gccctcgagc aggtgcaggc gcccatgaat caccggaggc
     6061 acggccttgc tgcgagcgcc gtagatctgg agcgcgacgt tcgacaggac gatcgcgacg
     6121 gcgaacgtcg agatgaaggc cgtcatctcg aagtccacgc cgcgacgccc gatcagggga
     6181 cggatcgcag cctgctgaat cgcgaccccg aaggcgaacc ccacgacggc cgccgcgacc
     6241 cctccgagga tgggtcccca ccccaagccg tcgaacacca tccaggcaac gtagccaccg
     6301 atcatgacca tc
//
LOCUS       DASUYD010000026         6161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1115383, whole genome shotgun sequence.
ACCESSION   DASUYD010000026 DASUYD010000000
VERSION     DASUYD010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115383"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..643)
                     /locus_tag="VF195_01320"
                     /pseudo
     CDS             complement(1..643)
                     /locus_tag="VF195_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016579326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(689..1165)
                     /locus_tag="VF195_01325"
     CDS             complement(689..1165)
                     /locus_tag="VF195_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579506.1"
                     /translation="MFIRYYLELQLPFDEVERALLADPASWLPGMATEAEDRGERLLV
                     EVGFEVGDDRRIEREVRIEVGEPVSVPSTTTTLPITWEAKHGARAFPQLDADIEIAAL
                     GARRTQLSMSARYRPPLGMVGRTLDKALMHRVAEATLKDFLDRVGERLALRAGAHR"
     gene            1276..1707
                     /locus_tag="VF195_01330"
     CDS             1276..1707
                     /locus_tag="VF195_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579507.1"
                     /translation="MEEYGSRGTVLLVESDAQERERLATGLEDAGFNVTLCPGPSGPD
                     YTCVGARTLECPLAKGASVVVLDMWLEGDDLMEGTPAEELLDVYLLAGHRVIALASHL
                     PEETPGRVVRLPRRPGSEELATAVGSLAPSLGWTTPRTHRT"
     gene            1722..3062
                     /locus_tag="VF195_01335"
     CDS             1722..3062
                     /locus_tag="VF195_01335"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6579508.1"
                     /translation="MSSGLRWHVALAAASILMLAASGCTSAGEEAPGEGAASAPATFQ
                     VSLTEFAISPATIHAPEGEALTFEVTNEGTAPHTLAVDTGEGVQATAEIPPAESATLE
                     VSALQAGTYETFCTISGHREAGMVGLLMVQQGGVAAAGSTGSVGETGGTVSTEEMLDG
                     HEAGVAAFPAETEGVGNEPLEPRIENGRKVFDLTATEVTWETSPGMFVDAMAYNGTVP
                     GPEIRVRQGDHVLIIVHNEMTQPTIMHFHGLTVPNDMDGVPFITQDPILPGGFFVYEF
                     EVVDPPGMYVYHSHFNSTEQVGKGLYGAFFVQPRRDDWGSLYGTDIAVEHTMFLGDGP
                     TGFVLNGKEFPATQPIVATLGDNVLIHLSNDGSQIHPMHLHGYHFQVVAEDGFVLDPA
                     NRYMADTLAVAPGQRFDILVKAEYAGVWAYHCHILPHVEGPHGMYGMVTALIVQ"
     gene            2990..3532
                     /locus_tag="VF195_01340"
     CDS             2990..3532
                     /locus_tag="VF195_01340"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEX6579509.1"
                     /translation="MPHPSARRGPARDVRDGDGADRAVGPYSGSSTRTVVPGDGLEVG
                     ATALSTAAAKTSLPFLVVLVRGILANALVCLAVWLAAAGRSVIDKVAAIVFPISAFDA
                     AGFEHSVANMCFIPMGLALREQPDVVEAAHLPSAGLSTLNGAGLLNNLVAATIGNAIG
                     GAVLVGLVYWFVYVRPARPS"
     gene            complement(3549..4247)
                     /locus_tag="VF195_01345"
     CDS             complement(3549..4247)
                     /locus_tag="VF195_01345"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEX6579510.1"
                     /translation="MMRSHERIEELIAFRSIYGLDPQEQAELEREMASHPPDCEECRR
                     LETEYEEVGGRLAFALEPVALRPGFADETIELAIGEPPGSHRKAGSRLRPLVAVAAAL
                     VLFVGGAIVGAAVFDGAEVSQQATVLQLEPQDPALSGSITAAFTPGERGIYLQGAGLE
                     TLPQDRVYELWVITGDTPAAAVCVRPGEDGSVFAFADSQVAPDDLLAVTVEPSACSEA
                     PTTPPIWVAPVTLT"
     gene            complement(4379..5305)
                     /locus_tag="VF195_01350"
     CDS             complement(4379..5305)
                     /locus_tag="VF195_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6579511.1"
                     /translation="MRLYVQGLPSYRVLSVLLEQRLGRRVGRFTLNRWVDELGARAKT
                     PVEVSAELGPTSWSGFLGVDGKAIFVAGDEAALLVGVDQGTHDVVHAVVADIEGEEAF
                     ERLVREAVSKGGYPLRGLVSDASPPFLAAHANYLARAPLQLCRIHASRRLDFTIPKAK
                     RSPDAPLRAELKDRVRAVLFATTEEEARRLFGDLLADRDRYQGLGRSDTIGAMERSFE
                     LYMTHHRVKGMPADANITENVIKQLGKKLRLMEGFASMESAEQFSRLLVGCYRFKRFT
                     DSCRRNDNGKSPLQLAGVDTKATDWLRFLLRR"
     gene            complement(5603..>6161)
                     /locus_tag="VF195_01355"
     CDS             complement(5603..>6161)
                     /locus_tag="VF195_01355"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579512.1"
                     /translation="TVAPSSLLEALDARASREVSVTADLSDARDRELVQRIRRGDGEA
                     FRGLFGRYAPSAMALARRVVRQPFLAEEIVQEAFLAVWRNPGGYDPQRGSVRAWLMGM
                     VHHRAVDTVRREESQRRRVEESQLSDPVVQPDPAEDIVDEIGLPEERRAVRAALDELP
                     AEQRQVIELMYFDGLSQSKIARASS"
BASE COUNT          962 a         2117 c         2115 g          967 t
ORIGIN      
        1 tcagccctgc gtgcccgggg tggtcgtatg gcgggccagg taggacgcgg cctcggcccg
       61 ccgcgcgacc tcgagcttgg acaggatcct cgagacgtag ttcttcaccg tctctcggcc
      121 aggccgagct cgtcgccgat ctccctgttc gttttgccgt cggcgacgag cgagaggatc
      181 cgctcctcct gaggcgacag gcgggccagc ctctcgtcct tcagcaggtg cttccccttg
      241 cgcaaccggt cgaggaccgc gctcgtgacc gccggctcca gcagactctc gcccttgccg
      301 accgtgcgga tcgcgcgaac gagctcgccg ccgcgaacct gcttgagcac gtagccagac
      361 gcgccggcca tgatcggggc gaacacggcc tcgtcgtcgg cgaacgaggt cagcatcacg
      421 acccgggtct tcgggtggtt ggcccggatc tcgcgggtag cctctatccc gctcccatcg
      481 gccaggcgga cgtccctgac caccacgtcc ggcctgggtc cggtcggcct catcgacgga
      541 ctctcgaacg gttcccgcct cgccggacac cacgatgtcg tggcgtggcc tgcaggagag
      601 ctttgacacc actgcggacg acctcgtgat cgtcgacgag caggacacgt agcggggctg
      661 gttcgcggac gccatcggca gagggatact agcggtgagc gccggcccga agggcgaggc
      721 gttcgccgac gcggtcgagg aagtccttca gcgtcgcctc ggctacccgg tgcatcaagg
      781 ccttgtcgag cgtgcgtcct accatcccta aggggggacg gtagcgagcg ctcatcgaca
      841 gctgcgtgcg cctggctccg agcgccgcga tctcgatgtc ggcgtcgagt tgtgggaacg
      901 cgcgggcacc gtgtttcgcc tcccacgtga tcggcagcgt cgtcgtggtc gatgggaccg
      961 agacgggctc gccgacctcg atccggacct cccgttcgat ccggcggtcg tcacccacct
     1021 cgaaccccac ctcgacgagc aggcgctcgc cccgatcctc ggcttcggtc gccatgccgg
     1081 gcagccacga tgccgggtcg gcgaggagcg cccgctccac ctcgtcgaac gggagctgca
     1141 actcgaggta gtacctgatg aacatcgaga acgaggatgc ggctgccccg gtgcacccag
     1201 gagggccgaa ggacctccga tgggggggac ggagggccca aggcgagacc gcggctccac
     1261 cggagactgc gacacatgga ggagtacggg tctcgcggga cggtcctgct cgtggagtcc
     1321 gacgcgcaag agcgcgagcg gctcgccaca ggtctcgagg acgccgggtt caacgtcacg
     1381 ctgtgcccag gcccatcggg gcccgactac acgtgcgtcg gcgcccgaac cctggagtgt
     1441 ccgctcgcga agggggcctc ggtcgtggtc ctcgacatgt ggctcgaggg tgatgacctg
     1501 atggagggaa cgccggctga ggagctcctc gacgtctacc tcctcgccgg ccatcgagtg
     1561 atcgccctgg cctcgcacct ccccgaagag acccccggcc gggtggtccg gttgccccga
     1621 cgcccggggt ccgaagagct ggccacggcg gttggctcac ttgccccgtc cctggggtgg
     1681 acgactccta gaacccaccg cacgtgaacg aggaggaaga catgtccagc ggtttgcgat
     1741 ggcacgtggc gctcgccgcg gcctcgatcc tgatgctcgc ggcgagcggc tgcacctcgg
     1801 ccggcgagga agccccgggg gagggtgccg catccgcgcc ggcgaccttc caggtctcgc
     1861 tcacggagtt cgcgatcagt ccggccacga tccacgcgcc ggagggtgaa gcgctgacgt
     1921 tcgaggtcac gaacgagggg acggcgccgc ataccctcgc ggtcgatacc ggcgaagggg
     1981 tgcaggcgac ggcagagatc ccgccagcgg aaagcgcaac gctcgaggtc tcggcgttgc
     2041 aggccgggac ctacgagaca ttctgcacga tctcaggcca cagggaagcc ggcatggtgg
     2101 gcttgctgat ggttcaacag ggtggagtag ccgcagccgg atcgacgggc tcggtgggcg
     2161 agaccggcgg caccgtgagc accgaagaga tgctcgacgg acatgaggcc ggcgttgcgg
     2221 cttttccggc ggagaccgag ggggtcggga acgagccgct cgagcctcgg atcgagaacg
     2281 gcaggaaggt cttcgacctc acggcgaccg aggtgacgtg ggagacctcg cccggcatgt
     2341 tcgtggacgc gatggcgtac aacggaaccg ttccgggccc cgagatccgt gtgcgccagg
     2401 gcgaccacgt tttgatcatc gtgcacaacg agatgacaca gccgacgatc atgcacttcc
     2461 acggcctgac cgttcccaac gatatggacg gcgtgccgtt catcacgcag gacccgatcc
     2521 tgcccggcgg gttcttcgtg tacgagttcg aggtcgtgga cccgcccggc atgtatgtct
     2581 accactcgca tttcaactcg acggagcagg tcggtaaggg tctatacggg gccttcttcg
     2641 tccagcctag gagggatgac tggggttccc tctacgggac cgacatcgcc gtcgagcaca
     2701 cgatgttcct gggggacggg ccgacgggct tcgtgctgaa cggcaaggag ttcccggcga
     2761 cccagccgat cgtcgcgacg ctcggcgaca acgtcctgat ccacctgtcg aacgacggtt
     2821 cgcagatcca tccgatgcac ctccacggct accacttcca ggtcgtcgcg gaggacgggt
     2881 tcgtgctcga tcctgcgaac cggtacatgg cggatacgct ggccgtcgct ccgggtcagc
     2941 gcttcgacat cctggtgaag gccgagtatg ccggcgtctg ggcgtaccac tgccacatcc
     3001 ttccgcacgt cgagggcccg cacgggatgt acgggatggt gacggcgctg atcgtgcagt
     3061 aggcccctac agcggcagct cgacccggac cgtcgttcct ggagacggcc tcgaagtcgg
     3121 cgcgacggcg ctctccacag ccgccgccaa gacctcgttg ccgttcctcg tggttctggt
     3181 ccgaggcatc ctcgcgaacg cgctggtctg tcttgccgtc tggctcgccg cggcgggccg
     3241 ttcggtgatc gacaaggtcg cggcgatcgt cttcccgatc tctgcgttcg acgcggccgg
     3301 cttcgaacac agcgtcgcca acatgtgctt catccccatg gggctcgcgc ttcgagagca
     3361 gcccgacgtg gtggaagcgg cgcacctccc gagtgccggg ctttcgaccc tcaacggcgc
     3421 tggcctcctg aacaacctgg tcgccgcgac gatcggcaac gcgatcggtg gggcggtgct
     3481 tgtgggcctc gtgtactggt tcgtctacgt ccgcccggca cgcccgtcgt gatgtcgagg
     3541 aacgacggtt acgtcaacgt gaccggcgcg acccagatcg gcggcgtggt cggggcttcc
     3601 gagcacgcgg acggctcgac cgtgacggcg agcaggtcgt ccggcgcgac ctgcgagtcg
     3661 gcgaacgcga acaccgaccc gtcctcgccg ggccggacgc agaccgcggc ggcgggggtg
     3721 tcgcccgtga tcacccagag ctcgtagacc cgatcctgcg ggagggtctc gagcccggcg
     3781 ccctgcaggt agatgccgcg ctcgccgggc gtgaacgcgg ccgtgatcga tccgctcagc
     3841 gcgggatcct gtggttcgag ctggagcacc gtcgcctgct gggacacctc cgcgccgtcg
     3901 aacacggccg cgccgacgat cgcgccgccg acgaacagca ccagcgccgc cgccacggcg
     3961 acgagcggcc gcagccggct cccggccttt cgatgcgacc ctggaggctc gccgatcgcg
     4021 agctcgatcg tctcgtcggc gaagcccggg cgcagggcga ccggttcgag ggcgaaggcc
     4081 agccggcccc cgacctcttc gtactcggtc tcgaggcgcc ggcactcctc gcagtcgggg
     4141 gggtgcgagg ccatctcgcg ctcgagctcg gcctgctcct gcggatcgag cccgtagatc
     4201 gaacggaacg cgatgagctc ctcgatcctc tcgtggctgc gcatcatcgc ggccaccccc
     4261 cgatcgagcc ccggagccgg cgcatcccga gcagcgtccg cgacttcacg gtcccgagcg
     4321 gcagcccgag ccggtcggag atcttggcag cgtccagttg agtgttgctg agtgggcgtc
     4381 accggcggag aagaaaccgg agccagtcgg tcgctttggt gtcgacgcca gcgagctgca
     4441 gcggtgactt gccgttgtcg ttccgacggc aggagtcggt gaaccgcttg aaccggtagc
     4501 agccgacgag gagccgggag aattgctccg ccgactccat cgaggcgaag ccctccatga
     4561 gacggagctt cttccccagc tgcttgatga cgttctcggt gatgttcgcg tcggcgggca
     4621 tgcccttgac ccggtggtgc gtcatgtaga gctcgaagct ccgctccatc gcgccgatcg
     4681 tgtcgctgcg accaaggccc tggtagcgat cccgatccgc aagcaggtcg ccgaacagcc
     4741 ggcgcgcctc ctcctcggtg gtggcgaaca gcacggcacg gacccgatcc ttgagctctg
     4801 ctcgcagcgg ggcgtcgggc gagcgcttgg ccttggggat cgtaaagtcg agccggcggg
     4861 aggcgtggat ccggcaaagc tgcagcggag ctcgggcgag gtagttggcg tgggcagcga
     4921 ggaagggtgg tgaggcgtcg ctgaccaggc ccctcagtgg atagccgccc ttggagaccg
     4981 cctcccgcac caagcgctcg aaggcctcct ctccctcgat gtcggcaacc accgcatgca
     5041 ccacgtcgtg ggtgccctgg tccacgccga ccaacaacgc cgcctcatcc cccgcgacga
     5101 agatcgcctt gccgtccacg cccaggaacc cgctccagga ggtgggtccg agctcggcgg
     5161 agacctccac cggggtcttg gctcgtgcgc cgagctcgtc cacccacctg ttcagcgtga
     5221 accgcccgac ccgacgaccg aggcgctgtt ccaggagtac ggacaagacg cggtaggaag
     5281 gcagaccttg cacgtagagc cgcacggcct cctcgatggc gtcgtcggca aagcgcgccc
     5341 tcggccgcgc cgtccttcgt gcgggcgtga aggtccgccc gcagccgcgg cagaggaacc
     5401 gctggaggga ccccagagaa cccgccagtc cggtgggagc gctggaggtc ctaccgtgcc
     5461 tcttggtatc caggctcccg cacctcgggc agcggacgcg ccgttcgtct tgccatcccc
     5521 ggcctcccta tccacccgtt tgctggatag ctaccacgaa cagccaccct gacctgggat
     5581 ttcagtgccc cggcggacac actcaactgg acgctctcgc aatcttcgac tgcgagaggc
     5641 cgtcgaagta catcagctcg atgacctggc gttgttcggc ggggagctcg tcgagcgcgg
     5701 ctcgaaccgc ccggcgttcc tcgggcagcc cgatctcgtc gacgatatcc tcggccgggt
     5761 ccggctggac gaccgggtcc gagagctggg actcctcgac gcggcgccgc tgcgactcct
     5821 cgcggcgcac ggtgtcgacg gcgcggtgat ggaccatgcc catcagccat gcacggacgg
     5881 acccgcgctg cgggtcgtag ccgcccgggt tccgccacac cgccaagaat gcctcctgca
     5941 cgatctcctc cgcgaggaac ggctgccgga cgacgcggcg cgcgagtgcc atggcgctcg
     6001 gcgcgtaccg tccgaacaga cctcggaagg cttcgccgtc gccacggcgg atccgctgga
     6061 cgagctcccg gtctcgggcg tcggagaggt cggccgtcac ggaaacttcc cgtgacgcgc
     6121 gggcatcgag cgcctcgagg agcgaagagg gagccaccgt t
//
LOCUS       DASUYD010000027         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1777076, whole genome shotgun sequence.
ACCESSION   DASUYD010000027 DASUYD010000000
VERSION     DASUYD010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1777076"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..123
                     /locus_tag="VF195_01360"
     tRNA            48..123
                     /locus_tag="VF195_01360"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84..86,aa:Asp,seq:gtc)
     gene            200..272
                     /locus_tag="VF195_01365"
     tRNA            200..272
                     /locus_tag="VF195_01365"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:233..235,aa:Phe,seq:gaa)
     gene            complement(457..1698)
                     /locus_tag="VF195_01370"
     CDS             complement(457..1698)
                     /locus_tag="VF195_01370"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HEX6579513.1"
                     /translation="MAVAIVVLTAVLVLPTSGSVAAQNLPDQVLAWNQHAYDELLVTG
                     AQPPPLAILNMAAVHGAMYDAVNAIDRGHEPYLGSPASAKSWYSKNAAAAAAAYRVLL
                     DLLPAREPQLTGYYQASLATIPDGPRQEGGIDVGEAAAAAMIAFRANDGRTGNPLFTV
                     GTLAGEWRPLAVGGNNFQWVGEVRPFLIPGPEMFRTKGPLDLDSAGYAAEFNRVKALG
                     RATGSTRTQNQTDAALFWNENAVAMWTRIARQLSAGRGLNTVENARYFAMLYTTTADA
                     VISCFDDKERWHFWRPTTAIQLAGDDGNPDTVADPEWTSLLPVPPYPDHPSGHNCLSG
                     SVVRTLQQFYGTDQMSFSATNVIAEPDIARSFSSFSQAIHEIRRARIWGGLHFWTPDA
                     QGARLGRRVANFRQEHYFQPK"
     gene            complement(2161..2850)
                     /locus_tag="VF195_01375"
     CDS             complement(2161..2850)
                     /locus_tag="VF195_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579514.1"
                     /translation="MSDERLLLEWVGERFTFPEDAFGRLSRRRNRKVRNQRIASGLLA
                     VIVSLLAIAGLLRALTARETGDRTIDPKPGPSGAIPYRDNGLSRIARGRDLGYSWTLS
                     DQHADPRTDPGPYLVIEGPMAIPILVDPVNIWQIGCLDRGAYLTAATLPTVDRVWMRL
                     DEGTEFDARWMPIRNRQGRPLRLWLAFLPGSGRGVVRIGDRGGVPARWPIEPEAPTRE
                     ARFSCASAPPP"
     gene            complement(2847..3326)
                     /locus_tag="VF195_01380"
     CDS             complement(2847..3326)
                     /locus_tag="VF195_01380"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579515.1"
                     /translation="MAESSSFEAFFHDEARTLFRRLCAVTGNSSEAEEIMQDAFLALW
                     ERWDRISGMDDPTGYLYRTAMNVFRKRTRRAALAIRRTIASAPDPTPFSEIDAQQDLV
                     TAMAKLSPRQRAALVLTEVLDYSSEEAGRMLGVTAGTVRGLASRAREALRTQGEDRE"
     gene            complement(3430..4233)
                     /locus_tag="VF195_01385"
     CDS             complement(3430..4233)
                     /locus_tag="VF195_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579516.1"
                     /translation="MVPSAYLRVFQPLDAFEGEEQLRWERYLVSNRWVPGSPRRYRDH
                     AKGGLGLIAPADGEHAEIRVVDGRTYVSPWRMRMRVLAAGLAFRETRPIELSERFLSK
                     KDAKRVARELRRLRRRDPLAVAFCHQSPWHVPIRWFALFADEERWLGEDEHGQLRLRY
                     RTTARKALRRAGQTIPLLRRSDLGPIGDLLMDLHEWIALFDARSLVELDYGGLCDFLT
                     WDELDDDRSVRDLGLALEALTRHEFPRSAEIYQGVLAHWAEIRGHELLN"
     gene            4327..5241
                     /locus_tag="VF195_01390"
     CDS             4327..5241
                     /locus_tag="VF195_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEX6579517.1"
                     /translation="MKIVSLLPSATEIVFALGLGDSLVGVTDECDFPPEAVTKPVVSR
                     SALPQGRPLSAREIDQAVRGRLDAEEPLYVLDTDLLRREQPDVVLTQDLCRVCAVPSG
                     QVHQALEKLGLPSAKVLSLDPNTLEDVIAQIDVVGKLLERPEEAAKLTDSLRMRAAKV
                     KEVATRLPMLNVFCLEWSDPPFAAGHWVPEMVEAVAGVNLLSEKGGPSRVVTHREIRD
                     ANPEAIVFMPCGYYLEEAEEEAAAVFAHPELRETPAVRNGNVFAVDATSYFSRPGPRI
                     VDGLEILAWALHSDAYPVPPAGSISRVS"
     gene            5277..5480
                     /locus_tag="VF195_01395"
     CDS             5277..5480
                     /locus_tag="VF195_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579518.1"
                     /translation="MATNLVTPNDLGKRVSFQFELPNGYVGEAVGVLERWDEDAETYF
                     VRKKDGTEVRVPARGIRYGKIVE"
BASE COUNT          890 a         1940 c         1902 g          905 t
ORIGIN      
        1 ggtagcacgg gttcgaatcc cgttgggggc acggcaccac cacatatgcg cccgtagagg
       61 agtccggtcg tcctcacctc cctgtcaagg aggagatcgc gggatcgaag cccgtcgggc
      121 gcgctgggtg agcgagtcgg aaacggctcg ctcaccggtc tggggctcaa ggaacgagcg
      181 cggccctcag gcgggatccg gggaggtagc tcagtcggta gagcacgcgg ctgaaaaccg
      241 cggtgtcggc ggttcgatcc cgcccctccc caccgacgag cgggcgtcgg gcggcaacgg
      301 caacgcgctc gatggcgggg cggataccga cacgatcgac ggcggcgccg gaatcgaccg
      361 ggcgatcaac ggcgagaacg tgaccaacgt ccgctgagcg acgggtcggg aaggatgccc
      421 cctgttcggg ggcatcctcc cgcctctacg gtgcgcctac ttcggctgga agtagtgctc
      481 ctgccggaag ttcgcgaccc ggcgccccaa ccgggcccct tgcgcatccg gcgtccagaa
      541 gtgcagacca ccccagatcc tggcccggcg gatctcgtgg atcgcctggg agaagctgga
      601 gaagctgcgg gcgatgtccg gctcggcgat cacgttcgtc gcgctgaagg acatctggtc
      661 cgtcccgtag aactgttgca gcgttcggac gaccgaaccg ctgagacagt tgtgccccga
      721 cgggtggtcg gggtacggcg ggacgggcaa gagcgacgtc cactcgggat ccgcgaccgt
      781 gtcggggttg ccgtcgtccc ccgcgagctg tatcgccgtc gtgggccgcc agaagtgcca
      841 gcgctccttg tcgtcgaagc acgagatcac cgcatcggcc gtggtcgtgt acagcatcgc
      901 gaagtaccgg gcgttctcga ccgtgttcag accccgtccc gccgacagct gtcgagcgat
      961 ccgcgtccac atcgcaacgg cgttctcgtt ccagaacaac gccgcgtcgg tctggttctg
     1021 ggtcctcgtc gatcccgtcg ctcgcccgag agccttcacg cggttgaact cagccgcgta
     1081 tccggcacta tccaagtcca gcggtccctt cgtccggaac atctccggcc ccgggatgag
     1141 gaacggacgg acctcaccca cccactggaa gttgttcccg cccaccgcga gcggccgcca
     1201 ctcgccggca agcgttccca cggtgaacag cgggttgccc gtccgcccgt cgttggcccg
     1261 gaacgcaatc atcgcggcgg ctgcagcctc cccgacgtcg atcccgcctt cctgcctggg
     1321 gccgtccggg atcgtcgcga gcgaggcctg gtagtaccca gtcagctgtg gctcccgagc
     1381 ggggagcaga tcgagcagga cgcggtacgc ggcggccgcc gctgcggcgt tcttcgagta
     1441 ccaggatttc gcggatgccg gcgatcccag gtacggctcg tggccgcggt cgatcgcatt
     1501 gacggcgtcg tacatcgcgc cgtgcaccgc cgccatattc aggatggcga gcggcggcgg
     1561 ctgggcgccc gttacgagca actcgtcgta cgcgtgctgg ttccaggcga gaacctggtc
     1621 cgggaggttc tgtgctgcga ccgacccgga cgtcggcagc accagtacag ccgtgagcac
     1681 gacgatggcg acggccatcg cggctcccct tcgtttcctc aacatgatgc gtctcctccc
     1741 gggggtggga accgatagaa gctctccttc acgctgagcc gtcacctcct ctccgatcgt
     1801 tcggtatccg agcaccttgc gaggcgcgtg cttcgacggg caaggaccta cgacaccgtg
     1861 cgagcgttgc ggatgtcacc atgaggcccg tgcggtccat ctccgtcgtc actccagtcg
     1921 tctgtcactc tccgcaagga gccgaacgtc gtccatctgg cgatccgctg atgccatgga
     1981 tgcccaacga cgccacaacg cgaaggagcc tcgggcgatc atccgcatcc gcgcgtgctc
     2041 gcgagtcttg cttccatatg catgaaccct cgagggacgc ggaacgctgc tctcgatccg
     2101 ggatcgtcgc gtcgacctga gttctgagca tccggaggag gggcgatgaa gcaagaggca
     2161 ctacgggggc ggagcactcg cgcagctgaa ccgcgcctcg cgggtggggg cctccggttc
     2221 gatcggccac ctcgcgggga cacctcctcg gtccccgatc cgaacgacgc ctctgcccga
     2281 cccaggaagg aacgcgagcc acagccggag aggccgtccc tgtcggtttc ggatcggcat
     2341 ccagcgcgcg tcgaactcgg tgccctcgtc gagtcgcatc cagacgcggt cgacggtcgg
     2401 aagcgtggcg gcggtgagat aggcgccccg atccagacac ccgatctgcc agatgttcac
     2461 cgggtcgacc aagatcggga tggccatcgg tccctcgatc acgaggtagg ggcccgggtc
     2521 cgttcgcggg tcggcgtgct ggtcggagag cgtccaggag tagcccagat cccgcccgcg
     2581 cgcgatcctc gagagaccgt tgtcccggta gggtatcgct cctgacggac ccggcttggg
     2641 gtcgatcgtc cggtcgcccg tctcccgagc cgtgagggcg cgcaggagtc ccgcgatcgc
     2701 gagaagggac acgatcaccg cgagcaaccc ggaagcgatc cgctggttcc gaaccttccg
     2761 gttccgtcgc cgcgacagcc ggccgaacgc gtcctccggg aaggtgaatc gttcgcccac
     2821 ccattccagg agaagccgct cgtcgctcat tctcggtcct ccccctgcgt ccgaagagct
     2881 tcccgagctc gggacgcgag cccccgaacg gtccccgccg tgaccccgag catgcgaccg
     2941 gcctcctcgc tcgaatagtc gagtacctct gtcagaacca aggctgcccg ttgtcgaggg
     3001 ctcagcttcg ccatggcggt cacgagatcc tgctgcgcgt cgatctcgga gaacggcgtg
     3061 ggatctggag ctgatgcgat ggtccgtcgg atcgcgaggg cagcacgacg tgttcgcttc
     3121 cggaagacgt tcatcgcggt gcgatagagg tacccggtgg gatcatccat cccggagatg
     3181 cgatcccacc gttcccagag ggcgaggaac gcgtcctgca tgatctcctc tgcctctgac
     3241 gagttccccg tcacggcgca gagacgtcgg aagagggtcc gcgcctcatc gtggaagaag
     3301 gcctcgaagc tcgatgactc ggccacggct ggcgtctccc cgtccgaaga tccagtcacg
     3361 agtcggagcg gttcgtccat cccgcagatg aacccccggc gggcgatcag cgttgtcggt
     3421 gggtcgtgct cagttcagga gctcgtggcc tcggatctct gcccaatgcg ccagcacacc
     3481 ctggtagatc tcggccgacc gtgggaactc gtgccgcgtc agcgcctcca gcgcgaggcc
     3541 cagatcgcgc acgctgcgat cgtcgtcgag ctcgtcccac gtgaggaagt cgcagagtcc
     3601 cccgtagtcg agctccacga gcgaccgggc gtcgaacagc gcgatccact cgtggaggtc
     3661 catcaggagg tcgccgatcg gccccaggtc ggaccttcgc aggagcggga tcgtctggcc
     3721 cgctcgacgg agtgccttgc gggcggtggt tcgataccgg agccgcagtt gcccgtgctc
     3781 gtcttccccc agccaccgct cctcgtccgc gaagagcgcg aaccaacgga tcggcacgtg
     3841 ccacgggctc tggtgacaga aggccaccgc gagcgggtcg cgccgacgga gccgcctcag
     3901 ctcgcgggcc acgcgcttcg cgtccttctt cgagaggaag cgctccgaga gctcgatcgg
     3961 acgggtctcg cggaacgcca ggcccgcggc cagcacgcgc atccgcatcc gccaggggct
     4021 cacgtaggtc cgaccgtcca cgacccggat ctccgcatgc tcaccgtccg cgggcgcgat
     4081 cagccccagc cctcccttcg cgtggtcgcg gtagcgccgc gggctcccgg gcacccaccg
     4141 gttggacacg aggtaccgct cccaccgcag ctgctcctct ccctcgaacg cgtcgagggg
     4201 ctggaacacg cgcaggtagg cgcttgggac catctccggg agtctagcga cggagggcat
     4261 ctcctatgct ctcggcgtcc tcgacccagg agctgaagtg ggatcgcccc ggtaccgtag
     4321 cgcgccatga agatcgtctc cctgctcccg tcggcaaccg agatcgtgtt cgctcttggc
     4381 ctcggagact ccctcgtcgg cgtcaccgac gaatgcgact tcccgccgga ggcggtgacg
     4441 aagccggtgg tgtccaggag cgcgctcccc cagggacgtc cgctgtccgc ccgcgagatc
     4501 gatcaggcgg tgcgcggcag gctggacgcg gaggagcccc tctacgtcct ggacaccgac
     4561 ctcctccggc gcgagcagcc cgacgtggtc ctcacccagg acctgtgccg ggtctgcgcc
     4621 gtcccctccg gacaggtcca ccaggcgctg gagaagctgg gcctccccag cgccaaggtg
     4681 ctctctctgg acccgaacac cctcgaagac gtgatcgcgc agatcgatgt ggtcgggaag
     4741 ctcctcgagc gaccggagga ggctgccaag ctcaccgact ccctccggat gcgggctgcg
     4801 aaggtgaagg aggtcgccac gcgccttccg atgctgaacg tgttctgcct ggagtggtcc
     4861 gacccgccct tcgccgcagg tcattgggtg ccggagatgg tggaggccgt ggccggcgtg
     4921 aacctcctct cggagaaggg cggtccgtcg cgggtcgtga cgcatcgtga gatccgagat
     4981 gcgaatcccg aggcgatcgt gttcatgccg tgcggctact acctcgagga ggccgaggag
     5041 gaggccgccg ccgtgttcgc gcaccccgaa ttgcgggaga cgcccgccgt ccgcaacggc
     5101 aacgtcttcg ccgtggacgc gacgtcgtac ttctcgcgac ccgggcctcg gatcgtcgac
     5161 gggctcgaga tcctggcgtg ggcgctgcac tcggacgcct accccgtccc gccggcgggc
     5221 agcatctcgc gggtgagctg agccggctgt tcggcgcgac gcgctatcct cgcgcgatgg
     5281 ctacgaacct cgtcacgccc aacgacctcg gtaagcgcgt gtccttccag ttcgagctcc
     5341 cgaacggcta cgtcggcgag gccgtcggcg tgctggagcg atgggacgag gacgccgaga
     5401 cctacttcgt gcggaagaaa gacgggacgg aggtgcgggt gccggcacgg ggcatccggt
     5461 acggcaagat cgtggagtga gccggcgggc tacgacgcct tggcgagtcc gccgaggacg
     5521 ccgatgagct cctcgacgtc gaacggcttc gggacgaacg cgtcggcgcc gagggcggtg
     5581 gccttcgcct tgtcgtccgg accggtgagc gcgctgacga tcacgatccg cgggcgc
//
LOCUS       DASUYD010000028         7788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1116279, whole genome shotgun sequence.
ACCESSION   DASUYD010000028 DASUYD010000000
VERSION     DASUYD010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7788
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1116279"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..212)
                     /locus_tag="VF195_01400"
     CDS             complement(<1..212)
                     /locus_tag="VF195_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6579519.1"
                     /translation="MATLREPATLFEAALGRLGDVLTPGSLLEATPESLVVAESGGEI
                     VYANTACERLTGFARGELLGRTVHGLL"
     gene            complement(220..1665)
                     /locus_tag="VF195_01405"
     CDS             complement(220..1665)
                     /locus_tag="VF195_01405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015717862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6579520.1"
                     /translation="MSVFEAVLAVLGVVTIVYFAVLSLLYGAFALIGLRAVILESRQT
                     SDTELRDLMERDVFKPVSIIVPAFNEERSIVAAVRSFVTLHYPKFEVIVISDGSTDGT
                     MDRLIEAFALVEEPRVWARTLSTEPVTRVMRSLRDPGLIVAEKANGGKADAVNAGINL
                     ARYPLVAPVDSDCLLDAQAILRATRNFVKDDSVIAVGGTVRPLNGATVVGGRATALQM
                     PRRWVERLQIVEYARAFFLGRAGWSRVGALLIISGAFGIFRRDAVLKVGGFWTETLGE
                     DMELVMRLHKTFARSGLPYRIVFSPDPICWTEVPSDLPTLLRQRSRWHRGLLTNLWRH
                     RDMLCNPRFGRLGLFAVPFFWLFEGLGPVVEVIGFTSLLVSAALGILDMHVFWLFLGL
                     AVLHGMVLSQVAAGVEAMLLQRYPSTSDRLILFAASLVEFLGFHQLVVIERFLATFQT
                     GARRRQWGVMARTGIPSEPAAPSQVATSNRR"
     gene            complement(1662..2870)
                     /locus_tag="VF195_01410"
     CDS             complement(1662..2870)
                     /locus_tag="VF195_01410"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6579521.1"
                     /translation="MWRRYGDVTFAVIFSLAISLEAIPLGYLMWRLASGIFGSSDGAR
                     LGSVLLGAVGSTAAALLLVTTYVLAYQHVSARHELVRAQRRQEWVGRWLGVLSGATTE
                     PAGSLEIEAVEALLALRETLRGSESTRVAEMLERHGAVVELERRTGHGSVSGRLDAIV
                     ALSNARIPSALMTLVNGVYDSNQLISVASARAAARTLARVDDLFDRDRGAAALARAME
                     TQELPYGIVEEVVVLSEESATALIAVLLQHERPRAPTVRAAIEGIGRLKLLIFAEEVV
                     PFLTHADDEVRGAALRAVSRLGFLPEASHGVVIAALADEVDFIRIHAATAARLLPHTQ
                     ALSLLSDRLGDRSWWVRRAAADALVGLGPAGLAQLGRAAREHADRFARDMAEQALRDQ
                     ISNLVRAVVA"
     gene            complement(2874..3248)
                     /locus_tag="VF195_01415"
     CDS             complement(2874..3248)
                     /locus_tag="VF195_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016329925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579522.1"
                     /translation="MTGILAIEDDWTVRAVLEHTLTSAGYDIDLVPGISDARQLLAEG
                     HYDLVLLDLNLPDGNGLDLLRDLREDLGTSVPVLVLSGLRQEEVVVRGLELGADDYVT
                     KPFSPPELLARVSRCLGQRTGR"
     gene            complement(3331..4548)
                     /locus_tag="VF195_01420"
     CDS             complement(3331..4548)
                     /locus_tag="VF195_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019057045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX6579523.1"
                     /translation="MRILVVGSGGVGSAFAPIVARRDFAEHVVFADHDEGRARSIVDR
                     YGDGRFSAARVDASEAEAVAELATANRCDAILNAVDPRFVMPVFEGALAAGATYLDMA
                     MSLSEPHPERPHEETGTKLGDEQFARADAWEERGLLALVGIGVEPGLSDVFARHAADE
                     LFDTIDEVGVRDGANLVVEGYDFAPSFSIWTTIEECLNPPVVWEKDRGWFTTEPFSEP
                     EVFTFPEGIGPVECVNVEHEEVLLVPRWIECRRVTFKYGLGDEFIEVLKTLRKLGLDR
                     TEPVSVNGLNVSPRDVVAAALPDPMSLGDRMAGKTCAGTWVTGTGADGSPREVYLYHV
                     VDNEETWRRDGAQAVVWQTALNPAIALELLANGAWTGTGVLGPEAFPPGPFLDLLHEH
                     GSPWGLEERSPRS"
     gene            complement(4710..6824)
                     /locus_tag="VF195_01425"
     CDS             complement(4710..6824)
                     /locus_tag="VF195_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX6579524.1"
                     /translation="MNRSTVRAGSFRRSVAALGVMSLIAFSAVLILAGTAVAAPFTGG
                     LSPTVIDGLLDLNGDNEVTGADDSNAFYGDTAIINGGLDCDAWSGVNGGEAGDGVIDA
                     NDDCTMLAYDGTVDGVTITVTDGTVVWPDGQFPTVFPDPSDPDNPGVLESNFAWSTIN
                     GLVDSSGDETIDANDCSFDVVGTADILGNTVANTNPCGFANPPSAADNGKVDLNSDGD
                     ITAADTCEDGCFLGHNVTLGVVQAEGATVTTPSNAFSGTFGPTIIGGGADLNGDGVVD
                     GRDDSNAFFGDTSIIDGQLDCNNWSTDNDGTAGDLAITIADDCTLIGFDGTPNGVTVN
                     VIDGEFGWTGPLPTVFNATDPDNPDVGDSDFAWSAIGGRVDSSGNEIITSEDCHFGLI
                     GATVDAGLGDPTDGADILANDLAMTNPCGFGVVAGPDPDFNGLVDLNSDLTITAADSC
                     DDCFFGLDLDNGFVVGSGAGTAETLDLAPSVGTNSEDTPHTVTATVEDSNLDPVEGVT
                     VEFTVTGVNSATGDAVTDASGVAAFTYTGATVGVDTITAFADTDGDGINDVAEPEDTA
                     TKTWTPTEPTCPGFAGDPRNQVVGTTGADVLAGTPGADIICGLGGSDTLNGLGGNDIL
                     FGGGGSDVLRGGLGNDRLVGGSGQDRLVGASGRDRLFGGIGRDRLFGGPGRDLLHGGP
                     GRDFGAGGPGRDTFRSIEIRRP"
     gene            complement(7255..>7788)
                     /locus_tag="VF195_01430"
     CDS             complement(7255..>7788)
                     /locus_tag="VF195_01430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6579525.1"
                     /translation="PGVDVRRSSAEDIPHPDGAFDVAMAQLVVHFMRDPIAGLTEMAR
                     VTRPDGLVAACVWDFTGERAPISAFWQAAREADAEVHDESDLAGAREGHLTELFEEAG
                     LRNVEETALLVSVEHETFDDWWEPFELGVGPAGAHLDQLSPQERAALRERCRSILPPL
                     PFTMTTAVWAARGSTTT"
BASE COUNT         1268 a         2719 c         2611 g         1190 t
ORIGIN      
        1 agcagcccat gcaccgtgcg tccgagcagc tcgccgcgcg cgaagcccgt caggcgctcg
       61 cacgccgtgt tcgcgtacac gatctcaccg ccgctctcgg ccacgaccag ggactcgggg
      121 gtcgcctcca ggagagagcc gggcgtgagc acgtcgccga ggcgcccgag ggcagcctcg
      181 aagagcgtgg cgggctctct gagcgtggcc atgctcaggt catcggcggt tcgatgtggc
      241 gacctgagac ggcgccgccg gctccgaggg gatcccggtc cgggccatga ctccccactg
      301 ccgcctcctc gccccggtct ggaaggtggc gaggaatcgc tcgatcacga cgagctggtg
      361 gaaccccagg aactccacga gcgaggcggc gaagaggatc agccggtccg acgtggatgg
      421 gtaccgctgc aggagcatcg cctcgacccc tgccgccacc tgcgacagga ccatcccgtg
      481 gaggacggcg agtccgagga agagccagaa gacgtgcatg tcgaggatgc cgagggcggc
      541 cgacacgagc agcgaggtga acccgatcac ctcgaccacg gggccgagcc cttcgaacag
      601 ccagaagaat gggacggcga acaggccgag cctgccgaag cgcgggttgc agagcatgtc
      661 cctgtgccgc cacaggttgg tcagcaggcc gcggtgccaa cgactccgct gccgcaggag
      721 ggtcggcaga tccgacggca cctcggtcca gcagatgggg tccgggctga acacgatccg
      781 gtacggcagg cccgatcgag cgaacgtctt gtggagccgc atgaccagct ccatgtcctc
      841 accgagcgtc tccgtccaga acccgccgac cttgaggacc gcgtcccgac ggaagatgcc
      901 gaacgcgccg gagatgatca acagcgcacc cacccggctc caaccggccc tgccgaggaa
      961 gaacgcccgc gcgtactcga cgatctggag ccgttccacc cagcgccggg gcatctgcag
     1021 cgcggtcgcg cggccaccta cgactgttgc gccgttgagc ggcctgaccg tcccacccac
     1081 ggcgatcacg ctgtcgtcct tcacgaagtt gcgggtggcg cggaggatcg cttgtgcgtc
     1141 gaggaggcag tcggagtcga ccggggcgac gagcggatac cgcgccagat tgatgccggc
     1201 gttcaccgcg tcggctttcc ccccgttcgc cttctcggcg acgatcaggc ccgggtcgcg
     1261 gagcgagcgc atcacccggg tcacgggctc cgtcgacagc gtccgcgccc agacgcgagg
     1321 ctcctcgacg agggcgaacg cttcgatcag ccgatccatc gtgccgtccg tcgacccgtc
     1381 ggagatcacg atgacctcga acttcgggta atggagcgtg acgaaggatc gcaccgcggc
     1441 gacgatgctc cgctcctcgt tgaaggccgg aacgatgatc gagaccggct tgaagacatc
     1501 tcgctccatg agatcgcgga gctccgtgtc cgaggtctgc ctcgattcga ggatgaccgc
     1561 acggagtccg atcagcgcga aggcgccgta gaggaggctc aggaccgcga agtacacgat
     1621 cgtcacgacc ccgagaacgg cgagcacggc ctcgaacacg ctcatgcgac caccgcccgc
     1681 acgaggttgg agatctggtc gcgcaacgct tgctcggcca tgtccctggc gaagcgatcg
     1741 gcgtgctccc gagcggcccg tcccagctgg gcgagcccgg cgggaccgag acccaccagg
     1801 gcgtcggcgg ccgcccggcg gacccaccac gaccgatccc cgagtcgatc gctgaggagg
     1861 gacagcgcct gcgtgtgtgg gaggaggcgc gctgcggtgg cggcgtggat ccggatgaag
     1921 tcgacctcgt cggcgagcgc ggcgatcacg acgccgtggg acgcctcggg caggaacccg
     1981 agccgactta ccgctcgcag cgccgcgccg cggacctcgt cgtccgcgtg ggtgaggaac
     2041 ggcacgacct cttcggcgaa gatcaggagc ttcagccgcc cgatgccctc gatcgcggcc
     2101 cggaccgtgg gcgcgcgagg tcgctcgtgt tgcagcagga cggcgatgag cgcggtcgca
     2161 gactcctccg acaggaccac cacctcctcg acgatcccgt agggcagctc ctgggtctcc
     2221 atcgctcgcg cgagggcggc cgctccgcga tcccggtcga agagatcgtc cacccgagcc
     2281 agcgtccgtg cggcggctcg tgcggatgcg acgctgatca gctgattcga gtcgtacacg
     2341 ccgttcacga gcgtcatcag cgcggagggg atacgggcgt tcgagagcgc gacgatcgca
     2401 tccaggcgac cgctcacgga accgtgcccg gttctgcgct cgagttccac gaccgctccg
     2461 tgacgctcga gcatctcggc gacgcgcgtc gattcggaac ctcgcagcgt ctcccgaagc
     2521 gcgaggaggg cttcgacggc ctcgatctcg aggctgcctg ccggctccgt ggttgctccg
     2581 ctcaacacgc cgagccatcg gccgacccac tcctggcgtc gttgcgcacg gacgagctcg
     2641 tggcgggcgc tcacgtgctg gtacgcgagg acgtaggtgg tgaccaacaa cagcgccgcc
     2701 gcggtcgatc cgaccgcgcc gaggaggacg ctccccagtc gggcaccgtc ggaggagccg
     2761 aagatccccg acgccaacct ccacatcagg tatccgagcg ggatcgcctc gagggagatc
     2821 gcgagggaga agatcaccgc gaaggtgacg tcaccgtacc gccgccacac gggctacctc
     2881 cctgtgcgtt gacccagaca gcggctcacg cgcgcgagca gctccggcgg gctgaacggc
     2941 ttggtcacgt agtcgtccgc cccgagctcc agcccccgca cgacgacctc ctcctgacgc
     3001 aggccggaga ggacgaggac gggcacgctg gtgccgaggt cctcccgcag gtcgcggagc
     3061 agatccagcc cgttgccgtc gggcaggttc aggtccagca acacgaggtc gtagtggccc
     3121 tcggcgagga gttgcctcgc atccgagatg ccgggcacca ggtcgatgtc gtagccggcc
     3181 gacgtcagcg tgtgctcgag cacggcccgc acggtccagt cgtcctcgat cgctaggatc
     3241 cccgtcatcg ctcctccagg cacgctgcag tcgacccgct ggtcgctgct tgtgacatcg
     3301 gtcggagagg gagggcgctg aaggaggggg ctagcttcgg ggcgagcgtt cctcgagacc
     3361 ccacggcgat ccgtgctcgt gcaagaggtc gaggaacgga cccggaggga acgcctccgg
     3421 gccgagcaca ccggtgcccg tccacgcgcc gttcgccagg agctccagcg cgatcgccgg
     3481 gttcagggcc gtctgccaga ccactgcctg cgcgccgtca cgtcgccagg tctcctcgtt
     3541 gtccaccacg tggtagaggt agacctctcg aggcgagccg tcggccccgg tgccggtgac
     3601 ccacgtgccc gcgcatgtct tcccggccat ccggtcgccg agggacatcg ggtcgggtag
     3661 cgcagccgcc acgacatcac gcggcgacac gttcagcccg ttgacgctga ccggctcggt
     3721 ccggtcgagg ccgagcttcc gcagcgtctt cagcacctcg atgaattcat cgcccagccc
     3781 gtacttgaac gtcacgcgcc ggcactcgat ccaccgcggc acgaggagca cctcctcgtg
     3841 ttccacgttc acgcactcga cgggaccgat cccctccggg aacgtaaaga cctcgggctc
     3901 gctgaacggc tcggtcgtga accacccgcg atccttctcc cacaccaccg gcgggttgag
     3961 gcattcctcg atcgtcgtcc agatcgagaa gctgggagcg aagtcgtacc cctcgacgac
     4021 gaggttcgcc ccgtcacgga ccccgacctc atcgatcgtg tcgaacagct cgtcggcggc
     4081 gtggcgtgcg aacacgtccg agagccccgg ctccaccccg atgcccacga gcgcgaggag
     4141 cccgcgctct tcccaggcgt cggcccgggc gaactgctcg tccccgagct tcgtgccggt
     4201 ctcctcgtgc gggcgctcgg gatggggctc cgagagtgac atcgccatgt cgagataggt
     4261 cgcgcccgcc gcgagggcgc cctcgaacac cggcatcacg aagcggggat ccacggcgtt
     4321 caggatcgcg tcacaccggt tggcggttgc gagctcggcg acggcctcgg cctcgctcgc
     4381 atcgacacgg gcggcggaga agcggccgtc tccgtaccgg tcgacgatgc tccgcgcgcg
     4441 accctcatcg tggtcggcga agacgacgtg ctcggcgaaa tcgcgccggg cgacgatcgg
     4501 cgcgaacgcc gaccccacgc cgccggaccc gacgaccagg atcctcatgg gcgcggagtg
     4561 tagccgtcga ccacgcgagt gctcagcgag accgaacggc ggcggcgagg agggagatca
     4621 cgtccggatc gcgttcgtcg tcgggcagcc cgggcatggg aggagccggc ccgagcgggc
     4681 cggctcccgt gtgtgccgat cgccgacggc tatggccgcc ggatctcgat actcctgaac
     4741 gtgtctcgac cgggccctcc ggctccgaag tcccgaccgg ggccgccgtg caggagatct
     4801 cttccgggtc cgccgaacag acggtccctg ccgatcccgc cgaacagacg gtccctgcca
     4861 gacgcgccga ccagccggtc ttgtccagac ccgccgacga gtcggtcgtt gccgaggccg
     4921 ccgcggagga cgtcggaccc accgccgccg aagaggatgt cgttcccccc gaggccgttg
     4981 agagtgtcgc tcccaccgag accgcagatg atgtcggccc cgggggttcc cgccaggacg
     5041 tcagcgcccg tggtcccgac cacctggttc cgcggatcgc ccgcgaagcc cggacaggtg
     5101 ggctctgtcg gtgtccacgt cttcgtggcc gtgtcctccg gctcggcgac gtcgttgatc
     5161 ccatcaccat cggtgtcggc gaacgccgtg atcgtgtcga cgccgacggt cgctccggtg
     5221 tacgtgaatg ccgcgacccc gctggcgtcg gtgaccgcat cacctgtcgc cgagttgacc
     5281 ccggtgacag tgaactccac cgtgacgccc tcgaccggat ccaggttcga gtcctccact
     5341 gtggccgtca ccgtatgtgg cgtgtcctcc gagttggtgc ccaccgaggg cgcgagatcc
     5401 agcgtctcgg ccgtccccgc accgcttccc accacgaacc cgttatcgag gtcgaggccg
     5461 aagaagcagt cgtcgcacga gtcggcggcc gtgatggtca agtcactgtt gaggtccacc
     5521 agcccgttga agtccggatc cggcccagcg acaacgccga acccgcaggg gttcgtcata
     5581 gccagatcgt tggcgaggat gtcggctccg tccgtcggat cgccgagccc ggcgtcgacg
     5641 gtcgcaccga tcagcccgaa gtggcagtcc tccgaggtga tgatctcgtt gccgctggag
     5701 tccacccttc cgccgatcgc cgaccacgcg aagtctgagt caccgacgtc ggggttgtcc
     5761 gggtcggtcg cgttgaacac ggtcgggagc ggtccggtcc acccgaactc gccgtcgatc
     5821 acgttgaccg tgaccccgtt cggcgtgccg tcgaatccga tcagcgtgca gtcgtcggcg
     5881 atcgtgatcg cgaggtcgcc ggcggtgcca tcgttgtcgg tgctccagtt gttgcagtcc
     5941 agctgaccgt cgatgatcga cgtgtcaccg aagaacgcgt tcgagtcgtc gcgcccgtcc
     6001 acgacgccgt cgccgttcag atccgcgcca ccgccgatga tggtcggtcc gaacgtgccg
     6061 ctgaatgcgt tcgagggagt ggtcaccgtg gcgccttctg cctgcacgac cccgagggtg
     6121 acgttgtggc cgaggaagca gccatcctcg cacgtgtccg cggcggttat gtcgccgtcg
     6181 ctgttcaggt cgaccttgcc gttgtcggca gcacttggag ggttcgcgaa cccacacggg
     6241 ttcgtgttcg cgacggtgtt cccaaggatg tccgccgtgc cgacaacatc gaaggagcag
     6301 tcgttcgcgt cgatcgtctc gtcaccactg gagtccacga ggccattgat cgtcgaccac
     6361 gcgaagttgg attcgagcac ccccgggttg tccggatcgc taggatccgg gaagacggtc
     6421 gggaactgtc catcgggcca gacgacggtc ccgtcggtga cggtgatagt gacgccgtcc
     6481 actgtgccgt cgtaggcgag catcgtgcag tcgtcgttgg cgtcgatcac accgtcaccg
     6541 gcttcgccac cgttcacacc cgaccaggcg tcgcagtcga gaccgccgtt gatgatcgcg
     6601 gtgtcgccgt agaaggcgtt cgagtcgtca gcgccggtga cctcgttgtc gccattcaga
     6661 tcgagcagtc cgtcgatcac ggttggcgac agaccaccag tgaatggcgc cgctaccgcc
     6721 gtacccgcca ggatgagcac tgcggagaac gcgatcagcg acatcactcc gagggccgcc
     6781 acggagcggc ggaacgatcc tgcacgcact gtcgatcggt tcatacgcat ggtgctcctt
     6841 ccgagtcggg cacggtccgc ggcgctcgca tgccgcggtc gagtgcgtag gtagcgaggg
     6901 gaggtatcgc agatcgccgg ctgagacata gtcgaacgcc gaacgtgacc agacccatac
     6961 acaccccccg gcgcttctct cgccacggga ttccacctcc gcggaaaccg acgcattcgc
     7021 tttcgcgaaa ccatttcgga ggctaatccc ttgtcctcga agaagcaaat ccgcgcttgc
     7081 gctgttcagg aggctccgag cgtctaccgg gagcccggtg gccgtttcgt gagaggggct
     7141 cccgctaagt ccggttgttc ctcccgagag aaccggcatg tggcggacgg aatcggctct
     7201 cggccaggca tctgaccgcg aggtcgaaga ccatccatgc ggtcgcactc tccctcacgt
     7261 cgtggtggac cctcgcgcag cccagacggc ggtggtcatc gtgaacggca gcggcgggag
     7321 gatcgaccta cagcgctcgc ggagcgcggc tcgttcctgc ggcgagagct gatcgagatg
     7381 cgcgccggcg ggcccgaccc ccagctcgaa cggctcccac cagtcgtcga aggtctcgtg
     7441 ctcgacggac acgaggagcg ccgtctcctc cacgttccgg agccctgcct cctcgaacag
     7501 ctctgtgaga tgtccctcac gcgcaccggc gaggtcggac tcgtcgtgga cctcggcgtc
     7561 cgcctcacgg gctgcctgcc agaacgcgga gatcggtgcg cgctcccccg tgaagtccca
     7621 gacgcaggcc gccacgaggc cgtccggccg cgtcacacgc gccatctccg tgagtccggc
     7681 gatcgggtcg cgcatgaaat gcaccacgag ctgcgccatc gccacgtcga atgcgccgtc
     7741 cggatggggg atgtcctcgg ccgaggatcg ccggacgtcg acgccggg
//
LOCUS       DASUYD010000029         8375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_676088, whole genome shotgun sequence.
ACCESSION   DASUYD010000029 DASUYD010000000
VERSION     DASUYD010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676088"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VF195_01435"
     CDS             complement(<1..740)
                     /locus_tag="VF195_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX6579526.1"
                     /translation="MAPFDPDPRQGETLEHTDGAMLVTGGSGTGKTAVLQERFARLVE
                     AGADPERIALIVGSRRARDEARRLLLERLPVALPTLTVVTIHGLAFHVVGERFEQLGY
                     GAPPTVLSASEQFARVRELLADQDLNRWPTYGPLLPLRGFADEVQQFVIRAQESLLSP
                     DDIEHRVAERGLAGWNELAAFLREYLSVLGNKEPAEIDFAGLVGQAANAARAGDPPFD
                     HVMIDDYQDTTFAAERFLAELRPRSFVAA"
     gene            complement(789..1886)
                     /locus_tag="VF195_01440"
     CDS             complement(789..1886)
                     /locus_tag="VF195_01440"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX6579527.1"
                     /translation="MFGRGFRIATIRGVPVNVDSSWIWIAVLLVATFWAQLDRDFPNL
                     ASAEIVGYALFGALVFFGSVFLHEAAHAVAARANGIEVHGITLVFFGGFTAARADEKG
                     PGPAFAIAALGPGTSLALAAVFWILSRLTESTTGPLPGLFSYVSAVNLFMAVFNALPG
                     LPLDGGRMLQSVVWRVTGDQRKATRVAARAGLVVGGIVVALAIFALARQQLFEAVWGG
                     IIGMFIFRGAQASEQRIGIDDRLASATVADAMDPPPPTVPADLSLSQTLDRFLRGHEE
                     EAFPVLEDGNVIGMISFSSARELGMRDPLRPARDAVIPLEHVLIAHPDERLDEVSVRL
                     GTERAALVLRDGQLVGAISGSGVYRWAARAS"
     gene            1950..3470
                     /locus_tag="VF195_01445"
     CDS             1950..3470
                     /locus_tag="VF195_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6579528.1"
                     /translation="MGDRVDRPTEPEPTHRVRKAATVLLACVVGLVGAWLGMALLARD
                     TVPVGPFRVELDVGFGRGETVLGLPPFGDLTADTHLSPLHVSATLRDVGVQRLTDVVN
                     DEGIDGLVSDVERDAQDRVRTMAWRVLVVGTLGGAAFAGLVYRRRWRLVTAGAIAALL
                     GVVLSEVLLLATFQPAAFAEPTYSGSLALAPQLIGPAREATDRVEDLRAGLEEIVDGA
                     VRAYTTLQATPLADDAIRVLHISDIHASPLGMDFAQEVARGFDVDFVIDTGDITSFGT
                     PVENLITSQIPGFGRPYVFVRGSHDSIALQEAVADQSNAIVLDGRARTIEGVTVYGLG
                     HPAFTPARGAPVDAEDFAALARSAGPVIAAGLEASAEPVDVVAVHDDRMAEAVAGRVP
                     LVISGHFHDTVASVEDGTLYLRIGTTGGSGAGIFRDLADIPFTAEVLYFSRGEDPELI
                     AYDVIEQFPESGSLTVQRVTVPVEFGELVLTPTPAPTPSVSPSGASPSASPDPNAT"
     gene            complement(3699..4598)
                     /locus_tag="VF195_01450"
     CDS             complement(3699..4598)
                     /locus_tag="VF195_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6579529.1"
                     /translation="MSKILVGTASWTDPTLVKDGHFYPPEAKSAEARLRFYATQFPIV
                     EVDSTYYFPPSEKNAVLWIDRTPNDFTFNIKAYSLLTNHPTKRESLYKDLLEELPDEL
                     DDKKNLYRENLPDAAVDEVWQRFHDALMPLHSSGKLGAVLFQFPQWFVIGRKNKDYIV
                     ECAERLKDFRLAIEFRHKTWLEERNAEETLSFLQEHDLPLVCVDMPQGFDSSIPPIAA
                     ATAKDLALVRFHGRDRKAWAKKNVSASERFRYEYSEQELEEWVPRIHDISDQSRETHV
                     LMNNCYRDFAVNNARQLADLLEE"
     gene            4829..6202
                     /locus_tag="VF195_01455"
     CDS             4829..6202
                     /locus_tag="VF195_01455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579530.1"
                     /translation="MLGTVLLDLAILTYGAITSSDGFRDAGWGLAAWVVVVAAVGAAS
                     LPFESGQALGLDMPVLLSVGYLFGPVVAGATAFVAYVDARELRGEIPLVRAVFNRAQT
                     SLSVMAATAVFGVVVSGGDGWPIAVVAAFLAVGTDCLVNYGTVVAVLCLHERITPWDG
                     LSRLYFGFFPEFALTYASFGLLSLMLAEIYKSVGAWSLAVFVIPVLLARQAFAGHKRL
                     ASAARRLDTQAEALRDVAASVADERREERLSLAAGLHDEVLPPLFKVHLMGQVLRQDL
                     ASGRLLALEEDLPALLGATENASDSMRELIRSLRNSPLGTGGLPDTLRLLVRYLERES
                     TARISLSCERIGGTPLIQLLAYQVAREAIRNALKHARASEINIHVVDVEGNLRITVED
                     DGIGFEPDSVDQQVHFGLALMRERIEIAGGVLYVDSRLGEGTRIIARLPSNHEQSKGG
                     PPKETRP"
     gene            6570..8087
                     /locus_tag="VF195_01460"
     CDS             6570..8087
                     /locus_tag="VF195_01460"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6579531.1"
                     /translation="MSSDHGSIKAAKSARLLPFFEAAVVIPTLGALTFAIARDGLAEE
                     SELPQLVGWILIIALVELIPVPVWRGVHIGLGFPLMMAVAFMYPPQAAAAVALLAASD
                     PREFRREVGLLRALFNRCQVALAVGSASLIFYELADLDTSSPVELIAVAGLASIADYV
                     VNTTLVCAAASIHFWMNPLRVIRELKVGRLSEYLVSYLGLSVFGLMLAKFLSGPGNNW
                     WAVPVFLLPLLLARQMFFRSKALEEAHKELQDREHVLRALSNRMAEERQDERMQIAAF
                     LHDDLAQLLFRLSIQVDVARRHLDAGKVDQTEELLLEIKETKNRTSDRIRALIRDLHR
                     SPLGRAGLGEAIRGFLVEMARESDVRLHADIDEVDVPAPIALLLYHNAREGVMNALKH
                     AKASNIWVAVEQEGDEVEMVLRDDGVGFDVEAPGPEGHYGMTMMRERATVGGGTFAIK
                     SVPGEGTTITVRFPTSWLQEEESQNQAQQQAPALPPAGASRGAAPPASPTESIPA"
     gene            8171..>8375
                     /locus_tag="VF195_01465"
     CDS             8171..>8375
                     /locus_tag="VF195_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579532.1"
                     /translation="MTSARKVSRSSSVSRWCFWAWPSSWRYASTSSWPESIRASALAG
                     IPPFTAITAKLQARPITTRNIPGI"
BASE COUNT         1385 a         2653 c         2821 g         1516 t
ORIGIN      
        1 gcggcgacga agctccgagg ccggagctcg gcgaggaagc gttcggccgc gaaggtcgtg
       61 tcctggtagt cgtcgatcat cacgtggtcg aacggtggat ctccggcacg ggccgcgttc
      121 gcggcctgcc ccaccagtcc ggcgaagtcg atctcggccg gctccttgtt tcccagcacg
      181 gagaggtatt cacggaggaa cgcggcgagc tcgttccaac cggcaaggcc tcgttcggca
      241 acccggtgct cgatgtcgtc aggagagagg agggactcct gggctcggat cacgaattgc
      301 tggacctcgt cggcgaatcc gcgcaacggc aggaggggtc cgtacgtcgg ccatcggttg
      361 agatcttgat cggcgagcag ctcacgtacc cgagcgaact gctcggacgc cgaaaggacc
      421 gtggggggcg cgccgtatcc gagctgctcg aagcgctcgc cgacgacgtg gaacgcgagg
      481 ccgtggatcg tgacgacggt cagcgtcggc agggccaccg ggaggcgttc gagcaggagg
      541 cggcgggcct cgtccctcgc gcgccgcgat ccgacgatca acgcgatgcg ttcgggatcg
      601 gcgccggctt cgacgaggcg ggcgaaacgc tcctggagga cggcggtctt gcccgtccct
      661 gaaccgcccg tgacgagcat cgcgccgtcg gtgtgctcga gggtttcccc ctgccggggg
      721 tcgggatcga agggggccat cgaggcgatg ctaccgccgc gctcggacac gctcgccggg
      781 cacgatggtc agctggcgcg cgccgcccat cggtagacgc cggagccgct gatcgcgcca
      841 acgagctgcc cgtcccggag caccagcgca gcgcgttcgg tgcccagccg taccgagacc
      901 tcgtcgagcc gctcgtcggg gtgcgcgatc aacacgtgct cgagcgggat gaccgcgtct
      961 cgcgcgggcc gaagcggatc gcgcatcccc agctcgcgag ccgagctgaa cgagatcatc
     1021 ccgatcacgt tcccgtcctc gaggaccggg aacgcctctt cctcgtggcc ccgcaggaac
     1081 cggtcgagcg tctgcgacag cgagagatcc gccgggaccg tgggcggtgg gggatccatc
     1141 gcatccgcga cggtcgcgga cgcgaggcgg tcgtcgatgc cgatgcgctg ctcggaggcc
     1201 tgggcgccgc ggaagatgaa catcccgatg atgccacccc agacggcctc gaagagctgc
     1261 tgccgggcga gcgcgaagat cgccaacgcg accacgatcc cgcccaccac gagaccggca
     1321 cgcgcggcga cgcgcgtcgc cttccgctgg tcgccggtca cgcgccacac gaccgattgc
     1381 agcatgcggc cgccgtcgag cggcagcccg ggcagcgcat tgaagacagc catgaacagg
     1441 ttgaccgcgc tcacgtagct gaacagcccg gggagcggac cggtcgtgga ctccgtcagg
     1501 cgcgagagga tccagaacac ggccgcgagc gcgaggctcg tgcccggtcc cagcgccgcg
     1561 atcgcgaacg ccggtccggg acccttctcg tcagccctgg ccgccgtgaa ccctccgaag
     1621 aacaccagcg tgatgccgtg gacctcgatc ccgttggcgc gggccgccac ggcgtgggcg
     1681 gcctcgtgca ggaacaccga gccgaagaac acgagcgccc cgaacagggc gtagccgacg
     1741 atctcggccg aggccaggtt ggggaagtcc cggtcgagct gagcccagaa cgtcgcgacg
     1801 agcagcacgg cgatccagat ccaggaagag tcgacgttga cggggacccc cctgatcgtc
     1861 gcgatgcgga acccgcggcc gaacatgctc cgagggtacc cgcaggcgga tcggaaggcc
     1921 gttctgcgac ggtctgcgac agtgggggcg tgggcgatcg cgtcgaccgg ccgaccgaac
     1981 ccgaaccgac gcatcgcgtt cggaaggctg cgaccgtgct cctcgcgtgc gtcgtcgggc
     2041 tcgtgggcgc gtggctcggc atggcgctcc tcgcgcgcga caccgttccc gtcgggccgt
     2101 tccgggtgga gctcgacgtt gggttcggcc gcggggagac cgtgctcggg ctgccgccgt
     2161 tcggcgacct gaccgcggac acgcacctgt ctccgctgca tgtttcggcg acgctgaggg
     2221 atgtcggggt tcagcggctc accgacgtcg tgaacgacga gggcatcgac gggctcgtga
     2281 gcgatgtcga acgcgatgcg caagatcggg ttcgaacgat ggcgtggcgg gtcctcgtcg
     2341 tcggcaccct gggaggcgcc gcgttcgcgg ggctcgtgta ccggaggcgg tggcgcctgg
     2401 tgacggccgg ggcgatcgcc gctctcctcg gtgtggtgct gagtgaggtc ctcctcctgg
     2461 cgacgttcca accagcggct ttcgcggagc cgacgtactc ggggtcgctc gcgctcgcgc
     2521 cgcagctgat cgggccggcg cgcgaggcga ccgaccgtgt cgaggacctg cgggccgggc
     2581 tcgaggagat cgtcgacggg gcggtccgcg cctacacgac gttgcaggcg acgcccctgg
     2641 ccgacgacgc gatccgcgtg ctccacatct cggacatcca cgcatccccg ctcggcatgg
     2701 acttcgctca ggaggtcgcg cgtgggttcg acgtcgactt cgtgatcgat acgggtgaca
     2761 tcacgtcgtt cggaacgccg gtcgagaacc tgatcacgtc gcagatccct ggctttggac
     2821 ggccctacgt attcgtccgg ggcagccacg actcgatcgc gctccaggag gcggtggcgg
     2881 accagtcgaa cgcgatcgtc ctcgacgggc gagcgcgcac gatcgaaggg gtgacggtct
     2941 acggtctggg gcatccggcg ttcacgcccg cccgcggcgc gccggtggat gccgaggact
     3001 tcgcggcgtt ggcgcgctcg gccgggccgg tgatcgccgc cggtctcgag gcgtcggcgg
     3061 agcccgtaga tgtggtcgct gtgcacgacg accgcatggc cgaggcggtc gccggccggg
     3121 tgccgctggt gatctcggga cacttccacg acacggtcgc atcggtcgag gacggcacgc
     3181 tctacctacg gatcggaacc acaggtggct cgggtgccgg catcttccgg gatctcgccg
     3241 acatcccgtt cacggcggag gtcctctatt tctcccgagg agaggatccc gaactgatcg
     3301 cgtacgacgt gatcgagcag ttccccgaat ccgggagtct gacggtccaa cgggtaaccg
     3361 tcccggtgga gttcggggag ctcgtgctca cgccgacccc cgcgccgacg ccgagcgtgt
     3421 cgccgtcggg cgcgtcgccc tccgcctcgc ctgatccgaa cgcgacgtga tcggagggat
     3481 caagggtctc gaggaggaac gggtgggctc gatcgcgcgg gatcctgcag gctcgaatca
     3541 ccggcaccgg tcaatcttgc cgtcgctgtt acagtcgatg tgggtactcc ggaacccatc
     3601 ggagcggggt ccggagggag tagcgtccgg cccggcctac cctcccttcc acgcgggcgt
     3661 gcccgcgggg tcgggccgga ctctagtccg gccgaggctt actcctccag aaggtccgcg
     3721 agctgcctcg cgttgttcac cgcgaagtcc cggtagcagt tgttcatcag gacgtgggtt
     3781 tcgcgcgact ggtccgagat gtcgtggatc ctcgggaccc actcctccag ctcctgctcc
     3841 gagtactcgt agcggaaccg ctcggacgcg ctgacgttct tcttcgccca ggccttccgg
     3901 tcacgcccgt ggaaccgcac caacgcgaga tccttcgccg tcgccgccgc gatcggcggg
     3961 atgctcgaat cgaacccctg tggcatgtcg acgcagacga gcggcaggtc gtgttcctgt
     4021 aggaacgaga gcgtctcctc ggcgttgcgt tcttcgagcc acgtcttgtg ccggaactcg
     4081 atggccagac ggaagtcctt cagccgctcg gcgcactcga cgatgtagtc cttgttcttg
     4141 cgcccgatca cgaaccactg tgggaactgg aagagcacgg ctccgagctt gcccgacgag
     4201 tggagcggca tgagcgcgtc gtggaaccgc tgccagacct catccaccgc ggcgtccggg
     4261 aggttctccc gatacaggtt cttcttgtcg tcgagctcgt cggggagctc ctcgagcagg
     4321 tccttgtaga gggattcgcg cttggtcggg tggttggtga gcagcgagta cgccttgatg
     4381 ttgaacgtga agtcgttcgg cgtccggtcg atccacaaga cggcgttctt ctcactggga
     4441 gggaagtagt aggtggagtc gacctcgacg atcgggaact gcgtggcgta gaagcgcagt
     4501 cgagcctcgg cgctcttggc ctccggcgga tagaagtgcc cgtccttcac gagggtcggg
     4561 tccgtccagc tggccgtgcc gacgaggatc ttgctcacgt ccccagccta cgggcccgag
     4621 ccgatatcgg acggcgacgg ccggcatggc gcagtggata ccaaacgcca gctccggcct
     4681 cgatttgtcg ttcttgaccg cggaaacgca aacacccaaa gatggtcttc gagcggaggg
     4741 aggtcttatg gctgccacga ctgccgtcgg cgcaggtgga gttcgccgtc gacctccacc
     4801 tggaacggct ccgattgtcc gaggcttcgt gctcgggacc gttctacttg accttgcaat
     4861 cctgacctat ggcgcgatta cttcaagcga cgggtttcgt gatgcgggct ggggcctggc
     4921 tgcatgggta gtcgtcgtag cggcagtcgg cgcagcatct ctgccgttcg agtctggtca
     4981 ggcgcttggc ctggatatgc ccgttctact gtctgttggc tacctgttcg gcccggtagt
     5041 tgcgggagcg accgcgtttg ttgcctacgt tgacgcccga gagttgcgcg gtgagattcc
     5101 tctggtgcga gcggtgttca accgtgccca aacatctttg agcgtcatgg ctgcgactgc
     5161 ggtctttggg gtggtggtca gcggtgggga tggctggcca atcgctgttg ttgccgcatt
     5221 cctagccgtg ggaactgact gcttggtgaa ctacggaacg gtagtggcgg ttctctgtct
     5281 acacgagcgg atcactccgt gggacggtct gtcacgcctc tactttgggt ttttcccgga
     5341 gtttgcgctg acatacgcca gtttcggtct cttgagcctg atgctggcgg agatctacaa
     5401 gtctgtaggg gcatggagtc tcgcggtgtt tgtgatcccc gtcctgctcg ctcgtcaggc
     5461 gtttgctggt cacaagagac tggctagcgc ggcgaggcgc cttgacactc aagccgaggc
     5521 actacgagat gtagcggcat ctgttgcaga cgaacgcagg gaggagcgcc tctccttggc
     5581 ggcaggcctt cacgatgagg tacttccgcc gctcttcaag gttcatctaa tgggacaggt
     5641 acttcgtcag gatctggcgt caggccgctt gctcgcgctg gaagaggatc tacccgcgct
     5701 tcttggtgcg accgagaacg caagtgacag tatgcgtgag ctaatcagga gcctcaggaa
     5761 ttcgcccctc ggtacgggag gcttgcccga tacgctcagg ctcttggtgc ggtatctgga
     5821 acgtgagtca acggcgcgaa tcagtctcag ctgcgagcgt ataggtggca cgcccttgat
     5881 ccagctgctc gcttaccaag tcgcacgaga ggctatacgg aacgctctga agcatgcgag
     5941 ggcatcagag atcaatattc acgtcgtgga cgtggagggg aatctccgaa tcactgtgga
     6001 agacgatggg atcggtttcg aacccgactc cgttgatcaa caggtccact ttggcttggc
     6061 gcttatgcga gagcgaatcg agattgcggg cggagtcctc tatgtcgatt cgcggttggg
     6121 agaaggtacc cgaatcattg cacggcttcc ctccaaccac gagcagtcga agggcgggcc
     6181 tcccaaggag acccgcccgt gaatccctct gttccgccgg tactagaaac ggagatgtgc
     6241 gccaccagcg agcgcgagcg ccgccagcga gccaaccgca acggcgagcc gcttgatcag
     6301 cttcatggca cctccctccc cgtctcgagc gagctgtccc acttccctgc catgtggtac
     6361 tccctcgctt gccggacccc tatttggggg acatcggggc ccactatcgg cgtccccctt
     6421 ttgaccctca agccgcaaat gtcccctaca gtgcttgctg taggagcttg acgaaaccct
     6481 cgtgccgccc agcttcatcc aatagggtgg tttcgccgct attcgcgacg gttcaatatt
     6541 gaaagccgaa tcgggcagga gaccggacga tgtcatcgga tcacggttcc atcaaagcag
     6601 ccaaatccgc gaggctcttg cccttcttcg aggcggcagt ggtgattccc accttgggtg
     6661 cactcacatt tgccatcgcc agggatggac tggccgaaga gtcagaactc ccccagctgg
     6721 tgggctggat cctgatcatc gctttggtcg aattaatccc ggtaccagtc tggcggggcg
     6781 ttcacatcgg cttggggttc cctctgatga tggctgttgc cttcatgtac ccaccccagg
     6841 ctgctgctgc ggtcgccctt ctcgcggcat cagatccacg cgaattcaga cgagaagtgg
     6901 gtctgctgcg ggcgctgttt aacagatgtc aggtggccct ggcggtagga agtgccagcc
     6961 tcatcttcta tgagctggcg gacctcgata cgtccagtcc agttgaactc attgctgtcg
     7021 caggtcttgc ctcgattgct gactatgtgg tcaacacaac tctcgtgtgt gcggcagcca
     7081 gcatccattt ctggatgaat ccgctccgag tcatacggga gttgaaggtt ggtcgcttaa
     7141 gcgaatatct ggtgagctac ctcggcctct cagtctttgg tctcatgctt gcgaagttct
     7201 taagcggacc ggggaacaac tggtgggctg taccggtatt ccttctgccg cttctccttg
     7261 ctcggcagat gttcttccgt agcaaggctc tcgaggaggc gcacaaggaa ctccaggatc
     7321 gcgagcacgt acttcgtgca ttgtcgaacc gcatggcgga ggaacgtcag gacgagcgca
     7381 tgcagatcgc cgcgttcctc cacgacgacc ttgcgcaact cctgttcaga ctctcgatcc
     7441 aggtcgacgt ggctcgaagg catctcgacg cggggaaggt cgaccaaaca gaggagttgc
     7501 tcctcgagat caaggagacg aagaaccgaa cgtccgatcg catccgggcc ttgatccggg
     7561 acctgcaccg gtcgcccctc gggcgggcgg gtctgggaga ggcgatccgc gggttcctcg
     7621 tcgagatggc gcgcgaatcc gacgttcgac tccacgccga tatcgacgag gtggacgtgc
     7681 ccgcgccgat cgcactgctg ctgtaccaca acgcacgcga gggtgtgatg aacgccctca
     7741 agcatgcgaa ggcgtcgaac atctgggtcg cggtcgagca ggagggtgac gaggtcgaga
     7801 tggtcctccg ggacgacggg gtcggcttcg acgtcgaggc gccgggtccg gaagggcact
     7861 acgggatgac gatgatgcgc gagcgcgcga ccgtcggcgg cggaacgttc gccatcaaga
     7921 gtgttcccgg agaagggacc acgatcaccg tccggttccc gacgtcctgg ctccaggagg
     7981 aagaaagcca aaatcaggct caacaacagg ctcccgcgtt gccgcccgcc ggtgcttccc
     8041 gcggcgcggc ccctcccgcc tcgccgacgg agtccatccc cgcatgaccg cgacgatcaa
     8101 ccagatcagc ccgacgtaga cgaacacgtc tcccacgctg atcgcctggc cgatcggctg
     8161 ggggaccggg atgacgtcgg cgaggaaggt cagccggtct tcgtccgtga gcagatggtg
     8221 cttctgggcg tggccctcct cctggaggta cgcgagcacg tcctcctggc ccgaatcgat
     8281 cagggcctcc gcgctggccg gcatcccgcc gttcacggcg atcaccgcga agttgcaggc
     8341 caggccgatc accaccagga acatcccggg catcc
//
LOCUS       DASUYD010000030         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1337591, whole genome shotgun sequence.
ACCESSION   DASUYD010000030 DASUYD010000000
VERSION     DASUYD010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1337591"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF195_01470"
     CDS             <1..332
                     /locus_tag="VF195_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017932813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6579533.1"
                     /translation="ALDHDRIFGALGDATRRDIVRRAIDGEEGVAELASHYPMSFAAV
                     QKHVAILERAGLVSKHRIGRRKVVRTNPERLRVARRLLDRYEELWRERVERMDRLISG
                     SPKEAER"
     gene            371..834
                     /locus_tag="VF195_01475"
                     /pseudo
     CDS             371..834
                     /locus_tag="VF195_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
     gene            860..1495
                     /locus_tag="VF195_01480"
     CDS             860..1495
                     /locus_tag="VF195_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6579534.1"
                     /translation="MKLTTTTMVTVDGVMQGLGGPDEDRRGGFERGGWVTPYTDKEGM
                     GFLNEVYGRADAFLFGRRTYEIFADSWGTWPDPGDSPIWTALNTRPKYVASTTPFDPK
                     WANTTVLSGDVAAAIRELKAKAGGELQVHGSGVLVRWLLDNQLVDEINLLTLPVVVGQ
                     GTRLFPDTGPDIALELVGSQAVTPSGVTIQVYRPTGRPQYATASADVKHES"
     gene            1558..1794
                     /locus_tag="VF195_01485"
     CDS             1558..1794
                     /locus_tag="VF195_01485"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6579535.1"
                     /translation="MLINLPATRTGGIWRGANIMRQYLEAGLLDEMQIHLIPILLGGG
                     VRLFEDLDPEGIELRRTSSIETPGATHFRFEVVK"
     gene            complement(1971..2159)
                     /locus_tag="VF195_01490"
     CDS             complement(1971..2159)
                     /locus_tag="VF195_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579536.1"
                     /translation="MGKGSTGAAAGGGAIYGLGIFGALFYFWQQADAFWEYVWAVFPK
                     AILWPAFMVYEGFRALGA"
BASE COUNT          447 a          807 c          817 g          356 t
ORIGIN      
        1 tcgcgctcga tcacgaccgc atcttcggcg cgctgggtga cgccacccgg cgtgacatcg
       61 ttcgccgcgc catcgacggg gaggagggcg tcgccgagct ggctagccac tacccgatga
      121 gcttcgcggc ggtgcagaag cacgtcgcca tcctcgagcg ggccggcctc gtcagcaagc
      181 atcgtatcgg gcggcggaag gtcgtccgca cgaaccccga gaggctccgc gtggcgcggc
      241 gcctgctcga ccggtatgag gagctgtggc gagagcgcgt cgagcgcatg gaccggctca
      301 tcagcggatc gcccaaggag gcagaacgat gaccgtcacc gccgtccgaa aggatccttc
      361 gtcgcagacg atgacgctgg aggccgagtt tgacgcgtcg ccggagcggg tctggcagct
      421 ctgggccgat ccgcgccagc tcgagcgctg gtgggggccg ccgacctacc ctgcgacggt
      481 cgatgctcac gacctgcgct ccggcggcca tgtcgagtac cgcatgaccg gcccgaaggc
      541 gatcagccgc gcgggttctg ggaggtggac gaggtccagc caccgcatcg gctcgtcttc
      601 cgcgatggct tcgcgaacga ggacggcacg ccgaacaccg agctccccat gaccaccgcg
      661 cgggtgacca tccaaccgat tgatggcggg aagacccgga tgtcgatcga gaccgagttt
      721 ccgagcgtcg acgcgatgga gcagctcgcc gccatgggca cggaggaagg ccggacgcaa
      781 gccgtcgggc agatcgacgc gatccttgcc gaggaacgcg atcggtctcg ttgagaagcc
      841 aacgagaaag gagtcgacca tgaagctgac gaccaccacg atggtcaccg tcgacggagt
      901 gatgcagggc ctcggcgggc cggacgagga ccgcaggggc ggattcgagc gcggcggatg
      961 ggtcacgccg tacaccgaca aggagggcat gggcttcctg aacgaggtct atggacgcgc
     1021 ggacgcgttc ctgttcgggc ggcggaccta cgagatcttc gcggactcct ggggaacgtg
     1081 gcctgatccg ggcgacagcc ccatctggac ggcgttgaac acgcgaccca agtacgtggc
     1141 atcgaccacg cccttcgacc ccaagtgggc gaacacgacc gtcctctccg gtgacgtcgc
     1201 ggctgccatc cgcgagctga aggccaaggc gggaggcgag ctgcaggtgc acggcagcgg
     1261 cgtcctggtc cgctggctgc tcgacaacca gctcgtcgac gagatcaacc tgctcaccct
     1321 ccccgtggtc gtcggccagg gcacgcggct gttccccgac accggccccg acatagcgct
     1381 cgagctggtc ggatcgcagg cggtcacccc cagcggagtg acgatccagg tctaccggcc
     1441 caccgggcgc ccgcagtacg caacggcctc ggccgacgtg aagcacgagt catagatctc
     1501 atcgaggggc cggacgaccg ccgatcgatc cgagggacag atggggtcaa tgcaggggtg
     1561 ctcatcaacc tgccggcgac aagaacgggc ggcatctgga gaggcgcgaa catcatgaga
     1621 cagtacctgg aggccgggtt gttggacgag atgcagatcc acctcatccc catactgctc
     1681 ggcggcggcg tccggctctt cgaggatctc gaccctgagg ggatcgaact gcggaggacg
     1741 agctcgatcg agacaccggg cgccacgcac ttccgattcg aggtggtgaa gtgacacacg
     1801 ctatcgccca cttgtcgcct tcctttgggt gcggttgacg tgaagatgtc gtctcgggat
     1861 agagccgccc cgagtcgtga acaagatgac gcacatgaga agcgtcgcgt tacgtccgct
     1921 cttgcaggtc cccccgcaga tccagcgtct gacgaggacc gatgcctccg ttacgcgccg
     1981 agcgccctga acccctcgta gaccatgaac gccggccaga ggatggcctt cgggaagacc
     2041 gcccagacgt actcccagaa cgcgtcggcc tgctgccaga agtagaacaa cgcgccgaag
     2101 atcccgagcc cgtagatggc tccgccaccc gcggccgcgc cagtgctgcc cttgcccatc
     2161 gccgacctcc tccctcgtcg gtcttgcgca cgaggctatc tcgagcccat cctcacaccc
     2221 tcgatgggat aggacatcgc gataggttgg aagggtcgac tcgaaggcgg ctcagcaccg
     2281 atggaccagc gcagactcgg gcgcctcggc cacttgagac acggaacaag ggtcttccgt
     2341 gtggcgcgtc agccgttggg catgccgagc gctgcgcgaa gctcaccgag catccgctgc
     2401 tcgccctgac tgaccagctg cgcgccg
//
LOCUS       DASUYD010000031         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1560654, whole genome shotgun sequence.
ACCESSION   DASUYD010000031 DASUYD010000000
VERSION     DASUYD010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1560654"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..137
                     /locus_tag="VF195_01495"
                     /pseudo
     CDS             1..137
                     /locus_tag="VF195_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409254.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            322..1140
                     /locus_tag="VF195_01500"
     CDS             322..1140
                     /locus_tag="VF195_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX6579537.1"
                     /translation="MGHSNAPLTPEGRRRLCERVDAGRAICHVAAEAGIARQTLGKWY
                     ERWITEGDQGLVDRSSRPECSPNQTAVHIEDRVEALRRERKLGPVQLMGLLAGEGIEI
                     AASTVYRILVRRGISRLRDLDVSGEDLREPVVRYEWARPGDMVHVDVKKLGRIPSGGG
                     HRVHGRTSAQYRATRRAKTSGARAGFVFLHSALDDHSRLTYTEELPDELGSTAAGFWA
                     RAVKFFHHHGIRRIRRVLSDNGACYRSRAFALALGASRSRHKRTRPYRPQTMRV"
     gene            1311..2387
                     /locus_tag="VF195_01505"
     CDS             1311..2387
                     /locus_tag="VF195_01505"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6579538.1"
                     /translation="MRTVDLHRRAAREGGPVFVGIDSHKDSLAACAVDGNGREVRSAV
                     FENSPAGHRDLLAWAQRTGTLERFGIEGSANLGAGLTRLLMAEGETVCEVPCEMTVRE
                     RRRLRRPGKSDPADALAIARITAREATLPPARRAGATEDLKVLSDYRDQLVVERNAEV
                     NRLHADLAIVCPGYARRCRTLVAARSLLTAGRLLRRETSVRAQVAKRRISRLRKLDRQ
                     IAELAKEIADLVQETGTRLTEIVGIGPLLAARILGEVADVSRYPSANHFASANGTAPI
                     PASSGRTDRHRLNRGGNRRLNKALHYVALTQANFEPRAITYLARKQSEGKTRREALRC
                     LKRRLSDVVYRALVSDAQEGAMAA"
     gene            2411..2593
                     /locus_tag="VF195_01510"
                     /pseudo
     CDS             2411..2593
                     /locus_tag="VF195_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            2728..3942
                     /locus_tag="VF195_01515"
     CDS             2728..3942
                     /locus_tag="VF195_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6579539.1"
                     /translation="MIFVGIDWSEDHHDACIVDGDGRVLARGRVPEGVDGVAKLHEMV
                     AAHAEDPSQVTVGIELDRGLLVGALVASGYAVHAINPLSVDRYRDRHRTSGAKSDPGD
                     ARVLADLVRTDRHLHREVAGDSELVEALKVLARAHQSLIWSRQRQLNQLRNALREFYP
                     AALEAFGTDLASTDALAVLGVAPTPAEGRSLPAARIAAALKRGGRRRHITARAKEIEA
                     ALRSTQLEAPAVLARAYGDVVRSTVHVVAEMTAQIESLAGELDASFEGHPDAEILRSL
                     PGLGSVLGARVLAEFGDDPDRYADPKARKNYAGTSPITKASGRSKVTLARFARNRRLA
                     DAVEMWAFCSLTLSPGARRYYDAHRAKGHTHRRALRSLANRWVGILHGCLRHREGYSE
                     ARAWPTCAEAAA"
BASE COUNT          651 a         1415 c         1379 g          644 t
ORIGIN      
        1 tcgacgaggg tgaggggccg ccgctcctgt tcgtccatgc gggaacgggg tcgttcctat
       61 ggcgcgacgt gatcgtccgt ctccgcgaac ggttccgctg cgtcggactg gacgttctgg
      121 gcggcggcct gccgcaggtc atcgagggcg aaccgctcga cctcggtgcc ctctcgcgga
      181 tcctcggatc gttcgtccgc gcactagatg ttctaccccg gaaggttggt gacacggatc
      241 gagcagcgga gttgacgtag gagagcctcc ccgcgtccga tgtgcagttc gcagccgcgc
      301 acggacgaga aggaggctct cgtgggtcac agtaacgccc cgctcacccc tgaaggacgg
      361 cgtcgcttgt gtgagcgcgt cgacgccggc cgagcgatct gtcatgtcgc cgcggaagcc
      421 gggatcgcac gccagaccct gggcaagtgg tacgagcggt ggatcaccga aggcgaccag
      481 ggcctggtgg accgctccag ccgccctgag tgctccccca accagaccgc cgtgcacatc
      541 gaggatcggg tcgaggcgct tcgtcgcgaa cgaaagctcg gaccggtgca gctcatgggc
      601 ctgctcgcgg gagaagggat cgagatcgcg gcctcgaccg tctaccggat cttggtacgt
      661 cggggtatct cccggctccg cgacctcgac gtctcgggtg aggatctacg cgaacccgtc
      721 gtccgctacg agtgggcccg tcccggtgac atggtccatg tcgacgtcaa gaagctgggg
      781 cggatcccat cgggcggcgg ccatcgcgtc cacggccgca cctcggccca gtaccgagcc
      841 acgaggcgag ccaagaccag tggagctcgg gccggcttcg tcttcctcca ctcggccttg
      901 gacgatcact cccgcctgac ctacaccgag gagctgcccg acgagctggg ttcgaccgct
      961 gcggggttct gggcccgggc ggtgaagttc ttccatcacc acgggatccg gcggatccgc
     1021 cgggtgctga gcgacaacgg cgcctgctac cgatccagag ccttcgcgct ggcgctgggc
     1081 gcgagtcgaa gccgccacaa gcgcacccga ccctaccgtc cccagaccat gcgtgtctag
     1141 atgtcgttgt gtcggagtcc ggtctccgag acgctgaccc ggagcccgag ggtggctccc
     1201 gcagacgatg cccgggccct agcccgaggc cacgcccgtt gagtgccccc tcgggcgcat
     1261 ccgggtcgga cggtgtcgcc tgataggagc gtgatgttct cgacgcatgg atgcgcaccg
     1321 tcgacctcca tcgtcgcgcg gcccgggaag gaggtccagt gttcgtcggc atcgacagcc
     1381 acaaggactc gctggccgct tgcgcggtcg acgggaatgg acgtgaggtt cgctccgcgg
     1441 tgttcgagaa ctccccggca ggacaccgcg acctgctcgc ctgggctcag cggaccggca
     1501 ctctcgagcg gttcgggatc gaaggttcgg ccaacctcgg ggcggggctc acgcgactgc
     1561 tgatggcaga gggcgagacc gtctgcgagg tgccctgtga gatgacggtt cgagagcgtc
     1621 gccggctccg tcgtccgggc aagtccgacc cggcggacgc cctagccatt gcccgcatca
     1681 cggctcggga ggcaacgctt cctccggctc gccgagcagg ggcgaccgaa gatctcaagg
     1741 ttctttccga ctaccgtgac cagctggtcg tggagcgaaa cgcggaggtc aaccggctcc
     1801 acgcggatct cgccatcgtg tgccccggct acgcccgccg ctgccggacg ctggtggctg
     1861 ctcgatcgtt gttgacggcc gggcggctcc tgcgtcggga gacttcagtg cgggcgcagg
     1921 tggcgaagag acgaatcagc cggctccgga agctcgatcg gcagatcgcc gagctcgcca
     1981 aggagatcgc agatctggtc caagagaccg gtacccgcct gaccgagatc gttgggatcg
     2041 gaccgctgct cgccgcgcgc atcctcggtg aggtggccga cgtcagtcgc taccccagcg
     2101 ccaaccactt cgcctcagcc aacgggactg cccccatccc agcttcctcc ggtcggaccg
     2161 atcgccaccg cctcaaccgc ggtggaaaca gacggctcaa caaggccctg cactacgtgg
     2221 ccctcaccca agcgaacttc gagccgcggg ccatcaccta cctcgcgcgc aagcagtcgg
     2281 agggcaagac ccgccgcgag gccctccgat gcctcaagcg ccgtctctcc gacgttgtct
     2341 acagagcgtt ggtctcggat gcgcaggaag gagcgatggc cgcttgacat aggagctcaa
     2401 cggcaaggtc gagcgctacc accagaccct ggctcgcgag tgggcctact cccaggcctg
     2461 gtcaaacaac gatgaccggg ctgctgcgct gggtgcgttc ttggcccgct acaactacca
     2521 tcgaccgcac acggcgctgg gaggcaagcc tccgatcacc cgctgcccgc gtgtcagcaa
     2581 cctcgcggct tagaacacct agatcgtgcc ctcgccggca ggcgaggagc cgacggctgg
     2641 tgggatgtcg tgccgatcga gcactggtcc attagggcgc cgcgtactcc tccaggctca
     2701 agatcgtcct cgagggaagg aggaggggtg atcttcgtcg ggatcgactg gtcggaggac
     2761 caccacgacg cctgcatcgt ggatggggac gggcgggtgc tggccagggg ccgtgtgccc
     2821 gagggcgtcg acggagtggc gaagctccac gagatggtgg cggcacacgc cgaggatcca
     2881 tcacaggtca ccgtcggcat cgagctggat cgggggctgt tggtcggcgc cctggtcgca
     2941 tccgggtacg ccgtccacgc catcaacccg ctgtcggtgg accggtaccg ggaccggcac
     3001 cggacctcgg gagccaagtc cgacccgggc gacgcgaggg tcctcgctga tctcgtccgg
     3061 accgaccggc acctgcaccg agaggtggcc ggggactccg agctcgtcga ggcgttgaag
     3121 gtcctggcca gagcccacca gagcctgatc tggtcccgac agcgtcagct gaaccagctc
     3181 cggaacgccc ttcgggagtt ctaccccgcc gcgcttgagg ccttcgggac cgacctcgcc
     3241 tcgactgacg ccctggccgt gctgggggtt gcacccacgc ccgcggaagg acgctcgctc
     3301 cccgcggctc gcatcgccgc cgctctcaag cgaggtggac gccgccggca catcacggcg
     3361 cgggctaagg agatcgaagc ggcccttcgg agcacccagc tcgaggcgcc cgcggtgctg
     3421 gctcgcgcgt acggcgacgt cgtgcgctcg accgttcacg tcgtggccga gatgaccgcg
     3481 cagatcgaat ctctcgccgg cgagctcgat gcgagttttg aaggacaccc ggacgccgag
     3541 atccttcgca gtcttccagg actcggctcg gtcctcggcg cccgggtcct cgcggaattc
     3601 ggggatgacc ccgaccgcta cgccgatcct aaggcccgca agaactacgc ggggacctct
     3661 ccgatcacca aggcctcggg gcgatcgaag gtcaccctgg cccggtttgc ccgcaaccgc
     3721 cgccttgccg acgccgtcga gatgtgggcg ttctgttcgc tcaccctctc tccgggagcc
     3781 aggcgctact acgacgcaca ccgtgccaag ggtcacaccc accggcgagc tctgcgctcg
     3841 ctcgcgaacc ggtgggtcgg catcctgcac gggtgccttc gccatcgcga gggctactcg
     3901 gaggcccggg cgtggcccac ttgcgcggag gccgcggctt gacacttacg cccgtgggat
     3961 gtctagcgcg tggggtcgcg ctcggtcgag gacgttcgtt cctcacgcca tcgtgtcgac
     4021 gatcaaccaa ccgaaccccg tgccccacac cgcgacggat acaggcgccg tcaccctgac
     4081 gttccgggg
//
LOCUS       DASUYD010000032        33758 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1120468, whole genome shotgun sequence.
ACCESSION   DASUYD010000032 DASUYD010000000
VERSION     DASUYD010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 33758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33758
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120468"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="VF195_01520"
     CDS             complement(<1..1203)
                     /locus_tag="VF195_01520"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6579540.1"
                     /translation="MRTSISKFNLFGLTILALVLAGCTAEPTGSPSTGGGTQAAAGPA
                     GVEVTLSEFAIEPTALSVPAATPLEITVMNHGQAAHTFGVTIGDQTIETSTIDPGGSA
                     ILSVPALEAGSYGTLCTVPGHADSGMVGTLTAGAGDVGAPAAHTTMTAEQMADGHAAG
                     VKAFVAGDQTDVQGNQPLQPKIDGGVKVFELWAENLQWEISKGVFKDAMAFNRQIPGP
                     EIRVRTGDRVRIFLKNLMDQPTVLHFHGLTIPNEMDGVPYVTQDPIMPGDSFYYEFRV
                     TDPPGMYVFHSHFNSAEQVGKGLYGAFIVEPPGGRWRSVYGVEPDVEADLFLGDGPLD
                     YTLNGKSFPATTPIVAERGDDVLVHLANDGSLLHPMHLHGFHFTVVGVDGFPLAPEER
                     YPADTLVVA"
     gene            1640..2890
                     /locus_tag="VF195_01525"
     CDS             1640..2890
                     /locus_tag="VF195_01525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579541.1"
                     /translation="MWQRGASRVGLAITCTVALARPAMATDGDLDPSFGEDGRVATVF
                     PVGSFATAVAIQDDGKIVAVGAAAGPSETGEFALARYEEDGGLDPSFGEGGMVTTAIA
                     GGGGDEARSVAIQENGRIVVAGADSWQRFAVVRYRPNGQLDDSFGGGDGIVRTNFTPG
                     DDMAWDVVLQRDGKIVAVGGAGLGHEGFQIARYQRDGTLDPTFGEGGKVVTRYLDANA
                     RAVALQPNGRILVTGTNVRWLALARYRPDGRLDRSFSGNGMVGVALGIYILPHAVALQ
                     RDGRIVVAGGFDYDHIGLARFLDDGRLDRSFGGDGIVRRKVEGEAQQAIGLILQPDGR
                     IVVAGWSGPHEAEPTVYRFIMIRVLPDGRLDQTFGDRGRVATFFDGGAIAYGAAADHE
                     GRVVVVGGAGEGNRDAFALARYVV"
     gene            complement(3137..3373)
                     /locus_tag="VF195_01530"
     CDS             complement(3137..3373)
                     /locus_tag="VF195_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579542.1"
                     /translation="MAEELMADDADAIIAEARKTKETAERMANGEGPPGEADDIRVLA
                     GMVHQVAEQVERLAGVVRGGEAPLAHSDEDDTDS"
     gene            complement(3580..4389)
                     /locus_tag="VF195_01535"
     CDS             complement(3580..4389)
                     /locus_tag="VF195_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003983558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX6579543.1"
                     /translation="MAEGDTVFATAARMHHALAGHVLIATDLRVPRYAAGDLSGQSVR
                     EVGARGKHLFVRTDGGVSVHSHLGMDGSWRLYEPGERWRGRGFEVRAVLTTEERIAVG
                     HRLRRLEVIRTRVEPELLAHLGPDVLGPDWDAAEATRRLVASPDREVGDALVDQSVMA
                     GPGNVYRSEVCFLAGVDPRTAVGSLADPGGIVELMRSLMDRNRAGGRRVTTDDPRPGH
                     DLWVYGRGGRPCRRCGTPIRSFARGPRSRERITFACPRCQPLVGPSALDDS"
     gene            complement(4683..5576)
                     /locus_tag="VF195_01540"
     CDS             complement(4683..5576)
                     /locus_tag="VF195_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579544.1"
                     /translation="MVRSQRGRHACLSLCFVVATALVYEIVTFGSVVAAPQWHLVRLI
                     RTTPFVNKAVSMKDAEGSAYVPRDHSLWLADDYGRRIYEVNPSTGALKRVIGQKKLAS
                     ARRLGGGVRAGIDRTRDLESIAYDRARDALYVFSGPCCSSSVKPTAFRLKRTGAGRFR
                     VESFQPLPRSANFTAAAWNAANHMIYVGQGRDLRSYRYPANDTGRLFNVPNLSGITGM
                     SFSPNGGSLFVTTTAEELHRVVWPRKRLAAEWVLELGSFGVGDARAVEYIDRRYYVLD
                     GDPARPDTDPRKYAVYVFRMS"
     gene            5697..6692
                     /locus_tag="VF195_01545"
     CDS             5697..6692
                     /locus_tag="VF195_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX6579545.1"
                     /translation="MRERTIIVTGGAGFVGSHLCDRLLAEGHQVVAVDDLVTGRTENL
                     SLASGHGDAFGFSETDIRTPGLQDVFDRHRPDVVMHLAAQAGVRPSLEDPDHDASVNV
                     LGTLNVLRCATSVGCRMVVYAASGGTLYGEPTSLPVRESSADGAHPESPYGISKKVVL
                     DYLRFFSRHRSLEFVALALGNVYGPRQDPTGEAGVISIFASSMLAGRSPTIFGDGEQT
                     RDYVYVEDTVAAFVAAMDRASGEIVNIGTGIETSVNALYSMLCEIVGIPAAPRYGPQP
                     EGELRRIALDNSHAASVLGWRPRTDLAVGLARTVEHLRESYSPSADARSDSDTEP"
     gene            complement(6644..7132)
                     /locus_tag="VF195_01550"
     CDS             complement(6644..7132)
                     /locus_tag="VF195_01550"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579546.1"
                     /translation="MEQLALEIGRALRRARLDRGMTLRGLADSSAGRFRPTSLAGYER
                     GERTISVVRFCELCRLLDVSPARLLQEITRAIYGHGEPRIDLTKLEALHEPEGELIAG
                     FIKQVLVQRADPDVETIALRAGDVEVLASAAGTTPAELHDIVSAAQGSVSESDLASAD
                     GE"
     gene            7607..9166
                     /locus_tag="VF195_01555"
     CDS             7607..9166
                     /locus_tag="VF195_01555"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6579547.1"
                     /translation="MSSVIESPSYALASASRPEEAAAHATSWVREILHGDVSVHIARL
                     DAAGRLRTVWRDGENSSTGSGLTTARRIALQTRAYSHFDFPRITGARRAALFPLVCRE
                     QSLGLLEIQADRAALEERSGILAAIACQLAGVLAGLDERDGLARQVGTLEELADIARI
                     LLRASGPEEALAIVASALSRDVRAPVAIWWGPDDGVRTLASLSGVNAAKTEVVIRDLG
                     EVPPSDQLSDGERAELRQRFARVLGVRGATIRDAAGALVLVASRSPFAQHRVDVIGSL
                     LEDTLPLLLASAREAKRSSYLDVGLAWTAHELRTPILGVKAALEGVVGRSGPRDESVL
                     RRSVSELEKLALDTEGILGWATGRRELDTSVVDVSRLVEDAIASYRVLHDVEATIVVD
                     ADPSVTASVDPVQLRAAVMNLIRNAALYSGRDAIVRVHVASEGTAVRVSVRDQGPGIP
                     QEERAAIFDPFVRGREGSRVRDGSGLGLFITRRVVEAHGGRIWVDPDDEGSTFHVLLP
                     VDGRELQRSAS"
     gene            9211..9828
                     /locus_tag="VF195_01560"
     CDS             9211..9828
                     /locus_tag="VF195_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579548.1"
                     /translation="MLEAGMEPVGLATSGRGALEHLERDRPDVILLDLGLPDRSGLAV
                     GREILEDWPEAKVVALTALDDQKTVEAAQVAGFCGYLSKNVPVSRFVASIEAVAAGQT
                     VFPHRAASRRTHSPTNDSVWLLVSQLTAREREVLELLVEGAGGAMIARRLGISRNTVR
                     THVQSILTKLQVHSRLEAATLAVRHRVVPVPSNVDVDVSTPFDRV"
     gene            9965..10660
                     /locus_tag="VF195_01565"
     CDS             9965..10660
                     /locus_tag="VF195_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579549.1"
                     /translation="MSEPSGTIRVLLADRHALFREAVRAVLESEAEIHVIGEASTGPE
                     AVAESERTLPHVAILDADLPMSNSPQTTAAIKANAPDCHVLVLSAREDYRGLVEVLDA
                     GASGYLTRESPLADLIHATRAIHRGDTLVPPTMLGPLLTGLLRRKRDHDEAFERITRL
                     TVREREVLALLAQGSDNDSIARALVISPQTARTHIQNILGKLGVHSRLEAAAFVTRSG
                     MLQNLVRAEGANA"
     gene            complement(10664..11647)
                     /locus_tag="VF195_01570"
     CDS             complement(10664..11647)
                     /locus_tag="VF195_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX6579550.1"
                     /translation="MTAPRAVVAGGAGFVGSHLCDRLLDEGWEVVCVDSLLTGARENL
                     SDAATEGRFSIQEADITKGLDIEGPVDWIFNLASPASPVDYLAHPIETLEVGALGSLA
                     CLRLAERKGASLLLASTSEVYGDPQVHPQPETYWGNVNPIGPRSVYDEAKRFAEAATM
                     AFHRTRGVPVRIIRIFNTYGPRMRRHDGRAVPTFIDQALRAEPITVHGDGSQTRSLCY
                     VDDLVEGIWRFLDSDLTGPMNLGSTEEVTMAELAELIRSLTGARSPIVYTERPVDDPE
                     NRRPDTSLANDRLGWSPQIPLREGLSKTIGWIRRDAPLDRSGEGETLPSPA"
     gene            complement(11644..13065)
                     /locus_tag="VF195_01575"
     CDS             complement(11644..13065)
                     /locus_tag="VF195_01575"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEX6579551.1"
                     /translation="MKVTVMGTGHVGLVTSVMFASIGHEVVGTDIDVEKIQLLRRGIP
                     PFFEPGLEEGMARESVSGRLSFTANAAEALRGTDVVFICVGTPARASGDANLLAMEES
                     AKVIAQHAPDDTVLVEKSTVPAGTADRVRTTLEREGAGRRFHVASNPEFLREGSAMED
                     ALKPDRIVVGVESPRSLEVLRRLYAPLLDDGVELIVTDIPTAELAKHASNAFLSLKIS
                     FANAMARICERAGADVTAVADVMGSDPRIGRAFLNAGLGYGGYCFPKDVAALRRLSER
                     LGYPFPLLAEVERVNDEAVEAVAAKVEDALWNMEGKRIVVLGLAFKPGTDDVRFSPSI
                     ALARRLLESGALVVGYDPHAGASAKEELPELELANDVYGAASGAHAIVVATDWEEFRE
                     LDLPKLRDVMTYPLVVDGRNLLDSDEMAEAGFWYYPTGRPAVEPTTIASLTSPETAPP
                     ARVEQPPVEQRPAEQGPAEHRRT"
     gene            complement(13178..14845)
                     /locus_tag="VF195_01580"
     CDS             complement(13178..14845)
                     /locus_tag="VF195_01580"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX6579552.1"
                     /translation="MSRMFQEPPSVAPASGDGSLDLREFMGTVLRRKWLVLGVAIVVV
                     ALAAAYSYSRPKVFSSTASVVARPIRVQPTDEDPSDNLNMLTEAQMVRSTPVADIART
                     LIGRPQDLQDMLRRVTVTIPENTEILQISYTDSTPKRAQSGAQAFADGYLQFKGELAR
                     DGIDEDAARIQAEIDQLDLAMRQLEIDMQGMEEGSPELAAAESDRVSLEDTRLGLRNQ
                     LVTTIISSRDPGEVLIAAERPSSAISPKHQVDLALGVLLGIAAGMALASVRERMQDRI
                     ETPATLRNVLRAPTLGAIPDSQLLRGDLPRLVTIDERRSSPAEAYRALRANVLAAARD
                     AKARTILVTSALEGEGKTTTAVNLAVALAQVGKSVVLISADLRFPRVHVLLGIGNEQG
                     LGQVLQGSIELRDALVETPIQNLKVLPTGPVTADDEPVELLQSDRMVDVIRGCRQADV
                     VIVDAPPIGPVADSLMLVDLLDAVIVVADAQEGTRARVAEARHQLAQVGGNILGGILN
                     RIPESMTQPKYGSYNGRRTVNRYDDADRELAVLREEASRSSKTSSRA"
     gene            15192..16676
                     /locus_tag="VF195_01585"
     CDS             15192..16676
                     /locus_tag="VF195_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579553.1"
                     /translation="MADGVLVLIAALCRSLEAERVRYCHWKSNESIARSASGENDLDL
                     LVARADAERFERILRRLGFKDTDQPSWKRLPGIYHSYGLDPTGVFVHIHAHYQLVIGD
                     DMTKNIHLPIEAPYIGSARREGIFPVPSTAFELLLFLIRMVVKHSTWDAFVSFQGSLS
                     KSERRELEDLVERTDLHEVWELAERHLPFIPGELLERCLRCVQPDASRWLQIRTAARL
                     QRVLAGCSRRPPVLDTGLRMWRRGRTVLRRKVVRRGPVRSRMSSGGALVAIVGGDGAG
                     KTTVVDGLSSWLASEHIDSTTMHMGKPSWSALTAIVKGSLKLAAATKRTSTSSAKALR
                     SSLSAGEGGLSVRNRARLVWEVLTARDRFRTYRKARRLATNGAIVICDRYPLPEVSLM
                     DGAVTGRLKDPAAWGRWAAWLAGLERRYYERIRDPDTLIVLRVDPDTAVERRRGIEPE
                     SSVRPRSEEIWGIDWSGTSAIVIDAASPKDDVLSEVKATIWSRL"
     gene            16750..18285
                     /locus_tag="VF195_01590"
     CDS             16750..18285
                     /locus_tag="VF195_01590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579554.1"
                     /translation="MVSRVVSDGSLTKKASLNAAASTAEQGARIVAGLIISPILLGRL
                     GDTVFGIWQVLQRLIGHASPASGRPGEALKWTIAHEQRSTDYDFKRRQVGNALAVWLL
                     FLPLLLAIGGVLAWLSPVWLDVARDSYGTVRLAAGILVLDAVLFSLLYLPQSVLQGEN
                     LGYKRLGLSVLLVFVSKSLLVVAVYFGAGIGGLAVATAVATVLWGVTYLLIVRSRVEW
                     FGIARPSIAEVRHFVGLSWWFLLWNLVMTIMLASDVVVLGVVGSPALVTTYTLAKYLP
                     QAIIVAVATLIVAIMPGLGGMIGAGELARAVRIRRETMSLIWLFTIVAGTTVLLWEAS
                     FLQLWVGGEYYPGSGAVLVIMLMVLQFGLIRTDANIIDLTLNLRSKVLLGAISAVASI
                     GLAWLFVGPMELGIMGLVLGFIVGRTIQSIGYPWMIGRLLEIPPVRQLRGIVRPAIAT
                     AVLFGGAGVLADSLHAGSWVALVLSVGLSVAILAPVVFLAGLQPEVRRLLWLRLRRVA
                     RLT"
     gene            18282..19433
                     /locus_tag="VF195_01595"
     CDS             18282..19433
                     /locus_tag="VF195_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003868354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6579555.1"
                     /translation="MNVSADATAGGSTSSSTDARVRVAIFTANMDGGGAERAMAKLAA
                     GIADRGFDVDLVLSDASGHYLAEVPPEVRIVDLHARRVLASLPGLVRYLRRERPAAML
                     TSMNYVNVVGIWARALARVDTQLVVNEQNALSLEAAHSPRRRHRMLPRLIGRFYPWAD
                     RIVAVARGTADDLSRTAGLPRERIDVVHNPIVTPELKQLADAPLDHPWFRPGEPPVVV
                     SVGRLAEQKDFGTLLRAFAQVRDERDARLVILGEGPERGSLEALVAELGLDDAAALPG
                     WVQNPYPWMKGAGTYVLSSRWEGLPSVLIEALYCGVPIVAADCLSGPKEILEGGRHGL
                     LVKVGDVDGLARGIRSALGGEVPAPAPESWRPYEQEFVVSRYLEVLVGE"
     gene            19436..20641
                     /locus_tag="VF195_01600"
     CDS             19436..20641
                     /locus_tag="VF195_01600"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX6579556.1"
                     /translation="MRKIVFGLSLILIFTIPWEGAFHLPGLGTTATVMGLIVGACWLF
                     MIVVAGQMRRPVLFLFAVAGFVTWVALTTFWSPDPVESLGAVYLWLQTLLFVYILWDL
                     YRTKAALLAGLQAYVLGAYTAVGGAVLNYVQSNAFYTNEDRYSLGNTNPDGYGFILAL
                     GIPVACYLAASPETPRIFRFINYGYLPVAFIGVALSGTRTASVGAAIGLLYGLATLTK
                     LRIHTRVAVVLLLGASLFAVLPIVQPLSSFQRLGSTGTEITEGDLNGRTGQWAQGLRA
                     FMEHPVLGVGTDMYRTVNTLNKTAHNSYLSVLVEEGLIGFTIFLAILGIVALQVLRLP
                     KWDRNFWLTSLLVWAIGASTLTWEHRKTTWLFLTFAVAAAVIGARRSAPPDDVIDLTD
                     HDMAEVGVA"
     gene            20638..21525
                     /locus_tag="VF195_01605"
     CDS             20638..21525
                     /locus_tag="VF195_01605"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6579557.1"
                     /translation="MTLPTFLGIGVPRAGTTWLHQLLSAHPDVYLPSRRKEVRFFDRH
                     HEEGQGWYEGFFCPPDEAGRYSAIGEISPQYLYCDACPERISSLLPEAKLLVMLRHPV
                     DRAYSQFGFVIQRRDFRGSFEEFVASRPRALEMGFYSRYLERYLDHFDRQRILPILFE
                     EAVSEASTVRVELATFLGVAEGLFPDATERVNPSTVPRFRSISSAAVKVGRRMRRHNL
                     EWLVDVAGRMGLRRALTSGRRVPALDPQLRRELSGRYEREFEILERSWGLDLARWRAS
                     RATTPLGDGSAGVRQGGMS"
     gene            21522..22742
                     /locus_tag="VF195_01610"
     CDS             21522..22742
                     /locus_tag="VF195_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6579558.1"
                     /translation="MNRPLNVLLVSQPVSYGVAICVRDLTRAAVAAGHRVTVVSPDST
                     HGPLAGWIVAAGARHLPLNMARTPSLRDVSDLLAVRRLSRGRDVVHLHSSKAGALGRV
                     AAMSLGRRHRPAVVFTAHYWSWLVGGRWAGLYRWIERVLARRCDAIVAVSDGEAEEGR
                     AVLGARAPLRSIPNGVDRDRFSPIGDRADRTEDVPLIVCVGRLSEQKGQDVAIRALAL
                     LADRTVRLRLVGDESGHGERARLDALAASLGVSERIEWRGGVDATAPEYRAADVVVAP
                     SRWDGLSLALLEGMACGAAIVATEVNGSECLGEAGRIVSPDDPESLAQAVADLLGSAP
                     ERARLGRAARERSRSFDLATTMGRNLDLWSGLAIRAAGDPDAAATQAVAEDGAEDGAE
                     DGEGAARWTNTSTS"
     gene            22718..23692
                     /locus_tag="VF195_01615"
     CDS             22718..23692
                     /locus_tag="VF195_01615"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6579559.1"
                     /translation="MDQHLDVLMTERPALDAPIPYVCMPGSPYTGSTLLGMLLGNHPE
                     CASIGAATGLTARVDVSTYLCSCGARFTDCGFWGRIARRTVELGHPVTVFEKDFWNTH
                     VRVSRRRWLNGLLVSSLGSDVLTWCRDAVLHRAGPVARKIAEARSASWSLARAVLEQT
                     GKSTFVDTARDHQRPRYLADMPRLDVKVIHLVRDPRGNVASIMKHTKVSVEKAARQWR
                     HYNVEADRVRRRFPPESWMLLYYEELCAEPQASLDRVARFIGVQPAPITQLLENSERH
                     VIGNSMRLKVLDEIREDLSWQPTLTSVDLRTIARIAGPTSRRLGYEWP"
     gene            23689..24543
                     /locus_tag="VF195_01620"
     CDS             23689..24543
                     /locus_tag="VF195_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579560.1"
                     /translation="MSPTRPLLVFVHINKTAGTTLRYILRSSYGSRHCDVEPWHGAWV
                     DPPFSSEDLRRVRKLYPRLASIAGHRISGHVDLDEEGTDFRYLTFLREPIALCASRFQ
                     YQLDYRKKQDLVFEDWIEKAWARDAQTQRIGGTTDPSDAIRVIERKSMFVGLTERFDE
                     SIVLLQAQRAPDLNIAYAPVNVAKSSDIAKGLLADPATRQMIVDANAADLELYDHVVH
                     EIYPAQQRAYGASLSSTVATFRDGVQQRYDRRKLALYGLKQRAMLKPTLRLYRGRRTG
                     GVVRALIG"
     gene            complement(24548..25432)
                     /locus_tag="VF195_01625"
     CDS             complement(24548..25432)
                     /locus_tag="VF195_01625"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX6579561.1"
                     /translation="MLPTFVIIGAAKAGTTALYWYLADHPEVFMSPVKETNYFAFGLD
                     DDGHPLYGDPELHRFPIRSFAEYEALFAGSEGALAVGEASPIYLECPQSAARIREVVP
                     GARIVCFLREPVDRAYSDYLMYLRARGRSLDPDRDLTPDAPWARPESHWMQISRYHEA
                     LSRYYERFPREQIDVFLFDDLKNDAAACVRAVFAVLGVDPGFAPDLETPHNIGGLPSS
                     RTLERVLTSRSVRRVVEPWIPRRVSDAVRRLRTKNLKKAPPLPDQLRAELLRHFRDDI
                     GETAALIGRNLDHWLLPA"
     gene            complement(25442..26920)
                     /locus_tag="VF195_01630"
     CDS             complement(25442..26920)
                     /locus_tag="VF195_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579562.1"
                     /translation="MIVEFIGATGAGKSTLARELLRRGLESGSVRMATDLVIDRPGRR
                     WVENPHAVNLLADAAALPPFLRSFHDHRAFVRFAFRRLRHHAPSTFAKANYLRNVVRK
                     VGVHELTRWADPSVTFLNDEGTILIAYQLFVYSDAAPTPSEIERFSRLVPLPDLVVHV
                     KAPTDLLVERAMIRPDVRRELAGQDEERIGSWIRRAITVFDALAESEAIRDRVVTIEL
                     ADASPEGLDRAVGRLRDAVETRARALGSSTRSAAGGRSASRRATVIAFVGSEATGKST
                     VLDEVERWLSKRRRVRRVHAGKPPSTPLTSIPHALLPALRSLFPEQRTLRVEERYEGG
                     DATGTTYPLLFGIRSVMLAYERRALLRRAVRDGAGTVVLSDRYPSDASGAPDGPQLTH
                     LPMPKSAFSFRRILATVEGRLYRDIPTPDLVFHLTAPLEVTLARNAAREKKEPEDYVR
                     FRHELSSRLRFDGASVYPIDTDRDLDQVVRQIEVLIDAGREV"
     gene            complement(26917..27999)
                     /locus_tag="VF195_01635"
     CDS             complement(26917..27999)
                     /locus_tag="VF195_01635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6579563.1"
                     /translation="MLGETNGQYNEHCLPLMDERDISIVTYFTPKLTPPQEITLFAGD
                     GTLRGFFRALKAALRAKYDIIHAHAPQTGTLLVLAALAWGRFSRVRSSLVYTVQDSFH
                     DYKLRNQVMMIISVAAFRRIVFCSRSAYESLPRPLDRLVRRRSRVVPNGADLDRVDRA
                     IATRRPAPSRGGFTVISVGRLERVKDPLTLVNAFAAAVGDDAGSRAMLVGAGTLSTTI
                     EARARELRLQDRIVLTGLVPRDDVFVACADADVFVSTSHGEGLPVAVLEAMGAGCPVI
                     LSDIPPHRELADGADFIPFADPGDVDGFADAIRRFRAMTPIERREVGLRCREHMAARF
                     TLPIMRAGTEAVYREVAPLVGSGASA"
     gene            complement(28040..28774)
                     /locus_tag="VF195_01640"
     CDS             complement(28040..28774)
                     /locus_tag="VF195_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579564.1"
                     /translation="MTRIVETAVGRLGPTHRADRNIEILGVAGAGKSTLATHLAADPG
                     FEIAPFIHARRPADLLLILRTIPRLLPILWAGLTHAPRISWPEYKLLVYVTRWAPVLR
                     RRRPQTDTVLLLDQGPLYAIVRLSAEAKPFTERRSFTSWKREMLDRWSNELSEVIWLD
                     APDAVLWSRINVRDQDHRQKGGRTEAGHRFIARYRRAFESIIPVVEAWDDVRTTRFDT
                     STSTVAQIVEVVRASKRRREGNHPGG"
     gene            29088..30950
                     /locus_tag="VF195_01645"
     CDS             29088..30950
                     /locus_tag="VF195_01645"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX6579565.1"
                     /translation="MFVVSALSVIVIGTALPASANLPDTSTPSLELDRTITTQPFVGS
                     STTMRDGEGSAYVTPDGSLWLTEDHENLIFETNPHTGALKRVIDNAAFQNAPLFGGGP
                     VAGPNRTDDFESMAYDEANDWLFVFSGPCCDASHLPTVFRLVRVGGVLQVDSYQPLAA
                     TANNTASAWNPADQTLYVGQGQQIRTYDYVTNTTGTAFTVTGLSGILGFSFSSPADTG
                     GSDLFVVTNAERLRRVDWATKTLVPGWTFELTPFEVFDSRAVELIDEQFYVLDGYDSR
                     PPGPLRFAVFVFDVIGPQPVAPVASFTATPTNGSAPLSVDFDDTSTNNPTSWAWDFET
                     DGTVDSTAQNPTHVYQTPATYTATLTATNGAGSDTETAQITVTQAVAPVASFTATPST
                     GDAPLSVGFTDTSTGNPTVWAWDFESNGSVDSTSQNPTHVYTTPATYTATLTVTNGAG
                     SDTETAQITVNPPAPPTNLVGNPGFDVDTAGWSTAGSGTLVTLTRVTPGRDGTGGAAH
                     LVNGATGKRKCVLNDQPNWVTTTTAGTYTSSIWVRGDAAGALIKINIREMNGSTVVKS
                     RVGNFTLTTAWQQITVSHSILSPGSTLDLQVFLPKAQAPPGTCFYADDASIVRS"
     gene            31218..32516
                     /locus_tag="VF195_01650"
     CDS             31218..32516
                     /locus_tag="VF195_01650"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX6579566.1"
                     /translation="MDALTLVGVSAILGFPLVSIAYGVVTFLILNGDSSRKYRINPRL
                     GDEAGWLLGRVSVALIVLLSVGWVAQLAHIDLLGSGIRELTKVGLAVAAAVFLGRGAA
                     YALGRKAKERGLASEPTLIVGDGELAMGLAETFLEHPEYGLRPIGFLGRPLVRHVALP
                     LLGEVRNVERVIGDFEVRRVLVAFGEMRDQELVTILRASERVPAEIHVVPRFFELSGV
                     PQGAPVDDVAGIPLFHLRRPALRPGARFQKRAFDMAVASTLFLLSLPVFAVIALAVRL
                     SSEGPVLFRQKRVGLNGNLFEIIKFRTMYVNDESDTAWIAERDHERVTPIGHILRAAS
                     LDELPQLINVMRGEMSLVGPRPERPHYTGRFSETVSRYDDRHRVVGGITGWAQINGRS
                     RGLDSVPERARLDNYYIENWSLWRDLVIVARTVAVLFRGR"
     gene            complement(32524..33459)
                     /locus_tag="VF195_01655"
     CDS             complement(32524..33459)
                     /locus_tag="VF195_01655"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX6579567.1"
                     /translation="MITGAAEHLPEEGDPTIDEPATAPDTAPRFLATFDIEDWFHAEN
                     VRVSLPSIDPASMEPRLEPNAHALLDILADVGARSTMFVLGWVAERYPALVRRIIEDG
                     HEVASHTHLHRNLNQLTTDEMIRELADARDALEQVTGTPVRGVRAPNFSVSDHVLDVL
                     AEAGYWYDSSFFGFAQHDRYGTISATVDPDAAVVEVRPGLLEIQMTRLPVGPARVPWA
                     GGAYFRLIPYPVFRRGVAQRLRSTSWFTFYLHPWELDAEEQRLPQMARSLRLRAYTGR
                     GRMRRDLRRLIEEFGSARIDEALIARGHPPPGRTD"
BASE COUNT         5285 a        11190 c        11576 g         5707 t
ORIGIN      
        1 cgcgacgacg agcgtgtcgg ccggatagcg ctcctccgga gcgagcggga acccgtcgac
       61 ccccacgacc gtgaaatgga acccgtggag gtgcatcggg tgcagcagcg acccgtcgtt
      121 cgccagatgg acgaggacgt cgtccccgcg ctcggcgacg atcggcgtgg tggcggggaa
      181 cgacttcccg ttcaacgtgt aatcgagtgg gccatcaccc aggaatagat cggcctcgac
      241 gtcgggctcg accccgtaca ccgatctcca ccgccccccc ggcggttcga cgatgaacgc
      301 cccgtagagg cccttgccca cctgctcggc ggagttgaag tgcgaatgga agacgtacat
      361 cccgggtgga tccgtcacgc ggaactcgta atagaaggag tctcccggca tgatcgggtc
      421 ctgggtgacg tagggcacac cgtccatctc gttcgggatc gtcagcccat ggaagtgcag
      481 gacggtgggt tgatccatca ggttcttcag gaagatgcgc acgcggtcac cggttcgcac
      541 gcggatctcc ggtcccggga tctgccggtt gaaggccatc gcgtccttga agacgccctt
      601 cgagatctcc cactggaggt tctccgccca gagctcgaag accttgaccc caccgtcgat
      661 cttgggttgg agcggctggt tcccctgcac gtcggtctga tcgccggcga cgaaggcttt
      721 gacccccgcg gcgtgcccgt cggccatctg ctccgccgtc atcgtcgtgt gggctgccgg
      781 cgcgccgacg tcccccgcac cggccgtgag cgttcccacc atgcccgagt ccgcgtggcc
      841 cgggaccgtg cagagcgtcc cgtaggagcc ggcctcgagt gccgggacgc tgaggatcgc
      901 ggaccctccg ggatcgatcg tggaggtctc gatcgtctga tcgccgatcg tgaccccgaa
      961 cgtgtgcgcg gcctgcccgt ggttcatcac ggtgatctct agcggcgtcg cggcgggcac
     1021 gctgagtgcg gtcggctcga tcgcgaactc cgacagcgtc acctccaccc ccgcaggacc
     1081 ggctgcggcc tgggtgccgc cgccggtgct cggcgatccg gtgggttcag ccgtgcagcc
     1141 agcgaggacg agagcgagga tcgtgagtcc gaacaggttg aacttcgaga tggacgtgcg
     1201 catcgcagct ccttcggctc gagcgaggga tgctcatcgt cgttcgtggc accgggttcg
     1261 ccccgggtcg aaaggcccgt cacggagagg accagcggcc ctccgcgctc ggttccgata
     1321 cgtggtcggc gctcagccac gggggagcgg cgccaccgag tcgcgacccg gtacgggatc
     1381 gccgggaccg ccccaggatc gggcgccagg aacagtgctg cgcgcagcgg gccgacccta
     1441 ggaaccggtg tctgcctcgt ccgagtgggc gagaggcgct tcaccgccgc tcaggacgag
     1501 ggccaccgct cgacctgccg gtgctgaacc tcgccctcat ccactgaccg ccgcatgtcc
     1561 cgcggaccca gggtccgatg acccatggag gccttcacga tgccgagcat gctctcgagg
     1621 tgacggcgga ggcgatcgta tgtggcagcg aggggccagc agagtaggtc tcgcgatcac
     1681 gtgcaccgtg gcactggcgc ggccggcgat ggcgaccgac ggagacctcg atccatcgtt
     1741 cggtgaggac gggcgcgtcg cgaccgtctt cccggttggc tcgttcgcga ccgcggtcgc
     1801 gatccaggac gacggcaaga tcgtggcggt gggcgcggcc gcggggccgt cggaaaccgg
     1861 cgagttcgcg cttgcgcggt acgaggagga cggcggcctc gatcccagct tcggcgaagg
     1921 gggcatggtc accacggcga tcgccggagg cggcggcgac gaggcccgat cggtcgcgat
     1981 ccaggagaac gggcgcatcg tggtcgccgg tgccgacagc tggcagcggt tcgccgtcgt
     2041 ccggtatcgc cccaacggcc agctcgacga ctcgttcggg ggtggtgacg gcatcgtccg
     2101 aacgaacttc acaccggggg acgacatggc gtgggacgtc gtgctccaac gtgacgggaa
     2161 gatcgtcgcc gttggcgggg ccggactcgg gcatgaggga ttccagatcg cgaggtacca
     2221 gcgtgatggg acgctggatc ccacgttcgg tgagggtggc aaggtagtga cgaggtacct
     2281 ggacgccaac gcgcgggcgg tggcgctgca accgaacgga cgcatcctgg tcaccggcac
     2341 caacgtgcgt tggctcgcgc tcgcacgcta ccgcccggac gggcggctcg accgctcctt
     2401 cagcggcaac ggcatggttg gcgtcgcgct cgggatctac atcctccctc acgccgtggc
     2461 attgcagcga gacggccgga tcgtcgtggc gggcggattc gactacgacc acatcggcct
     2521 ggcacggttc cttgacgacg gccgactcga ccgctccttc ggcggcgacg ggatcgtacg
     2581 aaggaaggta gaaggcgagg cacagcaggc gatcggtctc atcctgcaac ccgacggccg
     2641 gatcgtcgtg gccggctggt caggcccaca cgaggctgaa ccgaccgtct accgcttcat
     2701 catgatccgc gtcctcccgg atggacgctt ggaccagacg ttcggggatc gagggagggt
     2761 cgccaccttc ttcgatggtg gagcaatcgc gtacggcgcg gcggctgatc acgaagggcg
     2821 cgtcgtggtc gtcggcggag caggcgaggg gaaccgcgat gcgttcgcgc tagctcgcta
     2881 cgtcgtctga gtcgcggcgg cgtcgcgatc catccgagag ggcgggcgag catcgtcgat
     2941 gtgcgcgctt ccttctcgat gggctgttca tccgtaggtt gcgggctgag tcccaccccc
     3001 ggagctcggt tgacccgcga cgatgttttc cgattccagg cggtatcggc cgatccttcg
     3061 cctaggagcg tgaaagggca ggggggccac atctgcttca cgacctcttc ttcgccgatc
     3121 cgcgcagctg ttgaccctag gagtcggtgt cgtcctcgtc cgagtgggcg aggggcgctt
     3181 caccgccgcg cacgacgccg gccagtcgct cgacctgctc ggcgacctga tgcaccatcc
     3241 cggcgaggac gcggatgtcg tccgcctcgc cgggtgggcc ttcgccgttc gccatccgct
     3301 ccgccgtctc cttcgtcttc cgcgcctccg cgatgatcgc gtcggcgtcg tccgccatca
     3361 gttcttccgc catgatcgag ggtcctttcg gtcggccggt gttgacacct tgcccccgca
     3421 cggcgcggga caaaccgcct tgagaagtcc ggcgttcaga gagtgtccga gttcccggct
     3481 gctcgtgccg ggccaggatc tcgcggagct catcgagcgg cagcgggtgg ttcctggcaa
     3541 ggatgcaggc aaagatggag gagcgcgacg agggaggcgc taggaatcgt cgagagcgga
     3601 gggaccgacc aggggttggc accgcgggca ggcgaaggtg atccgctccc gggaccgagg
     3661 tcctcgggcg aaggaacgga tcggcgtgcc gcagcgacga cagggtctcc cgccccggcc
     3721 gtagacccag agatcgtggc cgggccgcgg gtcgtccgtc gtgacgcgcc ggccgccggc
     3781 gcggttccga tccatcaggg agcgcatcag ctcgacgatc ccgcccgggt ccgcgagcga
     3841 gccgaccgcg gtccgcgggt ccaccccggc caggaaacac acctccgagc ggtagacgtt
     3901 ccccggcccg gccatcaccg actgatcgac gagcgcgtcg cccacctccc ggtccgggga
     3961 cgcgacgagc cgacgggtcg cctcggcggc gtcccagtcg ggtccgagga cgtccgggcc
     4021 cagatgggcc aggagctctg gctcaaccct ggtccggatc acctcgagcc gtcgcagccg
     4081 gtggccgacc gcgatcctct cctcggtcgt gagcacggcc cgaacctcga acccccgacc
     4141 tctccaccgc tcgcccggct cgtacagccg ccacgacccg tccatcccga gatgcgagtg
     4201 tacggagacg ccgccatccg ttcggacgaa gagatgcttg cctcgcgcgc ccacctctcg
     4261 gacgctctgc cccgacaggt cgccggccgc gtaccgcggc acgcggaggt cggtcgcgat
     4321 gagcacgtgc ccggcgagcg cgtggtgcat ccgcgccgcc gtcgcgaata ccgtgtcgcc
     4381 ctcagccatc ccgacgccac tcgaagcgag ggcgctggca tcatcatcgc tgatggccgc
     4441 ccgatcgcct cgagttctct gtccgttcac ctcctcgccc tcggtcgtcc ctcctggtgc
     4501 ggtaatcctt tactcgatgg cgagccgagt gcgcgaaagg atgggatcga tgcctggcgg
     4561 gatcgacgat gctcgtggca cggacgctga tgctggagag tacccgtcat cgttcttccg
     4621 cggtctcgcg gtggcgatcg tcgccagcgc cccgctgtgg ttgttgtgat ccgggcggtt
     4681 cgctagctca tccggaacac gtagaccgcg tacttgcgcg gatcggtatc cgggcgagcg
     4741 ggatcaccat cgaggacgta gtaccggcga tcgatgtact cgacggcacg agcgtcaccc
     4801 accccgaagg aaccgagctc gagcacccac tcggcggcga gcctcttcct cggccagacc
     4861 acccggtgga gctcctccgc ggtcgtcgtg acgaagagac tgccgccgtt cgggctgaag
     4921 ctcataccgg tgatccccga gaggttgggg acgttgaaga ggcgacccgt gtcgttcgcc
     4981 gggtaacggt aggagcgaag atccctccct tgccccacgt agatcatgtg attggcggcg
     5041 ttccacgcgg ccgccgtgaa gttcgcggat cttggcaggg gctggaagga ctcgacgcgg
     5101 aaacgcccgg ctccggtgcg cttgagccgg aatgccgtgg gtttcaccga cgacgagcag
     5161 cacggcccgg agaagacgta gagagcgtca cgggcacggt cgtatgcgat gctctccaga
     5221 tcccgtgtgc ggtcgatccc tgccctgacg ccgccgccga gccgccgtgc cgatgcgagc
     5281 ttcttctggc cgatcacacg tttcagggcg ccagtggagg ggttcacctc gtagatgcgc
     5341 cgtccgtagt catccgccag ccagagggag tgatcgcgcg gtacataggc acttccctcc
     5401 gcgtccttca tggaaacggc cttgttcacg aacggtgtcg ttcggatcag tctgacgaga
     5461 tgccactgcg gagcggccac gaccgaaccg aacgtgacga tctcgtacac cagggcggtc
     5521 gcgaccacga agcagaggga gaggcacgcg tgcctgccac gttgacttct gaccaccgtg
     5581 agcaacacct cggatgacat ccggccatgg ggtccgcgct gccattcccc gatctggggg
     5641 gttccaaacg cggccatgac gccgagacga cgatcactgc ttgctatggt cccaagatgc
     5701 gggagcgtac gatcatcgta accggcgggg ccgggttcgt cggttcccat ctgtgcgacc
     5761 gtctcctggc cgagggtcac caggtcgtgg cggtggacga tctggtgacg ggtcggacgg
     5821 agaacctctc gctcgcttcc ggccacgggg atgccttcgg attctcggag accgatatcc
     5881 gaacaccggg cctgcaagac gtgttcgatc gccatcgccc agacgtggtg atgcacctgg
     5941 ccgcgcaggc cggcgtccgg ccgtcgctcg aggacccgga tcacgatgcg agcgtcaatg
     6001 tcctcggaac cctcaacgtg ctccgatgcg ccacgtcggt cggttgtcgg atggtggtgt
     6061 acgcggcttc cgggggaacg ctctacggag aaccgacatc cctcccggta cgggaaagct
     6121 ccgccgacgg tgcgcatccg gaatcgccgt acgggatcag caagaaggtc gttctcgact
     6181 acctccgctt cttctctcgc catcggagtc tggagttcgt ggcgttggcc ctcggcaacg
     6241 tctacgggcc gcgtcaggat cccaccggcg aggcaggcgt gatctcgatc ttcgcatcgt
     6301 ccatgctggc ggggcgctcg ccgacgatct tcggtgacgg cgaacagacc cgcgactacg
     6361 tctacgtgga ggacacggtg gccgccttcg tcgccgcgat ggatcgagct tcgggcgaga
     6421 tcgtcaacat cggcaccggc atcgagacgt cggtcaacgc gttgtactcg atgctctgtg
     6481 agatcgtcgg catcccagcg gccccgaggt acggacccca gccggaaggg gagctccgtc
     6541 gcatcgctct cgacaactcg catgccgctt ccgtactcgg ctggcgtccg cggaccgatc
     6601 tcgccgtcgg gctggcacgg acggtcgagc acctcaggga gtcctactcc ccgtcggccg
     6661 atgcgaggtc tgactcggac acggagccct gagctgcact cacgatgtca tgcagctcgg
     6721 ccggcgttgt gcccgcggcc gaggcgagca cctcgacgtc gccggctcgg agcgcgatcg
     6781 tctcgacatc cgggtcggct cgctgcacga gcacctgctt gatgaagccg gcgatcagct
     6841 cgccctccgg ttcgtggagc gcctcgagct tcgtgaggtc gatcctcggc tctccgtgcc
     6901 cgtagatggc ccgggtgatc tcctggagga ggcgtgccgg tgacacatcc agcaagcgac
     6961 agagttcgca gaagcgaacc acggagatcg ttcgctcacc tcgctcgtat cccgccaggg
     7021 acgtcggccg gaatcgcccg gcagatgaat cagcgagccc tctgagggtc atccctcgat
     7081 cgagccgcgc gcgacggagc gcacgtccga tctccagggc cagctgctcc atcgttcctc
     7141 ttcctcatct ggccatgggg gtggcgttcg agggaaacat agtgcctgat atggggtcgg
     7201 atcggtaggc gcgggggccg tagatttcct gccatgcctg ggtgatgccc cgtacgtatc
     7261 ctgtggaacc cgctccgcgc tctcgcccac tcgcctgccc ccgggactgt tgacggaggg
     7321 gctcgagacg cacacgatat ctgatccgca ccggttgagg agaactcgct ccgggttgta
     7381 cggtacctcc cccgtaagcc gattggtcta cgctgaacag atgaaagaca ctggcattcc
     7441 tccccctttt gggggacaat cttcacacgg agcgattagc ttgtcaggat gagggaaagt
     7501 agaacaggct gtggccttga ccgatcggct ctgcgcgacc ggtcggctct tcgggaagag
     7561 attggggagt gtgatggggg gagttgatcg atctcgtgat ggacgcgtgt cgagtgtgat
     7621 cgagagccct tcctacgcgc tcgcatcggc gagtcgtccc gaggaagccg ccgcgcacgc
     7681 gacatcgtgg gtccgcgaga tccttcatgg agatgtttcc gtgcatatcg ctcgactcga
     7741 cgcggcaggc cggctccgca cggtgtggcg cgacggagag aacagctcta ccggcagcgg
     7801 gctcacgact gcccgccgta tcgctctgca gacgcgagcg tactcacatt tcgacttccc
     7861 gcggatcacg ggcgcgcggc gcgcggcgct cttcccgctc gtctgccgcg agcagtcctt
     7921 ggggctcctc gagatccagg cggatcgagc cgccctcgaa gaacgttccg gcatcctggc
     7981 agccatcgcc tgtcagctcg cgggtgtctt ggccggcctc gacgagcgag atggtttggc
     8041 gcggcaggta ggcaccctgg aggagctcgc cgacatcgcc cgtatcctcc tccgtgccag
     8101 tggacccgag gaagcgctcg cgatcgttgc gagcgctctc tcccgtgatg tgcgggcccc
     8161 ggtcgcgatc tggtggggtc ccgatgacgg agtgcggacg ctcgcctcgc tttcgggtgt
     8221 gaacgctgcc aagacggaag tcgtcatccg agaccttggg gaggtgccac cgtcggatca
     8281 gttgtccgat ggagagcgcg ccgaactccg tcaacggttc gcgcgtgtgc tcggcgtacg
     8341 aggcgcgacg atccgtgacg cggccggtgc gctcgtgctc gtggcgagtc gcagcccgtt
     8401 cgcgcagcat cgggtcgacg tgatcggctc gctgctggag gacaccttgc ccttgttgtt
     8461 ggcgtccgcg cgtgaagcga agcgatcttc gtacctcgat gtcggactcg catggacggc
     8521 ccatgagctc aggacaccga tcctcggcgt caaggccgca ctggaaggcg ttgtcgggcg
     8581 gagcggtccg cgggatgagt ccgtgctccg acgctcggtg agcgaactcg agaaactcgc
     8641 gctggacacc gaggggatcc tcgggtgggc aaccggtcgc cgcgagctgg atacgtccgt
     8701 cgtcgatgtc tcccgactgg tggaggacgc gatcgccagc tatcgggttc tgcatgatgt
     8761 ggaagcaacc atcgtggtcg atgcagatcc gtccgtcacg gcttccgtcg atcccgtgca
     8821 gttgcgtgca gccgtcatga atctcatccg gaacgcggcc ctctattcgg gtcgcgacgc
     8881 gatcgtgcgt gtgcacgtcg cgagcgaggg cacggccgtc cgggtgtccg ttcgcgatca
     8941 ggggcccggc atcccgcagg aggagcgtgc cgccatcttc gatccgttcg tccgcgggcg
     9001 ggagggatcg agggttcggg atggaagcgg tctcggactg ttcatcacac gtcgcgtggt
     9061 cgaagcgcac gggggtcgga tctgggtcga tcccgacgac gagggctcca cgttccacgt
     9121 gttgctgccg gtggatggga gggaactgca acggtccgca tcctgatcgt cgaggatcac
     9181 gtcctgttcg ccgaagcgat cagctccacc ctgctcgagg cggggatgga gccggtgggc
     9241 ctggccacga gcggcagggg cgccctcgag cacctcgaac gtgacaggcc cgacgtgatc
     9301 ttgctcgacc tcggcctccc cgatcgcagc gggctcgcgg tcggacggga gatcctggag
     9361 gactggccgg aggcgaaggt cgtggccctg accgccctcg acgatcagaa gacggtcgag
     9421 gcagcacagg tcgcagggtt ctgcggatac ttgtcgaaga acgtgccggt cagccgattc
     9481 gtcgcctcga tcgaggccgt ggcggccgga cagacggtct tcccgcatcg cgcggcatct
     9541 cgacgcacgc acagccctac gaacgacagc gtatggctgc tcgtgtcgca gctgaccgcc
     9601 cgagagcgag aggtgctcga gctcctcgtc gaaggcgcgg gcggagcgat gatcgcgcgc
     9661 cgcctcggca tcagccggaa cacggttcgc acgcacgtcc agagcatcct tacgaagctc
     9721 caggtgcatt cacgcctcga ggcggccacg ttggccgtgc gacatcgtgt ggtccccgtc
     9781 ccctccaacg tggacgtgga cgtgtcgact cccttcgaca gggtctgaaa cacccgtgac
     9841 gcaggtcgtc tcctcctcgg ctcatgcaga tgacgtagcg cggctacgtc gtccggtgcg
     9901 tgcccgggaa gcgatctcgc gggtacggtg ctcgcgtcac gtctgaccga agagagagtg
     9961 tgggttgagc gagccttccg ggacgatccg cgtgctgttg gcggaccggc acgccctctt
    10021 ccgtgaggcc gtccgggcgg tcctcgaaag cgaggccgag atccatgtca tcggtgaggc
    10081 cagcaccgga cccgaggccg tcgcggaatc ggagcggacg ctcccgcacg tcgcgatcct
    10141 cgatgcggac ctcccgatgt ccaacagccc ccagaccacg gcagcgatca aggcgaacgc
    10201 tccggactgt cacgtcctgg tgttgagcgc ccgggaggac taccggggtc tcgttgaggt
    10261 cctggatgcg ggcgcgagcg gatatctcac tagggaatct cctctcgcag acctgatcca
    10321 tgcgacccgg gcgatccatc ggggcgacac gctcgtcccc ccgacgatgc tcggcccgct
    10381 gctcacagga ctcctccgtc ggaagcgtga ccacgacgaa gcgttcgagc ggatcacccg
    10441 attgaccgtg cgagaacggg aggtgctggc gctcctcgcc caagggtcgg acaacgattc
    10501 gatcgcccga gctttggtga tcagtccgca gacggcccgg acgcacatcc agaacatcct
    10561 ggggaagctg ggggtgcact cgagactcga agccgccgcg ttcgtcaccc gcagcgggat
    10621 gctgcagaac ctcgtccgag cggagggtgc gaacgcctga aggtcaggct ggggagggga
    10681 gggtctcgcc ctcccccgac cgatccaacg gcgcatcccg gcggatccat ccgatcgtct
    10741 tggagaggcc ctcgcggagg gggatctgcg ggctccaccc gaggcgatcg ttcgcgaggg
    10801 atgtatcagg acgacgattc tcggggtcgt ccacgggacg ttccgtgtag acgatcggag
    10861 accgggcgcc cgtgagcgaa cggatcaatt cggcgagctc cgccatcgtc acctcttcgg
    10921 tgctgccgag attcataggg ccggtgaggt cggagtcgag gaagcgccag atgccttcga
    10981 cgagatcgtc gacgtaacag agcgaacggg tctgggagcc gtctccatgg acggtgatcg
    11041 gttcagcgcg gagcgcctgg tcgatgaacg tcggaacggc tcggccgtcg tgccgtcgca
    11101 tcctcggccc gtaggtgttg aagatcctga tgatccgcac cggcacaccc cgcgtcctgt
    11161 ggaaggccat cgtcgcggcc tcggcgaagc gcttcgcttc gtcgtagacc gatcggggcc
    11221 cgatgggatt cacgttcccc cagtaggtct ccggctgcgg gtggacctgg ggatcgccgt
    11281 acacctccga cgtggatgcg agcaagaggc tcgcgccctt ccgctccgcg agacggaggc
    11341 atgccaggga gccgagggcc cccacctcca gggtctcgat cgggtgcgcg aggtagtcga
    11401 cgggcgatgc cggggacgcg agattgaaga tccagtcgac cggaccctcg atgtcgaggc
    11461 ccttggtgat gtcggcttcc tggatcgaga agcggccctc ggtcgccgca tcgctgaggt
    11521 tctcccgtgc tcccgtgagg agggaatcga cgcagacgac ctcccaacct tcgtcgagga
    11581 gtcggtcgca gagatgcgag ccgacgaagc cggccccgcc cgcgacgacg gcccgaggag
    11641 cggtcacgtc cttcggtgct cggccgggcc ctgctcggcc gggcgctgct cgaccggggg
    11701 ctgctcgacc cgggcgggcg gagcagtctc gggcgaggtg agcgacgcga tcgtcgtcgg
    11761 ttcgacggcc ggacggcctg tcgggtaata ccagaagccg gcttcggcca tctcgtcgga
    11821 gtccaacagg ttcctcccgt cgacgacgag cgggtaggtc atcacgtcac ggagcttggg
    11881 gaggtccagc tcgcggaatt cctcccaatc ggtggcgacc acgatggcgt gggctcccga
    11941 cgcggctccg tacacgtcgt tcgcgagctc gagctcgggg agctcctcct tggcgctggc
    12001 gccggcgtgc gggtcatagc cgacgaccag ggcccccgac tccaacaggc gacgggcgag
    12061 cgcgatggac ggggagaagc ggacgtcgtc ggtccccggc ttgaaagcga ggcccagaac
    12121 gacgatccgc ttgccctcca tgttccagag ggcgtcctcg accttcgcgg cgacggcctc
    12181 cacggcctcg tcgttcaccc gctcgacctc ggccaggagc gggaacgggt accccagtcg
    12241 ctcagagagt cgccgcaacg cagccacgtc cttcgggaag cagtacccgc cgtacccgag
    12301 gcccgcgttg aggaacgcac gtccgatacg tggatcggaa cccatcacgt ccgccacggc
    12361 ggtgacgtcg gcgccggccc gctcacagat gcgcgccatc gcgttcgcga acgagatctt
    12421 gagggagagg aacgcgttgc tggcatgctt cgcgagctcc gccgtcggga tgtcggtcac
    12481 gatcagttcg acgccatcgt cgaggagggg cgcgtagagg cgccgcagga cctccagcga
    12541 acgaggggac tcgacgccga ctacgatccg atcgggcttg agcgcgtcct ccatcgccga
    12601 accctcccgg aggaactccg ggtttgaggc gacgtggaac cggcgtcccg cgccctcccg
    12661 ttccaacgtc gttcggacac gatcggccgt gcccgccgga accgtggact tctcgaccag
    12721 caccgtgtcg tcgggtgcgt gctgggcgat caccttggcc gactcctcca tcgcgagcag
    12781 attggcgtct ccgctcgctc gtgccggtgt gccaacgcag atgaagacga cgtcggtgcc
    12841 ccggagggcc tcggctgcgt tcgcagtgaa ggaaagtcga cccgacaccg actcccgtgc
    12901 catcccttct tcgagcccgg gctcgaagaa gggagggata ccacggcgca ggagctggat
    12961 cttctcgacg tcgatgtccg tgccgacgac ttcgtggccg atgctggcga acatcacgct
    13021 cgtcaccaga ccgacgtggc cggtccccat gaccgtcacc ttcatcgcgc gctcgatcct
    13081 cgactcatca tgcgagggta ggtcctctcc gtcccgtagc cgaccggcgt ggtccgtagc
    13141 ttccgtacgg agctcaccgt accccgcgta gatgaagtca tgcgcgggac gaggtcttcg
    13201 acgacctgga agcctcctcg cgaagtactg cgagctctcg gtccgcatcg tcgtaccggt
    13261 tcacggttcg gcggccgttg taggagccgt atttcggctg ggtcatggat tccgggatgc
    13321 ggttgaggat gccaccgagg atgtttcccc cgacctgcgc cagctgatgc cgtgcctccg
    13381 cgacccgtgc gcgggtgccc tcctgggcgt ccgcgaccac gatgacggca tcgaggagat
    13441 cgacgagcat gagactgtcc gcgaccggtc cgatcggggg agcgtccacg atcaccacgt
    13501 ctgcctggcg acacccgcgg atcacgtcga ccatccgatc ggactgcagg agctcgaccg
    13561 gctcgtcgtc ggcggtcacc ggtcccgtgg ggagcacctt cagattctgg atcggcgtct
    13621 cgacgagtgc atcgcggagc tcgatcgatc cctggagcac ctgaccgagg ccctgttcgt
    13681 tcccgatccc gagaaggacg tggacacgtg ggaaccgaag gtccgcggag atgagcacga
    13741 ccgacttccc cacctgcgcg agcgcgaccg cgaggttgac cgcggtcgtc gtcttcccct
    13801 cgccttccag ggcgctcgta acgaggatcg ttcgtgcctt ggcgtcccga gctgccgcga
    13861 ggacgttcgc tcggagtgct cgatacgctt cggcgggaga acttcgtcgc tcgtcgatcg
    13921 ttaccagacg cggcagatct cccctgagga gctgagaatc cgggatggca ccgagggtcg
    13981 gcgcccgcag gacgttccgc agcgtggcgg gagtctcgat ccgatcctgc atccgctcgc
    14041 ggacggaagc gagggccatc ccggccgcga tccccaggag aacgcccaag gcgagatcca
    14101 cctgatgctt cgggctgatc gccgaggacg gacgctccgc ggcgatcagc acctcaccgg
    14161 gatccctgct cgagatgatc gtcgtcacga gttggttccg caagccgagc ctcgtgtctt
    14221 ccagggagac ccggtcggac tcggccgccg ccagctcggg cgagccctcc tccatcccct
    14281 gcatgtcgat ctccagctgt cgcatcgcca gatcgagctg gtcgatctcg gcctggatcc
    14341 tggcggcatc ctcatcgatc ccatcccgcg cgagctcgcc cttgaactgg aggtagccgt
    14401 ctgcgaacgc ctgcgcgccg ctctgcgctc gcttcggcgt ggagtccgtg taggagatct
    14461 ggaggatctc cgtgttctcc gggatcgtca cggtgacccg ccggagcatg tcctgcagat
    14521 cctgcggtcg cccgatgagc gttcgtgcga tgtccgcaac cggtgtggac cggaccatct
    14581 gggcctcggt gagcatgttc aggttgtccg aggggtcctc atcggtgggc tgcacgcgga
    14641 tcggcctggc cacgacgctc gccgtcgacg agaagacctt cggacgagag tacgagtagg
    14701 ccgccgccag agccacgacg acgatggcga cccccagcac gagccacttg cgccggagga
    14761 ccgttcccat gaactcccga aggtccagcg atccgtcgcc cgatgcgggc gccacggagg
    14821 gaggttcctg gaacatcctg ctcatccgat ccctgtctcc tcgatccgat cccacgtagg
    14881 aaccggcgcg cggcgtctac ggccggccgg tccgataccc acggtagaac ccggtgcgcg
    14941 tagccaccat cgtgcgaaca tcgtagtgcg gctacgtcat gatgccggtg ctgcgcgagc
    15001 catactcgcg taccgttgtt tcgtcatccc gaccgcggtg gagcaaccat gtctgacaca
    15061 gtcctcagat gagtgcgagt agcagtgctc acctgcgcgg atccgccgat ccggttgccg
    15121 cgagcggcga ccgagacggg ctcccatccg atgtcgtgac ggccgcctcg ctcgaagacg
    15181 gttccgttgc ggtggccgat ggcgttcttg tgttgatcgc ggccctgtgc cgttccctcg
    15241 aggccgagcg tgttcgctat tgtcattgga agagcaacga atcgatcgcg cgctccgcct
    15301 cgggcgagaa cgacctcgat ctgctcgtcg cacgagccga cgcggaacgg ttcgagcgga
    15361 tcctcaggcg cttgggcttc aaggacacgg accaaccgtc atggaagcgg ctgcccggca
    15421 tctaccactc ttacgggctc gatccgacgg gcgtgttcgt tcacatccac gcgcactacc
    15481 agctggtgat cggcgacgac atgaccaaga acatccacct gccgatcgaa gcgccgtaca
    15541 tcggatcggc tcgacgggaa gggatattcc cggtcccctc gacggccttc gagctcctgc
    15601 tgttcttgat ccggatggtc gtgaagcact ccacctggga cgcgttcgtc tcgttccagg
    15661 ggtcgctgtc gaagagcgag cgccgggagc tggaggatct cgtcgagagg acggatctgc
    15721 acgaggtgtg ggagctcgcg gaacggcatc tcccgttcat cccgggcgag ctcctggaga
    15781 ggtgcctgcg ctgcgtgcag ccggacgcgt cgcgatggct ccagatccgg acggccgccc
    15841 gcctgcaacg cgtgctcgcc ggctgcagcc gacggccacc ggtcctcgat accggactgc
    15901 ggatgtggag acgtggcagg acggtgctcc ggcgcaaggt cgtccgtcgg gggcccgtca
    15961 ggagccggat gtcctccggc ggcgcgttgg tcgccatcgt gggaggagac ggggcaggga
    16021 agacgaccgt ggtggacggg ctgtcgagct ggctcgcctc cgagcacatc gactccacca
    16081 cgatgcacat ggggaaaccg tcatggtcgg cgctcacggc gatcgtcaaa ggctcgctga
    16141 agctcgccgc ggcgacgaaa cggacctcga cttcgtccgc gaaggcgttg cggtcttccc
    16201 tttccgccgg cgagggcggg ttgagcgtgc ggaaccgagc gcgcctcgtc tgggaggtcc
    16261 tcacggcccg cgatcgcttc cgcacctacc ggaaggcgag acgtctcgcc acgaacgggg
    16321 cgatcgtgat ctgcgaccga tacccactgc cagaggtctc tctcatggac ggagcggtga
    16381 cgggccggtt gaaggatccg gcggcatggg gccgatgggc cgcatggctg gccggactcg
    16441 agcgacgcta ctacgagcgt atccgggacc ccgacacctt gatcgtgctg cgggtcgacc
    16501 ccgatacggc cgtggagcga cggcgcggga tcgaaccgga gtcctcggtt cgcccgcgct
    16561 ccgaggagat ctgggggatc gactggagcg gaacgtctgc gatcgtgatc gacgcggctt
    16621 cgccgaagga cgacgtcctg tccgaggtca aggccaccat ctggtccaga ctctagtgac
    16681 gccgaaggac ggcggatgaa gacggatgac acgactccga ggtccgcgcc gagaggacgg
    16741 ctgggccgga tggtctctcg ggtggtctcc gacggcagcc tgacgaagaa ggcatccctc
    16801 aacgccgccg cgtcgacggc ggagcagggt gcgaggatcg tcgccggtct gatcatcagc
    16861 ccgatcctcc tcgggcgact cggggacacg gtgttcggca tctggcaggt cctgcagcgc
    16921 ctcatcggcc acgcgtcacc ggcgagcggt cgccccggag aggccctgaa gtggacgatc
    16981 gcccacgagc aacgttcgac cgactacgac ttcaagagac ggcaggtcgg caacgccctc
    17041 gcggtgtggc tgcttttcct tccgctgctc ctcgcgatcg gcggcgtctt ggcctggctc
    17101 tcgcccgtgt ggctcgatgt ggcgcgggac tcctacggaa ccgtccgtct ggccgcgggt
    17161 atcctcgttc tcgacgcggt cctcttcagc ttgctctacc ttccgcagtc cgtcctccag
    17221 ggggagaacc tcgggtacaa gcgactgggg ttgtcggtcc tcctcgtctt cgtcagcaag
    17281 tcgctcctcg tggtcgccgt ctacttcgga gcggggatcg gcggattggc ggtcgcgacg
    17341 gcggtcgcga cggtcctgtg gggcgtgacc tatctgctca tcgtccggag ccgcgtcgag
    17401 tggttcggca tcgcgcggcc gagcatcgcg gaggtccgtc atttcgtcgg tctgagctgg
    17461 tggttcctcc tatggaacct cgtgatgacg atcatgctcg cgagcgacgt ggtcgtcctc
    17521 ggcgttgtcg gttcgcccgc gctcgtcacg acgtacacgc tcgcgaagta ccttccgcaa
    17581 gcgatcatcg tcgcggtggc gacgctcatc gtcgcgatca tgcccgggct cggagggatg
    17641 atcggcgccg gggagcttgc gcgcgccgtc cgcatccgca gggaaacgat gagcctgatc
    17701 tggctcttca cgatcgtcgc ggggacgacg gtgctcctgt gggaggcgtc cttcctccag
    17761 ctctgggtcg gcggagagta ttacccgggg tccggcgccg tcttggtgat catgctgatg
    17821 gtgctccagt tcggattgat ccgcacggac gccaacatca tcgatctgac gctgaacctc
    17881 cggtcgaagg tgctcctcgg ggcgatctcc gccgtggcgt cgatcggtct cgcatggctc
    17941 ttcgtcggcc cgatggaact gggcatcatg gggctcgtcc tcggattcat cgtgggtcga
    18001 acgatccaga gcatcggcta cccctggatg atcggcaggt tgctcgagat ccctcccgta
    18061 cggcagttga gagggatcgt ccgtcccgcg atcgccaccg ctgtcctgtt cggcggagcc
    18121 ggtgtgctcg cggattcgct ccacgccgga tcatgggtgg cgctcgtcct gtccgtcggc
    18181 ctttcggtcg cgattctcgc gcccgtggtg ttcctcgccg gcctgcagcc cgaggtccgg
    18241 cgcctgctct ggctccgact ccgtagggtg gctcgactca catgaacgtt tccgcggacg
    18301 cgacagccgg cggctccacg agctcctcga cggatgccag ggtgcgcgtc gcgatcttca
    18361 cggcgaacat ggatggcggg ggcgccgagc gcgcgatggc caagctcgcc gccggcatcg
    18421 cggaccgcgg attcgacgtc gacctcgtgc tcagcgatgc ttcggggcac tatctcgcgg
    18481 aggtcccgcc ggaggtccgc atcgtggacc ttcacgcccg ccgcgtgctc gcgagccttc
    18541 cgggcctcgt ccgctatctc cgccgggaac ggcccgcggc gatgttgaca tcgatgaact
    18601 acgtgaacgt cgtcggtatc tgggccagag ctctggcccg ggtcgatacg cagctcgtcg
    18661 tgaacgaaca gaacgccctc tcgctcgagg cggcgcactc gcctcgacga cggcatcgga
    18721 tgcttccgcg gttgatcggc cggttctatc cgtgggcaga tcggatcgtg gccgtcgccc
    18781 gcggcaccgc cgacgatctc tcccgaacgg cgggccttcc ccgcgagcgg atcgacgtcg
    18841 tgcacaaccc gatcgtgacg ccggagctga aacagctcgc ggacgctccg ctcgaccacc
    18901 cgtggttccg ccccggtgag ccccccgtgg tggtctcggt ggggcggctc gcggagcaga
    18961 aggacttcgg aacgctcctc cgcgccttcg cgcaggttcg cgatgaacgt gacgcccgct
    19021 tggtgatcct gggcgagggg ccggaacgcg ggtcgctcga agcgctggtc gccgagctcg
    19081 gtctcgacga cgcggcggcc ctccccggct gggtgcagaa tccgtacccc tggatgaagg
    19141 gtgccggcac ctatgtcctg tcgtcgaggt gggagggcct cccgtccgtg ctgatcgagg
    19201 cgctgtattg cggcgtgccg atcgtggccg ccgactgcct cagcggcccc aaggagatcc
    19261 tggagggtgg cagacatgga ttgctcgtga aggtcggcga cgtggatggg ctcgctcgcg
    19321 ggatccggtc ggccctgggg ggcgaggtgc cggcgccggc gcccgagagc tggcgaccct
    19381 acgagcagga gttcgtcgtc agtcggtatc tcgaggtctt ggtgggggag tagccgtgcg
    19441 caagatcgtc ttcgggcttt ctctgatcct gatcttcacg atcccctggg agggagcctt
    19501 ccacctgccc ggcctcggta cgacggcgac ggtgatgggc ctcatcgtcg gagcgtgctg
    19561 gctgttcatg atcgtcgtcg ccggccagat gcgacggccg gtcctgttcc tgttcgccgt
    19621 ggcggggttc gtcacgtggg tcgcgctcac gacgttctgg agccccgatc cggtcgagag
    19681 tctcggcgcc gtgtacctct ggctgcagac cctgctgttc gtctacatcc tgtgggatct
    19741 ctatcggacc aaagcggcgt tgctcgcggg actgcaagcg tacgtcctcg gtgcctacac
    19801 cgccgtcgga ggtgcggtcc tgaactacgt tcagtccaac gcgttctaca ccaatgagga
    19861 ccggtacagc ctcggcaaca ccaacccgga cgggtacggc ttcatcctcg cgctgggcat
    19921 ccccgtcgcc tgttacctgg cggcgtcgcc cgagaccccg aggatcttcc ggttcatcaa
    19981 ctatggctat ctgccggtcg cgttcatcgg ggtggcactc tcgggcacga ggaccgcgtc
    20041 cgtcggggcg gccatcggtc tcctgtacgg cctcgccacg ttgacgaagc tgaggatcca
    20101 tacccgggtc gcggtcgtcc tgctcctcgg cgcttccctg ttcgcagtcc tcccgatcgt
    20161 ccaaccgttg agttccttcc agcgcctcgg ctcgacggga acggagatca cggaaggtga
    20221 cctgaacggc cgcaccggcc aatgggcgca aggccttcga gcgttcatgg agcaccccgt
    20281 cctgggtgtc ggcacggaca tgtaccgaac ggtgaacacg ttgaacaaga ccgcgcacaa
    20341 ctcgtacctg tcggtgctcg tcgaggaagg cttgatcggg ttcacgatct tcctggcgat
    20401 cctcgggatc gtcgccctcc aggtgctgcg tctcccgaag tgggaccgca acttctggct
    20461 gacctcgctg ctcgtgtggg cgatcggtgc gtccaccctc acgtgggagc accgaaagac
    20521 cacgtggctc ttcctcacgt tcgccgtggc cgcggcggtc atcggcgctc ggcgctcggc
    20581 gccgccggac gacgtcatcg atctcaccga ccacgacatg gcggaggtcg gggtcgcttg
    20641 accctgccga ccttcctcgg gatcggcgtg ccccgcgccg ggacgacgtg gctgcatcag
    20701 ctcctctcgg cgcaccccga cgtgtacctg ccctcgcgcc gcaaggaggt gcgcttcttc
    20761 gaccgccacc acgaggaagg acaggggtgg tacgaggggt tcttctgccc tcctgatgag
    20821 gccgggcggt actcggcgat cggtgagatc tccccccagt acctgtactg cgatgcgtgc
    20881 ccggaacgca tctcctccct gcttcccgag gcgaagctcc tcgtgatgct ccggcacccg
    20941 gtggatcggg cgtattcgca gttcggcttc gtgatccagc ggcgggactt ccgcggctcc
    21001 ttcgaggagt tcgtcgcctc gaggcctcgg gcgttggaga tgggcttcta cagccggtac
    21061 ctcgagcgat acctcgatca cttcgacagg cagcggatcc tcccgatcct cttcgaagaa
    21121 gccgtatccg aggcgagcac ggttcgggtc gagctggcca cgttcctggg cgtggccgag
    21181 ggactcttcc ccgatgccac cgagcgggtc aacccgagca cggttccacg cttccgctcg
    21241 atctcgagcg ccgccgtcaa ggtcggtcgg cgcatgcgga ggcacaacct ggagtggctg
    21301 gtggatgtgg cgggccggat gggcctgagg cgcgcattga cgagcgggcg tcgcgtgccg
    21361 gcgctggatc cgcagctgcg gcgggagctg agcggccgct acgagaggga gttcgagata
    21421 ctcgagcgct cgtgggggtt ggatctggcg aggtggaggg cctcgcgggc cactaccccg
    21481 ctcggggatg gcagcgccgg ggtccgacag ggggggatgt cgtgaatcgc ccgctgaacg
    21541 tcctgctcgt ctcgcagccc gtgagctacg gggtcgcgat ctgcgtccgc gacctgacac
    21601 gggcagccgt cgcagccggt caccgggtca cggtggtgag ccccgactcc acgcacggtc
    21661 cgctcgccgg ctggatagtg gcggccgggg ctcgacacct tccgctgaac atggctcgga
    21721 caccgagcct ccgggacgtc tccgacctcc ttgccgtccg gcgcctgagc cgaggccgag
    21781 acgtggtcca tctccattcc tcgaaggcag gtgcgctcgg gcgcgtcgcg gcgatgtccc
    21841 tggggcgacg ccatcgaccc gcggtcgtct tcaccgccca ctactggtcc tggctcgtgg
    21901 ggggacgctg ggccggtctc tatcgatgga tcgaacgggt gctcgctcgc cggtgcgatg
    21961 cgatcgtcgc ggtctcggac ggggaggcgg aggaagggcg tgccgtgctg ggcgctcgcg
    22021 cgccgttgcg atcgatcccc aacggggtcg atcgcgatcg gttctctccg atcggcgacc
    22081 gagccgaccg gaccgaagac gtgccgctca tcgtctgcgt gggaaggctg agcgaacaga
    22141 aggggcagga tgtggcgatc cgcgcgctcg ccctcctggc cgatcggacg gttcgtctgc
    22201 gcctcgtcgg cgacgagagc gggcacggag aacgcgcgcg gctcgacgcc ctcgcggcct
    22261 cgctgggggt gtccgagcgg atcgaatggc ggggtggcgt cgatgcgacg gcgccggagt
    22321 accgtgccgc cgacgtcgtc gtcgcccctt cacggtggga cggcctctcc ctcgccctcc
    22381 tcgaagggat ggcgtgcggg gcggcgatcg tcgcgaccga ggtgaacggg agcgagtgcc
    22441 tcggagaggc cggccggatc gtgtcgccgg acgacccgga gtctctcgct caggccgtgg
    22501 ccgatctcct cggaagcgcg cccgagaggg cccggttggg gcgggccgcc cgagaacgca
    22561 gccgctcctt cgatctcgcc acgacgatgg gacggaacct cgacctgtgg tcgggcctcg
    22621 cgattcgtgc cgccggtgac ccggatgccg ctgccacgca ggcggtcgcc gaggatgggg
    22681 ccgaggatgg ggccgaggat ggggaaggag ccgctcgatg gaccaacacc tcgacgtctt
    22741 gatgaccgaa cgaccggccc tggacgctcc gatcccctat gtgtgcatgc cgggcagccc
    22801 gtacacgggt tcgacgctcc tcgggatgct cctggggaat catccggaat gcgcgtcgat
    22861 cggcgcggcc accgggctca ccgcccgggt cgatgtctcg acgtacctct gctcgtgtgg
    22921 cgcgagattc accgattgcg ggttctgggg ccgcatcgcc cggagaaccg tggagctcgg
    22981 gcacccggtg acggtgttcg agaaggactt ctggaacacc catgtacggg tctcccggcg
    23041 gcgatggctg aacggactcc tcgtgagctc cctcggcagc gatgtgctga cctggtgccg
    23101 ggacgccgtg ctgcatcggg ccgggccggt ggcgcggaag atcgcggagg ctcgatccgc
    23161 ttcgtggagc ctggcacgcg ccgtcctgga gcagacgggg aagtccacgt tcgtggacac
    23221 ggcccgcgat caccagcgtc cgcgctatct ggccgacatg ccgcgtctcg acgtgaaggt
    23281 catccacctg gtgcgggacc cgaggggcaa cgtggcgagc atcatgaagc acaccaaggt
    23341 cagcgtcgag aaggcagccc gccaatggcg gcactacaac gtcgaagcgg atcgggtgag
    23401 gcgacgcttc cctcccgagt cgtggatgct cctctactac gaggaactgt gcgcggaacc
    23461 acaggcgtcg ctcgaccgcg tggcccggtt catcggcgtg cagccggcgc cgattacgca
    23521 gctgctggag aactcggaac gacatgtgat cggcaactcg atgcgcctca aggtactgga
    23581 cgagatcagg gaagatctgt cgtggcaacc gaccctgacc tcggtcgatc tccggacgat
    23641 cgctcggatc gcgggaccga ccagccggcg gctcgggtac gagtggccat gagtccgacg
    23701 agaccgctgc tcgtgttcgt gcacatcaac aagaccgcgg gtacgacgct tcggtacatc
    23761 ctccgcagct cctacggttc acgacactgc gatgtcgagc cgtggcacgg cgcatgggtc
    23821 gaccccccgt tctcatcgga ggatctccgc cgcgtgcgga agctctatcc gcgcctcgcc
    23881 agcatcgcgg gacaccggat cagcggccac gtcgatctcg acgaagaagg aaccgacttc
    23941 cggtacctga cgttcctccg cgagccgatc gcgctgtgtg cctcccgatt ccagtaccag
    24001 ctcgactatc ggaagaagca ggatctcgtg ttcgaggact ggatcgagaa ggcctgggcg
    24061 cgagacgcgc agactcagcg gatcggcggg accaccgacc catcggatgc gatccgcgtg
    24121 atcgaacgga agtcgatgtt cgtggggctc acggagcgat tcgatgaatc gatcgtgctc
    24181 ctgcaagccc aacgcgcccc cgatctgaac atcgcgtacg cgccggtcaa cgtggccaag
    24241 agctccgata tcgcgaaagg actgctggcc gacccggcga cccgtcagat gatcgtcgac
    24301 gcgaacgcgg ccgacctcga gctctacgac cacgtggtgc acgagatcta tccagcccaa
    24361 cagcgagcct atggggcttc gctgtcgagc acggtcgcga ccttccgaga tggagtgcag
    24421 cagcgatacg accgccggaa gctcgcgctc tacgggctga agcagcgcgc gatgctgaag
    24481 ccaacgctcc gcctctaccg aggaaggagg acgggcggtg tcgttcgggc actgatcggg
    24541 tgaacgatca cgccggcagg agccagtggt ccaggttcct ccctatcagc gccgccgtct
    24601 cgccgatgtc gtcccggaag tgccgcagca actccgctcg cagttgatcg gggagcgggg
    24661 gcgccttctt caggttcttc gtccgcaacc gtctgacggc atccgacacc ctccgcggga
    24721 tccagggctc gacgaccctg cggacggacc tgctcgtgag gacccgctcg agggttcggc
    24781 tcgacggcag ccccccgatg ttgtgcggcg tctcgaggtc cggggcgaac ccgggatcga
    24841 cgccgagcac tgcgaagacg gcgcgcacgc acgcggcagc gtcgttcttg agatcgtcga
    24901 agaggaacac gtcgatctgc tcccgcggga accgttcgta gtagcgggag agcgcttcgt
    24961 ggtacctgct gatctgcatc cagtgggact cgggacgcgc ccagggggca tcgggcgtca
    25021 ggtcccggtc ggggtccaga ctccgacccc gcgcgcggag gtacatcagg tagtccgagt
    25081 aggcgcgatc gacgggctcg cggaggaagc agacgatccg ggcgccgggc accacttccc
    25141 ggatccgggc ggccgactgc gggcactcca ggtagatcgg tgatgcttcg cccacggcga
    25201 gtgccccctc cgatcctgcg aagagcgctt cgtactcggc gaaggatcgg atcgggaacc
    25261 ggtggagctc gggatctccg tacagggggt ggccgtcgtc gtcgagtccg aacgcgaagt
    25321 agttcgtctc cttcaccggg ctcatgaaga cctcggggtg atcggccagg taccagtaca
    25381 gcgcggtcgt gcctgctttc gcggcaccga tgatcacgaa ggtgggcagc atggcggtcg
    25441 ctcagacctc cctgccggcg tcgatcagca cctcgatctg ccggaccacc tgatcgaggt
    25501 cgcgatccgt gtcgatgggg tacacggagg ctccgtcgaa ccggagcctc gagctgagct
    25561 cgtgacggaa ccgcacgtag tcctccggtt ccttcttctc cctcgccgcg ttccgggcca
    25621 gcgttacctc gagaggggcc gtcaggtgga agaccagatc cggcgtgggg atatcgcgat
    25681 acagacgccc ctcgaccgtt gcgaggatcc gtcggaacga gaaggcgctc ttcggcatcg
    25741 gcagatgggt gagctgcggg ccatcgggcg caccgctcgc gtccgacggg taccggtcgc
    25801 tgagcacgac cgtccccgct ccgtcgcgca ccgctcgcct gaggagggct cgccgctcgt
    25861 acgcgagcat cacggatcgg atcccgaaga ggagcgggta cgtcgtgccg gtcgcatcgc
    25921 ccccctcgta gcgttcctcg acacgcagcg tccgttgttc cgggaacagc gagcggagcg
    25981 ccggcaggag cgcgtgcggg atcgaggtca ggggcgtgct cggcggcttg ccggcatgga
    26041 cgcggcgaac gcgccgcctc ttggacagcc accgctccac ctcgtccagg acggtcgact
    26101 tccccgtcgc ctccgatccg acgaacgcga tcacggtcgc tctgcgggat gccgaccggc
    26161 cgccggctgc cgagcgggtc gatgagccca gcgcgcgcgc ccgcgtctcc accgcgtcgc
    26221 gcagccggcc gacggctcga tcgagaccct cgggcgatgc atccgcgagc tcgatggtca
    26281 ccacacgatc gcgtatcgct tcggactcgg ccagcgcgtc gaagacggtg atcgccctcc
    26341 ggatccatga tccgatccgc tcctcatctt gccccgcgag ctcccgccga acgtccggac
    26401 ggatcatcgc tcgctccacg agcaggtcgg tcggcgcctt cacgtggacc acgaggtcgg
    26461 gaagcgggac gaggcgggag aagcgctcga tctcggacgg cgtgggtgcc gcgtcgctgt
    26521 agacgaacag ttggtacgcg atcaggatcg tgccctcgtc gttcaggaac gtgacgctgg
    26581 gatcggccca ccgggtcagc tcgtgcacgc cgaccttccg gacgacgttc cggagatagt
    26641 tcgccttggc gaaggtcgac ggggcgtggt ggcggagccg tcggaacgcg aaccggacga
    26701 aggcccggtg atcatggaac gacctgagga acgggggcag cgcggcagcg tcggccagca
    26761 ggttgaccgc gtgggggttc tccacccatc ggcggccggg cctgtcgatc accaggtccg
    26821 tcgccatccg gaccgagccg ctctcgaggc cccggcgcag cagctcgcgc gccagcgtcg
    26881 atttcccggc gccggtggcg ccgatgaact cgacgatcat gccgacgccc cgctcccgac
    26941 cagaggcgcg acctctcggt agacggcttc ggtaccggcc cgcatgatcg gcaacgtgaa
    27001 gcgggccgcc atgtgctccc tgcagcggag cccgacctcc cggcgctcga tcggcgtcat
    27061 cgcacggaag cggcggatcg cgtcggcgaa tccgtcgacg tctccggggt cagcgaacgg
    27121 gatgaagtcc gcaccgtccg cgagctcgcg gtgcggcggg atgtccgaga ggatcaccgg
    27181 gcagcccgcg cccatcgcct ccaggacggc caccgggagc ccttcgccgt gcgaggtcga
    27241 gacgaagacg tcggcatcgg cgcaggcgac gaagacgtcg tctcgcggaa cgaggcccgt
    27301 gagcacgata cggtcctgca gacgcagctc gcgagccctc gcttcgatcg tcgtcgagag
    27361 cgttcccgca ccgacgagca tcgctcgact gcccgcgtca tcgccgactg cggccgcgaa
    27421 cgcgttcacc aacgtgagcg ggtccttcac ccgctcgagg cggcccaccg agatgaccgt
    27481 gaacccgcct cggctcggcg cgggacggcg ggtggcgatc gcgcggtcca cgcgatcgag
    27541 gtcggctccg ttggggacga cccgcgatcg ccggcgtacg aggcggtcga gggggcgggg
    27601 aaggctctcg tacgccgatc gactgcagaa cacgatccgc cggaaagccg cgacggagat
    27661 gatcatcatc acctggttcc ggagcttgta gtcgtggaag gagtcctgca cggtgtaaac
    27721 gagcgaggaa cgcacgcggg agaagcgccc ccacgcgagc gcggcgagga cgaggagggt
    27781 ccctgtctgc ggcgcatgcg cgtggatgat gtcgtacttc gcgcggagcg ccgccttcag
    27841 agcgcggaag aaccctcgca gggtgccgtc gcccgcgaag agggtgatct cctgaggagg
    27901 cgtcagcttc ggggtgaagt aggtgacgat cgagatgtcc cgttcgtcca tgagcgggag
    27961 gcaatgctcg ttgtactgtc cgttcgtctc accgagcatg atgatcaggt gcagcacacg
    28021 gagcttccgg gggggtgcgc tatccgccag ggtggttccc ctccctccgc cgcttcgacg
    28081 ctcggacgac ctccacgatc tgcgccactg tggacgtgct ggtgtcgaac cgcgtcgtgc
    28141 ggacgtcgtc ccatgcctcc acgaccggga tgatgctctc gaacgctcga cggtagcgag
    28201 cgatgaagcg gtgtccggcc tctgtccgcc cacctttctg ccggtgatcc tggtcgcgca
    28261 cgttgatacg actccacagg acggcgtcgg gcgcgtccag ccagatgacc tcactcagct
    28321 cgttcgacca tcgatcgagc atctcgcgct tccaggacgt gaaggagcgg cgttcggtga
    28381 acggcttcgc ttccgcagat aggcggacga tcgcgtacag aggcccctga tcgagcagga
    28441 ggacggtgtc ggtctgcggc cgtctgcgac ggagcacggg agcccatcgg gtgacgtaca
    28501 cgagcagctt gtactccggc caactgatcc gcggggcgtg cgtgagaccc gcccacagga
    28561 tcggcagcag ccgtgggatc gtgcggagga tcaggagcag gtcggctggt cgacgggcgt
    28621 ggatgaaggg ggcgatctcg aatcccgggt cggcggcgag atgcgtggca agcgtcgact
    28681 tgcccgcgcc cgccacgccc aggatctcga tgttgcgatc cgctctgtgg gtcggcccga
    28741 ggcggccgac cgctgtctca acgatccgcg tcaagggccc tccgaccgga tgagttccct
    28801 caagggtaga gggagcacgc tgagacaacc atctgatgat cagcgtagcc gtggtacgcg
    28861 atccgtaggg gtccccggac gaacggggtg ctgcgcacgg ttcctcgggc ggtggaggcg
    28921 cggggaagga gcacatcacg taggcgatct gcgtcgaacg catgatgtcg gcgcgcctcc
    28981 cggccgacca gactgttggg gtcgcggggc gcgcgataga cgcacgacag ccagggtggg
    29041 acctctttgt gacgagcatc ctcaaacacg ctgcgcgaag gcgcctgatg ttcgtcgtct
    29101 cggcgctctc cgtcatcgtc atcgggaccg cgcttccggc atccgcgaac ctccccgata
    29161 cctccacgcc ttccctcgag ctcgatcgga cgatcacgac ccagccgttc gtgggcagct
    29221 cgaccacgat gcgtgatggg gaaggcagcg cctacgtcac gccggacgga tcgctctggc
    29281 tcacggagga ccacgagaac ctcatcttcg agacgaaccc gcataccggt gcgctgaagc
    29341 gcgtgatcga caacgccgcg ttccagaacg caccgctctt cggcggcgga ccggtggctg
    29401 gtcctaaccg gacggacgac ttcgagtcga tggcctacga cgaagccaac gactggctct
    29461 tcgtgttctc gggcccgtgc tgtgacgcgt cgcacctgcc caccgtcttc cgactcgtcc
    29521 gcgtcggcgg cgttctccag gtcgactcgt accaaccgtt ggcggcgacg gcgaacaaca
    29581 ccgcttcggc gtggaacccg gccgaccaga cgctctacgt tggccagggg cagcagatcc
    29641 gaacctatga ctacgtgacg aacacgaccg ggacggcgtt cacggtgacc ggactcagcg
    29701 ggatcctggg cttctccttc tcctcgcccg ccgacacggg tggcagcgat ctgttcgtgg
    29761 tcacgaacgc ggaacggctc cgccgcgtgg attgggcgac gaagacgctc gttcccggct
    29821 ggacgttcga gctgacgccc ttcgaggtgt tcgacagccg cgcggtggag ctgatcgacg
    29881 aacagttcta cgtgctggac ggctacgact cccgcccgcc cggcccgctg cggttcgccg
    29941 tgttcgtctt cgatgtgatc gggccccaac ccgtggctcc cgtggcgtcc ttcaccgcca
    30001 cgccgacgaa cgggagcgct cccctcagcg tggacttcga cgacacgtcg acgaacaacc
    30061 cgacgagctg ggcgtgggac ttcgagaccg acggcaccgt cgattcgacc gcgcagaacc
    30121 cgacccacgt gtatcagacg cctgccacct acacggcgac cctgaccgca acgaacggtg
    30181 cgggctcgga caccgagacg gcacagatca ccgtcaccca ggcggtcgcg ccggtggcct
    30241 cgttcacggc cacgccgtcg acgggtgacg cccctctctc ggtgggtttc acagacacgt
    30301 caacggggaa tccgacagta tgggcatggg acttcgagtc gaacggcagc gtcgattcga
    30361 cgtcgcagaa cccgacccac gtgtacacga ccccggccac gtacacggcg accctgaccg
    30421 tcacgaacgg tgccggttcg gacaccgaga cggcgcagat caccgtcaac ccgccggcgc
    30481 cgccgacgaa ccttgtgggc aacccggggt tcgatgtcga cacggcggga tggagcacgg
    30541 cggggtcggg gacgctcgtc acgctcaccc gggtgacccc cggccgcgac ggcacgggag
    30601 gggcggccca cctggtcaac ggagcgaccg gcaagcggaa gtgcgtgctg aacgatcagc
    30661 cgaactgggt cacgacgacc acggcgggga cgtacacgtc gagcatctgg gtccgcgggg
    30721 acgccgcggg cgccctgatc aagatcaaca tcagggagat gaacggctcg accgtcgtga
    30781 agtcccgggt ggggaacttc accctgacga ccgcgtggca gcagatcacg gtgtcgcact
    30841 cgatcctctc gccgggctcg accctcgatc tccaggtatt ccttccgaag gcgcaggccc
    30901 ccccgggaac atgcttctac gccgacgacg cttcgatcgt caggtcctga tccgcgcccc
    30961 gcggcgggtc tcggcggact ctgttcgact cggtacgttt cctacgtcga agcgcgcatg
    31021 catcgagccc gatggatgcg acaatctctc tctaccgaac ggatcagagg gagcgccgtc
    31081 gatggacacc cccgagtcct cgatcggctc ggatgggggg gtctgaacgg atggccctcc
    31141 acgagcctct cgagatcgaa ttcgctcaga cgccctcgct ccgctcccgg cctccgttgt
    31201 cgagcctgct ccccgcgatg gatgccctga cgctggtcgg ggtatcggcg atcctggggt
    31261 tccccctcgt ctcgatcgcc tacggggtgg tcacgttcct gatcttgaac ggcgattcgt
    31321 cccgcaagta ccggatcaac ccacggctcg gtgacgaggc gggttggttg ctcggtcggg
    31381 tgtcggtcgc tctcatcgtc ctcctctcgg tcggatgggt cgcccagctc gcgcacatcg
    31441 acctcctggg gagcgggatc cgcgagctca cgaaggtcgg gctggccgtg gccgcggctg
    31501 tcttcctggg ccgaggggcg gcctacgcgc tgggtcggaa agcgaaggag cgcggcctgg
    31561 cctcggagcc caccctgatc gtgggcgacg gggaactggc gatgggactg gccgagacgt
    31621 tcctggagca tccggaatac ggtctgcggc cgatcggctt cctcgggcgg cccctcgtcc
    31681 gccatgtggc gttgccgctc ctcggggagg tccggaacgt cgagcgcgtg atcggcgact
    31741 tcgaggtgcg gcgcgtgctg gtggcgttcg gcgagatgcg tgatcaggag ctcgtgacga
    31801 tccttcgggc gagcgagcgg gtgccggcgg agatccatgt ggtcccgcgc ttcttcgagc
    31861 tgagcggcgt tccgcagggc gctcccgttg acgacgtcgc cggcattccc ctgttccacc
    31921 tgcggcgccc cgccctccga ccgggcgctc gtttccagaa gcgagcgttc gacatggcgg
    31981 tcgcatccac gctgttcctc ctgagcctgc ccgtgttcgc ggtcatcgcg ctcgcggttc
    32041 gcctgtcgag cgaaggaccg gtcctgttcc gtcagaagcg ggtgggcctg aacgggaacc
    32101 tcttcgagat catcaagttc cgaacgatgt acgtgaacga cgagtcggac accgcgtgga
    32161 tcgcggagag ggatcacgag cgtgtcaccc ccatcggaca tatccttcgg gcggcgagcc
    32221 tggacgagct cccgcagctc atcaacgtga tgagaggcga gatgtccttg gtgggtcccc
    32281 ggccggagcg accccactac acgggccgat tctccgagac cgtctcccgc tacgacgatc
    32341 ggcatcgcgt cgtggggggc atcaccgggt gggcgcagat caacggtcgg agcagagggc
    32401 tcgactcggt tccggagcga gcacgcctcg acaactacta catcgagaac tggtcactgt
    32461 ggcgggatct cgtgatcgtg gcccggacgg tcgcggtcct gttccggggc cgctagctct
    32521 cgttcagtcc gtgcggcccg gcggtgggtg gccgcgggcg atcagggcct cgtcgatccg
    32581 ggcggagccg aattcctcga tgagccgccg caggtcgcgg cgcatcctgc cacggccggt
    32641 gtaggcgcgc agacggaggg atcgcgccat ctgcgggaga cgctgctcct ccgcatccag
    32701 ctcccagggg tggaggtaga aggtgaacca ggacgtggag cgcaggcgct gcgcgacgcc
    32761 gcgacggaac accggatagg ggatcagccg gaaataggca cccccggccc acggcacccg
    32821 cgcgggcccc accggaagtc gggtcatctg gatctcgagc agtcccggcc tgacctcgac
    32881 cacggccgca tcgggatcga ccgtggcgga gatggttcca tagcgatcgt gctgcgcgaa
    32941 tccgaagaag ctggagtcgt accagtagcc tgcctccgcc agcacatcga gcacgtggtc
    33001 gctcaccgag aagttcggcg cccgcacccc tcggaccggc gttcccgtga cctgctcgag
    33061 cgcgtctctc gcgtccgcga gctcccggat catctcgtcc gtcgtcagct ggttcaggtt
    33121 gcggtggagg tgggtgtggc tcgcgacctc gtgcccgtcc tcgatgatcc tgcgcacgag
    33181 cgcggggtac cgctccgcga cccatcccag cacgaacatc gttgaccgcg ctccgacgtc
    33241 ggcgaggatg tcgaggagcg cgtgcgcgtt cggttcgaga cgtggctcca tcgacgccgg
    33301 gtcgatggag ggcaggctga cacggacgtt ctcggcgtgg aaccagtcct cgatgtcgaa
    33361 agttgcgagg aatcgaggcg ccgtgtcggg cgcggtagcg ggttcgtcga tcgttgggtc
    33421 gccctcctcg ggaagatgct ctgcggcacc ggtgatcacc ggggataacg cccgccaatc
    33481 tagcggggat cgcctacggt ggtcaccctc tcggcgacgt atgtgacgta cggcagccga
    33541 gaagctcccc gaggagacct ccactcgggg gacttgcgca tccgggcgaa gctagtaggg
    33601 tcctgcggat gttccggcgc gcacgcgacg gcagacggcg gcgcggatcg ctgctcctgg
    33661 tcgtcatcac gctcaccacc ggcctggtgc cgctctcggc gccgtctgcg tcggcgagtg
    33721 cccggatcca catcgagtgc gacttccacc atcgcaag
//
LOCUS       DASUYD010000033        26684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_679791, whole genome shotgun sequence.
ACCESSION   DASUYD010000033 DASUYD010000000
VERSION     DASUYD010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 26684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679791"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF195_01660"
     CDS             complement(<1..378)
                     /locus_tag="VF195_01660"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6579568.1"
                     /translation="MNDGPSPRGGPPRARALTIAGSDSGGGAGIQADLKTFSALGVFG
                     MTAITAVTIQNTKGVAGFEELSPTTVAEQIRAVAGDIGVDAAKTGMLASAAIVEAVAE
                     SAAEMSLPNIVVDPVFVSKHGHPL"
     gene            complement(375..1358)
                     /gene="thiL"
                     /locus_tag="VF195_01665"
     CDS             complement(375..1358)
                     /gene="thiL"
                     /locus_tag="VF195_01665"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEX6579569.1"
                     /translation="MDLTEDELIAAIGRVLSGAGPEVLVGPGDDAAVVRGGSGEVVLT
                     TDAMVEGVHFLIDRTTPRDLGYKAIAVNVSDIAAMAGSPRFALCALTLTDAIDASWVV
                     ELAAGMRECCDEFAVSLVGGNLSRGEELAIVVTVTGEVAPGRAVRRDGARSGDRVVVT
                     GSLGGSAAGLRVSSQRSWSDDERDALRRSMRPVPRVGEASILSAHGATAMMDVSDGLT
                     IDLSRLCRASGVGARVEVAHVPVHPASTVHEALGGGEDYELIATLPGPDAVAGAARDV
                     HEAFGVSLRDIGVIIEGGGARSAARSAGGLVAVDEDGTERPLEIEGWDHFG"
     gene            1522..3021
                     /locus_tag="VF195_01670"
     CDS             1522..3021
                     /locus_tag="VF195_01670"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEX6579570.1"
                     /translation="MSDVFADPTPEELRRFTEAMPECRITEFGNMNVQTRVVSRSADS
                     TFVVDRPSSGKTMTRDAYDKIARRQDAYLADHDVIRIDGWIGNDAELRTRARLLIEKR
                     YANIAGMQQKLYFPRDDDADPDVQVIYTPGLTAPGYPGDRVIAVDLDANVTRVLNSDY
                     FGESKKGGLRMWNNIVYEKGGLALHAGLKVIPTSAGDKVFMIIGLSGTGKTTTTFTTQ
                     NGSKPIQDDFVGLMPGGRAFGTENGCFAKTFSLDPEFEPSIYGAVTKPTTYLENAYQD
                     DSGTVNFFEESYTQNGRAVFEMRDLQTFEDARNVGPVDYLLILNRNENIIPSVAKLDQ
                     EQAAAYFMLGETTGTSAGGAAEEGKFLRVPGTNPFFPLPHGLQGNRLLELLATHPIET
                     YLLNTGRVGGKDGDDRSKKVKIPHTSACVKGIAEQTIVFEEDPDFRYQVASEIPGFDD
                     PELLRPALLYEREGRIEEYRAIVERLKVERVEHLQRFTELSEDIIKAAG"
     gene            3142..4944
                     /locus_tag="VF195_01675"
     CDS             3142..4944
                     /locus_tag="VF195_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6579571.1"
                     /translation="MGHEGHGGHEGHGGHEGHGGHEGHAATFRDRFWLSLALSVPVLL
                     FSEMIQDWLGFSLPTFPGDDLVAPILGSAVFLYGGQPFLTGALDEARARRPGMMLLIA
                     LALIVAFGASVASAVGWLDLEFWWELALLIDVMLLGHWLEMRALGQATSALEALASLL
                     PDTAELVTDSGTEEVTLDALRPGDVLLVRSGARVPADGTIVEGEAAFDESMITGESRP
                     VTRGPGDRIVGGTIATDSSVRVRVTAVGEETTLAGIRRLVAEAQASRSRAQALADRAA
                     ALLFYVALASGTITLIAWLAAGEPDQAMERTVTVLVIACPHALGLAIPLVVAISTAMS
                     ARDGILVKDRLALERMRTVDTVLFDKTGTLTTGTPAVREVLADSGDEDTALSLAAAAE
                     ADSEHPLARAIVEEARRRRLDVPSASGFRSITGRGIRADVLGSEIAVGGPALLRELGV
                     EMPERSSDTVADWRRRGSSILTVVRDGKPVAMIALEDTVRPESRQAIADLRNAGLEVL
                     MITGDAREVAEAIGRRDLGIDDVRAEVMPEHKEAVGGGRPRSLGRGARPGAGAVAMSL
                     STVVVAFNALLLRRLDLRPGEAQAPLETAPADTR"
     gene            complement(4874..5224)
                     /locus_tag="VF195_01680"
     CDS             complement(4874..5224)
                     /locus_tag="VF195_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579572.1"
                     /translation="MSDVELVAVALFATLGVRSVVHWLRHPIDDLVGRRDLVLYALFV
                     MARAGVWFGLMGLFLLFASVETQGRAFTDDAAELNWYIVVLLVPISLQFVTGYLLGRS
                     RGGPGPPPDGDRAA"
     gene            complement(5221..5862)
                     /locus_tag="VF195_01685"
     CDS             complement(5221..5862)
                     /locus_tag="VF195_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019521331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6579573.1"
                     /translation="MIERVFRDREEAGEALAAAVARVVAEPATVLAIPRGGVTVAGPV
                     ARNLGAPLDVVIPRKLGAPGNPELAIGAVAPGVRVLHERAVRQLGVSMEYIQREAALQ
                     ETEIARREAAYREGRPPAPVAGRTVVVVDDGVATGATATAALRWARAAGASRVVFAAP
                     VGPPAARASLEAEADDVVLLREPFGFLAVGEWYRDFDQVTDGEVLRVLREQHG"
     gene            5937..6170
                     /locus_tag="VF195_01690"
     CDS             5937..6170
                     /locus_tag="VF195_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6579574.1"
                     /translation="MVHAYILIQTEVGKAASVANAVRNTTGVTEAEDVTGPYDVIVRA
                     EADSVDDLGKLVVARIQAVEGITRTLTCPVVHL"
     gene            complement(6208..7320)
                     /locus_tag="VF195_01695"
     CDS             complement(6208..7320)
                     /locus_tag="VF195_01695"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HEX6579575.1"
                     /translation="MKRRRVAVLFGGRSAEHEVSCLSARSVIDALDPERTEVIPIGIT
                     REGRWHRVAGPPALPSETGRMPEVTDGSGGSVELAAEGMSAEIVGADGSREPIDVVFP
                     VLHGPYGEDGTVQGLLELAGVPYVGAGVLASALGMDKDVQKRLFVAAGLPVGPYEAVR
                     ESGWVDDPETIEAAAEALGYPLFTKPATLGSSVGVSKVHDNEELAAGLEEAFRFARDA
                     LVEKAAEGAREIECAVLGNDDPVASVAGEIVPQDHEFYDYEAKYLDEHGARLLIPADL
                     EPRVLEDVQRLAVAAFGALKCAGMARVDFFLHGTDELWLNELNTIPGFTSISMYPKLW
                     EASGLSYGDLVERLLDLAVERHEAERKSAGVRALGL"
     gene            complement(7317..8504)
                     /locus_tag="VF195_01700"
     CDS             complement(7317..8504)
                     /locus_tag="VF195_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6579576.1"
                     /translation="MSDPGKRRRFQTRAVHPHRPALPEQSSSSVPIFQSSTFRFETNE
                     EFARAIRFDGPGYVYSRGYGNPTVDAFQSAMADLEDAESAIGFASGMAAISSLLLTVA
                     SAGSRIVAGTALYGGTVSMLQHVLPRFGIETTFVDPTDVDQIRDALPGADLFYCETIV
                     NPTTTVPDLAVLAELCRDSGVLSAVDNTFASPYLCTPISLGFDVVLHSATKYIGGHAD
                     LIGGVACTNEDLFRRLRATVIDLGGSMQPLEAWLCLRGLSTLGLRLDHHCASAGRLAE
                     ALDAHPSVERVHYPGLVSHPQHEIAARQLRGFGGTLTFEVAGGVEAGSRVAEALELGW
                     IGGSLGSVMTLVAHPASTTHRQVDPDARRSAGIADGLLRVAVGLEDADDLIEDFTRAL
                     DKA"
     gene            complement(8515..9147)
                     /locus_tag="VF195_01705"
     CDS             complement(8515..9147)
                     /locus_tag="VF195_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6579577.1"
                     /translation="MALPPLRFWFRLSYEHLDRIPRNGPAIVACNHISYLDPLTNGDA
                     IVRARRRPRFLAKGDLFKIPLVGRALRGAGQIPVTRGTRDRSSLDLAVAALDRGEVVV
                     VYPEGTVTKRDDGLPMEGKTGLVRVALASGVPIIPMASWGSRAVWQKSGRGSLRFGRP
                     IWTTVGEPIPLPAEPDGGQVELRALTGEVMDAITRLAVDLRDRYPRRWTK"
     gene            9241..9885
                     /gene="cofC"
                     /locus_tag="VF195_01710"
     CDS             9241..9885
                     /gene="cofC"
                     /locus_tag="VF195_01710"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HEX6579578.1"
                     /translation="MRAIALPVKSLGRAKSRLSSLLAPLERGALALAMFEDVLDATLA
                     VDGWETWVVSSDESVLEIALARGAHGISELRPTLRSATLQIEAEAAARSTEALAILLA
                     DTPLVTLEALRAALHTLGPVVIAPSSDERGTNLLLRRPPGAIPNRFGPDSFRRHLQTA
                     AERGLPTAVVEREELSFDVDEPGDILTVLETRRPGRTLDVCRDMDLAGRLAART"
     gene            9897..10106
                     /locus_tag="VF195_01715"
     CDS             9897..10106
                     /locus_tag="VF195_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579579.1"
                     /translation="MDQGTIKDFDEEGRTGVLLTDERAEVVIDTDSLDASGIRTLRIG
                     QRVKFETEERDGATVARSLRIVTWD"
     gene            complement(10117..10407)
                     /locus_tag="VF195_01720"
     CDS             complement(10117..10407)
                     /locus_tag="VF195_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX6579580.1"
                     /translation="MNKGGLVTEVSKKTGLSKAEVGRVIDAAINTVRETVVEGDRVTL
                     ADFGTFERKHRNRRIARNPRQPDVPIVVPERDLPAFTPGKEFRHQVLGNNSG"
     gene            10569..10639
                     /locus_tag="VF195_01725"
     tRNA            10569..10639
                     /locus_tag="VF195_01725"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10601..10603,aa:Gln,seq:ctg)
     gene            complement(10873..11901)
                     /locus_tag="VF195_01730"
     CDS             complement(10873..11901)
                     /locus_tag="VF195_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEX6579581.1"
                     /translation="MSALKRVRWSSPGITRRRQGRGFTYLHPNGQPVRDEATRERIRA
                     LAIPPAWEDVWICPDDRGHLQAVGTDAAGRRQYLYHEDWRARRDRQKFDRVLRLARRL
                     PAMRRICERDIVSPGLTRQRALAGAVRLLDLGFFRIGSESYTEVNGSYGLATIRRDHV
                     RVRGDEVRFDFSAKGGQRRIQTIEDQALARLTRALLRRKGGGKEFLAFRDGPEWRDVR
                     SEDINGYLKDLAREDISAKDFRTWHATVLTAALLASEEEGLRSVTSRKRVVSSVVKEV
                     AEALGNTPAVCRASYIDPRIIDRFLNEETIALPRSRDLTDDRVRAAVESRVLDLLEPA
                     SARPRRVA"
     gene            complement(11898..12902)
                     /locus_tag="VF195_01735"
     CDS             complement(11898..12902)
                     /locus_tag="VF195_01735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6579582.1"
                     /translation="MTEPKEGAAQRLLHRADEVQRAHRALAFPFAVVKKFGDDRASQL
                     AALIAYYGFFSLFPLLLVFATVAAFVLDGNPDLQKRLIDSILEQFPVVGTSIGGVIER
                     SVEQLSSSPLALAVGLVGALWTGTAVVAAAQQAMDDIWDVPRAERPGLLGRIVRAFLL
                     LFVFAASIVLSTFLAGTGTETGWGSVVLRAISLVGLTLVSIAVFAFAYRVLTVASVAW
                     RQVLPGAVVAAVGWTALLLVGRWIVDRQIGHASAVYGLFATVIGLLAWIYLAAQLFLV
                     AGEVNVVLARHLWPRSLAPPPLGTKDHEVLAGQAEEQRARPEERVDVTFVDPSGDDRR
                     "
     gene            12978..13229
                     /locus_tag="VF195_01740"
     CDS             12978..13229
                     /locus_tag="VF195_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579583.1"
                     /translation="MSESGTVGSGGLCPVCGVGTLADIDFGGDENVQDTESRQVDTYT
                     CGHEIERAPLETADAERLDVEQRTSEETTSPTPEEGTAP"
     gene            complement(13226..15274)
                     /locus_tag="VF195_01745"
     CDS             complement(13226..15274)
                     /locus_tag="VF195_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX6579584.1"
                     /translation="MAWIAILSIFLPLVSATGALVALYATTSLPEIPPLAETTVLLDR
                     NGNEVAELHAGVDRVLIPFSQMPESLREAVVAVEDADFYRHDGIDKLALVRATWRNLT
                     SGSLTQGGSTITQQYVKNVLTGPERSVSRKVREAILAVKLEDTTSKHEILRGYLNTVY
                     FGHGAYGVQAAARTYFDRDAKDLTLLQSATLAGLISAPSARDPFEHPEEARLARDLAL
                     TRMAEVGSIGLARAELLRARPLGLAEEANVRSGAPHLMEHVRLDLKAGYGLDALYRGG
                     LRVRTTLDMEWQRAAERAIGISLPSRSDPEAALVAIDPRNGAIRAMVGGRSFARSEFN
                     LATQARRQAGSAFKPFVLVAALERGISPLEVRAGPSSITIPDPFCETGGRPWSLSNAG
                     DQSAGTMSIENATAGSVNTIFAQLTVEVGPEAVAEVAHRMGIRSRLAPVCSIGLGTSE
                     VTPLEMTSAYATLAARGSYVEPTAIDRVTGSDGEVLQGPLRGLASVGSEVLSPQDADA
                     TTRVLQEVVDHGTGTAARLIDRPAAGKTGTAQDATDAWFCGYVPQLVTCVWVGYPDGA
                     RPMRDVAGVPEVYGGTIPAKIWHDFMTEATEGMAVMGFPVASYAVYADPPPPEPSPVA
                     VPAVPSATPSPSPSPSEEPSPSPSSEPSPSAQPSPSPSAEPSPSENPAMPLGTRSRDP
                     "
     gene            15523..16977
                     /locus_tag="VF195_01750"
     CDS             15523..16977
                     /locus_tag="VF195_01750"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008493307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX6579585.1"
                     /translation="MDTSTQRIPVTLVPGDGIGREVTQAARRIVEATDAPIDWEETEA
                     GAEVFKKGLPSGVPEETIASVSRTRVMLKGPLETPVGFGEKSANVTLRKLFETYANVR
                     PVRELPGVKTPYSGRGIDLVMVRENVEDLYAGIEYMQTPGVAEALKLISVKGCEKIAR
                     FAFEYARSEHRTLVTCATKANIMKLSEGTLKRVFEQVAPDYPDIESWHLIVDNCAHQL
                     VKRPEQFDVLVMTNMNGDILSDLTSALVGGLGFAPSANIGSEVAIFEAVHGSAPKYAG
                     KDVINPTALILSTVLMLRYLGLFAQADAIEHAVLVTLEDGVLTGDVVGYGNGASTTRY
                     TEAIIGNLGRRSEEWTVREHRPLELPKLSDAIDYVRPESRAVVGMDVFIESPMGPNEL
                     GASLMELTHETPLSLKLVSSRGTKVFPPTGAITDTVDHYRCRFIIKESPGDLTDGDVH
                     QLLVAISREHRWMHIEKLQEFDGELGFTRDQGEN"
     gene            complement(17088..17258)
                     /locus_tag="VF195_01755"
     CDS             complement(17088..17258)
                     /locus_tag="VF195_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579586.1"
                     /translation="MITGDMAREQIRDRVREAESFRRSKTTRTAQQHDRRAVARKVYS
                     GLVAALATPFRH"
     gene            17399..18277
                     /locus_tag="VF195_01760"
     CDS             17399..18277
                     /locus_tag="VF195_01760"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6579587.1"
                     /translation="MTTVAVIGLGAMGARFAQRFLDAGHEVIVWNRTPEKGEDLVSRG
                     ATPAASPAEAARSAEGVVTVVADPTALRAVTEGPDGIAAGAGGGTTVIEMSTVGSDAI
                     RWLRSALPPGVGLLDAPVLGSLSEAESGTLLVFVGGPPRLAERWTPLFGTLGSVIVAG
                     PLGSGAAAKLVANATLVGTLTLLGEAIALADRLGLGRRLTMDILASTPLGPASKRRRE
                     QLESGDFPLRFRLALARKDAELIQEAAEAAHADTRVLGAAATWFADAEEAGLGDRDYS
                     EILRLITREMSASGPD"
     gene            18280..18711
                     /locus_tag="VF195_01765"
                     /pseudo
     CDS             18280..18711
                     /locus_tag="VF195_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018959703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
     gene            18956..19031
                     /locus_tag="VF195_01770"
     tRNA            18956..19031
                     /locus_tag="VF195_01770"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18990..18992,aa:Glu,seq:ctc)
     gene            19204..19695
                     /locus_tag="VF195_01775"
     CDS             19204..19695
                     /locus_tag="VF195_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579588.1"
                     /translation="MSKETILSEPRQRSVEEAFVAMAPRLWRALLLYAGDRDVADDAV
                     SEAFTQAMSASTSIRDVRGWVWTAAFRIAAGEMRRRAEQSDDLAIDLPVPEGIDPSSL
                     IDLVRALEELTDMQRRAVILRHYMGYSNVEIARILGSSPSSVGVRLFRSTRRLRQLLN
                     EEG"
     gene            19697..20287
                     /locus_tag="VF195_01780"
     CDS             19697..20287
                     /locus_tag="VF195_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579589.1"
                     /translation="MTTSLKDRFRQVDAIPTPWDERAAAVPTQPPQAASHWAPRALVL
                     GAAAVAVAFVAIVAGPAVEQRPAPRGDASWLVGAQQSCVEQYSVEALANREYAFEGVI
                     GDVAGPADPESPDPAHLTTTVTFEVERWFWGGSDAEIALQTYAATSSVGELEDSVGAR
                     LLVSGDDIYIWACGFTQPASEQARSDYGAAAAERSA"
     gene            20287..20736
                     /locus_tag="VF195_01785"
     CDS             20287..20736
                     /locus_tag="VF195_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579590.1"
                     /translation="MRRLIATILTISFALLTGHPATAVPPRVSVSAELRCLEGGGIGI
                     DLTIENTGRRIARIDPDFHLLVQTIRKGRQPGVVLFVFPAPGFDRIPPGESRTFLLTA
                     GEPFEGEPAIDFSGPRIILETEVWLRGRDNPVVRTLTYPGCAPIGRQ"
     gene            21168..21605
                     /locus_tag="VF195_01790"
     CDS             21168..21605
                     /locus_tag="VF195_01790"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6579591.1"
                     /translation="MHTKPLVVAAVAAFALLTVACGDGSDVGSGTEQDPRTIEVTALD
                     DPAFDPSTIEVAAGETVRFVVTNTGEADHEFVVGDMEMQEMAEEQAMEGMHGHAQAMA
                     ALALEPDATAETVLTFEEAGELFYGCHVDGHYEAGMVGTITVT"
     gene            complement(21598..24045)
                     /locus_tag="VF195_01795"
     CDS             complement(21598..24045)
                     /locus_tag="VF195_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014211201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6579592.1"
                     /translation="MTTVVLHTGGLNWASEKAIVEAVLGRRRGVEAVEANPVAQTATV
                     TYDPKRTSVAELRRWVEECGLHCAGQSVPTHVCDPLAEPDLSHAHTMHQGATVATTEA
                     RHDGHAIPVEHEAHEMAAGEGLPSPQEAMGHGGHAGMSMDAMVRDMRNRFLVAAFLSV
                     PILLWSPIGRDVLGFDVTAPLGLRDDVFSLILSLPVIFYSGWIFFDGAVRALRARTLD
                     MMVLVAVAIGVGWLYSVGVTLTGGGEVFYEAATVLATFVLLGHWFEMRARGGANDAIR
                     TLLDLAPPMALLIRDGEPVEVPTAEVAVGDLLLVRPGAKIAVDGVVEDGDSEVDESVV
                     TGESLPVHKEPGSEVIGATINENGTLRVRATKIGADTALAQIVKLVQEAQNSKAPGQK
                     LADRAAFWLVFVALIGGAGTFLVWLAAGRSVDEAMLFAITVVVITCPDALGLATPTAI
                     MVGTGLGATRGILFKYAIALEEAASIGVVVMDKTGTLTRGEPEVSDVIADALSERELL
                     RLVAAVERESEHPLAEAVVDHADANDVPTARAEAFENIPGHGATANVDGHQVAVGNRR
                     LMEREGIDMGSLEERRKELAASGKTAVIAAVDGRAAGLIGIADAARPTSRAAVGALAE
                     SGVGVVMLTGDNEATARRIAGELGVSTVIAEVLPGDKAGKIAELQREGRKVAMVGDGV
                     NDAPALAQADLGIAIGAGTDVAIETADVVLMRSDPLDVPTALRIGRGTLRKMRQNLGW
                     AIGYNAIALPIAAGVFEPAFGLTLRPEIAALSMSGSSLIVAVNALWLKRLRLPRGDSD
                     TETEPRGRAAGVHPGIT"
     gene            complement(24158..26662)
                     /locus_tag="VF195_01800"
     CDS             complement(24158..26662)
                     /locus_tag="VF195_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6579593.1"
                     /translation="MEPGGEAAGRVALALERGGGVACAACLRTIESELESLPGVRSVQ
                     TDVVNERASVEFDPSAVSVEDMRHAVASIGYRVQQPPRAASDGTEDVEAAARRAEVRD
                     LTWRVVVGAALTAPVLISVMASEIFDAAWVPEFLMNRWIQLALTIPVFLYTGWPIHTV
                     GWRTLLHRTAEMNTLITVGTTAAFAYSVLVTVAPGLFPAEVREVYFEAVGVILTLILL
                     GRLFEARAKAGTGEAIRKLIGLRPQTARVVRDGQEHETPVDDVQVGDVVAVRPGEKIP
                     VDGEIVDGRSSIDESMVTGEPIPVTKGPDEAVIGATINQTGAFHFRATKVGKDTMLAQ
                     IIELVEQAQASKAPIQRLADLVASYFVPVVIFIAIGTFVVWFVLGPSPALTLGLVSAV
                     SVLIIACPCALGLATPLSIMVGTGKGAEHGVLIRSAEALETAHKLHTIVLDKTGTITK
                     GEPSLTDIVPIDSASEDDLVRLVASAERTSEHPLGQAIVRGAEERGIALVEPTDFQSV
                     TGRGIEATVDERTVLIGTRQLLADRGIDAASLVADGERLAGQGKTPVYAAVDGAPAGV
                     VAVADTLKEDSPRAIRALRELGLEVVMITGDNRRTAEAVAREVGIARVLAEVLPEDKA
                     LEVRRLQDEGKLVAMVGDGINDAPALAQADVGVAIGTGTDVAIEAADVTLISGALEGV
                     VTSVGLSRSTMRNIRQNLFFAFVYNILGIPVAAGALYAATGVLLSPIIAAAAMALSSL
                     SVVTNANRLRSFRASELPTKGDTADRVSVEVHEHQERPEDAKEEAMATVKDPVCGMDI
                     DPATAAASEEYEGTTYYFCSENCHQQFVATPERFVA"
BASE COUNT         4230 a         9418 c         9023 g         4013 t
ORIGIN      
        1 caacggatgc ccgtgcttcg acacgaagac ggggtcgacg acgatgttcg ggagcgacat
       61 ctccgcggcc gactccgcca cggcctcgac gatggcggcc gaggcgagca tccccgtctt
      121 cgccgcatcg acaccgatgt ctccggccac ggcgcgtatc tgctcggcca cggtcgtggg
      181 tgagagctcc tcgaagcctg cgacgccctt cgtgttctgg atggtgaccg ccgtgatcgc
      241 ggtcatgccg aacacgccga gcgcgctgaa cgtcttgaga tcggcctgga tccctgcgcc
      301 accaccggag tcggacccgg cgatcgtcag cgcccgcgct cgaggaggtc cccctcgcgg
      361 ggaaggtcca tcgttcatcc gaagtgatcc cacccttcga tctcgagggg gcgttccgtt
      421 ccatcctcgt ccacggccac gagtcctcca gcgctcctgg cagcgctcct ggcgcccccg
      481 ccttcgatga tgacgccgat atccctgagc gacacgccga acgcctcatg gacgtctctc
      541 gccgctccgg cgaccgcatc ggggccggga agggtcgcga tcagctcgta gtcctctccc
      601 ccgccgagcg cctcgtgcac ggtcgacgct gggtgcaccg gcacgtgcgc gacctcgacc
      661 cgtgccccga caccgctcgc ccggcacagc cgcgacagat cgatggtcag gccgtcggag
      721 acatccatca tcgcggtggc accgtgggca gacaggatcg aggcctcgcc gacgcgcggg
      781 acagggcgca tcgagcggcg gagcgcgtcg cgctcgtcgt cggaccagga gcgttgcgag
      841 gagacccgca gccccgcggc cgagccgccg agtgaccccg tcacgaccac ccggtcgccg
      901 gaacgggcgc cgtcccggcg cactgcgcgc cccggggcca cctccccggt cacggtgacg
      961 acgatcgcga gctcctcgcc gcgcgagagg ttcccgccca ccagcgacac cgcgaactcg
     1021 tcgcagcact cgcgcatccc ggcggccagc tcgacgaccc acgacgcgtc gatcgcgtcg
     1081 gtgagggtca gcgcgcagag cgcgaaccgc gggctacccg ccatcgcggc gatgtcgctc
     1141 acgttcacgg cgatcgcctt gtagccgaga tcgcgcggtg tggtccgatc gatgaggaag
     1201 tgcacccctt cgaccatggc gtccgtcgtc aggacaacct ctcccgaacc accccggacg
     1261 accgcggcat cgtcgcccgg tcccacgagg acctcgggcc ccgcgccgga cagcaccctg
     1321 ccgatcgcgg cgatcagttc gtcctcggtg aggtccatct gctcggctct ggccgttccg
     1381 cttggccgtt tccggtctgg aggaggcagc ttgggcgttc gcttagactc taggctttcc
     1441 gcttgtccac gcgtttcgcg tactcaccga cccagccgag gatcagccga ggatcgatca
     1501 gccgaggatc aggaggaccg agtgtcagac gtgttcgccg atccgactcc ggaggagctc
     1561 cgtcgcttca ccgaagcgat gccggaatgt cggatcaccg agttcggcaa catgaacgtc
     1621 cagacccgag tggtgtcccg cagcgccgac agcaccttcg tcgtcgatcg accgtccagc
     1681 ggcaagacga tgacccgcga cgcctatgac aagatcgccc gccgacagga cgcgtacctg
     1741 gctgatcacg acgtgatccg gatcgacggt tggatcggga acgacgccga gctccggacc
     1801 cgagcccgcc tgctcatcga gaagcgctac gcgaacatcg ccgggatgca gcagaagctg
     1861 tacttccctc gcgacgacga cgccgatccg gacgtccagg tgatctacac ccccggcctg
     1921 accgcgcccg ggtatcccgg tgaccgggtg atcgcggtcg acctcgacgc gaacgtcacg
     1981 cgcgtcctca actccgacta cttcggtgag tccaagaagg gcgggctgcg gatgtggaac
     2041 aacatcgtgt acgagaaggg tggcctcgct ctgcacgccg ggctgaaggt gatcccgacg
     2101 tcggccggcg acaaggtgtt catgatcatc ggtctgtccg gaacggggaa gacgacgacg
     2161 acgttcacga cgcagaacgg ctcgaagccc atccaggacg acttcgtggg gctgatgcct
     2221 ggaggtcgtg cgttcggcac ggagaacggc tgcttcgcca agacgttctc gctcgatccg
     2281 gagttcgagc cgtcgatcta tggcgctgtc accaaaccaa ccacctacct cgagaacgcg
     2341 taccaggacg actccggtac ggtgaacttc ttcgaggaga gctacacgca gaacggtcgg
     2401 gccgtcttcg agatgcgaga cctgcagacc ttcgaggacg cccggaatgt cggccccgtc
     2461 gactacctgc tgatcctcaa ccggaacgag aacatcatcc cgtccgtcgc gaagctcgac
     2521 caggagcagg ctgccgcgta cttcatgctc ggagagacga cggggacatc ggctggcggc
     2581 gccgccgagg agggcaagtt ccttcgcgtc ccgggcacca accccttctt cccgctcccg
     2641 cacggcctgc agggaaaccg cctgctcgag ttgctcgcga cccatcccat cgagacctac
     2701 ctgttgaaca ccggcagggt cggcgggaaa gacggtgacg accgctcgaa gaaggtgaag
     2761 atcccgcaca cgtcggcgtg cgtgaagggg atcgccgagc agaccatcgt gttcgaggag
     2821 gatccggact tccggtacca ggtggcctcc gagatcccgg ggttcgacga cccggaactc
     2881 cttcgcccgg ccctcctcta cgagcgggag ggccgcatcg aggagtaccg cgcgatcgtt
     2941 gagcggctga aggtggagcg ggtcgaacac ctgcaacggt tcacggagct ctccgaggac
     3001 atcatcaagg ccgctggctg acgccccacg gtcctgccgt acccccctct cctagctggt
     3061 caaggcgacc agtcagcact ttgacgctcc tacccccccg gggtatatgt tccgaagcca
     3121 gcgacgggag gatgtccagg gatggggcac gagggtcacg gcgggcacga gggacacggc
     3181 gggcacgagg gtcacggcgg gcacgagggt cacgcggcga cgttccgcga tcggttctgg
     3241 ctcagcctcg ccctgagcgt cccggtcttg ctgttctccg agatgatcca ggactggctc
     3301 gggttcagcc tcccaacgtt ccccggcgac gacctcgtgg caccgatcct cggctccgcg
     3361 gtcttcctct acggcggcca acccttcctc acgggcgccc tggacgaagc acgggcacgg
     3421 cgccccggca tgatgttgct catcgcgctc gcgctcatcg tggcgttcgg tgccagcgtg
     3481 gccagcgccg tcggatggct cgacctcgag ttctggtggg agctcgcgct cctcatcgac
     3541 gtgatgctgc tgggtcactg gctggagatg cgtgctctcg gccaggcgac gagcgcgctc
     3601 gaggctctcg cctcgctcct gcccgatacg gccgagctcg tgaccgacag cggcaccgag
     3661 gaggtaacgc tcgacgccct ccgtcccggc gacgtcctgc tcgtccgctc gggggcccgg
     3721 gtccctgccg atggcacgat cgtcgagggt gaggcggcgt tcgacgagtc gatgatcacc
     3781 ggcgagtccc gtcccgtgac gcggggcccg ggcgaccgga tcgtgggagg caccatcgcg
     3841 accgactcgt cggtccgcgt cagggtgacc gcggtgggtg aggagacgac gctggcgggg
     3901 atccgccgac tggtcgcgga ggcgcaggcg tcacggtccc gggcacaggc gctcgccgac
     3961 cgggcggcgg cgctcctgtt ctacgtggcc ctcgcgtcgg ggacgatcac gctgatcgcg
     4021 tggctcgcag ccggtgagcc cgaccaggcg atggaacgca cggtcacggt gctcgtgatc
     4081 gcgtgtcccc acgcgctcgg cctcgcgatc ccgctcgtgg tcgcgatctc cacggcgatg
     4141 tccgcgcgcg acgggatcct cgtgaaggac cgcctcgccc tcgagcggat gcgcaccgtc
     4201 gacaccgtgc tcttcgacaa gacgggaacg ctgaccacgg gaacgcccgc cgtccgcgag
     4261 gtcctcgccg actccggcga cgaagacacc gcgctatcgc tcgcggcggc cgcagaggcc
     4321 gacagcgagc atccactcgc gcgcgcgatc gtggaggaag cgcgccggcg gcggctcgac
     4381 gttccgtccg cgtcgggctt ccggtcgatc accgggcgtg ggatccgcgc ggacgtgctg
     4441 gggtccgaga tcgcggtggg aggccctgca ttgcttcggg agctgggggt ggagatgccg
     4501 gagagatcct cggatacggt cgccgactgg cggcgccgtg gttcctcgat cctcaccgtg
     4561 gtgcgggacg ggaaacccgt tgcgatgatc gcgctggagg acacggtgcg ccccgaatcc
     4621 cggcaggcga tcgccgacct ccggaacgcg ggactcgagg ttctgatgat caccggtgat
     4681 gcacgtgagg tcgcggaggc gatcgggcgg cgcgacctgg ggatcgacga cgtccgcgcg
     4741 gaggtgatgc cggagcacaa ggaggccgtc ggcggcgggc gtcctcgctc cctggggcgt
     4801 ggtgctcgcc ccggcgctgg ggccgtggcg atgagcctct cgacggtcgt ggtcgcgttc
     4861 aacgctctgc tgctcaggcg gctcgatctc cgtccggggg aggcccaggc ccccctcgag
     4921 accgccccag cagatacccg gtgacgaact ggagcgagat cgggacgagg aggacgacga
     4981 tgtaccagtt gagctccgcg gcgtcgtcgg tgaacgcgcg tccctgggtc tcgacgctcg
     5041 cgaagagcag gaagaggccc atcaggccga accacacgcc ggcgcgcgcc atcacgaaga
     5101 gggcgtagag gacgagatcg cgtcggccca cgaggtcgtc gatcgggtgt cggagccagt
     5161 gcacgaccga ccgaacgccg agggtcgcga acagggccac cgccaccagc tcgacgtcgc
     5221 tcatccgtgc tgctcccgga ggacacggag gacctctccg tcggtgacct gatcgaagtc
     5281 ccggtaccac tcccccacgg caaggaaccc gaacggctcc cggaggagca ccacgtcgtc
     5341 ggcctccgcc tcgaggctgg cacgcgcggc cggcggaccg acgggcgcgg cgaacacaac
     5401 ccgactggcg ccggccgcgc gcgcccaccg cagcgcggcg gtggcggtgg cgccggtcgc
     5461 gacgccgtca tcgacgacca ccaccgtccg tccggcgacc ggcgccggcg gacggccctc
     5521 tcgatacgcg gcttcccggc gcgcgatctc ggtctcctgg agggccgcct cccgctggat
     5581 gtactccatc gagaccccca gctgccgcac cgcccgctcg tgcagcacgc gcactccggg
     5641 agccaccgcc ccgatcgcga gctcgggatt gccgggtgcc ccgagcttcc gggggatcac
     5701 cacgtccaat ggcgctccca ggttccgcgc cacgggaccg gcgaccgtca caccgccccg
     5761 ggggatcgcg agcaccgtcg cgggctccgc cacgaccctc gcgaccgccg cggccagcgc
     5821 ctcgcccgcc tcctcccgat cgcggaacac tctctcgatc atcgcaccgg cgttgctcgg
     5881 tccgcccacc cacgactagc atggcgcgca tccccatgga cccgaggagg cacctggtgg
     5941 ttcacgcgta catcctgatc cagacggagg tgggcaaagc cgcgtccgtc gccaacgccg
     6001 tgcggaacac cacaggggtc accgaagccg aggacgtgac gggtccctac gacgtcatcg
     6061 tgcgcgccga ggcggacagc gtggacgacc tgggcaagct ggtggtcgca cgcatccagg
     6121 cggtcgaggg catcacccgg accctcacgt gcccggtggt ccacctctga cgaggcaccg
     6181 ccgggcggac gccgcccccg cctcggctca gaggcccaga gcccgcacac cggcgctctt
     6241 gcgctccgcc tcatgccgtt cgacggcgag atcgagtagg cgctccacga ggtcaccgta
     6301 ggacaagccc gatgcctccc agagcttggg atacatcgag atcgacgtga accccgggat
     6361 cgtgttgagc tcgttgagcc agagctcgtc cgtgccgtgc aggaagaagt ccacccgcgc
     6421 catcccggcg cacttgagcg ctccgaacgc ggccacggcg aggcgctgca cgtcctccaa
     6481 gacgcggggc tcgaggtccg cggggatcag gagccgcgcc ccgtgctcgt cgaggtactt
     6541 cgcctcgtag tcgtagaact cgtggtcctg cggaacgatc tcgcccgcga cggaagcgac
     6601 cggatcgtcg ttgccgagga cggcgcattc gatctcccgg gcgccctcgg ccgccttctc
     6661 gacgagcgcg tccctcgcga accggaacgc ctcctcgaga cccgcggcga gctcctcgtt
     6721 gtcgtgcacc ttggacacgc ccacggagga accgagggtc gccggtttcg tgaagagggg
     6781 gtacccgagc gcctccgcgg ccgcctcgat cgtctcggga tcgtcgaccc agccggactc
     6841 ccgcaccgcc tcgtagggac cgaccgggag ccccgccgcg acgaacagcc gcttctgcac
     6901 atccttatcc atcccgagag cggaggcgag gacgcccgca ccgacgtatg ggacgccggc
     6961 gagctcgagg aggccctgca ccgtgccgtc ctcgccgtag ggcccgtgga gcacggggaa
     7021 gacgacgtcg atcggctccc tcgatccgtc ggctccgacg atctccgccg acatcccctc
     7081 ggccgcgagc tccaccgaac cgccgctgcc gtccgtgacc tccggcatgc ggccggtctc
     7141 ggacgggagg gccggcgggc ccgcgacgcg atgccagcgg ccctcacgag tgatcccgat
     7201 cgggatcacc tcggtccgct ccggatcgag cgcatcgatc accgatcgag ccgacaggca
     7261 cgacacctcg tgctcggccg accggccgcc gaacaggacg gcgacgcgcc ggcgcttcat
     7321 gccttgtcga gggcccgggt gaagtcctcg atcagatcgt cggcgtcctc gagcccgacg
     7381 gccacccgca agagcccgtc ggcgatcccc gccgaccgcc gtgcgtcggg atcgacctgt
     7441 cggtgggtgg tggaagcagg gtgcgcgacc agggtcatca cgctccccag gctcccgccg
     7501 atccagccga gctccagcgc ctccgcgacc cggctgcctg cctccacgcc tcccgccacc
     7561 tcgaaggtca gcgtgccccc gaacccgcgc agctggcggg cggcgatctc gtgttgcgga
     7621 tgcgagacga gccccggata gtggacacgc tcgacggagg gatgggcgtc gagcgcctcg
     7681 gccaaccttc ccgcggacgc acaatggtgg tccagccgca ggccgagggt cgaaaggccg
     7741 cgcaaacaga gccaagcctc gagcggctgc atgctgcccc ccaggtcgat gacggtggcg
     7801 cgcagcctcc ggaagaggtc ctcgttcgtg cacgcgaccc cgccgatgag atccgcatgc
     7861 ccgccgatgt acttcgtcgc ggagtgcagc accacgtcga aacccaggct gatcggagtg
     7921 cagaggtagg gcgacgcgaa cgtgttgtcg acggcggaca ggacccccga gtcccggcac
     7981 agctcggcga gcaccgccag atccgggacc gtggtcgtgg gattcacgat cgtctcgcag
     8041 tagaagagat cggcgccggg gagcgcatca cggatctggt cgacatccgt ggggtcgacg
     8101 aacgtcgtct cgatcccgaa gcgagggagg acgtgctgca gcatcgacac ggtgccgccg
     8161 tagagcgccg tgcccgccac gatccgcgaa cccgcgctcg cgacggtcag gaggagcgaa
     8221 gagatggccg ccatgccgga tgcgaatccg atcgcggact ccgcgtcttc gagatcggcc
     8281 atcgcggact ggaacgcgtc gacggtaggg ttcccgtacc cgcgcgagta cacgtagccc
     8341 ggaccgtcga agcggatcgc gcgcgcgaac tcctcgttcg tctcgaaccg gaacgtcgag
     8401 gactggaaga tgggcacgct gctcgacgac tgctcgggca gcgccggtcg atgggggtgc
     8461 acggcgcgcg tctggaatcg gcgccgcttc cccgggtcgc tcatggcggg cagcctactt
     8521 cgtccatcgg cgcgggtagc ggtcacgtag gtcgacggcg agccgggtga tcgcgtccat
     8581 cacctccccg gtgagcgcac gcagctccac ctgccccccg tccggttcgg cggggagcgg
     8641 gatcggctcg ccgaccgtgg tccagatcgg tcggccgaac ctcagggatc cccggcccga
     8701 cttctgccag acggcgcgcg atccccagct ggccatcggg atgatcggca cgccgctcgc
     8761 gagcgccact cggacgaggc cggtcttgcc ttccatcggc agtccgtcgt ctcgcttcgt
     8821 gaccgtcccc tcggggtaga cgacgaccac ctccccacga tcgagcgctg cgacagcgag
     8881 gtcgagcgag gatcggtcgc gcgtgccccg agtgaccggg atctggccgg ccccgcgcag
     8941 cgcacgcccc acgaggggga tcttgaacag gtcccccttc gcgaggaacc gcgggcggcg
     9001 tcgggcacgc acgatcgcgt caccgttcgt cagaggatcc aggtaggaga tgtggttgca
     9061 cgcgacgatc gccggtccat tcctggggat gcgatccagg tgctcatacg acagacggaa
     9121 ccagaaccga agcggcggga gcgccacggc cttggcgacg tgataccagg gttccgttgc
     9181 cgtccctcct cgctcgggcg ccgtgagggt acagcgaggg ctcgctaggc tgacgagctc
     9241 gtgcgcgcga tcgccctccc cgtgaagtcc cttggccgcg cgaagtcccg gctgtcctct
     9301 ctcctcgccc cgctcgagcg gggcgcgctc gccctggcga tgttcgagga cgtcttggac
     9361 gccaccctgg ccgtcgacgg ctgggagacg tgggtcgtct cctccgacga gagcgtcctg
     9421 gagatcgccc tcgctcgggg ggcgcacggg atctcggagc tccgaccgac gctccgctcc
     9481 gcgacgctcc agatcgaggc ggaggctgcg gcccgatcca ccgaggcgct cgcgatcctc
     9541 ctggccgaca cgccgctcgt gaccctcgag gcactgcgag cggcgctgca cacgctcggt
     9601 cccgtggtga tcgccccgtc ctcggacgaa cgtgggacga acctcctgct ccgccgtccg
     9661 ccaggagcca tccccaaccg cttcggtccg gattcgttcc gacgacacct ccaaacggcc
     9721 gccgaacgcg gcctgccgac ggccgtcgtc gagcgggagg agctgtcctt cgacgtggac
     9781 gagccgggcg atatcctcac ggtgctcgag acccggcgcc cggggcggac gctcgacgtg
     9841 tgccgggaca tggatctcgc cggcaggctg gcggcacgga cctgacagga gcgagcgtgg
     9901 atcaggggac gatcaaggac ttcgacgagg agggccgcac cggcgtgctg ctcaccgacg
     9961 agcgcgcgga ggtcgtgatc gacacggact cgctcgatgc ctcggggatc cgcaccctgc
    10021 ggatcggtca gcgggtgaag ttcgagacgg aggagcgaga cggggcgacg gtcgctcgtt
    10081 cactgcgcat cgtgacctgg gactgaccgc gccgtcctag ccggagttgt tgcccaggac
    10141 ctggtggcgg aactccttgc ccggcgtgaa cgccggcagg tcgcgctccg ggacgacgat
    10201 gggaacgtcg ggctgccgag gattgcgggc gatccgacgg ttgcgatgtt tccgctcgaa
    10261 cgttccgaag tccgcgagag tcacgcgatc accttccacc accgtttcgc ggaccgtgtt
    10321 gatcgccgcg tcgatcacgc ggccgacctc ggccttgctc aggccggtct tcttcgatac
    10381 ctcggtgacc aagccgccct tgttcatgat gctccctcca ggtcgtcgcg gttgcattcg
    10441 aacgtaccga acgtgggccg gaggggtcaa ccgagcgagc gtgcacggga agcattccga
    10501 tcctcggtcc ggtgttgtgc cggcctcgga ggccgcccgg aagtcctccg gtagacttgc
    10561 gagaaccctg gggcgtggtg taacggcagc acgacaggtt ctggccctgt tagtcgaggt
    10621 tcgaatcctt gcgccccagc tcgcgagggg aggacctatc ccggttcgag ccgggttcta
    10681 gagacccagg ccgaccagga ggccgacgag caccaccgcc aggctcatcc cgaacagcgc
    10741 gagggtctgc ccgacctcgc ttcgtaagtc gtcgtcgtca tcacgagact gcatgcgttc
    10801 ttcgtacccc gatgtccgcc gctccatacg cgaagatgtc ggaacatcgg cgcagaactt
    10861 gaacgtcgac cctcaggcca cacggcgggg gcgagcggac gcaggttcca ggaggtcgag
    10921 cacgcgcgat tccacagccg cccgaacgcg gtcatctgtc agatcccggc tccgcggcag
    10981 cgcgatcgtc tcctcgttca ggaaccgatc gatgatgcgc ggatcgatgt acgacgcccg
    11041 gcacaccgcc ggggtgttcc cgagcgcctc ggccacctcc ttcaccaccg aggacaccac
    11101 ccgtttgcgc gacgtcacgg acctgagtcc ttcctcctcg gaggcgagga gagcggcggt
    11161 gagcacggtg gcgtgccagg tccggaagtc cttggccgag atgtcctcac gtgcgaggtc
    11221 cttgaggtag ccgttgatgt cttccgaccg gacgtcccgc cactcgggtc catcacggaa
    11281 cgcaaggaac tccttccctc cgcccttccg tcgcaggagc gcacgcgtca gacgggcgag
    11341 cgcctgatcc tcgatcgtct ggatccggcg ctggccgcct ttcgcagaga agtcgaatcg
    11401 cacctcgtcg cctcgcacgc gcacgtgatc ccggcggatg gtcgccagac cgtacgagcc
    11461 attcacctcc gtgtagctct ccgatccgat cctgaagaac ccgagatcga ggagccggac
    11521 ggcacccgcc agcgcgcgtt ggcgtgtgag ccccggtgac acgatgtctc gctcgcagat
    11581 ccgccgcatg gcggggagtc gtcgagccaa gcggagaacg cgatcgaact tctgccgatc
    11641 cctccgggcg cgccaatcct cgtggtacag gtactgccga cgtcccgccg catcggtgcc
    11701 gaccgcctgg aggtgccccc ggtcgtccgg acagatccag acgtcctccc aggccggcgg
    11761 gatggcaagc gcgcggatcc gctcgcgcgt ggcctcgtcc cgtaccggct gcccgttggg
    11821 gtgcaggtac gtgaagccac gaccctgccg ccgtcgggtg atccccggag agctccagcg
    11881 cacgcgtttc agcgcgctca tcgccgatcg tccccgctcg ggtccacgaa cgtgacgtcg
    11941 acccgctcct cgggacgcgc ccgctgctcc tcggcctggc ccgcgagcac ctcgtggtcc
    12001 ttcgtgccga gaggcggagg ggcgagcgag cgcggccaga gatgacgcgc cagcacgacg
    12061 ttcacctcac cggccaccag gaagagctgc gcggccaggt agatccatgc gagcagaccg
    12121 atcacggttg cgaagagccc gtagacggcg gacgcatgac cgatctggcg atcgacgatc
    12181 catctcccga ccaggagcag cgcggtccat ccgaccgcgg cgacgaccgc gccggggagg
    12241 acctgacgcc acgcgacgct cgcgacggtc aggacccggt acgcgaatgc gaacacggcg
    12301 atagacacga gggtcagccc gacgagcgag atcgcgcgca ggacgacgga cccccacccg
    12361 gtctccgtgc ccgtcccggc gaggaaggtc gaaaggacga tcgatgcggc gaagacgaac
    12421 aacaggagga acgcgcggac gatccggcca aggagtccgg gccgctccgc gcgtggcacg
    12481 tcccagatgt catccatcgc ctgctgcgcc gcggccacga ccgccgtccc cgtccagagg
    12541 gctccgacca gtccgacggc cagcgcgagc ggtgaactag agagctgctc cacgctcctc
    12601 tcgatgactc ccccgatcga cgtcccgacc accgggaact gctcgaggat cgaatcgatc
    12661 aggcgcttct ggagatcggg gttgccgtcc agcacgaagg ccgcgaccgt ggcgaagacc
    12721 aacaggagcg ggaagagaga gaagaatccg tagtaggcga tcagggccgc gagttggctc
    12781 gcccgatcat caccgaactt cttcacgacg gcgaagggga acgccagcgc acggtgcgct
    12841 cgctgaacct cgtcggcgcg atgcagcaac cgctgcgcgg ccccctcttt cggctccgtc
    12901 atgccgggtt ccttccctgg ccccgatggg gtcaaacgcc gaggtcggga aggatgcgag
    12961 ctcgatagcc tgtcggcatg agcgaatccg ggaccgtggg atccggcggc ctgtgccccg
    13021 tatgcggcgt cggcacgctg gccgacatcg acttcggcgg cgacgagaac gtccaggaca
    13081 ccgagagccg ccaggtggac acctacacct gcgggcacga gatcgagcgc gcacccctcg
    13141 agacggcgga cgcggaacga ctggacgtcg agcagcggac ctcggaggaa acgacgtcgc
    13201 ccacgccgga ggaagggacc gcgccctagg ggtctcggga ccgggtgccg agaggcatcg
    13261 cggggttttc cgagggagac ggctccgccg acggcgacgg ggacggctgg gcggatgggg
    13321 acggctcgga cgacggcgag ggtgacggct cttccgatgg ggacggcgac ggcgacggcg
    13381 tggccgacgg cacggcggga acggccacgg gcgacggctc cggcgggggc ggatcggcgt
    13441 agacggcata gctcgccacc gggaatccca tgaccgccat cccctcggtc gcctcggtca
    13501 tgaaatcgtg ccagatcttg gctgggatcg ttccgccgta cacctccggg accccggcga
    13561 cgtcgcgcat cggccgggct ccatcgggat agcccaccca gacacacgtc acgagctggg
    13621 gcacgtagcc gcagaaccac gcatccgtcg cgtcctgggc ggttcccgtc ttgccggcgg
    13681 cggggcgatc gatcaggcgg gcggccgtcc ccgtcccgtg atcgacgact tcctgcagga
    13741 cccgtgtggt cgcgtcggca tcctgcgggg acaggacctc gctgcccacg ctggcgagcc
    13801 cgcggagcgg cccctggaga acctccccgt cggaaccggt gacccggtcg atcgccgtcg
    13861 gctccacgta ggagcctcgc gcagcgagcg tggcgtaggc cgacgtcatc tccagcggcg
    13921 tgacctccga cgttccgaga ccgatcgagc acaccggagc caaccgggat cggatgccca
    13981 tccggtgggc gacctcggcg acggcctcgg gcccaacctc gaccgtgagt tgcgcgaaga
    14041 tcgtgttcac cgagccggcc gtcgcgttct cgatcgacat cgtcccggcg gattggtcgc
    14101 ccgcgttcga cagactccac ggccgcccgc ccgtctcgca gaacggatcc gggatcgtga
    14161 tcgacgaggg accggcccgg acttcgagcg gggagatccc gcgctcgagt gccgcgacga
    14221 ggacgaacgg cttgaacgcg ctgcccgcct gtcgccgggc ctgcgtggcg aggttgaact
    14281 cgctccgcgc gaagctcctg ccgcccacca tcgcgcggat cgcaccgttc ctcggatcga
    14341 tcgcaacgag cgccgcctcg gggtcggacc gggacgggag ggagatgccg atcgcgcgct
    14401 cggcggctcg ctgccactcc atgtcgaggg tggttcggac gcggagcccg cctcggtaga
    14461 gagcgtccag tccgtagccg gccttcaggt ccaatcgcac gtgctccatg agatgcggcg
    14521 cccccgagcg cacgttcgct tcctcggcga ggccgagggg ccgagcgcgc agcagctcag
    14581 cacgcgcgag accgatcgac ccgacctccg ccatcctcgt cagggcgagg tcacgggcga
    14641 ggcgggcctc ctccgggtgc tcgaacggat ccctcgccga cggtgccgag atcagaccgg
    14701 cgagcgtcgc cgactgcagg agcgtgaggt ccttcgcatc acgatcgaag tacgtgcgag
    14761 ccgcagcctg gacgccgtac gcgccgtgtc cgaagtagac ggtgttcaga tacccgcgca
    14821 ggatctcgtg cttggaggtc gtgtcctcga gcttgacggc gaggatcgcc tcccgcacct
    14881 tgcgcgacac cgatcgctcc ggccccgtca gcacgttctt cacgtattgc tgcgtgatcg
    14941 tcgaaccacc ctgggtcagc gacccgctgg tgaggttcct ccacgtcgcg cgaaccaagg
    15001 ccagcttgtc gataccgtcg tgccggtaga agtccgcgtc ctcgaccgcc accacggcct
    15061 cgcgcaacga ctccggcatc tgcgagaagg ggatcaagac ccggtcgacg cccgcgtgca
    15121 gctccgcgac ctcgttgccg ttgcgatcga gcagcaccgt cgtctccgcg agtggcggga
    15181 tctcggggag cgatgtcgtc gcgtacagcg cgacgagagc ccccgtggcg gagaccaacg
    15241 ggaggaatat cgagaggatc gcgatccagg ccaccaggcg ccctcggctc atgggcaggg
    15301 acggcgcggt cgcgacccat tcgtcgtcac gggcggggcg cgatcggatg gtgcggggcg
    15361 aggtggcgcg ggcggcactc atgggggccg caaggaacta cccagatgac ttgggccgca
    15421 aacggctgag tcgttcagct cccccacgcc gcacgcgctg tcggcgatga gggaggacag
    15481 acgtctcgcc gatagcatgg ctctctatcg gaggaggcgc tcgtggacac atcgacacag
    15541 aggatccccg tcaccctcgt ccccggcgac ggcatcggac gcgaagtcac ccaggccgcc
    15601 aggcggatcg tcgaggcgac cgacgccccg atcgactggg aagagaccga ggccggagcc
    15661 gaggtgttca agaagggtct gccctcgggc gtcccagagg agacgatcgc atctgtctcg
    15721 cgtacgcgcg tgatgttgaa agggccgctc gagacccccg tgggcttcgg ggagaagagc
    15781 gcgaacgtca cgctccggaa gctgttcgag acctacgcga acgtccggcc ggtccgtgag
    15841 ctcccgggcg tgaagactcc ctacagcggc cgtggcatcg atctcgtcat ggtccgggag
    15901 aacgtcgagg acctctacgc cggcatcgag tacatgcaga ccccgggcgt cgcggaagcg
    15961 ctcaagctga tctcggtgaa gggatgcgag aagatcgcgc ggttcgcctt cgagtacgca
    16021 cggagcgagc accgcaccct ggtcacgtgc gcgaccaagg cgaacatcat gaagctgtcc
    16081 gaggggacgc tgaagcgggt gttcgagcag gtcgcacccg actaccccga catcgagtcg
    16141 tggcatctga tcgtcgacaa ttgcgcgcac cagctcgtga agcgcccgga acagttcgac
    16201 gtgctcgtga tgacgaacat gaacggcgac atcctgtccg acctgacgag cgccctcgtg
    16261 ggaggtctcg ggttcgctcc gagcgccaac atcgggagcg aggtggcgat cttcgaagcg
    16321 gtgcacggtt cggcgccgaa gtacgcgggc aaggacgtga tcaatcccac cgcgttgatc
    16381 ctgtccaccg tcctgatgct ccggtacctc ggcctgttcg cacaggcgga cgcgatcgag
    16441 cacgcggtcc tggtcaccct cgaggacggc gtgctcaccg gtgacgtcgt cggctacggc
    16501 aacggtgcgt ccacgacccg gtacacggag gcgatcatcg ggaacctcgg ccgcaggtcc
    16561 gaggaatgga cggtacggga gcaccggccg ctcgagctcc ccaagctctc ggacgcgatc
    16621 gactacgtcc ggcccgagtc ccgcgccgtg gtcgggatgg acgtcttcat cgaatcgccg
    16681 atgggcccga acgagctggg cgcctcgctg atggagctga cgcacgagac gccgctgtcg
    16741 ctgaagctcg tctcttcacg cgggacgaag gtgttcccgc ccaccggggc tatcaccgac
    16801 acggtggacc actaccgctg tcggttcatc atcaaggaga gccccggcga tctcaccgac
    16861 ggcgacgtgc atcagctgct cgtggccatc agccgcgagc accgctggat gcacatcgag
    16921 aagctgcagg aattcgacgg agagctcggg ttcactcggg atcagggaga gaactgagcc
    16981 ccacagagcc tgaagaacga ggaggagccg gggccgacgg ctgggccgtc gatcccggct
    17041 ctccggggtc ccgctcgttg tccgcgaccg ggactcagtg ccaagatcta gtggcggaac
    17101 ggtgtcgcca gcgctgcaac gagaccgctg tacaccttgc gcgccaccgc gcgccgatcg
    17161 tgctgctggg ccgtccgcgt ggtcttcgac cgacggaagg actcggcctc acgcacgcga
    17221 tcccggatct gctccctcgc catgtctccc gtgatcatcg tccgcctcct gggtcggtga
    17281 cgggactgac ggtatgcccc ggagcatgtc cgggctgtgt tcggaggcac ggaggacggg
    17341 tgactttggc cacagggagg atgtgtcgca cgtcacgcgg gcggcgatga tggccggcgt
    17401 gacgaccgtg gccgtgatcg ggctcggagc gatgggcgcg cgattcgcgc agcgcttcct
    17461 cgacgccggg cacgaggtga tcgtctggaa ccgcaccccg gagaagggtg aggacctcgt
    17521 ctcgcgcggt gcgacgccgg cggcgagccc ggccgaggcg gcccgcagcg ccgagggagt
    17581 tgtgacggtg gttgccgatc ccacggcgct gcgggcggtc acggaaggac cggatggcat
    17641 cgccgccggc gccggcggtg ggaccacggt gatcgagatg tcgaccgtgg ggtccgacgc
    17701 gatccgttgg cttcggtcgg cactcccgcc gggcgtgggc ctgctggacg ctccggtcct
    17761 cgggagcctg tcagaagccg agtcgggcac gctcctcgtg ttcgtcggcg gcccgccccg
    17821 tctcgcagaa cggtggaccc cgctcttcgg gacgctcggc tcggtgatcg tggccggtcc
    17881 cctcgggtcc ggagctgcgg cgaagctggt ggcgaacgcc accctggtcg gcaccctgac
    17941 gctcttgggc gaggcgatcg ccctcgcgga ccggctcggc ctggggcgga ggctcacgat
    18001 ggacatcctg gcgtcgacgc ccctcggccc ggcctcgaag aggcgccgcg aacagctcga
    18061 gtccggggac ttcccgctgc gattccgcct cgccctggct cggaaggacg ccgagctgat
    18121 ccaggaggcg gcggaggcgg cccacgcgga cacgcgcgtg ctcggggctg ccgcgacgtg
    18181 gttcgccgat gccgaggaag caggcctcgg cgatcgcgac tactcggaga tcctgcgtct
    18241 gatcacgcgg gagatgtcgg cctccggacc ggactgaccg tgttcgagcg attcacggac
    18301 cgagcgcggc gcgtcgtggt cctcgcccag acggaggcga gcatgctcaa ccacaactac
    18361 atcggcactg agcacatcct gctcgggctg atccacgaag gcgagggcgt cgccgcgcag
    18421 gtcttcgagt cgctcgacat ctcggtggag gccgcccgcc gacaggtcga agagatcatc
    18481 ggtcgaggcg gaggcgagat caccggccac atcccgttca cgccacgcgc caagaaggtc
    18541 ctcgagctgt cgctccgcga ggcgctccag ctgggacaca actacatcgg cacggagcat
    18601 ctcctgctcg ggctgatccg cgagggcgag ggcgtcgccg cgcaggtgct ccggacgctc
    18661 ggcgcggacc tcaaccgtgt gcgccaggcc gtgatccagg tcctctccgg accgacggcg
    18721 agcgcgaccg agcaggaggc tccgccgccg tggacgccgg catcgggccc aaactgcccg
    18781 tggtgcaacc tgcctttgga acacaccctc gcgcaccgca ccctcctcgt ccccgctgcc
    18841 gatgacgagg gtggtgagcc gatctccctc gacaccttgt tctgcacggg ctgtggtggc
    18901 ttggtgcgca cgtcgctgga ctaggacgcg ccgttgctag acttcggccg gccgcggtcc
    18961 catcgtctag aggcctagga catcaccctc tcaaggtgaa gacacgggtt caaatcccgt
    19021 tgggactacc agtcatagct cagctctggg tctagatcct gtctccgagg catcagggcg
    19081 ggacggatcg acggccccga gtcgcgcgtg tgagccggag gccgccgact cgtgactcgg
    19141 gagacccacg atcgcatcag atcgggtaat ggctcggccc tctcgctccc cgtatcagac
    19201 gagatgtcga aggagacgat cctgagcgag cctcgccagc gttcggtgga ggaggccttc
    19261 gtcgcgatgg cgcctcgcct ctggcgggcg ctgctgctct acgccgggga tcgcgacgtg
    19321 gcggacgacg ccgtctcgga ggcgttcacg caggccatga gcgcctccac gtcgatccgg
    19381 gacgtccgtg gatgggtctg gaccgccgcc ttccgcatcg ccgccggaga gatgcggcgc
    19441 cgggccgagc agagcgatga tcttgcgatc gaccttccgg tgcccgaggg catcgacccg
    19501 agctcgttga tcgacctcgt gcgggcgctc gaggaactca cagacatgca gcgacgggcc
    19561 gtgatcctca ggcattacat ggggtactcc aacgtcgaga tcgcccgcat cctcggctcc
    19621 agtccgtcgt cggtcggtgt ccggctgttc cggtcgaccc gcaggctgcg ccagctgctc
    19681 aacgaggagg gatgagatga ccacgtcgtt gaaggaccgt ttccgtcagg tcgatgcgat
    19741 ccccaccccg tgggacgagc gagcggccgc cgtgccgacg cagccgccgc aggccgcgtc
    19801 tcactgggct ccacgtgcac tcgtcctcgg tgcggctgcg gtcgccgtcg cgttcgtggc
    19861 catcgtcgcg ggtccggcgg tcgagcaacg acccgctcca cgcggtgacg cttcctggtt
    19921 ggtcggcgcc cagcaaagct gcgtcgagca gtactcggtc gaagcgcttg cgaaccgcga
    19981 gtacgcgttc gagggagtca tcggcgatgt tgcgggcccg gcggatcccg agagccccga
    20041 tcccgctcac ctcaccacga cggtgacgtt cgaggtcgag cgctggttct ggggcggttc
    20101 tgacgctgag atcgccctcc agacgtacgc cgcgacctcg tccgtcggtg agctcgagga
    20161 ttcggtcggc gcacgactcc tcgtctccgg cgacgacatc tacatctggg cctgcggctt
    20221 cacccagcca gcctcggagc aagcccggtc ggactacggg gctgcggcgg cagagaggag
    20281 tgcctgatgc ggcggctgat cgcgacgatc ctcacgatct cgttcgcgtt gctcaccggg
    20341 catccggcaa cggcggtacc gccccgggtg tcggtcagcg cggagcttcg gtgcctcgag
    20401 ggcggcggca tcgggatcga cctcaccatc gagaacaccg ggcgtcggat cgctcgcatc
    20461 gatcccgact tccacctgct ggtccagacg atcaggaagg gccgtcagcc gggagtcgtc
    20521 ctcttcgtgt tccctgctcc ggggttcgac cggatccctc cgggagagag ccgcaccttc
    20581 ttgctcacag cgggcgaacc gttcgaaggt gaacctgcga tcgacttctc cggcccgagg
    20641 atcatcctcg agaccgaggt gtggctgcgt ggtcgcgaca acccggtcgt ccgcaccctc
    20701 acctaccccg gatgtgctcc catcggtcgc cagtgaggga ggtcgaacca tcagggtgtc
    20761 ggttggagcc ctcctccaca tcccgcgcga aggttcttcg cgggagcgct ccccggcgac
    20821 gatatcctgc gggcatgggt cgggcccttc gcgatcctcg gatccgcctc ctcgtggctg
    20881 cgctggtcgt gaccctcgcg ctggcgctgt cgtggcatgt cgttgcgatg ggcctgcacg
    20941 ggatggcgat gatgctggga ctctgcctcg tggtcctcgc cggagtcgcc cttctcgctc
    21001 ccgcgagact cgaggctcgg gccgcgtcga tcccatcgtc gtcaatcgaa cggccgggag
    21061 gcgagcgggt cgaaccgttg ggccgccatc cccccgagga ggggatccga ctgagacatc
    21121 gatcacttcc gaacggaaga gctaccaacc gaccggaagg agatcacatg cacaccaaac
    21181 ctctcgtggt cgcggccgtt gccgcgttcg ccctgctgac cgtcgcctgt ggcgatggat
    21241 cggacgtcgg cagcggcacg gaacaagacc ctcgaacgat cgaggtcacg gcactggacg
    21301 accctgcatt cgacccgtcg acgatcgagg tggcggcggg cgagaccgtt cgcttcgtcg
    21361 tcaccaacac cggtgaggcg gatcacgagt tcgtcgtcgg tgacatggag atgcaggaga
    21421 tggccgagga gcaggccatg gaaggcatgc acggccatgc gcaggcgatg gctgcgctcg
    21481 cgctcgaacc cgacgcgacc gccgagacgg tgctcacgtt cgaggaggca ggcgagctct
    21541 tctacggctg ccacgtcgac gggcactacg aagcggggat ggtcggcacg atcaccgtca
    21601 cgtgatccct gggtggaccc ccgcggctcg accgcggggt tccgtctctg tatcggaatc
    21661 tccgcgcggg aggcgaagcc gcttcagcca cagcgcgttg accgccacga tgaggctcga
    21721 gcccgacatc gagagcgccg cgatctccgg ccgcagagtg agcccgaagg ccggctcgaa
    21781 cactccggcc gcgatcggca acgcgatcgc gttgtagccg atcgcccacc ccaggttctg
    21841 ccgcatcttg cggagcgtcc cgcgcccgat gcgcagggcg gtcggaacgt ccaggggatc
    21901 ggaccgcatc agcacgacgt cggcggtctc gatcgcgacg tcggtgcccg caccaatcgc
    21961 gatgccgaga tcggcctggg caagagccgg cgcgtcgttc accccgtcac ccaccatcgc
    22021 caccttcctt ccctcccgct gcagctcggc gatcttgccg gccttgtcgc cggggaggac
    22081 ctccgcgatg acggtgctca cgcccagctc ccccgcgatc cggcgggcgg tcgcctcgtt
    22141 gtcccccgtg agcatcacca ctccgacccc cgactccgcg agggccccca cggcggcgcg
    22201 cgaggtgggg cgagccgcat cggcgatccc gatcagcccg gccgctcggc catccacagc
    22261 ggcgatcacc gcggtcttcc cgctggccgc gagctccttt cgtcgctcct cgagggagcc
    22321 catgtcgatg ccctctcgct ccatgagccg gcggttgccc acggccacct gatgaccgtc
    22381 cacattggcg gtcgccccgt ggccggggat gttctcgaag gcttctgctc gcgcggtcgg
    22441 cacgtcgttt gcgtcggcgt ggtcgacgac ggcttcggca aggggatgct ccgactcgcg
    22501 ttcgaccgcg gcgaccagcc gcaggagctc gcgctcggac aaggcgtcgg cgatcacgtc
    22561 gctcacctcc ggctcgcccc tcgtgagcgt gccggtcttg tccatcacga cgaccccgat
    22621 gctcgccgcc tcctccaggg cgatcgcgta cttgaagagg atcccccgag tggcgccgag
    22681 cccggtgccc accatgatcg ccgtgggcgt cgccaagccc aaggcgtcgg gacaggtgat
    22741 cacgaccacg gtgatcgcga acagcatcgc ctcatcgacc gaacgtcccg cggccagcca
    22801 gaccaagaag gtgccggcgc cgccgatcaa ggccacgaac acgagccaga aggccgcccg
    22861 gtcggcgagc ttctgacccg gggccttcga gttctgggct tcctggacga gcttcacgat
    22921 ctgtgcgagc gcggtgtcgg ccccgatctt cgtcgcgcga acccgaaggg tcccgttctc
    22981 gttgatcgtg gcgccgatca cctccgaccc gggctccttg tggaccggga ggctctctcc
    23041 ggtcacgacc gactcgtcga cctcgctgtc accgtcttcg accacgccgt cgaccgcgat
    23101 cttcgctccc ggccggacca gcagcagatc gcccacggcg acctccgccg tcggaacctc
    23161 gacgggctcc ccgtcgcgga tcaagagcgc catcggcggg gcgagatcga ggagcgtccg
    23221 gatcgcgtcg ttcgcgccgc cgcgcgcccg catctcgaac cagtgcccca gcaggacgaa
    23281 ggtcgcgagc acggtcgccg cctcgtagaa gacctctccc ccacccgtca gggtgacacc
    23341 gaccgaatag agccacccca cgccgatcgc gaccgcgacc agcaccatca tgtcgagcgt
    23401 ccgcgcccgg agcgcccgca cggcgccgtc gaagaagatc cagccggagt agaagatcac
    23461 ggggaggctg aggatcagcg agaacacgtc gtctcgaagg ccaagaggcg cagtgacgtc
    23521 gaatcccagc acgtcgcgcc cgatcggcga ccagagcagg atcgggaccg agaggaacgc
    23581 ggccacgagg aaccggttcc gcatgtcgcg gaccatcgcg tccatcgaca tgcccgcgtg
    23641 cccgccgtgg cccatcgctt cctgaggcga tgggagcccc tccccggcgg ccatctcgtg
    23701 ggcctcgtgc tccactggaa tcgcgtggcc gtcatgccgc gcctctgtcg tggccacggt
    23761 cgcgccctgg tgcatcgtgt gggcgtgtga gaggtcgggc tccgccaacg ggtcgcacac
    23821 gtgcgtcggc acggactgac cggcgcagtg gagcccgcat tcctcgaccc atcgccggag
    23881 ctcagcgacc gaggtccgct tcgggtcgta cgtgaccgtc gccgtttgag ccaccgggtt
    23941 ggcctcgacg gcctccacgc ctcgccgccg accgaggacg gcttccacga tcgccttctc
    24001 ggaggcccag ttcagaccgc cggtgtgcag gacgacggta gtcaccttgt cgtcgtcgcc
    24061 ctgcagatcc tgccgccctg caccgtgact cgagtgatgt ggtctgtcgg ccttcgtgct
    24121 catcccctat cggagcccaa agttgcccgt cggctggtca ggcgacgaat cgctctggcg
    24181 tggccacgaa ctgctggtgg cagttctcgg agcagaagta gtaggtcgtg ccctcgtatt
    24241 cctcgctcgc cgcggccgtt gccgggtcga tgtccatccc gcacaccggg tccttcacgg
    24301 tcgccatcgc ttcctccttc gcgtcctctg gccgctcctg gtgctcgtgc acctcgacgc
    24361 tcactcggtc agccgtgtca cccttcgtgg gaagctccga ggctcggaag ctgcggagcc
    24421 ggttcgcgtt cgtcacaacc gagagcgagg acagcgccat cgccgcggcg gcgatgatcg
    24481 ggctcagcaa cacaccggtc gcagcgtaca gagcgccggc cgccaccggg atcccgagga
    24541 tgttgtagac gaaggcgaag aacaggttct gacggatgtt cctcatcgtc gaccggctga
    24601 gcccgacgga ggtgacgacg ccctccaggg caccggagat cagcgtcacg tcggcggcct
    24661 cgatcgcgac gtccgtcccg gtgccgatcg cgacccccac atccgcctgc gcgagcgccg
    24721 gagcgtcgtt gatcccgtca ccaaccatcg cgacgagctt cccctcgtcc tggagccgcc
    24781 ggacctccag cgccttgtct tcgggcagta cctcggcgag cacccgcgcg atgccgacct
    24841 cgcgcgcgac ggcctcggcc gtccgccggt tgtcgcccgt gatcatcacc acctcgagac
    24901 cgagctcccg aagcgcccgg atcgcgcgcg gcgagtcctc cttcagcgtg tcggcgacgg
    24961 cgaccacacc cgccggagcg ccgtcgacgg cggcgtagac cggcgtcttg ccctggccgg
    25021 cgagccgctc cccatcggct accaggctcg ccgcatcgat gccgcgatcc gccagcagct
    25081 gtctcgtccc gatcagcacc gttcgctcat cgaccgtggc ctcgatgccg cgcccggtca
    25141 ccgactggaa gtcggtgggc tcgacaagcg cgatcccccg ctcctcggcg ccccggacga
    25201 tcgcctggcc caaggggtgc tcagaggtgc gttcggcgga ggccaccagc cggacgaggt
    25261 cgtcctccga cgcggagtcg atggggacga tgtcggtgag cgacggctct cccttcgtga
    25321 tcgtcccggt cttgtccagg acgatcgtgt gcagcttgtg cgcggtctcc agcgcctcgg
    25381 ccgaccggat cagcaccccg tgctcggcac ccttgcccgt gcccaccatg atcgagagtg
    25441 gcgtggcgag cccgagcgcg cagggacacg cgatgatgag cacggagacg gccgatacga
    25501 gacccaacgt gagcgcgggg ctcggcccga gaacgaacca caccacgaac gtcccgatcg
    25561 cgatgaagat caccaccggc acgaagtagc tcgccaccag gtccgccagc cgctggatcg
    25621 gcgccttgga cgcctgcgcc tgctcgacca gctcgatgat ctgggcgagc atcgtgtcct
    25681 tgccgacctt cgtggcgcgg aagtggaacg cgcccgtctg gttgatggtg gccccgatga
    25741 cggcctcgtc cggacccttg gtgaccggga tcggctcccc cgtgaccatc gactcgtcga
    25801 tcgatgagcg tccgtccacg atctcgccgt ccaccgggat cttctcgccg ggacggaccg
    25861 cgaccacgtc gccgacctgc acatcgtcca ccggggtctc gtgctcctgc ccgtccctga
    25921 cgaccctcgc ggtctgtggc cgcaggccga tcagcttccg gatcgcttct ccggtgcccg
    25981 ccttcgcgcg ggcctcgaac agccggccga gcaggatcag cgtgaggatc acgccgaccg
    26041 cctcgaagta cacctcgcgg acctctgcgg ggaacagccc gggcgcgacc gtgacgagga
    26101 cgctgtaggc gaacgcggcc gtcgtcccga ccgtgatgag cgtattcatc tccgcggtgc
    26161 ggtgcagaag cgtccgccac ccaacggtgt ggatcggcca tcccgtgtag aggaagaccg
    26221 ggatggtcaa ggcgagctgg atccaccggt tcatcaggaa ctcgggcacc cacgccgcgt
    26281 cgaagatctc gctcgccatc acggagatca gcaccggcgc cgtcagcgca gcgccgacaa
    26341 ccacccgcca cgtgaggtcg cgaacctccg cccggcgtgc ggcggcctcg acgtcctccg
    26401 tcccgtccga cgccgctcgg ggtggctgct ggacccggta tccgatcgag gccacggcat
    26461 ggcgcatgtc ttcgaccgag accgccgacg gatcgaactc gaccgaggct cgctcgttga
    26521 cgacgtcggt ctgcacgctc cggacgccgg gcaggctctc cagttcggac tcgatggttc
    26581 ggaggcacgc agcgcaggcg acgccccctc cgcgctcgag cgcgagggcg acacgcccgg
    26641 ccgcctcgcc acccggctcc atcgagaccg tcctcgtctc ctga
//
LOCUS       DASUYD010000034         6686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1340960, whole genome shotgun sequence.
ACCESSION   DASUYD010000034 DASUYD010000000
VERSION     DASUYD010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340960"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..233)
                     /locus_tag="VF195_01805"
     tRNA            complement(158..233)
                     /locus_tag="VF195_01805"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Arg,seq:tct)
     gene            360..707
                     /locus_tag="VF195_01810"
     CDS             360..707
                     /locus_tag="VF195_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579594.1"
                     /translation="MADVGVGHRPYGHTMRDHRRLSLVRHPSVTIEAAAELVDVDVPT
                     VRGWAREGALIIEQRGDMEVVQLEEVKALAGRRRATKRGGLRDRLADSGMTPAGEVVN
                     IADLQGLARERAE"
     gene            715..1080
                     /locus_tag="VF195_01815"
     CDS             715..1080
                     /locus_tag="VF195_01815"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6579595.1"
                     /translation="MTDFGIRADGPHMFSVSGELDIATVPLMNVAIAHAVSQGGPITI
                     DMSFLTFMDSTGVGAILKSAKELATGCIVLHGVHDEVGRVIELMGVGEARQNLHVISC
                     FPPGEARSEMRSRRLDPSP"
     gene            1287..1895
                     /locus_tag="VF195_01820"
     CDS             1287..1895
                     /locus_tag="VF195_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579596.1"
                     /translation="MAVVDARVSDLAGGRLPPVCAKTGEPTDGQVKIRFSSAPGWTWI
                     LLFFGIFPFLIAQHYATVHVDSLLPLSDLAQRRVKVFNRLFIGLVALGAVVVIVGWVL
                     DTEAAVILVGLAMMIASAFVMFLALPFVLPSGAVIGEWVQLSFVHERFAAELDRFYRR
                     SVEPATGWNNSPTVKRWVLFVGAALILALLLALCFGGGETSA"
     gene            complement(2075..2145)
                     /locus_tag="VF195_01825"
     tRNA            complement(2075..2145)
                     /locus_tag="VF195_01825"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2111..2113),aa:Gly,seq:tcc)
     gene            2187..3059
                     /locus_tag="VF195_01830"
     CDS             2187..3059
                     /locus_tag="VF195_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX6579597.1"
                     /translation="MEVGFRLVDAFTETPFKGNRLCVVPEIPEGLSDDQMRMLALETN
                     FSETTFVTESHAGGYRMRIFTPDAELPFAGHPTIGTAFTLASEGRTSNRTVQTTALGD
                     VPVDVDLERGFAWMTQHRPVFGGEFDDRALVAEAAGLQADDLDPTLPMQVITTGLGPL
                     LVPIKDEATLRRAERNEPGCRRATEASGGECLYLFAIRGDGDVLARMFDASFGVGEDP
                     ATGSAAGPLGVYLASRGLAGMPGEAVVAQGEMVGRPSFLHLDVRPDDGSWRVRVGGGV
                     RIVGEGVFRVAIGD"
     gene            complement(3056..3454)
                     /locus_tag="VF195_01835"
     CDS             complement(3056..3454)
                     /locus_tag="VF195_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579598.1"
                     /translation="MRSRSGARSTISTVLSIALLIAIAGFAVLVGLQVRGDHPARFDV
                     TQAQGVECPVGEGSPACFSFGVTNLGSQPSLVECIATAPTGLASFLNDMPAYTSSAPF
                     EAGVTEQLIVKVDQGDQGAVSEPALVCRAL"
     gene            3493..3566
                     /locus_tag="VF195_01840"
     tRNA            3493..3566
                     /locus_tag="VF195_01840"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3527..3529,aa:Pro,seq:tgg)
     gene            complement(3575..4705)
                     /locus_tag="VF195_01845"
     CDS             complement(3575..4705)
                     /locus_tag="VF195_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003926035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6579599.1"
                     /translation="MTRYRDLGLSVGLLSPGPVNAITDVTGVRVGVTTLIEGHGPLEV
                     GTGPIRTGVTAIVPHEGIGEEPLFAGCHTLNGNGELTGLEWVRESGLLTTPIAITNTH
                     SVGVVRDALVAYEVRNRPKGPAFWSLPVVGETYDGALNDINGQHVRPEHLFAAIEQAS
                     GGPVPEGCVGGGTGMICHDFKGGTGTASRVVGGPGNSWTVGALVQANHGDRERLAVDG
                     VHVGEAIPTSEIPSAWDEEERLGSAAEGSIIVIVATDAPLVSHQLRRLAQRVSLGIAR
                     VGGGGGDSSGDIFLAFSTANAGRIMTSKTDAGPGPISVEMLPNREITSLFWGVIEATE
                     EAILNALVAAETLTGRDGITAHALPHDRLVETMTKHGRGPNR"
     gene            complement(4726..5034)
                     /locus_tag="VF195_01850"
     CDS             complement(4726..5034)
                     /locus_tag="VF195_01850"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEX6579600.1"
                     /translation="MSAAWWLVALVGVATIAIKGAGPVLLGGKPLPPRIGRIIGLLAP
                     ALLAALVAISTFGGDRALVLDERAVGVGAAAVAVAFKAPPLLVVVVAAVVTAVTRALA
                     "
     gene            complement(5031..5696)
                     /locus_tag="VF195_01855"
     CDS             complement(5031..5696)
                     /locus_tag="VF195_01855"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEX6579601.1"
                     /translation="MDEPGGSFSFAHGARKVAPFSIAVFGFGMSFGVLARPTMGTVAP
                     IVMSLTTFAGSAQFASASILGTGGTLAAAVIAAVLLNARYGPIGVAVAPSLAGPWWSR
                     FLRAQLVVDETWALSADGRGGHDPKLIVGAGLVLYAAWVSGTAAGVLFGDLLGDPARL
                     GLDAAFPALFLALLVPNLDRPQARVAAVLGATIALALTPVAPAGVPIVAAGLACLVGW
                     RRS"
     gene            5743..6207
                     /locus_tag="VF195_01860"
     CDS             5743..6207
                     /locus_tag="VF195_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579602.1"
                     /translation="MDLSSAVPDLSVGDIVTMLAGLPEPGDYSVHVKPLRYRDRPHLA
                     AWTDFEDRSITLQLPEPFFPFGEIVPYGAKRRTSGKGTRPRFIWLTEGITFRTQEEVL
                     RFSYCHEWMHWWLKEVRGTASAAETTCDRFALHNFRRRSVTEADALPSLRRR"
     gene            complement(6204..6494)
                     /locus_tag="VF195_01865"
     CDS             complement(6204..6494)
                     /locus_tag="VF195_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579603.1"
                     /translation="MSEADEVFAEQVARWRATSWRDLRKDLDDPVAYEIPGSDGVGYQ
                     FEVLVLWDDPRERTNLRVLFTGDDGRGWRMSGMHQASFVKATDDPFVGEDDA"
     gene            complement(6491..>6686)
                     /locus_tag="VF195_01870"
     CDS             complement(6491..>6686)
                     /locus_tag="VF195_01870"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020002033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX6579604.1"
                     /translation="PKVNRFRSLGDVPPETAESKAMSKDLKRRGFRFVGPTVCYAFMQ
                     ACGLVNDHVVSCFRYRQVAG"
BASE COUNT         1087 a         2235 c         2324 g         1040 t
ORIGIN      
        1 tgaagatcag gctcgagtgg cacgtggagc gggtgggaca caggtgccgg agccggtgtc
       61 gcccgggagc cggagccggg catgagggtg ggacacaggt gccggagccg atgtgggaca
      121 gaggtgccgg aacttcacag gccggcgcgc caacggctgg cgcgccgggc aggattcgaa
      181 cctgcgacct gaggattaga agtcccctgc tctatcccct gagctaccgg cgctcgctcg
      241 gagcgtacct tcatgttgag ggttccgaga gacgtggggg cctggagctg tccgggtcat
      301 cggtcctagg tacaacggct cggttgaccc gagccatccg ccagggaacc gtagcccgag
      361 tggccgatgt gggcgtcggc catcgaccct acggtcacac catgagagac caccgacgcc
      421 tttcccttgt ccgccacccc tccgtgacga tcgaagccgc cgccgagcta gtcgacgttg
      481 acgttcccac tgtccgcggg tgggcacgcg agggagctct gatcatcgag cagcgaggcg
      541 acatggaagt cgtccagctg gaagaggtga aggcgctcgc agggaggcgg cgagcgacga
      601 aacgcggagg actccgtgac cgactggctg acagcggcat gacgccggcg ggggaggtcg
      661 tgaacatcgc tgaccttcaa gggttggcga gagagcgagc ggagtagcgc aacgatgacg
      721 gacttcggga tcagggccga cggtccgcac atgttctcgg tgagcgggga gctggacatc
      781 gcgaccgtgc ctctcatgaa cgtggcgatc gcacatgccg tgtcgcaggg tggacccatc
      841 acgatcgaca tgagcttcct gaccttcatg gattccacag gggtgggggc gatcctcaag
      901 tcggcgaagg aattggctac cggatgcatc gttcttcacg gcgtccacga cgaggtcggc
      961 agggtgatcg aactcatggg tgttggcgaa gcaaggcaga acctccacgt catctcctgc
     1021 ttccccccag gggaggcgcg gtcggagatg cgatcgaggc gcttggatcc atcgccctga
     1081 ccgagtggag tgagtgacgg agatcggatc cacaccacca aggggttcgc cgaggcggcg
     1141 ctcgaccgga aacgagcccg acgcttgcga ggccgccccg ccacctcggc cgcgtccggc
     1201 caccctcgtc gttggcgttc ataaggctat cccggggggg tgtaacgtcg cccgcgatgg
     1261 acgtcggaga gtgcgaggtt gagtagttgg cggtggtaga cgcacgtgtg tccgatctcg
     1321 cagggggcag gctgccaccg gtgtgcgcga agacgggtga gccgaccgat ggacaagtca
     1381 agatccggtt ctcttcggcc ccggggtgga cctggatcct cctgttcttc ggcatcttcc
     1441 ccttcctgat cgcccagcac tacgccacgg ttcacgtaga cagcctctta ccgctgtcgg
     1501 acctcgcaca gcggcgcgtg aaggtcttca accggctctt catcgggctc gtcgccctcg
     1561 gcgcggtcgt cgtcatcgtt gggtgggtgc ttgataccga ggcggctgta atccttgtgg
     1621 gactggcgat gatgatcgcc tccgccttcg tgatgtttct ggcgttgccg ttcgtgctac
     1681 cgagcggtgc ggtcataggc gagtgggtgc agttgtcctt cgttcacgag cgcttcgctg
     1741 ctgagctcga ccgcttctac aggcgctccg tcgagccagc tacaggctgg aacaacagcc
     1801 ccaccgtcaa gaggtgggtg ctgttcgtcg gggccgcact gatcctcgct ctactgctgg
     1861 ccctctgctt cggtggaggc gaaacgtcag cttgacacga ggctgaggaa agggggtgac
     1921 gacagcaaga aatggacgac cagagctgag acctacgagc gggtcgccga ggagttcgta
     1981 tggccgggtc ggtcgttccg ctgagaggga ccggacgggg ttgagcagcc catcggaaag
     2041 tctctcgacc tgcgaggatg gacgaaacga gaagagcggg tgacgggaat cgaacccgca
     2101 ccaccagctt ggaaggctgg agctctacca ttgagctaca cccgcgctga gccagcctag
     2161 caacacccgg ctgagatact tggccggtgg aagtcggttt ccggctcgtt gacgcgttca
     2221 ccgagacacc cttcaagggc aaccggctct gcgtcgtgcc cgagatcccc gagggcctct
     2281 ccgacgatca gatgcggatg ctcgcgctcg agacgaactt ctccgagacg acgttcgtga
     2341 ccgaatcgca cgccggcggc tatcggatgc ggatcttcac gccggacgcc gagttgcctt
     2401 tcgccgggca tcccacgatc ggcactgcgt tcaccctggc gagcgagggc cggacgtcca
     2461 accgaacggt ccagacgacc gcgctcggcg acgtgccggt cgacgtcgac ctcgaacgcg
     2521 gcttcgcctg gatgacgcag catcggccgg tgttcggtgg ggagttcgac gatcgcgcgc
     2581 tcgtcgccga ggccgccggt ctgcaagccg acgacctcga tccgacgctc cctatgcagg
     2641 tgatcacgac cgggctcggc ccgttgctcg tgccgatcaa ggacgaggcc accctgcgcc
     2701 gggcggaacg caacgagccg ggatgccggc gcgcgaccga ggcgtcgggt ggcgagtgcc
     2761 tctacctgtt cgcgatccga ggggacggtg acgtgctcgc gcggatgttc gacgcatcct
     2821 tcggtgtcgg cgaggatccc gcgaccgggt cggccgccgg tccgctcggc gtctacctcg
     2881 catcgcgcgg cctcgcgggg atgcccggtg aggccgtggt cgcccagggc gagatggtgg
     2941 gacgcccgag cttcctgcac ctcgacgtcc ggcccgacga tggctcgtgg agggtgcggg
     3001 tgggcggcgg cgtccggatc gtcggtgagg gcgtcttccg agtggcgatc ggggactaga
     3061 gggccctgca gaccaacgcc ggttcggaca cggcgccctg gtccccctga tcgaccttga
     3121 cgatcagctg ctcggtgacc ccggcctcga acggggccga gctcgtgtag gccggcatgt
     3181 cgttcaggaa gctggccagg ccggtcgggg ccgttgcgat gcactccacg agggacggct
     3241 ggctgccgag gttcgtcact ccgaagctga agcatgccgg gctgccttcg ccgaccgggc
     3301 actccacacc ctgggcttgt gtgacgtcga agcgagccgg gtgatcaccg cgaacctgca
     3361 agccgacgag gaccgcgaac cccgcgatcg cgatgagcaa cgcgatcgag aggaccgtgc
     3421 tgatggtgct ccgggccccg gaccgcgaac gcatgcccca gagtaccggg acgtagaatc
     3481 gctgcacaag agcggggcgt agcgcagcct ggtagcgcac gtgctttggg agcacgaggt
     3541 cgcgggttcg aatcccgccg ccccgaccgc agggctagcg gttcggtccc cgtccgtgct
     3601 tcgtcatcgt ctccaccaat cggtcatgag ggagcgcgtg ggcggtgatg ccgtcgcggc
     3661 cggtcaacgt ctccgccgcg acgagcgcgt tcaggatcgc ctcttcggtc gcctcgatca
     3721 cgccccagaa cagcgaggtg atctcgcggt tcggaagcat ctcgacgctg atggggccgg
     3781 ggccggcatc ggtcttcgag gtcatgatcc gtccggcgtt cgcggttgag aacgcgagga
     3841 agatgtcgcc gctggaatcg cctccgccgc cccccaccct cgcgatcccg aggctcaccc
     3901 gctgggccag ccgccggagc tggtgagaga cgagcggcgc gtccgtggcc acgatcacga
     3961 tgatcgatcc ctcggcggcc gagccgagcc gctcctcctc gtcccaagcg gacgggatct
     4021 cgctcgtggg gatcgcttcg ccgacgtgca cgccgtcgac cgcgagccgc tcgcggtctc
     4081 cgtgattggc ctgcacgagc gctccgacgg tccagctgtt ccccggaccc ccgaccacgc
     4141 gcgatgcggt tccggtgcct cccttgaagt cgtggcagat catccccgtg ccgccgccga
     4201 cgcagccctc ggggaccggg ccgcccgagg cctgttcgat cgcggcgaac agatgctccg
     4261 gccggacgtg ctgcccattg atgtcgttca gcgcgccgtc gtaggtctcg ccgaccacgg
     4321 ggaggctcca gaacgccgga cccttcgggc ggttccgcac ctcgtaggcg acgagcgcgt
     4381 cgcggacgac accgacgctg tgagtgttgg tgatcgcgat cggcgtggtg aggagcccgg
     4441 actcgcgcac ccactcgagc ccggtcagct cgccgttgcc gttgagcgtg tggcagccgg
     4501 cgaagagcgg ctcctctccg atcccctcgt gcgggacgat cgccgtgaca cccgtccgga
     4561 tcggtcccgt gccgacctcg agcgggccgt gcccttcgat gagggtcgtg acgcccacgc
     4621 ggacgccggt cacgtccgtg atcgcgttca ccggtccggg ggagaggagc ccgaccgaca
     4681 ggccgaggtc gcggtagcgc gtcatcgggg tcgaacccta cagccctagg cgagcgccct
     4741 cgtgacggcg gtgaccaccg ccgcaacgac caccacgagg agcgggggag ccttgaaggc
     4801 cacagcgacc gcagcggctc cgacgccgac cgcgcgctcg tcgaggacga gcgcgcgatc
     4861 tcccccgaac gtgctgatgg cgacgagcgc agctagcagc gcgggcgcga ggaggccgat
     4921 gatccgtccg atcctcggcg ggaggggttt gccgccgagg agcaccggcc ccgcaccttt
     4981 gatcgcgatc gtcgccaccc cgacgagggc gaccagccac cacgcagcgc tcatgagcgc
     5041 ctccatccca cgaggcaggc caggccggcc gcgacgatcg gcacgccggc cggtgcgacc
     5101 ggggtcaggg cgagcgcgat cgtcgcgccg aggaccgccg ccacgcgggc ctgcggacga
     5161 tccaggttcg gcaccaggag cgcgaggaac aacgccggga acgccgcgtc gagtcccaac
     5221 cgggcggggt cgccgaggag gtcgccgaac agcacacccg ccgcggtccc gctgacccat
     5281 gccgcgtaga ggacgagccc ggcgccaacg atgagcttcg gatcgtgccc gccgcggccg
     5341 tcggcggaca acgcccaggt ctcgtccacg acgagctggg cgcggaggaa acgggaccac
     5401 cagggcccgg ccagcgaggg cgccacggcg acgccgatcg gtccgtaacg cgcgttcaac
     5461 aggaccgcgg cgatcacggc cgccgcgagc gtcccgccgg tgccgaggat cgaggcggag
     5521 gcgaactgcg ccgagcccgc gaacgtggtc aacgacatca cgatcggggc gaccgtcccc
     5581 atcgtcggac gggccagcac cccgaacgac atcccgaatc cgaacaccgc gatcgagaac
     5641 ggcgcgacct tccttgcacc gtgggcgaag ctgaaggagc cgccgggctc gtccatcggt
     5701 ccgcggaccc tatcagtggc ccccgcgtag gctgcagtgc cgatggacct gtcctccgcc
     5761 gtgccggatc tctccgtcgg ggacatcgtg acgatgctcg cgggtctgcc ggaaccgggg
     5821 gactactccg tccatgtgaa gccgctccgg taccgagacc gcccacacct cgcggcctgg
     5881 acggacttcg aggatcgctc aatcacgttg cagctgccgg agccgttctt cccgttcggc
     5941 gagatcgttc cctacggagc gaagcggagg accagtggga aggggactcg ccctcgtttc
     6001 atctggctca cggagggcat cacgttccgc acgcaagagg aagtgctgcg cttctcctac
     6061 tgccacgagt ggatgcactg gtggctcaag gaagtacgag ggacggcgtc ggcggccgag
     6121 accacgtgcg accgcttcgc cctccacaac ttccgccggc gatcggtgac ggaggccgac
     6181 gcgctccctt ccttgaggcg gcgttaggcg tcgtcctcgc cgacgaacgg gtcgtcggtt
     6241 gccttgacga agctcgcctg gtgcatgccg gacatgcgcc agcccctacc gtcgtcgccg
     6301 gtgaacagga cgcgaaggtt ggtgcgctct ctcggatcgt cccagagcac gagcacctcg
     6361 aactggtacc cgactccgtc ggagccaggg atctcgtacg cgaccggatc gtccagatcc
     6421 ttccggaggt cccgccagga ggtcgcacgc caccgcgcca cctgctccgc gaagacctcg
     6481 tccgcttcgc tcaccctgcc acctgccggt agcggaagca gctcacgacg tggtcgttca
     6541 cgaggccgca ggcctgcatg aacgcgtagc agacggtcgg tccgacgaac cggaacccgc
     6601 gccgcttcag gtccttcgac atggccttcg actccgcggt ctccgggggg acgtccccga
     6661 ggctgcggaa ccggttcacc ttcggc
//
LOCUS       DASUYD010000035        10102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1341056, whole genome shotgun sequence.
ACCESSION   DASUYD010000035 DASUYD010000000
VERSION     DASUYD010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1341056"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..831
                     /locus_tag="VF195_01875"
     CDS             <1..831
                     /locus_tag="VF195_01875"
                     /inference="COORDINATES: protein motif:HMM:NF020919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1996 domain-containing protein"
                     /protein_id="HEX6579605.1"
                     /translation="RARDGRRRRGSLLLVVITLTTGLVPLSAPSASASARIHIECDFH
                     HRKTDDPIVYPRRPGAAHLHLFFGNKSTDAFSTWTSLRRAGTNCNRTVDKAAYWMPAV
                     YKNGSLVKAVAGHFYYRGVHHNLSAIKAYPPGLKIIAGNHEATKPQSTRVVAWSCQGS
                     SSTGQATMRDCGAGGHVKVLIKFPSCWDGKRKDSPNHKAHMRYATRLGGGRRGCPRTH
                     PVPVPELTMAVSFAMRDGAGVDLSSGKFYTMHADFFNAWNQTALKKLIRNCIHANKEC
                     "
     gene            complement(1104..3071)
                     /locus_tag="VF195_01880"
     CDS             complement(1104..3071)
                     /locus_tag="VF195_01880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX6579606.1"
                     /translation="MTERATGPVVPVEAEHVRALDGMRAIAVLLVILFHLRVPGFHAG
                     FVGVDIFFVLSGFLITSLLLEEVRRTGRVSLPAFWARRARRLLPALVLVLLTVGVATA
                     MTATYTERASLRGDLLATTGYVANWHFIETSTYFADIGVDSPLEHTWSLAIEEQFYLL
                     WPLLVTAVAAVGVRLRVGVGTLALVGVIASAALLALLWAPGDVERAYMGTDARIFEPL
                     LGAAGAAFVASPGGRTRISRAGRWVLAAGAAALCIGLVTITPGGRGYFLGGAVLISAG
                     TLAIMAPLWVGAGGVTARSILSWGPLVWIGVVSYGAYLWHWPITLWLRVRDPGVDGLV
                     LRQVTAALLTFGIAAISFALVERPIRSGTFLAARRTRSGSRGRHRSGALRSRTAVTLA
                     AVPVTLVGVASVSLGLTRVPPIDAGDPVVMMVGDSVPARMGAPLERAFAEEGWRFVTA
                     ALGGCPPTGETPVRPDGTPWPGSVPGCRAEVAKRQDALLGTVDPDVVIWWDRFSVSGF
                     RDLHGVVVPAGSDRFWSLRRDALDAAVRRLGARGAIVVLVGAEPPAESVLDRCAVVGC
                     DWPLFQIAHYEDVTVPWNAMLRDYTEGHPERSTFISITDVVCREDVAPCDDRIGGITA
                     RRDGVHYEGAGEEAVIDSLLVLLGPSMERFE"
     gene            complement(3137..3568)
                     /locus_tag="VF195_01885"
     CDS             complement(3137..3568)
                     /locus_tag="VF195_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX6579607.1"
                     /translation="MTVIADGLPSPHLQRKIHEVQLFLAQNLSHDVDLNAIAAHASLS
                     PYYFTRQFTAMVGMPPYRYLISLRIARAAHLLRESDLTVTQILHRVGFHSPSHFTTTF
                     RRHMGMSPTDYRRRADFEQNANRFSRAIDPLVHQPEHRLSR"
     gene            complement(3657..5048)
                     /gene="fumC"
                     /locus_tag="VF195_01890"
     CDS             complement(3657..5048)
                     /gene="fumC"
                     /locus_tag="VF195_01890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX6579608.1"
                     /translation="MTDSRTETDSIGSIEVPADRYWGAQTQRSLHHFSIGDPAADRMP
                     MEVVRALALLKKAAAMENADRGLLDRSLADLIVAAADEIFAGTLDDHFPLYVWQTGSG
                     TQTNMNVNEVISNRAIEIAGGELGSKTPVHPNDHVNLSQSSNDTFPTAMHIATAMQLA
                     ERLLPSVRALRDALDAKSTAWADIVKIGRTHLQDAVPLTLGQEFSGYVAQLDDDHERI
                     EQTLPRLYELAIGATAVGTGLNAPDGFGDACAARLADFTGLPFVRAPNLFAALAANDS
                     IVHASGALATLAASLTKVANDVRLLGSGPRAGIGELVLPENEPGSSIMPGKVNPTQSE
                     AVTMVATQVFGNDTTIKIAGSQGTFELNVFKPVMIRNLLHSARLLADVCRTFREFCIE
                     GLEPNRVRIEELVHRSLMLVTALTPKLGYDKAAEIAKKAHHEGSTLREASLALGYLSE
                     AEYDEIVVPEQMI"
     gene            complement(5132..7438)
                     /locus_tag="VF195_01895"
     CDS             complement(5132..7438)
                     /locus_tag="VF195_01895"
                     /inference="COORDINATES: protein motif:HMM:NF044618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M6"
                     /protein_id="HEX6579609.1"
                     /translation="MRRTLIAALVVALTAVSVPGANTGLAATVTSARQAPERGSASDI
                     VQTPPDLRAKIRKQGAAAVSRTARAMTLALAEKGEIRTWLGLDDVAGAFYTKNYRLRG
                     VGEHIEVWTAAGSRKFNGVRSTGLRFQRDDCRNGERTMISSAQIRYFIDQFDNNIYPT
                     ESELFSVPPDRDGSEAPATEALGLPADYYTGDGDDIVVLIDNVRDDNFYDVNNTQEFS
                     YIAGFFSSGLNGFFDRNVMTIDGFDWLHRTGLTPPNDPVPGDPCASAPARPALYEGVF
                     AHEYQHLLLSYVDPPEATWVNEGLSDTAIALTGYGDPATPITDLGFDSHIQCLLGNNA
                     VQTPANPNPRPGGPENSLNVWGDQNFDHEQEILCDYGAAYSFLLWLADSYGDEVLTTL
                     HNDSENQGFEAIQAVLDVVDPGLTMSQAIDIWQATLALDAVLDDGATLVGGDFETYSV
                     ERLHAAINWETGAPENDAYDTPGAPPNGGDFVRLMDAGGAFLSAGDVTDVEFQGAQEL
                     PALPVTWEVDTTPPSGADGAALHSPVGDNLNEVIVRDVTVPAGSPQLTFDAAWDLETT
                     FDFGYAQITTDGGDTYTSLECTDTVDDTDPALGNVGPGFGHGFNGENPTPVFAPQTCD
                     LTPYAGMTVGLAFRVFNDGGVHMEGFWVDNVAIDGTVITDGSTLDGWLSATEYNPIEV
                     EGYTVQLVAYDSAGGGEAHLFSVPVDDTFHGVLMDAAMAEAIGTTADVVAAIVTYHDG
                     TQLVTQYAPYTLTVNGVTQPGGSAPVLP"
     gene            7633..8187
                     /locus_tag="VF195_01900"
     CDS             7633..8187
                     /locus_tag="VF195_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014214510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579610.1"
                     /translation="METWVWVVVIAAVAVVAVVAWLLSRQARSRGLQREFGSEYERTM
                     ARAGDRGVAEADLLERRDRVSALDVRPLSQADHDRFASEWTKVQAEFVDAPSEAVADA
                     DELIQQVMATRGYPVEDFDRRAADVSVEHPDVVENYRSAHSIAVKEARDTDGDTEALR
                     KAMVYYRSLFDELLVVDDAGDRRN"
     gene            complement(8184..8813)
                     /locus_tag="VF195_01905"
     CDS             complement(8184..8813)
                     /locus_tag="VF195_01905"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX6579611.1"
                     /translation="MRFALVTELVEWVRPFFGTFGYAIVSVAMFFESAALTGIVVPGD
                     VILAIGGVYAAEGELGLGAVIAFGAFFGLLGETTGYLVGRRYGDSLLRRLPILRRFEA
                     RLDQVEDSIESNAGKTIVVGRFVTGAAGLIPFIAGASRVPARKFFAFTIPTMLLWSTA
                     VALLGFLVGNHVETIDRILKRVGWVGLGLVVLTVGIWIWRHRRAPEPGS"
     gene            8940..9854
                     /locus_tag="VF195_01910"
     CDS             8940..9854
                     /locus_tag="VF195_01910"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6579612.1"
                     /translation="MSTVATVPETHHQDAGDARDQLRRSGTLPLLRDAAIRLRAAHGT
                     TYARALGHSSLLTLIPAVIAVIGFVAVFDLTGFARVLQDAATSFAPGPAGSILTRALG
                     TARSSAGPSALVVGLVGMLFSGTIGMTHLERAADRIYGVEEDRSPRERLGLAIMLAAT
                     VEVMLMIGLAVIVAGGSIGEGSDGGAGSGLWSFVRWPVGVGLVAIAMTIIFRVVPNRH
                     QPQFSWLLSGTTVSVTAWVAATILLGLVYEHASLLGDAFGPLIGVVALLVWAYATGLA
                     VLFGLAFAAQLEAERAGEHLKARDEPSR"
     gene            9869..9942
                     /locus_tag="VF195_01915"
     tRNA            9869..9942
                     /locus_tag="VF195_01915"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9903..9905,aa:Ile,seq:cat)
BASE COUNT         1619 a         3388 c         3532 g         1563 t
ORIGIN      
        1 cgcgcacgcg acggcagacg gcggcgcgga tcgctgctcc tggtcgtcat cacgctcacc
       61 accggcctgg tgccgctctc ggcgccgtct gcgtcggcga gtgcccggat ccacatcgag
      121 tgcgacttcc accatcgcaa gacggacgac ccgatcgtct atccgagacg gcccggggcc
      181 gcgcacctgc atctcttctt cggcaacaag tcgacggacg ccttctcgac ctggacctct
      241 ctgcgtcggg cgggtacgaa ctgcaaccga acggtcgaca aggcggcgta ctggatgccg
      301 gccgtgtaca agaacgggtc cctcgtcaag gcggtggccg gccatttcta ctaccggggt
      361 gtccaccaca acctgagtgc gatcaaggcg tatccgccgg ggctgaagat catcgctggg
      421 aaccatgagg cgaccaaacc gcagagcacg agggtggtcg cgtggtcgtg ccaggggagc
      481 agcagcacgg ggcaagcgac gatgcgcgac tgcggagccg gcgggcacgt caaggtcttg
      541 atcaagttcc cctcgtgctg ggacggcaag cggaaggaca gcccgaacca caaggcgcac
      601 atgcgctacg cgacgcggct cggcggtggg aggcgaggct gtccgaggac ccatcccgtt
      661 cccgtcccgg agctcacgat ggcggtctcc ttcgcgatgc gcgacggcgc cggcgtggac
      721 ctgtcgagcg ggaagttcta cacgatgcac gccgacttct tcaacgcgtg gaaccagacc
      781 gccctgaaga agctgatccg gaactgcatc cacgcgaaca aggagtgctg acggtccgca
      841 cccctagctg atcgggtccg cccgagacgt ggagccccct ccgaccgccg ccagcaggcc
      901 gatggggcat cgggagccca tctcgacatc cgggagaccg cgtaaccctt cgccgctccc
      961 cggatgtgtg aatcacgaca ccatcgggta cgaggctctc gtgaacctga caggaggagg
     1021 tgaccaaaga tgatctggac catcctggct gtgatcggat tgatcgccgt cctcatgtgg
     1081 ctgttctaga cggcgagcgg tagctactcg aaccgctcca tggacgggcc gagcagcacg
     1141 aggagcgaat cgatcaccgc ttcctcgccg gccccttcat agtggacccc gtcgcgacgc
     1201 gccgtgatcc ccccgatacg gtcgtcgcac ggggccacat cttctctgca cacgacgtct
     1261 gtgatgctga tgaacgtgct ccgctcggga tgtccctccg tgtaatcacg gagcatcgcg
     1321 ttccacggga cggtgacgtc ctcgtagtga gcgatctgga agagcggcca atcgcagccg
     1381 acgacggcgc agcgatcgag cacgctctcg gcgggaggct ccgcgccgac gagcacgacg
     1441 atcgctcccc gtgccccgag ccgacggacc gccgcgtcca gcgcatcgcg ccgcagcgac
     1501 cagaaccggt cggagcccgc cggcacgacg acgccgtgga ggtcgcggaa ccccgagacc
     1561 gagaaacggt cccaccagat caccacgtcg ggatcgaccg ttccgagcag cgcatcctgg
     1621 cgcttcgcga cctcggcccg acagcccggg accgagccgg gccacggtgt gccgtccggt
     1681 cggacgggag tctcgccggt cggcgggcat ccgcccagcg ccgccgtgac gaagcgccat
     1741 ccctcctccg cgaacgcgcg ctcgagcggc gcgcccatcc gggccgggac ggagtctccg
     1801 accatcatca ccacggggtc gccggcgtcg atcggcggca ccctcgtcag cccgagggac
     1861 acgctcgcca cgccgacgag cgtgaccggg accgcggcga gcgtgaccgc ggttcggctg
     1921 cggagcgccc cgctgcggtg ccgacctcgt gagcccgatc gcgtgcgccg ggcggcgagg
     1981 aaggtgccgc tccggatcgg cctctccacg agagcgaagg agatcgccgc gatcccgaag
     2041 gtgaggagcg cggcggtgac ctgccgcagc accagcccgt ccacccctgg gtcccggacc
     2101 cgcagccaga gtgtgatggg ccagtgccag aggtaggcgc cgtaggagac gacgccgatc
     2161 cacaccagcg gcccccacga aaggatcgac cgggcggtga cgcctccggc gcccacccac
     2221 aggggggcca tgatcgcgag cgtgccggcg gagatcagga cggcgccgcc gaggaagtag
     2281 ccgcgaccgc ccggcgtgat ggtcacgagc ccgatgcaca gggccgccgc accggccgcc
     2341 agcacccatc gcccggctcg ggagatgcgg gtgcgaccac ccggtgaggc gacgaacgcc
     2401 gcgccagccg cgccgagcaa cggctcgaag atcctcgcgt cggtccccat gtacgcacgc
     2461 tcgacatccc ccggcgccca cagcagggcg agcagggcgg cggacgcgat caccccgacg
     2521 agcgcgagcg tgcccacgcc cacacggagg cgcacgccca cggccgccac ggcggttacg
     2581 agcagcggcc acaggaggta gaactgctcc tcgatcgcga gcgaccaggt gtgctcgagc
     2641 ggtgagtcga cgccgatatc cgcgaagtac gtcgaggttt cgatgaagtg ccagttcgcc
     2701 acgtacccgg tcgtcgccag gagatcgccg cgcaaggagg cgcgctcggt gtaggtggct
     2761 gtcatcgccg ttgccacgcc gaccgtgagc agcacgagga ccagggccgg tagcagccgc
     2821 cgcgctcgac gagcccagaa cgcgggaagg gagacacggc ccgttcgcct gacctcctcg
     2881 agcagcagcg acgtgatcag gaatcccgac agcacgaaga agatgtcgac gccgacgaag
     2941 cctgcgtgga acccggggac gcggaggtgg aacaggatca cgaggaggac cgcgatcgcg
     3001 cgcataccgt cgagtgcgcg gacgtgctcg gcctccaccg gaaccaccgg tccggtcgct
     3061 cgctcggtca cctgccctcc gcgcaagagc atagcggggt gtgccctggg aacggatcgc
     3121 cggcgagcga ctacaactag cggctcagcc ggtgctccgg ctggtggacc agtggatcga
     3181 tcgcacggct gaaccggttc gcgttctgct cgaagtcggc tcggcggcgg tagtcggtgg
     3241 gactcatccc catgtgtcgc cggaaagtgg tcgtgaaatg gctggggctg tggaacccga
     3301 cccgatgcag gatctgcgtg accgtgaggt ccgactcacg cagcaggtgc gccgcgcgcg
     3361 cgatgcggag ggagatcagg taccggtacg gcggcatccc caccatcgcc gtgaactgcc
     3421 gggtgaagta gtacgggctc agggacgcgt gcgcagcgat cgcgttcaga tcgacgtcgt
     3481 gcgagaggtt ctgcgctagg aagagttgca cctcgtggat cttgcgctgg agatgcgggc
     3541 tcgggagccc gtcagcgatc accgtcatgc ggcccccatc gtcttccgta caatcgtacg
     3601 ccgttcgagg aacggctggc tttcggaatc ctgcccgatt cggacgtcgt ccacgctcag
     3661 atcatctgct ccggaaccac gatctcgtcg tattcggcct ccgagaggta gccgagcgcg
     3721 agcgaagctt ccctcagcgt gctgccctcg tggtgggcct tcttcgcgat ctccgcggcc
     3781 ttgtcgtagc ccagcttcgg cgtgagcgcc gtcaccagca tcagggatcg atgcacgagt
     3841 tcctcgatcc gcacccggtt cggctccaga ccctcgatgc agaactcgcg gaaggtccgg
     3901 cagacatcgg cgaggagtcg ggcggaatgc agcaggttcc ggatcatcac cggcttgaag
     3961 acgttcagtt cgaaggtgcc ctgcgagccc gcgatcttga tcgtcgtgtc gttcccgaac
     4021 acctgcgtcg ccaccatcgt cacggcttcg ctctgggtcg ggttcacctt gcccggcatg
     4081 atcgaggagc cgggctcgtt ctcggggagg acgagctcgc cgatcccggc gcgcggcccg
     4141 gagccgagca ggcggacgtc gttcgcgacc ttcgtcagcg atgccgcgag ggtcgcgagc
     4201 gcgccgctcg cgtggacgat cgagtcgttg gcggcgagcg ccgcgaagag gttgggggcg
     4261 cggacgaagg gcaggccggt gaagtcggcg agcctggccg cgcacgcgtc gccgaatccg
     4321 tccggcgcgt tgaggccggt gccgactgcg gtcgcgccta tcgcgagctc gtagaggcgg
     4381 gggagcgtct gctcgatccg ctcgtggtcg tcgtcgagct gggcgacgta gccggagaac
     4441 tcttgcccga gggtgagcgg gacggcgtcc tgcagatggg tccgaccgat cttcacgatg
     4501 tcggcccagg cggtcgactt cgcatcgagc gcatcgcgca gcgcacggac ggacggcagg
     4561 agccgttcgg cgagctgcat cgcggtggcg atatgcatcg cggtcgggaa cgtgtcgttc
     4621 gaggactgcg agaggttgac gtggtcgttc ggatggacgg gggtcttcga gccgagctca
     4681 cctcccgcga tctcgatcgc ccggttcgag atgacctcgt tcacgttcat gttggtctgg
     4741 gtgcccgacc ccgtctgcca cacgtagagg gggaagtggt cgtcgagggt gccggcgaag
     4801 atctcgtcgg cggccgcgac gatcaggtcc gcgagcgacc ggtccaggag accgcgatcc
     4861 gcgttctcca tcgccgcggc cttcttgagg agggcgaggg cgcgcaccac ctccatgggc
     4921 atgcggtccg ctgcgggatc gccgatcgag aagtgatgca gcgaccgctg ggtctgcgcg
     4981 ccccaatagc ggtcggccgg gacctcgatg gatccgatcg agtcggtctc ggttcgggag
     5041 tcggtcatcg tgaccccttc atcctcccac gccacgagac cggagatgcg tcggggggcg
     5101 gcccgttccg ggccgccccc cgacaccgcg ttcagggaag gacgggtgct gatcctcccg
     5161 gctgcgtgac gccgttcacg gtgagcgtgt acggcgcgta ctgcgtgacc agctgcgtgc
     5221 cgtcgtggta ggtgacgatc gccgccacca cgtcggccgt ggtcccgatc gcctcggcca
     5281 tcgcggcgtc catcagcacc ccatggaagg tgtcgtccac ggggacgctg aacaggtgcg
     5341 cttcaccacc gcccgccgag tcgtaggcca cgagctgcac cgtgtagccc tcgacctcga
     5401 tcgggttgta ctcggtggcg gacagccagc catcgagggt cgagccgtcg gtgatcacgg
     5461 tgccgtcgat cgcgacgttg tccacccaga acccttccat gtggacgccc ccgtcgttga
     5521 agacgcggaa ggcgagcccc acggtcatgc ccgcgtaggg cgtcaggtcg caggtctgcg
     5581 gcgcgaacac cggtgtgggg ttctcaccgt tgaacccgtg accgaagccc ggacccacgt
     5641 tgcccagcgc cggatccgtg tcgtccacgg tgtccgtgca ctcgaggctc gtgtaggtgt
     5701 cgcctccgtc cgtggtgatc tgggcgtacc cgaagtcgaa cgtcgtctcc aggtcccagg
     5761 ccgcgtcgaa cgtcagctgt ggcgagcccg ccggcacggt gacgtcccga acgatgacct
     5821 cgttcaggtt gtcaccgacc ggggagtgca gggcggcgcc gtcagcgccg ctcggcggcg
     5881 tcgtgtcgac ctcccaggtg accggcagcg cggggagctc ctgggcaccc tggaactcga
     5941 cgtcggtgac gtcgccggca ctcaggaacg cacctcccgc gtccatcagt cggacgaagt
     6001 cgcccccgtt gggcggcgcg ccgggggtgt cgtaggcgtc gttctccggc gccccggtct
     6061 cccagttgat cgccgcatgg agccgctcga ccgagtacgt ctcgaagtcg cccccgacga
     6121 gggtggctcc gtcgtccagg acggcatcga gcgccaacgt tgcctgccag atgtcgatcg
     6181 cttgcgacat ggtgagcccg gggtccacga cgtccagaac ggcctggatc gcctcgaagc
     6241 cctggttctc gctgtcgttg tggagcgtgg tcaggacctc gtcaccgtag gagtccgcca
     6301 gccacagcag gaagctgtac gcggctccgt agtcgcacag gatctcctgt tcgtgatcga
     6361 agttctggtc gccccagacg ttcagcgagt tctcggggcc acccggcctc gggttcgggt
     6421 tcgcaggtgt ctgcacggcg ttgttgccga gcaagcactg gatgtgcgag tcgaacccga
     6481 gatcggtgat cggcgtggcg gggtcgccgt agccggtcag cgcgatcgcc gtgtccgaga
     6541 gcccctcgtt cacccaggtg gcctctggcg ggtcgacgta ggacaggagc aggtgctggt
     6601 actcgtgggc gaagaccccc tcgtacagag cggggcgtgc cggggcgctc gcgcacgggt
     6661 caccaggcac ggggtcgttc ggcggggtca acccggtacg gtgcagccag tcgaacccgt
     6721 cgatcgtcat cacgttgcgg tcgaagaacc cgttcaagcc ggacgagaag aatccggcga
     6781 tgtaggagaa ctcctgcgtg ttgttgacgt cgtagaagtt gtcgtcacgg acgttgtcga
     6841 tcagtacgac gatgtcgtcg ccgtcgcccg tgtagtagtc ggccgggaga ccgagcgcct
     6901 cggtcgccgg cgcctccgag ccgtcacggt ccggcggcac gctgaagagc tccgactcgg
     6961 tcgggtagat gttgttgtcg aactgatcga tgaagtaccg gatctgggcg ctcgagatca
     7021 tggtccgttc gccgttgcgg caatcgtccc gctggaaccg caggcccgtg gaccggaccc
     7081 cgttgaactt ccggctcccg gccgccgtcc acacctcgat gtgctcaccg acgccacgga
     7141 gccggtagtt cttggtgtag aaggccccgg cgacatcgtc caggccgagc cacgtgcgga
     7201 tctcgccctt ctcggcgagg gccagggtca tggcccgtgc cgtccggctg acggcggcgg
     7261 ccccctgctt ccggatcttc gcccggaggt ccgggggtgt ctgcacgatg tccgaagcgc
     7321 tgccgcgttc cggagcttgc ctggccgacg ttacagtcgc ggccagtccc gtgttggcac
     7381 ccggcacgga taccgccgtg agcgcaacga cgagcgctgc gatgagggtt cgcctcatgc
     7441 aacctcctct catctggtcc ccggatgggg gacggggcct atgagccgga gggtcggagg
     7501 tcgtccgggc gccccggcgg cgttgggcaa cctacccggg ggtttcgaag gccgtcaacc
     7561 gggacccaag cgggggcggt tcgagcctgc ccctcgcggg tagagagcgg agaggacgaa
     7621 agggaggtcg aaatggagac gtgggtgtgg gtggtcgtga tcgccgccgt ggcggtggtg
     7681 gcggtggtcg catggctgct ctcccggcag gccaggagcc gcgggttgca gcgggagttc
     7741 ggctccgagt acgagcgcac gatggctcgc gccggcgaca ggggggtcgc ggaagcagac
     7801 ctcctcgagc ggcgtgatcg ggtgagtgcc ctcgacgtcc gcccactctc gcaggccgat
     7861 cacgatcggt tcgcatccga gtggacgaag gtccaggcgg agttcgtgga cgcgccgtcg
     7921 gaggcggtgg ccgacgcgga cgagctgatc caacaggtga tggcgacgcg cggctacccg
     7981 gtagaggact tcgatcggcg cgcggccgac gtgtcggtcg agcacccgga cgtggtggag
     8041 aactaccggt ccgcgcactc gatcgcggtc aaggaggctc gcgacacgga cggggacacc
     8101 gaagcgctcc gcaaggcgat ggtctactac cgatcgctgt tcgacgagtt gttggtcgtc
     8161 gacgacgcgg gggatcgccg gaactaggag ccgggctcgg gcgcccgacg gtggcgccag
     8221 atccagatcc cgacggtcag gaccacgagg ccgaggccga cccagccgac gcgtttgagg
     8281 atgcggtcga tcgtttccac gtggttgccc acgaggaagc cgagcaaggc gaccgccgtc
     8341 gaccacagga gcatcgtggg gatcgtgaac gcgaagaact tccgtgcggg aacccgggac
     8401 gcgcccgcga tgaacgggat gagccctgct gcgcccgtca cgaaccgtcc gaccacgatc
     8461 gtcttgcccg cgttcgactc gatcgagtcc tccacctggt ccagccgtgc ctcgaatcgc
     8521 cgcaggatcg gcagccgtcg caacagcgag tccccatagc ggcggccgac gagatagccg
     8581 gtcgtttccc cgaggaggcc gaagaaggcc ccgaacgcga tcacggcgcc gaggcccaac
     8641 tcgccttcgg ccgcatagac gcccccgatc gccaagatca cgtcgccggg gacgacgatg
     8701 ccggtcaggg cggccgactc gaagaacatc gcgaccgaga cgatcgcgta cccgaatgtt
     8761 ccgaagaacg ggcggaccca ctcgacgagc tccgtcacaa gcgcgaatcg catcgtgcag
     8821 acagcatggc ggacggtcgg cactactggc atggacccct tccgattggg agtgagccgc
     8881 tccgggaaag gaagagacgc gcaccaagcg gagcgttgct aggagccagg gggagaccga
     8941 tgagcaccgt cgccaccgtc ccggagaccc atcaccaaga tgccggtgac gctcgcgacc
     9001 agctccgacg cagcgggacg ctgcccctcc tccgggacgc cgcgatccgc ctccgcgcgg
     9061 cccatgggac cacctacgcg cgagcactcg gccattcgtc gctcctgacg ctgatcccgg
     9121 cggtgatcgc ggtgatcggc ttcgtcgcgg tcttcgacct cacggggttc gcgcgggtgc
     9181 tccaggacgc cgccacctcg ttcgcgccgg gcccggccgg cagcatcctg acccgggccc
     9241 tcggaaccgc gcgctcctcc gcggggccat ccgccctggt ggtgggtctc gtgggcatgc
     9301 tgttctcggg cacgatcggg atgacgcatc tcgaacgggc agcggatcgg atctacgggg
     9361 tcgaggagga tcggagccct cgggagcgcc tcggcctcgc aatcatgctc gccgcgaccg
     9421 tcgaagtgat gctgatgatc ggcctggcgg tgatcgtggc gggcggctcg atcggcgagg
     9481 ggtcggacgg tggcgccggc tccggcctct ggtccttcgt gcgctggccg gtgggcgtgg
     9541 gtctcgtggc gatcgcgatg acgatcatct tccgcgtggt gccgaacagg caccagcccc
     9601 agttctcgtg gctcctctcc gggacgaccg tgtcggtcac ggcgtgggtc gcggcgacca
     9661 tcctcctcgg cctggtctac gagcacgcgt ccctgctggg cgatgcgttc ggacctctga
     9721 tcggggtggt cgccctgttg gtgtgggcgt acgcgaccgg gcttgccgtc ctgttcggcc
     9781 tcgcgttcgc cgcacagctg gaggccgagc gcgccggcga acacctgaag gcacgggacg
     9841 agccatctcg ataggctcga cgggtccagg ggcggtagct cagttggtca gagcagcgga
     9901 ctcataatcc gtcggtcgca ggttcaagtc ctgcccgccc caccggaaag gcgcaggtca
     9961 gattgtgaag ttccggcacc tctgtcccac atcggctccg gcacctgtgt cccaccctca
    10021 tgcccggctc cggctcccgg gcgacaccgg ctccggcacc tgtgtcccac ccgctccacg
    10081 tgccactcga gcctgatctt ca
//
LOCUS       DASUYD010000036         4239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1122288, whole genome shotgun sequence.
ACCESSION   DASUYD010000036 DASUYD010000000
VERSION     DASUYD010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122288"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..688)
                     /locus_tag="VF195_01920"
     CDS             complement(35..688)
                     /locus_tag="VF195_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579613.1"
                     /translation="MSDAVSAVVVAAHPTVRRVVEIACREAGVIVVDSSGTAADATAA
                     CRSHEPELLVLDLDLPDADGFRVLADLGDLRPRSILVLAERADGDHVLRALRLGARGY
                     LTKAGGLRDLGTTIRRVIAGERVMTPELEKDAIGALGRMARHAREGADLAESLTTRQQ
                     QVLEFLSQGKTVNQIASRLGISPRTVETHVATLYRRLGVNTRVQAVSQAARLGLVDL"
     gene            complement(706..1368)
                     /locus_tag="VF195_01925"
     CDS             complement(706..1368)
                     /locus_tag="VF195_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579614.1"
                     /translation="MDTIRILVVAESLGLAQELLIALRRRSGFDVLGPLSDESAVIEL
                     FAEARPDLVVVDLDRCDGRGLRILAAIRDEASVPAMVATRDTSAGNVELALVAGACGV
                     LPIDRQPASLASSFRRAVAGELVLPADDLSASVGRLREARTRRGRHAALATLTQRERE
                     VITGFARGVSISGIAVELGISRSTVQTHVKNILRKLGVHSTIEAVGVAWRAGLAVDTR
                     SA"
     gene            1441..3444
                     /locus_tag="VF195_01930"
     CDS             1441..3444
                     /locus_tag="VF195_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX6579615.1"
                     /translation="MNRLAGETSPYLLQHAENPVDWSPWDEEALRRARIEDRPIFLSV
                     GYAACHWCHVMERESFEDEGTAAFLNANFVPIKVDREERPDLDAIYMDAVQAMTGQGG
                     WPLSAFLTPDGRPFFAGTYFPKEATHGTPSFRQVLEAIAESWRDRRNDLERQGSGVVE
                     AISRTISVTGSEEPLTDEISMSALAELRSAFDERWGGFGGAPKFPQPMTLEFVLRMAV
                     RGKPDALEMLTTTLDRIASGGIYDQVGGGFARYSTDAAWHVPHFEKMLYDNAQLAQLY
                     VRAWLLTRDDRYRRVATETLDYLLREMHHPEGGFYSSQDADSEGVEGKFFTWTWGELV
                     HLIGADMADAFGATPAGNWESTNVLWLPDGSEPSGLDLSAARRALFEAREARVRPGTD
                     DKVLTAWNAMAITALAEAGRAFGEPRFIEAAEGCARFLVKNLRRADGRLLRSWREGVP
                     GRAGFCDDHALLASACLTLFSTTGTVGWFREAVVLLDALVDLFLDAERGGFFQTGSDD
                     DPLVVRPKELYDNAVPSGNSVAADVLQRVALLSGDPAGEQMGVAAIRLVRDVLGRAPT
                     AFGHALCALDLYLGPSREVAIIGAPRHPATDALVDVVLKDRYLPNTVLAVAAPDDREA
                     AAVPLLRNRPQLHGDPTAYVCQGFVCTRPTTDPATLGRQLDET"
     gene            3527..4036
                     /locus_tag="VF195_01935"
     CDS             3527..4036
                     /locus_tag="VF195_01935"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX6579616.1"
                     /translation="MPSATVHTNKGSFEVRLMSAHAPRTVENFVGLASGSKEWTDPRD
                     GERKTTPLYPGTIFHRVIPGFMVQGGDPTGTGMGGPGYQFEDEVPSDGPAFDRPGLLA
                     MANAGPGTNGSQFFVTVAATPWLDGKHTIFGEVTDGYDVVEEIAGAQTGAQDRPIEDV
                     VIHRIEIHE"
BASE COUNT          717 a         1424 c         1432 g          666 t
ORIGIN      
        1 ccaccttcgc cgttcggagt agtggatcgc aacgctacag gtcgaccaat ccgagtctcg
       61 cagcctggga gaccgcctgt acacgcgtgt tcactccgag ccttcggtac agggtcgcca
      121 cgtgtgtttc cacggtccga ggagagatcc cgagtctcga agcgatctga ttcaccgtct
      181 tcccctggct caagaactcg agcacctgct gctggcgggt cgtgaggctc tccgcgagat
      241 cggccccctc cctcgcgtgc cgggccatcc gacccaacgc accgatggcg tccttctcga
      301 gttccggagt catgacccgt tcaccggcga tgacgcgccg gatggtggtc ccgagatcac
      361 gaagtccccc ggccttggtg agatatccgc gtgcacccaa ccgcagggcc cgcaggacat
      421 gatcgccgtc tgccctctct gccaacacca agatcgaccg tgggcggagg tcaccgagat
      481 cggcgaggac acggaagccg tcggcatccg gcagatccaa gtccagcacg aggagctcag
      541 gctcgtgtga tcgacatgct gcggttgcat cggccgccgt gccgctcgag tcgacgacga
      601 tcactcccgc ctcgcgacac gcgatctcca cgacacggcg gacggtggga tgggcggcga
      661 ccaccacggc actcaccgca tcgctcatgc cgagaggcta gcccctcacg cggagcgcgt
      721 gtcgaccgcg agaccggcgc gccacgccac accgacagcc tcgatcgtcg agtgcacccc
      781 gagcttgcga aggatgttct tgacgtgtgt ctgcaccgtc gatcggctga tcccgagctc
      841 cacagcgatc ccgctgatcg acacgccccg cgcgaagccc gtgatgactt cgcgttcccg
      901 ttgcgtgagg gtggccaaag cggcgtggcg gccgcgccga gtgcgggcct cacgcagtcg
      961 ccccacggag gccgacaggt catccgcggg aaggacgagc tcgccggcga ccgctcgccg
     1021 gaacgagctc gccagactcg ccggctggcg atcgatcggc aggaccccgc acgcccccgc
     1081 aaccagggcg agctccacgt tcccggcgga cgtgtctcgg gtcgcgacca tcgcggggac
     1141 gctcgcttca tctctgatcg ccgccaggat ccggagaccc ctgccgtcgc accgatccag
     1201 gtccacgacc acgaggtcgg gtcgggcctc ggcgaagagc tcgatgaccg ccgactcatc
     1261 tgagagtgga ccgagcacgt cgaagcccga gcgcctgcgg agggcgatca ggagctcctg
     1321 agcgagtccc aagctctcgg cgaccacgag gatcctgatg gtgtccatac gtctacccgg
     1381 aacatcggca tctccgagga tgaagctgag ctgttccgtg agggctggga cactcgtctc
     1441 atgaatcgtc tggcgggaga gacgagtccg tacctgctcc agcacgcaga gaatcccgtg
     1501 gactggtcac cgtgggatga ggaggcactt cggcgcgcac ggatcgagga tcggccgata
     1561 ttcctgtcgg tcggctacgc cgcgtgccac tggtgccacg tcatggagcg cgagtcgttc
     1621 gaggacgagg ggacagccgc cttcctgaac gcgaacttcg tcccgatcaa ggtggaccgg
     1681 gaggagcggc ccgatctcga cgcgatctac atggatgcgg tgcaggcgat gaccggacag
     1741 gggggatggc cgctctcggc attcctcacc cccgacggtc ggccgttctt cgcgggcacc
     1801 tacttcccga aggaagccac ccatggcacg ccatcgttcc ggcaggtcct cgaggcgatc
     1861 gcggagtcat ggagagaccg gcgcaacgac ctcgaacggc aaggaagcgg ggtcgtggag
     1921 gcgatctcga ggaccatatc ggtcaccggt tctgaggagc cgctcaccga cgagatcagc
     1981 atgagcgcct tggccgagct ccggagtgcg ttcgacgagc gatggggagg cttcggcggg
     2041 gcgccgaagt tcccgcagcc gatgacgctg gagttcgtcc ttcggatggc ggtacgcggg
     2101 aaaccggacg cgctcgagat gctgacgacg accttggatc ggatcgcctc aggcgggatc
     2161 tacgaccagg tcggaggcgg gttcgcgcgc tactccacgg acgccgcctg gcacgttccc
     2221 cacttcgaga agatgctcta cgacaacgcg cagctcgcgc agctctacgt ccgggcatgg
     2281 ttgttgactc gcgacgaccg gtatcgccgg gtcgccaccg agaccctcga ctatctcctc
     2341 cgcgagatgc accatccaga gggcggcttc tactcgagtc aggatgcgga ttccgagggt
     2401 gtcgagggca agttcttcac gtggacctgg ggggagctcg tccatctcat cggcgccgac
     2461 atggccgacg ccttcggagc cacgccggcg ggcaactggg agagcacgaa cgtgctgtgg
     2521 ctccccgacg gttcggagcc gagtggcctc gacctctccg cggcgcgacg cgcgctcttc
     2581 gaggcgagag aggccagggt ccggccgggg acggacgaca aggtgctcac cgcgtggaac
     2641 gcgatggcga tcacggccct cgccgaagca ggccgtgcct tcggggagcc ccggttcatc
     2701 gaggcggccg aaggctgcgc gcgcttcctc gtgaagaacc tgcgccgagc cgacgggcga
     2761 ctcctgcgct catggcggga aggcgttccc ggacgggccg gcttctgcga tgaccatgcg
     2821 ctgctggcct cggcctgcct cacactgttc tcgaccacgg gaacggtcgg ctggttccgc
     2881 gaagccgtcg tgctcctgga cgcgttggtg gacctgttcc tcgacgcgga gcgcggtggc
     2941 ttctttcaga cgggatcgga tgacgacccg ctcgtcgtcc gaccgaagga gttgtacgac
     3001 aacgccgtac cctccgggaa ctcggtggcc gccgacgtcc tccagcgggt cgccctcctc
     3061 tccggtgacc ccgccgggga gcagatgggc gtcgcggcga tccggttggt ccgcgacgta
     3121 ttggggaggg caccgacggc gttcgggcac gccctctgtg cgctcgacct ctacctcggc
     3181 ccgtcccgcg aggtggcgat catcggggcc cccaggcacc cggcaacgga cgccctggtg
     3241 gacgtcgtcc tcaaggaccg atatctcccc aacacggtgc tggcggttgc tgcgccggac
     3301 gaccgggaag ccgcggccgt ccccttactc aggaacaggc ctcaactgca tggtgatccg
     3361 accgcctacg tctgtcaggg cttcgtgtgc acccgtccca ccaccgatcc agccacgctc
     3421 gggcggcaat tggacgagac gtagcgagga gcgcgggagc caacgggatc tcccacgctc
     3481 ggtagggtga cgtgacgtcc caccggtcga gagaacggag cgaaccatgc cgagcgcaac
     3541 cgtccacacc aacaagggct cattcgaggt gcggttgatg tccgcacacg cccccaggac
     3601 cgtggagaac ttcgtgggcc tcgcctccgg ttcgaaggag tggacagatc ctcgtgatgg
     3661 cgagcggaag acgaccccgc tgtaccccgg cacgatcttc catcgagtga tccctgggtt
     3721 catggtgcaa gggggtgacc cgacgggcac agggatgggc gggccgggtt atcagttcga
     3781 ggacgaggtg ccgtccgacg ggcccgcgtt cgatcgacca gggctgctcg cgatggcgaa
     3841 tgcagggccg gggacgaacg gttctcagtt cttcgtcacg gtcgccgcga cgccatggct
     3901 cgatgggaag cacaccatct tcggcgaggt cacggacggc tacgacgtcg tggaggagat
     3961 cgccggcgca cagacgggcg cccaggaccg gcccatcgag gacgtggtga tccaccggat
     4021 cgagatccac gagtagcgcc cttcatcccg cggcggccag gtcgataatc accgcgtgcc
     4081 cggagtcacc tccttcgaac tcgaccgcta tcggaacccg gacggggggt tcagccctcg
     4141 cgtgggtcag ccgagcgaac tggagcccac cgcgctcgcc gcgttggcgt tggcagatcc
     4201 ggacgcggtg gcgtggttgg ccgcggcgca gcgatcgga
//
LOCUS       DASUYD010000037        13226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1563209, whole genome shotgun sequence.
ACCESSION   DASUYD010000037 DASUYD010000000
VERSION     DASUYD010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13226
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1563209"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VF195_01940"
     CDS             complement(<1..512)
                     /locus_tag="VF195_01940"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011175887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX6579617.1"
                     /translation="MKRCGWAPVDNASYLAYHDEEWGVPVHDDRRLFEMLTLEGAQAG
                     LSWSTILNKREGYRRAFAGFDPGTVARFSRRKVERLLADPSIVRNRMKVESTIANARQ
                     VLTVQEEDGSLDAYLWSFVGGSPKVNRFRSLGDVPPETAESKAMSKDLKRRGFRFVGP
                     TVCYAFMQACG"
     gene            complement(509..1288)
                     /locus_tag="VF195_01945"
     CDS             complement(509..1288)
                     /locus_tag="VF195_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX6579618.1"
                     /translation="MRDWYRRAVFEKELAFAHEIADRAAEISMSYFLGEFEVRQKPDL
                     SPVTQADLEVETAVREMIAARFPADAITGEEHGEGAGDRVWIVDPIDGTRNFADGVPI
                     WATLLALQVDGRSVLGMASAPALGERYEGVRGGGARWNGRELHASERKLDDAFLAFSS
                     VGDWLGGPREDAFVELVRDTRRSRGFGDFWGHMLVARGAVDLMIEPSLRIWDWASIVV
                     IVEEAGGVVTTFEGEEPSDGASILTSNAVVHDEVVQRLSGR"
     gene            1363..2712
                     /gene="tig"
                     /locus_tag="VF195_01950"
     CDS             1363..2712
                     /gene="tig"
                     /locus_tag="VF195_01950"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX6579619.1"
                     /translation="METTVETTEPHTVKLTIEVPPEEFRKDLDRTYRAIANEIKVPGF
                     RKGKVPKQIIDTQIGRDAIFEQFVNTSVPAYFRQAVTDEDLAPITDPDIDVQQLEPGR
                     SFLFTATVEVRPRLTFEEEDYTGIRVTKPPIEVSDEEIDEWIERLRERFAELEPVGRP
                     VQQGDFVTVDLTVMRGSQKVEQASRVDYLYTVGSGEVGEKLDVELVGTKPGAILKVAD
                     TLPERFGEELGGAPVDISVIVKDVKARRLPEVDDEFAKTASEFDTIPQLRDDLRERLT
                     EVKEREATAALRDLALQAMIEDVDVDLPGSLIEEETEHRIHHARDQAERMGIGLEKML
                     ELQGWDEARLREDSRQHAIRAIKSDLVLEGIARSASLDVTAEEIGAEIAVLAQAYGRE
                     PKAVAKDLERSGQVVTLAGDIIRSKALDLLVERADIETVAPDDAERAPENAQEEANT"
     gene            2712..3353
                     /gene="clpP"
                     /locus_tag="VF195_01955"
     CDS             2712..3353
                     /gene="clpP"
                     /locus_tag="VF195_01955"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009657040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX6579620.1"
                     /translation="MDTPESYLVPMVVEQTSRGERSFDIYSRLLKERIIFLGTPIDDS
                     VANLVMAQMLHLESEDPDKDINLYINSPGGDVASGFAVYDTMQYIKPDITTICMGQAA
                     SFAAFLLLAGAKGKRFMLPHARVLLHQPHGGTQGQAVDIEIQAREILRYRQLLDELIA
                     QHTGQSVDKITKDTDRDFILTSEQAKEYGIIDEVLTNRRDMPAELTAAAAVGA"
     gene            3468..4754
                     /gene="clpX"
                     /locus_tag="VF195_01960"
     CDS             3468..4754
                     /gene="clpX"
                     /locus_tag="VF195_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018634851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX6579621.1"
                     /translation="MAKFGESDLLKCSFCGKSQKQVKKLIAGPGVYICDECIELCNEI
                     IEEELSDTAELKLDELPKPAEIYGFLDEYVVGQDQAKKVMSVAVYNHYKRIQVGAKDQ
                     EVELQKSNILILGPTGCGKTYLAQTLARMLNVPFAIADATALTEAGYVGEDVENILLK
                     LIQAADYDVKKAETGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTTASV
                     PPQGGRKHPHQEFIQIDTTNVLFICGGAFAGLDSIIESRIGRKGVGFGAQVMSRQEKD
                     LGAIFAQVLPEDLLKFGLIPEFVGRLPVLTSVNSLDESALVDILTQPKNALVKQYMKF
                     FEFDGVELEFTDDAMRAISEQAIQRGTGARGLRAIMEEVLLNVMYELPSRQDVTKCLV
                     DRDVVLTGVNPTLVPRAKQASSKKRTGGGERQEKTA"
     gene            4763..5197
                     /locus_tag="VF195_01965"
     CDS             4763..5197
                     /locus_tag="VF195_01965"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEX6579622.1"
                     /translation="MRPPRGRRFESLALPIALVVVVACAGGDDLDGTWRAVEIAGRPV
                     LAGVDATIGFSGGEVSGSSSCNSYTGEYDAEPAFPDGPQDPEISITNVGGTEMACEEP
                     VMDQEARFVQALLSATTYRLDVDRLELYEEEALVAAFERSSG"
     gene            complement(5202..5627)
                     /locus_tag="VF195_01970"
     CDS             complement(5202..5627)
                     /locus_tag="VF195_01970"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6579623.1"
                     /translation="MAAKDALLDRLGSVAIFRGLDEKELHRIVEVGKEVHFEPGAVVA
                     QQDGGAAGFHLIMDGEVSVDVDGHERARLGSGNYFGEMSLLDGLPRSATVKAEQPTTT
                     FALTSWQFLPLLDEYPSISRALLVELCARLRRVEHDPVQ"
     gene            5729..8401
                     /locus_tag="VF195_01975"
     CDS             5729..8401
                     /locus_tag="VF195_01975"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6579624.1"
                     /translation="MANAGTGPGTEMPTRYDAGSVEPRWAETWIERGYFHAEVPEVPA
                     DAYCIVIPPPNVTGRLHVGHALGRTLEDMLVRRARMRGLAALWVPGMDHAGIATQVVV
                     ERELRKEGVERRDIGREAFVERVWEWKERYGGEIVGQIKRMGASLDWDRERFTMDEGL
                     VRAVRVAFVRWFERGLIYRGERLVNWCPNDQTGLSDSEVEHEEVDGELLTFRYPLSDG
                     SGHVEVVTTRVETMLGDTGIAVHPEDVRYRELVGSTVRHPFDGRDLPVVADDAVDPAF
                     GTGAVKVTPAHDPTDFEIARRTGLPLLNIFDAEARINERAAEEFYGLGRYDARQAVRE
                     ELEKLGLLVGVEHPYRHAVGHCYRCHSEIEPWMSGLQWFVAVEDLKRPAIEAALDGRI
                     TFWPERWRKAYVSWMEGLRDWNISRQLWWGHRIPAWYCPEGHVTVALEDPDACATCGS
                     AELEQDPDVLDTWFSSQLWPFSTLGWPEDTPELRAFYPNAALVTGYEILYLWVARMIM
                     SGMSLAGDIPFRDVVIHGLVRDEHGRKMSKSLGNVIDPIEMIDLHGADALRFSLARLA
                     TGGQQDIPLSVDSIKGGRNFANKIWNAARLVLQEFPGGSPELPPEERLTAPQRWLLAR
                     HQRCVMEVNQALEGFEFAPAAQALYRFFWSEFCDWGLEMEKDRLRSEDEGVRADTAHV
                     LAWILERTLRLLHPVMPFVTEEIWQRFGIGETIMRAPWPETEEFRSHESLGADAEEVW
                     PFVEELVTSVRRFRGEHGISPKTRIEIRLVRREHRDPGFGRFEQEILKLAGGSRIELV
                     GPGDASGSPRVVVRGETILLPVGDLIDLDAERDRLRKRLAAAEDERGRAEKKLANAGF
                     RDKAPEDVVRAEQQKVERFEREAAALQEQLAELG"
     gene            8401..9690
                     /locus_tag="VF195_01980"
     CDS             8401..9690
                     /locus_tag="VF195_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6579625.1"
                     /translation="MRFEEAVARLDRRQPEHMPEPSLDRIRAISELLDDPQRTYPTIH
                     VTGTNGKTTIAKAAAEVACAHGLTTGLFTSPHLHTVRERLSLCGVEISEEEFAEEWEH
                     LEPFLDVVDARENGAVTYFEAVTGLAFLWFADKPVALAVVEVGMGGSWDATNLVTGDV
                     AVIGEVGLDHPELGSTIREVATEKAGIIKPGKTVVVRSQPDDALEVIEARAVEVEATV
                     LLESRDWGLATRVPAVGGQQFRVDGLHAAYDDLFLPMFGEHAVLNVVAAMVAVESVLG
                     HALDPDTTRGAIEALRIPGRLEVMSRSPLLVVDGAHNPGGAAALARTLAEAFTWNRMH
                     VVLAVSSNKDLDGVVAALAPIADVWFAARNDNVRSFPPEHVAERIAAAGGRVADQGTV
                     EEGLAWARDAAGPDDLILVTGSLYTVADARRAVEGSA"
     gene            9693..10136
                     /gene="ndk"
                     /locus_tag="VF195_01985"
     CDS             9693..10136
                     /gene="ndk"
                     /locus_tag="VF195_01985"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEX6579626.1"
                     /translation="MTPRDAPRDAPRDAKEATLLIVKPDGVRRHLIGEVLRRVEEAGL
                     TIQDLRMETIARERAEEHYGEHRDKPFYWELVDFITGGPVVLAKVEGDNAIALWRELM
                     GPTDPADAPAGTIRGDLATEITENLVHGSDSPESAARELKLFFGD"
     gene            10144..11793
                     /locus_tag="VF195_01990"
     CDS             10144..11793
                     /locus_tag="VF195_01990"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6579627.1"
                     /translation="MAGLQEAFDRVGASLEHHLTASHAAGAALAVTDREEILGVAVRG
                     MADVASATPVRPETRFQIGSISKSFAAIVAMQEVDGGRLDLHVSVNELLPWLALPEPL
                     GPITMHHLLTHTAGLHTGTEDAPGFAGALQLLREYPATSAPGERFHYSNDGYKIVGAV
                     LEEITRLPIHDLLAERLLGPLGMTSSVAAITDEIWTDHATGYEPMLTDRPAQLRHPLA
                     VAPRIVSNTADGSIVSTVVDMCAYARLLLARGDRPDGRGGRILSEDGFTRLMASAVDD
                     TDHGRYGYGLWTEEVEGRTWFGHSGGMVGYTAFLITLLDEGMACVILQNGGGEKRGVT
                     AAALAAVRAGLAGDELPAPWAPPAATAISPRDDFVGRYAGEDGRTLEVREDEDGLSMS
                     IGSVTARLERDPLVADPGGSFLAAHPAFDRFLLGFGRDEGGRVVEAFHGGTWFRGEVY
                     QGPDPEEPPAGWDRHPGIYRNNDPWSAVLRVVLRKGRLAILWPTDASDEVGGELTPLD
                     DGSFAVGDPAVPLRVRFEGEVRGMTAVVVCNGGRWFRSFEE"
     gene            complement(11807..13078)
                     /gene="fabF"
                     /locus_tag="VF195_01995"
     CDS             complement(11807..13078)
                     /gene="fabF"
                     /locus_tag="VF195_01995"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX6579628.1"
                     /translation="MALTTEVPVPPSRAGRKVVVTGIGPVTPVGTGVEDFWTGLTTGR
                     NGVREIARFATDDLPVKVAGDVHDFDPSLWLDPKEIRRTDRFSHYAIAAGKLAWEDAG
                     TPDVPSERGGIVFGTGIGGIETLLVQHSVLLEKGPGRVSPFMVPALMANAAAGHLAMK
                     FGLTGPNYCTVSACASSNHAIGEGMRLIRDGYLDVCITGGSEAATLPLTVAAFAQMTA
                     LTKNPDPESASRPFDANRNGFVLSEGAASLILESEEHARARGARIYCEVAGYGMSDDA
                     HHITAPDPKGSGAALAMRWALADAGEEPASVRYVNAHGTSTPLNDASETVAIKAVLGD
                     EIAHEVAISSTKSMTGHMLGAAGAVEGAVCALAIQRGVVPPTIHYETPDPDCDLDYIP
                     NEARELDVTLALSNSFGFGGQNACVAFRRVA"
     gene            complement(13086..13211)
                     /locus_tag="VF195_02000"
     CDS             complement(13086..13211)
                     /locus_tag="VF195_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579629.1"
                     /translation="MVEAVLALEEEWSIEIPEDEMESVKTVGQAVDLVASKLGMS"
BASE COUNT         2172 a         4440 c         4658 g         1956 t
ORIGIN      
        1 ccgcaggcct gcatgaacgc gtagcagacg gtcggtccga cgaaccggaa cccgcgccgc
       61 ttcaggtcct tcgacatggc cttcgactcc gcggtctccg gggggacgtc cccgaggctg
      121 cggaaccggt tcaccttcgg cgagccgcct acgaaggacc acagatacgc gtcgagtgag
      181 ccgtcctcct cctggacggt gagcacctgt cgcgcgttcg cgatcgtcga ctccaccttc
      241 atgcggttgc ggacgatcga gggatccgcg aggagccgct cgaccttccg cctgctgaag
      301 cgcgccacgg tgcccgggtc gaatcccgcg aacgcgcgac ggtagccctc gcgtttgttc
      361 aggatcgtgg accacgacag cccggcctgc gcgccttcca gcgtcagcat ctcgaagagc
      421 cgccggtcgt cgtggaccgg gacgccccat tcctcgtcgt ggtaggcgag gtaggaggca
      481 ttgtccacgg gcgcccaccc gcagcgcttc accggcccga gaggcgttgg acgacctcgt
      541 cgtgcacgac cgcgttggac gtgaggatgc tcgcgccgtc cgacggctcc tcgccctcga
      601 acgtggtgac cacgccgccg gcttcctcga cgatcacgac gatcgaggcc cagtcccaga
      661 tccgcagaga cggctcgatc atcagatcca cggcgccgcg cgccaccagc atgtgccccc
      721 agaagtcccc gaaccctcgg ctgcggcgcg tgtcgcggac gagttcgacg aaggcgtcct
      781 cgcgaggccc gccgagccag tcgccgacgc tcgagaacgc caggaacgca tcgtcgagct
      841 tccgctcgct cgcgtggagc tcgcggccgt tccatcgggc gccgccgccg cgaacgccct
      901 cgtaccgttc gccgagcgcc ggcgcgctcg ccatgccgag caccgatcgc ccgtccacct
      961 ggagggcgag cagcgtcgcc cagatcggga cgccgtcggc gaagttcctc gtgccgtcga
     1021 tcggatcgac gatccagacg cggtcgccgg cgccctcgcc gtgttcctcc ccggtgatcg
     1081 cgtcggccgg gaaccgcgcg gcgatcatct ccctgacggc ggtctccacc tcgaggtccg
     1141 cctgggtcac gggagacaga tccggcttct gcctgacctc gaactccccg aggaagtacg
     1201 acatcgagat ctccgccgcc cggtcggcga tctcgtgcgc gaacgcgagc tccttctcga
     1261 acacggcccg acggtaccag tcgcgcacgg ggcgctcttc cgatgtcgac cgacaccgcc
     1321 gagagccgag tgtcaccccg ggtcgagtag cctgggtcct caatggaaac gaccgtcgaa
     1381 accaccgaac cgcacaccgt gaagctcacg atcgaggtgc cccccgagga gttccgcaag
     1441 gacctcgacc gcacgtaccg agcgatcgcc aacgagatca aggtccccgg gttccgcaag
     1501 ggcaaggtgc cgaagcagat catcgacacc cagatcggcc gggacgcgat cttcgagcag
     1561 ttcgtgaaca cgtccgtgcc ggcctacttc cggcaggccg tgacggacga ggatctcgcg
     1621 ccgatcaccg atcccgacat cgacgtgcag cagcttgagc cgggtcggtc cttcctgttc
     1681 accgccaccg tggaggtccg cccccgcctc accttcgagg aagaggacta cacggggatc
     1741 cgcgtcacga agccgccgat cgaggtgagc gacgaagaga tcgacgaatg gatcgagcgc
     1801 ctccgggaac ggttcgcgga gctcgagccg gtcgggaggc ccgtccagca gggcgacttc
     1861 gtgaccgtgg acctgaccgt gatgaggggg agccagaagg tcgaacaggc ctcgcgtgtc
     1921 gactacctct acaccgtggg ctccggcgag gtgggggaga agctcgacgt ggagctcgtg
     1981 ggcacgaagc ccggcgcgat cctgaaggtg gccgacacgc tgccggagcg cttcggcgag
     2041 gagctgggcg gcgcgccggt cgatatcagc gtgatcgtga aggacgtgaa ggcgcggcgt
     2101 ctccccgagg tggacgacga gttcgccaag acggcctcgg agttcgacac gatcccccag
     2161 ctccgcgacg acctgcgcga gcggctcacc gaggtgaagg agcgcgaggc caccgccgcg
     2221 ctccgcgatc tggccctgca agcgatgatc gaggatgtcg acgtcgatct gcccggcagc
     2281 ttgatcgagg aggagaccga gcaccggatc catcacgcgc gcgaccaggc ggagcggatg
     2341 gggatcggcc tcgagaagat gctcgagctg cagggatggg acgaggcgcg tcttcgcgag
     2401 gactcccgcc agcacgcgat ccgggcgatc aagagcgacc tcgtcttgga aggcatcgcc
     2461 cgcagcgcat ccctggacgt cacggcggag gagatcggag cggagatcgc cgtcctcgcg
     2521 caagcctacg gcagggagcc gaaggcagtc gccaaggatc tcgagcgcag cggtcaggtc
     2581 gtaacgctgg ccggggatat catcaggagc aaggccctcg atctcttggt cgaacgtgcc
     2641 gatatcgaga cggttgcacc ggacgacgcc gaacgcgcgc cagagaacgc acaagaggag
     2701 gcgaacacgt gatggacacc cctgagagct acctggtccc gatggtggtc gagcagacca
     2761 gccgaggcga gcgctcgttc gacatctact cgcggttgct gaaggaacgg atcatcttcc
     2821 tcggcacgcc gatcgacgac tcggtggcga acctcgtgat ggcgcagatg ctccatctcg
     2881 agtccgagga tccggacaag gacatcaacc tgtacatcaa ctcgccgggt ggtgacgtcg
     2941 cgtcgggctt cgccgtctac gacacgatgc agtacatcaa gcccgacatc acgacgatct
     3001 gcatgggcca ggccgcgtcg ttcgcggcgt tcctcctact cgccggcgcc aagggcaagc
     3061 gattcatgct cccgcacgca cgggtgctgc tccaccagcc ccacggcggg acgcagggcc
     3121 aggccgtcga catcgagatc caggcccgcg agatcctgcg gtaccggcag ctcctcgacg
     3181 agctgatcgc acagcacacc ggccagtccg tcgacaagat cacgaaggac acggaccggg
     3241 acttcatcct gacctcggag caggcgaagg agtacgggat catcgacgag gtcctgacga
     3301 accgtcgcga tatgccggcg gagttgacgg cggcggccgc cgtaggggcg tagaatccac
     3361 cccacgaccg ggcgatgccc ggtcggtggt gtctacgaga gcagcacaag gagtcgcggc
     3421 caccaagaac ggtccaccaa gaaccggcca ccaggaaagg gagtcggatg gcgaagttcg
     3481 gcgagtcgga cctgctgaag tgctccttct gcgggaagag ccagaagcag gtcaagaagc
     3541 tgatcgccgg tccgggcgtc tacatctgcg acgagtgcat cgagctctgt aacgagatca
     3601 tcgaggagga gctctccgac accgccgagc tgaagctcga cgagctgccg aagcccgcgg
     3661 agatctacgg cttcctcgac gagtacgtgg tggggcagga ccaggcgaag aaggtcatgt
     3721 cggtcgccgt ctacaaccac tacaagcgga tccaggtggg cgcgaaggat caggaggtcg
     3781 agctccagaa gtcgaacatc ctgatcttgg ggccgacggg ctgcggcaag acctacctgg
     3841 cgcagacgct cgcccggatg ttgaacgtgc cgttcgcgat cgccgacgcc acggcgctca
     3901 cggaggccgg ttacgtcggt gaggacgtcg agaacatcct gctgaagctg atccaggccg
     3961 ccgactacga cgtgaagaag gccgagacgg gcatcatcta catcgatgag atcgacaaga
     4021 tcgcccgcaa gtccgagaac ccctccatca cgcgcgacgt gtccggcgaa ggggttcagc
     4081 aggcgctcct gaagatcctc gaggggacca ccgcctccgt gccgccgcag gggggccgca
     4141 agcatcccca ccaggagttc atccagatcg acaccacgaa cgtcctgttc atctgcgggg
     4201 gcgcgttcgc cggtctcgac tccatcatcg agagcaggat cgggcgcaaa ggggtcggct
     4261 tcggggctca ggtcatgagc cggcaggaga aggacctcgg tgcgatcttc gcccaagtgc
     4321 tccctgagga cctgttgaag ttcgggctga tccccgagtt cgtcggtcgc ctcccggtgc
     4381 tgacgagcgt gaacagcctc gacgagtcgg cgctggtcga catcctcacg cagccgaaga
     4441 acgcgctcgt gaagcagtac atgaagttct tcgagttcga cggcgtcgag ctcgagttca
     4501 ccgacgacgc gatgcgggcg atctcggagc aggcgatcca gcggggcacc ggggcccgcg
     4561 gcctccgggc gatcatggag gaggtcctcc tgaacgtgat gtacgagctg ccgtcgcggc
     4621 aggacgtcac gaagtgcctc gtcgaccgcg acgtcgtgct gaccggtgtg aatcccaccc
     4681 tggtcccgcg cgcgaagcag gcgtcgtcga agaagcggac cggcggcggc gagcgccagg
     4741 agaagacggc ctaggacctc gcgtgcgtcc tccccgcgga cgacgcttcg aatcgctcgc
     4801 cctcccgatc gcccttgtcg tcgtcgtcgc ctgtgcgggc ggggacgacc tcgacgggac
     4861 gtggcgagcc gttgagatcg cggggcggcc ggtcctcgcc ggcgtcgacg cgaccatcgg
     4921 gttctcgggc ggcgaggtct ccggttccag cagctgcaac tcgtacacgg gcgagtacga
     4981 cgctgagccg gcgttccccg acggtccgca ggatcccgag atctcgatca cgaacgtcgg
     5041 cggcaccgag atggcatgcg aggagccggt gatggaccag gaggcgaggt tcgtgcaggc
     5101 gttgctgagc gcgaccacgt accgactcga cgtcgacagg ctcgagctct acgaggaaga
     5161 ggcgctcgtc gccgcgttcg agcggtcgag cggctagtcg actactggac cggatcgtgc
     5221 tccacgcgcc ggagccgcgc acagagctcc accaggagcg cgcggctgat cgaggggtat
     5281 tcgtcgagca gcggcaggaa ctgccatgac gtcagggcga acgtggtcgt cggctgctcc
     5341 gccttcacgg tcgccgaccg ggggagcccg tcgagcaacg acatctcgcc gaagtagttg
     5401 ccgctgccga gccgggcgcg ctcgtggccg tccacgtcga ccgagacctc gccgtccatg
     5461 atcaggtgga acccggcagc cccgccgtcc tgttgggcca cgaccgcgcc cggctcgaag
     5521 tggacctctt tgccgacttc gacgatccgg tgaagctcct tctcgtccag ccctctgaag
     5581 atcgccaccg agccgagccg atcgagcaac gcgtccttcg cagccatcga acctcctcgg
     5641 gtgcccgtcg cgcgagtata ggcacagcgc ctccgccggg ttgctcgcgt cctcgggcgc
     5701 gcggaggtgg gtcgatacac tcgatcgcgt ggccaacgcg ggaacgggtc ccgggacgga
     5761 gatgcccacg cggtacgacg ccggctcggt cgaaccgcgg tgggccgaga cgtggatcga
     5821 gcgcggctac ttccacgccg aggtccccga ggtccccgcc gacgcctact gcatcgtgat
     5881 cccgccaccg aacgtcacgg gccggctcca cgtcggccac gccctcgggc ggacgctcga
     5941 ggacatgctc gttcggcgcg cccggatgcg cggcctcgca gccctctggg tgcccgggat
     6001 ggatcacgcg gggatcgcga cacaggtcgt ggtcgaacga gagctgcgga aggaaggcgt
     6061 cgagcggcgc gacatcggcc gagaggcgtt cgtcgagcgc gtctgggagt ggaaggagcg
     6121 gtatggcggc gagatcgtag gacagatcaa gcggatgggc gcctcgctcg actgggaccg
     6181 ggagcgcttc acgatggacg agggtctcgt gcgggcggtc cgcgtcgcgt tcgtccgctg
     6241 gttcgagcgc ggcttgatct atcggggtga gcgtctcgtg aactggtgtc ccaacgacca
     6301 gaccgggctg tcggactccg aggtggagca cgaggaggtc gacggagagc tcctcacgtt
     6361 ccgctacccg ctctccgacg ggagcgggca tgtggaggtc gtgacgaccc gcgtcgagac
     6421 gatgctcggc gacaccggca tcgccgtaca tccggaggac gttcgctacc gggagctagt
     6481 gggcagcacc gtgcgccatc ccttcgacgg ccgcgacctc ccggtcgtcg ccgacgacgc
     6541 ggtcgatccc gcgttcggca ccggcgcggt gaaggtcacg ccggcgcacg accccaccga
     6601 cttcgagatc gcccgacgca ccggactgcc gctgctgaac atcttcgacg cggaggcgcg
     6661 gatcaacgag cgcgcggcgg aggagttcta cgggctcggg cgctacgacg cccggcaggc
     6721 cgtccgcgag gagctcgaga aactcggcct gctcgtcggt gtcgagcacc cctatcggca
     6781 cgcggtcggc cactgctacc gctgtcacag cgagatcgag ccgtggatgt ccgggctgca
     6841 gtggttcgtc gcggtggagg atctgaagcg ccccgcgatc gaggcggcgc tcgacggccg
     6901 gatcaccttc tggccggagc gatggcggaa ggcctacgtg agctggatgg aggggctgcg
     6961 cgactggaac atctcgcgcc agctctggtg gggccaccgc atccccgcgt ggtactgccc
     7021 ggaggggcac gtcacggtgg ccctcgagga tcccgacgcg tgcgccacgt gtgggtcggc
     7081 ggagctcgaa caggatccgg acgtgctcga cacgtggttc tcctcccagc tgtggccgtt
     7141 ctccacgctc ggctggccgg aggacacgcc agagctccgg gcgttctacc cgaacgccgc
     7201 cctcgtcacg ggctacgaga tcctctacct ctgggtcgcg cggatgatca tgtccggcat
     7261 gtcgctggcc ggcgacatcc cgttccgcga cgtcgtgatc cacgggctcg tgcgcgacga
     7321 gcacggccgc aagatgtcga agtcgctcgg gaacgtgatc gatccgatcg agatgatcga
     7381 tctgcacggc gcggatgcgc tgcggttctc gctcgcgcgg ctcgcgaccg gcggccaaca
     7441 ggacatcccg ttgtcggtcg attcgatcaa gggtgggcgc aacttcgcga acaagatctg
     7501 gaacgccgct cggctcgtcc tgcaggagtt ccccggcggt tcgcccgagc tccctccgga
     7561 ggagcgcctg acggccccgc agcgatggct tctggcacga catcagcggt gcgtcatgga
     7621 ggtgaaccag gcgttggagg ggttcgagtt cgcgcccgcc gcccaggccc tctatcgctt
     7681 cttctggtcg gagttctgcg actggggact cgagatggag aaggaccggc tgcgttccga
     7741 ggacgagggg gtgcgggccg acaccgccca cgtcctcgcg tggatcctcg agcggaccct
     7801 gcggctgctg caccccgtga tgccgttcgt caccgaggag atctggcagc ggttcgggat
     7861 cggggagacg atcatgcggg caccctggcc ggagacggag gagttccgga gccatgagtc
     7921 gctcggggcc gatgcggaag aggtgtggcc gttcgtcgag gagctcgtga cctcggtgcg
     7981 gcgcttcaga ggcgaacacg ggatctcgcc gaagacccgg atcgagatcc gactcgtccg
     8041 acgtgagcac cgtgatcccg gcttcggccg cttcgagcag gagatcctca agctcgcggg
     8101 gggctcgcgg atcgagctcg ttggcccggg cgacgcgagc ggttcccccc gggtcgtggt
     8161 ccggggcgag acgatcctgc taccggtcgg agatctgatc gacctggacg cggagcggga
     8221 ccggctgcgc aagcggctcg cagccgccga ggacgagcgc ggtcgcgccg agaagaagct
     8281 cgcgaacgcg ggattccggg acaaggctcc cgaggacgtc gtccgggcgg aacaacagaa
     8341 ggtcgagcgc ttcgagcggg aagctgccgc gctgcaggag cagctcgccg agctcggctg
     8401 atgcggttcg aggaggcggt cgcgcggctg gaccggcggc aaccggagca catgccggag
     8461 cccagcctcg atcggatccg cgccatctcg gagctgctcg acgacccgca gcgcacgtat
     8521 cccacgatcc acgtgacagg tacgaacggg aagacgacga tcgccaaggc cgcggccgag
     8581 gtcgcctgcg cccacggcct caccacgggt ctgttcacct caccgcacct gcacaccgtg
     8641 cgcgaacgcc tctcgctctg tggtgtggag atctccgagg aggagttcgc agaggagtgg
     8701 gagcacctcg agccgttcct cgacgtggtg gacgcacggg agaacggggc ggtgacgtac
     8761 ttcgaggccg ttacggggct cgcgttcctc tggttcgcgg acaaacccgt tgcactggcg
     8821 gtcgtcgagg tcggcatggg cgggtcgtgg gacgcgacga acctcgtcac cggcgacgtc
     8881 gccgtgatcg gcgaggtcgg gctcgatcac ccggagctcg ggtcgacgat ccgtgaggtc
     8941 gccacggaga aggcggggat aatcaaaccc ggcaagaccg tcgtcgtccg atcacagccc
     9001 gacgatgcgc tcgaggtgat cgaagcgcgc gccgtcgagg tagaggcgac cgtcctgctc
     9061 gagagccgcg actgggggct cgccacgcgt gtgccggccg tgggcggcca acagttccgg
     9121 gtcgacggcc tgcacgccgc ctacgacgac ctgttcctcc cgatgttcgg cgagcacgcg
     9181 gtgctgaacg tggtcgccgc gatggtggcg gtcgagtccg tcttggggca cgcgctcgac
     9241 ccggacacca cacgcggcgc gatcgaggcg ctccggatcc ccggccggct cgaggtgatg
     9301 tcccggtcgc cgctgctcgt cgtggacggc gcccacaacc cgggcggcgc ggcggcactc
     9361 gcgcggacgc tcgcggaggc gttcacctgg aaccggatgc acgtggtcct cgccgtctcg
     9421 tcgaacaaag acctcgacgg cgtggtggcc gcgctcgcgc cgatcgccga cgtctggttc
     9481 gcggccagga acgacaacgt gcggagcttc ccccccgagc acgtcgcgga acggatcgcc
     9541 gcggccgggg gtcgggttgc cgaccagggg accgtggagg aagggctcgc gtgggcccgc
     9601 gacgcggccg gccccgacga cctcatcctg gtgacgggct ccctctacac tgtcgcggac
     9661 gcccgccggg cggtggaagg atccgcctga ccatgacccc gagggacgcg ccaagagacg
     9721 cgccgagaga cgccaaggaa gccacgctgc tgatcgtcaa gccggacggc gtccgccggc
     9781 atctgatcgg agaggtgctc cgccgggtcg aggaagccgg gctgacgatc caggacctgc
     9841 ggatggagac gatcgcgcgg gaacgcgccg aggagcacta cggcgagcac cgcgacaagc
     9901 cgttctactg ggagctcgtc gacttcatca ccggtggtcc ggtggtcctg gccaaggtcg
     9961 agggagacaa cgcgatcgcg ttgtggcgcg agctgatggg cccgacggac ccggcggacg
    10021 cacccgccgg cacgatccgc ggcgacctcg cgaccgagat cacggagaac ctcgtgcacg
    10081 gcagcgactc tccggagtcg gcggctcgag agctcaagct cttcttcggc gactgacagc
    10141 gggatggcgg ggctccagga agcgttcgac cgcgtcggtg cgagcctcga gcaccacctc
    10201 accgcctcgc acgccgccgg ggcggcgctg gccgtcacgg atcgcgagga gatcctgggg
    10261 gtcgctgtcc gggggatggc ggacgtcgcg tcggccaccc cggtccgtcc ggagacgcgg
    10321 ttccagatcg gctcgatctc gaagtcgttc gcggcgatcg tcgcgatgca ggaggtggac
    10381 ggggggaggc tggacctgca cgtctccgtg aacgagctgc tcccgtggct cgcgctcccc
    10441 gagccgttgg gtccgatcac gatgcatcac ctgctcacgc acacggcggg gcttcacacc
    10501 gggacggagg acgccccggg attcgcgggc gcgctccaac tgcttcggga gtacccggcc
    10561 acgtcggcgc ccggcgaacg gttccactac tcgaacgacg gctacaagat cgtcggcgcg
    10621 gtgctcgagg agatcacccg cctcccgatc cacgacctcc tcgcggagcg gttgctgggc
    10681 ccgctcggca tgacgtcatc tgtggccgcg atcaccgacg agatctggac ggaccacgcg
    10741 acggggtacg agccgatgct gaccgaccgg ccggcgcagc tgcgtcatcc gctggcggtg
    10801 gccccgcgga tcgtgtcgaa cacggcggac ggctcgatcg tctcgaccgt ggtcgacatg
    10861 tgcgcctacg cacggctcct gctcgctcgt ggcgaccgcc ccgacggccg cgggggccgg
    10921 atcctctccg aggacggctt cacgcgcctg atggcgagcg ccgtcgacga caccgatcac
    10981 gggcggtacg ggtacggcct gtggaccgag gaagtcgagg ggagaacctg gttcggtcac
    11041 tcgggcggca tggtcggcta caccgcgttc ctgatcacgc tcctcgacga ggggatggcg
    11101 tgcgtgatcc tgcagaacgg aggcggggag aagcgcggtg tgacggcagc cgcgttggcg
    11161 gcggtgcggg cgggcctggc gggcgatgag ctcccggcgc cgtgggctcc gcccgcggcc
    11221 accgcgatct ccccgagaga cgacttcgtc ggcagatacg ccggtgagga cggtcgaacg
    11281 ctcgaggttc gggaggacga ggacggtctg tcgatgtcga tcggctcggt gacggctcgc
    11341 ctcgagcggg atcccctcgt cgcggatccc ggtgggtcct tcctggccgc gcatccggcg
    11401 ttcgatcggt tcctcctcgg attcggtcgc gacgaaggag gaagggtcgt cgaggcgttc
    11461 cacggaggca cctggttccg cggcgaggtg taccaggggc ccgaccccga ggagccgccg
    11521 gcagggtggg accggcaccc ggggatctac cgcaacaacg acccctggag cgcggtgctc
    11581 cgtgtcgtcc tccggaaggg ccggctggcg atcctctggc ccaccgatgc gagcgacgag
    11641 gtgggggggg agctcacgcc gctcgacgac gggtcgttcg ctgtcggcga tcccgcggtc
    11701 ccactccgcg tccgcttcga aggggaggtc cgcggcatga cggcggtggt cgtgtgcaac
    11761 ggaggacgat ggttccgctc cttcgaggag tgaccgggcc cgatcctcag gcgacgcggc
    11821 ggaacgccac gcacgcgttc tgtcccccga acccgaacga gttcgacaga gccagggtga
    11881 cgtccagctc gcgggcctcg ttcgggatgt agtcgaggtc gcagtcggga tccggcgtct
    11941 cgtagtggat cgtgggtggg acgacaccac gctggatcgc gagcgcgcag accgcgcctt
    12001 cgaccgcccc cgcggccccg agcatgtggc cggtcatcga cttcgtcgac gagatcgcca
    12061 cctcgtgggc gatctcgtcg ccgagcaccg ccttgatcgc gaccgtctcg ctcgcgtcgt
    12121 tcagcggcgt ggaggtcccg tgcgcgttca cgtaccggac cgacgccggc tcctcgccgg
    12181 cgtcggcgag cgcccagcgc atcgcgagcg ccgcaccgga gcctttcgga tccggcgcgg
    12241 tgatgtggtg ggcgtcgtcg gacatcccgt agcccgcgac ctcgcaatag atccgcgcgc
    12301 cgcgcgcccg cgcgtgctcc tcggactcga ggatcagcga ggccgccccc tccgacagca
    12361 cgaagccgtt gcggttcgcg tcgaacggac gggacgcgct ctcgggatcg gggttcttcg
    12421 tgagcgcggt catctgggcg aacgcggcca ccgtgagtgg gagcgtcgcg gcctcggatc
    12481 ccccggtgat gcacacgtcc aggtaaccgt cgcggatcaa ccgcatccct tccccgatcg
    12541 cgtggttgga ggaggcgcag gcggagacgg tgcagtagtt cggaccggtg aggccgaact
    12601 tcatcgcgag gtgacccgcc gccgcgttgg ccatcagggc ggggaccatg aacggcgaca
    12661 cgcgccccgg gcccttctcc agcaacacgc tgtgctggac aaggagcgtc tcgatcccgc
    12721 cgatccccgt cccgaagacg atgcctcctc gctcggacgg cacgtcgggc gtgcccgcgt
    12781 cctcccatgc gagcttcccc gcggcgatcg cgtagtgcga gaagcgatcg gtccgccgga
    12841 tctccttggg gtcgagccag agcgacgggt cgaagtcgtg gacgtcaccc gcgaccttca
    12901 cgggaaggtc gtccgtggcg aaccgcgcga tctcccgaac cccgttgcgc ccggtcgtca
    12961 gccccgtcca gaagtcctcg acgcccgtgc ccaccggtgt gaccgggccg atgccggtga
    13021 cgacgacctt acggccggcg cgcgatggcg ggacgggcac ttcggtcgtg agcgccatga
    13081 acggcctacg acatccccag cttgctcgcg acgaggtcga cggcctggcc gaccgtcttc
    13141 acggactcca tctcgtcctc ggggatctcg atgctccatt cctcttcgag ggcgagcacc
    13201 gcctcgacca ggtcgaggga gtcggc
//
LOCUS       DASUYD010000038        13258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_682108, whole genome shotgun sequence.
ACCESSION   DASUYD010000038 DASUYD010000000
VERSION     DASUYD010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13258
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682108"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /gene="acpP"
                     /locus_tag="VF195_02005"
     CDS             complement(<1..231)
                     /gene="acpP"
                     /locus_tag="VF195_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015123555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX6579630.1"
                     /translation="MDRAEIETRVKKVLAEQLAVDEQQVVPDARFAEDLNADSLDLVE
                     AVLALEEEWSIEIPEDEMESVKTVGQAVDLVAS"
     gene            complement(363..1100)
                     /gene="fabG"
                     /locus_tag="VF195_02010"
     CDS             complement(363..1100)
                     /gene="fabG"
                     /locus_tag="VF195_02010"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX6579631.1"
                     /translation="MTPKVAMISGASRGIGRACSEALAAAGWTVAVGYRTREADAKET
                     LEALESAGTPGMTVYLDVTDESSVQEAFRRVNDEVGPVTGLVNNSGYSRDGLLLKYSM
                     VEYERTMTTNLRGSFLCSRAALRSMLREKWGRIVNMSSAVAIHGNAGQTVYAATKAGL
                     LGLTKSLAREVGGKGITVNALCPGLLQTEMTAHLTDDARAFYLDQTPVRRAATLEEVS
                     GVVRFLMSEEASYVNGTVIPVDGGLTA"
     gene            complement(1097..2095)
                     /locus_tag="VF195_02015"
     CDS             complement(1097..2095)
                     /locus_tag="VF195_02015"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX6579632.1"
                     /translation="MTRSATITGVGSTLPPRLVPNTWFEDKIDTNDEWIRERTGIEAR
                     HFVEEGVDTSDLVVDAARAAMTTAAITPDQLDMIVVASVTGDTPFPATAVWAQEKLGV
                     RVPAFDVNAACAGFSYGLASATAFVQAGMADTVLLAGAEIFSRILDFTDRTTSILFGD
                     GAGAVIVQASDRPGIEGTVLGADGTAADILQMPAGGTRMPASHATVDARDHSIRMPKG
                     REVFKRAVTEMAAACRELLEKSEHTPADVDVLIPHQANSRIMVAVAERLGVPPERSVL
                     DVAEVGNTSAASIPIALARAWNAGRIKEGDLVLFTSFGAGLTWGATLVRWTMQGPE"
     gene            complement(2135..3064)
                     /locus_tag="VF195_02020"
     CDS             complement(2135..3064)
                     /locus_tag="VF195_02020"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX6579633.1"
                     /translation="MAHLPAVAVLFPGQGSQYPGMADPWIGHPSGRSILQEASDAMGR
                     DIVVGCHDDDALATTEFVQPALLACDVAAFHVLEAEGLAPVGVAGHSLGQFAALVAAD
                     VLSLADALDLVVIRGAAMQRAGEERPGAMTALLGVGAEDAASLCDEVRGGDDVVVANE
                     NSPVQVVISGSVAAIERAETLAKERRIRAVRLPVAGAFHSPLMEPARAAIEERIDAIG
                     FSAPRCPVAENVAGALVSDPDELRSLLTRHVVSPVRWASSILALAEAGAGAFLEAGPG
                     DVLTKLMKRILPDAVARAVGSPDDARATASGTA"
     gene            3273..4640
                     /locus_tag="VF195_02025"
     CDS             3273..4640
                     /locus_tag="VF195_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009944449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6579634.1"
                     /translation="MASTVEELRGRTTGQVITQDDPGYDEARAVYNAMIDKRPSVVVR
                     ATEAADVVAAVNHARENGLDVAVRGGGHSVPGFGTVDDGVVIDLSGMRAVTVDPSSKT
                     ASAQGGATWGDFNDATNAHGLATTGGIISTTGVAGLSLGGGIGYLARGAGLSCDNLLS
                     AVVVTADGQTVTANATEHEDLYWALRGGGGNFGIATSLEFRLHPVAEIYGGPMFFEVA
                     DAGDVLRWYREFIADAPEEFGGFPAWQIAPPLPFVPEERHGDPFLAFVACWAGPVEQG
                     EQMLKPLHDVAPVVAEHVGPMPYPALNSAFDGLVPPGLQHYWKANFVKELTDDAIAAH
                     LDHGPNVPVVNSTVHIYPINGACHRVASDETAFAYRDATFATVIAGMWPDPADNEKNT
                     AWVRDYYGATAPLSEEGGYINFMAEDDQDRIRANYRGNYDRLVEVKRAYDPGNLFRQN
                     QNIVP"
     gene            complement(4582..5661)
                     /locus_tag="VF195_02030"
     CDS             complement(4582..5661)
                     /locus_tag="VF195_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579635.1"
                     /translation="MSGLFAASYPERTRALILYGTFARLEPTTGLLARLADSPEVALD
                     RVEKEWGHASVGLDPWAPSALGDELLADSYLRLLRSSMSPSSARGMMELGFQVDWTAS
                     LPAIRVPTLVLHRRGDLIVPFAHGRALADGIAGSTFVELPGVDHLMWVGDQTTIVNEV
                     RAFIGGLAPARRLDRKLATILFTDIVGSTQTAVRLGDRAWRTLLQEHHRVVRSQIAAS
                     DGHEVETAGDGFLVTFDVPATGIRCARSIIEATSTLGLELRTGIHTGECEVSEDGIQG
                     VAVHTAARVAALASPHEILVSRTVRELSSGAGIRFEDRGTHDLKGIPGRWELFSAEPD
                     GAHRQPLTGRCSGSGGTGFPGRRRA"
     gene            complement(5613..5990)
                     /locus_tag="VF195_02035"
     CDS             complement(5613..5990)
                     /locus_tag="VF195_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579636.1"
                     /translation="MERKVRYAKAGAFNIAYQVVGDGPVDLVLSPGWATHLELAWDIP
                     PLARLLNDLASFSRLILFDKRGTGLSDRFSPDSLPTLEERMSDLRAVMDAAGPSMRHS
                     SARSVAERCPACSRPPTRNGPGP"
     gene            6147..7169
                     /locus_tag="VF195_02040"
     CDS             6147..7169
                     /locus_tag="VF195_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018225978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX6579637.1"
                     /translation="MGFLGRDMAVDLGTANTLVYVRGRGVVLNEPSVVAINTLTGAIL
                     AVGAEAKRMIGRTPSHIQAVRPLKDGVIADFDVTEKMLRYFIQRVHKRRLLAKPRVVV
                     CVPSGITGVEQRAVEEATIAAGARRAYIIEEPMAAAIGAGLPIHEPTGNMVVDIGGGT
                     TEVAVISLGGVVTSTSIRIGGDELDESIIQHVKKEYSLLLGERTSEAIKLAIASVYPM
                     PEELVAEIKGRDLISGLPKTITIAAEEIRRAIEEPVNAIIDAIKTTLDRTPPELAADI
                     MDRGMVLTGGGALLRGLDKRLRHETGMPVHIADTPLQAVVLGSGKCLEEFEVLQRVLV
                     TSTSRR"
     gene            7179..8054
                     /gene="mreC"
                     /locus_tag="VF195_02045"
     CDS             7179..8054
                     /gene="mreC"
                     /locus_tag="VF195_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX6579638.1"
                     /translation="MAIRQRTRSARLLVVTLVAASLAIITVDYRGGDDGPLDSVGEAI
                     SSALAPMQRAVSGVVQPVSNFFSTLAELPSLAARNAELERQVEDLRTAQQLNQELARR
                     IEDLEGLIGLQEILPRTIPAQVIASGVSNSEWTVTIGAGSDHGIALNMPVVTGASDGP
                     RLVGRVIQVTPISSMVQLLIDRDFAVPATLSTSHEAGIVEGRGEDELRMRLVGPGVEV
                     SETEPESVFTLGYRVEGEAGLYPPGILIGTVSRAFSEAGSVESFVTIRPAVDFSTLQN
                     VLVIARRRAAAEGAT"
     gene            8051..8557
                     /gene="mreD"
                     /locus_tag="VF195_02050"
     CDS             8051..8557
                     /gene="mreD"
                     /locus_tag="VF195_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEX6579639.1"
                     /translation="MRRTVILVAVIVTALLLQTTVFPELTFLGVKPELLYLVTVVFAA
                     LRGPAEGAVVGFVSGLSQDFLLNTPKGITALTLTMLGYTIGLARQYITSPSPLLPAFL
                     VAVGTAGGVAFQRVVAFLLGEEEGPFGYVAKVVVLTALYGAVLTPIFYPVLRRAAAAT
                     HAGRVVRF"
     gene            8558..10432
                     /gene="mrdA"
                     /locus_tag="VF195_02055"
     CDS             8558..10432
                     /gene="mrdA"
                     /locus_tag="VF195_02055"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6579640.1"
                     /translation="MERAGSRLRILAFVVAFMFVALSTRLWFLQVLAGPQHERDARDN
                     SLRTVTTDALRGDILDRDGRRLVHNRQSLEVRINRDELGDEAEATLAHLSEILGVSAQ
                     DLGEELDSKLYYSYQPVPVAEFVPEEVYFKVREEPERFRGVEVVQQSVRTYPQGPLGA
                     HLVGWVGQINAEEIRERRFEGYGPSDLVGKAGLEATYERWLRGTPGEERFLVNSDGEV
                     LREFDPEPARPGHDLRLSLDLDIQRIAEAELLTGIENARTVFDESSGTNLVANAGAVV
                     VLDPRTGGIVAMASWPSFRPSWFVRGLTQNQRSLLFESEQAPMLNRATQITYAPGSTF
                     KPFVALAAVKEKIASFGSYYDCPAEYVHPGDESGTVFENWSGLSGGTLSIAGGLKVSC
                     DSQYYAWGSEFWRRDTRTNEQELQRHVRQWGFGRETEVDLPAEAVGTVPDHEYVAEHD
                     EVYPDGWIPGIDILLAIGSGEMKATPLQVAQAYAALASGRLCRPHLVARIEAGDGAIA
                     KRIGGRCRPVDYTAGELDYIRDALRGVTTTGGTAGSVFARCPLDVSGKTGTAERPPFQ
                     DTSWFVGMVPSEDPEYVVVATVEQGGFGAETAAPIVRNIMSRIYRTPCEGPDLGEAED
                     "
     gene            10432..11574
                     /gene="rodA"
                     /locus_tag="VF195_02060"
     CDS             10432..11574
                     /gene="rodA"
                     /locus_tag="VF195_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX6579641.1"
                     /translation="MDLVAGRVYADERRPIRHIDGLLLLVTAILIVLGFFLLYSATNQ
                     TLRQDRLDPFLRVNKQILTAALGVILILAMATFDYRFLKVYAGFVYAGVVVALLLVRI
                     PFLGSTAAGAQRWFEIGGFQVTPSEFAKIGLVIMLAASLSELKHGFPSAQDMIRMCGV
                     ALVPLVLVFIQPDIGTSIVMVAIAAGMFLVAGVRGRHLAALVVICLVLIAAAFQLGVV
                     KDYQLDRLRAFIDRENTPADVRYNLDQAEIAVGSGGLLGQGYLHGRQTTLDYVPEQHT
                     DFIFTVAGEEFGFLGSTFVLTLYALLLWRSIRIAFLSKDPFGTFLASGVAIMFAIQMF
                     VNIGMVIGIMPITGIPLPFLSYGGSSMLASCLGVGILLNVHMRRFK"
     gene            complement(11648..12826)
                     /locus_tag="VF195_02065"
     CDS             complement(11648..12826)
                     /locus_tag="VF195_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6579642.1"
                     /translation="MALHTLPVTQSVRPQIRVAVVDDNPGFRETLASLLGTEELIVVG
                     AAGSGAEAVGLVRSTEPDVVLMYVRMPKMNGIEATRLLKSTFPDVGIVALTGVEDQDA
                     VREMLVAGASCYVLKDSDSEEIFHAIRQAAAGGGVISSVVTPRVIGELTEALERERRR
                     SRQLELAQEALLERAARRHELVSRLGHELRTPVTVILGMAQTLSKGTGTHDQREEMLA
                     ALVMRAQGLARLVSRLEGVVEAGLTEYTDVIDVAEEIARMSPRVHVEAPGRPMLATLN
                     RVAALRILEELVENGLEFSDEREPVTVRIGEADAGIEVRVVDRGTGVAEDAFERIFEP
                     LEQAEELHTRTHQGVGLGLTVARMSARAMDGDVTLEETGPHGSVFLWRVSMGPFSDVP
                     "
BASE COUNT         2011 a         4512 c         4630 g         2105 t
ORIGIN      
        1 gctcgcgacg aggtcgacgg cctggccgac cgtcttcacg gactccatct cgtcctcggg
       61 gatctcgatg ctccattcct cttcgagggc gagcaccgcc tcgaccaggt cgagggagtc
      121 ggcgttcagg tcctcggcga atcgggcgtc ggggacgacc tgttgctcgt cgacggccag
      181 ctgctcggcc agcaccttct tcaccctggt ctcgatctcc gcacgatcca tgcttcctcg
      241 ctttctctct tgacttctgt ctctggggct tctgtctctg gcttctcttc ggctgctcgt
      301 cctcgtcgtc ttctcgttcc ctcggtcgtt cgggcgagcc tatccgcgga ccggagcgcc
      361 gactacgcgg tgagcccccc atcgaccggg atcaccgtgc cgttgacgta ggacgcttcc
      421 tccgacatca ggaatcgcac gacgcctgag acctcctcga gcgtggccgc ccggcgcacc
      481 ggggtctggt cgagatagaa cgcgcgagcg tcgtcggtga ggtgcgcggt catctcggtc
      541 tggagcaacc cgggacagag cgcgttcacc gtgatgccct tgcctccgac ctcccgggcg
      601 agggacttcg tgagcccgag gaggccggcc ttcgtcgccg cgtacacggt ctgccccgcg
      661 ttgccgtgga tcgccaccgc gctcgacatg ttcacgatcc gcccccactt ctcgcgcagc
      721 atgctccgga gggcggccct cgagcacagg aacgatccgc ggagattcgt ggtcatcgtc
      781 cgttcgtact ccaccatcga gtacttgagg agcagcccgt cccgcgagta ccccgagttg
      841 ttcaccagac cggtgaccgg gccgacctcg tcgttgaccc gtcggaacgc ctcctggacg
      901 ctcgactcat ccgtcacatc gaggtacacc gtcatgccgg gtgttcccgc cgattccagg
      961 gcctcgagcg tctccttggc gtccgcctcg cgggtccgat aaccgacggc gaccgtccac
     1021 cccgcggcgg cgagcgcctc ggagcacgcg cgaccgatcc cgcgggaggc gcccgagatc
     1081 atcgcgacct tgggggtcac tcgggcccct gcatcgtcca ccggaccagg gtggcgcccc
     1141 aggtgagacc ggcgccgaac gaggtgaaca ggacgaggtc gccctccttg atccggcccg
     1201 cgttccatgc ccgcgcgagc gcgatcggga tcgaggcggc gctggtgttg ccgacctcgg
     1261 ccacgtccag gaccgagcgt tccgggggga cgcccagtcg ttccgcgacg gcgaccatga
     1321 tgcgggagtt cgcctggtgg gggatcagga cgtcgacgtc ggcgggcgtg tgctccgact
     1381 tctcgaggag ctcccggcag gcggcggcca tctcggtcac cgcgcgcttg aacacctctc
     1441 gccccttcgg catccggatc gagtgatcgc gggcgtcgac ggtggcatgg gacgcaggca
     1501 tgcgcgtgcc gccggccggc atctggagga tgtcggctgc cgtcccgtcg gcgccaagga
     1561 cggttccctc gatccccggt cggtcggatg cctggacgat cacggcgccc gcaccgtcgc
     1621 cgaagaggat cgacgtcgtc cggtccgtga agtcgaggat ccggctgaag atctccgcgc
     1681 cggccagcag gaccgtgtcg gccatccccg cctgcacgaa cgcggtggcg ctcgcgaggc
     1741 cgtagctgaa gccggcgcat gccgcgttca cgtcgaacgc cggcacccgg acgcctagct
     1801 tctcctgggc ccagacggcg gtcgcgggga acggcgtgtc gcccgtgacg ctcgcgacga
     1861 cgatcatgtc gagctgatcc ggcgtgatcg ccgccgtggt catcgctgcc cgcgccgcgt
     1921 cgacgacgag gtccgacgtg tcgacgccct cctcgacgaa gtggcgggcc tcgatcccgg
     1981 tccgctcccg gatccactcg tcgttggtgt cgatcttgtc ctcgaaccac gtgttgggaa
     2041 cgagacgagg agggagcgtg gacccgacgc cggtgatggt ggcgctcctg gtcacggggc
     2101 gagtgtagtc ggtcggcggc ccacagtcgg tcgcctaggc cgtgcccgaa gcggtggccc
     2161 gtgcatcatc gggagagccg accgcccggg cgaccgcgtc gggcaggatc cgcttcatca
     2221 gcttggtgag cacgtcacct ggacccgctt cgaggaacgc gccggccccc gcctcggcga
     2281 gggcgaggat cgaggacgcc cagcggaccg gggacacgac gtgtcgcgtc aggagggaac
     2341 ggagctcgtc cgggtcggac acgagggcgc ccgccacgtt ctccgccacc gggcaccggg
     2401 gcgcggagaa cccgatcgcg tcgatgcgct cctcgatcgc cgcgcgcgcc ggctccatca
     2461 gcggcgaatg gaaggcacct gcgaccggaa gtcgaaccgc gcggatcctt cgctccttgg
     2521 cgagcgtctc ggctcgctcg atcgccgcga ccgagccgga gatcaccacc tgcacggggg
     2581 agttctcatt cgccaccacg acgtcgtcgc cgccgcggac ctcgtcgcag agggacgcgg
     2641 catcctcggc gccgacgccg agcagggccg tcatcgctcc gggccgctcc tcgccggcgc
     2701 gctgcatcgc ggcgcctcgg atcaccacca gatcgagcgc gtcggccaag gagaggacgt
     2761 cggccgcgac gagcgccgcg aactggccga gggaatgacc ggccacgccg accggggcga
     2821 ggccttccgc ttcgaggacg tggaacgccg caacgtcgca ggcgagcagc gcgggctgca
     2881 cgaactccgt ggtggcgagg gcatcgtcgt cgtggcaacc gaccacgatg tcgcgaccca
     2941 tcgcgtcgga cgcctcctgc aggatcgatc tcccggaggg atggccgatc cacggatcgg
     3001 ccatccccgg gtactgggag ccctgtccgg ggaacaggac cgcgaccgcc ggtaggtgcg
     3061 ccacggccgg cgagcctatc cgcagcgagc ctcggccggt cgcgagcctc ggccgccgcg
     3121 agcctcggcc tttcgcgtcc tccggcggtt tgcgccgtca tggcctcggg cgtacgctcc
     3181 tgcgacgacc gatcgacccg tgcgaacggc ctgccgcggc tccacccccg ccgcgcgggc
     3241 cgagtgcggg tccgcgaacg gaagggggaa cgatggcatc gaccgtcgag gagctgcgcg
     3301 gccgaaccac cggccaggtg atcacccagg acgaccctgg ctacgacgag gcacgcgccg
     3361 tgtacaacgc gatgatcgac aagcgtccgt ccgtggtggt ccgggcgacg gaggccgccg
     3421 acgtggtcgc cgccgtgaac cacgcgaggg agaacggtct cgacgtcgcc gtccgcggcg
     3481 gaggccacag cgttcccggt ttcggcaccg tggatgacgg cgtcgtgatc gacctctccg
     3541 ggatgcgagc ggtcacggtg gatccgagtt ccaagacggc ctcggcacaa ggcggcgcta
     3601 cgtggggtga cttcaacgac gccacgaacg cccacggact cgccacgacc gggggcatca
     3661 tctccacgac cggcgtcgcg ggtctctccc tgggcggtgg tatcggctat ctcgcgcgcg
     3721 gcgcgggact gtcgtgcgac aacctgctct ctgccgtcgt ggtgacggcc gacgggcaga
     3781 cggtgacggc gaacgcgacc gaacacgagg atctgtactg ggcgttgcgc ggcggcggcg
     3841 ggaacttcgg gattgcgacg tcgctcgagt tccggctcca tcccgtcgcc gagatctacg
     3901 gcggtccgat gttcttcgag gtcgctgacg ccggagatgt cctccgttgg taccgtgagt
     3961 tcatcgccga tgcgcccgag gagttcgggg gcttcccggc gtggcagatc gcgccaccgt
     4021 tgccgttcgt tcccgaggag cgacacggcg atccgttcct cgcgttcgtg gcgtgttggg
     4081 ccggacccgt cgaacagggt gagcagatgt tgaagccatt gcacgacgtc gcgccagtgg
     4141 tggccgagca cgtcggcccg atgccgtatc ccgcgctcaa cagcgcgttc gatggcctcg
     4201 tgccaccggg cctgcagcac tactggaagg ccaacttcgt gaaggagctc acggacgacg
     4261 cgatcgcggc gcacctggac cacggcccga acgtgccggt ggtgaactcg acggtccaca
     4321 tctatccgat caacggagca tgccatcgcg tggcgtcgga cgagaccgcc ttcgcctacc
     4381 gcgacgcgac gttcgcgacc gtgatcgcgg gcatgtggcc ggatccggcg gacaacgaga
     4441 agaacacggc gtgggtccgc gactactacg gcgccacggc tccgctctcg gaagagggcg
     4501 ggtacatcaa cttcatggcg gaggacgacc aggaccggat ccgggcgaac taccgaggca
     4561 actacgaccg gctcgtcgag gtcaagcgcg cctacgaccc gggaaacctg ttccgccaga
     4621 accagaacat cgtcccgtga ggggttgcct atgcgcgccg tcgggctcgg ccgagaagag
     4681 ctcccaccgg cccgggatcc ccttgagatc gtgcgtcccg cgatcctcga accggatccc
     4741 cgcgccggat gagagctcac gcaccgtgcg cgagacgagg atctcgtgcg gcgatgccaa
     4801 ggccgcaacg cgggccgccg tgtggaccgc gacgccctgg atgccgtcct cagacacctc
     4861 gcactcgccg gtgtggatcc ccgtgcgaag ctccagcccg agggtcgacg tcgcctcgat
     4921 gatcgaccga gcgcagcgga tcccggtggc ggggacgtcg aatgtcacga ggaaaccgtc
     4981 gccggcggtc tcgacctcgt gtccgtccga tgccgcgatc tggctcctca cgacccgatg
     5041 gtgctcctgc aggagggttc gccaggcacg gtcaccgagt cgcacggccg tctgcgtcga
     5101 cccgacgatg tcggtgaaca ggatcgtcgc gagctttcgg tcgaggcgac gggcgggagc
     5161 gaggccgccg atgaaggccc gcacctcgtt gacgatcgtg gtttggtctc ctacccacat
     5221 caggtgatcg acccccggaa gctcgacgaa ggtcgatccg gcgatcccgt cggcgagtgc
     5281 tcggccatgt gcgaagggga cgatcaagtc accccggcgg tgcaacacga gcgtcggcac
     5341 acggatcgcg gggagcgacg ccgtccagtc cacctggaat ccgagctcca tcatgccgcg
     5401 cgcagacgag gggctcatgc tggaacgaag cagccgcagg taggagtcgg cgaggagctc
     5461 gtcgccgagc gcgctgggtg cccacggatc gagcccgacg ctcgcgtgcc cccactcctt
     5521 ctcaacacgg tcgagtgcga cctccggcga gtccgccagt cgggccagca accctgtcgt
     5581 cggttcgagt ctcgcgaacg tgccgtacag gatcagggcc cgggtccgtt ccgggtagga
     5641 ggccgcgaac aagccggaca tcgctccgcc accgagcgtg ccgaagagtg ccgcatgctc
     5701 ggccccgccg catccatcac cgctcggaga tcgctcatcc gctcctcgag cgtagggagc
     5761 gaatccggcg agaagcgatc tgacagaccg gtaccccgct tgtcgaagag gatcagccgg
     5821 gagaaggaag ccaggtcgtt cagcaaccga gcgaggggag ggatgtccca ggccagttcg
     5881 agatgcgttg cccagccggg cgatagcacc agatccacgg gtccgtcgcc gaccacctga
     5941 tacgcgatgt tgaacgcccc ggccttggca tatcggacct tccgttccac ggccggagtc
     6001 taggcatggc tccagcggat atgcctcctg agcggatgcg agttgccacg cggaggggaa
     6061 ccctgttcaa tcagggcgag cccatggccg agagggttca gagatcttct tcccccacgc
     6121 cccgagcaca ggggtcggag ggggtattgg ggttccttgg tcgcgacatg gctgtcgacc
     6181 tcgggacggc gaacacgctc gtgtacgtgc gtgggcgcgg agtcgtcctg aacgagccgt
     6241 ccgtcgtcgc gatcaacacc cttactggag ccatccttgc cgtgggtgcc gaggccaagc
     6301 ggatgatcgg ccgcactccc tcgcacatcc aggccgttcg cccgctgaag gacggcgtga
     6361 tcgccgactt cgacgtgacc gagaagatgc tccgctactt catccagcgg gtgcacaagc
     6421 gccgactcct cgcgaagcca cgtgtggtcg tgtgcgtgcc atccggcatc accggggtcg
     6481 agcaacgcgc cgtcgaggaa gcgacgatcg ccgccggcgc gcgccgcgcg tacatcatcg
     6541 aggagccgat ggcggccgcc atcggtgcag gtctcccgat ccacgaaccc accgggaaca
     6601 tggtcgtcga catcgggggt ggcaccaccg aggtcgcggt gatctcactc ggcggcgtgg
     6661 tcacctccac ctcgatccgt atcggcggcg acgagctcga cgagtcgatc atccagcacg
     6721 tgaagaagga gtactcgctc ctcctcgggg aacggacgtc cgaggcgatc aagctcgcga
     6781 tcgcgtcggt ctatccgatg ccggaggagc tcgtcgccga gatcaagggc cgcgacctca
     6841 tctccggcct cccgaagacc atcacgatcg ccgcggaaga gatccgccgg gcgatcgagg
     6901 agccggtgaa cgccatcatc gacgccatca agaccaccct cgaccggacc ccccctgagc
     6961 tcgccgcgga catcatggac cgcggcatgg tgctgacggg cggaggcgcc cttctccgcg
     7021 gtctggacaa gcgcctccgt cacgagacgg ggatgcccgt gcacatcgcc gacaccccgc
     7081 tccaggcggt cgtgctcggc tcggggaagt gcctcgagga gttcgaggtc ctgcagcggg
     7141 tcctggtgac gtcgaccagc cggcggtagc ccgccgtcgt ggcgatccgg caacgaaccc
     7201 gctccgccag gctcctggtc gtcaccttgg tcgccgcctc cctcgcgatc atcaccgtgg
     7261 actaccgcgg cggcgacgac ggaccgctcg actcggtggg cgaggcgatc agcagcgccc
     7321 tggccccgat gcagcgggcg gtctccggcg tggtccagcc ggtcagcaac ttcttcagca
     7381 ctctcgcgga gctcccgtcc ctcgcggcgc ggaacgccga gctcgagcgg caggtcgagg
     7441 atctgcggac cgcgcagcag ctcaaccagg agctcgcgcg ccggatcgag gatctcgagg
     7501 gactcatcgg actgcaggag atcctccctc ggacgatccc ggcccaggtg atcgcgagcg
     7561 gcgtctccaa ctccgagtgg accgtgacga tcggcgccgg gtccgaccac gggatcgcgc
     7621 tcaacatgcc ggtcgtgacg ggagcctccg atgggccgag gctcgtgggc cgggtgatcc
     7681 aggtcacgcc gatctcctcc atggtgcagc tcctgatcga tcgcgacttc gcggtgccgg
     7741 cgaccctctc cacgtcgcac gaggccggga tcgtcgaggg acgcggtgag gacgagcttc
     7801 ggatgcggct ggtggggccc ggcgtcgagg tgagcgaaac cgagccggag tccgtgttca
     7861 ccctcggcta ccgggtcgag ggtgaggcgg ggctctatcc gccgggcatc ctgatcggga
     7921 ccgtgtcccg cgcgttcagc gaggccggct cggtcgagtc cttcgtcacg atccggccgg
     7981 cggtcgactt ctcgacgctg cagaacgtcc tcgtgatcgc gcggcgtcgt gccgcggccg
     8041 agggcgcgac gtgagacgga ccgtgatcct cgtggccgtg atcgtcacgg ccctcttgct
     8101 gcagacgacg gtcttccccg agctcacgtt cctcggggtg aagccggagc tgctctacct
     8161 ggtgaccgtc gtgttcgcgg cgctccgggg accggccgag ggcgcggtcg tcgggttcgt
     8221 cagcggcctg tcgcaggact tcctgctcaa cacgccgaag gggatcacgg ccctcaccct
     8281 cacgatgctc gggtacacga tcggcctggc ccggcaatac atcacgtcgc cgtcgcccct
     8341 gctcccggcc ttcctcgttg ccgtcgggac cgcgggtggc gtggccttcc aacgggtcgt
     8401 ggccttcctc ctcggcgagg aggaagggcc cttcggctac gtcgcgaagg tcgtggtcct
     8461 cacggctctc tacggcgcgg tgctcacgcc gatcttctat cccgtgctgc ggcgcgcggc
     8521 cgccgcgacg cacgccggaa gggtggtgcg cttctagatg gagcgtgcgg gctcccggct
     8581 gaggatcctc gcgttcgtcg tggcgttcat gttcgtggcc ctctcgacgc ggctgtggtt
     8641 cctgcaggtc ttggcgggac cccagcacga acgggacgca cgcgacaact cgttgcggac
     8701 ggtgacgacc gatgccctgc gcggtgacat cctcgatcgc gacggtcgac ggctcgtcca
     8761 caaccggcag agcctcgagg tccgcatcaa ccgtgacgag ctgggggacg aggcggaggc
     8821 gacgctcgcg cacctctcgg agatcctcgg cgtctccgcg caggatctcg gcgaggagct
     8881 cgattcgaag ctgtactaca gctaccagcc cgtcccggtc gcggagttcg tgcccgagga
     8941 ggtctacttc aaggtgcgcg aggagccgga gcggttccga ggcgtggagg tcgtccagca
     9001 gagcgtccgc acctacccgc aggggccgct cggcgcccac ctcgtgggtt gggtcgggca
     9061 gatcaacgct gaggagatcc gcgagcggcg gttcgagggg tacgggccgt cggatctcgt
     9121 ggggaaggcc gggctggagg cgacgtacga acgctggctg cgcgggacgc cgggggagga
     9181 gcggttcctc gtgaactccg acggcgaggt cctgcgggag ttcgatccgg agccggcccg
     9241 acccggtcac gatctgcggc tgtcgctcga tctcgacatc cagaggatcg ccgaggcgga
     9301 gctcctcacg ggcatcgaga acgcacggac ggtcttcgac gagagctcgg ggacgaacct
     9361 cgtcgcgaac gccggcgccg tcgtcgtcct cgatccccga acgggtggca tcgtcgcgat
     9421 ggcgtcgtgg ccgtcgttcc ggccgtcgtg gttcgtccgg gggctcacgc agaaccagcg
     9481 ttccctgttg ttcgagagcg agcaggcgcc gatgctgaac cgcgccaccc agatcacgta
     9541 cgccccgggc tcgacgttca agccgttcgt cgcccttgcc gcggtgaagg agaagatcgc
     9601 ctcgttcggc agctactacg actgcccggc cgagtacgtg cacccgggcg acgagtccgg
     9661 cacggtcttc gagaactgga gcgggctgag cggcggcacg ctgtcgatcg cgggcgggct
     9721 caaggtctcg tgcgactcgc agtactacgc gtgggggagc gagttctgga ggagggacac
     9781 gcgtacgaac gagcaggagc ttcagcgtca cgtgcggcag tggggcttcg gtcgcgagac
     9841 cgaggtcgac ctcccggcag aggcggtcgg gaccgttccg gaccacgagt acgtcgccga
     9901 gcatgacgag gtgtatccgg acggctggat cccggggatc gacatcctgc tcgcgatcgg
     9961 ctcgggcgag atgaaggcga cgccgctgca ggtcgcgcag gcgtacgcgg cgctcgcgag
    10021 cggacggctc tgccgacccc acctcgtggc gaggatcgag gcgggggacg gcgcgatcgc
    10081 gaagaggatc ggcggcaggt gtcggccggt cgactacacc gcaggcgagc tcgactacat
    10141 ccgcgatgcc ctccggggcg tcacgacgac gggcgggact gcgggatcgg tgttcgcgcg
    10201 gtgtccgctc gacgtctccg gaaagacggg gaccgcggag cgcccgccgt tccaggacac
    10261 gtcgtggttc gtcgggatgg tcccctcgga ggatccggag tacgtggtcg ttgccaccgt
    10321 ggaacagggg gggttcgggg cggagaccgc cgctccgatc gtccggaaca tcatgagcag
    10381 gatctatcgg acgccgtgcg agggcccaga cctcggggag gcggaggact gatggatctc
    10441 gtcgcgggac gtgtctacgc ggacgagcgc cggccgatcc gtcacatcga cgggctcctc
    10501 ctgctcgtga cggcgatcct catcgtcctc ggcttcttcc tgctctactc ggcgaccaac
    10561 cagaccctcc gccaggatcg gctggatccg ttcctgcgcg tcaacaagca gatcctgacc
    10621 gcggccctcg gggtgatcct catcctcgcg atggccacgt tcgactaccg cttcctcaag
    10681 gtctacgccg ggttcgtcta cgccggcgtg gtcgtggccc tgctgctggt ccggatcccc
    10741 ttcctcggct cgacggccgc gggcgcgcag cgctggttcg agatcggcgg cttccaggtg
    10801 acgccgtcgg agttcgccaa gatcggcttg gtcatcatgc tcgccgcgag cctgtccgag
    10861 ctgaagcacg gcttcccgag cgcccaggac atgatccgga tgtgcggcgt ggcgttggtc
    10921 ccgttggtgc tcgtcttcat ccagcccgac atcgggacgt cgatcgtgat ggtggcgatc
    10981 gcggccggga tgttcctggt ggccggggtg cggggccggc acctggcggc gctcgtcgtg
    11041 atctgcctcg tgctcatcgc ggccgcgttc cagctcgggg tcgtgaagga ctaccagctc
    11101 gatcgtctgc gtgcgttcat cgaccgggag aacaccccgg ccgacgtccg ctacaacctg
    11161 gaccaagccg agatcgcagt ggggagcggc gggctcctcg ggcaggggta cctccacgga
    11221 cgccagacga cgctggacta cgttcccgag cagcacacgg acttcatctt cacggtggct
    11281 ggggaggagt tcgggttcct gggctccacg ttcgtgctga ccctctacgc tctgttgctg
    11341 tggcggtcga tccggatcgc gttcctgtcc aaggatccct tcgggacgtt cctcgcctcc
    11401 ggcgtggcga tcatgttcgc gatccagatg ttcgtgaaca tcggcatggt gatcggcatc
    11461 atgccgatca ccgggatccc gcttccgttc ctctcgtacg gagggagctc catgctcgcg
    11521 agctgtctgg gagtggggat cctgctgaac gtgcacatgc gccggttcaa gtagcgcctc
    11581 gggaccttct cagactcaag cagcggcgcc acttcaagga agcggcgccg ttcaaggaac
    11641 ggcgcgctca ggggacgtcg gagaacggac ccatggagac gcgccacagg aacaccgagc
    11701 cgtgcggccc cgtctcctcg agcgtcacgt ccccgtccat cgcccgagcg gacatgcggg
    11761 cgacggtcag gccgagcccg acgccctggt gggtccgggt gtggagctcc tcggcctgct
    11821 cgagcggttc gaagatccgc tcgaacgcgt cttcggcgac gccggtgccg cgatccacta
    11881 cgcggacctc gatgccggca tcggcctccc cgatccgcac ggtgaccggt tcgcgctcgt
    11941 cggagaactc gagcccgttc tcgacgagct cctcgaggat gcggagggcg gcgacgcggt
    12001 tgagggtggc gagcatcggc cggccgggcg cttcgacgtg gacgcgtggg gacatccggg
    12061 cgatctcctc ggcgacgtcg atcacgtcgg tgtattcggt gagccccgcc tcgaccaccc
    12121 cctccagccg agagacgagt cgggcgagtc cctgggcgcg catcacgagt gcggcgagca
    12181 tctcttctcg ttggtcgtgg gtacccgttc ccttcgacag ggtctgggcc atcccgagga
    12241 tcacggtcac gggggtgcgg agctcgtggc cgaggcggct cacgagctcg tgccgcctgg
    12301 cggcgcgttc cagcaacgcc tcttgggcga gctcgagctg acgggagcgg cgacgctcgc
    12361 gttcgagcgc ctcggtgagc tcgccgatca cgcggggggt cacgaccgac gagatcacgc
    12421 cgccaccggc ggctgcctgc ctgatcgcgt ggaagatctc ctcgctgtcg gagtccttga
    12481 ggacgtagca ggaggcgccc gcgacgagca tctcccgcac ggcgtcctga tcctcgacac
    12541 cggtgagcgc cacgatgccg acatcgggga aggtcgattt cagcaggcgt gtggcctcga
    12601 tcccgttcat cttcggcatc cggacgtaca tcaggaccac gtccggctcg gtggagcgca
    12661 cgaggcccac ggcttcggcg ccactccccg cggcacccac cacgatgagc tcctcggtgc
    12721 cgagcagcga cgcgagggtc tcgcggaagc ccggattgtc atcgacgacg gccaccctga
    12781 tctgggggcg cacggattgg gtcacgggga gggtgtgcag cgccatcacc tggaagtgtc
    12841 ggcatccggg ggggcacagc ggtatcccgc acctgcgggg ttccctcggg gggactccgt
    12901 cggtggtttc ttagctcgga atgactagct cacaacgagg acggctggtt ccggaggggg
    12961 aggccatcgg gtcgggcgcc cgctcgcgag ggggttcgcg gagccggctc cgtccgatcg
    13021 gccaagggac tgttatcctc gatgcacaga cgtgccccaa cagggccggc agccagaagc
    13081 gccgctcgtg gtggggaaag aagcgagtga ggtggcgcgc gacgccgccg gcggccccga
    13141 agtggggatc accaggcagg cggcgcggcc ccggacagga ccgacagttg tacgagcagg
    13201 tcttcatccc ggtgcgcggc gatcgccgct gcgccggcga gggaacgctc atcgcgca
//
LOCUS       DASUYD010000039        39151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_682702, whole genome shotgun sequence.
ACCESSION   DASUYD010000039 DASUYD010000000
VERSION     DASUYD010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 39151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39151
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682702"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="VF195_02070"
     CDS             <1..753
                     /locus_tag="VF195_02070"
                     /inference="COORDINATES: protein motif:HMM:NF039288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX6579643.1"
                     /translation="GASTIEAQDKAGTTVAEMVRLALEGQFVPYAVNVSAGAEVSEAV
                     RPFVGLAERLGSLVAGLADAGVRSIVASYLGRVAEHDTRVLTLAILKGILGRSVNEPV
                     SFVNAPLLARDRGLTVSEMRSTGSQDYVSQISLRAQTESGSEIDVGGTLLGKKNAERV
                     IQVWDFEVEMEPAPYMLFFVYEDRPGIIGKVGTILGEHDINIATMEVGREAAGGDALM
                     ALTVDSPVPPDVLRVVEDAIDAHTIRAVTLDT"
     gene            932..2218
                     /locus_tag="VF195_02075"
     CDS             932..2218
                     /locus_tag="VF195_02075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579644.1"
                     /translation="MTRSPKSNVRRLALGRLISVMGGAAAYTALNYTVWERTHSPFMQ
                     AVSLLLTFGVAGLIGPFTGALGDRYNRRTVMIWSEAISAAFFAAMVFAKDPGLLIALA
                     FGSAIAELPFFSASRAAIPNLVESEKDLSWANSLVSMGVHAGIAVGPVIGGGLVAILA
                     PGDTPLPDRLHMAGAVVFGLNAISFLISLAVTLTVRGRFEEERSPEAEPEHEGLFAGA
                     AYLWRERVLRRMAFAWLVFVLGMGMGMVADAPLAESFGTGGWGFGLLIACWGTGSVLG
                     TVFGRWMNARTEPLWMVFGAAGVAVSAFGVGLFGLFPLVLVSLLVMGTSDGISIVAEN
                     GIMQRRTPDVVRSRAMAALEAVLSLGLAIAYLAAGPVLRLVEAQTVYVIAGVFAVGAT
                     IVLLPLIRLKADASDGRPTGPGVPVQQPTTGDRATV"
     gene            2231..3517
                     /locus_tag="VF195_02080"
     CDS             2231..3517
                     /locus_tag="VF195_02080"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579645.1"
                     /translation="MTEGTGRAAVRRVAAARGISLTGSGAAFAALAYIVYSLTGGSAV
                     WLSVTLLLTMGLQGVVQPFASWLGDRFDRRRVLVISDLAAAAGFVALAFARTPGQLVM
                     VAAITAVLESPVWAVAGASIPNLIEEEHLPWANGQVAIGRHLGSFIGPLLGTQLIVWL
                     AGEAPSVDRLLGAGAFVFGINAATFLASAWLIGSTAGRFNDERREESEHAGIRAGFRY
                     AMSDRVLRSILIGWSVLLLGVGLILVAELPYALGFGEGAFGYGLISALWGGGAALGAL
                     LAARWLTADREPRTLLVSVLLGGLVMVAIGWSPVWILALGLMVTEGIFEGFASVSEQG
                     LLQRRTPDDVRSRVAGAVEAATLIALAISLTAGGPIVDWLGPRAAYYLGGLMTVGAGL
                     VMAGPLLYPGLPRHRADEAFVFEPAERVVSDRSART"
     gene            complement(3514..5838)
                     /locus_tag="VF195_02085"
     CDS             complement(3514..5838)
                     /locus_tag="VF195_02085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6579646.1"
                     /translation="MTSPTPLNARDFPIQDLSFHHVLGSMPGVAYVETEGPTGTMAYM
                     SPRIEALTGYPPERFTESREFWYSLVHPDDRERVELSDGVADAEKDPYLEEYRLVVVD
                     GRVIWVRDEAVHVDASEGRSAHWIGLIVDITKEKEAEARAAEVEHRYRTLVEQLPIVT
                     YIDEPDESLANLFVSPQVNDLLGRPSGSTGEEWAERVHPDDRDRARHETVEGVRSGEP
                     FTLEYRMVRDDGRVIWVRDSATTVRNDAGEPGYVLGFFFDITKRKEAELQLEELERRY
                     RNLVEQLPAVTYMDTADDDLTSIYVSPQVENVLGLSADAFISDDAWAGHLHPEDRDEA
                     VRGIREAIRRAEPFTLEYRMIRPDGSIVWIRDQGTVVKDEDDHAAYVQGLYVDISEQK
                     RLESELRSEAVKFKSLAEQIPAVVYIEGEGGSGDSPIYISPKYQELFGYTPEERQARP
                     NLWRELLHPEDRDRVLLAEEQANDEHPFSEDYRMVARDGRLVWVHDETVPIRDESGEP
                     LFYQGVMYDVTEQKRAERELAQALDMERKAVERLREADDMKNTFLTAVSHDLRTPLSA
                     ILGSAITLESADELGISQEDRRQLVRSLARKSRRLTAMVNDLLDMDRLTRGLVQPRRE
                     LMDLGALLGRTVAESDVLEERTVHVQTEPIDAWIDESMVVRIVENLLVNAIKHTPPTS
                     EIWVGARRVDNGVIVRVDDDGPGVAEDERDRLFEPFERGERSAPSPGLGVGLSLVARF
                     AEAHDGRAWVEDREGGGASFRVLFPDPATRLPSS"
     gene            complement(5952..7151)
                     /locus_tag="VF195_02090"
     CDS             complement(5952..7151)
                     /locus_tag="VF195_02090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579647.1"
                     /translation="MSAKRSENDAAADHVVELPYRSLVERVPAIVYVDPVGPEPTAPS
                     YVSPFIETMLGYPPEVATGDGGWWASALHPDDRERVVAEWSRSDASGEPYSGEYRLLS
                     ADGRTVWVRDEAVLLRDHEGTPVHWQGVMMDISEAKAATEDLTRALDLERLAADDIRR
                     ADEIKTTFLTAVSHDIRTPLSAILGNALTLEEGDRLGITEEERKQLIHSLAAKARRLN
                     TIVTDLLDTERLARGVVEPKLQEIAVGRLISLLVWDSEAMAGRQVHVDTRSVSAWVDP
                     QMISRIVENLLMNAARHTPSECQAWVKVLPEGDHVLIEVEDDGPGIPEEHRQSIFEPF
                     EHGPMTSVHNPGVGIGLSLVAKFAELHGGRAWVQEREGGGASFRALIPRGDPEVVPQS
                     ALPGERP"
     gene            7258..8421
                     /locus_tag="VF195_02095"
     CDS             7258..8421
                     /locus_tag="VF195_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6579648.1"
                     /translation="MPETFTFYIQPWYRKSPYFEATKRAGCASWGLYNHMLLPTLYDD
                     PVTEYWALLRGVTMWDVSVERCVEISGPDALPFTNLLTCRDLTSCEVGQCKYVLITDR
                     EGGIVNDPVLLRIADDRFWLALADSDALLYVKGVAALAGMDVRVIEADVSPMQVQGPR
                     SKEVVRDLVGDEVAALPYYRCAEAEIDGIPVVVSRTGWTGEVGYELYLRDAARGDELW
                     RLVEEAGAPYAIRAIAPSEARRIEAGIFNYGSDMRTEDTPFHITGLERLVEDQEADFI
                     GKDALRRIAEEGVDRKLVGIDIEGAPMTDEGALNDFWPVHESGGDRAWSRIGKVTAGA
                     WSPRLERNIGYAWVPATHSAVGTPLALEARDGKRDATVATLPFVDPTKEIPKS"
     gene            8418..9197
                     /locus_tag="VF195_02100"
     CDS             8418..9197
                     /locus_tag="VF195_02100"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6579649.1"
                     /translation="MSGGSMRFDRAVEYYDRTRGLSEDASREMTALLAGELHDRGRTL
                     EIGVGTGLVALPLAAAGVRLVGLDLSAPMLAKLVEKAGGRLPFPLVVGDATTLPFADH
                     RFGAAVVRHVLHLVPTWRQVVAELARVVAAGGLVLVSSGQVPEPWHQVTDRFMDRVGR
                     PSFADALDSWETGAIVQAFRAHAAEARQLPTITQQVAQTMGEFLDQMAAGMHSWTWEV
                     EEERRREAVAELRAWALERWGSLDPPGGRDVAIEWLAFDLP"
     gene            9249..10175
                     /locus_tag="VF195_02105"
     CDS             9249..10175
                     /locus_tag="VF195_02105"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6579650.1"
                     /translation="MPITQVEKIWMDGELVPWQDAQVHVLSHAVHYGSGVFEGIRAYE
                     TSRGAAVWHLDEHLKRLFRSAKLYHTDIPYSLEAITQATKDVVLANELNACYIRPLVL
                     RGYGEMGVNPLQAPVNVVIAVWPWGAYLGEEALEQGVRIKISSWRRNSQNSLPAAAKA
                     TGQYINSVLAKVESLKAGYDEAIMLNEAGFVTDGSGENVFVVRDGVLTTPPISAGCLD
                     GITRGSTITLARDLGYEVREENLVRTDLYNADECFFTGTAAELTPIREVDDRVVGEGH
                     RGPVTKELQGAFFAATKGENEKYVHWLTYVND"
     gene            10175..12718
                     /locus_tag="VF195_02110"
     CDS             10175..12718
                     /locus_tag="VF195_02110"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX6579651.1"
                     /translation="MGESEERYRLPRTVVPSRYDLTLEPDLDAGTFRGSVDVALRVLE
                     SVTEIVLNANELRLEGATLTAADGGHSVEVSKILTDDDAERATIDLAEAADPGAWTLR
                     IVFAGELNPRLTGFYRSTYADGDGRTQVVGTTHFEATDARRAFPCWDEPDLKAVFATT
                     LVVKEGLTAVSNGPEVEREALGDGRMRIRFADTMKMSTYLAAWVVGPLELTEPADARG
                     VPVRIVHVPGKGALTPFALDVSVHSLNWFGDYYGIPYPEAKLDNIGLPDFAQGAMENV
                     GCVTYREQVLLLDPARSTQEEQLGVAETVAHELAHMWFGDLVTMRWWNGIWLNEAFAT
                     FMSMLAVDDFRPDWEIWNTFTRDRSNAFEVDALESTRPIEYEVRSPDDASGMFDTLTY
                     VKGGAVLRMLEQWLGADRFRDGIRRYLGEHAYGNTETHDLWDALEEETGEPVRRTMDA
                     WIFQPGYPAISVSLDGDRVRFSQRRFLPSRDDDDTTWPVPLTVRQVSGEQDQVDRILI
                     EGDGAEIALLAPDAVVVANAGSASFVRVWYDDELRSRVVGRAMDVLSPIERYSLVDDA
                     WAAVVAGQASAGSYLDLVQGFADETDLRVWQSILGGLGWLDRFVDDEPRERLRAFVRE
                     LLRPALARLGWDASPDETDLTRALRGQLVQSLAILGADPETLARCRDLELEGQGDPQL
                     LQAAVEAVAAEGTEDDLDRYWERYRNATTPQEEERYLFATSRFPGDAEVERILGASMT
                     DDIRTQDAPYLLARSTTNRRQGPKVWRFIAEHWDAMQDRFASSNIIGLVSGIRYLTDP
                     ESVADVDAFFREHDIPQNHLMLRQGLERMRVNAELRRRVTPELFERFGG"
     gene            12849..13928
                     /locus_tag="VF195_02115"
     CDS             12849..13928
                     /locus_tag="VF195_02115"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX6579652.1"
                     /translation="MRNRAARAAAVAILTTILLTAAAGSALACGGLVTPNGAVSLLKT
                     TTLAAYHDGIEHYVTSFEFAGEGQEVGSIIPLPGVPTDVIKGGDWTLQRLILETQPQL
                     EALRANSFLALDSAAGAEVLMTKKIDALDITVLKGGAVEVGDWAREHGYFLPPDAPEV
                     LAFYAERSPIFLAARFNAERAANRGVQTGQGTPVHIVIPTPSPWVPLRILALGRKASE
                     IVQADVYLLTDRRPETLPQAERSKGFDQSQRGLIQEVSEPASTTLLADLRSDRGMKWL
                     PESDMWLTYIRINETAGNLRHDLAIDASGYGRPSPVAAGYPVPAGAAPWSVVWTVMAA
                     LAAVVVLMAITDPAARRTLSRVFRR"
     gene            13925..14341
                     /locus_tag="VF195_02120"
     CDS             13925..14341
                     /locus_tag="VF195_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6579653.1"
                     /translation="MKLRATAAAVALVLLVGAAAASRGAAARANDGAIRLTIHYSKFD
                     PADLAVEPGETVRFVIVNTDPIDHEFILGDEQLQLVHEEGTEAHHAPRPGEVSVPAGA
                     IGVTTFTFPRISLSLIFGCHLPGHYDFGMRGLVTIG"
     gene            14614..16083
                     /locus_tag="VF195_02125"
     CDS             14614..16083
                     /locus_tag="VF195_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010040759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX6579654.1"
                     /translation="MSGRVEVEGVEVSTDHWIGGERVPSRSTFTDISPIDEGPIAEVA
                     RGGVDEANAAVDAAKKGFETWGAMSLKERAEILHRVGELIEERAPDIARVETRDNGSL
                     LRSMTNSVIPRAGYNFRFFADHLDKLDELEDLDTRGHSNHVSWDPSGVAVVITPWNAP
                     LMLATWRIAPALAAGDAVIAKPPEWAPLTASLLADITRDAGMPDGAFNVVQGIGEEAG
                     AALVADPDVGRVAFTGSVATGRLVAASAGANLTPVSLELGGKAPFLIFADTDLEAAVT
                     QATNQFDNAGQVCLAGTRLLVEDEIFDEFLARFTEASAAIRQGDPREETSDIGPLITR
                     EHFERVDGFVQRAKAEGIEVAFGGGSNEELGGLHYRPTVFLEPPSGSEILQQEVFGPV
                     LTLQRFTGEEDGIAKANDTTYGLSAIVYSGDQDKADRVSSALVAGTVWVNCFYVRDLR
                     VPFGGSRNSGIGREGGFWSFDFYADVKNVVTAPWDLTKT"
     gene            16098..16997
                     /locus_tag="VF195_02130"
     CDS             16098..16997
                     /locus_tag="VF195_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009774278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catechol 1,2-dioxygenase"
                     /protein_id="HEX6579655.1"
                     /translation="MGEIVGAGIVSHVPTIMLPEETRLEINDGKEITLVPGLRRLRSE
                     VLDRVGADTFVVMDTHWEVTFEHIITAHEKREGIFTSHELPRGMRQIPYDMPGDPELA
                     FTVSQQAEGRDDTWVLASDDPYLPIFYGTVNIWSYLKEGPENWVSVGINQCGTTEDFL
                     LLGQLIGEAVLSLDRKVVLLASGGLSHRFLPFRELRGRESSDPMKNIITPEAREADLH
                     VLDLLQKGDHAAVIDWVPEYRKVGPEGKFGHYLIMAGALGGVACTAPGELFSEYEASV
                     GTGQVHVWFERPAPGWTGSKEAA"
     gene            17037..18524
                     /locus_tag="VF195_02135"
     CDS             17037..18524
                     /locus_tag="VF195_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6579656.1"
                     /translation="MATETDRIGGDPGYERLGRRRLKLVDVVAQSVGLIGPVFSAAFL
                     IPLIAGFNFSGEGAGIATPFAVILASIGVLGLGWIVAQYAKRVHAAGSLYDYVTMGLG
                     SRIGGWAGWVYYGGTLLLSSAIAVLVGWFLRDNVLPAFEIDPIMPTWAWSLIYVGIVF
                     LILAAGVQISTRVQLLLALVSVAVVLAFFINVIVNAPENSVKAFNPAEAPSYTGILFG
                     VLYGVLIFVGFETAANLAEEAEQPKRAIPKAVMLSVGIVAVFYVIAAYAQVAGFGFDV
                     SVLTSPEVAAAPLFALGSPDAVGGMGGSSDTMLKILLVVVLLDVMAVGLGAATASTRG
                     LFALARDRKIPGTFAKTTSGGNPIAAATVVAVVSAAWVLATFGVEDLLALEGAPHELA
                     VFQWISTLGAFLIMVVYGVMALGAFSGLRDHPNKAGLIVSCLLGIGVAAGAIFGAIYK
                     VAPPFDLVWVWGAIWAAIGLLVTLAVKGREPARRALADLSTGEEG"
     gene            18547..19965
                     /locus_tag="VF195_02140"
     CDS             18547..19965
                     /locus_tag="VF195_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017936108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6579657.1"
                     /translation="MTDPDRPLRLDAPREEALEHVAKLVAEAWRSFDRPRPQEPPLDE
                     RLRAILHAGLPAGPVSVHEALDDAARAMDESIAQPRPRYFAFIGSSGLEIGALGDLLA
                     ATYDMNLAVDARAATVLEQQAVRWVGEFIGYPARNGAFTSGGTISNMTALAAAREWAL
                     PGSRAAGLGGLRPAVYASAEVHYSITRAVELLGIGSDRLRDVPIDDEHRMRPDLLAEA
                     IDRDAADGVTPVAVVATAGTTLTGAIDPLASIAEVCETRGIWFHVDGAYGLPAASVVP
                     DRFAGVERADSVSVDAHKWLYVPKACGVALVRERDALSQTFAHEQGYLPHQQHELHAA
                     DITLEYSRPLRALKLWLAFRAHGAAQFREAIGRNLAEAQLLHRSAAERPDFETFDAPP
                     HLSIVPIRHVPAGVADLDAHNQALAEAIQADGRVYLASALIEGEVWLRPCFVNYRTTD
                     DDVMAILDVAGELGERIAKEGR"
     gene            19962..20474
                     /locus_tag="VF195_02145"
     CDS             19962..20474
                     /locus_tag="VF195_02145"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6579658.1"
                     /translation="MNDEALLPAERKILAELGHLPLDFRAMWAISNVFRSSTALRRHL
                     EATVLAPDRLSWTAFTALWVLWIWGEMESRDFASAVGISRPTATGVLSTLQRRRFVRR
                     KKAAEDGRVVLVSLTPSGRRRIEELFPRFNAEEAHLASVLPGEDQDRLAAMLRELLAG
                     VDTRSAATLP"
     gene            complement(20486..21874)
                     /locus_tag="VF195_02150"
     CDS             complement(20486..21874)
                     /locus_tag="VF195_02150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579659.1"
                     /translation="MDERNPSERPDASADAPDQYRPTDRLVARLAELRSPLEVLAPQD
                     QDKGREAIERIRQAQERIEALEEMLGKARDLEHELTAKAVRDRGQIFRYEAEISELSA
                     ISARVSTAEEARRQAEAVAAKSERALALAAAQVEAQSAEIVQLRSRNTELEADLVAVT
                     EKLAAAAVARAEAIRLKAERDEARERAQVERGLAAQDRRRAAEAGLRAAELQHQLREA
                     ERRMVQVSNTTNRESVAVPEDAVGEEVAEIPPPWIELQRASSEAASVGPHTAAVPDVV
                     EAASEQDEEPMVVKVGDAEPDVAERDEVAAVDEPSGAAAPTTPSGPVATPRSGSSAAE
                     PGSALPAAKMPAPPKPWADRASAASTAAPWEAITAKGTEGITSTGEGVVDEPRVTESD
                     DASADARDVEGIPPTTDVIDLTSVDQTSIDDLDEDEGDEDAGADSGSTSGGGWAAPNE
                     KIWQLLRGRRRD"
     gene            complement(21988..23235)
                     /locus_tag="VF195_02155"
     CDS             complement(21988..23235)
                     /locus_tag="VF195_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007379203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6579660.1"
                     /translation="MAAEHPEIDLANPDSFVDEVPYEWFDHLRIEHPVFWHPERSPNK
                     GFWAVTRYDDLTAIHMDWRSYSSELGAVSLEELDEEQLQIRKSMLETDPPRHTELRAI
                     CSKRFSARGVGKYEDWIRDVARDVLDRALPMGEFDFVPEISRDLPIRFLCSIFTVPQE
                     DAPQLISWGDQMIANQDPDLSRAVIDRDDTEAYRLLPFRSPAAYEVFDYADRQRDLRL
                     ADPQDDVINALTVAQSEGVLNEQEFHNYFGVLMIAGNETTRHTISHGMLALMEHPEQL
                     KLLQEEPDRIPRATEEILRWATPVHHFRRTATRDLELRGAEIKRGDKVVTWYSSANRD
                     EEVFPEPYTFDVTRKPNDHVTFGPGGPHFCLGAHLARLETKILFQELLPRLKSIELAG
                     PVERIRSDFVNGIKRMPIRIVAA"
     gene            complement(23459..24763)
                     /locus_tag="VF195_02160"
     CDS             complement(23459..24763)
                     /locus_tag="VF195_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6579661.1"
                     /translation="MDLSGRRLRLLISDILGLERGKYLFGDVAEAGHAAFCIGLYPLT
                     TDKEILPVRRQHFDVGLPDVEATMDRDTLRPGWEDDTVVGIADVTQHGVPLEVDPRHV
                     LKRAVRAWRELDLEPQIAFELEFYLCERADDGSWEPAHLPSHRVYGTGMSVDPSGAVH
                     DMVTASLASGFHVESWCSEFDSAAFEVNWRYRDAIAGADEAFLFRVLVREIAARHGRH
                     ATFLGRPFNDRGGSGMHVNLSFRRRDGSNAFHDSDDPDGLSDLARQSIAGLLAHHRGI
                     SAICAPTTNAYKRLKPDMLNGYLANWGFDDRTVGVRVPPARGEGSRIEHRMADAAANP
                     YLVGAALLHAARLGVTEELELGPAQEPGADPNTDVGVPNTLEEALEELRKDAKLAAEM
                     GDWLVESFVALKQAEWDRYVASGETSDTAVTSWELDYYLPFF"
     gene            complement(24828..25673)
                     /locus_tag="VF195_02165"
     CDS             complement(24828..25673)
                     /locus_tag="VF195_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6579662.1"
                     /translation="METRRILLDGVATTVTRAGDELVAPDGRRIPMDEPTHLPPIQPS
                     KIVCVHLNYESRRAEFLAELGPAPTFFHKPITSLNAHGGEVVRPARCRYLNYEGEIAI
                     VMGERCTHIAPEEADEYIAGYTIANDFGLHDFRDTDAGSMLRVKGSDTLCPLGPGLVT
                     DWDFHSKGIQTRVNGEVVQDGSTDEMIWDMRYLVADIARTITLLPNDVLLSGTPASSR
                     PVQPGDVVEVEVEGLGILRNFIVEGEVPIREDVGAQPSGSEEVVSTAFGGDWEFRGVR
                     APKRG"
     gene            25694..26608
                     /locus_tag="VF195_02170"
     CDS             25694..26608
                     /locus_tag="VF195_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579663.1"
                     /translation="MRWVVGTAEGLVGSEGELWLVHRAITSLAWDGHGWLVLVDGREV
                     LRVHGDEVEAVGEIGDEAGRCLLPTADGVLLGTAHARLGLLRDGAVTMLRSFDEAEGR
                     DEWYTPWGGPPDTRSLSASAHGALFANVHVGGILHAQVPDGVWSPTIEIDADVHQVLA
                     DPLDDSHVVAATARGLAETRDAGATWHFTAEGLHAPYARAVALDGDRLFLSASTGPRG
                     GRAALYRREPGADVFERCAETLPDWFPGNIDSYCLAAGEGAIAFGTEDGRVFVSGDGG
                     GSWREVLGENARVTCVGVAASEGPGTST"
     gene            26620..27408
                     /locus_tag="VF195_02175"
     CDS             26620..27408
                     /locus_tag="VF195_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HEX6579664.1"
                     /translation="MAELKGFLPPRSPEGRASLVPAPPWHYSGDVMTIEFRTDPGRVA
                     ALLPDGVEPAEEDPGAVAIVWADWQSCSDSGEELLDPVRSQYKECFVVVRCRYEGETY
                     SRCVYIWVDKDFALARGWYQGYPKKLGSIWMTRPVSVGRAGPRLGAGAVFGGTLQAND
                     RRLAEARLTLTGGSESAGFVNALPMLHSRWMPSIEKDNGDSLAELVTMRSRDVDLGPA
                     FTGDADIRLFDAPGEELAGLEPSEMIAGYWRQVGATFDGGSRLA"
     gene            27411..28172
                     /locus_tag="VF195_02180"
     CDS             27411..28172
                     /locus_tag="VF195_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013586292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6579665.1"
                     /translation="MRLARFRYGDRIATGAMDRGSEEIRVLRGTFFEDPLPTGETVAL
                     SDVHLLAPVLPSKLVCVGRNFVAHAEEWGLSVPEEPLLFLKPSTAVIGPRDPIQLLPI
                     SRRIDYEGELGVVIGRLARGVRAEEAFKYILGYTCGNDVTLRDLQKTDDQWARAKGFD
                     GSAPLGPWIETDLDPNDAIVRTRLNGEIRQQASTSDMVFGVATLIEYTTTFMTLLPGD
                     VLLAGTPEGVGKLADGDIVEVEVEGVGILRNEVRG"
     gene            28169..29593
                     /gene="gltX"
                     /locus_tag="VF195_02185"
     CDS             28169..29593
                     /gene="gltX"
                     /locus_tag="VF195_02185"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020574786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEX6579666.1"
                     /translation="MKVRCRFAPAPSGSIHVGNARSALFSWLYARHEGGTFVLRLEDT
                     DASRVTEEASQGVVEDLRWLGLAWDEGPDVGGPYGPYRQSQRSEIYREMADRLLEEGH
                     AYRCWCTAEELDERRKAALARGEPPGYDGRCRQRPAGEIAAFEAEGRPFAIRFLMPER
                     GWAVEDLVKGEVRWGSNDLRDFVIVRSDGSPVFLLAVAVDDLLMEITHVIRGDDLLAS
                     APRNAAVIEALGGTPPAYAHLPQVLGPDSKPLSKRHGDTSVAAFREHGFLPEAMVNYL
                     ALLGWAKDDHTTLLTRDELVEAFDLSHVSHNPAVFDVQKLEWMNNHYIQSLDEEELAA
                     RCVHFLTEEGLSLDPDLLRRAMPIVRERMKTLAEAPILLRFLFTDDVRLNEKATDLIA
                     KAPEGYLKEAAEALDALERWDSEHIQAALDTLAAAAGLSRTKGWQPVRAAVTGSNVSP
                     PLPESLELLGRERTLARLRPAARG"
     gene            29590..30351
                     /locus_tag="VF195_02190"
     CDS             29590..30351
                     /locus_tag="VF195_02190"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX6579667.1"
                     /translation="MTRRILFVAVAVVHAVGLTPAVGLTPTQLASPTAAVVAAEPLEP
                     DVASLRPRIVWKPIPYGARRRRQMAAYSRRHYGSWGWKLEAPVAIVEHYTTGTTWQGA
                     WNTFASNSKHLGEYPGTCTHFIVHTDGTIYQLASLGIRCRHTIGMNQLSIGIEHVGRS
                     DAEVLRNGSQMRSSLRLTLWLVARFGIEIRNVIGHGESLQSPLRYERYASWRCMTHSD
                     MSRSAMITYRGRLRDRARAADVPIGPAPAWVDIGC"
     gene            30393..30635
                     /locus_tag="VF195_02195"
     CDS             30393..30635
                     /locus_tag="VF195_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579668.1"
                     /translation="MSEQEAPVSKVLNLSERQDAEGSWHMVLKDVCGWCGCGYLVAED
                     DHDLVWEPGRHHDKHCSDDACECHTRARVGKRHDEW"
     gene            30794..31087
                     /locus_tag="VF195_02200"
     CDS             30794..31087
                     /locus_tag="VF195_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579669.1"
                     /translation="MTEDAMVGEDGSMTIESAAELMNVEVRSIRQWSAIGSLRIDVEL
                     VHLGQVKALARSPIVIGDRKENQRGGLRALLREATPVDAQSVAGLQALARERG"
     gene            31369..31947
                     /locus_tag="VF195_02205"
     CDS             31369..31947
                     /locus_tag="VF195_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005456114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2182 domain-containing protein"
                     /protein_id="HEX6579670.1"
                     /translation="MAALVAASAVAWVALVTYEPPMGFLGFTAGWSLMMAAMMLPSIA
                     PLWLLSRGSRVGMAVGYLVVWSAVGVVPYVAMERAMDMTVPVVLGLAGVYELSPLKSA
                     CLRRCRNPAAFLMEHYRSGAVRLGVAHGIWCLGCCVGLMAVLVLAASMQLVWAIVIAA
                     AVFVQKVLPRGEVFARLIGIGLLVAAAAVALT"
     gene            31958..32560
                     /locus_tag="VF195_02210"
     CDS             31958..32560
                     /locus_tag="VF195_02210"
                     /inference="COORDINATES: protein motif:HMM:NF018712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1326 domain-containing protein"
                     /protein_id="HEX6579671.1"
                     /translation="MGDVWSMKGTVVVACNCDYGCPCNFQSLPTMGKCEGGWTWHVDE
                     GAYGDVRLDGITFTLTVNWPGPIHEGNGVGLLLVDERADEAQRNAIHTLAKGEVGGPW
                     GVLGWTWPTLEGPEAVPYDLHLDGIRSSVRAGDVYELDSEPIRNPVTGVEITPGVMLP
                     QGTIFKEGLFGSSKRLRVDRLDMDYANKYTAVAAFDYRGP"
     gene            complement(32557..33039)
                     /locus_tag="VF195_02215"
     CDS             complement(32557..33039)
                     /locus_tag="VF195_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6579672.1"
                     /translation="MSEDRSYIEENTRERERMRAIVDRVSEDELRLSVNPHWTVAGVL
                     GHIAFWDARVLSLADKLERGEPFTISDTEPEDVDWINDASRALIHAIPPREAARLALE
                     IAEETDARVATLPVDRLWPRDPDSPLYALRASHRGEHLDELEAALRDPSGPKGDAPTI
                     "
     gene            33438..33707
                     /locus_tag="VF195_02220"
     CDS             33438..33707
                     /locus_tag="VF195_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6579673.1"
                     /translation="MKDNHESIVKRLRRIEGQVSGIRKMQESGRYCVHVLDQISAARA
                     GLEATALLILEDHVNGCVREALDDGKGEEKAAELLQAVGRFARSV"
     gene            33709..33843
                     /locus_tag="VF195_02225"
     CDS             33709..33843
                     /locus_tag="VF195_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579674.1"
                     /translation="MSPRDMALLTVPEPGFCGTFLVAARIAHVVGHTKPQQTERSRET
                     "
     gene            33881..34033
                     /locus_tag="VF195_02230"
     CDS             33881..34033
                     /locus_tag="VF195_02230"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6579675.1"
                     /translation="MSGRSATSSAPTSSWRATDAKTILEMRFARGEISREEFEERKRV
                     LESRAA"
     gene            34258..35376
                     /locus_tag="VF195_02235"
     CDS             34258..35376
                     /locus_tag="VF195_02235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6579676.1"
                     /translation="MSPRLVNAAVPVGRRNLLADRRRLLISVGGVGAALALILFLQGL
                     WLGFQRQITAYEDNVGADLFIAEPGTRSFLGESSTVSLESVAEIEAMLDVDRADAIVS
                     RGAVLDLHGRKQFASVIGSRPGGLGGPWALAEGRVSRALDEAVIDETLASQHSLTLGD
                     TIDILGAPFRIVGLSTGTRSWMFGFVFVTVDAAQRITRAPGSVSFVLVTTEHPTSVAE
                     GIRDELGLSTLSPEELRSNDRALLAKSIAGPIELMVVIAFAAGTLIVALTVYSQVIER
                     IREYGIAKAMGARAGRLFRIVLGQTMIVALLGAALAFPMFLLGAKLVNVLRPQFRADM
                     SAPVSLVVVGAATLMALLAALLPTRRVSRLDPASVYRG"
     gene            35379..36482
                     /locus_tag="VF195_02240"
     CDS             35379..36482
                     /locus_tag="VF195_02240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6579677.1"
                     /translation="MMRRVAIGRKNLFADRRRAALGVAGLAAALLLVLALDGIFAGAM
                     RGVTLYIDTSAADVFVAQRGVRNMHMTASSVPLRALDEVRAIPGVVWAQPIALTSDSL
                     EVGAQRQLANVIGYEVGEPGGPTEFERGRPPEAGEIVLDDRAAELLGVGLGDDVVTVG
                     LSWRVSGISSSMTNIVNSVAYVTFQDLVSARGMNETASYLLVKVAGSTDGVASRIEDS
                     TGLGALTRAAFSAEESQAVRDMSTELLAIMTLSAFIIALVVVGLTLYAGTLARLREVG
                     VMKALGAGAGPLGAAVLSQAIWTVAAATATAILLALGLAWALAGIGSTISVVIEPSSV
                     IRAAAGALVIGGLGAVSPLIKVLRVDPSSVFRR"
     gene            36485..37327
                     /locus_tag="VF195_02245"
     CDS             36485..37327
                     /locus_tag="VF195_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6579678.1"
                     /translation="MDDIAIRAEGLEKVYGEGDVEVRAVRGIDLEVRRGEVILIMGPS
                     GSGKTTLLSMLGAMLRPSSGRIVVDGEDLAGLSERHLPAFRAHRFGFVFQDFNLLAAL
                     TAQENVEFAQNLAGVTGRQARDRAEGLLRDFGLGERLRFRPEKLSGGEKQRVAIARAL
                     VNEPAVVLADEPTANLDSKIGHEIARLLRSAASEHDRSVVIVSHDARLKDIADRVLWL
                     EDGEFRSMSEMATDPVCGMAVEREGAPHYRWDGTTSFFCSPACRDEFATEPGRFAGAV
                     RAEA"
     gene            complement(37613..37903)
                     /locus_tag="VF195_02250"
     CDS             complement(37613..37903)
                     /locus_tag="VF195_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018349448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6579679.1"
                     /translation="MPTQTRGYTATKDQLLARLARIEGQVRGVARMVEEDRYCIDVVT
                     QINAARAALDKVALGLLDGHVRHCLVGGQGGPNDPDEQAEELMGAVGRMVSR"
     gene            38055..>39151
                     /locus_tag="VF195_02255"
     CDS             38055..>39151
                     /locus_tag="VF195_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6579680.1"
                     /translation="MTGTEILVTGSGVALIGALAYFFFGPKHAGRAEVRGGVQEVEIT
                     VKGGYSPSVIKVQQGVPLRLVFDRQERGECTSEVVFPDFRIRRGLPAFARTTVDLTPE
                     RSGEFGFACGMNMVHGTLVVEPAEGDVAGAPERVDTSVSGALAEAKAVDGHRHQETRT
                     VSMEPGGEAAGRVALALERGGGVACAACLRTIESELESLPGVRSVQTDVVNERASVEF
                     DPSAVPVEDMRDAVASIGYRVQQPPRAASDGTEDVEAAARRAEVRDLTWRVVVGAALT
                     APVLIAVMASEIFDAAWVPEFLMNRWIQLALTIPVFLYTGWPIHTVGWRTLLHRTAEM
                     NTLITVGTTAAFAYSVLVTVAPGLFPAEVREV"
BASE COUNT         5780 a        13065 c        13857 g         6449 t
ORIGIN      
        1 ggggcatcga cgatcgaggc gcaggacaag gcaggcacga cggtcgccga gatggtgcgg
       61 ctcgcgctcg aaggccaatt cgtgccgtac gccgtgaacg tctccgccgg cgccgaggtg
      121 agcgaggccg tccgaccgtt cgtcggtctc gcggagcgcc tcggatcgct cgtcgcgggt
      181 ctggcggacg cgggggttcg gtcgatcgtc gcgtcctacc tgggtcgcgt ggcggagcac
      241 gacacgcggg tgctcacact ggcgatcctg aaagggatcc tcggacgctc cgtgaacgaa
      301 cccgtgtcct tcgtgaacgc gccgttgctc gcgcgcgatc gcgggctcac ggtcagcgag
      361 atgcgctcga ccgggtcgca ggattacgtg agccagatct ccctgcgggc ccagacggag
      421 tccggatcgg agatcgacgt cggcggcacg ctgctcggca agaagaacgc cgaacgcgtg
      481 atccaggtct gggacttcga ggtcgagatg gagcccgcgc cgtacatgct cttcttcgtc
      541 tacgaggacc gccccgggat catcgggaag gtgggcacga tcctcggcga gcacgacatc
      601 aacatcgcca cgatggaggt gggccgggag gctgcggggg gcgacgcctt gatggccctc
      661 acggtggact cgcccgtgcc gccggacgtg ctgcgcgtcg tagaggacgc gatcgatgcc
      721 cacacgatcc gcgccgtcac gctcgacacg tgaccgaagg ttgccgcagc ctcgtccgtc
      781 ggggtggaca cccgtgttac cctggaacga ccatgaggcg gtacacgacg aaacgaccag
      841 tgcacggggc tctctgacgc gtctcacgcg tcgcgccccg tagtccgttc ttcgtcgacc
      901 ctccgcatca cccccgcaag gagaccgctc catgacccgc tcacccaagt ccaacgttcg
      961 gcgtctcgcc ctcggccgtc tgatctcggt catgggcggt gccgccgcct acacggcgct
     1021 gaactacacc gtctgggaac gcacccactc gccgttcatg caggcggtct cgctgctgct
     1081 gacgttcggg gtcgccggcc tcatcggtcc cttcacgggc gcgctgggcg accgatacaa
     1141 ccgtcggacg gtcatgatct ggtcggaggc catctcggcc gccttcttcg cggcgatggt
     1201 cttcgcgaag gaccccggcc tcctgatcgc cctcgcgttc ggatccgcga tcgccgagct
     1261 gccgttcttc tccgcctccc gtgcggcgat cccgaacctc gtcgagtccg agaaggatct
     1321 ctcgtgggcg aacagcctcg tgtcgatggg cgtgcacgct gggatcgcgg tcggcccggt
     1381 gatcggcggc ggactggtgg ccatcctcgc accgggggac acgccgctgc ccgaccggtt
     1441 gcacatggct ggcgcggtcg tcttcgggtt gaacgcgatc tccttcctga tctccctcgc
     1501 cgtcacgctg acggtccggg gtcgcttcga agaggagcgg tcgccggagg ccgaacccga
     1561 gcacgagggg ctgttcgcgg gcgccgccta tctgtggcgt gagcgggtcc ttcgcaggat
     1621 ggccttcgcg tggctcgtct tcgtgctcgg gatgggcatg gggatggtgg ccgacgcgcc
     1681 tctcgcggaa tccttcggga cgggcgggtg gggcttcggt ctcctgatcg cgtgctgggg
     1741 gaccgggtcc gtgctcggca ccgtgttcgg gcgatggatg aacgcccgca cggagcccct
     1801 atggatggtc ttcggggctg ccggcgtggc ggtgtcggcg ttcggtgtcg gcttgttcgg
     1861 gctgttcccg ctcgtgctcg tgtccctgtt ggtgatgggg acgtcagacg gcatctcgat
     1921 cgtggcggag aacgggatca tgcagcgtcg cacgccggat gtggtccgga gccgggcgat
     1981 ggcggccctg gaagcggtcc tgtccctcgg attggccatc gcctacctgg ccgccggtcc
     2041 ggtcctccgc ctcgtcgagg cacagacggt ctacgtgatc gccggcgtgt tcgccgtggg
     2101 ggcgaccatc gtccttctgc cgctcatccg tctgaaggcc gacgccagcg acggtaggcc
     2161 gaccggccca ggagtccccg tgcagcagcc gactaccggg gaccgcgcga ctgtgtgatt
     2221 cttgccctcg atgacggagg gcacagggcg cgcagcggtg cggcgggtcg cggcggcgcg
     2281 tggcatctcc ctgaccggga gcggggccgc gttcgcggcg ctggcgtata tcgtctatag
     2341 cctgaccgga ggatccgcgg tctggctctc ggtcaccctc ctgctcacga tgggcctgca
     2401 gggcgtcgtg cagccgttcg cgagctggct cggggaccgg ttcgaccgcc gacgagtgct
     2461 ggtgatctcc gatcttgccg cggccgccgg gttcgtcgcg ctcgccttcg ccaggacacc
     2521 cgggcaactc gtcatggtgg ccgcgatcac ggcggtgctc gaatcgccgg tgtgggccgt
     2581 ggcgggagcg tcgatcccga acctcatcga ggaggagcat ctcccgtggg cgaatggaca
     2641 ggtggcgatc gggaggcatc tcgggagctt catcgggccg ctcctcggga cgcagctgat
     2701 cgtctggctg gcgggcgagg ccccatcggt ggatcgactc ctcggcgccg gcgccttcgt
     2761 gttcgggatc aacgccgcga cgttcctcgc atccgcctgg ctgatcggca gcacggcggg
     2821 gaggttcaac gacgagcggc gcgaggagag cgagcacgcc gggatccggg cgggattccg
     2881 gtacgcgatg agcgaccggg tacttcgctc gatcctgatc ggctggtccg tgctcctgct
     2941 cggcgtcgga ctgatcctcg tggccgagct cccctatgcg ctcgggttcg gggagggcgc
     3001 gttcggctac gggttgatct cagcgttgtg gggaggtgga gccgccttgg gcgcgctgct
     3061 cgcggcgcga tggctcacgg ccgaccgtga gccgaggacc cttctggtct ccgtcctcct
     3121 cggaggtctc gtcatggtgg cgatcggatg gtcgccggtg tggatcctgg cgctcggcct
     3181 gatggtgacg gaaggtatct tcgaggggtt cgcgagcgtc tcggaacagg ggctgctcca
     3241 gcggaggacc cccgatgatg ttcggagccg ggttgcgggc gcggtggaag ccgcgaccct
     3301 catcgccctc gcgatctcgc tcaccgccgg agggccgatc gtggactggc tcggccctcg
     3361 cgccgcgtac tacctgggcg gcctcatgac cgtgggggcg gggctcgtga tggctgggcc
     3421 gcttctctat ccgggcctgc cacgtcatcg ggccgacgag gcgttcgtgt tcgagccggc
     3481 ggagcgcgtc gtctccgatc ggtccgcgag gacctaggag ctcggcagcc gggtcgcggg
     3541 gtccgggaac aggacgcgga aggaggcacc tccgccctcc cgatcctcga cccaggcacg
     3601 gccgtcgtgc gcctccgcga agcgcgccac gagggacagt ccgacgccca ggcccggtga
     3661 gggcgcggag cgctctcctc gctcgaacgg ctcgaagagg cggtcccgct cgtcctcggc
     3721 cactccgggt ccgtcgtcat cgacgcgcac gatcacgccg ttgtccacgc gtcgcgcccc
     3781 gacccagatc tcggacgtcg gcggagtgtg cttgatcgcg ttgacgagga ggttctccac
     3841 gatcctcacg accatcgatt cgtcgatcca tgcatcgatc ggctcggtct gcacgtggac
     3901 cgtccgctcc tccaggacgt cggactccgc gaccgtgcgg ccgagaagcg ctcccagatc
     3961 catcagctcg cgccgcggct gaacgaggcc gcgcgtgagg cgatccatat cgaggagatc
     4021 gttgaccatc gccgtcaggc gccgggactt gcgggcgagg ctccggacca gctggcgacg
     4081 gtcctcctgg gagatcccga gctcgtccgc actctccagc gtgatcgcgc tgccgaggat
     4141 cgccgacaac ggcgtgcgga ggtcgtgcga gacggcggtg aggaacgtgt tcttcatgtc
     4201 gtcggcctcg cgcagtcgct ctacggcctt gcgctccatg tcgagggcct gcgccagctc
     4261 ccgctcggcc cgcttctgct cggtgacgtc gtacatcacg ccttggtaga agagcggctc
     4321 gcccgactca tcgcggatcg gcacggtctc gtcgtggacc cacacgagtc ttccgtcgcg
     4381 cgcgaccatc cgatagtcct cgctgaacgg gtgctcgtcg ttcgcctgct cctcggccag
     4441 gagcacccgg tctcgatcct ccgggtggag cagctcgcgc cagaggttcg gacgcgcctg
     4501 ccgctcctcc ggtgtgtacc cgaagagctc ctgatacttc gggctgatgt agatcgggct
     4561 gtcgccagaa cctccctctc cctcgatgta gacgactgcg gggatctgct cggcaagcga
     4621 cttgaacttc acggcctcgg accggagctc ggactcgagc cgcttctgct ccgagatgtc
     4681 gacgtagagt ccctggacgt acgcggcgtg gtcgtcctcg tccttgacga ccgtgccctg
     4741 gtcacggatc cacacgatgc tgccgtcggg ccggatcatc cgatattcga gcgtgaatgg
     4801 ctccgcccgt cggatcgcct cgcggatccc tcgcaccgcc tcgtcacgat cctcggggtg
     4861 caggtgcccg gcccacgcgt cgtcgctgat gaacgcatcc gcggagagcc ccagcacatt
     4921 ctcgacctgc gggctcacgt agatcgaggt cagatcgtcg tcggcggtgt ccatgtacgt
     4981 cacggccggg agctgttcca ccaggttgcg gtagcgtcgt tccagctcct cgagctgcag
     5041 ctcggcctcc ttccgcttcg tgatgtcgaa gaagaacccg aggacgtatc cgggttcccc
     5101 tgcgtcgttg cgcacggtgg tcgccgagtc gcgtacccag atcacccggc cgtcgtcccg
     5161 caccatgcgg tactcgagcg tgaacggctc gccggagcgg acgccctcga cggtttcgtg
     5221 acgggctcgg tcgcgatcgt cgggatggac ccgttccgcc cactcctcgc ccgtcgagcc
     5281 cgagggtctg ccgaggaggt cgttgacctg cgggctcacg aagaggttcg cgaggctctc
     5341 gtcgggctcg tcgatgtagg tgacgatcgg gagctgctcg accagggtcc ggtagcggtg
     5401 ctcgacctcg gcggcgcgtg cctcggcctc cttctccttg gtgatgtcca cgatcaggcc
     5461 gatccagtgc gccgacctcc cttccgacgc gtcgacgtgc acggcctcgt cccggaccca
     5521 gatcacgcgt ccgtccacga cgaccaggcg gtactcctcc agatacgggt ccttctcggc
     5581 gtccgccacg ccgtcggaga gctccacccg ctcgcgatcg tctggatgga cgagggagta
     5641 ccagaactct cgagactcgg tgaagcgctc gggcggatac cccgtgagcg cctcgatccg
     5701 cgggctcatg tacgccatgg tgcccgtcgg cccttcggtc tcgacgtagg ccacgccggg
     5761 catggacccg agcacgtgat ggaagctcag gtcctggatc gggaagtcgc gcgcgttcag
     5821 cggcgtcggg ctcgtcatag gtcgctcggt gggtgttcag gttccgtagg aagcatcggc
     5881 gctcccccgc ccgcacgtga atggtccttc cgagcccggc acgttgggcc catccggcat
     5941 cccccgctcc ctcaggggcg ttcgcccggt aacgcgctct ggggtaccac ctcgggatcg
     6001 cctctcggga taagggcccg gaaggaggcg ccgccgccct cgcgttcctg gacccacgcg
     6061 cgacccccgt ggagctcggc gaacttggcg acgagcgaga gcccgatgcc aacgcccggg
     6121 ttgtgcacgg aggtcatcgg tccgtgctcg aagggctcga atatcgactg ccgatgctcc
     6181 tcggggatcc ctgggccgtc gtcctccacc tcgatcagga cgtggtcgcc ctcggggagc
     6241 accttgaccc aggcctgaca ctcggacggt gtatggcgtg ccgcattcat cagcaggttc
     6301 tcgacgatcc gggagatcat ctgcggatcg acccaggcgc tcaccgaccg cgtgtcgacg
     6361 tggacctggc gcccggccat cgcctcggag tcccagacga ggagagagat gagcctgccg
     6421 acggcgatct cctgcagctt gggctcgacc acgccgcgtg cgagacgctc cgtatcgagc
     6481 aggtccgtca cgatggtgtt gagtcggcgc gccttggccg cgaggctgtg gatcagctgc
     6541 ttgcgctcct cttcggtgat gccgagccgg tcgccctcct cgagcgtgag cgcgttgccg
     6601 aggatcgccg acaatggcgt tcggatgtcg tgcgagaccg cagtgaggaa cgttgtcttg
     6661 atctcatcgg cccggcggat gtcgtcggcc gccagtcgtt cgagatcgag cgcccgcgtg
     6721 agatcctccg ttgcggcctt ggcttcggag atgtccatca tcacgccctg ccagtgcacc
     6781 ggcgtcccct cgtggtcgcg caggagcacg gcttcgtcgc ggacccagac cgtgcgccca
     6841 tccgctgaga gcagccgata ctcgccggag tacggttcgc ccgacgcgtc gctgcgggac
     6901 cattccgcga cgacgcgttc gcggtcgtcg ggatgcaggg cgctcgccca ccaccccccg
     6961 tcacccgtcg cgacctccgg cgggtatccc agcatcgtct cgatgaacgg gctcacgtag
     7021 gaaggagcgg tcggttccgg gccaacgggg tccacgtaga cgatcgcggg gacgcgctcg
     7081 accagggatc ggtacggcag ttcgaccacg tgatcagccg ccgcgtcgtt ctccgacctc
     7141 ttcgcgctca tcggtgtcac ggggctgaga cctccggttc ccccatcgga ccgacgcgtc
     7201 acgtccttga cgtccgttgc atcgtaggct ccggcggacc cttcacgagg agcatcgatg
     7261 ccggagacgt tcacgttcta catccagccc tggtaccgga agtccccgta cttcgaagcc
     7321 accaaacgtg ccgggtgcgc gagctggggg ctgtacaacc acatgctgct cccgacgctc
     7381 tacgacgacc cggtgaccga gtactgggcg ctccttcgcg gcgtgacgat gtgggacgtc
     7441 tcggtcgagc ggtgcgtcga gatctcgggg ccggacgcgc tcccgttcac gaacctcctc
     7501 acgtgccggg acctcacctc gtgcgaggtg gggcagtgca agtacgtcct catcaccgat
     7561 cgcgagggcg ggatcgtgaa cgatccggtc ctgctccgca tcgccgacga ccggttctgg
     7621 ctcgcgctcg ccgactccga tgcactcctc tacgtcaagg gcgtggccgc gctcgccggg
     7681 atggacgtcc gggtgatcga ggcggacgtc tcgccgatgc aggtccaggg cccgcgctcg
     7741 aaggaggtcg tgcgcgatct cgtcggcgac gaggtcgccg cgctcccgta ttaccggtgc
     7801 gccgaggccg agatcgacgg catccccgtc gtggtctccc ggacggggtg gacgggagag
     7861 gtgggctacg agctctatct ccgcgacgcg gcacgtggcg acgagctctg gcgtctggtg
     7921 gaggaggccg gcgcgccgta cgcgatccgc gcgatcgctc cttcggaagc gcgccggatc
     7981 gaggccggga tcttcaacta cggctccgac atgcgcacgg aggacacacc gttccacatc
     8041 accggactcg agcggctcgt cgaggaccaa gaagccgact tcatcgggaa ggacgcgctg
     8101 cgccggatcg ccgaggaggg ggtggatcgg aagctcgtcg ggatcgatat cgagggcgcg
     8161 ccgatgaccg acgagggcgc cctgaacgac ttctggcccg tgcacgagtc aggaggcgat
     8221 cgggcgtggt cgaggatcgg caaggtcacg gcgggcgcgt ggtcaccacg cctcgagcgg
     8281 aacatcgggt acgcctgggt gccggcgacc cacagtgcgg tcggcacgcc gctcgccctg
     8341 gaggctcgcg acgggaagcg ggatgcgacc gttgcgacac ttccgttcgt cgacccgacg
     8401 aaggagatcc cgaagtcgtg agcggggggt cgatgcgttt cgaccgggct gtcgagtact
     8461 acgaccgcac gcgtggcctg tccgaggacg cctcgcggga gatgaccgcg ctcctcgcgg
     8521 gcgagctcca cgatcgcggc cggacgctcg agatcggcgt gggcacgggg ctcgtcgcac
     8581 tgccgctcgc ggcggcgggg gtgcggctcg tcggcctcga tctatccgcg ccgatgctcg
     8641 ccaagctcgt ggagaaggcg ggtgggcggc tcccgttccc gctggtggtg ggggacgcga
     8701 cgacgttgcc gttcgccgat caccggttcg gcgcggcggt ggtccgccac gtcctgcatc
     8761 tggtgccgac gtggcgacag gtcgtggcgg agctcgcccg cgtcgtcgca gcaggcgggc
     8821 tggtgctggt ctcgagcggt caggtccccg agccgtggca ccaggtgacc gaccggttca
     8881 tggaccgggt cggccgcccc tccttcgcag atgcgctcga ttcgtgggag accggtgcga
     8941 tcgtgcaggc cttccgggcg cacgcagccg aggctcggca gctacctacg atcacccaac
     9001 aggttgcgca gacgatgggg gagttcctcg atcagatggc ggccgggatg cactcgtgga
     9061 catgggaggt ggaggaggag aggcgacggg aagcggtcgc ggagctccgc gcgtgggcgc
     9121 tggagcgctg ggggtcgctg gatccacctg ggggtcggga cgtcgcgatc gagtggctcg
     9181 cgttcgacct cccctgacgg gcgccgggta tcctgagagc ccgccaacgt cgtcgaggag
     9241 ggaaccgggt gccgatcacg caggtcgaga agatctggat ggacggcgag ctggtgccgt
     9301 ggcaggacgc tcaggtgcac gtgctgtccc acgccgtgca ctacggcagc ggcgtcttcg
     9361 aggggatccg ggcctacgag acctcgcggg gcgccgcggt ctggcacctc gacgagcacc
     9421 tgaagcggct gttccgctcg gcgaagctct accacaccga tatcccgtac tcccttgagg
     9481 cgatcacgca ggccacgaag gacgtcgtcc tcgccaacga gctgaacgcg tgctatatcc
     9541 ggccgctcgt gctccggggc tacggcgaga tgggcgtgaa cccgttgcag gccccggtga
     9601 acgtcgtgat cgccgtgtgg ccgtggggtg cctacctcgg tgaggaagcc ctcgagcagg
     9661 gcgtgcgcat caagatcagc agctggcgtc gcaactcgca gaactcgttg ccggccgctg
     9721 cgaaggccac cggtcagtac atcaactccg tcctcgccaa ggtggagagc ctcaaggccg
     9781 gctacgacga ggcgatcatg ctgaacgagg ccggcttcgt gacggacggc tccggcgaga
     9841 acgtgttcgt ggtgcgggac ggcgttctca ccacgccgcc catctcggcc gggtgcctgg
     9901 acggcatcac gcggggctcc acgatcacgc tcgcccgcga tctcggctac gaggtccgcg
     9961 aggagaacct cgtccgcacg gatctctaca acgccgacga atgcttcttc accgggactg
    10021 ccgcggagct cacgccgatc cgtgaggtgg acgaccgcgt ggtgggtgaa ggccatcggg
    10081 ggcccgtgac gaaggagctg cagggtgcgt tcttcgcagc caccaaaggt gagaacgaga
    10141 agtacgtcca ctggctgacg tacgtgaacg actgatgggg gagtccgaag agcgataccg
    10201 gctgccccgg acggtcgttc cctcgcgcta cgacctgacg ctggagcccg acctcgacgc
    10261 agggacgttc cgcgggagcg tcgacgtcgc gctcagggtc ctcgagtcgg tcaccgagat
    10321 cgtcctgaac gcgaacgagc tgcggctcga gggggccacg ctcaccgcgg cggacggcgg
    10381 tcactcggtg gaggtctcga agatcctcac cgatgacgac gccgagcgag cgaccatcga
    10441 cctcgccgaa gccgcagacc cgggggcctg gacgctccgg atcgtgttcg cgggcgagct
    10501 gaacccgcgc ctcaccggct tctaccggag cacgtacgcc gacggggatg gccgcacgca
    10561 ggtggtcggc accacgcact tcgaggccac ggacgcccgg cgtgcgttcc cgtgctggga
    10621 cgagccggat ctcaaggccg tcttcgcgac gacgctcgtc gtgaaggagg ggctgaccgc
    10681 cgtctcgaac ggcccggagg tggaacggga ggcgctgggc gacggacgga tgcggatccg
    10741 cttcgccgac acgatgaaga tgtcgacata tctggccgct tgggtcgtgg gtccgctgga
    10801 actcaccgag cccgcggacg cgcggggcgt tccggtgcgg atcgtccatg tccccgggaa
    10861 gggcgccctg acgccgttcg cgctggacgt gtcggtccac tcgctgaatt ggttcggcga
    10921 ctactacggc atcccgtacc cggaagcgaa gctggacaac atcgggctgc cggacttcgc
    10981 gcagggcgcg atggagaacg tcggctgcgt gacgtatcgg gagcaggtcc tgctgctcga
    11041 tccggctcgc tccacgcaag aggagcagct cggcgtcgcg gagaccgtgg cccacgagct
    11101 cgcccatatg tggttcggcg acctcgtgac gatgcgctgg tggaacggga tctggctgaa
    11161 cgaggcgttc gccacgttca tgtcgatgct cgcggtcgac gacttccgtc ccgactggga
    11221 gatctggaac acgttcacgc gtgaccggtc gaacgcgttc gaggtcgacg cgctcgaatc
    11281 gacccgccct atcgaatacg aggtccgttc gcctgacgac gcgagcggca tgttcgacac
    11341 cctgacgtac gtcaagggcg gcgccgtgct gcggatgctc gagcagtggc tgggcgcgga
    11401 ccggttccgc gacgggatcc gtcggtacct cggcgagcac gcctacggga acacggagac
    11461 gcacgacctg tgggacgccc tcgaggagga gacgggggag ccggtacgcc gaacgatgga
    11521 cgcctggatc ttccaacccg gttacccggc gatctcggtg agcctcgacg gcgaccgtgt
    11581 gcggttctcg caacgtcgct tcctcccgtc gcgagacgac gatgacacga catggccggt
    11641 gccgctgacg gttcgacagg tgagcggcga gcaggatcag gtcgaccgga tcctgatcga
    11701 gggcgacggc gcggagatcg cgttgctcgc acccgatgcg gtcgtggtgg cgaacgcggg
    11761 ttctgcgagc ttcgtccgcg tctggtacga cgacgagctg cgctcgcggg tagtgggcag
    11821 agcgatggac gtgctgtcgc cgatcgagcg ctacagcctg gtggacgatg cgtgggcggc
    11881 cgtcgtcgcg gggcaggctt ccgccggttc gtacctcgac ctcgtgcagg ggttcgcgga
    11941 cgagacggat ctccgtgtgt ggcagtcgat cctcggcggg ctgggctggc tggaccgctt
    12001 cgtggacgac gagccgcgcg agcgcctgcg ggcgttcgtg cgcgagctcc tccggccggc
    12061 gctcgctcgg ctcggctggg acgcgtcgcc cgacgagacc gacctcaccc gcgccctccg
    12121 cgggcagctc gtgcagtccc tcgcgatctt gggagccgac ccggagacgc tcgcgcgatg
    12181 ccgcgacctg gagctcgagg gccagggcga tccgcagctc ctgcaggccg ccgtggaggc
    12241 ggtggccgcg gagggcaccg aggacgacct cgatcgctac tgggagcgtt atcgcaacgc
    12301 caccacgccg caagaggagg agcgctacct cttcgcgact tctcgcttcc ccggtgacgc
    12361 ggaggtcgag cggatcctcg gcgcgagcat gacggacgac atccggaccc aggacgcgcc
    12421 gtacctgctc gcccggtcca cgaccaaccg gcgccagggg ccgaaggtgt ggcggttcat
    12481 cgccgagcat tgggatgcga tgcaggatcg gttcgcgtcg tccaacatca tcggcttggt
    12541 ctcggggatc cggtacctca cggatccaga gtccgtcgcg gacgtcgacg cgttcttccg
    12601 cgagcacgac atcccgcaga accacctgat gctccgtcag ggactcgagc ggatgcgcgt
    12661 gaacgcggag ctccgccggc gcgtgacccc ggagctgttc gagcgcttcg gcggctagtc
    12721 cggatcccga tccgtcacgc tcggcgcctg ccaaggcatg gtcctggcga acgaccccga
    12781 tcccgaggaa cccatcgcgg tgctcggggc gtccaacggc tttcggaccc atccgaaagg
    12841 agtcctccat gcggaaccga gccgctcgcg ccgcagctgt cgcgatcctc accacgatcc
    12901 tgctcaccgc cgcggctggg agcgccctcg cctgcggcgg gctggtcacg ccgaacggcg
    12961 cggtcagcct gctgaagacg acgacgctcg cggcctacca cgacgggatc gagcactacg
    13021 tgacctcgtt cgagttcgcc ggcgaaggtc aggaggtggg gtcgatcatc cccctccccg
    13081 gcgtcccgac cgatgtgatc aagggcggtg actggaccct ccagcgcttg atcctcgaga
    13141 cccagccgca actggaggcg ctccgggcga attccttcct cgcgctcgac agcgccgctg
    13201 gcgccgaggt cctcatgacg aagaagatcg atgccctcga catcaccgtg ctgaaggggg
    13261 gcgcggtcga ggtcggggac tgggcgcgcg agcacggcta cttcctccca ccggacgccc
    13321 ctgaggtcct cgcgttctac gccgagcgga gcccgatctt cctggccgcc cgcttcaacg
    13381 cagagcgtgc cgcgaaccgc ggcgtgcaga ccggccaggg cacgccggtc cacatcgtga
    13441 tcccgacgcc aagcccgtgg gtgccgctcc ggatcctggc gctcggccgt aaggcctccg
    13501 agatcgtgca ggcggacgtc tacctgctca ccgaccggcg gccggagact cttccccagg
    13561 ccgagcgctc gaaggggttc gaccagagcc agcgcggctt gatccaagag gtgagcgagc
    13621 ccgcgtcgac gacgctcctc gccgacctcc gatccgaccg cgggatgaag tggctgcccg
    13681 agagcgacat gtggctcacc tacatccgca tcaacgagac ggccggcaac ctgaggcacg
    13741 acctggcgat cgacgcgagc gggtacggga ggccgagccc cgtggccgcc ggctaccccg
    13801 ttcccgcggg cgccgcgccg tggtcggtcg tctggaccgt gatggcagcg ctcgccgcgg
    13861 tcgtcgtcct gatggcgatc acggatccgg ctgccaggcg caccctctcc cgggtcttcc
    13921 ggcggtgaag ctgcgggcga cggcggccgc ggtggcgctg gtgctcctgg tcggagcggc
    13981 agcggcatcc cgcggcgccg ccgcccgcgc caacgacggc gcgatccggc tcacgatcca
    14041 ctactcgaag ttcgatccag ccgatctcgc cgtcgagccc ggcgagacgg tccgcttcgt
    14101 gatcgtgaac acggacccga tcgaccacga gttcatcctc ggggacgagc agttgcagct
    14161 cgtccacgag gagggaacgg aggcccacca tgccccccgg cccggagagg tctccgtgcc
    14221 ggcgggggcg atcggcgtga cgaccttcac gttcccgagg atcagcctct cgctgatctt
    14281 cggctgccac cttccggggc actacgattt cggcatgcgg ggtctcgtca cgatcggcta
    14341 ggggtcgagg gtgccgccag acgatcgaag cgtgaggatc ccacggaccc cgaccacgac
    14401 acccaggagt acccacagac gatcgatttc ctcgatcgtc gctccatccg gcgatcgaga
    14461 cgaggatcag ccggcggctg agcccagggg gatctcaccc agccacggac gaccacccat
    14521 cctcgaagcc tagatgggaa ccgaggacgg tttgacaagg ctcgcgggtc gtccataccg
    14581 ttcgggtacg aacgagtctc ggggaggacg gaactgagcg ggcgcgtcga ggtcgaaggc
    14641 gtcgaggtct cgaccgacca ctggatcggc ggcgagcgcg tcccctctcg ctcgacgttc
    14701 accgatatct cgccgatcga cgaagggccg atcgcggagg ttgcccgcgg cggcgtcgac
    14761 gaggcgaacg ccgcggtcga cgccgccaag aagggcttcg agacctgggg ggcgatgtcg
    14821 ctcaaggaac gcgcggagat cctccatcgg gttggggagc tgatcgagga acgggcgccg
    14881 gatatcgcaa gggtcgagac tcgagataac ggctcgttgc tccgctcgat gacgaactcg
    14941 gtgatcccgc gtgccggcta caacttccga ttcttcgccg accatctcga caagctcgac
    15001 gagctcgagg acctcgacac gcgcggtcat tcgaaccacg tgagctggga tccctccggc
    15061 gtcgccgttg tcatcacgcc gtggaacgcg cccctgatgc tcgccacctg gcgcatcgcc
    15121 cccgcgctcg ccgcaggtga cgcggtgatc gcgaagccgc ccgagtgggc accgctgacg
    15181 gcctcgctgc tcgccgacat cacacgagac gccggcatgc cggacggcgc cttcaacgtc
    15241 gtccagggga tcggggaaga ggcgggcgcg gcactggtcg ccgatcccga cgtcggtcgc
    15301 gtcgcgttca cgggatcagt ggcgactggc cggctcgtcg cggcctccgc cggcgcgaac
    15361 ctgacgcctg tgtcactcga gctcgggggc aaggcgccgt tcctgatctt cgcggatacg
    15421 gacttggaag ccgcggtgac gcaggccacg aaccagttcg acaacgccgg gcaggtctgc
    15481 ctcgcgggca cacgcctgct cgtcgaggac gagatcttcg acgagttcct cgcacgattc
    15541 acagaggcgt ccgcggccat ccgccagggc gacccgcgcg aggagacctc cgacatcggc
    15601 ccgttgatca cgcgggagca cttcgagcgc gtcgacggct tcgttcaacg cgcgaaggcg
    15661 gagggcatcg aggtcgcgtt cgggggagga tcgaacgagg agctgggagg cctgcattac
    15721 cggccgacgg tgttcctcga gccgccgtca gggtccgaga tccttcagca ggaggtcttc
    15781 gggcccgtgc tcacgcttca gcgtttcacc ggcgaggaag acggcatcgc gaaggcgaac
    15841 gacacgacat acggtctctc ggcgatcgtc tacagcggcg accaggacaa ggcggatcgc
    15901 gtctcctcgg cgctcgtggc cgggaccgta tgggtcaact gcttctacgt gcgcgacctc
    15961 cgcgtaccgt tcggcgggtc gcggaacagc gggatcgggc gcgagggcgg tttctggagc
    16021 ttcgacttct acgcggacgt gaagaacgtc gtcaccgcac cctgggacct gaccaagacc
    16081 tgagaggagc accgaccatg ggagagatcg tcggcgcggg gatcgtctcg cacgtcccca
    16141 cgatcatgct gccggaggaa acccggctcg agatcaacga cggcaaggag atcacgctcg
    16201 ttcccgggct gcgacggctc cggtccgagg tcctcgaccg cgtcggggcc gacacgttcg
    16261 tcgtgatgga cacgcactgg gaggtgacgt tcgagcacat catcacggcg cacgagaagc
    16321 gcgagggcat cttcacgtcg cacgagctgc cacgcgggat gcggcagatc ccctacgaca
    16381 tgccgggcga tcccgagctc gcgttcaccg tctcccagca ggccgagggg cgcgacgata
    16441 cctgggtgct cgcctccgac gatccgtacc tcccgatctt ctacggcacg gtcaacatct
    16501 ggtcctacct gaaggaggga ccggagaact gggtgagcgt cggcatcaac cagtgcggga
    16561 ccaccgaaga cttcctcctc ctcgggcagc tgatcggtga ggcggtcctc tcgctcgatc
    16621 gcaaggtggt gctgctggcg agcggcggcc tctcccaccg gttcctgcct ttccgcgagc
    16681 tccgcggccg ggagagctcc gaccccatga agaacatcat cacgcccgag gcgcgcgagg
    16741 cggacctcca cgtcctcgac ctcctgcaga agggggatca cgccgcggtg atcgattggg
    16801 tgcccgagta caggaaggtg ggcccggagg gcaagttcgg gcactacctg atcatggccg
    16861 gggcgctcgg gggcgtggcg tgcacggcgc cgggcgagct gttcagcgag tacgaggcct
    16921 cggtcgggac cggccaggtg cacgtgtggt tcgaacgccc ggcgcccggt tggaccggct
    16981 cgaaggaagc ggcatagggt tcgcgccagg gatcggcgga cgagaggggg agttgcgtgg
    17041 caacggagac ggacaggata ggtggggatc ccggctacga gcgactcgga cgtcgacgac
    17101 tcaagctcgt cgacgtcgtc gcgcagtcgg tcggactgat cggccccgtg ttctcggctg
    17161 cgttcctgat cccgctgatc gccgggttca acttctcggg cgagggcgcg gggatcgcga
    17221 cgccgttcgc ggtgatcctg gcgtcgatcg gtgtcctcgg gctcgggtgg atcgtggccc
    17281 agtacgcgaa gcgcgtccac gctgcagggt cgctctacga ctacgtgacg atggggctcg
    17341 gctcccggat cggcggctgg gccggctggg tgtactacgg cggcacgctc ctgctgtcgt
    17401 cggcgatcgc ggtcctcgtc ggttggttcc tccgcgacaa cgtgctcccg gccttcgaga
    17461 tcgacccgat catgccgacg tgggcgtggt cgctgatcta cgtcgggatc gtgttcctga
    17521 tcctcgccgc cggcgtgcag atctcgacac gcgtgcagct cctcctcgcg ctcgtgtccg
    17581 tcgccgtcgt cctcgcgttc ttcatcaacg tgatcgtcaa cgcgcccgag aacagcgtga
    17641 aggccttcaa ccctgcggag gcgccgagct acacggggat cctgttcggc gttctgtacg
    17701 gcgtgttgat cttcgtcggg ttcgagaccg cggcgaacct cgccgaggaa gccgaacagc
    17761 cgaagcgcgc gatcccgaag gctgtgatgc tgtcggtggg gatcgtcgcg gtgttctacg
    17821 tgatcgctgc gtacgcgcag gtcgcggggt tcggcttcga cgtcagcgtg ctcacgtcgc
    17881 cggaggtggc ggcggcaccg ctcttcgcgc tcggttcgcc cgacgcggtc ggcggcatgg
    17941 gcggctcgtc ggacacgatg ttgaagatcc tcctcgtggt ggtgctcctc gatgtcatgg
    18001 ccgtggggct cggcgcagcg acggcctcca cgcgcgggct cttcgctctg gcgcgggatc
    18061 ggaagatccc gggcacgttc gccaagacga cgagcggggg gaacccgatc gccgcggcca
    18121 ccgtggtcgc ggtggtctcg gccgcatggg tgctggcgac gttcggggtg gaggacctgc
    18181 tcgcgctcga gggcgcgccc cacgagctcg cggtgttcca atggatctcg acgctcgggg
    18241 cgttcctgat catggtcgtg tacggcgtga tggccctcgg ggcgttctcc ggcctccggg
    18301 accatccgaa caaggcgggc ctcattgtct cctgcctcct ggggatcggc gtggccgcgg
    18361 gggcgatctt cggcgcgatc tacaaggttg cgccgccctt cgacctggtc tgggtctggg
    18421 gcgccatctg ggcggccatc ggtctgctcg tcacgctcgc cgtgaaggga cgtgagccgg
    18481 ctcgcagagc gctcgcggac ctctcgacgg gcgaggaagg ctgagggagg cttcggcacc
    18541 caggagatga cggacccgga ccggccgttg cggctcgacg cgccccgcga ggaggcgctc
    18601 gagcacgtgg cgaagctggt ggccgaggcc tggcggagct tcgacaggcc tcggccgcag
    18661 gagccgccgc tcgacgagcg gctccgagcg atcctgcacg ccgggctccc cgcggggccg
    18721 gtgagcgtcc acgaagcgct ggatgacgcg gcgcgagcga tggacgagtc catcgcgcag
    18781 ccgcgtccga ggtacttcgc gttcatcggt tcctccggcc tcgagatcgg agcgctcggc
    18841 gacctgctcg ccgcgacgta cgacatgaac ctcgccgtcg atgcgcgggc ggccacggtc
    18901 ctcgagcagc aggccgtccg atgggtcggc gagttcatcg gctacccggc gaggaacggc
    18961 gcgttcacga gtggcggtac gatctcgaac atgaccgcgc tcgcggcggc acgcgagtgg
    19021 gcgctgcccg gctcgcgcgc ggccggactc ggcggtctcc gcccagccgt ctacgcatcg
    19081 gccgaggtgc actactcgat cacccgcgcg gtcgagctgc tcgggatcgg gagcgaccgg
    19141 ctccgcgacg tcccgatcga cgacgagcat cggatgcggc ccgatctgct ggcggaggcg
    19201 atcgatcggg acgccgccga cggcgtgaca ccggttgccg tggtcgcgac cgcggggacg
    19261 acgctgaccg gcgcgatcga cccgctcgcg tcgatcgccg aggtctgtga gacgcgtggg
    19321 atctggttcc acgtcgacgg ggcgtacggc ctcccggccg cctcggtcgt gcccgatcgg
    19381 ttcgccggcg tggagcgcgc cgattccgtg agcgtcgacg cgcacaagtg gctctacgta
    19441 ccgaaggcgt gcggcgtggc gctcgttcgc gagcgcgacg cgctgtcgca gacgttcgcc
    19501 cacgagcagg gctatctgcc gcaccaacag cacgagctgc acgccgcgga catcaccctg
    19561 gagtactcgc gtccgttgcg ggcgctcaag ctctggctcg cgttccgggc ccacggagcg
    19621 gcgcagttcc gcgaggcgat cggccgcaac ctcgccgagg cgcagctcct ccaccgctcc
    19681 gccgccgagc gaccggactt cgagacgttc gacgcgccgc cccacctctc gatcgtcccg
    19741 atccgccacg tgcccgcggg tgtggccgac ctcgatgccc acaaccaggc cctcgcggag
    19801 gcgatccaag ccgatggtcg cgtctacctc gcctccgccc tgatcgaggg tgaggtgtgg
    19861 ctgcgcccct gcttcgtgaa ctaccgaacg acggacgacg acgtgatggc gatcctcgac
    19921 gtggccgggg agctggggga gaggatcgcg aaggagggtc ggtgaacgac gaggcgctct
    19981 tgccggccga gcgcaagatc ctcgcggagc tcgggcacct cccactcgac ttccgcgcga
    20041 tgtgggcgat ctcgaacgtg ttccggagct cgacggcgct ccggcgccac ctcgaggcta
    20101 ccgtgctcgc gccggaccgg ctctcctgga ccgcgttcac ggccctgtgg gtgctctgga
    20161 tctggggcga gatggagtcg agggacttcg cgtcggcggt cggcatcagc cgtcctacgg
    20221 caaccggggt cctctcgaca cttcagcgcc gtcggttcgt gcgtcggaag aaggcggcgg
    20281 aagacgggcg ggtcgtcttg gtctcgctca ctccgagcgg tcgccgacgg atcgaggagc
    20341 tcttcccccg gttcaacgcg gaggaagcgc atctcgcatc cgtcctaccc ggcgaggacc
    20401 aggaccgtct cgccgcgatg ctgcgagagc tcctcgcagg ggtggacacg aggtccgcag
    20461 ccaccctccc gtagtcgaag cgacgtcagt cccggcgtcg tccgcggagg agctgccaga
    20521 tcttctcgtt gggggcggcc cagcctccac cgctcgtcga tccggagtcg gccccggcat
    20581 cctcgtctcc ctcgtcctcg tcgaggtcgt cgatcgatgt ctggtccacc gaggtcagat
    20641 cgatcacgtc cgtggtcggt gggatgccct cgacgtcgcg tgcatccgcc gatgcgtcat
    20701 cgctttccgt gaccctcggc tcgtccacca ccccttcgcc ggtcgacgtg atgccctcgg
    20761 ttcccttggc ggtgatcgcc tcccaaggag ctgccgtgga tgccgcactc gcgcgatcgg
    20821 cccacggttt cggcggggcg ggcatcttcg cggccggcag ggccgatcct ggctcggcgg
    20881 ccgatgatcc ggacctcggt gtggcgacgg ggccggaggg tgtggtgggc gccgcggctc
    20941 cgctgggctc atcgaccgct gcgacctcgt cccgctcggc gacatccggc tcggcgtcac
    21001 cgaccttcac gaccatgggc tcttcgtcct gctcgctcgc cgcctccaca acgtccggaa
    21061 ccgcagcagt gtgcggcccg accgatgctg cctcggagct ggcgcgctgg agctcgatcc
    21121 atggcggcgg gatctcggca acctcctccc cgacggcgtc ttccgggacg gcgacgctct
    21181 cccggttcgt ggtgttggag acctggacca tccggcgctc ggcctctcgg agctggtgct
    21241 gcagttcggc cgcgcggagg cctgcctcgg ccgcgcgacg tcggtcttgg gcggcgagac
    21301 cccgctcgac ctgcgcccgc tctcgtgcct cgtcgcgctc cgccttcagg cggatcgcct
    21361 cggcgcgagc gaccgccgcc gcggcgagct tctccgtgac ggcgacgagg tcggcctcga
    21421 gctcggtgtt ccgcgaacgg agctggacga tctccgcgct ctgcgcctcc acctgggcgg
    21481 cggccaaggc caacgctcgc tcgctcttcg cggcgacggc ttccgcctgc cggcgcgctt
    21541 cctcggcggt cgacacacgt gcggagatcg cgctgagttc cgagatctcg gcctcgtagc
    21601 ggaagatctg tccgcggtcc cggaccgcct tcgccgtgag ttcgtgctcg agatctcggg
    21661 ccttgccgag catctcctcg agcgcctcga tccgctcctg cgcctgacgg atccgttcga
    21721 tcgcctcgcg tcccttgtcc tggtcctgcg gagcgaggac ctccaacggg ctcctgagct
    21781 cggcgaggcg cgcgacgagc cggtccgtcg ggcgatactg atcgggagcg tcggcgcttg
    21841 cgtcaggcct ctctgagggg ttgcgctcgt ccatgatccc ccgatgctag gtcccccgac
    21901 tggatggcgc agccgtcgaa tgggtgaaag gaacacccct cggatggact aggtcgaacg
    21961 ccacatcggc ccagatggcg agcgtcccta cgcggcgacg atgcggatcg gcatacgctt
    22021 gatgccgttc acgaagtcgg accggatccg ctccaccggt ccggcgagct cgatcgactt
    22081 caaccgcggc agcagctctt ggaacaggat cttcgtctcc agccgcgcga ggtgcgcgcc
    22141 caggcagaag tgcggcccgc ccggtccgaa cgtcacatgg tcgttcggtt tccgcgtgac
    22201 gtcgaacgtg tacggctcgg ggaagacctc ctcatcgcgg ttggcgctcg agtaccaggt
    22261 cacgaccttg tcgccgcgct tgatctccgc ccctcggagc tccaggtcgc gcgtggcggt
    22321 gcggcggaag tggtggaccg gggtggccca tcggaggatc tcctcggtgg cgcgcgggat
    22381 gcggtcgggt tcctcctgca gcagcttcag ctgctccggg tgctccatca gcgcgagcat
    22441 gccgtggctg atcgtgtgcc gcgtggtctc gttgcccgcg atcatcagca cgccgaagta
    22501 gttgtggaac tcctgctcgt tcagcactcc ctcggactga gcgaccgtga gcgcgttgat
    22561 cacgtcatcc tgcgggtcgg cgaggcgcag gtcccgctgc cggtccgcgt agtcgaacac
    22621 ctcgtaggcc gccggggagc ggaaggggag cagccggtag gcctcggtgt catcccggtc
    22681 gatgacggcg cgggataggt ccgggtcctg gttcgcgatc atctggtctc cccaggagat
    22741 cagctgcgga gcatcctcct ggggcacggt gaagatcgag cagaggaacc ggatcggcag
    22801 gtcgcgactg atctcgggca cgaagtcgaa ctcacccatc gggagtgccc gatccaagac
    22861 gtcgcgggcc acgtcgcgga tccagtcctc gtacttgccg accccccgcg ccgagaaccg
    22921 cttcgagcag atcgcgcgga gctccgtgtg ccggggagga tcggtctcga gcatcgactt
    22981 ccggatctgg agctgctcct cgtcgagctc ctccagggag acggcgccca gctcggagga
    23041 gtaggaccgc cagtccatgt ggatcgcggt gaggtcgtcg tagcgcgtca ccgcccagaa
    23101 gcccttgttg ggcgagcgct ccggatgcca gaacaccggg tgctcgatgc ggaggtggtc
    23161 gaaccactcg taggggacct catcgacgaa gctgtccggg ttcgcgaggt cgatctcggg
    23221 gtgctccgcg gccatcgccg aatcatacgg ggacaccgag aagccgccgg ccggccagtc
    23281 ggactccgcg aggcgcttca ggttccgcag gatgtcaact ggtcgctcgg acgccggggt
    23341 acgcgacgta gaagatcagc ggatccagtt ggtgacccgt gtcgtgagac gggtgtcgcc
    23401 gccctcaggc gtgagcgtgt agttcgttca agtaccaggg cgtgggaccc taggagtcct
    23461 agaagaaggg aaggtagtag tcgagctccc atgacgtgac ggcggtgtcg gaggtctcgc
    23521 cggacgcgac gtaccgatcc cactccgcct gcttcagcgc cacgaacgac tccacgagcc
    23581 agtcgcccat ctccgcggcg agcttggcgt ccttccggag ctcctcgagc gcctcctcga
    23641 gcgtgttggg cacgcccacg tccgtgttgg ggtcggcccc gggctcctgc gcggggccga
    23701 gctccagctc ttcggtcacc cccagccgcg cggcgtggag cagagccgcg cccaccaggt
    23761 atggattggc cgcggcatcg gccatgcggt gctcgatcct cgacccctct ccgcgggcgg
    23821 gcgggacgcg gacgcccacg gtccggtcgt cgaatcccca gttcgccagg tagccgttca
    23881 gcatgtccgg cttcaagcgc ttgtaggcgt tcgtggtcgg cgcacagatc gcggagatgc
    23941 cgcgatgatg ggcgaggaga ccggcgatcg actgcctggc cagatccgac aggccatcgg
    24001 gatcgtccga gtcgtggaac gcgttggaac cgtcccgccg ccggaacgag aggttcacgt
    24061 gcatcccact gccgccgcga tcgttgaacg gcctgccgag gaacgtcgcg tgacggccgt
    24121 gtcgtgcggc gatctcgcgg acgagcacgc ggaacaggaa cgcctcatcc gctcccgcga
    24181 tcgcgtcgcg atagcgccag ttgacctcga acgcagcgct gtcgaactcg ctgcaccagc
    24241 tctccacgtg gaagcccgac gccagcgagg cggtcaccat gtcgtgcacg gcgccgctcg
    24301 ggtcgacgga catcccggtg ccgtagacgc gatgggacgg gagatgcgcg ggctcccacg
    24361 agccgtcgtc cgcccgctcg cacaggtaga actcgagctc gaacgcgatc tggggctcca
    24421 gatccaactc gcgccaggcc cgcaccgccc gcttcagcac gtgccgaggg tcgacctcga
    24481 gagggacgcc gtgctgcgtg acgtcggcga taccgaccac ggtgtcgtcc tcccaccccg
    24541 gccggagggt gtcccgatcc atcgtcgcct cgacgtcggg gaggccgacg tcgaagtgct
    24601 ggcggcggac cggcaggatc tccttgtccg tcgtgagcgg atacagcccg atgcagaacg
    24661 ccgcgtgacc ggcttcggcg acgtcgccga acagatactt cccgcgctcg aggcccagga
    24721 tgtccgagat gagcaaccgc agacgtctgc cgctcaggtc catgtcgcgc gccttcctcg
    24781 tgtggcaacc aggaccgtat ccgatgacgg cgcgatccgc ctgaggatca gccgcgcttc
    24841 ggagccctga ccccccggaa ctcccagtcg ccgccgaacg cggtcgagac cacctcctcg
    24901 gatccgctcg gctgagcgcc cacgtcctcg cggatcggca cctcaccctc gacgatgaag
    24961 ttgcggagga ttccgaggcc ctccacctcg acctcgacga cgtcgcccgg ttggacgggg
    25021 cgcgagctcg cgggggtgcc cgacaggagc acgtcgttgg gcaggagcgt gatcgtgcgc
    25081 gcgatgtcgg cgacgaggta gcgcatgtcc cagatcatct cgtcggttga gccgtcctgc
    25141 accacctccc cgttcacgcg cgtctggatg cccttgctgt ggaagtccca gtccgtcacg
    25201 aggccgggac cgagcgggca gagcgtgtcg gagcccttca cgcggagcat cgagccggcg
    25261 tcggtgtccc ggaagtcgtg caggccgaag tcgttcgcga tggtgtagcc ggcgatgtac
    25321 tcgtccgcct cttcgggagc gatgtgcgtg caacgctcgc ccatcacgat cgcgatctcg
    25381 ccctcgtagt tcaggtaccg gcaccgcgcc gggcgcacca cctcgccgcc gtgcgcgttc
    25441 aacgacgtga tgggtttgtg gaagaacgtg ggtgccggcc ccagctcggc gaggaactcc
    25501 gcccgccggc tctcgtagtt gaggtggacg cacacgatct tcgagggttg gatcggcggg
    25561 aggtgggtcg gctcgtccat cgggatccgg cggccatcgg gagcgacgag ttcgtctcct
    25621 gctcgggtga cggtggtcgc cacgccgtcc aggaggatcc gtcgcgtctc catccctcga
    25681 tagcctagag gtcgtgcgct gggtcgtcgg aaccgcggag gggctcgtcg gaagcgaggg
    25741 cgagctctgg ctcgtacatc gagccatcac ctcgctggcc tgggacggcc acggctggct
    25801 cgtgctggtc gacggccgcg aggtgctgcg ggtccacgga gacgaggtcg aggcggtcgg
    25861 tgagatcggc gacgaagccg gacgctgtct cctgccgacc gccgacgggg tgctgctcgg
    25921 cacggcgcat gcgcgtctcg gcctgcttcg cgacggtgcc gtcacgatgc tccgctcgtt
    25981 cgatgaggcg gaaggacggg acgagtggta cacgccgtgg ggcgggcctc cggacacgag
    26041 gtcgctctcc gcgtcggccc acggcgccct cttcgccaat gtccacgtcg gcgggatcct
    26101 gcatgcgcag gtccccgacg gggtctggtc accgacgatc gagatcgacg ccgacgttca
    26161 tcaggtcctc gcggatcccc tcgacgactc ccatgtggtc gccgcgacgg cgcgcgggct
    26221 cgccgagacc cgcgatgcgg gggccacctg gcacttcacc gcggagggac tccacgctcc
    26281 gtacgcccgc gccgtcgctc tggacggaga ccgactcttc ctgagcgcct ccacgggtcc
    26341 ccgtggcggc cgggcagcgc tctaccggcg ggagccggga gcggatgtgt tcgagcggtg
    26401 cgcggaaacc ctcccggact ggttccccgg gaacatcgac agctactgcc tcgccgcggg
    26461 tgagggcgcg atcgcgttcg ggaccgagga cggccgggtg ttcgtctccg gcgacggtgg
    26521 agggtcttgg cgcgaggtcc tcggcgagaa cgcgcgcgtg acgtgtgtcg gcgtggccgc
    26581 ctcggaggga cccggcacgt ccacgtagac tccgcatccg tggccgagct gaaggggttc
    26641 ctcccgccgc gatcgcccga gggcagggcg tcgctcgtcc cggcaccgcc gtggcactac
    26701 tcaggcgacg tgatgacgat cgagttccgc acggatcccg gtcgcgtcgc ggcgctcctt
    26761 ccggatggtg tcgagcccgc cgaggaggac cccggcgccg tcgcgatcgt ctgggccgac
    26821 tggcaatcgt gcagcgactc cggcgaggaa ctgctcgacc ccgtgcgctc gcaatacaag
    26881 gaatgcttcg tggtggtccg ctgccgatac gagggagaga cgtactcgcg gtgcgtctac
    26941 atctgggtcg acaaggactt cgcgctcgct cggggttggt accagggcta cccgaagaag
    27001 ctcggctcga tctggatgac gcgcccggtc tcggtcggcc gggctgggcc tcggctcggc
    27061 gccggcgcgg tgttcggcgg gacgctccag gcgaacgacc gacgcctcgc tgaggctcgc
    27121 ctcacgctca cgggtggctc ggagtccgca gggttcgtca acgccctccc gatgctgcac
    27181 tcgcgctgga tgccctcgat cgagaaggac aacggcgact cgctcgcgga gctcgtcacc
    27241 atgcgctctc gcgacgtcga cctcggtccg gcgttcaccg gtgacgcgga catccggctg
    27301 ttcgacgcgc cgggtgagga gctcgcggga ctcgagcctt ccgagatgat cgcgggctac
    27361 tggcgtcagg tcggcgcgac gttcgacggc gggtccaggc tcgcgtagtc atgcgtctgg
    27421 ctcgattccg atacggtgac cgcatcgcta ccggcgcgat ggatcgcgga tccgaggaga
    27481 tccgggtcct ccgggggacg ttcttcgagg atccacttcc gacgggcgag accgtcgcgc
    27541 tgagcgacgt ccacctcttg gcccccgtcc tcccgtcgaa gctggtatgc gtcggcagga
    27601 acttcgtggc ccacgcggag gagtggggac tctcggtccc cgaagagccg ttgctgttcc
    27661 tgaagccgtc gacggcggtg atcggccccc gcgatccgat ccagctgctc ccgatcagcc
    27721 ggcggatcga ctacgaggga gagctcggcg tggtgatcgg tcggctggcg cgcggcgtgc
    27781 gtgccgagga ggcgttcaag tacatcctcg gctacacgtg tggcaacgac gtgacgctcc
    27841 gcgacctcca gaagaccgac gatcagtggg cgagggcgaa ggggttcgac gggtcggcgc
    27901 cgctcggacc gtggatcgag acggacctgg acccgaacga cgcgatcgtg aggacccggc
    27961 tgaacggcga gatccgtcag caggcgtcca cttccgacat ggtcttcggc gtcgcgacgc
    28021 tgatcgagta cacgaccacg ttcatgacgc tgctccccgg ggacgtcctg ctggccggca
    28081 ccccggaagg ggtcggcaag ctcgccgacg gagacatcgt ggaggtcgag gtcgaaggcg
    28141 tgggcatcct ccggaacgag gtccgcgggt gaaggtccgt tgccgattcg cgccggcccc
    28201 gtcgggctcg atccacgtcg ggaacgctcg cagcgcgctg ttctcgtggc tctacgcgcg
    28261 tcacgagggc gggacgttcg tcctccgcct cgaggacacg gatgcgagcc gtgtgacgga
    28321 agaggcctcc cagggcgtcg tcgaggacct ccgttggctc ggcctggcat gggacgaggg
    28381 cccggatgtc ggcggcccgt acgggccgta ccggcagtcc caacgatcgg agatctaccg
    28441 tgagatggcc gaccggctgc tcgaggaagg ccacgcctac cggtgctggt gcacggccga
    28501 ggagctcgat gagcgtcgga aggcagcgct cgctcgaggc gagccgcccg gctacgacgg
    28561 acggtgccgc cagcgcccgg cgggggagat cgccgcgttc gaggcggagg ggagaccgtt
    28621 cgccatccgt ttcctgatgc cggagcgggg atgggccgtc gaggacctcg tgaagggcga
    28681 ggttcgatgg ggatcgaacg acctccgaga cttcgtgatc gtccgttccg acggatcgcc
    28741 cgtgttcctg ctggccgtcg ccgtcgacga cctactgatg gagatcaccc acgtgatccg
    28801 cggcgacgac ttgctcgcga gcgcaccccg taacgcggcg gtgatcgagg cactgggggg
    28861 gacgccgccc gcctacgcgc acctcccgca ggttctcgga ccggactcga agccactgtc
    28921 gaagcgacac ggcgacacga gcgtcgccgc cttccgcgag cacggattcc tccccgaggc
    28981 gatggtcaac tacctagcgc tgctcggctg ggccaaggac gaccacacga cgctcctgac
    29041 ccgcgacgag ctcgtcgagg cgttcgatct ctcccacgtc tcccacaacc cggccgtgtt
    29101 cgacgtccag aagctcgagt ggatgaacaa ccattacatc cagtcactcg acgaggagga
    29161 gctcgccgca cgatgcgtgc acttcctcac cgaagagggg ctctccctcg atccggacct
    29221 gctccggcgc gcgatgccta tcgtccgcga acggatgaag accctcgcgg aggcgccgat
    29281 cctgcttcgg ttcctcttca cggacgacgt ccggctcaac gagaaggcga cggacctgat
    29341 cgcgaaagcc ccggaggggt acctcaagga ggcggccgaa gcgctcgatg ccctggagcg
    29401 atgggacagc gaacacatcc aggcggcgct ggacacgttg gcggccgcgg ccggcctgag
    29461 ccgaaccaag gggtggcagc cggtgcgcgc cgcggtgacg gggtcgaacg tgtcgccgcc
    29521 cctgccggag tcgctcgagc tgctcggcag ggaacggaca ctcgcccgtc tgcgcccggc
    29581 cgcccgcgga tgacgcggcg gatcctcttc gtggcggtag ccgtcgtaca cgccgttggt
    29641 ctcacgcccg ccgtcggcct tacgcccacc cagctggcgt ccccgacggc ggcggtcgtc
    29701 gccgccgagc cgctcgaacc tgacgtcgcc tcactgcggc cgcggatcgt gtggaagccg
    29761 atcccgtacg gcgctcgccg tcgccgtcag atggccgcct actcgcggcg tcactacggg
    29821 tcgtgggggt ggaagctcga ggcacccgtc gcgatcgtcg agcactacac caccggcacg
    29881 acgtggcagg gagcatggaa cacgttcgcc tcaaactcga agcacctcgg ggagtacccg
    29941 ggcacgtgca cgcatttcat cgtgcacacc gacggcacga tctaccagct ggcatcgctc
    30001 ggcatccgtt gtcgccatac gatcgggatg aaccaactct cgatcgggat cgagcacgtg
    30061 ggacgctccg acgcggaggt gctgaggaac ggctcgcaga tgcggtcttc gctccggctg
    30121 acgctgtggc tcgtagcgag gttcggcatc gagatccgga acgtgatcgg gcacggcgag
    30181 agtctccaga gcccacttcg ctacgagcgg tacgcctcgt ggagatgcat gacgcactcc
    30241 gacatgtcgc ggtccgcgat gatcacgtat cggggacggc tgcgcgatcg ggctcgcgcc
    30301 gcggacgtgc cgatcgggcc ggcgcccgcc tgggtcgata tcggctgctg atcgctcgag
    30361 acgatttccc ccgcgcacca tcggggtcta ggatgtcgga gcaggaggcg cccgtgtcga
    30421 aggtcttgaa cctgtccgag cgccaggacg ccgagggatc ctggcacatg gtcctgaagg
    30481 acgtctgcgg ctggtgcggc tgcgggtacc tcgtcgccga ggacgaccac gacctcgtat
    30541 gggagcccgg tcggcatcac gacaagcact gctccgacga cgcgtgtgag tgtcataccc
    30601 gcgcacgggt gggcaagcgg cacgacgagt ggtgagcaga gcacggacgc tagctcgagc
    30661 tacctcgagc tagcccttct gagccacacg ccggtccggt gaggtctcga ggatcgtcaa
    30721 ccgacggcag atgtgatcct ccacgtgggc catctaaggt cacccaacga ctcagatgac
    30781 ggatcgatga cggatgacgg aggacgcgat ggtgggcgag gacggatcga tgacgatcga
    30841 gagcgcagcc gagctgatga acgtggaggt acggtcgatc cgccagtggt cggcgatcgg
    30901 atcgctccgg atcgacgtgg agctggtgca cctcggacag gtgaaagccc tcgcgcgctc
    30961 cccgatcgtg atcggcgatc gcaaggagaa ccagcgaggc gggttgcggg cgctccttcg
    31021 cgaggcgacg ccggtcgatg cgcagagcgt cgcggggctc caagcgctgg ctcgcgagcg
    31081 agggtgaggg agccggcggc cctgccaagg cggaaaggtc cggttcgtcc tgtgcggatg
    31141 ggttcccgta ggcgtgaagc gtctcgcgga cctgatcccg aaaccagctc cgcgtagcac
    31201 ggctccaggc tctcgaagtg aaacggcccc cgcctcggac cgttctctcg tggtgggtcg
    31261 tcaccgctcg cggcggtacc ctccgggcga acagccgtcg gtcaggagca gaagctggag
    31321 cctcgcggac cgagatctgt cgccgtcgac cggacgacga tcatcgcggt ggccgccctc
    31381 gtcgcggcat ccgccgtcgc ctgggtggcg ctcgtgacct acgagccccc gatgggattc
    31441 ctgggcttca ccgctgggtg gtcgctgatg atggcggcga tgatgctgcc ctctatcgcg
    31501 cccctctggc tcctctcccg cggatcgcgc gtggggatgg ccgtgggcta cctggtggta
    31561 tggagcgccg tcggagtagt gccctacgtg gccatggagc gcgcgatgga catgaccgtg
    31621 ccggtcgtcc tgggcctggc cggggtctac gagctcagcc cgctgaagtc ggcctgcctc
    31681 cgccgatgcc gaaacccggc ggcgttcctg atggagcatt accggagcgg ggcggtgcgg
    31741 ctcggcgttg cacacggcat ctggtgtctc ggctgctgtg tcggcctgat ggccgtcctc
    31801 gtcctcgccg cgtcgatgca gctcgtgtgg gcgatcgtga tcgcggccgc cgtgttcgtc
    31861 cagaaggtcc ttccacgcgg tgaggtgttc gcccggttga tcgggatcgg cttgctcgtg
    31921 gcggcggcgg ccgtcgcgct gacgtgaaag gggagcgatg ggtgacgtgt ggtcgatgaa
    31981 ggggacggtc gtcgtcgcgt gcaactgcga ctacgggtgc ccgtgcaact tccagtccct
    32041 ccccacgatg ggcaagtgcg agggcggatg gacctggcac gtcgacgagg gtgcctacgg
    32101 ggacgttcgt ctcgacggca tcacgttcac gctcacggtg aactggcccg ggccgatcca
    32161 cgaagggaac ggggtcggtc tcctgctcgt ggacgaacgc gcggacgagg cgcagcggaa
    32221 cgcgatccac accctcgcga aaggtgaggt cggaggaccc tggggcgtcc tcggctggac
    32281 ctggccgacg ctcgaaggtc cggaggcggt cccgtacgat ctccatctcg acggcatccg
    32341 atcgagcgtc cgggccggcg acgtctacga gctcgattcc gagccgatcc ggaacccggt
    32401 gaccggcgtg gagatcaccc cgggtgtgat gctgccccag gggacgatct tcaaggaagg
    32461 tctcttcggc tcgtccaagc ggctgcgggt cgatcggctc gacatggact acgcgaacaa
    32521 gtacacggcc gtcgcggcct tcgactaccg aggtccctag atcgtcggag cgtcaccctt
    32581 cggaccggac ggatcccgga gcgccgcctc gagctcgtcg aggtgctccc cccggtgcga
    32641 cgcgcgcagc gcgtagagcg ggctgtcagg atctcgaggc cagagacgat ccacggggag
    32701 ggtcgccacg cgcgcgtcgg tttcctcggc gatctcgagg gcgagccgag cggcctcgcg
    32761 cggcgggatc gcgtggatca gtgcgcggct ggcgtcattg atccagtcga cgtcctcggg
    32821 ctccgtgtcg gagatcgtga acggctctcc gcgctcgagc ttgtcggcga gcgacaggac
    32881 ccgggcgtcc cagaacgcta tgtggccgag gacgccggcg acggtccagt gcgggttcac
    32941 cgagaggcgc agttcgtctt cgctcacccg atccacgatg gcgcgcatcc gctcgcgctc
    33001 gcgcgtgttc tcctcgatgt aggatcgatc ctccgacatg gctccacgac gatagcgcgc
    33061 cggagcgagg tcggttgatc cagaccctgg tggttcctaa gggatcatct gaaccgtgtc
    33121 tttaccgtta gggtgttctc gggccgatcc gctacctgcg gcgatatccg aatctgatgc
    33181 ttgacctgcg gatcccttgc tccacacctc ccctcttctc ttgacgttcc cggtcgttcg
    33241 cgggacggac gcgggatggt ggcggactcg atcgtgtggt tccgtacccg catcgtgacc
    33301 tgacccgcat ggaccttcga cccgcgcggg caaggacctc cggcacgtga agggcggctg
    33361 ctcgctcgcg aagctatgcc caaccggggg taccgcagtt ccccaagaaa cggggcacgc
    33421 gacgaagagg cagtcgcatg aaagacaacc acgagagcat cgtgaagcgg ctccgccgga
    33481 tcgaggggca ggtctcgggg atccgcaaga tgcaggagag cggccgctac tgcgtccacg
    33541 tcctcgacca gatctccgcc gctcgggccg gtctcgaggc gaccgcgttg ttgatcctcg
    33601 aggaccacgt gaacgggtgc gtccgtgagg cactcgatga tgggaagggc gaggagaagg
    33661 ctgcggagct gctccaagcc gtcggccggt tcgcgaggag tgtgtgacgt gtcgccgagg
    33721 gacatggcgc tgctgacggt ccccgagccc gggttctgcg gaacgttcct cgttgccgcg
    33781 cgcattgccc acgtcgtcgg gcacaccaag ccgcaacaga ccgagcgttc cagggagacg
    33841 tgaaccccat gggctctccg accgcccctg gtggtggacg atgagcggaa gatctgcgac
    33901 ctcgtccgct cctacctcga gctggagggc gaccgacgcc aagacgatcc tcgagatgcg
    33961 gttcgcgaga ggcgagatct ctcgggagga gttcgaggag cggaagcgcg tgctcgagtc
    34021 ccgggccgca tgacagcgcg gtagcacagg gaacacgacg gggccgggtg gagtgtcggc
    34081 cggtccccgt tgacgacgtc cgcgcaccgt ggaggatcgg cgtccgcttg tgtgcaagtc
    34141 ggtgcaacgg tgcctggggg atgccgcccg atcgccctca caagtgcggg accttcgacc
    34201 ctcctgtcgg cccgccggcg ttcatagcct taccccggta gggcatagga ggacgcgatg
    34261 agtccgagac tcgtgaacgc agcggttccg gtgggtcggc ggaacctgct cgccgaccgt
    34321 cgtcgcctgc tgatcagcgt cggcggggtg ggggcggcgt tggcgctcat cctgttcctc
    34381 cagggacttt ggctcggatt ccagcggcag atcaccgcct acgaggacaa cgtcggcgcc
    34441 gacctgttca tcgccgagcc gggcacacgc tcgttcctcg gcgagtcttc gaccgtctcg
    34501 ctggagtcgg tggcggagat cgaagcgatg ctcgacgtcg atcgggccga cgcgatcgtg
    34561 tcgcgaggcg ccgtcctgga cctccatggt cgcaagcagt tcgcgtctgt gatcggatca
    34621 cgccccggtg gcctcggcgg accctgggcc ctcgcggagg ggcgggtctc gcgggcactc
    34681 gacgaagcgg tcatcgatga gacgcttgcc agccagcaca gcctcacgct cggggacacg
    34741 atcgacatcc tcggcgctcc gttccggatc gtcggtctgt cgaccgggac gcggtcgtgg
    34801 atgttcgggt tcgtcttcgt gacggtggac gcggcccaaa ggatcacccg ggcgcccggc
    34861 tccgtgagct tcgtcctcgt tacgaccgag catccgacgt ccgtggccga gggcatcagg
    34921 gacgagctgg ggctgtcgac gctgtctccc gaggagctcc gatcgaacga ccgcgccctg
    34981 ttggcgaagt cgatcgccgg accgatcgag ctcatggtcg tcatcgcgtt cgcggcaggc
    35041 acgctcatcg tcgccctcac cgtgtactcg caggtgatcg agcggatccg cgagtacggc
    35101 atcgccaagg cgatgggggc gagggccgga cgattgttcc gtatcgtgct cggccagacg
    35161 atgatcgtgg cgctccttgg agccgccctc gcgttcccga tgttcctgct gggggcgaag
    35221 ctcgtgaacg tgctccggcc ccagttccgg gccgacatgt ccgctccggt ttcgctcgtc
    35281 gtcgtcggcg cggccacgct catggcgctt ctggccgcgc tgctcccgac acgtcgggtg
    35341 tcgcgactgg atcccgcgtc ggtctaccgg gggtgaggat gatgcgacgg gtcgcgatcg
    35401 gccgcaagaa cctcttcgcc gaccgccggc gagcggcgct cggtgtggcg ggtctcgcag
    35461 ccgccctcct gctcgtactc gccctcgatg ggatcttcgc cggggcgatg cggggcgtga
    35521 ccctatacat cgacacgtcg gccgccgacg tgttcgtcgc acagcggggc gtgaggaaca
    35581 tgcacatgac ggcatcgtcg gttccgctgc gtgccctgga cgaggtccgc gccatccccg
    35641 gcgtcgtgtg ggctcagccg atcgcactga cgagcgattc gctcgaggtc ggagcccagc
    35701 gccagctggc caacgtgatc ggctacgagg tgggggaacc gggaggcccc accgagttcg
    35761 aacgaggtcg gccgcccgaa gccggtgaga tcgtcctcga cgatcgcgcg gccgagctgc
    35821 tgggcgtggg gctcggcgac gacgtggtga cggtcggcct cagctggcgc gtgtcgggga
    35881 tctccagctc gatgaccaac atcgtgaact cggtggccta cgtgacgttc caggacctcg
    35941 tctctgctcg aggcatgaac gagacggcga gctacctgct ggtgaaggtc gcagggtcaa
    36001 cagatggggt cgcctcgcgg atcgaggatt ccaccgggct cggcgccctg actcgggcag
    36061 ccttctccgc cgaggagtcg caggcggtac gcgacatgag cacggagctc ctcgccatca
    36121 tgacgctctc cgcgttcatc atcgccctgg tcgtcgtcgg cctcaccctg tacgccggca
    36181 cgcttgcccg cctgcgggag gtgggggtca tgaaggcgct gggggcgggg gccggcccgc
    36241 tgggagcggc ggtgctgtcc caggcgatct ggacggtggc ggccgcgacg gcgacggcga
    36301 tcttgctggc cctcggcctc gcctgggccc tcgccggcat cggcagcacg atctcggtgg
    36361 tgatcgagcc ttcgtcggtg atccgcgcgg ccgccggcgc gctcgtgatc ggggggctgg
    36421 gcgcggtctc gcccttgatc aaggtgctgc gcgtagatcc ctcatcggtg ttccggaggt
    36481 agtcatggac gacatcgcga tcagggcaga gggattggag aaggtctacg gggagggcga
    36541 cgtcgaagtg cgagccgtcc gcgggatcga tctcgaggtc cgccggggcg aggtgatcct
    36601 gatcatgggt ccctctggta gcgggaagac gacgctgctg tcgatgctcg gggccatgct
    36661 ccgtccgagc tccgggcgga tcgtggtcga cggagaggat ctcgccggtc tctccgagcg
    36721 gcatctcccg gccttccgag cccacaggtt cgggttcgtg ttccaggact tcaatctgct
    36781 cgcggcactc accgcacagg aaaacgtgga gttcgctcag aacctcgccg gcgtcacagg
    36841 acggcaggca cgggaccgag ccgaggggct cctccgggat ttcggcctgg gcgagcgctt
    36901 gcggttccgc cccgagaagc tctcgggcgg agagaagcag cgcgtggcga tcgcccgggc
    36961 cctggtgaat gagccggcgg tggtcctggc cgatgagccg accgcgaacc tcgactcgaa
    37021 gatcggacac gagatcgccc ggctgctccg gagcgcggcc agcgagcatg accggagcgt
    37081 ggtcatcgtg agccacgatg ccaggctgaa ggacatcgcc gaccgggtcc tgtggctcga
    37141 ggacggggag ttccgatcga tgagcgagat ggccaccgat ccggtctgcg ggatggcggt
    37201 ggagcgcgag ggtgccccgc actaccggtg ggatgggacg acgtcgttct tctgctcgcc
    37261 ggcgtgccgg gacgagttcg ccacggagcc cgggcgcttc gccggcgccg tcagggcgga
    37321 ggcctgagtc atggatgcag cggtggggct cgtgaaggcc tacctggagc tcagcggcta
    37381 cttcgtcctt gccgagctgc cggtgaagga ggacgggcag agcgggttcc gagacgtcac
    37441 cgaccccggg ggaggcccac ctgaacccgc gctccgcagg tccgacacga tcgcgttcgc
    37501 cctgaggcgc gtggcgtgct gcccggagga tcgggtggag gcggaagtcg agatgatcgc
    37561 ccgacgtgac gccgggaacc gaccgtgccc ccggggtgac gcaaccctcg ccctagcgcg
    37621 agaccatccg gccgacagcg cccatcagct cctcggcctg ctcatcgggg tcgttgggac
    37681 ccccttggcc gccgacgagg cagtgtcgga cgtgaccgtc cagcagaccg agcgcgacct
    37741 tgtcgagggc cgcccgggcc gcgttgatct gggtcaccac gtcgatgcag tagcggtcct
    37801 cctcgaccat ccgggcaacg ccccggacct ggccttcgat cctggccagc cgtgcaagca
    37861 gctggtcctt cgtcgccgtg tatccacggg tctgcgtcgg catgatctcc tccttgtgcc
    37921 cgatgatcgt gttgacgtat aatacccctc cagggtatag ggaaatcaag ggttggatcc
    37981 acctgtcggt tggaagcggg ccgcgacgtc cgagtcattg gatcggaggg cgcggccggg
    38041 gagcgtgagg gagcatgacg ggaaccgaga tcttggtgac tgggagcggt gtcgccctca
    38101 tcggcgcgct cgcctacttc ttcttcgggc cgaagcatgc cggccgggca gaggtccgag
    38161 gcggcgttca ggaagtagag atcactgtga agggcggcta ctcgcccagc gtgatcaagg
    38221 tgcagcaggg cgtgccgctg cggctggtct tcgatcgcca ggagcggggc gagtgcacct
    38281 ccgaggtggt cttccccgac ttccggatcc gccgcggact cccggccttc gcccgaacga
    38341 cggtcgacct gacacccgag cggagcggcg agttcgggtt cgcctgcggg atgaacatgg
    38401 tgcacggcac gctcgtcgtc gagccggcgg agggcgacgt cgctggggca ccggaacgcg
    38461 ttgatacgtc ggtctcgggg gcgctcgcgg aggcaaaggc cgtcgacggc caccggcatc
    38521 aggagacgag gacggtctcg atggagccgg gtggcgaggc ggccgggcgt gtcgccctcg
    38581 cgctcgagcg cggagggggc gtcgcctgcg ctgcgtgcct ccgaacgatc gagtccgaac
    38641 tggagagctt gcccggcgtg cggagcgtgc agaccgacgt cgtcaacgag cgagcctcgg
    38701 tcgagttcga tccgtcggcg gtcccggtgg aagacatgcg cgatgccgtg gcctcgatcg
    38761 gataccgggt ccagcagcca ccccgagcgg cgtcggacgg gacggaggac gtcgaagccg
    38821 ccgcacgccg ggcggaggtc cgcgacctca cgtggcgggt ggttgtcggc gctgcgctga
    38881 cggcgccggt gctgatcgcc gtgatggcga gcgagatctt cgacgcggcg tgggttcccg
    38941 agttcctgat gaaccggtgg atccagctcg ccttgaccat cccggtcttc ctctacacgg
    39001 gatggccgat ccacaccgtt gggtggcgga cgctcctgca ccgcaccgcg gagatgaata
    39061 cgctcatcac ggtcgggacg acggccgcgt tcgcctacag cgtcctcgtc acggtcgcgc
    39121 ccgggctgtt ccccgcagag gtccgcgagg t
//
LOCUS       DASUYD010000040         4290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1123532, whole genome shotgun sequence.
ACCESSION   DASUYD010000040 DASUYD010000000
VERSION     DASUYD010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123532"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1900)
                     /locus_tag="VF195_02260"
     CDS             complement(<1..1900)
                     /locus_tag="VF195_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6579681.1"
                     /translation="MSAGISPATTTFIRPDGREKVTGVGRYTADLTTTGMAHASFRYA
                     DHPHARVLRIDTTRARALPGVVAVVTQEDLPDVRFGGLVQDRTLFAKDVVRFEGEIVA
                     GVAALTEAIAAEAARLIEVEYEPLPVITDFVAAMEPDVPLVHAAWASYERDENMVSNG
                     NTLGYHTIVKGDADAALAQADVVVGSWYESDPSQGVPIEPRAVLAEWRGDEVTIWSST
                     QVPYAARDGVAQTLRIPEANVRVVVPLLGGGFGAKCDLHFEAQVAALARAARRPVRLV
                     FSREEEFRAIAQRREGIVMEFETGATRDERLVARRARLILDKGAYCGEGGFLGQMAAM
                     HACGPYQIDNVFVESFLNYTNQQPSGSVRAPTAPQVCWGLEQHMDEVAEGLGIDPVEL
                     RRRTLIEKGAEGPTRQIFDEIGVKQTLERAVEMIGYERELPEDEAIGVAVGWWPSSPA
                     ASGAYIHLNGDGSGTIVTGAQENGSGAVMAMPTFVAEELGMDPENFTLLYQDTAAANW
                     DMGSCGSQTTFNSGRAILAAAADVRDQLLDAAASELEADRGDLELVEGHVRVKGSPGR
                     SVSIADLAGAGTIHGKGSGEVPDTPTGDTGGCVGRIGNETFAAPQLITQAAHVKVDRE
                     TGVVRVLKV"
     gene            2010..2477
                     /locus_tag="VF195_02265"
     CDS             2010..2477
                     /locus_tag="VF195_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6579682.1"
                     /translation="MDREHRAELLARYRAGVDEVDAALAGAEGADLDVVPSDGGWSAR
                     MVIHHLADSESNSYLRVRKLLAEDDPMIQGYDQERFAERLHYERPAETSLAVFRAVRA
                     STAELLDGLGDEDWSRAGTHSESGAYSMEDWLEIYAAHAFDHADQIRRARGAA"
     gene            complement(2474..2929)
                     /locus_tag="VF195_02270"
     CDS             complement(2474..2929)
                     /locus_tag="VF195_02270"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6579683.1"
                     /translation="MSQPSQKQELLAEEDRLWSELHGLVDALPADKVEEPGYFVEGWS
                     VKDLVAHIGSWLAEAGAVLERIRFGTYRPEELDIDATNRTFYDDMHDVAFADVRAQGI
                     AARNQMLRAWRSLPEESPEADRWISKAGPEHYAQHLPRLREWVRELGAS"
     gene            2986..3132
                     /locus_tag="VF195_02275"
     CDS             2986..3132
                     /locus_tag="VF195_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6579684.1"
                     /translation="MERTTRETLLRIFEETETIAVVGASTTEGKPGHDIPLYLQSQGF
                     GSSR"
     gene            3129..3425
                     /locus_tag="VF195_02280"
     CDS             3129..3425
                     /locus_tag="VF195_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6579685.1"
                     /translation="MNPRGGRILGEPSFASLRDVGVPVDVVDVFRPREEAGTIARDAI
                     AIGAKTLWFQPGTHTDEAVAQARAAGLTVVARRCMGVIHGWLGLGPGPHVPSVA"
     gene            complement(3439..3711)
                     /locus_tag="VF195_02285"
                     /pseudo
     CDS             complement(3439..3711)
                     /locus_tag="VF195_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            3770..4237
                     /locus_tag="VF195_02290"
     CDS             3770..4237
                     /locus_tag="VF195_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006143063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6579686.1"
                     /translation="MEWSPSFYELRRGIGCAMCQQGRPEATDDGVRFYAGEVSDAYLR
                     RTNIQRGLTVVIWRGRHVAEPTELNDEEAHGYWRDLLIVGRTLEDVLRPVKTNYNVLG
                     NAVPHLHTHIVPRYADDPRPGWPFPFPEEEPPAMPEEELQRDLDALRMAIDAR"
BASE COUNT          665 a         1519 c         1436 g          670 t
ORIGIN      
        1 ccaccttcag aacgcggacc acgccggtct cgcgatcgac cttcacgtgc gcggcctgcg
       61 tgatcagctg cggagcggcg aaggtctcgt tcccgatccg ccccacgcat ccgccggtgt
      121 caccggtagg cgtatcgggg acctcacccg agcccttgcc gtggatcgtc ccggcgcccg
      181 cgaggtcggc gatcgacacg gatcgaccgg gtgagccctt gacgcggacg tggccctcga
      241 cgagctccag gtccccgcgg tcggcctcga gctcggacgc ggcggcgtcc aggagctggt
      301 cgcgcacgtc ggccgccgcg gccaagatcg cgcggccgga gttgaacgtg gtctgcgacc
      361 cgcaggagcc catgtcccag ttcgcggcgg cggtgtcctg atacaggagc gtgaagttct
      421 cgggatccat gccgagctcc tcggccacga acgtgggcat cgccatcacc gccccgctcc
      481 cgttctcctg cgccccggtc acgatcgtcc cgctgccgtc gccgttcaga tggatgtagg
      541 cgccggaagc cgcaggcgag gagggccacc agccgaccgc cacgccgatc gcctcgtcct
      601 cggggagctc gcgttcgtag ccgatcatct cgacggcgcg ctcgagcgtc tgtttgacgc
      661 cgatctcgtc gaagatctga cgggtgggac cctcggcgcc cttctcgatc agcgtccgcc
      721 tgcggagttc caccgggtcg atgccgagtc cctccgccac ctcgtccatg tgctgttcca
      781 gaccccaaca gacctggggc gcggtgggag ctcggaccga gcccgaaggc tgttggttcg
      841 tgtagttgag gaacgactcg acgaagacgt tgtcgatctg gtacgggccg cacgcgtgca
      901 tcgcggccat ctgccccaag aacccgccct cgccgcagta cgcgcccttg tccaagatga
      961 gccgcgccct gcgcgccacc aggcgctcgt cccgcgtcgc gccggtctcg aactccatca
     1021 cgatcccctc gcgacgctgc gcgatcgccc tgaactcctc ctcgcgggag aagaccaacc
     1081 tcaccgggcg gcgcgcggcg cgtgcgagcg cggcgacctg cgcctcgaaa tggagatcgc
     1141 acttggcccc gaaccccccg cccaggagcg ggacgaccac ccggacgttg gcctcgggga
     1201 tccgcagggt ctgcgcgaca ccgtcgcggg cggcataggg aacctgcgtc gatgaccaga
     1261 tcgtgacctc gtcgccccgc cattcggcga gcacggcgcg cggctcgatc ggcacgccct
     1321 gcgagggatc ggactcgtac cagctcccga cgacgacgtc ggcttgcgcc agggcggcgt
     1381 cggcatctcc cttgacgatg gtgtggtagc cgagcgtgtt gccgttcgac accatgttct
     1441 cgtcgcgctc gtacgatgcc cacgcggcgt ggaccagcgg gacatcgggc tccatcgccg
     1501 ccacgaagtc cgtgatcacc ggcagcggct cgtactccac ctcgatcagc ctcgcggcct
     1561 ccgcggcgat cgcctcggtg agcgcggcca cgcccgccac gatctcgccc tcgaaccgca
     1621 cgacgtcctt ggcgaacaac gttcggtcct gcacgagccc cccgaaccgg acgtcgggaa
     1681 ggtcttcctg cgttaccacc gcgacgaccc cgggcaacgc ccgcgccctc gtggtgtcga
     1741 tgcgaaggac gcgtgcatgc gggtggtcgg cgtagcggaa gctggcgtga gccatgccgg
     1801 tggtcgtgag gtcggccgtg tagcgcccca cacccgtcac cttctccctg ccgtcgggac
     1861 ggatgaacgt cgtcgtcgct ggcgagatgc cggcactcat caggtgccct cccttccggt
     1921 ggcgcacatc atggtccgag ccccgtgggg ccgacaaatt ggagcgggcc gccattgccc
     1981 acccgcggct ggctcgctac ggtgacctca tggaccgcga gcaccgggcc gagctgctcg
     2041 cccgctaccg ggccggcgtc gacgaggtcg acgcggcgct cgccggcgcc gaaggtgcgg
     2101 acctcgacgt ggtgccatcg gacggagggt ggtcggcaag gatggtgatc caccacctgg
     2161 ccgactccga gtcgaactcg tacctgcggg tgcgcaagct cctcgccgag gacgatccga
     2221 tgatccaggg atacgaccag gagcggttcg cggagcgtct gcactacgag cggccggccg
     2281 agacctcgct cgcggtattc cgcgccgtgc gcgcgtcgac cgccgagctc ctggatggcc
     2341 tcggcgacga ggattggagc cgcgcgggga cccacagcga gagcggcgcg tactcgatgg
     2401 aggactggct cgagatctac gcggcgcacg cgttcgacca cgccgaccag atccgtcgcg
     2461 cccgtggcgc ggcctaggac gcaccgagct cgcgcaccca ctcccggagc cgcgggagat
     2521 gctgcgcgta gtgctcggga ccggccttgc tgatccatcg atcggcctcg ggcgactcct
     2581 cggggagcga tcgccaggcg cgcagcatct ggttgcgggc cgcgatcccc tgtgctcgca
     2641 cgtcggcgaa cgcgacgtcg tgcatatcgt cgtagaaggt cctgttcgtg gcgtcgatgt
     2701 cgagctcctc cggccgatag gtgccgaatc ggatccgctc gaggacggcc cctgcctcgg
     2761 cgagccagct tccgatgtga gcaaccagat ccttgaccga ccacccctcg acgaaatatc
     2821 cgggctcctc gaccttgtcc gccggaaggg cgtcgacgag tccgtgcagc tccgaccaca
     2881 gccgatcctc ctcggccaac aactcctgct tctgactcgg ctgcgacacg gcgcgcacag
     2941 tatgacgcgg gaacaaacgg cacggctcga gcgctgaacg gtgacatgga acgaacgacg
     3001 agggagacgc tcctgcggat cttcgaggag accgagacga tcgcggtggt gggtgcctcg
     3061 acgacggagg ggaagccggg tcacgacatc ccgctgtacc tccagtcgca gggctttgga
     3121 tcatcccggt gaaccctcga ggcggacgga tcctggggga accctcgttc gcgtcactgc
     3181 gcgacgtcgg cgtcccggtc gacgtcgtag acgtgttccg tccgcgggag gaggccggaa
     3241 cgatcgctcg tgacgcgatc gcgatcggcg cgaagacgct gtggttccaa cccgggaccc
     3301 acaccgacga agcggtggca caggccaggg cagccggcct cacggtcgtg gcccgcaggt
     3361 gcatgggggt gatccacggc tggctcggcc tgggcccggg gcctcacgtc ccctcggtcg
     3421 cctgacgtcg tccgtcggtc acgaagcggt gagcggtggc atcggcgttc cctcgccgtc
     3481 cagcaccgct tccagcgcgg tcaccagccc ctcgagctcg gtgcggctca ggtgggacag
     3541 gagatgctcc cgaagaccct ggacgtgaac gctcgtcgct gttcgtagca gcgcccggcc
     3601 ctcggcggtg agggtggcca ggtagccgcg cttgtcaccc tcgaagaccc tgcgctccac
     3661 gaggcccctg cgctcgagct gatcgacggc ccgggtcacg ccgccgggcg agagccggag
     3721 ccgccccgtc gaaccggcgg acggccgaca tcccttataa ggtcggagcg tggagtggtc
     3781 gccatcgttc tacgagctca gacgtgggat cggctgtgcg atgtgccagc agggccgccc
     3841 cgaagccacc gatgatggag tccgcttcta cgctggggag gtctcggacg cgtacctccg
     3901 tcgcaccaac atccagcgcg gcctcaccgt cgtgatctgg cggggcaggc atgttgccga
     3961 gcctaccgag ctcaacgatg aggaagcgca tggttattgg cgcgatctcc tcatcgtggg
     4021 ccgcaccctg gaagacgtgc tgcgacccgt caagacgaac tacaacgtgc tggggaacgc
     4081 tgttccgcat ctgcatacgc acatcgtccc tcgctacgct gatgaccctc gtcccgggtg
     4141 gccgttcccg ttcccggaag aagagccccc cgcgatgccc gaagaggagc ttcaacgaga
     4201 tctcgatgcc cttcgaatgg ccatcgatgc gaggtgatcc cgtcctgacc gaaccgggac
     4261 atcgcgacgt cctgatgtga gagaacgatc
//
LOCUS       DASUYD010000041         5836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_683312, whole genome shotgun sequence.
ACCESSION   DASUYD010000041 DASUYD010000000
VERSION     DASUYD010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683312"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VF195_02295"
     CDS             <1..1515
                     /locus_tag="VF195_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX6579687.1"
                     /translation="GRTQKARRRMLYEGGLEIYTTLEPDWQSWAEQEARRPLRVSISP
                     PPGSPPPDVSIVTIDNRTGAVKVILSGRNYEEEKLDLATTGHQPGSAFKPFVLAGAFK
                     RGIPPTQTYSSSSPWCSPAWDDEDHCVSNAEGSGVGLVDLYTATEDSINVVFAQLILD
                     VGAEVVADISEQMIGMDPTTEGLLAVPALATGSVAISPVDMAAGYQTIANDGRHCDPY
                     TVQSIERDGEVLLAHERACGPVLKQGDARLITSMLEAVPVSGTAASAFGAWGSWPVAG
                     KTGTAQENTNVWFGGYTKQFTTVVWVGSPGNPYSMGSVFGGTVAAPIWVSFMSRVMQG
                     LPAIGFPDPPKPPEGPVPSVIGMRRSEAISVLSEAGFRASVEVVDDSSPKGQVFSQSP
                     GGGSITALGTLIQIEISTGVPAKVTMPRVVDMRGYLAAEFLRSLGLVVNLVRVPTDDR
                     EKVGYVIAQDPRSTTEVVQGSTVKIVVGEEGNGGGNGNGGGNGNGNGGGNGGGG"
     gene            complement(1512..1973)
                     /locus_tag="VF195_02300"
     CDS             complement(1512..1973)
                     /locus_tag="VF195_02300"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEX6579688.1"
                     /translation="MSDPTLKERLAAEITGALRAGEKIRLGALRMLSAAVTNREKELR
                     HDLSDDELRDVAAKEMKKRAESIEAFEGAGREALAEKERAEREAIAPYAPEPMADADL
                     DALVDEAFAATGATSEKEFGAVMGFLMGKAKGRVDGKVVQEKVRARLGGTT"
     gene            1986..2873
                     /locus_tag="VF195_02305"
     CDS             1986..2873
                     /locus_tag="VF195_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6579689.1"
                     /translation="MFPVVVTVAALGVGCVLYGILVERFRYRVIRHRLDILPAAAHPL
                     VVLHLSDLHFVRHDPRKARFLAGLPTADVTVVTGDLLGEPEAIVTTVESVRAVRGRIA
                     SWFVLGSNDYFVPRPLNYLAYFRPGRPRRRAAPGRAPDLIRALVADGWDDLTDSRRSI
                     SLLGLETELLGLDDAHIGRHDLRIAPRSEPDRFGLAVMHSPDTAPEAAALGYGLIVAG
                     HTHGGQVRLPWIGALVTNCSMPRAFASGLVRMGSAVLHTSRGLGTSKYAPFRFWCRPE
                     ATYLDLHPGASGPGDDPLE"
     gene            2880..2953
                     /locus_tag="VF195_02310"
     tRNA            2880..2953
                     /locus_tag="VF195_02310"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2914..2916,aa:Pro,seq:cgg)
     gene            3102..4175
                     /locus_tag="VF195_02315"
     CDS             3102..4175
                     /locus_tag="VF195_02315"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX6579690.1"
                     /translation="MTGTRTRITRLVVAVVFALPVLLSALSIASLAAPSAEDVEAAKA
                     KLAGLQHEFEVLAEQYNDAKYRLTLIERKLAEARDQRDIAEQQAQRAESRLATRAVQA
                     YVGTGSQVDGLLGAESIAEFSDRLEFMGAVAEGDAEIARTASNARQEADWASDRFAAT
                     LTERQRQVATIENQLDRLDSMIGEQASLYEELNQDRQDYLAYVEAQRAALEQARLEAA
                     QDTLPADTSGDTGGYVPPVNGSAAQIAVQAALSVQGTQYVWGSADPNVGFDCSGLTSW
                     AWAQGGVYIPHSSAAQYSLPHVPLGSVQPGDLIFYYSPISHVALYIGGGQIVHATHPG
                     PDGAVHTDSMYGYDNPVGAARPG"
     gene            4280..4861
                     /locus_tag="VF195_02320"
     CDS             4280..4861
                     /locus_tag="VF195_02320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579691.1"
                     /translation="MGVHDLGRAPTTNDAPRRTHVRLDAGRRRGGGQETWLGVLRGLD
                     RFQGRSSLRTWIIRILTNIAKTRGQREGRSLPFASLAGDDLDAPAVDPDRFDTREDSS
                     RGAWSTLPDDWTGIPEDRLLGRETLAVIGRAIDGLPPMQAEVIRLRDALGWTSEEVRN
                     ALDLTETNQRVLLHRARAKVRSALEAYLSSETT"
     gene            4861..5103
                     /locus_tag="VF195_02325"
     CDS             4861..5103
                     /locus_tag="VF195_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6579692.1"
                     /translation="MGRDLTCKELVELVTGYLDGSLRGRLRRRFEAHLAACDGCTRYL
                     RQMEATIRMTGTLTEEQVTEEQQAVLLTAFRRWSAG"
     gene            complement(5100..5297)
                     /locus_tag="VF195_02330"
     CDS             complement(5100..5297)
                     /locus_tag="VF195_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579693.1"
                     /translation="MSLQPNEIFELIVKADEKLKYATADKTGTRTQQARDLLTQALDA
                     ARAIGNEQLVQQAELRLSDLA"
     gene            complement(5312..5446)
                     /locus_tag="VF195_02335"
     CDS             complement(5312..5446)
                     /locus_tag="VF195_02335"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579694.1"
                     /translation="MPGVFAAGDVRDGSTKQLASAVGEGAAVAIQIRGYLDRLDRRSM
                     "
     gene            complement(5421..>5836)
                     /locus_tag="VF195_02340"
     CDS             complement(5421..>5836)
                     /locus_tag="VF195_02340"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6579695.1"
                     /translation="GSDELMVIGGGNSALEEGIFLTQFTERVTIVERGDRLRGSRILQ
                     AKVLDHPRMKVELRHGVKEFRTKGDGSGKLGSVVIEDLVSGALEEQHPAGVFVFIGLD
                     PNTGFVKGRPRSGRARVRRHRSDVHELDARCVRRG"
BASE COUNT          886 a         2045 c         1976 g          929 t
ORIGIN      
        1 gggaggaccc agaaggcgcg ccgccggatg ctgtacgagg gcggcctcga gatctacacc
       61 accctcgaac ccgactggca gtcgtgggcc gagcaagagg cacggaggcc gctgcgggtc
      121 tcgatcagtc cgccgcccgg ctcaccgcct cccgatgtct cgatcgtcac gatcgacaac
      181 cggacgggcg cggtgaaggt gatcctctcc ggccggaact acgaggagga gaagctcgac
      241 ctcgcgacca ccggccatca gcccggatcg gccttcaagc cgttcgtgct cgccggagcc
      301 ttcaagcggg gcatcccgcc cacgcagacc tactccagct cctcgccgtg gtgctcgcct
      361 gcctgggacg acgaggatca ctgcgtctcg aacgccgaag gcagcggggt cgggctcgtc
      421 gacctgtaca cggccaccga ggactcgatc aacgtcgtgt tcgcgcagct catcctcgac
      481 gtcggcgcgg aggtggtcgc ggatatctcc gagcagatga tcgggatgga tcccacgacg
      541 gagggcttgc tcgcggtgcc ggccctcgcg acggggtcgg tggccatctc gcccgtcgac
      601 atggccgcgg ggtaccagac gatcgcgaac gacggccggc actgtgaccc ctacaccgtc
      661 caatcgatcg agcgggacgg cgaggtgctc ctggctcacg agcgcgcctg cggtcccgtg
      721 ctcaagcagg gcgacgcgcg actcatcacg tcgatgctcg aggccgtgcc ggtctcgggc
      781 acagcggcca gcgcgttcgg cgcatggggc agctggcccg tcgccggcaa aaccggcacg
      841 gcgcaggaga acacgaacgt ctggttcggt gggtacacga agcagttcac gaccgtggtc
      901 tgggtcggct cgccggggaa tccctactcg atgggctcgg tcttcggcgg caccgtcgcg
      961 gcgccgatct gggtgtcgtt catgtcgagg gtgatgcagg gactgccggc gatcgggttc
     1021 cccgaccccc cgaagccgcc cgaggggccc gtcccgagcg tcatcggcat gcgccgcagt
     1081 gaagcgatct cggtcctgtc ggaggccggt ttccgcgcgt cggtcgaggt ggtggacgac
     1141 agcagcccga agggccaggt cttcagtcag tcccccggtg gcggatcgat caccgcgctc
     1201 ggcacgctca tccagatcga gatctcgacc ggtgtcccgg ccaaggtcac gatgcctcgg
     1261 gtggtcgaca tgcgcggcta cctggcggcg gagttcctcc gatcactcgg gctcgtcgtc
     1321 aacctcgtgc gggtgccgac ggacgatcga gagaaggtcg ggtacgtgat cgcccaggat
     1381 ccccgcagca cgaccgaggt cgtccagggc tccaccgtga agatcgtcgt gggcgaagag
     1441 gggaacggcg gtggcaacgg gaacggcggt ggcaacggga acggcaacgg tggagggaac
     1501 ggcgggggag gctaggtcgt cccgcccagc ctggcgcgca ccttctcctg gacgaccttc
     1561 ccgtccacgc ggcccttcgc cttgcccatg aggaaaccca tgacggcgcc gaactccttc
     1621 tccgacgtgg ctccggtggc cgcgaaggcc tcgtccacca acgcatccag gtcggcgtcg
     1681 gccatcggct ccggcgcgta gggagcgatc gcttcccgct cggctcgctc cttctcggcg
     1741 agcgcctccc gcccggcgcc ctcgaaggcc tcgatcgatt cggctcgctt cttcatctcc
     1801 ttcgcggcga cgtcgcggag ctcgtcgtcg gagaggtcgt gccggagctc tttctcccgg
     1861 ttggtgacgg ccgccgagag catgcggagc gcgccgagac ggatcttctc gcccgcgcgg
     1921 agggcaccgg tgatctccgc ggcgaggcgc tccttcagcg tcgggtcgct cactacactc
     1981 cccccgtgtt ccccgtggtc gtgacggtcg ccgcgctcgg cgtcggctgc gtgctctacg
     2041 ggatcctcgt ggaacggttc cggtaccggg tgatccggca ccgcctggac atcctaccgg
     2101 cggccgcgca ccccctcgtc gtgctgcatc tgtccgacct ccacttcgtt cgtcacgacc
     2161 cccggaaggc gcgcttcctg gcagggttgc ccacggcgga cgtcacggtc gtgaccgggg
     2221 atctcctggg tgagccggaa gcgatcgtca cgacggtcga gagcgtgcgg gcggtgcgcg
     2281 gtcgcatcgc gtcgtggttc gtgctcgggt cgaacgacta cttcgtgccc cgaccgctca
     2341 actacctggc gtacttccgt ccggggcgtc ctcgccggcg agcggcacct ggtcgtgcgc
     2401 ccgatctgat ccgggccctc gtcgcggacg gatgggatga cctcacggac tcgcgacgct
     2461 cgatctcgct cctcgggctc gagaccgagc tgctcggtct ggacgacgcc cacatcgggc
     2521 gccacgacct ccggatcgct cctcggagcg agcccgatcg cttcggcctc gccgtgatgc
     2581 actctcccga cacggctccg gaggcggccg cgctggggta cgggttgatc gtcgccggac
     2641 acacccacgg cggccaggtt cggctcccgt ggatcggcgc gctggtgacc aactgctcga
     2701 tgccgcgggc gttcgcgtcg gggctggtcc ggatggggtc cgcggtgctc cacacgtccc
     2761 gaggcctcgg aacgagcaag tacgcgccgt tccggttctg gtgccggccg gaggccacgt
     2821 acctcgacct tcatcccggc gcgtcgggac cgggtgacga cccgctagaa tgagggactc
     2881 gggacgtggc gcagcttggt agcgcgcagc gttcgggacg ctgaggtcgc gggttcaagt
     2941 cccgccgtcc cgacaccgat acacggggcg ggccggaaac ggtccgcccc acgcatgttc
     3001 gctatcgcgc aacaggggtt tgacgacccc tggaggcacc ggtagcctcg ggcgacggct
     3061 ccggcgacgg agcctttcgt actactcgga cctaggagca gatgaccggg acgcgaacgc
     3121 ggatcacacg gctcgtcgta gccgtggtct tcgcgctccc cgttctcctc tccgcgctca
     3181 gcatcgcctc cctcgcggcg ccctccgccg aagatgtcga ggcagcgaaa gcgaagctcg
     3241 cgggactgca gcacgagttc gaggtcctcg ccgagcagta caacgacgcg aagtaccgtc
     3301 tcacgctgat cgagcgcaag ctcgcggagg cgcgcgatca gcgtgacatc gcggagcagc
     3361 aggcgcagcg cgcggagtcc cgcctggcga cgcgagcggt gcaggcctac gtgggaaccg
     3421 gttcccaggt cgacggactc ctcggcgccg agagcatcgc agagttctcg gaccggttgg
     3481 agttcatggg tgccgtcgcc gaaggtgacg cggagatcgc ccgtacggcg tcgaatgctc
     3541 ggcaggaagc cgattgggcg tcggatcggt tcgccgcgac cctcacggag cgacagcgac
     3601 aggtcgccac gatcgagaac cagctcgatc ggctcgattc gatgatcggg gagcaggcgt
     3661 ccctctacga ggagctgaac caggatcggc aggactacct cgcctacgtt gaggcgcagc
     3721 gggccgctct cgagcaggca cggctggagg cggcacagga cacgctaccg gcggacacct
     3781 cgggcgatac cggtgggtac gttccgccgg tcaacggttc ggccgcccag atcgcagtac
     3841 aggcggctct gtcggttcag gggacgcagt acgtgtgggg ctccgccgat ccgaacgtcg
     3901 gcttcgactg ctccggcctc acctcgtggg cgtgggcgca ggggggcgtg tacatcccgc
     3961 actcgtcggc cgcccagtat tccctcccac acgtgccgct cggcagtgtg cagccgggtg
     4021 atctgatctt ctactactcg ccgatcagcc acgtggcgct gtacatcggt ggcggacaga
     4081 tcgtccatgc cacgcatccg ggccccgacg gagccgtcca cacggactcg atgtacgggt
     4141 acgacaaccc cgtcggcgcc gcgcgaccgg gctagcctcc cgctccatcg cacccgcgag
     4201 taacctcctc gctcgtgacc gaaccgcccg ctccccgcga cgacctcgct ctggtggaaa
     4261 ggctccgcgc cggggacgga tcggcgttca tgaccttggt cgagcgccta caaccaacga
     4321 tgctccgcgt cgcacgcatg tacgtctcga cgccggccgt cgccgaggag gtggtcagga
     4381 gacgtggctc ggcgtccttc gcggcctcga ccgcttccag gggcggtcgt ccctgcgcac
     4441 gtggatcatc cggatcctga cgaacatcgc gaagacgcgc ggccagcgcg aagggcggag
     4501 ccttccgttc gcctcgctcg ccggcgacga cctggacgca ccggccgtgg atcctgatcg
     4561 cttcgacacg cgcgaggact catcgcgggg ggcctggtcg acgctgccgg acgactggac
     4621 cggcatcccg gaggatcgtc ttctcggtcg cgagaccctc gccgtcatcg gcagggcgat
     4681 cgacgggctc cctccgatgc aggccgaagt gatccggttg cgagacgcct tgggctggac
     4741 gtcggaggag gtccgtaacg cgctcgacct gaccgagacc aatcagcggg tgctcctgca
     4801 tcgcgcacgc gcgaaggtgc ggagcgcact ggaggcatac ctctcgagcg agacgacgtg
     4861 atgggacgcg acctgacctg caaggagctg gtagagctgg tgaccggcta cctcgacggc
     4921 agcctgcgag gccggctccg gcggcggttc gaggcgcacc tcgccgcgtg cgacgggtgc
     4981 acccggtacc tccgccagat ggaagccacg atccggatga ccggcaccct caccgaggag
     5041 caggtgaccg aggaacagca ggccgtcctc ctgacggcgt tccgccgctg gtcggcgggc
     5101 taagccagat ccgagagtcg cagctccgct tgctgcacca gctgctcgtt cccgatcgca
     5161 cgcgcggcgt cgagagcctg tgtgagcaga tccctcgcct gctgcgtcct cgttcccgtc
     5221 ttgtccgcgg tggcgtactt cagcttctcg tccgccttga cgatcagctc gaagatctcg
     5281 ttcggctgca gggacatctc gacatcatcc ctcacatcga gcggcggtcg agccggtcaa
     5341 ggtagccgcg gatctggatc gccaccgccg cgccctcgcc caccgccgag gcgagctgct
     5401 tcgtcgagcc gtctcgcacg tcacccgcgg cgaacacacc gggcatcgag ctcatgaacg
     5461 tcggatcggt ggcgacgaac ccgcgctcgt ccagatcgag gcctgccctt cacgaagccg
     5521 gtgttcgggt cgagaccgat gaacacgaac acgcccgcgg ggtgctgctc ctccagcgcg
     5581 cccgagacga gatcctcgat cacgaccgac ccgagcttcc cggaaccgtc ccccttcgtc
     5641 cggaactcct tcaccccgtg ccgcagctcg accttcatcc gtgggtgatc cagcaccttc
     5701 gcctgcagga tcctggaccc ccgcaatcgg tcgcctcgct ccacgatcgt gacccgttcg
     5761 gtgaactggg tcaggaagat cccctcctcg agggcggagt tgccgccgcc gatcaccatc
     5821 agctcgtcgg agccct
//
LOCUS       DASUYD010000042         6541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_242539, whole genome shotgun sequence.
ACCESSION   DASUYD010000042 DASUYD010000000
VERSION     DASUYD010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6541
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242539"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VF195_02345"
     CDS             <1..847
                     /locus_tag="VF195_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6579696.1"
                     /translation="DRNKVVMSELDFPTDGHVWLANAKRGVEIEWLRSPDGLTIPLEE
                     YDRAIDERTAVVMVNRVLYRSSAIVDAKEVCRIARDRGALSFLDDYHGIGIVPLDLHD
                     VGCDVYAAGVLKWLCGGPGLAFLYVRRALIPSLEPAVTGWFGTAEPFSFDTQHLEYHP
                     TARRFEHGTPPAPVSFIAQGGLDVITEVTPERIRARQGELGDHLIARADELGLPVRTP
                     RERNARGGVVNVGVGPEAGKICHALLERDVCTDYRGDGLRISPHFFNTEEDIDRCFEE
                     LRTLL"
     gene            complement(854..1951)
                     /locus_tag="VF195_02350"
     CDS             complement(854..1951)
                     /locus_tag="VF195_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX6579697.1"
                     /translation="MRDSTTDLPEGVSASSELTMDELQLAQRNHGMHLEGLRYEVTPV
                     GMHYLLIHFDVPDAEESAWTLSIGGLVHRPLDLSMAELRVRPSVTFPVTMECAGNGRA
                     RLSPRPISQPWLTEAIGTAEWSGTPLRPLLEEAGVRDEAIELVFAGADRGIQGEVEQD
                     YERSLSIADAMREEVILAYEMNGEPLPPQHGYPLRLLVPGWYGMTSVKWLTRITAVAE
                     PFEGFQQLAYRYRQQPGDDGMPVTRMEPRSLMIPPGIPDFLTRSRILDAGLHELRGRA
                     WSGWGPIERVQVSADGGSTWETAELGDAVSPHAWRFWSFDWDAQPGTFELCCRAADGA
                     GNEQPLEPSWNLGGFSNNSVQRVSVQVRPRV"
     gene            complement(1968..4646)
                     /locus_tag="VF195_02355"
     CDS             complement(1968..4646)
                     /locus_tag="VF195_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6579698.1"
                     /translation="MDLQPGTEVAGYRIVSTLGRGGMSVVYLAEHDWLQRKVALKVLA
                     PQLAEDERFRERFVRESRLAASLDHPNVIPIYEAGASGGDLFIAMRYVEGTDLRTLLH
                     EGRALEPGRAVAIVRQVAAALDAAHEQGLVHRDVKPGNVLLARQRGSEAGEHVYLSDF
                     GLTKRSASDSGITGTGQFVGTLDYAAPEQFKGGTPDARTDVYSLGCVLFECLTGRPPF
                     QSDNDAGLMYAHLQQAPPAVTGIDPDLPREIDAVVATAMAKEPAYRQESAGALSHEAS
                     RALGAEASTTGAEGRTRLRRSAPALAVAAALVVGVVVSSFFQGEPPAQSRGRSASVPT
                     ASPTLPPTFQTVERGLSDAEEQLLVYVPDDVSSECLPFDRTEPIHGERATLVCRTAEI
                     EVLYELFQTRDEMDSAFEVNMNNKQAPEGECATEHLAVTEYTIDSKPAGRVLCYTVKR
                     GPFTRQLPDHIEWTDENASIYAHAVRNDLGDLSLYEWWLSSAGPLVSSNEGAPEKDRP
                     AAVAGPRLRDGTYLLSLTHAEAAAAGGSSNAIRLNEGTYQYVIDGDVPEEGRTYLQKP
                     NAIVFAPLRCPEESSGASAALEPVVYGWSVKGDSVSWNKLRGGSCAGPQPNFVPSRPW
                     TRAPAGLIAVEEGGEIELVSPGGAIVGSTTQTDTNPNRWPDWSPDGSRIVFAGAGQDG
                     YDLYVMNADGTDLQQITGDPGDEIAPAWSPDGKKIVFSFDDGGDPDFRSGLATVRPDG
                     SARTELFARTNEVTEIPAWSPDGGSLAFTVFTAEGFVPYVMDTDGGRLTRLREDPGLT
                     LDWTRDGRRVLLYGDGSFLSVRPDGSGERVFFEEPPEGGRLVIDWSPDGDWLVMSSVT
                     GSSLEPRNNLYLMSADGSQLFQIGLGSEPSWRPETG"
     gene            complement(4728..4787)
                     /locus_tag="VF195_02360"
                     /pseudo
     CDS             complement(4728..4787)
                     /locus_tag="VF195_02360"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5121..5429)
                     /locus_tag="VF195_02365"
     CDS             complement(5121..5429)
                     /locus_tag="VF195_02365"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6579699.1"
                     /translation="MEFLILGPLEVADEGRKLALGGPKQRAVLAHLILRANRVVRADL
                     LIDGLWGEEPPESARNTLQTYVYRLRKLLGENRIEGRDGGYVLSPLPTRSTVCDSSRS
                     "
     gene            5560..6153
                     /locus_tag="VF195_02370"
     CDS             5560..6153
                     /locus_tag="VF195_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579700.1"
                     /translation="MDTATRTHSSRIALFLCVVVSAAILGSQRPAPARPQPLTVHGAT
                     AAEQRTIDWAIRRYHTAGLDGMPNLEVVLHRSDEGCNGGIGLYYSGRIDLCTKDASEP
                     YLRKFALHEMAHAWTEANIDAQILDRFMDVRGIAAWNDRSFDWKERGTEQVAEIITWG
                     LGQGEIAPLLPEITEPETLARLYRLLTARDPITPAAR"
     gene            complement(6157..>6541)
                     /locus_tag="VF195_02375"
     CDS             complement(6157..>6541)
                     /locus_tag="VF195_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6579701.1"
                     /translation="SDPLLEAAARTMRMVSTLDILSFGDVTQELRTACDNLVRFRTAG
                     GTVVYGTDLGNGAIPPGIHLREALLLHEVVRMKPEEVLVAMTAGPIAVDGPADLIVLG
                     SDPLVRLEALGDLRTVVRAGRVVSG"
BASE COUNT         1024 a         2327 c         2175 g         1015 t
ORIGIN      
        1 ggaccggaac aaggtcgtga tgtccgagct ggatttcccc accgacgggc acgtctggct
       61 cgcgaacgcc aagcgcggcg tcgagatcga gtggctccgt tcacccgacg ggctcacgat
      121 ccccctcgag gagtacgacc gcgcgatcga cgaacgcacg gccgtcgtca tggtgaaccg
      181 cgtgctctac cgctcgagcg cgatcgtcga cgcgaaggag gtctgccgga tcgcgcgcga
      241 tcgcggcgcg ctctcgttcc tcgacgacta ccacgggatc ggcatcgttc cgctcgacct
      301 tcacgacgtt ggttgcgacg tctacgcggc aggcgtcctg aagtggctct gcggcggtcc
      361 gggtctcgcg ttcctatacg tccggcgggc gctgatcccc tcgctcgagc cggcggtgac
      421 gggatggttc gggacggccg agccgttctc gttcgacacg cagcacctcg aataccaccc
      481 gaccgctcgc cgcttcgagc acgggacgcc accggcgccg gtctccttca tcgcccaggg
      541 cgggctcgac gtcatcaccg aggtcacgcc cgaacggatc cgcgcccgtc agggcgagct
      601 gggcgaccac ctcatcgctc gagccgatga gctcgggttg ccggtgcgga ccccgcgcga
      661 gcggaacgcg aggggcggcg tcgtcaacgt cggtgtcggg cccgaggcgg ggaagatctg
      721 ccacgccttg ctcgaacgcg acgtgtgcac ggactaccgg ggggacggcc ttcggattag
      781 cccgcacttc ttcaacaccg aggaggacat cgaccgctgc ttcgaggagc tgcggacgct
      841 gctctgatcg acgctacacc cgagggcgga cctggacgga gacgcgctgt accgaattgt
      901 tcgagaaccc gccgaggttc cacgacggct cgagcggttg ttcgttgccg gctccgtcgg
      961 ccgcccggca gcaaagctcg aaggtgccgg gctgcgcatc ccagtcgaac gaccagaaac
     1021 gccacgcgtg gggcgagacc gcatcgccga gctccgcggt ctcccatgtc gagcccccgt
     1081 ccgcactcac ctgcacccgc tcgatcgggc cccacccgga ccacgcacga ccgcgcagct
     1141 cgtggagccc ggcgtccagg atgcggctcc gcgtcaggaa gtccgggatg ccgggcggga
     1201 tcatgagcga ccggggttcc atccgcgtca cgggcatccc gtcgtcccct ggctgttggc
     1261 ggtaccgata cgcgagctgc tggaaaccct cgaacggctc ggccaccgcc gtgattcgcg
     1321 tcagccactt cacgctcgtc atgccgtacc aaccggggac gaggaggcgg agagggtagc
     1381 cgtgctgcgg cgggagcggc tcgccgttca tctcgtaggc gaggatcacc tcctcgcgca
     1441 tcgcgtcggc gatcgacagg cttcgctcgt aatcctgctc cacctcgcct tggatgcctc
     1501 ggtcggctcc ggcgaacacg agctcgatgg cctcgtcccg aacgccggcc tcctcgagca
     1561 acggacggag cggcgtcccg ctccattcgg ccgtgccgat ggcctcggtc agccacggtt
     1621 gcgagatggg acgcggtgac agcctcgccc gtccgttgcc cgcgcactcc atcgtcaccg
     1681 ggaacgtgac gctcggtcgg acacggagct cggccatcga caaatcgagc ggccggtgaa
     1741 cgagacctcc gatcgagagc gtccacgccg attcctccgc gtcaggaacg tcgaagtgga
     1801 tcaagaggta gtgcatcccc accggcgtga cctcgtagcg gagcccctcg aggtgcatcc
     1861 cgtggttgcg ctgggcgagc tgcagctcgt ccatcgtcag ctccgaggag gccgacacac
     1921 cctccgggag gtccgtcgtt gaatcccgca tatcgctcac cccgccgtca tcctgtctcg
     1981 ggacgccacg acggctccga ccccaacccg atctggaaca gctgacttcc atccgcgctc
     2041 atgagataca gattgttcct gggctccaag ctcgagccag tcaccgacga catgacgagc
     2101 caatcaccat cgggcgacca gtcgataacg agccgaccgc cctcaggtgg ttcctcgaag
     2161 aacacgcgtt ctcccgatcc gtccggccgc accgatagga acgacccgtc gccgtagagg
     2221 aggacccgcc gcccatcccg tgtccagtcc agggtgagcc cagggtcctc tcgcagtctc
     2281 gtgagtcgac cgccatcggt gtccatcacg taagggacga acccttccgc cgtgaacacg
     2341 gtgaacgcga ggctgccccc gtccggagac cacgccggga tctcggtgac ctcgttcgtt
     2401 cgcgcgaaca gctccgtcct cgccgaacca tccggcctta cagttgccaa tcctgatcga
     2461 aagtccgggt cgcccccgtc gtcgaacgag aacacgatct tcttcccgtc gggggaccat
     2521 gccggcgcga tctcgtcgcc gggatcgcct gtgatctgtt ggaggtcggt gccgtccgcg
     2581 ttcatcacat agaggtcgta cccatcctgc cctgcgcccg cgaagacgat cctcgatccg
     2641 tccggcgacc agtccggcca cctgttcgga ttggtatcgg tctgggtggt ggacccaacg
     2701 atcgctcccc ccgggctcac gagctcgatc tccccaccct cctcgacagc gatcagcccg
     2761 gccggggccc gggtccacgg gcgggagggc acgaagttgg gttgcggtcc ggcgcacgag
     2821 ccgcctcgca gcttgttcca ggagaccgag tcccccttca cggaccaccc atacaccaca
     2881 ggctccagag cggccgaggc acccgatgac tcttccgggc acctcagggg cgcgaacacg
     2941 atcgcgttcg gtttctggag atacgtcctc ccctcttctg ggacgtcccc gtcaatgacg
     3001 tactgatacg tgccctcgtt gagccggatg gcattggacg accctccggc cgcggccgcc
     3061 tccgcatggg tcagggaaag gaggtaggtc ccgtcgcgga ggcgtggacc ggccaccgcc
     3121 gccgggcggt ccttctccgg cgcaccctcg ttcgaagaaa cgagcggtcc ggccgacgac
     3181 agccaccact cgtacaggct gaggtcgccg agatcgttcc ggaccgcgtg cgcgtagatc
     3241 gaggcgttct cgtccgtcca ttcgatgtga tccgggagtt gtcgggtgaa cggccctcgc
     3301 ttcaccgtgt aacacaggac tcgaccggcc ggcttactgt cgatcgtgta ctcggtgacg
     3361 gcgaggtgtt cggtggcaca ctcgccttca ggcgcttgct tgttgttcat gttgacctcg
     3421 aacgcagaat ccatctcatc acgggtctgg aacagctcgt acagcacctc gatctcagcg
     3481 gttcggcaca cgagggtcgc gcgctcgcca tggatcggtt cggtgcgatc gaatgggagg
     3541 cactcggagc tcacgtcatc gggaacgtac acgagcagct gttcctcggc gtccgagaga
     3601 ccgcgctcga ccgtctggaa ggtgggcggc aaggtcgggc ttgccgtggg caccgaggcg
     3661 gatcggcccc ggctctgcgc cggcggctcc ccttggaaga acgaggagac caccaccccc
     3721 accacgagcg ccgccgctac ggccaacgcg ggcgcggaac ggcggagccg cgtgcgtcct
     3781 tcggcgccgg tcgtcgaggc ctctgcgccg agtgcgcgcg acgcctcatg gctcaaggct
     3841 cccgcgctct cctgccgata cgcgggttcc ttggccatcg cggtggcgac cacagcgtcg
     3901 atctcccgcg ggaggtcggg atcgatcccc gtgaccgcgg gcggcgcctg ctggagatgc
     3961 gcgtacatca gcccggcgtc gttgtccgac tggaatggcg gacgtcccgt caggcattcg
     4021 aacagcacgc acccgagcga gtacacgtcg gtgcgcgcgt cgggcgtgcc gcccttgaac
     4081 tgttcgggcg ccgcgtagtc gagcgtcccg acgaactgcc cggtcccggt gatccccgaa
     4141 tcgctcgccg agcgtttcgt gaggccgaag tcggagaggt agacgtgctc ccccgcctcc
     4201 gagccccgtt gccgcgcgag gagcacgttg cccggtttga cgtcccggtg gacgaggcct
     4261 tgctcgtgtg cggcgtcgag ggccgccgcg acctgccgga cgatcgcgac ggccctgccc
     4321 ggctcgagcg ctcgaccttc gtggagcaac gtacggagat cggtcccctc cacgtaccgc
     4381 atcgcgatga acaggtcgcc gcccgaggcg cccgcctcgt agatcgggat cacgttcggg
     4441 tggtcgagcg aggccgcgag ccgcgactcg cggacgaatc gctcccggaa ccgctcgtcc
     4501 tcggccagct gcggcgcgag gaccttgagc gcgaccttcc gttggagcca gtcgtgctcg
     4561 gcgaggtaga cgacgctcat ccccccgcga ccgagggtgc tcacgatccg gtagccggcg
     4621 acctcggtgc ccggctgcag atccacccgg ctcgagcgaa ccgtgggcgg gcgagcgcgg
     4681 tctggtcggg gcgctgcgag gttcggggct cgcgtgagga tctgttcgtg caggcgctgg
     4741 agctcgggcg agggatcggc cccgagctcg tcgccgagca cctgtcgagc gcgctcgtag
     4801 ggtcgatagc gcttgggcct ggcggccgga tcggtataac gcgagcatca ggctcgccca
     4861 catccgctcc cggagcgggt agcgggccgt gagcgactcg agctccgaga ccacggtgga
     4921 gtgcccaccc attgcgatct ccgcggcgat gcggtgctcg gtggccgcga gccgaagctc
     4981 cgccagacgc gcggcctcgc cccggagcga cggctcctcg gtcacgtcgg cgagcgcgtc
     5041 ggcccgccac agggagagcg cgtcggcgag gatcgccgcc gccgcggcgg gatcggaggc
     5101 gatcgaggcc ttgccctcct tcacgagcga ctcgaatcgc acaccgtcga tctcgtcggg
     5161 agcggcgaca gcacgtaccc tccgtcacgg ccctcgatcc ggttctcccc caggagcttg
     5221 cgcaggcggt agacgtacgt ctgcagggtg ttgcgggcgc tctcgggagg ctcctccccc
     5281 cagaggccat cgatcagcag gtcggcccgg acgacccggt tcgcccggag gatcaggtgg
     5341 gcgagcaccg cgcgctgctt cggacctccg agtgcgagct tgcgcccttc gtcggcgacc
     5401 tcgagcggac cgaggatcag gaactccatc gccgcgaacc gtacgacggc gtgtcctcga
     5461 acggaagcca cgcaggcgtc cgcgtgacgt ccgtcacgtt ccagcgaact gcgaccggat
     5521 tgcggcaggt ctgccagggc accggacgag ggtgcggcca tggacaccgc gacccgcacc
     5581 cacagcagca ggatcgccct cttcctctgc gtggtcgtct cggccgcgat cctcgggagc
     5641 cagcggcccg ccccggctcg gccacagccc ctgacggtcc acggagccac cgccgccgag
     5701 cagcggacga tcgactgggc gatccggcgc taccacaccg ccgggctgga cgggatgccg
     5761 aacctcgagg tcgtcctgca ccgctcggac gaggggtgca acggcggcat cggcctctac
     5821 tactcgggcc ggatcgacct gtgcaccaag gacgcgagcg agccgtacct gaggaagttc
     5881 gcgcttcatg agatggcgca cgcgtggacc gaggccaaca tcgacgccca gatcctcgat
     5941 cggttcatgg acgtccgtgg gatcgccgcc tggaacgacc ggagcttcga ctggaaggag
     6001 cgggggaccg agcaggtggc cgagatcatc acctgggggc tcggtcaggg cgagatcgcg
     6061 cccctgctcc ccgagatcac cgagcccgag acgctcgcca ggctctaccg actcctcacc
     6121 gctcgcgatc ccatcacacc ggctgcgcgg tgagtgctag cccgagacca cccgacccgc
     6181 ccgaacgacg gtgcggaggt cgccgagcgc ctcgagccgc accagcgggt ccgaccccag
     6241 cacgatcagg tcggccggcc cgtcgacggc gatcggaccc gcggtcatcg cgaccaggac
     6301 ctcctcgggt ttcatccgaa ccacctcgtg caggagcagg gcctcgcgga ggtggatccc
     6361 gggcgggatc gcgccgttgc ccaagtccgt cccgtagacc acggtccctc ccgcggtgcg
     6421 gaaccggacg aggttgtcgc acgcggtccg gagctcctgc gtcacgtccc cgaacgacaa
     6481 gatgtcgagg gtcgacacca tccgcatcgt tcgcgcggcc gcctcgagca gcggatcgga
     6541 c
//
LOCUS       DASUYD010000043         6718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_463909, whole genome shotgun sequence.
ACCESSION   DASUYD010000043 DASUYD010000000
VERSION     DASUYD010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463909"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..137)
                     /locus_tag="VF195_02380"
     CDS             complement(<1..137)
                     /locus_tag="VF195_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX6579702.1"
                     /translation="MTLTKEARQQIIQEHALSSGDTGSADVQVAVLTTRIAELTEHLK
                     T"
     gene            198..839
                     /locus_tag="VF195_02385"
     CDS             198..839
                     /locus_tag="VF195_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX6579703.1"
                     /translation="MACVWRSDSLRTACGILGPAAFTGAWLLAQQRQEEYQVRHEHIS
                     GLAARDATDPYLMTAGFLALGSCTVAFASSLEERLGPRPGLGPTLIGVAGLATISAGL
                     FRRDRRSNYPPPGVPDGQSWVNDMHDLAAAVGGAAGTAGLVALAPALARDPALRDLAR
                     PAIGAAGASAGISAWFVRDVVRPGNGLLQRANVTIPLVFMARLAIRLMKDARE"
     gene            complement(857..2236)
                     /locus_tag="VF195_02390"
     CDS             complement(857..2236)
                     /locus_tag="VF195_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579704.1"
                     /translation="MIPRYSGVRARVAIFALLIAAAGLGFVVDAFRGRPEVREPVVPL
                     GTGTIAYVAFDGATLKWSLFTVEADGSSDMRIAVDLPGEAFHPSWSPDGTKLAFDVQS
                     GGDTEIYVATIYGSNGSNLSKLTTTPGWNFLPAWSPGSRIAYVHTSGPNHDIWLMNAD
                     GSNPVRLTHDPDFDLNPTWSPDGTKLAFESNRTASPEIYVMNADGSHVSRLTRAPGFD
                     GSPAWSPDGRQIAFVSDRDEPGIYLMASDGSHVRKLLAGDGDGPLDPEWSPDGALLAY
                     SSSPGPEIGPAISVIDPVSGHTRVITETAGELCCPSWRRGHEPVTGTAMPGTCDYGPW
                     MELCPEASWARHVLGEAGYPNVDDTGTVFEVPVAGGPVTFNFWGTDPLNHGQVQPIGA
                     FISAEDVPVTTRIHGVAVYRIDESWAWSTRGLNVWARAANPEDDPPGLSLAIVRVLVL
                     ASQRVPYPP"
     gene            complement(2334..2498)
                     /locus_tag="VF195_02395"
                     /pseudo
     CDS             complement(2334..2498)
                     /locus_tag="VF195_02395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
     gene            complement(2960..3895)
                     /locus_tag="VF195_02400"
     CDS             complement(2960..3895)
                     /locus_tag="VF195_02400"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEX6579705.1"
                     /translation="MDTVRGLQALPLPDGPSVVTVGFFDGVHLGHQEVFRRTVEAAKE
                     RDARSVAATFDRHPRETLTPGEEPRLLTTVDRKASLIEACGVDVLVVLEFTTAFSQVS
                     AEDFVRDVLAGGLHAIHARVGANFTFGHRAAGTVKTLREVGEPLSMTADEVDLFELDG
                     RVVSSSSIREALASGDLAWPRRALGRRYAVDGEVVAGAGRGKGLGYPTANLRTWPRLL
                     LPGQGIYAGVAELQDGGRHLAAIDVGTNPTFGTEPLHVEAFLLDFPDDELRGDTLTVE
                     FWERLRDEVKFDSVHELIAAIAEDVERTRSVVGSI"
     gene            complement(3936..4781)
                     /gene="truB"
                     /locus_tag="VF195_02405"
     CDS             complement(3936..4781)
                     /gene="truB"
                     /locus_tag="VF195_02405"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020458816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX6579706.1"
                     /translation="MTAAEGLLLVDKPAGITSHDVVDSVRRTLATKKVGHAGTLDPMA
                     TGLLLIGVGRATRLLRFLGDLAKTYEGTLRLGVETDTLDADGDVIRESSVDVTKEQVG
                     VAMRALVGESLQRPPAYSAVKVGGRKLYEAARAGERLEAEPRPISVETFELISFEPPD
                     ATFRVVCSGGTYIRVLAADVGAAVSTGAHLTTLRRTAIGPYRVEDAATPDSAGDPFPI
                     EAAVGHLPRLDLDAHEAAAASHGRPLGPAGLTGPYGVFGPNGRLIGVYEDDGPRARPH
                     VILAG"
     gene            complement(4778..5773)
                     /locus_tag="VF195_02410"
     CDS             complement(4778..5773)
                     /locus_tag="VF195_02410"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEX6579707.1"
                     /translation="MPDADARIELRRAGEVLAAAEEVALACHLNPDADALGSMLGLSN
                     FLRARGVATVCSFANEPFDLPRWIALLPGADRLVPPAAFPAESPVMVTCDCASFDRLG
                     VLGKAASRAGELIWIDHHRSNDGVGTVRLVDPDASSTCEMVFRLIETMGGDFDRDVAV
                     CLYAGLVTDTGRFQYQATTPETLRIAARLREQDFDHARLVRAMYEDNEPGFLRLLGTA
                     LGRMTEVPEASMVWTYLTQADLADAGVVAGETDDVIDVLRTARDVDIAAVLKQQRDGR
                     FKVSVRSRGGHDLSAMASAFGGGGHRLAAGYTSEHGPEGTIERLVAALRQAPVPS"
     gene            complement(5766..6152)
                     /gene="rbfA"
                     /locus_tag="VF195_02415"
     CDS             complement(5766..6152)
                     /gene="rbfA"
                     /locus_tag="VF195_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEX6579708.1"
                     /translation="MTQGARTARVAEEFREVLAEEIQKLKDPRIGFVTVTGVQVTADL
                     RRAWVSYTVLGDDKQQRSTAAGLRSATPHLRQALGKQVRLRNLPELEFQVDQTLAQHQ
                     HIEEIIERLRHPEDQQPEDQKEQPDA"
     gene            complement(6175..6462)
                     /locus_tag="VF195_02420"
     CDS             complement(6175..6462)
                     /locus_tag="VF195_02420"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEX6579709.1"
                     /translation="MAALRIDLRIPACGSLKEKRHVIKTLSNGLRSTFPVAVAETGYQ
                     DMWQRAELGVAAVGSEGHHLKKVMHGVERFVERWAEVDIIDIDLTLHSPED"
     gene            complement(6468..>6718)
                     /locus_tag="VF195_02425"
     CDS             complement(6468..>6718)
                     /locus_tag="VF195_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015470258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX6579710.1"
                     /translation="GVIAGCYMTRGTIRRNSQVRLIRDGVVVYEGRVGSLRRFKDDVA
                     EVREGFECGIGLENFQDVKEGDVIEAFEVEEVARQISS"
BASE COUNT         1091 a         2305 c         2241 g         1081 t
ORIGIN      
        1 tgggtcttca ggtgctcggt cagctccgcg atgcgggtcg tcagtaccgc cacctgcacg
       61 tcggcagagc cggtatcgcc gctggagagg gcgtgctcct ggatgatctg ctgcctggcc
      121 tctttggtca atgtcatgcg aacgtcaagg ctaccagcag ggacgcggtc ggaaccccgg
      181 ccgatcccgc acctaccatg gcttgtgtgt ggagatccga cagtctcagg acggcctgcg
      241 ggatcctcgg gcccgcggcg ttcacaggag cctggcttct tgcccagcag cggcaggagg
      301 aataccaggt gcggcacgag cacatcagcg gcctcgcggc ccgcgacgcc accgatccat
      361 atctgatgac ggcggggttc ctggcgctcg gctcgtgcac ggtcgcgttc gcctcctcgc
      421 tggaggaacg tctcggcccg cgacccggac tcgggccgac gctgatcgga gtggcggggc
      481 tcgccacgat ctccgcgggg ctcttccggc gcgatcggcg atcgaactac ccaccgcccg
      541 gcgtgcccga tggtcagtcc tgggtgaacg acatgcacga ccttgccgcc gcggtgggcg
      601 gagcagccgg gacggccggg ctggtggcgc tcgccccggc gctcgcccgg gacccggcac
      661 tccgtgatct ggcgagaccc gcgatcggag cggccggcgc gagtgccggg atctcggcgt
      721 ggttcgtccg cgacgtggtc cgacccggca atgggctgct ccaacgcgcg aacgtcacga
      781 tccccttggt attcatggcc cgcctcgcaa tccgattgat gaaggatgcc cgggaatgag
      841 ccggacctca cgtgccctac ggtggatacg ggacgcgctg agacgcgagg accagtaccc
      901 gcacgatcgc gagagacaat ccaggagggt cgtcttcagg gttggccgcc cgggcccaaa
      961 cgttgagacc gcgagtgctc caggcccagc tctcatcgat ccgatagacg gctactccat
     1021 ggatccgtgt ggttacgggg acatcctccg cggagatgaa cgcaccgatg ggttggacct
     1081 gtccgtgatt gagtgggtcc gtgccccaga agttgaacgt cactgggccc cccgcaaccg
     1141 ggacctcgaa cacggttccc gtatcgtcca cgttcggata tcccgcctcg ccaaggacat
     1201 ggcgagccca tgatgcctct gggcacagct ccatccaagg gccgtaatcg caggtacccg
     1261 gcatcgccgt cccggttacg ggctcgtggc cacgcctcca ggacggacag cagagctcgc
     1321 cggctgtctc ggtgatgacc cgtgtgtgtc cggagacggg gtcgatgaca gagatcgcgg
     1381 ggccgatctc agggcccgga gatgaggagt aggcgaggag agcgccgtcg ggcgaccact
     1441 cggggtcgag cggtccgtcc ccgtcgcccg caagcagctt ccggacgtgc gatccgtcgc
     1501 tcgccatcag atagatccct ggttcgtctc gatcgctcac gaaggcgatc tggcggccgt
     1561 cgggcgacca tgcgggcgaa ccatcgaagc cgggggctct cgtcagtctg gaaacgtgcg
     1621 atccatctgc gttcatgacg tagatctccg ggctggcggt tcgattcgac tcgaaggcaa
     1681 gcttcgttcc gtcaggtgac cacgtcgggt tgaggtcgaa gtccgggtcg tgcgtgagcc
     1741 ggacagggtt cgacccatcg gcgttcatga gccatatatc gtggttggga ccggacgtgt
     1801 ggacgtaggc gatcctcgag cccggcgacc atgcgggaag gaagttccac cctggcgtgg
     1861 tcgtcagctt ggagaggttc gatccgttcg acccgtagat ggttgcgaca tagatctcgg
     1921 tatcgccgcc cgattgcaca tcgaacgcca gcttggtgcc gtcaggagac cacgaagggt
     1981 ggaaagcctc tcctgggagg tcgacggcga tccgcatgtc ggacgatccg tccgcctcca
     2041 ccgtaaagag gctccatttc agggtcgcac catcaaacgc aacgtacgcg atcgttcccg
     2101 tcccaagcgg cacaactggt tctcgcacct cgggtctccc acggaaggcg tcgacaacga
     2161 acccgaggcc ggccgcggcg atgagcaacg cgaagatcgc cacgcgcgcg cgcacgcccg
     2221 agtagcgagg gatcatctgc gttggtcggg gttcggctct cgtcggaggc ttctcctcgt
     2281 ggggaactcc agagcatcca gagcactgaa gcgcttcttc agatcaatca tcgtcaacct
     2341 ccaaaaagtg ctcggagccg tttgcggccc gcgctcagat ggacacgcac cgtcgcggcg
     2401 gtagacccaa cgatctgggc gatctcgcgc gacgtgtagc ccccgtagta gtggagcacc
     2461 agtgcggctc tctgcttcgg cggaagtctc gtgagagcgt cgagaagctc gacagcctca
     2521 ccgcctccgc tggggcaggc ggctcgtgat cagcgggtcg tgctatccga gccttcagtt
     2581 cacctgccgc gatccgaaac gccgcgatcc agacccaccg ttcgggtgtg cgcaaggcgc
     2641 caccgcggcc tatggcctgc gcgaacgcct ctgccagcgc gtcatcggcg atcccacggt
     2701 cgcctgtgta ggcgacgagg gcacgccaaa gcctggctga ctgctcccgg tagacgtgtt
     2761 cgagtccatc ggggtcggcc gacgtcacgg acgcatcctg ctccactatc aggaaaggga
     2821 ccacggcaag ccaaaaggtg tagtgggttg tctcgacatt cgtccgcctg agaccgacga
     2881 gcgatcctcc gcgacgcagg gcgtcggagc tcgtcctgac cgatcgatcc ctcaagggga
     2941 gctgtcgtct gctcctcggt cagatagatc caaccactga gcgggtgcgt tcgacatctt
     3001 cagcgatcgc cgcgatcagc tcatggaccg aatcgaactt cacctcgtcc cgcagtcgct
     3061 cccagaactc cacggtgagc gtgtcgcctc ggagctcgtc gtcggggaaa tccaacagga
     3121 atgcctcgac gtgaaggggc tcggtcccga aggtcggatt cgtgccgaca tcgatcgcgg
     3181 cgaggtggcg accgccgtcc tgcaactccg cgacgcccgc gtagatcccc tggcctggca
     3241 gcaggagccg cggccaggtt cggaggttgg ccgtcgggta gccgagtccc ttccctctac
     3301 cggctccggc tacgacctcc ccgtcgaccg catacctcct gccgagcgcc ctgcgcggcc
     3361 acgcgaggtc gcccgacgcc agggcctccc ggatcgacga ggacgagacg actcggccat
     3421 cgagctcgaa cagatcgacc tcgtccgcgg tcatgctcaa cggttcgccg acttcacgaa
     3481 gggttttgac cgtgcccgcc gcgcggtgtc cgaacgtgaa gttcgccccg actcgggcgt
     3541 ggatcgcgtg caatccgccg gccaggacat cacggacgaa gtcctccgcc gatacctgcg
     3601 agaacgccgt cgtgaactcg aggaccacga ggacgtcgac cccacaggcc tcgatcaagg
     3661 acgctttccg gtcgacggtg gtgagcaggc ggggctcctc gcccggtgtc agcgtctcgc
     3721 gcggatgccg gtcgaacgtc gcggccacgc tccgtgcgtc gcgctccttc gcggcctcca
     3781 cggtgcggcg gaacacctcc tgatggccga gatggacgcc gtcgaagaac ccgaccgtca
     3841 cgaccgacgg gccgtcgggg agcggcagcg cctggagacc gcgcaccgta tccatccgcg
     3901 gcaggctacc cggcgccaag ataggcagcg gtcggtcagc ccgccaggat gacgtgcggc
     3961 cgggcgcgcg gtccgtcgtc ttcgtagacc ccgatcagac ggccgttcgg cccgaacacg
     4021 ccgtagggac cggtgaggcc ggcgggaccg agcggcctgc cgtgggaggc ggccgcggcc
     4081 tcgtgggcgt cgagatcgag ccgggggaga tggccgaccg cggcctcgat cgggaacgga
     4141 tcgccggcgg aatccggcgt cgcggcatcc tcgacccggt acgggccaat cgccgtgcgg
     4201 cggagggtcg tcaggtgcgc cccggtcgat acggcggcgc caacgtccgc agcgaggacg
     4261 cggatgtacg tgccaccgga gcagacgacg cggaacgtgg cgtccggggg ctcgaacgag
     4321 atcagctcga acgtctccac gctgatcgga cgcggctccg cctccagtcg ctcccccgcc
     4381 cgcgcggcct cgtagagctt ccggcctccc accttgaccg cgctgtaggc gggcggccgc
     4441 tggagggact cccccacgag cgcgcgcatc gcgacaccga cctgctcctt cgtcacatcg
     4501 accgaggact cacggatcac gtcgccgtcg gcgtccagcg tgtccgtctc gacgccgagc
     4561 cgcagcgtcc cctcgtaggt cttcgcgaga tcacccagga accggaggag ccgggtcgcc
     4621 cgcccgacgc cgatcagcag cagcccggtg gccatcggat cgagcgtccc ggcgtgcccg
     4681 accttcttcg tagcgagggt tcggcgcacg ctgtcgacga cgtcatgcga ggtgatcccc
     4741 gccggcttgt cgacgaggag caggccctcg gcggcggtca cgacggaacg ggcgcttggc
     4801 gcaaggcggc cacgagccgt tcgatcgtcc cctcgggtcc gtgctcggag gtgtagcccg
     4861 ccgcgaggcg atgaccaccg cccccgaacg ccgacgccat ggcggacagg tcgtgcccgc
     4921 cgcgggagcg cacgctcacc ttgaaccgcc catctcgctg ctgcttgagg acggcggcga
     4981 tgtcgacgtc gcgcgcggtg cggagcacgt cgatcacgtc gtcggtctcg cccgccacga
     5041 cgccggcgtc ggcgagatcc gcctgcgtga ggtaggtcca gaccatgctc gcctcgggta
     5101 cttcggtcat gcggccgagc gccgtcccga gcaagcggag gaagcccggc tcgttgtcct
     5161 cgtacatcgc acgcacgagc ctggcgtggt cgaagtcctg ctcgcgaagc cgtgcggcga
     5221 tccggagcgt ctccggggtg gtcgcctggt actggaatcg ccccgtgtcc gtgaccaagc
     5281 ccgcgtagag acagacggcg acgtcccgat cgaagtcgcc acccatcgtc tcgatcagac
     5341 ggaagaccat ctcgcatgtg gatgaggcgt cgggatcgac gagacggacg gtgccgacac
     5401 cgtcgttcga gcgatggtga tcgatccaga tcagctcgcc ggcgcgcgaa gccgccttcc
     5461 ccaggacacc cagccggtcg aacgaggcgc agtcgcacgt caccatgacc ggcgactccg
     5521 cggggaacgc ggcggggggc acgaggcgat cggcaccggg gagcagcgcg atccaccggg
     5581 ggagatcgaa cggctcattc gcgaaggagc acacggtggc gaccccgcga gcgcgcagga
     5641 agttcgagag cccgagcatc gagccgagcg catcggcgtc ggggttcagg tggcaggcga
     5701 gcgccacctc ttcggcggcg gccaggacct ctcccgcccg tcgcagctcg atccgtgcgt
     5761 ccgcgtcagg catcaggctg ctccttctgg tcttctggct gctgatcttc tgggtggcgc
     5821 aggcgctcga tgatctcctc gatgtgctgg tgctgggcga gcgtttgatc cacctggaac
     5881 tcgagctccg gcaggttccg cagccggacc tgcttaccga gcgcctggcg aaggtgcggc
     5941 gtcgccgacc gcagccccgc cgccgtcgaa cgctgctgct tgtcgtcacc caggacggtg
     6001 taggacaccc acgcccgccg caggtccgca gtcacctgga cgccggtcac cgtgacgaag
     6061 ccgatccgag ggtccttgag tttctggatc tcctcggcga ggacctcgcg gaactcttcc
     6121 gccacccgag cggtccgggc tccttgcgtc atctcagcat cccgcctctc tggttcagtc
     6181 ctcgggagaa tggagcgtca gatcgatgtc gatgatgtcg acctcggccc accgctcgac
     6241 gaagcgttcg acgccgtgca tcaccttctt caggtgatgg ccctccgatc cgacggccgc
     6301 cacccccagc tcggcccgct gccacatgtc ctggtatccc gtctccgcga ccgcgaccgg
     6361 gaacgtggac cggagcccgt tcgagagggt cttgatcacg tgacgcttct ccttcaacga
     6421 tccacacgcc gggatccgga ggtcgatccg gagagcggcc accagcatca ggaggagatc
     6481 tggcgggcga cctcctcgac ctcgaaggcc tcgatcacgt cgccctcctt cacgtcctgg
     6541 aagttctcga gcccgatccc gcactcgaag ccttcgcgca cctcggcgac gtcgtccttg
     6601 aaccggcgga gcgatcccac gcggccctcg tagacgacga caccgtcgcg gatcaggcgt
     6661 acctgcgagt ttcggcggat cgttcctcgg gtcatgtaac acccggcgat cacgccga
//
LOCUS       DASUYD010000044        53422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_904504, whole genome shotgun sequence.
ACCESSION   DASUYD010000044 DASUYD010000000
VERSION     DASUYD010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 53422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53422
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904504"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..337)
                     /locus_tag="VF195_02430"
     CDS             complement(143..337)
                     /locus_tag="VF195_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579711.1"
                     /translation="MTDPSSPQVDAAIRAADARAAIAKKITSLAPKVSDDAEAQMVLH
                     LAQAYAALAAEPPRTRGSAS"
     gene            complement(334..1593)
                     /locus_tag="VF195_02435"
     CDS             complement(334..1593)
                     /locus_tag="VF195_02435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6579712.1"
                     /translation="MSQRTQVRRPVVAPLPTEATGTTTSFADLGVSRSVADALRMRGI
                     AEAFPIQSLVLRDAIAGRDVLARSRTGSGKTLAFAVPIVERLTPGGGSPNALILAPTR
                     ELASQVTEEFRAIADVKHLKVAAVYGGVGLGPQAKRARHADIVIATPGRLLDLVQRKM
                     LRLDRVRICVLDEADRMLDMGFLPDVTRILELLRPDRQTMLFSATLDGAVGRLAARFI
                     QNPVRHEVAGHDGPLVTEADHRFVAVDHQGKSRALVRELAADRGLTLVFVRTKRGADR
                     LAKYLKAEGFRVGALHGGMAQGARERALGHFAAGANDVLVATDVAARGLDLEHITHVI
                     NFDPPADETDYLHRVGRTARAGRTGTGITFVTPEKTTDVGKIATVLKLHTEFGDAGLR
                     VESVRRHQSARPRPSSGRRPRPARRGR"
     gene            complement(1771..2748)
                     /locus_tag="VF195_02440"
     CDS             complement(1771..2748)
                     /locus_tag="VF195_02440"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6579713.1"
                     /translation="MRTRARRRCAGLVLVPLVLSLTMCAQIERPGPRGTSPAPLPRIP
                     DGSITVAAAGDIGCPPTDPDFEGEEASECQQHATAALLADVDAVAVLGDLQYPDGTLR
                     TFELGYDLSWGRYADKTYPAAGNHDYHVDGATGYFDYWASKDRPTGEEGSGYYSFDLG
                     SWHLVALNSECDAVPCEEGSEQNEFLERDLMRTSQPCLLAFWHHPLFNSGATHGDSVP
                     SGARAFWDDLTAAGADIVLNGHEHNYQRYGKQTGAGQAASDGIRQFVVGTGGKALYPL
                     LEEKDPNFEAGQANDFGVLKLHLGEGAYSWEFVSIDGTLLDSGGPVPCN"
     gene            2836..3798
                     /locus_tag="VF195_02445"
     CDS             2836..3798
                     /locus_tag="VF195_02445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015102202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03885 family FMN-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6579714.1"
                     /translation="MPTVGIHASHEQLSPTRLLDLARAAEAAGFRAAMCSDHLAPWSE
                     RQGHSGHAWAWLGAAMQATSLPFGVVTAPGQRYHPAVTAQAIATLGDLFPGRFWAALG
                     SGEALNEHVTGDPWPRKEDRDTRLLECVDVIRALLRGEEVTHDGLVRVDRARVWSLPA
                     ELPTLYGAAVSEETARTVASWADGMITVYQPIDVLRRVIDAFREGGGDRAPVAVQVHV
                     SWAEDEETALAIAHDQWRTNVFGSELAWNLELPAQFDEAATRVRPDDVTEAVLVSADL
                     GQHAKWLLELTDLGVDAIYIHHVGKEQDAFIEAFAEHVLPELTS"
     gene            3795..5468
                     /locus_tag="VF195_02450"
     CDS             3795..5468
                     /locus_tag="VF195_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011693911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX6579715.1"
                     /translation="MTTARTSDLWWKNAIIYCLDVETFLDWDGDGTGDLIGLTERIDY
                     LAGIGISCLWLMPFQPSPNRDDGYDITDYYGVDPRLGSLGDFVAMIRTAKDRGMRVII
                     DLVVNHTSDRHPWFRASRSSRTSPFRDWYVWSDEIPEEPKAEVVFPDAEDSIWAWDEK
                     AGQYYLHHFYSHQPDLNIANPRIREEMARIVGFWLELGVDGFRVDAVPFLLETGGIAG
                     DVELDPHDFLRDLRSFMSRRRGDVMMLGEVNLPHKDLQLFFGGPDGDEVQLLFNFILM
                     QSIYLSLARQDARPLAKALRETVRKTDDQQYANFLRNHDELTLDKLTDAQRQEVFDAF
                     GPEEDMQLFGRGLRRRLPSMLDGDEQRIRMVYSLLFSLPGTPVLFYGEEIGMAENLEI
                     EGRLSVRSPMQWTDEKNGGFSSAPPSRLRRPVVEGRFGPLAVNAAEQRRDPDSLLTWM
                     ERLIRRRRETPELGWGAWQVLETDVVGVFAHRCDWNGRSVVAVHNLGADPCIARVDLG
                     EVPEGAKLDDLLDERGVLDEIDGSALELKLGGYGYRWFRIGTPDQRTPP"
     gene            complement(5545..6639)
                     /locus_tag="VF195_02455"
     CDS             complement(5545..6639)
                     /locus_tag="VF195_02455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6579716.1"
                     /translation="MSGIGSTLRRHWVEAAWALFALANVAVIVLLSHWETIPFHVVWV
                     SLTLVYGLRLWRFRTTMVVLLVVSVATGAALAWSIARGHQGPDELAEVPLMAAMFVAM
                     VWHAVRRQAAVEEAGRLAESEHRLLMGQREFVRDASHEFRTPITVARGHAELIRESGP
                     NGQVSKDVDIILEELGHLSRLSERLLLLTTAGHPGFLSPTEVPVEPLLEGLIRRWSPT
                     ADRDWQIRVECRGTVSVDRERLETAIDGLLENAVNATRPGDAIRVGCRAEGETLAIEI
                     ADGGRGIRDEDLPRIFDRFSRVQIDRSRGNGGTGLGLSITKAIVDAHGGSIDADSEEG
                     AGATFRIRLGGFLEDDRARSEVSVVPPMDP"
     gene            complement(6611..7276)
                     /locus_tag="VF195_02460"
     CDS             complement(6611..7276)
                     /locus_tag="VF195_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004989114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579717.1"
                     /translation="MARVLVVDDEPRIVSFVSRALSAEGFQVDGAHDGIQGLELAKTG
                     RYELVVLDLLLPYMNGISVLRDLLESRPDQRVLVLSALSDVETKVQCLEFGASDYLSK
                     PFSLAELIARVRARLRQPSAGPRHRVLRGGALTLDLTRRVVEAEGSQITLSEREFLLL
                     EYLMRQDGEVCSRERLLSEVWGYSFDPGSNLVDVCVGRLRSKLGSDVIETVRNVGYRF
                     NAA"
     gene            7459..7878
                     /locus_tag="VF195_02465"
     CDS             7459..7878
                     /locus_tag="VF195_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6579718.1"
                     /translation="MASRLHRLTVVPAAAALAFVAGSCGGGDGIGATLADFTITLDES
                     SAAAGELTFDVSNDAEQIHEFVIFKTDLAEDQLPTDEDGNVDEAGEGVELIDEIEDIE
                     AGANESLTVTLDAGSYVFLCNIPGHYGQGMHTSLTAS"
     gene            complement(8302..11502)
                     /locus_tag="VF195_02470"
     CDS             complement(8302..11502)
                     /locus_tag="VF195_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579719.1"
                     /translation="MKRCPSCGEENADRARFCQSCARPLGDADAPIADVRKVVTIVFA
                     DVTGSTALGERLDPEALRRVMGRYFDEMAAVIERHGGTVEKFIGDAVMAVFGIPRLHE
                     DDAVRAVRAAIGMREALEVLNQDLEREHGEGLVARIGVNTGEVVAGDPSAGQRLVTGD
                     AVNVAARLEQAAAPGEILLGEPTYRLVKDAVQVEPIDALALKGKEDRVAAFRLVSVAA
                     GVAGHERHLDSPMVGRTKELSLLEHALERAVTERTSHLFTLMGPAGVGKSRLVHEFLT
                     GAKGDASVLRGRCLSYGDGITFYPLAEVIHGAAGIVDSDAPSRARAKLHALLTGAHEA
                     ERVAGPVAGLFGWADPGSTEEAFWAVRKLIEHLARERPVVLVFDDIHWGEPTFLDLLE
                     HLADWTRDAAVLLLCIARPELLEVRPGWGGGKLNATSILLEPLPDDDASTLVDNLLGR
                     ADIPRAARDRILEAAEGNPLFVEEMLAMLIDDGLIRFEDDAWRSVEDLADITVPPTIH
                     LLLAARLDRLDAEERAVMERGAVEGKVFHSGAVSTLSAETVRSGVPTRLLALARKELI
                     RPDRAEFAGEDAFRFRHLLIRDAAYQAMPKEQRSDLHERFAGWLLDAAGERIAEYEEI
                     VGYHLEQAFRYREELGAADDRAREVATRAARHLYASAERADERGDLKAARSLLDRCID
                     LSDGSLRLAALVRQSEILPELGEYTAALETANRAIEIATDVGDRPAALRAELVRILNE
                     ISVDPTHTMAAARRDVEGVLAEAERLGDESLRARAVLALAQVSFFLGRTGDATTMLAE
                     LYDRAKTMARRDRTDVAAQMAICAYFGSVPVEDAFAVLDRSAILRRGGLLGEAHDLRV
                     RAGVLGMAGRFDEAEEMVARSRSIYEELGAPMIMVTTSQIYAETLLLGGRPRDAERVL
                     LEMHEAYEEMGERGFNSTICALLGHALCDQGRFDEAESFAERSRELAAEDDFASQGDW
                     RRVMARVLTQRGSFEDALALADEAVAIMGATDYLDWQGGAHEGRADVLAAAGRGDDAR
                     AAYETALERYGWKGNVVAAARVRTRLDAAGGSVHST"
     gene            complement(11786..12442)
                     /locus_tag="VF195_02475"
     CDS             complement(11786..12442)
                     /locus_tag="VF195_02475"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HEX6579720.1"
                     /translation="MQIDARTEQVVAGSGETRVGHHQAGTAGKRARRLPEMHAVRTHV
                     AGVLRATRVWRRGANAGSGEWLGRLPEGWFVFHDVPLGERGPTVDHLVIGPGGVFTLN
                     TKDLSGAIRVNARSVAHDGRRTSFLAKASAEALRAASLLTAAIDRPVEVRGLLAILAD
                     EWIVKRLPADVYVGGPGSVKHWMLQQPPVLRPSDVIVLVAAAGKPATWTANAGHPRAV
                     "
     gene            complement(12500..12991)
                     /locus_tag="VF195_02480"
     CDS             complement(12500..12991)
                     /locus_tag="VF195_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579721.1"
                     /translation="MSEPSFDPLAGLRALVDHGVRFVLIGGYAAALRGSPMITGDVDV
                     CHARDTENLERMARALDGLHARPRGAPPGVPFRLDAGALAAGDHFTFTTDAGPIDCLA
                     TPAGTDGFADLDTAATDEDLDGLVVRVAALDDLIRMKRAADRPQDRIAVEWLSALRDE
                     LES"
     gene            complement(12988..13302)
                     /locus_tag="VF195_02485"
     CDS             complement(12988..13302)
                     /locus_tag="VF195_02485"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6579722.1"
                     /translation="MRAGTILREARRRAGFTQRELARRTGVPQPAVSRIERDHVSPRL
                     ETLDVLLRGCDLALDLVERPGLGVDRTLIRERLGLSPIERARLAAREWNRTARLRRRP
                     VP"
     gene            complement(13434..14546)
                     /locus_tag="VF195_02490"
     CDS             complement(13434..14546)
                     /locus_tag="VF195_02490"
                     /inference="COORDINATES: protein motif:HMM:NF015551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HEX6579723.1"
                     /translation="MATRSRELRQNLPALVAGLTVAVVFLAVLGIVLAAAGPRGLGLP
                     DRQTSGWIALVYGLPMIPSLVLTLRYRMPLLLTGNVFALIFFVTLGDRVTFAELVGAS
                     MLAGAILLVTAILGLTGQLARWIPVPIVHGMIAGAVMPFVVDVFSALRTSDGEWRVAM
                     MVGAAVVGYLVGQRVLGTRLPPILPAFLAGCLAALLTGQLGEFPSAFSLPAFDVVRPA
                     FSVAAIATVAPVLLALMTVQSNVPSVVYLRDQGYRPPARIINLVCGGGTLLGSLFGPV
                     AVSLALPPVLVTAGPSAGDRLLRYRSIFLPVAAGLVIALLAVVAADLALLIPPVLLLA
                     IAGLALVPALVAAIREISAGPLVLGPCSRSRSRSRT"
     gene            14824..16464
                     /locus_tag="VF195_02495"
     CDS             14824..16464
                     /locus_tag="VF195_02495"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX6579724.1"
                     /translation="MTERSIRRSPSGDPPCRRTAAVLSVLCAVLGASFAASPEAPSPR
                     VERSVLIHEANPSSRLAEDRVLAVGGHITRPLPLIGGFAASVPDDGLPDLAGDPAIAG
                     LSVDARIEMASSDTDVYDVFDPSLVWRKAIRLGQLPSGIDGSGVTVAMIDTGVAHHED
                     LGDRVVARVDFTPGGAGDDQYGHGTHLAGVIAGDGGASGGKWRGVAPGAELVSVKVAG
                     ADGSTDVSVVIAAIQWVVTHRGQYGIRVLNLSFGTDSIQPYAIDPLDAAVERAWAAGI
                     TVVVSAGNRGPGLGTIAKPADDPYVITVGAADLNQTPDRWDDEVAPFSSRGPTQDGFE
                     KPDVVAPGTTIVATRDAGSAVDQLHPDAVVDEHYFKGTGTSQSGAVVSGVAALMYEAN
                     PDLTPRRVKGILMGTAFKTAAYRVGGGDGLIDATGAVLAATSDAANPNFGLVRSTGLG
                     SLDESRGRFHVETDLDGDGVADLVEGELDVLGDPWDSTSWSSTSWSSTSWSSTSWSSL
                     IAENAGWSSTSWSSTSWSGTSWSSTSWSSNSWSSVAWS"
     gene            16480..18681
                     /locus_tag="VF195_02500"
     CDS             16480..18681
                     /locus_tag="VF195_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579725.1"
                     /translation="MQTLPRAVKILIGSAILLGVACVAIRIPSIARWDLADVLAVVVI
                     AGLTIVTERCSIPLRRGSETVNFALTDGVWAGALLLVTPSVLTVAVLLGVGVGQALNR
                     WKPYKIAYNVAQFLVGITLAEVAFTILRSWGDGPTDPILWAGVLGAMYITFAVNATAI
                     MLVVSMVEGSRFLDLFRLPLSLNVVHWLGNMALGILGAILWTAEPVALVLMIAPVALT
                     FTTYRRWVESIRERDQMRDLYEAGRALSAPLASSEDFGGFLGLLEKLLAADRVELVVV
                     DGSSVAVHASAGVRYLTVDEAETSPLEAFVAVHSGVAPQIALVGDEGDVRGVLAVYRP
                     QALSASERLLLDGLANQIAARLQDHRVFGKTLERARLSEIVSHTSDGVFTVDTLGRIT
                     TWNPAMHALTGYTEDEALGRRCHDLLGLDRDTVFSDGAAGDGEARDTEIRTGEGTKKD
                     VRLRTSVIRDDEEAPPARIVVVRDASEETRAEQIKRDFVSMVSHELRTPLTPLKGFLV
                     SLVEGTVEDSAERREEYYRIMLRQAQRLERTVSDMLDVSQIEAGGLVIDLQPVPLDHV
                     LGRLVKEFEEQHPGRRVAVGEIGRPAIVLADPFRVEQVMLNLLSNADKYTAPDLPITV
                     GAIRNGRQVTISVWDKGEGIAPEHLDRIFDRFYRVKDGSGQRLPGTGLGLYIARTLVE
                     AMSGHIWVESEVGVGTAFSFSLPIVEVAPLGEAIPFDVAAAETVDPHLVRR"
     gene            18796..19425
                     /locus_tag="VF195_02505"
     CDS             18796..19425
                     /locus_tag="VF195_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6579726.1"
                     /translation="MRIRNLALTGLAAAAVAYYFDPVKGSARRIRLRGNIRTLTGRAM
                     RRGDRAPLPENVAPVSMPEPTIVEEAPEVRPAEANALPEDAAPVSMPEPPTVGKAPEV
                     RPAEADALPEDESDAAIARRIRTRLEERSDLGTGGLVIDVVRGVAYLRGELDDRQQFD
                     EVVDLTGSVPGIRRVQSLLHLPESERIRRPQSRVISTPTTPPLGDSWNG"
     gene            19901..20716
                     /locus_tag="VF195_02510"
     CDS             19901..20716
                     /locus_tag="VF195_02510"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEX6579727.1"
                     /translation="MSDTVETRSDPGPTDEVASFIAAAERIAVLTGAGISTESGIPDF
                     RGPNGVWTTKPETMKLVHIEDYVRDVEIRVQAWRERMDHPAWIAQPNAGHVALAELER
                     RGSVLALMTQNIDGLHQAAGSEKVLELHGTIWLAKCLSCDRRTRMQQQLERVRAGDPD
                     PPCTECGGIQRSATIAFGEQLDPLVLETAVVASSACDLFLAIGTSLSVQPAASLAAEA
                     HRRGARLVIVNQGETPLDDRADVVLRDPIGEVLPRLVAMSLAQRSRSDGSSSP"
     gene            complement(20680..21129)
                     /locus_tag="VF195_02515"
     CDS             complement(20680..21129)
                     /locus_tag="VF195_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579728.1"
                     /translation="METALLRYAQQRATKWGADRFRAEFHRRDLEERWLVAARTGTTP
                     YTVPADTDTVTETGLSGPVEAGAQENALGFDRIEARMVRPAYARHSSVPRPPAEEPVS
                     GGKRPRRRKRDRSNNDPAWLITPAEASRMSAASRRLYGLDDPSDRDR"
     gene            complement(21184..21414)
                     /locus_tag="VF195_02520"
     CDS             complement(21184..21414)
                     /locus_tag="VF195_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579729.1"
                     /translation="MTVTATAARRILRRQGMPRREIRAVLGATEPLVVRRLLELHRER
                     LEEWHEEQQRSLTSIERSLARERDIVPIGCMD"
     gene            complement(21411..21605)
                     /locus_tag="VF195_02525"
     CDS             complement(21411..21605)
                     /locus_tag="VF195_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579730.1"
                     /translation="MQAKRETEQDWVGPDLPSLSGVVFGSILFMTGGMLMLTVIGAPL
                     GILLFAAGLGWMLTPKDRSR"
     gene            21757..22647
                     /locus_tag="VF195_02530"
     CDS             21757..22647
                     /locus_tag="VF195_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX6579731.1"
                     /translation="MDDPVAELRPLLFSIAYRMLGSASDAEDIVQEAFLRFHRETGAG
                     TEVESPKSYLSAVTTRLCIDHLRSARVRRESYVGTWLPEPLLTEETSDAERHAETADS
                     LSMGFLVLLESLTPVERAVFLLREVFEYDYDEIASVVGKSEDNCRQIAVRARQHVQAG
                     KPRFEASKAEREELARRFFLAAGEGDVESLVGMLAADVVAYADGGGKASAFPRPVHGR
                     DKILRLLQGPAARGERLSVTGIRPAEINGQPGAILLDPDGVPVAAVSLDIADGQVQTL
                     RFISNPEKLEHLRALVTRPG"
     gene            complement(22660..23484)
                     /locus_tag="VF195_02535"
     CDS             complement(22660..23484)
                     /locus_tag="VF195_02535"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6579732.1"
                     /translation="MGYLFDPDNWRWLFSGNNLLFLLRGFWINVQIALVAMVLSLTIG
                     LALALLRVSTRRAVSMVAGVWVDVWRNLPLIFTILYFFLVMPESWRDAYERWVPGWFP
                     TAFQTPFFLAAVIGLTLYNSAVIAEIMRAGIVSLERGQGEAAAAIGLRHWQAMRLVIL
                     PQGLRRMVPATVSQLITLNKDTTLVSIIGIQEVMRHARIVSATSGSPFVGTGVEAPLL
                     QVFLAVGIMFVIVNLTLSRLSRRLELRERRRTGVQVEKVEGLEDQLAIQTALPNER"
     gene            complement(23500..24162)
                     /locus_tag="VF195_02540"
     CDS             complement(23500..24162)
                     /locus_tag="VF195_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6579733.1"
                     /translation="MSELWEVLVRHFPEFRSGFLVTVQLVAVSFAIAIVVGLFVGALR
                     VAPVRWVRWLGGLYVEFFRNVPLLVLLSIAYLGLRRAGIPIGPWVAATASLGLYTAAY
                     VAETIRSGVFAVGKGQIDAGLALGFTYRRVLSQIVIPQAVRSVIPPLGSLIIAMIKNS
                     AIIGAVFALADDLLKEARLIGSDTFQYNEVFFWAAIGYLLLTVSVTFAIRAVERRLVV
                     RR"
     gene            complement(24182..25084)
                     /locus_tag="VF195_02545"
     CDS             complement(24182..25084)
                     /locus_tag="VF195_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6579734.1"
                     /translation="MVRVRWSRRWPVVLVSVFALVAAACGEDEPEPPADDGQVEEFPA
                     GSTMAELQEAGEITIGVKYDVPPFGFKNPQTDAIEGFDVDLGQAIADELGVEPNFIEA
                     ISDNRIPFLVDGTADLILSTMTITTDRDAEIDFSIPYYVAEGRVLAPVDSDITGVEGL
                     AGKTVCTATGSTYEENLKENAPEAKLRLVDTYSECLELIQTGAVDAVSTDDVILTGMI
                     IQDDTLELKGEGYTVEPYGAGIADGDADFKEFVDGVIQAFIDDGRWAATFENWIGQYT
                     GETDPPPMMTLEEALELRPCEETC"
     gene            complement(25152..25898)
                     /locus_tag="VF195_02550"
     CDS             complement(25152..25898)
                     /locus_tag="VF195_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6579735.1"
                     /translation="MTSNGHLIEIDNVSKSFGSHLVLDGIDLAISTGEAVVIAGPSGS
                     GKSTLLRCINGLETIDEGDIRFDGHSVPGAGKDLFRLRAEIGMVFQSFNLFAHKSVLD
                     NITLGPIKVKGMSESDANRLARDLLERVGIPEKADDYPADLSGGQQQRVAIARALAME
                     PKLILFDEPTSALDPEMIREVLDVMRDLAHEGMTMIVVTHEMGFAREVCDRIAFVDEG
                     RIVEEGEPEAFFEGAKSQRAKDFVDKILRH"
     gene            complement(25914..26585)
                     /locus_tag="VF195_02555"
     CDS             complement(25914..26585)
                     /locus_tag="VF195_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579736.1"
                     /translation="MIEGRSVPPSAAAPRSGPTRFVQLIAGLVLFGASLALLVRSELG
                     LDPWDVFHQGLSVATGLSIGVSTIAAGVVVLLLWIPLRQRPGIGTVANVVLVGLSLDA
                     VLLLIPATGDLALRWIYLVTGIVLNGIATGAYIGAGLGPGPRDGLMVGLARGGRSLRV
                     VRTAIEVSVLVVGWLLGGTVGIGTVLFASTIGPIVHLTVPAFSRGPGRDGRADVPEAD
                     PGASH"
     gene            complement(26633..26890)
                     /locus_tag="VF195_02560"
     CDS             complement(26633..26890)
                     /locus_tag="VF195_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579737.1"
                     /translation="MRSDVDDDALHEIGVLANTYAIVDEVLASRDPESGEPRIIARID
                     APSAEAALGALLTVISQTSGHAGLAEGSLNRVLIERESPIR"
     gene            complement(26963..27691)
                     /locus_tag="VF195_02565"
     CDS             complement(26963..27691)
                     /locus_tag="VF195_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579738.1"
                     /translation="MSAAILVAAVALTACTPASSDRPIVPTGRPTAVPTVTSFAELDA
                     AIARWHRTSATITYRTERQRPGLPESVHQCLRAVVEERSGVAAALRYCDPSGIVTLVW
                     DPPRRWRLDVGEAGTTTTAIVVGDDGLICVPGAPSCRARTVDAILDELPFVELIIGAA
                     ETARSAGLSADGPVTMTEDVVAGIPVRCLERRAGASAARWCFADDGALLSLALETEGH
                     APTIIEATRVSDGVEDAPFDPPAG"
     gene            27839..28222
                     /locus_tag="VF195_02570"
     CDS             27839..28222
                     /locus_tag="VF195_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018601190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579739.1"
                     /translation="MDERTTEPGERTTATTTEGMSDGSDREDVLELPESGTEAGPLFA
                     EEQSASYRSRWDEIQARFVDDPRGAVGEADELVSTVIGELESSFHSRRDSLEAGWQQG
                     DDTSTEGLRLALQAYRSFFGRLLST"
     gene            28326..28985
                     /locus_tag="VF195_02575"
     CDS             28326..28985
                     /locus_tag="VF195_02575"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6579740.1"
                     /translation="MDRSDPSYKGQRGYNRFLLAIYDPWVLGFMTRAVWKVPISPYVE
                     RYRRLLGRRHLDVGPGTGYFLEKAAPPDGAEITLLDPNPNVLRKASRRLAAMHPATVE
                     ADVMKPLPVEGPFDSAALSYVLHCLRGPQSNKAVAIRNIADVLTPDGVLFGGTVLGLG
                     ERHRPQARAFLRAANLAGDFDNLGDTAEGLREILEESFETVEVEVVGSSADFTATGPR
                     I"
     gene            29019..29705
                     /locus_tag="VF195_02580"
     CDS             29019..29705
                     /locus_tag="VF195_02580"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HEX6579741.1"
                     /translation="MRWDRVVMGATLIAIGVLFLLDRADVLDAGQVIGDWWPAVIVAF
                     GLVTLAERPVSLLGPVLLVAVGVLLLLVTNDVLDTSAWNVIWPAALIAFGLTVLVRNS
                     RRGDHLVLGEGHDQDLVRTTAFLSGSELATQSNAFRGAVLTAIMGGVELDMRHATLAA
                     DGAEVDATAIMGGVDLIIPRDWRVTVRGTPILGGVSNLAEGSSLPDDAPRLRVDALAI
                     LGGVEIKHDK"
     gene            29784..34715
                     /locus_tag="VF195_02585"
     CDS             29784..34715
                     /locus_tag="VF195_02585"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6579742.1"
                     /translation="MGILGSEASTATLTFVIADVRGYTRFTRERGDAAAATLAKKFAD
                     LSRDAVEARGGRVLELRGDEALAVFESPAQAVRAALEFQATCAEESRSDPDLPLPVGI
                     GIDSGEAVPVEEGYRGRALNMAARLCSGAAAGEVLVTRGVADEADLSPAEAIFAERGP
                     RSFKGFEHDVDVIEASFEQPPITDPLPERRPEAATLPPELDPLTPLVDREHEMRWLRG
                     TWRSVRRGRGRVLFVSGPAQIGKTRLAAEIAAHVRAGGGSVRYAGPGGAGTAMALAAL
                     REASTSTLPTLLVLDDIDVAGPQVADAIAKARPDAEGRPLMILGFLRSVDASPALADL
                     VHAVDERGDGHRALSPLDADGVRDIVRLYVGDAVPDVPLESMVRSSEGVPGRVHEVAS
                     DWARSEATRRLEAAAEYLAAGRRLRATDLEFANNVIGLKLGRLYSVEGRNPRRELGDL
                     CPYKGLASFEENDWASFFGRERLVGELAARTVQVGVLGVVGASGSGKSSLVAAGLLPS
                     LGAGLLPGSDRWIHAVMRPGDRPMAALASALVVVDRSIEDADDPLAAAVSTIEQPARI
                     VLVVDQFEEAFTMCESEAERARFIASLAATGHTSPDRCVVVLTIRSDYYGHIAPYPDL
                     AEILAENHVLIGPMSRDELRRAIELPGRRVGLRVEEALVDALVEEVADEPGGLPLLST
                     ALVELWQARQDGWIRMDAHERTGGVKGAVARLAETAYGQLTGVERDAVRRIMLRLVVS
                     DGETVARRRAFLDEFDPARDAVAQRVIMQLARDRLLTLGDGTVEIAHEALLREWPRLR
                     RWLEEDQQGRQVRQQLGDAARHWGSGGRDASELFRGARLSATLDWASTHPGELNELER
                     DFIADSRRASEHEAERQRRTNRRLRGLLAGVAAFLVIALVAGALALVQRASAQREATR
                     AEAQRLGAQALVADDLDLSLLLAREGVNLDDSLATRSNLLAALLRSPAAIGIARPLPG
                     RLLRVHAIPGTASVVVSNNADEFAVVDTTTRTVTDRFTSAFATVSADGTRIGLVDPDG
                     AVRIREVGSADTQTVGKLPTAAVQFFTIEPTFTPDLAKVVAGYFPGPGEPSRMELFDA
                     TTLRSVGRRGSPDGLSFANAIVSSDGRYVATTMSEGEFGEPVSSIYVWRTEDLSAPVR
                     LIRTEDPAGFAAAFSHDGRSLAIGLVDGSVGIVDLRTGRQRVMNGRHNAAIQGLAFSP
                     DDATLVSAGDDEQILVWDVPTGALRETLTGHNGRVLTPAFSADGRTLSTVSLDGTLMM
                     WDLSGTRRLGRSFPASEGVEGPIQTYLAVAPDGERLAIPSAGGGVVIRDAASLDTVRT
                     IRTGTGDVLTAAFSPDGELLATETHLFATEPGGADRYRVALWDARSGEPLADLTGPPL
                     EVELGPDEAFPNDVEALAFSPDGTLLAGGDDAGHVYLWDAASHRVVGDPIEVPPDPQV
                     EINGVLGVAFSHDGSMLAAAHGQNASVWRLPERELLFTVNVDEGYGRSKAVAFSADDR
                     ILATGGGTGVVRFWEAATGAADGGASGHAVPANACWIESLEFSPDGRTLLTSGCDGSA
                     RLFDASSRQEIGSPLQGPGNESNSAVFSPDGSRIFVAYRSGIGFVWNVTLGAWKEQAC
                     TVAGRNLTRAEWERYLPERPYELVCPGIPSAGA"
     gene            complement(34809..37976)
                     /locus_tag="VF195_02590"
     CDS             complement(34809..37976)
                     /locus_tag="VF195_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579743.1"
                     /translation="MLTCANCGRESPEDFAFCPSCSAPLAPPAPQEVRKTVTVVFCDV
                     TGSTAMGERLDPESLRRVMSRYFAEMRAALERHGGTVEKFIGDAVMAVFGVPAIHEDD
                     ALRAVRAASEMREALEVLNKELERDHGVSLAARIGVNTGEVVAGDHGDTLVTGDAVNV
                     AARLEQAAEPGAVLIGEETLRLVRDAVVAEPVPPLEVKGKTEPLAAFRLVQVTAGVAG
                     VARRLDSPMVGRERELARLGQAFEAAIADRSCQLFTILGTPGVGKSRLVEEFLDSLGE
                     ATVLRGRCLPYGEGITFFPVGEVVKEAARLDDFDAPDEIERKICAVLGTDGPACSTLA
                     QLFAAAEHDSSVEETFWAVRSFLEAVAETAPLALVFDDIHWGEPTFLDLIEHITGWAR
                     EAPILMLCVARPELLEERAGWGRGNNATTISLEPLSDDECDDLIGNLLGRAALPEEAR
                     DRILAAAEGTPLFVEEMLSMLIDDGSLARDGDRWVATGPLVDLRVPPTIQALLAARLE
                     QLTGDERAVIQRAAVCGKQFHVGAVASLLDGGEVQPILMSLVRRDVLRPDRSSLAGQD
                     AFRFRHQLIRDASYEAAPKALRAELHERYADWLESVGEDRVEEFEEILAYHLERAYDL
                     LEELGPMDEAGRQLGLRAATHYAASAHRASDRSDYRAASTLHRHAADLLPEDHPDRPR
                     TLYEVGRASVRSLDPGVALAALDAAVEAAATAGQRSIELMARIDRADIQTLIDPIGFT
                     IDDLRAEVLAAKTELEPSGDDEALTTVWRALATVEWMPCRFDAAREAAIRAVEHARRT
                     GDRSLLMDATMTQVAAELLGSTTPAEGRPLIDALRAEFGQEGVVGHATLVQEGCFEAM
                     GGDFDGARRWIRGSTAVAERFGSDLWLAAGLEFGGHVELMAGDPAAAEHAYRKEYELH
                     QRLGDDAHASTSAGYLAMALCRLGRFDEAEQIATIARAIGAEDDMATQASARCAQALV
                     RSERGEHEAARRLAREAADMYAGAQSPWFQGDTLMVLSEVLRAAGVPEEAAEAASAAL
                     AAYERKGHEPGAASARALIDEVSAS"
     gene            complement(38071..41511)
                     /locus_tag="VF195_02595"
     CDS             complement(38071..41511)
                     /locus_tag="VF195_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6579744.1"
                     /translation="MRTCPSCREENAEKARFCSNCGASLEVAPASSTEERKVVSVLFV
                     DLVGFTARSDDADPEDVRATLRVYHDRLREEIERFGGTVEKFIGDAVMAVFGAPTAHE
                     DDAERAVRAALRITEAIEELNAERPGLDLAVRAAVNTGEAVVALGARPERGEGFVTGD
                     VVNVASRLQGIAPVGGVVVGELTHRATRGPIEYEELEPVAVKGKQGPIPVWSAVGARS
                     RFGVDIEPGTATPLIGREQELALLQQLYRRAAGDRAVQLVTLSGEPGVGKSRLVREFR
                     AFIDDEPDLIVWRQGRCLPYGEGITYWALGEVVKAHAGILENDDPEEAAHKLGQAIAL
                     VVEEDGEHDWIRTRLEPLVGLGGDAAAAAGREESFAAWRSFLEAIASTGPFVLVIEDL
                     HWADPALLAFLEHLADWASGVPLLIVCTARPELYERHPSWGGGTRNHTAVALSPLSPE
                     ETARLIGALLDQAVLPAETQSALMSRAGGNPLYAEEFVRMLVDRGVLVRRGAAWELTA
                     GEDEIPVPETVQALIAARLDTLPAERKSLLQDAAVLGKVFWAGAVAAMGGLEAGVVRE
                     GLHELARKELVRPVRRPSIEGETEYSFWHVLIRDVAYGQIPRAGRAAKHVAAARWIER
                     IAGERVEDSAELLAHHYEQAVDLARSAGEGVTALETEALRFLLLATERALRLDTAKAL
                     AHFDKALALTAPGQPDRLRALLVGTRLTTWGGEIDPSRQRIVLEALDEARAQGDRLAE
                     GEVLSALSRLAWAAGDTGQQFELIDQAVAILEELPPGRELARALTRRASAYGLAGRTA
                     ETLRAVEIALPVVRDVGSDVDMAVLLQFGGESRVYIGEVEEGIRDIREGLRLAMDSGP
                     VGMVCSAHVNLGDMVWLREGPAQGQDLYERGIEFGERRGARLGVRWGRMQSMWTRFDL
                     GRWDELLEIGAELIATEPDPGTQIAVLAEIYRQHVLVRRGLSDGSAYESAILPRALEI
                     GDDQVVVPALRVAALGMLARGDPSGAVAAVEELEARMADRPGSRGWLLDECAHVCRIA
                     GAAGTLERLLEGYKPYMTRDRICVRMGEAALAELRGESEAAVEGYREAAEGWAGFPHP
                     LQHGLALLGAGRCLLDLARSSEAGERLRSASEVFRGLGAAPSVAEADDLLERATARSG
                     "
     gene            complement(41585..42076)
                     /locus_tag="VF195_02600"
     CDS             complement(41585..42076)
                     /locus_tag="VF195_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX6579745.1"
                     /translation="MEMEILDDGGYPVEPFAEVISGLTQASAAHPPAEMLSMFDVLID
                     VGGRDRWDGDPDPRYSFHPLDDVPFIVDAEMIHTVGERIASLVSEGKHVAVNCMSGVN
                     RSGLLVGRALVELGYTPEEAIDAVRRARGPMALSNQHFVRFLMVDCTPRALARRRQLS
                     LPL"
     gene            complement(42115..42378)
                     /locus_tag="VF195_02605"
     CDS             complement(42115..42378)
                     /locus_tag="VF195_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579746.1"
                     /translation="MSEPDGTTTSPDLPIVGLTNNQVTDLGVKLDPEKAARETKAIRD
                     AFLKVLDRPAGASACRSMSSEGFPFHGFPRALGPVTSVPTQVG"
     gene            42552..43163
                     /locus_tag="VF195_02610"
     CDS             42552..43163
                     /locus_tag="VF195_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579747.1"
                     /translation="MGIETRDEYAYTGLHAYVLIRHVVAESKHPERNTRALVDEFREA
                     INVEGPITAVVEAAGAFKVLVHLRFDDSEGHEPVDELQEFLLEDRWDGVEYDLVLEGP
                     AYTGPTNELIGFKRHTSTIVAIIKVWVEHGRAREVLGRLGDELGNAFHGASIVFGDCD
                     ILLALEGDDVKSVAGAALGRLQKLDGIARTETSFTDWRRAEVE"
     gene            complement(43167..46340)
                     /locus_tag="VF195_02615"
     CDS             complement(43167..46340)
                     /locus_tag="VF195_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579748.1"
                     /translation="MLICGDCGTSNPDHARFCSACGAEVDEHPDRGSRREVTILFSDL
                     VGSTSLSRQLDAETLRSILHRYFDAMRAAVERHGGRVEKFIGDAVMAVFGVPMLHEDD
                     ALRAVRAASEMRTSLQELNDELERQWRVRLDARIGIASGEVVTGGASAQATLITGPSV
                     NLAARLEQAAHPGEVLLSDPTLRLVRRDVETEPLGAISLKGFGDQVVVHRLVSSASPT
                     EPRARGRESNLVGRERELDLLIGALERTVAERRCELVTILGQPGIGKSRLVEGFLEVT
                     GSGVKVLEGRCLSYGEGITFWPMFEIVQGASGFLDTDPVPTAARKIIECMPGLDEAEL
                     VAGRIGQLLGLFGGPPAPEETFWAIRLFVEAQAREQPLVLVLEDIHWAEPTFIAAIEH
                     VVERSQDVPILVVCMARDDLIDTYPGWAEPRAHAVVRLEPLGADDASVLVEGLLGEGD
                     VSAEIRDLIVGRSEGVPLYAEEIVSALQDEGYLLLKGGRWVLTPDLSSFELPTTISGL
                     IAARLDRLQPDERVTIERASIVGRDFLATEVAALSPSTDLREVVERLDALATKDLLRR
                     IDVGDDGEWTYRFRHLLILDTAYEAMRKTMRAELHERYANWLEGRVQRQSEKYEELIA
                     YHLERAYALRSDVGPADAHLEELRERAAHRLSGAGRLASGRGDMPASANLLGRAIRLL
                     AVGAPERNAILHDLALALWQAGDIDEVEAVYREELEAGHTSGDPVLEARSRLALAELR
                     METDPASISADELRREAERSIPIFEEAGADNDLADALLILGTTHWLNGAISDMLDVSE
                     RAFSVSRGTQTDRGAASYIGRALVLGTTRCDEALTRLEALVAQVAGARLQEATAALDL
                     ATIYAMVDRPDDAEERAARSLATFEELGQGRWVAEGHHTAGIVLWLSGDAEGAELELG
                     AACEWYEHRGELLELGLSSIDLANVLLDLDRVDDAEAIVDVSASSAARYDVEAQIGWR
                     AARARVLARAGRDDDARMLVAEAADLVTGTELLDLRGWTLLAAADVLARSGDGEEARV
                     VAQRARDSFERKGNRVGVKRADAVLRS"
     gene            complement(46478..47113)
                     /locus_tag="VF195_02620"
     CDS             complement(46478..47113)
                     /locus_tag="VF195_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579749.1"
                     /translation="MARRWAQAPDLIEPIVGYRLWRHETDRINVRFLPLGVQDDVTAW
                     KEWDGAWRRWVTASCRLDSEPDGDVPNERCTCGFYAMKSPNEPELLMMVSLDGARSGS
                     DPQRGIVMGRVHLAGKVIEHDRGYRAERARIVELIPTTEEDAETKTIAIRLGLPLAPA
                     IQTWHVPDMLSPGSQPQPVGRTRPLGPVERLRLRVHRRRFRVIEGGSPTVP"
     gene            complement(47562..50945)
                     /locus_tag="VF195_02625"
     CDS             complement(47562..50945)
                     /locus_tag="VF195_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX6579750.1"
                     /translation="MAGFVRRRFERLHLMGSALVALVLVGPLLVASPAAAVPTGFITR
                     QGTELLLDGNPYRFSGLNIFNANSDGWCGAQMAQGTRLDDALTSMGSGASTFRSWFFQ
                     PLAIDKITRDRDWSAFDHTLQVAAAHGKKVIATLTDQWGECGSDVAGNGYKTADWYVT
                     GYQQVQPGMLVSYRDWVAEVVARYQDDPRIAFWQLINEAEVTPCPPGDLQPYQTLHDW
                     ATDVSGLVKSIDANHLVSLGTIGSGQCGADGPRYKDIHAIPAIDLCEYHDYGAPNAGM
                     PGDEFNGLQLRIDQCDDLGKPLFVGEAGIIPNDVGGTLQHRADAFRVKIDTQFSAGVS
                     GFLAWAWSPQDVPVSTLDNYDIGPGDPSLDALAAPPLIVQIAVGGQHTCALSSEGGVW
                     CWGGNDHGELGDGTQLDRLNPKSVQGLDAGVTAIAAGANHACALTASGAVVCWGFNQA
                     GQLGDGTHQDRLVPTPVPDLLSGVTAIAAGSYHTCAVLAAGGVECWGAANRGQLGYGD
                     PSPPWFTSIDSPTPVDGLSSGAVGLAAGDNHACARIAGGGAKCWGANEDFGQVGDGTA
                     SDRNTASDVVVVGQPISDISPGGQHTCAIVSAGASCWGRNVEGQLGDGTTTSRRTADD
                     VAGLTTEVVDIESGGQYTCAIVDAGAAKCWGRNEAGQLGDGSTTPRMEPVDVSGLSSG
                     VQAISASDNHSCAIVGSGNVKCWGLNFFGELGDGTTIERDTPVDVVWLPPDPDPDDPS
                     GLAQFEANGTTSIPVGATTDGTSVVLKATVSDPDGGSVRLQVEVKPVGTAFDGSGLVT
                     STAVGSGSIGSATVTGLLRATGYHWRARTLDGSDQTSQDWVSFGADAETVADFLVERP
                     IVFSSTRHGSNNANHQEIYSMAPDGSLASVVRLTTNSTVDTDPSLSPDGTKITFESNR
                     DGDYEIFVMDVGGSDVVKLTTNTAVDQAAVWSPDGTKIAFVSNRITKNNYDVWTMNAD
                     GTGVAERLTTVVSTDTTPTWSPDGTKIAFMSTRDGDEEIFVMNANGSGQQPLTANNRR
                     DLSPAWSPGGTRIAFTSNREAVNNLDIYAMNVDGSSVIRLTTKAKDDALPAWSGAGLR
                     IVFQGIDTDNDWEIYRVAASGGSLTKLTTNGRTDVAPGW"
     gene            complement(51054..51437)
                     /locus_tag="VF195_02630"
     CDS             complement(51054..51437)
                     /locus_tag="VF195_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018012923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6579751.1"
                     /translation="MPDEETVNVRYMVDDVDEAIAFYTELLGFELLTNASPAFADVRR
                     DLRLLLAGPTSSAGRPMPDGTKPGPGGWNRIHFIVDDIDTEVARLRDAGARFRNDVIE
                     GPGGKQILLEDPSDNVVELFQPAGR"
     gene            51643..52386
                     /locus_tag="VF195_02635"
     CDS             51643..52386
                     /locus_tag="VF195_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX6579752.1"
                     /translation="MPKTDEYRAALEGLARWEPYLLDHSGLPGPRGNIELGQAAADVG
                     TVERFDAFLRWTPERAPIGSKEEFLAFCGTVGLGKLAAAGSREVLPRLRELASDPRWR
                     VREAVAMALQRVGAVDMPALLREMGSWAKGNDLERRAAAAALCEPALLGKEKDAVAVL
                     TILDRITADLTRSKDRSGDDFRALRKGLAYCWSVAVSAAPDRGRELMERWVRSDDPDV
                     RWVMKQNLAKKRIGVVGAGWVAGMTAELE"
     gene            complement(52433..52951)
                     /locus_tag="VF195_02640"
     CDS             complement(52433..52951)
                     /locus_tag="VF195_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579753.1"
                     /translation="MGDEAAHEGIFGHVAIAFVQGDIGMLRSLTREDVVLTLTGSSPL
                     AGTHHGRDAVAGLAIGLGRYVTCTSHLIRFAHDGDRMTAHRDIAIRGQLHRVEMTLHA
                     SFSFDRSDRISAVRLEPSDPGLFDHVISVELGSETGSASIRIPDLPEDQPVGGSSSPA
                     GDPASPRRRGRR"
     gene            complement(53114..>53422)
                     /locus_tag="VF195_02645"
     CDS             complement(53114..>53422)
                     /locus_tag="VF195_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579754.1"
                     /translation="LWFRNMDAVGSLGDWVTSSFGLGLTIGGLLATITAFVGYLGVGR
                     NVERMVDLGGEVAASGGPPTPQQQAELDRLGAALERHGKLDLLLLFLASTAMATARYW
                     "
BASE COUNT         8269 a        18977 c        18164 g         8012 t
ORIGIN      
        1 gcgatcgtga ggcgtgagtg aaccgagagc cgagcgctgc catgctgacc catgagggcc
       61 ctcccttctc gttcgtggct tcgacacctc gaaccgaagg gcagggcccc ttacatgtca
      121 acgatgttgg tgggaagtag atctagctcg cgctgccgcg ggtgcgcgga ggctcggccg
      181 cgagcgcggc gtacgcctgc gcgaggtgca gcaccatctg cgcctccgcg tcgtccgaga
      241 ccttgggagc cagggaggtg atcttcttcg cgatcgccgc tctcgcgtcg gcggcgcgga
      301 tcgcggcgtc gacctggggg ctgctcgggt cggtcatcgt cctcgcctcg ccgggcgcgg
      361 ccgcctgccg gagctcgggc gggggcgggc gctctggtgc cgccgcacgc tctcgacgcg
      421 aaggcccgcg tcgccgaact cggtgtggag cttgaggacc gtcgcgatct tgccgacgtc
      481 cgtcgtcttc tccggcgtga cgaacgtgat cccggtcccc gtccgccccg cacgtgcggt
      541 tcggccgacg cggtggaggt agtccgtctc gtcggccggc gggtcgaagt tgatcacgtg
      601 cgtgatgtgc tcgaggtcca ggccgcgcgc ggcgacgtcg gtcgccacga gcacgtcgtt
      661 cgcgcccgcg gcgaagtggc cgagggcgcg ctcccgcgcg ccttgcgcca tcccgccgtg
      721 gagcgctccg acccggaagc cctccgcctt gaggtacttc gccagtcgat cggcgcctcg
      781 cttcgtccgc acgaacacga gcgtgagacc gcggtcggcg gcgagctctc gcacgagtgc
      841 gcgcgacttc ccctggtggt ccaccgcgac gaagcggtga tccgcctcgg tgacgagcgg
      901 gccgtcgtga ccggccacct cgtgccggac cgggttctgg atgaatcgtg cggcgagccg
      961 accgaccgcg ccgtcgagcg tcgcggagaa cagcatcgtc tggcggtccg gccggagcag
     1021 ctcgaggatc cgcgtcacgt cgggcaggaa gcccatgtcg agcatccggt cggcctcgtc
     1081 gaggacacag atgcggacac ggtccagccg cagcatcttc cgctggacga ggtccaggag
     1141 gcggcccggc gtggcgatca cgatgtcggc gtggcgagcg cgtttggcct gcgggccgag
     1201 gccaacgccg ccgtacacgg ccgccacctt caggtgcttc acatcggcga tggcacggaa
     1261 ctcctccgtg acctggctcg ccagctcacg cgtgggcgcg aggatcagag cgttcgggct
     1321 cccgccgccg ggcgtcagcc gctcgacgat cggtaccgcg aacgcgagcg tcttcccgga
     1381 gccggtgcgc gagcgggcga ggacgtcacg acctgcgatc gcgtcccgga ggacgagcga
     1441 ctggatcggg aacgcctcgg cgatgccgcg catccgcagg gcgtcggcga cggatcggga
     1501 gacgccgagg tcggcgaacg acgtggtcgt tcccgttgcc tcggttggga gtggcgcgac
     1561 gactgggcgc cgcacctggg tgcgttggga catgacagct tcctctcgga tgcgtgtggg
     1621 gatgaacgac tgctccgaaa ggaagggagt tgaggcggcc ttcaccacgt gggcgcgcat
     1681 acgtgatcgc atgggcgcgt gacctctgca gtgtacgcga atcgggcgct cggagggtgg
     1741 agccgggatc ctgctttcgc gtgacgcgat tcagttgcac gggacggggc cacccgaatc
     1801 gagcagggtc ccgtcgatgg aaacgaactc ccatgaatac gcgccctctc cgaggtggag
     1861 cttcagcaca ccgaaatcgt tcgcttgccc ggcctcgaaa ttgggatcct tctcctcgag
     1921 cagcggatag agagctttcc caccggtgcc gaccacgaac tggcggatcc cgtccgacgc
     1981 cgcctgcccg gctccggtct gcttcccgta tcgctggtag ttgtgctcgt gaccgttcag
     2041 gacgatatcg gcccccgctg ccgtcaggtc gtcccagaac gcgcgtgccc cggacggcac
     2101 cgagtcgccg tgcgtcgccc ccgagttgaa cagcggatgg tgccagaagg cgaggaggca
     2161 cggctgcgac gttcgcatca gatcacgctc gaggaattcg ttctgttctg atccttcctc
     2221 gcatggcacg gcatcgcatt cggagttcag ggccacgagg tgccacgagc cgaggtcgaa
     2281 gctgtagtag cccgagccct cctctcccgt gggtcgatcc ttcgatgccc agtagtcgaa
     2341 gtaccccgtc gcaccgtcga cgtgatagtc gtggttgccg gcggcggggt acgtcttgtc
     2401 cgcgtaccgc ccccagctca ggtcgtagcc gagctcgaac gttcgcagcg ttccatccgg
     2461 atactgcagg tcgccgagga ccgcgacggc atcgacgtcg gcgagcaagg ccgcggtcgc
     2521 gtgctgctga cactcgctcg cctcctcccc ctcgaagtcg gggtcggtcg gcgggcagcc
     2581 gatgtcgccc gcggcggcga ccgtgatcga cccgtcgggg atgcgcggaa ggggggccgg
     2641 cgacgtccct cgcggtccgg ggcgctcgat ctgcgcgcac atcgtcaggg acagcaccag
     2701 agggacgaga acgagacccg cgcatcggcg tcgcgctctc gtcctcaccc tgccgagggt
     2761 agcgtccccc cggcgtccgg gaccatcggt cgccggtcgt gcgtggacgg tgcctcctcc
     2821 gggtacggtc ccagggtgcc cacggtcggg atccacgcat cgcacgagca gctctcgccc
     2881 acccgcctcc tcgacctcgc gcgggccgct gaggcagccg ggttccgggc cgcgatgtgc
     2941 tcggatcacc tcgcaccgtg gagcgagcgt caggggcatt ccgggcacgc ctgggcctgg
     3001 ctcggcgccg cgatgcaagc gacgtcgctc ccgttcgggg tggtgaccgc ccctgggcag
     3061 cgctaccacc cggcggtgac cgcccaggcg atcgccacgc tcggcgacct gttccccggc
     3121 cggttctggg ccgcgctcgg ctcgggcgag gcgctgaacg agcacgtgac cggtgacccc
     3181 tggccccgga aagaggatcg agacacccgg ctcctcgagt gcgtggacgt gatccgggcc
     3241 ctcctccgcg gggaagaggt gacccacgac ggcctcgtcc gagtcgaccg tgcgcgggtg
     3301 tggagcctgc ccgccgagct gcccaccctc tacggtgccg cggtgagcga ggagacggcg
     3361 aggaccgtcg cctcctgggc cgacggcatg atcaccgtgt atcagccgat cgacgtcctc
     3421 cggcgcgtga tcgacgcctt ccgcgaggga ggcggcgatc gcgcgccggt cgccgtgcag
     3481 gtccacgtct cctgggcgga agatgaggag acggctctgg cgatcgcgca cgaccagtgg
     3541 cggacgaacg tgttcgggtc ggagctcgcg tggaacctcg agctgccggc gcagttcgac
     3601 gaggccgcca cccgcgtgcg gcccgacgac gtgacggagg cggtcctggt ctcggccgac
     3661 ctcggccagc acgcgaagtg gctcctcgag ctcacggacc tcggcgtgga cgcgatctac
     3721 atccatcacg tcgggaagga acaggacgcc ttcatcgagg cgttcgccga acacgtcctc
     3781 ccggagctga cgtcgtgacg accgcccgca cgagcgatct gtggtggaag aacgcgatca
     3841 tctactgcct cgacgtcgag acgttcctcg attgggacgg ggacggtacc ggcgacctga
     3901 tcggcctcac cgagcggatc gactacctcg ctgggatcgg gatcagctgt ctgtggctga
     3961 tgcccttcca gccctcgccg aaccgagacg acggctacga catcacggac tactacgggg
     4021 tcgacccgcg cctcggctcg ctcggcgact tcgtcgcgat gatccgcacc gcgaaggacc
     4081 gggggatgcg agtgatcatc gacctcgtcg tgaaccacac gtccgaccgc cacccgtggt
     4141 tccgtgcatc gcgttcctcg cggacctctc cgttccggga ctggtacgtc tggtccgacg
     4201 agatccccga ggagccgaag gccgaggtcg tcttcccgga cgccgaggac agcatctggg
     4261 cgtgggacga gaaggcgggc cagtactacc tgcaccactt ctactcgcac cagcccgacc
     4321 tgaacatcgc gaaccccagg atccgagaag agatggcgag gatcgtcggc ttctggctcg
     4381 agctcggcgt cgacggcttc cgggtggacg cggtgccgtt cctcctcgag accggcggga
     4441 tcgccggcga tgtggagctc gacccccacg acttcctccg ggacctccgg tcgttcatgt
     4501 cccggcgcag gggcgatgtg atgatgctcg gggaggtgaa cctgccccac aaggatctgc
     4561 agctgttctt cggcggcccg gacggcgacg aggtgcagct gctgttcaac ttcatcctga
     4621 tgcagtcgat ctacctctcg ttggcgcgac aggacgcccg tccgctggcg aaggcactcc
     4681 gtgagacggt gcggaagaca gacgaccagc agtacgcgaa cttcctccgc aaccacgacg
     4741 agctgacgct cgacaagctg accgacgccc agcgccagga ggtcttcgac gcgttcggtc
     4801 cggaggagga catgcagctc ttcgggcgtg ggctccgccg gcgcctcccc tccatgctcg
     4861 acggcgacga gcaacgtatc cggatggtct acagcctgct gttctcgctc ccgggcacgc
     4921 cggtgctgtt ctacggcgag gagatcggca tggcggagaa cctcgagatc gagggacggt
     4981 tgagcgtccg ctcaccgatg cagtggaccg atgagaagaa cggtgggttc tcctcggctc
     5041 cgccctcgcg cctgcgccga ccggtcgtcg aggggaggtt cggaccgctc gcggtgaacg
     5101 ccgcggagca gcggcgcgac cccgattcct tgctgacctg gatggagcgt ctgatcaggc
     5161 gacggcgcga gacgcccgag ctcgggtggg gcgcctggca ggtcctcgag accgacgtcg
     5221 tgggcgtgtt cgcccaccgg tgcgattgga acgggcgcag cgtcgtcgcc gtgcacaacc
     5281 tcggcgccga cccgtgcatc gcgcgcgtcg atctcggcga ggtacccgag ggtgcgaagc
     5341 tcgacgacct ccttgatgag cgcggggtgc tcgacgagat cgacggatcc gcgctcgagc
     5401 tgaagctcgg tgggtacggc taccgctggt tccggatcgg cacgcccgac cagcggaccc
     5461 cgccgtagcc cccgtcgacg gcgcaggctc aatcctcggc ggccacggcc tgctctcgag
     5521 cggtatcgag ccgggcgtgt tcgatcatgg atccatcgga gggacgacgc tgacctccga
     5581 cctcgcccga tcgtcctcga ggaacccgcc cagacggatc cggaaggtcg ccccagcgcc
     5641 ctcctcgctg tccgcatcga tcgaaccgcc gtgcgcgtcg acgatcgcct tcgtgatgga
     5701 caggcccaag cctgttcccc cgttccccct ggaacgatcg atctggacgc gcgagaaccg
     5761 atcgaagatc ctcgggaggt cttcgtcacg gatccctctg cccccgtccg cgatctcgat
     5821 cgcgagggtc tccccttccg cgcggcaccc aacgcggatc gcatcgccgg ggcgcgtcgc
     5881 gttcaccgcg ttctcgagca gcccgtcgat cgccgtctcc agtcgctccc ggtccacgct
     5941 caccgtgccc cggcactcga cccggatctg ccagtcacga tctgcggtcg gggaccatcg
     6001 gcgtatcagc ccttcgagca atggctcgac gggaacctcg gtcggcgaca ggaaccccgg
     6061 atggccggcc gtcgtcagga gcagcagccg ctcggagagt ctcgacaggt gcccgagctc
     6121 ctcgaggatg atgtcgacgt ccttcgagac ctgaccgttc gggccggact cgcggatcag
     6181 ctccgcgtgg ccgcgcgcca ccgtgatcgg cgtccggaac tcgtgggacg cgtcgcgcac
     6241 gaactcccgc tgccccatca ggaggcgatg ctcggactcg gccaacctcc ccgcctcttc
     6301 gaccgccgcc tgtctccgga ccgcgtgcca caccatcgcg acgaacatcg ccgccatcag
     6361 tggaacttcc gcgagctcgt cgggcccctg gtgaccgcgg gcgatcgacc aggcgagcgc
     6421 cgctccggtc gccacgctca cgaccagcag cacgaccatc gtggtgcgga agcgccacag
     6481 ccggaggccg tacacgagcg tcaggctcac ccagacgacg tggaacggga tcgtctccca
     6541 gtggctgagc agcacgatga cggccacgtt ggccagcgcg aacagggccc acgccgcctc
     6601 gacccagtgc ctacgcagcg ttgaaccgat acccgacatt ccgcaccgtc tcgatcacgt
     6661 cgctcccgag cttggatcgg agccgcccga cgcagacgtc caccaggttg ctgcccggat
     6721 cgaacgagta gccccatacc tcggaaagga gacgctcgcg gctgcagacc tcgccgtcct
     6781 gacgcatcag gtattccagg aggaggaact cccgctcgct cagggtgatc tgactgccct
     6841 ccgcctcgac gacgcgacgg gtcagatcga gcgtcagcgc tcccccgcgg aggacccgat
     6901 gccgcggtcc ggcgctcggc tgccggagcc gggcacgcac gcgcgcgatc agctcggcga
     6961 gggagaacgg cttcgagaga tagtccgacg ctccgaactc caagcactgg accttcgtct
     7021 cgacgtcgga gagggcagag aggacgagga cgcgctgatc ggggcggctc tccagcagat
     7081 cccggaggac ggagatcccg ttcatgtatg ggaggagcag gtcgagcacc acgagctcgt
     7141 acctgccggt cttggcgagt tcgagcccct ggatgccgtc gtgggcgcca tccacctgga
     7201 acccctccgc ggagagcgct cgcgagacga agctgacgat cctcggttcg tcgtcgacga
     7261 ccaacaccct ggccacggtc agatggtagg ccccggcgtc agcgcgcgat acccctgaca
     7321 tgaccacggg atgacggttc cctgacggtc gcgtcacgag ctcccggccg ggacgctccc
     7381 cggctacagt ccccgcgtgc cggccagggc tccacgccga cgcgggccgg aggaaagccg
     7441 agagaagggg gggggatcgt ggcttcacgt ctgcaccggc tgacggtcgt accggccgcc
     7501 gcggcgctcg cgttcgtggc cggcagttgc ggcggcggcg acgggatcgg ggcgaccctg
     7561 gccgacttca cgatcacctt ggacgagtcg agcgccgccg ccggcgagct cacgttcgat
     7621 gtctcgaacg acgcggagca gatccacgag ttcgtgatct tcaagaccga cctcgcggag
     7681 gatcagctcc ctacggacga ggacgggaac gtcgacgaag cgggcgaggg cgtcgagctg
     7741 atcgacgaga tcgaggacat cgaggccggc gcgaacgagt cgttgaccgt gacccttgac
     7801 gccgggagct acgtcttcct ctgcaacata cccggccact acggccaggg gatgcacacg
     7861 tcgttaaccg cgtcctaggg ttcggggtct gcgggagccc gacgtaggcc cacaccgcct
     7921 ccgcgcgctc gtcgacggcg acgcccagca cctcggcccc gatcgggaac gcccgtacgt
     7981 cgagcgtgac cgcgtccagg cgatagagcg agccatcgag gtcgcccacc cacacggccc
     8041 cgaggccggc gctcaggtcg gtgaggtcct gaccgacccg ggtgtgacgt ccctcctcgc
     8101 gggacctggt gttcacccgc gcgacggtgc cggccaggtt gtccagcacc cagagatcct
     8161 cgccggacgt gatgacctga tgtccgtgaa cagccggcgg ccagaccacg cccggtggtg
     8221 cccgctgcat cgccggagtg tatgcccccg cctcaccctg aaggaacggc cctccgtgct
     8281 ggggccgcgc ggggagcgag cctaggtcga gtgcaccgac ccgcccgccg catcgagccg
     8341 tgtccggacc cgcgccgccg ccacgacgtt ccccttccac ccgtagcgct cgagcgccgt
     8401 ctcgtaggca gcgcgcgcgt cgtcgccgcg gcccgcggcc gcgagcacgt cggcgcggcc
     8461 ctcgtgggct ccaccctgcc agtcgaggta atcggtcgcg cccatgatcg ccacggcctc
     8521 gtcggccagg gcgagggcgt cctcgaacga tccccgctgg gtgagcaccc tggccatcac
     8581 gcgcctccaa tcgccctgcg acgcgaagtc gtcctcggcg gcgagctcac ggctcctctc
     8641 cgcgaaggac tccgcttcgt cgaagcggcc ttgatcgcag agcgcgtgcc ccagcagggc
     8701 gcagatcgtc gagttgaatc cccgctcgcc catctcctcg tacgcttcgt gcatctccag
     8761 caggacccgc tccgcgtccc gcggccgtcc cccgaggagc agcgtctccg cgtagatctg
     8821 actcgtcgtg accatgatca tgggcgcccc gagctcctcg tagatcgatc tcgatcgcgc
     8881 gaccatctcc tccgcctcgt cgaaccgtcc ggccatcccc agcacgcccg cccggacgcg
     8941 caggtcgtgg gcctcgccga ggaggccgcc ccgtcggagg atggcggagc ggtcgagcac
     9001 ggcgaacgcg tcctcgaccg ggacggatcc gaagtacgcg cagatcgcca tctgcgcggc
     9061 gacgtcggtg cggtcccggc gggccatcgt cttcgcacgg tcgtagagct ccgcgagcat
     9121 cgtcgtcgcg tcgcccgtcc tcccgaggaa gaacgacacc tgcgcgaggg ccaggacggc
     9181 gcgagcccgg aggctctcgt cgcccaaacg ttccgcctcg gcgagcaccc cctcgacgtc
     9241 ccggcgggcg gccgccatcg tatgcgtggg atcgaccgag atctcgttca ggatccggac
     9301 gagctccgcg cggagcgcgg ccggccggtc gcccacatcg gtcgcgatct cgatcgcgcg
     9361 gttcgcggtc tcgagcgcgg ccgtgtactc gccgagctcc ggaaggatct ccgactggcg
     9421 caccagcgcg gccaaccgga gcgacccgtc ggacaggtcg atgcaccgat ccaggagtga
     9481 tcgcgccgcc ttcaggtcgc cgcgttcgtc cgcccgctcc gcggaggcgt acaggtgccg
     9541 ggcggcccgc gtcgccacct cccgagcccg gtcatcggcc gcgcccagct cctcgcggta
     9601 acggaacgcc tgctcgaggt ggtagccgac gatctcctcg tactcggcga tccgctcccc
     9661 ggccgcatcg aggagccagc cggcgaaccg ctcgtgcagg tccgaccgct gttccttcgg
     9721 catcgcttgg tacgccgcat cgcggatcag cagatgccgg aagcggaacg cgtcctcccc
     9781 ggcgaactcg gcgcgatccg gacggatcag ctccttgcga gcgagcgcga gcaaccgcgt
     9841 cggcacgccg gaccggaccg tctcggcgct gagcgtcgag accgcgcccg agtggaacac
     9901 cttgccctcg acggcgccgc gctccatcac cgcgcgctcc tccgcgtcga gccggtcgag
     9961 gcgcgccgcg agcagcagat ggatcgtcgg gggcaccgtg atgtcggcga ggtcctcgac
    10021 cgatcgccat gcgtcgtcct cgaaccggat gagcccgtcg tcgatcagca tcgcgagcat
    10081 ctcctcgacg aacagcgggt tgccctcggc cgcctcgagg atccgatcgc gcgccgcgcg
    10141 ggggatgtcg gcgcggccga gaaggttgtc cacgagcgtc gaggcgtcgt cgtcgggcag
    10201 cggctcgagc aggatcgacg tcgcgttcag cttcccgcca ccccaaccgg gccggacctc
    10261 gagcagctcc gggcgcgcga tgcacaagag caggaccgcc gcgtcgcgcg tccagtccgc
    10321 gaggtgctcg agcaggtcga ggaacgtggg ctcgccccag tggatgtcgt cgaagacgag
    10381 gaccaccggc cgctctcgag cgaggtgctc gatcagcttg cggaccgccc agaacgcctc
    10441 ctccgtggag ccgggatccg cccagccgaa gagcccggcg accgggcccg ccacgcgttc
    10501 ggcttcgtgc gcgccggtca gcagggcgtg cagtttcgcc cgcgccctgg agggcgcgtc
    10561 gctgtcgacg atccccgccg ccccgtggat cacctcggcc aacgggtaga acgtgatccc
    10621 gtccccgtag gagaggcacc tgcctcgcag gacggaggcg tcgcccttgg caccggtgag
    10681 gaactcgtgg acgaggcgcg acttcccgac gccggccggg cccatcaggg tgaacagatg
    10741 tgaggtgcgc tcggtgacgg cgcgctcgag cgcgtgctcc aggagggaga gctccttcgt
    10801 ccggccgacc atcggagagt cgaggtgccg ctcgtgcccc gcgacgccgg cggcgaccga
    10861 gaccagccgg aaggcggcga cccggtcctc cttccctttc aaggcgaggg cgtcgatcgg
    10921 ctcgacctgc accgcgtcct tcaccaggcg ataggtgggc tcgcccagga ggatctcgcc
    10981 cggcgccgcg gcttgctcca gccgggccgc gacgttcacg gcgtcccccg tcaccaggcg
    11041 ctgcccggcc gaggggtcgc ccgcgaccac ctcgcccgtg ttcacgccga tccgcgccac
    11101 cagcccctcg ccgtgctcgc gctccaggtc ctggttcagc acctcgagcg cctctcgcat
    11161 cccgatcgcg gcccgcaccg ctcgcacggc gtcgtcctcg tgcaggcgcg ggatcccgaa
    11221 gaccgccatc acggcgtcgc cgatgaactt ctcgaccgtc ccaccgtggc gctcgatcac
    11281 ggcggccatc tcgtcgaaat agcgccccat cacgcggcgg agagcctccg ggtccagccg
    11341 ctcgccgagc gccgtggatc ccgtgacgtc ggcgaacacg atcgtgacga ccttgcggac
    11401 atcggcgatc ggtgcgtcgg catcgcccag cggcctggca cagctctgac agaagcgggc
    11461 ccggtcggcg ttctcctcac cgcagctggg gcatcgcttc acggagcgag catagaagcc
    11521 gatcgggagc cgggtgcagc acctcgaagg ttcgctgcgc gcaccggcaa cgttctccgc
    11581 gcagacgccc gggggccatc aggagcgggt acacctcgga tcggagggtt ggcagaggcc
    11641 ttccgagagg cgccggggat gccggcgtcc gaggaggagg tcccccggtc ctaccgaacg
    11701 aggtctgctc cggatggagg agacgacaca gagggatggg ccaggaggcc cgggcggccg
    11761 cccgtcgtcc gcccgggcct cgacgtcaga cggcgcgagg gtgtccggcg ttggcggtcc
    11821 acgtggccgg cttcccggcc gcggcgacga gcacgatcac gtcgctcgga cgcaggaccg
    11881 gcggttgctg caacatccag tgcttcacgc ttccggggcc gccgacgtag acatcggcgg
    11941 ggagtcgctt gacgatccat tcgtcggcga ggatcgcgag gagcccccgg acctccaccg
    12001 gccggtcgat cgcagcggtc aggaggctcg ccgcacggag agcctcggcc gacgccttcg
    12061 ccaggaagct ggtgcgacgc ccgtcgtgag cgacgctccg cgcgttcacg cggatcgccc
    12121 cggacaggtc cttcgtgttg agggtgaaga caccgcccgg cccgatcacc agatggtcga
    12181 cggtcgggcc tcgctctccc agggggacat cgtggaacac gaaccatccc tcgggcagac
    12241 ggccgagcca ctcaccggaa ccggcgttcg ctcctcggcg ccacacccgc gtggcgcgaa
    12301 gcacgccggc cacgtgcgtt cggaccgcat gcatctcggg gagccttcga gcgcgcttcc
    12361 ctgccgtccc ggcctgatgg tgacccacac gcgtctcccc ggatcccgcc accacctgct
    12421 ccgtccgcgc gtcgatctgc atgcacagtt cctcggcacg cgcctggccc ggcctgagcg
    12481 ttgacgacac gagtgtcggt cagctctcga gctcgtcgcg gagcgccgag agccactcca
    12541 ccgcgatccg gtcctgcggc cggtccgctg cgcgcttcat gcggatcagg tcgtcgagtg
    12601 cggccacgcg cacgacgagc ccgtcgagat cctcgtccgt ggccgccgta tcgagatccg
    12661 cgaacccgtc cgtgccggcc ggcgtggcca ggcagtcgat cggacccgcg tcggtcgtga
    12721 acgtgaagtg atcgccggcc gcgagggcac ccgcgtccag ccggaacggc acgcctggcg
    12781 gggcaccacg gggccgagca tggaggccgt cgagtgcgcg cgccatccgc tcgaggttct
    12841 cggtgtcgcg cgcgtgacag acatccacat cgcccgtgat catcggtgag ccgcgcagcg
    12901 cggccgcgta tccgccgatc aggacgaacc gcacgccgtg gtccacgagt gcgcggagac
    12961 cggcgagcgg atcgaacgac ggttcgctca cgggacgggc cgacggcgga ggcgcgccgt
    13021 tcgattccac tcccgagcgg cgagccgggc gcgctcgatc ggcgagagcc cgagccgctc
    13081 gcggatcaag gtccggtcca cgccgaggcc gggccgctcg accagatcga gcgccaggtc
    13141 gcagccgcgg agcaagacgt cgagcgtctc gagcctcggc gacacgtgat cgcgctcgat
    13201 ccgggagacc gccggctgag gcacgccggt cctccgagcg agctcgcgct gcgtgaaccc
    13261 cgctcgccgg cgggcttcac gcaggatcgt gccggctctc atatccctac gataacagaa
    13321 acgatatcaa acctacgacg agcggagctc gcgccacccc tcccgctcga ggaagagaga
    13381 gaccgcggtg ccgaggacga gcgaccagaa gaaggagccg aagccgagga tcgtcatgtc
    13441 cgagagcgcg atcgcgaacg cgaacagggc ccgagcacga gcgggccggc gctgatctcc
    13501 cggatcgcgg ccacgagggc cggcaccagg gcgagtccgg cgatcgcgag cagcagcacc
    13561 ggagggatca gcaacgcgag gtcggcggcc acgacggcca acagcgcgat caccagtccc
    13621 gcggcgaccg gcaggaagat cgaccggtag cggaggagcc ggtcgccggc ggacggcccg
    13681 gccgtcacga ggacgggcgg cagcgccagg gagacagcga ccgggccgaa cagcgaaccg
    13741 agcagggtcc cgcccccgca cacgaggttg atgatccgtg ccggcgggcg gtagccctgg
    13801 tcccgcaggt agaccacgga cggcacgttc gactggaccg tcatcagggc gaggagcacc
    13861 ggggcgacgg tggcgatcgc cgccaccgag aacgccggac ggacgacgtc gaaggcgggg
    13921 agcgagaagg ccgacgggaa ctccccgagc tgtcccgtca ggagggcggc gaggcacccg
    13981 gccaggaacg ccggcaggat cggcggcagc cgcgtgccga gcacgcgctg accgacgagg
    14041 tagcccacca ccgcggcccc caccatcatc gcgacccgcc attcgccatc ggaggtgcgg
    14101 agcgccgaga agacgtcgac cacgaacggc atgaccgcgc ccgcgatcat gccgtgcacg
    14161 atcgggacgg gtatccatcg agcgagctgg ccggtgagcc cgaggatcgc cgtgacgagc
    14221 aggatcgccc ctgcgagcat cgacgcgccg accagttcgg cgaacgtcac gcggtccccg
    14281 agcgtgacga agaagatgag cgcgaagacg ttcccggtga gcaggagcgg catccggtac
    14341 cggagcgtga ggacgaggct cgggatcatc gggagcccgt acacgagcgc gatccagccg
    14401 ctcgtctgcc gatccgggag gccgagccca cgggggccgg ccgcggccag gacgatgccg
    14461 aggaccgcga ggaagacgac cgcgacggtg agcccggcga ccagcgcggg caggttctgc
    14521 cgaagctccc gtgatctcgt cgccatcgga tcctccggtg aacggggacg atgcggctcg
    14581 cggtcggcgc catcctcacg cacggaggat ccgctgtcga cggctccacc gtagcctttc
    14641 ggccaaccgg atcctccccc gcggatccgc ccgcatcccc ctggcgcacc acagctgctg
    14701 tgggacggtc gagaccgcga cattcctccc tcagcgcgat accgctccac cgggtcggtc
    14761 ggtcagagtg acctttcgcc cagggccgct ctcggcccag cgggaccagg aaggggacgg
    14821 gggttgaccg agcgctcgat caggaggtcg ccgtccggcg accctccgtg tcgccgcacg
    14881 gccgccgtcc tctccgtcct ctgcgccgtg ctcggcgcct ccttcgccgc ctcaccggag
    14941 gctccgtcgc cccgcgtcga acgctcggtc ttgatccacg aagcgaaccc ctcctcccgc
    15001 ctcgccgagg accgggtcct cgcggtgggc ggccacatca cgcgtccgct ccccctgatc
    15061 ggcgggttcg cggcgtccgt gccggacgac ggtcttccgg acctcgccgg ggatcccgcg
    15121 atcgccgggc tctccgtcga cgcccgcatc gagatggctt cgtccgacac ggatgtctac
    15181 gacgtgttcg atcccagcct cgtgtggcgg aaggcgatcc gtctgggtca gctccccagc
    15241 gggatcgacg gcagcggcgt caccgtggcg atgatcgata cgggtgtcgc ccatcacgag
    15301 gacttgggcg atcgggtcgt tgcgcgtgtg gacttcacgc ctgggggcgc cggagacgat
    15361 cagtacgggc acggcaccca cctcgcgggt gtgatcgccg gcgacggcgg tgcctcgggg
    15421 ggcaagtggc gcggtgtcgc gcccggcgcg gagctcgtct ccgtcaaggt cgccggcgcc
    15481 gatgggtcca ccgacgtgtc ggtcgtgatc gcggccatcc agtgggtcgt cacgcaccgc
    15541 ggccagtacg ggatccgggt gctgaacctc tcgttcggaa ccgactcgat ccagccgtac
    15601 gcgatcgacc cgctcgacgc agcggtcgag cgtgcctggg ccgcgggcat cacggtcgtg
    15661 gtctccgcgg gcaaccgcgg gcccgggctc gggacgatcg ccaagcccgc cgacgatccg
    15721 tacgtgatca cggtcggtgc ggcggacctg aaccagacgc cggatcgttg ggatgacgag
    15781 gtcgccccgt tctcgagtcg aggccccacg caggacggct tcgagaagcc ggacgtggtg
    15841 gcacccggca cgacgatcgt cgccacccgc gacgccggga gcgccgtcga ccagctccat
    15901 cccgatgccg tcgtcgacga gcactacttc aagggcacgg gcacgtcgca gagcggcgcg
    15961 gtcgtctccg gcgtcgccgc cctgatgtac gaggccaacc cggacctcac gccgaggcgc
    16021 gtgaagggca tcctgatggg aacggcgttc aagaccgccg cctaccgcgt ggggggcggc
    16081 gacgggctca tcgacgccac cggggcggtg ctcgccgcca cgagcgacgc cgcgaacccc
    16141 aacttcggcc tcgtccggtc caccggcctg ggctccctgg acgagagtcg cggccgcttc
    16201 cacgttgaga cggatctcga cggcgacggc gtggccgatc tcgtcgaagg ggaactcgac
    16261 gtcctcggcg acccctggga ctccacctcg tggtcgtcca cttcgtggag ctcgacctcc
    16321 tggagctcga cctcgtggtc gtcgttgatc gcggagaacg ccggctggtc cagcacctcg
    16381 tggagctcga cgagctggag cggcacctcc tggagctcca cgtcctggag ctcgaacagt
    16441 tggagctccg tcgcctggag ctgagagggg aacccgacga tgcagacact tccccgcgcc
    16501 gtgaagatcc tgatcggatc ggcgatcctg ctcggtgtcg cgtgtgtcgc gatccggatc
    16561 ccgagcatcg cccggtggga cctcgcggac gtgctcgcgg tcgtcgtgat cgccgggctc
    16621 acgatcgtca ccgaacggtg ctcgatcccc ttgcgtcgcg gatcggagac cgtgaacttc
    16681 gcgctcaccg acggcgtgtg ggcgggcgcc ctcctcctcg tcacgccgtc ggtcctcacc
    16741 gtggccgtcc tgctgggggt cggcgtgggc caggccctga accgctggaa gccgtacaag
    16801 atcgcctaca acgtcgcgca gttcctcgtg gggatcaccc tcgccgaggt cgcgttcacg
    16861 atcctccgct cgtggggaga cggacccacc gatcccatcc tctgggccgg cgtcctcggc
    16921 gcgatgtaca tcacgttcgc ggtgaacgcc accgcgatca tgctcgtggt atcgatggtc
    16981 gaggggtcgc ggttcctcga cctgttccga ctcccgctct cgctcaacgt ggtgcactgg
    17041 ctcggcaaca tggccctggg catcctgggc gcgatcctgt ggacggcgga gcccgtggcg
    17101 ctcgtgctga tgatcgcgcc ggtcgcgctc acgttcacga cctaccggcg gtgggtcgag
    17161 agcatccgcg agcgcgacca gatgcgcgac ctctacgagg cgggccgtgc cctctccgcg
    17221 ccgctcgcct cctcggagga cttcgggggg ttcctcggtc tgctcgagaa gctcctggcc
    17281 gccgaccgcg tggagctcgt ggtcgtggac gggagcagcg tcgcggtcca cgcctccgcg
    17341 ggcgtgcgct acctcacggt ggacgaagcg gagacctctc ctctcgaggc gttcgtggcc
    17401 gtgcactccg gcgtcgcgcc gcagatcgcg ctcgtgggtg acgagggcga cgtgcggggc
    17461 gtgctcgccg tctaccgacc gcaggcgctg tccgcatcgg agcggctcct cctcgacggc
    17521 ttggccaacc agatcgcagc ccggctgcag gaccacaggg tgttcggcaa gacgctcgag
    17581 cgcgcccgcc tgtcggagat cgtgtcgcac acctccgacg gcgtgttcac ggtcgacacc
    17641 ctcgggcgga tcacgacctg gaaccccgcg atgcacgccc tgaccggcta cacggaggac
    17701 gaggcactcg gacgacggtg ccacgacctc ctgggactcg atcgcgacac ggtcttctcc
    17761 gacggtgccg ccggggacgg cgaggcgcgg gatacggaga tccggacagg cgaaggaacg
    17821 aagaaggacg tgcgcctgcg gacgagcgtg atccgcgacg acgaggaggc gccgcccgcg
    17881 cggatcgtcg tcgtgcgcga cgcgagcgag gagacccgcg cagagcagat caagcgcgac
    17941 ttcgtctcga tggtgtcgca cgagctgcgg acaccgttga cgccgttgaa ggggttcctg
    18001 gtgagtctcg tcgaaggcac ggtcgaggac tctgccgaaa ggcgagagga gtactaccgg
    18061 atcatgctcc gccaggcgca gcgcctggaa cggacggtga gcgacatgct cgacgtctcg
    18121 cagatcgagg ccggcggtct ggtcatcgat ctccagccgg tgcccctgga ccacgtgttg
    18181 gggcgcctgg tgaaggagtt cgaggagcag cacccgggcc gtcgcgtcgc cgtcggtgag
    18241 atcgggcggc cggcgatcgt cctggccgac ccgttccggg tggagcaggt gatgttgaac
    18301 ctgctctcga acgccgataa gtacacggct ccggacctgc cgatcacggt cggcgccatc
    18361 aggaacggcc gccaggtcac gatctccgta tgggacaagg gagagggcat cgcaccggag
    18421 cacctcgatc ggatcttcga ccgcttctac cgtgtgaagg acggatccgg gcagcggctc
    18481 cccggcacgg ggctcggcct ctacatcgcg cggaccctgg tcgaggcgat gtcgggacac
    18541 atctgggtcg aatccgaggt cggcgtgggc acggcgttct ccttcagcct gccgatcgtc
    18601 gaggtcgccc cgctcggcga ggcgatcccc ttcgacgtcg cggcggccga gaccgtggat
    18661 ccgcacctgg tccgtcgctg accgcggtcc ggagccgtct ccatccctac cgatgcagta
    18721 ggccgacctg ggcagttgcg cccacggcca cggcgcccaa ggatggaggt acccttcccc
    18781 acccgaggag gcatcatgcg gatccggaac ctggcgctca ccggcttggc ggcggcagcc
    18841 gtcgcgtact acttcgaccc ggtcaagggg agcgcgagac ggatccggct gcgcgggaat
    18901 atccggacgc tcaccggcag ggcgatgcgc cgaggcgacc gggcgccgct ccccgagaac
    18961 gtggcgcccg tatcgatgcc cgaaccgacg atcgtcgagg aggcaccgga ggtgcgtccc
    19021 gcagaggcca acgcgctccc cgaggatgcg gcgcccgtct cgatgcccga accgccgacc
    19081 gtcgggaagg caccggaggt ccgtcccgcc gaggccgacg ccctccctga ggacgagagt
    19141 gacgcggcga tcgcgcggag gatccggacg cggctcgagg agcgctccga cctggggacc
    19201 ggcggcctcg tgatcgatgt ggtgcgcggc gtggcgtacc tccgaggcga actggacgat
    19261 cgtcagcagt tcgacgaggt cgtcgacctc acgggttcgg tgcccggcat ccggcgggtg
    19321 cagagcctcc tccacctgcc ggagagcgag cggatacgtc gcccccagtc cagggtgatc
    19381 agcacgccga ccactccgcc cctcggcgac tcctggaacg gctagcgcgg gctgcaccca
    19441 acgcgcgagt tagcgctgcc cagagctgat tcctctgctt gggagtctca gtggatctac
    19501 gtacgtatcc cgcttcggag ggttcgttcc tgaaagcgag cgggcggccg gcgaggcatc
    19561 gagtgaccgg atcgcgtgga tcgtgggacg ggccatccat gccggtcccg ttgcagggtc
    19621 cgatactcca cccggggccg atcgcggtcc ccgcgcaccc tcgcgaagcg acggatgaac
    19681 ccttggcgtc ggatcgatcc cgtgtgccga ctcgaccgat gcgtacgtat ggcggcgacc
    19741 ggctctgcgg cccggtcgcg agacctcccc agagacggcc gggggtcagg atgccgggag
    19801 ggtcgaacgc ggccatccgc cggcgatgga cccgcttcag cgcagggctg atccgcaaac
    19861 gttcgagtga ccatcgaccc ctggcgctac gctcacgccc gtgtcggaca cagtggagac
    19921 ccgatccgat cccgggccga cggacgaggt cgcgagcttc atcgcggccg ccgagaggat
    19981 cgcggtgctc accggtgcag gcatcagcac cgagtcgggt atccccgact tccgcgggcc
    20041 gaacggtgtg tggaccacga agcccgagac gatgaagctc gtgcacatcg aggattacgt
    20101 gcgcgatgtg gagatcaggg ttcaggcgtg gcgagagcgc atggaccatc ccgcctggat
    20161 cgcgcagccg aacgccggcc acgtcgccct cgcggagctc gaacgccgcg gctcggtgct
    20221 cgccctgatg acccagaaca tcgacgggct ccaccaagcc gccggctccg agaaggtgct
    20281 cgagctccac ggcacgatct ggctcgcgaa gtgcctctcg tgcgaccgcc gcacgcggat
    20341 gcagcagcag ctcgagcgag tgagggccgg cgatcccgac cccccctgca ccgagtgcgg
    20401 cggcatccag aggtcggcga cgatcgcgtt cggcgagcag ctcgacccgc tggtgctcga
    20461 gacggccgtg gtggcctcga gcgcctgcga tctgttcctg gcgatcggga cgtcgctcag
    20521 cgtgcaaccg gccgcatcgc tggcggcgga ggcgcaccgc cggggcgcgc ggctcgtgat
    20581 cgtgaatcag ggcgagacac cgctcgacga ccgcgcggac gttgtcctcc gcgacccgat
    20641 cggcgaggtg ctcccgaggc tggtggcgat gagcttggct cagcgatcgc gatccgacgg
    20701 atcgtcgagt ccgtagagcc gccggctggc ggcggacatc cgcgatgcct cggccggcgt
    20761 gatcaaccac gcgggatcgt tgttcgatcg gtcacgcttg cggcgacgcg gacgcttgcc
    20821 gccggatact ggctcttcgg ccggaggccg cggaaccgac gagtgtcgcg cataggccgg
    20881 ccgcaccatc cgcgcctcga tccggtcgaa gccgagagcg ttctcctgcg cgcccgcttc
    20941 cacgggaccc gacaggccgg tctcggtcac ggtgtccgtg tcggccggaa ccgtgtacgg
    21001 cgtggtgccc gttcgcgcgg ccaccaacca ccgttcctcg aggtcccgcc ggtggaactc
    21061 ggcccggaag cgatccgcgc cccacttggt cgcccgctgc tgcgcgtatc gcagcagggc
    21121 ggtctccacg aggtcctcct cggtcagcac ggagtatcta tcggcatcga ggaggtcaga
    21181 acttcagtcc atgcagccga tcggaacgat gtcccgctcg cgggcgagcg accgctcgat
    21241 gctcgtgagc gacctctgct gctcctcgtg ccattcctcc agccgttccc ggtgaagctc
    21301 caggagccgg cgcacgacga ggggttcggt cgcccccagg acggcgcgga tctcccgccg
    21361 cggcatccct tgccgtcgta ggatccgccg cgccgcagtg gccgtgaccg tcatcgcgac
    21421 cggtccttcg gggtcagcat ccagccgagc ccggccgcga acagcaggat cccgagcggt
    21481 gccccgatca ccgtcagcat gagcatcccg ccggtcatga acaggatcga cccgaacacg
    21541 acaccgctga ggctcgggag atccggcccc acccagtcct gctccgtctc tcgcttcgcc
    21601 tgcatctcgc atcacccctt ccaccacacg agacgagaga cccaccgtcc ccgtgacacg
    21661 ggatcgatcc gaaagagaag tcgcggcgat gtcacggaac gagggcctct ctcgtctcgt
    21721 ggcaagatgg agagacgatc cgcgctggag gcacgcatgg acgaccccgt cgccgagctg
    21781 cggccattgc tgttctcgat cgcctaccgg atgctcggca gcgccagcga cgccgaggac
    21841 atcgtccagg aggcattcct tcggttccac cgcgagaccg gggccggcac cgaggtcgag
    21901 tcgccgaagt cgtatctgtc ggccgtgacc acgcgcctgt gcatcgatca tctgcggtcc
    21961 gcgcgggtcc ggcgcgagtc ctacgtgggg acgtggctcc ccgaaccgct gctcaccgag
    22021 gagacctcgg acgccgagcg tcatgcggag accgccgatt cgctctcgat ggggttcctg
    22081 gtgttgctcg aaagcctcac gccggtcgag cgcgccgtgt tcctgctgcg cgaggtcttc
    22141 gagtacgact acgacgagat cgcttcggtg gttggcaaga gcgaagacaa ctgtcgtcag
    22201 atcgcggtcc gtgcccgcca gcacgtgcag gccggcaagc cgaggttcga ggcatcgaag
    22261 gccgaacggg aagagctcgc gcgccgcttc ttccttgctg ccggcgaggg cgacgtcgag
    22321 agcctcgtcg ggatgctcgc ggccgacgtg gtggcctacg cggacggtgg gggcaaggcc
    22381 agcgcgttcc cgcgccccgt ccacggtcgc gacaagatcc tgcggctcct gcaggggcct
    22441 gccgcgcgtg gcgagcgcct gtcggtcacc ggtatccgcc cggccgagat caacggccaa
    22501 ccgggcgcga tcctcctcga cccggacgga gtgcccgtgg ccgcggtgtc gctcgacatc
    22561 gcggacggcc aggtgcagac gctgcgcttc atcagcaatc ccgagaagct cgaacacctc
    22621 cgagcgctgg tgacccgccc cggctgacga gccaacgcgc tatcgctcgt tcgggagggc
    22681 ggtctggatg gcgagctggt cctcgagccc ttcgaccttc tcgacctgca ccccggtccg
    22741 gcgccgctcg cgcagctcca gacgccggga cagccgcgag agcgtgaggt tcacgatcac
    22801 gaacatgatc cccaccgcca ggaacacctg gaggagcggg gcctccacgc ccgtgcccac
    22861 gaaggggctc ccgctggtcg ccgacacgat ccgggcgtgt cgcatcacct cctggatccc
    22921 gatgatgctc accagcgtcg tgtccttgtt cagcgtgatc agctgcgaca ccgtggccgg
    22981 caccatccgc cggagccctt gcgggaggat cacgagtcgc atcgcctgcc agtggcggag
    23041 cccgatcgcc gcagcggcct ccccctgtcc tcgctccagc gacacgatgc ccgcgcgcat
    23101 gatctcggcg atcaccgccg agttgtagag ggtgaggccg atcaccgcgg ccaggaagaa
    23161 cggagtctgg aacgccgtcg ggaaccaccc gggcacccag cgctcgtacg catcgcgcca
    23221 ggactccggc atcacgagga agaagtacag gatggtgaag atcagcggca ggttgcgcca
    23281 gacgtccacc cacacacccg ccaccatgct cacggccctg cgtgtggaga cccgcagcag
    23341 cgccagcgcg aggccgatcg tgagcgagag caccatcgcg accagcgcga tctggacgtt
    23401 gatccagaat ccgcggagca ggaacaggag gttgttcccc gagaagagcc agcgccagtt
    23461 gtccgggtcg aagagatagc ccatcagcgg ctcaccgccc tacctcctga ccacgagccg
    23521 tcgctcgacg gccctgatcg cgaacgtgac actcaccgtc aagagcaggt agccgatggc
    23581 cgcccagaag aacacctcgt tgtactggaa cgtgtcggag ccgatcagcc gcgcctcctt
    23641 caggaggtcg tcggcgaggg cgaacaccgc gccgatgatc gcggagttct tgatcatcgc
    23701 gatgatcagg ctccccagcg gagggatcac gctgcggacc gcctggggga tcacgatctg
    23761 ggacagcacc cgcctatagg tgaaccccag cgcgaggccg gcgtcgatct gccccttccc
    23821 caccgcgaag acgcccgagc ggatcgtctc ggccacgtag gccgccgtgt agagccccag
    23881 gctcgccgtc gccgccaccc acgggccgat cgggatcccg gcacggcgga gcccgaggta
    23941 ggcgatcgac agcagcacga gcagagggac gttgcggaag aactccacgt acagcccgcc
    24001 gagccatcgg acccagcgga ccggcgccac gcggagggcg cccacgaaca gtcccacgac
    24061 gatcgcgatc gcgaaggaca ccgccacgag ctgcacggtg acgaggaagc ccgagcggaa
    24121 ctccgggaag tgccggacca gcacctccca gagctcgctc atcggctcgc gatcgcctcg
    24181 atcagcaggt ctcctcgcac gggcgcagct cgagcgcctc ctcgagggtc atcatcggcg
    24241 ggggatcggt ctcgccggtg tactgcccga tccaattctc gaaggtcgcg gcccaccggc
    24301 cgtcgtcgat gaacgcctgg atcacgccgt cgacgaactc cttgaagtcc gcgtccccgt
    24361 cggcgatccc ggcgccgtac ggctcgaccg tgtagccctc acccttcagc tccagggtgt
    24421 cgtcctggat gatcatcccg gtgaggatca cgtcgtcggt ggacacggcg tccacggcgc
    24481 cggtctggat cagctcgaga cattcggagt acgtgtccac caggcgcagc ttcgcctccg
    24541 gcgcgttctc cttgaggttc tcctcgtagg tcgagccggt ggccgtgcac acggtcttgc
    24601 ccgccaggcc ttcgaccccc gtgatgtcgg aatccacggg tgcgagcacc cgcccctcgg
    24661 cgacgtagta cgggatcgag aagtcgatct ccgcgtcccg atccgtggtg atcgtcatcg
    24721 tcgagaggat caggtccgcc gtaccgtcca cgaggaacgg gatccggttg tccgagatcg
    24781 cctcgatgaa gttcggctcc acgccgagct cgtccgcgat cgcctgcccg aggtcgacgt
    24841 cgaagccttc gatcgcgtcg gtctgcgggt tcttgaaccc gaacggcggg acgtcgtact
    24901 tcacgccgat cgtgatctcg cccgcttcct gcagctcggc catcgtggac cctgcgggga
    24961 actcctccac ctggccatca tcggccggcg gttcgggctc gtcctcgccg cacgcggccg
    25021 cgacgagcgc gaagaccgag acgagcacca ccggccaccg gcggctccac ctcacacgta
    25081 ccatggctcc tcccttcgag cccgccctcc gcgcgggccg ctccgccccc tctcctgtac
    25141 gtactgattc ctcagtgccg gaggatcttg tccacgaagt ccttggcccg ctggctcttc
    25201 gctccctcga agaacgcctc cggctcgccc tcctccacga tcctcccctc gtcgacgaac
    25261 gcgatccggt cgcacacctc gcgggcgaag cccatctcgt gtgtcacgac gatcatcgtc
    25321 atcccctcgt gcgccaggtc ccgcatcaca tcgagcacct cgcggatcat ctccgggtcg
    25381 agggccgacg tcggctcgtc gaagaggatc agcttcggct ccatcgcgag cgcgcgggcg
    25441 atcgcgacac gctgctgctg gccgcccgag aggtcggccg ggtagtcgtc ggccttctcc
    25501 gggatgccga cccgctccag gaggtcgcgt gccaggcggt tcgcgtcgga ctcggacatg
    25561 cctttcacct tgatcgggcc gagcgtgatg ttgtccagga ccgacttgtg cgcgaacagg
    25621 ttgaacgatt ggaacaccat cccgatctcg gcgcgcagcc ggaagaggtc cttgccggcc
    25681 cccgggaccg agtgcccatc gaaccggatg tcaccctcgt cgatcgtctc gaggccgttg
    25741 atgcagcgca ggagcgtgga cttcccggat cccgacggtc ccgcgatcac gaccgcttcc
    25801 ccggtggaga tcgcgaggtc gatcccgtcg aggaccagat gggagccgaa gctcttgctg
    25861 acgttgtcga tctcgatcag atgcccgttc gaggtcatcg cgtgcggacg agtctagtga
    25921 gacgccccgg ggtccgcttc cggcacgtcc gcccttccat ctcgccccgg gccccgagag
    25981 aacgcgggca cggtcaggtg gacgatcggc ccgatcgtcg atgcgaacag cacggtcccg
    26041 ataccgacgg tccctccgag gagccatccg acgacgagca cggacacctc gatcgccgtc
    26101 cgcacgacgc ggagcgaccg gccgccgcgc gccaagccga ccatcagccc gtcccgagga
    26161 cccgggccga ggccggcacc gatgtaggcg ccggtcgcga tcccgttcaa gacgatgccc
    26221 gtgacgaggt agatccagcg gagcgcgagg tcgcccgttg cagggatcag gagcaggacg
    26281 gcgtcgagcg agagcccgac cagcacgacg ttcgcgacgg tgccgatccc cgggcgctga
    26341 cgcagcggga tccacagcag caggaccacg acacccgcgg cgatcgtgct caccccgatc
    26401 gacagaccgg tggcgaccga cagaccctgg tggaagacgt cccagggatc gaggcccagc
    26461 tcgctcctga ccaggagcgc gaggctggcg ccgaacagca ccaggccggc gatcagctgt
    26521 acgaaccgtg tcggtccgct ccgaggtgcg gcagcactcg gcggaaccga tctcccctcg
    26581 atcatcgcgc cacatcatcg ccgaccacca gcaccgacca cttggatgcg gactagcgga
    26641 tgggcgattc gcgctcgatg aggactctgt tcagagagcc ctccgccaac ccggcatgcc
    26701 cggaggtctg ggagatcacg gtgagcaggg cgccgagcgc cgcttctgcg ctcggggcat
    26761 cgatcctggc gatgatgcga ggctctccgc tctccggatc acgcgaggcc agcacctcgt
    26821 cgacgatcgc gtacgtgttc gcgaggacac cgatctcgtg cagggcgtcg tcgtcgacgt
    26881 ccgacctcac gcgaagatca agccggtacc acatcgcccg cgcaccgtag cgcggcccga
    26941 ggagcccgta cagccggagg cgtcatccgg caggcggatc gaagggagcg tcctcgactc
    27001 catccgagac gcgtgtcgcc tcgatgatcg tcggggcatg gccctcggtc tcgagcgcga
    27061 gcgagagcaa ggctccgtcg tcggcgaagc accagcgagc ggccgacgca ccggctcggc
    27121 gttcgaggca tcggacgggg atgcccgcca cgacgtcctc ggtcatcgtg accgggccgt
    27181 cggccgagag gccggcgctc cgggcggtct cggccgcccc gatgatcagc tccacgaacg
    27241 gaagctcatc gaggatcgca tcgaccgtgc gagcccggca ggacggtgcc ccaggaacgc
    27301 agatgagccc gtcgtccccg acgacgatcg ccgtggtcgt cgttccggcc tctcccacgt
    27361 cgagccgcca gcgccgtgga ggatcccaga cgagggtgac gatgccgctc gggtcgcagt
    27421 accgcagtgc cgccgcgacc ccgctccgct cctcgaccac cgcgcgcaga cattgatgga
    27481 ccgactcggg cagtcctgga cgctgccgct cggttcgata cgtgatcgtc gcggacgtcc
    27541 ggtgccatct cgcgatggcg gcgtccagct ccgcgaacga cgtgaccgtg gggacggccg
    27601 tcggtctccc cgtgggcacg atcggccgat cagaagacgc aggggtgcac gcggtcagcg
    27661 cgaccgccgc gacgaggatc gcggctgaca cggggccgca tcggcgcctc gcctccatcg
    27721 cgggggagcg tagaccgccc ggatccgccg tggaaggcgt ttgcccctcc cctccatcgg
    27781 gcacatctgc agagacgatc ggatcgaggt cggacgacct cagcggaagg agcgcgagat
    27841 ggacgagcga acgaccgagc cgggggaacg gaccaccgcc acgacgactg aaggtatgtc
    27901 cgacgggagc gatcgggagg acgtcctcga gctcccggag agcgggacgg aggccggacc
    27961 gctcttcgcc gaagagcaga gcgcctcgta tcgcagccga tgggacgaga tccaggcccg
    28021 gttcgtggac gacccccggg gcgccgtcgg ggaggccgac gagctcgtga gcaccgtgat
    28081 cggcgagctc gaatcgtcgt tccactcgcg gagggactcc ctggaagccg gctggcagca
    28141 gggcgatgac acgtcgaccg agggtctccg gctcgccctt caggcgtacc gatccttctt
    28201 cggccgcctg ctcagcacct aggatcgatt cgaccccggg tgcaccgggc cctcgcatac
    28261 gatgacctcg atcacctcgc gctggcgttc gcgcgctcgc gcgacgatgt ctcgaggagg
    28321 agaagatgga tcgcagcgac ccctcgtaca agggccagcg cggctacaac cggttcctcc
    28381 tcgcgatcta cgacccctgg gtgcttggtt tcatgacccg cgcggtctgg aaggtcccga
    28441 tctcgccgta cgtcgagcgc tatcgccggc tcctgggacg ccggcacctg gacgtcggcc
    28501 ccggcaccgg ctacttcttg gagaaggccg caccgccgga cggggccgag atcacgctgc
    28561 tcgatccgaa cccgaacgtg ctgaggaagg cgtcacgcag gctcgcggcg atgcacccgg
    28621 ccacggtgga ggcggacgtg atgaaacccc ttccggtcga gggtccgttc gactccgcgg
    28681 cgctcagcta cgtcctccac tgcctgcgtg gaccgcaatc gaacaaggcc gtggcgatcc
    28741 ggaacatcgc cgacgttctc acgcccgacg gcgtgctgtt cggcggcacc gtcctcggcc
    28801 tgggggaacg acaccggccg caggcgcgtg ccttcctgcg cgcggccaac ctggcgggcg
    28861 acttcgacaa cctcggggac accgccgagg gactccgcga aatcctggag gagtcgttcg
    28921 agacggtgga ggtcgaggtg gtggggtcga gcgcggactt caccgccacc ggtccacgga
    28981 tttgaccggg tcgatcccac gggacacgct ttcccgcgat gaggtgggac cgggtggtca
    29041 tgggcgcgac gctgatcgcg atcggcgtgc tcttcctgct cgatcgcgcc gacgtcctcg
    29101 acgccgggca ggtgatcggc gattggtggc cggccgtcat cgtcgccttc gggctggtga
    29161 ccctcgcgga gcgaccggtc tcgctcctgg gaccggtact gctcgtcgcg gtcggggtcc
    29221 tgctcctcct cgtcacgaac gacgtgctcg acacctcggc ctggaacgtg atctggccgg
    29281 ccgcgctgat cgcgttcggg ctcacggtcc tggtccgtaa cagcagacgt ggtgatcacc
    29341 tcgtcctcgg cgagggacac gatcaggacc tcgtccgcac gaccgcgttc ctgagcggct
    29401 cggagctcgc gacgcagtcg aacgccttcc gtggggcggt cctcacggcg atcatgggag
    29461 gcgtggagct cgacatgcgc cacgccacgc tcgccgcaga cggggcggag gtcgatgcca
    29521 ccgcgatcat ggggggcgtg gacctgatca tcccccggga ttggcgtgtc accgttcgag
    29581 ggacgccgat cctcggaggc gtcagcaatc tcgcggaggg atcttcactt cccgacgacg
    29641 caccccgact ccgggtcgac gccctcgcca tcctcggcgg cgtggagatc aagcacgaca
    29701 agtagcacgg gtacaggtca agatcgccgt cttgtccgat cgtaccggcc acgcgatact
    29761 ccccttcccg ggaaggggga cgagtgggga tcctgggatc cgaggcgagc acggccacgc
    29821 tgaccttcgt catcgcggac gtccggggct acacgcggtt cacccgcgaa cgaggcgacg
    29881 ctgcggcggc gacgctcgcg aagaagttcg ccgacctgtc ccgagacgcc gtcgaggctc
    29941 gtggtggccg cgtcctcgaa ttgcgaggcg acgaggccct cgccgtcttc gagtcgccgg
    30001 cccaggcggt gcgggccgcc ctggagttcc aggcgacgtg cgccgaggag agccggtcgg
    30061 atcccgatct ccctttgccc gtagggatcg ggatcgactc gggagaagct gttcccgtcg
    30121 aagagggcta ccgcggacgg gccttgaaca tggccgcccg gctgtgctcg ggcgcggccg
    30181 cgggagaggt cctcgtgacg cgaggggtcg ccgacgaggc agatctgagt cccgcggagg
    30241 ccatcttcgc cgaacggggt ccgcggtcgt tcaagggatt cgaacacgac gtggacgtga
    30301 tcgaggcctc gttcgaacag ccgccgatca cggacccctt gcccgagcgg aggccggagg
    30361 cggcgacgct cccgccggag ctcgatcccc tcacgccgct cgtcgaccgc gagcacgaga
    30421 tgcgctggct gcgcggaacg tggcgctccg tccgccgagg gcgggggcgc gtcctgttcg
    30481 tgtcgggtcc ggcacagatc ggcaagacac gactcgccgc cgagatcgcg gcgcacgttc
    30541 gagcgggagg cgggagcgtt cggtacgccg gacccggagg agctgggacg gcgatggccc
    30601 tcgccgcgct ccgtgaagcg tccacctcaa cgctgcccac gctgctcgtc ctggacgata
    30661 tcgacgtcgc aggtccgcag gtggccgacg cgatcgcgaa ggctcggccc gacgccgaag
    30721 gcaggccgct catgatcctc ggcttcctgc ggagcgtcga tgcgtctccg gcgctcgccg
    30781 atctcgtcca cgccgtggat gaacgaggag atgggcaccg ggcgctctcg cccctcgacg
    30841 ccgacggcgt gcgcgacatc gtccgcctgt acgtcggcga cgcggtcccc gacgtgccat
    30901 tggaatcgat ggttcgatca tcggaaggcg tgcccggacg ggtgcacgag gtggccagcg
    30961 attgggcgag gtcggaagcg acgcggcgac tggaggcggc ggccgagtac ctcgcggccg
    31021 gacgtcgtct ccgggcgacc gatctcgagt tcgccaacaa cgtgatcggg ctgaagctcg
    31081 gcaggctcta ttccgtcgag ggccgcaacc cccgtcggga gctcggcgac ctctgcccgt
    31141 acaaggggct cgcgtccttc gaggagaacg actgggcgag cttcttcggt cgggagcggc
    31201 tcgtgggtga gctcgccgcc aggaccgtgc aggtcggcgt cttgggcgtg gtcggggcgt
    31261 cgggaagcgg caagtcgtcg ctcgtggcgg cgggcctgtt gccgtctctc ggcgccggac
    31321 tgctgccggg cagcgaccgc tggatccatg cggtcatgcg gcccggcgac cgtccgatgg
    31381 ccgccctggc ctccgccctg gtcgtcgtcg accgctcgat cgaggacgcg gacgatccgc
    31441 tcgcggcggc ggtgagcacg atcgagcagc ccgcccggat cgtcctcgtc gtcgaccagt
    31501 tcgaagaggc cttcacgatg tgcgagtcgg aggcggagcg cgcccgcttc atcgcctccc
    31561 tggcagccac ggggcacact tcccccgatc ggtgcgtcgt cgtcctcacg atccggagcg
    31621 actactacgg ccacatcgcc ccgtacccgg atctcgccga gatattggcg gagaaccacg
    31681 tgctgatcgg cccgatgtcg cgcgacgagc tccgtcgggc gatcgagctg ccgggacggc
    31741 gtgtcggcct gcgcgtggag gaggccctcg tcgacgcgct cgtcgaggag gtcgcggatg
    31801 agccgggcgg gctcccgttg ctgtcgacgg cgctcgtgga gctgtggcag gcgcgccagg
    31861 acgggtggat ccgcatggac gcgcacgagc ggacgggcgg cgtcaagggg gcggtggccc
    31921 gtctcgccga gacggcgtac ggacagctga ccggcgtgga gagagacgcg gttcggcgca
    31981 tcatgcttcg cctcgtcgtc tcggatggcg agaccgtcgc gcggcggcga gcgttcctgg
    32041 acgagttcga tccggcgcgc gacgccgtcg cgcaacgggt gatcatgcag ctcgcgcgag
    32101 accggttgct caccctcggc gacggaacgg tcgagatcgc gcacgaggcc ctgctgcggg
    32161 agtggccgcg actgcggcgg tggctggagg aggatcaaca gggacggcag gtccgacagc
    32221 agctcggcga cgcggcgaga cattggggga gcggcggcag agacgcgtcc gagctcttcc
    32281 gtggggcgcg gctctccgcg accctcgatt gggcctcgac gcatcccggc gagctgaacg
    32341 agctggagcg ggatttcatc gcggacagcc gccgggcgag cgagcacgag gccgagcgtc
    32401 agcggcgaac gaaccgtcgg ctccgcggac tgctcgccgg cgttgccgcc ttcctcgtga
    32461 tcgccctcgt cgccggtgcg ctcgcgctcg tgcaacgcgc gtccgcacag cgtgaggcca
    32521 cgcgggcgga ggcgcagcgc ctgggggcac aggccctcgt ggcggacgac ctggacctct
    32581 cgctgctcct cgctcgggag ggcgtgaacc tcgatgactc gctggctacg cggagcaacc
    32641 tgctcgcggc gctcctgagg agcccggcgg cgatcggcat cgcccggccg cttcccgggc
    32701 gtctcctgcg agtgcacgcg atccccggga cggcgtccgt ggtcgtatcg aacaacgccg
    32761 atgagttcgc ggtggtcgat accacgacgc ggacggtgac cgatcgcttc acgtccgcgt
    32821 tcgccaccgt cagcgccgac ggcacgagga tcggcctcgt cgatcccgac ggcgcggtgc
    32881 gcatccggga ggtcgggtcc gccgacacgc agacggtggg gaagcttccg acggccgcag
    32941 tgcagttctt caccatcgag cccacgttca cacccgacct ggcgaaggtg gtcgcgggat
    33001 acttccccgg acccggcgag ccgtcgcgga tggagctctt cgacgcgacc acgctccgtt
    33061 cggtcggccg gcggggcagc cccgacggcc tgagcttcgc gaacgcgatc gtcagctccg
    33121 atggtcggta cgtcgcgacg acgatgagcg agggcgagtt cggggagccg gtcagctcga
    33181 tctacgtctg gcgaacggag gacctctcgg cgccggtgcg cctgatccgg acggaggacc
    33241 cggcgggctt cgcagcggcg ttcagccacg acggccgctc cctcgcgatc gggctcgtcg
    33301 acgggtcggt cgggatcgtc gacctgcgaa ccggtcgaca acgcgtgatg aacggccgtc
    33361 acaacgcggc gatccaggga ctggcgttca gcccggacga cgccacgctg gtgagcgcgg
    33421 gcgacgacga gcagatcctc gtctgggacg tcccgaccgg tgccctgcgg gagacgctca
    33481 ccggccacaa cggccgtgtc ctcactcctg ccttcagcgc cgacggccgc accctctcca
    33541 ccgtcagcct ggacggcacg ctgatgatgt gggacctgtc gggaacccgt cggctcggcc
    33601 gctcgttccc ggcttcggag ggagtcgagg gccccatcca gacgtacctc gcggtcgctc
    33661 ccgacggcga gcggctggcg atcccgtcgg cgggaggcgg cgtcgtgatc cgagacgccg
    33721 cttcgctcga cacggttcgc acgatccgta ccggaaccgg tgacgtgctc acggccgcct
    33781 tcagcccgga tggcgagctc ctggccaccg agacgcacct gttcgcgacg gagccggggg
    33841 gcgccgatcg ataccgggtc gcgttgtggg acgcccgctc gggtgagccg ctggcggacc
    33901 tgaccgggcc cccgctcgag gtggagctcg ggcccgacga agccttcccg aacgacgtcg
    33961 aggcgctggc cttcagcccc gacgggacgt tgctcgcggg cggggacgac gccggccacg
    34021 tctatctctg ggacgccgcg tcgcatcgcg tggtgggtga cccgatcgag gtgccgcccg
    34081 atccacaggt cgagatcaac ggcgtgctgg gcgtcgcgtt cagtcacgac gggtcgatgc
    34141 tcgccgccgc gcatggccag aacgcgtcgg tgtggcggct ccctgagcgg gagctcctgt
    34201 tcaccgtgaa cgtcgacgag ggatacggcc gctccaaggc cgtcgcgttc tccgcggacg
    34261 accggatcct ggcgaccgga ggggggaccg gcgtcgtgcg gttctgggag gcggccacgg
    34321 gagcggcgga cggcggcgcg agcggccacg cggtgccagc caacgcgtgc tggatcgagt
    34381 cgctcgagtt cagccccgac ggccggacgc tcctcacgtc cgggtgcgac ggaagcgccc
    34441 ggctcttcga cgcctcctcg cggcaggaga tcggatcgcc gctccaggga ccggggaacg
    34501 agtcgaactc cgcggtgttc tcacccgacg gcagcaggat cttcgtggcc tatcgcagcg
    34561 gcatcgggtt cgtctggaac gtcactctcg gcgcgtggaa ggaacaggcg tgcacggtcg
    34621 ccggacggaa cctcacccgg gctgagtggg agcgctacct cccggaacgc ccgtacgagc
    34681 tggtctgccc aggcatcccg tcggctgggg cctgaggcgg ctccttgcgc cgcgcccgaa
    34741 gccaccgacc tcgccacgcg ctctcaccgt ccgcacgcgg ctgcccgaga cgccgggcgc
    34801 gcgtgcggtc agctcgccga gacttcgtcg atcaacgccc gggccgacgc cgctcccggc
    34861 tcgtgcccct tgcgctcgta tgcggccaac gccgcgctcg ccgcctcggc cgcttcctcg
    34921 ggcacgccgg ccgcgcggag gacctccgag agcaccatca gggtgtcccc ttggaaccat
    34981 ggactctgcg cacccgcgta catgtctgcg gcctcccgcg cgagccggcg tgccgcttcg
    35041 tgctcgccgc gttcggaccg gaccagtgcc tgggcgcagc gcgcgctcgc ttgcgttgcc
    35101 atgtcgtcct cggcgccgat cgcgcgcgcg atcgtcgcga tctgctccgc ctcgtcgaac
    35161 cgacccagcc gacagagcgc catcgcgagg taccccgcgg acgtggatgc gtgtgcgtcg
    35221 tcaccgaggc gctgatggag ctcgtactcc ttccggtagg cgtgctcggc tgctgccgga
    35281 tcgcccgcca tcaactcgac gtggccgccg aactcgagac ccgctgccaa ccagaggtcc
    35341 gaaccgaacc gctcggcgac cgcggtggat cctcggatcc accgtctcgc gccatcgaag
    35401 tcaccgccca tggcctcgaa gcacccttcc tgaaccaggg tggcgtgtcc gaccacgccc
    35461 tcctgtccga actcagccct cagcgcgtcg atcaacggcc gaccctcggc cggcgtcgtc
    35521 gagccgagca actcggcggc cacttgggtc atcgtcgcgt ccatcagcag cgatctgtct
    35581 cccgtgcgac gagcgtgctc cacggcgcgg atcgccgcct ccctggccgc gtcgaatcgg
    35641 cacggcatcc attccaccgt cgccagcgcc ctccatacgg tcgtcagcgc ctcgtcgtct
    35701 ccgctcggtt cgagctccgt cttcgcggcg agcacctccg cacggaggtc atcgatcgtg
    35761 aacccgatcg ggtcgatcag cgtctggatg tccgcccgat cgatcctcgc catcaactcg
    35821 atcgaccgct gaccagctgt ggcggccgcc tcgaccgctg cgtcgagcgc cgccaacgcc
    35881 acgcctgggt cgaggctgcg cacgctcgcc cgcccgacct cgtacagcgt gcgcggtcta
    35941 tccggatgat cctccgggag gaggtcggcc gcatgccggt gcagggtcga ggccgcccga
    36001 tagtcgctcc gatccgacgc tcggtgggcg ctcgcagcgt agtgcgtggc agctcggagc
    36061 ccgagttggc gccctgcctc gtccatcggc ccgagctcct cgagcaggtc gtatgcccgc
    36121 tcgaggtggt aggcgaggat ctcctcgaac tcctcgacgc ggtcctcccc cacgctctcg
    36181 agccaatcgg cgtaccgctc gtgcagctcg gcgcggagcg ccttgggcgc ggcctcgtac
    36241 gaggcatcgc ggatcagctg gtggcggaac cggaacgcgt cctggcccgc gagcgacgac
    36301 cgatccggcc ggagcacgtc gcgtcggacg aggctcatca ggatcggctg cacctccccg
    36361 ccgtccagca acgacgccac cgcgcccacg tggaactgtt tgccgcacac cgccgcccgc
    36421 tggatcaccg cccgctcgtc gccggtgagc tgttcgagcc gcgcggccaa cagtgcctgg
    36481 atcgtcggag gcacccgcag gtcgaccaac ggtccggtcg ccacccaccg gtcgccgtcg
    36541 cgagccagcg aaccgtcgtc gatcaacatc gagagcatct cctcgacgaa cagcggcgtg
    36601 ccctcggccg cggcgaggat ccgatcgcgc gcctcctcgg ggagcgccgc acgaccgagc
    36661 aggttcccga tcaggtcgtc gcactcatcg tccgagagcg gctcgaggga gatcgtcgtt
    36721 gcgttgttgc cccggcccca acccgcccgc tcttcgagca gctccggccg cgcgacgcag
    36781 agcatcagga tcggggcttc tcgggcccag cccgtgatgt gttcgatcag gtccaggaac
    36841 gtgggctcgc cccagtggat gtcatcgaac accaaggcga gtggtgcggt ctcggcgacg
    36901 gcctccagga acgaccggac cgcccagaac gtctcctcga ccgagctgtc atgctccgcg
    36961 gcagcgaaga gctgcgcgag tgtcgagcag gccggaccgt ccgtcccgag caccgcgcag
    37021 atcttccgct cgatctcgtc cggtgcgtcg aagtcatcca ggcgggccgc ctccttcacg
    37081 acctcgccca cggggaagaa cgtgatcccc tcgccgtacg ggaggcaccg gcctcggagg
    37141 accgtggcct ctccgagcga atcgaggaac tcctcgacca gccgggactt gccgaccccg
    37201 ggggtgccga ggatcgtgaa cagctgacag gagcggtcgg cgatcgcggc ctcgaacgcc
    37261 tgaccgagcc gggcgagctc acgctcccgg ccgaccatgg gcgaatccag gcgccgcgcg
    37321 accccggcga ccccggccgt cacctggacc agacggaacg cggcgagcgg ctccgtcttc
    37381 cccttgacct cgagcggcgg gaccggctcg gcgaccacgg cgtcccggac gaggcggagg
    37441 gtctcctcgc cgatgaggac ggcccccggc tccgcggcct gctccaggcg ggcggcgacg
    37501 ttcacggcgt cgccggtgac gagcgtgtcg ccgtggtcac cggcgacgac ctcgcccgtg
    37561 ttcaccccga tccgggcggc gagcgagacg ccgtggtcgc gctccagctc cttgttgagg
    37621 acctcgaggg cctctcgcat ctcagaggcc gcgcgaacgg cccggagagc atcgtcctcg
    37681 tggatcgccg gaaccccgaa caccgccatc accgcgtcac cgatgaactt ctcgacggtt
    37741 ccgccgtggc gctccagcgc cgcccgcatc tcggcgaagt accgcgacat cacccggcgc
    37801 agtgactcgg gatccaggcg ctcgcccatc gccgtcgagc cggtcacgtc gcagaagacc
    37861 acggtcacgg tcttgcgcac ttcctggggg gcgggtggag cgagtggcgc cgagcacgaa
    37921 gggcagaagg cgaagtcctc cggtgactcc cgcccacagt tcgcgcaggt cagcacgggt
    37981 cgagcctatg ccacgggcct cccgagaacg aatggaagtt cgcctccatc gatcacgacg
    38041 tcggacatcc acagcatgtc gacgggaacc tcagccgctg cgtgcggtcg cccgctccag
    38101 caggtcgtcc gcctcggcga ccgaaggcgc cgcgcccaat ccgcggaaca cctcgctggc
    38161 tgaccgcagg cgctcaccgg cctcgctcga tcgagcgagg tcgagcagac agcgcccggc
    38221 gcccaggagc gcgagaccat gctggagcgg gtgggggaag ccggcccacc cctcggccgc
    38281 ctcgcggtag ccctcgacgg cggcctcgga ctcccctcgg agctcggcca acgccgcctc
    38341 tcccatccgt acgcagatcc gatccctcgt catgtacggc ttgtagccct cgagcaagcg
    38401 ctccagcgtt cccgcggcgc ccgcgatcct gcagacgtgc gcgcactcgt ccagtagcca
    38461 cccgcgcgac cccggtcgat ccgccatcct cgcctcgagc tcttcgaccg cggcgaccgc
    38521 gccggacggg tcgccccggg cgagcatccc gagcgccgcg acccggagcg ccgggaccac
    38581 gacctggtcg tcgccgatct cgagcgcccg cgggaggatc gcggactcgt acgccgaccc
    38641 atccgagaga ccccgccgaa ccagtacgtg ctgccgatag atctcggcga ggacggcgat
    38701 ctgggtcccc gggtcgggct cggtcgctat cagctccgca ccgatctcca ggagctcgtc
    38761 ccagcgcccg aggtcgaacc tcgtccacat cgactgcatc cgaccccacc gaacgcccag
    38821 cctggcacca cggcgctccc cgaactcgat gccgcgctcg tagaggtcct ggccctgggc
    38881 cggcccctcc ctcagccaca ccatgtcgcc taggttcacg tgcgccgagc agaccatccc
    38941 gaccggcccg gagtccatgg cgagacgcag gccctcgcgg atatctcgga tcccttcctc
    39001 cacctcaccg atgtagaccc gggactcgcc gccgaactgg agcagcaccg ccatgtcgac
    39061 gtcggagccg acgtcacgga ccacgggaag cgcgatctcg accgcccgga gcgtctcggc
    39121 ggtccgtccg gcgagcccgt aggccgaggc cctgcgggtg agtgcgcgtg ccagctcacg
    39181 tcccggcggc agctcctcga ggatcgcgac ggcttggtcg atcagttcga actgttgtcc
    39241 cgtgtcgccg gcagcccatg cgaggcgcga cagggcggac aggacctcgc cctcagccag
    39301 ccggtcgccc tgggcccggg cctcgtccag cgcctcgagc acgatccgct gcctggaggg
    39361 atcgatctca ccgccccacg tcgtcaacct ggtcccgacg agcagcgcgc gcaggcgatc
    39421 cggttgccca ggtgcggtga gcgcgagcgc cttgtcgaag tgggcaagcg ccttcgcggt
    39481 gtcgagacgc agggcccgct cggtcgccag cagcaggaag cgcagggctt cggtctcgag
    39541 ggcggtgaca ccctccccag ccgaccgcgc gaggtcgacc gcctgctcgt agtggtgggc
    39601 gagcagctcc gcgctgtcct ccacccgctc cccggcgatc cgctcgatcc acctggccgc
    39661 ggccacatgc ttcgccgcgc gcccagctcg agggatctgc ccgtaggcga cgtcccggat
    39721 caacacgtgc cagaaggagt actcggtctc tccctcgatc gacggccggc gcaccgggcg
    39781 cacgagctcc ttccgggcga gctcgtggag gccctcgcgg acgacgccgg cctcgagacc
    39841 gcccatcgcc gcgaccgcgc cggcccagaa gaccttgccc aggacggccg cgtcctgcag
    39901 cagcgacttg cgctctgccg gcagcgtgtc gagtctcgcg gcgatcaacg cctgcaccgt
    39961 ctccgggacg gggatctcgt cctcgccggc ggtcagctcc caggcggcgc cccggcgaac
    40021 gagcacgccg cggtcgacga gcatccgcac gaactcctcc gcgtacaacg ggttgccgcc
    40081 cgcgcgggac atcagcgcgg actgcgtctc cgctgggagc accgcctggt ccagcagcgc
    40141 gccgatcaga cgcgccgtct cctcgggcga cagcggcgac agcgcgacgg cggtgtggtt
    40201 gcgggttccg ccgccccagg acggatgccg ctcgtacagc tcgggccggg ccgtgcacac
    40261 gatcaggagc ggaacgccgc tcgcccagtc cgccagatgc tcgaggaacg ccaggagcgc
    40321 cggatcggcc cagtgcaggt cctcgatcac gagcacgaac ggtccggtcg acgcgatcgc
    40381 ctcgaggaag ctgcgccagg cggcgaagga ttcctctcgt ccggcggcgg cggcggcgtc
    40441 cccgcccagg ccgaccagcg gctccagccg ggtccggatc cagtcgtgtt cgccgtcctc
    40501 ctcgacgacg agcgcgatgg cctgcccgag cttgtgggcg gcctcctcgg gatcgtcgtt
    40561 ctcgaggatc ccggcatggg ccttcacgac ctccccgagc gcccagtacg tgatgccctc
    40621 gccgtaggga aggcaccgcc cctgtctcca gacgatcagg tccggctcgt cgtcgatgaa
    40681 ggccctgaac tcccggacga gacgcgactt gccgacgccg ggctcgccgg agagcgtgac
    40741 cagctgtact gcccggtcgc ccgccgcgcg acggtagagc tgctgcagca gcgcgagctc
    40801 ctgctcgcgg ccgatcagcg gcgtcgcggt ccccggttcg atgtccacgc cgaaccggct
    40861 gcgggcgccg accgcgctcc agacggggat cggcccctgc ttccccttga cggccaccgg
    40921 ttcgagctcc tcgtactcga tcgggccacg ggtggcgcga tgagtgagct cgccgaccac
    40981 gacgcctccg accggagcga tcccctggag ccgtgacgcg acgttcacga cgtcgccggt
    41041 gacgaagccc tcgccgcgtt cgggtcgagc gccgagcgcg acgacggcct cgccggtgtt
    41101 cacggcggca cggactgcga ggtcgaggcc gggacgctcg gcgttcagct cctcgatcgc
    41161 ctccgtgatg cggagagcgg cccggacggc gcgctcggcg tcgtcctcgt gggctgtcgg
    41221 cgcgccgaac accgccatca cggcgtcgcc gatgaacttc tcgacggtgc caccgaagcg
    41281 ctcgatctcc tcgcggagtc gatcgtgata gacgcgcagg gtcgcgcgga cgtcctcggg
    41341 atcggcgtcg tcagaccgcg ccgtgaagcc gaccaggtcc acgaacagca ccgagacgac
    41401 cttccgctcc tcggtcgagg aggcgggggc gacctcgagc gatgcaccgc agttcgagca
    41461 gaaccgagct ttctcggcgt tctcctcgcg gcagctcgga cacgttcgca tggtcgaatc
    41521 ctagaggtcg cccgcggcga ggtccggccg aggcgtctgc gttgtcgttg accggtcgag
    41581 gaggtcagag cgggagcgac agctgtcggc gtcgtgccag ggctcggggg gtgcagtcga
    41641 ccatgaggaa ccgaacgaag tgctggttgc tgagcgccat cggccctctc gcccggcgca
    41701 ccgcgtcgat cgcctcctcg ggcgtgtagc cgagctccac gagggcgcgt ccgacgagga
    41761 gcccggaccg gttcacgccg ctcatacagt tcaccgcgac gtgcttgccc tcgctgacga
    41821 gggaggcgat ccgctccccc accgtgtgga tcatctccgc gtccacgatg aagggaacgt
    41881 cgtcgagcgg gtggaagcta taccgcggat ccgggtcacc gtcccaccga tcgcgtccgc
    41941 cgacgtcgat caagacatcg aacatcgaca gcatctctgc cggcgggtgc gcggcgctcg
    42001 cttgggtgag ccccgagatc acctcagcga acggctcgac cgggtacccg ccgtcgtcga
    42061 ggatctccat ctccatacag gcatggtcga ctcgacgtcg gcgtccgtca acggtcaccc
    42121 gacctgggtc gggaccgagg tcactgggcc aagcgcccga gggaacccgt ggaacggaaa
    42181 cccttcactg gacatcgacc ggcaggcgga ggccccggca ggccgatcga gcaccttcag
    42241 gaaagcgtca cggatggcct tggtctcccg ggcggccttc tcgggatcga gcttgacacc
    42301 caggtcagtc acctggttgt tggtcagccc cacgatggga aggtcgggcg acgtcgtcgt
    42361 tccatcgggt tcgctcatgg tgtccctcct ctctcgggcg ccggccagcc tggagtagct
    42421 ttctctgcgc ccacccatca acctcccccc gtatccgtgg gcagtcgatc cggttcgtcg
    42481 gaggaggatc aggcgagttg cgttctggtc tcgccaggcg taagacggcg gagagcgaag
    42541 gaggtggcgc catggggatc gagacgcgcg acgagtacgc gtacacgggg ctgcatgcct
    42601 acgtcctgat tcggcacgtc gtcgctgaat cgaagcatcc cgaacgcaac acgagagcgc
    42661 tcgtggacga gttccgcgaa gcgataaacg tggagggacc catcaccgcc gtggtggaag
    42721 cggccggcgc cttcaaggtc ctcgtccatc tgaggttcga tgactccgag ggtcatgagc
    42781 ccgttgacga gctccaggag ttcctcctcg aggaccgatg ggacggcgtc gagtacgacc
    42841 tcgtcctcga aggaccggcc tacaccggtc cgacgaatga gctgatcggc ttcaagcggc
    42901 acacgtcgac gatcgtcgcg atcatcaagg tctgggtgga gcacggacgt gcgcgggagg
    42961 tgctcggccg gctgggcgac gagctcggca acgcgttcca cggcgcgtcg atcgtcttcg
    43021 gcgactgtga catcctcctc gccctcgaag gcgacgacgt gaaatccgtg gccggagcgg
    43081 cgctcggccg gctccagaag ctcgatggca tcgcgcggac cgaaacgtcc ttcacggact
    43141 ggcgccgcgc cgaggtggag tgaggatcac gaccgaagga ccgcgtcggc tcgcttgacg
    43201 ccgaccctat tgcctttccg ctcgaagctg tcgcgtgccc gctgagccac gacgcgcgct
    43261 tcctcgccgt cccccgatcg agcgaggacg tcagcagccg ccaacagcgt ccagccgcga
    43321 aggtcgagga gctcggttcc cgtgacgaga tccgccgctt ccgcaaccag catccgcgca
    43381 tcatcgtcac gccccgccct cgcgagcacc ctcgccctcg cggcgcgcca tccgatctgt
    43441 gcctcgacgt cgtacctggc agcgctcgac gcgctcacgt ccactatcgc ctccgcatcg
    43501 tcgacgcgat ccagatcgag tagcacgttg gcgaggtcga tcgacgagag cccgagctcg
    43561 aggagctcgc cacgatgctc gtaccactcg caggcggccc cgagctcgag ctccgccccc
    43621 tccgcgtcgc cgctcaacca caggacgatc cccgccgtat ggtggccttc ggcgacccag
    43681 cgaccctgcc cgagctcctc gaacgtagcg agcgaccggg ccgcgcgctc ctccgcgtca
    43741 tccggtcggt cgaccatcgc gtagatcgtc gccaggtcca gcgcggcggt ggcctcctgc
    43801 agtcgggcgc cggccacctg cgcgaccagc gcttccagac gcgtgagcgc ctcgtcgcag
    43861 cgggtcgtcc ccagcaccag cgcgcggccg atgtagctcg cggcaccgcg atcggtctga
    43921 gttccccgcg acacggagaa ggctcgctcg gaaacgtcca acatgtcgga gatcgctccg
    43981 ttcaaccagt gcgtcgttcc gaggatcagg agcgcgtcgg ccagatcgtt gtcggccccc
    44041 gcctcctcga agatcgggat gctccgctcg gcctcgcggc ggagctcgtc ggcgctgatc
    44101 gacgcgggat cggtctccat ccggagttcc gcgagcgcca ggcggctccg tgcctcgagg
    44161 accggatccc ctgacgtatg ccccgcttcc aactcctcgc ggtagacggc ctcgacctcg
    44221 tcgatgtcgc ccgcctgcca cagcgcgagc gctaggtcgt gcaggatcgc gttccgttcg
    44281 ggagcaccca ccgcgagcag gcggatcgca cggccgagca gattggcgga ggcaggcatg
    44341 tccccgcgac cggacgcgag ccttcccgcg cccgacagcc ggtgtgccgc ccgctcccgg
    44401 agctcctcca ggtgcgcatc ggccgggccg acatccgatc gcaacgcgta ggcccgttcg
    44461 aggtgatacg cgatcaactc ctcgtacttc tcgctctgtc gttggacgcg gccctcgagc
    44521 cagttcgcgt accgctcgtg gagctccgct cgcatcgtct tcctcatcgc ctcgtacgcg
    44581 gtgtccagga tcagcagatg ccggaagcgg taggtccact cgccgtcgtc gccgacgtcg
    44641 atcctccgca gcagatcctt cgtggccagt gcgtcgagcc gttctaccac ctcacgcagg
    44701 tcggtcgacg gcgacaaggc cgcgacctcc gtcgccagga agtcacgacc gacgatcgac
    44761 gcccgctcga tcgtgacccg ctcgtcgggc tgcaggcggt cgagccgggc agcgatcagg
    44821 ccggagatcg tggtcggcag ttcgaaactg gacaggtccg gggtcaggac ccatctgccg
    44881 cccttcagga ggaggtatcc ctcgtcctgg agggcagaaa cgatctcctc cgcgtacaga
    44941 gggaccccct cggaacggcc gacgatcagg tctcggatct cggccgaaac gtctccttcg
    45001 ccgaggagac cctccacgag caccgacgcg tcgtccgccc cgagcggttc aagcctgacc
    45061 acggcgtgtg cgcgcggctc ggcccaccct ggatacgtgt cgatgagatc gtcccgggcc
    45121 atacacacca ccaggatcgg cacgtcctgc gaccgttcga cgacatgctc gatcgccgcg
    45181 atgaacgtcg gttcggccca gtggatgtcc tcgaggacca agacgagcgg ctgctcgcgc
    45241 gcctgtgcct cgacgaacaa ccggatcgcc cagaacgttt cctcaggcgc cggcgggcca
    45301 ccgaacagac cgagcaactg accgatcctg ccggcgacga gctccgcctc atcgagtccc
    45361 ggcatacatt caatgatctt ccgggcggcg gtcggaaccg ggtcggtatc caggaagccg
    45421 ctcgctccct gcacgatctc gaacatcggc cagaaggtga tgccctcgcc ataggacagg
    45481 caacgccctt cgaggacctt gacgccggag cccgtcacct ccaggaagcc ttcgacgagt
    45541 cgcgacttgc cgatcccggg ctgtccgagg atcgtgacga gctcgcagcg acgctccgcg
    45601 accgttcgct cgagcgcccc gatcaggagg tcgagctctc gctcccgacc gaccaagttg
    45661 gattcccgcc ccctggcccg tggttcggtc ggactggcgg aggagacgag cctatgcacc
    45721 acgacctggt cgccgaaccc cttcagcgag atcgcgccaa gtggctccgt ctccacgtca
    45781 cggcggacta gccggagcgt cgggtcggag aggagaacct ctcccggatg ggcggcctgc
    45841 tccagccgag ccgccaggtt caccgacggc cccgtgatca acgtcgcttg cgcactggcc
    45901 cctcccgtca cgacctcgcc cgacgcgatg ccgatccgcg catccagccg aacgcgccac
    45961 tgtcgttcga gctcgtcgtt gagctcctgg agggatgtcc gcatctccga ggcggcacgg
    46021 acggcgcgga gggcatcgtc ctcgtgcagc atcgggacgc cgaagacagc catgacggcg
    46081 tcgccgatga acttctcgac gcggccccca tggcgttcga ccgcggcgcg catcgcgtcg
    46141 aagtagcggt gcaggatcga ccggagcgtt tccgcatcca gctgccgaga gagcgacgtg
    46201 gaaccgacga ggtcggagaa caggatcgta acctcacgtc gtgagccgcg atccgggtgc
    46261 tcgtcgacct cggcgccgca agcagaacag aacctcgcat gatccgggtt gctcgtcccg
    46321 cagtcgccac agatcagcac gccgacagcg taaggatgcc gcggctccgt cgctagcgaa
    46381 ggtcaccgcc gaggcggaat gccttcgcgg cagggagtgt tctccggcgg tcgcccccgg
    46441 cgaggttccc atcgcctcgg gcggacctct cgcacttcta cggaactgtt gggctgcctc
    46501 cctcgatcac gcggaatcga cgtcggtgga cgcggagacg caaccgctcg acgggaccga
    46561 gaggtcgcgt tcgaccgacc ggttgcggct gcgatcccgg ggagagcatg tcggggacgt
    46621 gccaggtctg gatcgcaggt gccagcggaa gtccaaggcg gatcgcgatc gtcttcgtct
    46681 cggcatcttc ctctgtcgtc gggatgagct cgacgatccg cgcgcgctcg gcccggtacc
    46741 cacgatcgtg ctcgatcacc ttgccggcga ggtggacccg gcccatgacg atccctcgct
    46801 gcggatccga tcccgagcgg gcaccatcga gcgacaccat catgagcagc tcgggttcgt
    46861 tgggtgactt catcgcatag aacccgcacg tgcagcgctc gttcggaacg tcgccatcgg
    46921 gctcgctgtc gagacggcag ctcgccgtca cccacctacg ccaagcgccg tcccactcct
    46981 tccaagccgt cacatcgtcc tggacgccca gggggaggaa tcgaacgttg atgcggtcgg
    47041 tctcgtgtcg ccagaggcga tagccgacga tcggctcgat gagatccgga gcctgcgccc
    47101 acctgcgggc catcgccgtc aacggttggg agcgggttcg ggcagccgtt gaggttcgac
    47161 aggggtcacc ggttcctccc gcaaaggggc gacccttgga gcgcccgggt cctcctcgat
    47221 cggttcgacg tagatcgtcc gacgctgctc gccgatgtcc atcggggaac ctcctgacca
    47281 gccgcactgg ggctcgcgta gctcggccat ggtacgactg ctcgggtggg gcaggccgcc
    47341 tctcggaacg acctgccaac tgtagcgccg ccgccagaca cagtgcgtgt cgacctcgcc
    47401 tgcgcaacaa cgaggcgacc cgtggtgtcg gggatcgtga gcacgtagtc acgttggagt
    47461 cctcggcacc cagcacgacc cgggcgaacc acgccagccg ctagggcagc ggcgatcccc
    47521 ggtgaagccc ccgtggacac gcaggggtta gccgcacggg cttaccaccc cggggcgacg
    47581 tccgttcgac cgttggtcgt caacttcgtc agggagcctc cagacgcggc aactcgatat
    47641 atctcccagt cgttgtcggt gtcgatcccc tggaacacga tccgaaggcc ggcccccgac
    47701 caggctggga gcgcgtcgtc cttcgccttc gtcgtcagcc ggatgacgga ggacccgtcg
    47761 acgttcatcg cgtagatgtc gaggttgttc acggcctcac ggttcgacgt gaaggcgatc
    47821 ctcgtccccc ccggggacca ggccggggac aggtcacggc ggttgttggc cgtcagcggt
    47881 tgctgccccg agccgttcgc gttcatgacg aagatctcct cgtcgccgtc gcgcgtggac
    47941 atgaacgcga tcttcgtgcc gtccggcgac caggtcgggg tggtgtccgt ggataccacc
    48001 gtcgtcaggc gctcggcgac gcccgtcccg tcggcgttca tcgtccagac gtcgtagttg
    48061 ttcttcgtga tccggttgga gacgaacgcg atcttcgtgc cgtccgggga ccagaccgcg
    48121 gcctgatcga ccgccgtgtt ggtcgtgagc ttcaccacgt cgctgccgcc gacgtccatg
    48181 acgaagatct cgtagtcgcc gtcgcggttc gactcgaacg tgatcttcgt gccgtctggt
    48241 gacagcgagg gatcggtgtc gaccgtcgag ttcgtcgtga gccggacgac gctggcgagg
    48301 gacccgtcgg gggccatcga gtagatctct tgatggttgg cattgttcga gccgtgacgc
    48361 gtggacgaga acacgatggg cctttcgacc aggaagtccg ccaccgtctc ggcatctgcc
    48421 ccgaagctga cccaatcctg gctcgtctgg tccgaaccgt cgagcgtcct cgccctccag
    48481 tgatagccgg tcgcccgaag gaggccggtg accgtcgcgc tgccgatcga gccgcttccg
    48541 acagccgtcg atgtgacgag gccgctcccg tcgaaagccg taccgaccgg cttgacctcc
    48601 acctgcaatc ggaccgatcc gccgtcgggg tctgaaacgg tcgccttcag gaccacgctc
    48661 gtcccgtccg tcgtcgctcc taccgggatc gatgtggtcc cgttggcttc gaattgcgcg
    48721 agaccggatg ggtcgtcggg atccggatcc ggcgggagcc agaccacatc gacgggagtg
    48781 tcccgctcga tcgtcgttcc gtcgccgagt tccccgaaga agttgagacc ccagcacttc
    48841 acgttgccgc ttcccacgat ggcgcacgaa tggttgtccg aggcggagat ggcctgaacg
    48901 ccactcgaga gccctgaaac gtccaccggc tccatccgcg gggtcgtcga gccatcgccg
    48961 agctgcccgg cttcgttgcg gccccagcac ttggcggcac ctgcatccac gatggcacag
    49021 gtgtactggc ctcccgactc gatatcgacg acctccgtgg tgagtccggc gacgtcatcg
    49081 gcggttcgac gagatgtcgt cgtcccgtct ccgagctgcc cttcgacgtt gcgaccccag
    49141 cacgaggcgc cagcgctcac gatcgcgcag gtgtgttgac ctccgggcga gatgtcggag
    49201 atcggctgtc cgacgaccac gacgtccgag gctgtgttcc ggtccgacgc cgtgccatct
    49261 cccacctggc cgaagtcctc gttggcgccc caacacttcg ctccgccgcc agcgatcctc
    49321 gcgcatgcat ggttgtcgcc ggccgcgagc ccgaccgccc ctgacgacag tccgtccacc
    49381 ggagtgggtg agtcgatcga cgtgaaccac gggggcgacg ggtctccgta cccgagctgc
    49441 cccctattcg cagcccccca gcactcgacg ccgccggccg cgagcaccgc acaggtgtgg
    49501 tacgagcccg cggcgatcgc cgtgaccccg ctgaggagat cgggtaccgg cgtcggcacg
    49561 agccgatcct ggtgcgtccc gtcgccgagc tgcccggcct ggttgaagcc ccagcacacg
    49621 accgctcccg aggccgtgag cgcgcatgcg tgattcgctc ccgcggcgat ggccgtgacg
    49681 cctgcatcga ggccttggac ggacttcggg ttcagcctgt cgagctgggt cccgtctcca
    49741 agctccccgt gatcgttgcc tccccagcac caaaccccgc cctcgctgct cagtgcgcac
    49801 gtgtgctgac cgcccacagc gatctggacg atcaacggtg gggccgccag cgcgtccagc
    49861 gatggatcac caggtccgat gtcgtagttg tcgagggtgc tgacgggaac gtcctgggga
    49921 ctccacgccc acgcgaggaa gccactcacg ccagcagaga actgtgtgtc gatcttcacc
    49981 cggaaggcat ccgctctgtg ctggagggtc cccccgacgt cgttcgggat gatcccggcc
    50041 tctccgacga acaatggctt gcccaagtca tcgcactggt cgatccggag ctgaagcccg
    50101 ttgaactcat ctcccggcat ccccgcgttc ggagccccgt agtcgtggta ttcacagagg
    50161 tcgatcgccg ggatcgcgtg gatgtccttg taccgcggtc cgtcggcccc gcattgacca
    50221 ctcccgatcg ttcccaggct gacgagatgg ttggcgtcga tcgacttgac cagccccgag
    50281 acgtcagtgg cccagtcgtg cagcgtctgg tagggctgga gatctcctgg agggcaggga
    50341 gtcacctcgg cttcgttgat caactgccag aaggcgatcc ggggatcgtc ctggtaccgg
    50401 gcgacgacct cggcaaccca atcacgatag gagacgagca tccctggctg cacctgttgg
    50461 tagccggtca cgtaccaatc cgccgtcttg tagccgttgc cggcgacatc ggagccgcac
    50521 tcaccccatt ggtccgtgag cgtcgcgatc accttcttgc cgtgggcggc agcgacctgc
    50581 agcgtgtggt cgaaggcgga ccagtcccga tcacgtgtga tcttgtcgat ggcgagcggc
    50641 tggaagaacc acgatcggaa ggttgaggca cccgacccca tcgatgtgag cgcgtcgtcg
    50701 aggcgcgtgc cctgagccat ctgcgccccg caccatccgt cgctgttcgc gttgaagatg
    50761 ttgagtcccg agaagcggta tgggttgccg tcgagcagca gttcggttcc ctggcgcgtg
    50821 atgaaaccgg taggcaccgc cgctgccggc gacgccacga ggaggggacc gaccaggacg
    50881 agcgcgacga gtgccgaacc catgagatgg agtcgctcga agcgcctgcg gacgaacccc
    50941 gccaaacctt cctccccctc gcccgtcgcg ctacagcggg gagcgtattg cctcctcagg
    51001 gaacggtcaa ccgtcatcgg gcggtggaac acgagcggaa cgcaggaccc cggctatcgg
    51061 ccggcgggct ggaacaactc cacgacgttg tcggacggat cttcgaggag gatctgtttg
    51121 ccgcccgggc cttcgatgac gtcgttccgg aacctggcgc ccgcgtcgcg cagacgggcg
    51181 acctcggtat cgatgtcgtc gacgatgaag tggatccggt tccatccgcc cggtccgggc
    51241 ttcgtgccgt cgggcatcgg acgtcccgcc gagctggtcg gtcccgcgag cagcaggcgg
    51301 agatcgcgcc gcacatccgc gaacgcgggc gacgcgttgg tcaggagctc gaagccgagg
    51361 agctcggtgt agaaggcgat cgcctcatcc acgtcatcga ccatgtagcg aacgttgacg
    51421 gtctcttcat ccggcatctg aggacctcct cttcggccct cggtcggaca cgagcacgga
    51481 accccttgcg ggtcaccaac tcctcggcag tccctcacgc tccgtgccag gagcgagggc
    51541 tgcatccatg cagggaccct accaagcagg tatgagacga tcgcacgtcc tcgatcggaa
    51601 cgaccctgat gccgtccgag gaccgccgct aggcttggcg tcgtgccgaa gaccgacgag
    51661 taccgggcgg cgctcgaggg cctcgcccgg tgggagccgt acctgctcga tcattcgggc
    51721 ctcccagggc cacgaggcaa catcgagctc ggccaagcgg cggccgatgt cggcacggtc
    51781 gagcgattcg acgcgttcct ccgatggacg ccggaacgcg ctccgatcgg ctcgaaggag
    51841 gagttcctgg cgttctgcgg cacggtcggg cttggaaagc tcgccgccgc aggctcccgg
    51901 gaggtactcc ccaggctccg cgagttggcg tcagatcctc gctggcgtgt ccgggaagcc
    51961 gtcgcgatgg cgctccagcg ggtcggggcc gtcgatatgc cggcgctcct tcgcgagatg
    52021 ggctcgtggg caaagggcaa cgacctcgaa cgcagggccg ctgccgcggc gctctgcgag
    52081 ccggcgctcc tcgggaagga gaaagacgcc gtcgccgtgc tcacgatcct cgaccggatc
    52141 accgcggacc tcacccgttc gaaggatcgt tcgggcgatg acttccgggc actgcggaag
    52201 ggactcgcgt actgctggag cgtcgcggtg tccgcggcgc ccgaccgcgg tcgcgagctg
    52261 atggaacgat gggtacggag cgacgacccc gacgtccggt gggtgatgaa acagaacctc
    52321 gcgaagaagc gcatcggcgt ggtcggcgcc ggatgggtcg cagggatgac cgcagagctc
    52381 gagtgatgcc cggcggcgtg cgccgaacga tcgccggtga aagacccgcg tttcagcggc
    52441 ggccgcggcg acgcggactc gcgggatcac cggccgggga ggacgacccc ccgacgggct
    52501 ggtcctcagg cagatctggg atccggatcg atgcggaacc cgtctcgctc cccaactcga
    52561 cgctgatcac gtgatcgaac agccccgggt cgctcggctc gagccgcact gcgctgatcc
    52621 tgtcggatcg gtcgaacgag aacgaggcat ggagcgtcat ctcgacgcgg tgcagctgcc
    52681 ccctgatggc gatgtcacgg tgcgcggtca tccgatcccc atcgtgcgcg aacctgatca
    52741 gatgagacgt gcaggtgacg taccgcccga gcccgatcgc gagaccggcg acggcgtctc
    52801 gcccatggtg tgtcccggcg agcggggagg atccggtcag cgtcaggacg acgtcctcac
    52861 gcgtcaggct gcgcagcatc ccgatgtcgc cctgcacgaa ggcgatcgcc acatgtccga
    52921 agatcccctc atgggccgcc tcatccccca tatcgaaaac gtcggcacca gcggctccgg
    52981 aacttgagcc gctcagggac gatccgccgc cgacagaacg gatcggcaac actgcctgat
    53041 caccgggagg gtcagatcgg cactgagctg cttgtccgtc gacggacagg ctgagcgcga
    53101 cgaaccggtc cgactaccag tagcgagccg tggccatcgc cgtggacgcg aggaagagaa
    53161 gcaggagatc cagcttcccg tgccgttcga gcgcggcccc cagtcgatcc agctcggcct
    53221 gctgctgcgg cgtcggcggt ccacccgagg ccgccacctc gccaccgagg tccaccatcc
    53281 gctcgacgtt gcgaccgacc ccgaggtacc ccacgaaggc ggtgatcgtg gccaggagac
    53341 caccgatcgt cagcccgagc ccgaagctcg aggtgaccca gtcgcccagg gagcccaccg
    53401 catccatgtt gcggaaccac ag
//
LOCUS       DASUYD010000045        11637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_464971, whole genome shotgun sequence.
ACCESSION   DASUYD010000045 DASUYD010000000
VERSION     DASUYD010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464971"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VF195_02650"
     CDS             <1..862
                     /locus_tag="VF195_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX6579755.1"
                     /translation="GVAPGQLRRDLDLLFMSGLPPYGPGDLIDVDVDEDGHIWISMAD
                     HFSRPLQLTRIEALAVYLRGTELLALPGMPDAPALAKALEKLQRSLGADALREAEGRI
                     EIARRGRAPEHLDLLRAAARAHERVSIDYFAASTGEWSHREIDPEEVFSGMGHWYVAA
                     WDVDADAERLFRADRVRSAAPTGQTFTPRGLRGAGRDLYSPTGEDVSVRLRLRPAARW
                     IAEYYATTELEERDDGGVEATLPARQLGWVARLLVRVGDDAEVVEPAELRDEVKRLAR
                     ETLSGYGHKT"
     gene            936..1313
                     /locus_tag="VF195_02655"
     CDS             936..1313
                     /locus_tag="VF195_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579756.1"
                     /translation="MTTIRTTCPRCGEVDMGPEAISLSVRSSGREGSYRFTCPRCDDA
                     VEKRADRKIVALLVSAGVDLEPRGSAELSESLFDQEPTEAVEPLEHAMDGPAFSIDDV
                     IEFHFLLEDDHYLKEFLAGSEES"
     gene            1362..1610
                     /locus_tag="VF195_02660"
     CDS             1362..1610
                     /locus_tag="VF195_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579757.1"
                     /translation="MEAWLVVWLVVGLVTTVALLVMLGFLVRHVILLGRTAGRMQEEL
                     QPIADEVAAASARAADSASRLSERASPRGTSARRRGPR"
     gene            1639..2133
                     /locus_tag="VF195_02665"
     CDS             1639..2133
                     /locus_tag="VF195_02665"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX6579758.1"
                     /translation="MLNVGPLELLVVLAVALIVVGPERLPELARSVGRVLRQFREVQD
                     EVRDMVSSGVDDDMRQAADEFRKATGGIKRATDVKGAVRRAERTIRDETAALRTPRTP
                     GSKPAGRSSSPAAAPAEPSEAAAPPADQLVEDSGTTPTSGDASGDTHTSGGTPPDPES
                     TPDR"
     gene            2130..2912
                     /gene="tatC"
                     /locus_tag="VF195_02670"
     CDS             2130..2912
                     /gene="tatC"
                     /locus_tag="VF195_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX6579759.1"
                     /translation="MRTLRRWRRKTPEQREGAMSLIDHLKELRHRLLICLWALLGGSV
                     AGFLLYEPAMRVIQSPYCDFLRDNPDKTPFPQQGCSLIFLGAIDGFLITVKVIVFLAL
                     VVALPVILYQLWAFIVPGLTDRERRWAIPFVALSLVLFLLGGLAAYLTLPKALQFLLG
                     LGGDFVSPLLTVDKYIGFVIFTILAFGLGFEFPILLISLTAAGVLTNETMRRYRRQVV
                     LGLAIFAAVITPSPDPISMLALLIPLYLFYEGAIIVSRLMKR"
     gene            2923..3648
                     /locus_tag="VF195_02675"
     CDS             2923..3648
                     /locus_tag="VF195_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579760.1"
                     /translation="MAIKGKGRTKTRQPVRAPRRGPVPVPMPFLRRPGVHALAAFLAG
                     LLVFWGGVWLTNGLRAQDRTTRAEEQDLLRRRAGASWKSFIETEVGTIGTVQDGSPPV
                     VLPEVRTTLTELGERTPADAVGALQDAAAGAKEVSGGIESYDLSSSLTGKGFDKGQVL
                     RFLSARDELLSSLTSIREAALLGVAAARLDVADRAGVLGRAQSLLAEADAAMTRFQTH
                     HVEALSAAGINPQQPGLPGLPGT"
     gene            3655..4569
                     /locus_tag="VF195_02680"
     CDS             3655..4569
                     /locus_tag="VF195_02680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6579761.1"
                     /translation="MAGAPDLVVVANPAAGHGKAGKLIGSVTTALRRLGVAHEIRVSE
                     SGPDVERLARAASEDGARIVVALGGDGTASLVGNGILGTGTALAVLPAGTGDDFAKAI
                     GAGRLRTAIELLADPKTADLDVIEVTAGVVTRSFLNIAGVGFDSEVNETANGMTVKLG
                     ATGTYVAALIKTLSRFSPAAFVVTVDDERMEGDAMLVEVGSGRWTGGGMRVLPDAVMN
                     DGLLDVCIVQALSKAAFLRAFPRVFIGSHTTHPKVTMRTGTKVEVEANRRVLVYADGE
                     PIGPLPAIFEIRPGALAVVVGPDAKAVR"
     gene            4574..5305
                     /locus_tag="VF195_02685"
     CDS             4574..5305
                     /locus_tag="VF195_02685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6579762.1"
                     /translation="MTTGLLLIHAFPLDARLWEPQLGSLGARLPLVAPHLPGFGATEG
                     PDVLTMGAAADRCLGALDEAGLDTAVVCGLSMGGYVAFELWRSARPRVAALILANTKA
                     EADQPEAAVKRRELAERLRTEGHGFLVDEPPPLLREDAPAELRERVRGWIAEQTPNAI
                     AAASLGMAERPDSVQDLAAIDVPTLVITGSEDRLIPPDVTAGIADGIAGAELLRIEGA
                     GHLSNLEAPEAFDTALMGVLTGLGL"
     gene            5366..8023
                     /locus_tag="VF195_02690"
     CDS             5366..8023
                     /locus_tag="VF195_02690"
                     /inference="COORDINATES: protein motif:HMM:NF019758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6579763.1"
                     /translation="MTTPEDFARQFPFALDAFQHEAIDAVHRGMSTLVAAPTGSGKTV
                     VAEFAIERALDRGAKAFYTTPLKALSNQKFGDLVAKHGAGTVGLLTGDNAINPEAAVV
                     VMTTEVLRNMLYERSSTLTGLHAVVMDEVHYLQDPYRGAVWEEVLIHLPPSVSVIALS
                     ATISNAEEFGEWITTLRGDTRVVIEERRPVPLEHHYLVGPRLHPMHIEQDGVLMPNPY
                     VVSLDQHELKTKTYYRRGSGLPQHQRISRPREGYRRVYVPRREEVVDVLAQEGMLPAI
                     YFVFSRSGCEKSVGWLRDAGVRLTTREEAATIRDRAETRAAWVDEDDLTSLGFAEFLE
                     SLTTGVAAHHAGMLPMFKETVEELFQEGLVKVVFATETLSLGINMPAKSVVIEDLWKF
                     QGERHELVTPGEYTQLTGRAGRRGIDALGHAVVVYQRQVPFERVAGLAATRTYDLASS
                     FRPSYNMAVNLVRNYTPEQAHHLLNSSFAQFLADRGVVVLERSRQQDLATLEGYRKNM
                     TCHLGDFEEYWGYLEKARRLRDEDRRARDRGRRDDVRSGVAKLRPGDVVWVSRARRRG
                     LAVVVSSRDGKPTVLAQDRDFFRLTPRDFEEPPQVLTKIPLPRSGSVRSPRFRRDVAA
                     RLVALDVKTGGSKRRAQTNPKVEREAARLEELAAAHQCRRCPERGKHERWASRASELQ
                     RRLGGVERRIKVRTETLARQFDRVLSVLRALGYVDDWELTVKGQTLTRIYGEGDLLVG
                     EAIASGLLDDLGPSEVVALLSAVIYEGRERIPLSGEMPTAETKERYERLQRLWRKVRR
                     TEDEHQVQLCRELEAGFARPIHQWAEGEALDDILAETDMAPGDFIRNCKQLVDLLRQI
                     EDVASPGTAAQVRAGRAAILRGVVAYTGV"
     gene            8051..8968
                     /locus_tag="VF195_02695"
     CDS             8051..8968
                     /locus_tag="VF195_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6579764.1"
                     /translation="MTSPFGTIAVIVDPRAGIISQLPLLERALDERGLEHRTLVANGS
                     DPSALAASALDDGYRFLVAMGNDATIQDVVNGMFREGRTIREEPVLGVIGAGSGCDLV
                     RSFGIPGDTESAAAHLLGDNTYGLDLMKITATGVTGGRHTRYGHNLAQVGMGAQQVAC
                     AAKAPAWLGGSRRFYGFWCGYLKTKASRVTVEHDRRTWEGRAWNVVVANAQFTGGGLR
                     LSPRSFPGDGVLDALVFTGPRSGAYTQLPRLFRHGDHVPDPHIQELKAKIRFEIRSER
                     PLPVIADGVALGTTPVTFQIMPRSILFKL"
     gene            8965..10062
                     /locus_tag="VF195_02700"
     CDS             8965..10062
                     /locus_tag="VF195_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006142848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6579765.1"
                     /translation="MSAPHLDRVRRAILEAGKRDLDALVVSPSPDLAYLTGYDPMPLE
                     RPTLLVLRPERNPVLVVPELERPLAAASPMGEHLELVPWLDGADPYEVVASLLIGAAR
                     VAVADRLWASHLIRLQRALPGASFSPASAVLGRLRSVKDASELEALRRAAGAADETFR
                     EILGMSFEGRSEEAVAHDLAELLVRHGHAKAEFTIVASGPHAASPHHEPGGRTILFRD
                     AVVMDFGGELGGYLSDTTRTVVVGEPPEGFEQAYAAVQEAQTAAVDAVRPGVRAEEID
                     RAARAVIEAAGYAERFIHRTGHGIGLELHEPPYMVEGDRTILGAGMTFSVEPGLYLEG
                     RFGIRIEDIVAVTEDGVERLNRSTRDLQIVA"
     gene            10617..11024
                     /locus_tag="VF195_02705"
     CDS             10617..11024
                     /locus_tag="VF195_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579766.1"
                     /translation="MSWGAVNELGTLTGYHRIIAKTEHPVLIQLLQAVIKDERRHFAF
                     YRTQALARSRQARTMTRWALEHLWAPVGTGVRPQSETDFVIHSLFGDADGIVAVKEME
                     ATLGELPGLERSRFLTDALEGAATRLGLSLGIG"
     gene            complement(11029..>11637)
                     /locus_tag="VF195_02710"
     CDS             complement(11029..>11637)
                     /locus_tag="VF195_02710"
                     /inference="COORDINATES: protein motif:HMM:NF013565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6579767.1"
                     /translation="VVSIWAHGGFLQMGGQKMAKSAKNIKRVTDLAEQGIDPLAFRWF
                     TFQTRYRSEMDFTWEALETADHRVKQLRRHVAEWGAPVDDLGPAAKEFDSRFREAVAN
                     DLDMPAAVRVVNDLDHSADVSTGEKKGLLATWDRVLGLDLEREARAAWEPTEAVRQLV
                     AQRDSARASKDYARSDELRDELQAMGLEVMDTPEGTKVRPRD"
BASE COUNT         1737 a         4042 c         4070 g         1788 t
ORIGIN      
        1 cggcgtggcg cccggccagc tgcggcgcga tctcgacctc ctgttcatgt cgggcctccc
       61 tccgtacggc ccgggtgacc tgatcgacgt cgacgtggac gaagacggcc acatctggat
      121 ctcgatggcc gaccacttct ctcgacccct tcagctgacg aggatcgagg cgctcgccgt
      181 ctacctccgc gggacggagc tcctcgccct gccggggatg cccgacgccc ccgccctcgc
      241 gaaggcgctg gagaagctgc agcgctccct cggcgcggac gcgctgcggg aggccgaagg
      301 acgcatcgag atcgcgcgac ggggacgagc gccggagcac ctggatctcc tgcgagcggc
      361 cgcgcgcgca cacgaacgag tgtcgatcga ttacttcgcg gcctcgacgg gggagtggag
      421 ccatcgcgag atcgatcccg aggaggtctt ctctgggatg gggcattggt acgtggcggc
      481 ctgggacgtg gacgcggacg ccgagcgtct cttccgcgcg gatcgggtcc ggagcgcagc
      541 gccgaccggc cagacgttca cgccgcgtgg cctgcgggga gcggggcgag acctctattc
      601 gcccaccggg gaggacgtgt ccgtgcgcct gcgactgcga ccggcggcgc gctggatcgc
      661 cgagtactac gccacgaccg agctcgagga acgagacgac ggaggagtcg aggcgacgct
      721 tccggcgcgt cagctcgggt gggtggcgcg gctgctggtt cgcgtcgggg acgatgccga
      781 ggtcgtagag cctgccgagc tccgagacga ggtgaagcgg ctcgcccgcg agaccctgtc
      841 gggctacggc cacaagacct agtcagacgg gtctagcccc gacaacgctc gcgagcctca
      901 aggcgcgtcg tcggagggcc gatagctgtc gtgagatgac caccatccgg acgacatgcc
      961 cccgctgcgg agaggtcgac atgggccccg aagcgatctc gctctcggtc cgttccagcg
     1021 gccgggaggg gagttaccgc ttcacgtgcc ctcgctgtga cgacgccgtc gagaagcggg
     1081 ccgatcgcaa gatcgttgcc ctgctcgtgt ccgccggcgt ggatctcgaa ccgagaggga
     1141 gcgcggagct gagcgagagc ctcttcgatc aggagcccac ggaggccgtc gagccgctcg
     1201 agcacgcgat ggacggtccc gcattctcga tcgacgatgt gatcgagttc cacttcctcc
     1261 tcgaggacga ccactacctc aaggagttcc tggccggctc ggaagagtcg tagttcccga
     1321 ccacacgcct cccacacgta cgcccgggta gcatccctga cgtggaagcc tggctcgtcg
     1381 tctggctcgt tgtcggtctc gtgaccaccg tcgccctgct cgtgatgctg gggttcctcg
     1441 ttcgccacgt gatcctgctc gggcgaacgg ccgggcggat gcaggaggag ctccaaccga
     1501 tcgccgatga ggtggccgcc gcctccgccc gggccgccga ctcggcatcc cgcctctcgg
     1561 agcgcgcgtc ccctcgaggg acatccgccc gccgcagggg tccccgatag actcgctgcc
     1621 gggcacgtcc cgagaccgat gctcaacgta ggacccctcg agctcctcgt cgtcctcgcc
     1681 gtcgcgctga tcgtggtggg gccggaacgc ctgcccgagc tcgcccgttc cgtcggccgc
     1741 gtgctccgcc agttccggga ggtccaagac gaggtccgcg acatggtgtc gtcgggcgtc
     1801 gacgacgaca tgcggcaggc ggcggacgag ttccgcaagg ccaccggggg catcaagcgg
     1861 gcgaccgacg tgaagggcgc cgtccgtcgg gccgaacgga cgatccggga cgagaccgcc
     1921 gcgctccgga cgccccgaac cccaggctcg aaaccggccg gtcgttcctc gtccccggct
     1981 gcagctccag ccgagccatc cgaagcggcc gcaccgcccg ccgaccagct ggtggaggac
     2041 agcgggacca ctcccacgtc cggcgacgcc tccggtgaca cccacacctc cgggggcaca
     2101 ccaccagacc ccgagtcgac accggaccga tgaggacgct ccggaggtgg cgccggaaga
     2161 cccccgagca gcgcgagggc gcgatgtcgc tcatcgatca tctcaaggag cttcgccacc
     2221 ggctcctgat ctgcctgtgg gccctgctgg gcggctcggt ggccggcttc ctcctctacg
     2281 agcccgcgat gcgcgtgatc cagagcccgt actgcgactt cctccgcgac aacccggaca
     2341 agaccccgtt cccgcaacag gggtgctcgc tgatcttcct cggcgcgatc gacgggttcc
     2401 tcatcaccgt gaaggtgatc gtcttcctgg ccctcgtcgt ggcgctcccg gtgatcctct
     2461 accagctttg ggcgttcatc gtgccgggcc tcacggatcg cgagcgtcga tgggcgatcc
     2521 cgttcgtggc cctctcgttg gtcctgttcc tgctcggcgg gctggccgcc tatctgacct
     2581 tgccgaaggc gctgcagttc ctgctcgggc tgggcggcga cttcgtgagc ccgttgctca
     2641 cggtcgacaa gtacatcggg ttcgtgatct tcacgatcct cgcgttcggg ctcggcttcg
     2701 agttcccgat cctgctgatc tccctcacgg ccgcgggcgt gctcacgaac gagacgatgc
     2761 gacgctaccg gcgacaggtg gtcctggggc tcgccatctt cgcggccgtg atcacgccga
     2821 gccccgaccc gatctcgatg ctcgcgttgt tgatccccct ctacctgttc tacgagggag
     2881 ctatcatcgt cagccgcctg atgaagcgat aggggcgaga cgatggcgat caagggcaag
     2941 ggacggacga agacgaggca acccgtgcgc gcgcctcggc gcggccccgt cccggtcccg
     3001 atgccgttcc tgcggcggcc aggggtccat gccctggccg cgttcctcgc cggcctgctg
     3061 gtcttctggg gcggcgtctg gctcacgaac gggctccgcg cccaggaccg cacgacacgc
     3121 gcggaggagc aagacctcct ccgtcggcgc gccggcgcct cctggaagtc gtttatcgag
     3181 accgaggtgg gaacgatcgg aacggtgcag gacggctcgc ctcccgtcgt cctcccggag
     3241 gtgcggacca ccctcaccga gctgggggag cgcacacccg cggatgccgt cggagcgctg
     3301 caggacgcgg cggcgggcgc gaaggaggtc tcggggggga tcgagtccta cgacctctcg
     3361 agctcgctca ctggcaaggg cttcgacaag ggccaagtgc tccgattcct ctcggcgagg
     3421 gacgagctgc tctcctcgct cacgtcgatc cgcgaggccg cactcctcgg cgtggccgcc
     3481 gcgcgtctcg acgtcgccga ccgggccggc gtgctcggcc gggcacagtc gttgctcgcc
     3541 gaggcggacg ccgcgatgac ccgcttccag acccaccacg tcgaggcgct cagcgccgcc
     3601 gggatcaacc cgcaacagcc gggtcttccg ggtctgccgg gcacgtagga gcgcatggcc
     3661 ggcgcaccgg acctggtggt ggtcgcgaac ccagcggccg gccacgggaa agcggggaag
     3721 ctgatcggct cggtcaccac cgcgctgcgc cggctcggcg tcgcacacga gatccgcgtg
     3781 tccgagtcgg gcccggacgt ggagcgtctc gctcgggccg cctcggagga cggggcgagg
     3841 atcgtggtgg cgctcggagg cgacgggacg gcctcgctcg tcgggaacgg gatcctcggg
     3901 acgggaacgg ctttggcggt cctgccggcc ggcaccggcg acgacttcgc gaaggcgatc
     3961 ggcgcaggga ggctccggac cgcgatcgaa ctgctggccg acccgaagac cgccgacctc
     4021 gacgtgatcg aggtgaccgc gggcgtcgtc acgaggagct tcctcaacat cgccggcgtc
     4081 gggttcgact ccgaggtgaa cgagaccgcg aacgggatga cggtcaagct gggtgcgacg
     4141 ggcacgtacg tcgccgcact gatcaagacg ctctccaggt tctcgccggc cgcgttcgtc
     4201 gtgacggtcg acgacgagcg gatggagggc gacgcgatgc tcgtcgaggt cgggagcggc
     4261 cgctggaccg gcgggggcat gcgggtcctt ccggacgccg tcatgaacga cggactcctg
     4321 gacgtctgca tcgtgcaggc gctctccaag gccgcgttcc tgcgggcgtt cccccgcgtc
     4381 ttcatcgggt cacacaccac gcaccccaag gtcacgatgc gcaccgggac gaaggtggag
     4441 gtggaagcga accggcgtgt gctcgtctac gcggacgggg agccgatcgg accgttgccc
     4501 gcgatcttcg agatccggcc cggcgcgctc gccgtggtgg tcggtccgga cgccaaagcc
     4561 gtacggtgac ctcatgacga cgggcctcct gctgatccat gccttcccgc tcgacgcccg
     4621 gttgtgggag ccgcagctcg gctcgttggg cgctcggctc ccgctcgtcg ccccgcacct
     4681 cccgggcttc ggtgctaccg aaggcccgga cgtcctcacg atgggtgccg cggcagaccg
     4741 gtgcctcggc gcgctcgacg aggccggcct cgacaccgcg gtcgtgtgcg gcctctcgat
     4801 gggtgggtac gtggcgttcg agctgtggag gtctgcgcgg cctcgcgtcg ccgccctgat
     4861 cctggccaac acgaaagccg aggccgacca gcccgaggcc gcggtcaagc gtcgggagct
     4921 ggccgagcgc ctgcggaccg agggccacgg gttcctggtc gacgagcccc cgcccttgct
     4981 ccgagaggat gctccggcgg agcttcgcga acgcgtgcgg gggtggatcg cggagcagac
     5041 cccgaacgcg atcgcggcag cctcgctcgg gatggccgag cgtccggact cggtgcagga
     5101 cctcgcggcg atcgatgtgc ctacgctggt gatcaccggt tcggaggatc gcctgatccc
     5161 ccccgacgtc acggctggca tcgccgacgg catcgccggg gccgaactgc tccggatcga
     5221 gggcgccggc cacctgtcca acctcgaggc gccggaggcc ttcgacaccg cgctcatggg
     5281 cgtgctgacg gggctcggtc tctagcccca cgccccgcgg gcgcccgcca gggcaacgga
     5341 ggaccgcccg tagactggcc ccaccgtgac cacgcctgag gacttcgcgc ggcagttccc
     5401 cttcgcgctc gacgcgttcc aacacgaggc gatcgatgcc gtccatcgcg ggatgtcgac
     5461 cctcgtggcc gcgccgaccg gttcggggaa gaccgtcgtc gcggagttcg ccatcgagcg
     5521 cgcgctcgac cgcggcgcca aggctttcta cacgaccccg ctgaaggccc tgtcgaacca
     5581 gaagttcggc gacctcgtcg ccaaacatgg cgcggggacg gtcggtctcc tcacgggcga
     5641 caacgcgatc aacccggagg ctgccgtggt cgtcatgacc accgaagtcc tccggaacat
     5701 gttgtacgag cggtcgtcca cgctcacggg cttgcacgcc gtcgtgatgg acgaggtgca
     5761 ctatctgcag gacccctacc gtggcgccgt ttgggaggag gtcctgatcc acctcccgcc
     5821 gtccgtgagc gtgatcgcgc tctccgcgac gatctcgaac gcggaggagt tcggcgagtg
     5881 gatcacgacc ctccgtggcg acacccgcgt cgtgatcgaa gagcgccgcc cggtaccgct
     5941 cgagcatcac tacctcgtgg gaccccggct gcatccgatg cacatcgagc aggacggggt
     6001 cctgatgccg aacccctacg tcgtctccct ggaccagcac gagctgaaga cgaagaccta
     6061 ctaccggcgc gggtcggggc tgccgcagca ccagcgcatc tcccgacccc gggaaggcta
     6121 ccgccgcgtc tacgtccccc gccgcgagga ggtggtcgac gtgctcgcgc aggaagggat
     6181 gctgccggcg atctacttcg tgttcagccg gtccggctgc gagaagagcg tgggctggct
     6241 ccgggacgcg ggggtccgtc tcacgacccg ggaggaggcc gccacgatcc gggatcgggc
     6301 ggagacccgg gccgcgtggg tggacgagga cgacctcacg agcctcggat tcgcggagtt
     6361 cctcgagtcc ctgaccaccg gggtcgccgc ccaccacgcc ggcatgctcc cgatgttcaa
     6421 ggagacggtc gaagagctgt tccaggaagg gctcgtgaag gtggtcttcg cgacggagac
     6481 cctctcgctg gggatcaaca tgcccgccaa gagcgtcgtg atcgaggacc tctggaagtt
     6541 ccagggcgaa cgccacgagc tggtcacgcc cggtgagtac acgcagctca cgggacgcgc
     6601 gggccggcgc gggatcgacg ccctcgggca cgcggtcgtc gtctaccagc gccaggtgcc
     6661 gttcgaacgg gtcgcggggc tcgcggccac gcggacctac gacctcgcgt cgtcgttccg
     6721 accctcgtac aacatggcgg tgaacctcgt ccgcaactac acgcccgagc aggcgcatca
     6781 cctgctgaac tcctcgttcg cgcagttcct cgccgaccgg ggcgtggtgg tgctcgagcg
     6841 atcccggcag caggacctcg cgacactcga gggctatcgg aagaacatga cctgccatct
     6901 cggtgatttc gaggagtact ggggttacct ggagaaggcg cgacggctgc gcgacgagga
     6961 ccggcgcgca cgtgatcggg gacgtcgtga cgacgtccgg tcgggcgtcg cgaagctccg
     7021 tccgggcgat gtcgtgtggg tgtcgcgcgc ccggcgacgc ggcctcgcgg tggtcgtgtc
     7081 ctcgcgagat gggaagccga ccgtcctcgc ccaggaccgc gacttcttcc ggttgacgcc
     7141 gcgggatttc gaggaaccgc cgcaggtctt gacgaagatc ccgttgcccc gatccgggag
     7201 cgtccgcagc ccgcggttcc gccgcgacgt cgcggcccgc ctcgtcgcgc tcgacgtgaa
     7261 gaccggggga agcaagcggc gggcccagac gaaccccaag gtcgaacgcg aggcggcccg
     7321 gctggaggag ctcgctgcag cacatcagtg tcggcgatgc ccggagcgcg ggaaacacga
     7381 gcgctgggcg agccgggctt cagagctcca acgacgactc ggcggcgtcg agcgccggat
     7441 caaggtccgt accgagacgc tcgcgagaca gttcgaccgg gtcctgtcgg tcctgcgggc
     7501 gctgggctac gtggacgact gggaactcac cgtgaagggg cagacgctca cccgcatcta
     7561 cggcgagggt gacctgctgg tgggggaggc gatcgcgtcg ggcctgctgg acgacctcgg
     7621 gccctccgag gtcgtcgccc tgttgtcagc ggtgatctac gagggacgtg aacggatccc
     7681 tctctcgggc gagatgccga cggcggagac gaaggagcgg tacgagcggc tgcagcgatt
     7741 gtggcgcaag gtccggcgca ccgaggacga gcaccaggtg cagctctgcc gagagctcga
     7801 ggcggggttc gccaggccga tccaccagtg ggccgagggc gaagccctcg acgacatcct
     7861 cgcggaaacg gacatggccc caggtgactt catccggaac tgcaagcagc tcgtcgatct
     7921 gctccgccag atcgaggacg tcgcctcgcc cggcacggcg gcgcaggtcc gggccgggcg
     7981 cgccgcgatc ctccgaggcg tcgtcgccta cacgggtgtg tagctacgct tcgcccatgg
     8041 gacctcggtc atgaccagcc ccttcggtac gatcgccgtg atcgtcgacc cccgtgcggg
     8101 catcatctcc cagctcccgt tgctcgagcg cgcgctcgac gagcgtggcc tcgagcaccg
     8161 gaccttggtg gcgaacgggt cggatccctc cgcgctcgcg gcctcggccc tcgacgacgg
     8221 gtaccggttc ctcgtcgcga tgggcaacga cgcgaccatc caggacgtcg tgaacgggat
     8281 gttccgagag ggccgcacga tccgggagga gcccgtgctc ggcgtgatcg gagccgggag
     8341 cgggtgcgat ctcgtgcgga gcttcgggat acccggcgac acggagtcgg cggccgccca
     8401 cctcctgggg gacaacacct acggtctcga cctcatgaag atcacggcca cgggcgtcac
     8461 gggggggcgc cacacgaggt acgggcataa cctcgcgcag gtggggatgg gcgcgcaaca
     8521 ggtcgcgtgt gcggcgaagg ctccggcctg gctgggtggc tcgcggcggt tctacggctt
     8581 ctggtgcggc tacctcaaga cgaaggcgtc tcgtgtcacg gtggagcacg atcggcgcac
     8641 gtgggagggc cgggcttgga acgtcgtcgt ggcgaacgcg cagttcaccg ggggcggcct
     8701 gcgtctgtcg cctcgatcgt tcccggggga cggcgtgctg gacgccctcg tgttcaccgg
     8761 cccccgttcg ggcgcctaca cgcagctccc gcggctcttc cgccacggcg accacgtccc
     8821 cgacccacac atccaggagt tgaaggcgaa gatccgcttc gagatccgat cggagcggcc
     8881 gctgccggtc atcgccgacg gtgtcgcact cggcacgacg cccgtcactt tccagatcat
     8941 gccccggtcg atcctcttca agctatgagc gcgccgcacc tcgaccgggt ccgccgcgcg
     9001 atcctggaag ccgggaagcg cgacctggac gcgctggtgg tgtcgccgtc tcccgatctc
     9061 gcctacctca ccggctacga ccccatgcct ctggaacgcc ccacgctgct cgtgctccgg
     9121 ccggaacgta acccggtgct cgtggtccca gagctcgagc ggccgctcgc ggcggcctcg
     9181 ccgatggggg aacacctcga gctggtgcct tggctggacg gcgccgatcc gtacgaggtc
     9241 gtcgcgagcc tgctgatcgg ggcggctcga gtcgccgtcg ccgatcgcct ctgggcctcc
     9301 cacctgatcc gcctccaaag ggcgttgccg ggcgcctcgt tctccccggc gtccgcggtg
     9361 ctcggtcggc tccggagcgt gaaggacgcc tccgagctcg aggccctgcg ccgtgccgcc
     9421 ggggcggcgg acgagacctt ccgggagatc cttgggatgt ccttcgaggg ccggagcgag
     9481 gaggccgtcg cgcacgacct cgcggagctc ctcgtccgcc atggtcacgc caaggcggag
     9541 ttcacgatcg ttgccagcgg cccgcacgcc gcttcccccc accacgagcc agggggccgg
     9601 acgatcctgt tccgcgacgc ggtggtgatg gacttcgggg gagagctcgg gggctacctc
     9661 agcgacacca cgcggaccgt cgtggtcggg gagcccccgg aggggttcga gcaggcgtac
     9721 gcggccgtcc aggaggccca gaccgcggcc gtggatgccg tcaggccagg ggtccgagcg
     9781 gaggagatcg atcgggccgc cagggccgtg atcgaggccg ccgggtacgc cgagcggttc
     9841 atccaccgca ccggccacgg gatcggcctc gagctgcacg agcccccgta catggtggag
     9901 ggggacagaa ccatcctcgg agcggggatg acgttctccg tggagcccgg cctctacctg
     9961 gagggacgtt tcgggatccg gatcgaggac atcgtggccg tgaccgagga cggggtcgag
    10021 cgcctgaacc ggagcacgag agacctccag atcgtcgctt gaccagccgg cggggtctcg
    10081 ggtgatcctc accccccagg gatgcgagca cgtcccagaa gcgttcgcgg gcgggaggtc
    10141 ggctgcatgg aggaccggac catgaagatg gacacctcgg acttcgatct ggacaggtac
    10201 ctgcgtgcct cgaagcgggt cgacctgtcc accgtggagt gggcgcggat ccgtgagcac
    10261 cccgtgacga ccgccgaagc acgctgcctc acgtacatga tggacatcga gacccatacg
    10321 gtggtgttcc tccgggacct cctcgcgacc cgcgcgtcgt tcgatcccga ggtcaccgcg
    10381 ttcctgtcgt gctgggtcta cggggggtct ggcacggcga ggggttcagc cgcttcctgg
    10441 gcgaggccgg gtaccgggtg gccccgacct tcgaggacgt ttcgggagag gacccgttcc
    10501 caacccgcgt cgaccggaac cgatgggtcc gatagaagtt cgggacgaag ggctatctct
    10561 cccacatcgg cactctgctc ggatcggcgc tgttctgcga cttcgtcgcc gttcacatga
    10621 gctggggcgc cgtcaacgaa ctcggcacgc tgaccgggta tcaccgcatc atcgccaaga
    10681 ccgagcaccc cgtcctgatc cagctcctcc aggccgtgat caaggacgaa cgccgacact
    10741 tcgccttcta tcggacgcag gccctggcgc gatcccgcca ggcgcggacg atgacccggt
    10801 gggcactcga gcatctctgg gctcccgtgg ggaccggtgt ccggccgcag tccgagaccg
    10861 acttcgtgat ccactccctg ttcggcgatg cggacgggat cgtcgccgtc aaggagatgg
    10921 aagcgacgct cggggagctt ccgggcctgg agcgcagccg cttcctcacg gacgcgctgg
    10981 aaggagcggc gacccggctc ggcctatcgc tcgggatcgg ttgagccctc agtctcgcgg
    11041 gcggaccttc gtcccctccg gagtgtccat gacctcgagg cccatcgcct gcagctcgtc
    11101 ccggagctcg tccgagcgcg cgtagtcctt cgacgctcga gcgctgtcgc gttgggcaac
    11161 caactgtcgg accgcctccg tcggctccca cgccgctcgc gcttcccgtt cgagatcgag
    11221 tccgaggacg cggtcccagg tcgcgaggag tcccttcttc tcgccggtcg agacgtcagc
    11281 cgagtggtcg aggtcgttca ccacacggac ggcggccggc atgtcgaggt cgttcgcgac
    11341 ggcctcgcgg aaccgtgaat cgaactcctt cgcggcaggg ccgagatcgt cgaccggcgc
    11401 accccactcc gcgacgtgcc tccggagttg cttcacccga tggtcggccg tctccagcgc
    11461 ctcccatgtg aagtccatct cggatcgata ccgggtctgg aacgtgaacc accggaaggc
    11521 gagggggtcg atgccctgct cggccagatc ggtcacccgc ttgatgttct tcgcggattt
    11581 cgccatcttc tggccgccca tctgcaggaa cccgccgtgt gcccagatcg acacgac
//
LOCUS       DASUYD010000046         3361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1787126, whole genome shotgun sequence.
ACCESSION   DASUYD010000046 DASUYD010000000
VERSION     DASUYD010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1787126"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF195_02715"
     CDS             <1..307
                     /locus_tag="VF195_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004003920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579768.1"
                     /translation="LHELDIFTPNRSGFPLIEIPLADHEDIDVVGRFLFDRGIYVTMA
                     AYPLVPKDEVGFRIQLTAANPDDQVELLCEVLGEMSERFRLQHEHHRLLIRAQEAGG"
     gene            307..1002
                     /locus_tag="VF195_02720"
     CDS             307..1002
                     /locus_tag="VF195_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579769.1"
                     /translation="MAKGPVAEGLGRAEALSDAGEYATALEELDVALGTEPHNAAVFT
                     ARGWALENLGPERLPEARSAYEQALAFDPSAMWAKEGLSNVLRRMGHPREADRLCEEV
                     IEEGRRRYNAEEDLWELVGWCELRLGRHEDAVATLREALRRDGRWIAVRFDLALALLC
                     AGERSRAFLEYSRGVHQAVNGGVDRLRGCLRVALDDLEESLTERPALATTREAIAARD
                     LLRSTLQAAYQPG"
     gene            1007..2431
                     /locus_tag="VF195_02725"
     CDS             1007..2431
                     /locus_tag="VF195_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579770.1"
                     /translation="MGDHRDGRTFIERRIRILTPLRHRDFRLLWTGMTVSLLGDGIFY
                     VSLAWQAYELSNTPSALSTIGVAMTVPHIVFLLAGGVVSDRFDRRRVMIAADLMRALA
                     VGVMGALALSGRLELWHMVVLSGVFGAGTAFFGPAFDAIVPGLVPDDELNQANSLDQL
                     VRPAALRMLGPALGGWIVATGGPGAAFVADACTFAISISCVALMRTRRTSSEEPHASV
                     IADIREGFGYVRSQVWLWGTFLAATLGYLIFWGPSEVLLPFVVRNDLHGTAADLGFIF
                     AVGGIGAMTSAVVMGNRQMPRKNMTFIYAVWTASTLAIAGYGLARFPWQAMAACFAFN
                     ALEGAGTIVWATTKGRLVPSRLLGRVSSFDWFISIALVPVSYAFTGPIADVFGAKQTL
                     VGAGLLGGVVTLAFLFLPGMREIEGSDRLAGTPVDRTSDTGFGALEGPMAPISAGQGA
                     FPGSGTLASSLRPPAASSDIGKQT"
     gene            2428..>3361
                     /locus_tag="VF195_02730"
     CDS             2428..>3361
                     /locus_tag="VF195_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579771.1"
                     /translation="MKSRAWVLYLCGGVAALLGFLLLPHMRQGWVFNLISLSSPVAIV
                     AAVRMWKPTVRAPWYLFALGQTFFVVGDVITYNYEQFFGTELPYPSIGDVAYLLVYPC
                     LVGGLLLLIRERNPGRDRDSVIDSLIVAIGVGTMQWLFLMSPVAHDSASTLLQKIVGM
                     AYPFMDLILVTAVVRLAIGAGKRAPSLYLMGAAAFALFLTDTVYTYISVQGLVYDQSG
                     FLEAGWGMFYLLWGAAALHPSMRHLADRAPDQETRLTRARLILLGAASLIPQAVRAIQ
                     LLREEATDLWIITISTTFLFVLVVARMAGLVRRQE"
BASE COUNT          442 a         1155 c         1153 g          611 t
ORIGIN      
        1 cctgcacgag ctcgacatct tcacacccaa ccgatccggg ttcccgctca tcgagatccc
       61 gctcgcggac cacgaggaca tcgacgtggt gggtcgtttc ctgttcgacc gcgggatcta
      121 cgtgacgatg gcggcgtacc ccctcgtgcc caaggacgag gtgggcttcc gcatccagct
      181 caccgccgcg aaccccgacg atcaggtcga gctcctctgc gaggtgcttg gggagatgag
      241 cgagcgcttc cggctgcagc atgagcacca ccggctgctg atccgcgcgc aagaggcggg
      301 gggctagtgg ccaagggtcc cgtcgccgag gggctcggtc gcgccgaggc gctctcggac
      361 gccggcgagt acgccaccgc gctggaggag ctcgacgtcg ccctcggcac cgagccccac
      421 aacgccgccg tgttcacggc gcggggatgg gcgctcgaga acctcgggcc cgagcggctc
      481 cccgaggcgc gttcagccta cgagcaggcg ctggccttcg acccatcggc catgtgggcg
      541 aaggagggcc tgtccaacgt gctccggcgg atgggccatc cccgggaggc cgaccgcctc
      601 tgcgaggagg tcatcgagga gggccgtcga cgctacaacg cggaagagga tctgtgggag
      661 ctggtcgggt ggtgcgagct ccggctcggg cgccacgagg acgcggtcgc gacgcttcgc
      721 gaggccctcc gtcgcgacgg tcgttggatc gcggtccgct tcgatctcgc gctcgcgctg
      781 ctgtgcgccg gcgaacgctc tcgcgcgttc ctcgagtact cgcgcggcgt ccaccaggcc
      841 gtgaacggcg gcgtggaccg actgcgcggc tgtctgcgcg tggccctcga cgatctcgaa
      901 gagtcgctga ccgaacggcc cgcgctcgcg accacgcgtg aggcgatcgc cgcgcgcgac
      961 ctcctccgct ccaccctgca ggccgcctac cagcccggct gatcggatgg gcgaccaccg
     1021 cgacggtcga acgttcatcg aacgtcgcat acggatcctg acgccccttc gtcatcgcga
     1081 cttccgtctg ttgtggacgg ggatgaccgt gtcactgctc ggtgacggga tcttctacgt
     1141 ctcgctcgcg tggcaggcct acgagctctc gaacacgccg agcgccctgt ccacgatcgg
     1201 ggtcgccatg acggtgcccc atatcgtgtt cctgctcgcg ggtggcgtcg tctcggaccg
     1261 gttcgaccgg cgccgggtca tgatcgcggc cgatctgatg cgcgccctcg cggtcggcgt
     1321 gatgggagcc ctcgccctgt ccggccggct ggagctgtgg cacatggtcg tgctctcggg
     1381 ggtcttcggc gcggggaccg ccttcttcgg tcccgccttc gatgcgatcg tccccggtct
     1441 ggttccggac gacgagctga accaggcgaa ctcgctcgac cagctggtac ggccagccgc
     1501 cctccggatg ctcgggccgg cgctgggtgg ctggatcgtg gcgacgggtg gaccgggcgc
     1561 cgcgttcgtg gcagacgcat gcacgttcgc gatctcgatc tcgtgcgtgg cgctgatgcg
     1621 cacacgacgc acgagctcgg aggagccgca cgcatcggtg atcgctgaca tccgcgaggg
     1681 gttcgggtac gtccgatcgc aggtctggtt gtggggaacg ttcctcgccg cgaccctcgg
     1741 ctacctgatc ttctgggggc cgtcggaggt cctgctcccg ttcgtcgtcc gcaacgacct
     1801 gcacggcacc gcggccgacc tcggattcat cttcgccgtg ggcgggatcg gcgcgatgac
     1861 ctcggcggtc gtgatgggca accgtcagat gccccgcaag aacatgacct tcatctacgc
     1921 ggtctggacc gcttcgaccc ttgccatcgc cggctacggt ctcgcgcgct tcccctggca
     1981 ggcgatggcg gcctgcttcg cgttcaacgc gctcgaaggc gccggcacga tcgtgtgggc
     2041 gacgaccaag gggcggctgg tgccgagccg gttgttgggg cgcgtctcga gcttcgactg
     2101 gttcatctcg atcgccctcg tgccggtctc ctacgcgttc acgggcccga tcgccgacgt
     2161 cttcggcgcg aagcagacgc tcgtcggggc cggcctgctg gggggcgtgg tgacccttgc
     2221 gttcctgttc ctgccgggca tgcgcgagat cgaggggagc gacaggctgg caggcacgcc
     2281 ggtcgatagg accagcgata cgggtttcgg ggccctcgag ggaccgatgg ctcctatctc
     2341 cgctggtcag ggggcctttc ccggctcggg gactttggcg tcgagtctaa ggccgcccgc
     2401 cgcgtcctcc gatatcggaa agcagacatg aagtcccggg cctgggtcct gtacctgtgc
     2461 ggcggcgtcg cggcgctgct cgggttcctg ctcctaccgc acatgcggca gggatgggtc
     2521 ttcaacctga tctccctgtc gtcgcccgtc gcgatcgtcg ccgcggtcag gatgtggaag
     2581 ccgaccgtgc gcgccccctg gtacctgttc gcgttgggcc agacgttctt cgtcgtcggc
     2641 gacgtgatca cgtacaacta cgagcagttc ttcggaaccg agctgccata cccgtcgatc
     2701 ggtgacgtcg cctatctgtt ggtgtacccg tgcctggtgg gcgggctgct ccttctgatc
     2761 cgcgagcgca accccggccg cgaccgcgac agcgtgatcg actcgctgat cgtcgcgatc
     2821 ggggtcggga cgatgcagtg gctgttcctg atgtcgccgg tggcgcacga ctccgcctcg
     2881 accctcctgc agaagatcgt cgggatggcc tacccgttca tggacctcat cctggtcacc
     2941 gcggtcgtcc ggctggcgat cggggccggg aagcgggccc cttcgctgta cctcatgggt
     3001 gcggcagcct tcgccctgtt cctcacggac accgtgtaca cgtacatctc cgtccagggg
     3061 ctcgtctacg accagtcggg cttcctcgag gccggatggg ggatgttcta cctcttgtgg
     3121 ggtgctgcgg cgctccaccc gtcgatgcgg caccttgccg atcgcgctcc ggaccaggag
     3181 acgcgcctga cccgagcgcg tctgatcctc ctgggagccg cgtcgttgat cccacaggcc
     3241 gttcgcgcca tccagttgct ccgcgaggaa gcgaccgacc tgtggatcat cacgatctcg
     3301 acgaccttcc tgttcgtcct cgtcgtcgcc aggatggccg gcctggtccg ccgacaggag
     3361 c
//
LOCUS       DASUYD010000047         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_465679, whole genome shotgun sequence.
ACCESSION   DASUYD010000047 DASUYD010000000
VERSION     DASUYD010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465679"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..574)
                     /locus_tag="VF195_02735"
     CDS             complement(41..574)
                     /locus_tag="VF195_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6579772.1"
                     /translation="MFVTRWWHEMVGEPGGSGGIDDAVLRLHDQGIGAEIMGAGKFGY
                     PGWHEDPAWKGWWGDEPPFHTPVFVLTHHPRPSIEMKGGTTFHFLDAAPAEALETARA
                     AASGQDVRIGGGPTMVRDFVAAGLVDHAHIVVVPILLGRGVRLWDGLEGLEKDYQVEA
                     TSSPSGVTHVTFTRTAL"
     gene            1080..1961
                     /locus_tag="VF195_02740"
     CDS             1080..1961
                     /locus_tag="VF195_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6579773.1"
                     /translation="MEAGMTYTFPADIGQRPVTIVGAGTLGRRIAAVYAAGGADVHVH
                     DMSAEQLDACRQFISDNIDRVRTLLDVHPSQGAGDISLYEELGEAVRAAWMVVESVPE
                     DLELKRQVIGELDRLSDADAIVASNSSSLPTSQMLSDVQHRWRVLNTHYQLPPELNAV
                     ELMSCGETDEGVIPGLISTLPEYGLVPFWVRRESDGFILNRIWGSIKRECLMVVGEGV
                     AMPDDVDAMWAIFAGAGIPPFKLMDQVGLDVVLAIEEHYASVRPALPEGPRELLREYL
                     DRGWLGVKSGHGFYEYG"
     gene            complement(2080..>3452)
                     /locus_tag="VF195_02745"
     CDS             complement(2080..>3452)
                     /locus_tag="VF195_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6579774.1"
                     /translation="DEAIVAAAELSDRYIGDRFLPDKAIDLIDQASARVRLRAKTKPV
                     DTRAMEEEIGRLERERDQAVGAEDYAKANELKTTLQEKQDALSGQKHPRQRAPEVTVE
                     DIAEVVSRATGIPVSQLTQEERDRLLKLEERLHERVVGQDEPVVAVAEAVRRGRAGLG
                     DPKRPVGSFLFLGPTGVGKTELARTLAEVLFGDDDLMIRFDMSEFQERHTVSRLVGAP
                     PGYVGFEEAGQLTEAVRRKPYSVVLLDEIEKAHPDVFNLLLQILDDGRLTDAQGRTVD
                     FKNTVVIMTSNLGAERIQAHARRNEDFEELKEDLMQLLRSHFRPEFLNRIDEIIVFRA
                     LTKEQVIQIAELLLDRVRRRLRAQRIELTFTPDAEAFIADRGYDPEFGARPLQRTIQR
                     LVENALSRMLLDGAIREGDAVTVGVVEGGLDFDVRRPAGSEKKDDKSRKDGKSNQEKE
                     LVADEG"
BASE COUNT          545 a         1113 c         1154 g          640 t
ORIGIN      
        1 gtgggcggct gggaacctcg ggtttgtcct ctgaggcgga tcagagagcg gtacgggtga
       61 acgtcacgtg cgtgactccg ctgggtgagg aggtggcctc gacctggtag tccttctcga
      121 ggccctccag tccgtcccag aggcgcactc ctcggccgag caggataggg accaccacga
      181 tgtgcgcgtg gtcgacgagc ccggcggcaa cgaagtcgcg aaccatggta ggacctccgc
      241 cgatacgtac gtcctggccg ctcgccgcgg cgcgagccgt ctcgagcgcc tctgccggcg
      301 ccgcgtcgag gaagtggaag gtcgtgccgc ccttcatctc gatcgacggg cgggggtggt
      361 gggtgaggac gaataccggc gtgtggaaag gcggttcgtc accccaccag cccttccacg
      421 ccggatcctc gtgccacccg gggtagccga acttcccggc gcccatgatc tcggcgccga
      481 tcccctgatc gtgcagccgc aggaccgcgt cgtcgatgcc gccgctgccg cccggctcgc
      541 cgaccatctc gtgccaccac cgggtcacga acatccactc gtgcatccgg tcgccggcat
      601 ggccgaacgg ggcgtcgcgg ctcaggcctt cgccggtgcc gaacccgtcg agcgagatgg
      661 agaagaactg gatgcgggcg agtgacgtgg ctggtgttcc tttctgtctg gtcgagcttg
      721 gtcgaaatgg tcggacgtcg tgctcgacgc ccgttccata ccctaggcga tgagcatgat
      781 gaaggggcgc caaagagacg ggtctgtacc tatcgcgttg ggttcaggct ggccttgtcc
      841 cctagcgaat gaggttgacc cagagaggca tggcggtgga ggcttgcctc ggagcccacc
      901 gagggtgtgc ggcagtgagg ggagcgccat caagcgcgac ggcgctgatt gcgcttgtca
      961 tggtgacgtg tagttctgga tcggatcctc ggcgctcctg cttgatccat tctgtgtccg
     1021 caacgtcgtg gaagcttggg agtcacggaa tccatagcgc gcaccactat tgatcgtgga
     1081 tggaggcggg catgacgtac acctttccgg cggatatcgg gcagaggcct gtgacaatcg
     1141 ttggtgccgg gacgctcgga cgaaggatcg ctgcggtgta tgcagctgga ggagccgatg
     1201 tccacgtcca tgacatgtcc gcggagcagc tcgatgcgtg tcgccagttc atctccgaca
     1261 acatcgacag agtccggacc cttctggacg tgcatccgtc acagggtgcc ggagacattt
     1321 ctctctacga ggagctgggc gaggctgttc gcgctgcctg gatggtcgtt gagtcggtcc
     1381 ccgaggacct tgagctgaaa cgccaggtga tcggagagct cgaccggctg tccgacgccg
     1441 atgcgatcgt ggcgagcaac tcgtcgtcgc tgcccacgag ccagatgctt tcggatgtcc
     1501 aacaccggtg gcgggtgctg aacacccact atcagctacc gccggaactc aatgcggtgg
     1561 agctgatgtc ttgtggcgag accgatgagg gcgtcatccc tgggctgata agcacgctgc
     1621 ccgaatacgg actcgtccct ttctgggtcc gtcgcgagag cgacggcttc atcctcaatc
     1681 ggatctgggg gtcaatcaag cgtgagtgcc tgatggtggt cggagaaggg gtcgcgatgc
     1741 ccgacgatgt ggacgcgatg tgggcgatct tcgctggcgc cggcatcccg cccttcaagc
     1801 tgatggatca agtcggactg gatgtcgtcc tggcaattga ggagcactac gcatccgtcc
     1861 gaccggcact cccagagggt ccgcgtgaac tcttgcgcga gtacctcgac cgaggttggc
     1921 ttggcgtgaa gagcggacac gggttctacg aatacggata gccgcggcgg agcccgcccg
     1981 ctcagccgca tccgtgtgtg agcgccctgc gaaaaagggc gcaaggaaat cgggtctggt
     2041 aggacccagc gtgtggggag gcgaactcat cgcactcgat cacccttcgt ctgcgaccag
     2101 ttccttctcc tggttcgact tcccgtcctt gcgggacttg tcgtccttct tctccgaccc
     2161 ggcgggcctc cgcacgtcga agtcgagccc tccctcgacc acaccgacgg ttacggcgtc
     2221 accctcccgg atcgccccgt ccagcagcat ccgtgacagc gcgttctcaa cgagccgctg
     2281 gatcgtccgc tggagcggac gggccccgaa ctcggggtcg tacccgcgat cggcgatgaa
     2341 cgcctcggcg tccggcgtga acgtcagctc gatccgctgg gcgcgcaggc gccggcgaac
     2401 ccggtccagg agcagctcgg cgatctggat gacctgctcc ttggtgagcg cccggaacac
     2461 gatgatctcg tcgatccggt tgaggaactc gggccggaaa tgggaccgca ggagctgcat
     2521 caggtcctcc ttcagctcct cgaagtcctc gttccgacgg gcgtgcgcct ggattcgttc
     2581 ggcaccgagg ttgctcgtca tgatcacgac cgtgttcttg aagtccaccg tgcgtccctg
     2641 agcatcggtg aggcgtccgt cgtccaggat ctgcaggagc aggttgaaca cgtcggggtg
     2701 cgccttctcg atctcgtcga gcaacaccac cgagtacggc ttacgccgga cggcctcggt
     2761 gagctgtccg gcctcttcga agcccacgta cccgggcggc gccccgacga gccggctcac
     2821 ggtgtgccgc tcctggaact cgctcatgtc gaacctgatc atcaggtcgt cgtcgccgaa
     2881 caggacctcg gcgagcgtcc tcgcgagctc cgtcttaccg acgccggtcg gcccaaggaa
     2941 caggaagctc ccgacggggc gcttcgggtc cccgagcccg gcccggccac ggcgaacggc
     3001 ctcggcgacg gccaccaccg gctcgtcctg tccgacgacg cgctcgtgca gccgctcctc
     3061 gagcttgagg agccggtctc gctcctcctg cgtgagctgc gagacgggga tgccggtggc
     3121 acgcgagacc acctcggcga tgtcctcgac ggtgacctcc ggcgcccgct ggcgggggtg
     3181 cttctgcccg gatagcgcat cctgcttctc ctgcagggtc gtcttcagtt cgttcgcctt
     3241 ggcgtagtcc tccgcaccca ccgcctgatc gcgttcacgc tcgaggcggc cgatctcctc
     3301 ctccatcgcc ctcgtatcca cgggcttggt cttggcccgc aggcggacgc gtgcggaggc
     3361 ctgatcgatg aggtcgatcg ctttatccgg gaggaaccgg tcgccgatgt agcggtcgga
     3421 gagctcggcg gccgcgacga tcgcctcgtc gg
//
LOCUS       DASUYD010000048         3893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_685410, whole genome shotgun sequence.
ACCESSION   DASUYD010000048 DASUYD010000000
VERSION     DASUYD010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3893
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_685410"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF195_02750"
     CDS             complement(<1..349)
                     /locus_tag="VF195_02750"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6579775.1"
                     /translation="MDGKSSERFETVVIGSGQAGLSTGYYLKEAGRSFLIVDSHDRVG
                     GSWLSRYDSLKLFTPPWAVKLPGWHLPRKGGLCPTKDEMIDYLRAYASRFDLPVRTGL
                     HVDRISRESDGYVL"
     gene            1087..2421
                     /locus_tag="VF195_02755"
     CDS             1087..2421
                     /locus_tag="VF195_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6579776.1"
                     /translation="MIPETRYAKTVDGVHIAYQAVGDGAVDIVAVIGWTTNIEAMWEE
                     PSLARWLTRLSTFGRLVIFDKRGVGLSDRVPESQLPTLETRMDDVRAVMDAAGCERAV
                     VFGISEGGPMSIAFAATYPERTIALVLFGTGTTWEVGDSAELAAYIDDVDRSWGTEEF
                     ARQELRDWAAPSLAEDERMVAWLASYLRRSASPGSAIALERMNRGIDVTHILPAIHVP
                     TLVVSRTEDRAFTSQQGRAIAEGIPGARFIEHPGVDHFFWVGEGDRLLEEVHRFVNEI
                     RVDEAELDRVLATVMFTDIVGSTKKAAELGDHGWLALMGEHDAKVRVQLGRFRGREVD
                     TAGDGFLATFDGPIRAVRCAMAVRDSVRDLGIELRAGLHTGEVELDGEKVRGIAVHIG
                     SRIAGLAGPSEILVSSTVKDLIAGSAVAFADRGQHGLKGVPGEWRVYAVAPD"
     gene            complement(2478..>3893)
                     /locus_tag="VF195_02760"
     CDS             complement(2478..>3893)
                     /locus_tag="VF195_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6579777.1"
                     /translation="GNDPRAAFMAGRLAVEHFDRGEPAVAMGWLMKAQRHLSDRPESV
                     EHGFLALTKANVARFQGDLDGSMTLAEEATRIGRRYANPDLLAMAIHTQGLVLVSRGE
                     VRQGIALLDEAMTSVIAGEVSSLFTGVIYCSVLDACLDLTDLGRAGEWNEAALAWCDT
                     LPPDAPFTALCRLNRAQLATLRGAWSEAETEARRVSDDQTHDPAAAARGFYERGEILR
                     RLGDLGAAEEAFGRARELGLEPQPGLALLRLGQGKAEPALAALRLAVDGETQNRPRLA
                     RLLAALVEIAIAAGELEIAKAAGEDLETIAAGFATPALEAAAMTARGALALAEGHGPT
                     ALENLRDACRIWNELRLPYESARARVLYGIALRAAGVHEDAELELRSAISAFERLGAA
                     PDAAATAALLGGDERPRGLTVREVEVLRLVASGKTNRDIAVELVISEHTVARHLQNIF
                     AKLDVASRSAATAFAFEHGLA"
BASE COUNT          632 a         1312 c         1319 g          630 t
ORIGIN      
        1 acaggacata cccgtcgctc tcccgagaga tccgatcgac gtgcaggccc gttcgcacgg
       61 gaagatcgaa cctcgaggcg tacgcccgga ggtagtcgat catctcgtcc ttcgtcgggc
      121 agagcccgcc cttccggggg agatgccaac cggggagctt gaccgcccag ggcggtgtga
      181 acagcttgag cgagtcgtac cgcgagagcc acgagccgcc gacccggtcg tgcgagtcca
      241 cgatcaggaa cgacctgccc gcctccttga ggtagtaccc ggtcgacaga cccgcctgcc
      301 ccgatccgat gaccaccgtc tcgaaccgct ccgaactctt gccgtccatg gctccctcct
      361 tcgagcgcgt cccgatcgcg accgtaagcc ggagacccgc ggcggctcat cgcccgaaac
      421 aaccaactcg gcgagtcgac gtgtggtgag gaataaccac acgagccccg cttcggtcga
      481 gtcggcggcg agctcgtgac gttcggacca ccgatccgat acaaccccaa ccatgcaacc
      541 atcggggcgc gaaagccgtg ctcctcacag gcgtctacgg cttgggcaag actgccgtcg
      601 cggaagagat cgcgagtgtg ctggaggacg gggcatggcc tacgcggcgc tggagctcga
      661 ttgattgggg ttgagtcgac gcccggctgg gacgacgatg cggcaggaca tcggatgatg
      721 ctgaagaacc tgcaggacgt cgtcggcaac ttacctcgcc gccgatatcg gtctcttcgt
      781 gctcgctctc tcgatcgtga cgcaaccgga gctggatgcc atcaggttct caacgaccgg
      841 tccatccgtg aagtggcgat ggacgtcatg gcccggctga actgggttgg gtcaaccaac
      901 cggcgaaagc cgtcctgaag tcgcttggtg gtctggattc atccgccaac gctcctgcac
      961 cagagcgatc ccgtcgctcg agccgctggt gactctaggc ggatcgcgac ggcaggcgtg
     1021 cgggatgcat gtcccgggcg ggacgcttcg cttgctggcc agctgaccca ggcggagact
     1081 agcggcgtga tccccgagac ccgctacgcc aagaccgtgg acggcgttca catcgcctac
     1141 caggcagtcg gcgatggagc cgtcgatatc gtcgcggtga tcgggtggac cacgaacatc
     1201 gaggccatgt gggaggaacc cagcctcgct cgatggctca ctcgactctc gacgtttggc
     1261 cggctggtca tcttcgacaa gcgaggggtc ggactctcgg accgcgtccc cgagtcgcag
     1321 ctccccaccc tcgagacgcg gatggacgac gtgcgagccg tgatggatgc ggccggatgc
     1381 gagcgagccg tcgtgttcgg catctcggag ggcggaccca tgtccatcgc gttcgctgcg
     1441 acctatccgg aacggacgat cgctctcgtc ctattcggca ccggcacgac ctgggaagta
     1501 ggcgattcgg ccgagttggc tgcgtacatc gacgacgtgg atcgatcctg gggaaccgag
     1561 gagttcgcgc gtcaggagct ccgtgactgg gccgctccga gcctcgccga ggacgagcgg
     1621 atggtggcct ggcttgcctc gtacctccgc cgctcggcga gtccgggttc agcgatcgct
     1681 ctcgagagga tgaaccgggg catcgatgtg acccacatcc ttcccgcgat ccacgttccg
     1741 acgctcgtcg tctctcggac cgaagatagg gcattcacct cgcagcaggg tcgggcgata
     1801 gccgaaggga tccctggagc tcgcttcatc gagcacccgg gagtcgatca cttcttctgg
     1861 gtcggcgagg gggaccggct gctcgaggag gtccaccgat tcgtgaacga gatccgcgtc
     1921 gacgaggccg aactcgatcg cgtccttgcg accgtgatgt tcaccgacat cgtcggctcg
     1981 acgaagaagg cggccgagct cggagaccac gggtggctag ctctcatggg agagcacgac
     2041 gcgaaggtac gcgtccagct cggccggttc cgaggacgcg aggtggacac cgcgggggac
     2101 gggttcctgg ccacgttcga cgggccgatc cgtgcggtgc gatgcgcgat ggcagtgcgc
     2161 gactcggtgc gcgatctcgg tatcgagctg cgtgcggggc tgcacacggg tgaagtcgaa
     2221 ctggacggcg aaaaggtccg cgggatcgcg gtgcacatcg gctcccgtat cgctgggctc
     2281 gccggaccct ccgagatcct cgtgtcctcg acggtgaagg atctcatcgc gggctcggcc
     2341 gtggcgttcg cggaccgagg ccagcacggg ctcaagggcg tgcctggcga gtggcgcgtc
     2401 tacgccgtgg cgccggacta agttcctaga gagggaagct caagcgacat ccgcgaacac
     2461 tcgcgcaggc agcgtgacta cgccaagccg tgctcgaacg cgaacgccgt cgcggccgag
     2521 cgcgatgcaa cgtcgagctt ggcgaagatg ttctgcaggt gtcgcgccac cgtgtgctcg
     2581 ctgatcacca gctcgacggc gatgtcgcgg ttggtcttcc ccgacgcgac cagccgcaag
     2641 acctcgacct cgcgcaccgt gagccctcgc gggcgctcgt ccccgccgag gagggcggcc
     2701 gtggccgccg cgtccggcgc ggcccccagg cgttcgaacg ccgagatcgc ggagcgaagt
     2761 tcgagctccg cgtcctcgtg gacgccggcg gcgcgcaggg cgatcccgta caggacccgt
     2821 gcccgggcgc tctcgtacgg cagcctcagc tcgttccaga tccggcacgc gtccctgagg
     2881 ttctcgagcg ctgtggggcc gtgaccttcg gcgagcgcca gggctccccg tgccgtcatc
     2941 gccgcggcct ccaacgccgg cgtggcgaac ccagcggcga tcgtctcgag gtcctcgccg
     3001 gctgctttcg cgatctcgag ttctcccgcc gcgatcgcga tctccaccag cgcggccagc
     3061 aggcgcgcga gccgcggccg gttctgcgtc tctccgtcga ccgcgaggcg gagggccgcg
     3121 agcgcgggtt ccgccttgcc ctggccgagt cgaaggagcg cgagcccggg ctgcggctcg
     3181 aggcccagct cgcgggcacg gccgaaggct tcctcggcgg cccccaggtc accgagccgg
     3241 cgcaggatct cacccctctc gtagaaaccc ctcgccgcgg cggccgggtc gtgcgtctga
     3301 tcgtcggaga cgcgacgggc ctcggtctcg gcttccgacc acgcgccgcg aagtgtcgcc
     3361 agctgagctc gattgagacg gcacagggcc gtgaacggag catccggcgg caacgtgtcg
     3421 caccatgcga gtgccgcctc gttccattcg cccgctcgtc ccaggtctgt cagatcgaga
     3481 caagcgtcca ggacgctgca gtagatcacc ccggtgaaga gcgagctcac ctctccggcg
     3541 atgaccgagg tcatcgcctc gtccaagagc gcgatcccct gccgaacctc tcctcgggac
     3601 acgagcacga gtccctgggt gtggattgcc atcgcgagga ggtcggggtt ggcgtaccgc
     3661 cgaccgatcc gggtggcttc ttcggcgagc gtcatcgatc catccagatc accctggaag
     3721 cgcgcgacgt tggccttggt tagcgcgagg aagccgtgct cgacggactc cggccgatcg
     3781 ctgagatggc gttgcgcctt catgagccag cccatcgcca ccgccggttc gccgcggtcg
     3841 aagtgctcga cggcgagacg accggccatg aacgccgccc tcggatcgtt ccc
//
LOCUS       DASUYD010000049         4707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_906536, whole genome shotgun sequence.
ACCESSION   DASUYD010000049 DASUYD010000000
VERSION     DASUYD010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4707
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_906536"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /gene="ahcY"
                     /locus_tag="VF195_02765"
     CDS             complement(<1..1095)
                     /gene="ahcY"
                     /locus_tag="VF195_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX6579778.1"
                     /translation="MGHDIRDGGLAESGRGRIDWAAGEMPVIAQIRERFDKEKPLQGV
                     RVAACLHVTTETANLMETLAAGGAEVALCASNPLSTQDDVAASLVEQSGIDVYAIKGE
                     DTDMFFKHINACLDLKPHVTMDDGADMVSVLHKQRVDQIDEIMGGTEETTTGVIRLRA
                     MASDRALRYPIVSVNDANTKHLFDNRFGTGQSTIDAIMRATNRLLAGRAVVVCGYGMC
                     GRGVASRARGMGAHVIVVEVDPTAALEAVMEGFRVMPLLEAARVGDVFVTVTGDKGVI
                     RSEHMERMKDGAILANSGHFDVEIDKAALAALSEGRVRRIREFVDEHTLADGRRLNLL
                     GEGRLVNLAAAEGHPAAVMDMSFANQALCVE"
     gene            complement(1109..1954)
                     /locus_tag="VF195_02770"
     CDS             complement(1109..1954)
                     /locus_tag="VF195_02770"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX6579779.1"
                     /translation="MTAERVPSPGDGVAERIVGVIRERSDLRPVAGVVLGSGLGDSIS
                     AAWDRAGSGEPIEIAYADLPGFPPPTVPGHEGTLWLGTIAGQPIAIFRGRIHFYEGHP
                     MALASITSRISHGLGARTMVLSAAVGGLDPALPAASMVVVRDHLNLMGTNPLTGWRYE
                     DGSPAFVDVSGVYDPQLADAALRALRDHGIAVADGVYAAVAGPAYETPAETEMLRRMG
                     ATVVGMSTVPEAVAARALGMRVLGLSFVTNAAGGAVSHEEVLLASKTAAEAIGLVVAE
                     LADTF"
     gene            complement(1951..3009)
                     /locus_tag="VF195_02775"
     CDS             complement(1951..3009)
                     /locus_tag="VF195_02775"
                     /inference="COORDINATES: protein motif:HMM:NF006426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HEX6579780.1"
                     /translation="MTIDLDDLEAVRAHDPGGMLVAAAQLASHCREGYANGTGAGALP
                     DLVDVRNVVYCGMGGSAVAGDVLRSVFRDRLGVPVEVNRSSELPEHAGPHSLVVACSY
                     SGNTSETLAAFREAMKRGCRSVVITSGGVIADEAAEAGVPVVRVPGGMQPRAALGWLG
                     CTALGALETAGLLPPLAEDMADAANEIEARAATCGAAVPAADNPAKLLAEEIGERVPV
                     IWGAEGIGAVAAARWKTQMNENGKVPSWWSSMSELDHNEVVGWTQPYGRAHAVIALRH
                     DAEDPEMATRFPLSMRIAAEAGATTAEVHATGRSALARLFSLISFGDFVSVYVAIRRG
                     IDPTPVDVITRLKAALVS"
     gene            complement(3006..3200)
                     /locus_tag="VF195_02780"
     CDS             complement(3006..3200)
                     /locus_tag="VF195_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX6579781.1"
                     /translation="MTLDAELLEILVCPNDRGDIDYHEDEQVIVCRTCGYRYPVRDGI
                     PVMLIDEAEKPGGGPGVKNG"
     gene            complement(3212..4600)
                     /gene="manB"
                     /locus_tag="VF195_02785"
     CDS             complement(3212..4600)
                     /gene="manB"
                     /locus_tag="VF195_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222900.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX6579782.1"
                     /translation="MAPVDLSRIFKAYDVRGTVPDQLNADVVRRIGAAFADWSGADEI
                     LVGRDCRLSSPELAAALIEGATSRGVDVVDLGLASTDLLYFASGALDRPGVMITASHN
                     PKDYNGLKFCLARARPVGEESGLGEIRARSEKGDDILGAGNGSVRRRDVLGAFVDHVL
                     SFTDVDRMRPLTVAVDTANGMGGLVVPAVMERLPVTLHHLYAELDGTFPNHPADPIDP
                     ANQRDLKRAVLEHRADVGMAFDGDADRVFLVDERADDVSGSILTALVAAAMLDREPGA
                     QVVHNLICSWTVPEVIEEHGGVPVRTRVGHSFIKQVMAETGAVFGGEHSGHYYFRDNY
                     GADSGLIASVIALGELSDASVPLSGLLGQYRRYASSGEINSRVDEPLARIEDIARALR
                     DGRQDRLDGLTVEFDDWWCNVRPSNTEPLLRLNVEARTEEILAARAAELLALIRGAGE
                     AGGNAPGTNEEG"
BASE COUNT          708 a         1758 c         1526 g          715 t
ORIGIN      
        1 ctccacacag agcgcctggt tcgcgaacga catgtccatc accgcggcgg ggtggccctc
       61 cgcggcagcg aggttcacga gcctcccctc gcccaggagg ttgagccgac ggccgtcggc
      121 caacgtgtgc tcgtccacga actcgcggat ccgccgcacc cgcccttccg agagcgcggc
      181 cagcgcagcc ttgtcgatct ccacgtcgaa gtgccccgag ttcgcgagga tcgcgccgtc
      241 cttcatccgc tccatgtgct cgctacggat cacgcccttg tccccggtca ccgtgacgaa
      301 cacgtcgccc acccgagccg cctcgagcaa cggcatgacg cggaagccct ccatcacggc
      361 ctcgagggcg gccgtcgggt cgacctcgac gacgatcacg tgcgcgccca tcccccgtgc
      421 acgcgacgcc acgccacgcc cgcacatccc gtacccgcac accaccacgg cgcggccggc
      481 cagcagacgg ttcgtcgctc gcatgatcgc gtcgatcgtg gactggccgg tcccgaagcg
      541 attgtcgaac aggtgcttgg tgttggcgtc gttcacgctc acgatcgggt accggagcgc
      601 gcggtcggag gccatcgccc ggaggcggat cacgcccgtg gtcgtctcct ccgtcccgcc
      661 catgatctcg tcgatctggt ccacgcgctg cttgtgcagc acgctcacca tgtccgcgcc
      721 gtcgtccatc gtgacgtgcg gcttgaggtc gaggcacgcg ttgatgtgct tgaagaacat
      781 gtcggtgtcc tcgcctttga tcgcgtagac atcgatgccg ctctgctcga ccagggatgc
      841 ggcgacgtcg tcctgggtcg acagcgggtt ggaggcgcag agcgccacct cggcgccgcc
      901 tgctgccagc gtctccatca ggttcgccgt ctccgtggtg acgtgcagac aggcggcgac
      961 ccgcacgccc tgcaacggct tctccttgtc gaaccgctcc ctgatctgag cgatcacggg
     1021 catctcgccc gcggcccagt cgatcctgcc ccgaccggac tccgcgagcc cgccgtccct
     1081 gatgtcgtgt cccactggat cctctccgtc agaaggtgtc ggcgagctcg gccaccacaa
     1141 ggccgatcgc ctccgccgcc gtcttcgacg cgagcagcac ctcctcgtgg gagacggcgc
     1201 cgcccgcggc gttcgtgacg aagcttagcc cgaggacgcg catgcccagc gcccgggcgg
     1261 cgacggcctc cggcactgtg gacatcccca cgaccgtcgc ccccatccgg cggagcatct
     1321 ccgtctcggc gggggtctcg tacgcgggac ccgctaccgc ggcgtagacg ccgtccgcca
     1381 ccgcgatacc gtggtcccgg agcgcgcgga gcgcggcgtc cgcgagctgt gggtcgtaga
     1441 cccccgagac gtcgacgaac gccggtgagc cgtcctcgta ccgccacccg gtgagcgggt
     1501 tggtccccat caggttgagg tgatcgcgga cgacgaccat ggaggccgcg ggcagcgccg
     1561 gatcgaggcc gccgaccgcc gcagacagca ccatcgtccg tgccccgagg ccgtgagaga
     1621 tccgggaggt gatcgacgcc agcgccatcg ggtgaccttc gtagaagtgg atccggccgc
     1681 ggaagatcgc gatcggctga ccggcgatcg tccccagcca gagcgtgcct tcgtgaccag
     1741 gcaccgtcgg aggcgggaac cccgggaggt cggcgtaggc gatctcgatc ggttcgccag
     1801 atccggcgcg atcccaggcg gccgagatcg agtccccaag gccggagccg aggacgacac
     1861 cggccaccgg tcgcagatcg ctccgctcac ggatcacccc gacgatccgc tccgcgaccc
     1921 cgtccccggg cgacgggacg cgctccgccg tcacgagacg agcgcggcct tcagtcgggt
     1981 gatcacgtcg accggcgtcg gatcgatgcc ccggcggatc gcgacgtaga cgctcacgaa
     2041 gtcgccgaag gagatcagcg agaacaagcg ggcgagagcg ctccgtccgg tcgcgtggac
     2101 ctcggcggtg gtggcgcccg cttccgcggc gatccgcatc gagagcggga agcgcgtggc
     2161 catctccgga tcctcggcgt cgtgccggag cgcgatgacc gcgtgcgccc ggccgtaggg
     2221 ctgcgtccac cccacgacct cgttgtggtc gagctcggac atggatgacc accaggaggg
     2281 caccttgccg ttctcgttca tctgcgtctt ccagcgagcc gccgccacgg ccccgatgcc
     2341 ctccgcgccc cagatcacgg ggacgcgctc gccgatctcc tccgccagga gcttcgccgg
     2401 gttgtcggca gccggtaccg ccgcaccgca cgtcgcggcc cgggcctcga tctcgttggc
     2461 agcgtcggcc atgtcctcgg cgagcggggg cagcaacccc gcggtctcca acgcgccgag
     2521 cgccgtgcac ccgagccaac ccagcgcggc acgtggctgc atgccgcctg gcacgcggac
     2581 gaccggcacg cccgcctcgg ctgcctcgtc cgcgatcacc ccgccggacg tgatcacgac
     2641 cgaacgacag ccacgcttca tcgcctcccg gaacgccgcg agcgtctcgg aggtgttccc
     2701 cgaataggag cacgcgacga cgagcgaatg cgggcctgcg tgctcgggga gctccgacga
     2761 tcgattcacc tcgacgggga cgccgagcct gtcgcggaac accgatcgca gcacatcgcc
     2821 ggcgacagcc gacccgccca tcccgcaata gacgacgttg cgtacgtcca cgaggtccgg
     2881 gagcgcaccc gctcccgtgc cgttcgcata cccctcacgg caatgggagg cgagctgggc
     2941 ggcggccacc agcatccccc cgggatcgtg agctcgaacg gcctcgaggt cgtccaggtc
     3001 gatcgtcacc cgttcttgac tcctgggcca ccaccgggct tctcggcctc gtcgatcagc
     3061 atcaccggga tgccgtcgcg caccgggtag cggtagccgc acgtgcggca cacgatcacc
     3121 tgctcgtcct cgtggtagtc gatgtcaccc cgatcgttgg ggcacaccag gatctccaac
     3181 agctccgcat cgagcgtcac ggctcgtcct ttcatccctc ttcgttggtt cccggagcgt
     3241 tccctccggc ctcgcccgct cctctgatca gggcgaggag ctccgccgct ctggccgcca
     3301 ggatctcttc cgtccgcgcc tccacgttca gccgcagcag cggctccgtg ttcgacggcc
     3361 ggacgttgca ccaccagtcg tcgaactcta ccgtcagtcc gtcgagccgg tcctgccgac
     3421 cgtcgcggag cgcccgcgcg atgtcctcga tcctcgccag cggctcgtcg acgcgggagt
     3481 tgatctctcc ggagctcgcg tagcgccggt actgccccag gagccccgac agcgggaccg
     3541 aggcgtccga gagctccccg agggcgatca ccgacgcgat cagtccggag tcggcgccgt
     3601 agttgtcccg gaagtagtag tgccccgaat gctcgccccc gaacaccgcg cccgtctccg
     3661 ccatcacctg cttgatgaac gagtgcccga cccgggttcg gaccggtaca ccaccgtgct
     3721 cctcgatcac ctcggggacc gtccaggaac agatcaggtt gtggaccacc tgcgcacccg
     3781 gttcacggtc cagcatcgcc gccgccacga gcgcggtgag gatcgatccg ctgacgtcgt
     3841 ccgcccgctc gtccaccagg aagacgcggt ccgcgtcgcc gtcgaacgcc atccccacgt
     3901 ccgcacggtg ctcgaggacg gctcgcttca ggtctcgttg gttcgccgga tcgatcgggt
     3961 ccgcggggtg attcgggaat gtcccgtcga gctccgcgta caggtgatgc agggtgacgg
     4021 gcagccgctc catcaccgcc ggcacgacga gaccgcccat cccgttggcc gtgtcgaccg
     4081 cgaccgtcag cgggcgcatc cgatcgacgt ccgtgaacga gagcacgtga tcgacgaagg
     4141 cgcccaggac gtcgcgtcgg cggaccgagc cgttgcccgc cccgaggatg tcgtcaccct
     4201 tctcgctgcg cgcccggatc tcaccgaggc ccgactcttc tcccaccggc ctcgctcgcg
     4261 cgagacagaa cttgagtccg ttgtagtcct tgggattgtg gctcgcggtg atcatcacgc
     4321 cgggccggtc gagggctccg gaggcgaagt acaagaggtc ggtcgacgcg agtcccaggt
     4381 cgacgacgtc gactccgcgc gacgtggcgc cctcgatcaa agcggctgcc agctccggag
     4441 aggagaggcg gcaatcgcgg ccgacgagga tctcgtccgc tccgctccag tccgcgaagg
     4501 ccgcgccgat gcgcctgaca acgtcggcgt tcagctgatc ggggaccgtg ccgcgaacgt
     4561 cgtacgcctt gaagatgcgc gagaggtcca ccggcgccat cctacggacg gcgcgcggcg
     4621 ggcgtcaggc taggtccgga cgacggtctc gatcacgtcg gagagcacga tctgctcgcc
     4681 gtcgcgatgc caccacttcg cctcaca
//
LOCUS       DASUYD010000050         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1127141, whole genome shotgun sequence.
ACCESSION   DASUYD010000050 DASUYD010000000
VERSION     DASUYD010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127141"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF195_02790"
     CDS             complement(<1..223)
                     /locus_tag="VF195_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579783.1"
                     /translation="MFTSWKRVAIGALVLVVVVVGLFIVLPLIADWQTVNPRHTSRFP
                     LTLPAVYAAIGWTNTPSHGVPEPTWSPFRA"
     gene            complement(321..752)
                     /locus_tag="VF195_02795"
     CDS             complement(321..752)
                     /locus_tag="VF195_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX6579784.1"
                     /translation="MAEPSLYERLGGVFAIAAVIDHFSDAVVQNSIVGQASENPELRE
                     WHTNQLGRLPGLKFMRTLWVCNVAGGPFTFAATKPGSTPLGLEEAHRDLHISPEEFDE
                     VAAELGRSLDHFGVPPREKDEVLAAFAAHKGEVTEGSMASA"
     gene            complement(948..>2015)
                     /locus_tag="VF195_02800"
     CDS             complement(948..>2015)
                     /locus_tag="VF195_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579785.1"
                     /translation="RVFLSALAVGGAYLALRQWGRPDRRPSGPSQTPPTPSSSPASDE
                     GLYGIDNWPGLDWHLFSDTSVWNTPLDKSKVDPNSAVMIRTLMSDGLPRDITADVRSM
                     WGFPFYFARAQDPVYEIVISGTSAPFAREINGLSVHCPVGVETSSGSDGVFRLVEQTD
                     GYTYHFQRAFVDDTARVIHAWRSYRLESDGPGFHSIEEPPTGLEPIRPEELAAGFVRH
                     TVGMHARCLSGHNVAPYDLSGTEGVPCDPINDPTTRLSMGNVVFVDMTVAEVEALNIP
                     TYQKAILKGLAVHGALVGFNGSHNWTLTYEAPQDRTAFGRPDPYEAAGLPSTLSIANA
                     LDAVGGWGAKLKVLAPFRRPV"
BASE COUNT          341 a          674 c          668 g          332 t
ORIGIN      
        1 gtgcgcggaa cggtgaccac gtaggttccg ggacgccgtg cgacggagta ttcgtccacc
       61 cgatcgcagc gtagaccgcc ggtagcgtca ggggaaatcg agatgtgtgc cgagggttca
      121 cggtctgcca gtcggcgatc aacggcagga cgatgaagag gccgaccacc acgacgacga
      181 ggaccagagc gccgatcgct acccgcttcc atgacgtgaa catcgggtag ggctcgggcc
      241 accgttcggg tcgaacagcc ccatcgtgtt ccgaggatac ggtggcggat gacgctgcga
      301 tgcggatgaa ggcacagccc tcaggccgag gccatcgatc cctcggtcac ttcccctttg
      361 tgcgccgcga aggccgccag gacctcgtcc ttctcacgcg gagggacccc gaagtgatcg
      421 agcgaccgcc ccagctcagc cgcgacctca tcgaactcct cgggggagat gtgaaggtcg
      481 cgatgggcct cttcgagccc gagaggggtg ctccctggct tcgtggcagc gaacgtgaac
      541 ggtccaccgg cgacgttgca gacccacagc gtgcgcatga acttgagtcc cggcagtctc
      601 ccgagttggt tggtgtgcca ctcacgaagc tcagggttct ccgatgcttg gccgacgatg
      661 gagttctgga ccaccgcatc gctgaagtgg tcgatcaccg cggcgatcgc gaacacgcca
      721 cccaatcgtt cgtacaggct cggctcagcc atgcctcgtc ccctcccctt ctctccacct
      781 aaccgcgctg caggatgcgt ctggagggtc tacgggtggc acctgggatc ggttcaggtc
      841 tcgaggccga ccggcgccta caagaggaat ggtggcccgc cacggagccc cagatgatcg
      901 cgatgacccg cgtaccgccc ggtggaacgc ggaggtccgt tgaggggtca gaccggccga
      961 cggaagggtg cgagcacctt cagcttcgcg ccccacccgc cgaccgcatc gagcgcgttg
     1021 gcgatcgaca gcgtcgaggg caacccggct gcctcgtagg gatccggcct accgaaggcg
     1081 gtccggtcct gcggggcttc gtaggtcagc gtccagttgt gcgagccgtt gaagcccacg
     1141 agggctccgt ggaccgccag tcctttcagg atcgccttct ggtacgtcgg gatgttcagc
     1201 gcctcgacct cggcgaccgt catgtccacg aacacgacgt tgcccatgga cagccgcgtc
     1261 gtcgggtcgt tgatcggatc gcacgggaca ccttcggtgc ccgagaggtc gtacggagcg
     1321 acgttgtgac ccgacaggca cctcgcgtgc atccccaccg tgtgccggac gaaccccgcg
     1381 gcgagttcct ccggccggat cggctccagg ccggtcgggg gctcctcgat gctgtggaac
     1441 cccggcccgt ccgattcgag tcggtacgaa cgccacgcgt ggatcacccg agcggtgtcg
     1501 tccacgaacg cccgttggaa gtggtacgtg tagccatcgg tctgctcgac cagccggaac
     1561 acgccgtcgg atccgctcga ggtctcgacc ccgaccgggc agtgcacgga gagcccgttg
     1621 atctcccgcg cgaacggcgc gctcgtcccc gatatgacga tctcgtacac ggggtcctgc
     1681 gcccgcgcga agtagaacgg gaatccccac atcgatcgga catccgccgt gatgtccctg
     1741 gggagcccgt cggacatgag cgtgcggatc atcaccgcgg agttggggtc gaccttcgac
     1801 ttgtccagcg gcgtgttcca tacgctcgtg tcggagaaca ggtgccagtc gagtccgggc
     1861 cagttgtcga tgccgtacag gccttcatcc gaggccggcg acgaacttgg ggttggcggc
     1921 gtctggctcg gcccagaagg acgccggtcc ggcctgcccc actgacgcag cgccaggtag
     1981 gcgcccccga ccgccaaggc gctcaggaac accct
//
LOCUS       DASUYD010000051         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_25031, whole genome shotgun sequence.
ACCESSION   DASUYD010000051 DASUYD010000000
VERSION     DASUYD010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25031"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..759)
                     /locus_tag="VF195_02805"
     CDS             complement(4..759)
                     /locus_tag="VF195_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6579786.1"
                     /translation="MTSNGSALAVVDLHKSFGKLEVLRGIDLEVREHEVVCVIGASGS
                     GKSTLLRCINLLEPVNAGRVLIEGEEITARGVDVNRIRQRVGIVFQAFNLFPHMTVIR
                     NVTLGPIEALGSSKGDAEALATELLERFGLADKRDDYPDRLSGGQQQRVAIVRALAMK
                     PDVMLLDEVTSALDPELVAEVLNVIRELAAGGMTMVIATHEMGFARDIAHRVCFLDEG
                     VILEEGAPEQIFSSPREERTQRFLQSIIDAGRM"
     gene            complement(756..1655)
                     /locus_tag="VF195_02810"
     CDS             complement(756..1655)
                     /locus_tag="VF195_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6579787.1"
                     /translation="MGGGGSPELHQYQPGDPRLPVGQRTSRILGPLRAEGARGVAIAL
                     LSTVVVLVLIAWAVTSSPNWPEVKQAFFDPEEFKRAFPDIARAFLRNIQYFLIAEVFI
                     LAWALVLAVLRSLQGPVFFPLRALAIVYTDLFRGIPTVLVVYLLGFGIPSLGLPGVPS
                     GAAFWGVVGLILSYSAYVAEVYRAGIESVHPSQAAAARSLGLSRWKTLRFVVIPQAVR
                     RVIPPLLNDFIGLQKDTALVAFVVAVPEAFKQGQIYQAADFNFTPLLAVALMFLLITV
                     PLTRFTDWLLERQRRRRQAGAAA"
     gene            complement(1674..2618)
                     /locus_tag="VF195_02815"
     CDS             complement(1674..2618)
                     /locus_tag="VF195_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6579788.1"
                     /translation="MRKTFASLVVGSLLFAACAEDNPTIGGGATSTTSTGGTGATAPA
                     TAAECAAANQDTLVASGVLTVGTDNPAYPPWFSGGETDAHSEWKIDDPYTGEGYEAAT
                     AYAVAEAMGFSQDQVAWVVAPFNQTYKPGDKDYDFAIEQISYSDKRDEAVDFTESYYD
                     VQQALVAVEGSPIAGASSISDLAGYTIAAPIGTTSYDYIVENIPDATAGSYTTLADTV
                     AALNAGQVDGIVVDSPTAFYLADPFVQEVKDGVVVGLFPAGGEQEYFGLTFETGNSLV
                     DCANLALKEIKADGTLDAIHQEWLVEKTNVGEVPEFTT"
     gene            complement(2698..3474)
                     /locus_tag="VF195_02820"
     CDS             complement(2698..3474)
                     /locus_tag="VF195_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6579789.1"
                     /translation="MSDAGLLALQIRTTNKAFWRNPASAFFTFAFPLMFLVIFTALLG
                     SGSVEVAPGVVIQQDSYYVAAMAAFAVITATYTNLAISISFTRDAGILKRTRGSPLPA
                     GIYLGGRVTHAILISILLVVITTAFGVAFYQAEVPTGVLLWELLATVVVGAMAFSALG
                     LATTALVPNADAAPAVVNAIILPLLFLSGIFIPLGEDAPTWMKVIGDVFPVKHFAKAM
                     LGSFYGPPFPFEWSDVLIVAAWGVVGLALAIRFFSWEPRK"
BASE COUNT          625 a         1175 c         1162 g          554 t
ORIGIN      
        1 ccatcacatc cgtccggcgt cgatgatgct ctggaggaac cgctgggtcc gctcctcgcg
       61 cggcgacgag aagatctgct ccggcgcacc ctcctcgagg atcaccccct cgtcgaggaa
      121 gcagacccgg tgagcgatgt cccgagcgaa ccccatctcg tgggtggcga tcaccatcgt
      181 catgccgccg gcggcgagct cccggatcac gttcaggacc tctgcgacga gctcggggtc
      241 cagggcgctc gtgacctcgt cgaggagcat gacgtcgggc ttcatcgcga gcgcccggac
      301 gatcgccacg cgctgctgct gcccgcccga gagccgatcc gggtagtcgt cgcgtttgtc
      361 ggcgagcccg aaccgctcga gcaactccgt ggcgagcgcc tcggcgtccc ccttcgacga
      421 tccgagagcc tcgatcggcc ccagcgtgac gttgcggatc acggtcatat gcgggaacag
      481 gttgaacgcc tggaagacga tccccacgcg ctgccggatc cggttcacgt cgaccccgcg
      541 ggccgtgatc tcctcgccct cgatcaacac ccgcccggcg ttcacgggtt cgagcaggtt
      601 gatgcaccgg agcagggtcg acttgcccga acccgacgcg ccgatcacgc acacgacctc
      661 gtgctctcgt acctcgaggt cgatgccccg cagcacctcc agcttgccga acgacttgtg
      721 cagatcgacg accgcaaggg cggaaccgtt gctcgtcatg cggccgcccc cgcctgacgg
      781 cgccggcgct ggcgttcgag gagccaatcc gtgaagcgcg tgaggggcac cgtgatcagc
      841 aggaacatca gtgcgacagc taggagtggt gtgaagttga agtcggcggc ctggtagatc
      901 tggccctgct tgaaggcctc ggggaccgcg accacgaacg ccacgagcgc ggtgtctttc
      961 tgcaggccga tgaagtcgtt caacaggggc gggatcacgc gccgaacggc ctgagggata
     1021 acgacgaagc ggagcgtctt ccagcgcgag agcccgaggg accgggcggc ggcggcctgg
     1081 ctcgggtgca cagactcgat cccggctcgg tagacctctg ccacataagc ggagtaggag
     1141 aggatcaagc cgacgacgcc ccagaacgca gcgcccgagg ggacgcccgg cagaccgagg
     1201 ctggggatcc cgaaccccag caggtacacg acgaggacgg tcgggatgcc tcggaacagg
     1261 tcggtgtaga cgatcgcgag cgctcggagc gggaagaaca ccggaccctg caggctgcgc
     1321 agaacggcta gcacgagagc ccacgccagg atgaatacct ccgcaatgag gaagtattgg
     1381 atgttgcgca ggaacgctcg tgcgatgtcg gggaacgccc gcttaaactc ctcggggtcg
     1441 aagaacgcct gtttgacctc gggccagttt ggcgacgacg tcaccgccca cgcgatcagg
     1501 acgaggacga cgaccgtgct taagagcgcg atcgcgacgc cccgtgcgcc ctcggcgcgg
     1561 agcggtccga ggatccgcga cgttcgctgc cccacgggca gcctcgggtc acccggctgg
     1621 tactgatgca gctcggggga tccgccgcct cccatatgcc gccgaggcca ccgctacgtc
     1681 gtgaactcgg ggacctcgcc gacgttggtc ttctcgacga gccattcctg gtggatggcg
     1741 tcgagcgttc cgtccgcctt gatctccttg agcgcgaggt tcgcgcagtc gaccagcgag
     1801 ttgccggtct cgaacgtgag cccgaagtat tcctgctcgc ccccggcagg gaacagaccg
     1861 acgaccacgc catccttcac ctcttgcacg aagggatccg cgagatagaa ggcggtcggc
     1921 gaatcgacca cgatcccatc gacctgcccc gcgttcaatg cggccacggt gtccgccagc
     1981 gtcgtgtaag accccgcagt ggcgtccggg atattctcca cgatgtagtc gtagctggtc
     2041 gtaccgatcg gtgccgcgat cgtgtacccg gccaggtcgc tgatgctgct cgcgcccgcg
     2101 atgggtgacc cctccaccgc cacgagcgcc tgctgaacgt cgtagtagct ctcggtgaag
     2161 tcgacggcct cgtcgcgttt gtcggagtag gagatttgct cgatcgcgaa gtcgtaatcc
     2221 ttgtcacccg gcttgtacgt ctggttgaaa ggagcgacga cccatgcaac ctggtcctgc
     2281 gagaagccca tcgcctcggc cacagcgtac gccgtcgctg cctcgtatcc ttcgcccgtg
     2341 tagggatcgt cgatcttcca ctcgctgtgg gcgtcagtct ccccacccga gaaccacggc
     2401 gggtaggcgg gattgtccgt gccgaccgtc aacacgccgg acgcgaccag cgtgtcctgg
     2461 ttcgcggcag cacactccgc cgcggtcgct ggggccgttg cgcccgttcc tcccgtggag
     2521 gttgtactgg tagcgccgcc gccgatcgtc gggttgtctt ccgcgcacgc ggcgaacagc
     2581 aaggaaccga caacgaggga ggcgaacgtc tttcgcatcg tgggacccct tggccgacct
     2641 ggtggtccgc ggagactagc acacgcctgt tctacgaagt ccggccggcg tccggcctca
     2701 cttgcgcggc tcccacgaga agaagcggat cgcgagcgcg agcccgacga ccccccaggc
     2761 ggccacgatg agcacgtccg accactcgaa cgggaagggc gggccgtaga acgatcccag
     2821 catcgccttc gcgaaatgct tcaccgggaa aacgtcgccg atcaccttca tccacgtggg
     2881 agcgtcctct ccgagcggga tgaagatccc cgacaggaac agcagcggca ggatgatcgc
     2941 gttgaccacg gcgggggccg cgtcggcgtt cggaacgagg gcggtcgtcg cgaggccgag
     3001 cgccgagaac gccatcgcgc ccaccacgac cgtggcgagc agctcccaca agagcacgcc
     3061 tgtcgggacc tcggcttggt agaacgcgac gccgaacgcc gtcgtgatga cgacgagcag
     3121 gatcgagatc aagatggcgt gcgtcacgcg tccaccgagg tagatcccgg caggcagcgg
     3181 gctcccgcgg gtccgcttca ggatccccgc gtcccgtgtg aaggagatgc tgatcgccag
     3241 gttcgtgtac gtggcggtga tcaccgcgaa cgccgccatc gccgctacgt agtagctgtc
     3301 ctgttggatc acgacaccgg gcgcgacttc gacgctcccg ctcccgagca gggcggtgaa
     3361 gatcacgagg aacatcaagg ggaacgcgaa ggtgaagaag gccgaggcgg ggttgcgcca
     3421 gaacgccttg ttcgtcgttc ggatctggag cgcgagaagg ccggcgtcgc tcactcggct
     3481 cccgcctctc cttgagtgag ctcgaggtac acatcc
//
LOCUS       DASUYD010000052         5090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_686959, whole genome shotgun sequence.
ACCESSION   DASUYD010000052 DASUYD010000000
VERSION     DASUYD010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_686959"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VF195_02825"
     CDS             complement(<1..518)
                     /locus_tag="VF195_02825"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6579790.1"
                     /translation="MGTRDEAADSEGGSGWGRARNGAAHHVHIRSTASTHGRGSLRTV
                     LAYISQKVVTGFHLGPFMIRPHGLLIAVGFLAGARLMQRYSRPRGISDDSLWRILGWS
                     LAGGLVGMRVAWILGHSGEMDSLLDTIAVWNGGMSLLGGLIGAVLVGGFIARREKLTV
                     LPLLDMAAPGIAL"
     gene            633..827
                     /locus_tag="VF195_02830"
     CDS             633..827
                     /locus_tag="VF195_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579791.1"
                     /translation="MGRRLACGVMTLAAAGLVVAVLLGAPLVSILLAGLLLLCPLLLW
                     APFRYQDRSFGALHPGRHPR"
     gene            824..1075
                     /locus_tag="VF195_02835"
     CDS             824..1075
                     /locus_tag="VF195_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEX6579792.1"
                     /translation="MTDVVVLSSPACHLCEDALESLAELRREFPIRVREIGMQTPEGR
                     ELLRIHRPAMPPAVLVDGSLFSVGRLPRKKLRKALERAA"
     gene            1075..2100
                     /locus_tag="VF195_02840"
     CDS             1075..2100
                     /locus_tag="VF195_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX6579793.1"
                     /translation="MGGLLTTGSILAAFFAGAVALFSPCCIVFLFPAYLASAVKARRW
                     RLLPLTLVFAAGIALVLVPITLGIGMVATSLARYHAPLYVAGGLLMLGLAVLALSGHS
                     WNMPSFIRAPDVQRSDAPGVFALGVFSGIASSCCAPVLAGVMTLSVLSSSTVGAATLG
                     LAYVFGMVFPLVLMALLWDRLHLGERRLFRAKPVRLRIAGRTLHTNTLNIAVAVAFAL
                     MGAAVLALGITGNETTAAPMQVAVGRWLSSAFEGVVAFFDPVPEPILGLGLLALALAI
                     FVVAFRGRMAPQQEGVSHEHEDPWEDEEQLDEDATASVGAAVGGAVGGAARSSSDPSR
                     SKPPACH"
     gene            2165..2647
                     /locus_tag="VF195_02845"
     CDS             2165..2647
                     /locus_tag="VF195_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6579794.1"
                     /translation="MVLGGRSPEGGSAGGRATNFTLASTAGGSVSLADYRGKDVLLYF
                     NEGVGCDACFYQTAELEKKADELANAGLTVLPIVMNPVADAARELQRFGVSTPYLIDA
                     DGDVSRAYGMLGKGMHANLPGHGFVLIDGSGHVRWKMEYPSMFVSADDLLAAVEPYLS
                     "
     gene            2768..3457
                     /locus_tag="VF195_02850"
     CDS             2768..3457
                     /locus_tag="VF195_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009278301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579795.1"
                     /translation="MATILVVDDERKIRDLIRSYLEREGYSVLVADTGQRALETLERA
                     SPDLLVLDLMLPDLSGEEIARAVRGHSSLPIIMLTAKAAEDDRVTGLRLGADDYLVKP
                     FSPRELVARVEAVLRRSGESAAPSVSFDGGAMAIDRDAREVRLQGELVELTKSEFDLL
                     HALASHPGRVFSRFELITKVQGYDYEGYERTMDAHVKNLRKKLREDPKHPRYVMTVTG
                     VGYKFGVKSDA"
     gene            3450..4868
                     /locus_tag="VF195_02855"
     CDS             3450..4868
                     /locus_tag="VF195_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579796.1"
                     /translation="MRRSYAFRLAAAFAGIGIAAAALTAIMVNVSFGTRFTSYLQDQQ
                     GARQQQLIAGLTDSYRRSAGWDATDLRSLQSLALMDGTVRLVDLQGNTVWDASTGVSA
                     EMAALHRQMMGSGPLGPEQRLPVRVDGRIVGTAIVRLPEVGLLAQDVSFRSSVNRLLL
                     FGGMLAGLAALLLGIVLARRATAPASELTRAARAQAAGERSERVEFEGPDEFGEMARA
                     FNSMADTIEEEDRLRRAFAADVAHELRTPLAILRTQMEGIQDGVVEPTSPALGSLLDE
                     TLRLSRLVADLESLASADAAGFSLLRQRVDLRPLLAGAAQEFAGPYEAEDVALHTDLY
                     DVQILADPTRIRQVVTNLLSNALKFTPSSGEVALTLQAQGDRAVIRVSDTGPGIPPDE
                     IPHVFDRFFRGRGVRAGGSGIGLTVARELVHAHGGDIRVTSEPGQGATFAVFLPIVSS
                     GARAPFTASSHVTASVGAERRR"
     gene            4871..>5090
                     /locus_tag="VF195_02860"
     CDS             4871..>5090
                     /locus_tag="VF195_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579797.1"
                     /translation="MKLRWISLGVAIGLALALTVGFAFVSGRSTPAAVAQTGGWAAMD
                     AMHDSPAMQRAHAQMPEELRAQCEAMHEQ"
BASE COUNT          717 a         1780 c         1752 g          841 t
ORIGIN      
        1 agcgcgatcc ccggcgccgc catgtccagg agcggcagca ccgtgagctt ctcccggcgg
       61 gcgatgaacc ccccgaccag caccgcgccg atcaggccgc ccagcaggct catccccccg
      121 ttccagacgg cgatcgtgtc caagagcgag tccatctccc ccgagtgacc gaggatccag
      181 gcgacgcgca tcccgaccag tccgccagcg agggaccatc cgaggatccg ccacagcgag
      241 tcgtcggaga tccctcgcgg gcgtgagtac cgctgcatga gacgggcccc ggcgaggaaa
      301 cccacggcga tcagcaggcc gtggggccgg atcatgaagg gcccgagatg gaaccccgtg
      361 acgaccttct gggagatgta ggcgagcacg gtcctcaggc tgccgcgccc gtgtgttgac
      421 gccgtggagc ggatgtgaac gtggtgtgcg gctccattcc gggcgcgccc ccatccggat
      481 ccgccctcgg agtccgcggc ctcatcgcgc gtgcccacgg tcttcaccgg tccttcacac
      541 ggcttgccga tgcttcgggc tgaatggggg tgagcagcat gacgcgacca acgacagagc
      601 cgcggagcga ccgaaccggg cgggtgggat ccatgggccg gcgcctcgcc tgcggggtga
      661 tgacgctcgc cgcggccggg ttggtggttg ccgtgctcct gggcgctccc ctcgtctcca
      721 tcctcctcgc cgggcttctc cttctgtgcc ccctcctgct atgggctccg ttccgctacc
      781 aggaccgctc gttcggcgcg ctgcatcccg ggagacatcc tcgatgaccg acgtcgtcgt
      841 gctctcctcg cccgcgtgcc atctgtgcga ggacgcgctt gagtccctcg ccgagctccg
      901 acgcgagttc ccgatccggg tgcgcgagat cggcatgcag acgcccgaag ggcgcgagct
      961 cctgcggatc caccgtcccg cgatgccccc agcggtgctc gtcgacggct cgttgttcag
     1021 cgtcgggcgg ctgccccgca agaagctccg gaaggccctg gagcgggccg cctgatgggc
     1081 gggctcctca cgaccggctc gatcctggcc gcgttcttcg ccggcgcggt ggccttgttc
     1141 agcccgtgct gcatcgtgtt cctgttcccc gcgtacctgg cctctgccgt gaaggcccga
     1201 cgatggcggc tcctcccgct cacgctcgtg ttcgcggcgg ggatcgccct ggtcctcgtg
     1261 ccgatcacgc tcggcatcgg gatggtggcg acctcgctcg cccgctacca cgcgccgctg
     1321 tacgtcgcgg gcgggctgct gatgctgggg ctcgcggtcc tggcgctctc gggtcacagc
     1381 tggaacatgc cgtcgttcat ccgcgctccc gacgtgcaac gcagcgatgc gcccggcgtg
     1441 ttcgccctcg gcgtgttctc cgggatcgcc tcctcgtgct gcgcgccggt gctcgccggc
     1501 gtgatgacgc tctcggtgct gtcctcgtca acggtgggcg ctgccaccct cgggctggcg
     1561 tacgtgttcg ggatggtgtt cccgctggtc ctcatggcgc tgctctggga ccgtctccac
     1621 ctcggcgagc gccgcctgtt cagggccaag ccggtgcgcc tgcggatcgc cgggcggacg
     1681 ctccacacca acaccctcaa tatcgccgtt gctgtcgcgt tcgcgctcat gggcgcggcg
     1741 gtgctggcgc tcgggatcac cggcaacgag accaccgccg cgccgatgca ggtcgcggtc
     1801 gggcggtggc tctcgagcgc attcgaaggg gtcgtcgcct tcttcgaccc ggtccccgag
     1861 ccgatcctcg ggctggggct gctcgccctg gcccttgcca tcttcgtcgt cgcgttccgg
     1921 ggtcgcatgg ccccgcagca ggagggagtg agccatgagc acgaagaccc gtgggaagac
     1981 gaagagcagc tggacgaaga cgcgaccgca tccgtcggcg ccgcagtcgg cggcgcggtc
     2041 gggggcgcgg cgcggtccag ttccgacccg tcacggtcga agccgccggc gtgccactag
     2101 gcgcccggtc gcgatcggct ggatcgtatt cggggtcctg gcggccgtcg tcgcggtcgg
     2161 cttcgtggtc ctcggcggca ggtcccccga aggtggatcg gccggtggga gggccacgaa
     2221 cttcacgctg gcctcgaccg ccggaggctc cgtctccctc gcggattacc ggggcaaaga
     2281 cgtgctgctg tacttcaacg agggcgtcgg ttgcgacgcc tgcttctacc agactgccga
     2341 gctcgagaag aaggcggacg aactcgcgaa cgccggcctg acggtgctgc cgatcgtgat
     2401 gaacccggtg gcggatgcgg ctcgcgagct gcagcggttc ggcgtatcga cgccctacct
     2461 gatcgacgcc gacggggacg tctcccgtgc gtacggcatg ctcggcaagg ggatgcacgc
     2521 caatctcccg ggtcacgggt tcgtgctgat cgacggatcg ggccacgttc ggtggaagat
     2581 ggagtacccg tcgatgttcg tctccgccga cgatctcctt gccgccgtgg agccctacct
     2641 gagctgagtg gccggcatcg gagggacgtt ggacccggac ggcaggggac ggaagaccct
     2701 gatcggggct gcgccacgtc cggaagctct cggtacgaca gaccgtagtg ggggcctcga
     2761 catgggaatg gcgaccatcc tcgtcgtcga cgacgagcgc aagatccggg acctcatccg
     2821 ttcgtacctg gagcgggagg gctattccgt cctggtggcc gataccgggc agcgggcact
     2881 ggagaccctg gagcgagcga gccccgatct cctggttctc gacctcatgc tcccggacct
     2941 cagcggggag gagatcgcca gagcagtgcg cggccactcg agcctgccga tcatcatgct
     3001 gaccgcgaag gccgcggagg acgatcgggt gaccggcctg cgcctggggg ccgacgacta
     3061 tctcgtgaag ccgttcagcc ctcgggagct cgtagcccgc gtggaggcgg tcctgcgacg
     3121 ctcgggcgag tccgcggccc cgtccgtctc attcgacggc ggagccatgg cgatcgaccg
     3181 cgacgcccgt gaggtgcggc tgcagggcga gctcgtcgag ctcacgaaga gcgagttcga
     3241 tctcctgcac gccctcgcat cgcaccccgg tcgcgtgttc tcacggttcg agctcatcac
     3301 gaaggtgcag ggctacgact acgagggcta cgagcggacc atggacgccc acgtgaagaa
     3361 cctcaggaag aagctgagag aagaccccaa acatcctcgg tacgtcatga ccgtcaccgg
     3421 cgtgggctac aagttcgggg tcaagtccga tgcgtaggtc ctacgcgttc cgactggcgg
     3481 cggcgttcgc gggcatcggg atcgcggcgg cggcgctcac ggcgatcatg gtcaacgtct
     3541 cgttcgggac caggttcacg tcctacctcc aggaccagca gggcgcccgg cagcaacagt
     3601 tgatcgccgg gctcaccgac tcctaccggc gatccgccgg ctgggatgca accgaccttc
     3661 gctcgctcca atccctggcg ctgatggatg gcacggtgcg actcgtcgat ctgcagggca
     3721 acacggtctg ggatgcgtcc accggggtga gcgctgagat ggcagcgttg caccgtcaga
     3781 tgatgggcag cgggccactc ggccccgaac agcggcttcc ggtccgggtt gacggtcgga
     3841 tcgtcggaac cgcgatcgta aggctccccg aggtcgggct cctcgcccag gacgtctcgt
     3901 tcaggtcttc ggtgaatcgg ctcctgttgt tcggggggat gctcgccggg ctcgcggcgc
     3961 tcctcctggg catcgtgctg gcccgccggg cgacggcgcc ggccagcgaa ctcacccggg
     4021 ccgcccgtgc gcaagccgcc ggggagcgct cggagcgggt cgagttcgag ggtcccgacg
     4081 agttcggcga gatggcccga gccttcaact cgatggccga cacgatcgaa gaggaggacc
     4141 gtttgcgccg cgccttcgcc gccgatgtcg cgcacgagct ccgcacgccg ctcgccatcc
     4201 tgcgcaccca gatggaaggg atccaggacg gcgtcgtcga gccgacgtct ccggctctgg
     4261 ggtccttgct cgacgagact ctccggctct cacgcctggt cgccgacctc gagagcctgg
     4321 cctcagccga cgccgcaggg ttctcgctcc tccgccagcg ggtcgacctc cggccgctgc
     4381 tggcgggggc cgcgcaggag ttcgccggcc cgtacgaggc cgaggacgtg gcgcttcaca
     4441 ccgacctcta cgacgtgcag atcctggcgg accccacccg gatccgccag gtcgtcacca
     4501 acctgctctc gaacgccctc aagttcacgc cgtcaagtgg tgaggtcgct ctcacccttc
     4561 aggcccaggg cgaccgcgcg gtcatccgcg tctccgacac gggccccggt atcccccccg
     4621 atgagatccc ccacgtgttc gaccggttct tccgcggtcg cggcgtccgg gcaggcggat
     4681 ccggtatcgg cctgacggtg gcgcgcgaac tcgtccacgc ccacggaggc gacatccgcg
     4741 tcacgagcga acccgggcag ggcgcgacgt tcgcggtctt cctgcccatc gtttcatccg
     4801 gggcgcgcgc ccccttcacc gcctcttcac acgtcacggc tagcgttggg gcagaaagga
     4861 ggcgttgaac atgaagcttc gctggatctc tctcggagtg gcgatcgggc tcgcccttgc
     4921 gctcacggtg ggcttcgcgt tcgtatcggg ccgttccaca ccggcggctg ttgcgcagac
     4981 gggtgggtgg gcggccatgg atgccatgca cgactccccc gcgatgcaac gggcacacgc
     5041 ccagatgccc gaggagctac gggcccagtg cgaggccatg cacgagcaga
//
LOCUS       DASUYD010000053         2296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_25246, whole genome shotgun sequence.
ACCESSION   DASUYD010000053 DASUYD010000000
VERSION     DASUYD010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25246"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1116
                     /locus_tag="VF195_02865"
     CDS             <1..1116
                     /locus_tag="VF195_02865"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX6579798.1"
                     /translation="RGFRRRGRRSRRRVLLTRLGRMELSLAQVGCRCGRRFAPLLQLL
                     GIDRGSRVSPGLARRAAELATELPFARAAAQLLTETGHGLSPATVRRIVARAGERCDL
                     TLPRSDVGDVPAMLIDGTRVPAGPRHGRARSARGVEVNIACAVMGRDVTGRRPRASME
                     LLGASVAQPWLSLHDVVRSPKAIGIVVTDGDNGIDGLLDRALPEVPRQHCTFHVQHNI
                     RLRLWQDGVAFKDRGALTDRLLAPILTAPTRGTSLRALEESITLADDHDFSYAAQHLR
                     NVGSQLSTWKAVRHSRRPWRMDGRSRPEHTTSLLERTMREVNRRVDPPGNRWLVPGVR
                     SMVHLVLARRFDHPAWRGLWQDAGTVKVWAGLRGSTQ"
     gene            complement(1132..1449)
                     /locus_tag="VF195_02870"
     CDS             complement(1132..1449)
                     /locus_tag="VF195_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579799.1"
                     /translation="MNRNRMVRKAYDCLAEVLNHEPSEAEYQRLLSAAAVRYREVLAS
                     NPDLRAHFNDPLITSPEQRWLLENDDATAGWLMIQTTLEANPKPHVVKEFREWMEEPR
                     QRN"
     gene            complement(1720..2292)
                     /locus_tag="VF195_02875"
     CDS             complement(1720..2292)
                     /locus_tag="VF195_02875"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX6579800.1"
                     /translation="MPKRCEWVPLDQPVYLAYHDEEWGVPVHDDARLFEMLSLEGAQA
                     GLSWSTILNKRDGYRIAFAGFDPAKVARFKPAKVERLLQDPGIVRNRLKVESTVNNAA
                     RVREVQRELGSLDAYLWSFVGGHPLVGRFMRSGDITAETAESKSMSKDLKKRGFRFVG
                     PTVCYAFMQACGLVNDHVAVCFRYRELGGR"
BASE COUNT          345 a          796 c          765 g          390 t
ORIGIN      
        1 cgggggttca ggcggcgggg ccgccgttcc cggcgccggg tcctgctcac ccgtctggga
       61 cggatggagc tgagtctcgc ccaggtgggt tgccggtgcg gtcgccgatt cgcgccgttg
      121 ctccagctcc tcggtatcga tcgcgggagc cgggtgtcgc cgggtcttgc ccggcgggcg
      181 gcggagctcg ccaccgagct ccccttcgcc agggcggcgg cgcagctgct gaccgagacc
      241 ggccacggcc tgtccccggc caccgtccgt cggatcgtgg cccgggccgg cgaacgctgc
      301 gacctcaccc tgccacggag cgacgtcgga gatgtcccgg cgatgctgat cgacggcaca
      361 cgggtgccgg ccggcccccg ccacgggcgt gctcgctcgg ctcgtggagt ggaggtcaac
      421 atcgcgtgcg cggtgatggg tcgagacgtc acggggcgaa ggccgagagc ctcgatggag
      481 ctcctcggcg cgtcggtcgc ccagccgtgg ctcagcctgc acgacgtcgt gcgcagcccc
      541 aaggcgatcg gcatcgtcgt caccgacgga gacaacggga tcgacggcct gctcgatcgg
      601 gcgctgcccg aggtcccccg gcagcactgc acctttcacg tgcagcacaa catccggttg
      661 cgcttgtggc aggacggggt cgctttcaag gaccgggggg ctctgacaga tcggttgctc
      721 gctccgatcc tcactgcccc cacccggggc acgagcctcc gggcgctcga ggagtcgatc
      781 actttggccg acgatcacga cttcagctac gccgcccagc atctgcgcaa cgtcggatca
      841 cagctctcga cgtggaaggc cgtgcgccac tcacgccggc cgtggcggat ggacggacgg
      901 tcccgaccag aacacacgac gagcctgttg gaacgcacca tgcgtgaggt caaccgccgc
      961 gtcgatcccc ccggcaatcg ctggctcgta ccaggggtgc gctcgatggt gcacctggtc
     1021 ctcgcccgtc gcttcgatca ccccgcctgg cgaggactgt ggcaggatgc tgggacggtc
     1081 aaggtgtggg ccgggctccg tgggtcaacg caatgacccg ctatcaatgg atcagttcct
     1141 ttgacgcggc tcctccatcc actcgcggaa ctccttgacg acatgcggtt tcgggtttgc
     1201 ctcgagggtg gtttgaatca tgagccaacc cgccgtcgcg tcgtcgttct caaggagcca
     1261 gcgctgctcc gggctcgtga tcagcgggtc gttgaagtgg gcccggagat cggggttcga
     1321 ggcgaggacc tcgcgatacc tcacggcggc cgcggagagg agccgctgat actccgcctc
     1381 ggacggctcg tggttgagca cctcggcgag gcagtcgtac gccttccgaa ccatcctgtt
     1441 cctgttcatc acggcacctc tctcagcaac tcggggaacc gagattccga tcgcgaagca
     1501 tgccgtcgac ttccgcgggc ctcaccgaga cttgggaagt ggctcgagcc gttcggtcca
     1561 gctgcgagcc tgctcgggct tccgcagccg cttctcttcc gcctcttggg ccacgttccg
     1621 ctgctgttgc tcatcacacc gctcgtcagg ctacgccgcc gagttcgaca tagcggaagc
     1681 aggacgccaa gtgttcgaga tcacggttga ggagcgcggc tagcggccgc ccagctcccg
     1741 gtacctgaag catactgcta cgtggtcgtt cacgagcccg cacgcctgca tgaaggcgta
     1801 gcacacggtc ggcccgacga atcggaaccc gcgcttcttc agatccttgc tcatcgactt
     1861 cgactccgcg gtctcggccg tgatgtcgcc cgaacgcatg aaccgcccga cgagcggatg
     1921 gccgccgacg aacgaccaca ggtaggcgtc gagcgaaccg agctcccgct ggacctcgcg
     1981 cacgcgagcc gcgttgttca ccgtggactc gaccttcagc cggttgcgta cgatgcccgg
     2041 gtcctggagc aggcgttcga ccttcgcggg cttgaagcga gcgaccttcg ccggatcgaa
     2101 cccggcgaac gcgatccggt acccgtccct cttgttcaag atcgtcgacc aggacaggcc
     2161 cgcctgggcg ccctcgaggc tgagcatctc gaacaggcgt gcgtcgtcgt ggaccggcac
     2221 gccccactcc tcgtcgtggt acgcgaggta gaccggctgg tcgagcggga cccactcgca
     2281 gcgcttcggc atcggc
//
LOCUS       DASUYD010000054         5257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_247088, whole genome shotgun sequence.
ACCESSION   DASUYD010000054 DASUYD010000000
VERSION     DASUYD010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247088"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /locus_tag="VF195_02880"
     CDS             complement(<1..1237)
                     /locus_tag="VF195_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6579801.1"
                     /translation="MMVERPQPVRDLDWDPKRARAFTDGVADIYEELLTRLRDLPVSR
                     GWGVQEVRDAVAIPVPDEPMPEDEILEYLRTMTFEYSGYLGHPRFYAYISGAGTVPGA
                     AADLLASGLNMNTGGWRLAPSGTEIELAFTRWFAQEIFGLPVGSGGALTSGGAMANFI
                     ALKVARDAKAGWDIRKDGVGAGPTLGLYVSTETHIVSIRGADMLGLGSGNVRKVAVDD
                     GYRIRVHELRAAIAADRAAGIHPIAVVANGGTVSTGVVDPLEEIADVCRDEDLWFHVD
                     AAYGGPAMLADDLRPLFAGIERADSVAFDPHKWLYTPQSGGCVVVRDMELMRRAFDVD
                     YVAYVVKDEEHTDWGIDLGRHSPNFSRGFWALRIWVSLLAHGRAAYGRRISHDAALAR
                     YLGARAEESDEFELMTPVGL"
     gene            complement(1234..1959)
                     /locus_tag="VF195_02885"
     CDS             complement(1234..1959)
                     /locus_tag="VF195_02885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX6579802.1"
                     /translation="MIDVADLGTILGVWAHPDDDIYLSAGLMAAAVKAGRRVVDVTAT
                     RGEGGSMDEERWPPESMGEVRTNELLRSLDVLGVTEHHFLEGAVDIDMDTGLDAAGAE
                     QVRRIMQEVDPDTVLTFGPEGMTGHVAHQDVSRWAGEAFQAVAKPGAHLFHAVFPNSH
                     AEEWLEKLAPFDIFRPGTPNVVADEEVDLDHDVEGRFLDQKIASIKEHASQIEALYEV
                     FGDEGFRRFMAKEAFVLAATKGA"
     gene            complement(1995..2243)
                     /locus_tag="VF195_02890"
     CDS             complement(1995..2243)
                     /locus_tag="VF195_02890"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6579803.1"
                     /translation="MGEKGDMGCDETLREVERYVDGECLRELTIQVEAHLAGCTDCND
                     HAEFKRHMKVLIATKCRGEVLPEGLAERIRERIRTASP"
     gene            complement(2236..2829)
                     /locus_tag="VF195_02895"
     CDS             complement(2236..2829)
                     /locus_tag="VF195_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579804.1"
                     /translation="MANASRVPAPADQDLLERFDRDVLPLLPGLYGAALRMTRNPADA
                     EDLLQETTLRAYRGFASFREGTNLKAWLYRILTNSFINIYRKKQREPKTVEGPDDIDE
                     WFLFDRLGAKSVERSAEEDVLEKIPDADVKAALESIPENFRMAVLLADVEGFSYREIA
                     EITDVPIGTVMSRLHRGRKALERALYGVAKERGLVDG"
     gene            2909..4906
                     /gene="ftsH"
                     /locus_tag="VF195_02900"
     CDS             2909..4906
                     /gene="ftsH"
                     /locus_tag="VF195_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011700234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX6579805.1"
                     /translation="MPEQDRKPFGRRPVPGSAQPRPRSALVVVLVVAGSLVLFNTFLT
                     QAASTPVPFSEFRRAIDNGNLVKSEPVTITTTTISGLVRTADGDETYVATIPPGYVPT
                     DLVDDLSARGYEVDGQQPNALTGFLLQMVLFIALFGGLYYFLFRRIGGGATSALNLGR
                     NKVKIYDRKEMKTSFTDVAGVDEAKEELREIVDFLKNPKKYQRLGGRIPKGVLLLGPP
                     GCGKTLLARAVAGEANVPFFFMSGSEFVEMFVGLGAARVRELFQQAKEKAPALVFLDE
                     IDTIGKGRAGPMSGSFGGHDEREQTLNQLLVEMDGFDASKGVIIMAATNRPDVLDPAL
                     IRPGRFDRQVVVDLPDLNGREEILRVHARGVALDPSVDLNTIAARTPGFTGADLENVV
                     NEAALLAARREKNAVSMEELEEAIDRATAGLERKSRVVSDSQKERVAVHEMGHALVAL
                     NCKEIDPLHRVTIIPRGAAALGITMTRPLEDRYVVTEPQLKDQMAFAMGGQAAEEIYF
                     DGVISTGAQNDLEKATDLARAMVSQFGMSDEVGPLSLGRPDPNGWMPGSVPKVSAHTS
                     ELIDKEVRRLLEEARGRAAKILNENRDLLNRLSALLIATEVIDGDELKAYVEGAKPIP
                     DPATIRPIPQPAAAEERAPAEPTPVARAVSMPPAPPMPATD"
     gene            complement(4903..>5257)
                     /locus_tag="VF195_02905"
     CDS             complement(4903..>5257)
                     /locus_tag="VF195_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579806.1"
                     /translation="DVDAIEGYLMALRRETLVEAGFFDEKFRWYRTADIEFSFRVKDL
                     DRRATIVDVPVTKHHHRMWFETRPEDRARWSKRNFYRFLDRFRDRWDLLVDPLPEGER
                     PQHHHDDHDHEESGG"
BASE COUNT          856 a         1829 c         1731 g          841 t
ORIGIN      
        1 agaggcccac cggcgtcatc agctcgaact cgtccgactc ctccgctcgg gcgccgaggt
       61 agcgagcgag cgcggcgtcg tgggagatcc ggcgcccgta ggccgcccga ccgtgcgcga
      121 ggagcgacac ccagatcctc agcgcccaga agccgcggct gaagttgggc gagtgccggc
      181 cgagatcgat gccccagtcc gtgtgctcct cgtccttgac cacgtaggcg acgtagtcga
      241 cgtcgaaggc gcgccgcatc agctccatgt cccggacgac cacgcagccc cccgattgcg
      301 gcgtgtacag ccacttgtgc gggtcgaagg ccaccgagtc ggcgcgttcg atgccggcga
      361 acagaggacg caggtcgtcg gcaagcatcg ccggtccgcc gtacgcggca tcgacgtgga
      421 accagagatc ctcgtcgcgg cacacatcgg cgatctcctc gagcggatcg acgacgcccg
      481 tggaaaccgt tccgccgttc gccaccaccg ctatcggatg gatgccggcc gcccggtccg
      541 ccgcgatcgc cgcgcgaagc tcgtgcacac ggatccggta gccgtcgtct accgcgacct
      601 tcctgacgtt gcccgacccg aggcccagca tgtccgcgcc gcggatcgac acgatgtgcg
      661 tctccgtcga gacgtacagg ccgagcgtgg gaccggcgcc gacgccgtcc ttccggatgt
      721 cccagcccgc cttcgcatcg cgcgccacct tcagcgcgat gaagttggcc atcgccccgc
      781 ccgaggtcag cgcgccaccc gagccgacgg gcagaccgaa gatctcctgc gcgaaccatc
      841 gggtgaaggc gagctcgatc tcggtccccg acggtgcgag gcgccatcca ccggtgttca
      901 tgttcaggcc cgacgcgagc aggtcggcgg cagcaccggg taccgtcccc gcgcccgaga
      961 tgtaggcgta gaagcgcgga tgtcccagat aaccggaata ctcgaacgtc atcgtccgca
     1021 ggtactccag gatctcgtcc tcgggcatcg gctcgtcggg aacggggatc gcgacggcgt
     1081 cgcggacctc ctggaccccc cagcctcgcg agaccgggag atcgcggagc cgggtgagca
     1141 gctcctcgta gatgtcggcg acaccgtcgg tgaacgcgcg cgctcgcttc ggatcccagt
     1201 cgagatcgcg gacgggttgg ggccgctcga ccatcatgct cccttcgtcg ccgccaggac
     1261 gaacgcctcc ttcgccatga agcggcggaa gccctcgtca ccgaagacct cgtacagagc
     1321 ctcgatctga ctggcgtgct ccttgatcga cgcgatcttc tggtcgagga accgtccctc
     1381 cacatcgtgg tcgagatcga cctcctcgtc cgcgacgacg ttgggggtgc cgggccggaa
     1441 gatgtcgaag ggcgcgagct tctcgagcca ctcctcagcg tgactattcg ggaagacggc
     1501 gtggaacagg tgggcgccgg gcttcgcgac cgcctggaac gcctcgcccg cccatcggga
     1561 cacgtcctgg tgcgccacgt ggccggtcat cccctcgggc ccgaacgtga ggaccgtgtc
     1621 gggatcgacc tcctgcatga tccgtcgcac ctgctctgca ccggccgcat cgagtccggt
     1681 gtccatgtcg atgtcgactg cgccctcgag gaagtgatgc tccgtcacgc cgaggacgtc
     1741 gaggctccgc aggagctcgt tcgtgcggac ctcgcccatc gactcgggcg gccatcgttc
     1801 ctcgtccatc gacccgccct cgccgcgtgt ggccgtgacg tcgacgaccc gtcggcccgc
     1861 cttcaccgcg gccgccatca ggccggcgga gagatagatg tcgtcgtccg gatgcgccca
     1921 gactccgagg atcgtcccga ggtcggccac gtcgatcatg cggagggatg ctagccgggc
     1981 accaggttgt cccgctaggg gctcgcggtg cggatccgct cgcggatccg ctccgccaat
     2041 ccctccggga gcacctcgcc gcgacacttc gtggcgatca ggaccttcat gtgcctcttg
     2101 aactcggcgt ggtcgttgca gtcggtgcag ccggcgaggt gcgcctcgac ctggatcgtg
     2161 agctcgcgga gacactcgcc gtcgacgtag cgctcgacct cgcgaagcgt ctcatcgcac
     2221 cccatgtcgc ccttctcacc catcgacgag tcctcgctcc ttggctacgc cgtacaacgc
     2281 gcgctccagg gctttccttc cccggtgcag gcgcgacatc accgttccga tcggcacgtc
     2341 cgtgatctcc gcgatctccc tgtaggagaa gccttccaca tctgccagga gcaccgccat
     2401 ccggaaattc tcggggatcg actcgagtgc cgccttcacg tcggcatcgg ggatcttctc
     2461 gagcacgtct tcctcggccg agcgctcgac gctcttggcg cccagtctgt cgaacaggaa
     2521 ccattcgtcg atgtcgtcgg gaccctcgac ggtcttcggc tcgcgctgct tcttccgata
     2581 gatattgatg aaggagttcg tgaggatgcg gtacagccat gccttgaggt tcgtgccttc
     2641 ccggaacgac gcgaaccccc gataggcccg gagggtcgtc tcctgcagca gatcctcagc
     2701 gtcggcgggg ttcctcgtca tccggagggc agccccgtag aggccgggga gcagcgggag
     2761 cacatcccgg tcgaaccgct ccaggaggtc ctggtcggcc ggtgcaggca ccctgctcgc
     2821 gttcgccatc cgacgaccag tatccctggt gcgccgccct gcggcttcgc cggtcgtcac
     2881 aactacaagg cggtgggaag attgggccat gcccgagcaa gaccgcaaac ccttcggacg
     2941 ccgccccgtt ccgggctcgg cacagccacg gccgcgatcg gcactggtcg tcgtgctcgt
     3001 ggtggccggc tccctggtgc tgttcaacac attcctcacg caggcggcat ccacccccgt
     3061 gccgttctcg gagttccgga gggcgatcga caacggcaac ctcgtgaaga gcgagccggt
     3121 cacgatcacg accaccacga tcagcggtct tgtacggacc gccgacggcg acgagaccta
     3181 cgtggcgacg atcccgcccg ggtacgtccc caccgatctc gtcgacgacc tcagcgcccg
     3241 gggctacgag gtcgacggtc agcagccgaa cgcgctgacc ggcttcctcc tccagatggt
     3301 cctgttcatc gcgctcttcg gcggcctgta ctacttcctc ttccgccgga tcggcggggg
     3361 cgcgacctcc gctctcaacc tcggacgcaa caaggtcaag atctacgacc gcaaagagat
     3421 gaagacgtcc ttcacggacg tcgcgggcgt cgacgaggcg aaggaggagc tccgagagat
     3481 cgtcgacttc ctgaagaacc cgaagaagta ccagcgactc ggcggccgga tccccaaggg
     3541 cgtcctgttg ctcggtccgc ccggatgcgg gaagacactg ctcgcccggg cggtcgccgg
     3601 ggaggcgaac gtccccttct tcttcatgtc ggggtcggag ttcgtcgaga tgttcgtggg
     3661 actgggcgcc gctcgtgtcc gcgagctgtt ccagcaggcg aaggagaagg ccccggccct
     3721 ggtgttcctc gacgagatcg acacgatcgg gaaggggcgc gccggcccga tgagcggcag
     3781 cttcggtggc cacgacgagc gcgagcagac cctcaaccag ctgctggtgg agatggacgg
     3841 tttcgatgcc tccaagggtg tgatcatcat ggcggccacc aaccggccgg acgtcctgga
     3901 cccggcgctg atccggccgg gccggttcga ccgccaggtg gtcgtggatc tccccgacct
     3961 gaacggacgc gaggagatcc tccgcgtgca cgcccgcggc gtagcgctgg atccctccgt
     4021 cgacctgaac acgatcgccg cacggacgcc cgggttcacc ggggcggacc tcgagaacgt
     4081 cgtgaacgag gcggcgctgc tcgcggcgcg ccgcgagaag aacgcggtct cgatggagga
     4141 gctcgaagag gcgatcgatc gggcgaccgc gggactcgag cggaagtcac gggtggtctc
     4201 cgactcgcag aaggagcggg tggccgttca cgagatgggc cacgcgctcg tcgcgctcaa
     4261 ctgcaaggag atcgatcccc tgcaccgcgt cacgatcatc ccccgcggcg cggcggcgct
     4321 cgggatcacg atgacgaggc cgctcgaaga tcgctacgtg gtgaccgagc cgcagctgaa
     4381 ggaccagatg gcgttcgcga tgggcggaca ggcggcggag gagatctact tcgacggcgt
     4441 gatctccacc ggcgcgcaga atgacctcga gaaggccacg gatctcgcgc gcgcgatggt
     4501 ctcccaattc gggatgagcg acgaggtggg gcccctttcc ctgggccgcc ccgatcccaa
     4561 cggctggatg cccggctcgg tgcccaaggt gagtgcccac acgtcggagc tgatcgacaa
     4621 ggaggtccgg cggctgctgg aggaggcacg cggccgcgcg gccaagatcc tgaatgagaa
     4681 ccgcgacctg ctgaacaggc tctcggccct gttgatcgcc accgaggtga tcgacgggga
     4741 cgagctgaag gcctacgtgg agggggcgaa gccgatcccg gatcctgcca cgatcaggcc
     4801 catcccccag ccggcggcgg ccgaggagcg ggccccggcc gagccgacac cggtggcacg
     4861 cgcggtctcg atgccacccg ccccgccgat gcccgcgacg gactagccgc cgctctcctc
     4921 gtggtcgtga tcgtcgtgat ggtgttgcgg ccgctcgccc tcggggagcg gatccacgag
     4981 cagatcccac cgatcgcgga accggtcgag gaaccggtag aagttcctct ttgaccaccg
     5041 agctcggtcc tccggcctcg tctcgaacca catccggtga tggtgcttcg tgacggggac
     5101 gtccacgatc gtggctcggc gatcgaggtc cttcacccgg aacgagaact cgatgtcggc
     5161 cgtgcggtac cagcggaact tctcgtcgaa gaaccccgct tcgacgagcg tctctcgacg
     5221 gagcgccatc agatagccct cgatcgcgtc gacgtct
//
LOCUS       DASUYD010000055        33629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_26584, whole genome shotgun sequence.
ACCESSION   DASUYD010000055 DASUYD010000000
VERSION     DASUYD010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 33629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33629
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26584"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="VF195_02910"
     CDS             <1..828
                     /locus_tag="VF195_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579807.1"
                     /translation="LAMTIEAGKEAEDRLRAGRLRSEKSIAKARRDIRTAEAARQRFI
                     MANLRLVVSVARKYQGQGLPLLDLIQEGNIGLMRAVELFDWRRGFKFSTYATWWIRQG
                     ITRAIADRSRSIRLPVHIHDRLRKLNRTRFQFAQSMGREPSREELAKALDTTVDEIDD
                     LTEMGRREPLSLQSPVGEDTELGDLIEEIDSEAGFAEVEDQLLREEIGGAVDKVLDER
                     ERHVVALRYGLENGQPLSLRETGKVLGLSGERVRLIEREALRKLRDSNLITGVALGG"
     gene            complement(895..1194)
                     /locus_tag="VF195_02915"
     CDS             complement(895..1194)
                     /locus_tag="VF195_02915"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6579808.1"
                     /translation="MKSPWAYPENRYYYEWYAEEPSHERSDMEIKQDLLDQIRSGPYA
                     DQYDIEVDVKKGVVILTGTASTTVAKRAAGDDSWDTRGVTDVSNQIQVRSTVPVS"
     gene            complement(1289..1525)
                     /locus_tag="VF195_02920"
     CDS             complement(1289..1525)
                     /locus_tag="VF195_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579809.1"
                     /translation="MTEEFPREERGRALGIVTAMNTLGGIAVGLFAFGPSVLSFDVSW
                     RAYAGPIFPSEGGPPVQDEHAGVWGIATVSASPS"
     gene            complement(1522..1908)
                     /locus_tag="VF195_02925"
     CDS             complement(1522..1908)
                     /locus_tag="VF195_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6579810.1"
                     /translation="MIPPERNAFARRPLPAQLLRLLSSATFFEGYDTLVLSFVLSFVL
                     VDLGGSEAQAGAIQAIVGLGAAVGFLLSAQADRIGRRRLLLNTIVGCSACAALTALSP
                     NLAFLTGPQFHSQIFLASEWAVAITS"
     gene            complement(1895..2593)
                     /locus_tag="VF195_02930"
     CDS             complement(1895..2593)
                     /locus_tag="VF195_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX6579811.1"
                     /translation="MTSELGAERAVRGDLDAWWRFGAPIADLIVRALFRVRVAGIHHV
                     PLEGPAILAFVHVSVLDGPCLAAEVAWRRRRTVRFLVASEIFDAPVSGWFLRRYRQIP
                     VRRGKSDAGALDEVIATIRRGAVAAIAPEGTVNPTPGSLQRIRSGIARIALPTGAPVI
                     PVGIWGTQHRWSRAGRRWGGPFRPRLGFAFGPAIEPSGDTSVQGDIDAVVTRVREALE
                     RQLAEARSLVGDPA"
     gene            complement(2590..3699)
                     /locus_tag="VF195_02935"
     CDS             complement(2590..3699)
                     /locus_tag="VF195_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6579812.1"
                     /translation="MTREAIGAVVDAIGSEVRIEDLAIATPETGEVLVRLEASGVCHS
                     DLWAIDHGNWGAPFPMLLGHEGAGVVEEVGEGVDTPHVGDPVVLAWAVPCGTCGPCRR
                     GVPRRCSHAWQQPPRVRRARDAAPLAGTLSLGTLASHTVVHAAQAIPMPPELPLRSAC
                     LLGCGVSTGVGAAIQTAKVWPGARVAVIGLGGIGLAALQGARIAGAERLIAVDVAAPK
                     LAWAERFGATDTIDASATDAVAAVRELTQGHGVDFAFEATGVPVVVSQAVAMLDYAGV
                     AVAIGVPPIPSEVTLSWNGTEDGAYPRKTSLLITDGGDPIPSEDFPEMARWALEGQLD
                     LGGMVTRELGLGDLAEAFRAMLAGEVIRTVVVFDP"
     gene            3747..4898
                     /locus_tag="VF195_02940"
     CDS             3747..4898
                     /locus_tag="VF195_02940"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6579813.1"
                     /translation="MDVQTLRDRAQARYPEFLDVLETMVNVDCGSYSPEGVNQIADLC
                     ERRFLDHGWEVDRRSHDGEPTLGDLVIGTLRGAGGPNVLLVGHMDTVFDDGTVAKRPF
                     SIEGDIARGPGVSDMKAGLLAGFFAIHVLQDVGLDAFGTITYVCNPDEEIGSPFSGPV
                     IRELAPAHDVALVLEGARANGDIVSSRKGITDFTITIEGKAAHSGVEPEKGRNAILQA
                     AHTILALQGLNDRWPGVTSNVGVVRGGTRSNVVAERCELHVDLRSPELAALEEAEAEV
                     HRICADPAVPDVRIRIDAHGWHRPMEKGEGGRRLVDIAVGAAAELGFELRDAATGGAS
                     DANTTSSAGTPTIDGLGPIGGDDHGPAEWVDLTSIVPRTALLAAIISRV"
     gene            4904..6001
                     /locus_tag="VF195_02945"
     CDS             4904..6001
                     /locus_tag="VF195_02945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6579814.1"
                     /translation="MSGWPPQELHPDAYRHLVEGAPAILYIDRPDELSTNLYTSPQIV
                     DLLGYSVREWMEDAELWVRSIHPDDRERVVQEHRDSNAHAERFMAEYRILTKAGREVW
                     IRDEAVPVRSEDGTVVYWRGVMVDITPQKLAEGQLRSSLEEVRRMVAQRRELAQLLET
                     AQEEERRRIAADIHDDPIQVMSAVDMRLQLLLARPDRFDAHELGELAEELRSAIDRLR
                     NLLFELRPVALDLEGLTPAIAMYVEHSAKEAGWSWEVINELDDEPLPDARVSFYRIAQ
                     EGITNARKHADATHLDVRLTSVESGLLLRIVDDGDGFDPSEKAAPGHLGLATVTERAE
                     LAGGWCRVLSAPGEGTVLECWMPQIAPGPTS"
     gene            6016..6150
                     /locus_tag="VF195_02950"
     CDS             6016..6150
                     /locus_tag="VF195_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579815.1"
                     /translation="MAGREGLARGVDGPILFLHVRAMRRGTVGTVATDAMDQEIDLAD
                     "
     gene            complement(6126..6533)
                     /locus_tag="VF195_02955"
     CDS             complement(6126..6533)
                     /locus_tag="VF195_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX6579816.1"
                     /translation="MSRLAAAWWAWRNRREVGRWLGFAWRVVPPSATDRTDLVAEARL
                     RTAFAREPRTRGAPSLTVRVHARTAFLGGHLPSDVHDLAYAIARHTTGVLRVECAVRD
                     RGRRRTPETHLHPGGVAGLPPPPPEHQSARSIS"
     gene            6715..7065
                     /locus_tag="VF195_02960"
     CDS             6715..7065
                     /locus_tag="VF195_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6579817.1"
                     /translation="MIVIEMWTYRTDVAVQPAVDLVGFGIEARDGSIGKVDESTADAG
                     KSYLVVDTGPWILGRKVLLPAQTVERIDLETRTVFVDRTKEEIKNAPEFDPERGIDPT
                     YQDELGGYYGRYYS"
     gene            complement(7120..7866)
                     /locus_tag="VF195_02965"
     CDS             complement(7120..7866)
                     /locus_tag="VF195_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6579818.1"
                     /translation="MRELQTPDERGDDAAPATPAPDHWSGSKHVVQVRRHVWNWLAPR
                     IGGGAVLEIGPGLRPTAPVATSTFIDASTHALRCLAARGATTAAVEATLPFPDDSFDA
                     VLAFEVIEHVDDDTGLLGEIARVSRPGGVLILSTPVHTTMWSPLDESCGHVRRDEPPT
                     LFEKVRTAGFEIGGYSWTPAPSRLITSIRARALRSNRQLSTAFVQALVFPIHAAIQRL
                     FAKVRWQAPAEPVPEGADDVMLWATKVHRG"
     gene            8020..9426
                     /locus_tag="VF195_02970"
     CDS             8020..9426
                     /locus_tag="VF195_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579819.1"
                     /translation="MRTDAEGPRGPVRHDLLALTVVATLVGACTPSPEATPSTVTPPA
                     TSPSANAPATIPQPSVHGDLPLAPEGERVDTAMPTFSDPTNITNPLFPVSRQASAVLL
                     GHVDDQPFRTEVTLLPETRIIGWQGRLIEVAVSQYNAFLGGRIAEVAYDLYAQADDGS
                     VWYFGEDVFDFRDGAIVVTEGTWLAGKDGPAAMIMPADLQVGDVYRPENAPGIVFEEV
                     TVASVREALDGPLGPIQGGMLASELHMDGGTEDKLFAPGYGEFSTSGGGDVEALALAV
                     PTDTAIGSMPRELTAMSEGALSAFAAAGSGSWKVATTAASDVVSAWKTYRSEDVPSLI
                     EPLLETAVDTLDAAVDARSPARARNAAIDAARLSFDLQLRYRPVTEIDLARMDLWAAQ
                     LLVDEAAGHFEGVAADAFAMDYVRDRIRGALDGADLARVNTALGEIQVAVVDEEPAAA
                     AEAAGRLRDILAGLEPAG"
     gene            complement(9521..9910)
                     /locus_tag="VF195_02975"
     CDS             complement(9521..9910)
                     /locus_tag="VF195_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013864439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6579820.1"
                     /translation="MDKVVHFEIPVDDASRAREFYASAFEWELYEADMGGGNMYTTVT
                     TTPIDEATRLPTEPGAINGGLMQRSSDTPAPVITIGVTSIDESLKKVDAGGGSIVQPR
                     TEIPELGAYAYFKDTEGNVVGLWETRI"
     gene            complement(10259..10882)
                     /locus_tag="VF195_02980"
     CDS             complement(10259..10882)
                     /locus_tag="VF195_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEX6579821.1"
                     /translation="MTTATITRNREHLYKASPRKPEIVRVPEIGFVMIDGHGDPNTST
                     EYADAIQALYSLSYTLKFALKKELGLKSRVAPLEGLWWADDMAEFSPERKGDWSWTMM
                     IAQPEEATPERFEAARAQIARKKDLPALSRARLERFEEGPCAQILYVGPYSDEGPTIA
                     NLHAFIRDGGYSFEGSRQKHHEIYLGDPRRSSPEKWRTIIRQPLAEE"
     gene            complement(11145..11238)
                     /locus_tag="VF195_02985"
     tRNA            complement(11145..11238)
                     /locus_tag="VF195_02985"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11201..11203),aa:Sec,seq:tca)
     gene            11292..11705
                     /locus_tag="VF195_02990"
     CDS             11292..11705
                     /locus_tag="VF195_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6579822.1"
                     /translation="MTEPASPKQRHIALILAKDLAANLASAMLLVDPDGDLVFFNEPA
                     ERILGRPYAEAQMSRAELAKAFKPVDESGAPIPLADLPLASAFREGIPSHGHLRIESM
                     DGRTHDLEVVAVPLFAQEDQLVGGLAVFWEPAGEA"
     gene            11707..12546
                     /locus_tag="VF195_02995"
     CDS             11707..12546
                     /locus_tag="VF195_02995"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6579823.1"
                     /translation="MRARFWGSRGSLASPGPDTVRTGGNTSCVEVRPDDGSLVILDSG
                     TGIRALGATLDGTAPRRIDILLSHLHLDHLEGLGFFAPLWDRTAELHIWGPPSPTRSL
                     SDRISQYLSPPLFPVRLAEVPAHLELHDAPYEPWMLGSATIIARPILHRGPTVGYRIE
                     DGGRSLAYLTDHEPALAVDLSKVEPEWVSGFEVAAGVDVLIHDCQYTEEEYPERAGFG
                     HSSTHHVADFARLASARRLLLFHHDPNHSDDELEALCERVRELWRDGAVDLAREGEEV
                     VFG"
     gene            12602..13414
                     /locus_tag="VF195_03000"
     CDS             12602..13414
                     /locus_tag="VF195_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019873951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEX6579824.1"
                     /translation="MEAAFFDLDKTVISRSSTLALSRPLYRAGMVSRGQLLRGAYAQL
                     VYLLVGADEGKMERLKEGMLQLTKGWDRAQVERLVEDVILEVIDPYVYAEALDLMELH
                     RSEGRRIYIVSSSPEEIVRPLARHFGVSGVIATRAAIGADGRYTGQLEYYAYGEEKAA
                     AVRAVAHRVGIDLTRSFAYSDSVTDLPLLGAVGHPVAVNPDKELRRVAEEQGWPIRDF
                     HRPVRMRNRLASAVPGPRASVTAAFAAAGVAAVLIWVVLRSRAAGRRAASSS"
     gene            13411..14481
                     /locus_tag="VF195_03005"
     CDS             13411..14481
                     /locus_tag="VF195_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX6579825.1"
                     /translation="MRVVPLVLGFVVLLGDACDGSPSPASPLDPSTIEPSSSSEACAA
                     VEVNGATAQRFEVDGSCVPGDVLVLYRCSPTAVPVLRISSVNGPTLFLGGPFAVRVSS
                     LPAQVRFAGAAGGTEVLIADPLRSPTSTPAGSSSLSASPSAEGDPLVEPERFVYVREE
                     GRTERWLRLEGRRALHDPPIVWIIGDSIMDGGRKEVEAALADWSLTLDAEVGRSSSSG
                     VELAQSAVEQDADAVVIELGTNDTSPAVFRAHLVETLDAVAGVPLVIWQTARGPEDDL
                     SIPEVNAAIREVAPTYPNVSIADWTAFVPDEAVQTDGIHPDEGFQDLEAELLVPFLSE
                     WRDALDRRGATSCGRDIVRESS"
     gene            complement(14687..14941)
                     /locus_tag="VF195_03010"
     CDS             complement(14687..14941)
                     /locus_tag="VF195_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579826.1"
                     /translation="MGEIVSLAEYRDRQNPGLVMMRRLEGAVGRLDVVVRRRPGRMAP
                     TIERELLLIARAVSSGMPRDAADRAERLADLLEHPAASGS"
     gene            complement(15001..16071)
                     /locus_tag="VF195_03015"
     CDS             complement(15001..16071)
                     /locus_tag="VF195_03015"
                     /inference="COORDINATES: protein motif:HMM:NF014110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEX6579827.1"
                     /translation="MTELLVGTKKGLFVLRGDAGAEKPFDVAARAFPGNVVEFAIRDP
                     RSGRYFASVTSGHFGPRVMHTMDPTGEWEPTTGLGYPEGSDWSLERIWSIEPGEADGL
                     MYAGVAPAGLFTSTDGGMTWELNRALYDQPSRSSWQPGAGGLCLHSIATWPNDPSRLA
                     IGISAVGVWLSEDGGASWRHGNQGLVPRYVPEEAREGTIDLCVHNMHRSPLRPERLFM
                     QFHGGVYRSDDAGESWISIAEGLPADFGFPMAIDPADPDSAFVIPMDSDGDRVTVDGR
                     VRVYGTRDAGESWTSLSDGLPQEDAFLTILRQAFAVDGEGAGMGLYFGATSGDVFGSA
                     DAGASWFTAHERLAPVNSVRVA"
     gene            complement(16068..16334)
                     /locus_tag="VF195_03020"
     CDS             complement(16068..16334)
                     /locus_tag="VF195_03020"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX6579828.1"
                     /translation="MAIVRLRAPLKDLAGGSREVAIEGASVGEVLRELERQHPRIEGW
                     VLDEHGRVRRHVNVFMNGERVREDAAVASDATVHVLPSISGGRR"
     gene            complement(16345..16827)
                     /locus_tag="VF195_03025"
     CDS             complement(16345..16827)
                     /locus_tag="VF195_03025"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6579829.1"
                     /translation="MTTDEPEFEPVRKTIRVDRSAEEAFRLFTEHIDRWWPVEVLSRA
                     ADEQYGDGVKVERVVFEPRAGGRLYEVTSEGIEGVWAEVVAFEPPGRIMLAWKPNDRP
                     EPPTEVEIHFDPDGAGTIVRLEHRGWDKLGARAAEAREGHDGGWQVPLERFVAAAAAG
                     "
     gene            complement(16824..16946)
                     /locus_tag="VF195_03030"
     CDS             complement(16824..16946)
                     /locus_tag="VF195_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579830.1"
                     /translation="MIGSVHPEVSRELQRARREEIVRALKGCRRTFGRSRKEQR"
     gene            complement(16943..17293)
                     /locus_tag="VF195_03035"
     CDS             complement(16943..17293)
                     /locus_tag="VF195_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012919892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6579831.1"
                     /translation="MTRDPAGAVFAALADPTRRRVVHELSKDGPLTATELARRIPVSR
                     QAIAKHFDALEEADLATATRVGRETRYELRTQAFAEAEAWMRAIGAMWDQRLAAFKEF
                     VESVPSGSTGRRNR"
     gene            complement(17290..17718)
                     /locus_tag="VF195_03040"
     CDS             complement(17290..17718)
                     /locus_tag="VF195_03040"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6579832.1"
                     /translation="MEIPDEVRRELEIHAPRERVWAALTEPDQLLRWFPTKRAEIDLR
                     PGGGAILEWDEAVAEAVVDEVDPPGRLVFRWRPEGLGRPFTTVSFTLEESTHGTSTHV
                     SLVESGFASLPDQIETQSQKGNDEGWAHELQELKEYVEAA"
     gene            complement(17816..19021)
                     /locus_tag="VF195_03045"
     CDS             complement(17816..19021)
                     /locus_tag="VF195_03045"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6579833.1"
                     /translation="MPSHQVALRRRSVAFAVALLAVVAGFFLWPSQDAAPVRSDLDEE
                     TSSPYSGPDNYLAWVPGGFDDPAFRQKMERLPGLDEAVVVAGDTLWLRRTEDAQGLVV
                     DEPTSPFAFPIDAFAVDANDYAPFVGPSVRDEIVRALGTGRAVLGEHSASLRRLGPGG
                     RLVFRTGSVRVGAVVPDDAVGWAEVLLNREVGRRLGIAHERYLLAQPSEALARPVWKR
                     KLLPFVGEDPLRVDVAGATRYVRVASGVRPPILVKQRFGEFAATPQADPADLTIDPAW
                     VERNIVSTEVPLLGTVTCHRKLIPMVRGALYEIAASSLASEIEVYSGCWASRTVSRSP
                     TAPPSYHAYGAAIDINAPQNPYGTKPTMNREIVRIFESWGFNWGGDFLIPDGHHFEFW
                     MVPAQLRAS"
     gene            complement(19042..21525)
                     /locus_tag="VF195_03050"
     CDS             complement(19042..21525)
                     /locus_tag="VF195_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013861353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6579834.1"
                     /translation="MTERMLDDTLTATSFAPEAADGPLPSRARVVIVGGGIAGSSIAY
                     HLAGLGITDVLLLERARIASGTSWHAAGLVARVRGSHPMTELANYGVDLYRGLADETG
                     VDVHFRPTGSLTLAENEGRMTELRYAAAIARHHGTEARLLEPSEVPEVWPLISTDGLV
                     GALLQPGDGTVNPGYAALALAKGAHDRGATIREGIRVEELLLVGRAMTGVRTDRGDVE
                     AETVVLACGLWTRDLAATVGVPVPLYAAEHVHVSTGPIDGAHDLLPLVRGLDGSFYAR
                     HHAGGLMIGAFEPNGRPRSTSSIPEDWAFGEFGPDWEHFAPTRANGSRRIPALEDAEF
                     TRFLRAPESFTPDVNFCLGETAEVRNLFVAAGFNSQGIIYSPGAGRALAEWIREGAPT
                     FDASEVDVRRFATSQANERYLHERTREALGRLYAMHWPNLQASTARGIRRSPLYDRLE
                     TAGACFGELNQWERPMWFAPPGVQPEYGYSFGRQNWFPHVAEEHRAARDAVALFDLSS
                     FAKIEVTGADALSLLQRVCTNDVDVGVGRLVYTLFLNLSGGIENDGTVTRLAADRFLV
                     ITPTATQHRTFEWLRAEAGGRAVSIDDVTTAFATIALMGPRSRELLGSLTAADLSNGG
                     FPFFSAQEIQIASAPALAIRASFVGELGWELYVPSEFAVHVYDAVVEAGQGFDLRHAG
                     YHALDSLRIEKGFVHVGHDVGPTDDPFSAGLGHVVKLGKDFIGAEAARRAKATPGTHR
                     LVSLTLEDPGPVLLHGESVIADGKIVGTVMSAAYTHTIGAAVGLAMVEASVVADRETP
                     LRVDIAGEMVKATLSVRPLYDPSGARMRA"
     gene            21599..25270
                     /locus_tag="VF195_03055"
     CDS             21599..25270
                     /locus_tag="VF195_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6579835.1"
                     /translation="MSEAGTAMREERRFVTALFADLVGSTALGERLEPEELKLVVGDA
                     IGRIVGAVEAFGGTIKDLAGDGVLALFGAPVAHEDDPERALLAGLRIVEEMGEFADEV
                     CRSWGIDGFGVRVGVESGPVVVGAVGAGSRVEYGAMGDAVNVAARLQTQADPGTILVG
                     EGTAARVEPLFEWGPPVQLELKGKSGTVAARSVIAPLATSVSTRGIEGVQAPVVGRER
                     ELAVIRELARAALEGTGAIAVMTGEPGIGKTRLLSEFREMFVEGSAPQGRPLWLEGKC
                     VSYGESMTYWPFRDLVRSWLGVLADEPEMRVRVALRRNVERLFGDRASELTPYLAAML
                     GLTLEPDDQARLGELSPEALQYRTFEVVRHWLQRLAEEGPVAVALEDLHWADATSLQL
                     LERLLPDTEDSALLLVLTLRQERDHPAWRLKDDAARELPHRTTEMALEALSGDAGLDL
                     LRSLVGAGTLPEDMEGRILEHADGNPFFLEELVRSLADAGSLVRDGDGWRFDHEANVE
                     IPPTIEKVILARIDRLDPQPHAALMAAAVLGREFGLPLLEAVTGDGHVKDSLSSLMRL
                     DLLREARRWPEPEYRFKHALIQEAAYRTLVVDERRALHAKAATWLEDRYAGREEEVAG
                     LLAHHWLAAADEDKAVAYLTMAGDRARQEYALDEAIASYRELLPILGERGEQDAIALV
                     LFKLALAFHMSLRFAEANEMYQRAFDLWRWPDPAAEPPSATLRVGTSFLPNDPDPRSA
                     IAWPNIQLCMQLFDRLVEAWPERTIVPSLAERWEISDDGLRYVFHLREDLTWSDGEPL
                     TAHDVEYGIKRVLNPDAPGSSVAIYFVLEHGQDHYLRTTEDPDLIGVRALDDRTLEFR
                     LAAPAPYFMSVMNRPDGGPQPRHAIERDGDAWTETGKQVVSGAFEIVERETDRLVLRR
                     REETGSPRPGNVETVEYLRREIPEAIAAYARDELDAVTVRYTPKTADLVPGVSDDAHL
                     GAASWSGFLAFRHADPRMANVELRRALATAIDRGTLAEHVPSNLVVANGGIVPPALQG
                     HTPDIVPRFDPEAAREHLRRSGLSRDELQGLEIAGIETWLDDFLLVVSGTWKEVLDLD
                     VPVRPWTLEQALSIGDPTEMAPIVITGWLPGYADPEYFLRLLFQTDSKTNYGGFSDPE
                     FDQLIERARQERSDRSRLELFHEADRMAVADRIACIPLVYGRSMSFVKPWVTGWWEFG
                     KSSSSVADLMTASATSSAR"
     gene            complement(25291..25971)
                     /locus_tag="VF195_03060"
     CDS             complement(25291..25971)
                     /locus_tag="VF195_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579836.1"
                     /translation="MHKGERLRFVAIVGVVIAVLSAGFALAGDAFDDPADEPASTCEP
                     TDEVVDDGDTIEDPEGTEGEEPVEGEEPVEGEEPVEGCDDEATDDGESDETEGTDDTE
                     SGDEVVLERAEACEAAATPDDEEGGEVEETNDTEPVKLTGLENAIAHVLANCLKNENA
                     PGLVNALERLVANMERKESHDEWKAEREAEREAAKAEREAALDAAKAEREAAREAAKA
                     ARELNHSS"
     gene            complement(26097..26528)
                     /locus_tag="VF195_03065"
     CDS             complement(26097..26528)
                     /locus_tag="VF195_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018067853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6579837.1"
                     /translation="MSVPARINIVTLGVDSVARSKVFYEALGWELAGSVGDEICWFRT
                     SDSYLGLFGREDLAKDAGLRSEPTAEFGGITLAINVGSDAEADAAFEAAVAAGARILK
                     PAEHTDWGGYSGYFADPDGHPWEIAYNPSFPIGEDGRITIS"
     gene            26602..27039
                     /locus_tag="VF195_03070"
     CDS             26602..27039
                     /locus_tag="VF195_03070"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HEX6579838.1"
                     /translation="MPAVRLNPMLRVMWKLHRFALRVSGGRVGSRMGSMMVLLLETTG
                     HRSGRPRTVGLSHLEMDGRYFVMASYAGEDRDPAWAKNLRAHPRATVTLRGRSTPVVA
                     RALEGAEHDALFDRFVEADASYREYRERTTREIPVFELRPEGA"
     gene            27045..27959
                     /locus_tag="VF195_03075"
     CDS             27045..27959
                     /locus_tag="VF195_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX6579839.1"
                     /translation="MPSITDIEGLTVGHWTDEVALTGCTVVLPPPGTIASCEVRGGAP
                     GTRGTDILQPGTIIEVAHAIVLTGGSAFGLASAGGVERYFEERGIGSEIGPVRVPTVP
                     AAVIFDLGVGDPARRPGADEGYAACIAASTDVAEGRVGVGTGATVAKLWGPERALPGG
                     VGTWAVRDGELVVGALVVVNAVGEIVDEDGSILLGPRLEPGERREDLVEQLGSSAAAN
                     TTLGVVATNAVLSKEDVRRLATVGNDAFDVAIRPAHTIYDGDTVFALATQQVDASFEQ
                     VAASVEAAVTTSIRRSVIRGDGSGAAGA"
     gene            complement(27922..28833)
                     /locus_tag="VF195_03080"
     CDS             complement(27922..28833)
                     /locus_tag="VF195_03080"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEX6579840.1"
                     /translation="MDDGLHTRSFRLLFQAPFAASTLLSFLGARAVPGIEETRDGTYV
                     RSVRHPSGAAVVAMSPRSDNVAFRASSEGSLDLDDLRDRARRAFDLDADAAAIDAALA
                     SDPMMMPLVARIPGIRVPGTFDGFELVVRAIFGQQVSVAGARTSLGRLVSAVGTPLER
                     PSGAITHLFPTAEGMAELPPEAFGMPRSRAETIRRVAALVAGGELDLSGDAPPGETLG
                     RLGEVPGVGPWTLAYVAMRALRDPDAFMAGDLGVRKGFEALGLESTQTAMLARAERWR
                     PWRAYAVMHLWHHHVSRRQPLLNHRRG"
     gene            28912..30009
                     /locus_tag="VF195_03085"
     CDS             28912..30009
                     /locus_tag="VF195_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579841.1"
                     /translation="MDQVARVILALEAPEVAEEVMHVLDRSGRARVVATAGDDRQLAE
                     AVRQLEPDVVVAEPSLLSGARACGVVLAVDVRESVSSLRTAIRAGAAGYFVWPKERDD
                     LLVAAAAAVRRVQQAATRGAVVAVHGGRGGAGVTFVATHLAAAFARRGSAILIDADPV
                     FGDVAQALGAPTGEEAEPAHTFADAAALGEDLGPEQLRSALWRHESEVDVLLPPPPEE
                     AIRLGPEDLRLVTDVAAGAADAVVVHLPRALGAMTRAGAETSDSLVEVVTLDVVSFRA
                     TSRALEACAPLHLEGRVSFVVNKAARSEITPGDVHRVFGEPARAVLPHDRAVRSAQER
                     NRLLPPKGRMAHRFDRLADALSTPVVEEMAS"
     gene            30046..30735
                     /locus_tag="VF195_03090"
     CDS             30046..30735
                     /locus_tag="VF195_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579842.1"
                     /translation="MDERVLLVEDDPSIREMAGLGLGNAGFRVTTCADGREGLERLRR
                     DPFDLAVLDVMLPSLDGFEITREVRKDSRIPIVMLTARADATDVIVGLELGADDYVTK
                     PFDMPVLVARCRAVLRRAAAEPADPALRVGDLEIDVGGFAARKGGRDLALTATEFRLL
                     VELARRPGQVLTRDVLLARVWDYEYLGDSRLVDVAVQRLRGKIEDDPAHPRTISTVRG
                     VGYRLERVGAE"
     gene            30732..31961
                     /locus_tag="VF195_03095"
     CDS             30732..31961
                     /locus_tag="VF195_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6579843.1"
                     /translation="MTGGRGSPGRLVLVVVLVAAVVSAALATAVFVLVRDARFHDSLE
                     RARTEAVFDLTIAGPLLQGSGEPGAVVETFREERGIHAILVEDGRTIDADPASGISIP
                     PELRAIAARGQLGYDRIEAGGEPLLLVGGPTPDRGAELYFAFSETGIHEDLRELMTAL
                     AIGWIAIVVVAFALARVAASRIASLAEAEAWGRRFTSDVSHELRTPVAALVSEASVLR
                     EHLTAMPPDARRAAELLVGDVARLRRLVEDLTNLASLDAGREQVRTEPFDLATLVTGT
                     VRSRGWAGEVQIRSDPVPVTSDRTRVDRIVANLIGNALDHGGGAVVVHVRGSGDGASV
                     EVADTGPGIAPEHLEHVFDRFYRADPSRSGPGSGLGLAIARENARLLGGDVEARSEVG
                     VGSRFTLRLPEDPVAEP"
     gene            32031..32912
                     /locus_tag="VF195_03100"
     CDS             32031..32912
                     /locus_tag="VF195_03100"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX6579844.1"
                     /translation="MKRLIAMMAVLATLATACAESGAQPLGPAPSADGPSPSPGSPSP
                     TLSPTSPEPPTPAQSMTYELWFNYDGSLFVTHRTEPFTPSVGTRALEALLEGPSAPER
                     AANLGTTINAGTRLNGLTIDDGVATVDLDATFVGEETPAIAVGSLAQIVFTLTQFDRI
                     GGVVFEVDGGPLTNFGGYEIDGAQRRTNFADQLPAILVQSPGIGERVSSAVLISGTAN
                     VFEAVVSIAILDQNGKVIASTFTMATCGTGCRGTWETDVAYEVAATQPGTVRVYEVSA
                     MDGSKINVVNIPVTLTV"
     gene            33246..>33629
                     /locus_tag="VF195_03105"
     CDS             33246..>33629
                     /locus_tag="VF195_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEX6579845.1"
                     /translation="MSADPLAKVRRQLRAQLRERVDARGLSAAPRTERRLRVREEALS
                     LMRESGALLPQRDLARVVNEVSDEVVGFGPIEFLLRDPGVTEVMVNGPDDVYVEREGR
                     IERVEDRLFEGEESVLHLIERIVGPL"
BASE COUNT         4987 a        11613 c        11805 g         5224 t
ORIGIN      
        1 ctcgccatga cgatcgaggc ggggaaggag gccgaggacc gtctccgtgc aggccgtctc
       61 cggtccgaga agagcatcgc gaaggcccgc cgtgacatcc ggacggccga ggccgcgcgt
      121 cagcgtttca tcatggcgaa ccttcgattg gtcgtctccg tggcccggaa gtaccagggc
      181 cagggtctcc ctctcctcga tctgatccag gagggcaaca tcggcctcat gcgagccgtg
      241 gagctgttcg actggcgccg aggcttcaag ttctcgacct acgccacgtg gtggatccgc
      301 cagggcatca cccgggctat cgcagaccgc tcccgctcga tccgcctgcc cgtccacatc
      361 cacgaccggc tccggaagct gaaccgcacc cggttccagt tcgcgcagtc gatgggccgc
      421 gagccctccc gcgaggagct ggcgaaggcc ctcgatacca ccgtcgacga gatcgacgac
      481 ctgaccgaga tgggccggcg cgagccgctc tccctccagt caccggtcgg tgaggacacc
      541 gagctgggcg acctgatcga ggagatcgat tccgaggcag ggttcgcgga ggtcgaggac
      601 cagctgctcc gcgaagagat cggcggggcc gtcgacaagg tactggacga gcgcgagcgt
      661 catgtcgtgg cgctccggta tgggctcgag aacggacagc ctctgtcgct ccgggagacc
      721 gggaaggtgc tcgggctgtc gggtgagcgg gtgcgcctga tcgagcgcga ggcgctcagg
      781 aagctccgcg actcgaacct gatcaccggc gtggccctcg gcggctagtc cgtccgatcg
      841 gtccgtatcg ccgatcggcc gccctcctgg gcgtccgccc acggtccgtc cgcgctagga
      901 gaccggaacc gtgctccgga cttggatctg gttgctcacg tcggtcacgc cgcgggtgtc
      961 ccaggagtcg tcgccggcgg cgcgcttggc cacggtggtg ctcgcggtgc cggtcaggat
     1021 cacgacgccc ttcttgacgt cgacctcgat gtcgtactga tcggcgtagg gaccgctccg
     1081 gatctggtcg agcagatcct gtttgatctc catgtccgag cgctcgtggc tcggctcttc
     1141 ggcgtaccac tcgtagtagt agcgattctc gggatatgcc caggggctct tcatcggaga
     1201 ccacctccag cgcgttccta ccccctctgc gcccgaccta atcaggctct tcggacacga
     1261 acgagccgca tcaggccctc tccgacgatc aagagggcga cgccgacacc gtggcgatgc
     1321 cccagacgcc ggcgtgctcg tcctggacgg gtggcccccc ttcggacggg aagatgggac
     1381 cggcgtaggc acgccaggac acgtcgaagg agagcacgct gggtccgaac gcgaagagcc
     1441 cgaccgcgat cccgccgagc gtgttcatcg cggtcacgat gccgagggca cgacctcgct
     1501 cctcccgcgg gaactcttcg gtcacgatgt gatcgcgacg gcccactcgg acgccaggaa
     1561 gatctgcgag tggaactgcg ggccggtgag gaacgcgagg ttcggtgaga gcgccgtgag
     1621 cgcggcgcac gccgaacagc cgacgatcgt gttcaacagg aggcgccgcc tgccgatccg
     1681 gtcggcctgc gcggacagga ggaagccgac ggcggcaccg aggcccacga tcgcctggat
     1741 cgccccggct tgcgcctccg agccgccgag gtcgacgagc acgaacgaca gcacgaacga
     1801 cagcaccaag gtgtcgtagc cctcgaagaa ggtcgccgac gacaggagtc ggaggagctg
     1861 cgccggtagc ggtcgtcgcg cgaaggcgtt ccgctcaggc gggatcaccc accagcgagc
     1921 gcgcctcggc caactggcgc tcgagcgcct cgcggacgcg cgtcacgacg gcgtcgatgt
     1981 cgccctggac ggacgtgtcg ccgctcggtt cgatcgccgg cccgaacgcg aagccgagcc
     2041 gaggtcggaa ggggcctccc cagcgccggc cagccctcga ccatcgatgc tgcgttcccc
     2101 agatccccac cgggatcacg ggcgcgccgg ttgggagcgc gattcgcgcg atcccgctcc
     2161 ggatccgctg gagtgaaccc ggcgtggggt tcacggttcc ttccggcgcg atcgccgcga
     2221 cggcgccgcg gcggatcgtg gcgatcacct cgtcgagcgc gccggcgtcg ctcttgccgc
     2281 gacgcacggg gatctggcgg taccgacgga ggaaccagcc gctaacgggg gcgtcgaaga
     2341 tctcggacgc gaccaggaag cgcacggtcc gtcgccggcg ccatgcgacc tcggccgcga
     2401 ggcacggccc gtcgaggacg ctgacgtgca cgaacgcgag gatcgcgggc ccctccagcg
     2461 ggacgtgatg gatacccgca acgcgcaccc ggaacagagc acgtacgatc agatccgcga
     2521 tcggcgctcc gaaccgccac caggcatcga ggtcgcctcg gacggctcgt tctgctccga
     2581 gctccgaggt cacgggtcga acaccacaac cgtgcggatc acctctccgg ccagcatcgc
     2641 ccggaacgcc tcggcgagat cgccaagccc cagctcacgc gtcaccatcc cgccgaggtc
     2701 cagctgcccc tcgagtgccc agcgcgccat ctccgggaag tcctccgacg ggatcggatc
     2761 cccgccgtcc gtgatgagga gcgacgtctt ccgagggtag gcgccgtcct cggtcccgtt
     2821 ccacgagagc gtgacctcgg aggggatcgg cggcacgccg atcgcgacgg cgacccccgc
     2881 gtagtccagc atcgccacgg cctgcgacac cacgacgggg accccggtcg cttcgaacgc
     2941 gaagtcgacg ccgtgcccct gcgtgagctc gcgcaccgcg gcgacggcgt cggtggcgga
     3001 ggcgtcgatc gtgtccgtcg caccgaaccg ctccgcccag gcgagcttcg gggcggcaac
     3061 gtccacggcg atcaaccgct cggcgcccgc gatcctcgcg ccctgcagcg ccgcgagacc
     3121 gataccaccc agtccgatca ccgccacgcg ggcgccgggc cacaccttcg ccgtctggat
     3181 cgccgcgccg acccccgtgg agacgccgca gccgaggagg cacgcgctcc tcagagggag
     3241 ctcgggcggc atcgggatcg cttgcgccgc gtggaccacg gtgtgcgagg cgagcgtgcc
     3301 gagcgagagg gtgcccgcga gcggcgcggc gtcccgcgca cggcggacgc ggggaggctg
     3361 ctgccacgcg tgcgagcagc gacgagggac gccgcgccgg cacggtccgc acgtgccgca
     3421 cgggaccgcc catgccagca cgacggggtc gcccacatga ggcgtatcca cgccctcgcc
     3481 cacctcctcg acgacgccgg ccccctcgtg gccgaggagc atcgggaacg gtgcccccca
     3541 gttcccgtgg tcgatcgccc acaggtccga gtgacacacg ccgctcgcct cgagacgcac
     3601 gaggacctcg ccggtttccg gtgtggcgat cgcgaggtcc tcgatccgga cctcgctgcc
     3661 gatcgcgtcc accaccgcgc cgatcgcctc tcgcgtcacc gtctccctct cgctgcgagc
     3721 ggctcatgct agcgtcggcg tcgcccatgg atgtccagac gctccgcgat cgtgcccagg
     3781 ccagataccc ggagttcctc gacgtgctcg aaacgatggt caacgtcgat tgcggctcgt
     3841 actcgccgga gggcgtcaac cagatcgcgg acctctgcga gcgccggttc ctcgatcacg
     3901 ggtgggaggt cgatcgtcgg tcgcacgatg gagaaccgac gctcggcgat ctcgtgatcg
     3961 gcacgctccg aggtgcgggg ggaccgaacg tgctgctcgt ggggcacatg gacacggtct
     4021 tcgacgacgg gacggtggcc aagcgtccgt tctcgatcga gggggacatc gcgcgcggcc
     4081 ccggtgtgtc ggacatgaag gcgggcttgc tggcgggctt cttcgccatc cacgtgctgc
     4141 aagacgtcgg tctcgacgcc ttcggcacga tcacctacgt gtgcaatccc gatgaggaga
     4201 tcgggtcgcc cttctcgggc ccggtcatcc gcgagctggc cccggcgcac gatgtcgcgc
     4261 tggtacttga gggagctcgc gcgaacggcg acatcgtgag ctcgcgtaag gggatcacgg
     4321 acttcacgat cacgatcgaa ggcaaggcgg cgcactccgg cgtcgagccc gagaagggcc
     4381 ggaacgcgat cctccaagcc gcacacacca tcctggcgct ccaggggctc aacgaccgct
     4441 ggccgggggt gacgtccaac gtcggtgtgg tgcggggcgg gacccgttcg aacgtcgtcg
     4501 ccgagcgatg cgagctccac gtcgacctcc ggtcgcccga gctcgctgcg cttgaggagg
     4561 cggaggcgga ggtgcaccgg atctgtgccg acccggcggt ccccgacgtg cgtatcagga
     4621 tcgacgccca cggctggcat aggccgatgg agaagggcga agggggccga cgcctcgtgg
     4681 acatcgccgt cggggccgca gcggagctcg gcttcgagct ccgcgatgcg gccacgggcg
     4741 gcgcctccga tgcgaacacg acgagctcgg ccggtacgcc gacgatcgac ggcctcggcc
     4801 ctatcggcgg ggacgatcac gggccggccg agtgggtgga cctcacgagc atcgtgccgc
     4861 gcaccgctct gctcgcggcg atcatctcga gggtgtgagc cggatgtcgg ggtggccgcc
     4921 gcaggagctg cacccggacg cctaccggca cctggtggag ggggcgccgg cgatcctcta
     4981 catcgaccgt cccgacgagc tctcgacgaa cctgtacacg agcccgcaga tcgtggacct
     5041 cctcgggtac agcgtccgag aatggatgga ggacgccgaa ctctgggtcc ggtcgatcca
     5101 tcccgatgac cgcgagcgcg ttgtgcagga gcaccgggac agcaacgccc atgccgagcg
     5161 gttcatggcc gagtaccgga tcctcacgaa ggcgggtcgg gaggtgtgga tccgcgacga
     5221 ggccgtgccg gtgagatcgg aggacggcac cgtcgtgtac tggcgcggcg tgatggtgga
     5281 catcacgccc cagaagctcg cggagggcca gctgcgttcg agcctcgagg aggtccgacg
     5341 catggtcgct cagcggcgcg agctggcgca gctgctggag acggctcagg aggaggagcg
     5401 ccgcaggatc gcggccgaca tccacgacga cccgatccag gtgatgagcg cggtggacat
     5461 gcggctgcag ctgctgctcg ctcgtcccga tcggttcgat gcacacgaac ttggcgagct
     5521 ggcggaggag ctgcgatcgg cgatcgaccg cctgcggaac ctcctcttcg agctccgtcc
     5581 cgtggcgctc gacctcgagg ggctgacgcc ggcgatcgcg atgtacgtcg agcactccgc
     5641 gaaggaggcc gggtggtcct gggaggtgat caacgagctc gacgacgagc cgctccccga
     5701 cgcccgcgtg tccttctatc ggatcgcaca ggaagggatc accaacgccc ggaagcacgc
     5761 cgacgccacc catctggacg tccgattgac ctcggtggag agcggactcc tgctgcggat
     5821 cgtggacgac ggtgacgggt tcgatccatc ggagaaggcg gcgccgggtc acctcggcct
     5881 cgcgaccgtg accgagcgcg ccgagctcgc cggagggtgg tgtcgcgtgc tcagcgcacc
     5941 cggtgaaggg acggtcctcg aatgctggat gccgcagatc gcgcccggcc cgacgtcctg
     6001 acgtccgatg gaggggtggc gggccgggag ggtctggctc gcggagtgga cgggccgatc
     6061 ctgttcctcc acgtccgtgc gatgcggcgc ggcaccgtcg gcacggtcgc gaccgacgcg
     6121 atggatcagg agatcgatct cgccgactga tgctccggag ggggaggagg gagcccggca
     6181 acgccgccgg gatggagatg cgtctccggt gtgcgtcggc gaccacggtc gcggaccgcg
     6241 cactcgacgc ggagcacgcc ggtcgtgtga cgagcgatcg cgtacgcgag gtcgtggacg
     6301 tccgacggga ggtgcccgcc gaggaaggcg gtccgtgcat gcacccgaac ggtcagcgag
     6361 ggggcgcctc gggtgcgagg ctctctcgcg aacgcggtgc gaagcctcgc ttcggcgacg
     6421 aggtcggtcc ggtcggtggc cgacggcggc acgacccgcc acgcgaatcc gagccaccgt
     6481 cccacctccc tccgattccg ccacgcccac catgcggcgg ccaggcggct cacggggaag
     6541 agtttccaca accacgagat cacgcagaac acgtcctttc cggttcctct gggacatctc
     6601 tacccgcgat gcccgcgcac cgaacccgcc gaaaacgttt ctcctcggtg cgtcccgggt
     6661 agcggcgatc tgagcgaccg tggaccgacg gccgctcatc ccccaaggag gtggatgatc
     6721 gtgatcgaga tgtggacgta tcgcaccgat gtggcggtgc agccagctgt ggatctcgtc
     6781 ggtttcggca tcgaggcccg cgacggaagc atcgggaagg tcgacgagtc gaccgccgat
     6841 gccgggaaga gttacctcgt cgtggatacc ggtccctgga tcctcggccg gaaggtgctg
     6901 ctcccggcgc agaccgtaga gcggatcgac ctcgaaacac ggaccgtctt cgtcgaccgg
     6961 acgaaggagg agatcaagaa cgctcccgag ttcgacccgg agcgtgggat cgaccccacg
     7021 tatcaggacg agctcggcgg gtactacggt cggtactact cgtaaccggc ggggagctcg
     7081 acggacggac ccggagggcg atcgccctcc gggtccccgt catccgcggt ggaccttcgt
     7141 cgcccagagc atcacgtcat ccgcgccctc tggcacgggc tcggccggag cctgccatct
     7201 caccttcgcg aacagccgct ggatggcggc atggatcggg aacacgagcg cctggacgaa
     7261 cgcggtcgac agctggcggt tcgatcgaag tgcccgcgcc cggatgctcg tgatgagtcg
     7321 agagggagcg ggggtccacg aatagccacc gatctcgaag ccggccgtcc gcaccttctc
     7381 gaacagggtc ggtggttcgt ccctacggac gtgaccgcac gattcgtcca gcggggacca
     7441 catcgtggtg tggaccgggg tggagaggat cagcaccccg ccgggccggc tcacgcgggc
     7501 tatctcgccg aggaggcccg tgtcgtcgtc gacgtgttcg atgacctcga aggcgagcac
     7561 cgcgtcgaac gagtcgtcgg ggaaagggag tgtcgcctcg acggccgccg tcgtcgcgcc
     7621 gcgtgccgcg agacaccgca atgcgtgtgt cgaagcgtcg atgaacgtcg aggtggccac
     7681 gggcgcggtc ggccggagcc cggggccgat ctccagcacg gcgccgccac cgatccgcgg
     7741 cgcgagccag ttccacacgt ggcgccggac ctgaacgacg tgcttcgatc cgctccaatg
     7801 atcgggtgcg ggcgtcgcgg gggccgcatc atcaccccgc tcgtccggtg tctggagttc
     7861 tcgcacggca gctccggtcc ggtcggagcg gggagcatac ccggcgtcct cccggccggg
     7921 cttcttcttg gccgccgcac gggaaccctc gtggccgttc agcgaccgtt catcctcggt
     7981 cgggcacgct gctcgcctgg gtacgcagag gaggaacgga tgcgcacgga tgcggaggga
     8041 ccgaggggtc cggtccgaca cgatctgttg gccctcaccg tcgtcgcgac actggtgggt
     8101 gcgtgcacgc caagcccgga ggccacgccc agcacggtga cgccgccggc gacgtctcct
     8161 tcggccaacg cgcccgcgac gatcccgcag ccgtcggtcc acggggatct ccccctcgcg
     8221 cccgagggcg agcgggtcga caccgcgatg cccacgttct ccgatcccac gaacatcacg
     8281 aatccgctgt tccccgtctc gaggcaggct tcggcggtgc tcctcgggca cgtcgatgac
     8341 caaccgttcc ggaccgaggt cacactcctg cccgagacgc ggatcatcgg atggcagggt
     8401 cgactgatcg aggtggccgt ttcgcaatac aacgccttcc tcggtggaag gatcgccgag
     8461 gtcgcgtacg acctgtacgc gcaggccgac gacgggtccg tctggtactt cggcgaggac
     8521 gtgttcgatt tccgggacgg tgcgatcgtc gtcaccgagg ggacctggct cgccggcaag
     8581 gatgggccgg cggcgatgat catgccggcg gatttacagg tgggtgacgt ctaccgaccc
     8641 gagaacgcac ccgggatcgt gttcgaagag gtcaccgtgg cttccgtgcg ggaggctctg
     8701 gatggcccgc tcggtccgat ccagggcggg atgctcgcgt cggaactgca catggacggg
     8761 gggacggagg acaagctctt cgcccccggg tacggggagt tctccacgtc aggcggaggc
     8821 gatgtcgagg cgctggccct cgccgtaccg acggacacgg cgatcgggtc gatgccgcgc
     8881 gagctcacgg cgatgtcgga gggcgctctg tccgcgttcg ctgcggccgg atcgggcagc
     8941 tggaaggtcg cgacgaccgc ggcctcggac gtggtgtccg cttggaagac ctaccggtcg
     9001 gaggacgtgc ccagcctgat cgaacccctg ttggaaacag cggtcgacac gctcgacgcg
     9061 gccgtcgatg ctcgcagccc cgctcgggct cggaacgccg cgatcgatgc agcccggttg
     9121 agcttcgacc tccagctgcg gtaccggccc gtgacggaga tcgatctcgc tcggatggat
     9181 ctctgggcgg cgcagctcct ggtggacgag gccgcggggc acttcgaagg ggtcgcggcg
     9241 gacgctttcg cgatggacta cgtccgtgac cggatccggg gagccctcga tggggccgat
     9301 ctcgctcgcg tgaacacggc gctcggcgag atccaggtcg ccgtggtcga cgaggagcct
     9361 gctgcggcgg ccgaggctgc cggtcggctc cgggacatcc tcgcagggct cgaacccgcg
     9421 ggctgacgcg cgtaaccgtc gccgaacccg ctcgttgcgt cgttccgaca gttctggccg
     9481 cgcgccgcgg cgtccggtcg accggttcag gaggtggcga tcagatgcgg gtctcccaca
     9541 acccgaccac gttgccctcg gtgtccttga agtatgcgta ggcgcccaac tccgggatct
     9601 cggtgcgtgg ctggacgatg ctgccgccgc ccgcgtccac cttcttcagt gactcgtcga
     9661 tggaggtcac accgatcgtg atcaccgggg cgggcgtgtc cgagctccgc tgcatcaggc
     9721 cgccgttgat ggcgccgggc tccgtgggga gccgtgttgc ctcgtcgatc ggagtggtgg
     9781 tgacggtcgt gtacatgttg ccgccaccca tgtccgcctc gtacagctcc cactcgaagg
     9841 cggaggcgta gaactcccga gcccgtgacg catcgtcgac cgggatctcg aagtgcacga
     9901 ccttgtccat gattcctcct cccgggcctc cagggccccg catgttcctc ctagcctatc
     9961 cggctgccag gtctcgccag gagcccgcac acgtcgtgcc ttcgagcctg cgaacatgca
    10021 ggtcagctga ggcggacgga actctgcaag aacccgaccg gggcgggtcg gagcttgtgg
    10081 gtcagcgctc gtgaccggag gcgtgtggcg tggatttcac ctcgccaccc acccgggtag
    10141 aggagaggcg ccgggtggtc aagagcgcat cgacttcagg cgcgggcgcg cgaggtgcga
    10201 tcgaccttca ggtcacgggc ccccgcccgg aacgccggcc agcccccgcg tccgtggctc
    10261 actcctccgc gagcggctga cgaatgatcg tcctccactt ctcgggggaa ctgcgccgtg
    10321 gatcgccgag atagatctcg tggtgcttct ggcggcttcc ttcgaacgag taccccccgt
    10381 ctcggatgaa ggcgtgaagg ttggcgatgg tcgggccctc atcgctgtac gggccgacgt
    10441 agaggatctg ggcgcacggc ccctcctcga agcgctcgag gcgagccctg gagagcgccg
    10501 ggaggtcctt cttgcgggcg atctgggccc gtgcagcctc gaaccgctcc ggggtcgcct
    10561 cctcgggctg ggcgatcatc atcgtccagc tccagtcgcc cttgcgctcc gggctgaact
    10621 ccgccatgtc gtcggcccac cacaggccct cgagcggggc gacgcgactc ttcagaccca
    10681 actccttctt cagggcgaac ttcagtgtgt aggacagcga gtagagggct tggatggcgt
    10741 ctgcgtattc ggtggaggtg ttggggtctc catgcccatc gatcatcacg aaccctatct
    10801 cgggaacgcg aacgatctcc ggcttgcgtg gggatgcctt gtacaggtgc tctcggtttc
    10861 gcgtgatcgt cgcggtcgtc atgtcctgcc ccttccctgg gccacgagag agccgtctgg
    10921 catcgcccgc atgtcaggat gtcggtccag gcctcccggg cgtagggcct tctgggccgg
    10981 cgattccggg acccgcggtc aagacgtgtt cgctcaggaa gagatagacg atcgtcgggc
    11041 caaacatcag ctcgatccat gaggcggtgg atcgtcgaac ccgataggtt cggggaggga
    11101 aagtggagtc acgcggactc ccgatccctc cccgatctcg taccgcggag gcggacggga
    11161 atcgaacccg ccagagagct cgtcgccctc tcgtcggttt tgaagaccgc ggggcccacc
    11221 aggaacccag acgcctccgt gcccgaggat agcggcgccg gcctcggggc tcgtggtgta
    11281 gcgtcgcgga cgtgacggag ccggcgtcgc cgaaacagcg acacatcgcg ctgatcctgg
    11341 cgaaggacct ggcggcgaac ctcgcgagcg ccatgctcct cgtcgacccg gacggggatc
    11401 tcgtcttctt caacgaaccg gcggagcgga tcttgggacg tccgtacgcc gaggcccaga
    11461 tgagtcgcgc cgagctcgcg aaggccttca agcccgtcga cgagtcggga gcgccgatcc
    11521 cgctcgcgga cctgccgctc gcctcggcgt tccgcgaagg gatccccagc cacgggcatc
    11581 tgcggatcga gtcgatggac gggcggaccc acgatctcga ggtcgtggcc gtgccgctgt
    11641 tcgcccaaga ggatcagctc gtgggcggac tcgccgtctt ctgggagccg gccggggagg
    11701 cctgacgtgc gcgccaggtt ctggggctcc cggggctcat tggcgtcgcc gggaccggac
    11761 accgtccgga ccggcggcaa cacctcgtgc gtggaggtgc gccccgacga cgggagcctg
    11821 gtcatcctcg actcgggcac cgggatccgg gctctcggag cgaccctgga cgggacggca
    11881 ccgagacgca tcgacatcct gctgagccat ctccacctcg atcatctcga agggctcggg
    11941 ttcttcgcgc cgctgtggga tcggaccgcg gagctgcaca tctgggggcc gccctcgccg
    12001 acgcgatcgc tctccgaccg gatctcgcag tacctctcgc cgcccctgtt ccccgtacgg
    12061 ctcgccgagg tgcccgccca cctggaactc cacgacgctc cgtacgagcc gtggatgctc
    12121 ggctcggcga cgatcatcgc ccgtccgatc ctccaccgag gcccgaccgt gggttaccgg
    12181 atcgaggacg gaggccggag cctcgcctat ctcaccgacc acgagccggc gctggctgtc
    12241 gacctgtcga aggtggagcc cgagtgggtg tccgggttcg aggtcgccgc gggagtcgat
    12301 gtcctgatcc acgactgcca gtacacggag gaggaatacc cggagcgcgc cgggttcggt
    12361 cactccagca cccatcacgt cgccgatttc gcccgactcg cgtccgcgcg gcgactcctc
    12421 ctgttccatc acgatccgaa tcactccgac gacgagctcg aagcgctctg cgagcgggtt
    12481 cgcgagctct ggagggacgg cgcggttgac ctcgcccgcg aaggcgagga ggtcgtcttc
    12541 ggctgaggcg tccggcggtt ccccctcaac gtcacggggt ccccgtacga tggttcttcc
    12601 gatggaagcg gcattcttcg atctggacaa gaccgttatc tcacggtcca gcacgctcgc
    12661 actgtctcgc cccctgtatc gggcggggat ggtctcccgt ggtcagctgc ttcgcggggc
    12721 gtacgctcag ctcgtctacc tgctcgtcgg agccgacgag gggaagatgg agcgcctgaa
    12781 ggaagggatg ctccagctca cgaagggctg ggatcgcgcg caggtggagc ggctcgtcga
    12841 agacgtgatc ctggaggtga tcgatcctta cgtctacgcc gaggcgctcg acctgatgga
    12901 actccatcga tccgagggca gacgcatcta catcgtgtcg tcctcaccgg aagagatcgt
    12961 ccgaccgctg gcgcgccact tcggcgtttc cggggtgatc gccacgcgcg ccgcgatcgg
    13021 cgcggacggt cggtacacgg gccagctcga gtactacgcg tacggcgagg agaaggccgc
    13081 ggccgtccgc gccgtggccc accgcgtggg gatcgatctg acgcgttcct tcgcctacag
    13141 cgactccgtg accgacctgc cgctcctcgg cgccgtcggt cacccggtgg ccgtgaaccc
    13201 cgacaaggag ctgcgcaggg tggcggagga acagggctgg ccgatccgcg acttccatcg
    13261 gcccgtgcgg atgcggaacc gactggcttc ggccgtcccc ggtcccaggg cgagcgtgac
    13321 ggccgccttc gcggcggccg gggtcgccgc ggtgctcatc tgggtggtgc tgcggtcgcg
    13381 cgccgccggc cgccgcgccg cgtcgtcctc gtgagggtcg tcccgctcgt gctcggcttc
    13441 gtcgtcttgc tcggcgacgc gtgcgacggg agcccttcgc cggcgtcgcc gctcgacccg
    13501 tccacgatcg agccgagctc cagcagcgag gcttgcgcgg ccgtcgaggt gaacggagcg
    13561 acggcgcagc ggttcgaagt ggatggctcg tgcgtacccg gtgacgtgct cgtcctgtac
    13621 cgttgctccc cgacagcggt ccccgtcctc cggatctcct cggtgaacgg gcccaccctg
    13681 ttcctcgggg gtccgttcgc cgtccgggtg agctcgctcc cggcgcaggt ccggttcgcc
    13741 ggcgccgcgg gaggcacgga ggtcctgatc gccgacccgc ttcggtcgcc gacgtccact
    13801 ccggccggtt cgtcgagcct ctcagcgagc ccatcggcgg agggcgatcc cctcgtcgag
    13861 ccggagcgat tcgtctacgt ccgcgaggag ggccgcacgg agcggtggct ccggctcgaa
    13921 gggcggcggg cgctgcacga tccaccgatc gtgtggatca tcggggactc gatcatggat
    13981 ggcgggcgta aggaggtcga ggccgccctc gccgactgga gtctcaccct cgacgccgaa
    14041 gtgggtcgat cgtcgtcgtc cggcgtcgag ctcgcgcagt cggccgtcga gcaggacgcg
    14101 gacgccgtgg tgatcgaact cggcaccaac gacacgtctc ccgcggtgtt ccgcgcgcac
    14161 ctcgtcgaga ccctcgacgc cgtggcgggc gtcccgctcg tgatctggca gacggccagg
    14221 ggcccggagg acgacctgag catccccgag gtcaacgcgg cgatccgcga ggtcgcgccg
    14281 acatacccga acgtctcgat cgccgactgg accgccttcg taccggacga ggcggtgcag
    14341 acagatggga tccaccccga cgagggtttc caggatctcg aggccgagct cctcgtgccg
    14401 ttcctgtcgg agtggagaga cgcgctggat cggaggggag cgaccagctg tggccgggac
    14461 atcgtgcgcg agtcgtcctg acggcgctcg gagacgggag agcgggagga gcaggtatca
    14521 ggcgtctcgt ctgcatcgtg ctcgggctgc cggacctcac gtggcccggg gacctgagca
    14581 tgcctcccgc ggcaagactc gcgcgtgggt tctgctcccc ccgcagctac ctctcgatag
    14641 cgctccgacc gtacacccgc ctcgcgatgg tccgcaacgg cggaggtcag gagccggacg
    14701 cggccggatg ctccaggagg tccgcgagac gctcggcacg atccgccgcg tcgcggggca
    14761 tgcccgacga gaccgcccgc gcgatcagca ggagctcgcg ctcgatcgtg ggcgccatcc
    14821 tcccgggtcg ccgccggacc accacatcca accggcccac cgctccctcc aggcgccgca
    14881 tcatgacgag cccggggttc tggcggtcgc gatactccgc gagcgagacg atctctccca
    14941 cgggcccatc atgaaccccc cctctgacac gccggggagg ttcgcggatc ttgggctcga
    15001 ctaggcgact cggacggagt tcaccggcgc gagccgttcg tgcgccgtga accacgaggc
    15061 tcccgcgtcg gcggacccga acacgtcgcc cgatgttgcc ccgaagtaca ggcccatgcc
    15121 cgccccctct ccgtcgaccg cgaacgcctg tcggaggatc gtgaggaaag cgtcctcctg
    15181 ggggaggccg tcgctcagcg atgtccagga ctcgcccgca tcgcgcgtgc cgtagacacg
    15241 tactcgcccg tcgaccgtca cgcgatcgcc gtcgctgtcc atggggatca cgaatgcgga
    15301 gtcggggtcg gcgggatcga tcgccatcgg gaagccgaag tccgccggca atccctcggc
    15361 gatcgagatc cagctctcgc cggcgtcgtc gctgcggtag acgcctccgt ggaactgcat
    15421 gaacaaccgc tccggtcgga gcggcgaacg gtgcatgttg tgcacgcaga ggtcgatcgt
    15481 tccttccctc gcctcctccg ggacgtaccg ggggaccaga ccctggttgc cgtggcgcca
    15541 ggacgcgccg ccgtcctcgc tcagccacac gccgaccgcg gagatcccga tcgcgagccg
    15601 cgaggggtcg ttgggccacg tggcgatcga gtggaggcag agcccgccgg cgcccggctg
    15661 ccacgacgag cggctgggct ggtcgtacag cgcccggttc agctcccagg tcatgccacc
    15721 gtcggtggag gtgaacaacc cggccggcgc cacgcccgcg tacatcagcc cgtcggcctc
    15781 acccggctcg atcgaccaga tccgttccag gctccagtca ctcccctccg ggtacccgag
    15841 gccggtcgtg ggctcccact cgcccgtcgg gtccatcgtg tgcatcaccc gagggccgaa
    15901 gtgcccggac gtgacggacg cgaagtagcg accgctccga ggatcgcgga tcgcgaactc
    15961 cacgacgttg ccggggaacg cgcgggccgc gacgtcgaac ggcttctccg cgccggcgtc
    16021 gccccggagg acgaacagcc ccttcttcgt accgaccagc agctccgtca tcgcctgcct
    16081 cccgagatcg atgggaggac atggacggtc gcgtccgagg cgaccgccgc gtcctcccgg
    16141 acacgttcac cgttcatgaa gacgttcacg tgccggcgga cccggccgtg ttcgtcgagg
    16201 acccagccct cgatccgggg gtgttgccgc tcgagctccc ggagaacctc gccgaccgag
    16261 gcgccctcga tcgcgacctc acggctgcca ccggcgaggt ctttgagcgg cgcgcgcagc
    16321 ctgacgatcg ccatcttctc gagcctaacc ggcggccgcg gcggcgacga agcgctccaa
    16381 cgggacctgc catccgccgt cgtgaccctc ccgagcctcc gctgcccgag cgccgagctt
    16441 gtcccaccct cgatgctcca gcctcacgat cgtcccggcg ccgtccgggt cgaagtggat
    16501 ctcgacctcg gtcgggggct cgggccggtc attcggcttc cacgcgagca tgatccggcc
    16561 cggtggctcg aaggcgacga cctcggccca gacgccttcg ataccttcgg acgtgacctc
    16621 gtagaggcgg ccaccggcgc gaggttcgaa gacgacccgc tccaccttga cgccgtcgcc
    16681 gtattgctcg tcggcggccc gagagaggac ctcgaccggc caccatcggt cgatgtgctc
    16741 cgtgaagagt cggaacgcct cttcagccga tcggtcgacg cggatcgtct tgcggaccgg
    16801 ttcgaactct ggttcgtcgg tcgtcatcgc tgctccttcc gggatcggcc gaacgtacgg
    16861 cggcatccct tcagtgctcg gacgatctct tcccggcggg cgcgttgcag ttccctgctc
    16921 acctccggat gcacggaacc gatcatcgat tcctccttcc cgtcgagccc gagggcaccg
    16981 actccacgaa ctccttgaac gcggcgaggc gctggtccca catagccccg atggcacgca
    17041 tccaggcctc cgcctcggcg aaggcctggg tgcgcagctc gtagcgggtc tcgcggccga
    17101 cacgtgtggc ggtggcgaga tccgcttcct ccagcgcgtc gaagtgcttc gcgatggcct
    17161 ggcgtgagac cgggatccga cgagcgagct cggtggccgt gagcgggccg tccttcgaca
    17221 gctcgtgcac gacgcgtcgc cgcgtcgggt cggcgagcgc cgcgaacacc gctccggccg
    17281 gatctcgggt catgcggcct ccacgtactc cttgagctcc tggagctcat gtgcccagcc
    17341 ttcgtcattt cccttctgtg attgggtctc gatctgatcg gggagcgacg cgaacccgga
    17401 ctcgacgagg gacacgtgcg tggacgtgcc gtgcgtggac tcctcgagcg tgaacgagac
    17461 ggtcgtgaac gggcgtccga gtccttcggg ccgccaccgg aagaccagac gccccggcgg
    17521 gtcgacctcg tcgaccaccg cttcggcgac ggcctcgtcc cattcgagga tggcaccccc
    17581 ccctggccgc agatcgatct ccgcgcgctt cgtcgggaac caccggagga gctgatcagg
    17641 ctccgtgagg gccgcccaaa cgcgctcccg tggtgcgtgg atctccagct ctctcctgac
    17701 ctcgtccggg atctccatgc gtctcccctt cctcctgcaa cttgacggtt gcgtttatct
    17761 tatgcaacca tcaggttgca tgtcaagtcg ggtcacgacg cggcgggccg cggagctagg
    17821 acgctcggag ttgagccggg accatccaga actcgaagtg gtggccgtcg gggatcagga
    17881 agtcgccgcc ccagttgaag ccccagctct cgaagatccg gacgatctcg cggttcatcg
    17941 tcggcttcgt tccgtacggg ttctggggcg cgttgatgtc gatcgcggcg ccgtacgcgt
    18001 ggtacgaggg aggcgccgtg gggctcctcg agaccgtgcg ggaagcccag catccggagt
    18061 agacctcgat ctccgacgcg aggctcgatg cagcgatctc gtagagcgcg ccgcggacca
    18121 tcgggatcag cttccggtgg cacgtgaccg tcccgagcag cggcacctcg gtcgagacga
    18181 tgttccgttc gacccaggcg gggtcgatgg tcaggtccgc ggggtctgcc tggggcgtgg
    18241 cggcgaactc gccgaagcgc tgcttcacga ggatcggggg tcgcacaccg ctcgcgaccc
    18301 gcacgtacct cgtcgcgccc gcgacgtcga cccggagggg gtcctcgccc acgaagggga
    18361 ggagtttgcg tttccagacc ggacgcgcga gcgcctcgga cggctgcgcc aagaggtacc
    18421 gctcgtgtgc gatccccagc cggcggccga cctcgcggtt caggagcacc tccgcccatc
    18481 cgaccgcgtc gtcggggacg accgcgccga cccggacgga gccggtccgg aacacgagtc
    18541 tgccgccggg gccgaggcgg cgcaggctcg cgctgtgctc gcccagcacc gcccggccgg
    18601 taccgagcgc ccggacgatc tcgtcccgga cgctcgggcc gacgaacggt gcgtagtcgt
    18661 tcgcgtccac cgcgaaggca tcgatcggga acgcgaacgg gctcgtcggc tcgtccacca
    18721 ccagtccctg ggcatcctcg gtcctccgca gccacagcgt gtcgccggcg accaccaccg
    18781 cctcgtccag gccgggcaga cgttccatct tctggcggaa ggcgggatcg tcgaagccgc
    18841 ccgggaccca cgcgaggtag ttgtcgggac ccgagtaggg cgagctcgtt tcctcgtcga
    18901 ggtccgagcg aaccggtgca gcatcctggg acggccagag gaagaacccg gcgacgacgg
    18961 cgaggagcgc gacggcgaac gcgacggagc gccgccggag ggcgacctgg tgtgagggca
    19021 cgtcttccag gctactgctg gtcaggcacg catccgagca ccgcttgggt cgtaaagggg
    19081 acgcacggag agcgtcgcct tcaccatctc gcccgcgatg tccacccgta gcggcgtctc
    19141 ccggtccgcg acgaccgagg cctccaccat cgcgagcccg acggccgcgc cgatcgtgtg
    19201 ggtgtaggcc gcggacatca ccgtgcccac gatcttcccg tcggcgatca ccgactcgcc
    19261 gtggaggagg accggcccgg ggtcctcgag cgtgaggctc acgagtcgat gcgttcccgg
    19321 cgtcgccttc gcgcggcgag cggcctcggc accgatgaag tccttcccca gcttcacgac
    19381 gtgtcccagt ccggcggaga acgggtcgtc ggtcggaccg acgtcgtggc cgacgtggac
    19441 gaaccccttc tcgatccgga gcgagtcgag cgcatggtag ccggcgtgtc ggaggtcgaa
    19501 cccctgtccg gcctcgacga ccgcgtcgta cacgtgcacg gcgaactccg acgggacgta
    19561 cagctcccag ccgagctcac cgacgaacga ggcccggatc gcgagggcgg gcgcgctggc
    19621 gatctggatc tcctgcgccg agaagaacgg gaagcccccg ttcgagaggt cggccgcggt
    19681 gaggcttccc agcagctcac ggctccgcgg tcccatcagg gcgatcgtgg cgaacgccgt
    19741 cgtcacgtcg tcgatcgaga cggccctacc tccggcctcg gcacggagcc actcgaacgt
    19801 ccggtgctgc gtcgcggtcg gcgtgatcac gaggaaccgg tcggcagcca ggcgcgtgac
    19861 ggtcccatcg ttctcgatcc caccagagag gttcaggaag agggtgtaca cgaggcgccc
    19921 gacaccaacg tcgacgtcgt tcgtgcacac tcgttggagc agggacaggg cgtccgcgcc
    19981 cgtcacctcg atcttcgcga acgagctcag gtcgaagagt gccacggcgt ctcgcgcggc
    20041 gcggtgctcc tcggccacgt gcgggaacca gttctgccgc ccgaaggagt atccgtactc
    20101 cggctgtacg ccgggcggcg cgaaccacat cgggcgctcc cactggttga gctcgccgaa
    20161 gcacgcaccc gcggtctcca ggcgatcgta gagcggggaa cgccggatgc cgcgcgccgt
    20221 gctcgcctgg aggttcggcc agtgcatcgc gtagagccgg ccgagcgcct ccctcgtccg
    20281 ctcgtggagg tagcgctcgt tcgcctgcga cgtcgcgaac cgccggacgt cgacctcgga
    20341 ggcgtcgaac gtgggagcgc cctcccggat ccactcggcg agggcgcgac cagcccccgg
    20401 cgagtagatg atcccctggg agttgaaccc cgcggccacg aagaggttcc gcacctcggc
    20461 cgtctcgccg aggcagaagt tcacgtccgg ggtgaaggac tccggtgcgc gaaggaagcg
    20521 cgtgaactcc gcgtcttcga gcgcagggat ccgccggctc ccattcgctc gggtcggggc
    20581 gaagtgctcc cagtccggac cgaactcgcc gaacgcccaa tcctcgggga tcgagctggt
    20641 ggagcgaggg cgtccgttcg gctcgaacgc accgatcatc aacccgccgg cgtgatgccg
    20701 cgcgtagaag gaaccgtcga gaccgcggac gagcggcagc agatcgtgcg cgccatcgat
    20761 cggtccggtc gagacgtgga cgtgctcggc cgcgtacagc ggcaccggga cgccgacggt
    20821 cgcggccagg tcgcgggtcc acaatccgca tgcgaggacc accgtctcgg cttcgacgtc
    20881 gccgcgatcg gtccgcacac ctgtcatcgc ccggcccacg aggagcagtt cctcgacccg
    20941 gatgccctcc cggatcgtcg caccacggtc atgcgcaccc ttggcgaggg cgagcgcagc
    21001 gtagccgggg ttcacggtcc cgtcgccggg ctggaggaga gcgccgacga gaccgtcggt
    21061 cgagatcagc ggccacacct cgggcacctc ggagggttcc aggagacggg cctcggtccc
    21121 gtggtgacgg gcgatcgcgg ccgcgtatcg cagctccgtc atcctgccct cgttctccgc
    21181 gagggtgagc gagcccgtcg gccggaagtg gacgtcgacg cccgtctcgt cggcgagtcc
    21241 ccggtacaga tccacgccgt agttcgcgag ctccgtcatc ggatgggagc cgcggacccg
    21301 ggcgacgagg cccgcggcat gccacgaagt tcccgaggcg atccgagctc gctcgaggag
    21361 cagcacgtcg gtgattccga gtcccgccag gtggtaggcg atcgacgacc ccgcgatccc
    21421 accgccgacg atcacgaccc gtgcccggct cgggagtggt ccgtcggcag cctcgggagc
    21481 gaacgaggtc gcggtgagag tgtcgtccaa catccgctcc gtcatgaggc ccgaaggata
    21541 ccggcgctcc acccgcctcc cggttgcctc gggtcgagcc ggttgggacc atgctggcgt
    21601 gagtgaggcg ggaacggcga tgcgggaaga gcggcggttc gtaacagcgc tgttcgccga
    21661 tctcgtcggg tcgacggccc tcggcgaacg cctcgagcct gaggagctga agctcgtggt
    21721 cggggacgcc atcggccgga tcgtgggggc ggtcgaggcc ttcggcggca cgatcaagga
    21781 cctggcgggc gacggtgtgc tcgcgctctt cggcgccccg gtcgcccacg aggacgaccc
    21841 cgagcgagcg ctcctcgccg ggctgcggat cgtggaggag atgggcgagt tcgccgacga
    21901 ggtctgccgg agctggggga tcgacggctt cggcgtccgg gtcggcgtgg agtccggccc
    21961 cgtggtggtg ggggccgtcg gtgcgggttc gcgcgtggag tacggggcga tgggcgacgc
    22021 cgtgaacgtg gcggccaggc tccagacgca ggccgatccg ggcacgatcc tcgtcgggga
    22081 gggtaccgcc gctcgcgtgg agcctctctt cgagtgggga cctcccgtgc agctagagct
    22141 gaaagggaag tcggggacgg tcgccgcgag atccgtgatc gctccgctgg cgacgtcggt
    22201 gtcgacacga gggatcgagg gcgtgcaggc gcctgtcgtc gggcgggagc gcgagctcgc
    22261 ggtcatccgg gaactcgcgc gagccgccct cgagggcacc ggtgcgatcg cagtcatgac
    22321 cggcgagccg gggatcggca agaccaggct cctctcggag ttccgcgaga tgttcgtcga
    22381 gggctcggcg ccgcaaggtc gcccgctgtg gctcgagggc aagtgcgtgt cctacggcga
    22441 gtcgatgacc tattggccgt ttcgagacct cgtccgttcg tggctcgggg tcctcgcgga
    22501 cgagccggag atgcgagtgc gcgtggccct ccgacgcaac gtcgagcggc tcttcggcga
    22561 tcgcgcctct gagctcaccc cgtatctcgc cgcgatgctc gggttgacgc tcgagccgga
    22621 cgaccaggcg cgtctcggtg agctctcccc ggaggccctg cagtaccgga cgttcgaggt
    22681 cgtgaggcat tggctgcagc gcctcgccga agagggtccg gtcgccgtcg ccctcgagga
    22741 tctgcactgg gccgacgcca cctcgttgca gctcctcgaa cggctgctcc ccgacaccga
    22801 ggactctgcg ctcctcctcg tcctcacact ccgtcaggag cgcgaccacc ccgcctggcg
    22861 gctcaaggac gacgctgcca gagagcttcc gcaccgcacc accgagatgg cgctggaggc
    22921 gctctcgggt gatgccggcc tcgacctgct ccgttcgctc gtcggcgcgg gcaccttgcc
    22981 cgaggacatg gagggacgga tcctcgaaca cgccgacggc aacccgttct tcctcgagga
    23041 actggtccgc tcgctcgccg acgcgggttc gctggttcgg gacggcgacg gctggcggtt
    23101 cgatcacgag gccaacgtcg agatcccgcc gacgatcgag aaggtgatcc tcgcgcgcat
    23161 cgatcgcctg gatcctcagc cccacgccgc gctgatggcg gcggcggtcc tggggcgcga
    23221 gttcgggctg ccgctcctcg aggccgtgac cggtgatggc cacgtgaagg actccctctc
    23281 gtccctgatg cgcctcgatc tgctgcgaga ggcgcgacgg tggccggaac cggaataccg
    23341 gttcaagcac gcgttgatcc aggaggccgc ctaccggacg ctcgtcgtcg acgagcggcg
    23401 ggcgctccac gcgaaagccg cgacgtggct cgaggaccgc tacgccggcc gcgaggagga
    23461 ggtcgccggc ctgctggcgc atcattggct cgccgcggcc gacgaggaca aggcggtcgc
    23521 gtacctgacg atggccggcg accgcgcgcg gcaggagtac gcgctcgacg aagcgatcgc
    23581 cagctaccgc gagctgttgc cgatcctggg agaacgcggc gagcaggacg ccatcgcact
    23641 cgttctgttc aagctcgcgt tggcgttcca catgtcgctc cgcttcgcgg aggcgaacga
    23701 gatgtatcag cgggcgttcg atctgtggcg ctggccggat ccggcggccg agcctccctc
    23761 cgcgacgctg cgcgtgggca ccagcttcct cccgaacgac cccgaccctc ggtcggcgat
    23821 cgcgtggccg aacatccagc tctgcatgca gctgttcgac cggctggtcg aggcgtggcc
    23881 cgagcggacg atcgtgccct cgctcgcgga gcggtgggag atctccgacg acggcctccg
    23941 ctacgtcttc cacctccgtg aggatctgac gtggtccgac ggagagccct tgacggcaca
    24001 cgacgtggaa tacgggatca aacgcgtgct gaaccctgac gcaccggggt cgtcggtcgc
    24061 gatctacttc gtcctcgaac acggacagga ccattacctc cgcaccaccg aggatccgga
    24121 tctgatcggc gtgcgtgccc tcgacgaccg cacgctcgag ttccggctcg cggcgccggc
    24181 cccgtacttc atgagcgtga tgaaccgccc cgacggaggg ccgcaaccgc ggcacgcgat
    24241 cgaacgcgac ggggatgcgt ggacggagac cgggaagcag gtggtgagcg gcgcgttcga
    24301 gatcgtggaa cgcgagacgg accgactcgt cctgcggcgt cgtgaggaga cgggctctcc
    24361 acggcccggg aacgtcgaga cggtcgagta cctgcggcgc gagatccccg aggcgatcgc
    24421 cgcgtacgcc cgcgacgagc tcgatgcggt caccgtacgc tacacgccga agaccgccga
    24481 tctcgtgccg ggggtctcgg acgacgcgca tctcggcgcc gcgagctggt cgggattcct
    24541 cgcgttccgg cacgccgacc ccaggatggc gaacgtcgaa ctccggcgag cgctcgcgac
    24601 cgcgatcgat cgggggacgt tggccgagca cgtgccttcg aacctcgtgg tcgccaacgg
    24661 gggcatcgtg ccgccggccc tgcaggggca cacgccggac atcgtccccc ggttcgatcc
    24721 ggaagccgcc cgcgagcatc tccggcgctc gggcctgtca cgagacgagc tccagggcct
    24781 cgagatcgcc ggcatcgaga cgtggctcga cgacttcctt ctcgtcgtgt ccggaacctg
    24841 gaaggaggtg ctcgatctcg atgtgcccgt tcggccctgg acgctggagc aggcgctctc
    24901 gatcggagat ccgacggaga tggccccgat cgtgatcacc ggatggctgc ccggctacgc
    24961 ggaccccgag tacttcctcc gcctgctgtt ccaaaccgac agcaagacca actacggtgg
    25021 gttctccgat ccggagttcg accagctgat cgagcgggcg cgccaggagc gcagcgaccg
    25081 ttcgcggctc gagctgttcc acgaggccga tcggatggcg gtcgccgacc ggatcgcgtg
    25141 catcccgctc gtctacggcc ggagcatgtc cttcgtgaag ccctgggtca cgggatggtg
    25201 ggagttcggg aagtcctcgt catcggtcgc ggacctgatg acggcgagtg ccacgtcgag
    25261 cgctcgctga cggctgtacg ggttcgcggt ctaactggag tggttgagtt cccgggcggc
    25321 cttcgcggct tccctcgccg cctcgcgctc ggccttggcc gcgtcaagcg cagcttcgcg
    25381 ctcagccttc gcggcctcgc gctcggcctc ccgctcggcc ttccactcgt cgtgggattc
    25441 cttgcgctcc atgttcgcga cgaggcgctc gagcgcgttg accaggcccg gagcgttctc
    25501 gttcttgagg cagttggcga gcacgtgcgc gatcgcgttc tcgagaccgg tcagcttcac
    25561 cggttccgtg tcgttggtct cctcgacctc gccgccttct tcgtcgtccg gggtcgcagc
    25621 cgcctcgcat gcttcggcgc gctcgaggac gacctcatca cctgactcgg tgtcgtctgt
    25681 gccttcggtc tcgtccgact ccccgtcgtc cgtggcttcg tcgtcgcatc cctcgacggg
    25741 ttcctcgccc tcgaccggct cctcgccctc gaccggctcc tcgccctcgg tgccttcggg
    25801 atcctcgatc gtgtctccgt cgtcaacgac ctcgtcggtc ggctcgcagg tggaggccgg
    25861 ctcgtccgca gggtcgtcga aggcgtcacc ggcgagtgcg aatccggcgg agagcaccgc
    25921 gatgaccacg cctacgatgg cgacgaaacg caggcgctct cctttgtgca tgccccagta
    25981 tcgaccgatc cgggacgaaa ccttgagccc tcgttcaggt atgcgtcgat caccgcccag
    26041 agtgctttcc cgtgatctcg ctcgacggca tccggcgcat cccggtcgcc tgaccctcac
    26101 gagatcgtga tgcggccgtc ctctcctatg gggaagctgg ggttgtaggc gatctcccac
    26161 gggtgcccgt ccggatcggc gaagtagccc gagtagccgc cccagtctgt gtgctccgcc
    26221 ggcttcagga tgcgagcccc agcggccacc gccgcttcga aggcggcatc cgcttccgca
    26281 tcgctcccga cgttgatcgc gagggtgatg ccgccgaact ccgccgtcgg ctccgatcgc
    26341 agaccggcat ccttcgcgag gtcctcccgt ccgaacagtc cgagatacga gtccgacgtc
    26401 cggaaccagc agatctcgtc gccgacggag cccgcgagct cccatccgag cgcctcgtag
    26461 aacaccttgg atctcgccac gctgtcgacg ccgagcgtca cgatgttgat ccgagcgggc
    26521 acgctcatgt ggacctcctt tctcgcatcg acgttatcgc gcgcgcccga ttcggtcgct
    26581 ctccgtagag ttggcccatc gatgcctgcc gttcgactga acccgatgct gcgcgtgatg
    26641 tggaagctgc atcgcttcgc cctccgtgtc tcgggcggtc gcgtcgggtc gcggatgggt
    26701 tcgatgatgg tgctcctgct ggagaccacc ggacacagga gtggccggcc gcggacggtc
    26761 ggcctctccc atctcgagat ggacggccgg tacttcgtga tggcgtcgta tgccggggag
    26821 gatcgcgatc cggcctgggc gaagaacctc cgggcacatc cgcgggcgac ggtgacgctc
    26881 cgcggcaggt ccaccccggt ggtcgcacgg gcgctcgaag gcgccgagca cgacgctctg
    26941 ttcgaccggt tcgtggaggc ggacgcctcc tatcgagagt accgagagcg gacgacccgc
    27001 gagatccccg tgttcgaact gcgcccggaa ggtgcgtgag ccggttgccg tccatcaccg
    27061 acatcgaggg cctcacggtc ggtcattgga ccgacgaggt tgcgctcacc ggttgcacgg
    27121 tggtcttgcc ccctccggga acgatcgcct cctgcgaggt ccggggaggt gcgcccggga
    27181 cgcgcggcac ggacatcctg cagcccggca cgatcatcga ggtcgcgcac gcgatcgtcc
    27241 tgaccggcgg gagcgcgttc ggtctggcga gcgcgggtgg agtggagcgc tacttcgagg
    27301 agcgggggat cggaagcgag atcggtccgg tgcgcgtgcc cacggtgccc gccgcggtga
    27361 tcttcgacct cggcgtgggg gatccggctc gacgaccggg cgcggacgag ggctacgcgg
    27421 cgtgcatcgc cgcatccacc gatgtcgccg aggggcgggt cggggtcggc accggcgcca
    27481 cggtcgcgaa gctctgggga cccgagcgag cgctgccggg aggagtcgga acctgggcgg
    27541 tgcgcgacgg cgagctcgtc gtgggcgcgc tggtggtcgt gaacgccgtc ggtgagatcg
    27601 tggacgagga cggatcgatc ctcctgggtc cgaggctgga gcccggcgag cggcgcgagg
    27661 acctggtgga gcagctcggg tcgtcggcag cggccaacac gaccctcggc gtggtggcga
    27721 cgaacgcggt gctgtcgaag gaggacgtcc gccggctcgc gaccgtaggg aacgatgcct
    27781 tcgacgtcgc gatccgaccg gcccacacga tctacgacgg cgacaccgtc ttcgcgctgg
    27841 ccacgcagca ggtcgatgcg agcttcgagc aggtcgccgc atcggtcgag gccgcggtca
    27901 cgacctcgat ccgcaggtcc gtcatccgcg gcgatggttc aggagcggct ggcgcctaga
    27961 cacgtggtgg tgccagaggt gcatcacggc gtaggcgcgc caggggcgcc agcgttcggc
    28021 tcgcgcgagc atcgccgtct gcgtggactc cagaccgaga gcctcgaatc ccttgcggac
    28081 accgaggtca ccggccatga acgcatccgg gtcgcggagc gcgcgcatcg cgacgtaggc
    28141 gagggtccac ggtccgaccc ccgggacttc acccagtctc ccgagcgtct ctccgggcgg
    28201 tgcgtcgccc gagagatcga gctcaccgcc cgcgaccagc gcggcaactc ggcggatcgt
    28261 ctcggctcgc gaccggggca tcccgaacgc ctccggcggg agctcggcca tgccctccgc
    28321 ggtggggaag agatgggtga tcgccccgct cggtcgttcg aggggcgttc cgacggccga
    28381 caccagcctg ccgagagacg tcctcgcacc agcgacggac acctgctgtc cgaagatcgc
    28441 ccgcacgacg agctcgaagc cgtcgaacgt gcccgggacg cggatcccgg ggatccgggc
    28501 caccagcggc atcatcatcg gatcggaggc gagcgcggcg tcgatggcag cggcgtcggc
    28561 gtcgaggtcg aacgctcggc gagcgcgatc gcggagatcg tcgaggtcga gggagccctc
    28621 cgacgaggcg cggaaggcga cgttgtccga gcgaggcgac atcgcgacca ccgcggcccc
    28681 gctcggatgc cgcacggatc gcacgtacgt accgtcccgc gtctcctcga tgccggggac
    28741 ggcgcgcgcg ccgaggaacg acaggagcgt gctcgccgcg aacggcgcct ggaagaggag
    28801 tcggaaggat cgagtgtgta gcccgtcgtc cacgccggca ggtatagcgg atggtctgtc
    28861 ggtgccaccg gctaccgtcg cacgcctccc tctcctcgac aggaggagac gatggatcag
    28921 gtagcgaggg tgatcctggc gctggaggcg ccggaggtgg ccgaggaggt catgcacgtc
    28981 ctcgatcgga gcggccgtgc gcgagtggtg gctaccgccg gcgacgatcg ccagctcgcg
    29041 gaggccgtgc gccagctcga gccggacgtc gtcgttgccg agcccagcct gctctccggt
    29101 gcgagagcgt gcggcgtggt gctcgcggtc gacgtccgcg agtcggtgag ttcacttcgg
    29161 acggcgatcc gagcgggtgc cgcaggctat ttcgtgtggc ccaaggaacg cgacgatctg
    29221 ctcgtggccg cggcggccgc cgtccggaga gtgcagcagg cggccacacg aggcgcggtc
    29281 gtcgcggtgc acggtggtcg cggtggtgcg ggggttacgt tcgtggccac gcacctcgcc
    29341 gccgcgttcg ctcgccgcgg gtcggcgatc ctgatcgacg ccgaccccgt cttcggcgac
    29401 gtcgctcagg cgctgggtgc gcccacgggg gaggaagccg aacctgccca caccttcgcc
    29461 gacgccgccg ccctcggcga agatctcggt ccggagcagc tgaggagcgc gctgtggcgg
    29521 cacgagagcg aagtggatgt cctcctgcct cctccgccgg aggaggcgat ccggctcggt
    29581 ccggaggatc tgcgtctcgt caccgacgtg gccgcgggtg ctgcggacgc cgtggtcgtg
    29641 cacctgccgc gcgcgctcgg ggcgatgacg cgcgccggtg ccgagacctc ggactcgctg
    29701 gtcgaggtcg tcaccctgga cgtcgtatcg ttccgggcga cgtcccgcgc gcttgaggcg
    29761 tgcgcgcccc tgcacctcga gggacgcgtg agcttcgtcg tcaacaaggc ggctcgcagc
    29821 gagatcacgc cgggagacgt gcatcgcgtc ttcggcgaac ccgcccgcgc ggtcctcccg
    29881 cacgaccgcg ccgtccgctc ggcgcaggag cgcaaccggc tgctgcctcc gaaaggccgg
    29941 atggcccatc ggttcgatcg cctcgcggat gccctctcca cccccgtcgt cgaagagatg
    30001 gcgtcctgac ccggctacgc ttcgcccccg ggctacgctt cgccgatgga cgaacgggtg
    30061 ctgctggtcg aggacgaccc ctcgatccgg gagatggcgg gcctcgggct cggcaacgcc
    30121 ggcttccggg tgacgacctg cgcggacgga cgggagggac tcgagcgtct ccggcgtgac
    30181 cccttcgacc tggcggtcct ggacgtgatg ctgccgtcgc tcgacggctt cgagatcacc
    30241 cgcgaggttc gcaaggacag ccggatcccg atcgtgatgc tgacagcccg cgccgatgcc
    30301 accgacgtga tcgtcgggct cgagctcggc gccgacgact acgtgacgaa gccgttcgac
    30361 atgcccgtgc tcgttgcgcg ctgccgggcg gtgctccgcc gggccgctgc cgaaccggcg
    30421 gaccctgcgc tgcgcgtggg tgacctggag atcgacgtcg gtgggttcgc agcgcggaag
    30481 ggcgggcgcg atctcgcact cacggcgacg gagtttcgac tcctcgtcga gctggcgcgc
    30541 cggcccggcc aggtgctcac ccgcgacgtc ctgctcgctc gggtctggga ctacgagtac
    30601 ctgggcgact cacggctggt cgacgtcgcc gtccagcgtc tccgcgggaa gatcgaggac
    30661 gatcccgcgc atccccgtac gatctcgacc gtgcggggag tcggctaccg gctcgagcgg
    30721 gttggagcgg agtgaccgga ggacgcggat cgcctggccg gctcgtgctg gtggtcgtcc
    30781 tggtggccgc cgtcgtgtcc gccgcactcg cgacggccgt gttcgtgctg gtccgcgacg
    30841 cccgtttcca cgattcgctg gagcgcgctc gaaccgaggc cgtcttcgac ctcacgatcg
    30901 ccggtcccct cctccaaggg tccggcgaac ccggggcggt cgtcgagacc ttccgggagg
    30961 agcgcggcat ccacgccatc ctggtcgagg acgggcggac gatcgacgcc gacccggcct
    31021 ccgggatctc catcccgccc gagctccgcg cgatcgcggc gcgagggcag ctcggctacg
    31081 atcggatcga ggcaggtgga gagcctctcc tgctcgtcgg cggcccgacg cccgatcgcg
    31141 gtgccgagct ctacttcgcg ttctcggaga ctgggatcca cgaggacctc cgtgagctca
    31201 tgacggcgct cgcgatcggc tggatcgcga tcgtcgtggt cgcgttcgcg ctcgcgcgtg
    31261 tggcggcgag ccgcatcgcc tccctcgcgg aagccgaggc atggggacga cgtttcacgt
    31321 ccgacgtctc ccacgagctg cggacgcccg tcgccgccct cgtgtccgag gcgtccgtgc
    31381 ttcgggagca tctgaccgcg atgccgcccg acgcgcgacg ggccgccgag ctcctcgtcg
    31441 gcgacgtcgc gcgcctgcgc cggctcgtcg aggacctgac gaacctcgct tcgctcgacg
    31501 cggggcgtga gcaggtgcgc acggaaccgt tcgacctggc gaccctcgtt accgggaccg
    31561 tccggagccg aggatgggcc ggggaggtcc agatccgctc ggatcccgtt cccgtcacgt
    31621 cggatcggac tcgcgtcgat cggatcgtcg ccaacctgat cggcaacgcg ttggaccacg
    31681 ggggaggagc ggtcgtcgtg cacgtgagag ggagcggcga tggcgcctca gtcgaggtcg
    31741 ccgacacggg cccggggatc gccccagagc acctcgagca cgtcttcgat cgcttctatc
    31801 gggccgaccc gtcgcggtcg ggccccggaa gcggtctcgg cctcgcgatc gcccgcgaga
    31861 acgcgcggct gctcggtggt gacgtcgagg cacggagcga ggtcggtgtg gggtctcgct
    31921 tcacgctccg gctccccgag gatcctgttg ccgaaccgtg acacccgcgg ggttcccgtg
    31981 ttgccgtcga cgcagagcat gggtcgtgtt cccgacccga ggaggcacac atgaagcgcc
    32041 tgatcgcgat gatggcggtg cttgccacgc tcgcgaccgc gtgcgccgag agcggtgcac
    32101 agccgctcgg ccccgctccg agtgcggacg ggccgagccc gtcgccgggc tcaccgtcgc
    32161 ccacgctctc gcccacgtca ccggaaccgc caacccccgc gcagtcgatg acgtacgagc
    32221 tctggttcaa ctacgacggc tcgctcttcg tgacgcatcg gactgagccg ttcacgccct
    32281 ccgtgggcac ccgggcactc gaagcgctgc tggaaggccc ctcggcgccg gaacgtgcgg
    32341 cgaacctcgg gaccacgatc aacgccggga cccgactcaa cggcctcacg atcgacgacg
    32401 gcgtcgcgac cgtggacctc gatgccacgt tcgtcggcga ggagacgcct gcgatcgccg
    32461 tggggagctt ggcgcagatc gtgttcacgc tcacgcagtt cgatcgtatc gggggtgtcg
    32521 tgttcgaggt cgacgggggg cccctcacga acttcggcgg ctacgagatc gatggcgcgc
    32581 agcggcgcac gaacttcgcc gaccagctgc cggcgatcct cgtccagagt cccgggatcg
    32641 gtgagcgcgt ctcgagcgcg gtcctcatca gcgggacggc caacgtgttc gaggccgtcg
    32701 tgtcgatcgc gatcctggac cagaacggga aggtgatcgc ctcgacgttc acgatggcga
    32761 cctgcggcac cggatgccga gggacgtggg agacggacgt cgcttacgag gtcgccgcca
    32821 cccagcccgg cacggtccgc gtgtacgagg tcagcgcgat ggacggctcg aagatcaacg
    32881 tggtgaacat cccggtcacg ctcaccgtgt gaccgggatg cgggagcaga gggcggcctc
    32941 acggccgccc tcttcctctt cacccgactc gtgccttcct cacttcctcc ggctggccgc
    33001 cttcgtgcga cgctgtccca ggcgtgatcg cgcggcgacg aatcccccga caggttcgag
    33061 gaaccccccg cattggggat ggttccttgt cggagttcca cgctagggtc gttccatcga
    33121 cgcggcgagc cttcctccgc cggtcggcac ctcctcatcc ccctcgtgtc ggtgcgtgct
    33181 tccccgcgag tgttcggagg gaacctggtg gaccacggac ggcgagtcat cctcgcgctc
    33241 gtcgcatgag cgccgatcct ctcgccaagg tgcggcgaca gctgcgggcc cagctgcgcg
    33301 aacgggtcga tgcccgtggg ctgtccgcgg cgccgcgaac ggagcggcgt cttcgcgttc
    33361 gtgaagaggc gctgtccttg atgcgcgaga gcggcgcgct gctcccacag cgggatctcg
    33421 cgcgcgtggt caacgaagtt tccgacgagg tcgtcgggtt cggtcccatc gaattcctcc
    33481 tgcgggaccc cggagtgacc gaggtgatgg tcaatggacc tgacgacgtg tacgtggagc
    33541 gggaggggcg gatcgagagg gtggaggacc ggctgtttga gggggaggag tcggtccttc
    33601 atctgatcga gcgcatcgtg ggtcccctg
//
LOCUS       DASUYD010000056         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_469869, whole genome shotgun sequence.
ACCESSION   DASUYD010000056 DASUYD010000000
VERSION     DASUYD010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469869"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            829..1152
                     /locus_tag="VF195_03110"
     CDS             829..1152
                     /locus_tag="VF195_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579846.1"
                     /translation="MVRGPDCRRPDRGKRLSGRLPDPRGGAGDEAYPVDDLDGHCTTA
                     MLNVPVQGVGDVEQHLAVEIRLTAARSASSEPPPFVALTTSLPSRAASPKQPGSLDVT
                     STIEP"
     gene            1149..1838
                     /locus_tag="VF195_03115"
     CDS             1149..1838
                     /locus_tag="VF195_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019329973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579847.1"
                     /translation="MKTRILLVEDEATISEPLAESLGRDGFEAEVTPTLAGAREAIRR
                     EVPDLVLLDVMLPDGDGRDLAREIRKESDVPIVMLTARGEEIDRVVGLELGADDYVVK
                     PFSSRELTARIRAIMRRGRAREQRSPIEIGALRLDPTSRTLTKDDESIELAAREFDLL
                     HLLMTNAGEVLRRERIMDDVWDPHWFGPTKTLDVHISWLRKKIEDDPSHPTYITTIRG
                     VGFRFRSDAET"
     gene            1911..3218
                     /locus_tag="VF195_03120"
     CDS             1911..3218
                     /locus_tag="VF195_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6579848.1"
                     /translation="MGVPLTVNLQRRATSELKTQALIQAQGIAAQIGVENIDDPARLD
                     QIVTQGAAQVGGRVIAVDDGGFLVADSDGSASLDTLYATPGRPEIRHALAGTPFSEVR
                     DSVDLGHDIMATAVPIQDEQRLVGAVRITRSVQEVNDNIRNVTLGLAAIGVAALTAGM
                     LLAFGLAGSLSRPLTRLAAAARRLGEGDLSARAGNVSGASEVEELGESFDEMADRLER
                     SVQAQREFVANASHQLRTPLTAMKLRIEGAIAEAPDEAMRQRLEAADLEVDRLSEIVD
                     RLLVMAREIEEGISASVDLGGAVHRTVLRWNDRARQRDSTVVARGDGGLARVDPTDLD
                     QILDNLLDNATSYARGEVTVEMGGSNGTVFVAVRDRGPGIAPQDLAHVTERFYRGLGV
                     PSGGSGLGLAIARQLAEKWGGTLNVESAYGEGTRVEVRLDAAP"
     gene            3260..3637
                     /locus_tag="VF195_03125"
     CDS             3260..3637
                     /locus_tag="VF195_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579849.1"
                     /translation="MNRKTVKAIALGLGGLVVALLLTVGAFALAGQQIAQPAGVPIFT
                     TTPSPSPDHDRTETTFPEHERTETPSPSIDDQGGGSGDSTPGSSSGSDDGGDSGGHDS
                     SGSDDSGSGSDSSGSGSGEHDDD"
     gene            3736..>3974
                     /locus_tag="VF195_03130"
     CDS             3736..>3974
                     /locus_tag="VF195_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579850.1"
                     /translation="MRKMLRVMAILAAFGLTAWIGVVALAQGSSTPSPPTSTTTSPTS
                     STGSEDVKGSCDEAEHASDPECVGANPVVEDDSEN"
BASE COUNT          676 a         1307 c         1366 g          625 t
ORIGIN      
        1 tacggccttg ggatcttcgg cgcgttgttg tactactggc agcaagccga ctcgttctgg
       61 gagtacgtgt gggcgatctt cccgaaggcg atcttctggc ccgcgtacat ggtctacgaa
      121 gggttcaggg ctctcggagc gtgacgccgt ggaacttccc cgagtcgcac gatttctaag
      181 ggatgcctca ggttgcgcgg agagcccgga tgcggcgtcc gagccctgac ccacacccac
      241 atcgtcaccg cgaagagcgc gagcaacatg acgtcgatca gcagtaatcc ggcttggatg
      301 gtcgggaatg gtccccggaa tgaatgacca gcatgcccgc cgtgtcagcg gcgtgacggc
      361 gatcgcccgc cgggccttcc gttgcctgtc acggcaccca ccgccgctaa ccatgagggc
      421 gtggaagccg cgtccttcga cctcgacaag acgatcatct cgcgctcgtc ctcgctcgcc
      481 atgtctcgac cgatgtaccg gggcaggcat ggtcatgcgc gggcagctcg tgcgcggcgc
      541 gtacgcgcag ctcgtggacg cgctggtcgg ggccgacgag aagaagatgg atcggctgaa
      601 ggaagggatg ctccagctca cgcgcggatg ggaccggcac gaggtcgagt gcacgagggg
      661 gtggcccagg aggttgcgga gatgcaggtt gtggattcgg cggtgcctgt cgcgggtcct
      721 tacaacgtca tcgcgatcgt ggaggccgcg ggcacggacg ccttcggcaa gacggtggtc
      781 tcccggtagt ccgccggatc tcctcgaccg ttgcctcgcc ctagcgcggt ggtgcgcggc
      841 ccggactgcc gtcgccccga tcgcggcaag cgcctgagcg gtcgccttcc cgatcccaga
      901 ggtggcgccg gtgacgaggc atacccggtc gacgatctgg atggtcactg caccaccgcg
      961 atgttgaacg tgccggtcca gggcgttggc gatgtcgagc agcaccttgc cgtcgagatt
     1021 cgcctcaccg ccgcgcgtag tgcctccagc gaaccgccgc cgttcgtcgc gttgacgacg
     1081 agcttgccgt ctcgggccgc ctcgccgaaa caacctggaa gcctcgatgt gacgagtacc
     1141 atcgagccat gaagacgcgg atcctccttg tcgaggacga ggcgaccatc tcggaaccgc
     1201 tggccgagag cctcgggcgt gacgggttcg aggccgaggt gacacccacc ctcgccggag
     1261 cccgagaggc catccgccgc gaagttcccg acctcgtgct gctcgacgtc atgctcccgg
     1321 acggggatgg acgggacctc gcgcgggaga tccgaaaaga gtccgacgtc ccgatcgtga
     1381 tgttgaccgc tcggggcgag gagatcgatc gcgtggtggg gctcgagctc ggagccgacg
     1441 actacgtcgt caagccgttc tcatcgcggg aactcaccgc tcggatccgg gccatcatgc
     1501 ggcgcggtcg cgcccgggag cagcggagtc cgatcgagat cggggcgctc cgcctcgacc
     1561 cgacgtcgcg cacgctcacc aaggacgacg aatcgatcga gctcgccgcc cgcgagttcg
     1621 acctgctgca cctcctgatg acgaacgccg gcgaggtctt gcgccgggaa cggatcatgg
     1681 acgacgtctg ggacccgcat tggttcggcc ctaccaagac actcgatgtg cacatctcct
     1741 ggctccgcaa gaagatcgag gacgacccct cacacccgac gtacatcaca acgatccggg
     1801 gggttggatt tcggttccgc tcggacgccg agacctagga ggcgaccacg aaggtccgaa
     1861 cgaggctcgt actcgccttc gcatacatcc tcgtcgtcct gatcatcgcg ttgggagtcc
     1921 cgctcaccgt caacctgcag cggcgtgcga cgtccgaact caagacccag gcattgatcc
     1981 aggcgcaggg gatcgcggcg cagatcggtg tcgagaacat cgacgatcca gcccgcctcg
     2041 accagatcgt cacacagggg gccgcgcagg tcggtgggcg ggtgatcgcg gttgacgacg
     2101 gggggttcct cgtcgcggat tcggacggat cggcgagcct cgacactctg tacgcgacgc
     2161 ccggcagacc ggagatccgt cacgcgctcg cgggcacacc gttctctgag gtccgcgaca
     2221 gcgtcgatct cggccacgac atcatggcga ccgccgttcc gatccaggac gagcagcgac
     2281 tcgtgggcgc ggtccggatc acccggagcg tgcaggaggt caacgacaac atccgcaacg
     2341 tcaccctcgg tctcgccgcg atcggcgtcg ccgccctcac ggcggggatg ctcctcgcgt
     2401 tcggactcgc cgggtcgctc tcccgtccgc tcactcgcct tgccgcggcg gcgaggcgcc
     2461 tcggtgaggg cgacctctcg gctcgagcgg gaaacgtgag cggcgccagc gaggtggagg
     2521 agctcgggga atcgttcgac gagatggccg accggctcga gcgctcggtc caggcccaac
     2581 gcgagttcgt cgccaacgct tcgcaccagc tccggacgcc cctgaccgcc atgaagctcc
     2641 gtatcgaggg cgcgatcgcg gaagctcccg acgaagcgat gcgacagcgc ctcgaggcgg
     2701 cagacctcga ggtcgatcgc ctgtccgaga tcgtcgatcg gctcctcgtg atggcacggg
     2761 agatcgaaga gggcatctcg gcgagcgtcg acctcggtgg ggccgtccac cgaaccgtcc
     2821 tccgctggaa cgaccgagcc aggcagcggg actcgacggt cgtcgcgcgc ggggacggcg
     2881 ggttggcccg cgtcgatccc accgatctcg accagatcct cgacaacctg ctggacaacg
     2941 ccacctcgta cgcgcggggc gaggtcacgg tcgaaatggg cggttcgaac ggaacggtct
     3001 tcgtcgccgt tcgggaccga ggacccggca tcgctcccca ggacctggca cacgtcaccg
     3061 aacggttcta tcgagggctt ggggtgccct cgggcgggtc ggggcttggt ctcgcgatag
     3121 ctcgacagct cgccgagaaa tggggtggca ccctgaacgt ggagagcgcc tacggggagg
     3181 ggacacgggt ggaggttcgc ctggacgccg cgccgtgacc tttacctccc cttagccaag
     3241 gccgccgtag gctcgagcca tgaacaggaa gaccgtgaag gcgatcgccc tgggcctcgg
     3301 cggactcgtc gtcgcgctgc tgctcacggt gggcgcgttc gcgctcgccg gtcagcagat
     3361 cgcccagccc gccggcgtgc cgatcttcac gacgacgccg tcgccgagcc cagaccacga
     3421 ccgaaccgag accacctttc cggaacacga acgcaccgag actccgtcgc cgtcgatcga
     3481 cgatcagggc ggaggctccg gcgactcgac gccgggaagc tcgagcggat cggacgacgg
     3541 aggagactcc gggggtcacg actcctcggg ctcggacgac tcggggagtg gttccgattc
     3601 gtccggatcg ggctctggcg agcacgatga cgactgaccc agatccttta cttcctcttg
     3661 gccgtccgat gctcgggagg taactgaagg ctctttaggg tggataccga taaggacttc
     3721 gagaggaggg ctttgatgcg caagatgctt cgagtgatgg cgatcctggc tgcgttcggg
     3781 ctgaccgcgt ggatcggcgt ggtggctctg gcgcaaggat cgagcacgcc gagcccgccg
     3841 acgtcgacga ccacgtcccc gacgtcgagc actggcagcg aggacgtgaa gggcagctgc
     3901 gacgaggccg agcacgcgag cgaccccgag tgcgtcggcg cgaacccggt ggtcgaggac
     3961 gactcggaga acga
//
LOCUS       DASUYD010000057         5358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1791778, whole genome shotgun sequence.
ACCESSION   DASUYD010000057 DASUYD010000000
VERSION     DASUYD010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5358
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1791778"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VF195_03135"
     CDS             complement(<1..770)
                     /locus_tag="VF195_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6579851.1"
                     /translation="MRHHNPKLPLVLHLAPASVDLVHQVFLRIEQPEEPGARPEADRY
                     ETLAQFYEAIVGALARLGDEVFTGDPRAQVTKGYAGHGGGTLFAVTDLDSALLAIEVI
                     VEQGEGDPHVGARSGRLRRHDSPDASQLEPFGFQGAPERAHYWRFLDIVEGRTPLGAV
                     HPMRLDPSEDDLPDGELRGLSRLFDACYSLQMNVMERVWGVGDESPLIDSVPLMNHAE
                     KPIAVALLREAWPDGSGEVAGPPFAWDPMPLDDMRRLAR"
     gene            1552..2430
                     /locus_tag="VF195_03140"
     CDS             1552..2430
                     /locus_tag="VF195_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579852.1"
                     /translation="MTYEILNGWIVGKLPGPNGVLIPVKHFPQGPYERRQRNKPLLCL
                     HTTETDGYVEDLRFPTEFQVGEEVIGQHRPLWARGAAVDEHDHDLLQIEIVGFSKLDR
                     WLPKPSSLSPLVALMALLHRRGFVVTALRRPTRWPVPLDRLPAAVDTYYRRLEVWDHA
                     FVYGHVEIPGDEHWDPGSLDHPVLFSMVRNVLAPKEVDGEMLSDNQLEGIQFANGMRR
                     YLDGPENEPAEPGPHRQGFRFAQRITTHIEGEPPPAAFEPNRSSTEGMQLSPDEKHPG
                     RQEETQSRGRSEGSSD"
     gene            3243..3677
                     /locus_tag="VF195_03145"
     CDS             3243..3677
                     /locus_tag="VF195_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579853.1"
                     /translation="MRRDVLALLVVLVLGGSGVATAAVFAGAGSPSGGSPGRSDKAEA
                     IEKDSGIHGGPIERFHLAGSCDLTAVGSLPGNWTHGDYVSAVAAGGEAAEIREAARSD
                     CGKPMVAVGHGGGPAEHALEHMAAGQAHTGKGDDSGSDTVGS"
     gene            complement(3800..4126)
                     /locus_tag="VF195_03150"
     CDS             complement(3800..4126)
                     /locus_tag="VF195_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579854.1"
                     /translation="MQIVYVMEVIDPLESRNGARYVRRFTYETREQAWARYSEAIRFG
                     LEAYVDEVGRMHVTTQTTAQEHLSRSSVAAWRAPRDSAVGERRRSGERSNPGGGALGL
                     SGSANP"
     gene            complement(4825..5063)
                     /locus_tag="VF195_03155"
                     /pseudo
     CDS             complement(4825..5063)
                     /locus_tag="VF195_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010948855.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(5219..5294)
                     /locus_tag="VF195_03160"
     tRNA            complement(5219..5294)
                     /locus_tag="VF195_03160"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5259..5261),aa:Asn,seq:gtt)
BASE COUNT          921 a         1783 c         1781 g          873 t
ORIGIN      
        1 cgtgccaaac ggcgcatgtc gtcgagcggc atcgggtccc aggcgaacgg tggcccggca
       61 acctcgccgg acccatcagg ccacgcttcc cgcagcagcg ccaccgcgat cggcttctcg
      121 gcgtggttca tgagtggcac cgagtcgatc aagggactct cgtctccgac tccccagacc
      181 cgctccatca cgttcatctg gagcgagtaa caggcatcga acaaccgtga tagtccgcgc
      241 agctcgccgt cgggcagatc atcttccgac gggtcgagcc gcatcggatg gacggcgccg
      301 agcggggtcc tgccctcgac gatgtcgagg aaccgccaat agtgggcccg ttccggagcc
      361 ccctggaatc cgaagggctc gagctgagat gcgtcgggcg agtcgtgccg tcgaagccga
      421 ccggagcggg cgccgacgtg tgggtccccc tcgccctgct cgacgatcac ctcgatcgcg
      481 agcagggccg aatccaggtc ggtgacggcg aagagtgtgc cgccgccgtg acccgcatac
      541 cccttggtca cctgcgcgcg tgggtcaccc gtgaacacct cgtccccgag gcgtgcgagc
      601 gcgccgacga tcgcctcgta gaactgagcg agcgtctcgt agcggtcggc ctccggtcgt
      661 gcacccggct cctcgggctg ctcgatccgc aggaacacct ggtggacgag atcaaccgat
      721 gccggcgcga ggtgcagcac cagcggcagc ttggggttgt ggtggcgcat cggctcggga
      781 tagcgaggga cggcttcgcc cgtgaatcgc ggcttccccc cgccgcgttg aggaggttgg
      841 ctgccaaagc ggcgtgaagc atctcctcgc gagccacgta cttgatcgag gtggcggccg
      901 cggaatcggg atccacgacg ctccacatcg cgtactcgta gggcgcgatc gtgctgtgtt
      961 cgacctcgac cgcccactgg aggtggcggc gcagatcctc gacgtcggcg atcggttcga
     1021 tcatggtccc ggttcccgga tgctatcgcg ccgagatctg cggcgggctg ctgggctgat
     1081 ccacctcgac ctccgtgtct cgccgagatc tcgtcgacat gagcgcgggc gttctgctca
     1141 ccacaagcgc acccgggtgt cggcgaccgg agcggaggcc tgcatcgtga ggccgcgctt
     1201 ctgtcggatc gaggacgaag gggtgaggac caacctcgag ctcggaccga tggtcgtacg
     1261 tacgtcgcga atcccacagg ggaaggccga aggtcgcagg tctcttgaac caatgcttcg
     1321 gctgtaggac cagccgagga tgggcaccgg ggggccatac gctccgtagg gaggacaggc
     1381 cgatacccgg atacacagat gtccccaaca cagggaggct cgaacgtggc cacggcggag
     1441 cgcatcaggg tgctggtcgc ggatgacgaa ctaccatgaa ccggtggaat cgaggggatc
     1501 gccacctctg gaaccacgac cgcaggtgga tcccgcacgc ttcctacatc gatgacgtac
     1561 gagatcctca acggctggat cgtcgggaag ctcccggggc cgaacggagt cctgatcccg
     1621 gtcaaacact tccctcaggg gccgtacgag cggcgccaac ggaacaagcc cctgctctgc
     1681 ctgcacacga ccgagacgga cggttacgtg gaggatctcc gcttcccgac cgagttccag
     1741 gtcggcgagg aggtcatcgg ccaacaccga ccgctgtggg cacgaggcgc ggccgtcgac
     1801 gaacacgacc atgatctgct ccagatcgag atcgtggggt tctcgaagct cgatcgatgg
     1861 ctgccgaagc catcgagcct ttcccctctc gtcgcgctca tggcgctcct ccatcgccgc
     1921 ggcttcgtcg taaccgcgtt gcgacgtcct acgcggtggc cggtcccact cgatcgactc
     1981 ccggctgccg tggacaccta ctaccgtcgc ttggaggtct gggaccatgc gttcgtctac
     2041 ggtcacgtgg agatcccggg cgacgagcat tgggacccgg gttcgctcga ccatccggtg
     2101 ttgttctcga tggtccgcaa cgttctcgcc ccgaaggagg tcgatggaga gatgctgtcc
     2161 gacaaccagc tcgagggtat ccagttcgcg aacgggatgc gccggtacct ggacggcccg
     2221 gagaacgagc ctgcggaacc cggcccgcac cgtcagggat tccggttcgc gcagcggatc
     2281 acgacgcaca tcgagggtga gcccccgcca gcggcgttcg aacctaaccg atcctcgacc
     2341 gaaggcatgc agctgtcacc cgacgagaag caccccggac ggcaggagga gactcagagc
     2401 cgcgggcgca gtgaagggtc ctctgactag cacgcggcca ctccgtgcgg cgcggctcgc
     2461 gctgcccgcg acagcgcgga caacgagcag catctcgctc aacctccccg cgccatatgg
     2521 cgatgagtag gagggagatg ggagaaccga aggactctca agacgaggcc cactgccggg
     2581 tacacggcgc agcttcaggc cccataagcg acgacctccc gtgccctctg ccacatgcca
     2641 ccgccaggtg cccggtctgc tgggtggggt ggatcggtcc tgggcccgac gacagcgtcg
     2701 tgctctgcat ccttactcgg cacatcgtgg agacccaccc gaacacggcc cacgccacaa
     2761 ccctcagatt caacgcgggg cagttgggtg ggtccgtgga tcccgacgac tggtgtcacc
     2821 acgatccgga acagcaccgt cgtgtcacgc tcctggagcc catatcgtga tgtcgtcgat
     2881 ctcgatcagg cgacgacgtc gaaacgacgg ctacccggcc tggtgccgac ccgttcaagc
     2941 gtcgcacctc gctgccaaga tcggcttccg ccggtcgcca tcgggcttag cagactgcct
     3001 aggctgcgat cgcccgatcg gctcaggcgt ccagatgcag ccggaccgtc agtcggcttc
     3061 tcaagcgacg ccgtgaggtg atccgacagg aagatcgtcc gtcgtccatc agccccgatg
     3121 gccgtctcgc agatcgcgca gcgccctacg tcatccggtc ctggccatgg ccgatcggtc
     3181 gattgaccag gggctcgcta cggcccacgc ttgtcggacg agcgcccccg aaggaggctc
     3241 acgtgaggcg agacgttcta gcgttgttgg tcgtcctcgt cctgggtggg tcgggcgtcg
     3301 ccaccgccgc tgtcttcgcg ggcgccggtt cgccctcagg tggatctcct ggtcgctcag
     3361 acaaggccga ggccatcgag aaggactccg gcatccacgg cgggccgatc gagcggttcc
     3421 acctcgccgg gtcgtgcgat ctgacggccg tgggctcgct cccaggtaac tggacgcatg
     3481 gcgactacgt gagcgcggtc gctgccgggg gtgaggccgc cgagatccgc gaggcggcgc
     3541 ggagcgactg cggcaagccg atggtcgcgg tcggacacgg cggcgggcca gcggagcacg
     3601 cgctcgagca catggcggcc ggccaggcgc atacgggcaa gggcgacgac tcgggttcgg
     3661 acaccgtcgg cagttagccg ctgctgccca ggcccaatgg ctgcgcagac gcgccatcag
     3721 ccagaccgcc cctcgttcca ggtcccggga actcgagcgc gatcgaggtc gacagtcgtg
     3781 atggggtcag cggtgacggc tacgggttcg ccgatcccga gaggccgagg gcgccgccgc
     3841 cggggttcga gcgctcgccg ctgcggcgtc gttcgccgac ggcgctgtcg cgcggcgcgc
     3901 gccaagccgc taccgacgat ctgctgaggt gttcctgcgc agtcgtctgg gtcgtgacgt
     3961 gcattcggcc gacctcgtcg acatatgcct ccaagccgaa ccggatcgcc tcgctgtacc
     4021 tcgcccacgc ttgctcgcgc gtctcatacg tgaagcgtcg gacgtacctc gcgccgttgc
     4081 ggctctcgag ggggtcgatg acctccatca cgtacacgat ctgcatctcg tcctcgtcag
     4141 ccgcgtgtcg ggatccggct ggacacagcg cctcccgagt gatgtccaca ggcggcctcg
     4201 ttgccggccc gcgacgtcag tatcgacatg gtcggagttg cggtgaatgc gtcgaaagcc
     4261 cgaatctgcc cgtgggtttg cccgatgaag gaggtccccc aagaggtgtg ggccctctcc
     4321 ggagagggcc gcgtcgacca tcatcccccg tcgtgtccga tatggtgacc gaccctggaa
     4381 ccgcatggct ctggatgccg acgtttttcg ggatcgagct gggacgacaa gccggggcaa
     4441 tcctcgccgg gtggatctcc caaccgtgat ggatcgtcgc aggagtggag ctgctggccg
     4501 ctgcatctgc caccaggagt tgtgatggga gtcaacgact cgtgattcca catggtcctc
     4561 cacctctctt atgcatgtcc ggcgagctca gttgagtcgg gggtgggatg aggacggtcc
     4621 cgatgacgcg gccctcaccg tcgacgacct gcatcccaca atcgatgcag ccgtaaggaa
     4681 cctcatcgac gagcgcctcg agcggttcat actcgagacg aggcggcgag cgatgtcgag
     4741 gacgagaaga cggcccgttc gagctcgcat cgagggtcgt cgtgacgaac agcaacaaag
     4801 ccgactcaat aaccgggggt gctccctgcg ccgctcgcag atcatggctt cggtctcggc
     4861 atcgatctgg tgcgaacacg acgtcgggcg gtgggagtgg tcggcgagcg aggtgagtcc
     4921 gccttgctcg tagcgggcga tccagcgtgg acggtctggc gcgagacgcc cactcgccgg
     4981 gcgacctcgt ccacccgcca gccgtcttgc accaccgcga gcacggcttg ataccgctgt
     5041 tccatgacgc tgagctccac cagcacggcc caccccccga gtcgagatga gcggtgtcaa
     5101 gcatcagccg gagccgccgt taaggatcag ccggagcccg tgtcgtggat cagccggaac
     5161 tccgtcaagg atcaggtgac gccggaacgt ccagcatccc ccgaaacacg acatgagctg
     5221 gctccggggg tgggactcga acccacaacc tacggattaa cagtccgccg ctctgccaat
     5281 tgagctaccc cggatggagc cgaggcagtc taccaacggc ctcggattgg actaggctca
     5341 ggggatgcgg ttcaagac
//
LOCUS       DASUYD010000058         2757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_689490, whole genome shotgun sequence.
ACCESSION   DASUYD010000058 DASUYD010000000
VERSION     DASUYD010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_689490"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF195_03165"
     CDS             complement(<1..293)
                     /locus_tag="VF195_03165"
                     /inference="COORDINATES: protein motif:HMM:NF012651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6579855.1"
                     /translation="MGADMVTVNVVVLAGTVAADPVQRRMPSGDEVTELRLSVPEAGR
                     RLLPLPVAAWHATVGARTVDGIAKGDAVLVHGQLVRRFYRSGAGARSLTEVVAT"
     gene            363..1349
                     /locus_tag="VF195_03170"
     CDS             363..1349
                     /locus_tag="VF195_03170"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6579856.1"
                     /translation="MERLPPIVFPVADGITAIDTFMGGRARYTAAYLLEATETALVET
                     GPGTSVEPVTEALHSLGVAPDGLAHIVLTHIHLDHAGGVGRLAERFPRATVWVHQRGA
                     PHLVDPARLVASTARVWGESEMRELFGPTEPVASGRVRPLADTDTVALGDRDLFVLET
                     SGHASHHVALVDSRTGSVFTGDALGIHVPDLPVLRPATPPPEFDLERYVDSIERIRRA
                     ARSVLLFAHFGPLDEVDATCDLAIRRVRDWAGVVQRGVREGNDPDELVARLETAALAD
                     IETGAEAALDLEKLEDRLRLLSSIRMNAQGLARYWEKRRDRGAQEYDPTDAS"
     gene            complement(1342..2031)
                     /locus_tag="VF195_03175"
     CDS             complement(1342..2031)
                     /locus_tag="VF195_03175"
                     /inference="COORDINATES: protein motif:HMM:NF024100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX6579857.1"
                     /translation="MSGAFHQKGRGGPRAPAPNTALPRPIQDEVRRTAPPGKDREAMS
                     RLGRAIELLDRDDPKGAAAEAEKAKALAPRSGAVREVLGLAMYGLGRWQEALTELKAY
                     KRISGRTDQNHLIADSLRGLGRPKEAVPLAEEVLRVKGVPNEAKAEAVIVAASALADQ
                     GRFAEALAFLSRARTRDDRSEGYTLRLWYVKGDILARAGRKQEAAEEFRRIMQHDPSA
                     YDAAERLAEVS"
     gene            complement(2091..2207)
                     /gene="rrf"
                     /locus_tag="VF195_03180"
     rRNA            complement(2091..2207)
                     /gene="rrf"
                     /locus_tag="VF195_03180"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(2299..>2757)
                     /locus_tag="VF195_03185"
     rRNA            complement(2299..>2757)
                     /locus_tag="VF195_03185"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          402 a         1024 c          892 g          439 t
ORIGIN      
        1 gtggccacca cctccgtcag gctccgcgct cccgcaccgc tccggtagaa ccgtcggacg
       61 agctgcccgt gcacgagcac ggcgtctccc ttggcgatcc cgtcgacggt gcgcgcaccg
      121 accgtggcgt gccacgccgc gacggggagg gggaggaggc gtcgtcccgc ctccggaacg
      181 gacagtcgga gctccgtgac ctcgtctccc gacggcatcc gccgctgcac cggatccgcg
      241 gcgaccgtgc ccgcgagcac gacgacgttc accgtcacca tgtctgcccc cacttcgtgc
      301 ctccttccgg gggaaccgcg acggtatccc ccgccaccga cacggcctgc gacactgatc
      361 ggatggagcg tctcccgccg atcgtgttcc cggtcgccga cgggatcacc gcgatcgaca
      421 cgttcatggg gggccgagcg cggtacacgg ccgcctacct cctcgaggcc acggaaacgg
      481 cgttggtcga aacgggaccg ggcacgtccg tcgagccggt caccgaggcg ctccactcgt
      541 tgggcgtggc cccggacggg ctcgcgcaca tcgtgctcac gcacatccac ctcgatcacg
      601 cgggaggggt cggccggctc gcggagcgct tcccgcgcgc cacggtctgg gtgcaccagc
      661 gtggcgcacc ccatctcgtc gatccggctc gcctggtggc gagcacggcg cgggtctggg
      721 gggagtcaga gatgcgcgag ctcttcggcc cgaccgagcc cgtcgcgagc ggtcgcgttc
      781 gccctctggc ggacacggac acggtcgcgc tcggcgaccg cgacctcttc gtcctggaga
      841 catccgggca cgcgtcgcat cacgtggcgc tcgtcgactc gcgcaccggg tcggtcttca
      901 cgggcgatgc cctcgggatc cacgttcccg accttcccgt gctccggccg gccacaccac
      961 ctccggagtt cgacctcgag cggtacgtgg actcgatcga gcggatccgt cgggccgccc
     1021 gctcggtcct cctgttcgcg cacttcgggc ccctcgacga ggtggacgcg acgtgcgacc
     1081 tcgcgatccg ccgggtgcgc gactgggcgg gcgtcgtgca gcggggcgtg cgggagggga
     1141 acgatcccga cgagctggtc gctcgcctcg aaaccgccgc cctggcagac atcgagacag
     1201 gagccgaggc ggccctcgac cttgagaagc tcgaggatcg gctccgtctg ctctccagca
     1261 tccggatgaa cgcgcagggc ctggctcgct actgggagaa gcgccgcgac cgaggagcgc
     1321 aggagtacga tccgacggac gctagctgac ctcggccagg cgctccgccg cgtcgtacgc
     1381 gctcgggtcg tgctgcatga tccgacggaa ctcctccgcg gcctcctgct tcctgccggc
     1441 ccgcgcgagg atgtcgccct tcacgtacca cagacgcagc gtgtacccct cggatcggtc
     1501 gtcccgggtc ctcgcccgac tcaggaaggc gagcgcctcg gcgaaccggc cctgatcggc
     1561 cagggccgac gccgcgacga tcacggcctc ggccttcgcc tcgttgggga cgcctttgac
     1621 ccggaggacc tcctccgcga ggggcaccgc ctccttcggc cggccgagcc ctcggagact
     1681 gtcggcgatc agatggttct ggtcggtacg gccgctgatc cgcttgtacg ccttcagctc
     1741 cgtgagcgcc tcctgccacc tcccgagtcc gtacatcgcg agcccgagta cctctcgcac
     1801 cgcgccggag cggggcgcga gggctttcgc tttctcggcc tcggccgccg cccccttcgg
     1861 atcatcgcga tcgagcagct cgatcgcccg tcccaagcga gacatcgcct cgcggtcctt
     1921 gcccggcggc gcggtccggc ggacctcgtc ctggatcggt cgcgggaggg cggtgttcgg
     1981 cgcgggggcc cgaggccccc ctcgcccctt ctgatggaac gcgccggaca tgccgcaagc
     2041 ctacggaaac gcgaacgggc cgccctcgcg ggcggcccgc tcgtacgaag tctcggcagc
     2101 gacctactct cccactccgt ctccagagca gtaccatcgg cgctggaggg cttaacttcc
     2161 gggttcgaga tgggaccggg tgttccccct ccgccatggc caccgaaaca ccttctccgg
     2221 atcacgacgt gccgatcgcg gaaccatggc gaggatgcgc cccgagcaga gctcggaaca
     2281 cgatagcgag cgcgagcaag agtcaagtcc tcggcctatt agtacgggtc agctccacac
     2341 gttgccgtgc ttccacttcc cgcctatcaa cctcgtggtc tgcgaggggc cttacctggt
     2401 cgagccagtg ggagatctca tctcgaggcg ggcttcgcac ttagatgctt tcagcgctta
     2461 tcccttccga acgtggccaa ccagccatgc cgctggcgcg acaactggca caccagaggt
     2521 tcgtccaccc cggtcctctc gtactagggg tagctcctct caaatctcct gcgcccacag
     2581 cggataggga ccgaactgtc tcgcgacgtt ctaaacccag ctcgcgtgcc tctttaacgg
     2641 gcgaacagcc cgacccttgg gacctgctcc agccccagga tgagacgagc cgacatcgag
     2701 gtgccaaacc ctgccgtcga tatggactct tgggcaggat cagcctgtta tccccgg
//
LOCUS       DASUYD010000059         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_249627, whole genome shotgun sequence.
ACCESSION   DASUYD010000059 DASUYD010000000
VERSION     DASUYD010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249627"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF195_03190"
     CDS             <1..332
                     /locus_tag="VF195_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579858.1"
                     /translation="IAFVVPESALLQLAVVPRFLVAVAIAFGPVFCANLVFAERFEMV
                     ERPTVAFGANLLGAMIGGTLEYASLVTGYRALLIVAALLYGLAFLSGRRYLVGRSTVG
                     TRVAVRV"
     gene            complement(800..994)
                     /locus_tag="VF195_03195"
     CDS             complement(800..994)
                     /locus_tag="VF195_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579859.1"
                     /translation="MERSHRIDQEEFYRMLEGVVIDDTELFNDRLQEWESFYNFNRPH
                     GGLGGQTPYERLREKTRAQA"
     gene            complement(1110..2513)
                     /locus_tag="VF195_03200"
     CDS             complement(1110..2513)
                     /locus_tag="VF195_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6579860.1"
                     /translation="MQLRLGIDIACRAPHQASLADERGEFVFTGRRFRTSVEDLEHLW
                     TMLPEGTESGDVTVIMEPTRNAWVPLAAWFRRRGARVVLVPPERSADLRAYYAKHTKT
                     DRLDSRMLARLPLLHPEGLHEEHGLGPGDRLRRATRLHQTLTRRRSQSLARLDAMLEI
                     LGPSWLGVFGADLSNKTPLRFLAAGYACPLTVKRLGKARVARFLYRHSRGRWGEEEAE
                     ALLGAAAETLALWADELEFAELADDIAIEARLALHLGEELKELDERIAIFLAEADPTG
                     ILTSVPGIGRVLGGQILGRLGDPNRFRTLAGVRSFSGLVPSLDASGVSGRHGGPTKRG
                     DACLREAVFMAADQARRCDPSLAARYNRLMVEAGKHHTSALCHVAAVLLTRIVACWRA
                     GEQYVIRDVDGTVVDLVEGRRIVAERYTVPADLRAVRRAGGRVEDRRGTDRRSKGSLR
                     APSTGPSLANATALAGA"
     gene            complement(2670..3428)
                     /locus_tag="VF195_03205"
     CDS             complement(2670..3428)
                     /locus_tag="VF195_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020114018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579861.1"
                     /translation="MTERELSRAAARRLAIIRHAEEVTGNVALTCRYYGISRTLFYTW
                     RRRYEELGIDGLRPRSRRPLTSPNATSGEVIGKIVHLRTTYHFGPAKISMYLKRYHDL
                     GVSTSGVWRILRRLEMNRLPASQRYRRHVDRWKRYEKPQPGHRVQIDVKFVAPLRGSR
                     KKYYQFTAIDDCTRIRVLRIYDRLNQTTAIRFVDYVLEKLPFAVEVIQTDIQPESAPQ
                     VLGSLGRRHEDREDRRPSTVVDDRSERTARLLAC"
BASE COUNT          586 a         1235 c         1197 g          605 t
ORIGIN      
        1 ccatcgcctt cgtcgttccc gagagcgccc tgctgcaact cgccgtggtt ccgaggttcc
       61 tcgtggcggt ggccatcgcg ttcggacccg tgttctgcgc caacctggtg ttcgccgagc
      121 ggttcgagat ggtcgaacgg cccacggtgg cgttcggcgc gaacctgttg ggcgcgatga
      181 tcggcgggac gctcgagtac gcctcgctcg tcaccgggta tcgagcactc ctcatcgtcg
      241 cggcgcttct gtacggactg gcgttcctct cgggacgtcg gtacctggtc gggcgctcaa
      301 ccgtgggcac gcgggtcgcc gtgagagtct aagcagtcct gccctcaggc ctccgagctc
      361 cgcgaacacc gagagcgttg ctagctgacc acgccgcttg acgtcccgga atcgcagccc
      421 aagctcgcgg aggagcgggc aagctcttac aggccttcta cgcgaacgct atcccgctcg
      481 atctgctcaa ggcggagcag gaccgcatcg gcgcagcaga gcaggcagcc aaggcagagc
      541 tcgagacgag gcagggcgac ctagacggtt ggcaggacat tctccgaacg gccatccgac
      601 tcgccggcaa ctgccacgag gcgtaccaga agcgcggccg tctgttcggc gacgcttcaa
      661 cgacgcggtg ctccaggccg tctacatcaa ggaccggatc ggtcgaacgg agttctcgga
      721 ggtatttcgc atctcttctc tcgcccgagt tcgaataagc caagagtgga cccaagggtg
      781 tgcagctgac ggtgtccgtt cacgcttggg ccctcgtctt ctcgcgaagc ctctcatacg
      841 gcgtctggcc accgagccct ccatggggcc ggttgaagtt gtagaagctc tcccactcct
      901 ggagccgatc gttgaacagc tcggtgtcgt cgatcacgac cccctcgagc atccggtaga
      961 actcctcctg gtcgatccgg tgcgaccgct ccaccttccc gttcagccga ggtgtggccg
     1021 gcttgatgta gacgtggccg atcccggcat caaggacgtg gtagtggaac tgggacccga
     1081 actccggccc gttgagttcc caaccgatgt caagcccctg cgagggccgt agcgttggcc
     1141 agggacgggc cggtcgatgg ggcgcgtagc gaccccttgc ttcgccggtc cgttcccctt
     1201 cggtcctcga ctcggccgcc cgcgcggcgg accgctcgca ggtcggcggg gaccgtgtag
     1261 cgctcggcga cgatgcgcct gccttcgacg aggtcaacca cggtgccgtc gacgtcgcgg
     1321 atgacgtact gctcacccgc ccgccaacag gcgacgatcc gggtgagcag gaccgcggcg
     1381 acgtgacaca gcgctgaggt gtggtgcttg cctgcctcga ccatcaaccg gttgtagcga
     1441 gccgcgaggc tcggatcgca tcgccgggct tggtcggcgg ccatgaacac cgcctcccga
     1501 aggcatgcgt cacctcgctt cgtgggacct ccatgccgcc cgctcacccc cgaggcatcg
     1561 agggaaggaa cgagacccga gaacgaccgg accccggcga gggtccggaa gcggttgggg
     1621 tcaccgaggc gtccgaggat ctggccaccg agcacccggc cgatgcccgg caccgaagtg
     1681 aggatcccgg tcgggtcggc ttcggcgagg aagatcgcga tccgctcgtc gagctccttc
     1741 agctcctcac cgaggtgcag cgccaaccgg gcctcgatcg cgatgtcgtc ggcgagctct
     1801 gcgaactcga gttcgtcggc ccaaagggca agggtctcgg ccgccgcccc gagcagcgcc
     1861 tcggcctcct cctcgcccca cctcccgcgc gagtggcggt agaggaaccg ggcgacccga
     1921 gccttgccga gccgcttgac ggtgagcggg cacgcgtagc cggccgcgag gaaccgcagc
     1981 ggtgtcttgt tcgacagatc ggccccgaac accccgagcc acgaagggcc caagatctcg
     2041 agcatcgcgt cgaggcgggc gagactctgg ctccggcgcc tcgtgagcgt ctggtgaagc
     2101 ctcgtcgcgc gacggagccg atcaccggga ccgagcccat gctcctcatg cagaccctcg
     2161 gggtgcagca gcggcaggcg cgcgagcatc cgggagtcca accggtcggt cttcgtgtgc
     2221 ttggcgtagt aggcgcgcag gtccgccgat cgctccggtg ggacgagtac gacgcgggcc
     2281 ccgcgtcgac ggaaccatgc ggccagcggc acccaggcgt tgcgggtcgg ctccatgatc
     2341 accgtgacgt ctccggactc cgttccctcc ggcagcatcg tccaaaggtg ctcgaggtcc
     2401 tcgacgctcg ttcggaagcg ccgacccgtg aacacgaact cgccccgctc atccgcgagg
     2461 ctcgcctgat ggggagcccg gcaggcgatg tcgatcccca gtcgtagttg catgctcctc
     2521 ccttctcgtt cgcacgtccc ggcttcgacc gccgaactgg gaggcgctgc accggacatt
     2581 cgctaacagg gatccggacc acccacggcc cggtcaccgg gttcccaaca gggcgcacca
     2641 ccccggcggt ggccccgccc acctcgtgat caacaggcaa ggagtcgcgc ggtgcgctcc
     2701 gatcggtcat caacgacggt ggacgggcgc cggtcctccc gatcctcatg ccgccggcca
     2761 agcgatccaa ggacctgagg agccgactca ggttgaatgt cggtctggat cacctcgacg
     2821 gcgaacggga gcttctcgag cacgtagtcg acgaaccgga tcgcggtcgt ctggttgagc
     2881 cgatcgtaga tccgaaggac acggatccgg gtgcagtcgt caatcgctgt gaactggtag
     2941 tacttcttgc gcgagccccg aagcggcgcc acgaacttga cgtcgatctg cacccgatgc
     3001 cccggctgag gcttctcgta gcgcttccag cgatcgacgt ggcgccggta gcgctgggac
     3061 gccggcaggc ggttcatctc caggcgcctc aggatccgcc acacccccga cgtgctcacc
     3121 cccaggtcgt ggtagcgctt caagtacatg gagatcttcg ccggtccgaa gtgataggtc
     3181 gtgcgaaggt ggacgatctt gccgatcacc tcaccgctcg tcgcgttggg gctggtgagc
     3241 ggccgccggg agcgcggtcg cagcccgtcg atcccgagct cctcgtagcg gcgacgccac
     3301 gtgtagaaga gcgtccgcga gatcccgtag taccggcacg tcaacgcgac gttgcccgtc
     3361 acctcctcgg catggcggat gatcgccagc cgcctggcag ccgcccgtga aagctcccgc
     3421 tcggtcatcc ggttccccct tcctcagaga gaacccgatg tgtacacgat gtccgtcagt
     3481 tccagactgc ggagacggga gtgcgcgagt tcaccggata gtggacacct gtgtcggagc
     3541 atcgtggaca ccgatgacgg gggtctgcga tggatctggc tcggtacgtg gtggacgcgg
     3601 tcgtgctcga gggccggagc tat
//
LOCUS       DASUYD010000060         5961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1793344, whole genome shotgun sequence.
ACCESSION   DASUYD010000060 DASUYD010000000
VERSION     DASUYD010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1793344"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1372
                     /locus_tag="VF195_03210"
     CDS             <1..1372
                     /locus_tag="VF195_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579862.1"
                     /translation="LMREVATELWRSSAKDVRPEQERIVSLLSRDQAIRSLIASSDER
                     FQALSTRAGRVEDALGDLAETGRATREAIESSVASIREIADSPTLHGVEGVRNQLEQV
                     ERHIAETFKNLDERDRTLTDTILQQVREHGEMVARETSRVVEAMQGYVQGGAEAVGRL
                     AQRVEAHAESFASQDGDLTANVGATVREEVQAIGQQLELMSERMGIHSRDEVALKSAM
                     ERFIDARVRGLAELIRSDSQALRRMIEEKTTEQEVLLRETVEERMILVGEALVEKTAE
                     AAEIAIASSLGETVERMSASMGSIEGIDTMLAEQQQAFEERMRAHVDDRVAAIARMIR
                     SDNQALADRLGSAGPANDDAELLRQTLRSIKELHAGLANDVLSSVDRRFQSMSDQLHK
                     ESQSTAESMLTVAERLADKIDRVTVMVDEGYGNDVQIVVDRMSEAIQAMSGRTQRRDA
                     QGQLGA"
     gene            complement(1379..1801)
                     /locus_tag="VF195_03215"
     CDS             complement(1379..1801)
                     /locus_tag="VF195_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HEX6579863.1"
                     /translation="MPSPGQVEVGSELPTRSRIVHTEDVKAYAEAGGDANPLHQDEAV
                     ARAAGFPGIVAHGMFTMGHLAACIAEWAGGPDRVRRLTSQFRAPVFMGEEIVAGGRVK
                     SIDAERAVAVIECWVTLERDGRTEWPIRRGEAEVLLGE"
     gene            complement(1801..2187)
                     /locus_tag="VF195_03220"
     CDS             complement(1801..2187)
                     /locus_tag="VF195_03220"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6579864.1"
                     /translation="MDAAAVGKAYPEAPFTVDPGRVAAFRGVFSQVGGVPVTFATAAE
                     FTVIPEIVADPEVAIDFSRVLHGSQEYEFRRPLREGETLTIRSRIESIRSFGASSFLV
                     LATELVEPGGEIVCTARSTLIERAAG"
     gene            2310..3050
                     /locus_tag="VF195_03225"
     CDS             2310..3050
                     /locus_tag="VF195_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579865.1"
                     /translation="MAARARPKDRKASGTAEGDIRVVIADDHRSFGEALQIALDNEGD
                     LKVIEVVTDGESAIESAQVQHPDVLLMDVGMPGVDGLEATRRIHREASNTKVILLSGH
                     DDDVVLARAVEAGARGFLRKTQAVSELADAIRRANRGEPLHAVSEVELSLARFRMQRR
                     TDGELAQRVERLTPRELEILQRVAAGEASIDIANELGMSRHTLRTHIQNVLTKLGVHS
                     KTDAVVAAIRFGKVKTNEKLAQADVDAS"
     gene            complement(3098..3265)
                     /gene="rpmG"
                     /locus_tag="VF195_03230"
     CDS             complement(3098..3265)
                     /gene="rpmG"
                     /locus_tag="VF195_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEX6579866.1"
                     /translation="MARKEGNRPKITLACSDCKRRNYNTVKNRVNDRDRIELKKYCRW
                     CRSHTLHRETR"
     gene            complement(3268..4452)
                     /gene="tuf"
                     /locus_tag="VF195_03235"
     CDS             complement(3268..4452)
                     /gene="tuf"
                     /locus_tag="VF195_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6579867.1"
                     /translation="MGKKKFERTKPHVNIGTIGHIDHGKTTLTAAITKRQAEKGMADF
                     TPFDQIDKAPEERERGITIAIAHVEYETDNRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPSIIVALNKCDMVDDPELLDLVELEVRELLSS
                     YEFPGDNVPVIQISALKALEGDETWNNKIDELLAACDSYIPEPTRDTERPFLMPIEDV
                     FSITGRGTVVTGRVDRGTLTKMSEIEIVGIKDTRKTIATDLEMFRKLLDEVQAGDNVG
                     VLLRGIDKDDVERGQVLAKPGSITPHTEFEAQVYILSKEEGGRHTPFFDGYRPQFYFR
                     TTDVTGQAHLPEGVEMVMPGDNVEMKVDMIAPIAMEEGLRFAIREGGRTVGAGRVTKV
                     VK"
     gene            complement(4494..4565)
                     /locus_tag="VF195_03240"
     tRNA            complement(4494..4565)
                     /locus_tag="VF195_03240"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4531..4533),aa:Met,seq:cat)
     gene            complement(4646..5458)
                     /locus_tag="VF195_03245"
     CDS             complement(4646..5458)
                     /locus_tag="VF195_03245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX6579868.1"
                     /translation="MIPIRDINPTSRTAWVTLALILANVAVFLLWEPTFAGQAEQQEF
                     FFCNAQIPFELTHQTSLADGGAEAREAIQETFRTGGGDAAGLQEFLDQTCPDKGWLAS
                     LFVSMFLHGGWLHLAGNMLFLWIFGNNVEDRLGRIVFVAFYVLGGLAATGLQVALDPN
                     SAVPNVGASGAIAAILGSYLVMFPRARIHTLVIFVFITFLDLPATVVLVGWFVLQFFS
                     GVGEMGRELGGGGVAYWAHVGGFAFGLAVAWLFFRSRGRRDPYALPPPPPGY"
     gene            complement(5551..>5961)
                     /locus_tag="VF195_03250"
     CDS             complement(5551..>5961)
                     /locus_tag="VF195_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579869.1"
                     /translation="AHVHFGWYGPDGVAIDPMRRLIAWLREAGADLPALLSKAGLSEA
                     GTTAEMLGSAGPTLDMVRSLLEDRLAASSFTGSRNASGSGSHGPLDLTPLLFVALVAL
                     TRRGTTGSRRHRPIGAPTWPLGSEWEDRRGPLLS"
BASE COUNT          985 a         2006 c         2029 g          941 t
ORIGIN      
        1 cctgatgcgc gaagtggcca ccgagctctg gcgctcgtcg gcgaaggacg tccgccccga
       61 acaggagcgg atcgtcagcc tcctgtcgag ggatcaggcg atcaggagct tgatcgcgtc
      121 gagcgacgag cggttccagg cgctctccac ccgcgcggga cgcgtggagg atgcgctcgg
      181 tgacctggcc gagaccggtc gcgccacccg cgaggccatc gagtcctcgg tcgcctcgat
      241 ccgcgagatc gccgactctc ccaccctcca cggggtcgag ggcgtccgga accagctgga
      301 gcaggtcgag cggcacatcg ccgagacgtt caagaacctc gacgagcgcg accgcacgct
      361 caccgacacc atcctgcagc aggtccgaga gcacggcgag atggtggcgc gcgagacctc
      421 tcgcgtcgtg gaggcgatgc aggggtacgt gcagggtggc gccgaagcgg tggggcgtct
      481 cgcccagcgg gtcgaggcgc acgccgagtc cttcgcgagc caggacggcg acctgacggc
      541 gaacgtgggg gcgacggtcc gtgaggaggt ccaggcgatc ggccagcagc tcgagctgat
      601 gtccgagcgt atgggcatcc acagccgcga cgaggtcgcc ctgaagtccg cgatggagcg
      661 cttcatcgac gcccgggtgc gagggttggc ggagctgatc cggagtgact cccaagcgct
      721 tcggaggatg atcgaggaga agaccacgga gcaggaggtg ctgctccgtg agaccgtcga
      781 ggagcggatg atcctggtcg gcgaggccct ggtagagaag acggccgagg ccgcggagat
      841 cgcgatcgcg tcgtcgctcg gcgagaccgt ggagcgcatg agcgcgtcga tgggctcgat
      901 cgagggcatc gacacgatgc tcgccgagca gcagcaggcg ttcgaggagc ggatgcgcgc
      961 gcacgtggac gaccgcgtgg cggcgatcgc tcggatgatc cgttcggaca accaggcgct
     1021 cgcggaccgg ctcggctcgg ccggcccggc gaacgacgac gccgagctcc tgcggcagac
     1081 gctgcgctcg atcaaggagc tgcatgccgg cctcgcgaac gacgtcctca gctcggtgga
     1141 tcgccgcttc cagtcgatgt ccgaccagct gcacaaagag tcgcagtcga cggcggagtc
     1201 gatgctcacg gtcgccgagc ggttggccga caagatcgac cgggtcaccg tcatggtcga
     1261 cgagggctac ggcaacgacg tccagatcgt ggtcgatcgg atgagcgagg cgatccaggc
     1321 gatgtcggga cggacgcagc gccgggacgc gcagggccag ctgggagcct gagccgcctc
     1381 actcgcccag gagcacctcg gcttcgccgc gcctgatcgg ccattccgtc cggccgtcgc
     1441 gctcgagggt cacccagcac tcgatgacgg ccaccgctcg ctcggcgtcg atcgacttca
     1501 cacgcccccc cgccacgatc tcctcaccca tgaagacggg ggcgcggaac tgcgacgtca
     1561 gtcgccgcac ccggtcggga ccccccgccc attcggcgat gcaggcggcc aggtgcccca
     1621 tcgtgaacat cccgtgcgcg acgatccccg ggaaccccgc ggcgcgggca acggcctcgt
     1681 cctgatggag cgggttcgcg tctccgccgg cctccgcgta cgccttcacg tcctcggtgt
     1741 gcacgatgcg cgagcgtgtc ggaagctccg accccacctc gacctggccc ggcgacggca
     1801 tcagcctgcg gcccgttcga tcagcgtcga ccgcgccgta cagacgatct cgccgccggg
     1861 ttccacgagc tcggtggcga gtaccaggaa ggaactggct ccgaacgagc ggatcgactc
     1921 gatcctgctc ctgatcgtca gcgtctcccc ctcgcgcagc gggcgccgga actcgtactc
     1981 ctgactccca tggaggaccc gggagaagtc gatcgccacc tccggatcgg ccacgatctc
     2041 ggggatcacg gtgaactccg ccgcggtcgc gaacgtgacg gggacccctc ccacctggga
     2101 gaacactccg cggaacgcgg ccacacggcc gggatccacc gtgaagggtg cctccggata
     2161 cgccttcccc acggcggccg cgtccatcgg gggatgctaa tccaccgtcg gtcggacggc
     2221 gtccggcgtc cctccgacgg gggactccgg aaaatccggc gctgggggta cgtgattccg
     2281 tagatcaaca tgcctacgat gccgcggtaa tggctgcccg agccaggccg aaggaccgca
     2341 aggcatctgg gacggccgag ggggacatcc gcgtcgtcat cgccgacgac caccgctcct
     2401 tcggagaggc cctgcagatc gcgctggaca acgagggcga tctcaaggtg atcgaggtcg
     2461 tcaccgatgg agagtccgcg atcgagtccg cccaggtgca gcacccggat gtgctcctga
     2521 tggacgtggg gatgcccggt gtcgacggac tcgaagccac caggcgtatc caccgggagg
     2581 cctcgaacac gaaggtcatc ctcctgtccg gccacgacga cgacgtcgtg ctcgcgcgtg
     2641 ccgtcgaagc gggagcccgc gggttcctgc ggaagaccca ggccgtctcg gagctcgccg
     2701 acgcgatccg ccgtgcgaat cgtggtgagc cgctccatgc ggtgagcgag gtcgagctct
     2761 ccctggctcg gttccggatg cagcggcgga ccgacggcga gctcgcgcag cgggtggagc
     2821 gcctgacgcc tcgagagctc gagatcctcc agcgggtggc tgcgggcgag gcatcgatcg
     2881 acatcgcgaa cgagctcggg atgagccgtc acacactgcg gacgcacatc cagaacgtcc
     2941 tgaccaagct cggggtgcac tcgaagacgg acgccgtcgt cgcggcgatc cgcttcggca
     3001 aggtcaagac gaacgagaag ctggcgcagg cggacgtcga cgcttcctga tcagttcgtc
     3061 cgatctccgc tgtcgcttcg ttacttgagc gcgagcgtta gcgcgtctcg cggtgcaggg
     3121 tgtgggagcg gcaccaacgg cagtacttct tgagctcgat ccggtcccgg tcgttcacgc
     3181 ggttcttcac cgtgttgtag ttgcgcctct tgcagtccga gcaggccagc gtgatcttcg
     3241 ggcggttgcc ttccttccgg gccatcgcta cttcacaacc ttggtaaccc ggcccgcgcc
     3301 gacggtgcgg ccaccctcgc ggatcgcgaa gcgcaaaccc tcttccatcg cgatcggagc
     3361 gatcatgtcg accttcatct ccacgttgtc gccgggcatg accatctcca cgccctcggg
     3421 caggtgtgct tggccggtca cgtccgtggt ccggaagtag aactggggac ggtagccgtc
     3481 gaagaacgga gtgtgccggc ccccttcttc cttggacagg atgtagacct gcgcctcgaa
     3541 ctcggtgtgg ggtgtgatcg agcccggctt cgcaaggacc tgcccgcgct ccacgtcatc
     3601 cttgtcgatg ccgcggagca ggacacccac gttgtcgccg gcctggacct cgtccaggag
     3661 cttgcggaac atctcgaggt cggtggcgat cgtcttgcgg gtgtccttga taccgacgat
     3721 ctcgatctcg ctcatcttgg tgagcgtgcc ccgatcgacc cggcccgtga cgacggtgcc
     3781 gcggccggtg atcgagaaga cgtcctcgat cggcatcagg aacggcctct cggtgtcgcg
     3841 ggtcggttcg gggatgtagg agtcacacgc ggcgagcagt tcgtcgatct tgttattcca
     3901 ggtctcatca ccctcgagcg ccttcagagc ggagatctgt atcacgggga cgttgtcgcc
     3961 cgggaactcg taggacgaga gcagctcgcg cacctcgagc tcgacgagat cgaggagctc
     4021 agggtcgtcg accatgtcgc acttgttcag cgccacgatg atcgagggga ccccgacctg
     4081 gcgggcgagc agcacgtgct cacgggtctg cggcatgggg ccgtcggccg ccgagaccac
     4141 gaggatcgcg ccgtccatct gggcggcgcc ggtgatcatg ttcttcacgt agtcggcgtg
     4201 gccgggacag tcgacgtggg cgtagtggcg gttgtcggtc tcgtactcga cgtgggcgat
     4261 cgcgatcgtg atcccgcgct ctcgctcctc gggggccttg tcgatctgat cgaacggcgt
     4321 gaagtccgcc atgcccttct cggcttggcg cttcgtgatc gcggcggtca gcgtcgtctt
     4381 accgtggtcg atgtggccga tcgtgccgat attcacgtgc ggcttcgtgc gctcgaactt
     4441 cttcttgccc atgtctcgtg gtctcctcgg agggtctgct cggctcttcc tcgtagcggg
     4501 gggcgggatc gaaccgccga cacagcgatt atgagccgct tgctctacca ctgagctacc
     4561 ccgcccggcg gtcgcccagt ttacctgcga cggtgcgctc gcggcacgac cccgcagtcc
     4621 tactgccgcg gtcctcggtc agccgtcaat acccaggcgg tggtggcggc agggcgtacg
     4681 gatcccgccg cccgcgcgac cggaagaaga gccatgccac ggccaagccg aacgcgaagc
     4741 cgcccacgtg cgcccagtac gcgaccccgc cgcccccgag ctccctcccc atctcgccca
     4801 ctccactgaa gaactggagg acgaaccacc ccaccagcac gacggtggcg ggaaggtcga
     4861 ggaacgtgat gaacacgaag atcacgagcg tatggatccg cgccctcggg aacatcacga
     4921 gataggagcc gaggatcgcc gcgatcgcgc cggaggcgcc cacgttcgga acagccgagt
     4981 tcgggtcgag tgcgacctgg agcccggtcg cggccagccc tccgaggacg tagaaggcga
     5041 cgaacacgat ccgcccgagg cgatcctcga cgttgttccc gaagatccag aggaacagca
     5101 tgttccccgc gaggtgcagc cagcccccgt ggaggaacat cgagacgaac aacgatgcca
     5161 gccagccctt gtccgggcac gtctggtcga ggaactcctg gagccccgcc gcgtcccctc
     5221 cccccgttcg gaaggtttcc tggatcgcct cccgcgcctc cgccccgccg tcggcgagcg
     5281 acgtctggtg ggtgagctcg aacgggatct gcgcgttgca gaagaagaac tcctgctgtt
     5341 cggcctgacc ggcgaacgtc ggctcccaga ggaggaagac cgcgacgttg gcgaggatca
     5401 gggcgagcgt cacccatgcc gtcctgctcg tcgggttgat gtcgcggatc gggatcacga
     5461 ccgctcgacc ttatcctgca acgggcaccc gcgagagaga gccggagaca gggatcgagt
     5521 tcgatcagct cgagtccacg ctgcaccgcc tcaggaaagg agcggtcctc gacggtcctc
     5581 ccattcggac ccgagcggcc aggtcggagc tccgatcggg cggtgccgcc gagatcccgt
     5641 cgttccccta cgggtcaggg cgacgagcgc cacgaacagc agcggtgtca ggtcgagcgg
     5701 gccgtgcgat ccggatcccg atgcgttcct cgatccggtg aagctcgacg cggcgagccg
     5761 gtcttccagc aggctgcgga ccatatccaa cgtcggtccc gcggatccga gcatctcggc
     5821 agtcgtacct gcttcgctga gcccggcctt ggagagcagt gcagggaggt ccgctccggc
     5881 ttcccggagc cacgcgatca ggcgtcgcat cggatcgatc gccactccgt ccgggccgta
     5941 ccagccgaag tggacgtgcg c
//
LOCUS       DASUYD010000061        24174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_691249, whole genome shotgun sequence.
ACCESSION   DASUYD010000061 DASUYD010000000
VERSION     DASUYD010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24174
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691249"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..615
                     /locus_tag="VF195_03255"
     CDS             <1..615
                     /locus_tag="VF195_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HEX6579870.1"
                     /translation="RLIAFSSVGHMGFVMLGIATLTEIGIQAAVFGMVAHGVITGMLF
                     FCVGSIYDRYHTRQIAEIGGGMSQRMPKLAGVFAFVAIASLGLPGLAGFWGEVMALLS
                     AFRPNEALLDGQVGLFRVLMVAGGVGTILTAGYFLWMLQRVNMGAMPERWNEHAFGDI
                     HSVEWMSWTPLLVLILALGLFPRLIFGVSQEAVSGIMAVLGAGG"
     gene            623..2083
                     /locus_tag="VF195_03260"
     CDS             623..2083
                     /locus_tag="VF195_03260"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX6579871.1"
                     /translation="MTVDYHAILPELILAGTIMLVLVVDLFLPARLKWGSMPIGLIGV
                     LGSLVAVLTLLGDERATFGGAFVVDNFAVLFKVFFLVAAVIVLSVSRHYFDQPGYYQG
                     EYYFLLLTSFLGCLLMPSSRDLLMLFISLELVSAPGFLMAAFRKWTVIGVEGGLKFFL
                     IGVLSTAVMLFGMSLIYGLTGGKTRLDEVAGALGQLDPSQEAMALASILFVVVGFAFK
                     VSAFPFQFWAPDTYEGAPVPVAAFLAVASKAAGFAGLLQLMFVAFIGQHEFWVPIFAF
                     LAIATMTIGNLVALQQRQFVRLLAYSGIAQAGYILLPFALVTTDQAVNQQAFASATAY
                     ILIYGIMNLGAFAVAVALSKRSPRLLISDFAGVARVAPFLAVGMTLFMVSLAGVPPAA
                     GFWAKILIFGAAIGRGEVLGPLLAAIMVVNSVVSVFYYLAIARQMIFQPLEDTTPVRT
                     PVLLAGVVGLSMAALLFFFLLPGTFSRLADISTLVA"
     gene            2170..3057
                     /locus_tag="VF195_03265"
     CDS             2170..3057
                     /locus_tag="VF195_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015618958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEX6579872.1"
                     /translation="MAPGINRLKTWILIAALGGLFVLIGSFWGTNGMVIALIVALVFN
                     FSMYWFSDRIAIATTHSKPVTEQEMPDYYRVVRELANEQQMPMPRLYVSDMMQPNAFA
                     TGRNPEHAAVAVTRGILQLLDERELRGVLAHELSHVANRDILVSSIAAAIGMSITFLA
                     RFAFFFGGDDEGGGNIFALLLAWILAPIAALLIQMAVTRSREYQADESGAFLSRDPDA
                     LASALLKLEQASRKVPAPASVSPAEAHMFIVNPMNALQARGMSGVASLFRTHPPTEAR
                     VERLRAIASELRGERGPIG"
     gene            complement(3070..4452)
                     /locus_tag="VF195_03270"
     CDS             complement(3070..4452)
                     /locus_tag="VF195_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6579873.1"
                     /translation="MDLERLTTRAAEIAERESADLLTRTTASKALYERAVKSLPSGVA
                     SNFQVGDPYPVYLEKGKGSSVWDVDGTEYKDFHGGFGVNIVGHAHPKIVEALEKAARE
                     AIHFAVTTPTTVALAEAICERFELEQVRIVNSGTEATMDALRVARAATGRDKVVKMEG
                     SYHGHHDSLLFSVVPEADVLGLRHDVDHEGKAWYTTQPTSKGIPRALWDTTIVVPFND
                     AGAVEQALADNEGEVAAVILEPVMMNVGIIEPQAGFLQALRDTCEKHGTVLIYDEVKC
                     GGTIAYGGTMERYGVKPHLSAFAKAIGGGSTIGAFGGENRFMEWVTKGAAQQGTFNGN
                     PLSSAAGLTALTQVLTRDSYDHLGKLGSMLAEGCDRAIADAGIPAHTVDLGAKGCVSY
                     RGERLTNYRDFLETHTELYWASYPWMVNRGIFMTPGDEEQWTISVQHTEDDVQRYIDA
                     FGEFCQALVA"
     gene            complement(4537..5031)
                     /locus_tag="VF195_03275"
     CDS             complement(4537..5031)
                     /locus_tag="VF195_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEX6579874.1"
                     /translation="MAKDQFSFDIVSEVDLQEVRNAVDQAAREISQRFDFKGTGTSVS
                     LEKEKIEMAAGTEDRLKAAWQVLQEKAVKRQVPLKSLQPGHVEQAAKGTVRQTVDVIQ
                     GIPDEKAKQISKFVRSSVPKTQTQIQGSQVRVSAKKKDDLQSAIRAVKEEDFGLALQF
                     TNYR"
     gene            5129..5210
                     /locus_tag="VF195_03280"
     tRNA            5129..5210
                     /locus_tag="VF195_03280"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5163..5165,aa:Tyr,seq:gta)
     gene            5287..6444
                     /locus_tag="VF195_03285"
     CDS             5287..6444
                     /locus_tag="VF195_03285"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6579875.1"
                     /translation="MASDEEPRLHRYPAGVRESPYLEVAIAEGAVMAWPYNRMWLPAS
                     YGRDRRVEYEALLERVTLWDVGCERALELTGADAVAFADYLLTRDIAGQTVGRCRHAT
                     ACDEDGVIICEALVLRLSDERVWICHGPADFPLWARAVALHTEYEVEVHEAPVSPLAL
                     QGPRALDVMKALAPGVASMPRYAWVWTSIAGVEVLVSRTGWSGEFGYEVFAPDVEPAR
                     RVWQEIRRASDPFGVLVTPVIGERAWECGVTDIRCGDNLEINPFEVGLEHTVDLDKHA
                     RFIGQDALRRIADGTTPRRHAVAFTIDGEDVPELETFWPVMAPGDGERIGVVWQAGYS
                     YALERHAGDALMDQQTAMGTPLELESPSGPISAMVVSRPLVPEAATEPPRN"
     gene            7499..8158
                     /locus_tag="VF195_03290"
     CDS             7499..8158
                     /locus_tag="VF195_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019943122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6579876.1"
                     /translation="MASLVVGTFLTLDGVMQGPGGPDEDRSGGFAQGGWSVGYWDEAM
                     GQIITEWTAQADALLLGRTTYQIFAAHWPHVPDDDPVGAVLNRVPKYVASTTLKTVEW
                     NNSSVLEGDVPSAVAALEDARSGEIQVHGSWRLLQTLIGHDLVDEYRLWTFPVLLGSG
                     KRLFDTGTIPAGLELIDSTTSSTGVVIGRYRRAGEIRYGSFAVDERGGVEALWTQGEE
                     P"
     gene            8326..8973
                     /locus_tag="VF195_03295"
     CDS             8326..8973
                     /locus_tag="VF195_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6579877.1"
                     /translation="MSIVRVHNFSISLDGFGTGEPQSHDAPFGHAGERLHEWMFTTRY
                     WREMVGEPGGTRGLDDAFARLHNPGIGAEIMGAGKFGYPGWHEDREWNGWWGPNPPFH
                     TPVFVLTHHTRPPIEMEGGTTFHFLDVSPAEALETAREAAGGQDLRIGGGATVIRDLL
                     AAGLVDHMHTVVVPILLGRGVRLWDGLEGVEKDFQVEATSSPSGVTHVTFTRAGV"
     gene            complement(9380..11017)
                     /locus_tag="VF195_03300"
     CDS             complement(9380..11017)
                     /locus_tag="VF195_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6579878.1"
                     /translation="MAADRYDVIVVGGGHNGLVAAAYLAKMGAKVVVLESRHKTGGAA
                     DTSSPFPGHPDVKVTTLSYVMSLMPPSIVRDLRLEAFGYKVVPMAGSYAPQPGSGGIR
                     DDEGKSGRFRETLSGFSKTDADVFEDWSAWLARGAAFLGPLLMETPPDLGSVTPGDLV
                     DQVRFALRHRTGMSSRLAADITKLFTMSAADLLDEWFESSELKALIATGGIIGTWAGP
                     EEPGTAYVLMHHAVGDLGNGDLSQWGYPIGGMGAVADALRASAESFGAEIRVESRVER
                     ILVEASAARSVVTGAGEEYRAPLVVSACHPKITFLEQIAREELPGEFVRDIENWRTRS
                     GTVKINLALSHTPVFRDDPNGEDPEIAGSSIQVSPNIRYLERAFEEARDGKASTAPFS
                     ETCIPSYYDPTLAPEGMHVMSMFTQWVPHEWAQERNQDELEAYADRLVDIHDDFMPGF
                     KDAIVGRQVIGPWQMENEWGLIGGNIFHGELSPDQLFHMRPAPGYADYRTPIRGLYQA
                     SSATHAGGGVTGLPGHHVVREIVKDRKRERFRRRGRG"
     gene            complement(11387..12058)
                     /locus_tag="VF195_03305"
     CDS             complement(11387..12058)
                     /locus_tag="VF195_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEX6579879.1"
                     /translation="MLLLAGVASGAIYVVGDLISVLLYNGSRPYSFRDQWISELTAYG
                     SPVRPLMVTIMTISGLLGFAFAYGILRAAGERRSLRWTGLMLVLAGVVVFPLHPFFPM
                     SSRWMESGGSNDTMHATLTFIWGPIIFLAVALSAAAYRGWFRLFSISALVAMMGFGVA
                     SGIAIQGIEQNDTPWAGAFERVNAYVLMAWLVLLALMVIRHELIAGTEEKNLAETPRK
                     ELVTV"
     gene            12831..14015
                     /locus_tag="VF195_03310"
     CDS             12831..14015
                     /locus_tag="VF195_03310"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX6579880.1"
                     /translation="MSTQMRDSSLAGERTEFRPVGAKRFAYLDNLRVVMVAGVIAGHA
                     IAGYAGMNWTYADVAEGEMGELSGAVFGLLILPFAVFVMALFFLVAGLLTPGSLDRKG
                     PGRFAWDRLIRLGIPLAAFMFVLWPLTTYGLYRAAGLDWSLWDVYEDDPILDNGPLWF
                     VEALLIYSLVYAAWVGWRARLGRAPKAVARGPFRARHLVVLGAGIAIATFIIRLWFPF
                     DSLQLTNAHVWQWPECIGLFGLGIVSARRGWLQPVPERIRRGCGWTALLATVALIVAF
                     ASTQDPDRSVEAFGGGWRWEALVLATIGGAMSVAASIWVLAFAQRRWGRAGRTARAMG
                     RSAYAAFMLQGLPLIVAALSIRSFEMPLEVKALLVAAAGIAGSFALGWTLVTRTRLGQ
                     IM"
     gene            14727..16364
                     /locus_tag="VF195_03315"
     CDS             14727..16364
                     /locus_tag="VF195_03315"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX6579881.1"
                     /translation="MRLQDGDHFDLRIHPVRKRVDGADLRMLGYNGSIPGPTLHVDQG
                     SEITVEVTNDGDVDATVHWHGLRLENRYDGVPHDTQAPIPIGGTFTYKVQFPDAGFYW
                     YHPHIREDFGLEMGLYGTIIVEPSDPSYWPAVDRQLTLTLDDLLVEDGQIAPFHLSGP
                     NYTAMGRFGNVMLINGETEFAGQGAEGEVVRLYLVNTANTRIFNFAVRGARAKLVGGD
                     SGRYERETWVDEVLLAPSERAVLDVLFEAAGEVRLEHRTPDHVYDLGAFSVTGTSTGT
                     AGGSFAELRVDTELTREHQSIQEDLARPPDKVLAFVSSMPLLYGRDIQTSSYACPMHP
                     EVTGSKPGTCPKCGMQLVPVQAEAEEPSSYVCPMHPEVTDTKPGTCPKCGMQLVPWAA
                     QPPSEEDHGSTHTQHETDGLEWEDLMPEINRASDSSNMFWKLIDRESGAVNGAINWAF
                     TIGDRVKIRLVNEMKSDHPMHHPFHIHGAGRFLVLSRDAEPESNLVWKDTVLVRAGQT
                     VDILLDVSNPGLWMAHCHIAEHTQSGMMFSFDVARAR"
     gene            complement(16528..18651)
                     /locus_tag="VF195_03320"
     CDS             complement(16528..18651)
                     /locus_tag="VF195_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579882.1"
                     /translation="MDDRATIPAEGSVDQRDQTVRSQADARISGHRRRRPTGAAPPLP
                     KQIGSTGWIWLALLLAVVVTGCFWLRADTAPLDRIDAWITDAVISIRAGWLDAIARQA
                     HTVGSRVGFAALGLLLVLATVWFRRWRHLVIWFLSLGVAGALLQGLELVSLRPRPFGV
                     RQLASWEGYATPSIPIGAIAILTIGVAYMLVVPGRPRSWAKLAAAGAIAITGVLRIYL
                     GVDHLTDVAFGAIVGISIPLVAFRAFVPNDIFPVAYGAHGKSAHLDVTGRRGEAIRTA
                     LRDQLGFTVHDVKPVGLEGSGGSTPLKLCVTDEQDRERTIFAKLYAKSHVRADRWYKL
                     GRTMLYGRLEDETPFTTVRRFVEYEDYTLGTLGRFGFPTPAPLGIVEITPEREYLIAM
                     EFFDDAVEIGEATVDAHVIDEALAMIRLMWDVGLAHRDIKPANLMVQRGELRVIDVFF
                     VQVRPSPWRQAVDLGNMMLVLALRSDARTVYEAALRYFTPDELAEAFAATRGIASPTQ
                     LRQQMKQDGRDLLAEFRSMAPTRRLIGLQRWSVRRVVLIAVSLVVFLLAGFTGVALLF
                     PTRGTVTAPLCGTAQAMQLMAQAVPTATRLPCVSTLPYGWGVSTAETVRGEVTFGIGA
                     GNGSSEPVTVTLTGSCPATPVADTEQIPIDGGCVTYRSTIADPTVPSFGPNGGLAFTP
                     RTDLIAAVAADNDQVLCGALATPCP"
     gene            complement(18716..19189)
                     /locus_tag="VF195_03325"
     CDS             complement(18716..19189)
                     /locus_tag="VF195_03325"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HEX6579883.1"
                     /translation="MELQEWLAFLHILAAIVWVGASITQSAIMTRASRNPDRALVAGL
                     AHEIGWLGPFLIGPAAIVVIGAGIWITVIEEWVAFSDTWIWLSLVLVGVSMVLGMAYF
                     GPEGRRIERIADDRGPGDAEFRRRMDRLLMIGRLDLLLLAVVLWLMVFKPGAPAP"
     gene            complement(19385..20731)
                     /locus_tag="VF195_03330"
     CDS             complement(19385..20731)
                     /locus_tag="VF195_03330"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6579884.1"
                     /translation="MSTIGSSSTVPRIRPFLRFTEAGLEGDFQAQYFRDNLGYVRAAL
                     VLGIAAWAFFAFSSAPPIGQEGYLAIHLVGIGVTSLSLLLTFVPGYARWWQIAIVVLV
                     LVSAALAQGHRFVTRHPADWTGVVALMLVLAFAFALFRLQYRYAALGGILAIGCYSAM
                     RGLVQAPGDIGLIEPDVDLLAFATVGTAAAFALERFARLLFLRERDLDRERERGDMLL
                     RNVLPVGIIDRLKIREPGFDDDRIAERYAEATVLFADLVGFTERAARMDPDELIDTLD
                     EVFNRWDELADRFGLEKIKTIGDAYMAVAGVPDLRTDHVEAAADMALRIRDTLSQLRW
                     PSGDPISVRIGIATGPVIAGVIGHRKFAYDIWGDTVNAASRLESVAAPGSIQVSEAIY
                     DRLSGNYAFSGPYVVDLKGKGATQARSLLDHTRATNGAVPRPVLVGGGGPWNSMTG"
     gene            complement(20781..21005)
                     /locus_tag="VF195_03335"
     CDS             complement(20781..21005)
                     /locus_tag="VF195_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019896271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6579885.1"
                     /translation="MDGWDWFWGAIMMLLFWGVPVAVIVFAVRAFGARSQHDAGGAKD
                     PQTILESRFAKGEISEEEFEERKRVLASHV"
     gene            21694..22182
                     /locus_tag="VF195_03340"
     CDS             21694..22182
                     /locus_tag="VF195_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579886.1"
                     /translation="MARTGVSRESPARTHHGAATGPTTVHGDYVPRRSHFWRHFVEMV
                     IAMVVGMAVLGFPFRAILGQFGYTRDEAFDRFPEIVCLVMTFNMAVGMVAWMRFRGHP
                     WRITAEMTLAMCVATAVALAMFWLHIIAADPMIALMHVLMLPAMLAVMLRRREEYAHA
                     YR"
     gene            22169..22897
                     /locus_tag="VF195_03345"
     CDS             22169..22897
                     /locus_tag="VF195_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579887.1"
                     /translation="MRIGDRIHGRWTHDGVGDRPPDVPLRSESRAWAQLGFACGVLAM
                     VAYTILSAVPLSPQLQAVAVSIFGPGIAVASAGLYEVLRLHRSTVGLRIALVANIAAA
                     STVTMMLFGQIAFKRWLELEFPTGPVATSSSPAYQAANGLQLGFDVVWDLFFAIGTLL
                     FALAMWTHPRFGRGFAITGGVLAVLLLTLNLGTFPEPPEKAGLVDVGPLVGLWYLAAA
                     VRMRSSLRWVDRMASGSAPPVPKD"
     gene            22903..23520
                     /locus_tag="VF195_03350"
     CDS             22903..23520
                     /locus_tag="VF195_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6579888.1"
                     /translation="MATRQITITPMSPDRVEEAGRVLGAAFASSPLERAVRGPIDERQ
                     HRGLVIAYTATCRLSGQVRIALSEQRIVGVIRWVESPRCQLRLGEKLAMAPTAIGAFG
                     RNLPKALMWVSRWSKRDPSRPHVHLGPIGVTPELQGRGIGSEMLSLYCEEVDLAGKPG
                     YLETDKRENVRLYERFGFEVQNEAELLGVRNWFMWRAPTRQRSVT"
     gene            23533..23709
                     /locus_tag="VF195_03355"
     CDS             23533..23709
                     /locus_tag="VF195_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579889.1"
                     /translation="MKRGLIGAVMFLLVVALLRRVGRPLGERAMEKCQEMFERGMPER
                     RGLPSERTVAPARR"
     gene            23775..24005
                     /locus_tag="VF195_03360"
     CDS             23775..24005
                     /locus_tag="VF195_03360"
                     /inference="COORDINATES: protein motif:HMM:NF021378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6579890.1"
                     /translation="MLILWSGLAALIVFLVRASGGRPSQGEDRHQRSEAREILAERFA
                     RGEVSEEEFEQRTRVLRRGGRSSSSRGGGRQR"
BASE COUNT         3941 a         8018 c         8059 g         4156 t
ORIGIN      
        1 cggctgatcg cgttctcctc ggtcgggcac atgggcttcg tgatgctcgg catcgcgacc
       61 ctcaccgaga tcgggatcca ggcggcggtc ttcggcatgg tcgcccacgg cgtgatcacg
      121 gggatgctgt tcttctgcgt cggatcgatc tacgaccgtt atcacacccg gcagatcgcg
      181 gagatcgggg gagggatgtc gcagcggatg cccaagctcg cgggggtctt cgcattcgtg
      241 gcgatcgcgt ccctgggcct gccgggcctg gccgggttct ggggcgaggt gatggcgctg
      301 ctctcggcgt tccggccgaa cgaggcgctc ctcgacggtc aggtcggcct gttccgcgtg
      361 ctgatggtgg cgggcggggt ggggacgatc ctgaccgccg gctacttcct gtggatgttg
      421 cagcgcgtga acatgggcgc gatgccagag cgctggaacg agcacgcgtt cggagacatc
      481 cacagcgtcg agtggatgtc gtggacgccg ttgctggtcc tcatcctcgc gctcgggctc
      541 ttcccgcggt tgatcttcgg cgtgagccag gaggcagtga gcggcatcat ggcggtgctc
      601 ggggcggggg gctgaggacc tcgtgaccgt cgactaccac gcgatcctcc ccgagctgat
      661 cctcgcgggg accatcatgc tcgtgctggt cgtcgacctg ttcctgccgg cgcgcctgaa
      721 gtgggggtcg atgccgatcg ggctgatcgg cgtcctcggg tcgctcgtcg cggtgctgac
      781 gctcctcggc gacgaacgcg ccaccttcgg cggcgcgttc gtcgtcgaca acttcgccgt
      841 cctgttcaag gtcttcttcc tggtggccgc ggtcatcgtc ctctcggtct cgcgccacta
      901 cttcgaccag ccggggtact accagggcga gtactacttc ctgctgctca cgtcgttcct
      961 cggctgcctc ctgatgccgt cctcacgcga cctcctgatg ctcttcatct ctctcgagct
     1021 cgtgtcggcg cccggcttcc tgatggctgc gttccgcaag tggaccgtga tcggcgtcga
     1081 gggcgggctc aagttcttcc tgatcggcgt cctgtcgacg gccgtgatgc tgttcggcat
     1141 gagcctgatc tacgggctga ccggcgggaa gacgcgtctc gacgaggtcg ccggtgctct
     1201 ggggcagctc gacccgtccc aggaggcgat ggccctcgcg tcgatcctgt tcgtggtcgt
     1261 cggcttcgcg ttcaaggtct ccgccttccc gttccagttc tgggcgccgg atacgtacga
     1321 gggcgcgccc gtcccggtcg cggcgttcct ggcggtggcg tcgaaggccg ccggtttcgc
     1381 gggattgctg cagctgatgt tcgtggcgtt catcggccag cacgagttct gggtgccgat
     1441 cttcgcgttc ctcgcgatcg ccacgatgac gatcggcaac ctcgtggcgc tgcagcaacg
     1501 gcagttcgtc cgactgctcg cgtactcggg gatcgcgcag gcgggctaca tcctgctgcc
     1561 gttcgccctg gtcacgacgg atcaggccgt gaaccagcag gcgttcgcgt ccgcgacggc
     1621 ctacatcctg atctacggga tcatgaacct cggcgcgttc gcggtcgcgg tggcgctcag
     1681 caagcggagc ccccggctcc tgatctccga cttcgccggc gtcgcgcggg tcgccccgtt
     1741 cctggcggtg ggcatgacgc tgttcatggt gtccctcgcg ggggtgcctc cggcggcggg
     1801 gttctgggcg aagatcctga tcttcggcgc cgcgatcggg cgcggggagg tgctcgggcc
     1861 gttgctcgcg gcgatcatgg tcgtcaactc cgtcgtgagc gtcttctact acctggcgat
     1921 cgcgcgccag atgatcttcc agcccctgga ggacaccacc cccgttcgca cgccggtgtt
     1981 gctggccggc gtcgtcggcc tctcgatggc tgccctgctc ttcttcttcc tgctcccggg
     2041 cacgttctcg cggctcgccg acatctcgac gctcgttgcc tgaaccgtcc cgttggggtc
     2101 gaacgaacga gctgtgagag cattgaccca cggggcatat cggccctgaa cgaggagcgt
     2161 gaagaagaga tggcacccgg catcaatcga ttgaagacct ggatcctgat cgccgcgctc
     2221 ggcggcttgt tcgtcctgat cggctcgttc tggggcacga acgggatggt gatcgccttg
     2281 atcgtcgccc tggtcttcaa cttctcgatg tactggttct ccgaccggat cgcgatcgcg
     2341 accacgcact cgaagccggt caccgagcag gagatgccgg actactaccg ggtcgtccgc
     2401 gagctcgcga acgagcaaca gatgccgatg cctcggctct acgtctccga catgatgcag
     2461 ccgaacgcgt tcgctaccgg ccggaacccg gagcacgccg ccgtcgccgt cacccgcggc
     2521 atcctccagc tcctcgacga gcgtgagctc cgcggcgtgc tcgcccacga gctctcccac
     2581 gtcgcgaacc gcgacatcct cgtgtcgtcg atcgcggccg cgatcgggat gagcatcacg
     2641 ttcctggcca ggttcgcgtt cttcttcggc ggcgacgacg agggcggcgg caacatcttc
     2701 gccctcctgc tcgcctggat cctggcgccg atcgcggccc tcctgatcca gatggcggtg
     2761 acgcggtcgc gcgagtacca ggcggacgaa tccggcgcgt tcctctcacg ggatcccgat
     2821 gctctggcca gcgcgctgct caagctcgaa caggcctcgc ggaaggtgcc ggcgcctgcc
     2881 agcgtcagcc cggcggaggc gcacatgttc atcgtgaacc cgatgaacgc gctgcaggca
     2941 cgcgggatga gcggggtcgc cagcctgttc cggacccacc cgccgacgga ggcacgcgtg
     3001 gagcgcctgc gggccatcgc gagcgagctc cgcggcgagc gcggcccgat cggctgatcc
     3061 gaacgtcctc tacgcgacga gtgcctggca gaactcgccg aacgcgtcga tgtagcgctg
     3121 gacgtcgtcc tcggtgtgct ggacggagat cgtccactgc tcctcgtcgc ccggcgtcat
     3181 gaagatcccg cggttcacca tccacgggta ggacgcccag tacaactccg tgtgcgtttc
     3241 gaggaagtcg cgatagttgg tgagtcgctc tccacggtag gagacgcagc ccttcgcgcc
     3301 caggtccacg gtgtgcgcgg ggataccggc gtcggcgatc gcgcggtcgc agccctccgc
     3361 gagcatcgag ccgagcttcc cgaggtgatc gtaggagtcg cgcgtgagca cctgcgtgag
     3421 cgccgtgagg ccggccgccg acgagagagg gttgccgttg aacgtgccct gctgggcggc
     3481 gcccttcgtg acccactcca tgaagcggtt ctcgcccccg aaggcgccga tcgtggagcc
     3541 gccgccgatc gctttcgcga acgcggacag gtggggcttc acgccgtagc gctccatcgt
     3601 cccgccgtac gcgatcgtcc cgccgcattt cacctcgtcg tagatcagca ccgttccgtg
     3661 cttctcgcag gtgtcgcgga gcgcctggag gaagccggcc tggggctcga tgatgccgac
     3721 gttcatcatc acgggctcga ggatcaccgc ggcgacctcg ccctcgttgt ccgcgagggc
     3781 ctgctcgacg gcgccggcgt cgttgaacgg caccacgatc gtcgtgtccc acagcgcccg
     3841 cgggatgccc ttcgacgtgg gctgggtggt gtaccaagcc ttgccttcgt gatcgacgtc
     3901 gtgccggagg ccgagcacgt cggcctcggg gaccacggag aacagcaagg aatcgtggtg
     3961 gccgtggtac gagccctcca tcttcacgac cttgtcccgg cccgtcgcgg cgcgcgccac
     4021 gcgcagcgca tccatcgtcg cctcggtccc ggagttcacg atccggacct gctcgagttc
     4081 gaagcgctcg cagatcgcct ccgcgagggc gaccgtggtg ggcgtcgtga cggcgaagtg
     4141 gatcgcctcg cgggcggcct tctcgagcgc ctcgacgatc ttcgggtggg cgtggcccac
     4201 gatgttcacg ccgaagccgc cgtggaagtc cttgtattcc gtgccgtcca cgtcccagac
     4261 gctcgagccc ttgcccttct cgaggtacac ggggtaggga tcgcccacct ggaagttgga
     4321 ggcgacgccg ctcgggaggc tcttcacggc gcgttcgtac agcgccttcg acgccgtcgt
     4381 cctggtcagc agatcagcgc tctcgcgctc ggcgatctcc gccgctcggg tcgtcagccg
     4441 ttccagatcc atctccacct cctgagggtc aggggaatct acaggcgcgc cctggacccg
     4501 tgcaatacag catcgtgcgg ggggtcgccc gagggctcag cggtagttcg tgaactgcag
     4561 cgcgagaccg aagtcctcct ccttgacggc ccggatcgcc gactggaggt cgtccttctt
     4621 cttagcggac acgcggacct gggacccctg gatctgggtc tgcgtcttgg gaacgctgct
     4681 gcggacgaac ttggagatct gcttggcctt ctcgtcaggg atcccctgga tgacgtcgac
     4741 ggtctgccgg accgtcccct tggcggcctg ctcgacgtgt ccgggctgga gcgacttcag
     4801 ggggacctgt cgcttgacgg ccttctcctg gagcacctgc cacgcggcct tcagccggtc
     4861 ctcggtgcct gctgccatct cgatcttctc cttctcgagg gagaccgagg tgccggttcc
     4921 cttgaagtcg aaccgctgag agatctcgcg cgccgcctgg tccacggcgt tgcggacctc
     4981 ctgcagatcg acctccgaca cgatgtcgaa cgagaactga tccttcgcca tgggccaatc
     5041 ctagaaggag tgcgtggtct gcgtcccgga accggggcct tcccgagagg ggacatcgcc
     5101 ggtcgattgc tatcctcgac cgtgttccgg gaggatgccc gagtggccaa agggagcggt
     5161 ctgtaaaatc gccggcgtag cctacggagg ttcgaatcct cctcctccca ccgaccgaac
     5221 gaaccgatcc acccacccac cgaccgaccg atgaccgctt cacgccttcc cagctagggt
     5281 tcgacgatgg cctccgatga ggagccccgg cttcaccggt acccggccgg tgttcgcgaa
     5341 tcgccctatc tcgaggtcgc gatcgcggag ggcgccgtca tggcttggcc gtacaaccgg
     5401 atgtggctgc ccgcgagcta cggacgtgac cgccgcgtcg agtacgaggc gctcctcgaa
     5461 cgcgtcacgc tgtgggacgt cgggtgtgaa cgcgcgctcg aattgaccgg ggccgacgcc
     5521 gtcgcgttcg ccgactacct gctcacgcgg gacatcgccg gacagaccgt gggccggtgc
     5581 aggcacgcga cggcctgcga cgaggatggg gtgatcatct gcgaggccct cgtcctgcgg
     5641 ttgagcgacg agcgcgtatg gatctgtcac ggtccggctg acttcccgct gtgggctcga
     5701 gccgtcgcac tgcacacgga gtacgaggtc gaggtccacg aggcgccggt atcaccgctc
     5761 gcgctgcagg gaccgcgggc tctggatgtg atgaaggcgc tcgcgccggg cgttgcctcg
     5821 atgccacggt acgcatgggt ttggacgtcg atcgccggcg tggaggtgct cgtttcgcgc
     5881 accgggtgga gcggcgagtt cggatatgag gtgttcgctc ccgatgtgga gcctgcgagg
     5941 cgtgtgtggc aggaaatcag gcgtgcttcc gatccgttcg gagtgctggt gacgcccgtc
     6001 atcggggaac gggcgtggga gtgcggcgtg accgacatcc gctgcggtga caacctggag
     6061 atcaacccgt tcgaggtcgg cctcgagcac acggtcgatc tcgacaagca cgcccgattc
     6121 atcgggcagg acgcgcttcg acggatcgcg gatggcacga cgccgcggcg tcacgcggtt
     6181 gccttcacga tcgacgggga ggacgtgccc gagctcgaga cgttctggcc ggtcatggca
     6241 cccggcgacg gcgagcgcat cggcgtcgtg tggcaagcgg ggtactccta cgcgctggaa
     6301 cgccacgccg gagacgcgct gatggaccag cagacggcga tgggtacccc gctggagctg
     6361 gagtcgccgt cgggtccgat ctcggccatg gtcgtgtccc gtccgctcgt ccccgaggcg
     6421 gcaaccgaac ctccgcgcaa ctgaccgtta ggggtactgg cagcgtgatc atgatgccgc
     6481 cccgtgaaca cttgcagggc gagtgtgagt actaccactc ttaacctttg ggtactcctc
     6541 tggcaggggc ccgttcgtcc aggtcaagca tttcgagaac accccaccaa ccaattcact
     6601 caccaccacc cgccaactca ccagccagcc aaccaatcga caccgaccga tcgagggatg
     6661 ctctcaagac cagctcgaac catggtgcgg gtcgtagcgg ggttcgttgc actcgttggc
     6721 cacgaagtcg aagacgtagc tggcggcggt tgacgacggg ttcgcccttc acctcacaac
     6781 cgtcgacacc acccatcgct tgtccctctg accgtctatc gagacggttc gtcacgcgac
     6841 ggtttcaatc cggtggatcg cacaagcacg ttccgtcgaa gtcagggttc gggtcagggc
     6901 acgcgatcgc gatgtgggtg tagccgcagt cccgacacct ttcacacgtc gggcactgac
     6961 ggagaacgtc cacgtcgatc tccttcagct tcaccgggtc cataacttgg tccatgactt
     7021 ccacctcccg agaagcgtct tcacagacaa cggtggaagg caacgcccgg cgacgatagg
     7081 ggcggaaggc ccctcattcg gcaggaccca acgagcgcgg ctcgccggta gcgccagcga
     7141 cccgtatcgc caacggaaag cgtcgtgagc gcgtgcaggg ggaccgatga gtcgtggggg
     7201 cgggcgctcc cgggttctgg tcgaacggtt cgcgcgctgg agcagacgcg agtgggcaag
     7261 actcgcctgg gttcgcgctc tccccgaggt ttgcggaccc cgctaggcgt caacaccagg
     7321 tcggacttac atgcgcctga accagacgac gtaccctctg gtcgtcggcg cgggcgaacg
     7381 aaaggaagat cgaccaggga gacgatctgc tcggtccacc gacccatgag tctgcctctt
     7441 cagtcggtgt cgtagaacga tgatgcgcgc ctcgaagtac ggagaaggga gaacgcccat
     7501 ggcttcgctc gttgtcggaa cgttcctgac gctcgacggc gtcatgcagg gtcccggcgg
     7561 tcctgatgag gaccgaagcg gcgggttcgc tcagggcgga tggtctgtcg gctactggga
     7621 cgaggccatg gggcagatca tcacggagtg gaccgcacag gccgatgcgc ttctcctcgg
     7681 acggacgacc taccagatct tcgcggcgca ctggccgcac gtcccggacg acgatccggt
     7741 cggtgcggtg ttgaatcgcg tccctaagta cgtggcctct accacactga agaccgtgga
     7801 gtggaacaac tcgtcggtgc tcgaaggcga cgtgccgagc gccgtggccg cgctcgagga
     7861 cgcgcgaagc ggcgagatcc aggtccatgg cagctggcgg ctcttgcaga ccctgatcgg
     7921 gcacgacctc gttgacgagt atcgcctgtg gaccttccct gtcctgctcg ggagcggcaa
     7981 gcgactcttc gacacgggaa cgatccccgc tggcctcgag ctcatcgaca gcacgacctc
     8041 ttccacaggc gtggtcatcg ggcggtaccg ccgcgcgggc gagatccgat atggctcgtt
     8101 cgcggtcgac gaacgaggag gagtcgaggc actctggacc caaggggaag aaccttgatg
     8161 tgaccgcgcg ctccgatcgg catcgttcag atgattggac cgcggatagg gcacctgacc
     8221 tgctgtggcg ctcggtgtcg tccacagtgg cgagtttcgt agacgtctac gatgcgaact
     8281 caacctggtg gaaacgtgaa ccctgaatcc gaaggagcag caacgatgtc gatcgtccgc
     8341 gtccacaact tctccatctc gctcgacggt ttcgggaccg gcgaacctca gagccacgac
     8401 gcaccgttcg gccacgccgg cgaaagactg cacgaatgga tgttcacgac ccggtattgg
     8461 cgcgagatgg tcggcgagcc cggcggcacc cgcggcctcg acgacgcctt cgcgcggctg
     8521 cacaaccccg ggatcggcgc cgaaatcatg ggtgccggga agttcggcta cccgggatgg
     8581 cacgaggacc gggagtggaa tggctggtgg ggtcccaacc cgccgttcca cacaccggtc
     8641 tttgttctta cccatcacac gcgtccgccg atcgagatgg aaggcgggac gacgttccac
     8701 ttcctcgacg tgtcgcccgc cgaagcgctt gagaccgctc gggaggctgc gggcggacag
     8761 gacctgcgca tcggtggagg cgccaccgtg atccgcgacc tcctcgctgc ggggcttgtg
     8821 gatcacatgc acaccgtcgt cgttcccatc cttctcggcc gcggcgttcg gctctgggac
     8881 ggactggagg gcgtcgagaa ggactttcag gtcgaggcca cctcgtcgcc cagcggggtc
     8941 acacacgtga cgttcacccg cgcgggcgtc taagccgcca cctcctactc ggggaggtcc
     9001 ctgggcgcct acctggtgga tcccatcggc gccagagagc ttgccgaccg catcatgagc
     9061 gcttgcgcgg ctcgagtgag cggtcgaggg tgccgagtcg gccgttcggt tcgccctcga
     9121 cgcaaccgac ggcaagcgcc tgcgttcgcc gatcagccca ggcagcccct ctcgttgctc
     9181 tccgctcctg gatagcctct gacctgctgt gggaagtgcc ccgcttaacc tgtgggaggt
     9241 tctccagaag tgccggaaac cgcctggtca ggaggtttta agaacacccc accgtaccaa
     9301 ctactactca ccagctcggg gtcacgacag tcgatcgtcg gcgagtcgag tcgagcgacc
     9361 cgatgcccgt tgtccctctt caacctcgac ctcgcctgcg gaaccgctct cgcttgcgat
     9421 ccttgacgat ctcgcggacg acgtgatggc cgggaagccc ggtcactcca ccgccggcgt
     9481 gcgtggcgga ggacgcttga tagagcccgc ggatcggcgt gcggtagtcc gcgtagccag
     9541 gcgcgggtcg catgtggaac agctggtccg gcgacagctc accgtggaag atgttgccgc
     9601 cgatcagacc ccactcgttc tccatctgcc acggcccgat gacctggcgg cctacgatcg
     9661 cgtccttgaa gcccggcatg aagtcgtcgt ggatatcgac gagtcggtcg gcgtacgcct
     9721 cgagttcgtc ctggttgcgc tcctgcgccc actcatgagg cacccactgc gtgaacatcg
     9781 acatcacgtg catgccctct ggcgcgagcg tcgggtcgta gtagctcggg atgcaggtct
     9841 ccgagaaggg cgcggtggag gccttgccgt cccgcgcctc ctcgaaggcc ctctcgaggt
     9901 atcggatgtt gggggagacc tggatggagg agcccgcgat ctcgggatcc tcgccgttgg
     9961 ggtcgtcccg gaagacaggc gtgtgcgaga gcgccaggtt gatcttgacg gtcccgctcc
    10021 gcgtcctcca gttctcgatg tcgcggacga actcgccggg cagctcctca cgggcgatct
    10081 gctccaggaa cgtgatcttc gggtggcagg ccgagacgac cagcggtgct cggtactcct
    10141 cgcccgcccc ggtcaccacg ctgcgcgccg cgctggcctc gaccaggatg cgctcgaccc
    10201 gactctcgac ccggatctcg gcgccgaagg actccgcgct cgcccgcagc gcgtcggcca
    10261 cggctcccat cccgccgatg gggtaccccc actgcgagag atcgccgttg ccgaggtcgc
    10321 cgacggcatg atgcatcaag acgtaggccg ttccgggttc ctccggtccg gcccacgttc
    10381 cgatgatgcc gccggtcgcg atgagcgctt tgagttccga cgactcgaac cactcgtcga
    10441 gcagatccgc ggcgctcatg gtgaagagct tggtgatgtc ggcggcgagg cgggagctca
    10501 tgccggtccg atgccgaagc gcgaagcgca cctggtccac gagatcccct ggggttaccg
    10561 atccgagatc cggcggtgtc tccatcagga gcggaccgag gaacgcggca ccgcgtgcga
    10621 gccaggccga ccagtcctcg aagacgtccg cgtccgtctt cgagaacccg gacagggtct
    10681 cgcggaaccg gccactcttc ccctcgtcat cgcggatacc tcccgacccc ggttgggggg
    10741 cgtaggagcc cgccatcggc acgaccttgt acccgaaggc ttcgagacgc aggtcgcgga
    10801 cgatcgaggg cggcatgagg ctcatcacgt aggagagcgt cgtgaccttg acgtcggggt
    10861 gccccgggaa cggggaggac gtgtcggcgg cgccgcccgt cttgtgacgg gactcgagca
    10921 ccacgacctt cgcccccatc ttggcgagat acgcagcggc gaccagcccg ttgtggcccc
    10981 cgccgacgac gatcacgtcg taccgatccg cagccatcaa cggtccttcc tcgtgctcgg
    11041 gcgaaggctg ttcgtcgcca ccctaccggt catcgtggat cgtcgaaggg gcctcagacc
    11101 tgctgtggga ggtacccctc ttaacctgtg ggaggtccac cagaaacgcc cgaaaccgtg
    11161 cggtcagggg gttttgagaa caccccacca accagccaac cgaccaccga ccgaccgatc
    11221 aatcccccac cgaccaccga ccgccgacga ccatatcgac cgaccgacta actaaccgac
    11281 caaccaaaca cggagtagtg gcgggccggg gcaggatgcc ggctgagccg cgcgatctcc
    11341 ttcccgcgcc agccaagcgg gctctggacg gagattgcgc gtgtcgttag accgtcacca
    11401 gctccttgcg tggggtctct gccaagttct tctcttctgt ccctgcgatg agctcgtggc
    11461 ggatgaccat caaggccagc agaacaagcc atgccatgag gacgtaggcg ttgacgcgtt
    11521 cgaaggctcc cgcccacggc gtgtcgttct gctcgatccc ctggatggcg atcccggacg
    11581 cgaccccgaa ccccatcatc gccacgaggg ccgagattga gaacagccgg aaccagcctc
    11641 tgtatgcggc tgcggagagc gccaccgcta ggaagatgat cggcccccag atgaacgtca
    11701 gggtcgcgtg catcgtgtcg ttggaaccgc cggactccat ccaccgagac gacatcggga
    11761 agaacgggtg aagcgggaac acaacgactc cggccaggac cagcatcagg ccggtccagc
    11821 gcaggctcct tctctcaccc gccgccctca agatcccgta ggcgaaggcg aaccccagca
    11881 ggccagaaat ggtcatgatc gtcaccatca agggccggac tggcgaaccg taggccgtca
    11941 actcgctgat ccactggtcc ctgaagctgt acggtctgga tccgttgtag aggaggaccg
    12001 agatcagatc ccccacgacg tagatcgcac cggaagcgac tccggcgagc aggagcacct
    12061 ttgtcaccgt acggttcgac ccccttcgtg acatgacgtc accacacgac tcgacttcca
    12121 cttgatccct cattgcactc acctcttacg ccatcctgct gcgctactag gtgaccgatc
    12181 ggtaccgaaa ggcccccggt ccgaaggacc ctcggacaag cccgtacggc tgctcctctc
    12241 gaacacccgt gcggcttgcc caccgctcac cgatatcgag cccggcggtt caggtacttg
    12301 ggcgatcccc ctctcgccgg tgacactgtg gttgggatcg ccgatcggcc ggtccttcga
    12361 cgcggacttc cccgaggaac cgagtgatgc tgccgctcac cctccttcga ccactccgtg
    12421 atcgacggcg ctcccgccgc ccgcttcgcc acgacgatcc gccggctgct cgagtcggca
    12481 tctgttctcg cggcgccggg gggagccctc gaggtcgcga gtccagcgcc gaccatcggg
    12541 gtggatgacg agcggcgacg ggtcggctcc cggaactagc caacccgaga tcacacccgg
    12601 gcgttcgatc ggaccggcgc gcttacggca tcgtgggcct cgatcacgat actggcgttc
    12661 tagcccgctg cccgtccgcg agatggcggc gagatcccgg cggcatcgac acctcctcga
    12721 gcgagcagcc atgagatcac cgagttcgag ggcagtcggg gccggcccgg cccgccgata
    12781 ccgggccgaa ccgccctaca ggaagcacga ccagcttcca tgcttgccgt gtgagcacac
    12841 agatgcgcga ttcatcgctc gcaggggaaa ggaccgagtt ccgtcccgtc ggggcgaagc
    12901 gctttgccta cctcgacaac ctgagggtgg tcatggtcgc cggtgtgatc gcggggcacg
    12961 cgatcgctgg gtacgcgggc atgaactgga cgtacgccga cgtcgcggag ggtgagatgg
    13021 gggagctgtc cggggcggtg ttcgggcttc tgatcctgcc gttcgctgtg ttcgtgatgg
    13081 cactgttctt cctggtggcg ggcctgctca ccccgggttc gctcgaccgc aagggtcctg
    13141 gccgcttcgc ttgggaccgg ctgatccggc tcgggatccc gctcgcggca ttcatgttcg
    13201 tgctatggcc cctgacgacc tacgggctgt accgtgccgc gggcctcgat tggagcctgt
    13261 gggacgtgta cgaggacgac ccgatcctcg acaacggtcc gctgtggttc gttgaggccc
    13321 ttcttatcta ctcgctcgtc tatgcggcct gggtcggatg gcgagcgcgc ctgggaaggg
    13381 caccgaaggc ggtcgcgagg gggccgttcc gtgctcgcca cctggtggtg ctgggcgcgg
    13441 ggatcgccat cgctaccttc atcatccggc tgtggttccc gttcgactcg ctccagttga
    13501 ccaacgcgca cgtctggcaa tggcccgagt gcatcgggct gttcggcctg gggatcgtga
    13561 gcgcgcgccg cgggtggctc cagccggtcc ccgaaaggat ccggcgcggg tgcggctgga
    13621 cggcgctgct cgcgaccgtg gcgctgatcg tggcgttcgc gtccacgcag gatcccgatc
    13681 gatccgtgga ggcgttcggt ggaggatggc gatgggaggc gctcgtcctc gcgacgatag
    13741 gcggtgcgat gtcggttgcc gcgtcgatct gggtcctcgc gttcgcccag cggcgctggg
    13801 gtcgcgccgg ccgaaccgct cgagcgatgg gccgctccgc ctacgcagcc ttcatgctcc
    13861 aggggcttcc cctgatcgtg gctgcccttt ccatccgctc gttcgagatg ccgctcgagg
    13921 tcaaagccct gctcgtggcc gccgccggta tcgcggggtc gttcgccctc gggtggaccc
    13981 tggtcacgcg cacacgtctg ggccagatca tgtgaggccg agcgcgtggc tgatgcgcag
    14041 aggggtctgg agaggactca acgattcgcc gccgtgccgc ggccggaacc ggacggaagc
    14101 cgtccaccgc gctagctact caggacagag tgacctccgg cctcggagcc caggtttgct
    14161 cacgacggaa gacctgacca gggacgctgt gccggcaatc gcgcgcgcgg aggcgaatcg
    14221 tccgaatcac ccggatcact tgcgcggccc ctgggacctg ctgtgggtac tcaccctctt
    14281 agcgcgtggg cactcctgag caacgagtct gttcgtgcag gtcagcgagt tctgagaaca
    14341 ccccaccgac cgacgaccag ccaaccgacc caccaaccac cgacaacccg ctcaccaacc
    14401 aggcaaccaa catagcggcc gtgtcggcac cctcgaccga cctgtcaccc gctggcgtag
    14461 agtgacgttt cacggagtgg ctctcgtggc cccgacctca acttgcgggg caccctctca
    14521 cccgttcctc cgggccaggg ttcgctgagc tcctcctcga gtcgggacgg gaaatcatgg
    14581 ttacggaacc taacgatcat ccgcatcagc ttccagcgct cgatcacgcc tcggatgagg
    14641 cgccacactc tgagggacag caggatgcgc gcgatctctt ctcgacagac acggccgcgc
    14701 tgccggaagc tgaaacaccg cgccccgtgc gactccaaga cggtgatcac ttcgacctgc
    14761 gtatacatcc cgtgcgcaag cgtgtcgacg gggccgacct ccgcatgctc gggtacaacg
    14821 ggtcgatccc gggcccgaca ttacatgtcg accaagggtc agagatcacc gtcgaggtga
    14881 ccaacgatgg tgacgtcgat gccaccgtgc actggcacgg cctacggttg gagaataggt
    14941 acgacggcgt tcctcacgac acccaggcac cgatcccgat cggcgggacg ttcacctaca
    15001 aggttcagtt cccggatgcc ggcttctact ggtaccaccc acacatccgt gaagacttcg
    15061 gcctggagat gggcctctac ggaacgatca tcgtggagcc ttccgacccc tcgtactggc
    15121 ccgcggttga ccggcagctg accctcacct tggatgacct gctcgttgag gatgggcaga
    15181 tcgctccttt ccatctatct gggccgaact acaccgcgat ggggcggttc gggaacgtca
    15241 tgctgatcaa cggcgagacc gagttcgctg gtcagggggc cgaaggagaa gtcgtgcgct
    15301 tgtatctcgt caacacggcg aacacgcgga tcttcaactt cgcggttcgc ggcgcccgcg
    15361 cgaagctggt cggcggcgac agcggtcgat acgaacgcga aacctgggtc gacgaggtac
    15421 tgctcgcgcc ctcggagcga gccgttctgg atgtgttgtt cgaagctgcg ggcgaggtgc
    15481 gtctcgagca ccgcacgccg gatcacgtct atgacctcgg agcgttctcc gttacgggga
    15541 cctcgaccgg aacagcgggc ggctcgttcg cggagctccg cgtcgatacg gagctcaccc
    15601 gtgagcatca gtccatccag gaggatctgg cccgaccgcc agacaaggtg ctcgcattcg
    15661 tgtcttccat gccgctcctc tatgggagag acatccagac atcctcctac gcctgcccga
    15721 tgcacccgga ggtcacgggc tcgaagcccg ggacgtgccc gaagtgcggg atgcaactgg
    15781 tgccggtgca ggcggaggca gaggaaccat cttcctacgt ctgtcccatg caccccgagg
    15841 tcacggacac gaagcccggg acgtgcccga agtgcgggat gcaactggtg ccctgggctg
    15901 cgcagccccc ttccgaggag gaccatggct cgactcacac ccagcatgag acggacggtc
    15961 tcgaatggga agacctgatg ccggagatca accgagcgtc ggactccagc aacatgttct
    16021 ggaagctcat cgatcgagag agcggcgcgg tgaacggggc gatcaactgg gccttcacga
    16081 tcggagaccg cgtgaagatc cgactcgtca acgagatgaa gtcggaccat ccgatgcacc
    16141 atccgttcca catccacggg gctggtcgct tcctcgtctt gtccagggac gccgagccag
    16201 aatccaacct cgtgtggaag gacacggtcc tggtgcgcgc ggggcagacg gtggacatcc
    16261 tgctggacgt ctcgaacccc ggactttgga tggcgcactg ccatatcgcc gaacacacgc
    16321 agagcggaat gatgttcagc ttcgatgtgg cgcgagcgcg gtagccaaca tggccgaagg
    16381 catccaggcg acgaacggcg gcagtcgatc cacgcgcgac agttcgaacg gtccggagct
    16441 ggatgtgatc gtcgtagggg cagtcaggcg gggactagcc atggcggccc ttcaccccag
    16501 gctgtccgat ggcatccggg tccgtctcta ggggcacggc gtggcaagtg caccgcacaa
    16561 cacctggtcg ttgtcggcgg cgaccgctgc gatgaggtcg gtgcgaggcg tgaaggcgag
    16621 gccaccgttc ggcccgaacg agggcaccgt cgggtcggcg atcgtcgacc ggtacgtcac
    16681 gcagccccca tcgatgggga tctgctcggt gtccgcgacg ggggtcgccg ggcaggaccc
    16741 ggtgagggtc acggtcaccg gctcgctcga gccgtttccg gcgccgatcc cgaaggtcac
    16801 ctcgccccgg acggtctcgg cggtgctcac gccccacccg tagggcagcg tcgagacaca
    16861 cgggaggcgc gtggcggtcg gaaccgcctg cgccatcagt tgcatcgcct gcgcggttcc
    16921 gcacagcggc gctgtcacgg tgcctcgcgt ggggaagagg agcgccacgc ccgtgaaccc
    16981 cgcgagcagg aacacgacga gtgacacagc gatcagcacg acgcggcgga cgctccagcg
    17041 ctgcagaccg atcaggcggc gcgtcggcgc catcgaccgg aactcggcga gcaggtcgcg
    17101 tccgtcctgc ttcatctgct gacgcagctg cgtcgggctc gctatgccgc gcgtcgccgc
    17161 gaacgcctcc gcgagctcgt cgggcgtgaa gtaccggagc gcggcctcgt agacggtccg
    17221 cgcgtccgaa cgcagcgcca gcaccagcat catgttcccg aggtcgacgg cctggcgcca
    17281 cggcgagggc cggacctgga cgaagaacac gtcgatcacc ctcagctctc cgcgctgcac
    17341 catcaagttc gccggcttga tgtctcggtg ggcgagcccg acgtcccaca tcagtcggat
    17401 catcgcgagc gcttcgtcga tcacgtgcgc atctacggtg gcctcaccga tctcgaccgc
    17461 gtcgtcgaag aactccatcg cgatcaggta ctcgcgctcc ggggtgatct ccacgatccc
    17521 cagcggcgcc ggtgtgggga acccgaaccg gcccagcgtg ccgagcgtgt agtcctcgta
    17581 ttcgacgaac cgacggaccg tcgtgaaggg tgtctcgtcc tcgagccgtc cgtagagcat
    17641 cgtacggccg agcttgtacc aacggtccgc acgcacgtgg ctcttcgcgt agagcttcgc
    17701 gaagatcgtc cgctcgcggt cctgttcgtc ggtcacgcag agcttgagcg gggtcgaacc
    17761 cccggagccc tccagtccga ccggcttgac gtcgtgcacc gtgaacccga gctgatcccg
    17821 tagcgcggtg cggatggctt cgccccgacg acccgtgacg tcgaggtgcg cagacttccc
    17881 gtgcgcgccg taggcgaccg gaaagatgtc gttcgggacg aaggcgcgga acgcgaccag
    17941 cgggatcgag atcccgacga tcgcaccgaa cgcgacgtcg gtgagatggt cgacaccgag
    18001 gtagatccgc aacacgcccg tgatcgcgat ggcgcccgcc gcagccagct tcgcccagga
    18061 ccggggtcgg cccgggacga ccagcatgta cgccacgccg atcgtgagga tcgcgatcgc
    18121 gccgatcggg atcgacggcg tagcgtaccc ctcccagctc gcgagctgcc gcacgccgaa
    18181 cgggcgggga cgaagggaga ccagctcgag cccctggagg agcgctcccg cgacaccgag
    18241 agacaggaac cagatcacga gatggcgcca gcggcggaac cacacggtcg ccagcacgag
    18301 cagcaggccg agggccgcga atcccacgcg cgacccgacg gtgtgcgcct gacgggcgat
    18361 cgcgtcgagc catccggcgc ggatcgagat gacggcgtcg gtgatccacg cgtcgatgcg
    18421 atcgagcggg gcggtatcgg cacggagcca gaagcacccg gtgacgacca ccgcgagcag
    18481 caaggcgagc cagatccagc cggtggaacc gatctgtttc ggaagcggcg gggcggcgcc
    18541 cgtgggccga cgacgtcggt gtccgctgat ccgcgcatcg gcctgcgacc gcaccgtctg
    18601 gtcacgctga tccacgcttc cctccgcggg gatcgtcgca cgatcgtcca tggaccggga
    18661 gccgaggcgc tccatcttcc gtcggtctcc acccggcgac ctcggaggtg ggcgtctagg
    18721 gagccggtgc gccgggcttg aacaccatca gccagagcac gaccgcgagg aggagcagat
    18781 cgaggcgccc gatcatgagg aggcgatcca tcctccgccg gaactcggcg tccccgggac
    18841 ctcgatcgtc cgcgatccgt tcgatccgcc ttccctccgg tccgaagtac gccatgccga
    18901 ggaccatcga gacacccacc aacacgaggg aaagccagat ccaggtatct gagaaggcga
    18961 cccattcctc gatgacggtg atccatatcc ccgcaccgat caccacgatc gccgccgggc
    19021 cgatgaggaa cggcccgagc cagccgatct cgtgcgcaag tccagcgacg agggcgcgat
    19081 ccggattccg gctcgccctc gtcatgatgg cgctctgcgt gatcgaggca cccacccaca
    19141 cgatcgccgc gaggatgtgc aggaaggcga gccactcctg aagttccatc tatctcaccc
    19201 tttcgatcgc tcggaggccc tcgggcccac ccactccagc tgaaggaaaa ggcggaggaa
    19261 aaggcgctga aggaaaaggc ggaggaaaag gcggcacggt cgagcgcggc cggccctctc
    19321 catcatcgcg ctcatgggga tcaccgcccc gacccagacc atccgcgccc aggatgtgga
    19381 ggaatcagcc ggtcatggag ttccatggtc ctcctccccc aaccagcaca ggccggggaa
    19441 cagcgccgtt cgtggctcgc gtgtggtcca ggagcgatcg agcctgagtt gcccccttgc
    19501 ccttcaggtc caccacatag ggtcctgaga acgcgtagtt gccgctgagt cggtcgtaga
    19561 tggcctccga gacctggatg cttcccgggg ctgcgacgga ctcgaggcga ctcgccgcgt
    19621 tcaccgtgtc tccccagatg tcgtacgcga acttccggtg cccgatgacg cccgcgatga
    19681 ccgggccggt cgcgatcccg atccggaccg agatgggatc gccgctcggc catcggagct
    19741 gcgagagggt gtcgcggatc cggagcgcca tgtcggcggc cgcctccacg tgatccgtcc
    19801 tcaggtccgg cacgccggca acggccatgt aggcgtcgcc gatcgtcttg atcttctcga
    19861 gaccgaacct gtcggcaagc tcgtcccatc ggttgaacac ctcgtccaac gtgtcgatga
    19921 gctcatccgg gtccatccga gccgctcgtt cggtgaagcc gacgaggtcg gcgaagagca
    19981 ccgtggcctc ggcgtaccgc tcagcgatgc ggtcgtcgtc gaagccgggc tcacggatct
    20041 tcagccggtc gatgatgccc acgggaagga cgttgcgcag gagcatgtcg ccgcgctccc
    20101 gttcccgatc gagatcgcgt tctcgcagga acaagaggcg agcgaaccgt tcgagcgcga
    20161 acgccgccgc ggtcccgacc gtcgcgaagg cgaggaggtc gacgtcaggc tcgatcagcc
    20221 cgatgtcccc gggagcctgc acgagacccc gcatcgcgct gtagcagccg atcgcgagga
    20281 tgcctccgag cgctgcgtac cgatactgca gacggaagag ggcgaacgcg aaggccaaga
    20341 ccagcatgag agcgactaca ccggtccagt cggccggatg tctggtcacg aaccggtggc
    20401 cctgcgcgag ggcggcgctc acgaggacga ggacgacgat cgcgatctgc caccacctcg
    20461 catagccggg gacgaaggtc aacaggagac tgagtgaggt cacgccgatg cccaccagat
    20521 ggatggcgag gtacccctcc tgtccgatcg gcggtgcgga ggaaaaggcg aagaacgccc
    20581 acgcagcgat cccgaggacg agggcggcac gcacgtagcc caggttgtct cggaagtatt
    20641 gagcctggaa gtcgccttcg agacccgcct ccgtgaaccg gaggaagggt cggatccgag
    20701 gaaccgtcga tgaagaaccg atggtgctca tgtgagctcc ctttcgccgt ctgagggatc
    20761 gcgaagacgg cagatattcc ctagacgtgc gacgcgagca ctctcttccg ctcctcgaac
    20821 tcctcctcgg agatctcgcc tttggcgaaa cggctctcga ggatcgtctg cgggtccttc
    20881 gccccgccgg cgtcatgctg agacctcgcc ccgaacgccc gaacggcgaa gacgatcacc
    20941 gcgacgggaa cgccccagaa gaggagcatc atgatcgccc cccagaacca atcccatcca
    21001 tccatcacgt ccctcgcctc ctcagcaacg gcaataaccc ttggagcggt cagaccccag
    21061 ggtcgaaggt cccacggccg ccgggacctt tgccggcttg tacggggcgg ttagtgatcg
    21121 ctgggttggc ggcgtgctgc accgggctgg atcatgtgac gctgcgcccg tgatgccgcg
    21181 aaggagtccg gacaggctcc acgatccatc cccgcgtggc aggtccggaa ctagacgaaa
    21241 ggcgtcgaac tcgttcgtcc aagtgggccg atgcagagcc tgggagcacg aatcgcagtg
    21301 tggactgtcg aagctgcgag ggcgggaacg ctgtgagaag cccgagaatg gggtcggcgc
    21361 gtgtgggtga gcgctcgtga ctgaagctgg cgccaacccc cggtaggaga cctatccgtc
    21421 cgagcttacg gccgccttcc gggggtcttg atcgcgcggt gcatattcaa gtcaccgatc
    21481 gcgtccaggg cgtagacgcc gccgcgtcga cgaggaccgg acctgaaccg gggccccgcc
    21541 gcgccggtct ccgatcgtcc aggccgcgtc aggcatcgag gcgctcagcg cccaccgcga
    21601 gttagaaagt cccttccgag cggggacctt cggcccttcc ccgacggcgc ccgccgagga
    21661 tacgacacag ctctgaggga gaggtgaggg tccatggcca ggaccggggt ctcgcgggag
    21721 agccccgctc gtacgcacca cggggcggcc accgggccta ccacggttca cggtgactac
    21781 gtcccccgca ggtcccattt ctggcgccac ttcgtcgaga tggtcatcgc gatggtcgtg
    21841 ggcatggccg tcctcggttt cccgttccgg gcgatcctcg ggcagttcgg atacacgcga
    21901 gacgaagcgt tcgatcggtt cccggagatc gtctgcctcg tcatgacgtt caacatggcc
    21961 gtggggatgg tcgcgtggat gcggtttcgg gggcatccct ggcggataac cgcggagatg
    22021 accttggcga tgtgcgtggc gacggcggtc gcgctcgcca tgttctggct acacatcatc
    22081 gccgcggatc cgatgatcgc gttgatgcat gttctgatgc ttcccgcgat gctcgctgtg
    22141 atgctccgtc gtcgtgagga gtacgcccat gcgtatcggt gaccgcatcc acggacgttg
    22201 gacccacgac ggtgtcggcg atcgccctcc ggacgtaccc ctgcgaagcg agtcgcgcgc
    22261 ctgggcgcaa ctcggcttcg cctgcggcgt cctggcgatg gtggcgtaca ccatcctctc
    22321 ggccgttccg ctgtcgccgc agctccaggc ggtggcagtc tcgatcttcg gtcccgggat
    22381 cgccgtggcc tcggcgggcc tgtacgaagt cctgcggctc caccgatcga ccgtcggcct
    22441 gcggatcgct ctggtagcga acatcgcggc ggcgagcacg gtcacgatga tgctgttcgg
    22501 acagatcgcc ttcaagcggt ggctcgagct ggagttcccc acgggtccgg tcgccacgtc
    22561 gtcgtctccg gcctatcagg cggcgaacgg gctccagctc gggttcgacg tggtgtggga
    22621 cctgttcttc gcgatcggga cgttgctgtt cgcgctggcc atgtggacgc atccccggtt
    22681 cgggcgcggg ttcgccatca ccggaggcgt actcgccgtc ctgctgttga cgctgaacct
    22741 cggaaccttc ccggaacccc ccgagaaggc agggctcgtg gacgtcggcc cgctcgtggg
    22801 tctctggtac ctggccgcgg cggtccggat gcgttcgtcg ctccgatggg tcgatcgcat
    22861 ggcgagcggc tcggctcctc cggtgccgaa ggattgagga cgatggccac gcgtcagatc
    22921 acgatcacgc cgatgtcgcc ggaccgagtc gaggaggcgg gcagggtgtt gggcgccgcc
    22981 ttcgcctcca gtccgctcga gcgggccgtc cgcggaccga tcgacgaacg ccagcatcgt
    23041 ggtctcgtca tcgcctacac ggcgacgtgc cgcctgtcgg gtcaggtgcg gatcgccctg
    23101 agcgagcagc ggatcgtcgg agtgatccgt tgggttgagt cacctcgctg tcagctccgg
    23161 ctcggagaga agctggcgat ggcgccgact gcgatcggcg cgttcggccg caacctcccc
    23221 aaggcgttga tgtgggtctc caggtggtcg aagcgcgatc cgtcgcgacc gcatgtgcac
    23281 ctcgggccga tcggcgtgac gcccgagttg cagggccgcg ggatcggctc cgagatgctg
    23341 tcgctctact gcgaggaagt cgacctcgca gggaagcccg gctatctcga gaccgacaag
    23401 cgcgagaacg tccgtctcta tgaacggttc ggtttcgagg tccagaacga ggcggagctg
    23461 ctcggggtca ggaactggtt catgtggcgt gcgccgaccc ggcagcgctc cgtgacctga
    23521 gaggaggact ccatgaagag gggactgatc ggagcggtga tgttcctgct ggtcgtcgcg
    23581 ctgctccgtc gggtcgggcg gccgctgggc gagcgagcga tggagaagtg ccaggagatg
    23641 ttcgagcgtg ggatgccgga acgtcggggc ttgccatctg agcggaccgt cgctccggct
    23701 cggaggtgat cccgatgatg gacatgatgg acggagttgg ggacggctgg agctggatcg
    23761 ggggcgccct gatgatgctg atcctctgga gcgggctcgc ggctctgatc gtgttcctcg
    23821 tgcgagcctc cggtgggcgc ccatctcagg gtgaggacag gcaccagcgc tccgaggctc
    23881 gcgagatcct ggctgagcgt ttcgctcgag gtgaggtctc ggaggaggag ttcgagcaac
    23941 gaacgcgggt actccggcgc ggagggcggt cgagcagttc ccggggagga ggtagacagc
    24001 gatgatggga ggagaactcc tgatcgttct cggactcgtg gccgtggtgt tcttcgtcct
    24061 gcgaggctcc gatgccgccc ggtggcggga aggcggcgcg cctgaccagc cagacgctcg
    24121 agagatcctc gccgagcgct tcgcgcgggg tgagatctcc gaaggggagt tcga
//
LOCUS       DASUYD010000062        27337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_691780, whole genome shotgun sequence.
ACCESSION   DASUYD010000062 DASUYD010000000
VERSION     DASUYD010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 27337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691780"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..1405
                     /gene="sat"
                     /locus_tag="VF195_03365"
     CDS             248..1405
                     /gene="sat"
                     /locus_tag="VF195_03365"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
                     /protein_id="HEX6579891.1"
                     /translation="MPTIDPHGGTLVQLLVDGSAAETLREEARNLPTLVVSERELSDL
                     EMLAVGALSPLTGFPGEADYHSVLETMHLQNGLPWSIPVTLSVDEDGAHRLGGADAVA
                     LVAAEGSEPLAVLRISEIFKRDRAKEATSVFGTEDLEHPGVKALQDAGDRCVAGTLEV
                     LTLPPHEDFVAYRQTPAETRAEFERRGWRTVVGFQTRNPIHRAHEYIQKCALEIVDGL
                     LVHPLVGATKADDVPPDVRMRCYEALFEGYYPKDRAMVSVFPAAMRYAGPKEAIWHAI
                     CRKNYGCTHFIVGRDHAGVGDYYGTYDAQKIFERFEPGELGITPLMFEHSFWCDRCEG
                     MASPKTCPHGEEERVSLSGTKVREMLRDGERPPQEFSRPEVADILIEAMRG"
     gene            complement(1402..1944)
                     /locus_tag="VF195_03370"
     CDS             complement(1402..1944)
                     /locus_tag="VF195_03370"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6579892.1"
                     /translation="MELHPPFLTGRLVRLRAPEPSDDVLNELFNDHGVRSGLGAPFPQ
                     PVESFHDWIESARKKDDHVNLVIERIDEPGAIGMCDLMRIEAPTRTAEMGIWIGRPWW
                     RGGYGTDAVRTVCRFGFDHVNLHRITLFVNADNAQAIRAYEKVGFVHEGSLREAAFAH
                     GGRVDLLVMGLLAGDLREGS"
     gene            2093..3067
                     /locus_tag="VF195_03375"
     CDS             2093..3067
                     /locus_tag="VF195_03375"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6579893.1"
                     /translation="MIRSAVALLVSAAAVTAFPAWADTGSELEAAEARLDAAQAELDR
                     VTGLWQETEARLATARDAAAAAEAEIERLGDELTRIRRNLNDRVAAAFMTGGSFSIGA
                     LLTSDSIGDATDRLQYTQSVVQGDADLATEVAVTAEELRRQEARLRQSARIEAVASEA
                     LAEQRADLLLRIEDIDTLIADLEAELDAQQEATLGLGGDGVILRSGAIQTCPAAGTHS
                     FVDSFGDPRPGGRSHAGIDLMAAPGTPVIAVAAGNARPAGSIGGLGAVVEHPNGDWTF
                     YAHLSSYGTLGSVSTGTVIGYVGDHLHFEYHPNGGAAVNPYGMLLAVC"
     gene            3096..3626
                     /locus_tag="VF195_03380"
     CDS             3096..3626
                     /locus_tag="VF195_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aerial mycelium formation protein"
                     /protein_id="HEX6579894.1"
                     /translation="MDEEHPSNRRIDRIREPSYLDGIDSKSLEEVRALRDECMAEREY
                     LSLLRRLVQGRTEILKAELAARGSDDHRPLVDRLSEILASDEPATSRGEAMHVSLPEE
                     EMLLARRRIERLVADAGISDPTELDDPKLEEAIELLAGEEREVSAQRTDVLRVLDALQ
                     DELKRRYKEDPSLALS"
     gene            3761..4540
                     /locus_tag="VF195_03385"
     CDS             3761..4540
                     /locus_tag="VF195_03385"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HEX6579895.1"
                     /translation="MNLERGGSEAVTRDPIEDPEAGSLHDQLKVFAREVGVLYSAERV
                     RRRELEHALESAREMYVATMKSLAHVVEAKDPTTRGHLDRTARYGLTLARRVDPELAE
                     RPEVAYGFFLHDIGKVGIPESILCKTGPLTELEWVVMRSHPNQGARIVEPIPFMGEAV
                     EIVRCHHERFDGEGYPTGLSGEDIPLAARIFSIADSFDAMTSDRPYRSALSLDEAVAE
                     IRAGAGTQFDPRCVEAFEELAAEEDVLVLPDRRRTPSLATG"
     gene            4582..5547
                     /locus_tag="VF195_03390"
     CDS             4582..5547
                     /locus_tag="VF195_03390"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6579896.1"
                     /translation="MAAPAALVHVVRNGLVESVHMGDVAVCDADGTLRAAAGDPDRLL
                     FGRSCEKPLQGAVSFSAMDEPPLPDDEVAVMCASHSGEAIHVAAVRRLLRRGPVPVGA
                     LKTPRDRATKGARARIWDGCSGNHAGLLVAAARRGWDLATYRAPGHPVQRRVLRAVSA
                     ATDVERPIIGVDGCGIPVHGVPLRAMATMFARFGSPDRMGRLATATDRVVRGILAAPH
                     LVGGTRRLDTDLITAGAGSVMAKEGAEGLVCATSLPQGIGIAVKVTDGNWRRLAPAFI
                     EVLRTLDVLPPGALDRLAHHERLPVLGGEEPQGAVQAVVRLRRPR"
     gene            5623..6870
                     /gene="mshA"
                     /locus_tag="VF195_03395"
     CDS             5623..6870
                     /gene="mshA"
                     /locus_tag="VF195_03395"
                     /EC_number="2.4.1.250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010125 - mycothiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-inositol-3-phosphate glycosyltransferase"
                     /protein_id="HEX6579897.1"
                     /translation="MSIGATRPRVAVLAVHTSPVDQPGTGDSGGMNVYIRSVAERLAG
                     QGIEVDLFTRCRGGATHDVREIAPGIRVVSAKAGPCQPLPKSAVQRYLPEFLGGVLRV
                     ARENGNRYDLIHSHYWLSGWVGRAAKDILGVPLVASFHTLGKVKNYSLAHGETPEPAE
                     RLRGEERVIGEADRVLAPTPEEAGQLVGLYGADPERVRIVPPGVDHAIFRPQDRDVSR
                     ARLHLSGLRLALFVGRLQSHKGPDVAIRTIAEVVARSPALAEDLVLAIVGGPSGQHAV
                     AEVARLMELATALGVADRVMLFPPQPHARLSDFYSAADVVLVPSRSESFGLVALEAQA
                     CGTPVVAAAVGGLRTVVRGGGLLVEGHDAADHGEAVRALLGDPALSERLGTTGARESL
                     AFTWDATTMEVRSIYQELVEAAA"
     gene            6867..7415
                     /locus_tag="VF195_03400"
     CDS             6867..7415
                     /locus_tag="VF195_03400"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6579898.1"
                     /translation="MIHGAQVLLRPVEERDHVLIHAWQNDPEVWWLMDYEGPFSMQDV
                     VESEQRARAEGHPYVIEVDGTPIGRIGLNAFRRRDRICSLYVLIGERSAWEQGYGTDA
                     VSALLDEAFDRYDLHRVELWSTADNERAIHVYEKCGFVVDGRLPERSWKGGAWVDRVV
                     MSVTREDFAASATGSDLRRRGH"
     gene            7998..8612
                     /locus_tag="VF195_03405"
     CDS             7998..8612
                     /locus_tag="VF195_03405"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579899.1"
                     /translation="MDESRRDRILEAAMRRFADRGYRGTRVEDVANEVGVAKGTVFLD
                     FGSKEGLFLEAFKRAVSLLPAWLDAPADVVDQGFWAVLDWWLQRAEDLVVSDPVPNRV
                     ALIARYDTSLDIRRPVDRFMRSEDPYGTLEFVEFGVKRGELRDDVDAEMIASTLDWMA
                     ERFQDALVSEDLDPGLIHRRPERRPMRIKEFVEVLRGGIAPQPE"
     gene            8686..9513
                     /gene="proC"
                     /locus_tag="VF195_03410"
     CDS             8686..9513
                     /gene="proC"
                     /locus_tag="VF195_03410"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018548048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX6579900.1"
                     /translation="MSERSIAFLGGGRMGEALVAGLLRAGARTEDEVLVTCRREERAQ
                     ELTERHGVATTLDNPEACRFAGVLVLMAKPQDFEVLLQQIADHVTTEHLVVTFAAGIR
                     TSFVEQHLAPDVPVVRVMSNVPVMVDEAMSVIAAGRHADDKHLAVAEELLAAVGKVLR
                     LEERYLDAVTATSGSGPAYFFLLAEAMIEACILLGLSRDVASQLIIQTMLGSAKMLRD
                     TGTHPVELREMVTSPGGTTIEAIRHLEEAGVRAAFLNAIDAARRRSEELARGGDEDD"
     gene            9535..9660
                     /locus_tag="VF195_03415"
     CDS             9535..9660
                     /locus_tag="VF195_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579901.1"
                     /translation="MGRGDLAKVRDARDRGRKKKEREKRQAAERAAARKEAKKKK"
     gene            complement(9664..10470)
                     /locus_tag="VF195_03420"
     CDS             complement(9664..10470)
                     /locus_tag="VF195_03420"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX6579902.1"
                     /translation="MRLVTYDRRGHRRLGAILDGRVVDLPDAVGHPAFPTTLEAFVQS
                     SRGTVLDAARAALARDDVKDHVVRQARVLPPLFPRSLLLPGGPERGEGGEGLERRIAG
                     PEQDIAWPAGAAWLDYEPKVAAVLAIETKDVETDAVAEHIFGYTLVSDWVAKDASGDP
                     VGTSEGVPIAIGPCVVTVDECDPQAMFLQAKVDGGELAKGNLNGSARSLFRLIAAASR
                     GTTLERGDAFALGPFPLRDDDPDRRLWPGALVELAAEGIGTLRNRLGARP"
     gene            10631..12196
                     /locus_tag="VF195_03425"
     CDS             10631..12196
                     /locus_tag="VF195_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010146430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6579903.1"
                     /translation="MAEKFRITYATLSADNEDLQRNYDEAVERARAELGKEWPFVVNG
                     EERWSEEKYEEPSPIDEDLIIGRFSQATPQDVDDAVEAAKAFSLEWERIGWQERVRIL
                     RNVADVMEDRLFDLAALMAFEVGKSRLEALGDVQETAELIRWNCDEVEKHEGFRTPMS
                     GLGSPGDYYDVMRPYGVWAVISPFNFPFALAGGPSSGALVTGNAVVLKPSNQGALMGY
                     KLYECYRDGGVPPGAFHLVVGRGALAGEALWRHRDVNGITFTGSYAVGMDILKNFVQD
                     VPKPVIAEMGGKNPAIVTKHADLDVATDGVLRSAFGFGGQKCSANSRVYVEREVYDDF
                     VRLLKEKTEKIKVGNPIERDVFLGPVVNHEALDTFEQAAGEAKKNGTVITGGERITAG
                     DLGRGLFVQPTIVEAPEDSWLWKKELFVPFLAVAPFDELDDAIDKANDTEYGLTAGFF
                     SKDRHEIEEWLDKIQAGVVYVNRRAGATTGAWPGVQPFGGWKGSGTTGKAGGGPYYVL
                     QYLREQSRTVIEE"
     gene            12193..13566
                     /locus_tag="VF195_03430"
     CDS             12193..13566
                     /locus_tag="VF195_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018567239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HEX6579904.1"
                     /translation="MSLEDLELEGLANESAPRLITEIPGPRAQEVVERDRATTSPSLP
                     RAYPFAPKRASGSVVEDVDGNLFLDLNAGIAVTSTGHCHPTVVEAIQRQAAELIHYSA
                     SDFFLPIYSELTERLDQIAPMSGRVRSFLTNSGTEAVEAAMKLARHHTGRQYLLAFYG
                     SFHGRSYGSVSMTASKSSYRAGFGPLLPGVLHAFYGDGRDIGFAPGSGSEGFPTIDYI
                     EGFLMKKIVAPTEVAAIVVEPVLGEGGYIVPPDGWLQALRELCDRHGIMLVCDEVQSG
                     MGRTGKMWAVEHFDVEPDILLAGKGIASGMPLGAMIARDDRMTWTIGTHGSTYGGNAV
                     SCAAALATIGVIQEELLLENAAKVGDQLMNGLRDIQSRHDTIREVRGLGLMIGVEFPD
                     HGTAAAVERGAFERGLLMLGCGESAIRLSPPLVLREDQARVGLDLFEEVVAAAAPGVG
                     AVPDSGA"
     gene            13581..13958
                     /locus_tag="VF195_03435"
     CDS             13581..13958
                     /locus_tag="VF195_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6579905.1"
                     /translation="MPLGLDKVTFDCADTMRVARFWAEALGFDLDPESEPDGALIRDP
                     SGRSDGIYFQPVPESKIAKNRLHLDLRPTGSMAAEVERLEGLGATVVGRVDEGGSFWT
                     VMQDPEGNELCVLRGPEDGWNAG"
     gene            13955..14875
                     /locus_tag="VF195_03440"
     CDS             13955..14875
                     /locus_tag="VF195_03440"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6579906.1"
                     /translation="MTSKVATMHEAVADLVRDGDTVAIEGFTHLICFAAGHEIIRQRK
                     RDLTLARMTPDVIYDQMIGAGVARKLIFSWLGNPGVGSLHAVRRRIEDHDPAPLEIEE
                     YSHFGMVCRYVAGASNLPFFPIRSYYESDIPGVNPKIVPMTSPYEDGSEVYVVPPLKP
                     DVTVVHAQRADRDGNAQIWGLLGCQKEAAFAAERVIVVVEELVEGDVIRRDPNRTVIP
                     GIVVDAVVEEPFACHPSFAQGYYDRDNEFYLAWDAIARDPESLDAWLQEWVYGLASHA
                     DYVERWGAEHWAALKPGPALSGEVDYGSYA"
     gene            14872..15666
                     /locus_tag="VF195_03445"
     CDS             14872..15666
                     /locus_tag="VF195_03445"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004214463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HEX6579907.1"
                     /translation="MSVPSSDDDGRARYSKNELMIAASARLLAGVENCFVGVGLPNIV
                     CNLAQRTVAPELQLVYESGVFGARPRRLPLSIGDPTLATGSTAVTSMFELFAFYLQAG
                     LIDVAFLGGAQIDRFGNLNTTVIGDYAQPKVRLPGSGGACEIAIHARKILVIMRQAER
                     SFVQKLHFRTSPGHSGDPAHDRERGWSGSGPTNVVTDLATYAFDVETGEMTLHTLHPG
                     VRLEDVRANMAWEPRVARDIAETPPPTSEELRLLREELDPGRIYTK"
     gene            15677..16984
                     /locus_tag="VF195_03450"
     CDS             15677..16984
                     /locus_tag="VF195_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015801880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6579908.1"
                     /translation="MSHVFSRSSLDLPTAVSADGAWITDADGTRYLDGAGGAIVVNVG
                     HGVASVIAAIGEQLERTQYVHPTAFTTRAVEAYAEEVAGILPMDDARIYPVSGGSEAV
                     ETALKMARAYHLAQGRGGRSIVIGRRGSYHGNTLGALDASGKAPLRKPYTPWLGRFLH
                     VSAAYEFRCENPQHPHGCGAWQAAELEKLIASYGEDTVAAFIGEPISGATLGAAVPTD
                     DYWPAIAEVCRAHDVVLIADEVMTGFGRTGRWFGMDHWDIRPDILTAGKGTTSGYVPF
                     GFAAASGQIHEAIAGGPGFVHGFTWSHNALGAAAGLAVLGELKAGLIDRSRELGARLL
                     DGLRSELAGAPAVGDVRGLGLLIGIELVRDRETNEPFLRADGVSERVLQAARESGLLL
                     YSSTGHVDGANGDLVLLGPPLSLSDDEASLLLERTVAAVLSVP"
     gene            16981..18123
                     /locus_tag="VF195_03455"
     CDS             16981..18123
                     /locus_tag="VF195_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HEX6579909.1"
                     /translation="MSGDVALVRCPEARIYDHGPGHPLRPDRVLLTWDLIAATGLDQR
                     EGVEVLNCEPASDDTLRLVHTKEYLEATKAAGSGVEGDWSRFGYGPGDNPIFDRMHEA
                     GALVAGASVEAVRAVWSGDVLHAFNAAGGLHHAMPARASGFCVYDDPAVAIAWLLREG
                     AERVAYVDVDVHHGDGVQSIFWDEPRVLTISIHEYVPGMFFPGTGAASERGGPGAKGS
                     AINVPLPPETGDDAWLDAFRAVVPPAVERFAPDVLVTQLGCDTHATDPLAELRLTTRA
                     YRGCAQELHALAHRTAAGRWVATGGGGYQWARVVPRAWTLAFAEMAGALADLPDQLPE
                     GFIEHAERRLGDEVPATFSEPDLGPGRGDDDARAAADEVRRLVLGP"
     gene            18120..19535
                     /gene="pyk"
                     /locus_tag="VF195_03460"
     CDS             18120..19535
                     /gene="pyk"
                     /locus_tag="VF195_03460"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:TIGR01064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX6579910.1"
                     /translation="MSARTKLVCTLGPASATPKMVRGLVEAGASVFRINCSHGTREDH
                     ARSVSIVREVEGATGRSLSVLADLPGPKVRLADVHPDPFVFRPGQPFEIRPDGPGDAT
                     GAATSYAGLADDLRVGDRILLADGAVELTVTGTEGDRVRTECVRAGTVRSHQGVNVPA
                     ERLSLPPVTEKDREMLRHVLDLGVDLVAQSFVRAPSDVEELRAAMGDRPVPIVAKIET
                     RPAVEGFERILEVSDAAMVARGDLGVELPMEEIPLVQKRLLRAARVADRPVIVATQML
                     ESMIRSPRPTRAEANDVASAVLDGADAIMLSGETAIGEFPFEAASVATRIARAVESQG
                     AEYRAAQPPCLHSGEAAAVAHAAASIVQGDSDAVAITCYTTTGRTAALLSAERPRVPI
                     HAFIPDVSVRRANGLLWGVEAHGAERPDDTDQMISMMDERLRAAGLAADGDTVVMAAS
                     MPAGRTTTNMLKVHQVGSLEA"
     gene            19632..19817
                     /locus_tag="VF195_03465"
     CDS             19632..19817
                     /locus_tag="VF195_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579911.1"
                     /translation="MALRSPNEIPGRPSGVLVPTYSDNEHPGTKAEHRRLAVEWRRRH
                     PQWWKRLLRLRSVPPAD"
     gene            complement(19797..20318)
                     /locus_tag="VF195_03470"
     CDS             complement(19797..20318)
                     /locus_tag="VF195_03470"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HEX6579912.1"
                     /translation="MEPAFSKEGDRFAVRLSRGVRGLLIELCRQSRALLDAEDPSSDP
                     AVSRLFPPAYPDDPLQNLEFETNLGAAPRAGKLEALDVVERTANASALTEDELLAWIG
                     VVNDLRLVLGTRIEITEEATDEDFPGDDPRHETYRIYQFLGFLLQELLEGIGAPDVGE
                     PRNEGEDSVGGGD"
     gene            complement(20318..20626)
                     /gene="clpS"
                     /locus_tag="VF195_03475"
     CDS             complement(20318..20626)
                     /gene="clpS"
                     /locus_tag="VF195_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEX6579913.1"
                     /translation="MTSLRTTTTRPGRVQQPHVDEVDEPDLPWIVIVWNDPINLMGYV
                     TWVFMRLFGYPREKAERLMWDVHTKGRAVVSSGPREKAESDVARLHAHGLWATLQRDA
                     "
     gene            complement(20623..21381)
                     /locus_tag="VF195_03480"
     CDS             complement(20623..21381)
                     /locus_tag="VF195_03480"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6579914.1"
                     /translation="MVAGASSGIGEAAALAFADRGARVVLAARRTARLDALVERIERS
                     GGKALAATCDVTEPEQLTTLRVVTEEVYGPADVLVNSAGMRGGGEFTKLDYDRIEDHV
                     RLNVLGVLFGTRAFLPSMLARGKGHIVNIGSLAGRFATPGNAIYGATKHAVVAFSEAM
                     DYETEGRNVHVTSVNPGFVDTEGFPQRDLPDWAVLKMRTVTDAIVKVVREDIAPEYSV
                     PRWLSPFQLFRVATPPLYRWGIRRIRRARRSTSR"
     gene            21435..22268
                     /locus_tag="VF195_03485"
     CDS             21435..22268
                     /locus_tag="VF195_03485"
                     /inference="COORDINATES: protein motif:HMM:NF027034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HEX6579915.1"
                     /translation="MDVIGAHAGRLPVLDERWEPRPYPREELERTLVAGGVAGRAGHP
                     HHNVFGNIRKLVEHDPDKLLGLSNVPEPFGFEDIVRIVGEASGVPIDALATEGSPDIA
                     AGSVMDACEAVGERLAIAARRRERVLFATGHPGDLDPLYGAIAELATARGAAIVRPAE
                     GETWFDDHAGTEWTIGYHGAVGMITDGERPRHTHWPEAMRRMLADERPDLVMADHGLA
                     GAAIEAGVETLSIADVNDPALIVAKAQGRTEIVVVLDDHVPPENYWPCFQAVAGRFDP
                     S"
     gene            complement(22265..22570)
                     /locus_tag="VF195_03490"
     CDS             complement(22265..22570)
                     /locus_tag="VF195_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579916.1"
                     /translation="MTDTRRALLRRLEHANAFPLVALQALRELRTDLDAVEAEAMLRA
                     RELGASLEDIGDALHITRQGVSYKLKSLAGTGHDDDGPAEDEIIDVREPDTRGARSD"
     gene            22982..25810
                     /locus_tag="VF195_03495"
     CDS             22982..25810
                     /locus_tag="VF195_03495"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX6579917.1"
                     /translation="MLSAALTLFTVLGMTGALADNLQNDVTAGGNDTITAGGSTTITY
                     RLIGNNAPSGDVSGCNVDASNPATVTISLPGAVTESPSSFQFTACGNAGAKIVTYGSS
                     TGGDYLINVSISGGKPGSLYNDQADLTLHVNAAPVVDTDGDGVVDSADNCLTVANAGQ
                     EDSDGDGLGDVCDPDTDGDGVANASDNCPGVANPGQADTDGDGLGDACDPLTDSDGDG
                     IGDSVDNCPNVADADQADADGDGLGDACDPDTDGDGVLNVADNCPAVPNADQADADGD
                     GAGDACDPDTDGDGVLNGVDSCPTVANADQVDVDSDGLGDACDSDRDGDGVPNATDNC
                     PDDPNPGQADADGDGLGNLCDPNAFAPQIVDDADDVNGFEAVELTNSGSFSDGDSDTL
                     AITKVSGAGALVDDGDGTWEWSFTPGDDASGSVVVQASDGDHAPTTDSFDWTATNSPP
                     SISVVSNNGPINEAASASITVTATDPAGSNDPLAYSFDCDHDGTFGAAGSSNQGSCSF
                     GEDGTYTVGVKVTDGDGGEDTDSTSVSVLNVTPTLDSLTLTPSSINETGSTTLAAAVS
                     DPGADDSELSYSISWGDGSADTTGSTSTGAISESHAFADDDADDSYGVTLTVEDSDGD
                     ADSDTANVTVSNLPPSISVVSNSGPINEGSSATITITATDPAGSNDPLAYSFDCDNDG
                     TFGAAGSSNQGSCSFAQDGTYTVGVKVTDGDGGLDTDSTGVSVLNVAPSVSLTLTSAS
                     GTCAANTASLNLSFTDPGADAPWDATLNWGDGSTEVLSLATPGTSTQLHPYASAGQYT
                     ISVTVKDDDVASASATIEFTLNYDLSTILQPINDTRNGQPTSLFKYGSTIPVKVVITD
                     CDGSLPTDLAPKVTWRQGLNATPTGIDEAVPTSQADLGNTMRFSGGQYVLQLNSKNTT
                     SDPSCGVTIWVTIPTTGQTVQANIGFKK"
     gene            26046..26246
                     /locus_tag="VF195_03500"
     CDS             26046..26246
                     /locus_tag="VF195_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6579918.1"
                     /translation="MVMPEPFTEARLLTANEVADLMRVSRMTVYRLIKTGEMPSLRVG
                     RGYRFREEDVHDYLRGRYTEAG"
     gene            26312..27202
                     /locus_tag="VF195_03505"
     CDS             26312..27202
                     /locus_tag="VF195_03505"
                     /inference="COORDINATES: protein motif:HMM:NF027889.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide 5'-hydroxyl-kinase"
                     /protein_id="HEX6579919.1"
                     /translation="MNEHDRLVDRAAREARAVLFVGGLDSGKSSMARATAAYALTLGR
                     SVAYLDADVSQKTVGPPGTVGLKHIREPDDLTHDRMAQADAIGFVGSTSPQDHLLPLV
                     GALSRLRERAHDEGAELVVVDTGGEVSGIRGQLVKYYKVDTLEPDLVVALQRGEELEP
                     IVGVMERFFGVEVVRLGIHPAVVPTTVDERMAQREESMRRYFAQPLQRFRVRPTVFMP
                     SLPPLFDLSQLDRLLVGLSDGEGGFTGVGYLEPVSGNGGLRLISPVAEAPKALRLGSV
                     RLEESFRAKRIDLRNLFGTE"
     gene            27216..27317
                     /locus_tag="VF195_03510"
     CDS             27216..27317
                     /locus_tag="VF195_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003402602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AURKAIP1/COX24 domain-containing protein"
                     /protein_id="HEX6579920.1"
                     /translation="MPSLVKKRRKKMRKKKHKKLLKRTRHQRLKLGK"
BASE COUNT         4290 a         9253 c         9647 g         4147 t
ORIGIN      
        1 cggctgcaac gtctcgaagt cgacgttcag tgaggcgatc tcgtcatcgg aaaggagggt
       61 ctgcatcggc accgaaggcc ttgggtcgga accgtcagtc taccccgctc gccggatgcg
      121 gcctcaggcc gagagtcagg aagggctgcg cggccgacca acccgtatcc tgggagttcg
      181 accccgcggc ctcaccgctc cgggtctccg tcgtctacgg ccatcgtcta cggaaggaag
      241 aacgtccatg cccaccatcg atccgcacgg aggaacgctc gtgcagctcc tcgtcgacgg
      301 ctccgccgcc gagaccctcc gcgaggaggc ccgcaacctg ccgaccctcg tcgtctctga
      361 acgggagctc tccgatctcg agatgctcgc cgtcggggcg ctctcgccgc tcaccggatt
      421 cccgggcgag gccgactacc actcggtgct cgagacgatg caccttcaga acggcctgcc
      481 ctggtcgatc cccgtcacgc tgtcggtcga cgaggacggt gcgcaccggc tgggtggcgc
      541 cgacgcggtg gcgttggtcg cggccgaggg gagcgagccg ctcgcggtcc ttcggatcag
      601 cgagatcttc aagcgcgacc gggccaagga ggcgacgtcc gtcttcggga ccgaggacct
      661 cgagcacccc ggggtcaagg cgctccagga cgccggcgac cgttgcgtgg cggggaccct
      721 cgaggtgctc acgctcccgc cccacgagga cttcgtcgcc tatcggcaga cgccggccga
      781 gacccgggcg gagttcgagc ggcggggctg gcgcaccgtg gtcggattcc agacgaggaa
      841 cccgatccat cgggcgcacg agtacatcca gaagtgtgcc ctcgagatcg tcgacgggct
      901 tctcgtccat ccgctcgtgg gagccacgaa ggccgacgat gtcccgcctg acgtgcgcat
      961 gcgctgctac gaggcgctgt tcgagggcta ctacccgaag gaccgggcga tggtctcggt
     1021 gttcccggcg gcgatgcgct acgcggggcc gaaagaggcg atctggcacg cgatctgccg
     1081 gaagaactac ggctgcacgc acttcatcgt cggccgagac cacgccggcg tcggggacta
     1141 ctacggcacc tacgacgcgc agaagatctt cgagcgcttc gagccgggcg agctcggcat
     1201 cacgccgctg atgttcgagc actccttctg gtgcgatcgc tgcgagggga tggcctcacc
     1261 caagacgtgc ccccacggcg aggaagagcg ggtctccctc tcggggacga aggtgcgcga
     1321 gatgctgcgc gacggcgagc ggcctccgca ggagttctcg cggccggagg tcgccgacat
     1381 cctgatcgag gcgatgcggg gctaggaccc ttctcggaga tcacccgcga ggagtcccat
     1441 cacgagcagg tcgacgcgtc ccccgtgggc gaacgcggcc tcgcggaggc ttccctcgtg
     1501 gacgaacccg accttctcgt aggcccggat cgcctgagcg ttgtcggcgt tcacgaacag
     1561 cgtgatgcgg tgcaggttca cgtggtcgaa accgaaccgg cagacagtcc gcacggcgtc
     1621 ggtgccgtag cctccgcgcc accaaggcct gccgatccag atccccatct ccgccgtccg
     1681 ggtgggcgcc tcgatcctca tgaggtcgca catgccgatc gcgcccggct cgtcgatccg
     1741 ttcgatcacg aggttgacgt ggtcgtcctt cttccgggcg gactcgatcc agtcgtggaa
     1801 cgactcgacg ggctgtggga acggcgctcc gaggccgctc cgcacgccgt ggtcgttgaa
     1861 gagctcgttc aggacgtcgt cggatggctc cggcgcccgg agccgaacga gccggccggt
     1921 caggaacgga ggatgcaact ccaccggcgg aggatagatg ccgaagaaga gggaacggac
     1981 ggcgcagccc gagggagttg agccccgacc ggagtcactg gtagcctcgg tcgcgccgtc
     2041 gatgccccgt cggatgcaca gggaggcaac agccacggcc ccttcccgca gagtgatcag
     2101 atccgccgtc gcgctcctcg tgagcgccgc tgcggtcacc gcgttccccg cctgggcgga
     2161 caccggatcg gagctcgaag ctgcggaagc gcggctcgac gccgcccagg ctgagctcga
     2221 cagggtcacg ggtctctggc aggagaccga ggcgcggctc gcgaccgctc gggatgcggc
     2281 agccgcggcc gaggcggaga tcgagcgcct cggggacgag ctcacgagga tccgcaggaa
     2341 tctgaacgac cgggtcgcgg ctgcgttcat gacgggcggg tcgttctcga tcggcgccct
     2401 cttgacgtcg gactccatcg gggacgccac cgaccgactg cagtacacgc agagcgtcgt
     2461 acaaggcgac gccgacctcg cgacggaggt cgccgtcacc gccgaggaac tccgtcgcca
     2521 ggaagcccgc ctccgccagt ccgctcggat cgaggccgtc gcgtcggaag cgctcgcgga
     2581 gcagcgggcc gacctgcttc tccggatcga ggacatcgac accctgatcg ccgacctcga
     2641 agcggagctc gacgctcagc aggaggccac gctcggattg ggaggcgatg gtgtcatcct
     2701 ccggtccggc gcgatccaga cgtgcccggc ggccggcacg cactcgttcg tcgactcctt
     2761 cggcgatcct cggccgggcg gtcggtcgca cgcgggcatc gacctcatgg cggcgccggg
     2821 gacaccggtc atcgccgtcg ctgccgggaa cgcccgaccg gctgggagca tcgggggcct
     2881 gggcgctgtc gtcgagcacc cgaacggcga ctggaccttc tatgcccatc tgtcctcgta
     2941 tggcaccctc ggttccgtgt cgaccggaac cgtgatcgga tacgtcggcg accacctgca
     3001 cttcgaatac cacccgaatg gcggcgcggc cgtgaatccg tacgggatgc tcctcgccgt
     3061 ctgctgagca cgggggccgg gtgtagcatc gcctcgtgga cgaggagcat ccgagcaacc
     3121 gccgcatcga tcggatccgc gagccctcgt acctcgatgg gatcgattcc aagtcgctcg
     3181 aggaagtgcg ggcgctccgc gacgaatgca tggccgaacg cgaatacctc tccttgcttc
     3241 gcaggctcgt ccagggccgc acagagatcc tgaaggccga gctggccgcc cgagggagcg
     3301 acgaccatcg gccgctcgtg gatcgactct cggagatcct cgcgtccgat gagcccgcga
     3361 cgtcacgtgg cgaggcgatg cacgtctccc tgcccgagga ggagatgctc ctcgcgagac
     3421 gccggatcga acgtctggtt gccgatgcag gcatcagtga cccgacggag ctggacgatc
     3481 ccaagctcga ggaagcgatc gagctgctcg cgggcgaaga acgggaggtt tcggcacaac
     3541 gaaccgacgt gctccgtgtg ctcgacgcgc tccaggacga gctgaagcgg cggtacaagg
     3601 aggatccgag cctggcgctc agctgaggtt ccgcaacgtt ctggtggcgt cccccggctg
     3661 ccggccacga accccccggt cgcccgatac cccgctccgg gagcccgtgc gaacgtgtgc
     3721 cccgcttctc cacaactggg gataacgagg acgggggaac atgaatctcg aacgaggggg
     3781 atccgaggcc gtcacccggg atcccatcga ggatcccgag gcggggtcgc tgcatgacca
     3841 gctgaaggtg ttcgctcgcg aggtcggcgt cctgtattcg gcggaacggg tgcgccggcg
     3901 cgagctggag cacgcactcg agagcgcccg cgagatgtac gtggccacga tgaagtccct
     3961 cgcccacgtg gtggaagcaa aagaccccac gacgcgggga cacctcgatc gcacagcgcg
     4021 ctacggtctc acgctcgcac ggagggtcga ccccgagctc gcggagcgac cggaggtcgc
     4081 ctacgggttc ttcctccacg acatcgggaa ggtcggtatc cccgagtcga tcctctgcaa
     4141 gaccggcccg ctcaccgagc tcgaatgggt cgtgatgcga tcccatccga accagggcgc
     4201 acgcatcgtc gaaccgatcc ccttcatggg tgaggccgtc gagatcgtcc ggtgtcatca
     4261 cgagcgattc gacggggagg gatacccgac gggactcagc ggagaagata tccctctcgc
     4321 cgcccgcatc ttctcgatcg cggactcgtt cgatgcgatg acgagcgacc ggccctaccg
     4381 gtcggcgctt tccctcgacg aggccgtcgc ggagatccgc gccggtgccg gcacgcagtt
     4441 cgacccacgc tgcgtggagg cgttcgagga gctcgcagcg gaggaggacg tcctcgtcct
     4501 ccccgaccgg cgccgcacgc catccctcgc cacgggttga gcccggccgc ccccgacggg
     4561 cgtcggtagg ctctcgctcc gatggccgct cccgccgcgc tcgtccacgt cgtccgcaac
     4621 ggcctcgtcg aatcggtgca catgggcgat gtcgccgtct gcgacgccga cggtaccctc
     4681 cgcgccgcgg ccggcgatcc cgatcgtctc ctgttcggtc gctcctgcga gaagccgctg
     4741 cagggggccg tctcgttctc ggccatggac gagccgcctc tcccggacga cgaggtcgcc
     4801 gtgatgtgcg cgtcccactc cggcgaagcc atccatgtgg cggccgtgcg gcgcctgctg
     4861 cgccggggac ccgttcctgt cggcgccctc aagaccccac gcgaccgcgc caccaaggga
     4921 gcccgtgcac ggatctggga cggctgttcg gggaaccacg ccgggctgct ggtcgccgcc
     4981 gcgcgccggg ggtgggatct ggccacctac cgagcgccgg gtcatcccgt ccagcgacgc
     5041 gtgctgcggg ccgtctcggc cgcgaccgac gtcgagcgcc cgatcatcgg cgtggacggg
     5101 tgcgggatcc ccgtccacgg cgtgccgctc agagcgatgg caacgatgtt cgcccggttc
     5161 ggctctccgg atcggatggg acggctcgca acggcgacgg accgggtggt gcggggcatc
     5221 ctcgccgccc cgcacctggt gggcggcacg cggcgcctgg ataccgacct gatcacggcc
     5281 ggagcgggtt cggtgatggc gaaagagggg gccgaggggc tcgtgtgcgc gacgtccctg
     5341 ccgcagggga tcggcatcgc ggtgaaggtc accgacggca actggcggcg gctggcgccg
     5401 gcgttcatcg aggtgctgcg gacactggac gtgctgccgc cgggcgccct ggaccgtctg
     5461 gctcatcacg aacggctccc cgtgctgggc ggcgaggagc cccagggggc cgtgcaggcg
     5521 gtcgtccgcc tccggcgacc tcgatgaggc ggccgagagg atcgctcggc tcgctcgtcg
     5581 aagcgggatg aaatccggtc cctcgggagg ttgtaggtgt ccgtgagcat cggagcaacg
     5641 cggccgcgcg tcgccgtcct cgccgtgcac acctcacccg tcgaccagcc ggggaccggt
     5701 gactcgggag ggatgaacgt ctacatccgt tcggtcgccg agcgcctggc cgggcagggg
     5761 atcgaggtgg acctcttcac gaggtgccgg ggcggggcca cgcacgacgt tcgcgagatc
     5821 gcgccgggca tccgggtcgt gagcgccaag gcgggtccgt gccagcccct gccgaagtcc
     5881 gcggtccagc ggtacctccc ggagttcctc ggcggcgtcc tgcgcgtcgc ccgcgagaac
     5941 ggcaaccgct acgacctgat ccacagtcac tactggttgt ccgggtgggt cgggcgcgcc
     6001 gcgaaggaca tcctcggcgt gccgctcgtc gcgtcgttcc acacgctcgg caaggtcaag
     6061 aactactcgc tcgcgcacgg ggagacgccg gagccggccg agcgactccg tggcgaggag
     6121 cgggtgatcg gggaagcgga ccgcgtcctc gctccgacgc cggaggaggc gggccagctc
     6181 gtgggtctct acggcgccga tcccgaacgg gtccggatcg tgcctcccgg tgtggatcac
     6241 gcgatcttcc ggccgcagga tcgggacgtc tctcgagcgc gtctgcacct gtccggtctg
     6301 cggctcgcct tgttcgtcgg tcgtctgcag tcccacaaag ggcccgacgt ggcgatccgg
     6361 acgatcgccg aggtggtggc gaggagtccg gcgctggccg aggatctcgt gctcgcgatc
     6421 gtgggtggcc cgagtggtca gcacgccgtt gcggaggtcg cgcgcctgat ggagctggcg
     6481 accgccctcg gggtggccga ccgcgtgatg ctcttccctc cgcagccgca cgcccggctc
     6541 tccgacttct actcggcagc cgatgtcgtg ctcgtgccgt cgcgatcgga atcgttcggc
     6601 ctcgtggcgc tggaggcgca agcatgtggg acgcccgtcg tcgcggctgc cgtcggcggt
     6661 ctccggaccg tcgtgcgggg aggcgggctg ctcgtcgaag gacacgacgc cgccgaccac
     6721 ggcgaggcgg tgcgcgccct cctcggcgat cccgcgctct ccgaacgact cggaaccacc
     6781 ggtgctcgag agtcgctcgc gttcacgtgg gacgccacga ccatggaggt ccgctcgatc
     6841 tatcaagagc tcgtcgaagc ggctgcatga tccacggggc gcaggtcctc ttgcggcccg
     6901 tggaggagcg ggaccatgtc ctgatccacg cctggcagaa cgaccccgag gtgtggtggc
     6961 tgatggacta cgaggggccg ttctcgatgc aggacgtcgt cgagagcgaa caacgggcgc
     7021 gcgcggaggg tcatccgtac gtgatcgagg tcgacgggac cccgatcggc cggatcgggc
     7081 tcaacgcctt ccgtcgccgc gaccgcatct gttccctcta cgtcctgatc ggagaacgct
     7141 cggcgtggga gcagggatac gggaccgatg ccgtgtcggc cctgttggac gaggcgttcg
     7201 atcgctacga cctccatcgc gtcgagctct ggtcgacggc cgacaacgag cgggcgatcc
     7261 acgtctacga gaagtgcggt ttcgtggtgg acggccggtt gccggagcgg tcctggaagg
     7321 gcggcgcgtg ggtcgatcgc gtcgtgatgt ccgtgactcg cgaggacttc gcagcgtcgg
     7381 ccacgggctc tgacctgcga cgacgcggtc actgaacgtc caccgaattg tcactctttg
     7441 tcctacgccg tctacctgca cgttcatacg ctgacctgcg cggatgcgga gttgaccgcc
     7501 cttggtgccg ccggtatctt tcgcctcgct ccaagcagtc gaggtgatcg acgcgagagg
     7561 gccggacgag cggccgggac atcgcgagat ggtccggacg cgagggaccc aggaggatcc
     7621 gcgaggatcc gagcgggccc ccgaccgccg gagaaggccg cgaggccgga cccggtgggc
     7681 ctcgcaggga acgccgcaag gcgggtcagg cggggggtcc gcgggagtcg gggcgccgcg
     7741 aggcgatccg gagcccgcga atggcggagg ggagccgcga ggcgaaccac cgtcggcggt
     7801 ccggcagccg cgcgaagaac gcggaggttg cttggggcac cagagcccga ggggctgaag
     7861 gtggctggga gcttcggttc ccagcgagcc agaggtccct cgggttctct ctttccttct
     7921 ctcgggggac cacgctcgcg ctggccgcgc tcgctccttg agccccccga accccctttc
     7981 cgctagcgtc tcgcggcatg gatgagtccc gccgtgatcg gatcctcgag gccgcgatgc
     8041 gacggttcgc cgatcgcggc taccgcggga cccgcgtcga ggacgtggcg aacgaggtgg
     8101 gggtggccaa ggggacagtc ttcctcgact tcggatccaa ggaggggctg ttcctcgagg
     8161 cgttcaagcg cgcggtctcc ctgctcccgg cgtggctgga cgctccggcc gacgtcgtcg
     8221 atcaggggtt ctgggccgtg ctcgactggt ggctccagcg ggcagaggac ctcgtggtgt
     8281 ccgaccccgt ccccaaccgc gtcgcgctga tcgcccggta cgacaccagc ctggacatcc
     8341 gccggccggt cgaccgcttc atgcggagcg aggacccgta cggcaccctc gagttcgtcg
     8401 agttcggggt gaagcgtggg gagctccgcg acgacgtgga cgccgagatg atcgcgtcga
     8461 ccctcgactg gatggccgag cggttccagg acgccctcgt gtccgaggac ctcgaccccg
     8521 gcctgatcca ccgccggccg gagcggcgcc cgatgcggat caaggagttc gtcgaggtgt
     8581 tgcgcggcgg catcgcaccg cagccggagt gaccgccctc tctgcggtac ggtgtgcgcc
     8641 gcgacctggc cggatccgcc ggccctcgaa cctgctgagg accagatgag cgaacgttcc
     8701 atcgcattcc tgggtggcgg ccgcatgggc gaagcgctcg tcgccggcct gctccgcgcg
     8761 ggcgcgcgga ccgaggacga ggtcctcgtg acctgccggc gcgaggagcg cgcgcaggag
     8821 ctcaccgagc gacacggcgt ggcaacgacg ctggacaacc cggaggcctg tcgcttcgca
     8881 ggcgtgctcg tgctgatggc gaagccgcag gacttcgagg tgctcctcca gcagatcgcc
     8941 gatcacgtga ccaccgaaca tctcgtggtc acgttcgcgg ccggcatccg gacctcgttc
     9001 gtggagcagc atctcgcacc cgacgtgccc gtcgtccgcg tgatgtcgaa cgtcccggtg
     9061 atggtggacg aggcgatgtc ggtgatcgcg gcgggccgcc acgccgatga caagcacctc
     9121 gccgtggcgg aggagctgct ggccgccgtg gggaaagtgc tccggctcga ggagcgctac
     9181 ctcgacgccg tcacggcaac gagcggctcc gggcccgcct acttcttcct gctcgccgag
     9241 gcgatgatcg aggcatgcat cctgctgggc ctgtcgcgtg atgtggcgag tcagctgatc
     9301 atccagacga tgctcggttc ggccaagatg ctccgcgaca ccggcacgca cccggtggag
     9361 ctgcgtgaga tggtgacgtc gccgggtggg accacgatcg aggcgatccg ccacctggag
     9421 gaggccggcg tgcgcgccgc cttcctgaac gcgatcgatg ccgccaggcg ccgcagtgag
     9481 gagctcgcgc gcggcggcga cgaggacgac tgaggatccg agggagaaga cgacatggga
     9541 cgaggagatc tggcaaaggt gcgcgacgca cgcgaccggg ggcggaagaa gaaggagcgc
     9601 gagaagcgcc aggccgcgga gcgcgccgcc gcgcggaagg aagccaagaa gaagaagtag
     9661 cgcctacggc cgcgcgccca ggcggttcct gagggttccg atcccttcgg ccgcgagctc
     9721 gacaagggcg cccggccaca gcctgcgatc cggatcgtcg tcgcgcaggg ggaagggccc
     9781 gagtgcgaag gcgtcccctc gttccaacgt ggtccctcga gaggcggccg cgatcaacct
     9841 gaacaggctc cgggccgacc cgttcaggtt ccccttcgcc agctcgccgc cgtccacctt
     9901 ggcctgcagg aacatcgcct ggggatcgca ctcgtccacg gtgaccacgc acgggccgat
     9961 cgcgatgggg acgccctcgg aggtgccgac ggggtcgccg gacgcatcct tggccaccca
    10021 gtcgctcacg agcgtgtagc cgaagatgtg ctccgcaacg gcgtccgtct cgacgtcctt
    10081 cgtctcgatc gccagcaccg ccgccacctt cggctcgtaa tcgagccagg cggcgccggc
    10141 cggccacgcg atgtcctgct cgggtccggc gatccgtcgc tcgaggcctt cgcccccctc
    10201 accgcgctcc ggaccaccgg ggaggaggag cgaccggggg aagagcggcg gcagcacgcg
    10261 ggcctgcctc acgacgtggt ccttcacgtc gtcgcgggcg agggcggcgc gggcggcatc
    10321 gagcaccgtc ccccggctgc tctgcacgaa cgcctcgagg gtggtgggaa aggccgggtg
    10381 cccgactgcg tcgggcagat cgaccaccct cccatcgagg atcgccccca gtcgccggtg
    10441 ccccctccgg tcgtaggtca cgagcctcat ctcagggagc ccgagcatac gcccggcctc
    10501 cgacggaatc tcctgttccg cccggaaatc gcggtcatcg gaccggtgcc cgcccgtgct
    10561 cgcccggggc gcgcggcgat agcatccttt ccgccgcaga acgagcgccg agacgcaaag
    10621 gagtcgcacg atggccgaga agttccggat cacctacgcc accctgtccg cggacaacga
    10681 ggacctgcag cgcaactacg acgaggccgt ggagcgcgcc cgagccgagc tcggcaagga
    10741 atggccgttc gtcgtgaacg gcgaggagcg ctggtccgag gagaagtacg aggagccctc
    10801 gcctatcgac gaggacctga tcatcggccg cttctcgcag gccacgccgc aggacgtcga
    10861 cgacgccgtc gaggccgcga aggcgttctc gctcgagtgg gagcggatcg gatggcaaga
    10921 gcgcgtccgg atcctgcgca acgtggccga cgtgatggag gaccggctgt tcgacctggc
    10981 tgccctgatg gccttcgagg tcgggaagag ccggctcgag gcgctcggcg acgtccagga
    11041 gaccgcggag ctgatccgct ggaactgcga cgaggtggag aagcacgagg ggttccggac
    11101 cccgatgtcg ggactcggct cgcccgggga ctactacgac gtgatgcgcc cctacggcgt
    11161 ctgggcggtg atcagcccct tcaacttccc gttcgcgctg gcgggaggtc cctcctcggg
    11221 tgcattggtc accgggaacg cggtggtgct gaagccctcg aaccagggcg ccctgatggg
    11281 ctacaagctc tacgagtgct accgcgacgg cggtgtgccg ccgggcgcgt tccatctggt
    11341 cgtcggacga ggcgcgctcg cgggtgaggc gctctggcgc caccgcgacg tgaacgggat
    11401 cacgttcacc ggctcctacg cggttgggat ggacatcctg aagaacttcg tccaggacgt
    11461 gcccaagccg gtgatcgccg agatgggcgg taagaacccc gcgatcgtca cgaagcatgc
    11521 ggatctcgac gtcgcgaccg acggcgtgct ccgctcggcg ttcggcttcg gcgggcagaa
    11581 gtgttcggcg aactcgcgcg tctacgtcga gcgcgaggtc tacgacgact tcgtgcggct
    11641 gctcaaggag aagaccgaga agatcaaggt cggcaacccg atcgagcgcg acgtcttcct
    11701 cgggccggtg gtgaaccacg aggcgctcga tacgttcgag caggcagccg gcgaggccaa
    11761 gaagaacggc acggtgatca cgggtggcga gcggatcacg gcgggcgatc tcggccgcgg
    11821 attgttcgtg cagccgacga tcgtggaggc gcccgaagac agctggctgt ggaagaagga
    11881 gctgttcgtg ccgttcttgg ccgttgcccc gttcgacgag ctggacgacg cgatcgacaa
    11941 ggcgaacgac accgagtacg ggctgacggc cggcttcttc agcaaggacc ggcacgagat
    12001 cgaggagtgg ctcgacaaga tccaggcggg ggtcgtgtac gtgaaccggc gggcgggggc
    12061 gacgaccggc gcctggccgg gtgtgcagcc gttcggcggc tggaaagggt cggggacgac
    12121 cggcaaggcc ggcggcgggc cgtactacgt cctgcagtac ctccgagagc agtcccggac
    12181 ggtgatcgag gagtgagcct cgaggacctc gagctcgagg gcctggcgaa cgagtccgct
    12241 ccacggctga tcaccgagat cccgggccct cgcgctcagg aggtcgtgga gcgagaccgg
    12301 gcgacgacgt ccccgtcgct cccacgcgcg tatccgttcg ctccgaagcg agcgtcgggc
    12361 agcgtcgtcg aggacgtcga cgggaacctc ttcctcgacc tcaacgcggg gatcgccgtg
    12421 acgtcgacgg gtcattgcca cccgaccgtc gtcgaggcga tccagcggca ggccgccgag
    12481 ctgatccatt actcggcatc ggatttcttc ctgcccatct actccgagct caccgaacgg
    12541 ctggaccaga tcgccccgat gtcgggacgg gtcagatcgt tcctgacgaa cagcgggacg
    12601 gaggccgtcg aggcggcgat gaagctggcc cgccatcaca cgggccggca gtacctcctg
    12661 gccttctacg gctccttcca cggcaggtcg tacgggtccg tctcgatgac cgcctcgaag
    12721 tcctcctacc gtgccgggtt cggacccctg ctgcccggcg tgttacacgc cttctacggc
    12781 gacggcaggg acatcggctt cgcacccggc tccggttcgg aggggttccc cacgatcgac
    12841 tacatcgagg gattcctcat gaagaagatc gtggcgccga ccgaggtggc ggcgatcgtg
    12901 gtcgagccgg tgctcggcga gggcggctac atcgtccccc ccgacggatg gctgcaggcg
    12961 ctccgcgagc tgtgcgaccg tcacgggatc atgttggtgt gcgacgaggt gcagtccggc
    13021 atgggccgca ccgggaagat gtgggccgtc gagcacttcg acgtggagcc cgacatcctc
    13081 ctcgcgggca aaggcatcgc gagcggcatg ccgctcgggg cgatgatcgc gcgggacgac
    13141 cggatgacgt ggacgatcgg gacgcacgga tcgacctacg gagggaacgc cgtgtcgtgt
    13201 gccgcggcgc tggccacgat cggcgtgatc caggaggagc tgctgctgga gaacgcggcc
    13261 aaggtcgggg atcagctgat gaacgggctg cgcgacatcc agagccgaca cgacacgatc
    13321 cgcgaggtcc gtggcctcgg gttgatgatc ggcgtggagt tccccgatca cgggaccgcg
    13381 gccgcggtcg aacgtggagc gttcgagcgc ggcctcctga tgctcgggtg cggtgagtcg
    13441 gcgatccgcc tgtccccgcc gctcgtgctc cgcgaggacc aggcgcgcgt cggcctcgat
    13501 ctcttcgaag aggtcgtcgc ggcggccgct ccgggcgtcg gggctgttcc cgactccgga
    13561 gcctgacgga ggccgatccg atgccgctcg ggctggacaa ggtcacgttc gactgcgccg
    13621 atacgatgcg cgtcgcgagg ttctgggcgg aggcgctcgg gttcgacctc gacccggagt
    13681 ccgagccgga cggcgcgctc atccgcgacc cctccggacg ctccgacggc atctacttcc
    13741 agcccgtccc cgagtcgaag atcgcgaaga atcgcctcca cctcgacctt cgccccaccg
    13801 gatccatggc ggccgaggtc gagcgcctcg aggggctcgg cgcgacggtc gtcgggcgcg
    13861 tcgacgaggg gggcagcttc tggacggtga tgcaggatcc cgagggcaac gagctgtgcg
    13921 tgctccgcgg gcccgaggac ggttggaacg cgggatgacc tccaaggtcg cgacgatgca
    13981 tgaggcagtc gcggacctcg tccgcgacgg cgacaccgtc gcgatcgagg gcttcacgca
    14041 cctgatctgc ttcgcggccg ggcacgagat catccgtcag cgcaagcgcg accttacgct
    14101 cgcccggatg acgccggacg tgatctacga ccagatgatc ggcgccgggg tcgcgaggaa
    14161 gctgatcttc tcctggctcg gcaatccggg cgtcggctcg ctccatgccg tgcggcgccg
    14221 gatcgaggac cacgacccgg cacccctcga gatcgaggag tattcgcatt tcggcatggt
    14281 ctgccggtac gtggcgggcg cctcgaacct gcccttcttc ccgatccgca gctactacga
    14341 gtccgacatc ccgggggtga acccgaagat cgttccgatg acctccccgt acgaggacgg
    14401 ctccgaggtc tacgtcgtgc ctcccctgaa gcccgacgtc acggtcgttc acgcgcagcg
    14461 cgccgaccgc gacgggaacg cccagatctg gggactgctc ggctgccaga aggaggcggc
    14521 gttcgcggcc gaacgggtga tcgtcgtggt ggaggagctc gtcgagggtg acgtgatccg
    14581 tcgcgatccc aacaggacgg tcatccccgg catcgtcgtg gatgccgtgg tggaggagcc
    14641 gttcgcctgc catccctcgt tcgcgcaggg ctattacgat cgcgacaacg agttctacct
    14701 ggcgtgggat gcgatcgccc gcgaccccga gtcgctggac gcctggctcc aggagtgggt
    14761 ttacggtttg gcctcgcacg cggactacgt cgagaggtgg ggcgccgagc actgggcggc
    14821 gttgaagccc ggcccggcgc tgtccggcga ggtcgactac gggagctacg cgtgagtgtg
    14881 ccgtcgagcg acgacgacgg tagggcgcga tacagcaaga acgagctgat gatcgcggcg
    14941 agcgcccggc tgctcgccgg tgtcgagaac tgcttcgtcg gcgtgggcct gccgaacatc
    15001 gtctgcaacc tcgcgcagcg cacggtcgcc ccggagctcc agctcgtcta cgaatcgggc
    15061 gtattcggcg cccgcccccg tcggttgccg ctgtcgatcg gcgacccgac cctcgcaaca
    15121 ggatccacgg cggtgacgtc gatgttcgag ctcttcgcgt tctacctgca ggcgggcctg
    15181 atcgacgtcg cgttcctcgg cggcgcgcag atcgaccggt tcggcaacct caacacgacg
    15241 gtgatcggcg actacgcaca accgaaggtg cgcctgcccg gctcaggagg cgcctgcgag
    15301 atcgcgatcc acgcccggaa gatcctcgtc atcatgcgcc aggcggaacg atccttcgtg
    15361 cagaagctcc acttccgaac gtcgccgggg cacagcgggg atccggcgca cgaccgtgag
    15421 cgcggctggt cggggagcgg ccccacgaac gtcgtgaccg acctcgcgac ctacgcgttc
    15481 gacgtggaga cgggcgagat gaccttgcac acgctgcacc cgggcgtgcg tctcgaggat
    15541 gtgcgggcga acatggcgtg ggagcctcgc gtggcgcggg acatcgccga gacgccgccg
    15601 cccacctccg aggagctccg gctcctcagg gaagagctcg accccggccg gatctacacg
    15661 aagtagcctg accgacgtga gccacgtgtt ctcgcggtcg agcctcgacc tgccgacggc
    15721 ggtatccgcg gacggcgcct ggatcaccga cgccgatggc acgcgctacc tggacggcgc
    15781 cggcggcgcg atcgtcgtga acgtcggcca cggcgtggca tcggtgatcg ccgcgatcgg
    15841 cgagcagctg gaacgaacgc agtacgtcca tccgacggcg ttcacgaccc gggcggtcga
    15901 ggcctacgcg gaggaggtgg ccggcatcct gccgatggac gacgcccgga tctacccggt
    15961 ctcgggcggc tccgaggccg tggagacggc cttgaagatg gctcgcgcct accacctcgc
    16021 gcagggccgg ggcgggcgct cgatcgtgat cggtcggcgg ggctcgtacc acgggaacac
    16081 gctcggcgcc ctcgacgcct ccggcaaggc tccgctccgc aagccctata cgccctggct
    16141 cggccggttc ctccacgtct cggccgccta cgagttccgc tgcgagaacc cgcagcatcc
    16201 gcacggatgc ggggcgtggc aggccgcgga gttggagaag ctgatcgcct cctacggcga
    16261 ggacacggtc gcggcgttca tcggcgagcc gatctccggt gccacgctcg gcgcggcggt
    16321 cccgaccgac gactactggc cggcgatcgc cgaggtctgc cgagctcacg acgtggtcct
    16381 gatcgccgac gaggtgatga ccggcttcgg ccggacgggg aggtggttcg ggatggacca
    16441 ttgggacatc cggccggaca tcctcacggc ggggaagggg accacgagcg gctacgtccc
    16501 gttcggtttc gccgcggcat cgggccagat ccatgaggcc atcgccggcg gcccggggtt
    16561 cgtccacggg ttcacctggt cccacaacgc cctcggcgcc gccgcgggtc tcgcggtgtt
    16621 gggcgagttg aaggccggtc tcatcgatcg cagccgagag ctcggcgcgc gactcctgga
    16681 cggactccgg agcgagctcg cgggtgcgcc cgcggtcggg gacgtccgcg gcctcggcct
    16741 gctgatcggg atcgagctcg tgcgggatcg ggagacgaac gagccgttcc tgcgcgccga
    16801 cggggtctcc gaacgcgtct tgcaggcggc ccgcgagtcc gggctgctgc tctactcctc
    16861 gaccgggcac gtcgacgggg cgaacggcga tctcgtgctg ctcgggccgc cgctctcgct
    16921 ctccgacgac gaggcctctc tcctgctcga gcggacggtt gcggccgttc tctccgtccc
    16981 gtgagcggcg acgtcgctct ggtccggtgc ccggaggcgc gcatctacga ccacggtccc
    17041 ggtcatccgc tccggccgga ccgcgtgctc ctgacgtggg atctgatcgc cgccacgggc
    17101 ctggaccagc gcgagggggt ggaggtgctc aactgcgagc cggcgtccga cgacaccctc
    17161 cggctggtcc atacgaagga gtacctcgag gcgacgaagg ccgcggggag cggggtggag
    17221 ggcgactggt cccggttcgg ctacggcccg ggagacaacc cgatcttcga ccggatgcac
    17281 gaggccggcg ccctggtcgc gggtgcctcc gtggaggccg tccgagccgt ctggagcggc
    17341 gatgtgcttc acgcgttcaa cgctgcgggc ggcctccatc acgcgatgcc cgcccgtgcg
    17401 agcggcttct gtgtctacga cgacccggca gtcgcgatcg cgtggctcct ccgtgaggga
    17461 gccgaaaggg tcgcgtacgt ggacgtcgac gtccaccacg gcgacggcgt ccagtcgatc
    17521 ttctgggacg agccccgggt gctgacgatc tcgatccacg agtacgtccc cgggatgttc
    17581 ttccccggca ccggcgcggc gtctgagcgt ggtggcccgg gcgcgaaggg gtccgcgatc
    17641 aacgtgccgc ttccgccgga gacgggtgac gacgcgtggc tcgacgcgtt ccgggcggtc
    17701 gttccgcccg ccgtcgaacg gttcgctccg gacgtgctgg tgacccagct cgggtgcgac
    17761 acgcacgcca ccgatccgct cgccgagctc cggctgacga cgcgcgccta ccgcggatgc
    17821 gcccaggagc tccacgcgct ggcgcaccgg accgccgctg gtcgctgggt cgcgaccggc
    17881 ggcggcgggt accagtgggc gcgcgtcgtc ccgcgcgcct ggaccctcgc cttcgccgag
    17941 atggccggcg cgctcgccga cctgcccgat cagctccccg aggggttcat cgagcacgcg
    18001 gagcggcggt tgggtgacga ggttccggcg acgttctcgg agcccgacct cggtcccggc
    18061 cgcggcgatg acgacgcgag ggcggccgcc gacgaggtcc ggcgcctcgt cctggggcca
    18121 tgagcgcgcg gacgaagctc gtctgcacgc tcgggccggc gagcgcgacg cccaagatgg
    18181 ttcggggcct cgtcgaagcg ggcgcttcgg tcttccggat caactgctcg cacggcaccc
    18241 gggaagatca cgcgcggagc gtctcgatcg tgcgcgaagt ggagggggcg accggccgct
    18301 cactgtcggt cctcgccgac ctcccggggc cgaaggtccg cctcgcggac gtgcaccccg
    18361 atcccttcgt gttccggccc ggccagccgt tcgagatccg acccgacggg cccggcgacg
    18421 cgacgggggc cgccacgtcc tacgcggggc tcgcggacga cctccgcgtc ggcgaccgca
    18481 tcctgctcgc ggacggcgcc gtcgagctca cggtcaccgg gaccgagggc gaccgggtgc
    18541 gaaccgagtg cgtgcgcgcc ggcacggtcc gtagccacca aggggtgaac gtgcccgccg
    18601 agcgactgag cttgccgccg gtgacggaga aggaccggga gatgctgcgc cacgtgctcg
    18661 acctcggcgt cgatctcgtc gcgcagtcct tcgtcagggc tccctcggac gtcgaggagc
    18721 tccgcgcggc gatgggggat cggcccgtcc cgatcgtggc caagatcgag acccgcccgg
    18781 ccgtggaagg cttcgagcgg atcctcgagg tctcggacgc ggcgatggtc gctcgtggcg
    18841 acctcggggt cgagctgccg atggaggaga tccccctcgt gcagaagcga ctgctcaggg
    18901 cagccagggt cgccgatcga cccgtgatcg tcgccacgca gatgctggaa tcgatgatcc
    18961 ggtcgccgcg accgacccga gcggaggcga acgacgtggc atccgcggtc ctggacggcg
    19021 ccgacgcgat catgctctcg ggtgagaccg cgatcggcga gttcccgttc gaggcggcgt
    19081 cggtcgcgac ccggatcgcg cgagccgtcg agtcccaggg cgccgagtac cgcgccgcgc
    19141 agccaccgtg tctgcactcc ggggaggcgg cagcggtggc ccacgccgcc gcgtcgatcg
    19201 tccagggcga ctccgacgcg gtcgcgatca cctgttacac gaccacgggc cggacggcgg
    19261 cgttgctctc cgcggagcgg ccccgcgtgc cgatccacgc gttcatcccg gatgtcagcg
    19321 ttcgccgggc caacgggctg ctctgggggg tggaggcgca cggcgcggag cggccggacg
    19381 atacggacca gatgatctcg atgatggacg agcggctccg cgctgccggc ctcgccgccg
    19441 acggggacac cgtggtcatg gcggcgtcga tgcccgcggg tcggaccacc acgaacatgc
    19501 tcaaggtcca tcaggtcggc tcgctcgagg cctgagcagg tccgagagca ggctcgagag
    19561 ccggccaggg agcaagagag cgcaagcagg cggttgtgcc gggttcggag gacgcctagc
    19621 gtgaacgagc gatggcgctc cgctctccga acgagatccc cggccgcccc tcgggcgtgc
    19681 tcgtgccgac ctacagcgac aacgagcatc cgggcacgaa ggcggagcac cggcgcctcg
    19741 cggtcgaatg gcgacgtcgg cacccgcagt ggtggaagcg gctgctccgc ctgaggtcag
    19801 tcccccccgc cgactgaatc ctctccctcg ttgcggggct cgcccacgtc cggcgcgccg
    19861 atcccttcga gcagctcttg gagtaggaag ccgaggaact gatagatgcg gtaggtctcg
    19921 tgtcgaggat cgtcccccgg gaagtcctca tccgtcgctt cctccgtgat ctcgatccgc
    19981 gtaccgagca cgagtcgcag atcgttcacc acgccgatcc acgcgaggag ctcgtcctcg
    20041 gtcagcgccg acgcgttcgc ggtgcgctcc acgacatcga gggcctccag cttccccgcc
    20101 cgcggggcgg cgccgaggtt cgtctcgaac tcgaggttct gcagcgggtc gtcggggtac
    20161 gccggcggga agagccggga cacggccggg tcgctcgatg gatcctccgc gtcgagcagt
    20221 gcgcgggact gccggcagag ctcgatgagg aggccgcgca caccccggct gagacgcacg
    20281 gcgaaccgat cgccctcctt ggagaacgcg ggctccatca cgcgtcccgc tgcagcgtcg
    20341 cccacagccc gtgggcgtgg aggcgggcga cgtcggactc cgccttctcc cggggcccgg
    20401 aggagaccac ggcgcgtccc ttcgtgtgga catcccacat caggcgctcg gccttctcgc
    20461 gcgggtaccc gaacaggcgc atgaacaccc acgtgacgta gcccatcaga ttgatcgggt
    20521 cgttccagac gatcacgatc cacggcaggt cgggttcgtc gacctcgtcg acgtgcggct
    20581 gctggacgcg ccccggtctc gtcgtggtcg tccggagcga cgtcatcggc tcgtggatcg
    20641 acgggcccga cggatcctcc ggatccccca tcggtagagc ggcggtgtgg cgacgcggaa
    20701 gagctggaag gggctcagcc atcgcgggac ggagtactcg ggcgcgatgt cttcacggac
    20761 caccttcacg atcgcatccg tcaccgtccg catcttgagc accgcccagt ccggaaggtc
    20821 gcgctgcggg aacccctccg tgtcgacgaa cccggggttg accgacgtca cgtgcacgtt
    20881 gcgtccttcg gtctcgtaat ccatcgcttc ggagaacgcg acgacggcgt gcttcgtcgc
    20941 gccgtagatc gcgttgccgg gcgtcgcgaa gcgcccggcc agcgacccga tgttgacgat
    21001 gtgacccttg ccgcgtgcga gcatgctcgg gaggaacgcc cgcgtcccga acagcacgcc
    21061 gaggacgttc agtcggacgt ggtcctcgat ccgatcgtag tcgagcttcg tgaactcgcc
    21121 tccgccgcgc atccccgcgc tgttgacgag gacgtccgcc ggtccgtaca cctcttccgt
    21181 caccacgcgg agcgtcgtga gctgctccgg ctcggtgacg tcgcaggtgg cggccagggc
    21241 tttcccgccc gatcgctcga tccgctcgac gagcgcatcc agccgcgcgg tccggcgcgc
    21301 ggcgagcacc acgcgcgcac cgcgatccgc gaacgcgagc gccgccgcct cgccgatgcc
    21361 ggacgacgcc ccggcgacca cgaccacagc tccctcgatg cgcaggacga accccctcgt
    21421 acgatggtgg tgccttggac gttatcggag cccacgccgg tcgtctgccg gtgctcgacg
    21481 agcgctggga gccgcggccg tatccacggg aggagttgga gcgcaccctc gtggccggcg
    21541 gggtggcggg acgggcgggt catccgcatc acaacgtatt cgggaacatc cggaaactcg
    21601 tcgagcacga tcccgacaag ctcctcggtc tttcgaacgt gcccgaaccc ttcggcttcg
    21661 aggacatcgt gcggatcgtg ggcgaggcgt ccggcgtgcc gatcgacgcg ctggcgaccg
    21721 agggatcgcc cgacatcgcc gcggggtccg tgatggacgc gtgcgaggcc gtgggggagc
    21781 gcctggcgat cgccgcgcgc cgtcgcgagc gggtgttgtt cgccacaggg catcccggag
    21841 acctcgaccc gctgtacggc gcgatcgccg agctcgcgac ggcgcgcggc gcggcgatcg
    21901 tcagacccgc cgaaggcgag acctggttcg acgatcacgc gggcacggag tggacgatcg
    21961 ggtaccacgg cgccgtcggc atgatcaccg acggggagcg gccgaggcac acccactggc
    22021 cggaggcgat gcgtcgcatg ctggccgacg aacggccgga tctcgtgatg gccgatcatg
    22081 gactcgcggg agccgcgata gaagccggcg tcgagacgct ctcgatcgcc gatgtgaacg
    22141 atcccgcgct gatcgtcgcc aaggctcagg gccggaccga gatcgtggtc gtcctcgacg
    22201 atcatgttcc gcccgagaac tactggccgt gcttccaggc ggtcgcgggc cgcttcgatc
    22261 cgagctagtc gctgcgcgct ccgcgcgtgt ccggctcccg gacgtcgatg atctcgtcct
    22321 cggccgggcc gtcatcgtcg tgtccggtgc cggcgagtga cttcaacttg taggagacac
    22381 cttggcgcgt gatgtgcagc gcgtcgccga tgtcctcgag cgacgcgccg agctcgcgag
    22441 cacgcaacat cgcctcggct tctacggcat cgaggtcggt gcgaagctcg cggagcgcct
    22501 gcagagcgac caaggggaac gcgttcgcgt gctcgagtct gcgcagcagc gcgcgcctgg
    22561 tgtcggtcac gcggtagagc ataggagcgg tgggcgcaag tggcaaggat ccctcgatgc
    22621 aaaatgcctt gcgaggggtc ccccgccggg tcttgccaga agggcctcat tcaagcacga
    22681 acctaaggga gccgtggtca agaacccttg gcgtaggacg ttcggctgta ttgaccgctg
    22741 tcccccatct cacctaggct gcacttcacc ccaactgcct acagcagtag cgggaggatg
    22801 ggataccggt cgggcgcgcc cggaggggct ctccggagcc gggcgcgccg acaggttcaa
    22861 ccgagaggac gggtggatgc gccgaagcaa agcagattca acgggttcga ggacgcagtt
    22921 cggtgatcgg tggtccttcg ggccgcgtcg tgcccgaggg gtccgtcgcc accactcgtc
    22981 ggtgctctct gcggcactca ccctgttcac ggttctgggc atgaccggag cccttgcgga
    23041 caacctccag aacgatgtca cggccggtgg caacgacacg atcacggctg gtggctcgac
    23101 gaccatcacc taccgcctga tcggcaacaa cgcaccctcc ggcgatgtca gtggctgcaa
    23161 tgtcgacgcc tccaacccag caaccgtcac gatctctctt cccggcgccg tcacggagag
    23221 cccgagctcc ttccagttca ctgcctgcgg caacgccggc gcgaagatcg ttacctacgg
    23281 ttcgtcgacg gggggtgact acctgatcaa cgtcagtatc agcggcggga agccgggatc
    23341 gctgtacaac gatcaagcag atctcaccct gcatgtgaat gccgctccag ttgtcgacac
    23401 cgatggcgac ggtgtcgtgg actccgccga caactgtctt accgtcgcca acgccggcca
    23461 ggaggattct gatggcgacg gtcttggtga tgtttgtgat ccggataccg acggagatgg
    23521 cgttgcgaac gcctcagaca actgccccgg cgttgccaac cccggacagg ccgataccga
    23581 cggcgacggc ctcggagatg cctgtgaccc actcaccgac tccgacggcg atggtatcgg
    23641 ggatagcgtc gacaactgcc cgaacgtcgc ggacgctgat caagccgatg cggacggtga
    23701 cggactcggg gacgcctgcg atccggacac ggacggagac ggtgtcctga acgtcgcgga
    23761 caactgccca gccgtcccca acgcagatca ggctgatgcg gacggtgacg gagcagggga
    23821 cgcgtgcgac cccgatacgg acggagacgg tgtcctgaac ggcgtggaca gctgccccac
    23881 ggttgcgaac gccgaccagg tcgatgtcga tagtgatggc ctgggggatg cgtgtgactc
    23941 cgatcgagac ggcgacggtg tgccgaacgc gaccgacaac tgccccgatg acccgaaccc
    24001 tggacaggcg gacgccgacg gcgatggcct cgggaacctt tgcgatccga acgccttcgc
    24061 acctcagatc gtcgacgatg ccgacgacgt gaacggtttc gaagcggtcg agctcacgaa
    24121 ctcgggctcg ttctcagacg gtgactcgga cacacttgcg atcacgaagg tcagtggggc
    24181 cggcgccctc gttgacgacg gggacgggac ttgggagtgg tcgttcacgc caggcgacga
    24241 tgcaagcggt tccgtcgtcg tacaggcgtc ggacggcgac catgccccca cgaccgactc
    24301 attcgattgg acagcgacca acagcccgcc ctcgatcagt gtcgtttcga acaacggccc
    24361 gatcaacgag gccgcctcgg catcgatcac cgtcaccgcg acggacccgg ctgggtcgaa
    24421 cgatccgctc gcgtactcgt tcgactgcga ccacgatggg acgttcggag cagctggcag
    24481 ctcgaaccag ggctcctgct cgttcggtga ggatggcacc tacaccgtgg gcgtgaaggt
    24541 gaccgacggc gacggcgggg aggacacgga ctcgacgagc gtcagcgttc tcaacgtcac
    24601 gcccacgctg gattccctca cgctcacgcc ttcctcgatc aacgagacgg gctcgaccac
    24661 gctcgccgcc gccgtctcgg accctggggc cgatgactcg gaactttcct actcgatcag
    24721 ctggggcgat ggctcggcgg atacgaccgg ttcgacgtcg accggtgcga tctccgagag
    24781 ccatgcattc gccgacgacg acgccgacga ctcctacggc gtgacgctca cggttgagga
    24841 ctccgatggc gatgccgact ccgataccgc caacgtcacg gtgtccaatc tcccgccctc
    24901 gatcagtgtc gtgtcgaaca gcggcccgat caacgaggga tcgtctgcga cgatcaccat
    24961 caccgcgacc gacccggctg ggtcgaacga tcccctcgcg tactcgttcg actgcgacaa
    25021 cgacgggacg ttcggagcag ctggcagctc gaaccagggc tcctgctcgt tcgcacagga
    25081 tggcacctac accgtggggg tgaaggtgac cgacggcgac ggcgggctcg acacggactc
    25141 gacgggcgtg agcgtcctga acgtcgcgcc gagcgtgagt ctcacgctca cgtccgcctc
    25201 tgggacctgc gctgcgaaca ccgcgagctt gaacctctcg ttcacggacc cgggggcaga
    25261 tgcaccttgg gatgccaccc tcaactgggg cgacgggagt actgaggttc tcagcctcgc
    25321 caccccgggg acgtcgactc agctccaccc gtacgcgtcg gcggggcagt acacgatctc
    25381 ggtgaccgtc aaggacgacg acgtcgcctc tgcgtcggca acgatcgagt tcacgctgaa
    25441 ctacgacctc agcaccatcc tgcagccgat caacgacacg cggaacgggc agccgacgag
    25501 cctcttcaag tacggcagca cgatcccggt gaaggtggtc atcaccgact gcgatgggtc
    25561 gctcccgacc gatctcgcgc cgaaggtgac ctggagacag ggtctcaacg cgaccccgac
    25621 ggggatcgat gaggccgttc cgacgagtca ggctgacctc gggaacacga tgcgcttctc
    25681 gggcggtcag tacgtgctgc agctcaactc gaagaacacg acgagcgatc cgtcatgcgg
    25741 cgtcacgatc tgggtgacga tcccgacgac tggccaaacc gtccaggcga acatcggttt
    25801 caagaagtag tcgcactcag ggggttggtg ggcatcccct gtgaggagca gcaggagacg
    25861 gggccagttc tggcccctcc ctctgggttt tcgaggtgtt tcgcgcgcct cgatccagtc
    25921 aacctgtgcg tgccctactt tgaaggcatg ctccttgaaa gcttgcgccg caggctgttc
    25981 ctgatgctcg cggatcgtca ggactatcca gctgttgggg ggctggggta ccatcgatga
    26041 acggaatggt gatgccagaa cccttcaccg aggctcggct gctgaccgcg aacgaggtcg
    26101 cggatctgat gcgcgtgtcc aggatgaccg tctaccgact gatcaagacc ggcgagatgc
    26161 cctcgctccg cgtgggcagg ggctaccgct tccgcgagga agacgtccac gactacctga
    26221 gggggcgcta caccgaggcc ggctgacacc ggcaccgtgc agggctccga cacgctcgag
    26281 gccgaacctc cgcgaagggg cggagggcgc gatgaacgag cacgaccggc tcgtcgaccg
    26341 ggccgcgcga gaggcgcggg ccgtgctgtt cgtgggcggg ctcgattccg ggaagagctc
    26401 gatggcgcgc gccacagccg cgtacgcgct cacgctcggt cgctccgtcg cctacctcga
    26461 cgccgacgtc tcccagaaga cggtgggccc gcccggcacg gtcgggctca agcacatccg
    26521 ggagcccgac gacctcacgc acgatcgcat ggcccaagcc gacgcgatcg gcttcgtggg
    26581 atcgacctcg ccgcaggacc acctgctgcc cctcgtgggt gcgctctcgc gcctgcgcga
    26641 gcgggcacac gacgagggcg cggagctcgt cgtcgtcgac acgggtgggg aggtctcggg
    26701 gatccgaggg cagctcgtga agtactacaa ggtggacacg ctcgagcccg atctggtggt
    26761 cgcgctgcag cgaggggagg agctcgagcc gatcgtgggc gtgatggagc gcttcttcgg
    26821 tgtcgaggtc gtccgcctgg ggatccatcc cgcggtggtc cccacgacgg tggacgagcg
    26881 gatggcgcag cgcgaggaat cgatgcgccg ctacttcgcg cagccgctcc agcgcttccg
    26941 cgtccgcccg acggtgttca tgccgagcct gccgccgctg ttcgacctct cgcaactcga
    27001 ccggctgttg gtgggcctgt cggacggcga ggggggcttc acgggcgtcg gctacctcga
    27061 gccggtctcc ggcaacggcg gtctccggct gatctcaccc gtggcagagg cgcccaaagc
    27121 gcttagactg ggctccgttc gtctggagga gagcttccga gcgaagcgga tcgacctccg
    27181 gaacctcttc ggaacagaat gaaaggcgtg gttcgttgcc gtccctcgtg aagaagcgcc
    27241 ggaagaagat gcgcaagaag aagcacaaga agctgctcaa gcgcacccgg caccagcgcc
    27301 tgaagctggg caagtagcgg cgggaggctc ggcgtgc
//
LOCUS       DASUYD010000063         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_692338, whole genome shotgun sequence.
ACCESSION   DASUYD010000063 DASUYD010000000
VERSION     DASUYD010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692338"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="VF195_03515"
     CDS             complement(<1..865)
                     /locus_tag="VF195_03515"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6579921.1"
                     /translation="MRETSATTGRARREADRGPGRPRRRWLQRLAAGVVVVALLVVGV
                     YAWAWASLDRSSIARAMWWREADVGDQYRFPARTIAAGDEASPLPAGDEIDPPAPTSD
                     TEDDRAFDEFLRGTGTLGFVVLDDDQLVYERYFGADRQTRQTSFSVAKSFLSTLVGIA
                     IEEGLIGNVTDPVTEYLPELSERDPRFEQITLRHLLTMTSGIRYEEQSLPLPWGDDVD
                     TYYGTDLRDLGLAETEIVSPPGGGWLYNNYHPLLLGLVLERATGMSVSEYMATKLWQP
                     LGAEANATWSLD"
     gene            complement(955..1284)
                     /locus_tag="VF195_03520"
     CDS             complement(955..1284)
                     /locus_tag="VF195_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6579922.1"
                     /translation="MADPQREMAEDPTDRREEARRWVRRKRIFYTVVGIYLALSVMWF
                     AIDLLDDSSGYWFYWPMLGTGLGVLVTGVVLGGLGGLFGKDWERRQVQRYLGEQGDQE
                     TRPDAKS"
     gene            complement(1339..1830)
                     /locus_tag="VF195_03525"
     CDS             complement(1339..1830)
                     /locus_tag="VF195_03525"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6579923.1"
                     /translation="MQIENETHDLVVTRTFDAPQERVWRAWSDADQVMRWWGPQGFTS
                     PMCRMDFREGGTTVVCMRSAEDWELYNTWTYRSIDPMDRIEFVQGFAKEDGTRVAPAE
                     LGLPPGIPEEVRHVVTLSPIDSATTRLTVREYGYPNPEIVEISRAGMEQVLDKMAATV
                     AGA"
     gene            complement(1881..2351)
                     /locus_tag="VF195_03530"
     CDS             complement(1881..2351)
                     /locus_tag="VF195_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX6579924.1"
                     /translation="MADDTQAQLPTPDPALRRLDRLVGRWTMEGNLLGSDERNIKGET
                     TFRWLPGGFFLEQRAHIDFMGLQIDALELIGYDPETDSFPSTVFSGFSPEPLPYRWDV
                     LGDAVTIAVSYGELNATFTGSWREEGTFSGGWRPNPGADETVNVPYDIGGHRLT"
     gene            complement(2553..3218)
                     /locus_tag="VF195_03535"
     CDS             complement(2553..3218)
                     /locus_tag="VF195_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579925.1"
                     /translation="MIRVALVDDQAIVRAGLARILSPADGFEVVAECADGWQAVEELP
                     DLHPDVVLMDIRMPALDGVSATAQLRAMEDPLCVLVLTTFGEDEVLWGAIEAGAAGFV
                     LKDSSAEDLIAAVRAVAGGGAWFDPGVAPRLLERYRRVVAPAARDSARLDLLTDREHE
                     VLRLMARGATNAEIAGALHVAEATVKTHVGSIFGKIGVRDRPAAIVFAYDHGVVTPGA
                     APP"
     gene            complement(3215..>3812)
                     /locus_tag="VF195_03540"
     CDS             complement(3215..>3812)
                     /locus_tag="VF195_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6579926.1"
                     /translation="DFVGHGLAAVMLQVTSARHVLRRDPGAAEDALRSAEEVGRRSMR
                     ELRRTVALLRSDDETGVEPPLPSVAEIPALVDQARAGGLAVELQTRGDLSSIPPGVGV
                     AVYRITQEALANAARHAPRAQTSLGLELTNGRVAIVAETTGPIVISSSSSERNRPRYG
                     LIGMRERATALGGEFAAGPTPNGWRMSCQLPVGDGRSR"
BASE COUNT          597 a         1346 c         1243 g          626 t
ORIGIN      
        1 agtccaggct ccaggtcgcg ttcgcttcgg ctccgagggg ctgccacagc ttcgtggcca
       61 tgtactcgga gaccgacatc ccggtggcgc gctcgagcac cagaccgagc agcagtggat
      121 ggtagttgtt gtagagccac cccccgccgg gtgggctcac gatctcggtc tcggcaaggc
      181 cgaggtcacg taggtcggtg ccgtagtacg tatcgacgtc gtccccccag ggcagaggga
      241 gcgactgctc ctcgtagcgg atgcccgagg tcatcgtgag caggtggcgc agggtgatct
      301 gctcgaaccg cggatcgcgt tcggagagtt cggggagata ttcggtcacc ggatcggtca
      361 cgtttccgat caacccctcc tcgatcgcga tcccgacgag cgtggaaagg aaggacttcg
      421 cgaccgagaa cgaggtctgg cgggtctggc ggtcggcgcc gaagtaacgc tcgtacacca
      481 gttggtcgtc gtccagaacc acgaagccga gcgtgccggt cccacgcagg aactcgtcga
      541 acgctcgatc atcctccgtg tccgaagtcg gcgcaggagg atcgatctcg tcgccggccg
      601 gaagcggact tgcctcatca ccggccgcga tggtcctggc cgggaaccga tactggtcgc
      661 cgacgtccgc ctcccgccac cacatcgccc gagcgatcga ggaccggtcg agcgatgccc
      721 acgcccatgc gtagacgccg accacgagca gggcgaccac gacgacgccc gccgccagcc
      781 gttgcagcca gcggcgccgc ggccgccccg ggccacgatc cgcttcgcgt ctggccctgc
      841 ctgtcgtcgc gcttgtctct cgcatatctc gcacatcctt cacggtgcgt aagagccctc
      901 ttccctttga cggccgcgca cgtgcaacct catcggtcgc caggtctttc gacctcagga
      961 cttggcatcg ggtcgtgtct cctggtcgcc ctgctcgccg aggtacctct ggacctggcg
     1021 ccgctcccag tcctttccga agaggccgcc gaggcctccc aggacgaccc ctgtcacgag
     1081 cacgccgagg cctgtgccga gcatcggcca gtagaaccag tagccgctcg agtcatccag
     1141 taggtcgatc gcgaaccaca tgacgctgag ggcgaggtag atgcccacga ccgtgtagaa
     1201 gatccgcttg cggcgaaccc accggcgagc ttcttcgcgc cggtcggtcg ggtcctcagc
     1261 catctcgcgc tgtggatctg ccacggtttc cccctttcat cgatggtgcg ccgcgccgga
     1321 agtctcggga agccacgatc aggccccggc gacggtcgcc gccatcttgt cgaggacctg
     1381 ctccatccca gctcgcgaga tctcgacgat ctccgggttc gggtagccgt attcgcggac
     1441 cgtcaatcgg gtagtggcgc tgtcgatcgg cgagagggtc acgacgtgcc gaacctcttc
     1501 ggggatcccc gggggcagtc cgagctcggc cggagcgacc cgggtgccgt cctcctttgc
     1561 gaagccctgg acgaactcga tccggtccat ggggtcgatg gaccgatacg tccacgtgtt
     1621 gtagagctcc cagtcctccg ctgaccgcat gcagacgacc gtcgtgcctc cctcacggaa
     1681 gtccatccgg cacatcggcg acgtgaagcc ctgcggcccc caccacctca tgacctgatc
     1741 ggcatcgctc cacgcccgcc acacgcgctc ttgcggcgca tcgaacgtgc gcgtcacgac
     1801 caggtcgtgc gtctcgttct cgatctgcat ccgtacctcc ttgcctttcc ctccgacgtc
     1861 gacaggcctc ggcgagtgga tcacgtcaag cgatgcccgc cgatgtcgta gggcacgttc
     1921 accgtttcgt cggcgccggg gttcgggcgc cagccgcccg agaaggtgcc ttcctcgcgc
     1981 caggagcccg tgaacgttgc gttcagctcg ccgtacgaca ccgcgatcgt cacggcatcg
     2041 cccaggacgt cccaccggta aggcaacggc tccggtgaga agccggagaa caccgtcgac
     2101 gggaagctgt cggtctcggg gtcgtaaccg atcaactcca aggcgtcgat ctgaaggccc
     2161 atgaagtcga tgtgcgcgcg ttgctcgagg aagaagccgc ccggcagcca gcggaacgtc
     2221 gtctcgccct tgatgttcct ttcatcggac ccgagcaggt tgccttccat ggtccagcgg
     2281 ccgacgaggc ggtccagtcg ccgaagagcg ggatcgggcg tcggtagctg tgcttgcgtg
     2341 tcgtctgcca tgagggtcct ccttccgtcc gtgacctgtg gggtgctcct gcggggcctt
     2401 catccgtagg tcgaagctgc ccggcggttc tcgacaaccg gccgacgtat cgcgccctgc
     2461 ttctccacgc agccgacgct agccctcgtc cggcagtctg acctcggtcc acgagcccga
     2521 gttccgctcc gacccaaggt ggagagcgtg cgctagggag gggctgcgcc aggggtgacc
     2581 acgccgtggt cataggcgaa gacgatggcc gcggggcggt cgcgcacccc gatcttgccg
     2641 aagatgctgc cgacatgggt cttcaccgtc gcctctgcaa cgtgcaacgc gccggcgatc
     2701 tcggcgttgg tcgcaccgcg ggccatgagg cgcaggacct cgtgctcacg gtcggtgagc
     2761 aggtcgagtc gagccgaatc acgggccgcc ggcgccacga cccgccgata gcgctcgagc
     2821 agccgcggcg cgacccccgg atcgaaccag gctccgcccc cggcgacggc ccgaaccgcg
     2881 gcgatcaggt cctccgcgga gctgtccttg agcacgaatc cggcggcccc tgcctcgatc
     2941 gccccccaca gcacctcgtc ctcgccgaac gtggtgagca ccaggacaca aagaggatcc
     3001 tccatggcac gcagttgggc cgttgcagat accccgtcga gagccggcat gcggatgtcc
     3061 atcaggacga cgtccgggtg caggtccggg agttcctcca ccgcctgcca gccgtcggcg
     3121 cactcggcga cgacttcgaa gccatccgca ggcgagagaa tcctggcgag gcccgcgcgg
     3181 acgatggcct ggtcgtcgac gagagcgacc cggatcatcg gctcctcccg tctcccaccg
     3241 gaagctgaca gctcatccgc cagccgttgg gtgtcggccc ggcggcgaac tcgccgccga
     3301 gcgccgtcgc ccgctcccgc ataccgatca ggccgtacct gggccggttg cgctcggagg
     3361 aggaggacga tatgacgatc ggccccgtgg tctccgcgac gatggctacc cgcccgttcg
     3421 tcagctcgag cccgagtgag gtctgcgcac gcggcgcgtg gcgagcggca ttcgccagtg
     3481 cctcttgggt gatccggtag acggcgaccc cgacgccggg tgggatcgac gacaggtctc
     3541 ctcgtgtctg cagctcgacg gcgagacctc cagcgcgtgc ctgatcgacg agcgcgggga
     3601 tctcggccac ggacgggaga ggtggctcga cgccggtctc gtcgtcgctc cggagcagcg
     3661 ccaccgtacg gcggagctcc cgcatgctgc gacggccgac ctcctcggcc gaccgcagcg
     3721 catcttcggc ggccccgggg tcgcggcgca ggacgtgccg agcactcgtg acctgcagca
     3781 tgacggctgc caacccgtgg cccacgaagt cg
//
LOCUS       DASUYD010000064         3737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_31610, whole genome shotgun sequence.
ACCESSION   DASUYD010000064 DASUYD010000000
VERSION     DASUYD010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31610"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF195_03545"
     CDS             complement(<1..357)
                     /locus_tag="VF195_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HEX6579927.1"
                     /translation="MERRIFGLENEYGVTCTFRGQRRLSPDEVARYLFRRVVHWGRSS
                     NVFLENGARLYLDVGSHPEYATPECDDVEELVAHDKAGERILEALLGAAELRLHEEGI
                     SGQVYLFKNNTDSAGNS"
     gene            complement(435..1121)
                     /gene="prcA"
                     /locus_tag="VF195_03550"
     CDS             complement(435..1121)
                     /gene="prcA"
                     /locus_tag="VF195_03550"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HEX6579928.1"
                     /translation="MSFMPYVSPDQLMKDRSEYAHKGIARGRSVIGLEFADGLLFIAE
                     NPSATLHKISEIYDRIAFAGVGKYSEFEDLRIAGIRLADLRGYSYGREDVKARDLANA
                     YSQALSTIFTQQMKPYEVEILVGEVNGEVDGTSIYHVLFDGSVTDEHGFVAIGGHADD
                     LTEELKERYNAGWDLATAVKIAVQVLGAPDQRTIEHDHIESGVLERSQSRRRKFRRLT
                     DDEIAGFLAG"
     gene            complement(1122..1916)
                     /gene="prcB"
                     /locus_tag="VF195_03555"
     CDS             complement(1122..1916)
                     /gene="prcB"
                     /locus_tag="VF195_03555"
                     /EC_number="3.4.25.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010850212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005839 - proteasome core complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit beta"
                     /protein_id="HEX6579929.1"
                     /translation="MTDRVELPLSGASFGADPSFVELLRRQHPEALPSSLQMSGALEV
                     AQGTTVLGLRFADGIVMAGDRRATAGYTIADAKMKKVFAADDFSAIAIAGAAGQAVET
                     VRLFQLELEHYEKLTGDRLSLEGKANRLAQMIRANFPLAMQGLVVVPLFGGYDVARSE
                     GRLFYYDATGGRWEEEDYQATGSGAQPAKNSLKKRWRSGLTHDDALRVATEALIDAAE
                     DDVATGGPDLARGILPVVVTVTALGATESTDEDVASAVRAVLEARS"
     gene            complement(1913..2113)
                     /locus_tag="VF195_03560"
     CDS             complement(1913..2113)
                     /locus_tag="VF195_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6579930.1"
                     /translation="MPEQERKQVKKGGAGGESDAEAGQVSATSKAAELKEEMDDILDE
                     IDSVLEENAEEFVKSYVQKGGE"
     gene            complement(2175..3659)
                     /gene="dop"
                     /locus_tag="VF195_03565"
     CDS             complement(2175..3659)
                     /gene="dop"
                     /locus_tag="VF195_03565"
                     /EC_number="3.5.1.119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="depupylase/deamidase Dop"
                     /protein_id="HEX6579931.1"
                     /translation="MAIAKVLGTETEYGIAAVGVPDFNPVLASSLLISTFAGSLRRIR
                     WDYEQESPLRDARGFEPIPSREVSDEDLGLANVILPNGARYYVDHAHPEYSTPECLTS
                     RDLVIHDKAGERILERSLREVATQLPDSPALLIYKNNSDGKGNSYGTHENYLVDRQTA
                     FADIVRDLTPFFVSRQVFCGAGKLGSEAPWDERGRPIYQLTQRADFFETEVGLETTLK
                     RPIINTRDEPHADPEKYRRLHVIVGDANMIEVAQFLKVGTTAIVLKMIEDRFLPDLSL
                     EGPVRALHEISRDLTCTVEVPLADGRRLNAVQLQWEYFEHAKKYVEREDDTPDQREVL
                     ERWEAILTSLESEPLSLHRELDWVAKFRLLEAYRERGGLDRGDPKLHAIDLQYHDVRR
                     EKGLYYRLERSDKVDRLVDEADVERAVMEPPEDTRAYFRGRCIGKYPDAIAAASWDSL
                     IIDTGAEALQRVPMREPLRGTKAHVDALLEASDDAASLVAALQG"
BASE COUNT          563 a         1287 c         1218 g          669 t
ORIGIN      
        1 ggagttgccc gccgagtcgg tgttgttctt gaagaggtag acctgaccgg agatgccttc
       61 ctcgtggagc cggagctcgg cggcgccgag gagcgcctcg aggatccgct cccccgcctt
      121 gtcgtgcgcc acgagctcct cgacgtcgtc gcattcgggc gtcgcgtact ccggatgcga
      181 cccgacatcg aggtacaggc gcgccccgtt ctcgaggaac acgttcgacg agcggcccca
      241 gtgcaccacg cggcggaaga ggtagcgcgc cacctcatcc ggagagagcc ggcgctgccc
      301 gcggaacgtg caggtcacgc cgtactcgtt ctcgaggccg aagatcctgc gctccatgcg
      361 acgagtctag gccagcccgc ggtctccgaa ggggcggctc catcgccacg gcgaggcctg
      421 aagaggcctc ctgctcaacc ggcgaggaag ccggcgatct cgtcgtcggt gagcctgcgg
      481 aacttgcggc gtcggctctg actgcgctcg agcacgcccg attcgatgtg gtcgtgctcg
      541 atcgtgcgct ggtcgggagc acccaacacc tgcacggcga tcttcaccgc cgtggccagg
      601 tcccatccgg cgttgtaccg ctccttcagc tcctcggtga ggtcgtccgc gtgacccccg
      661 atcgcgacga acccgtgctc gtcggtcacg ctcccgtcga acaggacatg atagatcgat
      721 gtgccgtcga cctcgccgtt cacctcgccc acgaggatct ccacctcgta cggtttcatc
      781 tgttgcgtga agatcgtcga aagggcctgc gagtaggcgt tcgcgagatc ccgcgccttg
      841 acgtcctccc gaccgtagga gtacccgcgg aggtccgcca gtcggatgcc ggcgatccgc
      901 aggtcctcga actcgctgta cttcccgacc cccgcgaacg cgatccgatc gtagatctcg
      961 ctgatcttgt gcagggtggc gctcgggttc tcggcgatga acaagagacc gtcggcgaac
     1021 tcgaggccga tcacggagcg cccgcgcgcg atccccttgt gggcgtactc cgaccgatcc
     1081 ttcatcagtt ggtcgggcga gacgtagggc atgaacgaca tctaggacct cgcttccagc
     1141 acggctcgga cggcgctcgc gacgtcctcg tcggtggact cggtcgcgcc cagcgccgtc
     1201 accgtgacga ccaccgggag gatgcctctg gcgaggtcgg ggccgcccgt cgccacgtcg
     1261 tcctcggccg catcgatcag cgcttcggtc gccacgcgga gcgcgtcatc gtgggtcagc
     1321 ccggacctcc accgcttctt cagggagttc ttcgcgggct gcgccccgga gccggtcgcc
     1381 tgatagtcct cttcctccca gcgtccaccg gtggcgtcgt agtagaagag cctgccctcg
     1441 ctcctcgcga cgtcgtagcc gccgaagagc gggaccacca cgagcccctg catcgcgagc
     1501 gggaagttgg cacggatcat ctgcgcgagc cggttcgcct tgccctccag ggacaggcga
     1561 tctccggtga gcttctcgta atgttcgagc tcgagctgga acagccggac ggtctcgacg
     1621 gcctggccag cggcaccggc gatcgcgatc gcggagaagt cgtcggcggc gaacaccttc
     1681 ttcatcttcg cgtccgcgat cgtgtagccg gccgtggctc gacggtcacc ggccatgacg
     1741 atcccgtccg cgaatcgcaa tccgaggacg gtcgtgccct gggcgacctc cagggccccg
     1801 ctcatctgaa gcgacgacgg gagcgcctcg ggatgctgac gacggaggag ctcgacgaag
     1861 ctggggtcgg cgccgaacga ggctcccgac agcgggagct cgacccgatc ggtcactcgc
     1921 cgcccttctg cacgtaggac ttcacgaact cctcggcgtt ctcttcgagg accgaatcga
     1981 tctcgtcgag gatgtcgtcc atctcctcct tgagctcggc ggccttcgag gtcgcggaga
     2041 cctggccggc ttcggcgtcg ctctcgccac cggcaccgcc cttcttgacc tgcttgcgct
     2101 cttgttcagg catggttcct cctcggtggc gattgtagcc gccgctcctc gcgggatcct
     2161 tcgaactgcc cgatctaccc ctggagtgcc gccacgaggg aggcggcgtc atcggaagcc
     2221 tcgagcaatg cgtccacgtg tgccttcgtt ccgcgcaacg gctctcgcat cgggacgcgc
     2281 tggagcgcct ccgcgccggt gtcgatgatg agggagtccc acgacgcggc cgcgatcgca
     2341 tccgggtact tgccgatgca tcgccctcgg aagtaggccc gcgtatcctc cggaggctcc
     2401 atcacggcgc gttccacgtc ggcttcgtcc accaggcggt cgaccttgtc cgagcgttcg
     2461 agccggtagt agagaccctt ctcgcggcgg acgtcgtggt actggagatc gatcgcgtgc
     2521 agcttcgggt cgccccggtc caggccgcct cgttcccgat aggcctcgag gagccggaac
     2581 ttcgccaccc agtccagctc gcgatggagc gagagcggct cgctctcgag cgaggtcagg
     2641 atcgcttccc agcgctcgag gacctcgcgc tggtcgggcg tgtcgtcctc gcgctcgacg
     2701 tacttcttcg cgtgctcgaa gtactcccat tggagctgca ccgcgttcaa tcgcctgccg
     2761 tcagcgagcg ggacctcgac cgtgcacgtc agatcccgcg agatctcgtg caacgctcgg
     2821 acgggcccct cgagggagag gtccggcagg aagcggtcct cgatcatctt gagcacgatc
     2881 gccgtcgtgc cgaccttcag gaactgggcg acctcgatca tgttggcgtc gccgacgatc
     2941 acgtgcaggc ggcgatactt ctccgggtcc gcgtgcggtt cgtcgcgggt gttgatgatc
     3001 ggccgcttca gcgtggtctc caggcccacc tcggtctcga agaaatcggc gcgctgcgtg
     3061 agctgataga tcggtcggcc gcgctcgtcc cacggcgcct cggaccccag cttcccggcg
     3121 ccgcagaaca cctggcggga cacgaagaac ggcgtcaggt cccgaacgat gtcggcgaac
     3181 gcggtctgcc gatcgacgag gtagttctcg tgtgtcccgt aggagttgcc cttgccgtcc
     3241 gagttgttct tgtagatcag cagcgccggt gagtccggca gctgcgtggc gacttcgcgc
     3301 agcgatcgtt cgaggatgcg ctcccccgcc ttgtcgtgga tcaccaggtc gcgcgacgtg
     3361 aggcactccg gtgtggagta ctcaggatgc gcgtgatcga cgtagtacct ggcaccgttg
     3421 ggaaggatca cgttcgccag ccccaggtcc tcgtcggaga cctcgcgtga cgggatcggt
     3481 tcgaagcccc tcgcgtcgcg gagcggcgac tcctgttcgt agtcccaacg gatccgccgg
     3541 agcgagcccg cgaacgtcga gatcagcagg gacgatgcga gcacgggatt gaagtccggc
     3601 acaccgaccg cggcgatgcc gtactccgtc tcggtgccca ggaccttcgc gatggccacc
     3661 cggcgaccct acagatattg gccgggactc acgcgctcga cctggcgacc gtcaccctcg
     3721 tcaccgagca gggtgcg
//
LOCUS       DASUYD010000065         8610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_32231, whole genome shotgun sequence.
ACCESSION   DASUYD010000065 DASUYD010000000
VERSION     DASUYD010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8610
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32231"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="VF195_03570"
     CDS             complement(<1..728)
                     /locus_tag="VF195_03570"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020753852.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6579932.1"
                     /translation="MNAVGAPLRRQGPGGDGTILDRSVPGRRAWSLPPLDVPEAPVPE
                     EHARGRRPGLPEVGEVDLVRHYTRLAQMNYGLDTGMYPLGSCTMKYNPKVAETVAALP
                     GFQRLHPLQPDSTTQGVLELLWRLERALCEITGMDEATLQPPAGACGEMTGLLLMRAF
                     HEDRGNARRRVIIPDSAHGTNPASVRLAGFEAVPVRSDARGLVDAGALAKLVDDSVAG
                     LMLTNPNTLGLFEEEIEEIAATIHG"
     gene            complement(725..2047)
                     /gene="gcvPA"
                     /locus_tag="VF195_03575"
     CDS             complement(725..2047)
                     /gene="gcvPA"
                     /locus_tag="VF195_03575"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012500520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEX6579933.1"
                     /translation="MKFAPHTDAEIDEMLAAIGIDSLDSLFGQIPPSVRLEGSLGLPD
                     GVSELEIVEDLRALAARDRHLDELVCFAGAGAYDHFIPAVVWALAARSEFSTSYTPYQ
                     PELSQGVLQVLFEFQSMVCELTGMDVSNASLYDGATALVEAVHMARGGERTRVLVSDG
                     VDPRLVETLRTYGSGSGYEPEIFASEDGRGGAPAVGDDVAAVVVQHPNVLGILEPASE
                     LFSVAREGGARAIQVFDPLSLGMLAPPGELGADIAVAEGQSLGNHLSFGGPYLGLIAA
                     RMSDVRRMPGRIVGETVDVDGTPGYVLTLQAREQHIRREKANSNICTNQTLMAVAATI
                     YLGWLGPEGLAEVGRRCAAKAAYAADRLTEIEGVSLLHPSAPFFKEFALRVPIAADRV
                     VAALVERGFLAGVPLPRDGDDVLLVAVTERRSKEQIDAFAEAMGEALP"
     gene            complement(2076..2462)
                     /locus_tag="VF195_03580"
     CDS             complement(2076..2462)
                     /locus_tag="VF195_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579934.1"
                     /translation="MSARAVTPVAPTLESLPKSDTIRFAHASERQFAQLLDFYGIVWD
                     YEPTSFDIEWDREGNVVRRFTPDFFLPEFDLYIEITTLNQKLVTRKNRKVRQLRERYP
                     EIRCKVFYQRDYLSLVTKYGLEDRSG"
     gene            complement(2489..2878)
                     /locus_tag="VF195_03585"
                     /pseudo
     CDS             complement(2489..2878)
                     /locus_tag="VF195_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009884633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            3078..3563
                     /locus_tag="VF195_03590"
     CDS             3078..3563
                     /locus_tag="VF195_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6579935.1"
                     /translation="MTRSRTALTKIALVAALMTGLTALAPAPAGAGTLSPRARMYRAT
                     NTSRVLNDVRRVDMHWRISKLARKHSIKMASTPDGSGGFGKLFHTKDPASYYLKGTSW
                     STWGENVGVTGGSVADLHHAFMDSPVHRANIVNQRFRRVALGTYRDDDGLLWVTVFFY
                     G"
     gene            complement(3583..4071)
                     /locus_tag="VF195_03595"
     CDS             complement(3583..4071)
                     /locus_tag="VF195_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEX6579936.1"
                     /translation="MIEMQLEGVRVELPTNQPIVLLRERDGERYLPIWIGASEAAAIA
                     LSLQGVQTPRPMTHDLMKNILEDLSVAVQKIVVTELRDSTFYATIELQRNGDHYEVSS
                     RPSDAIALAVRLAVPIFASEDVLDEAAILIPGEEDEEVEKFREFLDQVTPEDFKPEGS
                     PD"
     gene            complement(4117..4695)
                     /gene="hpt"
                     /locus_tag="VF195_03600"
     CDS             complement(4117..4695)
                     /gene="hpt"
                     /locus_tag="VF195_03600"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX6579937.1"
                     /translation="MTELARVLYGREDIHRRVDELGRTITGDYVGREPVLISVLKGGT
                     VFLADLMRQIPLPLETHLMAISPYGGHEVSLGRVRILLDVEIDLTDRDVVLVEDIVDT
                     GLTLSYLLSILRSRKPASLEVCTLLDRSIRRIVPLTPRYVGFDCPDSFVVGYGLDFEQ
                     RYRNLADILEISDTAALKADPDALLPHLAVPA"
     gene            complement(4769..6040)
                     /locus_tag="VF195_03605"
     CDS             complement(4769..6040)
                     /locus_tag="VF195_03605"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEX6579938.1"
                     /translation="MKRRVAAGAAAFLPLVLLIAQASWLHAIGAPAAPPPTPVPPNGS
                     PSPFVSRLSTPVDPVPVPRIDAPAVVLMDLAAGQILYQRAAVTARPIASLTKLMTALL
                     VLEAEEGRLDRTVRVDPEAVFARGEYGVGSSLGLRPGERVSVRGLLAGLLLGSANDAA
                     EALAIEESGSVGAFVAAMNARARALGMAETAFASPHGLDDRGLSSAFDILTLLREVRR
                     HTAFRRLVASRVVVVRSDAAPPRRIQNRNVMLWLYPGASGVKTGSTAGAGYCLVATAQ
                     RGGRELAAVVLGGRDEVFSEAAALLNHGFAAYHVRTLVSEGAQLGSVRVRGGTVPVVA
                     GEELEALVREQDTGAVERIVTASRSVAYPPASGSEVGTLRVTSGGTLLGQVPLLVADL
                     PPPEEPGGSWWGRAVGAVSSALGSVIADLMD"
     gene            complement(6037..6762)
                     /locus_tag="VF195_03610"
     CDS             complement(6037..6762)
                     /locus_tag="VF195_03610"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6579939.1"
                     /translation="MAGTRNYQSIGEVLVSVKTEFPDITISKIRFLEAEGLIEPERTP
                     SGYRKFYRHDVERLKSILRLQRDEYLPLKVIKERLLHQDAGVDDAGAASDGEATGSDD
                     ASATDEIADAPTGLQMSLEEMSTATGIDRDRIKELEAFGIISTHGPEGAHYYDGDDFI
                     ILSIVKDFFRYGIEARHLTMYKHFADRESSFFDQIVAPTLRQKNPDARRTAAQTLANL
                     SSTSRKFKQALLRTNLRDNLRSA"
     gene            complement(6770..7282)
                     /locus_tag="VF195_03615"
     CDS             complement(6770..7282)
                     /locus_tag="VF195_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6579940.1"
                     /translation="MPFNIERRQQVGEEGKQTVFCTRCGHPNRDDARFCAECGAPLQD
                     DSTVTLTPVEPDDEENVEFPFPEDELEPGQALLQVKRGPNAGSTFLLDTDVCTIGRNT
                     ESTVFLDDVTVSRAHAAFERKGGEWFVRDAGSLNGTYVNGEQVDETKLATGDQVQIGR
                     FRLTFFQAGE"
     gene            complement(7443..7826)
                     /gene="gcvH"
                     /locus_tag="VF195_03620"
     CDS             complement(7443..7826)
                     /gene="gcvH"
                     /locus_tag="VF195_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX6579941.1"
                     /translation="MEYPEDLRYSREHEWARSESGRVRVGITDFAQDALGDVVYVDLP
                     EPGTQVTSGQAFGEVESTKSVSDVFAPVSGTIVERNAALEEHPELVNEHPYGDGWLVA
                     IDPADPADVDTLLDAVAYREHISSS"
     gene            complement(7894..8472)
                     /locus_tag="VF195_03625"
     CDS             complement(7894..8472)
                     /locus_tag="VF195_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6579942.1"
                     /translation="MPASSAVVTVPNILSFARIATIPLFCWLAANERTRVWGILLFAV
                     VVSTDWVDGYVARRTGQVTELGRILDPVADRLAIAAGLVTFAIAGIFPFWAALLILVR
                     DVVVVLGGAVLLWGRNIRMEVRGIGKIATFSLMAAIAWIAWGNAEGPLGDVLLVGGWL
                     AYVVGIVEYYLAAGLYAIDVRDALAERDMREE"
     gene            complement(8497..8571)
                     /locus_tag="VF195_03630"
     tRNA            complement(8497..8571)
                     /locus_tag="VF195_03630"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8534..8536),aa:Pro,seq:ggg)
BASE COUNT         1413 a         2963 c         2871 g         1363 t
ORIGIN      
        1 ccgtggatcg tcgccgcgat ctcctcgatc tcctcctcga acagaccgag ggtgttcggg
       61 ttggtgagca tcagaccggc cacggagtcg tccacgagct tcgccagcgc cccggcgtcg
      121 accagaccac gggcgtcgga gcggaccggc accgcctcga atcccgccag ccgaacgctg
      181 gcgggattcg tcccgtgagc ggaatccggg atgatcacgc gacgcctcgc gttgccgcga
      241 tcctcgtgga acgcacgcat caggagcaga ccggtcatct cgccgcacgc gccggccggc
      301 ggctgcagcg tcgcctcgtc catccccgtg atctcgcaca gagcccgctc caggcgccac
      361 aggagctcca gcacgccctg ggtggtgctg tccggctgga gtgggtggag ccgctggaaa
      421 cccgggaggg cggcgaccgt ctcggcgacc ttcgggttgt acttcatcgt gcacgagccg
      481 agggggtaca tcccggtatc gaggccgtag ttcatctgcg ccaggcgcgt gtagtggcgg
      541 acgaggtcca cctcccccac ttcgggcagg ccgggacgac gtccgcgggc gtgctcctcc
      601 ggcacgggag cctcgggaac gtccaggggg ggcaggctcc aggcccggcg gccggggacg
      661 gaccggtcga ggatcgtgcc gtcgcctccg gggccttgcc ggcggagcgg cgcacccacc
      721 gcgttcacgg aagcgcctcc cccatcgcct cggcgaaggc gtcgatctgc tccttgctcc
      781 gccgttcggt cacggccacg agcaggacgt catctccatc ccgtggcagg gggacgcccg
      841 cgaggaatcc gcgctccacg agagcggcga cgacgcggtc cgcagcgatg ggtacccgga
      901 gcgcgaactc cttgaagaac ggcgcggagg gatgcaggag tgagacgccc tcgatctcgg
      961 tgagccggtc cgcggcgtag gcggccttcg cggcgcatcg gcgcccgacc tcggcgaggc
     1021 cctccggacc gagccacccg aggtagatcg tggccgccac ggccatcagg gtctggttcg
     1081 tgcagatgtt cgagttcgcc ttctcacgac ggatgtgctg ctctcgcgcc tgcagtgtga
     1141 gcacgtatcc gggcgtgccg tcgacgtcga cggtctctcc cacgatcctg ccgggcatcc
     1201 ggcgcacgtc cgacatcctg gcggcgatga gcccgaggta cgggccgccg aaggagagat
     1261 ggttcccgag agactggccc tcggcgacgg cgatgtccgc gccgagctcc cccggcgggg
     1321 ccagcatccc cagggagagc gggtcgaaga cctggatggc cctggcgccg ccctctctcg
     1381 ccacggagaa gagctcggaa gccggctcga ggatccccag gacgttgggg tgctgaacga
     1441 cgacggccgc gacgtcgtct ccgacggccg gcgcccctcc ccgaccatcc tcggatgcga
     1501 agatctccgg ctcgtacccg gatccactcc cgtacgtccg gagggtctcg accagcctcg
     1561 ggtccacgcc gtccgagacg aggacccggg tccgctcgcc tcccctggcc atgtgcacgg
     1621 cctccacgag cgccgtcgcg ccgtcgtaga gcgaggcgtt cgacacgtcc atcccggtga
     1681 gctcgcacac catcgattgg aactcgaaga ggacctggag cacgccttgc gagagctccg
     1741 gctgatacgg cgtgtacgag gtcgagaact ccgatcgggc ggcgagcgcc cacaccacgg
     1801 cggggatgaa gtggtcgtat gcgcccgcgc cggcgaagca caccagctcg tccagatgcc
     1861 gatcgcgcgc ggcgagcgcg cggaggtcct cgacgatctc gagctccgag acgccgtccg
     1921 ggagcccgag cgacccctcg agacggaccg agggggggat ctgcccgaac agcgagtcga
     1981 gagaatcgat gccgatcgcc gcgagcatct cgtcgatctc ggcatcggta tgcggggcga
     2041 acttcacggc gagcagtgta cgggcaggcc gcggctcagc ctgagcggtc ctcgagcccg
     2101 tacttcgtga cgagcgagag gtagtcacgc tgatagaaga ccttgcaccg gatctccgga
     2161 tagcgctcgc gcagctgacg gaccttccgg ttcttcctgg tgacgagctt ctggttcagc
     2221 gtggtgatct cgatgtacag gtcgaactcc gggaggaaga agtcgggggt gaagcggcgc
     2281 acgacgttgc cttcacggtc ccattcgatg tcgaacgacg tcggttcgta gtcccagacg
     2341 atgccgtaga agtcgagcag ctgcgcgaac tggcgctcgg aagcgtgggc gaaacggatc
     2401 gtatcgctct tggggaggga ctccaacgtg ggtgcgaccg gggtcacggc tcgcgcgctc
     2461 acacccctcc tgcggccgcc cgtctgcgtt tcgagagggt tccctccagg tcgctccaga
     2521 cctggtcgag agcgatggcg aagacaccct gtcccttctg gaggaggtcg atcacggcct
     2581 ccgccgaatg ggcctcgtag accccacggc cgtccgacat caaggtgacg cggtgcgtag
     2641 gccgcttcat cgaatgcaga tgctcgacgg ccttgcggac ccgctggagg gacacccccg
     2701 tatccaggag cttcttgatg accttcagcg agacgaggtc ctggaacgag tacagccgct
     2761 gcgtccccga tcctcgcgcg tcgcggatcg agggcgtcac cagctccgtc ctggcccaat
     2821 agtcgagctg ccgatagctg atgccgacga tccgacagac gtccggtacc cggaacccct
     2881 cttcgctcgt catcgctccc cgctcatcac agctcggtaa ttacgacggt gtcgttgcag
     2941 cgtatgcccg agggaaatac gtgtcaatgg aaggggacga tcatccggta ggccgcggct
     3001 caagttctca gcgcccgtcg tcgatggaat agctgcacta cccggaacta ggcacccaga
     3061 gcacgaggga ctgacggttg acacgcagcc gaaccgcact cacgaagatc gccctggtgg
     3121 ctgctctcat gacgggcctg accgcgctcg ctcccgctcc ggcgggcgcc gggacactct
     3181 cgcccagggc gcggatgtac agggccacca acaccagccg cgtgctcaac gacgtccgac
     3241 gggtggacat gcactggcgg atctcgaagc tcgcgcggaa gcacagcatc aagatggcga
     3301 gtactccgga tggttccgga gggttcggca agctcttcca cacgaaggac cctgccagct
     3361 actacctcaa ggggaccagc tggtcgacct ggggagagaa cgtcggcgtc accggtgggt
     3421 cggtcgcgga tctgcatcat gcgttcatgg actcccccgt ccaccgcgcg aacatcgtga
     3481 accaacgttt ccggcgcgtc gcgctcggca cctaccggga cgacgacggc ctgctctggg
     3541 tgaccgtctt cttctacggc tgatcttcct cgatcggcga actcagtctg gcgagccttc
     3601 gggcttgaag tcctcggggg tgacctggtc gaggaactcc cggaacttct cgacctcctc
     3661 gtcttcctcg ccggggatca ggatcgccgc ctcgtcgagc acgtcttcgc tggcgaagat
     3721 cgggacggcg agccgcacgg ccaaggcgat cgcatctgag ggtcgcgagc tcacctcgta
     3781 atgatccccg ttccgctgga gctcgatcgt cgcgtagaac gtcgagtcac ggagctcggt
     3841 cacgacgatc ttctgcacgg cgacggagag atcctcgagg atgttcttca tcaggtcgtg
     3901 ggtcatcggc ctgggggtct ggaccccctg cagtgagaga gcgatcgcgg ccgcctcgga
     3961 ggccccgatc cagatcggca ggtaccgctc cccgtctcgc tcccggagca acacgatcgg
     4021 ctggttggtc ggaagctcca cgcgaacgcc ttccagctgc atctcgatca tgtgctggca
     4081 ctgtacgagg ggcctgcgac cgagcgcaac ctcctctcag gccggaacgg cgagatgcgg
     4141 gagcagagcg tcgggatcgg ccttgagcgc cgccgtatcg gagatctcca ggatgtcggc
     4201 caggttccgg tagcgctgct cgaagtcgag gccgtatccg acgacgaagc tgtcggggca
     4261 gtcgaacccc acgtaccggg gggtgagcgg gacgatccgt cggatcgatc gatcgaggag
     4321 cgtgcacacc tccagcgaag cgggcttgcg ggaccgcagg atcgacaaga gataggagag
     4381 cgtgagcccg gtgtcgacga tgtcttcgac cagcacgacg tcgcggtccg tcagatcgat
     4441 ctcgacgtcg aggaggatgc gaacccgtcc cagcgacacc tcatggccgc cgtaggggct
     4501 gatcgccatc agatgggtct cgagcggcag cgggatctgg cgcatcagat ccgcgaggaa
     4561 caccgtaccg cctttgagca ccgagatgag gacgggctca cgccccacgt agtccccggt
     4621 gatcgtgcgg ccgagctcgt ccacgcggcg gtggatgtcc tcgcgcccgt acaacacgcg
     4681 agcgagctcc gtcatcgggt tcggagccta gcatccgttc ggccggagct gtagagcccg
     4741 gccagggtcg gcggcgaacc cgcgtcgctc aatccatgag atcggcgatc acgctgccca
     4801 gcgcggacga gacggctccg acggcccgac cccaccagga gccgcctggc tcctccgggg
     4861 gagggaggtc cgccaccagg aggggcacct gcccgagaag ggttccgccc gaggtcacgc
     4921 ggagcgtccc cacttcggag ccgctcgcgg gagggtacgc gaccgacctc gatgccgtga
     4981 cgatccgctc gaccgccccg gtgtcctgct cgcgcacgag ggcctccagc tcctcccccg
     5041 ccacgacggg cacggtgcca ccacgcacgc gaacgctccc gagctgcgct ccttccgaga
     5101 ccaaggtccg cacgtggtac gcggcgaaac cgtggttcag gagcgcggcc gcctcggaga
     5161 acacctcgtc gcgaccgccg aggacgacgg cagcgagctc tcgcccgccg cgttgggcgg
     5221 tcgcgacgag gcagtacccg gcgccggccg tggacccggt cttcactccg gaagcccccg
     5281 gatagagcca cagcatcacg tttcgattct ggatccgccg cggcggcgcc gcgtccgagc
     5341 ggacgacgac gaccctgctg gccacgagcc gccggaaggc cgtgtgcctg cgaacctctc
     5401 gcagcaacgt gaggatgtcg aatgccgacg acagacctcg gtcgtccaac ccgtggggag
     5461 acgcgaaggc cgtctccgcc atcccgagag cgcgcgctcg cgcattcatc gccgcgacga
     5521 acgcgccgac gctccccgac tcctcgatgg cgagcgcctc tgcggcgtcg ttcgcggacc
     5581 cgaggagcag accggcgagc aatccccgga cagagacccg ctcacctggc cggaggccga
     5641 gcgacgaccc caccccgtac tccccccggg cgaagacggc ctcaggatcg acacggacgg
     5701 ttcggtccag acgcccctcc tcggcttcga ggacgagcaa cgccgtcatc agcttcgtca
     5761 ggctcgcgat cggtcgcgcc gtcaccgccg ctcgctgata caggatctgc ccggctgcga
     5821 gatccatcag gacgacggcc ggagcatcga tccgtgggac cgggaccgga tcgacgggcg
     5881 tggagagccg cgacacgaac ggggacggcg atccgttggg cgggaccggc gtcgggggcg
     5941 gcgcggccgg ggcgccgatc gcgtgcagcc acgacgcctg ggcgatcagg agcacgagtg
     6001 gcaggaaggc ggccgcgccg gcggcgaccc tccgcttcac gcgctgcgaa ggttgtcgcg
     6061 caggttcgtc cgcagaaggg cttgcttgaa cttccgcgac gtggacgaca ggttcgccag
     6121 ggtttgggcc gcggtgcggc gagcgtccgg gttcttctgc cggagggtcg gcgccacgat
     6181 ctggtcgaag aaggacgact cccggtccgc gaagtgcttg tacatcgtga gatgacgagc
     6241 ctcgatcccg tagcggaaga agtccttcac gatcgacagg atgatgaagt cgtccccgtc
     6301 gtaataatgg gcaccctccg ggccgtgcgt ggagatgatc ccgaacgctt cgagctcctt
     6361 gatccggtcg cgatcgatcc cggtggcggt ggacatctcc tcgagcgaca tctgcagccc
     6421 ggtcggcgcg tccgcgatct cgtcggtcgc cgacgcgtcg tcgcttccgg tggcttctcc
     6481 gtcggatgcg gcccccgcgt cgtcgacccc agcgtcctga tggagcagcc gttccttgat
     6541 caccttcaga ggaaggtact cgtcgcgctg gagccggagg atcgacttca gccgctcgac
     6601 gtcgtgccgg tagaacttgc ggtagccgga cggcgtccgc tcgggctcga tcaagccctc
     6661 ggcctcgagg aaccggatct tcgagatcgt gatgtccggg aactccgtct tcacggacac
     6721 caggacctca ccgatcgact ggtagttgcg cgtgccggcc atctttacct cattcccctg
     6781 cctggaagaa cgtcagcctg aagcgtccga tctggacctg gtcgccggtg gccaacttcg
     6841 tctcgtcgac ctgttcgccg ttcacgtacg tcccgttgag gctccccgca tcgcggacga
     6901 accactcccc acctttacgc tcgaacgcgg cgtgcgcacg ggagacggtg acgtcgtcca
     6961 ggaacaccgt gctctccgtg ttccgcccga tcgtgcacac gtccgtgtcg agcaggaacg
     7021 tcgatcctgc gttggggcca cgcttgacct gaaggagcgc ctgcccgggt tcgagctcgt
     7081 cttcggggaa ggggaactcg acgttctctt cgtcgtcggg ttcgaccggg gtgagcgtga
     7141 ccgtcgagtc gtcctgaagg ggagctccgc actccgcgca gaagcgtgcg tcgtcccggt
     7201 tcggatgtcc acaacgagta cagaacaccg tctgcttccc ctcttccccg acctgctgtc
     7261 gacgttcgat gttgaagggc aaccgtcgac ctcgacgcgt cggggacgtt atcgcagcag
     7321 gtaaggaggg tcaaccgagc cttcgccgta ccctcgacct cgctcccccg tcaccctcga
     7381 cctgggttcc ctgtgaccct tgacctgcgc tggagtatcg gtcgagcgtc gagggtccct
     7441 gctcaggagc tcgagatgtg ctcccggtat gccacggcgt cgaggagcgt gtcgacatcg
     7501 gccggatccg ccggatcgat cgcgaccagc cagccgtccc cgtagggatg ctcgttcacg
     7561 agctccgggt gctcttccag ggccgcgttg cgttccacga tcgttccgga gaccggcgcg
     7621 aacacgtcgc tgacggattt cgtcgactcc acctccccga acgcctgacc gctcgtcacc
     7681 tgcgtgccgg gctcgggaag gtcgacgtac acgacgtcgc ccagcgcgtc ctgggcgaag
     7741 tcggtgatgc ccacgcggac gcgtccgctc tccgagcgcg cccactcgtg ctctcgcgaa
     7801 tacctcagat cttcggggta ctccactcgt cctccgttct cggttcgccg tgacactcgg
     7861 gtcggtgacc actgccgcgg gctgggcgtg aacttactcc tctcgcatgt cgcgctccgc
     7921 gagggcgtcg cggacgtcga tcgcgtagag gccggcggcg aggtagtact ccacgatccc
     7981 caccacgtac gcgagccagc ctccgacgag gaggacatcg ccgagcgggc cctccgcgtt
     8041 gccccaggcg atccacgcga tggcggccat caaggagaac gtggcgatct tgccgatccc
     8101 gcggacctcc atccggatgt tcctccccca gagcagcacg gcgccgccga gcacgacgac
     8161 gacgtcgcga accaggatca acagcgccgc ccagaacggg aagatccccg cgatcgcgaa
     8221 cgtcacgagg ccggccgcga tcgcgagccg gtcggcgacc ggatcgagga tgcgtccgag
     8281 ctcggtgacc tggcccgtcc gacgcgcgac gtacccgtcg acccaatcgg tgctgaccac
     8341 cacggcgaac agcaggatcc cccagaccct cgtccgctcg ttcgccgcca gccagcagaa
     8401 cagagggatc gtggcgatgc gggcgaacga caggatgttg gggacggtga cgacggcgga
     8461 ggatgccggc atcgtctccg tcgtccctct catccgtcgg gccggccgga ttcgaaccgg
     8521 cgaccttctg acccccagtc agacgcgcta accaagctgc gccacggccc gtacgtggca
     8581 gtgtagccgt gcggggttgc ggctccacgc
//
LOCUS       DASUYD010000066        21113 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_254236, whole genome shotgun sequence.
ACCESSION   DASUYD010000066 DASUYD010000000
VERSION     DASUYD010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21113
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254236"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1225
                     /locus_tag="VF195_03635"
     CDS             38..1225
                     /locus_tag="VF195_03635"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX6579943.1"
                     /translation="MALRTLDDLGDVAGSRVFVRVDFNVPLDGGRVADDLRIRAAIPT
                     LRELRARGAALVVASHLGRPEGKARDDLRLAPVADRLGELLEVEVMALDEVVGDDPEA
                     VCAHIDQGDVVLLENLRFDPGEESNDVRFAGRLAELADAYVDDAFGAAHRAHASVVAL
                     PDLMRASGRTTAAGRLLQREVEVLGRLLRGPDRPYVAVLGGAKVSDKLGVLGALAERA
                     DALLIGGAMAFTLIAADGGEIGDSPVERDRFDEVRAVMHAGDALIELPQDVVAATEAS
                     ADVRPQTVPAREIPRGLKGLDIGPRTVEEFARILADAKTILWNGPMGVFELEPFSAGT
                     RGVAVAIAGAAAFSVVGGGDSLLAVKREGLEDSFDHLSTGGGASLDFLEGTPLPGITV
                     LQD"
     gene            1241..2011
                     /gene="tpiA"
                     /locus_tag="VF195_03640"
     CDS             1241..2011
                     /gene="tpiA"
                     /locus_tag="VF195_03640"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017535440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX6579944.1"
                     /translation="MADRTPMIAANWKMHKTHLESIQAVQKLSYLLDRKDAERVEVVI
                     CPAFTALRSVQTLIDSDRLPYGLGAQDVHPEDDGAFTGAVAGPMLAALDVAYVIVGHS
                     ERRQLFGEDDATVNRKVKAVIRNGMTPIVCVGETLEERESGGTQTKVADQVRRAFDGI
                     DPTAAATFVVAYEPIWAIGTGRNAEPTDAGQVVEVIRATLADRVSSEAAGAVRVLYGG
                     SVKPGNIREFMAHPEIDGALVGGASLDPESLALIARYR"
     gene            2115..3161
                     /locus_tag="VF195_03645"
     CDS             2115..3161
                     /locus_tag="VF195_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013153612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6579945.1"
                     /translation="MSRSDTDTDVSQAEQREAAERADLEAAAPSAETPASIIGRSPWE
                     IFWSHFRQDKFALMGLVIIGVMILMAIFAPLVASWSGHEPNQVNLEALDEFGLPSAPT
                     WWPGPEAKAPAKYLLGVDDTGRDLFVRIAYGARTSLTVAFFATGIAVVLGVLMGLTAG
                     FYRGKIDTIISRATDVVLALPILLLALGIASACGANQEGCFWGFIKPGLRPVILIIGL
                     FGWPYIGRIVRGQVLSIREKEFVEASRSLGASNRRIILREILPNVVAPIIVYTTLIIP
                     SNILFEAGLSFLGVGVPDGTPSWGAMLADAGTAFRWAPWLMIFPGLALFLTTLAFNLV
                     GDGLRDALDPRKAV"
     gene            3258..5129
                     /locus_tag="VF195_03650"
     CDS             3258..5129
                     /locus_tag="VF195_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6579946.1"
                     /translation="MKRVFRLIAVVTVLGIVAAACGGDGTGDTPTDEPSQPADLSGGT
                     LTMAMLADVTAAFDPQKEYYSVTWEYYRCCLLRNLMSYEGVPTDEGGADIFPDLAVDE
                     PTQSSDGLTWTYQIKPGINYAPPKEDVEITAQDFIRALERTANPDANVGGYSFYYSVI
                     EGFDDFAAGDAEEISGLAAPDTHTLEITTTSPVGDMSYRLAMGTASPIPPDDEGERMG
                     VAEGHDRNYGRFLVASGPYMFEGSETMDFSADPKDQTEAPGYVPGRQIVLVRNPSYDP
                     ATDGLRDAYPDRIEVRIGGDNDDLYNQVAAGTLDFVVDGVVPAEKIREYQTDPELQDK
                     INIYASDAIRYVSFNLAVPPFDDVHVRKAVSWAFDKQGFRQLRGGPSVGEIAGHIMVN
                     SLENNLLADYDPYQTTNASGDPEKAKEEMAQSKYDTDGDGVCDDPSCQDILTITDRED
                     PYPDQMALLKQFLEPLGMTLDDKQLERGTMYNQCNDANSHHALCAGPGWGKDYADGVT
                     FGEPLFTSAGLWESCCNYSLLGASPEQLTEWGYDVTEVPSIDDKEDECAALEPGDERF
                     QCWAELDQQLMEEIVPMVPYLFDNSVDILSENIVNYSFDQFAGLAAFDSMAVPGNTG"
     gene            5223..6257
                     /locus_tag="VF195_03655"
     CDS             5223..6257
                     /locus_tag="VF195_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6579947.1"
                     /translation="MGRYLIRRALFMILVIIIVSLLTFLIFVKLPAGDPARRAVGRTT
                     TPQQIENARRAFGLDKPLYIQYWRFLRGFIPWPPGDPVLMLSGHPPRSHEGLWLNEDV
                     YYSYDGFVPVREELARRIPITGTLAIGAAVIWLLLGIPIGILSGVRRRSVADRAAMIF
                     AIVGVSMPIFWLAQLLLYAFWFKLELLPESGIPIGYSGWGGAMRAALEGRFILPWVSL
                     AYINAAFYARMVRGNLIETMNEDYIRTARAKGLSERRVIYKHGLRGALTPVVTMLGLD
                     VGLLLGGAFITETVFGLPGIGQKAVQAIGTNDFPTVMGVTILGAFIIAVANLVVDVAY
                     AFVDPRVRYT"
     gene            6258..7271
                     /locus_tag="VF195_03660"
     CDS             6258..7271
                     /locus_tag="VF195_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6579948.1"
                     /translation="MAQPLLEVKDLKVHFPTDDGLVKAVDGVSYSLAAGETLGIVGES
                     GSGKSVSSLTVMGLINPKQATISGEVFFQGQDLLKLPKDEMRTIRGAKISMIFQDPMT
                     SLHPFYKVGYQIAEAILEHQDISKKEAHDQAVEMLARVNIPHPKDRADQYPHEFSGGM
                     RQRAMIAMALSLNPDVLIADEPTTALDVTVQAQILDLIDRLKSEFNAAVVIITHDLGV
                     VAEHCDNIMVMYAGKAAEFGDTDDIYYKPLHPYTWGLLSSIPRLDEEQQERLNPIRGL
                     PPSLIQVPPGCAFHPRCPYRFDPCDRDVPELLPVDGHHAAACHLSLADKERIRDQEIL
                     AHR"
     gene            7268..8434
                     /locus_tag="VF195_03665"
     CDS             7268..8434
                     /locus_tag="VF195_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018553193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEX6579949.1"
                     /translation="MSVQAQPVPAQPPAPSGEPLVKIEGVKKYFPITQGILFQHHVGN
                     VHAVDGVDLEIYPGQTVGLVGETGCGKSTLARVVMRLYDATEGTIWFQGQDITHLKGK
                     ELQELREDMQMVFQDPYASLNPRKTVGTIVSEPFRLHQTVPKNRIKSEVQQLMELVGL
                     NPEHYNRYPHEFSGGQRQRIGVARSLALRPKLIVCDEPVSALDVSIQAQILNLLGDLQ
                     DEFNLTYLFIAHDLSVVKHVSDRVAVMYLGKIVEMAEGPALYKTPKHPYSGALLSAVP
                     MADPELARQKRRIILQGDVPSPIDPPSGCRFHPRCPQSRAIAKAEGIDGPHPRCVQEE
                     PELSDQGAGQVAACHFPLEGSIIEGGDAEARFEDGVVEMQEGLSGSEDTPQDGP"
     gene            8479..8709
                     /gene="secG"
                     /locus_tag="VF195_03670"
     CDS             8479..8709
                     /gene="secG"
                     /locus_tag="VF195_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009881371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX6579950.1"
                     /translation="MNAVVIVINIIASLFLILSILLHSGRGSGMSELFGGSSTLAGGT
                     ALERTLDRVTVVTAIVWGLCTFWLAWKWTPGS"
     gene            8736..8822
                     /locus_tag="VF195_03675"
     tRNA            8736..8822
                     /locus_tag="VF195_03675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8771..8773,aa:Leu,seq:gag)
     gene            complement(9127..10044)
                     /locus_tag="VF195_03680"
     CDS             complement(9127..10044)
                     /locus_tag="VF195_03680"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6579951.1"
                     /translation="MSTTLERPPKLQRPEGPAASPRLGFIKRHALSAYYVLVFTISLG
                     GILLVLGPDGLFSTGSTASAAIVLVIIGPPVASLLLTALVDGRAGLRELGSRLRRWRV
                     GARWYGVALLTAPLVMGATVFALSLASPEFRPDIVTADDKLGIVVIGIAAGLVGPFLE
                     ELGWTGFALPRLRRRYSVLTTGLVMGVLWGAWHFPLFAGDTDPEGVVPSALLVVALLF
                     AWLIPYRVLMVWVYDRTQSVLLMYLMHVPATAFVAISVPSEGVSGIVLVTPVLIWGAV
                     FSMMVAAVAWANGGHLTGRQHAAPTMRSA"
     gene            complement(10567..10899)
                     /locus_tag="VF195_03685"
     CDS             complement(10567..10899)
                     /locus_tag="VF195_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011029816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6579952.1"
                     /translation="MQKLSLDSLARQELKRAANTESGRSAETIVGGHAKVLRQTVIAM
                     MKGVVHNEHESPGEATVQVLHGRVRISAGDSSWEGRTGTVLIVPDARHKLEALEDAAI
                     LLTVAKPG"
     gene            complement(11499..13802)
                     /locus_tag="VF195_03690"
     CDS             complement(11499..13802)
                     /locus_tag="VF195_03690"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6579953.1"
                     /translation="MANASLKRVLVGRPLASHKAEHQLLPKTLALPVFSSDALSSNAY
                     ATEEMMLVLVAAGAGALAFRVPIAIAIAIVLVIVVTSYRQTVRAYPGGGGSYIVAHDN
                     LGTVPGLVAAAALLTDYVLTVAVSIVAGFLAITSAVPSLAGHKVILSIGLVVLVTLAN
                     LRGVKEAGVLFAIPTYGFVAIVYVLLGVGFLRCLGGCPQAATAHLPLEATAPLSLFLV
                     LKAFSSGATALTGVEAISNGVPAFRRPQAKNAARTLSMMAVMTVSMFLGITVLTTLLD
                     VRVSHEIAASRSVLSQIGETVFGRTFLYFVLQGLTAGILILAANTAYQDFPRLSAILA
                     RDRFMPSQFRNRGDRLVFSNGIIILALAASGLVYVFDADLTKLIQLYVVGVFTAFTLS
                     QLGMVRRWLRLRAPGWRRSMIINAIGATATGVVLVIVTITKFSGGAWIVLAAMPVIIA
                     FFLAVHRHYGSIGRALRLRTRPPIRGAPNTFVMLVRDLGPATAKAVGYVRALRPEHVT
                     PLFVGDPEAFESAAERWPALAPRLGALQLLDAADGRPMPALRARLRSLRKDAEGFVTV
                     VVAEEIRSRSLLRHLGRRWGFWLKVTLFFEAGIVVADVTFLPEEAARDATPPLEPSRH
                     VTLVPVSAVHAASVEAVSYALSLEATEVAALFFSTDRGDERELAAAWIDHRMSIPLAI
                     VDAPFRDLAEPMLEEVRRYTSREGTIVSVVLPEVVVSHWWEQVLHSQSALFFKRLLLF
                     EPGVVVVSVPLHVTQQETEPMAVESGR"
     gene            complement(13784..14074)
                     /locus_tag="VF195_03695"
     CDS             complement(13784..14074)
                     /locus_tag="VF195_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579954.1"
                     /translation="MNWLSILAAGGGGDLLVGILIGCCLGILIGPGFRAWQTHREWVD
                     ASREAGLTDRLLDRIERDAGPDVWRALSELDDAELDDAELDDATEPTWRTRR"
     gene            14248..15618
                     /locus_tag="VF195_03700"
     CDS             14248..15618
                     /locus_tag="VF195_03700"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579955.1"
                     /translation="MTALAPPGLVTLGAVLPVDIPTTTAALCYVLAVVVATATGGLVA
                     GLVSSVASFLALNFFFTPPFHTFAVEETADLVALAVFLAVSVTVGTMFSRALEQRARA
                     ERREREARLLNHLGTRLRSGVPTEDVLRSLARSILELFDLARCEISSELSGRPIVAEN
                     PDRTSTGGREEVMPMTVQDRELGRILVVVGDGRPAMTAEERGVLQTLASQMALAMDGM
                     RLGSEAEEARMEAETNRLRAALFSSVTHDLRTPLASITASVSTLLEDRSPLHDDQRRE
                     LLETIDQETGRLNRVIGNLMDLSRMRAGAVMPTKSPAAIEEVIESVLARSGPILERHD
                     LRLMIREDLPEIPLDVVLIDQALTNIVENAARFTPPGRRITIGAARWRDGVQVRIADR
                     GPGIPREERDRVFEPFVRGEGSTGTGLGLAIARAIVEAHGGSIRIGEEPGGGTAVVLE
                     LPGGST"
     gene            15615..16328
                     /locus_tag="VF195_03705"
     CDS             15615..16328
                     /locus_tag="VF195_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6579956.1"
                     /translation="MKEGRVLVVDDDPQILRAVRTSLLGHDYEVLTAGNGETALHLLP
                     DADVDLVVLDLGLPGIGGDEVIRRLRSWSDVPIIVLSVREGQHDKVGAFEAGADDYVT
                     KPFAMPELLARMRAVRRRVEGERRPSVIRFDDLEVDLGRQLVRIGGTDVHLTPTEYRL
                     LEAFVTNPGKLLTHRWLLQRAWGSGYATEHQYLRVFIRQLRAKLSDDPARPRFIVTEA
                     GLGYRWKPDPDLDPPPAVS"
     gene            16333..16689
                     /locus_tag="VF195_03710"
     CDS             16333..16689
                     /locus_tag="VF195_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579957.1"
                     /translation="MPIIEIHGLPPDRGLDVPTALADVTSAVAAYLGEEPRGTWAIWR
                     PVEPGAYAEGADAPSTQPRGTHPAIVDVFAGRRDDTPGLLHAVGRAVVEAFGLDEGNV
                     VVRLTEAEPGRVYWGD"
     gene            16853..18448
                     /locus_tag="VF195_03715"
     CDS             16853..18448
                     /locus_tag="VF195_03715"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX6579958.1"
                     /translation="MNALDVRGLSVDAGGLTVVRDLDLAISSGDKVGVVGRNGAGKTS
                     TLKVLAGEMPPSEGTVERCGALGYLRQDPRQDRSDDSACGIEYLLAARGLVELQRGVE
                     KARLQLEEHASEDRVARFARLEEEYDRLGGYRAESDARTIVAGLGLSQDRLGLPVGTL
                     SGGERRRLELARILFGGSDLLLLDEPTNHLDADAKGWLMRFLAGYRGALLVVSHDIAL
                     LDASITRILHLDRDGVVEYRGTYTQYREARARDEKRLTALASRQETEIKRLKTLADSM
                     RGQTAKRARKAKTLDTRVARLQARKVAAPTRERGVRFRFPEPPHSGRTMLSVDGLTKG
                     YGGPAVFEDVSFEVERGERLLVMGLNGAGKTSLLRILAGETRADAGTFRLGHGAQLGY
                     YAQEHEGIRERMTVLSHMHQASAADDQSLRALLGMFGLLGERAFQDAGTLSGGEKTKL
                     ALAQLVAGRKNLLLLDEPTNNLDPPSRTAIAQALRDWPGAMVIVSHDTEFVEALAPER
                     VLLMPDGGLDYWSADLLDLVALA"
     gene            18465..20129
                     /locus_tag="VF195_03720"
     CDS             18465..20129
                     /locus_tag="VF195_03720"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018171369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6579959.1"
                     /translation="MSSADLAFVNGSVYTVDAARTRASAVGVRDGRIVAVGSDEDVRS
                     ELGATAEVVDLHGRMVLPGFQDAHVHPVSGGVDRLQCDLHDLHSRDAYIQAVRSYAEA
                     HPDREWILGGGWSMDVFPGGTPPKDLLDAVVPDRIVFLPNRDGHSTWVNSRALELAGV
                     TSETPDPADGRIERDPFGEPSGTLHEGAADLVGRLAPAATPDEVYEGLLEGQRYLHSL
                     GITAWQDAIVSDRFWLDNYSAYVRAGENGALTARVIGALWWDRERGEEQIDQLVDLRA
                     RGQAGRFAATSVKIMQDGVCETFTAAVLEPYLDAAGNVTEERGISFVEPEALKGYVTA
                     LDRLGFQVHFHALAERAVRESLDAIEAALDANGPSDHRHHLAHLQVIHPDDVPRFRRL
                     GAVANAQPLWAAHEGQMDDLTIPFLGEPRWRWQYPFGSLVRSGAGLAMGSDWSVSSPD
                     PLEEIHVAVNRVMPPSYLYGNGNADVFLPDERLDLATALAAFTMGSAYVNHLDEVTGS
                     IETGKYADLVVVDRDLFAHPIDEIADARVDATFVEGVPVYERSGAS"
     gene            20264..>21113
                     /locus_tag="VF195_03725"
     CDS             20264..>21113
                     /locus_tag="VF195_03725"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX6579960.1"
                     /translation="MTEPFDMSLVQRGARNRRTPYYEATQRYGPKGFTVYNHMYFPIR
                     FDTFEAEFEALLHGVTVWDVSVERCLEISGPDGFRFAQLLTPRDLSKCAVGQGKYVLV
                     CDSDGGIVNDPVLTRMDENTFWFALASSDALLFARGLKNAYPDLDVTIREADVAPLQV
                     QGPKSKDLMAKLLGPEILDLKYYFWTEAKIGGAPVVVTRTGWTSEIGYEVYLTDTTKG
                     TEVFEAIMEAGQEFGVVPTGPSDIRRIEGAIFNWGADMTYENNPIELGMDRLVAWDLS
                     DDASISI"
BASE COUNT         3503 a         7203 c         7167 g         3240 t
ORIGIN      
        1 tggtcgacct cgtgaacctg gtcgcctcga agctctagtg gcgctccgga cgctcgacga
       61 cctcggcgac gtcgccggct cccgggtctt cgtccgcgtc gacttcaacg tccctctcga
      121 cggcggccgc gtggcggacg acctgcggat ccgtgccgcc atccccacac tccgtgagct
      181 gcgcgcgcgg ggggcagccc tcgtggtggc gtcccacctc ggacgtccgg aggggaaggc
      241 gcgggacgac ctccggctcg cgccagtcgc cgaccggctc ggcgagctgc tcgaggtcga
      301 ggtgatggcg ctcgacgagg tcgtcggcga cgaccccgag gcagtctgcg cccacatcga
      361 tcagggcgac gtggtactcc tcgagaacct ccgcttcgat cctggtgagg agtcgaacga
      421 cgtccgcttc gcgggacgcc tcgccgagct cgccgacgcg tacgtggacg acgcgttcgg
      481 cgccgcgcat cgggcgcacg cgagcgtcgt cgcgctgccc gacctgatgc gagcgtcggg
      541 ccggacgacg gcggccggac ggctgctgca gcgggaggtc gaggtcctcg gccgactcct
      601 ccgaggcccc gaccggccgt acgtcgccgt cctcggcggc gccaaggtct cggacaagct
      661 cggggtgctc ggcgctctgg ccgagcgcgc cgacgcgctg ctgatcgggg gggcgatggc
      721 gttcacgctg atcgcggcgg acgggggcga gatcggcgac agcccggtgg agcgcgatcg
      781 cttcgacgag gtccgtgcgg tcatgcacgc cggcgacgcc ctgatcgagc tcccccaaga
      841 cgtcgtcgcc gccaccgagg cgagcgcgga cgtccgcccg cagaccgtcc cggcccgcga
      901 gatcccgcgc ggcctcaaag gactcgacat cggaccccgg accgtggagg agttcgcccg
      961 gatcctcgcc gacgccaaga cgatcctgtg gaacggtccg atgggcgtct tcgagctcga
     1021 gccgttctcg gcgggcaccc ggggcgtcgc cgtggcgatc gcaggggcgg ccgcgttctc
     1081 ggtggtcggc ggcggggact cactgctcgc ggtcaagcgg gaggggctcg aggattcgtt
     1141 cgatcacctg tcgaccggcg gcggcgcctc gctcgacttc ctcgagggga cgccgctccc
     1201 cggcatcacg gtcctgcagg attgacgtgg gagggctgac gtggccgacc ggacgccgat
     1261 gatcgccgcg aactggaaga tgcacaagac gcacctcgaa tcgatccagg ccgtgcagaa
     1321 gctctcgtac ctgctcgacc ggaaggacgc cgagcgtgtg gaggtcgtga tctgcccagc
     1381 gttcacggcc ctccgttccg tgcagaccct gatcgactcc gaccgcttgc cctacgggtt
     1441 gggcgcgcag gacgtccatc ccgaggacga cggcgcgttc accggggcgg tggcggggcc
     1501 gatgctggcg gcgctcgacg tcgcctacgt catcgtcggt cattccgagc gccgccagct
     1561 gttcggcgag gacgacgcga cggtcaaccg gaaggtgaag gccgtgatcc gcaacgggat
     1621 gacgccgatc gtctgcgtgg gggagaccct cgaggagcgg gagtcgggcg gcacccagac
     1681 gaaggtggcc gaccaggtcc gtcgggcctt cgacgggatc gacccgaccg cggcggccac
     1741 cttcgtcgtc gcatacgagc cgatctgggc gatcggcacg ggccgtaacg ccgaacccac
     1801 cgacgcggga caggtcgtgg aggtgatccg cgccacgctc gcggaccgcg tctcctccga
     1861 ggccgccggc gcggtccgcg tcctctacgg aggcagcgtc aagccgggca acatccgtga
     1921 gttcatggcc cacccggaga ttgacggggc gctggtcggg ggcgcgagcc tcgatcccga
     1981 gagcctggcc ctgatcgccc ggtaccggtg acgcgctttc gcttccttgc ccatcaaaac
     2041 ccctgcgagg gtgcccctca gggggttgct ggattgcggg tcccctgcaa gctctctagt
     2101 atcggttccc gggcatgagt cgatccgaca cggacaccga cgtatcccag gcggaacagc
     2161 gcgaagctgc cgagcgcgcc gacctggagg ccgccgcgcc gtccgccgag accccggcct
     2221 cgatcatcgg ccggagcccc tgggagatct tctggagcca cttccgccag gacaagttcg
     2281 ccctcatggg gctggtgatc atcggggtga tgatcctgat ggcgatcttc gccccgctgg
     2341 tggccagctg gtccgggcac gagccgaacc aggtcaacct ggaggcgctc gacgagttcg
     2401 gcctcccgtc ggcgcccacc tggtggccgg gcccggaggc gaaggcccct gcgaagtacc
     2461 tcctgggggt cgacgacacc ggacgtgacc tcttcgtgcg gatcgcgtat ggcgctcgga
     2521 cctccctgac ggttgcgttc ttcgcgacgg gcatcgccgt ggtgctcggc gtgctcatgg
     2581 gcctcacggc gggcttctac cgtggcaaga tcgacacgat catctctcgc gccaccgacg
     2641 tcgtgctggc gctgccgatc ctgctactcg cgctcggcat cgcgtccgcc tgcggggcga
     2701 atcaggaggg ttgcttctgg ggcttcatca aaccggggct gcgaccggtg atcttgatca
     2761 tcgggctctt cggctggccg tacatcggcc ggatcgtccg cggccaggtg ctgtcgatcc
     2821 gcgagaagga gttcgtggag gcctcccgct cgctgggggc ctccaaccgg cgcatcatcc
     2881 tccgggagat cctcccgaac gtggtagcac cgatcatcgt ctacaccacg ctcatcatcc
     2941 cgagcaacat cctgttcgag gccggactct cgttcctcgg cgtgggggtc ccggacggga
     3001 caccgtcctg gggagccatg ctcgccgacg ccggcacggc cttccgatgg gcgccctggt
     3061 tgatgatctt cccgggcctc gccctgttcc tcacgacgct ggccttcaac ctcgtggggg
     3121 atggcctccg ggacgcactc gatcctcgga aggccgtctg agcgtatcga cagggggaac
     3181 ccggttcgaa aggaggacga cctcgagcac ggagcaccgt tgatcgttgt atctgcacca
     3241 tgagaagggg ggtagctttg aaacgagtat tcagactgat cgcggtcgtg acggtcctcg
     3301 ggatcgtcgc cgccgcatgt ggcggcgacg gaactggcga tacgccgacg gatgagccga
     3361 gccagcctgc ggatctctcg ggtggcacgc tcaccatggc gatgctcgcc gacgtcaccg
     3421 cggcgttcga cccacagaag gagtattact ccgtcacgtg ggagtactac cgctgctgtc
     3481 tgctgcggaa cctgatgtcc tacgagggtg tgccgacgga cgagggcggc gccgacatct
     3541 tcccagacct cgcggtggat gaaccgacac agtcctcgga cggtttgacc tggacgtacc
     3601 agatcaagcc ggggatcaat tacgcacctc cgaaggagga cgtggagatc acggcgcagg
     3661 acttcatccg cgcgctcgag cggacggcga accccgatgc gaacgtcggc gggtactcct
     3721 tctactactc ggtgatcgag ggcttcgacg acttcgccgc gggcgacgcc gaagagatct
     3781 ccggattggc cgcgccggac acgcacacgc tcgagatcac cacgacctca ccggtcggcg
     3841 acatgagcta ccggctggcc atgggcaccg catcgccgat cccgcccgat gacgagggcg
     3901 agcggatggg tgtcgccgag ggacacgacc ggaactacgg tcgattcctc gtggcctccg
     3961 gcccgtacat gttcgagggg tccgagacga tggacttctc ggcggacccg aaggaccaga
     4021 ccgaggcgcc gggctacgtg ccgggtcgcc agatcgtcct ggtccgcaac ccgtcctacg
     4081 atcccgcgac ggacggactg cgggatgcct acccggaccg gatcgaggtt cggatcggcg
     4141 gcgacaacga cgacctgtac aaccaggtcg ccgcggggac cctcgacttc gtggtcgacg
     4201 gcgtcgtgcc cgcggagaag atccgcgagt accagacgga ccccgagctg caggacaaga
     4261 tcaacatcta cgcatcggac gcgatccggt acgtgtcctt caacctggcg gtacctccct
     4321 tcgacgacgt ccacgtccgc aaggcggtca gctgggcgtt cgacaagcaa gggttccggc
     4381 agttgcgggg cggtccctcg gtcggtgaga tcgcggggca catcatggtc aacagcctcg
     4441 agaacaacct cttggcggac tacgacccgt accagacgac gaacgcgtcg ggtgaccccg
     4501 agaaggccaa ggaggagatg gcccagtcca agtacgacac ggacggggac ggggtctgtg
     4561 atgatccgtc gtgccaggac atcctcacga tcaccgatcg cgaggacccg tacccggatc
     4621 agatggccct tctcaagcag ttcctcgagc cgctcgggat gacgctcgac gacaagcagc
     4681 tggagcgcgg cacgatgtac aaccagtgca acgatgcgaa ctcgcatcac gcgctctgcg
     4741 ccggccccgg ttgggggaag gactacgccg acggcgtcac gttcggcgag cccttgttca
     4801 cctccgcggg tctgtgggag agctgctgca actactccct cctgggagcc tcaccggagc
     4861 aattgaccga gtggggctac gacgtgacgg aagtcccgtc catcgatgac aaggaggacg
     4921 agtgcgcggc gttggagccg ggtgacgaac ggttccagtg ctgggccgag ctggaccagc
     4981 agttgatgga agagatcgtt ccgatggtgc cgtacctgtt cgacaacagc gtcgacatcc
     5041 tttcggagaa catcgtcaac tactcgttcg atcagttcgc cggtttggcg gcgttcgact
     5101 cgatggccgt gcccggcaac accggatagc agctgatcca gacggtcgag ggggtcgcgc
     5161 gagcggcccc ctcaccgttc cgtccgaggt acccgcgaag gggcggaggg aggagcagac
     5221 ggatgggccg atacctgatc cggagagccc tcttcatgat cctcgtgatc atcatcgtct
     5281 cgctcctgac gttcctcatc ttcgtgaagc tcccggcagg ggatccggct cgccgggcgg
     5341 tcggtcgtac caccactccc caacagatcg agaacgcgcg cagggcgttc ggtctggaca
     5401 agccgctcta catccagtac tggcggttcc tccgggggtt catcccgtgg cctccgggcg
     5461 atccggtcct catgttgagc ggccaccccc cgcggagcca tgaaggcctc tggctcaacg
     5521 aggacgtcta ctactcttac gatggcttcg tcccggtccg agaggagctg gcccggcgga
     5581 tccccatcac cgggaccctc gcgatcggcg ccgccgtgat ctggctgctc ctcggcatcc
     5641 cgatcggcat cctgtccggc gttcggcgaa gatccgtcgc cgaccgcgcc gccatgatct
     5701 tcgcgatcgt cggcgtctcg atgccgatct tctggttggc gcagctcctc ttgtacgcgt
     5761 tctggttcaa gctcgagctg ttacccgaat ccgggatccc gatcgggtac agcggctggg
     5821 gcggggcgat gcgcgccgcc ctcgagggcc ggttcatcct cccctgggtc tccctcgcgt
     5881 acatcaacgc ggccttctac gctcgcatgg tgcgcggcaa cctgatcgag acgatgaacg
     5941 aggactacat ccggacggca cgagccaagg gcctgtcgga gcgtcgcgtg atctacaagc
     6001 acggcctccg cggggccctc acgccggtcg tgacgatgct cggcctcgac gtcggtctcc
     6061 tgctcggcgg cgcgttcatc accgagacgg tcttcgggct cccggggatc gggcagaaag
     6121 ccgtccaggc gatcggcacg aacgacttcc cgaccgtgat gggcgtcacg atcctcggcg
     6181 cgttcatcat cgcggtcgcg aacctggtgg tcgacgtagc gtacgcgttc gtggacccga
     6241 gggtgaggta cacctaggtg gcgcagccgc tgctcgaagt gaaggacctc aaggtccatt
     6301 tcccgaccga cgacggactc gtcaaggccg tggacggggt gagctactca ctcgcggcgg
     6361 gggagacgct ggggatcgtg ggagagtcgg gctcgggcaa gagcgtgagc tccctcacgg
     6421 tgatgggcct gatcaatccc aagcaagcga ccatctccgg cgaggtcttc ttccagggtc
     6481 aggacctcct gaagcttccg aaggacgaga tgcgcacgat ccggggtgcg aagatctcga
     6541 tgatcttcca ggacccgatg acgtcgctcc atccgttcta caaggtcggc tatcagatcg
     6601 ccgaggcgat cctcgagcac caggacatct cgaagaagga ggcccacgac caggccgtcg
     6661 agatgctcgc ccgggtgaac atcccgcacc cgaaggaccg cgccgaccag tatccgcacg
     6721 agttctccgg cggcatgcgc cagcgggcga tgatcgcgat ggcactgtcc ctcaaccccg
     6781 acgtgctgat cgccgacgag cccaccacgg ccctcgacgt cacggtgcag gcgcagatcc
     6841 tcgatctcat cgatcgcctg aagtccgagt tcaacgccgc ggtcgtgatc atcacgcacg
     6901 acctgggcgt ggtcgcggag cactgcgaca acatcatggt gatgtacgcc ggcaaggcgg
     6961 ccgagttcgg cgacaccgac gacatctatt acaaaccgct ccacccgtac acctggggac
     7021 tgctgagctc gatccctcgg ctggacgagg agcagcagga gcggctgaac ccgatccgcg
     7081 ggctgccgcc gtcgctgatc caggtcccgc ccgggtgcgc gttccatccg cgctgtccct
     7141 accgattcga tccctgcgat cgcgacgtcc ccgagctcct cccggtggac ggacatcacg
     7201 cggccgcgtg ccacctcagc ctcgcggaca aggaacggat ccgcgaccag gagatcctcg
     7261 ctcaccgatg agcgtccagg cgcagcccgt ccccgcgcag ccgccggctc cctccggcga
     7321 gccgctcgtg aagatcgagg gggtcaagaa gtacttcccg atcacgcagg gcatcctctt
     7381 ccagcatcac gtcggcaacg tgcacgccgt cgacggcgtg gacctcgaga tctacccggg
     7441 gcagaccgtc ggcctcgtcg gggagacggg gtgcggcaag tccaccctcg cgcgcgtcgt
     7501 gatgcgcctc tacgacgcga cggaagggac gatctggttc caggggcaag acatcaccca
     7561 tctcaagggc aaggagctcc aggagctccg cgaagacatg cagatggtgt tccaggatcc
     7621 gtacgcctcg ctcaacccca ggaagaccgt cggcacgatc gtgtcggagc cgttccgtct
     7681 gcaccagacc gtgcccaaga accggatcaa gtccgaggtc cagcagctga tggagctcgt
     7741 cgggctcaac ccggagcact acaaccgcta cccgcacgaa ttctccggcg gccagcgaca
     7801 gcggatcggg gtggcgaggt ccctcgcgct ccgcccgaag ctgatcgtgt gcgacgagcc
     7861 cgtctccgcg ctcgacgtct cgatccaggc ccagatcctc aacctgctcg gcgacctgca
     7921 ggacgagttc aacctgacct acctcttcat cgcccacgac ctgtccgtcg tcaagcacgt
     7981 ctcggaccgc gtggccgtga tgtacctcgg caagatcgtc gagatggccg agggcccggc
     8041 gctctacaag acaccgaagc acccctactc gggagctctc ctgtccgccg tgccgatggc
     8101 tgaccccgag ctggcgcggc agaagcggcg gatcatcctc cagggagacg tgccgtcgcc
     8161 gatcgatccg ccgtcgggat gccggttcca ccctcgatgc ccgcagtccc gggcgatcgc
     8221 gaaagccgag gggatcgacg gaccgcaccc tcgctgcgtc caggaagagc cggagctgtc
     8281 ggaccagggc gccggccagg tcgccgcctg ccatttcccg ttggagggaa gtatcatcga
     8341 gggcggcgac gcggaagcac ggttcgagga cggcgtcgtc gagatgcagg aggggttgtc
     8401 cggcagcgag gacaccccac aggacggacc gtaggccacg ggttcgcagg ctcacggcgc
     8461 cgggaggaac gtgtcgtgct gaacgcagtc gtgatcgtga tcaacatcat cgcgtcgctg
     8521 ttcctgatcc tgtcgatcct gttgcactcg gggcggggga gcgggatgtc ggagctcttc
     8581 ggcggatcgt ccaccctcgc gggcgggacg gccctcgagc ggaccctgga tcgggtgacc
     8641 gtcgtcacgg cgatcgtgtg gggcctgtgc acgttctggc tcgcctggaa gtggaccccc
     8701 ggctcatagg gcttgatagc atccaccgcg acctcgtcgg agtggcggaa cttggtagac
     8761 gcgctaggtt gagggcctag tccccttcac agggggtggg ggttcaaatc ccccctccga
     8821 cactcgagct acctgcaaga acgcccgcgg cctggacagc ttctacggcc ctgatgacgg
     8881 tgagtggcta cgcgtgggtc gagtgcgctg cctgcgacca cggctggctg gttccggact
     8941 cgccgcggag aggaggaacg gcgacgaccc ttcggccgcg cccgtccgac gacgagatcc
     9001 cgggtacgag gtcaccgacg tgcacggtca gctgatccac aagggtggag cctggccgaa
     9061 gccatcaacc tgtacgccgg cacccgcgct ccacgcctcg tcgggagctc gcttgcggca
     9121 ccgccgtcat gcgctcctca tcgtcggcgc cgcgtgctga cgaccggtta ggtgcccgcc
     9181 gttcgcccac gccactgccg cgaccatcat cgagaacacc gccccccaga tcagcaccgg
     9241 ggtcacgagc acgatgcccg acaccccttc ggaagggaca gagatcgcca cgaacgcggt
     9301 cgcgggcacg tgcatcaagt acatcaacag cacgctctgg gtccggtcgt agacccacac
     9361 catgagcacc ctgtacggga tcagccacgc gaacagcagc gccaccacca gaagagcgga
     9421 cggcacgacg ccttccggat ccgtgtcacc cgcgaagagc gggaagtgcc acgcgcccca
     9481 cagcacgccc atcaccagcc ccgtcgtgag cacgctgtag cgccgcctca gcctgggcag
     9541 ggcgaatccg gtccagccga gctcttcgag gaatggaccc acgagccctg ctgcgatccc
     9601 gatcaccacg atccccagct tgtcgtcggc ggtgacgatg tcgggacgga actccggtga
     9661 tgcgagggac agggcgaaca ccgtcgcgcc catgacgagc ggggcggtga ggagggctac
     9721 cccgtaccaa cgcgcgccga cccgccatcg ccgcagtcgc gacccgagct cgcgaagacc
     9781 ggccctcccg tcgacgagag ccgtcaggag cagactcgcg accggggggc cgatgatgac
     9841 caggacgatg gcggccgagg ccgtcgaacc cgtgctgaac aacccgtcag gacccaggac
     9901 gaggaggatg cctcccaacg agatcgtgaa tacaagcacg tagtacgctg agagggcgtg
     9961 ccgcttgatg aagccgaggc gggggctcgc cgccggcccc tcgggacgct gcagcttggg
    10021 cggccgttcg agagtggtgg acactgtgca cctccctggg cgcgggcttg ccgatcgcgc
    10081 cctcaggctc atcgtcctgc cacggagcgt cgccgagaag gggagatagc ctcgggtccc
    10141 ttgtgacctt cggcccggga acagcgggcg ggtggcgtgg agcggcctcg ccttccccct
    10201 ccgtggtggc gatgccgccc acgacgctcg aagaggaccg gaagcaaacg gggatggtcg
    10261 attcgggcaa gcggcgcggc gcctcggcat ctccgtgcgc gagcaccggg agcttgaggc
    10321 cggcgagcgc atgccgaact tggagacgtt cgaccgaatc tgtactgatg gccgctgaag
    10381 taggcacttc gtaacttcac aggcctcggg ccgggcagtg atgcccccgc cctacctgtt
    10441 gccctcttgg ccggcggcgg attgggcgtt cccctgcctc agtggcgacg ctctccgtca
    10501 ggctcgagca cggtcggaag catggcggtt agacgcgtag cctggaggct ctcgcgcccg
    10561 gctgcgtcag cctggcttgg ccacggttag caggatggcc gcgtcttcca acgcctcgag
    10621 cttgtggcgt gcgtccggta cgatgagcac cgttcctgtg cgaccctccc aggacgaatc
    10681 tccggccgat atccggacgc gtccatgcaa gacctgcacc gtcgcctcgc ccgggctctc
    10741 gtgctcattg tggacgacgc ctttcatcat ggcgataacc gtctgacgca gcaccttcgc
    10801 atgcccaccg acgatcgttt ctgcgctccg gccgctctcg gtgtttgcag ctcgcttgag
    10861 ctcttggcga gcgagagagt cgagtgagag cttctgcacg tttccattct tctcttcccg
    10921 acggagcagg gcaaacgaag gatgatcctg aacccgacgg aaagtcatgt gcaagcgctg
    10981 ctccccgaga tgctcgagca ggatcggaag cgagcgggct ggtcagcccg ggaagcggcc
    11041 tgcggctcgg tgagcgtccg agagtccggg agctcgaggt aggcgacgct gggccactgg
    11101 caggcgttcc accggatctg cgagctctac ggctcgcggc ggacgtacgt cgccgctcgt
    11161 cctccgcctg tcaggttgat cgccccgcta ccgatagagc accactactc cgacggtcca
    11221 gcaagccaag gccaccggat cgacttccac cgatagccgg tagccctctc gggctcgagg
    11281 gagatcgtga cacgcatggg acgcgcgagt ggactcgagc cgggtccaac cgttcgtcca
    11341 acgaactggc agaaatcgcc ccacaccgcc gcacactgga cgaaccagga tggggatgag
    11401 cagcatggaa gaggactcag ccacacgttg gatcactgaa cggtgtgagg cgtaatcccc
    11461 tcacaggggt gggggttcaa ctcccccctc cgacacgttc atctcccgga ctccacggcc
    11521 atcggctcgg tctcctgctg tgtgacgtgg agcgggacgc tcaccacgac aacaccgggc
    11581 tcgaacagca acaggcgctt gaagaacaac gcgctctggc tgtggagcac ctgttcccac
    11641 cagtgtgaga ccacgacctc cgggaggacg acgctcacga tcgtgccctc tctcgacgtg
    11701 taccggcgca cctcctcgag catcggctcc gcgagatcgc ggaacggtgc atcgacgatc
    11761 gcgagcggga tgctcatccg gtgatcgatc cacgccgccg cgagctcgcg ctcgtcccca
    11821 cgatccgtcg agaagaagag cgctgcgacc tcggtggcct cgagggagag ggcgtacgag
    11881 accgcctcca ccgacgcagc gtgcacggcc gagacgggga ccagggtcac gtgccgactc
    11941 ggttccaggg gcggggtcgc gtctcgagcg gcctcctccg ggaggaacgt gacgtcggcg
    12001 accacgatgc ccgcctcgaa gaagagggtg accttcagcc agaaccccca tcggcggccg
    12061 aggtggcgca ggagcgagcg gctccggatc tcctcggcca ccaccacggt gacgaacccc
    12121 tcggcatcct tccggagcga tcgcagccgc gcgcgaagag cgggcatcgg gcgaccgtcg
    12181 gccgcgtcca gcagctggag agctccgagt cggggggcga gcgccggcca gcgctcggcg
    12241 gcgctctcga atgcctccgg atcgccgacg aacagcggcg ttacatgctc cggtcgcagc
    12301 gcgcgcacgt atccaacggc cttggccgtc gccggaccga ggtcccggac gagcatcacg
    12361 aacgtgttcg gtgccccgcg tatcgggggg cgcgtgcgta ggcggagcgc ccgcccgatc
    12421 gagccgtagt gccggtgcac cgcgaggaag aacgcgatga tcaccggcat cgcggcgagg
    12481 acgatccatg cacctccgct gaacttcgtg atcgtcacga tgacgaggac cacccccgtc
    12541 gccgtcgcgc cgatcgcatt gatgatcatg ctccgccgcc acccgggcgc ccgcagccgc
    12601 aaccagcgcc ggaccatccc cagctgggag agcgtgaacg ccgtgaagac gccgacgacg
    12661 tacagctgga tgagcttcgt gagatcggcg tcgaacacgt agacgagccc gctcgccgcg
    12721 agcgcgagga tgatgatccc gttcgagaag acgagccgat caccacggtt gcggaactgc
    12781 gaaggcatga agcggtcgcg ggcgaggatc gcggagagtc tcgggaagtc ctggtaggcc
    12841 gtgttcgcag cgaggatcag gatccctgcg gtgagtcctt gcagcacgaa gtagaggaac
    12901 gtccggccga agacggtctc tccgatctgg gacagcaccg agcgggatgc cgcgatctca
    12961 tgactcaccc gtacgtcgag cagtgtcgtg aggaccgtga tcccgaggaa catcgatacg
    13021 gtcatgacgg ccatcatcga gagcgtcctg gccgcgttct tggcctgcgg ccgccggaaa
    13081 gcggggacac cgttgctgat cgcctcgacc cccgtgagcg cggtcgctcc ggatgagaag
    13141 gccttcagga ccaggaacag gctgagcgga gccgtggcct cgagcgggag gtgcgccgtc
    13201 gccgcctgag gacaaccgcc caggcatcgg aggaacccga cgccgagaag cacgtacacg
    13261 atcgcgacga acccgtaggt cgggatggcg aacaggaccc cggcctcctt gacaccgcgg
    13321 aggttcgcca acgtcaccag tacgacgagg ccgatcgaca ggatgacctt gtgtccggcg
    13381 agggatggaa cggcagaggt gatcgccagg aatccagcga cgatcgacac ggccacggtc
    13441 agcacgtagt ccgtgagcaa ggccgcagcc gccaccagcc ccggcactgt ccccagattg
    13501 tcgtgcgcca cgatgtacga gccaccccca ccgggatagg cgcgcaccgt ctgccgatag
    13561 gaggtgacca cgatcaccag cacgatcgcg atcgcgatcg cgatcgggac ccggaacgcc
    13621 agcgcccccg ctcccgccgc caccagaacg agcatcatct cctcggtcgc gtacgcgttc
    13681 gacgacaatg cgtcggagga gaacaccggc agcgcgaggg tcttggggag gagctgatgc
    13741 tccgccttgt ggctcgcgag gggacgtccg acgaggacgc gcttcagcga cgcgttcgcc
    13801 atgtcggctc ggtcgcgtca tcgagctccg cgtcatctag ctcagcgtca tcgagctccg
    13861 agagcgctct ccagacatcg ggcccggcgt cgcgttcgat ccggtcgagc agccgatccg
    13921 tgaggccggc ctcgcgggag gcgtcgaccc actcccggtg ggtctgccac gctcggaacc
    13981 cggggccgat caggatgccc agacagcaac cgatcaggat cccgacgagg agatctccgc
    14041 ctccgcctgc cgccagtatc gagagccagt tcatggcgca agcgtgcatc tcggggcata
    14101 gcgatggagt agggaacgga cgtcccgtcg taaggtttct gtaagggtcg ccgaggctcg
    14161 agagccacct tgctaccctc cggcgggagg ctgtggctga tgcgcgtgag ggatcgtcgg
    14221 aggtggccac gcgtcctcgg tcgagccatg acggccctcg ccccgcccgg tctcgtcacc
    14281 ctcggagcgg tgctcccggt cgacatcccg acgacgacgg ccgccctgtg ctacgtcctc
    14341 gcggtggtcg tcgccaccgc cacgggcggc ctggtggcgg gactcgtctc ctcggtggct
    14401 tccttcctgg cgctgaactt cttcttcacg cctccgttcc acaccttcgc ggtggaggag
    14461 acggccgatc tcgtcgccct cgccgtgttc ctcgccgtgt cggtgaccgt cgggacgatg
    14521 ttctcccgtg ccctcgagca acgcgcgcga gcggagcggc gcgagcgcga ggcacggctc
    14581 ctcaaccatc tgggaacacg cctccgctcc ggcgtgccga cggaggacgt gctccggagt
    14641 ctggcccggt cgatcctgga gctcttcgac ctcgcccggt gcgagatcag cagcgagctc
    14701 tccggacgtc cgatcgtggc cgagaacccc gaccggacga gcaccggcgg acgcgaagag
    14761 gtgatgccca tgaccgtgca ggaccgcgag ctggggcgca tcctcgtggt cgtgggcgac
    14821 ggacgtcccg cgatgacggc cgaggagcga ggcgtcctcc agacgctcgc atcccagatg
    14881 gcgctcgcga tggacggcat gcgactcggg tcggaggcgg aggaggcacg gatggaggcg
    14941 gagacgaacc ggctccgcgc ggcgctcttc tcgtccgtca cgcatgacct ccggaccccg
    15001 ctcgcgtcga tcaccgcatc cgtctccacc ctcctcgagg accggtcgcc gctccacgat
    15061 gaccagcggc gagagctcct ggagacgatc gatcaggaga cgggccgtct caaccgggtg
    15121 atcggcaacc tgatggatct ctcgaggatg cgcgcaggtg cggtgatgcc gacgaagtcg
    15181 ccggcggcga tcgaggaggt gatcgagagc gtgctcgctc gctcggggcc gatcctcgag
    15241 cggcacgacc tccggctcat gatccgagag gaccttcccg agatccccct cgatgtggtg
    15301 ctgatcgatc aagccctgac gaacatcgtg gagaacgcgg cccggttcac gcctcccggg
    15361 agacggatca cgatcggggc ggcgaggtgg cgcgatgggg tccaggtgcg gatcgcggat
    15421 cgcggaccgg gaatcccccg tgaggagcgt gaccgcgtct tcgagccgtt cgtgcgcggc
    15481 gaggggagca cgggaaccgg cctcggcctg gccatcgcac gagcgatcgt cgaggcgcat
    15541 ggcgggtcga tccggatcgg cgaggaaccg ggcggaggga ccgccgtggt actcgagctc
    15601 ccgggagggt cgacgtgaag gaggggcgcg tgctcgtggt cgacgatgac ccccagatcc
    15661 tccgcgccgt acgcacgagc ctgctgggac acgactacga ggtcctgacg gccggcaacg
    15721 gggagaccgc gctgcacctg cttcccgacg cggacgtaga cctcgtcgtg ctggacctgg
    15781 gccttccggg catcggcggc gacgaggtga tccgccgcct gcgctcgtgg tccgacgtgc
    15841 cgatcatcgt gctctccgtc cgtgagggac agcacgacaa ggtgggtgcc ttcgaggcgg
    15901 gggccgacga ctacgtcacg aagccgttcg ccatgccgga gctcctggcg aggatgcgtg
    15961 ccgtccgccg gcgcgtcgaa ggggagcgcc gcccgtccgt catccgcttc gacgacctcg
    16021 aggtcgacct cggccggcag ttggtgagga tcggggggac cgatgtccac ctgaccccga
    16081 ccgagtaccg gctcctcgag gcgttcgtca cgaacccggg caagctgctc acgcatcggt
    16141 ggctgctcca gcgggcgtgg gggtcgggct acgcgacgga gcaccagtac ctgcgcgtgt
    16201 tcatccgcca gctccgggcg aagctctcgg acgatcccgc gcgtccccga ttcatcgtca
    16261 ccgaggccgg cctcggctat cgatggaagc cggatccgga ccttgatccg ccacccgccg
    16321 tatcgtgacc cggtgcccat catcgagatc cacgggctcc cgcccgatag agggctcgac
    16381 gttcccaccg ccctcgcgga cgtgacgagc gcggtcgccg cgtacctggg cgaggagccc
    16441 cggggtacgt gggcgatctg gcgccccgtg gagccgggag cgtacgcgga gggcgcggac
    16501 gctccctcca cgcagccccg cggaacgcat cccgcgatcg tcgacgtgtt cgccggccgc
    16561 cgcgacgaca cgcccgggct cctgcacgcg gtcgggcggg ccgtcgtcga ggccttcggc
    16621 ctcgacgagg gcaacgtcgt cgtccgcctc accgaggccg agcccggacg tgtgtactgg
    16681 ggcgactgag cgctacactc cgcacgtgag ctagcggaac ctcctcgagc agaccgggcg
    16741 gatccatcga gcccgcgtcg tcccacgtcc ggtccccaag gacgtcgccc aaggaggagc
    16801 cgatgcccgt cccacccacg acccgacacc ctcgatcccc gggagcatcc ccatgaacgc
    16861 cctcgacgtg cgcggcctct ccgtcgacgc cggcggtctc accgtcgtac gcgacctcga
    16921 cctcgccatc tcgagcgggg acaaggtcgg cgtcgtcggc cgcaacgggg caggcaagac
    16981 gagcacgctc aaggtcctgg ccggtgagat gccgccatcc gaggggacgg tggaacgatg
    17041 tggggcgctc ggctacctcc ggcaggatcc acggcaggac cgatcggacg actcggcgtg
    17101 cgggatcgag tacctgctcg ccgcccgcgg cctcgtcgag ctgcagcgtg gggtggagaa
    17161 ggcacggctc cagctggagg agcacgcgtc cgaggatcgg gtcgcgcgct tcgcgcgcct
    17221 cgaggaagag tacgaccggc tcggcgggta ccgggccgag tcggacgccc gcacgatcgt
    17281 cgccggtctc gggctgtccc aggatcgact cggcctgccg gtgggcaccc tctcgggtgg
    17341 tgagcgacgc cggctcgagc tcgcccgcat cctcttcggc ggttccgacc tcctcctgct
    17401 ggacgagccg acgaaccacc tcgacgctga tgcgaagggt tggctgatgc ggttcctcgc
    17461 aggctatcgg ggagccctcc tcgtcgtgag tcacgacatc gcgctgctcg acgcctcgat
    17521 cacccggatc ctccatctgg accgcgacgg cgtcgtcgag taccgcggca cctacacgca
    17581 gtatcgggaa gcgagggcac gcgacgagaa gcgcctcacc gcgctcgcct cccgccaaga
    17641 gaccgagatc aagcgactga agacgctcgc cgactcgatg cgtggccaga cggcgaagcg
    17701 ggcgcggaag gcgaagacgc tcgacacccg ggtcgcccga ttgcaggcga ggaaggtcgc
    17761 cgcacccacc cgcgagcgcg gcgtccgatt ccggttcccc gagcccccgc actcaggtcg
    17821 cacgatgctc tccgtggacg gcctcacgaa gggctacggc ggccccgccg tcttcgagga
    17881 tgtgtcgttc gaagtggagc gaggcgaacg gctcctcgtg atgggcctca acggggccgg
    17941 gaagacgagc ctcctcagga tcctcgcggg ggagacccgc gccgacgcgg ggacgttccg
    18001 cctcggccac ggtgcccagc tcggttacta cgcccaggag cacgaaggga tccgcgagcg
    18061 gatgacggtc ctgagccaca tgcaccaagc atcggccgcc gatgaccagt cgctccgtgc
    18121 cctcctgggg atgttcggtc tcctggggga gcgggcgttc caggacgccg ggaccctgtc
    18181 cggcggcgag aagacgaagc tggcgctcgc ccagctcgtg gccggtcgga agaacctctt
    18241 gctcctcgac gagccgacga acaacctcga cccaccgtcc cgaaccgcga tcgcgcaggc
    18301 gctccgggac tggccgggag cgatggtgat cgtgagccac gacaccgagt tcgtcgaagc
    18361 ccttgccccg gagcgtgtcc tgttgatgcc ggacgggggc ctggactact ggagcgcaga
    18421 tctcctcgac ctcgtcgcgc tcgcgtgacc gctatgctgc gccgatgagc tcggccgacc
    18481 tcgccttcgt gaacggctcc gtgtacacgg tggatgcggc tcgcacccgg gcgagcgcgg
    18541 tcggcgtccg ggacggccgg atcgtcgccg tcggcagcga cgaagacgtc cgcagcgagc
    18601 tcggcgcgac ggcggaggtc gtggacctcc acgggcgcat ggtgctcccc gggttccagg
    18661 atgcccacgt ccacccggtg agcggcgggg tcgatcgttt gcagtgcgat ctgcacgacc
    18721 tccattcccg cgacgcctac atccaggcgg tccgctcgta cgcggaggcc catccggatc
    18781 gagagtggat cctcggtggc ggctggtcga tggacgtgtt ccccggcggc accccaccca
    18841 aggacctcct cgatgcggtc gtgcccgacc ggatcgtgtt cctgccgaac cgggacggtc
    18901 acagcacgtg ggtcaacagc agggcactcg agctcgccgg cgtcacttcg gagacgcccg
    18961 atccagccga cggccggatc gagcgggatc ccttcggcga gcccagcggc accctccacg
    19021 agggggccgc cgatctcgtt ggccgcctgg cgccggctgc cacgccggac gaggtctacg
    19081 aagggctcct ggagggacag cgctacctcc actccctcgg gatcaccgca tggcaggacg
    19141 cgatcgtgtc cgaccggttc tggttggaca actactccgc ctacgtccgg gcgggggaga
    19201 acggggcgct gaccgcccgc gtgatcgggg cgctgtggtg ggaccgggag cggggcgagg
    19261 aacagatcga ccagctcgtc gacctccgcg cgcgtggcca ggcgggtcgc ttcgccgcga
    19321 cgagcgtgaa gatcatgcag gacggggtgt gcgagacgtt cacggccgcc gtcctcgagc
    19381 catacctcga cgcggccggc aacgtgacgg aggagcgcgg gatctccttc gtggaacccg
    19441 aagcgctgaa gggctacgtg accgcgttgg atcgactggg cttccaggtc cacttccacg
    19501 cgctggccga gcgagccgtg cgggaatcac tcgacgcgat cgaagccgcg ttggatgcga
    19561 acggcccgag cgaccatcgc catcacctcg cccacctgca ggtgatccac cccgacgacg
    19621 tcccgcggtt ccgtcgcctg ggggcggtgg cgaacgccca gcccctgtgg gcggcgcacg
    19681 aaggacagat ggacgacctg acgatcccgt tcctggggga gccccgctgg cggtggcagt
    19741 accccttcgg ctccctggtc cggtccggtg ccgggctggc catgggcagc gactggtcgg
    19801 tctcgagccc cgacccgctc gaggagatcc acgtcgcggt gaaccgtgtg atgccccctt
    19861 cctacctcta cgggaacggg aacgccgacg tcttcctccc ggacgagcgc ctcgacctcg
    19921 ccaccgcgct cgcggccttc acgatggggt cggcgtacgt gaaccacctc gatgaggtca
    19981 ccggctcgat cgaaacgggg aagtacgccg acctcgtggt ggtagatcgc gatctgttcg
    20041 cccacccgat cgacgagatc gcggacgctc gggtcgacgc caccttcgtc gagggggtcc
    20101 cggtgtacga gcgttccggc gcgagctgac ttccggtcac cccgcctggt tgcctggccc
    20161 ggacggcgct cgcacgatag cgaagcatcc ctgcggacgg gatggcgcag acggaaccgc
    20221 cggcgacggg caggcggccc gcccttcgag aggagccacc acgatgaccg agcccttcga
    20281 catgagcctc gtccagcgcg gagcgcgcaa ccgccggaca ccgtactacg aggcgaccca
    20341 gcggtacggt ccgaagggct tcaccgtcta caaccacatg tacttcccga tccgcttcga
    20401 cacgttcgag gcggagttcg aggcactgtt gcacggggtc acggtgtggg acgtctcggt
    20461 cgagcgctgc ctcgagatct ccggaccgga cggcttccgg ttcgcgcagc tcctgacgcc
    20521 gcgcgacctc tcgaagtgcg cggtgggtca aggcaagtac gtgctggtct gcgacagcga
    20581 cggtggcatc gtgaacgatc ccgtcctgac ccgcatggac gagaacacgt tctggttcgc
    20641 gctggcgtcc tcggatgccc tgctgttcgc gcggggcctc aagaacgcgt accccgacct
    20701 cgacgtgacg atccgtgagg ccgacgtcgc accgctgcag gtccaggggc cgaagtcgaa
    20761 ggatctgatg gcgaagctcc tcggcccgga gatcctcgac ctgaagtact acttctggac
    20821 cgaggcgaag atcggcggcg cacccgtcgt ggtcacgcgc accgggtgga cgtccgagat
    20881 cggctacgag gtctacctga ccgacacgac caagggcacg gaggtcttcg aggcgatcat
    20941 ggaggctggt caggagttcg gtgtcgtccc caccggcccg agcgacatcc gcaggatcga
    21001 gggcgcgatc ttcaactggg gtgccgacat gacgtacgag aacaacccga tcgagctggg
    21061 gatggatcgg ctcgtcgcat gggatctgtc ggacgacgcc tcgatctcca tcg
//
LOCUS       DASUYD010000067        22684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_915825, whole genome shotgun sequence.
ACCESSION   DASUYD010000067 DASUYD010000000
VERSION     DASUYD010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 22684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22684
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_915825"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF195_03730"
     CDS             complement(<1..369)
                     /locus_tag="VF195_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX6579961.1"
                     /translation="MRRPPAGRLVALLGVVVLALAGVGTRLVVLQVGDHRAFAEQGLD
                     QRVHLIDLPAERGAIVDRTGVPLAITLEARDVYADPRYVTDPIGEAARIARILDLQPR
                     QVERLLRTPDSTFVYLGRQVD"
     gene            complement(366..818)
                     /locus_tag="VF195_03735"
     CDS             complement(366..818)
                     /locus_tag="VF195_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579962.1"
                     /translation="MSLPAREADRWAPAPQQRPRRRAVSPARPPATHPPIPTPPHPHR
                     RARRGHHTAFWVLTAVVVSSLVVGLVSVNAMRVDAAYRSRSLVEEIRTMTDERRTLVN
                     DVAHLSSPSRIGRWAVRQGLVHPAAGDVVILQVPGFSRPTTPTVEAGE"
     gene            complement(815..1693)
                     /gene="rsmH"
                     /locus_tag="VF195_03740"
     CDS             complement(815..1693)
                     /gene="rsmH"
                     /locus_tag="VF195_03740"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011529909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEX6579963.1"
                     /translation="MRPPHEPVMVAEVVECLLPGGSGTGRRVADMTVGAGGHAAALLD
                     AGVERVIGFDRDPEALRVARERLAPYGDRFTAVHRRFSQVGEAEATGPLHGVLFDLGV
                     SSMQLDEPARGFGYRVDGPLDMRMGEGDVTAADLVNDLPESELADLLFEYGQEHRSRR
                     VAAAIGRARQRARIETTDQMAGVVASALGRRPGGPHPARRTFQALRIAVNRELEELAT
                     SLPRAVGLLGPGGRVVVLSYHSLEDGMVKRAFRDDERLRVLTKKPLQPSAEERSRNPR
                     ARSAKFRAAERVEEAA"
     gene            complement(1847..2335)
                     /gene="mraZ"
                     /locus_tag="VF195_03745"
     CDS             complement(1847..2335)
                     /gene="mraZ"
                     /locus_tag="VF195_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEX6579964.1"
                     /translation="MAELLGTHSYSLDPKGRVSLPARFRAAFDEGLWLTVGQDRCLYC
                     FPRAEWERRSREVGAFALSDKDGRAFSRLFFSAADEVRLDAQGRVTIPQRLRDAVGIN
                     KEVVVLGVRERIEIWDRAEFERYRDAHADAYENGDLEPRGARPVAGGHPGFDRAPRTT
                     EG"
     gene            complement(2479..2964)
                     /locus_tag="VF195_03750"
     CDS             complement(2479..2964)
                     /locus_tag="VF195_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX6579965.1"
                     /translation="MGRALVLNASESPLAVVAARRAVVMVLKEKADVILSNGLVFHSA
                     RLTIEAPSVVRLRRYVHVPYRPHAPLTRRAVFARDLGTCQYCGAAAENLDHVIPRSRG
                     GLHTWDNVVASCRKCNARKEDRTPHESGFSLRRQPYAPRDGFHLTLGRIEPGWELYLA
                     S"
     gene            3285..4259
                     /locus_tag="VF195_03755"
     CDS             3285..4259
                     /locus_tag="VF195_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014688717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6579966.1"
                     /translation="MEVREKIPDPRAFAARFREIEENVEQVVRGKHEEIRLALVALVA
                     EGHLLIEDVPGVGKTMLAKSIARSIDCSFRRIQFTPDLLPTDVTGVNVFSQETGDFEF
                     KPGAIFANIVLGDEINRASPKTQSALLECMEERQVTVDTETHTLGTPFIVIATQNPIE
                     HEGTYPLPEAQLDRFMMRLAIGYPDAEVAAEILASHAAGAPLDDIRPVSDASGVREMI
                     EEARRIHAAPPIRRYIVDLVEATRRHSDVYLGASPRASIMILRAARALAAAEERDYVI
                     PDDVKTLAPVALPHRIIVTADAIMSGRSAEVLVREIIDEVDVPIAENP"
     gene            4276..5505
                     /locus_tag="VF195_03760"
     CDS             4276..5505
                     /locus_tag="VF195_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX6579967.1"
                     /translation="MLAGRGVAVFVTGIAMWLAARFLGSPGLEVIAIGIAALPLIASF
                     AAKRGAQRISVRRRLSDVRISPGSRVTVTLDVENRAAIGTSFLLLEDRLPTPLGRAAR
                     LVIGGIRAGKREEATYTLVPQSRGRYLLGPLVIDVSDPFALSRVRLEFDDREELLVAP
                     EIEDLGAAPDPASGPSFGASRARQLFRTGQEYYTMRSYQEGDDLRRIHWPSVARTGEL
                     MIRQDESTRRASGLVFLDTRAAALGRTQTPAFERGVSATASVGVLLARRGFVLRLGTT
                     ELPAAGLSEERFLDALTGISHSEVRSIGPALAHLRSGSSPDTSLVFVSAPPVPAELSA
                     LIRAGAGFGQKLAILIYPTDPVRLPPERQAQLEGRATQARLSLARAGWDVLVLPPSMR
                     LQERWHTPVERPLARIV"
     gene            5463..7706
                     /locus_tag="VF195_03765"
     CDS             5463..7706
                     /locus_tag="VF195_03765"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX6579968.1"
                     /translation="MAHAGGTSARAHRLIALAAAALLAVTTALAFGRVFLGASSTLSL
                     VAAAVGAALLAAAFERRSLLLATFVSSAGLVLAIGLIVFPQTTWYGLPTAETLRAALD
                     AAALVGEQARIQPAPAEPIDPLLLAAVVSLWAAVFSAHALAFRAGSPLLGLVPPVALV
                     AFADTVLEEFVKPLYGVAFLAAALLVVFADGLARVQGWGPVWASGRRGIAATAGRGAR
                     RLAFTALGAALLSPILVPGFGSKGLVDFGTPAADRVAIDPFVSVESALTQTEPVEVLR
                     VTTNHPGYIRLVTVPEFDGVGWRPAEMSEGVPLVPGAELLDPGRGEKTTARIEIMRDL
                     GQRYLPAPSPIGSIDGIADRTVRYDRDLWNAFVDRPLSEGDIYEVTTYRPQVTAEALR
                     NIPFFNTLEDGRYTDVPDDLDERIRAIALDWTEDADNVFDKVVAIEERLHSSGEFGYQ
                     VDTPLRSGSSSILEFLTETRAGFCQQFATSMAAMLRTIGIQSRVVVGYATGLPVAPGE
                     YSFSTDVAHAWVEVWFPGWGWMPFEPTPTRTNAVTYVSSGEAPCSGQDCAGETPPRGT
                     GGSATEGREAGAIEGLRPGNGPRGATGDPGTIDIAEDASPISARMALLLGVVVVGIVL
                     LLVPPARALRRRVRLRRAADEPRRLILATYEVFTERAAGIGLGRDPGETLNEYRTKVM
                     ETGYLSNGHLDRLTRLASAAAYSPREPAAEQAQEAGSAADTAIREIRRSVGPTAWFVG
                     LYRGRWT"
     gene            complement(7718..8212)
                     /locus_tag="VF195_03770"
     CDS             complement(7718..8212)
                     /locus_tag="VF195_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6579969.1"
                     /translation="MEGFTAHQACKFTGCSPRQLRYWDEIGLVQPSVQGTGGRPGVPR
                     LYSFRDLVALRVVKSLLDGGMSLQRVRRSWEFLTKKAGLTKHLSEVKLVTDGQSIFKI
                     CRRDGEVIDALRDGQMAFFVAIDDIATGVETKVRQFEEDRERFVRALRAAAATTATGQ
                     SAAR"
     gene            complement(8433..8795)
                     /locus_tag="VF195_03775"
     CDS             complement(8433..8795)
                     /locus_tag="VF195_03775"
                     /inference="COORDINATES: protein motif:HMM:NF022684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HEX6579970.1"
                     /translation="MPLNDHEQKILEEIERRLSEEDPRLVEQVTRTDLHTHAWGRMKL
                     AIAGFVVGSLMLLLVGVSVWIAAAGFVAMTLSAVLAYRYAGRLGDDTVAPGMFGRIAS
                     RVAGRLRPPPSAPPSDER"
     gene            complement(8836..10050)
                     /gene="dinB"
                     /locus_tag="VF195_03780"
     CDS             complement(8836..10050)
                     /gene="dinB"
                     /locus_tag="VF195_03780"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018500069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX6579971.1"
                     /translation="MTTPRRPAEPILHVDLDAFYASVAVLNDPSLEGKPVIVGGTGAR
                     GVVTSASYEARRFGVRSAMPSVRARRLCPEGIFLPPDFDAYRVHSHRFREVLLAHTPL
                     VEPISLDEAFLDVGGATRLFGSPVEIAKRIRREVAREVGVTCSVGIAPTKFVAKLASD
                     GCKPDGFLHVPADGVAAYLEPLPVGRLWGVGEKTAELLGRLAIRTVGDLGRTPQTILE
                     RLIGAAAASHLGALARGVDDRRVEPYEQPKSIGHEETFDRDLDDEDEILRELLALSGR
                     VAARLREDGYRARTVTLKVRLANFTTLTRSRTQPDPTDLGGEIHRIVSDLYRHLPGER
                     RRVRLLGVQAAGLDSAGAHQLALLRSERWDDVERTVDRIERRFGTGAAGPASLLDRSR
                     HRSVPLGRDPSL"
     gene            complement(10132..13410)
                     /locus_tag="VF195_03785"
     CDS             complement(10132..13410)
                     /locus_tag="VF195_03785"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6579972.1"
                     /translation="MFERAGFAHLDVRSAFSLKEGAFVPEHLAALAAAAEMPAVALTD
                     RDGLYGAARFVAACAREGVRPILGASLTVRERGRDAPLVLLAQDDRGYANLCRLITDA
                     HMLGERGDPAIVTTQICAHAAGLVAIAGPRSHAGRLAVRGRTDAAAEALHPFREAFGR
                     DRLYVAAEHRMERDSNAEIRSMLRLADRTEIRAVATNPVRYLVPEDAFLADALECMRE
                     IVPIAANHVSRRNAEGWLKPAEAMRLLFAERPDLCDATIEIAEACRFDLGLKQVHFPD
                     FPTPAGRNADAILAERCWTGVHDRGMTEDERLRDRIHLELSMIRRMGYAAFFLTVADI
                     VADIHSMGIHVACRGSAAGSLVCYLLRISDVDALRHDLAFERFLNPMRDELPDIDVDV
                     ESARREDVYDMVLSRHGDERTGCVAMIDTYRARSAVREVGKALGLPEVEVGMVAKSFP
                     HISARNLREAMAELPELDGINLPMAHLELLFRVAERLDGFPRHIALHPCGIVLSSHDL
                     VDRVPLERSANGHRMIQADKDDVELLGYLKLDVLGVRMLSSMRHATDEISRTLGEKVD
                     LERIPLDDEATFEMIRASDTLGCFQIESPGQRELLQKLQPNRWEDLIVDISLFRPGPV
                     KSDMITPYLRRRASFESPVYVHSDLRPALRETFGVIVYHEQVMRTLAALAGWDLTYAD
                     HVRRHLDHEELIPGFREEFLRRAGERGVDAEAAERTWDAVAQFASFGFCKAHAAAFAV
                     PTYRSAWLKAHYPAHFLAGILTHDPGMYPRRALLDDARRHAIPILPLDVNVSEPEYTA
                     EEVGGSEGLKERAPRASVAPQGYGIRLALQDVQGISDAEIRSILIARAERPFTNVEDF
                     LRRTSVARPVVEALAHAGAFDALPGSNRRGHLFTAITAEAAREGDQLRLSLEAPLATT
                     TGHDSTGFAFRDYTDAEVVRAELEVLGLDATRHIVSFYEPLLADLGVTSSEDVWRCRA
                     NEWVMVAGVKVSSQTPAIRSGQRIIFVTLDDGTGPLEVTVFESVQPKVAKTVFHAFAM
                     VVWGQVRRTGRRGVSVVAEEVWDLTALHRARTDGTLRETMAGPVRPTSPDRRLWHASP
                     GSAG"
     gene            13531..13773
                     /locus_tag="VF195_03790"
     CDS             13531..13773
                     /locus_tag="VF195_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018681481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2630 family protein"
                     /protein_id="HEX6579973.1"
                     /translation="MDDQDVIGRINEIAHEEHELWEKEGRGETSTAEQERLRELGVTL
                     DQCWDLLHQRRARRSAGLDPDDAAVRDPRTVEGYTG"
     gene            13852..14214
                     /locus_tag="VF195_03795"
     CDS             13852..14214
                     /locus_tag="VF195_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX6579974.1"
                     /translation="MKYMVMMFGDQATLMETRSLDWIKEMIEFMTQVDLDLKEAGELV
                     FDAGLADPTQAKTVVIEDGIPVPSDGPFAESKESLAGFWVIDVEDEARALEFASKIVA
                     FIEGPVEVRQVMDAPPEF"
     gene            14225..15484
                     /locus_tag="VF195_03800"
     CDS             14225..15484
                     /locus_tag="VF195_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX6579975.1"
                     /translation="MTSRTGIEDLLRELAPQVLGTLVRRHGQFDACEDAVQEALLAAG
                     LQWTGGVPDDPRAWLVTVASRRLVDGWRSESARRRREEITAAMEVTAPDQRTDRDDTL
                     TLLFLCCHPALSVPSQLALTLRAVGGLTTGEIAKAFLVPEATMAQRISRAKQRIRDAG
                     GRFDLPPEQDRAERLRVVLQVLYLVFNEGYAATAGPGVTRADLTTEAIRLTRLLHRLM
                     PTDGEVAGLLALMLLTDARRAARADEDGSLVPLAEQRRELWNAAQIAEGVSLVTGTLG
                     STPVGPYQLQAAIAAIHDEAPTAEATDWPQILALYEVLEHVSPGPVVTLNRAVAVAMV
                     HGPRAGLALLGTIEADEHMTHTHRLDAVRGHLLELAGDIDAARDAYRRAATRTASLPE
                     RRYLTLQAARLEAAAPTDASVRTVRGG"
     gene            15638..15745
                     /locus_tag="VF195_03805"
                     /pseudo
     CDS             15638..15745
                     /locus_tag="VF195_03805"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
     gene            15828..16124
                     /locus_tag="VF195_03810"
     CDS             15828..16124
                     /locus_tag="VF195_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX6579976.1"
                     /translation="MEETRACTPWLEAELERIGPEVLVLLGATAAQAILGRTFRVTRQ
                     RGTFVESPLAPLVTATVHPSSILRSRGDAERRAAFEGFARDLRTVTRALKARAS"
     gene            16184..17410
                     /gene="rocD"
                     /locus_tag="VF195_03815"
     CDS             16184..17410
                     /gene="rocD"
                     /locus_tag="VF195_03815"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019354810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine aminotransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HEX6579977.1"
                     /translation="MKTEPKLGSAELIELVDRHSAHNYHPLPVVVAEAEGAWVTDVEG
                     KRYLDMLAAYSAMNFGHRHPDLIRAAKEQLDRVTLTSRAFHHDLFGPFCEQLADLCGT
                     DMVVAMNSGAEAVETALKTARRWGYDVKGVAEGRAKIVVCDGNFHGRTITIVGFSSDP
                     SATGGFGPFTPGFVSVPFGDAAALATALEDPDVVAFLVEPIQGEAGIVIPPAGYLREV
                     RRLCTERSVLMIADEIQTGLGRTGTTFACDHEDVRPDVYLLGKALGGGILPVSAVVSR
                     ADVLGVFRPGEHGSTFGGNPIACAVGLEVLRLLRTGEYQQRSKDLGAWFLERLREEAP
                     TSVTEVRGRGLWFGIELTPAAAPARAVCERLLELGILAKDTHETTVRLAPPLMVSHGD
                     LEWALERIVNALAERA"
     gene            complement(17413..17658)
                     /locus_tag="VF195_03820"
     CDS             complement(17413..17658)
                     /locus_tag="VF195_03820"
                     /inference="COORDINATES: protein motif:HMM:NF039800.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEX6579978.1"
                     /translation="MTKRFDEEVLVERDGGMPSGFEWRGRRYPVVDVIGRWRIEGRWW
                     EDGRDREYWRVEARGGAVWDLYHDRQRDRWHVERLWD"
     gene            complement(17805..18761)
                     /locus_tag="VF195_03825"
     CDS             complement(17805..18761)
                     /locus_tag="VF195_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015311232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HEX6579979.1"
                     /translation="MSRTWSGKTVAVPGGSGFLGSEVVQRLEAAGASVFAPRSRDYDL
                     TMEDRVRAMYEDARPDIVIHLAARVGGIGANQRSPGTFFYDNLQMGALLIEHARRWGL
                     EKFVCVGTVCSYPKFATVPFREDDLWSGYPEETNAPYGIAKKALLVQLQAYRQEFGMR
                     GIYLIPVNLYGPRDNFDLESSHVIPALIRKCVEAEERGDDVIEVWGSGRPTREFLYVE
                     DAADGIVAAAERYEGAEPVNLGTGREISIRDLATLIAERTGFRGSFAWNATKPDGQPR
                     RALDVSRARDEFDWTARIPFEEGLDATISWYRESLMPSPAGS"
     gene            complement(18758..19780)
                     /gene="gmd"
                     /locus_tag="VF195_03830"
     CDS             complement(18758..19780)
                     /gene="gmd"
                     /locus_tag="VF195_03830"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017303001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEX6579980.1"
                     /translation="MASKRALITGITGQDGSYLAELLLSKDYEVHGVIRRSSSFNTGR
                     LDHLYTDPHDPSGRLFLHYGDLVDSTVVSRIVREVAPDEVYHLGAQSHVRVSYDIPEY
                     TGDVTALGTTRLLEAIRQEGSPARFYQASSSEMFGTNPDIPSNEQSRFYPASPYAAAK
                     VYGYWMAVNYREAYGMFCTNGILFNHESERRGGTFVTRKIARGLVDILAGRAEVIHLG
                     KLESQRDWGHAADYVEAMWLMMQADEPDDYVIATGEPRSVKDFLEEAFSYVGLDWEPH
                     VRQDPRYFRPVDPPILVGDASKARTKLGWKPKVRFEELVRSMVDAELAAAGLARPVPD
                     RSATPP"
     gene            19832..21310
                     /locus_tag="VF195_03835"
     CDS             19832..21310
                     /locus_tag="VF195_03835"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6579981.1"
                     /translation="MEEKTPPSSRTAADGARGLDERARRRVRRHFERLTPRGPSARDR
                     HRFYYDELYRMIRDQVEPGATVLDLGCGDGALLASLQPSSGVGVDFSLPTLREARQRH
                     PDLDFVCGDVERLPISGTFDYVIASNVVGYLIDVQELFYALVRVTHPSSRVIIAYYNF
                     AWEPLLWLAERFGLKTREPLQSWLSNDGLVNLVEMADLELVKFGYRTPLPVGPAWLTR
                     PLNNLLSAVPLVNRLGLISFVTARPRRAEVAPRIAEPTCTVVIPARNERGNIRDAIAR
                     LPRLGRHTELIFVEGHSTDGTFEEIEAVIAEHPELDVKVTRQDGTGKGDAVRKGFDLA
                     TGDVLMILDADLTVPPEDLTKFWSALVEERGEFINGTRLVYPMEDNAMRLANTAGNKV
                     FSLIFSWILGERVTDTLCGTKVLWARDYRRIAANRKSFGDFDPFGDFDLLFGAANLGL
                     KIREVPVRYRDRTYGTTNIDRWRHGLLLAKMALIGARKLRFR"
     gene            21307..21984
                     /locus_tag="VF195_03840"
     CDS             21307..21984
                     /locus_tag="VF195_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6579982.1"
                     /translation="MKPDPVGSHFDTVAPDYDRWKAKAHRYYDALKKSLREIVPPGSR
                     VVEVGCATGDILASLEPLTGVGLDISPAMIELAARKHPGLEFRVHDLMQGPLDERFDY
                     VVAADVAEHVPDLDTMMGSLAGMLTVGGVAIVVTANPRWAPVLHLAERLRLKMPEGDH
                     EWRSRADLASAAGRAGMRERSFDRSLVMPKEVPGLRALDSAPWAAGLRQRLGVIQRAV
                     FEPARPS"
     gene            21993..22601
                     /locus_tag="VF195_03845"
     CDS             21993..22601
                     /locus_tag="VF195_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEX6579983.1"
                     /translation="MSPPFRPGASIAIREVWRGRVFEARPTIVVEDDPGRTMLLLPGG
                     VRCGIPIGADGRELRLPDRPWRLEVRPRGERPILSFAWPDTPYSVLLWTAEEDRRVWY
                     VNLQDPLTRSAIGFDTVDHALDVVVELDRSSWRWKDEDELAEAVDRGLFTPAEASDFR
                     DWGERAVERILSMEPPFDRSWDTWRPDPAWGVPELPEGWDHL"
BASE COUNT         3486 a         7899 c         7850 g         3449 t
ORIGIN      
        1 atcgacctgg cgcccgaggt acacgaacgt ggaatccggc gtgcgcagca gccgctccac
       61 ctggcggggc tggaggtcga ggatcctcgc gatccgcgcg gcttcgccga tcgggtcggt
      121 cacgtaccgg gggtccgcgt agacgtcccg agcctccagg gtgatcgcga gcgggacccc
      181 cgttcggtcc acgatcgcac cgcgctccgc cggcaggtcg atcagatgca cccgttggtc
      241 caggccctgt tccgcgaatg cccggtggtc accgacctgc aggaccacca accgggtccc
      301 cacgcccgcc agtgccagca ccaccacgcc gagcagcgcg acgaggcggc cggcgggcgg
      361 ccgcctcact cccccgcctc gaccgtcggc gtggtgggac gcgagaagcc gggcacctgg
      421 aggatcacga cgtcacccgc ggccgggtgc acgagccctt ggcgaaccgc ccagcgaccg
      481 atccgggacg gcgaggacag atgggcgacg tcgttcacga gggtccgtcg ctcgtcggtc
      541 atcgttcgga tctcctcgac gagggatcgg ctccgatagg cagcgtccac gcgcatcgcg
      601 ttcacggaga cgaggccgac cacgagcgag gacacgacca ccgccgtgag cacccagaac
      661 gcggtgtggt gcccgcgacg cgcccgccga tggggatggg gaggggtggg gatcggcggg
      721 tgcgtcgcgg gcggccgtgc ggggctgacc gcacgacgtc gaggtcgttg ctgcggagcg
      781 ggcgcccacc ggtccgcctc gcgggccggg aggctcatgc cgcctcctcg acacgctcgg
      841 cggcgcggaa cttggcgctc cgcgcgcgag ggttgcggga gcgctcctcg gcggagggct
      901 gcagcggctt cttggtgagg acacggaggc gctcgtcgtc gcggaacgcg cgtttgacca
      961 tcccgtcctc cagggagtga taggaaagga cgaccacgcg gccaccggga ccgaggagcc
     1021 cgaccgcccg aggcagggag gtggcgagct cctcgagctc ccggttgacc gcgatgcgga
     1081 gcgcctggaa cgtccgacgc gcggggtggg ggccccccgg acgtcgtccc agggcgctcg
     1141 cgaccacacc ggccatttgg tcggtggtct cgatccgtgc ccgctgtcgc gcgcgaccga
     1201 tcgccgcggc gacgcgacgc gaacggtgct cctgcccgta ctcgaagagc agatcggcga
     1261 gctcgctctc cggaaggtcg ttcacgaggt ccgcggcggt gacgtcgccc tcgcccatgc
     1321 gcatgtccag tggtccgtcc acgcggtacc cgaacccgcg ggcgggctcg tcgagctgca
     1381 tcgacgacac tcccaggtcg aagaggaccc cgtgcagcgg ccccgtggct tcggcctctc
     1441 cgacctggga gaagcgtcga tgcaccgccg tgaagcggtc cccgtacggg gccaggcgct
     1501 cgcgcgcgac ccgcagtgcc tccggatcgc ggtcgaaccc gatcacacgc tcgacgccgg
     1561 cgtcgagcag cgcggccgca tggcccccgg cgcccacggt catgtcggcg acccgtcgcc
     1621 cggtccccga ccccccggga agcaagcact ccacgacctc ggccaccatg acgggttcgt
     1681 gcggtggacg cacggagacc ggctcatgtg accgctcgtc gttcgccacg tcacccgatc
     1741 tgtcccgcca agaccccgag gccgatcgac gcgagcaacg tcagcgccgt catcccgagc
     1801 aacgacaggc tctgtcgaac ctccgtcgaa agcttcgtct gcatcctcat ccctccgtcg
     1861 tccgtggtgc cctgtcgaaa ccggggtggc ctcccgcgac cggccgtgct cctcggggct
     1921 cgaggtcgcc gttctcgtag gcgtccgcat gggcatcccg gtaccgctcg aactcggctc
     1981 ggtcccagat ctcgatccgt tcgcggacgc caagcacgac gacctccttg ttgatcccga
     2041 ccgcgtcccg cagtcgctgc gggatcgtga cccgcccctg ggcgtcgagc ctgacctcgt
     2101 cggccgcgga gaagaagagt cgtgagaagg cgcgaccgtc cttgtcggag agggcgaagg
     2161 cgccgacctc ccgactgcgt cgctcccact cggccctggg gaagcagtac aggcagcggt
     2221 cttggccaac ggtgagccag aggccttcgt cgaacgccgc gcggaacctc gcgggcagtg
     2281 acaccctgcc tttcgggtcg agtgaatagc tatgcgtgcc gaggagctcg gccacgcctc
     2341 ctcccctctc ctccactgag ccccacgttt atgccactaa cactccactt cgtcaacggt
     2401 tcggggcaga tatctgggag ggaagacgga gaacgaagga tccgtcggcg gctcagcggc
     2461 gggttctcgg ggcggccgct acgaggcgag atagagctcc cagccgggct cgatccggcc
     2521 gagcgtcaga tggaacccgt ccctcggcgc gtagggctgt cgccgcaagg agaagccgga
     2581 ctcatgcggg gtccggtcct ctttgcgcgc gttgcacttg cgacacgagg cgacgacgtt
     2641 gtcccaggtg tgcaagccgc ctcgcgatcg ggggatcacg tgatccaggt tctccgcagc
     2701 ggcgccgcag tactggcacg tgccgaggtc acgagcgaac accgcccgtc gcgtcaacgg
     2761 cgcatgcggc cggtacggga cgtggacgta ccggcggagc cggaccacgg acggcgcctc
     2821 gatcgtcaac cgggccgagt ggaacaccag cccgttgctg aggatgacgt cggccttctc
     2881 cttcagcacc atgacgaccg cccgtctcgc cgccaccacc gccagcggcg actccgacgc
     2941 gttgagcacg agggcgcgtc ccatgcgcgc agtgtagcga cctcgcacga accacgaggg
     3001 atacgggcac ccgaatcaca accgtgtaat ctgtcgccga aggcgggccg gcccgcgcgc
     3061 gatgcctgat cttgcgacta ggggccgcgc ggggcgtccg ttcgtgggtc ggtccgccct
     3121 cggcacccag accgcggacc ccgaccgcct cggtgtcgcc gtcgccggct ccggcgtgtc
     3181 gggaacttgc cctcaccgga aggcgtctgc tacctcgacg caaccccggt tccgtcccgc
     3241 acgtaagatt gtgcacctgc taccgaggtc aggggtcgga tgcgatggag gtacgagaga
     3301 agatcccgga tccccgagcg ttcgctgctc ggttccgtga gatcgaggag aacgtcgaac
     3361 aggtcgtccg cggcaagcac gaggagatca ggctcgccct cgtcgccctg gtcgccgagg
     3421 gccacctgct gatcgaggat gttcccggcg tcggcaagac gatgctcgcc aagtcgatcg
     3481 cccggtcgat cgactgttcg ttccggcgga tccagttcac gccggatctg ttgcccaccg
     3541 atgtcaccgg cgtcaacgtc ttcagccagg agacggggga tttcgagttc aagcccggcg
     3601 cgatcttcgc caacatcgtg ctcggagacg agatcaaccg ggcgtctccg aagacccagt
     3661 ccgcgctcct cgagtgcatg gaggagcggc aggtcacggt ggataccgag acccataccc
     3721 tcggaacacc cttcatcgtg atcgccaccc agaacccgat cgagcacgag gggacctatc
     3781 cgctcccgga ggcacagctc gaccgattca tgatgcgcct cgcgatcggg tatcccgatg
     3841 ccgaggtcgc tgccgagatc ctggcgagcc acgcggccgg ggccccgctc gacgacatcc
     3901 gtcccgtgag cgacgcgagc ggtgtccgcg agatgatcga ggaggcgcgg aggatccatg
     3961 cggctccacc gatccgcagg tacatcgtcg atctggtgga agcgacccgg cggcactccg
     4021 acgtctatct cggcgccagt ccgcgggcct cgatcatgat cctgcgagcc gcgcgcgcgc
     4081 tcgccgcagc cgaggagcgg gactacgtga tcccggacga cgtgaagacc ctcgcaccgg
     4141 tcgccctgcc gcaccgcatc atcgtgacgg cggacgcgat catgagtggg cgctccgcgg
     4201 aggtgctcgt tcgggagatc atcgacgagg tcgacgtccc catcgcggag aacccctgat
     4261 gatcccgggc cagtgatgct cgcgggacgc ggcgtggccg tcttcgtgac gggtatcgcg
     4321 atgtggctcg cggcccggtt cctcggctct ccgggtctgg aggtcatcgc gatcggcatc
     4381 gccgccctcc cgctgatcgc ctcgttcgcg gcgaagcgtg gcgcccagcg gatctcggtt
     4441 cgccgacggc tgtcggacgt ccggatcagt ccgggctcga gggtcaccgt gacgctcgac
     4501 gtcgagaatc gcgctgcgat cggcacctcg ttcctcctgc tggaggacag gctcccgacg
     4561 cctctcggac gggcggcgcg gctcgtgatc ggtgggatcc gtgccgggaa gcgggaagag
     4621 gcgacgtaca cgttggtccc ccagtcgcga gggcgctatc tcctcggacc tctcgtgatc
     4681 gacgtgtcgg acccgttcgc gctcagcagg gtgcgcctgg agttcgacga tcgggaggaa
     4741 ttgctcgtcg cccccgagat cgaagatctc ggcgccgccc cggatcctgc ctcggggccg
     4801 tccttcggcg cgtcacgcgc ccgacagctg ttccggaccg gccaggagta ctacacgatg
     4861 cgctcctatc aggagggcga cgacctccgg aggatccact ggccctccgt cgctcgtacg
     4921 ggcgagctga tgatccgcca ggacgagtcg acccgtcggg ccagcgggct cgtgttcctc
     4981 gacacgcggg ccgccgccct cggccggacg cagactccgg ccttcgagcg cggggtctcc
     5041 gcgaccgctt cggtcggcgt cctcctggct cgccgaggct tcgtgctccg cctcggcacc
     5101 accgagctcc cggccgcagg gctgtccgag gagcggttcc tcgacgcgct cacggggatc
     5161 tcgcactcgg aggtccggtc gatcgggccg gccctggccc acctccgctc gggctcctcc
     5221 ccggacacct cgctcgtgtt cgtctcggca cctcccgtac ccgccgagct gagcgcgctg
     5281 atccgcgccg gcgcgggctt cggtcagaag ctcgcgatct tgatctatcc gaccgatccc
     5341 gtgcggttgc cgccggagcg gcaggcgcag ctcgaaggcc gggcgactca ggcccggctg
     5401 tccctcgccc gagccgggtg ggacgtcctc gtcctgcccc cgtcgatgag attgcaggag
     5461 cgatggcaca cgccggtgga acgtccgctc gcgcgcatcg tctgatcgcg ctcgccgccg
     5521 cggcactcct cgcggtcacg acggcgttgg cgttcgggcg ggtgttcctg ggggcgagct
     5581 cgaccctctc cctggtggca gccgcggtcg gcgcagccct gctggccgcc gcgttcgagc
     5641 ggaggagcct gctgctggcc acgttcgtga gcagcgccgg gctcgtcctc gcgatcgggc
     5701 tgatcgtctt cccccagacc acgtggtacg gcctgcccac cgccgagacg ctcagggcgg
     5761 ccctcgacgc cgccgcgctc gtgggcgagc aggcgaggat ccagcccgca ccggctgagc
     5821 cgatcgatcc gctcctcctg gcggcggtcg tctcgctgtg ggccgcggtg ttctccgcgc
     5881 acgccctggc cttccgcgcg gggagtccgc tcctggggct ggtgccaccc gtcgcactgg
     5941 tcgcgttcgc cgatacggtg ctcgaggagt tcgtgaagcc gctgtacgga gtggcgttcc
     6001 tggccgccgc actcctcgtc gtgttcgcgg acggtctcgc gcgcgtgcag ggatggggac
     6061 cggtgtgggc gtccgggcgg cgcgggatcg ctgccaccgc cggccgggga gcgcgccgcc
     6121 tcgcgttcac ggcgctggga gcggcgctgc tctcaccgat cctggtgccg gggttcgggt
     6181 ccaaggggct ggtcgacttc ggaacgccgg cagccgatcg cgtggcgatc gacccgttcg
     6241 tctcggtcga gtccgccctg acgcagaccg aacccgtcga ggtgctccgc gtgacgacga
     6301 accacccggg ctacatccgg ctcgtcaccg tgcccgagtt cgacggcgtg ggttggcgac
     6361 cggcagagat gtccgaaggc gtgccgctcg ttcctggcgc ggagctcctg gatccgggcc
     6421 gaggcgagaa gacgaccgcc cggatcgaga tcatgaggga tctggggcag cgctacctcc
     6481 cggctccctc cccgatcggt tcgatcgatg ggatcgccga tcggaccgtt cgctacgaca
     6541 gggacctgtg gaacgcgttc gtggatcgac cactgagtga aggcgacatc tacgaggtga
     6601 ccacgtatcg gccgcaggtg acggccgagg cgctccggaa catcccgttc ttcaacacgc
     6661 tcgaggatgg tcgctacacg gatgtccccg acgacctgga cgagaggatc cgggcgatcg
     6721 cactcgactg gacggaggac gccgacaacg tgttcgacaa ggtggtcgcg atcgaggaac
     6781 gtctgcatag ctccggcgag ttcgggtatc aggtcgacac cccgctccgg tcgggatcga
     6841 gctcgatcct cgagttcctg accgagacac gcgccggctt ctgccagcag ttcgcgacct
     6901 cgatggccgc gatgctccgc accatcggca tccagtctcg ggtcgtcgtg ggctacgcca
     6961 cgggcctgcc ggtcgcaccg ggcgagtact cgttctcgac cgacgtcgcc catgcgtggg
     7021 tggaggtgtg gttcccgggg tggggctgga tgccgttcga acccacgccg acccggacga
     7081 acgcggtgac ctacgtgtcc tccggcgagg ccccgtgttc cgggcaggac tgcgcggggg
     7141 agactcctcc ccgcggaacg ggaggaagcg ccaccgaggg gcgagaggcg ggcgcgatcg
     7201 agggtctcag gccggggaac ggcccccgcg gagccaccgg cgatcccggc acgatcgaca
     7261 tcgccgagga tgcctctccg atcagcgcga ggatggcgct cctcctcggc gtcgtcgtcg
     7321 tcgggatcgt gctgctgctc gtgccgccgg cacgggctct ccgacggcgg gtccgtctgc
     7381 ggcgcgccgc ggacgagccg cgacgtctga tcctggcgac gtacgaggtg ttcacggagc
     7441 gcgccgccgg gatcgggctc ggtcgcgacc cgggcgagac gctgaacgag taccgcacga
     7501 aggtgatgga gaccgggtac ctgtccaacg ggcacctcga tcggctgacg aggctggcgt
     7561 cggcggcggc gtactccccc cgcgagccgg ccgcggagca ggcccaggag gccgggagcg
     7621 ccgcggacac cgcgatccgc gagatccggc ggtccgtggg accgacggca tggttcgtcg
     7681 gcctctaccg cggccgctgg acctgaggac gatccgccta gcgggcggct gactgacccg
     7741 tcgccgtggt ggccgcagcg gcgcggagcg ctcgaacgaa ccgctcgcgg tcttcctcga
     7801 actgacggac cttggtctcg acgcccgttg cgatgtcgtc gatcgcgacg aagaacgcca
     7861 tctgcccatc gcggagggcg tcgatcacct caccatcccg ccggcagatc ttgaagatcg
     7921 actgtccgtc cgtcacgagc ttcacctcgg agaggtgttt cgtgaggccg gccttcttgg
     7981 tgaggaactc ccaggagcgg cgaacccgtt ggagcgacat ccctccgtcg agcagtgact
     8041 tcacgacccg aagggccacc agatcccgga aggagtacag gcgcggcacg ccggggcgcc
     8101 caccggtgcc ctgcaccgag ggctggacga gcccgatctc gtcccagtag cgcagctgac
     8161 gtggcgagca acccgtgaac ttacatgcct gatgcgcggt gaaaccctcc atcggacgcg
     8221 cgccccttcg atcacgacaa cttcgaaccc gaaccatccg gcgcgaacgt cacgggccct
     8281 cccggggaac ccgccctccg gcgacgcact cgcacccagg ggaaggaatg cacgttctac
     8341 acccatccgc gaagtcgtgg caagcgcagt ggtcagacgg tgtgatcgac gcgtcgttcg
     8401 cggcgcgttc gcgcacgcct tcggtgcgcg cgctatcgct cgtccgacgg gggtgccgag
     8461 ggcggaggtc ggagccgccc ggcgacgcgc gacgcgatcc ttccgaacat gcccggggcg
     8521 acggtgtcgt cgccgagacg gccggcgtac ctgtaggcga gcaccgcgga cagggtcatc
     8581 gcgacgaacc ccgcggccgc gatccagacg gacacgccga cgagcaggag catgagcgac
     8641 ccgacgacga accccgcgat cgccaacttc atccgacccc aggcatgcgt gtggaggtcc
     8701 gtgcgggtga cctgctcgac gagcctcgga tcctcttccg agagccgccg ttcgatctcc
     8761 tccaggatct tctgctcgtg atcgttgagc ggcatggcac ctccggccgc ggggagcgac
     8821 cccgttcccc tccgattata gagaggggtc ccgaccgagt ggtaccgaac ggtggcgcga
     8881 tcggtcgagg aggctcgccg gccccgccgc accggtcccg aagcgacgct cgatccggtc
     8941 cacggtgcgc tccacgtcgt cccagcgttc ggagcgcagc agcgccagct ggtgcgcgcc
     9001 cgccgagtcc aatcccgctg cctggacccc gagcagccgg acgcgtcgtc tctcccctgg
     9061 caggtggcga tacaggtcgg acacgatccg atggatctcc ccgccgaggt cggtgggatc
     9121 gggctgcgtg cgcgaacggg tgagcgtcgt gaagttcgcg agccggacct tcagcgtgac
     9181 cgtccgcgca cggtagccgt cctcgcggag acgagcggcc acccgccccg agagcgccag
     9241 tagctcccgc aggatctcgt cctcgtcatc gaggtcccga tcgaacgtct cctcgtgccc
     9301 gatggatttc ggctgctcgt agggctcgac ccggcggtcg tcgaccccgc gcgccagcgc
     9361 gcccaggtgg ctcgccgccg cggctccgat cagacgctcg aggatagtct ggggcgtcct
     9421 cccgagatcc ccgaccgtgc ggatcgccaa gcgccccaac agctccgccg tcttctcccc
     9481 caccccccag agtctcccga cagggagcgg ctccaggtag gcggccacgc cgtccgcggg
     9541 cacgtggagg aacccgtcgg gcttgcatcc gtcggatgcg agcttggcca cgaacttcgt
     9601 cggcgcgatg cccaccgaac acgtcacgcc gacctcccgt gcgacctccc gccggatcct
     9661 cttcgcgatc tccacgggcg agccgaacag gcgggtggcg ccgccgacgt cgaggaacgc
     9721 ctcgtcgagg gatatcggct ccacgagcgg ggtatgggcg aggaggacct cccggaagcg
     9781 gtgggaatga actcgatagg cgtcgaagtc gggtgggagg aagatgccct ccggacagag
     9841 gcggcgcgca cggaccgacg gcatcgcgct ccgcaccccg aaccgcctcg cctcgtacga
     9901 agcgctggtg accaccccgc gtgcgccggt cccccccacg atcaccggct tgccctcgag
     9961 cgagggatcg ttgaggactg cgaccgaggc gtagaacgcg tcgaggtcca catgcaggat
    10021 cggctcggcc ggcctccggg gggtggtcac agccacatca tgccgcggat cagcctgcga
    10081 gccatcagct gcagagccat caggccgcgg atccatcagc ccgcggatcc atcagccagc
    10141 agaccccggg ctcgcgtgcc acagccgccg gtccgggctg gtcggccgca ccggacccgc
    10201 catggtctcg cggagggtac cgtcggtgcg ggcccggtgc agcgccgtga gatcccagac
    10261 ctcctccgcg acgacgctca cgccccgacg accggtccgg cggacctgcc cccacaccac
    10321 catcgcgaac gcgtggaaca cggtcttggc caccttcggc tgtaccgatt cgaagaccgt
    10381 gacctcgaga ggaccggtac cgtcgtcgag cgtgacgaag atgatccgct ggccactgcg
    10441 gatcgcgggg gtctgcgagg agaccttcac gcccgccacc atcacccact cgttggcccg
    10501 gcatcgccag acgtcttcgg aggaggtgac gccgaggtcg gcgagcagcg gttcgtagaa
    10561 gctcacgatg tggcgggtgg cgtccagtcc caggacctcg agctccgcgc gcacgacctc
    10621 ggcgtcggtg tagtcccgga aggcgaagcc ggttgagtcg tggccggtcg tggtcgccaa
    10681 tggcgcttcg agcgacagcc gcagctggtc cccttcgcgc gcggcctcgg cggtgatcgc
    10741 ggtgaagaga tgtccacgac ggttgctccc cgggagcgcg tcgaacgcac cggcgtgcgc
    10801 gagcgcctcg accaccggcc tggccaccga ggtccgccgc aggaagtctt cgacgttcgt
    10861 gaacggtcgt tccgcgcgcg cgatcaggat cgatcggatc tcggcgtcgg agatcccctg
    10921 gacgtcctga agggccaacc ggatgccata cccctggggg gctacgctcg cgcggggcgc
    10981 tcgctccttg agcccctcag acccccccac ctcctctgcc gtgtactcgg gctcgctcac
    11041 gttcacgtcc agcggcagga tcgggatggc gtggcgccgt gcgtcatcca gcagcgcccg
    11101 ccggggatac atccccggat cgtgcgtgag gatccccgcg aggaagtgcg ccgggtagtg
    11161 cgccttcagc caggcgctgc ggtaggtcgg caccgcgaac gccgccgcat gtgccttgca
    11221 gaacccgaac gaggcgaact gcgccaccgc gtcccaggtc cgctcggccg cctccgcgtc
    11281 cacgccgcgc tctccggcgc gccggaggaa ctcctcgcgg aagccgggga tcagctcctc
    11341 gtgatcgagg tggcgccgaa cgtgatcggc gtaggtgaga tcccagccgg cgagcgccgc
    11401 gagggtccgc atcacctgct cgtggtagac gatcaccccg aacgtctccc gcagggccgg
    11461 gcgcagatcg gagtggacgt agacaggcga ctcgaagctc gcgcggcgcc gtaggtacgg
    11521 cgtgatcatg tcggacttca ccgggccggg ccggaacagc gagatgtcga cgatcagatc
    11581 ttcccaccgg ttgggctgga gcttctggag cagctcgcgc tggcctggag actcgatctg
    11641 gaaacacccg agcgtgtcgg aggcccggat catctcgaac gtggcctcgt cgtcgagcgg
    11701 gatccgttcg agatcgacct tctcccccag cgtccgcgag atctcgtcgg tcgcgtggcg
    11761 catcgaggac agcatccgca cgccgaggac gtcgagcttc aggtagccga gcagctcgac
    11821 gtcgtccttg tcggcctgga tcatccggtg gccgttcgcg gagcgctcca gcggcacccg
    11881 gtccacgagg tcgtgggacg acagcacgat gccgcacgga tgcagggcga tgtggcgtgg
    11941 gaagccgtcg agccgctcgg ccacgcggaa cagcagctcg aggtgggcca tcgggaggtt
    12001 gatcccgtcg agctcgggga gctccgccat cgcctcgcgg aggttgcgcg ccgagatatg
    12061 ggggaacgac ttcgccacca tcccgacctc gacctccggc aggccgagcg ccttgcccac
    12121 ctcgcggacc gccgagcgcg cccgatacgt gtcgatcatc gcgacgcagc cggtccgttc
    12181 gtcgccgtgg cgcgagagca ccatgtcgta gacgtcctcg cgacgggccg actcgacgtc
    12241 gacgtcgatg tccggcagct cgtcgcgcat cgggttcagg aagcgctcga acgcgaggtc
    12301 gtgccggagc gcgtcgacgt cggagatccg gagcaggtag cacacgagcg agccggcggc
    12361 gctcccgcgg cacgccacgt ggatccccat cgagtggatg tcggccacga tgtcggcgac
    12421 cgtgaggaag aacgccgcgt agcccatccg tcggatcatc gagagctcga ggtggatccg
    12481 gtcgcgcagg cgctcgtcct cggtcatccc ccggtcgtgc acgccggtcc agcagcgctc
    12541 ggcgaggatc gcgtcggcgt tccgtcccgc cggtgtgggg aagtccggga aatggacctg
    12601 cttgaggccg agatcgaacc ggcacgcctc ggcgatctcg atcgtcgcgt cgcagaggtc
    12661 gggacgctcg gcgaagagca ggcgcatcgc ctcggccggc ttcagccatc cttcggcgtt
    12721 cctgcgcgag acgtggttgg cggcgatcgg gacgatctcg cgcatgcact cgagcgcgtc
    12781 tgccaggaac gcgtcctcgg gcacgaggta gcggacgggg ttggtggcca ccgcgcggat
    12841 ctccgtgcgg tcggcgaggc ggagcatcga gcggatctcg gcgttcgagt cgcgctccat
    12901 ccgatgttcg gccgcgacgt agagacggtc gcggccgaac gcctcgcgga acgggtggag
    12961 ggcctccgcg gccgcatcgg tccgaccgcg gacggcgagc cgacccgcgt gggaccgtgg
    13021 gccggcgatc gcgacgagac cggcggcgtg ggcacagatc tgggtggtga cgatcgccgg
    13081 gtcgccgcgc tcgcccagca tgtgcgcgtc ggtgatcagc cggcacaggt tcgcgtagcc
    13141 gcggtcgtcc tgcgcgagca ggacgagcgg agcgtcgcgg cctcgctccc ggaccgtcag
    13201 cgaggccccg aggatcggcc ggacgccctc tcgcgcgcat gccgccacga accgggcggc
    13261 gccgtagagc ccgtcgcggt cggtgagcgc gaccgccggc atctccgccg cggccgcgag
    13321 cgcggcgagg tgctcgggca cgaacgcccc ctccttcagg gagaaggcgg accgcacgtc
    13381 gagatgggcg aacccggctc tctcgaacac ctgttcgata gtagggcgct cggaaggatc
    13441 ctgtccagag gccggttctc tcccccgtct gtgagactcg gtgcgacctg agatgagttc
    13501 gcgccagatt cgcgccagaa aggagttcct atggacgacc aggacgtgat aggccggatc
    13561 aacgagatcg cgcacgaaga gcacgagctc tgggagaagg agggccgcgg cgagacctca
    13621 acggccgagc aggagcggct ccgggaactc ggcgtgaccc tcgaccagtg ctgggacctc
    13681 ctgcatcaac gacgggcccg gcgctccgcg ggcctcgatc ccgacgacgc cgccgtccgc
    13741 gatccccgga cggtcgaggg gtacacgggc tgaggtcgat gtcgagagcc gcgcgccggt
    13801 tccgactcac gacgacagcg cggagaaccg acgagcgaag ggggcgtgcc gatgaagtac
    13861 atggtcatga tgttcgggga ccaggccacc ttgatggaga ccaggtcact ggactggatc
    13921 aaggagatga tcgagttcat gacccaggtc gacctggatc tgaaggaagc gggcgagctc
    13981 gtgttcgacg cagggctcgc ggatccgacc caggcgaaga ccgtcgtcat cgaggacggg
    14041 atccccgtcc ccagcgacgg cccgttcgcc gagtcgaagg agtccctcgc cggcttctgg
    14101 gtgatcgatg tcgaggacga ggcccgggcg ctcgagttcg cctcgaagat cgtcgcgttc
    14161 atcgaggggc ccgtcgaggt ccgccaggtc atggacgcgc caccggagtt ctgacgaccg
    14221 gcccgtgacc tcgcggacag ggatcgagga tctgctgcgg gaactcgcgc cgcaggtcct
    14281 cgggacattg gtgcgccggc atggccagtt cgacgcatgc gaggacgccg tccaggaggc
    14341 gttgctcgcc gcggggctgc agtggaccgg cggtgttccc gacgatcctc gagcgtggct
    14401 cgtcacggtc gcctcccgtc gtctcgtcga tggatggcgg agcgagagcg cgcgacggcg
    14461 ccgggaggag atcaccgccg cgatggaggt cactgctccc gatcagcgga ccgaccgtga
    14521 cgacacgctc acactcctgt tcctctgctg ccatcccgcg ctgtcggtgc cgtcgcagct
    14581 cgcgctcacg ctgcgggccg tgggtggcct cacgaccgga gagatcgcca aggcgttcct
    14641 cgtgcccgag gccacgatgg cgcagcggat cagccgcgcg aaacaacgga taagggacgc
    14701 gggagggcgg ttcgaccttc cgcccgagca ggatcgcgcg gagcgactgc gggtcgtcct
    14761 gcaggtcctc tacctcgtct tcaacgaggg gtacgcggcg accgcgggcc ccggtgtcac
    14821 cagagcggac ctcacgacgg aggcgatccg cctcacacgg ttgctccatc ggctgatgcc
    14881 gaccgatggc gaggtcgcag gcctccttgc cctgatgctg ctgaccgatg cacgccgcgc
    14941 cgctcgcgcc gatgaggacg gctccctcgt gccgctcgcg gagcagcgcc gcgagctctg
    15001 gaacgccgcc cagatcgcgg agggcgtgtc cctcgtgacg ggaacgctcg ggtcgactcc
    15061 cgtcggcccg tatcagctcc aggcggcgat cgccgcgatc cacgacgaag ccccgacggc
    15121 ggaggcgacc gactggccac agatcctggc gctctacgag gtcttggaac acgtgtcgcc
    15181 cgggcccgtc gtcacgttga accgcgccgt agcggtcgcg atggtccacg gtccgcgagc
    15241 gggcctggcc ctcctcggga ccatcgaggc cgacgagcac atgacccata cgcatcggct
    15301 cgatgccgtc cgaggtcatc tcctcgagct cgcgggagac atcgatgccg ctcgcgacgc
    15361 gtaccgacgg gccgcgacgc ggaccgcaag ccttcccgaa cgccgctacc tcacgctcca
    15421 agccgcccgc ctcgaggcag ccgcgccgac cgacgcgagc gtaaggacgg tccgaggggg
    15481 gtagggagtc ggcatgacct ccgcggcgga cttccttccc gatcgcacga ccctctccgc
    15541 gctccgggaa gccgcggcgg agtgccgagg gtgcgatctg taccggggcg ccactcagac
    15601 cgtgttcggc gagggacctc gcggggcgcg ggtcgtgatg gtcggcgagc aaccgggcga
    15661 caaggaggac ctcgttggac gacccttcgt gggcccggcg ggcggcctcc tcgaccgcgc
    15721 catcgaagag gcaggtctct cccgtgacga gatctacctc acgaacgtcg tgaagcactt
    15781 caagcacgtc gtgcgaggca aagcgcagga tccaccgcaa gccggacatg gaggagaccc
    15841 gcgcgtgcac gccgtggctc gaggcggagc tcgagcggat cggtcccgag gttctcgtgc
    15901 tcctcggggc gactgcggcc caagcgatcc tcgggcggac cttccgggtg acccgacaac
    15961 gagggacgtt cgtcgaatcg ccgctcgcgc cgctggtgac cgccacggtg catccatcct
    16021 cgatcctccg ttcgcggggc gacgccgaac ggcgagcggc cttcgagggc ttcgcccgcg
    16081 atctccgcac ggtcacccgg gcgttgaagg cccgggcgag ctgagcccgt atcatcgtcg
    16141 ccggcgcgca acgccgtccc gttcgaacgc cggaggatcg aacatgaaga cggaaccgaa
    16201 gctcggatcg gcggagctga tcgagctcgt cgaccgccac agcgcgcaca actatcaccc
    16261 gctccccgtg gtcgtcgcgg aggccgaggg agcctgggtc accgacgtcg agggcaagcg
    16321 gtacctcgac atgctggccg cctactccgc gatgaacttc ggccaccgcc atcccgacct
    16381 gatccgggcg gcgaaggagc agctcgaccg ggtcaccctt acctctcgag cgttccacca
    16441 cgacctgttc ggcccgttct gcgagcagct ggccgatctc tgcggcaccg acatggtggt
    16501 ggcgatgaac tccggagccg aggccgtcga gaccgcgctg aagacggccc gtcggtgggg
    16561 ttacgacgtg aagggcgtcg ccgagggccg cgcgaagatc gtcgtgtgcg acggaaactt
    16621 ccacggacgc acgatcacga tcgtgggatt ctcctccgat ccgtctgcga cgggagggtt
    16681 cggcccgttc acgcctgggt tcgtgtccgt ccccttcggc gacgccgccg cgctcgcgac
    16741 ggccctcgag gatcccgacg tcgtggcgtt cctcgtggag cccatccagg gagaggcggg
    16801 catcgtgatc ccgccggccg gctacctccg cgaagtgcga cggctctgca cggagcggag
    16861 cgtgctgatg atcgccgacg agatccagac cggtctcggg cgcaccggca cgacgttcgc
    16921 ctgcgaccac gaggacgtgc gacccgacgt ctacctcctc ggcaaggcgc tcggcggcgg
    16981 gatcctcccg gtctcggccg tggtctcgcg cgccgacgtc ctcggcgtct tccggccggg
    17041 cgagcacggg agcacgttcg gcgggaatcc catcgcctgc gcggtggggc tcgaggtgct
    17101 ccgcttgctg cggaccggcg agtaccagca gcgctcgaag gacctcgggg cgtggttcct
    17161 cgagcggctg cgcgaggagg cgcccacgtc cgtcaccgag gtgcgcgggc gcggcctgtg
    17221 gttcgggatc gagctcacgc cggcggccgc acccgcacgg gccgtctgcg aacggctcct
    17281 cgagctcggg atcctcgcga aggacacgca cgagacgacg gtccggctcg cgcctccgct
    17341 gatggtgtcc cacggtgatc tggaatgggc gttggagcgg atcgtgaacg cgctggccga
    17401 gcgggcctga tctcaatccc agagccgctc cacatgccag cgatcgcgct ggcggtcgtg
    17461 gtacagatcc cagacggccc cgccgcgcgc ctcgacgcgc cagtactccc gatcccggcc
    17521 gtcctcccac caacggccct cgatccgcca ccgcccgatc acgtcgacca ccgggtatcg
    17581 ccggccgcgc cactcgaacc cggagggcat ccccccgtcg cgctcgacca gcacctcctc
    17641 atcgaaccgc ttcgtcatcc acgcctcaca gcccgcggta cctgggttcg tccatcggtc
    17701 ggacccatgc tctggtcgac ccatggtcga acgtgtgttc gagtgggcaa ctttatgcgt
    17761 cggcgccgac aggagtcaag gacggagctc gcgagacgag acggtcagct gccggcgggc
    17821 gatggcatca gcgactcccg gtaccacgag atcgtggcat ccaatccttc ttcgaacggg
    17881 atacgggccg tccagtcgaa ctcgtcacgg gcccgcgaca catccagggc gcgtcgtggc
    17941 tgaccgtcgg gtttcgtcgc gttccacgcg aacgaccctc ggaacccggt gcgctccgcg
    18001 atgagggtcg cgaggtcccg gatcgagatc tctctccccg ttccgaggtt cacgggctcg
    18061 gcgccctcgt agcgctccgc agcggcgacg atcccatcgg ccgcgtcctc gacgtagagg
    18121 aactcacggg tgggtcgacc cgacccccag acctcgatca cgtcgtcgcc ccgctcctcc
    18181 gcctccacac acttccggat cagcgcgggg atgacatgtg agctttccag gtcgaagttg
    18241 tcccgtggcc cgtacaggtt caccgggatc agatagatgc cccgcatccc gaactcctgg
    18301 cggtacgcct ggagctgcac gagcagcgcc ttcttggcga tgccgtaggg cgcgttcgtc
    18361 tcctcaggat acccggacca cagatcgtcc tcccggaacg gcaccgtcgc gaacttcggg
    18421 taagagcaca ccgtcccgac gcacacgaac ttctcgagcc cccatcgccg tgcgtgctcg
    18481 atcagcagcg cgcccatctg caggttgtcg tagaagaagg ttcccggtga ccgttggttc
    18541 gctccgatcc ctccgacgcg ggcggcgagg tggatcacga tgtccggtcg agcgtcctcg
    18601 tacatcgccc gaacccgatc ctccatcgtg agatcgtagt cacggctgcg tggcgcgaag
    18661 acggatgcgc cggcagcctc gagacgctgc acgacctcgg agccgaggaa gcccgacccg
    18721 cccggcaccg ccaccgtctt ccccgaccag gttcggctca tggaggcgtc gcgctccggt
    18781 cgggaacggg acgtgcgagg cctgccgcgg cgagctccgc atcgaccatc gagcggacca
    18841 gctcctcgaa cctcaccttt ggcttccatc cgagcttcgt ccgcgccttg gacgcgtcgc
    18901 cgacgaggat cggcggatcg accggacgga agtaccgcgg atcctgacga acgtgaggtt
    18961 cccagtccag accgacgtag ctgaaggctt cctcgaggaa gtccttcacg gaacggggct
    19021 cgccggtcgc gatcacatag tcgtccggct cgtccgcctg catcatcagc cacatcgcct
    19081 cgacgtagtc ggccgcgtga ccccagtccc gctggctctc gagcttcccg agatggatca
    19141 cctccgctcg cccggccagg atgtcgacca ggcccctcgc gatcttccgg gtgacgaagg
    19201 tcccaccgcg ccgctcgctc tcgtggttga acaggatgcc gttcgtgcag aacatgccgt
    19261 acgcctcgcg atagttcacg gccatccagt acccgtagac cttggccgcg gcgtacgggg
    19321 aggccgggta gaagcggctc tgctcgttgg aggggatgtc cggattcgtg ccgaacatct
    19381 ccgaggagga cgcctggtag aaccgggcgg gactgccttc ctgacgtatc gcttccagca
    19441 gccgcgtcgt cccgagcgct gtgacgtccc ccgtgtactc ggggatgtca taggagaccc
    19501 ggacgtgcga ttgcgccccg aggtgataga cctcgtccgg agcgacctca cgcacgatcc
    19561 gggacacgac ggtggagtcc accagatcgc cgtagtggag gaacagtcga ccggagggat
    19621 cgtgcggatc ggtatagaga tgatccagac gcccggtgtt gaacgaactg gacctccgga
    19681 tgaccccgtg cacctcgtaa tccttggaga gcaacagctc ggcgaggtac gagccatcct
    19741 gcccggtgat acccgtgatg agcgcgcgct tggaggccat gggatgtccg cgcggtggcc
    19801 gcggagcgat gagcctatca ttcccccctt cgtggaagag aagacaccac cctcgtcacg
    19861 gacggcggcg gacggcgcgc gcggtctcga cgagcgcgcg cgccgccggg tccgccgcca
    19921 tttcgaacgc ctgacgccgc ggggcccgtc cgcacgggat cgacaccggt tctattacga
    19981 cgagctctat cggatgatcc gcgaccaggt ggagcccggg gcgaccgtgc tcgacctcgg
    20041 atgtggggac ggagcgttgc tcgcctccct gcaaccatcc tctggggtcg gtgtcgactt
    20101 ctcacttccc acgcttcgag aagcgaggca gcggcacccg gatctggact tcgtctgcgg
    20161 agatgtggag cgcttgccga tctccgggac cttcgactac gtgatcgcga gcaacgtggt
    20221 cgggtatctg atcgacgtcc aggagctctt ctacgcgctg gtgcgcgtga cgcatccctc
    20281 gtctcgcgtg atcatcgcgt actacaactt cgcctgggaa ccgctgttgt ggctcgcgga
    20341 gcggttcgga ctgaagacac gcgagccgct ccagagctgg ctgtccaacg acgggctcgt
    20401 gaacctcgtc gagatggcgg acctcgagct cgtcaagttc ggctatcgga cgcccctgcc
    20461 cgtggggccg gcgtggctga cgcgtccact gaacaacctg ctctccgcgg tccccctcgt
    20521 caatcgtctc gggttgatct cgttcgtcac ggcgagaccc aggagggcgg aggtggcgcc
    20581 gaggatcgcc gagccgacat gcaccgtcgt catcccggca cggaacgagc gcgggaacat
    20641 ccgggatgcc atcgcgcgtc tcccccgcct ggggcgtcac accgagctga tcttcgtgga
    20701 gggacactcc accgacggga ccttcgagga gatcgaagcg gtgatcgccg agcacccgga
    20761 gctcgacgtc aaggtgacgc gccaggacgg gacggggaag ggcgacgccg tccggaaggg
    20821 gttcgacctc gcgaccgggg acgtgttgat gatcctcgac gcagatctca cggtgccccc
    20881 ggaagatctc acgaagttct ggtccgccct ggtggaggaa cgaggcgagt tcatcaacgg
    20941 gacgagactc gtctacccga tggaagacaa cgcgatgcgc ctcgccaaca cagccgggaa
    21001 caaggtcttc agcctgatct tcagttggat cctcggggaa cgggtgacgg ataccctctg
    21061 tggaacgaag gtcctgtggg cccgcgatta ccgccggatc gccgctaacc gcaagtcgtt
    21121 cggtgacttc gatccgttcg gcgacttcga tctcctcttc ggtgcggcga acctcggctt
    21181 gaagatcagg gaggtgccgg tgcggtaccg cgatcggaca tacgggacca ccaacatcga
    21241 tcggtggcgg cacgggctgc tgctcgcgaa gatggccctg atcggcgccc ggaagctgcg
    21301 attccgatga agccggatcc cgtcggcagc cacttcgaca ccgtcgcgcc cgactacgat
    21361 cgctggaagg ccaaggccca tcggtactac gacgccctga agaagagcct ccgcgagatc
    21421 gtgccgcccg ggtctcgtgt cgtggaggtg ggatgcgcga ccggcgacat cctggcttcg
    21481 ctcgaaccgc tgacgggcgt gggtctcgac atcagcccgg cgatgatcga gctggcggct
    21541 cggaagcatc cagggcttga gttccgggtg cacgacctga tgcaggggcc gctcgacgag
    21601 cggttcgact acgtcgtggc cgccgacgtt gcggagcacg tgccggacct cgacacgatg
    21661 atgggatccc tggcgggcat gctcaccgtc gggggggtcg cgatcgtggt gacggcgaac
    21721 ccgcggtggg ctcctgtcct gcacctggcc gagcgccttc gattgaagat gcccgagggt
    21781 gaccacgaat ggcgctccag ggcggacctg gcctcggccg ccggccgggc cgggatgcgc
    21841 gagcgctcgt tcgatcggtc gttggtgatg ccgaaggagg tgcccggact ccgagcgctc
    21901 gactccgcac cgtgggcggc ggggctccgc cagcgcctgg gcgtgatcca gcgagccgtg
    21961 ttcgagcccg cccggccgtc ctgatcgtcc ggatgtcgcc accgttccgg cccggagcga
    22021 gcatcgcgat ccgggaggtg tggcgcggtc gagtgttcga ggcgcgtccc acgatcgtcg
    22081 tcgaggacga ccccgggcga acgatgctcc tgctgccggg tggcgtccgg tgcggcatcc
    22141 cgatcggagc ggacgggcgc gaactgaggc tccccgaccg gccgtggcgt ctggaggtga
    22201 ggcctcgcgg cgagcggccg atcctgtcct tcgcgtggcc ggacacgccc tactcggtgc
    22261 tcctgtggac ggcggaggaa gatcgacggg tctggtacgt gaacctgcag gacccgctca
    22321 cgaggtcggc gatcgggttc gacacggtcg accacgcgct ggacgtcgtc gtcgagctgg
    22381 atcggtcttc ctggcgctgg aaggacgagg acgagctcgc cgaagcggtc gaccgggggc
    22441 tgttcacgcc cgcggaggcg tcggacttcc gtgattgggg cgagcgggcg gtcgagcgca
    22501 tcctctcgat ggaaccaccg ttcgaccgga gctgggacac gtggcgcccg gaccccgcgt
    22561 ggggcgtacc ggagcttccc gagggctggg atcatctgtg acacgcgtca ggaccgccgc
    22621 gcgctcaccc cgcggcactc ggacccaccg ccgccgcggg gagctggtag atcagccaca
    22681 cgcg
//
LOCUS       DASUYD010000068        14116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1576940, whole genome shotgun sequence.
ACCESSION   DASUYD010000068 DASUYD010000000
VERSION     DASUYD010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1576940"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VF195_03850"
     CDS             <1..295
                     /locus_tag="VF195_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009109528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579984.1"
                     /translation="MGENDFAEVPTAFYGADLLLAAIAYFILQTAIIAEEGPDSRLRA
                     AIGRDLKGKVSPLLYLAAIPLAFVSPWIALAFYVFVALIWLVPDRRIESAVSE"
     gene            353..1714
                     /locus_tag="VF195_03855"
     CDS             353..1714
                     /locus_tag="VF195_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEX6579985.1"
                     /translation="MSEVNPSSILMPDRPLSLVSEYMDRGGGRALSLARERGSDWVAE
                     EVRVSGLRGRGGAGFPTGLKWRTVREDPCPTKYVVCNAAEGEPGAFKDRWLLRHDPYS
                     VLEGLAVAALAVGAEGAWICIKAGAAREVERVRQAMQEMAAADLIGDVPVELVTGPDE
                     YLFGEEKALLEVIEGNPPLPRTVPPWMEGLFRRPDSPNPTVVNNAETLANVPHILQEG
                     AEWFRSIGTEDSGTMLLTLCGDVRYPGVYELPMGFSLRELIHGIGGGAPGGRTVKAVF
                     PGASSTIVSPEQFDTPMSFDAMKAVGSALGSGAFVVYDDSACIVRATLAFSRFLYVES
                     CAQCPACKHGTGQITELLERIDRGEGSEVDVDTILARSLTVTDAQRCALPTGETLIAQ
                     SAIQVFGAEFVEHYGRPCPRPREIPVPKIVDVDEHGAFVYDDLYRLKQPDWTYSDEED
                     RTT"
     gene            complement(1730..2887)
                     /locus_tag="VF195_03860"
     CDS             complement(1730..2887)
                     /locus_tag="VF195_03860"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEX6579986.1"
                     /translation="MTEHEERPRISGSDDPAEVSSPHAIEARARVSRIVAGSVALLVF
                     ASVPAVGLSRFASADEDLFKETKHELQQSKHELHKARDKYRAQTRKIRVLQRVMNRLA
                     TRISRAEGEILQLESRLDKLQRQMMKLQIRAALLQAKLDERSREAYMSSGVPILYVLT
                     ATSAADAAARMSFLTELNRRDAVLAGKIQETTDRVARIESEVVRGRQIVELRKRSLEM
                     DRKELQRKMAQFRRLVARLRGRIEQIQTEISLLRPLAVCPVDGPHAISDNFGAPRPEP
                     GGGFHWHQGDDIMAAKGTPIVAPFDGVASVSHSFLGGLGVYVHGEYGYVYNAHLSLLG
                     TLGPVEAGDTIGYVGSTGRSSGPHDHFEWHPENGDAVDPYEFLLLVCGVPL"
     gene            4028..4303
                     /locus_tag="VF195_03865"
     CDS             4028..4303
                     /locus_tag="VF195_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579987.1"
                     /translation="MQGLPVFLDANTPITLGAGTDQDLIIVARFSDDLLFEDPVPTVA
                     VDEGVLSANLQVRILYLGYVAFTSLDTPRRTALSRGLVSRPQLSELS"
     gene            5529..5924
                     /locus_tag="VF195_03870"
     CDS             5529..5924
                     /locus_tag="VF195_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018353123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6579988.1"
                     /translation="MGTYTVRATQHARRWRCMPHTDLWTYPASLQGAKEALVGCDVEA
                     RDGSIGKVDGATHETGNAHIVVDTGPWIFGRKVVIPAGAVERIDVDDKNIFVALTKDQ
                     IKESPEYDPDRFDEGYRTRLGTYYDPYLH"
     gene            complement(7098..7247)
                     /locus_tag="VF195_03875"
     CDS             complement(7098..7247)
                     /locus_tag="VF195_03875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579989.1"
                     /translation="MNILKEHRVAPADGEGRKDLRGEAQASLALILITLAVVVSVSFV
                     GFAVS"
     gene            complement(7407..7715)
                     /locus_tag="VF195_03880"
     CDS             complement(7407..7715)
                     /locus_tag="VF195_03880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579990.1"
                     /translation="MAKNEAASRESNEEIEEEHQLDPRGTRFQIACECALKTCGRAIT
                     ITVDEYRHVRMDPRQFAVVPEHFIGNIEWIVHENDRFAAVAKREGVPADVARDENPRG
                     "
     gene            complement(8495..8818)
                     /locus_tag="VF195_03885"
     CDS             complement(8495..8818)
                     /locus_tag="VF195_03885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6579991.1"
                     /translation="MGLQPPFRARSQEKDGVVTIALSGELDMATAPILSDNLAPFEGN
                     GVSTIILDLHDLTFIDSTGLLAFLEARRRARSNGHRLLLSGTGPAARRLFELTGTQFL
                     LDERE"
     gene            8991..9374
                     /locus_tag="VF195_03890"
     CDS             8991..9374
                     /locus_tag="VF195_03890"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6579992.1"
                     /translation="MEHRLPCDVASIRSARRLATDFAQDRLSDMKLDRLALMVSEVVT
                     NAVMHGSPEADGNIGLRLEWDQDALRVVVTDGAEDFAFDPGSVEDATNGHFGLLLVDR
                     LADRWGLSMDGKTAIWLEVDTSQAI"
     gene            11136..11654
                     /locus_tag="VF195_03895"
     CDS             11136..11654
                     /locus_tag="VF195_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6579993.1"
                     /translation="MERPRSIEEQVHAQATAQPTFDDLFSDHHERLYRALYLIVGNSH
                     EAEELMQDAFVKVLERWDRIDNPAGYLYRSALNSTRSRFRRLQRVAKRTMSAGEPEDP
                     FAAADLRDQVVRSLRELPKRQRAALVLLDLLDLRSQEAAKLLRVTPATVRSLASHARA
                     ALKLSMEARDDE"
     gene            11644..12537
                     /locus_tag="VF195_03900"
     CDS             11644..12537
                     /locus_tag="VF195_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579994.1"
                     /translation="MTSSADPTLERIARRVPIPEPAFERLLRRRDRKRRNQRIAAGVV
                     GIAVFVAAVWIVTSGGAFDRNQSPAVPGGAGTGPTATEPSVPPDAEQSDWTGGGLPPE
                     GTVPSTPKEGEVVARFAKLHEGFVYVYADGRVLWFGYTSTINEQRLTPEGIDLVLSGD
                     VEPQRFLHWSDLDLPAGAWADAEIRPYVASRYAVCFYQEIGESNPGTINEGYEYPSRV
                     VGFFPEPSRAILRGKDHTYEHSGEALDPGPIDCSEVTTDEARALDTILSDVRVEDSEG
                     DEIYWETHMILPHGEWLVMYG"
     gene            12701..13630
                     /locus_tag="VF195_03905"
     CDS             12701..13630
                     /locus_tag="VF195_03905"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminaseTgpA domain-containing protein"
                     /protein_id="HEX6579995.1"
                     /translation="MAIEFASDPFISILAPLGDQKARDLFEVEAANAEYWRLFTLDRY
                     DGKTWMSSDPEGQSGHTLSTPATLPQARGYTYPANAESLNQTFRILSDFDSSHALPMA
                     QTAEEIAGPIGDITWDQYLEQAFIDGGLEQGMEYTVRSRLVVPTPEELDQVEYLAQPA
                     YGVWTELPADLDPRIGQIAERWTADATSDYRKVLAIQQHFHSEGFVYSTEVVTRTDAD
                     GLLEFLTRTRAGFCQHYAAAMAVLVRELGLPARIAVGYRSGTRQEDGSYLVKTDDAHA
                     WVEVYFDGYGWLPFEPEPISTHPNAQAGTYLNP"
     gene            complement(13929..>14116)
                     /locus_tag="VF195_03910"
     CDS             complement(13929..>14116)
                     /locus_tag="VF195_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6579996.1"
                     /translation="WAVGIGVGLIALMLTWIVGNRIAMVVWDAPVGPTVAFVGAILVG
                     IVATVVAGIQLVRSGRR"
BASE COUNT         2482 a         4549 c         4572 g         2513 t
ORIGIN      
        1 gatgggcgag aacgacttcg ccgaggtgcc gacggccttc tacggcgcgg atctgctcct
       61 cgccgcgatc gcctatttca tcctccagac ggcgatcatc gcggaggaag gtcccgactc
      121 gaggctccga gctgccatag gccgcgacct caagggcaag gtgtcgccgc tcctctatct
      181 ggcggcgatc ccactcgcat tcgtcagtcc ctggatcgcg ttggctttct acgtgttcgt
      241 cgcgttgatc tggctcgtgc ccgaccgccg catcgaatcc gccgtgagcg agtaacagcc
      301 cgggcgggga cgccgcggtc ttcccgaacg ggtcggccat atgatgccgc acgtgtccga
      361 ggtgaacccg agctcgatcc tgatgccgga ccgcccactg agcttggtga gcgagtacat
      421 ggaccgcggc ggtgggcgtg ccctctcgct cgcgcgtgag cgcggttcgg actgggtcgc
      481 cgaggaggtc cgcgtgtcgg ggctccgggg ccgaggcgga gccggattcc cgaccgggct
      541 caagtggcgg accgtgcgcg aggatccttg ccccaccaag tacgtggtct gcaacgcggc
      601 cgagggcgag ccgggcgctt tcaaggaccg gtggctcctg aggcacgacc cctactcggt
      661 tctcgagggg ctcgccgtcg cggctctcgc cgtgggggcg gagggcgcct ggatctgcat
      721 caaggccggg gctgcccggg aggtcgagcg cgtgcggcag gcgatgcagg agatggccgc
      781 cgccgacctg atcggtgacg tacccgtcga gctcgtgacc ggccccgacg agtatctgtt
      841 cggggaagag aaggcgctcc tcgaggtgat cgagggcaac ccgccgctcc cccggaccgt
      901 cccgccctgg atggaggggc tcttccgccg ccccgacagc cccaacccga ccgtcgtgaa
      961 caacgcagag acgctcgcga acgtcccgca catcctgcag gaaggcgcgg agtggttccg
     1021 atcgatcgga acggaggact cgggcacgat gttgctcacg ctctgcggcg acgtccggta
     1081 tccgggcgtg tatgagctcc cgatggggtt ctcgctgcgc gagctgatcc acggcatcgg
     1141 tggcggcgca ccggggggcc gcaccgtcaa ggccgtgttc ccgggggctt cgagcacgat
     1201 cgtatccccg gagcagttcg acaccccgat gtccttcgac gcgatgaagg cggtgggaag
     1261 cgccctaggg tccggcgcgt tcgtcgtgta cgacgactcc gcgtgcatcg ttcgggcgac
     1321 gctggcgttc tctcggttct tgtacgtcga gtcctgcgcg cagtgccccg catgcaagca
     1381 cgggaccggg cagatcaccg agctcctgga gcggatcgac cgaggggagg gctcggaggt
     1441 ggacgtggat acgatcctcg cccgatcgct caccgtcacc gatgcgcagc ggtgcgcgct
     1501 gccgacgggc gagacgctca tcgcgcagag cgcgatccag gtcttcgggg cggagttcgt
     1561 cgagcactac gggcgaccgt gtccccggcc ccgggagatc cccgtgccga agatcgtcga
     1621 cgtggacgag cacggcgcgt tcgtctacga cgatctctac cgtctgaagc agccggactg
     1681 gacctattca gacgaggaag accgaacgac ctgagggccg ctcataacgt cacaggggta
     1741 cgccgcagac gagcagcagg aactcgtagg ggtcgaccgc atctccgttc tccgggtgcc
     1801 actcgaagtg gtcgtgcggg ccggacgagc ggccggtcga cccgacatat ccaatggtgt
     1861 cgcccgcctc gaccgggccg agcgtgccga gcagggagag atgcgcgttg tacacgtagc
     1921 catattcgcc gtggacgtag accccgagcc cgcccaggaa gctgtgggag acggacgcca
     1981 cgccgtcgaa cggcgcgacg atcggcgttc cttttgcggc catgatgtcg tcgccttggt
     2041 gccagtggaa cccgccaccg ggctcgggac gcggggcgcc gaagttgtcg gagatcgcgt
     2101 gcgggccatc gacggggcac acggcgagcg gccgcaggag cgagatctcg gtctggatct
     2161 gctcgatccg cccgcgtagc cttgcgacga ggcgtcggaa ctgcgccatc ttgcgttgga
     2221 gttccttgcg gtccatctca aggcttcgct tcctgagctc gacgatctgg cggccccgca
     2281 cgacctcgct ctcgatccgc gcgacgcgat cggtcgtctc ctggatcttg ccggcgagca
     2341 cggcgtcgcg ccggttcagc tcggtgagga acgacatccg tgcggcggca tccgccgcgg
     2401 acgtcgccgt cagcacgtag aggatcggta cgccgctgga catgtaggcc tcgcgactgc
     2461 gctcgtcgag cttggcctgc agcagcgccg cgcggatctg aagcttcatc atctgccgct
     2521 ggagcttgtc gaggcggctc tcgagctgca agatctctcc ctcggcccgc gagatgcgag
     2581 tggccaaacg gttcatcacc ctctggagaa cgcggatctt ccgcgtctgt gcgcggtact
     2641 tgtccctggc cttgtgcagc tcgtgcttgc tctgctgcag ctcgtgcttg gtctccttga
     2701 acaggtcctc gtcggccgag gcgaacctgg agagaccgac cgcaggcacg gatgcaaaca
     2761 cgagaagcgc gaccgatccg gccacgatcc ggctcacgcg cgcacgtgcc tcgatggcgt
     2821 gcgggctcga cacctcggca gggtcatcgc tgccgctgat ccggggacgt tcctcgtgct
     2881 cggtcatgtc acccgaccat cggcagaccg agacgttgct tgaggaacca ccatgaaaca
     2941 gtgtcttgcg cgtcgcagag cgacggttct gcgtctgggt aacccaggca cacgaaagcg
     3001 actatgtgca ccaagcgggg cccacgtgtg gctggtgtcc gagcggttcg ggcaccgctg
     3061 ggtgcccatc acgctcgaca ccaactcctg cgggatccta ggcctgcacc cggccgcagc
     3121 cgagcaagta gatctccctg gccgattgcc ggcagagcga caaggagagg atcctcaggt
     3181 cggaggtcgt ggcgagaggg gagagtcgaa ctcccacgcc ctttcgggca ccggatccta
     3241 aggttgctgc ggccaggcac ggatcccgcc gctgcatgtg gtccctatcg tctggcgccg
     3301 ttctgcaccg cccggtgttg ttccgtcgtg agcaggaagt gaacaaatcg aggccccggt
     3361 acagcctccg cggcgcagtc ccaccaatct cttcctcctc caggaactcg gggactccag
     3421 caggacggag agcctcgcgg acagcgacct aaaggcgctt cacaccgtcg cagactggat
     3481 caagcgtttg tggtcagcct cacaaggatc tcggccgcgc tggacccgtg tgtccgttcg
     3541 tgcctgaggc cctggaacgc aagacacgtt tgggtcgctc cggagccgat cgccggccgg
     3601 agcgtgccgg atcttgtcca actttctgaa cgactacaag gaactgctcc tgcgcgctca
     3661 acccgtcgag ggcgacgacg tccaatacca agcgatcctc gttgttctca ctgacttgtc
     3721 cgcggataat gcaacagagt atgcgacgac gtccagatac agagtctcac gaaaccatgg
     3781 tatgcgcacg atggagggtg ttggatccca agttcaacga aatgaacgag ggatcagcga
     3841 tccataaccc gagtcttcat ccattcagat cacccgtgcc gttcttcctg atgcacccca
     3901 ggcgctgggc ttggatcacg gctgcgttgg acagccagaa ccgcccgctg gtcgttcccg
     3961 aagcgaactc tgcgtcgaac gcggtcggcg tgttctctca gccgacggcg gagggcgcgg
     4021 tgggacgatg cagggcctgc cggtgttcct ggacgccaac accccgatca cgctgggtgc
     4081 tggtacggac caggacctca tcattgtggc tcggttctcg gacgacttgc tcttcgagga
     4141 tccggttccg acggttgcgg tggacgaggg tgtcttgtcg gccaacctcc aggtcaggat
     4201 cctgtacctg ggctacgtcg cgttcacctc gctcgatacg ccaaggcgaa cagcattatc
     4261 tcggggactg gtctcacgac cccaactttc tgagttgagt tgaactgaat agcgtgggga
     4321 tctccataac ggcatcatgg tccccacgga cgggcacact cacccgtctc tccagccgag
     4381 atgccagagg tggaagggcc gcagtgtgtc gggcctttcc accccgatcc aggagcgttc
     4441 gatgagtgac ctacaagaac tcgaagcgct gactcatggc tgatctcgga tcctcgctcg
     4501 gccgggcttg gtggcgagac catcgggaag gcgaagatcg ttcacctcga gcgcgactcg
     4561 ctcaagtggg agtcaggctg agctcgcctc gcgtgaatcg ctcaagccct ccggcagccc
     4621 ctcgtctccg tgctcgtcca tcagatgctt cgatagttcc tcctgcgtgg agaaggtccg
     4681 tccgcacatg tgacagctgc cgatctcttc gtcgccagac tgagtctcca tctcactcat
     4741 gtggtgcctc cctctcttgc tcgacatcct tcccggttgg ccaggcgtca aacacgtgcg
     4801 ttcctcccgc cgaaatgacg cgattggccg accccttgtc cgggtacgcc tgcgtccgag
     4861 aagggaggac ccatgatcgc tgctcttgga ggtatcggga tcgttggcat catcgtgttg
     4921 gtgatcgttg tactcgtgat cctctacttc gttcggcgct agacccttgg cccagatgcc
     4981 gtccaggaag acggtgtgac ttatttcgaa cggcgtgagc aagcgtggat ccattcgtca
     5041 ctgccttctc gaccttggtg gcgaagtgga cagggagtca ctgggaaccg aggctcgagt
     5101 gcaggaggga gtcaatgacc gataccgagc gacatgattg ccagccacca tccgccgagt
     5161 cgattggcca gatctggacc tgtcccgtct gcggcgatgc ttggcgagtc gaggaaggtg
     5221 cacccatcgg cggcgacgcc tacacctggg ttcggagcgg gcgtgtgagc gaatagcgac
     5281 gcaagctgaa gcgcggggcc tcgatgcctc tctgctcagc ggccggcccc cctcaatatg
     5341 ccgacgagca ccgccccgga gcgttcgatc ctcttcgccg agcacttagc gaatgtccgg
     5401 gtcggtaacg gccagaaaga tctcagctgc gaaaccgcag gtgcgacctg catagatgga
     5461 gagcccttct gaggtgtctg cagcgtagcg aatcccaagc gtcatcgaga cgaatgaggg
     5521 tacggcgtgt gggtacatac actgtccgtg cgacgcaaca cgcaaggagg tggcgttgta
     5581 tgccgcatac cgatctctgg acatacccag caagcctcca aggagcgaag gaggcgttgg
     5641 tcggatgtga cgttgaagcg cgagacggct ccatcggaaa ggtggacggg gcgacccatg
     5701 agaccggaaa cgcccacatc gtcgttgata ccggtccgtg gatcttcggt cggaaggtcg
     5761 tcatcccggc tggggcggtc gagcggatcg atgtcgatga caagaacatc ttcgtcgccc
     5821 tgacgaagga tcagatcaag gagtcgcctg agtacgaccc ggaccggttc gatgagggtt
     5881 accggacgcg gctcgggaca tactacgatc cataccttca ttaagaagta gcccgggctg
     5941 gcatcaaacg aaacgggggc gggatcgccc ccgtttcgtc tgccttcacc ccgccggacg
     6001 ccctgctgcg acggctagct gcctgaaggg cctgaaccct cgtgctcggc tgtgccaccg
     6061 gcgtccgcct ttcccgaggc catgtgttcg agagcgtggg ccggtggccc gccgccgtgg
     6121 cctacggaga ccatcggctt gccgcagccg ctgtgagcgg ccgtttgaat ctgggcaggg
     6181 gtaccgttcg cggcgactgt gctcacatag tcgccgtgcg tccagttccc agggagggag
     6241 gagacggcgg tgagatcgca cgatccggct tggtggaacc gttcgatcgc cccgccgtgg
     6301 acgcccgcat cctgctcgtc cgacttgtcg tctccagcgc cggcgaagac gctagctgtt
     6361 gcgatgcccg acagtgcgag gacgaacgta accccgaacg caagaacgcc tcgtctcgct
     6421 cgactcacaa gacctccttc agactcgtca agcgtgggtt ggttcgggtt gcccgtcaat
     6481 cgaccgatag gccatttcgt aagccggacg atctactacg ctgccagctt cttctgtgtg
     6541 gacgggtgtc gaggctggcc gagaagcggg aagtccctct cggcgcccag aacaccgtgg
     6601 ccactccttc gggagcaaat cgccgccagg gaagtgcccc tcccgaaggg gggtacaggg
     6661 ctcccggcag gcggattgtg tgctcgccca aggcctcctg acaatcaacg aaagggctga
     6721 agcgacgcgc tccgggatgg gttcgactcg atggcggcct ccgtatcccg gatacgtcag
     6781 agagacgatg gacgacccct ccggttcctt ccccgtcccg gaggggtcgt ggtgctgaac
     6841 tttccctggg gggagaagtc agcacgcaat tggatacccg gcgaacaccg gccaaaaccc
     6901 gctcgattcg tgctttgagg tcattaggct acggggcgct ccgcctccct cagcatccgg
     6961 accagcacag cccgaagcgt ccggtcctcc tcgcagtcga tcaggagttc accaaggtcc
     7021 gggtctttgg gttcccccag tacgagccga atcgtcatgc tctctcaggt cccggttctt
     7081 ggttgggtca ggctggctca ggaaacggcg aaaccgacga acgagaccga caccacgacc
     7141 gcgagagtga tcaggatcag ggcgaggctc gcttgtgcct ccccgcggag gtcctttcgt
     7201 ccctcgccgt ccgcgggcgc gacccgatgc tctttgagga tgttcacaac caggtcttac
     7261 ccagccccgg gatccggaat cggggcgtgc ctcctccgga ggcgcgcact ccccggagcg
     7321 tccggtccgc ctcgcagtcg atcaagagct ccggcgagag tccggcatta gggctagcgg
     7381 tgcgaccgcc gctgcgggaa acctccttac cctcgcggat tctcgtccct cgcaacgtct
     7441 gccggaactc cctcacgctt cgcaaccgcg gcgaaacggt cgttctcgtg gacgatccac
     7501 tcgatgttcc cgatgaagtg ctccggcacg acagcgaact gccgaggatc catccggacg
     7561 tgccggtact catccacagt gatcgtgatt gcgcgaccgc aggtcttgag cgcgcactca
     7621 cacgcgatct ggaagcgcgt gccccgcgga tctagctgat gctcctcctc gatctcttca
     7681 ttgctctctc gtgaggcggc ttcgttcttc gccatccgat cttcccgaga actgggtgga
     7741 gtatgagcct gggcgtcctg ggcctactta gcaggtcgtt cagaggccag actcgccgtg
     7801 cggcacggcc cccgatgtgc cctatcgcgg tcggtcagcc tcccgcagca tgccaacgag
     7861 caccgcccga agcgtccggt cctcctcgca gtcgatcagg agctcggcgg tgggaggacg
     7921 gaccgatggt ggggtgtgca ggaacgccat ccggggtaag ggtcggattg agagaaggag
     7981 atgagtcatg gaaccatggg tgtgggtgat tatcgcggtt gccgctgtgc tagtgctctt
     8041 gatgctgttc cggcgcacgg ggagtagccg acggaccatt gtgacgcggc gtcgctagat
     8101 ctagcgccgc tcgaaccgtg ggtgtcgacg tcggcggaag ccggcgtcgg cccttcagtt
     8161 gatgatccga atcgtcgagc acaacaactc aacggtttgt cgtcttctca tggtcgagtt
     8221 cccggtcgtt gcggatagag ctcctcgatc tcctcgttga actccctgga cgcggcctcg
     8281 ttctcggcca tccgttcttc ccgcgaaccc gtgggcggag ttattcccac gatagccggt
     8341 cacggaagtt gcttccgagc atcaagggac gagttccctt agcgggccgc cgcggcaacc
     8401 cttccggctg gccgagcctc gccgcgtgtt ggtggggccc ctacttatcc ggtgaggttg
     8461 gctcctgggt aaacccagcg atcaagctgg ggttctactc cctctcatcg agcaggaact
     8521 gagttcccgt gagctcgaat agacgcctcg ctgcgggacc tgtcccgctc aggaggagtc
     8581 ggtgcccgtt gctccttgca cgcctcctgg cctcaaggaa ggcgagcagt ccggtcgagt
     8641 cgatgaaggt caggtcgtgg agatccagga tgatcgtcga gaccccgttg ccctcgaagg
     8701 gggccaagtt gtctgacagt atcggagcgg ttgccatgtc gagttcgccg ctcaaggcga
     8761 tggtgacgac gccatccttc tcctgagacc gggctctgaa aggcggttgc agccccatgg
     8821 cacgcccctt cctgggggat caccaatccg gtgatggccg ggagattgga gccttggctg
     8881 ccgaaccatg agaattgggg ttcggcttca tgaggtccct cgccttccag atagctagac
     8941 ctgcggggcc aggtccgttt ggctcgcgct gcatcgggca agctctacat atggagcacc
     9001 ggctgccttg tgatgttgcg tcgatcaggt cggcacgccg acttgcgacc gacttcgcac
     9061 aagatcggct gtcggacatg aagctcgatc ggctcgctct catggtgagc gaggtcgtca
     9121 ccaacgccgt gatgcacggg tcacccgagg ccgatggcaa catcgggcta cgccttgaat
     9181 gggatcagga tgctctgcgc gtcgtcgtga ctgacggcgc cgaggacttc gctttcgatc
     9241 ccgggagcgt cgaggacgcg acgaacggcc acttcggcct gctactcgtc gacaggcttg
     9301 ccgaccgctg gggactctcg atggacggca agacggccat ttggctggag gttgacacct
     9361 cccaagctat ctgacattgc cctctgccac aggcaaaacg cagattggtg gcggtggcca
     9421 gggctatcgg tgtcatccct ccaggcgcta tcggagatgc tgaagcggca gtagcgttag
     9481 cagccgcgtt agcatgcgag gcattgttcc aggtcatccg cctcggtagt aggaagggca
     9541 tctttcccgg actgaccctc tgtacgtctt ttggtatgga gtccgccgct cgggagggag
     9601 tgaagagcag gcacggccaa cgtcgacggc gacgctcgga tggaggcctc acgcgccccg
     9661 gcgcgtgtcg ctggctttgg ctggaccggc ggacgttggg caggagtgtc gctttccgcc
     9721 ggaaccgaca tcacgctgcg gcgtcgtggc taaggcagcc tcattgacct tcggcgagcg
     9781 cgaataagac cgcctcgtga agccgatcgg gcagatcgag tggaagatcg tgaccgagcc
     9841 tcggcaccac ggtcaggcga caggcaccca gttgcctttg aagcagggca tggagagcct
     9901 cattcgcccg gaggaagggg tcatcttcgc cgaccaggag ccatgtgcga acatcgctcg
     9961 caaccccttc ggcaaccggg aagtctcgaa ggtccggcgg aagagctgcc ccgatcgaga
    10021 tcactgcgtg cgctccaggt tcccttcgga aagccacctc cagcgcgagg cgcccgcccg
    10081 ccgagaatgc acccaacaga aggggtccga ggttgggtcc gagccgttcg cgcgctatct
    10141 cgatcccacg ctggaggtca tgcgacgtgt gttcggcgtc cccccagtag aaccgcgtgc
    10201 gatcggacgt atctggctga gaagatcgaa ggtaggcgac cgtcacccca gcatcgaccg
    10261 cacccatcca cagctactcc atatcccggt catccgcagt ccagccgtgc aacacgatca
    10321 gcagcgggcc aaagactccg acgggcgaat ggaggcgaac ggttggctgg atctgctggc
    10381 aggccgcggc ccatcgtcgc ttcgactcga aggacgatca gcaaactcgg cgatctcacg
    10441 agcatcgtcg agatcggagt cttgcagccg cgtcggtgcc caccacatcc cgtccgcgag
    10501 tccatctcgc aacactctga ccgcggcctt tgggtcgccc atcaccgagc gcatgcaagc
    10561 cacccagaat gtcgcctctg cccgctgctc tgggaaccga tccagcgttc ggagggccag
    10621 ccgaagtgtg cttccgtagt cacctcgttg atacgaagcg agcacttggg agcggacctc
    10681 accgaactcc gacgcgtcgt ccatccgagt cagcctatcg acatggccgt gagtgtcgct
    10741 ttgagacgcc ttgtgacgaa tccgatactc cagcgtaagt cctaggtgtt aacacttgtc
    10801 acgcgccccg gcaagcgagg gcaccttcgg tcctgtcctg gtgtacgtac taggagcctt
    10861 gagggcggat cgggaggggt ggtgacccca ggcacgacga acgtcgctga acgcatcgcc
    10921 ttaccgactt cctgaactct ctagtgggat ggttccccac gtctagcacc gggggcaccg
    10981 aggaccggtg ccgcacggcg agcctgcggt gtagatggat cttctccatc tccgcggcga
    11041 gtccggcacg tcgcaactaa cgcccgatgt actaggcggg cactattcgg ggaaagccct
    11101 ccacaaaagc ggggcctcga gcgtccatgg agcaaatgga aagaccccgg agtatcgagg
    11161 agcaggttca cgcacaggcc acggctcagc cgaccttcga tgacctgttc tccgaccacc
    11221 acgagcggct ctaccgcgcc ttgtacctga tcgtcgggaa cagccacgag gccgaggagc
    11281 tgatgcagga cgcgttcgtg aaggttctcg aacggtggga ccgcatcgac aacccggccg
    11341 gatacctgta ccggtcggcc ctcaactcga cacgcagccg gttccggcgg ctccaaagag
    11401 tagcgaagcg gaccatgagc gctggtgagc ccgaggatcc cttcgccgcc gccgacctcc
    11461 gcgaccaggt cgtccgctcc ctccgagagc tcccgaagcg ccagcgtgcc gcgctcgtgc
    11521 tcctggatct cctcgatcta cggagccagg aggcggccaa gctcctccgg gtcacgcccg
    11581 ccaccgtccg ctcgctcgcg tcgcacgctc gagcggccct gaagctctcg atggaggccc
    11641 gcgatgacga gtagtgccga cccgacgctc gaacgcatcg cccgccgcgt cccgatcccg
    11701 gagcccgcct tcgagcggct gctgcgtcga cgtgaccgca agcgcaggaa ccagcggatc
    11761 gcggcgggtg tggtcgggat cgcggtgttc gtggcggcgg tctggatcgt gacgagcggt
    11821 ggagcgttcg atcgcaatca gtcgccggcc gttccgggag gagcgggtac gggacccacg
    11881 gctaccgagc cgtccgtccc tccggatgcc gagcagtcgg actggacggg gggaggcttg
    11941 cctcccgagg gaacggttcc gagcaccccc aaggaaggag aggtcgtcgc ccgcttcgcg
    12001 aagctccatg agggcttcgt ctacgtgtac gcagatgggc gcgtgctctg gttcggatac
    12061 accagcacga tcaacgagca acgcctcacg cccgagggca tcgatctcgt tctgtccggt
    12121 gacgtggagc cgcagaggtt cctgcactgg tccgatctgg atctaccggc gggcgcgtgg
    12181 gcggacgccg agatcaggcc ttacgtggcg tcccggtacg cggtctgctt ctaccaggaa
    12241 atcggcgaat ccaaccccgg gaccatcaac gaaggatacg agtacccgtc gcgagtcgtg
    12301 ggcttcttcc cagagccgtc tcgagccatc ctccgcggca aagaccacac gtacgaacac
    12361 tcaggcgagg cgcttgaccc cggccccatc gattgctccg aagttacgac cgatgaggct
    12421 cgcgccctcg acaccatcct cagcgatgtc agggttgagg actcggaggg agacgagatc
    12481 tattgggaga cccacatgat cctcccccac ggcgagtggc tcgtgatgta cgggtgagct
    12541 ccggacccgg cgccgagagg aacccaacgt gaacgaccgg accgtcgctt ctcgcgctct
    12601 ggccgcggcg atcgccctct cggtgggggc atgtgaaggc ggcgactcgg gagccgggac
    12661 cagaccgaac gaggctgctc ccgccaacga gtcaccaacg atggctattg agttcgcatc
    12721 ggacccgttc atctcgatct tggccccact gggcgaccag aaggcgaggg atctgttcga
    12781 ggtcgaggcc gcgaacgccg agtactggcg gctcttcacc ctggaccggt acgacggaaa
    12841 gacgtggatg agcagcgatc cggagggaca gagcgggcat acattgtcca cgcctgccac
    12901 cctgccccag gcacgcgggt acacctaccc tgctaacgcc gagtccctga accagacctt
    12961 ccggatcctg agcgacttcg acagctcgca cgcactcccg atggcgcaga cggcggagga
    13021 gatcgcggga ccgatcggcg acatcacctg ggaccagtac ctggagcagg cgttcatcga
    13081 cggcggcctt gagcagggca tggagtacac ggtccgctcg aggctcgtgg tcccgacgcc
    13141 tgaggagctg gatcaggtcg agtacttggc ccaaccggcg tacggagtgt ggaccgagct
    13201 ccctgcggac ctcgatccgc ggatcgggca gatcgccgag cgatggacgg cggatgccac
    13261 atcggactat cggaaggtcc tggcgattca gcagcacttc cacagcgagg gcttcgtcta
    13321 cagcaccgaa gttgtcaccc gcaccgacgc cgacggcctg ctcgagttcc tcacgcggac
    13381 cagagctggt ttctgtcagc actacgcggc cgcgatggcc gtgcttgtcc gcgagcttgg
    13441 cctgccggcc cggatcgcgg tcgggtatag gtccgggacc cggcaggagg acggcagcta
    13501 cctagtcaag accgacgacg cccacgcttg ggtcgaggtc tacttcgacg gatacggatg
    13561 gctcccgttc gagccggagc ccatcagcac ccatccgaac gcacaggcgg gcacgtacct
    13621 gaacccctga acctgtcgcc gcggcggctg cggcgtgatc ctgcaacgag ttgacgcaag
    13681 cccgaccctt cccgtgggtg tacggatcag gagccttgtc cgcggatcga gaggggtgga
    13741 agcgtaagca cgaagaaggt cgctgggatg cgcttcgggc tgcccgactc gtccctggcg
    13801 gccccacccc cccggcgtcc ggggacgtca aacggatgag acggggcgag cctagtcccg
    13861 ggagctggag gtctcaaacg gtttcgtggg gttcgcgcca ttcgagttcc ctgcggaagg
    13921 acccggtgct aacgacggcc cgatcggacc agttggatcc ccgccaccac cgtcgccacg
    13981 attccgacca ggatcgcgcc gacaaacgct acggtcggtc cgacaggggc atcccacacg
    14041 accatcgcga tccggttccc gacgatccac gtcagcatca aggcgatcaa gccgaccccg
    14101 atcccgaccg cccagg
//
LOCUS       DASUYD010000069         4279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_254867, whole genome shotgun sequence.
ACCESSION   DASUYD010000069 DASUYD010000000
VERSION     DASUYD010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4279
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254867"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VF195_03915"
     CDS             complement(<1..132)
                     /locus_tag="VF195_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006592803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6579997.1"
                     /translation="MNDRIRSPQVRLVGADGEQIGVVATQEAVRRARELDLDLVEVAP
                     "
     gene            complement(277..627)
                     /locus_tag="VF195_03920"
     CDS             complement(277..627)
                     /locus_tag="VF195_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannose isomerase type II C-terminal
                     cupin domain"
                     /protein_id="HEX6579998.1"
                     /translation="MIVEKPWGRVVTYALNQPSSVRVITVEPGQETSVHYHRMRDETW
                     VVLDPGLSIEIGDRVVDAGPGEEFVIPAEQAHRIRCNGSGPGRILEVAYGYTTEDDTQ
                     RLQDAYGRSLEPDW"
     gene            complement(624..1127)
                     /gene="ribH"
                     /locus_tag="VF195_03925"
     CDS             complement(624..1127)
                     /gene="ribH"
                     /locus_tag="VF195_03925"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX6579999.1"
                     /translation="MTVSRGAPEGAGRRVGVVVARFHEHVTARLLEGALGGLRARGVA
                     DDAVDVAWVPGAFEIPVVAGRLASTGAYDALICLGAVVRGETAHFDLVAGEAARGIAD
                     ISRTTGIPVVFEVLATESLADAEARAGGHHGNKGWDAAETALEMADLLDRLPGGTQRE
                     QREGSTR"
     gene            complement(1124..2392)
                     /locus_tag="VF195_03930"
     CDS             complement(1124..2392)
                     /locus_tag="VF195_03930"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018503044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEX6580000.1"
                     /translation="MSALSETSVFASIPEAIDEIRRGRMVIVVDDADRENEGDLIMAA
                     ERVTPEAIAFIVRHTSGVICMPVIGERLDELEIPLMVTANTDSRRTAFTVSVDARIGV
                     TTGISAADRARTIRAIIDPETEPGDLSRPGHVFPLRYRDGGVLKRAGHTEAAVDLARL
                     AGLFPAGVLCETVNEDGTMARLPDLERFARAHDLKIISIADLIEHRRLNEVLVTKVAE
                     ANIPTRHGEFRSMAYESVTDARTHVALVMGDIDEGHDVLTRVHSECLTGDVFGSLRCD
                     CGGQLDRAMELIGREGRGVILYVRGHEGRAIGITHKLRAYALQDRGRDTVEANLELGF
                     PADQRDYGIGAQILYDLGVRSMRLLTNNPKKRAGLEGHGLAINERLPLQTDPTPQNVG
                     YLRAKREKLGHLLDLPEVDGPAIDEVEARA"
     gene            complement(2389..3018)
                     /locus_tag="VF195_03935"
     CDS             complement(2389..3018)
                     /locus_tag="VF195_03935"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX6580001.1"
                     /translation="MFTGIVEELGTVVARGGEGLVVRGSITAADAVIGDSIAIHGVCL
                     TVVELQGEQISFDLSEETLARTNLGSLGPGDEVNLERPATLASRLGGHLVQGHVDGIA
                     EVTAVRPEGEDGVRLSVRLPRELLRYVVEKGSITLDGVSLTVAALHGEMVDIALIPHT
                     LRATTLGSVGESDRVNVEVDIVAKYVARNLEYLTTDGATVVRPRKDRTA"
     gene            complement(2999..4159)
                     /gene="ribD"
                     /locus_tag="VF195_03940"
     CDS             complement(2999..4159)
                     /gene="ribD"
                     /locus_tag="VF195_03940"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014146072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEX6580002.1"
                     /translation="MRSRSAFLLPFTPAPGERRSVDRERHMRRALELATRGWGRVSPN
                     PLVGCVLVRDGRVVGEGWHEGPGSEHAEAMALRLAGDRAAGATAFVTLEPCDQHGRTP
                     PCTRALIAAGVEGVIAAGRDPNPIVDGSGFRALREAGLAVEEGLLELEAVRQNLAFAV
                     HVRSGRPFVVLKMASSLDGKAAARDGSSTWITGEKARADVQRLRAWSDAVVVGSGTAL
                     SDDPRLTVRDARYASARPPLRVIVDPSGRVTGEGRAFDEAAPTLVATTELSSPSRRQE
                     WVDAGADVTVVDRDESGVSLPALVEILGKRDIQGVLLEGGPTLAWSAIRDGVVDQIVL
                     YLAPTLIGGRDAPGWLAGAGARTLAEAPQLDLVSVERLDRDLKVVADVHRDR"
BASE COUNT          634 a         1577 c         1408 g          660 t
ORIGIN      
        1 cggtgcaacc tcgacgaggt ccaggtcgag ctcgcgtgcc ctgcggacgg cttcctgggt
       61 cgcgaccacc ccgatctgct cgccgtcggc gccgacgagg cgcacctggg gagatcggat
      121 cctgtcgttc aagcgtggct ctgtcgagac cccgcatcac tcccttcgaa aacgaagcac
      181 cggaccagcc ggtgcttgtg acggatcaga cggaacacgt gcgcagagta tagggccaat
      241 ggaacacgga tggcctctga tccctgtcga tgcagctcac cagtccggct cgagggagcg
      301 tccgtacgca tcctggaggc gctgcgtgtc gtcctcggtg gtgtaaccgt acgcgacctc
      361 caggatccgc cccgggccag agccgttgca gcggatgcga tgcgcctgct ccgcggggat
      421 cacgaactcc tcgccgggac cggcgtcgac gacccgatca ccgatctcga tcgagaggcc
      481 cgggtcgagg accacccacg tctcatcccg catccggtgg tagtggacac tggtttcctg
      541 accgggctcc accgtgatga cccggaccga cgacggctgg ttgagggcgt acgtgaccac
      601 cctcccccac ggcttctcca cgatcatcgc gtgctccctt ccctctgttc ccgttgggtc
      661 ccaccgggca accgatcgag caggtcggcc atctccagcg cggtctccgc agcgtcccac
      721 cccttgttgc cgtggtggcc gccggctcgg gcctccgcgt cggccaggga ctcggtcgcc
      781 aggacctcga agacgacggg gatcccggtc gtcctgctga tgtcggcgat cccacgcgcc
      841 gcctcgccgg ccacgagatc gaagtgtgcc gtttcgcccc ggaccacggc gccgaggcag
      901 atcagcgcgt cgtaggcgcc cgtggaggcc agccgtccgg ccacgaccgg gatctcgaag
      961 gcacccggaa cccaggcgac gtcgacggca tcgtcggcga cgccgcgcgc acggagcccg
     1021 ccgagcgcgc cctccaagag cctcgcggtt acgtgctcgt ggaacctggc gacgacgacg
     1081 ccgacgcgcc gcccggcgcc ctcgggggcg cctctcgaca ccgtcatgct cgcgcttcca
     1141 cctcgtcgat cgccggcccg tccacttcgg ggaggtccag gagatggccg agcttctccc
     1201 gcttggcccg gaggtaaccg acgttctgcg gcgtcggatc cgtctgcagc ggcaatcgct
     1261 cgttgatcgc gaggccatgc ccctcgagcc ccgcccgctt cttcggattg ttcgtgagca
     1321 gtcgcatgga tcggaccccg aggtcgtaca ggatctgggc gccgatgccg tagtcgcgtt
     1381 gatcggccgg gaacccgagc tcgaggtttg cctccaccgt gtctcggccg cgatcttgga
     1441 gggcgtaggc acggagcttg tgcgtgatgc cgatcgcgcg gccctcgtgc ccgcggacgt
     1501 agaggatcac accgcgcccc tcacgtccga tcaactccat cgcccggtcg agctgccccc
     1561 cgcagtcaca gcggagggat ccgaacacgt cgcccgtgag gcattcggag tgcacgcggg
     1621 tcaggacgtc gtggccctca tcgatgtcgc ccatcaccag ggcgacgtgc gtccgcgcgt
     1681 cggtcacgct ctcgtacgcc atcgaacgga attcgccgtg gcgcgtgggg atgttcgcct
     1741 ccgccacctt ggtcacgagc acctcgttca ggcggcggtg ctcgatcagg tcggcgatcg
     1801 agatgatctt cagatcgtgc gcgcgggcga accgctccag gtccgggagc cgcgccatcg
     1861 tgccgtcctc gttgacggtc tcgcacagga cgcctgccgg gaagagtccg gccagccgcg
     1921 cgaggtcaac ggcagcctcg gtgtgtccgg cacgcttgag gacaccgccg tcccgatagc
     1981 gaagggggaa gacgtggccc ggccgcgaca ggtcgcccgg ctcggtctcg ggatcgatga
     2041 tcgccctgat cgtgcgtgcg cgatccgccg ccgagatgcc ggtcgtgacg ccgatccgcg
     2101 cgtcgacgga cacggtgaac gcggtccgcc gggaatcggt gttcgcggtc accatcagcg
     2161 ggatctcgag ctcgtcgagt cgttcgccga tcaccggcat gcagatcacg ccgctcgtgt
     2221 ggcggacgat gaacgcgatc gcctccggcg tcacgcgctc cgcggccatg atcagatcgc
     2281 cctcgttctc ccggtcggcg tcgtcgacga cgatcaccat ccgaccccgg cggatctcgt
     2341 cgatcgcctc ggggatcgag gcgaagacgc tcgtttcgct cagcgcgctc atgccgttcg
     2401 gtcctttctc ggtcggacga cggtcgcgcc gtcggtcgtg aggtactcga ggttccgggc
     2461 cacgtacttc gcgacgatgt cgacctccac gttcacccgg tcgctctcgc caaccgagcc
     2521 cagtgtggtg gcgcgcaggg tgtgcgggat caacgcgatg tcgaccatct cgccgtggag
     2581 tgccgcgacg gtcaggctca cgccgtcgag cgtgatcgat cccttctcca cgacgtagcg
     2641 gagcagctcc cgagggagtc gcaccgagag tcggacgccg tcctcaccct cgggtcggac
     2701 ggcggtcacc tcggcgatgc catccacgtg tccctgcacg agatggcctc cgaggcggga
     2761 agcgagcgtg gcgggtcgtt ccaggttcac ctcgtccccg ggaccgagcg agccgaggtt
     2821 cgtccgcgcc aacgtctcct ccgagaggtc gaatgagatc tgctccccct ggagctcgac
     2881 gaccgtgagg cagacgccgt ggatcgcgat cgaatctccg atcaccgcgt ccgcggccgt
     2941 gatcgaaccg cgaacgacca ggccctcccc gccgcgggcg accacggttc cgagctcctc
     3001 aacgatcccg gtgaacatcg gccaccacct tcaggtcccg atcgaggcgt tccacggaca
     3061 cgaggtcgag ctgcggcgct tccgcgagcg tgcgcgcgcc cgccccggcc agccacccag
     3121 gcgcgtcgcg gccgccgatc aacgtgggcg cgaggtacag gacgatctga tccacgacgc
     3181 cgtcgcggat cgcgctccac gcgagcgtcg ggccgccctc caacaacaca ccctggatgt
     3241 cacgcttgcc gaggatctcc accagcgctg gcagtgacac accgctctcg tcgcggtcca
     3301 ccaccgtcac gtccgcgccg gcgtcgaccc attcctgacg cctcgaaggc gatgagagct
     3361 cggtcgtcgc taccagcgtc ggcgcggcct cgtcgaaggc gcgcccttca ccggtgaccc
     3421 gtccggacgg atcgacgatg acccggagcg gggggcgcgc cgacgcgtac ctggcgtctc
     3481 gcaccgtcaa ccggggatcg tcggagaggg cggtccccga gcccaccacg acggcatcgg
     3541 accacgcgcg caaccgctgg acgtccgccc tggccttctc cccggtgatc cacgtcgacg
     3601 agccgtcacg ggccgcagcc ttcccgtcga gcgacgatgc catcttgagc acgacgaacg
     3661 gacggcccga gcgcacgtga acggcgaacg cgaggttctg ccggacggcc tcgagctcga
     3721 gcagcccctc ttcgacggcg agccccgcct cgcggagcgc gcggaacccg ctcccgtcga
     3781 cgatgggatt cgggtcgcga ccggctgcga tcacaccctc gacgcccgcg gcgatcaggg
     3841 ctcgcgtaca cggcggcgtc cggccgtgtt gatcacatgg ctcgagtgtc acgaacgccg
     3901 tcgcgccggc ggctcgatcc cctgcgagcc gcaacgccat ggcttcggcg tgctccgagc
     3961 cggggccctc gtgccacccc tcgcccacca cccgcccatc gcgcacgagc acgcagccga
     4021 ccagcggatt gggtgagacg cgcccccagc ctcgcgtcgc gagctccaac gcccgacgca
     4081 tgtgccgctc ccggtccacg ctcctccgtt ctccgggagc gggagtgaac gggaggagga
     4141 aggcgctacg agaccgcacg cggcggcccc tcgcgcctgt tccctcccat cccgactctc
     4201 acggtcggtc ccgggatcgc accgggtcaa ccctcgcgtg gctcgcgagg ggtcgcggac
     4261 tgtcaccgcc ggtggggaa
//
LOCUS       DASUYD010000070         2972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1799083, whole genome shotgun sequence.
ACCESSION   DASUYD010000070 DASUYD010000000
VERSION     DASUYD010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799083"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="VF195_03945"
     CDS             <1..1285
                     /locus_tag="VF195_03945"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HEX6580003.1"
                     /translation="TETHDLWDAIEKATGEPVRRIMDGWIWQGGYPLVSARLRDGMVE
                     LGQRRYLISGKQDDTVWDVPLLVRTSAGTEPVLIEPGGVSIPAADDGPVVVNAGVHAF
                     VRVEYDDELLARLTADLRSLSTDERTQLVDDTWASVVAGHRSAVDFCRFAESFADETE
                     LPVWQSLLQGLSWCERFLTSGPREHYRSWIRGLVWPAIERIGWEPREGERDLDKPLRG
                     ALMAALGVLGADPQAFGLAMEIERESRGDGRVDPSLAAASVAIVAAGGTSEDYERYLE
                     AIAKARTPQEELRYLYALPDFRDASLVGRTLEMTLTGKIRSQNVPGVLARAMANRDHG
                     DRAWSFAKEHWDEIVSRLAPTTVVYAADGTRFLTEPDQVEDTAAFFAAHPIPQSALQL
                     QQIIERQRVNEAFRRRATPELMAAFSAGPELGEPG"
     gene            complement(1239..1466)
                     /locus_tag="VF195_03950"
     CDS             complement(1239..1466)
                     /locus_tag="VF195_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580004.1"
                     /translation="MVSDDAIRDAQRRLWNEVRVFAEPGGAAALAALLSGAAVSEPGE
                     HVVVLVCGANGDLSPIASPVRRAPDRRRTRP"
     gene            1590..2789
                     /locus_tag="VF195_03955"
     CDS             1590..2789
                     /locus_tag="VF195_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX6580005.1"
                     /translation="MAWIEKVWEGPDGARRRSGTSKKSGANEATLEADGFKVTGYLVG
                     WREPGTSTRKHEARRTRAEADRLKRQIEDQLDKHEYVPKADREIPLGTYISNLIESEE
                     SIGFLRGSTVYGYRKDLEVHIAPVLGHRPIGNITTDQLRQFFGNLKCGPSAKAKVYRL
                     LAKAFNIAVKEGRLPRSPLTAVPRPKERPVEKQLPTADDVVAIAEAADPAYRVPILVA
                     AFAGLRGGEIGGLRLQDIDFEHRKIKVRQAVRTEGGQRVIGQLKSDAARRILPVGTLV
                     DEIARHVEQFPPADDGRIFTTNHAHKGIVTHDTLNKAVRAAATRGGVEPIPNAHLLRH
                     FTASLLIRAGASIKQVQRFMGHAKAAMTLDTYADLWPEDLDELSVITDRARLEGATAP
                     VLEAAAS"
BASE COUNT          502 a          973 c         1047 g          450 t
ORIGIN      
        1 caccgagacg cacgacctgt gggacgcgat cgagaaggcg acgggtgagc cggtccgccg
       61 gatcatggac ggctggatct ggcagggcgg gtacccgttg gtctcggctc ggctccggga
      121 cgggatggtc gagctcgggc aacgccggta cctgatctcg ggcaagcagg acgacaccgt
      181 gtgggacgtg ccgctgctcg tccgcacctc ggcggggacc gaacccgtgc tgatcgagcc
      241 gggcggcgtg tccatcccgg cggccgacga cggtccggtg gtcgtcaacg ctggcgtgca
      301 cgccttcgtc cgcgtggagt acgacgacga gctactcgcc cggctgaccg cggacctccg
      361 ctcgctctcc accgacgagc gcacccagct cgtggacgac acctgggcat ccgtggtggc
      421 cggccaccga tccgccgtgg acttctgcag gttcgcggag tcgttcgccg acgagaccga
      481 gctgccggtg tggcagtcgc tgctgcaggg cctgtcctgg tgcgagcgct tcctgacctc
      541 tggaccgcgc gagcactacc ggtcgtggat ccgcggtttg gtctggccgg cgatcgaacg
      601 gatcggctgg gagccccgtg agggcgagcg cgatctcgat aagccgctcc gcggagcgct
      661 gatggcggcc ctgggcgtcc tcggggccga tccgcaggcg ttcggcctcg ccatggagat
      721 cgaacgcgag tcacgcggcg acggtcgggt cgatccgtcg ctcgccgcgg cctcggtcgc
      781 gatcgtggcg gcgggtggga catccgagga ctacgaacgc tatctcgagg cgatcgcgaa
      841 ggcgcggacg ccccaggagg agctccggta cctgtacgcg ctgccggact tccgcgacgc
      901 ctcgctggtc ggtcggaccc tggagatgac gctcaccggc aagatcaggt cgcagaacgt
      961 ccccggtgtg ctcgcgcgcg cgatggccaa ccgcgaccac ggcgatcgcg cgtggtcatt
     1021 tgcgaaggag cactgggacg agatcgtgtc ccggctcgcg cccacgacgg tcgtctacgc
     1081 ggccgacggc acgcggttcc tcaccgaacc cgaccaggtc gaggacaccg ccgcgttctt
     1141 cgcggcgcat ccgatccccc agtcggcgct gcaactgcag cagatcatcg agcgacagcg
     1201 cgtgaacgag gcgttccgcc ggcgcgcgac ccccgagctc atggccgcgt tctccgccgg
     1261 tccggagctc ggcgaaccgg gctagcgatc ggcgagagat cgccgttcgc cccgcagacg
     1321 agcacgacga cgtgctcgcc cggctcggag acggcggcgc cggagagcaa ggccgcgagg
     1381 gccgcggcgc ctcccggctc ggcgaacacg cgaacctcgt tccagagccg acgctgcgca
     1441 tcgcggatcg cgtcgtccga gaccaggacg agtccgcatc cgttctacct gcataaatgg
     1501 ggcgtttcaa gagctgggtt gacactgggt ggacaggagg tgcagactgg ggtgagtggg
     1561 cactcagccc caaggcgacg gtgggcacga tggcgtggat cgagaaggtg tgggaaggcc
     1621 ccgacggcgc tcgccgccgg agtggcacgt cgaagaagag cggggcgaac gaggcgacgc
     1681 tcgaagcgga cgggttcaag gtgaccgggt acctcgtcgg atggcgcgag ccggggacca
     1741 gtaccaggaa gcacgaggcc cgccggacga gggcggaggc ggatcgcctg aagcgacaga
     1801 tcgaggacca gctcgacaag catgagtacg tgccaaaggc ggatcgagag attccgctcg
     1861 ggacgtacat ctcgaacctg atcgagtccg aggagtcgat cggcttcctt cgcggatcga
     1921 cggtatacgg gtatcggaag gacctggagg tccacatcgc gcctgtcctc gggcatcgcc
     1981 cgatcggcaa catcacgacg gaccagctcc gtcagttctt cgggaacctg aagtgcggac
     2041 catcggcgaa ggccaaggtc taccggctcc tcgcgaaggc gttcaacatc gccgtcaagg
     2101 aggggcgtct ccctcggtca ccgctgacgg cggttccacg tcccaaggag cgcccagtcg
     2161 agaagcagct ccccacggcc gatgatgtcg tcgcgatcgc ggaggctgcg gaccccgcgt
     2221 acagggtccc cattctcgtc gcggcgttcg ccggtctccg aggtggcgag atcggcgggc
     2281 tccgtctcca ggacatcgac ttcgagcacc gcaagatcaa ggtccggcag gccgtcagga
     2341 ccgagggcgg ccagcgcgtt atcggccagc tgaagagcga tgcggctcgt cggatacttc
     2401 ctgtcgggac gctcgttgac gagatcgccc gtcacgtcga gcagttccca ccggccgacg
     2461 acggccgcat cttcacgacg aaccacgccc acaaggggat cgtgacccac gacacgttga
     2521 acaaggctgt cagggccgcc gcgacacgcg gtggagtcga gcccatcccg aacgcgcatc
     2581 tactgcggca cttcaccgcc tcgctgctca tccgggcggg ggcgtccata aagcaggtgc
     2641 agaggttcat ggggcacgcc aaagccgcga tgaccctaga cacgtacgcg gacctgtggc
     2701 ccgaggatct ggacgagctg agcgttatca cggatcgagc gaggcttgag ggggcgacgg
     2761 ctccggtgtt ggaggctgcc gcgtcctgat tccgaacgga aggatgtcag gggtcgggtt
     2821 gtcgcccggc ccctcctcgt tacccatctc cttccgccat cgcggcctgg acctcatcaa
     2881 cggcgggaag gtaacgtgca attacgttct gggcgtcgct cgtctgacgg actgaagcgc
     2941 tcgctagctc tttgaccttt ggaagatcta cg
//
LOCUS       DASUYD010000071        10851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_35923, whole genome shotgun sequence.
ACCESSION   DASUYD010000071 DASUYD010000000
VERSION     DASUYD010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10851
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35923"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            790..2775
                     /locus_tag="VF195_03960"
     CDS             790..2775
                     /locus_tag="VF195_03960"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6580006.1"
                     /translation="MTAAVAALLVFSPAGPILALGSGQQGLAADAAATVIARYRARIP
                     ELMTEQGIPGLAVALVDADEALWVEGFGYADRQGSAPVTADTIFSVQSMSKLFTATAV
                     MQAVAAGRLDLDEPITTYLPEFTVHSAFEEHPERKITLRMLLSHTAGFTHEAPVGNNN
                     ELDPGDFDAHVRSISDTWLRFPVGSGYAYSNLGIDLAGYILERVEGKPFAEVMRDSLL
                     EPLGMDHSTFERTVIRSTDDRAVGHVDPYPVLPLDVPMTAAGGLYTSATDLARFLRFQ
                     LNDGSIDGRVVLDPKSMDEMRTVLAPSAGAPAGYALGVSRTRWNRWGQRPDLFFHGGG
                     GFGFVSDLWWLPQLGIGIAILTNSEDHQLQGDLALSILADLVTAPGVYQDRLLALPER
                     PSVVDPSLSFEPRAGMAALVASAAMPPTGDETARWSTFIGAYGAPAWGVIDPTTPAAR
                     FFVDGGVPYFEADEEGSHVRHRLVEVDPEVFLADNGETLDLAGPVPTWRSIRIVRLSG
                     GPVSWQWAILVMAAVLAVIWLVAAAVRSLGRSRSRSRSRLEPSSPHRWRRIAAAVAGF
                     TALLILANVALLAWMPGLVDSGFLGSLEFPLAERLILHLPLALAGLGVIAVVLAGLAW
                     IGRWWSRAVTLQYAALAVGTIALVAQIGVWRLIGWGL"
     gene            complement(3320..3640)
                     /locus_tag="VF195_03965"
     CDS             complement(3320..3640)
                     /locus_tag="VF195_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580007.1"
                     /translation="MHWDIGLQGVGVLAAYSLGFGLIAQLVLWRSTTHWLWLIAAGTF
                     FVAGLLISEAWFGSATEGELQPNIDGLSRDEALLAFIPASIAVWITWAFGRRQMRHRP
                     GHAM"
     gene            complement(4261..4470)
                     /locus_tag="VF195_03970"
     CDS             complement(4261..4470)
                     /locus_tag="VF195_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580008.1"
                     /translation="MIACRPILPDGASRSAKTRNSSGQRARGIDSIISIDTIASYVPG
                     VREKSSRRTSTRSPSPSCAIRSLLA"
     gene            4521..4649
                     /locus_tag="VF195_03975"
     CDS             4521..4649
                     /locus_tag="VF195_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580009.1"
                     /translation="MFPGGQVPSPGVLWADAAGAGLRILAREGFGGVLRADAGGVA"
     gene            4999..5442
                     /locus_tag="VF195_03980"
     CDS             4999..5442
                     /locus_tag="VF195_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009779904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEX6580010.1"
                     /translation="MDEAAPETITQIVHGNEEFSTLLQAVGSAGLAETLSGDGPFTVF
                     APTDDAFAALPEGTLQSLLAPDQQDQLAGILTYHVVPAEVPASEVTSGDVETVNGANL
                     TVSVEDGTVTLEDGQGNEALVTQTDIEASNGVIHVIDAVLLPPSA"
     gene            5509..6447
                     /locus_tag="VF195_03985"
     CDS             5509..6447
                     /locus_tag="VF195_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEX6580011.1"
                     /translation="MDVAITGSNGLIGSALARSLEHDGYRVVRVVRPGSPAVSGDAVM
                     WDPRNGVIDRDGLDGLDAVVHLAGAGIGDRRWTPERKEEILRSRTEGTALLTSTLASV
                     PSPPSVLVSGSATGYYGDGGGDELTESSPAGRGFRAEVVAAWEAAAAPAIEAGIRTVF
                     VRTANVLTPNGGLLPYLLTPFRLGLGTRFGSGRQWFPWITLDDEVAVIRFAIESPTLR
                     GPVNAAAPEPVTNRDLVTILGRVLRRPAPWIAPALVLRLIAGAERADEALLSSAKVVP
                     AALERAGFAFGDPELEPALRRLLAPEEARTARVPVR"
     gene            6444..6878
                     /locus_tag="VF195_03990"
     CDS             6444..6878
                     /locus_tag="VF195_03990"
                     /inference="COORDINATES: protein motif:HMM:NF037099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580012.1"
                     /translation="MSAVPVEGLHELLFPGTSQTDVSSRIGRLRWFNLAMGVVHAAQA
                     VLILALATGFSIPIVIHSLTGPPGSSAETITLFDLPIAWAVAAFLLVSALAHLLIASV
                     GFRSYRRMLLVGRNDYRWIEYSITWGAGHISSPRLSQQSASG"
     gene            7073..7780
                     /locus_tag="VF195_03995"
     CDS             7073..7780
                     /locus_tag="VF195_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580013.1"
                     /translation="MHISITGLGHVAVVGVDTSGVVAQLVLAAATQGGPEELRRAFLG
                     QSERVASLCRLPHVVTCRRSEEAGPAIREIELELMRRARHFVEEGVDDVRAHLAEHSD
                     EQLPAIILLFEEPLATEIGMIDALGQQAAKLGAAVLTSGSATGAARLVVRADRSLKLE
                     TDLPLPSSLEPLALDDPTQQEAVEVIREAYPSEPAEPLEEPSEAVAAGLHSGPSSKAI
                     HTDLTGGQLGRSRRSQE"
     gene            7790..7993
                     /locus_tag="VF195_04000"
     CDS             7790..7993
                     /locus_tag="VF195_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580014.1"
                     /translation="MTPTRYSALFRLEHVPVAFIADGLARLQVRYPEIPVVFADSRKF
                     AEEWTYRFLAAALADSMGSGIAR"
     gene            8361..8519
                     /locus_tag="VF195_04005"
     CDS             8361..8519
                     /locus_tag="VF195_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580015.1"
                     /translation="MMLPDFDRADRIGSYWGNPKTRTFAELLIDCEEDRTLRAVLVGM
                     LREDERSP"
     gene            8647..9138
                     /locus_tag="VF195_04010"
     CDS             8647..9138
                     /locus_tag="VF195_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580016.1"
                     /translation="MPPGQESPSFETFFEDEKDRLLRVLCVITGSRGEAEDLAQEAFT
                     RMFERWDEVGHMDDLAGYLHRTAMNLFRSQYRRARVALKRAALSTPQRDVFEAVEERD
                     VATQALARLTPRQRAALVLTEALGYSGEETGRLLGIKASTVWALTHQARTALKGTLEA
                     TDA"
     gene            9131..10327
                     /locus_tag="VF195_04015"
     CDS             9131..10327
                     /locus_tag="VF195_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580017.1"
                     /translation="MPETRTLLERVMERVELRQFTLEGFHDRRDHKRRNQRIRAGVLG
                     IAVFALAAIGLIRLLGSEPIPADQPSSAFVGTWTSTELDDESSQTMTIRPAEDGALDI
                     TLQNDSSFLCSGRQTRLGGVDTPSTMTGTGRLEDATTLVAPSLQVCPSGWALDIGPLT
                     LEGRATSYTLVLDVATDRLFDNLGVVWHRGATPENAADTTTECEILEPQVGSPGTCSM
                     LRGEVTFRAAEPWFDHVDSYIDPRLFFLTSGPGLPDRRVTMDAMIEILANPNPLPEGP
                     CYRYPRLPASAEALVQAIRSNPDLEATMPVTERVGGIDALRLDVAPVPGASTCPNGAV
                     PVVSTSNGGWGTMGHGSLGRLYVLDLPGGSARALVIMIEAPEADFERAVEAAAPVVDS
                     FEFHTR"
     gene            complement(10495..>10851)
                     /locus_tag="VF195_04020"
     CDS             complement(10495..>10851)
                     /locus_tag="VF195_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580018.1"
                     /translation="SIYRCLKRHGLIELRRRRKRRDEFRRWERERPMQLWQMDVMGGV
                     ELGRYRALNGGRAELTESELESVGATVHVHDTSGGWHQPNTAPYELSSILSAEDTELL
                     QALMNAGSSDRVEFLG"
BASE COUNT         1683 a         3736 c         3621 g         1811 t
ORIGIN      
        1 cgcggtcgca tcgatccgcc actagaaccg ggacggttta cgaggaggca aggaggagag
       61 atcggagaga cgagaacaga ctccacgatt tctccccagt gcacccaact ggacagtact
      121 cgaagaaatg cccgcaggta gaagggcatg tgctgtctct gagcgcccgt cacgcgaagc
      181 ttcggcggga aggatacggg aagcacggag gccgatgagg gcgcgatccc ttcgcaccaa
      241 ccgcggttga gaaggggcga atcggtcggc aagacgagag ccgaggtcgt gcacgatcag
      301 gcagccaccg aacaaccccg ccctgactgt cagccctccc cctcagctcc acgatctctc
      361 ccctcccttc ttgttggcga tctcttcctt gttggcgatc ttttcgctgc atgacctccc
      421 gcagcccagg cttctcggtc gacggaccta tccgaactgc gggtcacgcc gctggagctc
      481 ttcgatctcg cctctccggc ggggggccgt gccgccttca gctgacgcga catcctggcg
      541 gagctgctgg gagtgcactt gcggcatgga tcaggggaag ctcaaccccc taagttccgg
      601 ttcgcactgt ttgtggttcc ctgctgaccc gtccagaggt cccaccaagg ggggtgcaga
      661 cggcccccgg tagcagacgt ctcgggcgag agcctgacgg tagctggcac cagccgatcg
      721 caggcggggt gcgggctgcg ggaggctgaa ttggttggct ccggtgcgcg acgcgggctt
      781 cgcggatgcg tgacggctgc cgttgctgcc cttctggtgt tctcgcccgc cggcccgatt
      841 cttgcgttgg gctcggggca acagggtctg gcggccgatg cggcggcgac ggtcatcgcg
      901 aggtaccgag cccgcatccc ggagctcatg accgagcagg ggatacctgg gcttgccgtc
      961 gccctcgtcg acgcggacga ggcgctgtgg gtcgagggct tcggctatgc ggatcgacaa
     1021 ggctccgccc cggtcaccgc cgacacgatc ttcagcgtcc agtcgatgtc gaagctgttc
     1081 acggcgaccg ccgtcatgca ggccgtcgcg gcgggccgtc tcgacctcga cgagccgatc
     1141 acgacctacc tgcccgagtt cactgtccat agcgccttcg aggagcatcc cgagcggaag
     1201 atcacgctcc ggatgctcct cagccacacc gccggcttca cccacgaggc cccggtgggg
     1261 aacaacaacg agctcgaccc cggcgacttc gacgcccacg ttcgcagcat ctccgacacc
     1321 tggctgcgct tcccggtcgg cagcgggtac gcgtactcga acctcgggat cgacctggct
     1381 ggttacatct tggagcgagt cgagggcaag cccttcgcgg aggtcatgcg ggactcgctg
     1441 ctcgagccgc tgggcatgga ccacagcacg ttcgagcgga cggtgatccg gtcgaccgac
     1501 gatcgcgcgg tgggccacgt ggatccctat cctgtcctgc cgctcgacgt gccgatgacg
     1561 gcagccggtg gcttgtatac gagcgccacc gatctggccc gtttccttcg cttccagctg
     1621 aacgacggct cgatcgacgg tcgggtcgtg ctcgatccga agtcgatgga tgagatgcgg
     1681 accgtcctgg cgcccagcgc cggcgcaccg gccggctatg cgctcggcgt gagtcgcacc
     1741 cgctggaacc gctggggcca gcggcccgat ctcttcttcc acggaggcgg gggctttgga
     1801 ttcgtgtccg acctctggtg gctgccgcag ctggggatcg ggatcgcgat cctcaccaac
     1861 tcggaggacc accagctgca gggcgacctc gccctgtcga tcctggccga cctggtgaca
     1921 gcgccgggcg tgtaccaaga tcgcctgctc gcgctcccgg agcgtccgtc cgtcgtcgat
     1981 ccgagccttt cattcgagcc gcgggcaggc atggccgctc tcgtcgccag cgccgccatg
     2041 cccccgaccg gtgacgaaac tgcccgctgg tccaccttca tcggagccta cggggcgcca
     2101 gcctggggcg tgatcgaccc caccactccc gctgcccgat tcttcgtgga cggcggcgtc
     2161 ccgtacttcg aggccgacga ggaggggtcg cacgtgcgcc accgcctcgt cgaggtcgac
     2221 cccgaggtct tcctcgccga caacggcgag acgctcgatc tcgctggtcc cgtcccgacc
     2281 tggcggagca tcaggatcgt gcgtctctcg ggtggtcccg tctcctggca gtgggcgatc
     2341 ctggtcatgg ccgcggtcct tgccgtcatc tggctcgtgg ctgccgcagt tcgaagcctt
     2401 gggcgatccc gctcgcggtc gcggtcgcgc ctggagccct cgtcgccaca ccgctggcgt
     2461 cggatcgcgg cggcagtcgc cggtttcacg gccctgctga ttctggcgaa cgtcgccttg
     2521 ctcgcctgga tgcccgggct cgttgactcc ggcttcctgg gctcgttgga gttccccctg
     2581 gccgagcggc ttatccttca cctcccgctc gcgctcgccg ggctgggcgt catcgcggtc
     2641 gtcctcgcgg gccttgcgtg gatcggtcgc tggtggtcga gggcggtgac gctccagtac
     2701 gcggcgctgg ccgtcgggac gattgcgctc gtcgcgcaaa tcggtgtctg gcgcctcatc
     2761 ggctggggcc tttagctgag ttggtcggaa cggctaacgg cacatagaac ggggagacct
     2821 tgatggtgga ggcgtcaacg tgtgccccag cgacccgccc ggaaactcgt tcctgtaccg
     2881 gatccggccg gctcatgtca gggcgcagac gcgacggagg caagcacatg accacgtcat
     2941 ccttccggct agacggagca catgcccttc tgacctagcg ccccaccatc ttgttttgcg
     3001 ctgggatacc ggaagcgcga gcacggtgca cggggagaaa tcgtggagcg aacacggagt
     3061 tgtccgctgc tcctcccaat gcggacattt gttcgagtac tgtccagttg tcctccccga
     3121 ccgctcgccg ccgagatgag tccccgcgca acctcgcctg cgctgggtac ccggctgtcg
     3181 ggtcgccctc accgcgggga tgcgggcgtt gctgggctga cgaccaggaa atacgtcctc
     3241 atgggaaggc ccgaacgccc ctgcgcttca caacccggcc accttcaccg accaccgcta
     3301 gttcccgcaa acggaacgtc tacattgcat ggccagggcg atgacgcatt tgcctccgcc
     3361 cgaacgccca agtgatccaa acggcaatgc tcgccgggat gaacgcgagc agggcttcat
     3421 cgcgtgacag gccgtcgatg ttcggctgca gttccccctc cgtcgccgaa ccgaaccacg
     3481 cctcgctgat gagcaggccg gcgacgaaga acgtcccagc cgcgatcagc cacagccagt
     3541 gcgtcgtaga cctccacagg acgagctgcg cgataaggcc gaagccgagc gagtacgccg
     3601 cgagcactcc gacgccttgg agtccgatgt cccaatgcat gcccaaacac ctcccacgct
     3661 tgtctatcgc caggcgcact cctccactag ggtcctttga cctacgagtg gcgggccggg
     3721 cgtcgtcctt cgtcgagccc ggcacggcgg cgaggtccgt cggattgacc acccccccgc
     3781 tatccccggc gcaacgtccc ggcgccccgc ccgggcttgc gccccagcgc cggacgtttc
     3841 ctactgcgat gcttcacgcg cgatctgctc gagagtcgac gcgttacggg tcggaccata
     3901 gcatggtccg cgcgaggtac cgtcacaggg aacgatccgg gcagcaagca ctagccagtg
     3961 ttcagcgtcg ggggttgacg tcgcgctttg gcggaaccgg agcggcgagt gatggggagg
     4021 gatcggggag gacctaacgc tccctgtccg ctcctcgaaa gctctccgaa aactcctcag
     4081 tgcacccggc ccgaaccgga cgaaagggac gaaagccggc gagcgccttc gtgcaggtag
     4141 aggcgacgta cagcctcgta gcttgatcgg actgtctcaa ggggaaaccc agaacaccct
     4201 gcgctgcgag gggatggtcg cgccccagtg ttcgcctcgt cgggctcggt cgaagcgagc
     4261 ttaggccagc agtgaccgga tcgcgcagga agggctcggg gaccgggtcg aggtgcgacg
     4321 ggaggacttc tcccggacgc cgggcacgta cgacgcgatc gtgtcgatcg agatgatcga
     4381 gtcgatcccg cgagctcgct ggcccgagct gttccgggtc ttcgccgacc ggctggcccc
     4441 gtcgggaagg atcggcctgc aggcgatcac cgtcggcgac cgtcactggg cctcgtcgaa
     4501 cgccgatccc gacttcgtac gtgttccccg gcggccaggt gccgtcgccc ggcgtcttgt
     4561 gggccgacgc cgcgggcgcc ggactccgca tcctcgcccg cgagggcttc gggggcgtcc
     4621 tacgcgcgga cgctggcgga gtggcgtagg ccgttcgatg ccgcgtggtc gacgatcgcc
     4681 gcgctcgggt tcgtcgagcg cttcaagcgg atgtggcagt actacctctc ctatgcgagg
     4741 gagggttccg cagcggccgc accgacgtga gccagatcgt tctcgagccg ccgaggtgac
     4801 gccccgctac ccgggccgct gaaacccatg cgtactgcat aggtttgcgc attggtttca
     4861 cgcgcctcat cgtccatgct gttcggagtc gaccagaagg agggatgaag gatgcggaag
     4921 ctcgtggtca tcaccgcggc cgtgtccgtg ttcgccctcg ccgcctgcgg cggggacgag
     4981 gagccggcga ccgaccggat ggacgaagcc gctccggaga cgatcacgca gatcgtccat
     5041 gggaacgagg aattctcgac gctcctccag gccgtcggct cggcgggact cgcggagacc
     5101 ctgtcgggcg atgggccgtt cacggtgttc gccccgaccg acgacgcgtt cgccgcgctc
     5161 cccgaaggga cgctccagtc gctgctggcg cctgaccagc aggaccagct cgcgggcatc
     5221 ctcacgtacc acgtcgtgcc ggcggaggtc cccgcctccg aggtcacgtc tggggacgtg
     5281 gagacggtca acggagccaa cctcaccgtc tcggtggagg acggcaccgt cacgctcgag
     5341 gacgggcagg gcaacgaggc cttggtcacg cagaccgaca tcgaggcctc gaacggcgtc
     5401 atccacgtga tcgacgccgt cctgctgccg ccctcggcat agcagcggaa ccccggccgg
     5461 cgggttcccc accgccggcc gggaccgcct caacaggagg gaccagacat ggatgtcgcc
     5521 atcacaggtt cgaacgggtt gatcgggagc gcgctcgcgc gctccctcga gcacgacggg
     5581 tatcgtgtgg tccgggtcgt gcggcccgga tcacccgccg tgagcgggga cgccgtgatg
     5641 tgggacccgc ggaacggcgt gatcgaccgc gacggtctcg acgggctcga cgccgtcgtg
     5701 cacctcgctg gcgcggggat cggcgaccgc cgatggaccc cggagcggaa ggaggagatc
     5761 ctccgctcgc gcaccgaggg aaccgcactg ctgacatcga cgctcgcatc ggtgccctcg
     5821 ccgccgagcg tgctcgtgag cggctcggca accgggtact acggcgacgg cggcggcgat
     5881 gagctcaccg agtcgagccc cgccgggcgc ggcttccggg ctgaggtcgt ggctgcgtgg
     5941 gaggctgccg cggctcccgc gatcgaggcg ggcatccgca cggtgttcgt ccggaccgcg
     6001 aacgtgctca caccgaacgg cgggctgctg ccgtacctgc tcacgccctt ccgcctcggt
     6061 ctcggcaccc ggttcgggag cggtcggcag tggttcccct ggatcacgct cgacgacgag
     6121 gtggcggtga tccggttcgc gatcgagtcg ccgaccctgc gcggaccggt gaacgctgcc
     6181 gccccggagc cggtgacgaa ccgcgacctc gtgacgatcc tgggacgcgt cctccggcgc
     6241 cccgcgccct ggatcgcccc ggcgctggtc ctgcgcctca tcgccggcgc cgagcgcgcc
     6301 gacgaggccc tgctctcaag cgcgaaggtc gtgccggccg cactcgagcg ggccgggttc
     6361 gcgttcggcg atcccgagct cgagccggcg ctccgtcgcc tcctcgcccc cgaggaggcc
     6421 cggacggcga gggtcccggt ccgatgagcg cggtacccgt cgagggcctt cacgagctcc
     6481 tgttcccggg cacgtcgcag acagacgtct cgagccggat cggacgcctg cgctggttca
     6541 acctcgcgat gggcgtcgtc cacgcggcgc aggccgtcct gatcctcgcg ctcgcgaccg
     6601 gcttctctat ccccatcgtg atccactccc tgaccggccc acccggatca tcggccgaga
     6661 cgatcacgct cttcgacctg ccgatcgcct gggcggtcgc ggcgttcctg ctcgtctccg
     6721 cgctcgccca tctcctcatc gcgtccgtgg ggttccgctc gtaccgacgc atgctgctgg
     6781 tcgggcggaa cgactaccgg tggatcgagt actcgatcac atggggagct ggccacatat
     6841 cctcgccgcg gttgagtcag cagagcgcgt ctgggtgatc ctcgccagcg ccacttcgga
     6901 gctcccgcca agctccggcg ggacgctctc acctggaatc ggattcgccc gctccggctc
     6961 attcgtcgtg gcgcacgtga gtggtccgtt cgcacctcgg ctacgtcgct cgagaacacc
     7021 atttgagcga ggcctgctcg tcccgcttgg ccgcgcggtt gacggatccg tcgtgcacat
     7081 ctcgatcacg ggtctggggc atgtggcggt ggtcggcgtc gatacatcag gcgttgtggc
     7141 gcagctcgtc ctggctgcag cgacgcaggg cggaccagag gagctgcgtc gcgccttcct
     7201 cggacagagc gaacgggtgg cgagcttgtg ccgcctgcct cacgtggtga cttgccgtcg
     7261 ttctgaggag gccgggcctg cgattcgaga gatcgagctc gagctcatgc gacgggctcg
     7321 tcacttcgtc gaggaaggcg tcgatgacgt ccgtgcccac ctcgccgagc attccgacga
     7381 gcaacttccc gcgatcatcc tcttattcga agagccgctc gcgacggaga tcggcatgat
     7441 cgacgcgctc ggccagcaag ccgcgaagtt gggagcagca gtgctgacgt cgggaagcgc
     7501 taccggcgcg gcgaggttgg tcgttcgcgc tgatcgctct ctcaagcttg agaccgatct
     7561 tcccctgccc tcgtctcttg agccactcgc gctcgacgac cccacgcagc aagaggccgt
     7621 cgaggtgatt cgcgaggcct atccctctga gccggccgag cccctcgagg agccgtctga
     7681 agcggtggcg gcagggcttc actccgggcc ttcaagcaag gcgattcaca ccgatctgac
     7741 cggcggccag ctcggccgaa gccgcaggtc acaagagtaa tcggggtttt tgacccccac
     7801 acgttactcg gcgctgtttc gcctcgagca cgttcccgtc gcattcatcg ctgatgggct
     7861 ggctcgccta caggtgcgct accccgagat cccggtcgtc ttcgcagact cgaggaagtt
     7921 cgcagaggaa tggacctatc gattcctcgc tgcggccctc gctgattcca tgggctcggg
     7981 catcgccagg tgaagcggcc tgcgactgtg cgagggtttt ggaccccttc acgtttgcgc
     8041 aggtagatcg cgtgccctcg gcaggaatcg aacctgcggc ccgcggttta ggaaacctcg
     8101 tgagcacgac gaccgagagt gactgagata cgtttcagca ggtagatgag ctggggcacc
     8161 cccaatcgac ctcggtcatt ggaccccggt ggacccaagg tggacccaag gtggacccaa
     8221 ggtcaggcca tgaactccac gcgatcggcg ctgcggtacg acggcgtagc ctgcggccat
     8281 gagacccgac gagctggagc gccgactccg ggaacgcctc gatgccctcg gccccgctcc
     8341 ccgcgccgag ttgctccacg ttgatgctcc cggacttcga ccgcgccgac cggatcggca
     8401 gctactgggg gaaccccaag acacgaacgt tcgcggagct gctgatcgac tgcgaggagg
     8461 accggacgct ccgggcggtg ctggtcggga tgctgcggga ggacgagcgg tcgccgtaag
     8521 gccttagcag ttccgaggaa ctctgcggcc attcccctac aggtctcgcc gctctcaggt
     8581 gttgaatctt tcgagatggg cgaggtggct cggctacgac gaaggtcgga ggctcccgag
     8641 gggtcgatgc cccccggaca ggagtcgccc agcttcgaga cgttcttcga ggacgagaag
     8701 gaccgcctgc tccgggtgct ctgcgtcatc accgggagcc ggggcgaagc cgaggacctc
     8761 gcgcaggagg cgttcacccg catgttcgag cggtgggatg aggtcgggca catggacgat
     8821 ctcgccggct acctccaccg gacagcgatg aatcttttcc gcagccagta ccggcgagcg
     8881 agggtcgcgc tgaagcgcgc cgctctatca acgccccagc gagacgtgtt cgaggcggta
     8941 gaggaacgag acgtcgcgac acaggcgctg gcccggctca cgccacgaca gcgcgccgcg
     9001 ctcgtcctga ccgaagcgct cggttactcc ggtgaggaga ccggccggct gctcggcatc
     9061 aaggcgtcga ccgtctgggc gctgacccac caagcgcgaa ccgctctgaa aggaaccctg
     9121 gaggcaaccg atgcctgaga cccgcacgct cctcgagcgc gtgatggagc gcgtcgagct
     9181 tcgccagttc acgctcgaag gattccacga ccgccgcgac cacaagcgcc ggaaccagcg
     9241 gatccgagca ggggtgctgg gcatcgcggt cttcgcgctc gcagcgatcg gactaatccg
     9301 gctcctcgga tccgagccca tcccggccga tcagccctcg agcgccttcg tgggcacgtg
     9361 gacctccacc gagctcgatg acgagagctc tcagacgatg accatccgcc cggcggagga
     9421 cggcgccctc gacatcacgc tgcagaacga ctcctccttc ctatgctcgg gtcgtcagac
     9481 ccgccttggt ggcgttgaca cgccgtcaac gatgaccggg accggacggc tcgaggacgc
     9541 taccaccctg gtggcgccct ccctccaggt atgcccctcc ggatgggccc tcgacatcgg
     9601 gccattgaca ttggagggga gagccaccag ctacacgctg gtcctcgacg tcgccaccga
     9661 cagactcttc gacaacctcg gggtggtgtg gcaccgagga gcaacaccgg agaacgcggc
     9721 ggacacgacg acggaatgcg aaatcttgga gccgcaggtg ggcagtcccg ggacttgttc
     9781 catgctccgg ggggaggtca ccttccgcgc cgccgagccc tggttcgatc acgtggactc
     9841 gtacatcgac ccgcgcctgt tcttcctgac atcgggtcct ggtctcccgg atcgccgggt
     9901 gaccatggat gcgatgatcg agatcctcgc gaacccgaac ccgctgccgg agggtccctg
     9961 ctaccgctat cctcggcttc cagcttctgc cgaggcgctg gtccaggcca tccgctccaa
    10021 ccccgacctc gaggcgacga tgcccgtcac cgaacgcgtc ggggggatcg atgcgctgcg
    10081 acttgacgtg gcccccgtcc caggggcatc cacgtgtccc aacggcgcgg tcccggtcgt
    10141 gtccacgtcc aacggtgggt gggggaccat gggccacggt agcctggggc gcctctacgt
    10201 cctggacctt cccggagggt ctgcccgggc cctggtgatc atgatcgaag ctccggaagc
    10261 ggacttcgag cgggcggtag aggcggcagc gcccgtggtg gactcgttcg agttccacac
    10321 ccgctgacgg cggtacttac gccgaggctc cggagtctcg accgcccccg ccagccagct
    10381 cgccgccgag cccgggttcc cagcgccgat cgaacgcgac ggacgaggcc gcttgtggaa
    10441 tcggcgcgag atccagtcgt gggcgagagc gtggcggaag gctcagccct ggcgctagcc
    10501 gaggaactcc acgcgatcgg agctgccggc gttcatgagc gcctgcagga gctcggtgtc
    10561 ctcggccgac agaatcgagc tcagctcgta gggggcagtg ttcggctggt gccagccgcc
    10621 ggatgtgtcg tgcacgtgca cggtcgcgcc cacggactcc agctcgctct cggtgagctc
    10681 ggcgcggccg ccgttcaggg cgcggtaccg ccccagctcg acgccgccca tcacgtccat
    10741 ctgccacagc tgcatcgggc gctcgcgctc ccacctgcgg aactcgtctc ggcgctttcg
    10801 ccgccggcgg agttcgatca gcccatggcg cttcaaacag cggtagatac t
//
LOCUS       DASUYD010000072         2784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_697192, whole genome shotgun sequence.
ACCESSION   DASUYD010000072 DASUYD010000000
VERSION     DASUYD010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2784
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697192"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VF195_04025"
     CDS             complement(<1..1067)
                     /locus_tag="VF195_04025"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HEX6580019.1"
                     /translation="MNTEFEILKSLESDLEEVASRERIRLQRATLQGSIRRNTGRSWM
                     KLAGVAAAFLVVAGSIGFLANMGVLGESAQNKFTQVGGAVAGSDGSVADSGGGAPEPA
                     QPNASPIPADDSEGAPGFGGFLEGESAGERDLHSGGQQQQDLSKIVRDGRIGIVVDDG
                     AFGDSVGELTFIAERHGGFVLSSSTSNERSGTFVLRIPERQFDQARNDIRGLATRVRF
                     EEVRGDDVTAEFIDFQTRLRILQTRRELLFDLFQQADTTDEILRMSSQLDDVTLRIEE
                     IQGQLRFLRDQVAESTLRVSIQERNAPAITGEPDVDKPDLGSSFDLGVQGFLRIVGAV
                     IVGLGYLIPITALAVAVWMAV"
     gene            complement(1064..1675)
                     /locus_tag="VF195_04030"
     CDS             complement(1064..1675)
                     /locus_tag="VF195_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580020.1"
                     /translation="MADQRSDSELLTASATEPGVFTELYRRHAEDLLRYFARRTLDPE
                     TAAELTAETFAQAFASRTTYRDTGANGVAWIYGVARHQLGRFFRAGRVDRAARRRLGM
                     PERDLPSADYERIEDLIDFAPIRGAIGEALESLRDDHREALRYRVIEQLSYPEVARRL
                     SCTEDNARQRVSRGLRRLGVVLQERGLQPATEPLTEPMTEKET"
     gene            1792..>2784
                     /locus_tag="VF195_04035"
     CDS             1792..>2784
                     /locus_tag="VF195_04035"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEX6580021.1"
                     /translation="MAEDSFPRQKARTRGFTLGAPRSFRVAEDGSRVSFIRSKAGDDP
                     EGCLWVFDVETGKERLIFDPSAVDGRQELTQDERDRRERMREQMTGVTTYDADDGLTF
                     APFVLGGRVHVADLLAGGAHPLEHAAEGAFDVRPDPTGRRVAYVVDGALHVQDLPARE
                     EPGGSSRVLAHDDDPGVQWGIAEFAASEELERHRGFWWSPDGERIAAARVDERPVRVW
                     HIASPVDPEVPPRAVRYPQAGTDNADVSLSILGLDGSRVDVDWDRDSLPYLVEVTWND
                     RCPLTLLVLSRDQKRWLVLEVDPDSGKTETLLEHTAEHWQTIVTGVPDRLSDGRL"
BASE COUNT          408 a          977 c          949 g          450 t
ORIGIN      
        1 cacacggcca tccacacggc cacggcgagc gcggtgatcg ggatcagata gccgaggccg
       61 acgatcacgg cgcccacgat ccgcaggaac ccctgcacgc ccaggtcgaa cgacgagccc
      121 aggtcgggtt tgtccacgtc cggctccccc gttatcgcgg gggcgttccg ctcctggatc
      181 gatacacgga gcgtcgactc ggccacctgg tcccgcagga atcgaagctg accctggatc
      241 tcctcgatcc ggagcgtgac gtcgtcgagc tgactcgaca tccggaggat ctcgtcggtc
      301 gtgtctgcct gctggaacag gtcgaacagc agctcccgcc gcgtctggag gatgcggagc
      361 cgcgtctgga aatcgatgaa ctccgcggtg acgtcgtcgc cgcggacctc ctcgaaccgg
      421 acacgcgtgg cgaggccacg gatgtcgttc ctcgcctgat cgaactgccg ctcggggatc
      481 cgaaggacga acgttcccga gcgctcgttg ctcgtcgatg aggagagcac gaagccaccg
      541 tgccgctcgg cgatgaacgt gagctcgccg accgaatcgc cgaacgcgcc atcgtccacg
      601 acgatcccga tccgtccgtc ccggacgatc ttcgagaggt cttgctgctg ttggccgcca
      661 ctgtgcaggt cccgctcgcc ggcagactcc ccttcgagga acccgccgaa gcccggggct
      721 ccttccgagt cgtccgcggg gatcggggag gcgttcggtt gtgcgggctc gggtgcgccg
      781 ccaccgctgt ccgcgacgga accatcgctg cccgcgacgg cgccaccgac ctgcgtgaac
      841 ttgttctgtg cgctctcacc caagacgccc atgttggcga ggaatccgat cgagccggcg
      901 accacgagga aggcggcggc gaccccggcg agcttcatcc acgagcgacc cgtgttccgc
      961 cggatggaac cctgcagcgt ggccctctgg agacggatcc tctcgcgcga cgccacctcc
     1021 tccaggtcgc tctcgaggct cttcaggatc tcgaactcgg tgttcatgtc tccttctccg
     1081 tcatcggctc cgtcaacggc tccgtcgccg gttgcagccc ccgctcctgg agcacgaccc
     1141 cgagccgtcg cagaccccgg ctcacgcgct gacgcgcgtt gtcctcggtg cacgacagtc
     1201 gccgcgcgac ctctgggtac gacagctgct cgatcacccg gtaccggagg gcctcacggt
     1261 ggtcgtcgcg gagggactcc agggcctcgc cgatcgcgcc gcggatcggc gcgaagtcga
     1321 tgaggtcctc gatccgctcg tagtcggccg acgggagatc tcgctccggc atccccaggc
     1381 gacggcgcgc ggcgcgatcc accctccctg cccggaagaa ccggcccagc tggtgcctcg
     1441 cgaccccgta gatccatgcc acgccgttcg ctcccgtgtc tcggtaggtc gtcctcgatg
     1501 cgaatgcctg tgcgaacgtc tcggcggtca gctcggcggc cgtctccgga tcgagcgtcc
     1561 gccgggcgaa gtagcggagg aggtcctccg cgtgccgccg gtacagctcc gtgaacacgc
     1621 ccggctcggt ggccgacgcg gtgaggagct cgctgtcgct gcgctgatcc gccaacgctc
     1681 ttccgatatc tggctccgat atccgttctc cagggagccg tgtcgggctc ctgtgacgtt
     1741 ccgctcgtac catgcctcgg atctcgaggc tgtgacaggg ggtagacggc gttggcggag
     1801 gactcgttcc cacggcagaa ggcacggacg cgagggttca cgctcggtgc gcctcgcagc
     1861 ttccgcgtgg ccgaggacgg atcgcgggtg tcgttcatcc gttcgaaggc gggagacgac
     1921 cccgaaggct gcctctgggt gttcgacgtg gagaccggga aggagcggct gatcttcgat
     1981 ccctcggcgg tcgacggcag gcaggagctc acccaggacg agcgggaccg gcgcgagcgg
     2041 atgcgcgagc agatgaccgg ggtcacgacc tacgacgccg atgacgggct gaccttcgca
     2101 ccgttcgtgc tcggaggccg cgttcacgtc gctgacctgc tcgcaggtgg ggcgcacccg
     2161 ctggagcacg ccgccgaggg agcgttcgac gttcggcccg atcccaccgg tcgccgcgtg
     2221 gcctacgtcg tggacggcgc tctccacgtg caggatcttc ccgcgcggga ggagcccgga
     2281 gggtcgagcc gggtcctcgc gcacgacgac gatccgggag tccagtgggg gatcgcggag
     2341 ttcgccgctt cggaggagct ggagcgccac cgtgggttct ggtggtcgcc ggacggggag
     2401 cggatcgctg ccgcgcgcgt ggacgaacga cccgtgcgcg tgtggcacat cgcctcgccg
     2461 gtggatccgg aggtgccgcc ccgagccgtt cggtaccccc aggccgggac cgacaacgcg
     2521 gacgtgtcgc tctcgatcct cgggctcgac ggctcgcgcg tggacgtcga ctgggatcgc
     2581 gactccctcc cgtacctcgt cgaggtgacg tggaacgacc gatgcccgct caccctgctg
     2641 gtcctgtccc gcgatcagaa gcgctggctc gtgctcgagg tcgaccccga ctccgggaag
     2701 acggagacgc tcctcgagca caccgcggag cactggcaga cgatcgtcac cggtgtgccc
     2761 gaccgccttt cggacggtcg gctc
//
LOCUS       DASUYD010000073         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_478342, whole genome shotgun sequence.
ACCESSION   DASUYD010000073 DASUYD010000000
VERSION     DASUYD010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478342"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..2209)
                     /locus_tag="VF195_04040"
     CDS             complement(476..2209)
                     /locus_tag="VF195_04040"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006561770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEX6580022.1"
                     /translation="MNLTTELERVEHDGTESGTGPASVPVPYRGHELLEQPLLNKDTA
                     FTERERRAFGLRGLLPPRVRTLEEQVALEIAKNRRKGDDLERFIGLAALQDRNEILFY
                     RLLLEHLEELMPIVYTPVVGHACQEYSHIMRRSRGLWLTPEDVDRIPELLANSGQDDV
                     RLIVATDNERILGLGDQGCGGMGIPVGKLALYTAGAGIHPSLTLPVSLDVGTDNRTLL
                     DDPDYAGYRQPRLRGPAYDGFIEAFVEGVIEVFPHAVLQWEDFKQHNAIRLLDRYRHR
                     ITSFNDDIQGTAAVVLAGIRSALRVLDEPLRAQRLVFLGAGAASIGIARLTRAQMRRE
                     GMPEDELRRAIVVLDSRGLTYLGRDPLDGDKEETALGPEEMTHYGFDAGESHGLDEVV
                     KRVAPSFLIGTSGTPGTFTEVAIREMAARARTPVILPLSNPTSKTEAQPTDILEWTDG
                     RAIVATGSPFPPVVRGERAHVIGQANNAFCFPGIGLGAVVAEAREVTDEMFLSAADAL
                     ASVVSSERLEQGSLYPNQSELRVASRAVAIGVARAARDGGVGRNLWDDEVESAVDAMM
                     WEPRYLRYEPA"
     gene            complement(2206..3612)
                     /locus_tag="VF195_04045"
     CDS             complement(2206..3612)
                     /locus_tag="VF195_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX6580023.1"
                     /translation="MIDLMNDTALPLAYLFAAACFVLALKWLSHPTTARRGVKTGELG
                     MLVAVVATLLEFEIVEYQWIATAIVAGVALGVPLGLLVPMTAVPQRTALSHAFGAVAA
                     GLVGTAEFYLQRPAIDETTMVAIGLEVVLGFLTFTGSLVAAGKLQGMIRDRPWVYRGQ
                     NIVNLSILATALLMVAALVARPGAAFLFPIAVALSLVFGVLLVIRIGGADMPTVISLL
                     NGFAGLSASMMGFVLHNNLLIVAGALDGSSGLFLSIVMCKAMNRSFVNVLFGGFGAAS
                     SEGAVEQRPVRSVDPEEAAFLLDAARSVVIVPGYGMAAAQAQHKVRELTDALLKRGVD
                     VKFAIHPVAGRMPGHMNVLLAEANVPYDQLYDLDAINPEFPQTDIVLIVGANDVVNPA
                     ARTQTDSPIYGMPILDADAAASIIVVKRSMRSGFAGVDNELFYLDKTQMLFGDAKKVI
                     GGVVDELSKLPAREVAAA"
     gene            complement(3609..3938)
                     /locus_tag="VF195_04050"
     CDS             complement(3609..3938)
                     /locus_tag="VF195_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEX6580024.1"
                     /translation="MTETLEVSLFIFVLASFLGYAVITRVPGLLHTPLMSLTNALSAI
                     SIVGSLILAGAGEAAFATILGTIAVFASTTNIVSGFLITDRMLKMFKRREAPKGPAGE
                     PPKGASA"
     gene            complement(3931..>4337)
                     /locus_tag="VF195_04055"
     CDS             complement(3931..>4337)
                     /locus_tag="VF195_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HEX6580025.1"
                     /translation="VAGVDVVITTALIPGAPAPTLITADAVAGMRPGSIIVDCAAERG
                     GNCELTRADETVVTDNRVTILGPTNLPSTIPHDASLMYSKNIAALLGLIVRDGRLALD
                     EEDEIVRGALVTRGGEIVNRRVREALEEVRDD"
BASE COUNT          725 a         1479 c         1472 g          661 t
ORIGIN      
        1 acgatcgctc tccctcgccg ctcgcgaagt cgaacccaca tcccggacac tggcggaatc
       61 cctcccacct cgccatctcg atcgcctccg ggtccattag aagcccacgg cacgaccgga
      121 tgcagagccg aagggccttg cggtggcggg gcttccgacc cagtccgcag atccgcggaa
      181 ggggtcgccg ttcagcatga tcaggggccg agttgccgag cccggtcctc acggcgaagc
      241 agagagcgcc gtcctcaacg gctccgatcg aggccgcgtg agccctgaac gggaccaagg
      301 cctccggctc gcttcgaagc gctcactggg cccgtcggag gcgcgtccgc gggacgagcc
      361 gccaggcgag cgcgaacgag cccacgaatg cccagcttcg cgacgaggag cgacttgaca
      421 tcgccggggg atcgatgggt cgcagcgcga gcgcgagacg agtagaggtc ggagcctagg
      481 cgggctcgta ccgaaggtag cgcggctccc acatcatcgc gtcgaccgca gactcgacct
      541 cgtcgtccca cagattccgg ccgaccccac cgtcccgcgc ggcccgcgcc accccgatcg
      601 cgacggcgcg cgaggcgacc cgcagttcgg actggttcgg gtagagcgag ccctgctcga
      661 gccgttccga ggagaccaca ctcgcgagcg catccgctgc ggataggaac atctcgtcgg
      721 tgacctcgcg cgcctccgcg accaccgccc cgagtccgat ccccgggaag cagaacgcgt
      781 tgttcgcctg gccgatcacg tgggcccgct cgccgcggac gaccggtggg aaggggctgc
      841 cggtcgcaac gatcgcgcgg ccgtcggtcc actccaggat gtccgtcggt tgtgcctcgg
      901 tcttggaggt ggggttcgac agcggcagga tcaccggggt tctcgcacgg gccgccatct
      961 cccggatggc cacctccgtg aaggtcccgg gcgtaccgct cgtgccgatg aggaagcttg
     1021 gggcgacgcg cttcacgacc tcgtcgagcc catgcgactc gccggcgtcg aatccgtagt
     1081 gcgtcatctc ctccgggccg agcgcggtct cttccttgtc gccgtcgagg ggatcgcggc
     1141 ccagatacgt gagcccacgg gagtcgagca ccacgatcgc ccgccgaagc tcatcctccg
     1201 gcatgccctc tcggcgcatc tgcgcgcgcg tgaggcgcgc gatgccgatg ctggccgcgc
     1261 cggcccccag gaagacgagc cgttgggctc ggagcggctc gtcgagtacc cggagggccg
     1321 atcggatccc cgcgagcacg accgccgcgg tgccctggat gtcgtcgttg aagctcgtga
     1381 tccgatggcg gtaccggtcg agcaagcgga tcgcgttgtg ctgcttgaag tcctcccact
     1441 ggagcacggc atgagggaag acctcgatga cgccctcgac gaaggcctcg atgaaaccgt
     1501 cgtacgccgg gccccgcaga cgaggctgcc ggtagccggc gtaatccggg tcgtccagca
     1561 gcgtccggtt gtcggtccca acgtcgagcg agacgggaag ggtcagggac ggatggatcc
     1621 cggcgccggc ggtgtagagc gccagcttgc caaccgggat ccccatgccc ccgcagcctt
     1681 ggtcgccgag acccaggatc cgttcgttgt ccgtcgccac gatcagccgg acgtcgtcct
     1741 gtccggagtt cgcaagcagc tcggggatcc ggtccacgtc ttcgggcgtg agccagagac
     1801 cgcgcgaccg acgcatgatg tggctgtact cctgacaggc atggcccacg accggcgtgt
     1861 acacgatggg catgagctcc tcgaggtgct cgagcaggag ccggtagaaa aggatctcgt
     1921 tgcggtcctg gagcgctgcg agcccgatga accgctcgag gtcgtcaccc ttgcggcgat
     1981 tcttcgcgat ctccagggcc acctgctcct cgagggtccg aacgcgcggc gggaggagtc
     2041 cacggaggcc gaaggcgcgc cgctcccgct cggtgaacgc ggtgtctttg ttcagcagcg
     2101 gctgctcgag caactcgtgt ccacggtaag ggaccggcac cgatgccggg cctgtgcccg
     2161 attcggtccc atcgtgctcc actcgttcca gttccgtcgt gaggttcatg ccgccgccac
     2221 ctctctcgcg ggcagcttgg agagctcatc gacgactccg ccgatcacct tcttggcgtc
     2281 gccgaacagc atctgcgtct tgtcgagata gaacagctcg ttgtcgacgc cggcgaaccc
     2341 cgagcgcatg gagcgcttca cgacgatgat cgaggcagcg gcgtccgcgt cgaggatcgg
     2401 catcccgtag atcgggctgt cggtctgggt gcgcgcggcc ggattcacga cgtcgttcgc
     2461 gccgacgatg agcacgatgt cggtctgcgg aaactccggg ttgatcgcgt cgaggtcgta
     2521 gagctggtcg tacggcacgt tcgcctcggc aagcaggacg ttcatgtggc cgggcatgcg
     2581 tccggccacc gggtggatcg cgaacttgac gtcgaccccg cgcttcagca aggcgtcggt
     2641 cagctctcgg accttgtgct gcgcctgcgc ggcggccatc ccgtagccgg ggacgatcac
     2701 gacggaccgg gcggcatcga gcaggaaggc ggcctcctct ggatccacgc tgcggaccgg
     2761 ccgttgctcg acggcaccct ccgacgacgc ggccccgaag ccgccgaaca gcacgttgac
     2821 gaaggatcgg ttcatcgcct tgcacatgac gatcgacagg aacaggccgc tcgacccgtc
     2881 gagggctccg gcgacgatca gcaggttgtt gtgcaggacg aagcccatca tcgaggccga
     2941 caacccggcg aagccgttga gcagcgagat caccgtgggc atgtcggcgc cgccgatccg
     3001 gatcacgagc aacaccccaa acaccagcga gagcgccacg gcgatcggga acaggaacgc
     3061 cgcaccggga cgggcgacca gagccgcgac catgaggagc gccgtggcga ggatcgacag
     3121 gttcacgatg ttctggcctc ggtagaccca ggggcggtcc cggatcatgc cctggagctt
     3181 gcccgcggcg accagcgacc cggtgaaggt caggaacccg agcacgacct ccagtccgat
     3241 cgcgaccatc gtggtctcgt cgatcgccgg gcgctgcagg tagaactcgg cggtcccgac
     3301 gagcccggca gccacggctc cgaacgcatg cgacagcgcg gttcgttgtg gcaccgcggt
     3361 catcgggacc agtagaccaa gcggaacgcc gagcgccacg cccgcgacga tggcggtcgc
     3421 gatccactga tactccacga tctcgaactc caacagcgtg gcgacgaccg cgacgagcat
     3481 gccgagctcg cctgtcttga cgcctcgacg agcggtcgtc ggatgcgaca gccacttgag
     3541 ggccaagacg aagcacgcgg cggcgaacag ataggcgagc ggcagagcgg tgtcgttcat
     3601 gaggtcgatc atgccgacgc ccccttcggt ggctcacctg ccggtccctt tggcgcctca
     3661 cgccgcttga acatcttcag catccggtcc gtgatgagga agccggagac gatgttggtc
     3721 gtcgacgcga agaccgcgat cgtccccaag atggtcgcga acgccgcttc gccagcgccg
     3781 gcgaggatga gcgaccccac gatcgagatc gccgacaggg cgttggtgag cgacatcagg
     3841 ggcgtgtgca gcagcccggg cactcgggtg atcaccgcgt agccgaggaa cgacgcgagc
     3901 acgaagatga acaggctgac ctcgagcgtc tcagtcatct cgcacctcct cgagcgcctc
     3961 acggacccgt cgattcacga tctccccgcc cctggtcacg agcgcgccgc gcacgatctc
     4021 gtcctcctcg tccagtgcga gccttccgtc cctcacgatg aggccgagca gcgccgcgat
     4081 gttcttcgag tacatgaggc tcgcgtcgtg ggggatcgtc gagggcaggt tcgtgggtcc
     4141 gaggatcgtg acgcggttgt cggtgacgac cgtctcgtcg gcgcgggtga gctcgcagtt
     4201 gcctccccgc tcggcggcgc agtcgacgat gatcgagccc ggtcgcatcc ccgcgaccgc
     4261 atccgccgtg atcagcgtcg gggcgggggc accggggatc agggccgtcg tgatgacgac
     4321 gtcgacgccg gcaacga
//
LOCUS       DASUYD010000074         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1580419, whole genome shotgun sequence.
ACCESSION   DASUYD010000074 DASUYD010000000
VERSION     DASUYD010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1580419"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..94)
                     /locus_tag="VF195_04060"
     tRNA            complement(19..94)
                     /locus_tag="VF195_04060"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Asp,seq:gtc)
     gene            complement(111..183)
                     /locus_tag="VF195_04065"
     tRNA            complement(111..183)
                     /locus_tag="VF195_04065"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(147..149),aa:Glu,seq:ttc)
     gene            complement(262..777)
                     /locus_tag="VF195_04070"
     CDS             complement(262..777)
                     /locus_tag="VF195_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580026.1"
                     /translation="MRIARALVLLLLLAAACGGDADAGPAPGDYFAQLQRVSETAHIQ
                     ERGLRRDLRVRLQEATPEEDRKAVVSVFLDQSARLDQDVLDALGALVPPPELVAPQRA
                     FFDAWKSSLDLIVKVRDASFPTADEVLERLEAPVFRNAAAEIRARCDDLQAAVVAAGS
                     DADLVCDGRSS"
     gene            856..1035
                     /locus_tag="VF195_04075"
     CDS             856..1035
                     /locus_tag="VF195_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580027.1"
                     /translation="MPHREVRVVLEWLGLIEPDRARHEPAALPSWAPWVVTFLLVGAA
                     AIGALVVSVVLELRG"
     gene            1459..2049
                     /locus_tag="VF195_04080"
     CDS             1459..2049
                     /locus_tag="VF195_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012248546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580028.1"
                     /translation="MNKEQLDELLYQSLETERGGVKIYETAVRCAQNDDLKKEWGKYL
                     DETRNHEVIMLRVLDAFGLDAETETPGRRIVRSKGEALVASMETALGADPAAAQLVAA
                     ECVVEAESKDHMNWELIGEAAKKLTGDEGKALKEAHGEVEEQEDEHLYHTMGWARELW
                     LESLGLPAVLPPPEEQKHTKSAIGADRAKQARTEML"
     gene            2079..2435
                     /locus_tag="VF195_04085"
     CDS             2079..2435
                     /locus_tag="VF195_04085"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX6580029.1"
                     /translation="MTEESATTRAHASFADFSTLVARWTGNHWAFLVAAALVVVSLLL
                     FGVEITNIAISIGTLLMVFILQNTQNRDSAALHLKLDEMVRVEPAARDDVRGVESRPA
                     HEIDELHLHEPDGSKV"
BASE COUNT          432 a          872 c          901 g          381 t
ORIGIN      
        1 tccgactcgc tcacccagcg cgcccgacgg gcttcgatcc cgcgatctcc tccttgacag
       61 ggaggtgagg acgaccggac tcctctacgg gcgcatatgt ggtggtgccg tgcccccaac
      121 gggattcgaa cccgtgctac cgccttgaaa gggcggcgtc ctaggccgct agacgatggg
      181 ggcctgcccc acgccatctt cccatcgggg acagctcaga gagtatagcc aggcccctca
      241 ggccggtgat cgagctccct gtcaggacga ccgaccgtcg cacacgaggt ccgcgtcgga
      301 gcccgctgcc acgacggccg cctgcaggtc gtcgcagcgt gcccggatct ccgcagccgc
      361 gttgcggaag acaggggcct cgagccgctc gagaacctcg tcggcggtcg ggaagctcgc
      421 atcgcggacc ttcacgatga gatcgaggga ggacttccag gcgtcgaaga acgctcgctg
      481 gggggcgacg agctccggag gaggaacgag cgctccgagg gcatccagga cgtcctgatc
      541 gaggcgggcg ctctggtcga ggaagaccga caccaccgct ttgcggtcct cctccggcgt
      601 cgcctcctgg agccggaccc ggaggtcgcg gcggagccct cgctcctgga tgtgagcggt
      661 ctcgctgacc cgctggagct gggcgaagta gtcgccgggg gcgggcccgg catcggcgtc
      721 cccaccgcag gctgcggcca ggagcagcag caggacgagg gcccgcgcga tccgcatgtc
      781 cggcagcgtg tcagcgcggc gacaggaacg gcaaccggcc ctcaaggagg gcctccgttc
      841 gcccgaagct gtcgtatgcc ccatcgcgag gtccgcgtgg tcctggagtg gctcggcctg
      901 atcgagcccg atcgcgcgcg gcacgaaccg gccgcgctcc cgagctgggc tccgtgggtg
      961 gtcaccttcc tgctcgtggg tgccgcggcg atcggggccc tggtcgtcag cgtggtcctc
     1021 gagctgcggg gctgaatcgg tcgccgaccc tcggcgccca accatcggcg cactccagag
     1081 cgtgacgcgc gatcggtgac cgggccctga cgatcctccc tcgcgggcat ctgcggacct
     1141 caagcacctc catcgttggg ccgacgaagg cgaccgtgat cgtctgccgc agcccgaacg
     1201 tctggatgct gcggcatcga cgcaacagta gcgcctcgcg gcgcccgagc ggtgggtgac
     1261 cccggagtcc ccgcatccgc tccctccgtg tctccgggac gagaacgcgc cacgcgccgt
     1321 ccgggccggc catctcgacc agctgcatcg gacgaccata gcgaccgtgg ccgacacagc
     1381 gtttccgccg accgcgcccc gggtagtcac cctctgggtc ccgtctgggc ccgttccgac
     1441 gtcgatggag ggaagtcagt gaacaaggaa cagctcgacg aactcttgta tcagtcactc
     1501 gagacagagc gcgggggggt caagatctac gagactgccg tcaggtgtgc tcagaacgac
     1561 gacctgaaga aggagtgggg caagtacctg gacgagaccc gcaaccatga ggtcatcatg
     1621 ctgcgtgtcc tcgatgcgtt cggcctcgac gcggagaccg agacgccggg acgccggatc
     1681 gtgcggagca agggtgaggc gctcgtcgcg tccatggaga ccgcgctcgg ggccgatccc
     1741 gccgcggcac agctggtcgc cgccgaatgc gtcgtcgagg cggagtcgaa ggaccacatg
     1801 aactgggagc tgatcggcga ggcggccaag aagctcaccg gtgacgaggg gaaggcgctc
     1861 aaggaggccc acggcgaggt ggaggagcag gaggacgagc acctctacca cacgatggga
     1921 tgggccagag agctctggct cgagtccctc ggcctgcctg cggtgctccc gcctccggag
     1981 gagcagaagc acacgaagtc cgcgatcggt gccgaccgag ccaagcaggc acggacggag
     2041 atgctgtgat cgatgggcag acgtcacgca gggagcccgt gacggaggag tcggcgacga
     2101 cgcgcgcaca tgcgtcgttc gccgacttct ccacgctggt ggcgagatgg acggggaacc
     2161 actgggcgtt cctcgtcgcg gccgcgctcg tggtcgtgtc cttgctgctc ttcggcgtgg
     2221 agatcacgaa catcgcgatc tcgatcggga cgctcctgat ggtgttcatc ctccagaaca
     2281 cccagaaccg ggactcggcc gccctgcacc tgaagctcga cgagatggtc cgggtggagc
     2341 cggcggctcg cgacgacgtc cggggcgtcg agtcacgacc tgcccacgag atcgacgagc
     2401 tccatctgca cgagcccgac ggatccaagg tctgatgtga agtcccacca cccgtgtccc
     2461 ggaatggctc cggcacctat gcgacatctc gggccacctt cctcggcctg cctccaggat
     2521 tggcgagcgc cccgaactcc ttgagcttgt catggcgggc tcggtgagtg cgcagcagca
     2581 ggccgt
//
LOCUS       DASUYD010000075        24220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_258250, whole genome shotgun sequence.
ACCESSION   DASUYD010000075 DASUYD010000000
VERSION     DASUYD010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24220
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258250"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF195_04090"
     CDS             complement(<1..855)
                     /locus_tag="VF195_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX6580030.1"
                     /translation="MSATDRTIGTAKDLASLGAPSRWYQDAVIYELHVRAYQDSNADG
                     VGDLAGLVQRLDHIRDLGATAVWLLPFYPSPLKDDGYDISAYKAIHPAYGTMRDFRTF
                     LKEAHRRDLRVITEVVMNHTSDEHPWFQRARSAKPHSWQRNFYVWADTPEGYPDARVI
                     FSDFESSNWAWDQVAGQYYWHRFYSHQPDLNFDESRVRAAMFDVVDFWLELGVDGLRL
                     DAVPYLFEREGTNGENLPETMQFLSDLRRHVDASFEDRMLLAEANQWPEDAVAYFGNG
                     DRCHMAFHF"
     gene            complement(909..2894)
                     /locus_tag="VF195_04095"
     CDS             complement(909..2894)
                     /locus_tag="VF195_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HEX6580031.1"
                     /translation="MERGVESATLTRIVIEHVSPAVDGGLFAAKTSVGDVVPIEADVF
                     TDGHDLPAARVLIRHDDADWGEAPMVPLGNDHWRSDLAIMEQGTYRFRIEGWVDTWAT
                     WRSDLEKRRAAGQDVAVELREGAALVSGAVRRARAEGADADADRLKQAASIMRRKDTA
                     AGTTLALSDDLHALMSSHPDRRRGTTVSPELEMVAARERARHGAWYELFPRSASPDPA
                     RHGTLRDVAAVVPDIAELGFDVLYLPPIHPIGRTSRKGPNNTAAAGPEDPGSPWAIGS
                     EAGGHTAIHPELGTFDDLEHLLAELKRHEMELALDLAFQCSPDHPWVTQHPEWFRHRA
                     DGTIRFAENPPKTYEDIYPIDFETKDREGLWRALLDVVLFWVDRGIPIFRVDNPHTKP
                     FAFWEWLLAEVKRDHPDVLFLSEAFTRPRVMEHLAKIGFDQSYTYFAWRRTKDELTQY
                     LIELTQGPVRDFLRPNLWPNTPDILTDQLQEGGPPAFRLRYLLAATLGANYGIYGPAF
                     EHMEREPREAGSEEYLHSEKYEIRPRTPAAGGLRELIARVNLIRREHPALRHDRTLRF
                     HPVDGDQLLAYSKTLPDGKDPVVVVVNLDPVHPRSGWVDLRMPELGMAWDAPFSARDL
                     LGGETFSWTGARNFVELHPGHQPGHVLAIEPGEGRTP"
     gene            2942..3730
                     /locus_tag="VF195_04100"
     CDS             2942..3730
                     /locus_tag="VF195_04100"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6580032.1"
                     /translation="MKRFAVASIGLSLVLAAACSQTLAGDPTERPPEPFIDERASESP
                     APGVSDVAGGEETADPATERGRPRFEGGVFRIGPTLRDVLIGRNWTPGCPVAIHDLRV
                     VHVSYWNFRGGVGVGPLVLHERVARDVLWVFQRLFRAKFRIQDIELAAKDRPITREDY
                     WNKTRRSVTASFNCRPATDSTTLSQHSYGWAIDINPLQNPYVRSDGSTLRHIAQPFKD
                     RSLQHMGMIHDSDIVVGSFAAIGWEWGGHWHTLKDYMHFSLTGR"
     gene            3879..4022
                     /locus_tag="VF195_04105"
     CDS             3879..4022
                     /locus_tag="VF195_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580033.1"
                     /translation="MTIRTHLAAFGLAALGQLRGILEAPSTYRTAVLRALMVRATAPT
                     SPR"
     gene            4151..4621
                     /locus_tag="VF195_04110"
     CDS             4151..4621
                     /locus_tag="VF195_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580034.1"
                     /translation="MSEEVRIRPVASDDLPIFFEYESDPEAAKMAVFESRDRASFDEH
                     WAKTMRDERNIVRAIEAAGVVVGNIGSWEHDGERYVGYWIGREHWGRGMATAALAALV
                     AELTDRPLFAHVADTNAASIRVLEKSGFRRIGEPTADADADGVVELHYRLDEAG"
     gene            5003..5599
                     /locus_tag="VF195_04115"
     CDS             5003..5599
                     /locus_tag="VF195_04115"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6580035.1"
                     /translation="MRGHTIRRAMLMVLGVMLFVSASPHVALGSGGGGCGRAVTDADG
                     TSVRIRNFCFGPTVLRVPEGETVTWVNRDDFPHVVLGANAAWGGYGKVRGGGEVGYRF
                     VNSGVYPYVCTYHPGMIGAVVVGNGKPDGGAYTVTTNAGPVIQAESSQDAMEPQAVPV
                     AVTPTAWAPAAWVIGLLVVVAAAIAASRRRRAHGTVTD"
     gene            5618..6823
                     /locus_tag="VF195_04120"
     CDS             5618..6823
                     /locus_tag="VF195_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012874115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6580036.1"
                     /translation="MTPSWRFRSTLAIAAMLLIGACGNAGAPSDTTGPSPSAPSSTAG
                     PSSPSPNLADVRIRLEQVATLEQPLAMAVREDDSALYVAQKTGTVVAIRAARTVDKVP
                     VLDLSDEVSLGGEQGLLGLAFAPGGRELYVNYTDTNGDTHVTGFAMRGDRADPATRRD
                     ILVVDQPYSNHNGGNLVFGPDGYLYVGLGDGGSGGDPLGNGQSLSTLLGKMLRIDPQL
                     SGGAPFRIPADNPFVGRGDARPAIWAYGLRNPWRYSFDRVTGGLWIGDVGQTDWEEID
                     VQPAGSAGGENYGWNILEGSHPFFGEELPDGVAPTYEYSHAEGGCTVIGGYVYRGEAI
                     PALYGAYLFGDLCIGEIEALGVENGRVREHRVLGPVVENLSSFGEDADGDLYAMSLSG
                     GVYLITADS"
     gene            6873..8051
                     /locus_tag="VF195_04125"
     CDS             6873..8051
                     /locus_tag="VF195_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6580037.1"
                     /translation="MSGDIRPSQKAVSFTESVIREMTRLAMLHDAINLSQGFPDFAAP
                     EEVKEAARQAIADDVNQYPITWGAEGFRRELARNYRERYGMDWVDPERDITVTCGSTE
                     AMIAAMLGVLDPGDEVVVFEPFYENYGPDTIISGAVPRTVTLHSPDWTFDEAELAGAF
                     NERTRGIVINTPNNPTGKVFTREELDVIGGLCQRWGVVVFTDEIYEHILYDGAVHVPP
                     GAVPGLEDRTVTISALSKTYSVTGWRVGWVVAPAPLSRGIRSVHDFLTVAAPAPLQEA
                     GEVALGLPEEYYRRTADEYRERRDLWIETLRETGFKVEPPAGAYYVMAEISGFGFPDD
                     VRFARHMVEDVGVAVVPGSSFFSRPELGAHLVRFAFCKKLETLEVAGERLRGLHPRVA
                     "
     gene            complement(8379..9425)
                     /locus_tag="VF195_04130"
     CDS             complement(8379..9425)
                     /locus_tag="VF195_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6580038.1"
                     /translation="MDLITPSTKEDVVGALRSASERRARVAIVGGRTHADKGNRCEVD
                     EELAMAGLDRVISHDPEEMLAVVESGLSVGALRQVLAQGGQEWPSDAPDDATVGGTIA
                     VAASSPRRLRMGPLRDSVVELEIVTGDGRLVRSGARTVKSVQGYDLHRLATGSLGTLG
                     AIVQVAVKVRPLPKARRLVVSAAGGLLAGRTLLDAVPLPSSVVATSDRIEIALEGWPE
                     EIEEQTATIRGTAGDVEIHEGTTVDGPEAVAAPIVAEVAVAPSRLDAVLGGADDWRAL
                     MGIGIAWVGLPDGDRLASLRTRVAGAGGIAPVIRGPGGLGDAPVAALEVHRRLKAAFD
                     PSGILAPGRFWGGL"
     gene            complement(9416..10909)
                     /locus_tag="VF195_04135"
     CDS             complement(9416..10909)
                     /locus_tag="VF195_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX6580039.1"
                     /translation="MADLDAARRELEEALGPDEVLSDPLALRLYARDASMVEGAAGLV
                     VFPRSTDDVAACVRAAAMHDLAVVPRGSGTGLAGASTPIGDALVVVTSKMHSILEIRP
                     EDRLAWVEPGLFNLDLGVALRGTGFTYMPDPSSQQVSSIGGNVGTNAGGPHCLAYGVT
                     SAHVLALDVVLGDGTVTRLGSEGPEAAGYDLRGVVVGSEGTVGLVTAACVRLTPEPPA
                     ITTMLLDFATVEDCAATVSDIIARGVVPAALEMMDQGIVRAVESFAHAGYPTDAAAVL
                     LVEVDGIRAGVDAEEREVGAAARDHAVGTIRVAESPEERALLWKGRKSAFGAIAQIAP
                     HYHLHDCVVPRTKLVEVLSGVYTICRREAITVTNVFHAGDGNLHPLLSFDRSEPGVLE
                     RVLRTSEEIVRLCVASGGSLSGEHGIGLEKRDFMPLVFTEDDLTAQACVRSAFDPSGR
                     MNPQKVLPDGARCGDFVVAAQIQAATQAGSAAGVGDAARNLPEGSWI"
     gene            10929..11405
                     /locus_tag="VF195_04140"
     CDS             10929..11405
                     /locus_tag="VF195_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015032875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580040.1"
                     /translation="MATWAEFELAEPELAAFGKERLDGQVCFHATLRTDGSPRVHPVE
                     PWIAVGLLLVRFRGHSPKVDEVLRDGRYALHSPMDNPDGVGGEFMVRGWMEQIGDTHP
                     AVQRFVHDATYPLAFYAMSVEEAVGTTYEGEGTEPVYRRWRPHATRPGEPSGAEKS"
     gene            complement(11360..11695)
                     /locus_tag="VF195_04145"
     CDS             complement(11360..11695)
                     /locus_tag="VF195_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX6580041.1"
                     /translation="MADLSTVEGVHESLDDIQLVDCREQYEWDAGRVTGALHIPLNDV
                     MAGVGADVLDIAKPVAVICRSGNRSELASMMLQARGYDATNVEGGMEAWAAAGYDFSA
                     PDGSPGRVA"
     gene            complement(11730..12242)
                     /locus_tag="VF195_04150"
     CDS             complement(11730..12242)
                     /locus_tag="VF195_04150"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012834970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HEX6580042.1"
                     /translation="MPRIVLVEGDITEQRVDAIVNAANATLLGGGGVDGAIHRRGGPS
                     ILEACRRIRSTEWPDGLPTGRAVATTGGDLPARWVIHTVGPVYAREEDPVTLLSSCHT
                     ESLRVADELEAARVAFPAISTGVYGYPIDEAARVAVEAVRTADTSVHEVRFVLFDATA
                     HAAFQRAMTG"
     gene            12260..13198
                     /locus_tag="VF195_04155"
     CDS             12260..13198
                     /locus_tag="VF195_04155"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEX6580043.1"
                     /translation="MIVCLAANPSVDRLFEVERLVKGDIHRPSGFVQVAGGKGLNVAR
                     AARSLGADVCAVALLRGHTGRWLQESLAAEGVKGEFVWTHGESRSSLSVADRETAGLT
                     EFYEHGAVAPNAAWIELTHAAAMRWERGSWLTISGSLPRGAPDEGYRDLIEEARRGGV
                     RVALDSEGERLRLALTAEPDIVKVNVAEAGELLGVPTARRDDSLAAASKIRNMAGGDG
                     HAGIVTRGAEGVMLAAPDGTLYEGVLYVRGRYPVGSGDAFLAGLITALERGNDWEDAL
                     RVALGAGAANAELAGAARIDPSRAAALARQADVHLL"
     gene            13266..13700
                     /locus_tag="VF195_04160"
     CDS             13266..13700
                     /locus_tag="VF195_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580044.1"
                     /translation="MLDSPDAFGSTYGEESSLSEPAWRDRAGGRWRGGTAVVLAGRDQ
                     TGALVGTATGAEFEAELGVAHLYAMWVAPDARGTGVGRALVEAVVAWARGRGCGRLVL
                     SVTETNRGARGFYEACGFADTGERRPLREGSDLDVLIFAKTF"
     gene            13757..14983
                     /locus_tag="VF195_04165"
     CDS             13757..14983
                     /locus_tag="VF195_04165"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580045.1"
                     /translation="MKRPTGADISPAAAVLGLFLTFGLIVASFFPFLSIYFAGRGFDP
                     SQIGFLLGAMAAVRIVAMPIWGHVADTRIGRRRALQLGLLGMVAFAIVASVLDSLVGV
                     AFGAVGIALAMAASGPNIDALALVQLGDERMADYGRIRGWESLSYAAGCLAFGWLLDL
                     YGVSLALPLYAGAGAVMLVWTTIAVRPDRPTQPAQHGRLGAVGAVFRTAPRMWGFLVA
                     TLLVWTGFNGAWNFVGLKIEEPLLIGVGTALGGLTEVGVMRLSSRLHRRLGLRKVYAL
                     GCLVYATGFLLWGLVESRTIVSLLAVFEGAGFSLLFTTSILVVGKLLPSTLYSTGNSI
                     GAMVGFGIGPIIGAGLGGVVYGRLGSTTLFIGSAVLALLGGVAAMIALRIPQLDTPLR
                     EPLPTGDAVAPEPGRL"
     gene            15094..15387
                     /locus_tag="VF195_04170"
     CDS             15094..15387
                     /locus_tag="VF195_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580046.1"
                     /translation="MTNNPNGSDEQPIAGLVDKAKEVAETVAEKAGPAMEKAKEVAGT
                     VAEKAGPVVDKAKEVAGTMVEKAGPAMEKAKESATELVDKAKELLATKDDDAK"
     gene            15434..15667
                     /locus_tag="VF195_04175"
     CDS             15434..15667
                     /locus_tag="VF195_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6580047.1"
                     /translation="MVSAYILIQTEVGKAAQVTQQVSGIDGVVAVDPVTGPYDVVARA
                     EADDLDGLAKGTVMPMQELDGITRTLTCPVIQL"
     gene            15693..16400
                     /locus_tag="VF195_04180"
     CDS             15693..16400
                     /locus_tag="VF195_04180"
                     /inference="COORDINATES: protein motif:HMM:NF014708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6580048.1"
                     /translation="MTITIRRDAEISDVDGGWFRARWHFSFDTYHDPAYMRFGTLRVF
                     NDDRLVPGAIWPMHPHEDIEGLTYVVEGSFRHQDDVGGAPGPLPAGSVQRMTLGRGAW
                     HSEQNASETEPMRFIQMWIMPAERGLEPGVEQKVFTEEDRTDRLLRVISGDGGDAVLV
                     HQDAHVFVSRLRSDSDATHALGEGRGVYLYLIEGDADVAGERMTTGDAAEIEDEPSIP
                     IHANAVTELIAVDVALD"
     gene            complement(16401..17609)
                     /locus_tag="VF195_04185"
     CDS             complement(16401..17609)
                     /locus_tag="VF195_04185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6580049.1"
                     /translation="MPNRDSADLVVVGAGTIGGWASVFATEHGAERVVVLEKGEAGQG
                     ASSRAAGVVRAQGGTPETVALGMWSIDFYRSQRERYGTDSGFRELGYLILATDEAQER
                     EANARVEMQRGAGLGDVRYVDAAGAARLNPTLDPATFRGGTYAPGDGCVDPPRNVRAY
                     SIAMQRAAVELRERTSFLGIRTGSDGAVTGVETPEGTIATERVLLTGGPKLREVGALV
                     NARFFVGAVRHQVAVTEPHEAFDVGRQPMVFENAIGLYWRLEEGGLLFGMSNPDEAPG
                     EAREVDWPYLHAMEERMHRYVPIAEGLGIRKAWAATIEYCPDHLPIVGPVWTRAGDEI
                     PGVTVATAVGHGMMWGPGVARVGADLALAGTTEVTDVSGWGMDRFDEEGRSPSYDPIA
                     LPFPVSTDDG"
     gene            17645..18442
                     /locus_tag="VF195_04190"
     CDS             17645..18442
                     /locus_tag="VF195_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HEX6580050.1"
                     /translation="MRTVTPMQPDTDASPSHPSAPTPVPPAPADRRTLVEEVLVVLSL
                     SVLARAAYAILSLLEAPIAGVVVAAADQSNAFTRQILGFAFGLAPALLVFHLVRRSGE
                     GLPAIGIEADQPNRDLIRGVLVFAVVGVAGIGVYLLAVELGVNRFVVPTPPLGHWWTI
                     PALVMNAVEAGVVEEVIVLGYLITRLQQLSWPAVLAVGASALLRGSYHLYQGWGGFLG
                     NLAMGAVFGYLFLRWGRRTWPFVVAHVLLDVGAGVGFILFRDRLPGF"
     gene            complement(18556..20079)
                     /locus_tag="VF195_04195"
     CDS             complement(18556..20079)
                     /locus_tag="VF195_04195"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HEX6580051.1"
                     /translation="MSTLPRVADPVDASFLRRLNRPWVAILAVTAIAALVRLWGLSNP
                     PSLVFDENYYAKAACIFVGGTDRYCKVNSENEQLFREQEWDVGSYVHPPLGKWTIALG
                     IKAFGMDPFGWRIGSAVAGTLAVAGLALIAQLLFGRPLWTFLAGLLLALEHLSVVLSR
                     LALLDVHVQLWTVAGFLCLILDRRWIDVRTPRGPPEGEVRVPSPLWRPWRYAAGIAFG
                     AAVAVKWSGGLGIVAAIVISLVWETSRRHRSGTTHRGAFARALFRESFSLLVAFVILP
                     FLVYVAIYLPWLHHFDWKVGELIEQQRRVATYHLSGLKETALDPDTGAMTPTHYGYSR
                     PWTWLIMKRPLSITWEDVGPSIRQLTAIGNPVVFWGSIWALPYLAWAWWRKRDWVPGF
                     LVMAVLLQYLPWFLASRPQFFFYVAPFTPFLVLGMVYMVRDLSDARLVVREPGGAVAT
                     DPATGRPAVSVWHPYRPIAWTVILAAVGLFVWFWPVLSWGVISDTRYELILWFPGWR"
     gene            complement(20100..20522)
                     /locus_tag="VF195_04200"
     CDS             complement(20100..20522)
                     /locus_tag="VF195_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HEX6580052.1"
                     /translation="MAEPHKDYSGTPLPQKLGVREGAKVYVAAAPQGFDIALGILPSG
                     VQRLRRPGSRMDVALLFVTEERDLRSRFATLAAGLDPAGRLWIAWPKKAAKLASDLDF
                     DTVQRIGLDAGLVDNKSASITETFQGLQFVHRLKDRPR"
     gene            20578..21402
                     /gene="rsmI"
                     /locus_tag="VF195_04205"
     CDS             20578..21402
                     /gene="rsmI"
                     /locus_tag="VF195_04205"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018762946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX6580053.1"
                     /translation="MSGILYLVGTPIGNLGDLSDRARETLATVTVVAAEDTRRTGRLL
                     RSLDIRTPLVSMFEGNEGERAAELVERLDVGDDVAVVTDGGMPSVSDPGYRVVRRAVE
                     RGIEVRAVPGPSAAIAALVLSGLPTDRFAFEGFLPRRPGDRGRRLQALRHDPRTIVFF
                     ESPRRVRALLREIGAALGDRRVAVCRELTKLHEEVIRGTVAEVLATIGERELKGEVTV
                     VLEGDLEGAPLDRAALLDEVRRLTAEGMRPRDAARSVAERHGASANELYRAAIASD"
     gene            21482..22324
                     /locus_tag="VF195_04210"
     CDS             21482..22324
                     /locus_tag="VF195_04210"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEX6580054.1"
                     /translation="MPPRPDVGGSGAETASSRLLAAFIATSLVAVILGVLAVGAVLRE
                     PSPVEMGAGGPTAAPSATTDSPSSSASPAARAKVDGAFKFLELVGGAPVRWNPCETIS
                     YAVNSDGATSSIGPDLHEALARVTRATQIEFVSVGTTEETFMRAYQRMRYKGVIRKAE
                     LIIIWVDHADYQAILRRLHDPRPSIAFAKTMAGLFADRDQYFGGIIVMDAEATSQRGF
                     GHSYAHGSVLLHELGHIMGLDHVKDPDQLMYSGRYPNYGLHDFGAGDLEGLRRIGIDA
                     GCLG"
     gene            22411..23958
                     /gene="metG"
                     /locus_tag="VF195_04215"
     CDS             22411..23958
                     /gene="metG"
                     /locus_tag="VF195_04215"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015772224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX6580055.1"
                     /translation="MGRDVFTITTPIYYPNDVPHVGHAYDAVAADVIARHHRLRGERV
                     FHLTGTDEHGLKLQRAAEAAGMDPQRWVDEMEPRWREVWARLDIGYDDYIRTTEPRHE
                     AVVVKLLDQVHENGRDDIYLDSYEGLYCVSCELYYVEGDLLEGQLCPIHERPVEVMKE
                     QNYFFRLSAYGDRLLEHYERTPSAVEPDIRRNEVLSLIRGGLRDFSISRTNFDWGIPL
                     PWDPAHVTYVWFDALTNYLTAAGYGSDEETFAQRWPANIHMIGKDILRQHAIYWPAML
                     MAAGIEPPIQVWAHGFLTVGGKKMSKTNATGIHPFELIDRFGVDSYRWYFLREIQFGQ
                     DGSFSLESMVDRHNAELSNGIGNLASRVLAMLRSYFDGVVPEPTFVGAESDLPDVVAT
                     AVRHYDEEMLAVRPTAALAAVYDVVVRANRYMVERSPWTLAKDPARRDELGSILYASA
                     ETLRSLAILLSSVMPSAAGRMWEQLGIEETLEAQRLDHAAAWGRVAPGTRTTKGASLF
                     PRVDTAE"
BASE COUNT         3667 a         8320 c         8550 g         3683 t
ORIGIN      
        1 gaagtggaac gccatgtggc agcggtctcc gttcccgaag tacgcgacgg cgtcctccgg
       61 ccactggttg gcctccgcga gcagcatccg atcctcgaac gaggcgtcga cgtggcgccg
      121 caggtccgac aggaactgca tggtctccgg gaggttctcg ccgttcgtgc cctcccgctc
      181 gaacaggtag ggaacggcgt ccagccgcag cccgtccacg ccgagctcca gccagaagtc
      241 gacgacatcg aacatcgccg cgcgcacccg cgactcatcg aagttgagat ccggctggtg
      301 ggagtagaac cgatgccagt agtactgacc ggccacttgg tcccacgccc agttcgacga
      361 ctcgaagtcg ctgaagatca cgcgcgcgtc ggggtacccc tccggcgtgt cggcccagac
      421 gtagaagttg cgttgccagg agtgcggctt cgccgagcgg gcccgctgga accacgggtg
      481 ctcgtccgac gtgtggttca tcacgacctc ggtgatcacc cgcaggtcgc gtcggtgcgc
      541 ctctttgagg aacgtccgga agtcgcgcat cgtgccgtag gcgggatgga tcgccttgta
      601 cgcggagatg tcgtagccgt cgtccttgag cggcgacggg tagaacggca ggagccacac
      661 ggcggtcgcg cccaggtccc ggatgtggtc gaggcgttgc acgagtccgg ccaggtcgcc
      721 gacaccgtcg gcgttgctgt cctggtaggc gcgcacgtgg agctcgtaga tcacggcgtc
      781 ctggtaccag cgcgacggcg cgccgaggga ggcgagatcc ttcgcagttc cgatcgtgcg
      841 atccgtggcc gacatggacg cggaggtctc gagcgccgcg ggccccgcgg tcgttgggac
      901 gcgaggcgtc acggcgtccg gccctcgccg ggttcgatcg cgaggacgtg accaggctga
      961 tgcccgggat ggagctcgac gaagttccgg gcaccggtcc acgagaacgt ttcccccccg
     1021 aggaggtcac gcgcgctgaa gggcgcgtcc cacgccatcc ccagctccgg catccgcagg
     1081 tccacccagc cggatcgcgg atgcacggga tcgaggttca cgacgacgac cacggggtcc
     1141 ttcccgtcgg ggagcgtctt gctgtaggcg agcagctggt cgccgtccac cggatggaac
     1201 cggagcgttc ggtcgtgccg cagggccgga tgctcgcggc ggatcaggtt gacgcgggcg
     1261 atcagctcgc gcaagccacc ggcggcaggg gttcggggtc ggatctcgta cttctcggag
     1321 tggaggtatt cctcggagcc ggcctccctg ggctcccgct ccatgtgctc gaacgcgggt
     1381 ccgtagatcc cgtagttcgc gccgagcgtc gccgccagca ggtagcggag ccggaacgcg
     1441 ggaggcccac cctcctgcaa ctgatccgtg aggatgtccg gcgtgttcgg ccacaggttc
     1501 ggccggagga agtctctgac tggtccctgc gtgagctcga tcaggtactg ggtcaactcg
     1561 tccttcgtcc gtcgccacgc gaagtacgtg tacgactgat cgaagccgat cttggccaga
     1621 tgctccatca cccggggacg cgtgaacgcc tcagacagga acaggacgtc gggatgatcc
     1681 cgcttcacct cggcgagcag ccactcccag aacgcgaagg gcttcgtgtg cggattgtcc
     1741 actcggaaga tcgggatgcc tcgatcgacc cagaacagca cgacatccag gagcgcgcgc
     1801 cagagccctt cccgatcctt cgtctcgaaa tcgatcgggt agatgtcctc gtaggtcttc
     1861 ggcgggttct ccgcgaaacg gatcgtgccg tcggcgcgat ggcggaacca ctcgggatgc
     1921 tgcgtgaccc atgggtggtc gggcgagcac tggaacgcga ggtcgagggc gagctccatc
     1981 tcgtggcgct tcagctccgc gagcaagtgt tcgagatcgt cgaaggttcc gagctccgga
     2041 tggatcgcgg tgtgcccgcc ggcctccgat ccgatcgccc acggactccc cggatcctcg
     2101 ggtccggccg ccgcggtgtt gttcgggccc ttccggctgg tgcgcccgat cgggtggatc
     2161 ggtggcaggt acagcacgtc gaacccgagc tcagcgatgt cgggcacgac ggcggcgacg
     2221 tcgcggagcg tgccgtgccg agcgggatcg ggagaggccg agcgcgggaa gagctcgtac
     2281 caggcgccgt ggcgtgcccg ttcgcgggcg gcgaccatct cgagctccgg tgagacggtc
     2341 gtcccgcggc gtcgatcggg atgcgaggac atcagcgcat ggaggtcgtc ggacagagcg
     2401 agcgtcgttc ccgcggccgt gtccttgcga cgcatgatcg acgcggcctg tttgagccga
     2461 tccgcgtcgg cgtcggctcc ttcggcgcgg gcgcgccgca ccgcaccgct cacgagcgcc
     2521 gctccttccc tgagctcgac ggccacatcc tggccggcgg cgcggcgctt ctcgaggtcg
     2581 gatcgccacg tcgcccaggt atcgacccag ccctcgatgc ggaagcggta cgtgccctgc
     2641 tccatgatcg cgaggtcgga ccgccagtga tcgttcccga ggggaaccat cggtgcctcg
     2701 ccccagtcgg catcgtcgtg gcggatcagg acacgcgcgg ccggcaggtc gtgcccgtcg
     2761 gtgaacacgt ccgcctcgat cggcacgacg tccccgaccg aggtcttcgc ggcaaagagc
     2821 ccgccgtcga cggcgggcga cacgtgctcg atcacgatcc tcgtcagcgt ggcggactcg
     2881 actcctctct ccacgtatac aggttcggag ccgagacggc cgcgatggga ggatgggtgg
     2941 tgtgaagcgg ttcgcggtgg cgtcgatcgg cctttccctc gtgctcgcgg cagcatgctc
     3001 ccagaccctc gccggcgacc ccaccgagcg gcctccggag cctttcatcg acgagcgtgc
     3061 gagtgagagt cccgctcccg gcgtgagcga cgttgccggg ggtgaggaga cggccgatcc
     3121 ggcgacggag cgaggccgcc cgcgtttcga gggaggcgtc ttccggatcg ggcccacact
     3181 ccgggacgtg ctgatcggga ggaactggac cccgggctgc ccggtggcga tccacgacct
     3241 ccgagtggtc cacgtgagct actggaactt ccgcggcggc gtcggggtcg gtccgctcgt
     3301 gctccacgag cgcgtggcga gggacgtgct ctgggtcttc cagcgcctgt tccgcgcgaa
     3361 gttccggatc caggacatcg agctcgcggc gaaggaccgg ccgatcacgc gggaggacta
     3421 ctggaacaag acacgtcgga gcgtgacggc gtcgttcaat tgccggcccg cgaccgacag
     3481 caccacgttg tctcagcatt cctacggatg ggcgatcgac atcaacccgt tgcagaaccc
     3541 ctacgtgcgc tcggacggga gcacgctccg tcacatcgca cagccgttca aggatcgatc
     3601 actgcagcac atgggcatga tccacgattc ggacatcgtc gtcggatcgt tcgcagcgat
     3661 cgggtgggag tggggcggcc actggcacac gctgaaggac tacatgcact tctctctcac
     3721 cggccgctga taccccgtct gacctgcggt gctaggtgcg aagggggccc gctgctaaac
     3781 ggatcgctga acgccagcgc ggagccgagc cgatcggccg catcacggtc cgccgacccg
     3841 acgtagtggg cgtaggtcct caggagcaag gccgggctgt gaccatccgc acgcacctgg
     3901 ccgcgttcgg cctagccgcg ctgggccagc tccgcggcat cctcgaggct ccctcgacct
     3961 accgcacggc ggtcctccgg gcgctgatgg tgagggcgac cgcaccgacc tcaccacgtt
     4021 gataagcgat ggccgatgac gacgaggtga tccggctgcg gctgctccgg gcgatccggg
     4081 acctttggca catagtcggc tcaggcgaca ttcgccagaa ccatggttca agaggcggta
     4141 cgctcgcccg atgagcgagg aggtccggat ccgaccggtg gcgtcggacg acctgccgat
     4201 cttcttcgag tacgagtccg atccggaggc ggcgaagatg gccgtgttcg agtctcgcga
     4261 ccgagcttcg ttcgacgagc actgggcgaa gaccatgcgc gacgaacgga acatcgtccg
     4321 cgcgatcgag gccgccggag tcgtggtcgg caacatcggg agctgggagc acgacggcga
     4381 gcggtacgtc ggctactgga tcggacgcga gcactggggc cggggcatgg cgacggccgc
     4441 cctcgcggcc ctcgtcgcgg agctcaccga ccgaccgttg ttcgcgcacg tcgccgacac
     4501 gaacgcggcc tcgatccggg tgctcgagaa gtccggcttc cgccggatcg gggagccgac
     4561 cgccgacgcc gacgccgacg gcgtcgttga gctccactat cggctggacg aggccggcta
     4621 gagagcgccg cggcgagcag gcgccggtac tcctggacgg tcgtgtcgtc gccgaccgcc
     4681 gcggacctcg ccacgttgat cgcgatggcc gatgacgacc aggtgatccg gctgcggctg
     4741 ctccgcgcga tccgggacgc cggcgtctga acccggctgc tatacggttg ccgaagcgct
     4801 gctcagacga cccgtcgccc acggtgtcct cgtgtatacc tgagtgccat gtacctgcaa
     4861 cgatgcaagg agtgatacac ggggacacgc atgaaccccc aggagcggga acatgcactt
     4921 ctcgctcacc ggcagatgag cggaaggaac ttccgcgctc ctcctccggt cttgctgata
     4981 cggaccgaag gaggaggggt cgatgcgagg acacacgatc cgtcgagcga tgctgatggt
     5041 cctcggggtg atgctgttcg tgagtgcctc gccccatgtt gcacttggca gcggtggtgg
     5101 cgggtgcgga cgcgccgtga cggacgccga tggcacgagc gtgcgcatcc gcaacttctg
     5161 cttcggcccg acggtcctgc gtgtcccgga gggcgagacg gtgacgtggg tgaacagaga
     5221 cgacttcccg cacgtcgtcc tcggggccaa cgccgcctgg ggtggctacg gcaaggtgcg
     5281 aggcgggggc gaggtcggct accggttcgt gaactcgggc gtctacccct acgtctgcac
     5341 gtaccatccc ggcatgatcg gggccgtcgt cgtcgggaac ggcaagccgg acggcggcgc
     5401 gtacacggtc accacgaacg ccggaccggt gatccaggcg gagtcatcgc aggacgcgat
     5461 ggagccgcag gcggtgccgg tggcagtgac cccgacagcg tgggcgccgg ccgcatgggt
     5521 gatcggcctg ctggtcgtgg ttgcggccgc gatcgcggcc tcccgcaggc gccgggcgca
     5581 cggaaccgtc acggactgag gttcgtacca tcctgaagtg actccttctt ggcgcttcag
     5641 gtccacgctc gcgatcgcgg cgatgctgtt gatcggtgcc tgcgggaacg cgggagcgcc
     5701 gtcggacacg acgggtcctt cccccagtgc gccatcctcg accgcgggtc cgtcttcacc
     5761 gtcaccgaac ctcgccgatg tgcggatccg cctcgagcag gtggcgacgc tcgagcagcc
     5821 gctcgcgatg gcggtccggg aggacgactc cgcgctctac gtggcgcaga agacgggcac
     5881 ggtcgtggcg atccgcgcag cgcggaccgt cgacaaggtg cccgtcctcg atctctccga
     5941 cgaggtgtcg ctcggcggcg agcaggggct cctcgggttg gccttcgcgc cgggaggccg
     6001 cgagctctac gtgaactaca cggacacgaa cggcgacacc cacgtcacgg ggttcgcgat
     6061 gcgaggcgat cgggcggatc cggccacgcg gcgcgacatc ctcgtggtgg atcagccgta
     6121 ctcgaaccac aacggcggca acctcgtgtt cggtcccgac ggctacctct acgtcggtct
     6181 cggtgacgga gggagcgggg gcgatccgct cggaaacgga cagtccctct ccacgctgct
     6241 cggaaagatg ctccggatcg acccgcagct ctcgggtgga gctcccttcc gcatcccggc
     6301 ggacaacccg ttcgtcggcc gcggggacgc gaggccggcg atctgggcgt atgggctccg
     6361 caacccgtgg cggtattcct tcgatcgagt gaccgggggc ctctggatcg gggacgtcgg
     6421 ccagaccgat tgggaggaga tcgacgtcca gcccgccggg tcggcgggtg gtgagaacta
     6481 cgggtggaac atcctcgaag ggagccatcc gttcttcggc gaggagctcc cggacggggt
     6541 ggctccgacc tacgagtact cgcacgccga gggcgggtgc accgtgatcg gcgggtacgt
     6601 ctaccggggt gaggcgatcc cggcgttgta cggcgcgtac ctcttcgggg acctgtgtat
     6661 cggtgagatc gaggcgctcg gcgtcgagaa cggccgggtg agggaacacc gcgtcctcgg
     6721 gcctgtcgtg gagaacctga gctcgttcgg cgaggacgcg gacggtgacc tctacgccat
     6781 gtcgctcagc ggcggcgtct atctgatcac cgccgactcc tgatcccggc cggagccacc
     6841 tggcggccgc ctcgcgtgta cgctccggcc cgatgtccgg agacatccgt ccctcgcaga
     6901 aagccgtctc gttcacggag tccgtgatcc gcgagatgac ccgcctggcg atgctgcacg
     6961 acgccatcaa cctgtcgcag gggttccccg acttcgcggc gccggaggag gtcaaggagg
     7021 ccgcgcggca ggcgatcgcc gacgacgtga accagtaccc gatcacgtgg ggcgccgagg
     7081 ggttccggcg ggagctcgcc cggaactatc gggagcgcta cgggatggac tgggtcgacc
     7141 cggagcgcga catcacagtc acgtgcgggt cgacggaggc gatgatcgcg gcgatgctcg
     7201 gcgtcctcga ccccggcgac gaggtggtcg tcttcgagcc gttctacgag aactacggcc
     7261 cggacacgat catctcgggc gccgtgcccc gcacggtcac cctgcactcg cccgactgga
     7321 cgttcgacga ggccgagctc gcgggggcgt tcaacgagcg cacccggggg atcgtgatca
     7381 acacgcccaa caaccccacg ggcaaggtgt tcacccggga ggagctcgac gtgatcggcg
     7441 ggctctgcca gcgatggggc gtggtggtgt tcacggacga gatctacgag cacatcctgt
     7501 acgacggcgc cgtgcacgtg ccgcccggcg ccgtaccggg actcgaggac cggacggtga
     7561 cgatcagcgc gttgtcgaag acgtactccg tcacgggctg gcgcgtgggg tgggtggtgg
     7621 cgccggcgcc gctctcccgg ggcatccgtt ccgtccacga cttccttact gtcgcggcgc
     7681 cggcgccgct ccaggaggcg ggtgaggtcg cactcggcct ccccgaggag tactaccgac
     7741 ggacggccga cgagtaccgt gagcgacggg acctgtggat cgagacgctc cgcgagacgg
     7801 ggttcaaggt cgagccgccg gcgggtgcct actacgtgat ggcggagatc tcgggcttcg
     7861 ggttcccgga cgacgtccgg ttcgcccggc acatggtcga ggacgtcggc gtcgcggtgg
     7921 tgccgggctc gtccttcttc tcgcgcccgg agctcggcgc gcacctggtc cgcttcgcgt
     7981 tctgcaagaa gctcgagacg ctggaagtgg cgggcgaacg cttgcgcggg ctgcatccgc
     8041 gcgtggcgta gcgccgccgg gagctcctcc cccctcgaaa gcctcagtga tcctacgtac
     8101 gtatcccgat ccgcagggtt cgttcccgag agcgagcgga aggctcgcca cgcgtcgagg
     8161 ggatggatcg cacggagatt gggacgggcc ccccattccg gtcacgaggg aggggtccat
     8221 cactccacct ggagccgatc gtggtcctcg cgcaccgcgc gccgtagcga cgggggaacc
     8281 ttcggcgccg gctcgatcgg atcgatcccg tacgccggca ggatcgatgc gtacgtaccg
     8341 cgggacgacc ggaagacggg ggattccgct gccccgatct agaggccgcc ccagaagcgg
     8401 ccgggggcca ggatgccgga ggggtcgaac gcggccttga gccgacgatg gacctcgagc
     8461 gcggcgaccg gcgcgtcgcc gaggccgccg gggccgcgga tcacgggcgc gatccctccg
     8521 gctcccgcga cgcgcgtccg gagcgacgcg aggcgatcgc cgtccgggag cccgacccag
     8581 gcgatcccga tgcccatcag ggcgcgccag tcgtccgccc cgccgaggac ggcgtcgagc
     8641 cgggaggggg cgacggcgac ctccgccacg atcggcgccg ccaccgcttc gggcccgtcg
     8701 accgtcgttc cctcgtggat ctcgacgtca ccggcagtcc cgcggatcgt cgccgtctgc
     8761 tcctcgatct cctcgggcca tccctccagc gcgatctcga tccgatccga cgtcgcaacc
     8821 acggaggacg ggagggggac ggcgtcgagc agtgtccggc cggcgagcag accgcccgcc
     8881 gccgacacca cgagccgccg cgctttcggg agcggccgca ccttcaccgc gacctgcacg
     8941 atcgcgccca acgtgccgag ggagcctgtc gcgagccggt ggaggtcgta gccctgcacg
     9001 ctcttgacgg tccgcgcgcc gctccgcacg aggcgaccgt cccccgtgac gatctccagc
     9061 tccacgacgc tgtcgcggag tggacccatc cggagccggc ggggtgagga cgcggccacc
     9121 gcgatcgtgc cgccgaccgt ggcgtcgtcg ggcgcatccg acggccattc ctgtccccct
     9181 tgagcgagca cctggcggag ggcgccgacg ctcaggcccg actccacgac ggcgagcatc
     9241 tcctcggggt catgcgagat cacccgatcg agcccggcca tcgcgagctc ctcatcgacc
     9301 tcacaccggt tccccttgtc ggcgtgcgtc cgcccaccca cgatcgccac ccgcgctcgt
     9361 cgctcgctcg cggaccggag cgcgccgacg acatcctcct tcgtggaggg ggtgatcaga
     9421 tccacgagcc ctccgggagg ttcctcgccg catcgccgac cccggcggcg gagccggctt
     9481 gggtggcggc ttggatctgg gccgccacga cgaagtctcc gcaccgcgcg ccgtcgggca
     9541 gcaccttctg ggggttcatc cggcccgagg gatcgaacgc gctccgcacg cacgcctggg
     9601 ccgtgaggtc gtcctcggtg aacacgagcg gcatgaagtc ccgcttctcg aggccgatcc
     9661 cgtgctcgcc ggagagcgaa ccaccggaag ccacgcagag gcgcacgatc tcctccgagg
     9721 tcctcagcac gcgctcgagc actcccggct cgctcctgtc gaaagagagc agcggatgca
     9781 ggttcccgtc gccggcgtgg aacacgttcg tgaccgtgat ggcctcgcga cggcagatcg
     9841 tgtagacgcc gctgaggacc tcgacgagct tcgtgcgcgg tacgacgcag tcgtggaggt
     9901 ggtagtgcgg cgcgatctgg gcgatcgcac cgaacgccga cttccgcccc ttccacagga
     9961 gcgcgcgttc ctcgggcgac tccgccaccc ggatcgtccc caccgcgtgg tctcgcgcgg
    10021 cggccccgac ctcccgctct tccgcatcca caccggcgcg gatgccgtcc acctcgacga
    10081 gcagcacggc cgcggcatcc gtcgggtacc cggcgtgggc gaacgattcc acggcgcgga
    10141 cgatcccctg atccatcatc tccaacgccg cgggcaccac gccgcgcgcg atgatgtcgg
    10201 agaccgtcgc cgcgcagtcc tcgaccgtgg cgaagtcgag cagcatcgtc gtgatcgccg
    10261 gcggctccgg cgtcaggcgg acgcaggccg ccgtgacgag tccgaccgtg ccctcggagc
    10321 cgacgacgac cccacggagg tcgtagccgg ccgcctccgg gccctcggag ccgaggcgcg
    10381 tcacggtccc gtccccgagc acgacgtcga gcgccaggac gtgcgcggac gtgacgccgt
    10441 acgccaggca gtgcgggccg ccggcgttcg tccccacgtt gccgccgatc gacgacacct
    10501 gctgcgagga cgggtccggc atgtacgtga atcccgtacc gcggagagcg acgccgagat
    10561 cgaggttgaa caggcccggc tcgacccagg ccagtcggtc ctcgggcctg atctcgagga
    10621 tgctgtgcat cttcgacgtg acgaccacga gggcgtcgcc gatcggtgtg gacgcgcccg
    10681 cgagcccggt gccgctccct cgagggacca ccgcgagatc gtgcatcgcg gccgcgcgga
    10741 cgcaggcggc gacgtcgtcg gtggagcgag ggaagaccac gaggccggcc gcgccttcga
    10801 ccatcgacgc atcgcgtgcg tacaggcgga gcgccaaggg gtccgacagc acctcgtccg
    10861 gaccgagcgc ttcctcgagc tcccgccgcg ccgcgtccag atccgccacc tcgttagggt
    10921 aggcggggat ggcgacctgg gccgagttcg agctcgccga gccggagctg gccgcgttcg
    10981 ggaaggaacg gctcgacggg caggtctgct tccacgcgac gctccgcacg gatggatcgc
    11041 ctcgggtcca tccggtggag ccgtggatcg ccgtcggtct gttgctcgtt cgattccgag
    11101 gccacagccc caaggtcgac gaggtcctcc gcgacggtcg ctacgcgctc cactcgccga
    11161 tggacaaccc agacggcgtg ggtggcgagt tcatggtgcg gggctggatg gagcagatcg
    11221 gtgacacgca tccggccgtg cagcggttcg tccacgacgc gacgtatccg ctcgcgttct
    11281 acgcgatgtc cgtggaggag gcagtgggaa ccacctacga aggcgaggga accgagcccg
    11341 tctaccggcg ctggcgacct cacgccacgc ggccgggaga gccgtcgggt gcggagaagt
    11401 cgtagcccgc agccgcccag gcctccatcc caccctcgac gttggtggcg tcgtagccgc
    11461 gcgcctgcag catcatcgag gcgagctcgc tccggttgcc gctccggcag atgacggcga
    11521 cgggcttcgc gatgtcgagg acgtcggctc cgaccccggc catcacgtcg ttcaggggga
    11581 tgtggagggc gcccgtgacc ctgcccgcgt cccattcgta ctgttcgcga cagtccacca
    11641 gctggatgtc gtcgagcgac tcgtggacac cctcgaccgt gctgagatcc gccatcgaac
    11701 cgatgctagc agtgggtccg gcgaacggtt cagccggtca tcgcacgttg gaacgccgca
    11761 tgcgcggtcg cgtcgaacag cacgaagcgc acctcgtgca ccgacgtgtc ggccgttcgc
    11821 accgcctcca ccgcgacgcg cgccgcctcg tcgatcgggt agccgtagac cccggtcgag
    11881 atcgccggga aggcgacccg ggcggcctcg agctcgtcgg cgacgcggag cgactccgta
    11941 tggcagctcg acaagagcgt cacgggatcc tcctcgcgcg cgtagaccgg accgaccgtg
    12001 tggatcaccc atcgggccgg gagatcgccg ccggtcgtcg ccaccgcacg accggtgggg
    12061 agaccgtccg gccactcggt cgagcggatc cgccggcatg cctcgaggat cgaggggccg
    12121 ccgcggcgat ggatcgcgcc gtcgacgccg ccgccgccga gcagcgtcgc gttcgccgcg
    12181 ttcacgatgg cgtcgacccg ctgctccgtg atgtcgccct cgaccagaac gatgcggggc
    12241 atgcgtctat cgtaggtcga tgatcgtctg cctcgccgcg aacccgtccg tcgatcggct
    12301 cttcgaggtc gaacgattgg tcaagggcga catccaccgg ccgtccgggt tcgtgcaggt
    12361 cgcgggcggg aaagggttga acgtcgcacg ggccgcgcgg tcgctcggtg ccgacgtgtg
    12421 tgcggtcgcc ctgctccggg gacacacggg tcggtggctg caggaatcgc tcgccgccga
    12481 gggtgtgaaa ggtgagttcg tgtggacgca cggggagagc cgatcctcgc tgtcggtcgc
    12541 cgaccgcgag accgccggac tgaccgagtt ctacgagcac ggcgccgtcg cgccgaacgc
    12601 ggcctggatc gagctcacgc acgccgccgc gatgcgatgg gaacgcggca gctggctcac
    12661 gatctcgggc tccttgccgc ggggagcgcc cgacgagggc taccgggacc tgatcgagga
    12721 agcgcgccgc ggcggcgtcc gcgtcgcgtt ggattccgag ggggagcgtc tgcgcctcgc
    12781 cctcaccgcg gagcccgaca tcgtgaaggt gaacgtcgcg gaggcgggag agctcctcgg
    12841 cgtgccgacg gcgcgccggg acgactccct cgcagcggcc tccaagatcc gcaacatggc
    12901 gggtggcgac ggacacgccg gcatcgtgac gcggggagcg gagggcgtga tgctggcagc
    12961 gccggacggc acgctctacg aaggcgtcct ctacgtgcgt ggccgctacc ccgtggggag
    13021 cggtgacgcg ttcctcgccg gactgatcac cgcgctcgag cgggggaacg actgggagga
    13081 cgcgctccgt gtggcgctcg gggcgggagc ggccaacgcc gagctcgcgg gcgccgcccg
    13141 gatcgatccg tcgcgcgcgg cggccctcgc gaggcaggca gacgtccatc tcctctagcc
    13201 cggtccgggt cgagccgttc gtcgagcacg actgggtcgc gttccgcgag atcaggctcc
    13261 gttcgttgct cgacagtccc gacgcgttcg gctcgaccta cggtgaggaa tcctcgctct
    13321 ccgaacccgc gtggcgagat cgggccgggg ggcgatggcg gggcgggacc gcggtcgtgt
    13381 tggcgggtcg agatcagacg ggcgcgctcg tgggaaccgc gacgggcgcg gagttcgagg
    13441 ccgagctcgg tgtggcgcac ctgtacgcga tgtgggtggc gcccgacgcc cgcggaacgg
    13501 gcgtcgggcg cgcgctcgtc gaggcggtcg tcgcctgggc ccgcggccgc ggatgcggcc
    13561 gcctcgtgct ctccgtcacc gagacgaacc gaggcgcccg cggcttctac gaagcgtgcg
    13621 ggttcgcgga caccggtgag cggcgaccgc tccgcgaggg ctccgacctc gacgtgctga
    13681 tcttcgcgaa gaccttctag gaccgacatg cgtgacgtcg acagggacgt ccgggggcgt
    13741 ggctacactc cgccgcatga agagaccgac gggcgcggac atctcgcccg cggccgccgt
    13801 cctcggcctg ttcctcacgt tcggactgat cgtcgcgtcg ttcttcccgt tcctctcgat
    13861 ctacttcgcc ggacgaggct tcgacccgag ccagatcgga ttcctcctcg gagcgatggc
    13921 ggccgtgcgg atcgtcgcca tgccgatctg gggacatgtc gccgacacgc ggatcgggcg
    13981 tcggcgggcg ctgcagctcg gactcctggg gatggtcgcg ttcgcgatcg tggcgtcggt
    14041 gctcgactcg ctcgtcgggg tcgcgttcgg ggccgtcggg atcgcgctgg ccatggccgc
    14101 gtcagggccg aacatcgacg cgctcgcgtt ggtccagctc ggcgacgagc ggatggccga
    14161 ctacggccgc atccgtggat gggagagcct ctcgtacgcc gcggggtgcc tcgcgttcgg
    14221 ttggttgctc gacctctacg gcgtgtccct cgccctcccg ctgtacgcgg gcgcgggcgc
    14281 ggtgatgctc gtctggacca cgatcgcggt gcgaccggac cgtccgacgc agcccgcgca
    14341 gcacggccgg ctgggagcgg tcggcgcggt cttccggacg gcgccgcgga tgtggggctt
    14401 cctggtcgcg acgctgctcg tgtggacggg attcaacggc gcctggaact tcgtcggcct
    14461 gaagatcgag gagccgctgc tgatcggcgt gggcaccgca ctgggtgggc tgacggaggt
    14521 gggggtcatg cggctctcct cgcgcctgca tcgccgtctg ggcttgcgca aggtgtacgc
    14581 cctcggatgc ctggtctacg cgacggggtt cctgctgtgg ggtctcgtcg agagccgcac
    14641 gatcgtgtcg ctgctcgccg tcttcgaggg cgcagggttc tcgctgctct tcaccacctc
    14701 gatcctcgtc gtcggcaagc tgctgccctc gacgctctac tcgacgggca actcgatcgg
    14761 cgcgatggtc ggcttcggga tcggtccgat catcggagcg gggctgggcg gcgtcgtcta
    14821 cgggcggctc ggctccacga cgctcttcat cggctccgcg gtcctcgccc tcctcggggg
    14881 cgtcgcggcg atgatcgcgc tccggatccc ccagctcgat acccctctga gggagccgct
    14941 tccgaccggg gacgccgtcg ctcccgagcc cggccggctc tgacgtacct gacctgcgcg
    15001 ctttgcagcg aagcactccg tcgtgacacg atgagcgttc cgcagcccca ccggccgatc
    15061 cgacggccgg accccaaccg ggaggtgtga ccgatgacga acaacccgaa cggttccgac
    15121 gaacagccga tcgccggcct cgtggacaaa gcgaaggagg tcgcggagac cgtcgccgag
    15181 aaggcaggtc ccgcgatgga gaaggccaag gaagtcgcgg ggacagtcgc cgagaaggcc
    15241 ggtccggtgg tggacaaggc gaaggaagtg gcggggacga tggtcgagaa ggccggcccg
    15301 gcgatggaga aggccaagga gtccgcaacg gagctcgtcg acaaggcgaa ggagctcctc
    15361 gcgaccaagg acgacgacgc gaaatgagga ggatccgcca aggggggagc aactgccgaa
    15421 gggggagcaa ttgatggtca gcgcctacat cctcatccag accgaggtcg ggaaagctgc
    15481 gcaggtgacg cagcaggtca gtggcatcga cggcgtcgtc gccgtcgacc cggtgaccgg
    15541 cccctacgac gtcgtcgccc gtgcggaggc ggacgacctc gacggactgg ccaagggcac
    15601 ggtcatgccg atgcaggagc tcgacggcat cacccgcacc ctcacctgcc cggtcatcca
    15661 gctctgacac gcgggcctcg gcctgcggaa cgatgacgat cacgatccgg cgcgacgcag
    15721 agatctccga cgtggacggc gggtggttcc gggcgcgctg gcacttctcg ttcgacacct
    15781 accacgaccc ggcgtacatg cggttcggca cgctcagggt gttcaacgac gaccgcctgg
    15841 tgcccggcgc gatctggccg atgcacccgc acgaggacat cgagggcctc acctacgtgg
    15901 tcgagggctc cttccgtcac caggacgatg tcggcggcgc gcccggaccg ctgcccgccg
    15961 ggtccgtgca gcggatgacg ctcggtaggg gcgcgtggca ctcggagcag aacgcgagtg
    16021 agaccgagcc gatgcggttc atccagatgt ggatcatgcc cgcggaacgc ggcttggagc
    16081 ccggcgtcga gcagaaggtc ttcaccgagg aggatcgcac cgaccggctc ctgagggtga
    16141 tcagcggcga cggcggcgac gccgtgctcg tccatcagga cgcgcacgtc ttcgtctcca
    16201 ggctgcgatc ggacagcgac gcgacacatg ccctcggcga gggccgaggc gtctacctgt
    16261 acctgatcga gggcgacgcc gacgtcgccg gggagcggat gacgaccggc gacgcggccg
    16321 agatcgagga cgagccttcg atcccgatcc acgcgaacgc ggtgaccgag ctgatcgccg
    16381 tcgacgttgc gctcgactga tcagccgtcg tcggtactga cggggaaggg gagggcgatc
    16441 ggatcgtagg agggcgaacg gccctcctcg tcgaagcgat ccatgcccca gccggagacg
    16501 tcggtgacct ccgtcgtgcc cgcgagcgcg aggtcggccc cgaccctcgc cacgcccggt
    16561 ccccacatca tcccgtgccc gacggcggtg gcgaccgtga cgccggggat ctcgtcgccg
    16621 gctcgtgtcc agaccggccc cacgatcggg aggtggtccg ggcagtactc gatcgtcgcc
    16681 gcccacgcct tgcggatccc gagaccttcc gcgatcggga cgtatcggtg catccgctct
    16741 tccatcgcgt gcaggtacgg ccagtccacc tcgcgcgctt cgcccggcgc ctcgtcagga
    16801 ttggacatcc cgaacaggag accgccctcc tcgagtcgcc agtacaggcc gatcgcgttc
    16861 tcgaacacca tcggctgccg ccccacgtcg aaggcttcgt ggggctccgt cacagccacc
    16921 tgatgccgga ccgcgccgac gaagaagcgg gcgttcacga gcgcgccgac ctcgcggagc
    16981 ttcggccccc cggtgaggag gacccgctcc gtcgcgatcg tgccctcggg cgtctcgacc
    17041 cctgtcaccg ccccgtccga gcccgtgcgg atcccgagga acgatgtccg ttcccggagc
    17101 tctacggcgg ctcgctgcat cgcgatcgag tacgcgcgca cgttcctcgg agggtccacg
    17161 cacccgtcgc ccggcgcgta cgtcccgccg cggaacgtcg cggggtcgag cgtggggttc
    17221 aggcgcgcgg ccccggcggc gtcgacgtac cggacgtcgc cgaggccggc cccgcgctgc
    17281 atctccacgc gggcattcgc ctcgcgctcc tgcgcctcgt ccgtcgcgag gatgaggtag
    17341 ccgagctcac ggaagccgct gtctgtcccg taccgctctc gctgcgaccg gtagaagtcg
    17401 atcgaccaca tcccgagggc caccgtctcg ggcgtgcccc cttgcgcgcg cacgacgccg
    17461 gccgcgcgcg aggacgcccc ctgcccggcc tcccccttct ccagaacgac gacgcgttcc
    17521 gcgccgtgct ccgtcgcgaa gaccgatgcc catccgccga tcgtgcccgc ccccacgacg
    17581 acgaggtcgg cgctgtctcg gttcggcatc gggtccttcc gctcgaagag gccgaagtct
    17641 agcgatgcgt acagtgaccc cgatgcagcc cgacacggat gcgagtccgt cccatccatc
    17701 ggccccgaca ccggtccctc cggcgcccgc cgaccgccgg acgctcgtcg aagaggtcct
    17761 cgtcgtcctg tcgctctcgg tgctggcacg ggcggcctac gcgatcctct cgctcctcga
    17821 agccccgatc gccggcgtcg tagtcgcggc cgccgaccag tcgaacgcgt tcacccgcca
    17881 gatcctcggg ttcgccttcg gcctcgcgcc ggccctcctc gtgttccatc tcgtccgccg
    17941 aagcggggag ggacttccgg cgatcgggat cgaggccgat cagccgaacc gcgatctgat
    18001 ccgcggcgtc ctcgtcttcg cggtggtcgg ggtcgccggg atcggtgtgt acctgctcgc
    18061 cgtggagctc ggggtgaacc ggttcgtcgt accgaccccg ccactcggtc attggtggac
    18121 gatcccggcg ctcgtcatga acgcggtgga ggccggcgtg gtcgaggagg tgatcgtcct
    18181 cgggtacctc atcacgcggt tgcagcagct gtcgtggccg gcggtgctcg cggtgggcgc
    18241 gagcgcgctc ctccgcgggt cgtaccacct gtaccagggc tggggggggt tcctcgggaa
    18301 tctggcgatg ggggccgtgt tcggatatct gttcctgcga tgggggaggc gcacttggcc
    18361 gttcgtggtc gcccacgtgc tccttgacgt cggcgccggt gttgggttca tcctgttccg
    18421 agaccgctta cccggcttct gatctggccg accctggcgg atcgtgactg gagcaggtat
    18481 actgaggtct cggatatccg aatcaagacc gatcccccgg ccagcgcgcc ggggtttcgt
    18541 gttcctgggg tcctcctacc tccagcccgg gaaccagagg atcagttcgt agcgcgtgtc
    18601 cgagatcacg ccccagctca gcaccggcca gaaccacacg aagaggccca ccgcggccag
    18661 gatcacggtc cacgcgatcg gtcggtacgg gtgccagacg gagaccgcgg gacggcccgt
    18721 ggccggatcc gtcgcgaccg cgccaccggg ctcccggacg acgagcctcg cgtcggagag
    18781 gtcgcgcacc atgtacacca tccccaggac gaggaacggc gtgaacggag cgacgtagaa
    18841 gaagaactgc gggcgcgatg ccaggaacca cgggaggtac tggagcagga ccgccatcac
    18901 gaggaaccct gggacccaat cgcgcttccg ccaccacgcc cacgcgaggt acggcaacgc
    18961 ccagatgctc ccccagaaga cgaccggatt gccgatcgcc gtgagctggc ggatcgacgg
    19021 tccgacgtcc tcccaggtga tcgagagcgg ccgcttcatg atcagccagg tccacggtcg
    19081 cgagtagccg taatgcgtcg gtgtcatcgc gcccgtgtcc ggatcgagcg ccgtctcctt
    19141 caggccggac aggtgatacg tggccacccg cctctgctgt tcgatcagtt cgccgacctt
    19201 ccagtcgaag tgatgcagcc acgggaggta gatcgccacg tacacgagga acggaaggat
    19261 cacgaaggcg acgaggaggg agaagctctc ccggaagagc gcccgcgcga aggctccgcg
    19321 gtgggtcgtg ccgctgcggt gacgcctcga cgtctcccag accagtgaga tcacgatcgc
    19381 tgcgacgatc ccgagtccgc cggaccactt caccgcgacg gccgccccga acgcgatccc
    19441 ggccgcgtag cgccacggcc tccacagggg cgaggggacc cgaacctcgc cctccggtgg
    19501 cccccggggg gtccgaacgt cgatccagcg ccggtccagg atcaggcaga ggaagcccgc
    19561 gacggtccag agctgcacgt ggacgtccaa cagcgccagc cgcgacagca cgacgctcaa
    19621 gtgctcgagg gcgaggagca gccccgcgag gaacgtccag agcggtcgtc cgaacaggag
    19681 ttgcgcgatc agggcgaggc cggccaccgc cagcgttccc gcgacggccg agccgatccg
    19741 ccacccgaag gggtccatac cgaaggcctt gatcccgagc gcgatcgtcc acttgccgag
    19801 cggcggatgg acgtaggagc ccacgtccca ctcctgttcg cggaacagct gctcgttctc
    19861 cgagttgacc ttgcagtacc gatcggtgcc accgacgaag atgcacgccg ccttcgcgta
    19921 gtagttctcg tcgaagacca acgacggggg gttcgacagc ccccacaacc ggaccagcgc
    19981 ggcgatcgcg gtgacggcga ggatcgcgac ccatggacgg ttcagccggc ggaggaacga
    20041 tgcgtcgacg ggatcggcca cgcgtggcag cgtactcacg ggtgccgcgt catcggttct
    20101 cagcgcggcc ggtccttgag gcgatggacg aactgcagcc cctggaaggt ctcggtgatc
    20161 gaggcgctct tgttgtcgac gagcccggcg tcgagcccga tccgctggac ggtgtcgaag
    20221 tcgaggtccg acgcgagctt cgcggccttc ttcggccacg cgatccagag tcgcccggcg
    20281 ggatccaggc cggcggcgag cgtggcgaac cgggatcgga gatcccgctc ctcggtcacg
    20341 aacaggaggg cgacgtccat cctggagccg ggccgtcgga ggcgctggac gccgctcggc
    20401 aggatcccga gggcgatgtc gaagccctgg ggcgcggcgg cgacgtacac cttcgctccc
    20461 tctcggacgc cgagcttctg cggcagcggc gttcccgagt agtctttgtg gggctcggcc
    20521 atcggcccat ggtaggcgga ggcagacgtg ggaccatccc gagatggctg gcccggcatg
    20581 agcggcatcc tctacctcgt ggggacgccg atcgggaacc tcggcgacct ctccgaccgt
    20641 gcccgggaga cgctcgccac ggtgacggtg gtcgcggcgg aggacacgcg gcggaccggt
    20701 cgccttctgc ggtccctcga tatccgcacg cccctcgtct cgatgttcga gggcaacgag
    20761 ggcgagcgcg cggccgaact ggtcgagcgg ctcgatgtgg gggatgacgt cgcggtcgtc
    20821 accgacggcg gcatgccctc ggtctcggac ccggggtatc gcgtcgtccg gcgcgccgtg
    20881 gaacgtggga tcgaggttcg cgcggttccc ggtccgtcgg ccgcgatcgc cgccctcgtc
    20941 ctctcgggac tcccgacgga ccggttcgcg ttcgagggat tcctgccgcg gcgcccaggc
    21001 gatcgcggcc gccgcctcca ggcgctccgg cacgacccgc ggacgatcgt cttcttcgaa
    21061 tccccgcggc gcgtccgcgc gctcctgcgc gagatcggtg cagccttggg cgatcgacgg
    21121 gtcgcggtgt gcagggagct gacgaagctc cacgaagagg tcatccgggg caccgtcgcc
    21181 gaggtcctcg cgacgatcgg ggagcgggag ctgaagggcg aggtcaccgt cgtgctcgag
    21241 ggcgatctcg aaggagctcc gctcgatcgg gcggccctcc tcgacgaggt ccgccgcctg
    21301 accgccgagg ggatgcgccc ccgggacgcc gctcgatccg tggcggaacg ccacggagcg
    21361 agcgccaacg agctgtaccg ggccgcgatc gcttccgact aacggcggat tccctcaggg
    21421 gtggcgccct ccctacactg gccggatggt gcaggcgcct ccccctctcg ggccgcaacc
    21481 ggtgccgccg cgcccggacg tcggcgggtc gggggccgaa acggcgagct cacgtctcct
    21541 cgccgcgttc atcgccacgt cgctggttgc ggtcatcctc ggcgtcctgg cggtcggcgc
    21601 cgtgctgcgg gagccgtcgc ctgtggagat gggcgccggt gggccgacgg cagcgccgtc
    21661 ggcgacgacc gactccccgt cgtccagcgc ctcgccggcg gcccgggcga aggtcgacgg
    21721 tgcgttcaaa ttcctcgagc tcgtcggcgg cgccccggtc cgatggaacc cgtgcgagac
    21781 gatctcgtat gccgtgaact ccgacggcgc cacgtcctcg atcgggcccg acctgcacga
    21841 agcgctggcg cgggtgacgc gcgccaccca gatcgagttc gtgtccgtcg gcacgacgga
    21901 ggagacgttc atgcgcgcct atcagcggat gcgctacaag ggcgtgatcc ggaaggcgga
    21961 gctgatcatc atctgggtcg accacgccga ctaccaggcg atcctgcgcc gcctccacga
    22021 tcctcgcccg tcgatcgcgt tcgccaagac gatggccggg ctcttcgccg accgggacca
    22081 gtacttcggg gggatcatcg tgatggatgc cgaggcgaca tcccagcgcg gcttcggcca
    22141 ttcgtacgcg cacggctcgg tcctgctcca cgagctcggg cacatcatgg gtctggacca
    22201 cgtgaaggac cctgaccagc tgatgtattc ggggcggtac ccgaactacg ggctccatga
    22261 cttcggggcc ggcgatctcg aggggctcag gcgcatcggg atcgatgccg ggtgcctcgg
    22321 gtagcgcctc ggagtcgacc cgtaggaacg gcggcgccac aggaagccac ggcccacagg
    22381 gtgccgacgg cccctgggta cgatgacccc atgggccgcg acgtcttcac catcaccacg
    22441 ccgatctact acccgaacga cgtgccgcac gtcgggcacg cctacgacgc cgtggccgcc
    22501 gacgtcatcg cccggcacca tcgactgcgg ggggaacggg tcttccacct gaccggcacc
    22561 gacgagcacg gcctgaagct gcagcgtgcg gccgaggctg ccgggatgga tccgcaacgg
    22621 tgggtcgacg agatggagcc ccgctggcga gaggtgtggg cgcggttgga catcggctac
    22681 gacgactaca tccggaccac ggagccacgt cacgaggccg tagtggtcaa gttacttgac
    22741 caagttcacg agaacggacg tgacgacatc tacctcgaca gctacgaggg tctgtactgc
    22801 gtgtcctgcg agctctacta cgtggagggg gacctcctgg agggccaact ctgcccgatc
    22861 cacgagcgcc cggtcgaggt gatgaaggag cagaactact tcttccggct ctcggcctac
    22921 ggggaccgcc tcctggagca ctacgagcgg acgccgtccg ccgtggagcc cgacatccgt
    22981 cggaacgagg tgctgtccct gatccggggc gggctccggg acttctcgat cagccgaacg
    23041 aacttcgact gggggatccc gctgccctgg gatcccgccc acgtcacgta cgtctggttc
    23101 gacgctctca cgaactacct cacggcggcg gggtacggct cggacgagga gaccttcgcg
    23161 cagcgctggc cggcgaacat ccacatgatc ggcaaggaca tcctccggca gcacgcgatc
    23221 tactggcccg cgatgctgat ggcggccggc atcgagccgc ccatccaggt ctgggcgcac
    23281 gggttcctga ccgtcggcgg caagaagatg tcgaagacga acgcgaccgg gatccatccg
    23341 ttcgagctga tcgaccggtt cggcgtcgac tcctaccggt ggtacttcct ccgggagatc
    23401 cagttcggcc aggatggttc gttctcgctc gagtcgatgg tggaccgtca caacgccgag
    23461 ctctccaacg ggatcggcaa cctcgcctcg cgcgtgctcg ccatgctgcg ttcgtacttc
    23521 gacggcgtcg tgccggagcc gacgttcgtc ggcgccgagt ccgacctccc ggacgtcgtc
    23581 gctacggccg tccgccacta cgacgaggag atgctggcgg tgcggccgac ggcggcgttg
    23641 gccgccgtct acgacgtggt ggtgcgcgcc aaccggtaca tggtggagcg gtctccgtgg
    23701 accctcgcca aggacccggc gaggagggat gagctcggat cgatcctcta cgcctcggca
    23761 gagaccctgc gctcgctggc gatcctcctt tcctccgtca tgccgagcgc cgcggggcgg
    23821 atgtgggaac agctcggcat cgaggaaacc ctcgaagcac aacgtctcga ccacgcggcg
    23881 gcgtggggac gcgtggctcc gggcacgcgc acgacgaagg gtgcctccct cttcccgagg
    23941 gtcgataccg ccgagtagcg cggcccggcg ctcgatcgag ggcgcgtcaa cgggcgaccg
    24001 acggccacag atgtttcacg tgaatcccct cggatgcacc ggaacttgag accgcctatc
    24061 atcgcgcgcc gatgacccga ccgaaccctg aactcccggt gcagagcggg gtggtggaca
    24121 cccactgcca cctgttcctg gtggaccggg aaccttcact ggtggtcgag gccgcgcggg
    24181 cggcgggtgt cgaccgcctg ctgtgcgtcg gtgtcgaccc
//
LOCUS       DASUYD010000076         2696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_37333, whole genome shotgun sequence.
ACCESSION   DASUYD010000076 DASUYD010000000
VERSION     DASUYD010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37333"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF195_04220"
     CDS             complement(<1..412)
                     /locus_tag="VF195_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6580056.1"
                     /translation="MRIAPGIHSIGDSSIINAYLVEEANEVTIVDAGVSGLYRDLPRE
                     LASMGRSIADVRALVLTHGHSDHIGFAERLRGDRHIPVSVHEADAALARGEVPNPSKG
                     FGPTRLAPLTGFLWFSLLRGGLRTHHLREVSTFGD"
     gene            729..1490
                     /locus_tag="VF195_04225"
     CDS             729..1490
                     /locus_tag="VF195_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580057.1"
                     /translation="MGATPLGRGPATVSLIVIESVGIVRASLRLVFAADPDLMLLGEA
                     EDADAGLELVASLRGQGLRLALVGLELGGDHDAFWLIRSLRERSPELIILATGADLHR
                     GSISQGLFAGADGFVHKNSSPERFVEATRRAAAGEVVLEGLPRGALGGIVEGVESMER
                     QRATTPILTDREKAVLVAAADGLTAREIGRRLGVSERTVTTHLNHIYRKLNVSSRVAA
                     LSAAWRLGLITTPVVSVMARSDEQVSEPRYVAVPG"
     gene            1722..1976
                     /locus_tag="VF195_04230"
     CDS             1722..1976
                     /locus_tag="VF195_04230"
                     /inference="COORDINATES: protein motif:HMM:NF016828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6580058.1"
                     /translation="MKIYMLLQAYTFVRVTGGNFRSRVHDRFVELTHSEVGATAAEYA
                     LLVSLIAIVIVVGAFLLGNAINDRLTATATCIDDATGAPC"
     gene            2081..2473
                     /locus_tag="VF195_04235"
     CDS             2081..2473
                     /locus_tag="VF195_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6580059.1"
                     /translation="MRRGDDRGAVAVEFALLVPLLVVILFAIIAVGIALFRVVNYTSA
                     AREGARYAAVHCRPEATACTPDLIVTRVTQAANGNPISGTPTADRDCSVTPGEPVTVS
                     WEQMVPVHIPLVPDLTFTMTVAGTFRCE"
     gene            2464..>2696
                     /locus_tag="VF195_04240"
     CDS             2464..>2696
                     /locus_tag="VF195_04240"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HEX6580060.1"
                     /translation="MRVGVRLSDDSGATAVIVAILIVVLVGMLALAVDGGILWVKYRG
                     VRTANDAAALAAAYSCATGEGLAGAETAADTIAA"
BASE COUNT          419 a          890 c          923 g          464 t
ORIGIN      
        1 cgtcgccgaa cgtcgagacc tcgcgcaggt gatgtgttcg aaggccaccg cggaggagcg
       61 agaaccacag gaagccggtg agcggggcga gcctggtcgg cccgaacccc ttcgaggggt
      121 tgggcacctc gccacgcgcc agcgccgcat ccgcctcgtg gaccgagacg gggatgtgcc
      181 gatcgccgcg gagccgttcg gcgaatccga tgtggtcgct gtgcccgtgg gtgaggacga
      241 gcgcgcgcac gtcggcgatc gaccgtccca tcgatgcgag ctcgcggggg agatcgcggt
      301 agagcccgga cactccggcg tcgacgatgg tgacctcgtt cgcttcctca acgaggtacg
      361 cgttgatgat gctgctatcc ccgatgctgt ggatgccggg tgcgatccgc atctcgaagc
      421 tccctccgcc gccgaccgtg gaaccaactg ctccatcctg cgccccgtct tgctcagccc
      481 gccagaccgt tgatctccca gaaagccgcg gacaccgccc gtcgaccgtc gtgtgaacct
      541 cgatcctgcg ggcccggtga cccagcccgg cgaggggtcc ggccttccgc gccgggttcg
      601 ccaggcgcct gcgtactgcg ctctacgtaa tacacccgcc cttcggccta ttgcccgaca
      661 tttggcatcc cccgtaggct cgcgaccaat caacaagtgc ggacatcgaa agtctcgacg
      721 gaggacgaat gggcgcgacc cccctaggac gtggtccggc aaccgtctcc ctgatcgtca
      781 tcgagtcggt tgggatcgtt cgggccagcc tgcggctggt cttcgccgcc gaccctgatc
      841 tcatgctcct gggagaagcc gaggatgcgg acgcgggact ggagttggtt gcgtcccttc
      901 gcgggcaggg cctgcgactc gcgctcgtcg ggctcgagct cggcggcgat cacgacgcct
      961 tctggttgat ccgctcgctc cgcgagcggt cgcccgagtt gatcatcctg gcgacaggtg
     1021 cggatctgca ccggggctcg atctctcagg gcttgttcgc cggcgccgac ggattcgtgc
     1081 acaagaactc gtcgccggag cgattcgtcg aggcgaccag gcgggcggct gcgggcgagg
     1141 tcgtgctcga aggtctccct cgcggagcgc tcggcggcat cgtggagggt gtcgagagca
     1201 tggagcgcca acgagccacc acaccgatcc tgactgaccg ggagaaggcg gtcctcgtcg
     1261 ccgccgcgga cggtctcacg gcgcgcgaga tcggccgacg tctcggggtg agcgagcgca
     1321 cggtgacgac ccacctcaac cacatctacc gcaagctgaa cgtgagcagc cgggtcgccg
     1381 cattgtcggc ggcctggcgt ctcggcttga tcaccacgcc ggtcgtcagt gtgatggcac
     1441 gaagcgacga acaggtctcc gagccccggt acgtcgcggt acccgggtag ttcctcgttc
     1501 tgatcgggtc gacgaacagc gacggcgggg cgtccgcata ccgaccttgc tctcagcttg
     1561 atcgcgtaga cggatgttcg tacgcaggtt cctcgaccgc ttagacacta ttcccgatac
     1621 cgcctgcgca gcgaccgtgg ctaccgtcgt cgtcggcaac gtgggatcgc cgggcggcgt
     1681 cgcggacgcg aggtcttccc ggccttcacg tcgggaagga ggtgaagatc tacatgctgc
     1741 ttcaggctta caccttcgtc cgggtgaccg gcgggaactt ccgctcgcga gtgcacgacc
     1801 gattcgtcga gctcacgcac agcgaggtgg gcgccacggc ggccgagtac gcgctgctcg
     1861 tctcgctgat cgcgatcgtg atcgtggtcg gcgccttctt gctcggcaac gcgatcaacg
     1921 accggctcac ggcaacggcg acctgtatcg acgatgccac cggcgctcct tgttgagagt
     1981 cagcctggct tgaagagtgg atccgtccgc tctctcttcc ccgccaagga cggggaagag
     2041 atggaggtcg gagacgttga aaagacgagg agatcgacga gtgagacggg gagacgaccg
     2101 tggtgctgtt gcggttgagt tcgcgctgct cgtgcccctc ctggtcgtga tcctgttcgc
     2161 gatcatcgcc gtcgggatcg cgctcttccg agtggtgaac tacacgagcg cggcccgtga
     2221 gggagcgcgc tacgccgccg tgcactgccg gcctgaggcc accgcttgta cgccggatct
     2281 gatcgtcacg cgggtgacgc aagccgccaa cgggaatccg attagcggga ccccgaccgc
     2341 agaccgagac tgctccgtga cgccgggaga gcccgtcacg gtctcctggg agcagatggt
     2401 tccggtgcac atccccctcg ttccggatct cacgttcacg atgacggtcg cggggacgtt
     2461 ccgatgcgag taggggttcg gctcagcgac gattccggcg caaccgcggt catcgttgcg
     2521 atcctgatcg tcgtcctggt ggggatgctc gccctcgccg tggacggcgg gatcctgtgg
     2581 gtgaagtacc gcggggttcg gacggcgaac gatgccgcgg ccctggcggc ggcgtactcc
     2641 tgcgcgaccg gagaagggct cgccggcgcc gagaccgccg cggacaccat cgccgc
//
LOCUS       DASUYD010000077       103375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_38356, whole genome shotgun sequence.
ACCESSION   DASUYD010000077 DASUYD010000000
VERSION     DASUYD010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 103375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 103375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..103375
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38356"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="VF195_04245"
     CDS             complement(<1..670)
                     /locus_tag="VF195_04245"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6580061.1"
                     /translation="MLELAVLGLLKERPMHGYQLSRELGDSLGGLWRVSYGSLYPTLR
                     RLERDAAIESEAGDVRGARRKKVYRITSKGEQIFLELLQESPQDTQTEDARFRMRLAF
                     FRYLPPETRIRLLERRRQALKERLSTIAESVRTGRGGTDDYGRALIEHNRTITESDIA
                     WLEQLIAAERAKNTPAGGLKRRRAALRGKAGSSSETVARGGTGSSSEAVARGGTGSSS
                     EAVAH"
     gene            complement(752..1915)
                     /locus_tag="VF195_04250"
     CDS             complement(752..1915)
                     /locus_tag="VF195_04250"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580062.1"
                     /translation="MPRTAWILFAGTFVLRLGSFVFPFLALYLTKNGLSVTEAGLAIT
                     GYGIGAMLAQFSGGLLTDRIGRRNAIAISMITSAALVLLLLEARSLPAVLTIVVVYSF
                     FAELHRPASGALIADLIPPEHRVAAYAFNRLLFNLSFAIGLAIGGLLAEEHFRELFIA
                     DAATSVVFGLISLVALPHGTRTSKAQDRAIGSARAAILADRGFLLVLAAILAGGLVYA
                     QGYSTFPLWVRDLGFPERVYGFLQGGNGFLVAIFELGVTAIAMRYPRIRMIALGVLLT
                     GLGFGAFGILLTGIGMAGAVFVWTFGEMFGSPSTAAFIADRAPAHLRGRYQSSLGLTY
                     SLAFTIGPIGGTAIYEWWPRGLWIACAALGVAGAALALAGERYPTPARVELPG"
     gene            complement(1963..2622)
                     /locus_tag="VF195_04255"
     CDS             complement(1963..2622)
                     /locus_tag="VF195_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013152641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6580063.1"
                     /translation="MSAPTKRTQVHRHPERGDYGRATIDAILDEGLICHVAFNDGDGA
                     PRCLPTIHARVGDTIYLHGSTAARAWKALRAGAEVCIAVTIVDGLVMARSAFNHSMNY
                     RSVVVFGTAREVTDEDELHTAAAAITSHVAPGREDEARMPTAAEYRQTLLLAVPIEEA
                     SAKVRTGPPKDDEEDMGLPIWAGVLPLHTRPGEPEPDPQLSGDLDVPPSVSGYHRPGG
                     Q"
     regulatory      2750..2903
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2946..3878
                     /locus_tag="VF195_04260"
     CDS             2946..3878
                     /locus_tag="VF195_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX6580064.1"
                     /translation="MIHRRIRGSAAVLLLLLASCGESTTPQASPETPSPAPAFPMTIT
                     DDDGVEVTIVKEPDRIVTFAPSMTEIVFELGLGDRLVGVSGPFDDYPAEAKDIEEVGG
                     AGDFGVDPTIEKVVSLEPDLFLTIPGGDQWKQRLRDLEIPVVTLHADTFDDLLTDIGT
                     MGVLTGAVDDAGTLAERMRSEADAIGQSAQVVGPVSCFFEVYYPPLTTVGPDTFIYDL
                     LQRAGCDPVTGEATSDYPEWSVDELVADGPQVYLVTPESAKSVAAVSKRPGFAGIPAI
                     EDGRVELVDGDLVTRAGPRVIEGLRELAEALGTA"
     gene            3875..4921
                     /locus_tag="VF195_04265"
     CDS             3875..4921
                     /locus_tag="VF195_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEX6580065.1"
                     /translation="MTRPGTRRFRRTMVVLAVALVVTVLIAVAVGAVWISPGTTLRVL
                     GWKLGLTGHPDGVDRASEVILLQLRLPRVALAVLVGAALGVSGMLFQGLFRNPLADPA
                     VIGVSSGAALGAIVVIVLGGASIAGGLVVSGAAFAGALLTTLLVYGIARVGPTVQVTT
                     LLLAGIAVAATISALMSLVMSFSGEQIRSIYFWLLGGLGARGWDSVSVVVPFVVAGGG
                     ATLLLARDMNLVSLGEERAGQLGVEVPSFKRRSIAAGALLAAAAVSVAGVIGFVGLMT
                     PHLLRLLVGVDHRRLLPAVLLGGPLFVVLADLAARTVLAPEELPLGAVTALVGAPFFL
                     WLLRRERGRAVGVA"
     gene            4932..6179
                     /locus_tag="VF195_04270"
     CDS             4932..6179
                     /locus_tag="VF195_04270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580066.1"
                     /translation="MLDGVTAGYGPSDVLRDVSLVVRSGEVVGLVGPNGCGKTTLVRV
                     ACRALKPATGSVRVTGRDPYEMSSKEAARLVAVVPQDVIPAFPFGVLEFVLMGRAPYL
                     SRWTGGGPADWAKAREAMETVGVQHLADRPMDELSGGERRRAVLAQALAQDAPVLLLD
                     EPTTHLDIRHVVELHQVIRRLADERGVAVLAVLHDLTLAAALSDRLVAMAGGQIVADG
                     QPEEVVTRSLLRTVYGVNADVEASPATGRPAVILGPPEHAIASTGLRALVVGGAGRGG
                     PLMRLLVERGVHVVAGVLHGSDTDVEVAERLDIEHVSVPAFSEVDEEAAMVWLDHAAI
                     ADVVVVCDPPVGPGNVRNLELALDAGIRGMPVVVFDAMPIAERDFTGGRATELWTRLR
                     DLGEAVGSYPDAVTAILSHAPIR"
     gene            complement(6236..7072)
                     /locus_tag="VF195_04275"
     CDS             complement(6236..7072)
                     /locus_tag="VF195_04275"
                     /inference="COORDINATES: protein motif:HMM:NF021287.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043248 - proteasome assembly [Evidence
                     IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAC2 family protein"
                     /protein_id="HEX6580067.1"
                     /translation="MSLYRLEEQIPVFTAPVLIVAFDGWIDAAGAATACANHVADGGR
                     MVASFDVDALHDYRARRPVLDVVDGVLARMQWPEIMVRHVTFDGRDLLVLVGPEPDYK
                     WKQLGDDLLELALRLGVVEWISLGAIPAAVPHTRPVPVLATASTDASLRDGEQRGPGG
                     ILRVPAAALSAVEMTVAAAGIPAVGYYAQVPHYIGAAAYAPATVALLQHVERRLGVTI
                     PVGPLGEEALSQRERLDAAIAEDHDTKEYVARLEAIANEERMPSGDDLATEIERYLKE
                     QS"
     gene            7137..8138
                     /locus_tag="VF195_04280"
     CDS             7137..8138
                     /locus_tag="VF195_04280"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6580068.1"
                     /translation="MDGVRAGRRGPLRFLAVPAGAVLSVALAGCAGDVYGEFPSPPTP
                     AITATPSAEADNGILAEIAVDGSPCFLAEAGDRVWVTAFDGNELAEIDPETNEVVSTQ
                     RMPGGPCGMLEVHGTLWIETPNAGAIVRFDPVRGEVIDRLRVPGGVFGLTSTPSGIWG
                     VAGQGEEVVQIDPGSGEIVGRVEVDGPLGGLVFAREQVWVAAAGALVRIDPRTQTVVD
                     EIEVESFEPEGLAVDGDLLWVSSSFEQSVLRFDLRTMKVRDRFPVDDALFGGIVLGDS
                     YWVSGNNTLYRLDVETGEVEDELELVGFGPIPAAGDLWTVDFIANAVFRLDERAPEE"
     gene            8200..8631
                     /locus_tag="VF195_04285"
     CDS             8200..8631
                     /locus_tag="VF195_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580069.1"
                     /translation="MSAPDQNVYRVHLADPGGRISVLDVPTEHFESMERGISLGTTTI
                     IPWHRVIRYTREVVQAVDDAFLTRAEIRAWLDDGSDEGERIVVRADRFDAGTSTADFL
                     VERTLDIEGGVVHLTKIHVPWGRVLEYERLPVRVEVPSRPD"
     gene            complement(8642..9058)
                     /locus_tag="VF195_04290"
     CDS             complement(8642..9058)
                     /locus_tag="VF195_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003265786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6580070.1"
                     /translation="MSDDRYERGRERFLEVHDEKALAAVEGLGDLGRAIVEFAYGDVY
                     TRRGLALRDREIAAVAALVATGRSSQIPQHLRASLNAGLTPEELREIILQTATIAGFP
                     PAMNAMSTLKSVLKDVGAKEAAEAGEEADEKEAREE"
     gene            complement(9110..10309)
                     /locus_tag="VF195_04295"
     CDS             complement(9110..10309)
                     /locus_tag="VF195_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020540834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6580071.1"
                     /translation="MRFAPTDVKFIADPYRDYDELRRSAPVVYDEATDHWLISRYADV
                     DALLRDRRFGRTYLHTSTHEEMRRPAPPDWHAPFWDLINAGILDMELPDHTRVRRLVA
                     KAFTPRFVESLRPRVHAIFEDLLKGLAGAGEFDLLPTLAEPLPVIVIAQMLGVPEADH
                     HLLRPWSADIVKMYELNPSEPAQHDAVRASLEFSGYLRALARERRTAPGDDLISGLVH
                     VIDEGDTLTEDELVGTCVLLLNAGHEATVNATLLGWWALFRHPDRLKELRADPSLLPT
                     AIEELLRFQTPLPMFERWVLEPFELHGVEIPRGAELGLLFDSANRDPEVFDEADELRL
                     DRKPNPHLTFGAGIHFCLGAPLGRMELQISFGRMLERFPNLELVEEPTFRPNYIIRGL
                     DGLRVHA"
     gene            10483..10761
                     /locus_tag="VF195_04300"
     CDS             10483..10761
                     /locus_tag="VF195_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor"
                     /protein_id="HEX6580072.1"
                     /translation="MASRKQVSAAKRNIKSAQKAARKKRTISKLPKSVRQDLGRQAAA
                     ARRRGGAPGRGLEDRTREQLYEQAKTEGISGRSRMGKWDLIEALRRSR"
     gene            complement(10796..11026)
                     /locus_tag="VF195_04305"
     CDS             complement(10796..11026)
                     /locus_tag="VF195_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580073.1"
                     /translation="MTAAGSDRDPMDVGQPEEAQMADDDLAERKPSPPGAPDEEKAED
                     ELEPLEGGAETLADLDEEAGQTNRPGTERPPV"
     gene            complement(11376..11831)
                     /locus_tag="VF195_04310"
     CDS             complement(11376..11831)
                     /locus_tag="VF195_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580074.1"
                     /translation="MEPRRIDLDALLTAHAFMRVERPPELWQVDVDDESFIRMLGEMI
                     VVGLLHHDVLAELTLNASNVTVGEEGPDGVVPGDYVAVTIHGPGVWERDMAWTPASAE
                     PLWSTDLDVAVRAASVSFGYLRSSSQEEGSITVFIPRSLPTEGSSNPSG"
     gene            complement(11837..12673)
                     /locus_tag="VF195_04315"
     CDS             complement(11837..12673)
                     /locus_tag="VF195_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007262123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6580075.1"
                     /translation="MTTMIGADADALRPTPFPQIAFHRRLFRTPSKGEGGGSVIVGPH
                     IGTSGWQYDDWRGVFYPDDVPQRDWLAYYAERFGTVEVNNSFYRLPQRATFERWRDQT
                     PPGFVVTVKASRFITHLKRLRDPEEPVELLWERAAGLGDRLGPFLFQLPPRFPVEIGR
                     LRKLLQVLPKGMRPAFEFRDRSWYTDDVFLLLDDAGAALVWPDGPGMRHTLPMTAGWL
                     YVRFHQGTVHGPGYRRDKLRRWASRISATGAEETYLYFNNDPTGAAIRDARTMIELLS
                     ER"
     gene            12672..12947
                     /locus_tag="VF195_04320"
     CDS             12672..12947
                     /locus_tag="VF195_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580076.1"
                     /translation="MRGDRVEIVVDIGNGVQTYEIEATRAGRRVEVTTGRGVVEVSEV
                     TRGGKAVRTGRFMTARVVALVEHPAEGEPAVGPRRTRRAVRGQSSLL"
     gene            complement(12954..15761)
                     /locus_tag="VF195_04325"
     CDS             complement(12954..15761)
                     /locus_tag="VF195_04325"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580077.1"
                     /translation="MRCEFCGHPLPEGARFCPNCGTPVSTTLGTEERKMVTVVFADLV
                     DSTGLAQRLDPERARDVLGGFFDAASEELRALRGHPEKFIGDAVMAVFGLPHVQEDDA
                     VRAVRAGLAIRGRMHRLQQSLGLEEPLEIRVGVETGEAATGVGPTGQLLVTGQVVNAA
                     ARLQAAAQPGEVLVGATTHALTKDAVSFGPQQEVEAKGFAGALQAYQVDQLTTRSARR
                     TIPFVGRANEMDILRGSVARVKATASPLLVSIMGEPGVGKSRLADELLAGLDSDVTVV
                     RGRAQTYGDTATFAPVTAIVREIAGIEDDMAPEEAMRRLQDVVDGCCDPSEAERVAGR
                     LGLTIGLGGPKREESVFVQDVQSGFLALVEGLSARGEVVLVFEDVHLLRPPMLDLIER
                     AASATKTAPKRVLTIALGRPELLEERPAWGSGAVNAVTLRLDPLPPLEAVELVREAAG
                     GRVADAEAEEIASRTGGNPFFIVETTGMVLRARQDGAASSGRELPPTVQAVVAARLDS
                     LRHRLRDLARRASVFIFSFDREELATVSDAEPEDLQGLEEAEILVRDDAGRATRWRFR
                     HETVREVAYGSLPKRERRNLHEKIAQALMESGHRSYAADHLERAALASLDLDPADRRL
                     PELAADALVLAGDRARRRMESRSALDYYARALVMAGPEDAWGVREGRVLAGTGEAHYW
                     LGEYPAASEAMARAVELGETFDDAWTLALALRYQGDIAINVDGDLDQAERLLADSLAA
                     AGELDDAWAMSRSLLFAGWVPWTREDFDQADSIWHRALELAEEHDDTWARVRALTALS
                     INESQKNQTEEAARLIEQARVLAEEMGDRFSVAVTMVQRGRLDEDLGNHAEAIPRFDR
                     AIEIFKELGARWEMADALAERGISYRDLGRLDEGEEDLKSAIRISGELGEQQIAGWSG
                     RALDRLAQIRAENADERVSGG"
     gene            15823..16395
                     /locus_tag="VF195_04330"
     CDS             15823..16395
                     /locus_tag="VF195_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6580078.1"
                     /translation="MHITTFERPEDFRRWLERHHSSERELWVGYYKKSSGRASMTWPE
                     SVDEALCFGWIDGVRKRIDDERYAIRFTPRRAKSIWSAVNVGRVAVLAAEGRMRPAGR
                     KAFEARREDRSGIYSHERRDEAVLSPPFERRFRATKRAWAWFEAQPKGYRQNTIRWVM
                     SAKRQATRERRFATLMEDSAAGRRIAQLRR"
     gene            complement(16442..19777)
                     /locus_tag="VF195_04335"
     CDS             complement(16442..19777)
                     /locus_tag="VF195_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6580079.1"
                     /translation="MTIVTMGRGIVRLRRSLALVMGLVLVLTLQGVGHSAPQGKRPTL
                     RSEGAGLPDGFLPAAVRARQLGRYFVEMKVPSAASVALRSRGTAGASTSVPTQDAARA
                     ALRSQEGAIGQARSLGGQVIFRYGVLINAFSARLSAQAAGQLAQRTDVASVQPVGIVR
                     KHLTTSVPFIKAPEVWSTYGARGEGMRVAVVDTGIDYTHASFGGAGTVAAYEANDPTF
                     IETGTFPTEKVVGGFDFVGENYDVLDEETSNDTPRPDFDPLDEDGHGTHTASTVAGVR
                     VPGQVGRGVAPMAKLYAYKVWDVGNSTDDVLVAAYERAVDPNQDGNVADAVDVLSFSG
                     GVSYGTLNSLEAKAAQRVVDLGTVFVASAGNEGHQAVGGSPYILGTPASARGVVAVAA
                     SIDEFVALQLVINTTSDGTIPELPDNGLTVHQDWSADLPSGGITDDLFDGREIDPAES
                     PADAMFCDPLPGGSLTGQTVLVLKGSTGAGDCDGTTKVFNAQEAGATAVILVSQFGGL
                     PFGLGPGEHVDEITIPAVMISGADGEAILLELSPNAPATYNDGAVNATISDVPAIIPG
                     FDDAFTDFSSEGPARLTSDLKPDISAPGFNISAAAVGTGNEAIELSGTSMAAPHVSGV
                     ATLLRQLHPSWNPAQIKAVMMNQANRNMKNNDLTTPVSATLMGSGRVDAFQSARARSV
                     AWPGSLSFGFAPTPTKWSAIRSFRVRNFDSGSHGYSVTGVDRYSDFDPAMTSLKVSTD
                     GSTFGGSRSFTLGAGRSQRVWVRLTVNPNVVSEPEQEFGWYYFHPNMDGTVQVTQSGA
                     PADTLRLPWHVAPLATSDNGLSETFLDLTTDPATMELTEGPAAGISYADLYLLGATDP
                     AGSRGEEDLVAVGARSFTGSTIDGTAEGVPTGTDALGGFTWQEFLTNADEPTEPVEFG
                     VQAWGVHNTTETLEVDVFVDAGADGVFADADLQADYLVAKLPGPGGEVCVFDLSLPAP
                     FDECAALYFADYSNFNSNLVGLVVDASAIGLDDNAPELAYRVEACTGTFSGDVPGLIC
                     DSVGEIDEGTGTWNLTLNAIDPALVIDPLVCKGFWDGGDCDSVSPITVTTGSAGADDD
                     PSILALFPNDAPHRTPTVVETDT"
     gene            19935..20522
                     /locus_tag="VF195_04340"
     CDS             19935..20522
                     /locus_tag="VF195_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6580080.1"
                     /translation="MPPQRTSAGRSTHRVAGLPAMRALAHPTRVRMLHLLRAEALSAS
                     ELARRLGIRVGSAQYHLRSLERAGIALRVGERRRRGGTEILFEVPHGLWVDVEPDAPP
                     GMRQAMNRAYVAELLRRMDAAAIERHDVDRDVLSTREIVLDAGGIDAASEALHVFLHR
                     LDELALPAPTEGSTTFTIATLFLQMPKEQTPRDDT"
     gene            20519..21739
                     /locus_tag="VF195_04345"
     CDS             20519..21739
                     /locus_tag="VF195_04345"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580081.1"
                     /translation="MSDDVTNPIRDAPAFRRLLAARTISHIGDGVALVALVLMVQEER
                     RTGTAVGALLLATSLPRFLGPIAGAIVDRVEQRTLMVACDIGQAATFGVIAWLEPSFP
                     VLVGLVAVAAAFDTLFSPAGRSALPALVRPEQLMRANAWIGMSLNIRVAVGPILGGLL
                     VEWLDFRGALAANALSFVLSALFLLGLPPLRAPSEGTEARGFLTVGMDGLRFAWRNRV
                     VRTLIVALFVGVAFAGLDDVALVFLVRKTLEGSPLAYGLVTGAYGVGMLLGSLGLSWK
                     GTAAAAGTVFLTGWVAGGVGAVLTGIAPLIALVAVGQAIAGVGNAVEVVAMDTLVQQA
                     VPRQMLGRVFGLVGTAAPAGHTLAFAGGGFLVDLFGPRIVFLIAGLGVLLVLIPVMLV
                     LRRADATGTEGPSA"
     gene            complement(21731..22975)
                     /locus_tag="VF195_04350"
     CDS             complement(21731..22975)
                     /locus_tag="VF195_04350"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580082.1"
                     /translation="MPRTFSAQELSDEAGVSQHRVEWLSDVGILRPEGPGSFVWADVF
                     RVKMASALLDAGLSEAMLSRSVAEGWLNMEFAGEYLPYEPGPRSARTFGAFVADSGAA
                     SGRLPAVFETMGQPPPDPDAPIHVDEEDMLRRFLDVWGAAPGDDTMIRAARLLADGVR
                     IATLGWVELVDEQFGEPARERLASREIDRFPDEVRRKYTTAANLVPELFTWLSARYLE
                     RRSVEGIVEGFERFLALKDLTPMPEPLAPPAVAFVDLSGFTELTEARGDRIAVRSVVA
                     LQEQAHAVAMEHGGRLVKLLGDGALLRLPDAERGLSAALGIVAAMDEEGTTRAHAGVH
                     AGPVIERDLDLFGRTVNLASRIADAASPGEVLTSETVVEALAGGRFRFEPFAQTELKG
                     IAEPTALYRVLRDGSGGRGRSG"
     gene            23047..24399
                     /locus_tag="VF195_04355"
     CDS             23047..24399
                     /locus_tag="VF195_04355"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6580083.1"
                     /translation="MAALTSFSQAFEGEIILPGSAGYDAARIVWNGMIDRRPAVVARA
                     AGVEDVRRTVRFAREQELPIAVRCGGHSLAGFSTCDDGVVLDLSLMRDVHIDPARRTA
                     RVAGGTLLSQLDAAAQAHGLVCPVGVVGHTGVAGLTLGGGMGRLQRHFGFSIDNLRSV
                     ELVTADDRVLTVSENEESELFWGIRGAGANFGVVTSFEFDLHELAPVVTQGFVDFPAE
                     RGHEIAARVREYLETAPDEVMVSFGVSTGTEEDGADLVGRPLVYVGATHSGSPDDAED
                     DLRPLRDLGPVADTFRLVPYLELQTSGDEDMAWGKRFYMKGAFLAELSDAFVDAAVEH
                     ATTPHGAVTLWAQGGAILRVSEDAMAFTGRDAAFWIGVEAEWDDAAEDETLIDWGRTT
                     MAAFEPFTAAGHYVNDVIETGGDVVRSVYGDTKYERLVGVKRAFDPDNVFRLNQNIEP
                     "
     gene            24396..24905
                     /locus_tag="VF195_04360"
     CDS             24396..24905
                     /locus_tag="VF195_04360"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003950682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6580084.1"
                     /translation="MTPIRLTGPRVTLRSTTPEDATPLTPIRNEPEVLRWWGHLEPGE
                     MDEFVADEHSLVIEVDGLVVGAVQYGEEEDPMYRNANIDVYLTASRHGEGLGSEAVLV
                     LARYLIGERGHHRLTIDPAADNAPAIRSYEKIGFRPVGVMRSYERGPDGTWHDGLLMD
                     LLADELPPD"
     gene            complement(24914..25387)
                     /locus_tag="VF195_04365"
     CDS             complement(24914..25387)
                     /locus_tag="VF195_04365"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6580085.1"
                     /translation="MDHQLDLAAVARLMIDSNMYMVLGTADGSGRPWVSPLYFAADGY
                     TEFFWVSSPEATHSRNLTLRPEVSVVIFDPQVPIGTGQCVYMAAFAEEVSEGEVDRGI
                     DTFSRMSVVHGGRAWARQDVLAPAPYRLYRATASEHWALDPTSHPDRRTRVTLGR"
     gene            complement(25412..26803)
                     /locus_tag="VF195_04370"
     CDS             complement(25412..26803)
                     /locus_tag="VF195_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6580086.1"
                     /translation="MRSTNGHDPELVLRLGKDVRGRVIAPDDPEYDEARAVFYGGFDR
                     RPAAIVRVANDADVARVIAEARSTGLELAVKSGGHSIVGHSVAEGGIVLDLSEMQALE
                     IDPESRTAWAESGVTAGTYSEAAAEHGLATGFGDSPSVGIGGITLGGGVGFLVRKHGL
                     TIDALLAADVVTAEGEVLRVDERSHPDLFWAIRGGGGNFGVATRFRFHLFEVPSIVGG
                     ILILPATPETIAGWATASQAAPEELSTIATVMKAPPMPFLPEAAHGKVVILGMIVHIG
                     GGEAGERAVAPFRELAEPLADMLKEMPYPGIYMEEQSDFHPIASDRTMFLDRVDQDVA
                     ATMLERIRTSDAMMAAAQFRVLGGEMARVPADATAFAHRTSKIMVNVAALFEQMEDRP
                     RYEEWVQAFADVLRQDDTGAYVNFLEDEGEERVRAAYPGATWDRLREIKRRYDPANLF
                     RLNQNIPPANEAS"
     gene            26928..27533
                     /locus_tag="VF195_04375"
     CDS             26928..27533
                     /locus_tag="VF195_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6580087.1"
                     /translation="MLDLEVIDNPEAAGVTLDPVRSRLLSELAEPGSATVLAGRLGLP
                     RQNVNYHLRALEDHGLVELVEERKKGNMTERVLRATALSYVISPAALNGVEPDPARFP
                     DRLSARWMIALASKLVRDVGELVAGANKARKRLATFAMDGEIRFASAADRTAFAEELA
                     ASVTSLVSKYHDESAPRGRDHRVIVAVHPSLKPDTTNEEEI"
     gene            27535..28287
                     /locus_tag="VF195_04380"
     CDS             27535..28287
                     /locus_tag="VF195_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014133914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6580088.1"
                     /translation="MAHPFELTHEFEVDASPEEVWDAIATGPGVDAWFMGTNEIEPHE
                     GGATRMAFRGSSEDGVVTTWQPPTRFAYRTPEGEDGALHAFEYLVEGRDKGSTVVRWV
                     HSGFLGDNWEKEYEGLSEGDPMYFDKLRIYLTYFKGRTATPVNVFGGTVVPDRDEAWG
                     KFHSALGLDRAPALGDRVRLTPEGLPMLDGEVDWLSRDFLGVRTDDGIYRFMHISVFG
                     GPTGVGHHIFVDGLDQAETERAWETWLSGVFA"
     gene            28347..29120
                     /locus_tag="VF195_04385"
     CDS             28347..29120
                     /locus_tag="VF195_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007909345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580089.1"
                     /translation="METVRSKDGTTLAFDRLGSGPPVVLVCGGSVDRMADAAIAQELA
                     SDFTVLNYDRRGRGDSGDTLPYAIEREIEDVEAVIEAAGGSANLWGSSSGAALAMIAT
                     ASGAPVEKLALWEPPYFLDPDARPPADQVEQYETMIAEGRRGDAAAYFMGKVVGMPPE
                     FVADARNQPWWAGQEAIAHTLAYDARIMGDYMIPTETAARVKVPTVVLAGGADMPFMR
                     ETAQTLADALPNGEVRFLDGQGHNVDPTVLASALKEFFE"
     gene            29145..29594
                     /locus_tag="VF195_04390"
     CDS             29145..29594
                     /locus_tag="VF195_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003329837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HEX6580090.1"
                     /translation="MRFRTTLEQAGKTATGFEVPSTVVEGLGAGKRPAVRVTINGFTY
                     RNTIASMGGRFMVGVSAENRAGAKVAGGDVIDVDIELDTAPREVTLPKDFAAALKKDA
                     NAKKTFDALSNSNKGWHVSSIEGAKTAETRQRRIAKSIAALHEGRSR"
     gene            29623..30402
                     /locus_tag="VF195_04395"
     CDS             29623..30402
                     /locus_tag="VF195_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580091.1"
                     /translation="MADGETPEPVPDRDEERARIAALLEAHAPPDWEQRFQREQLLDA
                     IIDTTPLQMFVMAADIRESTLLMKEAVQFERFAKIMDKFVSAVRSGIGTPGGWFDKFT
                     GDGFLAYWIAPSTPKEEYQRRFVEAAGNLAHTSHELIELFHRRVLEDFRRNSRNLSDG
                     VGLAIGLDAGPGFLVEIAGELTLVGPPVVGAVRMVNAASRPQEIFANVYLGEHLHDEQ
                     DGIYEALGMTATRESRPTKDYPKGQEVYALTFRDAEGKPAG"
     gene            30419..31678
                     /locus_tag="VF195_04400"
     CDS             30419..31678
                     /locus_tag="VF195_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019931763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HEX6580092.1"
                     /translation="MERHPRLLPVAEEARVTPIELFFDLVFVFSLTQVTALMGAAPTG
                     RGVARGMLVLSLLWWCWVGYAWLGNVVQAEEGLGKATMFASMAAMFVMALSVPEAFDD
                     RDGGLPGPMVIAFAYLAVRVLHLAIFWMVAGTSEDRGLRRQLIRFTPSLAGGTAILLV
                     ASQLTGTAQTLAWVAALLVDYVGTILGGASGWRLRSASHFAERHGLIVIVALGESIVA
                     IGVGVSDVAISWPIIAASVLGLVVAGCLWWAYFDVVSIVAERVLRRLEGEERTRLARD
                     AYSYLHLPMVAGIALVARGIEQVLHQVGHSSDGELSRSLAIVPLASLYGGVALFLFAH
                     VAFKYRTWGRVTLHRLGVAVLICASIPLATELPALAALAALSAVLVAMIATEAIRYAE
                     AREQVRHEEGGPEVHRAHREADQGSAG"
     gene            complement(31755..32201)
                     /locus_tag="VF195_04405"
     CDS             complement(31755..32201)
                     /locus_tag="VF195_04405"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6580093.1"
                     /translation="MKTGISTSTSAAIDAPVDEVWRALTTPEVIKQWFFGVDTETDWK
                     QGSPIVHTGEWQGKPYVDKGEIVRIEPPHVLVHTHWSDLSGTPDSPEHYQEVTWEVSE
                     RNEGSDGSELTITEHNLPSDDAKDASDQAWSMVLDNLKQLLEGDRW"
     gene            complement(32238..32717)
                     /locus_tag="VF195_04410"
     CDS             complement(32238..32717)
                     /locus_tag="VF195_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019738620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX6580094.1"
                     /translation="MPKLSAGLLAYRRTDAGLDAGLEVLLVHPGGPLWAKKDEGAWSV
                     PKGEYEPSEDPLEVARREFEEEIGQAPPDGSEPVWLGELRQPSGKVVSAWAMEGDLEV
                     GEVRSNSFEMEWPPRSGRTQEFPEVDRAGWFGLEEARRKILRGQVGFLDRLEELLAS"
     gene            complement(32728..34269)
                     /locus_tag="VF195_04415"
     CDS             complement(32728..34269)
                     /locus_tag="VF195_04415"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020577636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6580095.1"
                     /translation="MLLGEVAAASADVAATSSRLEKVARLSSVLEALEPGEVPIAVAY
                     LSGVLPQGSIGVGWASLKDLPSPSPSPATLELLDVDSALARIGALAGAGSQGARRTAL
                     HDLFARATDDEQDFLRGLLMGEIRQGALEGVMVEAIARAANVPRTEVRRAAMLTGDLG
                     ATATAAIDGGSAALAHFGLSLLTPLQPMLAQSAEDIPAALARISPAAVEWKLDGARIQ
                     VHRLGDEVRAFTRNLADVTDRVPEIVEVIRSLPIEAAVFDAEAIALRPDRRPHPFGLT
                     MSRFGSRSRKDLTEFQTDYPLSAFVFDVLHLDGDDLIDRPAVERFAALDERLPEELRV
                     PRIVTADVEEADGFLEDAIARGHEGVMVKALDAPYEAGRRGTGWIKVKRAHTLDLVVL
                     AAEWGHGRRRGRLSNLHLGARDPETGGFVMLGKTFKGMTDEMLAWQTERFQEIETGRE
                     GITVHVRPEMVVEVAFDGLQPSPRYPGGLALRFARVKGYRPDKRADEADTIETVRAIH
                     ELSVS"
     gene            complement(34311..34676)
                     /locus_tag="VF195_04420"
     CDS             complement(34311..34676)
                     /locus_tag="VF195_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580096.1"
                     /translation="MNFNSVLIGSENPQQLAEYYTKLFGEPTWNEGGYIGWMIGSGAV
                     TIGPHDQVKGKNAEPGRVIWNVETAEVKSEFDRFVAAGATVVQEYSPGEEMDGLIATF
                     ADPDGNYFQLMSPMGPEGS"
     gene            complement(34769..35143)
                     /locus_tag="VF195_04425"
     CDS             complement(34769..35143)
                     /locus_tag="VF195_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580097.1"
                     /translation="MEITGELLQLGSVEEWRAWLEEHHAGEDGVWLTISKKGASTPSV
                     PLVPATEEALCFGWIDSAMHPIDDERYALRYTPRRKGSNWSAVNRARAQRLIEEGRMT
                     HAGLAAIEEAKRNGRWNDAGEG"
     gene            35253..35762
                     /locus_tag="VF195_04430"
     CDS             35253..35762
                     /locus_tag="VF195_04430"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6580098.1"
                     /translation="MSSSLLEDAFGHHVWATLLVLDACLALPPEQLETSVPGTYGSIL
                     DTARHTVGADASYLFVLSGGRVTPIDEDAMDLAELRSTMEGHGAAWSETLAQDLDTDV
                     MLVRHRDDGSETHAPASIRLAQALHHGTDHRSQIATALTTLGVEPPPMDVWDYGNELG
                     RVTEFPPTS"
     gene            35759..36562
                     /locus_tag="VF195_04435"
     CDS             35759..36562
                     /locus_tag="VF195_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX6580099.1"
                     /translation="MTTDRQASKAVAFRALHDGEPFVIPNPWDAGSARVLEALGFRAL
                     ATTSSGLAFTLGRLDGSVTLEEMAAHAGALDRATELPVSADLENGYGADPEQAATAIR
                     RAADAGAVGGSIEDFDRDEHRLYPPDHATARIVAAVEAARELPFPFTLTARAENHIRG
                     NPDARDTIRRLQAFEEAGADVLYAPGLETIDDIRTICGSVGKPVNVLARPAMRLAELV
                     DAGARRVSVGGALTWTAVKAFAAAAAAIRDVGDLSALDADVPLADWFGG"
     gene            36765..37355
                     /locus_tag="VF195_04440"
     CDS             36765..37355
                     /locus_tag="VF195_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580100.1"
                     /translation="MQRIRTAAVVLAAGLVVTACQSDTTGELVDEPRAGLGGMGIVPS
                     GPPTELLGPEFFPCHPGRGDASYTKYGVGRMRRGGLEREELEPDHIVIDGHGVNFHGR
                     DRVLDGGTIEMEMDEDYFGPTVLRGQAGATVTIELENEGVRQHNFSVRGQGIDLTCGV
                     RARDEVEVVFPRSGLLAFTCKFGDRSGMRGAFVVEG"
     gene            37384..38400
                     /locus_tag="VF195_04445"
     CDS             37384..38400
                     /locus_tag="VF195_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012689877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6580101.1"
                     /translation="MEAIVQDRYGSTDVLELREIERPSAGDDEVLVRVHAASVHADVW
                     HAMKGVPYVLRAMGSGLHAPKHHVPGSDLAGVVESVGSNVTRFQPGDEVFGLSISANL
                     WRNGGAFAEYAAVPVTTLEPKPTRLTFEQAAAVPTSGSLAVQHVRDEARIRSGQTILI
                     NGAGGAVGSFAVQLAKAWGATVTAVDATEKLEMLRSIGADQVLDYTKEDFTRRDERYD
                     VVLDIAGNHPWRECRRALAPDGTYVLSGFDQYGRSGHRWFGSLGRFARLIVIAPFVSA
                     LHPFRGAKDPGDRLVVLKELIEAGKVTPFIDRTFPLSEVPEAIRYLETGRARGKIVVT
                     VSPG"
     gene            38457..38909
                     /locus_tag="VF195_04450"
     CDS             38457..38909
                     /locus_tag="VF195_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020158574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6580102.1"
                     /translation="MRELSTQIEFDGTPDEVWEVLQDLAAHAEWNPFITKIDGELRPG
                     AKLDVRLEPEGERGITLHPTVLAVEPGRELRWLGHLLVPRVFDGEHRFLIEEMGLGRV
                     RFTQSERFGGILLPLFWKKLRDGGTAKGFRAMNEALARRVAGLHPQAV"
     gene            38940..39470
                     /locus_tag="VF195_04455"
     CDS             38940..39470
                     /locus_tag="VF195_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003971313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6580103.1"
                     /translation="MAEIVAEARELLEREGPEGLTMRRLAEAMKIRAPSLYKHVSSKE
                     DLEALLMAEAFRGMGAELHGAITGARKGGSKTTALAELGRAYRRWALAHPHLYRLVTN
                     GPLPRELLPEGLEAWTAEPVVVAVSGDPDRARVAWAFAHGMTILELDGRFPPGADLDA
                     AWAAGIDALTEPERKS"
     gene            complement(39502..41112)
                     /locus_tag="VF195_04460"
     CDS             complement(39502..41112)
                     /locus_tag="VF195_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003928312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEX6580104.1"
                     /translation="MDEAFTAEMASGYAFDEPCVILGRPMKDGEVETGALVQLPLSMM
                     NRHGLVAGATGTGKTKTLQIMAGQLSAAGVPVFISDIKGDVTGLSRPGDATAEAIRDR
                     SAKLGIPFEPQAHPVELLSLSGALGAPVRATVSSFGPLLLGKVLDLNKTQVSVLTLVF
                     TYCDDQRLPLLDLTDLRTTLKFLSSDEGAPVLEEYGGFSKATIGVLLRSIVELEAAGA
                     DAFFGEPEFVVDDLLRTTEDGRGVISLLEIADVMDRPRLFSTFMLWMLAQLYATLPEV
                     GDLPRPKLAFFFDEAHLLFDDASDALMDQIELTARLIRSKGVGVYFVTQSPTDVPSSV
                     LSQLGNRVQHALRAFTPDDADNLRKTVKTFPITAHYDVGETLTSLGIGEALVTVLTPK
                     GVPTPLAATRLVPPDSMMDAVDATTRSELIAAGALREKYGTSVDRESAHEILMGKLAG
                     AAETTDAPPLPPPPPAGEGTTRPEGRAGSRSKSKGRPKPRPEGKASQKSMGDQAKDIG
                     IGLAKDLFTTADGRRTLRTVFGTLLGKK"
     gene            complement(41141..41584)
                     /locus_tag="VF195_04465"
     CDS             complement(41141..41584)
                     /locus_tag="VF195_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6580105.1"
                     /translation="MPDARDDLVTDSPTGWVAKHVRRYVESDGKEGHRWSGVDTLLLT
                     TRGRSTGKLRRTALIYGEDRGRYIVVASNGGAKAHPHWYQNLAADPHVFVQVAGDRFG
                     ARARTATARSKPRLWRLMVSIWPEYERYQTRTAREIPVVVLERLD"
     gene            complement(41657..42589)
                     /locus_tag="VF195_04470"
     CDS             complement(41657..42589)
                     /locus_tag="VF195_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEX6580106.1"
                     /translation="MTGNGAGVIRADEEFVELRPLLFSIAYRMLGSVAEAEDVVQDAF
                     LRWRRAAEEGAEVDSAKSYLAATVTRLAIDHLRRAKTRRETYVGPWLPEPLVAGTEDD
                     VAEQAEMADSLSMAFLVLLESLKPVERAVFLLREVFEYDYAEIAEIVGKSEPNTRQLF
                     TRARRRIEAGTPRFVPSGEERDELAQRFFAACQDGDMDGLLELLAADSVAVGDGGGKV
                     YAAREPIHGAQRVATFLVRLFDQGRRIGARLELTRVNGQPGALAYDSEGLLLTVLSLE
                     VVGGRIRAVRSVANPDKLGHLGPISSYGRRDPVE"
     gene            42738..43700
                     /locus_tag="VF195_04475"
     CDS             42738..43700
                     /locus_tag="VF195_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6580107.1"
                     /translation="MKVFVVGATGAIGRQLVPLLVARDHEVVATTRSPEKTNAVRAMG
                     AEPVVVDALDREAVMSAVTRAGPEAVVHQATALTGVFNLKHFSRTFEQTNRLRTLGTD
                     NLVAAARTAGARRFVAQGFAGWPYASDGRALRTEEDPFEPSPPEEMRSTVHALRHLET
                     AVLGTEDLEGIVLRYGGFYGPATSLGDGGAMVEGIRKRQLPIVGDGAGVTSFLHVEDA
                     AAATVAALERGAPGVYNIVDDEPASSSMWIPELAKAVGAKPPRHVPVWLAKPLIGDAG
                     VWLMTKAPGASNAKVKADLEWTPRYASWRIGFREGLRAERALGA"
     gene            complement(43689..44381)
                     /locus_tag="VF195_04480"
     CDS             complement(43689..44381)
                     /locus_tag="VF195_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX6580108.1"
                     /translation="MSTKIEGGAETRAPLTRDRVLSTAVALADQDGIESLSMRKLAQA
                     LDVVPMALYRHVANKDELLNALVDVVVGEIDPPLEGTDWKTALRARILSARRALLRHP
                     WASRLMESRTTPTPVVLGYLDSMIGMFRTGGFSLDLTHHALHAMGSRMFGFTQELFND
                     SSEADPDDEALFGAMADTHPHIFEIYTTISHDDRSVVGAGCDDQFEFEFALDLMLQGL
                     EKLRDAPPISGT"
     gene            44503..45474
                     /locus_tag="VF195_04485"
     CDS             44503..45474
                     /locus_tag="VF195_04485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6580109.1"
                     /translation="MKAIVQDRYGSADVLEFRDIEEPVVGENDVLVRVQAAGCGPDVW
                     HLMTGMPYMARVAIGLREPKLKVLGWDVAGTVEAVGANVKTFRPGDEVMGQAKGSFAE
                     LVTAKADELVPKPSRLSFEQAAALPVSGVTALRAIRDEGNVQPGQSVLVIGAAGGVGS
                     LAVQIAKAQGATVTAVSSGSKAELVRSIGADDVIDYTREDFTDGSRHWDVIVDTAGRR
                     PLSQLRRALTPKGTLVIVGGDGGGKWTGGFFRGMLRARVVSLFVGQRLRGLATKIAQE
                     DLVALAELVEAGTVTPVIDRTYPLIEASDAIRHLEEGHATGKIVITV"
     gene            46043..46933
                     /locus_tag="VF195_04490"
     CDS             46043..46933
                     /locus_tag="VF195_04490"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6580110.1"
                     /translation="MTAELQARVEAALRRAGRRPGLLVLSATDGGDTAFASRGPLPDP
                     PCAPERAVFEIGSITKVFTSLLLAIAAERGELGVDDPLVEHLPRGTRVPMRGGRPITL
                     VDLATHRSGLPRLPPGFLLHALRHRDDPYARLSTEGVLAALEETRPRVPAGERFRYSN
                     YGAGVLGIALAHAAATDYEALVRERITAPLGLRDTVITLSEDHRSRLAAGTKGWGGPA
                     GLWAIPGLTGAGALRSTAADLLTFIRAQMGTLPDVPEELAGAIRSSHLARAAGGRLPR
                     GCALRSDGSWSGSVVRRTVS"
     gene            46930..47088
                     /locus_tag="VF195_04495"
     CDS             46930..47088
                     /locus_tag="VF195_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580111.1"
                     /translation="MMHNGGTGGYRSFAGWAPDGRHGVVVLSANVRSVDGIASRLLLD
                     LEGTSGSP"
     gene            complement(47085..47237)
                     /locus_tag="VF195_04500"
     CDS             complement(47085..47237)
                     /locus_tag="VF195_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580112.1"
                     /translation="MVARWGTWILVVVLAVGAVMNAASSSPWERYLWAPLALVLAVMC
                     FFVGRG"
     gene            47584..48192
                     /locus_tag="VF195_04505"
     CDS             47584..48192
                     /locus_tag="VF195_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015053873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580113.1"
                     /translation="MRKLIVTTFLTFDGVMQAPGGPEEDDSGGFAHGGWSVNYWDDMM
                     GEAKTEEMSGPFDLLLGRRTYEIFAGYWPNAPEEAGGKGFNDATKYVCSRSRPSLEWT
                     PAMLMEGEAAEGVAALREEDDGPELQVHGSGNLAQTLIHHGLVDEYHLWVFPVVLGKG
                     KRLFADGTIPAGLRLVDSKISTTGVFMGTYEPAGEVVTGSFG"
     gene            complement(48226..48474)
                     /locus_tag="VF195_04510"
     CDS             complement(48226..48474)
                     /locus_tag="VF195_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580114.1"
                     /translation="MGPRRHGGGHGHRSGGGHQYSWWIDVERPGRFSGLGNLGQYPYV
                     APDADAVIVRNGSDWNIDNDTWLGTFREIADQLAQSAG"
     gene            complement(48619..48939)
                     /locus_tag="VF195_04515"
     CDS             complement(48619..48939)
                     /locus_tag="VF195_04515"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6580115.1"
                     /translation="MSPELAERDERFGRITLRDLLTMSSGLRYEEPDIPVPWGDDVVT
                     YYGVDLRDGGINGSQIVEPPRVTWHYNNYNPLLFGDGPRASHADVGLAVHGHQVVAAD
                     RRGK"
     gene            48945..49340
                     /locus_tag="VF195_04520"
     CDS             48945..49340
                     /locus_tag="VF195_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580116.1"
                     /translation="MTGALTSPISPSSIAMPTSVDKNDFATENDVCKVARSDPPKYRS
                     YTRRSRWTTTNASVSFRRRNESKSAPAPSPSGIAPVRSISGSSGNGLTSSPAAITRDG
                     NRCWSPTSVSRQTIARAVVDESTAAHAHA"
     gene            49573..51411
                     /locus_tag="VF195_04525"
     CDS             49573..51411
                     /locus_tag="VF195_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6580117.1"
                     /translation="MRDSSDAARNDRPTRDRDGYLPIRGYAAIGDGRTVALVALDGSI
                     DWLALPNLDSPSVFGALLDASRGGRFSLEPALPYETTRRYVPHTNVLETTFTTEVGTV
                     RVTDALLLSDPGLAPLRELVRVVEGIDGMVPMGWSVEPRFGYASARTSIAVRDGVPVA
                     SSGPDALGIASYGLGRPDIADGRITGRAEVRAGSTGTLVLSAASQEPVVVPSRDEVLD
                     RLDRTRDAWSGWTAERPYAGPFRDAVVRSGLALRLLVFSPSGAIAGAATTSLPEQIGG
                     VRNWDYRFSWVRDAAASLEALIALDCPVEAEAYFWWLMNASQLSRPNLRVLYRLDGGT
                     QARERTLDLAGYRRSRPVRAGNAAIDQLQLDIYGDLLHCSWLYADTVGELDRDLGKRL
                     ARIADLVCRIWRTPDSGIWEVRSEPRHYTQSKMMCWVALDRAIALAGSSRVPSDRVHR
                     WTRERDAIEAFVEERCWSERLHSYVRSDGSEDLDASVLHASILGYGEPAGERMVGTLA
                     AIDRHLRDGPLVMRYVVDDGLPGEEGAFLACSFWLVDAWARAGRVDEAERMMERVIAM
                     ANDVGLYAEEVDPGDGSFLGNFPQALPHLALIGAATAIEEARRDRT"
     gene            51426..51884
                     /locus_tag="VF195_04530"
     CDS             51426..51884
                     /locus_tag="VF195_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010693158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580118.1"
                     /translation="MAGGVLGTFVLTTVLRTASELGLTRVDLPFLLGTAVTRDRGRAK
                     AIGYVAHFGFGLLFSLAYAAFFLVVDASGWLLGAVLGLAHGLFAGTALVNVLLPVVHP
                     RMGKPWTDARSSPLIESPGFLMLNYGAGTPVVSLVAHILYGAIVGGLAAW"
     gene            complement(51910..52719)
                     /locus_tag="VF195_04535"
     CDS             complement(51910..52719)
                     /locus_tag="VF195_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580119.1"
                     /translation="MTALDVRLEAVFESDDAPDVPVPDEVASAYGGSFRLAERVVYAN
                     FVSSIDGVAAIGGVAMSSAAISGGASTDRFMMALLRAVADAVVVGAGTLREHDGPWTA
                     EKAFPPGAESFSRLRAGISASESPTFVVVSASGDLPAEHPALSDAIVVTTAAGARSLA
                     EGRARVGEVIDVGDADRVDGAVAIDRLRERGYQRILTEGGPRLMGAMLEASAVDQLFL
                     TISPKLIGGGPERTPLTDETDLLERGMGARLLSIRRSEDFLFLRYGLSPPG"
     gene            complement(52716..53423)
                     /gene="folE"
                     /locus_tag="VF195_04540"
     CDS             complement(52716..53423)
                     /gene="folE"
                     /locus_tag="VF195_04540"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HEX6580120.1"
                     /translation="MRTDREIPDGRHSTARPADVGERWAMSDPEETTSWERVRPREVT
                     DEQWRRFEDAVAEMFTAFGMDLDTEGTHNTPRRFVRAMYEATTGYEGDPKLLTAFPTE
                     CRGGPDCSISQVIEGPIPFFALCEHHAFPFYGVAHVGYVAHEQIIGISKMTRLVRVYA
                     RRFTVQERLGQQIADHLNDVMDPHGVAVHLQAAHLCTQMRGVREERSRTSTSFWRGNY
                     ERDPEMRREFLTVAQRE"
     gene            complement(53529..55613)
                     /locus_tag="VF195_04545"
     CDS             complement(53529..55613)
                     /locus_tag="VF195_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013491758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HEX6580121.1"
                     /translation="MLTRRDFLKVTGGSTVAWYVATQSGWIQRAVASIPGGSLEPGVI
                     PRFVTPLLIPPVMPRAGVITQRGSQPIDSYEISMKQFSQQILPATMPATTVWGYGAVR
                     SASKRGLLVHNAPSLTIEARWKRPVRITWINDLVDANGDFLPHLLPVDPTLHWANPPG
                     GTEGRDMRPTFETTPGPYTGPVPIVTHVHGSASVGDESDGYAEAWFLPDANDIPGDYA
                     TEGTRYDFFANKAASKYGVAWGPGSATFQYPNSQRASTIWYHDHALGMTRLNVYAGPA
                     GFFLIRGGPDGDDAVLDSRSGTPAVLPGPAPKPNDQFPSNKTYFEIPIAIQDRSFDAN
                     GSLFYPDTRAFFDGITGPYIPETDVSPIWNPEFFGNTIMVNGNTWPSQTVERRRYRFR
                     FLNGCQSRFLILDFSEIPGVEVWQIGNEGGFLAAPVNLTEVNGNRLLMGLAERADVVV
                     DFTKVPVGEHVLGNLGPDEPFGGGIPGVDFEPADPASTGQVLQFRVVPAVAPDPTTPP
                     RFMVLPAITRLTGGTTRPLALLEEMSEFDEPPEDFEEAPVEAHLGTIEGDPNIETASM
                     TALGWDDAVTENPQVGDTEVWEFYNATGDAHPMHIHEVLFQVVDRQDVVIDEESGTVR
                     VDGTSEPSPPEPWEDGWKDTVIAYPGQVTRMRMRFTNAGQFVWHCHIVEHEDNEMMRP
                     YRIGPRQPGQPS"
     gene            complement(55843..56208)
                     /locus_tag="VF195_04550"
     CDS             complement(55843..56208)
                     /locus_tag="VF195_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580122.1"
                     /translation="MKLTRKDLIATLLIAAVVIPYVGYLIWGEMPFIQDPRGMAGVGV
                     VGLLLGFVAWGLGLRSAFGKMMLVLGVATLGLGIAAVLVGAEGSELLLATFVGAIVVV
                     WAIETMHDAGLFGTGAHAR"
     gene            complement(56351..57124)
                     /locus_tag="VF195_04555"
     CDS             complement(56351..57124)
                     /locus_tag="VF195_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6580123.1"
                     /translation="MGFEDLMRDRSAARYADFVLPAIRRGDHVLDVGCGPGSITLGIA
                     QAAGHVTGIDVDDAEFADARAYAAGHGIGNVEFREGSIYDLGVAEGSIDVCTLLSMLE
                     TLDDPLAGLAEVRRALKPGGVIGASSIEYGGLILHGADVVSLRRFYELRLALWEAQGD
                     VHPSRGRELRALLLAAGFAQVEASTTYFSYGTEELVRTFGLGRAADCRDEWFVWGLTE
                     RGLADRDEIDALEQAWIRWAEEPDSFAAFAWGRATARKP"
     gene            complement(57137..57847)
                     /locus_tag="VF195_04560"
     CDS             complement(57137..57847)
                     /locus_tag="VF195_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017681577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580124.1"
                     /translation="MTTPSIVLVPGFWLGAWAWDEVAGLLRAEGHDVTALTLPGLESA
                     HVDRSSIGLSHHVDAIAEAVKAAGDPVVLAVHSGTGFSGYVASDRVPGSIAAMVYVDT
                     APGIPPLDADFDGDEKPLSWEELSAEENLDGLSEGQLETFRQRAVPEPGGVLRDTVEL
                     TNDARLDLPSTIICTGYTSQQYKEAMGEGYAWLAGLHEVRDLTWVDLPTSHWPMWSRP
                     KELAEIIGDVAKSHASDH"
     gene            complement(58026..58520)
                     /locus_tag="VF195_04565"
     CDS             complement(58026..58520)
                     /locus_tag="VF195_04565"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6580125.1"
                     /translation="MIRPAYQMWPQYQRRLRDAIATMTGEQLAIQPSPERWPVWATVG
                     HLACQRVFWLCVFAGEAGVETTPFPNSAFDCPGDDDLEHVWSADRLAGALDSTFRIVE
                     RCLDTWTLEMLGEEIRRPEWDASWVYTRGSVIQRVFSHDIWHTAEVNEALGNAGLRRI
                     DLWD"
     gene            complement(58525..59106)
                     /locus_tag="VF195_04570"
     CDS             complement(58525..59106)
                     /locus_tag="VF195_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017591437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580126.1"
                     /translation="MGRLVINNSMTVNGAFEAPVPEPDGWLVLDPDSNQVSLEQFFAA
                     DAMVLGRKTYEGLAAVWPTLADDPNLKGFADQMNSMPKYVASRSLRAPLEWNATLLEG
                     DLAESVTALKERHDRTLVAVGAGELADALITQGLIDEFWFWVSPSIWPTGPRIFDGVG
                     PVRLQLIGATTFGSGVVRLAYRPAPDRHDGAGA"
     gene            complement(59135..59371)
                     /locus_tag="VF195_04575"
     CDS             complement(59135..59371)
                     /locus_tag="VF195_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580127.1"
                     /translation="MDFFPLLFLTPNFVPRPLLTRPMEIAATYNPVTYIMEAMRSLVL
                     QDLEWGAIGRGFAVVAGAGALMVLLSVRSIRRYD"
     gene            59699..60433
                     /locus_tag="VF195_04580"
     CDS             59699..60433
                     /locus_tag="VF195_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006478517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HEX6580128.1"
                     /translation="MTERRVGTREEWQAARDELLRLEKEHTRMGDDLARRRRELPWVP
                     VEREYRFDTGDGTRTLVELFDGRSQLLVYHFMFGPSYGAGCPTCSSSADAVNAVLSHL
                     HARDVTMLYVSRAPIERLLAYEERMGWSFPWVSCGNSDFNFDLGFSYTADQVREFAGP
                     MLEGGAPSGVEANAAASGTDIPGYLTEGPGFSAFIEDDGAVYHTYSTQARGLEFLMGY
                     YPILDRAPKGRDEGEGQPWLRRHDEY"
     gene            60438..61421
                     /locus_tag="VF195_04585"
     CDS             60438..61421
                     /locus_tag="VF195_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006138623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HEX6580129.1"
                     /translation="MTSILRPKALRPGDLVAIVATSGGLEKDEAPLFERGVGVLDQMG
                     FVVRVSPLVDLDRSWWWAAAPPKEVAEEFNRHLRDPDVRAIFSLTGGRMTLSYLDLID
                     VEAVRADPKPLLGMSDISALHLGLHARTGLVALHADLVTFGFGEWNEVDDARRDQLAD
                     LYRCVLTEDTPLGPLPAGTRWERWRSGRAEGPLIGGMLNRLVRVQASSSALAPERFDG
                     AILFWEEAFTSTSVVWNDLHVLRQAGVLDRISGMVVGRPFEVEPTPPGPESLRDIVLE
                     VVGDRDIPVLGDVDIGHAGPNLPIPLGIRAEMDADAVTLSLLEPAVTISGG"
     gene            complement(61460..62020)
                     /locus_tag="VF195_04590"
     CDS             complement(61460..62020)
                     /locus_tag="VF195_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013736439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOPRIM nucleotidyl transferase/hydrolase
                     domain-containing protein"
                     /protein_id="HEX6580130.1"
                     /translation="MTTIPRAVVLVEGMSDRRALEALAARRGRVLAVDGVAVVAMGGA
                     KNIGRFLERFGPRGSDVAVAGLYDVAEAPDVRRGLQRAGFASGSSPLELETFGFYACI
                     EDLEDELIRSVGAERVVDIVGERGELGPFRTLQKQPEWRRRPIDQQLRRFLGNSSRKI
                     DYAPLLVDALDLSRPPRPLDAVLAHV"
     gene            complement(62017..63216)
                     /locus_tag="VF195_04595"
     CDS             complement(62017..63216)
                     /locus_tag="VF195_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate cyclase regulatory domain-containing
                     protein"
                     /protein_id="HEX6580131.1"
                     /translation="MDGISDRDVAERAGVDLDYVTRLVHVGILAPADDGAFSEGDVRR
                     TRLYQGLERTGLPLEAIREALDAGELSFGWLDAPLYDLIAPLSPRSFEDVAADTGIPL
                     ELLRVLREAIGFALPEPDDRMREDELRVVPLIRKLVSAGFPTSILDRLLRVYGDGLRR
                     MAEVEADWWHRYVVLPALASGMTDAEMHEATLGFGEETTPLLEQAMLAMYHAHQEHTW
                     VENLIGEIEDALDRTGLRSRLVRPPAICFLDITGYTRLTEERGDEAAAELAARLTPLV
                     QRPAERHGGKVVKWLGDGVMFHFREPGGGVVAALEMVDAIAAAGLPPAHVGLDTGPVV
                     FQGGDYFGRTVNLAARIGEQAAPGQVLVTQEIVDRVVPDGIAFEPVGAVELKGVSEPV
                     LLHAVTR"
     gene            complement(63272..63486)
                     /locus_tag="VF195_04600"
                     /pseudo
     CDS             complement(63272..63486)
                     /locus_tag="VF195_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226316.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(63595..63777)
                     /locus_tag="VF195_04605"
     CDS             complement(63595..63777)
                     /locus_tag="VF195_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580132.1"
                     /translation="MADAQTLCPKCGGATDGWKCAICGSVAGEHDEHHLHAGSDRYCT
                     MRCAACQQADVLCTCV"
     gene            complement(63858..64358)
                     /locus_tag="VF195_04610"
     CDS             complement(63858..64358)
                     /locus_tag="VF195_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009358745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580133.1"
                     /translation="MATVSVEDAGSASVERDLKWGSWLMGIAAVGFIGYAVIFFIQNF
                     TDAFLELGIGPNQVDVGRDEIQAFSPSLYHYIGHLHIAVAGFIAATGVAVLFLVVYGV
                     RKGLRWAWTGAVAAPVLGLAVALPDHYPNNFDTIGHLGLIYLATAIFVAGALLALRGV
                     NASQRG"
     gene            64490..65083
                     /locus_tag="VF195_04615"
     CDS             64490..65083
                     /locus_tag="VF195_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580134.1"
                     /translation="MSSTGTTDRTKEAFQELVRKELPGLYSLARRLVRDGAEDLVQES
                     LLQGYRAFGGLKEEAAGGRWLRTILVNVFRDQLRKRARSIDEVAAGDLEDFSLYRTLI
                     DQDPLPYSDSLHTDFLEAFGREDLREVLMRLPEIYRAPLVLRYMDGFATKEIARLMGT
                     PLGTTLARLHRGRHLFERTMWAYAEESKLLRMGEDDG"
     gene            65076..65318
                     /locus_tag="VF195_04620"
     CDS             65076..65318
                     /locus_tag="VF195_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6580135.1"
                     /translation="MGETTAAIPCSEAVRQLWDYLDHSIAPEDQEKVEHHLSFCRRCC
                     GELEFAKEIRQFLASGSSEEIPTDVKERLERFAEEL"
     gene            65322..65900
                     /locus_tag="VF195_04625"
     CDS             65322..65900
                     /locus_tag="VF195_04625"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6580136.1"
                     /translation="MTKDLMYQDEIKDAVRDAYGSITGGGGDAVAGRLYSEEELAELP
                     SGARDWALGVGNPVRYAFLQPDEVVLDIGSGGGIDTILAARRVGPRGKAIGLDVVDAM
                     IDRAGANVAEAGVEGWTEFLLGEMEQIPLPDASVDVVISNGVLNLSARKSRALAEIFR
                     VLKPGGRISIADLTVEGELPPEIANDMSAWAG"
     gene            65901..66137
                     /locus_tag="VF195_04630"
     CDS             65901..66137
                     /locus_tag="VF195_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580137.1"
                     /translation="MAGALAERVFAEKLGRAGFVDVWVGGHRPQSIDEASLYPLFTDH
                     VIELMRASLPPEQHDHVATSVIVKAHKPEAGSLG"
     gene            66350..66877
                     /locus_tag="VF195_04635"
     CDS             66350..66877
                     /locus_tag="VF195_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014151252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEX6580138.1"
                     /translation="MNIDPYVDRIHTQLDAAAEAGGEDALVLARRLAAPLDAAIRLAV
                     QEALAAAAEEITVELAPGSVEVRLRGREPQFVVTMPAPPAAADEHAGDDDLALSAIAP
                     QGGEGEDQPVARVNVRMPEHLKARVDRAADAEGLSINAWLVRAAASRLERSESGRRPE
                     RRAPSGAQRYTGWVR"
     gene            66894..67766
                     /locus_tag="VF195_04640"
     CDS             66894..67766
                     /locus_tag="VF195_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020673197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX6580139.1"
                     /translation="MPTFHTPGPIAVTLELGVGDVRIDATDRTDTTVEVRPSDPTETS
                     DVSAAEQTRVEYSNGHLLVKAPSSWRRWASRKGASIDVEIGLPVGSRLDVEAGVLTLR
                     ASGPIGETRGSLGVGDIHLDATGPIELKSGAGDISVERAAGKAVITAGSGGVRLRTVD
                     GPAVVKNGNGATWIGEVTGEARVSAANGDISIDVARAGVLAKTANGGVRLGEVSIGSA
                     VAETAFGDLDVGVRDGVAAWLDLHTRFGHVRNDLDATAAPGNDEGSVDVRASTSYGDI
                     SIHRSFAGAGRDAS"
     gene            67763..68740
                     /locus_tag="VF195_04645"
     CDS             67763..68740
                     /locus_tag="VF195_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6580140.1"
                     /translation="MTATTSAPALDVTGLRKTFGETVALDGVDFHVAAGTVFALLGPN
                     GAGKTTAVHILSTLLSADAGDVRVGGHDPARDPDAVRRVIGVTGQFSAVDGLLTAEEN
                     LQLMADLHHLAREDARRRIDELLQRLDLADEAARPVVTYSGGMRRRLDLAMTLIGSPR
                     IIFLDEPTTGLDPRSRRTVWRLVRELVDGGVTVFLTTHYLEEADRLADRVAVLNRGRI
                     IAEGTPDELKRLIPGGSIRLHFADDRQLTAAARILARATIDEDALALEVPSDASSRSL
                     RDVLDRLDRATIDVERVSMHTPDLDEVFLALTGEEPGDDGREKEGVGSR"
     gene            68737..69522
                     /locus_tag="VF195_04650"
     CDS             68737..69522
                     /locus_tag="VF195_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013415158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580141.1"
                     /translation="MNANTYVLRDSATMLRRNFRHMLRYPSVSLVVVAIPVIILLLFV
                     YVFGDTLGDGLGVASGGRGAYVNYVTPGILLMAIAGGSQSTAISVAMDMNEGIIARFR
                     TMGISRSSVLTGHVLGSTIQTMVGLVVVVGVAVLVGFRPDAGPLEWLAAVGLLGMITL
                     AITWLSLALGLFPKSVEAASNLPMPLILLPFMGSGFVPTDSMPTAIRWFAEYQPFTPF
                     IETLRGLLMGTPIGNSALFAVGWCVVLTVVGYRWAMRLYERPR"
     gene            69925..70542
                     /locus_tag="VF195_04655"
     CDS             69925..70542
                     /locus_tag="VF195_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580142.1"
                     /translation="MKLTTTTMVSVDGVMQGLGGPDEDRRGGFERGGWVTPYADDETM
                     GFLNEVYGRADAFLFGRQTYEIFASSWGTWDDPGDSPIWTALNTRPKYVASTTITEPK
                     WANTTVLSGDLVAAIRELKAKAGGELEVHGSGVLVRWLLGNDLVDEINLITVPVVVGQ
                     GTRLFPDTGPDIALELVGSQAFTPSGVTIQVYRPAGRPRYATATT"
     gene            70576..71274
                     /locus_tag="VF195_04660"
     CDS             70576..71274
                     /locus_tag="VF195_04660"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6580143.1"
                     /translation="MSDVRVIPFLVSEVRLPAHEQELLRREWWPSYAHAVVHRDGIFL
                     FDNGVGFGNTEIDATFSPRATPLDEALGQYGISLADITGAANCHLHFDHSGQNGRIPT
                     NVPIFVQRAEWDMVHEPDYTVPEWVDVPGLSYEVVSGEVEVGPGLRLIQTPGHTAGHQ
                     SLLVDAPDGTVVLAGQAVQSRAEWEGGTDPSVSGASAARHPKYADSVERLRSFDPVRV
                     HFAHDPAVWEGGER"
     gene            71271..71519
                     /locus_tag="VF195_04665"
     CDS             71271..71519
                     /locus_tag="VF195_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580144.1"
                     /translation="MSKVIVMNSVTLDGVMQGPGRPDEDTRGGFTRGGWAVAGSDELM
                     VAKMGERMGEQQAFLFGRRTYEICSPPGTPSAARSRTR"
     gene            71612..71869
                     /locus_tag="VF195_04670"
     CDS             71612..71869
                     /locus_tag="VF195_04670"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580145.1"
                     /translation="MAELKQTSSANLAVMGSGELIAALMAADLIDEYLLMIHPLVLGA
                     GRRLFGDGEHASLRLVDWTTNTKGVLIATYWPAGTGSERPG"
     gene            complement(71866..72270)
                     /locus_tag="VF195_04675"
     CDS             complement(71866..72270)
                     /locus_tag="VF195_04675"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6580146.1"
                     /translation="MTSASRPVVLHPTGQMPAALGSERRHFHDPDGRWVGWSGWIRND
                     AGDVSGWHHHAPNDTHVYVIRGSITVEFGPGGSERVVASAGDFFIVPSNTIHRELTGE
                     DSDLEAFVVRVGGEPEEVGAGGPEPAAEPQER"
     gene            72289..73032
                     /locus_tag="VF195_04680"
     CDS             72289..73032
                     /locus_tag="VF195_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580147.1"
                     /translation="MSAAVVGDEGLRRTFAWMASYVLVPGAGGDDRYWRRLVPALRER
                     GHDAIAVALPAADDSAGWSEYADAIVDAIDDRPEPVLVAQSLGGFSAPLACERKPVTQ
                     IVLLNAMIPKPGETGEAWWSNTGQREAQLEYLSTIGVTPEEASDDKVLYFHDVPPDVV
                     AEAERRGEPGQSWTPMTQRWPLASWPATPTRVLVGRDDRLFPEEFQRRVARERLGIEA
                     ETIPGGHLVALADPEGLADRLIRTAPSRP"
     gene            complement(73294..73551)
                     /locus_tag="VF195_04685"
     CDS             complement(73294..73551)
                     /locus_tag="VF195_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580148.1"
                     /translation="MVAARRPDRAVGVPRCHPVEGELRDAAIDLLGDLKGKLRSASRL
                     DRVMIGRVESVSQSPSGSSSEGTHGSPRSIFSAKTKTRLSQ"
     gene            73777..74139
                     /locus_tag="VF195_04690"
     CDS             73777..74139
                     /locus_tag="VF195_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6580149.1"
                     /translation="MTATQLELQDRTPAAVCAPIGRPKMSIHQAEATAAVFKALSDPA
                     RVRVVNLLASAEEPLCVCHLTEPLRLSQPTVSFHMKKLMDAGLLDREQRGVWAYYSLR
                     DDARRRLSVILDLEGAER"
     gene            74136..74882
                     /gene="arsM"
                     /locus_tag="VF195_04695"
     CDS             74136..74882
                     /gene="arsM"
                     /locus_tag="VF195_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007380029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX6580150.1"
                     /translation="MTELRETVRERYAAAATSVRRGMAADDVVEEACCDPADGYGLKF
                     YDITERGALPDEAVLASLGCGNPTAVADLAEGETVLDLGSGGGIDVLLSSRRVGPTGF
                     VYGLDMTDEMLDLARENARKADATNVEFLRGFIEDIPLPDASVDVIISNCVINLSIDK
                     PRVIREMFRTLRPGGRLGISDIVAANELTPEERAERGSYVGCIAGALSFDEYEAELRA
                     AGFIGISIGPTHKAADEMHSAIVRATKPAV"
     gene            75451..75807
                     /locus_tag="VF195_04700"
     CDS             75451..75807
                     /locus_tag="VF195_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6580151.1"
                     /translation="MSRVILIAWDGADWRIVDPLLEQGALPNLQALLDKGQREVLRST
                     VPTHSWSAWPSFLTGVDPDDHGVYDILETKPGTLKQYPVTLKQYPVTYRSIKARTFLD
                     DLTAAGKGQLLLDVPA"
     gene            75886..76338
                     /locus_tag="VF195_04705"
     CDS             75886..76338
                     /locus_tag="VF195_04705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580152.1"
                     /translation="MTEASEIERVYREVGDRLWWALLAYTGDREMASDAAAEAFARAL
                     ATPETIREPAAWVWRVAFRVATTLLRVPKAASDREASYEIDDRAADVILALKQLPPKQ
                     RAAFVLFYLEDRPATEIAELLEMAPATVSVHLHRARRKLRTILGDDDA"
     gene            76331..78121
                     /locus_tag="VF195_04710"
     CDS             76331..78121
                     /locus_tag="VF195_04710"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HEX6580153.1"
                     /translation="MPDPSEWISRIREVEPPELWEEAAARASSVSESDRAAVPDGHPS
                     SQPTNPRRAALVTGVAAGILALAIVVLASRTGPAEKGENRATPTPEVSSLSEAHGLLE
                     RLLGELWTAEARLAELQGSLEAAHDEVIVLQDELGPDPTDEQLREIEMLQERIQAWTR
                     SVAEDQAVVSELRDRVEEARAARARLIPPPLGGVYPDVATVTCGGDYTGGTQLSTPVV
                     RMQPDGVHFRVINGISNERVFLVIQPDGARAIPPGATLEVVIPPRPSHDVEVTCTFDY
                     PRDSWERPTHPLWIERPSTCADDDERRNAAPVDGTREVSVYFSCEENAELLGTDGQPV
                     YMFARSVPAGSSGTSDGRFESALRAYLAGPTPGEAEMGYFTPAPASLGAAIVEVSISE
                     GTATVDFDASAEGDLGNLGTSTASLTFLTELRAVAFQFPEVDSLALRVDGDCDLFWHM
                     LEMSCQTIRRADTCPTAQLAVSRLPWLPLDSGVPDPALLEDELGPVLVWFEDEDQRWA
                     GRYVALKRSSEPVVGQGLSDFPTAEVRGEIGHLVWIGDPGAGEVALVWSEGVQPCSWF
                     SLSMSSVGLTEAEAERTIRGLAAYVGERSP"
     gene            78223..79794
                     /locus_tag="VF195_04715"
     CDS             78223..79794
                     /locus_tag="VF195_04715"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6580154.1"
                     /translation="MSRVILIAWDGADWRILDPLLEQGALPNLQALLDRGQREVLRST
                     VPTHSWSAWPSFLTGVDPDDHGVYDILETKPGTHKQYPVTSHSIKARTFLDDLTAAGK
                     NQLLLDVPLTFPAPDINGNLLAGGVLPKGRTFTHPADLPDKLAEHGLEWPINGMSWTT
                     YRNRPDAYLDEAFEVTAKRIAVSEWLMDNSDWDLMASVWVSIDRTQHALSNYIAPDHP
                     DYTKNKDTRIGKKVADIFRQTDDAIGRFVSRARPDDLILFISDHGFQSCTRTLHMDHL
                     LKQFGYLEFSASQALFGPMQWGPMRKVARKVYDMLGLHGKVSLPQSVNWSKTKAFTTI
                     RSTGEGVSVNLAGREMDGTVDPGDYERVRDEVMDRLGSFVDPKTGKKPIKAIHKREEI
                     FKGEFADRAPDIMLVPAEGYSLAHARTGLEDADWVSGDHRLEGTIVAVGPNVKPFESQ
                     PALIDMAPTLVTALDAPTAVEPKGRILHEIVGSEKALTARESTVQIPGMGESAVSDNE
                     ADEMEEHLRGLGYIE"
     gene            79804..80484
                     /locus_tag="VF195_04720"
     CDS             79804..80484
                     /locus_tag="VF195_04720"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580155.1"
                     /translation="MDRPFRVGDLVEYAGPETHHTNACPGEYGRLVTPEAGGDPPPEW
                     VVDFDRVIGVYSASDLRPVELDFEVRRVDPTGVDRARIAEFLEERATARVARLGELIN
                     ATEQEALIAEKDGRIVGVLTYIVEGDACEVLTLHVAERWVGIGSALLREVERLARAAG
                     RRRVWLITTNDNLDALRFYQRRNFNLAALHVGGIVRDRKVKPEIPAIGEYGIFIHDEL
                     ELEKRLRL"
     gene            80710..81720
                     /locus_tag="VF195_04725"
     CDS             80710..81720
                     /locus_tag="VF195_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4325 domain-containing protein"
                     /protein_id="HEX6580156.1"
                     /translation="MSKRKAQAIPDLIRLLVADRESVAAGEVAKAAGVTRQAAHYHLV
                     RMVGAGELRRVGAGRGSRYFADADLDRSYALEGLEEDRVWDEVVRDVPQIQGAPANVR
                     SIMRYAFTELLNNAIDHSGGTEAWVRVWARDPLAMEILDDGVGAFRHVRDRLALPDDL
                     AALQQLSKGRETTAPDRHSGEGIFFTSKAVDRFELDANRLRWIVDNVRDDQAVGDAPA
                     HPGTRVRSEVDPATPRTLQEVFAPYVDEETLRFERTIVQLTLFETEGRFVSRGEAKRL
                     GTRLERFREAVIDFEGIDEVGLAFVDELFRVWAGQHPGTRLVPIEMSPAVERVVRRAI
                     AT"
     gene            complement(81805..82293)
                     /locus_tag="VF195_04730"
     CDS             complement(81805..82293)
                     /locus_tag="VF195_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580157.1"
                     /translation="MQTETRNGRAGVKGRIPEMTERKPVAAMPAEARSGGYLFGALAL
                     IAIWAAVALAAIYAPDMVTGTTQEHFPLALVIGSLAGLVATGSVVRGMIRGLGGMTRG
                     LTYALLMVAIWAAVALVAIFVPEMVTGTDPTRLPLAAVIAPIAAAILTRIVTDVFVIE
                     RR"
     gene            82496..83731
                     /locus_tag="VF195_04735"
     CDS             82496..83731
                     /locus_tag="VF195_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580158.1"
                     /translation="MRRPRAGSWLVIGLLAVMVLASCAEVEPPASPSPAASASVTLSP
                     SATPSPPVIPASWRRIAPLPLGGRWDEAAVWDGEEVLVFGRKQLQREPYCRYITYSYD
                     IADDAWTRLPGAPGPAGCYEGSDEVVWTGSEALLWGVTNTAYDPETNTWRKLPRPPAG
                     FGGPSMIAWTGTEMIGWGGGCCGGADATGAAYSPATNSWRRMPPSPLSARHAAGVWTG
                     TELILAGGQGPDFTSGPPTYADAAAYDPIGRTWRSIAPMPSPRYASAAWWDGTEAFFL
                     GGRRFGTTVGGWTEGLLRRGVAYDPSTDRWRRIAAMEVPRQGEVSVWTGDQLLVWGGI
                     GESGSIPPHGESYDPLTDTWTPLPRSPLRARTGAITAWTGSEMLIWGGEDARLSDWTG
                     SEPPSGAYPSDGAALTPAG"
     gene            84394..85404
                     /locus_tag="VF195_04740"
     CDS             84394..85404
                     /locus_tag="VF195_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580159.1"
                     /translation="MRDRRSTSPEEPKVQPPPARSAEEAASLVQRLDVWVAERVISRA
                     DADAIAAFESGGGGPRRGVTPLTEALAYLGAALAVAAGGTVVGGEWGDLATAGRIAVP
                     GTIWLVLFAAGSLIRRATSPPKVRLARILWFLSAASLAWTVGSVVVDGFRAERWPLWS
                     GAAAVFFAGVLYLARPSTLQQLALVGGLILLTVGLTEDSQVANGIVYWVVGSAWIFLG
                     WRRVLVQPGAASTVGSLLVLVGALLISVQHEEIGAWLSVLTAAGLIGVSVGIRHTSML
                     VLGAIGLFFSTFGTIQQYLEGTTGIAIGLLVAGVVVLALALIVSRLTSPSRWSIDPGP
                     AS"
     gene            complement(85517..86143)
                     /locus_tag="VF195_04745"
     CDS             complement(85517..86143)
                     /locus_tag="VF195_04745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580160.1"
                     /translation="MGQEERYRSATWDGAQRRVLVTLLFTDLVGSTRLAATLGDRRWS
                     LLLRAHHGRVRDLLDRWGGSEVDDAGDGFFATFDAATPAVLCATAVAVSLAELGLKVR
                     AGLHTGECIRDGGKVRGIAVHAGARVVARAAPGEVLVTRTVRDVAAGSDLLFEDRGSH
                     ELRGLPGQWQLYAALDGSGVSTENRNVHRHVSHVPRRAKRVGSLARTP"
     gene            complement(86555..87439)
                     /locus_tag="VF195_04750"
     CDS             complement(86555..87439)
                     /locus_tag="VF195_04750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6580161.1"
                     /translation="MAPSSAFDPVEFKRNIRTEWRSAAGGWRRWLDVVEGPEGGQRHS
                     AKLIELAELRAGDRVLDVGGGYGEPSLSAARVVGPDGRVVCTDISEEMLATARDRATE
                     AGIHNVEFVARDAEDLDYDDETFDAVISRATLMFLPDVPGTLRRLHGFLRPGGTLAAS
                     VWGPIPAVQFAAAFPIVSQELELPPPPPGRPGAFALSDLAKLAALVTDAGFRDVETGT
                     LPVIFETDSPEEFTDFIRDVAPQLTTMLAGHPTSVQERVWGKITDAYRPFQDASGRVR
                     TENQANLVKGGKGADGPR"
     gene            87529..87882
                     /locus_tag="VF195_04755"
     CDS             87529..87882
                     /locus_tag="VF195_04755"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6580162.1"
                     /translation="MKKYGTVDEYLADQPDEVRETLERVRRSVLAVVPKATEKIGYGM
                     PGFYVEGRPLVYYSAFKEHCSLFPASAAVIERLSDDLKGYGLAKGTIRFPIGKPPPAP
                     LIRKIVKAKLEELRG"
     gene            complement(88014..88784)
                     /locus_tag="VF195_04760"
     CDS             complement(88014..88784)
                     /locus_tag="VF195_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580163.1"
                     /translation="MSGQRSTVSGLSASVLAMLLVASCSARDPEPADPTEETSTPSLS
                     VQLLTGSDCPLFDCQGALDPGRYRAEYFDPTIEFEIRSPGWTWSYSGSFAMIADESHQ
                     DLYASDGIYFLRDPAIASQDCGDEKATESVEPGVGSSVRELVAWLEATPSLDVSEAVP
                     VTVGGLDGMLLDLRLDPGWKQTCFYSDPMPTAPLIISKADVGGYNWAMLPGMSLRWYV
                     LDSGDGVMVIDIEDGPGGASHDDLFRSGGQIVGSFRFS"
     gene            complement(88878..91691)
                     /locus_tag="VF195_04765"
     CDS             complement(88878..91691)
                     /locus_tag="VF195_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580164.1"
                     /translation="MAPEPGGLVGREPELAALSRFVRDLSEKPQDLLLEGEPGIGKTV
                     LWRAGVEAAQEAGHRVLVSRPSENETGLSYSGIGDLIGDVFDEALAEAPNLQRQALEI
                     ALLHRETGALRPDQRAISLGVLGAFRTLSASGPVVIAIDDLQWMDGSSARALSFAIRR
                     LEGHPVAVLATTRLEHGPSETLDLARELSDRGAVRTHVGGLAVDDVARILRERMSRSV
                     TPPLVALVHEAAHGNPFFSLEIVHALRDAEPAAGRPLPVPGDALDILRARIDGLSASA
                     RDVMLLMAAMARPTLATLRATSREPAMVDAGLLEAEDAGLVVAQGERLDFAHPLMRST
                     AYWSASTGRRQAIHARIAEVVLDPEERARHMALMGGGPDPERASFVQEAAGHARRRGA
                     PLAAAELWELSADLTPPDDEALLFSRKRSAALERFDAGDVQRGRTMLEDLIGAATSRE
                     QQAWTRVELAARSYNDADRVDELVRAALPDVGDHEIFLPVAHMNLAWVSLCRLEPSRA
                     VDHARAAAELAERASDPTPLRLALGALAQAEALLGADPWATIDRAVAIAGDLAPGETT
                     QPTRIRGHQMLWEGRIAEAQRAIAESDLHLMETGLELMRQDTLSVLSEVECAAGEWAD
                     AARHADEGYDIIAHAGLDEIRDQMLYARAHVAALEGRVDEARTDASTGASLAAAQGNL
                     WTEVENRSVLGFIALSVGDLDGVVRALDSADRLLVTNGIVEPGAFPFIPDLAEALISL
                     GHLDRAKQVVDRLHEHGSNLDRPLALATAARCRALIAAALGDPPGALLELERAFAEHD
                     RVAIPFEYARTLLIHGETLRRMKRKREARESLEGARSRFEALGARLWEARADQALARI
                     GGRSASPTELSETERRVADVVAQGYTNKEVAERLFMSVKTVESNLRRIYRKLGIRSRT
                     ELARQHGSSVTQRQAPSLSEHQT"
     gene            91938..92603
                     /locus_tag="VF195_04770"
     CDS             91938..92603
                     /locus_tag="VF195_04770"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX6580165.1"
                     /translation="MERLPVEVVSVLVGTTWGLAAIFWVLIRRWERTGVTEARRKELE
                     GEGPEAVSPAIRAWRWMTVTITGAIPALFAVDGFVDPVEILYAPELSFFAGPDLALQI
                     VGVVLSAAGLAILIGVGRKLAVNVYRRAIDEREMMTTGAHRYVRHPFYIHFLVLPIGL
                     CLLTLNYLALLVLAAYTTLWQPTTVVGWMRLEEDELRRRYGDEAEAYLSRTGRVFPRL
                     PRP"
     gene            complement(92668..94623)
                     /locus_tag="VF195_04775"
     CDS             complement(92668..94623)
                     /locus_tag="VF195_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580166.1"
                     /translation="MKDSTSWRANGAWQLGPAPSSRRRRRGRGTLASLLILFAFALGQ
                     ASLIAVADQPTIESDGATAEATTTSEEALPPEGVTTEEVATEEVAAAEEVSAEDPSAG
                     DEGTGTVATEEPAEATGDGTSDEAAAGSAPEAAAVDTPGPPSGDGEQPIFVKGNPDCL
                     DVMTSAQFLFERKQDPPQDATITLTFGALSGTLEVAVNEGAQTFRFTLTGDFVAAGVI
                     VKGGSNANFYDYRPDGNAADTGLHAPVNPSNGKFYDLSHISFCLMEDVPPPPTPEIDV
                     EKSCPDSVDAGAEIEYTITVENTGSEALVDVVVDDTLLGDITDEFVSALSLGLAVGAT
                     ATATVTHTPAPGVVQVTNTVTATGTGAVSDTDDSDTASCQTQVTPPGPAIQIIKGGGP
                     ALVHRGDTITYQFEVTNTGDVELFDVDLTDARCDAGTIDPGVDVDGSLAVDEVWHFSC
                     THLVTQDDPDPIPNTATVRGDTVEGGGGDEVTDQDTFQVDIIHPGITIVKTVSDATVP
                     VGTVVTYTYLVTNTGDTTLFDISVDDDVIGHIGTIASLAAGASATRTGTFSVGNSPTI
                     NIGTASGTDILGRTVTDDDDAVVTPIAGSGGDGPGCCIAGGGGGLGGTPFTGSEVGLW
                     SLLAAALTLIGGLALWATRRKLEGPTV"
     gene            94681..95319
                     /locus_tag="VF195_04780"
     CDS             94681..95319
                     /locus_tag="VF195_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019585480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEX6580167.1"
                     /translation="MQPYADGDGLAADNVLEVEGIAGYFTALGLSTAAGLNAYIPILS
                     VGLISRSTDLFDLPAPWDALGDPIVLGIVGVVGVADFIGDKVPIVDHVFHAVGLAVAP
                     IAGGMLALASASAFDLDPAVAAGMGIVAALATQAGRTAARPLSTATTGGGGNPAVSLA
                     EDGVSGVLSVTAVFWPVVTAVLAVIVLGAVFLVWRKWRQLRARFRRRDVPVT"
     gene            95359..96465
                     /locus_tag="VF195_04785"
     CDS             95359..96465
                     /locus_tag="VF195_04785"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6580168.1"
                     /translation="MNAKEIARRRIGNSRLTGDRFEAPDDVVRWHLAMQAQEYGPAKW
                     SIGQRSKGVVDADVDAAVTEGTIVRTHVLRPTWHFVGRDDIRWLLALSGPRVQQGNAG
                     RIRQLGLDARTLTRAEEVVTSALEGGNRLTRDELATEFDRRTLDRGGQRMPYLLMHCE
                     LVGLIGSGGRKGKQHTYALLDERVPAHPRPLDRDDALVELVRRYLDSHGPATVKDMSW
                     WSGFTMTDIRHAIDLLGDEVASDDVDGISFVSASSERSARAPVAGGAHLLQTYDELVV
                     GYTESRFHGEAVGDLAREAWNDRTYPTGVFLLRGRVFGHWRRTLDAKRIRVAIHTYAD
                     ATAADERALGSATRRLGRFVGLPVEVEISRFRRS"
     gene            96485..96919
                     /locus_tag="VF195_04790"
     CDS             96485..96919
                     /locus_tag="VF195_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6580169.1"
                     /translation="MRTDLTIEDLDGFLEEPVVAVLATIRQDGSVLLSPVWHEWRDEG
                     FQVWSGPNDVKTRHLRREPRASIVVAESTHPLRGVEVRGTAEILTENGFETAVRIATR
                     YLGPGKGEAYVRKVGGNDITIRLEPGELRVWDFADEQEGMGS"
     gene            complement(96916..97431)
                     /locus_tag="VF195_04795"
     CDS             complement(96916..97431)
                     /locus_tag="VF195_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580170.1"
                     /translation="MDTYMIALRILHILAGVFWVGAAFTTILFLQPTAREVGPAAGPF
                     MAHLAGKKRLVDWVLRAAGLTILAGLLMYWEVSRGLDGDWLTSAQGVSLTIGALCGIA
                     AFSLGLSVVRPTIRATIAIGRDVAASGGPPTPEQGAQLQALQQRGKAVGQVIVPLLVV
                     AVGGMAAARYL"
     gene            complement(97486..98451)
                     /locus_tag="VF195_04800"
     CDS             complement(97486..98451)
                     /locus_tag="VF195_04800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006167831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6580171.1"
                     /translation="MVRIGYTLSGEEHGPGDLLLYGRIAEESGFDFLTISDHFHPWTS
                     RQGQSPFVWSVLGGLAVKTEEIPVMTAVTCPTMRIHPAIVAQAAATTASLLPDRFTLG
                     LGTGENLNEHVTGTAWPESGERLDMLEEAIELIRRLWTGDLVTERTPHYTIDRARLYT
                     IPETSPPIAVAASAPAAARLAGRAGDALISTAPEAEIVEAFRGAGGEGRPVYGQLTVC
                     YGPDEAKAKEEALEWWPNTSVPGEVGLELAEPQHFEEVAELITTEQVAERVVCGPDAG
                     AIVEKIGMFTDAGFDHVFLHQVGPRQEEFLAFAKDELLPSIDRRG"
     gene            98564..99172
                     /locus_tag="VF195_04805"
     CDS             98564..99172
                     /locus_tag="VF195_04805"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX6580172.1"
                     /translation="MARLFSIRPTWSLKGRRFQGLRGFTGKPFHPPLTDFPIVCYTLA
                     AIFDLISFFSDEMDPKAHDFFVSATFVMVAGFIVSLGAALTGFMDWWKGIPRDRNSGP
                     IGKAKHTQVWRTINWHATVMLTVTAITLIDIILRVSDFDSGQTQGSWLVLSLIAAALV
                     TFGAAYGGTLVFEYGFNVENVEEVWKESEEDHVRLPRGGSSS"
     gene            99178..99711
                     /locus_tag="VF195_04810"
     CDS             99178..99711
                     /locus_tag="VF195_04810"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6580173.1"
                     /translation="MAADRPLGYVTQTSATSAAPPQVVYETIADLRNHLDWSGDRASS
                     ETFKLLSIEAPDGPASVGTAFSSSGAADNGTFHDRSEVTVAMAPSSFVIETDAHLDRK
                     RGKPWDAHFIHRYDVAPEGDGSRITYTETIERVNYVPYWLQPGIRSIFKVYVNRADRK
                     QLGNLARLAEERSASGR"
     gene            complement(99713..100231)
                     /locus_tag="VF195_04815"
     CDS             complement(99713..100231)
                     /locus_tag="VF195_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580174.1"
                     /translation="MPHSVGDLAEVLDNLGVFVMHPEEVLALCPSGFRRVPITQGDEF
                     YLSLQGFTANQPAYAHTHPDSEEWVVVLEGAGKALLADNPVPLEGSLVIGRATAHPHG
                     FVSGDGPMTLLSIQLPRPAEPATTWDQPGETTDPIDCHSGGTCRRCYRCGGHSHNHRA
                     KRFACENCTLEF"
     gene            100333..100752
                     /locus_tag="VF195_04820"
     CDS             100333..100752
                     /locus_tag="VF195_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580175.1"
                     /translation="MTERLIIVDHVLFVVEDFPASRRLYTAALAPLGIEELNEEPNCV
                     SYGRQDLDDFSICEGSPVTTASHVAFDAADRSQVDAFFEAALAAGATTRGEPGVWIQY
                     SDRYYGAFVNDLHGNNVEAVFHSPVPITDAPERGGAP"
     gene            100905..101246
                     /locus_tag="VF195_04825"
     CDS             100905..101246
                     /locus_tag="VF195_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX6580176.1"
                     /translation="MRTDLWNYPEGTTTPLDIEGFKVRATDGEIGKIDEATYDVDSAF
                     VVVDTGPWILGRKVLIPAGMIDRIDLDRRDVEVRLAKDQVKNAPELDEVTDYRSDTYR
                     EQVGTYYSTYS"
     gene            complement(101327..102514)
                     /gene="kbl"
                     /locus_tag="VF195_04830"
     CDS             complement(101327..102514)
                     /gene="kbl"
                     /locus_tag="VF195_04830"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010983066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX6580177.1"
                     /translation="MTTETVRPALAAELVEIRGAGLEKPERVLTTPQRARVGVRGRRG
                     EVLNLCANNYLGLADHPEVIAAAHAAMEEWGFGLASVRFICGTQELHKDLEDALSDFL
                     GMEDTILYGSCFDANGGLFEVLLGEDDAVISDELNHASIIDGVRLSKAQRFRYRNNDM
                     DDLETQLKEAAGARRRLIATDGVFSMDGYLADLPAICDLAERHDAIVMVDDSHAVGFM
                     GPGGRGTPEHHGVMDRVDILTGTLGKALGGASGGYLSGPREVVELLRQRSRPYLFSNT
                     IAPAVVGGSLKAIELLTASTELRDRLEEHTRFFRAEMEAAGLDVLPGEHPIVPVMLGD
                     AALAGRMAEAMLDRGVYVIGFSYPVVPKGKARIRAQVSAAHTREDLSFAVQAFAASKE
                     ELT"
     gene            complement(102511..>103375)
                     /gene="tdh"
                     /locus_tag="VF195_04835"
     CDS             complement(102511..>103375)
                     /gene="tdh"
                     /locus_tag="VF195_04835"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010581715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEX6580178.1"
                     /translation="PLVIGHEFVGEIVEIGSNVKDYGPGDLVSGEGHVVCGRCRNCMA
                     GRRHLCADARGIGVNRPGAFAELIALPVTNVWHHDASIDRDVAAIFDPFGNAVHSALQ
                     FPVLGEDVLITGAGPIGLMAAAVVRHAGARYVVITDVNPYRLELARKMGVTVAIDVRD
                     GSLAETQEALGMKEGFDVGLEMSGNPAALRDMVENLSHGASIAMLGLPEEPFAIDWNT
                     VIFDQLTIRGVYGREMYETWYMMTVMLQSGLDIAPVITHRFPADRFEEAFTAIRTGET
                     GKVLLHWTDGP"
BASE COUNT        15961 a        35301 c        35828 g        16285 t
ORIGIN      
        1 ggtgggctac cgcttcgctg cttgagcccg ttcctccccg ggctaccgct tcgctgcttg
       61 agcccgttcc tccccgggct accgtttcgc tgcttgagcc cgcttttcca cgaagcgctg
      121 cgcggcggcg cttcagtccc cctgcggggg tgttcttggc acgctcggcg gcgatcagtt
      181 gctcgagcca cgcgatgtcg gactccgtga tcgtgcggtt gtgctcgatc agcgcgcgcc
      241 cgtagtcgtc ggtgccgccc cggccggttc gcaccgattc ggcgatcgtg gagagtcgct
      301 ccttgagcgc ctgacgacgt cgttccagca accggatacg cgtctccggg ggcaggtagc
      361 ggaagaacgc cagccgcatc cggaatcgag cgtcttccgt ctgcgtgtcc tgcggcgact
      421 cctgcaggag ctcgaggaag atctgctccc ccttggacgt gatccggtag accttcttgc
      481 gcctggcgcc tcgcacgtcg cccgcctcgg actcgatcgc ggcgtcacgc tccagacggc
      541 gcagggtcgg gtagagggaa ccgtaggaga ccctccacag gcccccgagc gaatcgccca
      601 actctcgcga gagctggtag ccatgcatgg gccgctcctt cagcagaccc aggaccgcga
      661 gttcgagcac gtcgaggttt cccctcacgt agcgccgcga tatatcggca cggtatatcg
      721 actcgatata tagcccagga cgcgcccggc gtcaaccggg gagctcgacc cgcgcgggag
      781 tgggataccg ctctcccgcg agcgccaagg cggcgcctgc cacaccgagt gccgcgcagg
      841 cgatccagag acctcgtggc caccactcgt agatggccgt tcccccgatt ggcccgatcg
      901 tgaacgccag cgagtacgtg agccccagac tgctctgata tcgcccccgg agatgtgccg
      961 gcgcccggtc ggcgatgaac gccgcggtgc tcggagatcc gaacatctcg ccgaaggtcc
     1021 acacgaacac cgccccggcc atcccgatcc ccgtcaagag gatgccgaac gctccgaacc
     1081 cgaggcctgt gaggagcaca cccagtgcga tcatccggat ccgcgggtac cgcatcgcga
     1141 tcgcggtgac cccgagctcg aagatcgcga cgaggaagcc gttcccgccc tgcaggaagc
     1201 cgtagaccct ttcggggaag cccaggtcgc gcacccacaa cgggaacgtc gaatacccct
     1261 gcgcgtagac gagccctccc gcgaggatcg ccgcgaggac cagcaggaat cccctatcgg
     1321 cgaggatcgc agcccgtgcg ctgccgatcg ctcgatcctg cgccttgctg gtgcgcgtgc
     1381 cgtgggggag cgcgaccagc gagatcaggc cgaagaccac agaggtggcg gcgtcggcga
     1441 tgaacaactc gcggaagtgc tcttctgcca agagcccacc gatcgcgagg ccgatcgcga
     1501 atgagaggtt gaacaacagc cggttgaacg cgtaggcggc gacgcggtgc tccggaggga
     1561 tgaggtcggc gatcagggca cccgaggcgg gacggtggag ctccgcgaag aacgaataga
     1621 cgacgacgat cgtgagcacc gccggaagcg atcgtgcctc gaggaggagc aggaccagcg
     1681 cggccgaggt gatcatcgag atcgcgatcg cgttccggcg gccgatccgg tccgtcagca
     1741 agccgccgga gaactgcgcg agcatcgccc cgatcccgta gccggtgatc gccagaccgg
     1801 cctcggtcac cgagagtccg ttcttcgtga ggtagagcgc gaggaacggg aagacgaagc
     1861 tcccgagccg gagcacgaac gtgcccgcga acaggatcca cgcggtgcgc ggcatcgcgc
     1921 ggaagctgtc gcggagccgt ggacctcgct cgacgggtga gctcactgcc cgccgggtcg
     1981 gtggtacccg gagacggagg gaggaacgtc gaggtccccg ctgagctgag gatcgggttc
     2041 cggctcgccg ggtcgcgtgt ggagcgggag cacgccggcc cagatcggga gccccatatc
     2101 ctcctcgtcg tccttcggtg gaccggtgcg caccttggcc gatgcttcct cgatcggaac
     2161 cgcgaggagc agggtctgtc ggtactcggc ggcggtgggc atccgggcct catcttcgcg
     2221 accgggggcg acatgcgagg tgatcgcggc cgcggccgta tggagctcgt cctcgtcggt
     2281 tacttcccgc gccgtcccga agaccacgac ggaccggtag ttcatcgaat ggttgaaggc
     2341 gctcctcgcc atcaccaggc cgtcgacgat ggtgaccgcg atgcagacct cggcgccggc
     2401 gcggagggcc ttccacgcgc gcgcggcggt ggagccgtgc aggtagatcg tgtcgccgac
     2461 gcgggcgtgg atcgtgggca ggcagcgagg ggcgccatcg ccgtcgttga aggcgacgtg
     2521 acagatcagc ccttcgtcga ggatcgcgtc gatcgtggcg cggccgtagt caccccgctc
     2581 ggggtgacgg tggacctgtg tgcgcttggt cggtgccgac atgcgggaga gcatcccagg
     2641 catgtgtcgc tctcgtcccg tctcggcagc ggatcctgtt cggcggcgct agactccgcg
     2701 gcacataggt tcacgtgccc gagtggccgc ttcggcggcc ggacggctcg gcgggcgtat
     2761 cccgtccgag cccgaagagg gaagccggtg ggaatccggc acggtcgcgc cactgtgacc
     2821 ggggagcgaa cccgacacga accactgggt attggcccgg gaagggtcgg gggagcgagg
     2881 atccgggagt caggagacct gcccgagcgc gagaggaccg tcgccttcgc gtgaagggag
     2941 acgccatgat ccaccgtcgc atccgcggca gcgccgccgt cctgctcctc ctcctcgcct
     3001 cctgcggaga gtccacgacc cctcaggcgt cgccggagac gccctcgccg gctcctgcgt
     3061 tcccgatgac catcaccgac gacgacggcg tcgaggtgac gatcgtgaag gagcccgatc
     3121 ggatcgtcac gttcgcgccc tcgatgaccg agatcgtctt cgagctcggc ctgggcgatc
     3181 gcctcgtggg tgtgtccggc cccttcgacg actatccggc cgaagcgaag gacatcgagg
     3241 aggtcggcgg cgccggcgac ttcggcgtcg acccgacgat cgagaaggtc gtctccttgg
     3301 agcccgacct gttcctgacg atccccggcg gcgaccagtg gaagcagcgg ctgcgcgacc
     3361 tcgagatccc ggtcgtcacg ctgcacgcgg acacgttcga cgacctactg accgacatcg
     3421 ggaccatggg ggtcctcacc ggtgccgtcg acgacgccgg gaccctcgcc gagcggatgc
     3481 ggtccgaagc cgacgcgatc gggcagagcg ctcaggtggt ggggcccgtc tcgtgcttct
     3541 tcgaggtcta ctacccgccg ctcacgaccg tcgggccgga cacgttcatc tacgacctgc
     3601 tccaacgagc cggctgcgac ccggtgacag gtgaggcgac gtccgattat cccgagtggt
     3661 cggtcgacga gctcgtcgcc gacggacccc aggtctacct cgtgacgccg gagtcggcga
     3721 agagcgtggc cgccgtgtcg aagcgtccgg ggttcgccgg tatcccggcg atcgaagacg
     3781 gacgggtgga actggtggac ggcgacctgg tcacgcgggc cgggccgcgg gtgatcgagg
     3841 ggctccgcga gctcgcggag gccctgggca cggcatgacc cgtcccggca cgcgccgctt
     3901 ccgtcgcacg atggtggtgc tggccgtcgc gctcgtggtc acggtcctga tcgcggtcgc
     3961 ggtgggggcg gtgtggatct ccccggggac gacgctcagg gtcctcggct ggaagctcgg
     4021 gctgaccggt catccggacg gcgtcgatcg cgcctccgag gtgatcctgc tccagctccg
     4081 ccttccccgg gtggccctcg ccgtgctggt gggggccgcc ctcggggtct ccgggatgct
     4141 gttccagggc ctcttccgga acccgctcgc cgacccggcg gtgatcggtg tgtcgtccgg
     4201 ggccgccctc ggtgcgatcg tcgtgatcgt cctcggtgga gcatcgatcg ccggcgggtt
     4261 ggtcgtctcc ggcgcggcgt tcgccggcgc gttgctgacc acgctgctcg tctacgggat
     4321 cgcgcgcgtc gggcccaccg tgcaggtaac gacgttgctg ctcgccggca tcgcggtggc
     4381 ggccacgatc agcgcgctga tgtcgctcgt gatgtcgttc tccggcgagc agatccgaag
     4441 tatctacttc tggttgctcg gcggcctcgg ggctcgcgga tgggactcgg tgagcgtcgt
     4501 ggtgccgttc gtcgtggccg gcggtggtgc gaccctgctg ctcgcgaggg acatgaacct
     4561 cgtctcgctc ggcgaagaac gtgccgggca gctcggggtc gaggtgccga gcttcaagcg
     4621 ccgctcgatc gccgccgggg cgctgctcgc ggcggcggcc gtgtcggtcg ccggcgtgat
     4681 tggattcgtc ggcctcatga ctccccatct gctgcgtctg ctcgtggggg tggatcaccg
     4741 gcgcttgctg cccgcggtct tgctcggcgg accactgttc gtggtgctcg ccgacctcgc
     4801 cgcgcggacg gtgctcgcgc cggaggagct tccgctcggc gcggtgacgg cgctggtcgg
     4861 tgctccgttc ttcctctggc tcctccggcg cgagcgtggg agggccgtcg gtgtcgcctg
     4921 aggacgcgct cgtgctagac ggagtgaccg ccggctacgg ccccagcgac gtcctccggg
     4981 acgtttcgct cgtcgttcga tcgggggagg tcgtggggct cgtgggtccg aacggctgcg
     5041 gcaagacgac gctcgtgcgg gtcgcctgca gggcgctgaa gccggcgacc ggctcggtcc
     5101 gggtgaccgg ccgggatccg tacgagatgt cctcgaagga ggccgcgcgc ctcgtcgccg
     5161 tcgtccccca ggacgtgatc ccggcgttcc cgttcggcgt gctcgagttc gtgcttatgg
     5221 ggagggcgcc gtacctgtcg agatggacgg ggggaggacc cgccgactgg gcgaaggctc
     5281 gggaggcgat ggagaccgtg ggggtccagc acctcgccga ccggccgatg gacgagctgt
     5341 ccgggggaga gcggcgccgg gccgtgctcg cccaggctct cgcgcaggac gcacctgtgc
     5401 tcctcctgga tgagcccacc acacacctcg acatccgcca tgtcgtggag ctccaccagg
     5461 tgatccgacg actcgccgac gagcgcggtg tggcggtcct cgcggtgctc cacgacctca
     5521 cgctcgccgc cgcactctcg gacaggctcg tggcgatggc gggcggccag atcgtggccg
     5581 acggccagcc ggaggaggtc gtgacccgct cgctgctgcg cacggtctac ggggtcaacg
     5641 cggacgtgga agcgagcccg gcgacgggca ggccggcggt gatcctcggt ccccccgagc
     5701 acgcgatcgc gtcgacgggg ctccgagcgc tcgtggtcgg cggggccggc cgtggggggc
     5761 cgctgatgcg cctgctcgtg gaacgcggcg tgcacgtcgt cgcgggcgtg ctgcacggga
     5821 gtgataccga tgtcgaggtc gcggaacgcc tggacatcga gcacgtctcc gttccggcct
     5881 tctcggaggt cgacgaggag gcggcgatgg tgtggctcga ccacgcggcg atcgccgacg
     5941 tggtcgtcgt ctgcgaccct cccgtcgggc cgggcaacgt gcgcaacctc gagctggccc
     6001 tggacgccgg gatacgaggg atgcccgtcg tggtgttcga cgcgatgccg atcgccgagc
     6061 gggatttcac cggcgggcga gcgacggaac tgtggacgag gcttcgtgac ctcggcgagg
     6121 ctgtcggcag ctacccggac gcggtgaccg cgatcctgtc gcacgctcct atacgttgac
     6181 ctcaccgcca ccgcggacag gtccagaggt cggcgtcgct cgccacggct gctcgctagg
     6241 actgttcctt caggtatcgc tcgatctccg tcgcgaggtc gtcgcccgag ggcatccgct
     6301 cctcgttcgc gatcgcctcc agccgggcga cgtactcctt ggtgtcgtgg tcctccgcga
     6361 tcgccgcgtc caggcgttcc ctctgcgaga gcgcctcctc tccgagcggc cccacgggga
     6421 tcgtgacgcc gagacgccgc tcgacatgct ggaggagggc caccgtggcg ggcgcgtacg
     6481 cggctgcccc gatgtagtgg gggacctgcg cgtagtagcc gacggcgggg atccccgcgg
     6541 ccgcaaccgt catctccacc gcggacagtg ccgccgcggg cacccgcagg atgccgcccg
     6601 ggcctcgctg ctcaccgtcg cgcagcgacg cgtccgtgga ggccgtcgcc agcacgggaa
     6661 cggggcgtgt gtgcggcacg gcagccggga tcgcccccag gctgatccac tcgacgaccc
     6721 cgagccgcaa cgccagctcg agcaggtcgt cgccgagctg cttccacttg tagtcgggct
     6781 ccgggcccac gaggacgagg agatctcggc cgtcgaaggt cacgtgtcgc accatgatct
     6841 cgggccactg catcctcgcg agcacgccgt ccaccacgtc caggaccggc cgtctcgctc
     6901 gatagtcgtg cagcgcatcg acgtcgaagg aggcgaccat ccggccgccg tccgccacgt
     6961 ggttcgcaca cgcggtcgcg gctccggccg cgtcgatcca gccgtcgaag gccacgatca
     7021 ggaccggcgc cgtgaacacg gggatctgct cctcgaggcg atagaggctc accctccgag
     7081 gatagccccc ggagccggaa gagccgttcc gggcctcggt gacgtatgag tgtgacgtgg
     7141 atggggttcg agcaggacga cgaggcccct tgcggttcct tgccgtcccg gcgggagcgg
     7201 tcctgtccgt cgcgctcgcg ggatgcgccg gagacgtcta cggcgagttc ccttcgccgc
     7261 cgacgcccgc gatcacggcc acaccgagtg ccgaagccga caacggcatc ctcgccgaga
     7321 tcgccgtgga cgggagcccc tgcttcctgg cggaggccgg cgatcgggtc tgggtcaccg
     7381 ctttcgacgg gaacgagctc gccgagatcg atccggaaac gaacgaggtg gtctcgaccc
     7441 aacggatgcc gggaggaccg tgcggcatgc tcgaggtgca cggcaccctg tggatcgaga
     7501 cgccgaacgc gggggcgatc gtgcgattcg accccgtgcg tggcgaggtg atcgaccgtc
     7561 tccgggtgcc cggaggcgta ttcggcctca cgtcgacgcc atccggcatc tggggcgtcg
     7621 ccggccaggg cgaggaggtc gtccagatcg atcccgggtc gggcgagatc gtcgggcggg
     7681 tggaggtgga cggaccgctc ggaggcctcg tgttcgctcg cgaacaggtc tgggtcgccg
     7741 cggccggtgc gctcgtgcgg atcgatcccc gcacccagac cgtcgttgac gagatcgagg
     7801 tcgagtcctt cgagccggag ggactcgcgg tggacggcga cctcctctgg gtctccagct
     7861 ccttcgagca gagcgttctg cggttcgacc tccggacgat gaaggtacga gaccgcttcc
     7921 cggtcgacga tgcattgttc ggcgggatcg tcctcggtga ttcctactgg gtctcgggga
     7981 acaacaccct gtaccggctc gacgtcgaga ccggcgaggt cgaggacgag ttggagctcg
     8041 tcggattcgg gccgatcccg gcggccggag acctgtggac cgtggacttc atcgccaacg
     8101 cggtcttccg tctcgacgaa cgagcccctg aggagtagcc gatcgaccta gcccttcggc
     8161 ggatgcgtcg gcatccggtc agggatacgc tctccatcga tgagcgcgcc cgaccagaac
     8221 gtctaccgcg tccacctggc cgacccggga ggccgtatca gcgtgctcga cgtcccgacc
     8281 gagcacttcg agtcgatgga acgggggatc tcgctcggaa cgaccacgat catcccgtgg
     8341 catcgggtga tcaggtacac gcgcgaggtc gtccaggccg tcgacgacgc cttcctcacc
     8401 cgtgcggaga tccgcgcctg gctcgacgac ggctcggacg aaggcgagcg gatcgtggtg
     8461 cgggcggacc gcttcgacgc cggcacatcg acggcggact tcctcgtgga gcggaccctc
     8521 gatatcgagg gcggcgtcgt gcacctcacg aagatccacg tcccgtgggg ccgggtgctg
     8581 gaatacgaac ggctcccggt gcgggtcgag gtccccagcc gaccggactg atcttcccgg
     8641 ctcactcctc acgtgcttcc ttctcgtcgg cctcttcccc ggcctcggcg gcctccttcg
     8701 cgccgacgtc cttcaggacg ctcttcaacg tggacatcgc gttcatcgcc ggcgggaatc
     8761 ctgcgatcgt cgccgtctgc aggatgatct cgcggagctc ctcgggtgtg agtcctgcat
     8821 tgagggaggc ccggaggtgc tgggggatct gggaggagcg ccccgtcgcc acgagcgccg
     8881 cgaccgcggc gatctcgcgg tcccggagcg cgaggccccg acgcgtgtag acgtcgccat
     8941 acgcgaactc cacgatcgcc cgaccgaggt cgccgagacc ttcgaccgcg gccagggcct
     9001 tctcatcgtg cacctcgagg aatcgttccc gcccccgctc gtacctgtcg tcgctcacac
     9061 gtctcctctc ttcggcgcca gccatcccgc ccagggccga tgctcggcgc tatgcatgga
     9121 cgcggaggcc atcgagcccc cggatgatgt agttgggccg gaacgtcggc tcctccacga
     9181 gctccaggtt cgggaagcgc tccagcatcc tgccgaacga gatctgcagc tccatcctgc
     9241 ccaggggagc tccgagacag aagtggatcc ccgctccgaa cgtcagatgc gggttcggtt
     9301 tccggtccag ccgcagctca tcggcctcgt cgaaaacctc ggggtcgcga ttggcggagt
     9361 cgaacagcaa cccgagctct gcccctctcg ggatctcgac cccgtggagc tcgaacggtt
     9421 cgagcaccca gcgctcgaac atcggcaacg gcgtctggaa ccgcagcagc tcctcgatcg
     9481 ccgtcgggag caacgaagga tccgcccgga gctccttcaa ccgatcggga tgccggaaga
     9541 gggcccacca cccgaggagg gtcgcgttca ccgtcgcctc atgaccggcg ttcaacagca
     9601 ggacacacgt accgacgagc tcgtcctcgg tcagcgtgtc gccctcgtcg atcacgtgga
     9661 cgagacccga gatcaggtca tcgccgggcg cggtccggcg ttctcgcgcc agcgcccgca
     9721 ggtagccgga gaactcgagg ctcgcgcgga cggcgtcgtg ctgggccggc tcggacgggt
     9781 tcagctcgta catcttcacg atgtcggcgg accacggccg aaggaggtgg tggtctgcct
     9841 cgggcacccc gagcatctgt gcgatcacga tgaccggcag cggttccgcc aacgtgggga
     9901 gtaggtcgaa ctctcccgcg cccgccagcc ccttcagcag gtcctcgaag atcgcatgga
     9961 cgcgggggcg gagggactcg acgaaccttg gcgtgaacgc cttcgcgacg agcctgcgca
    10021 cgcgtgtgtg atcagggagt tccatgtcga ggatccccgc gttgatcagg tcccagaacg
    10081 gcgcgtgcca gtcgggcggg gccggccggc gcatctcttc gtgggtcgat gtgtgcagat
    10141 acgtgcggcc gaaccgccga tcccgaagga gggcgtcgac gtccgcgtac cgggagatca
    10201 gccagtgatc ggtcgcctcg tcgtagacga cgggcgcgga gcgacggagc tcgtcgtagt
    10261 cgcggtaggg gtccgcgatg aacttcacat cggtcggcgc gaaccgcatc gcccggacga
    10321 taccgggatg agccgaggga gagggctcgc cccggcgaag gcggcgaccg acgtggcgcg
    10381 atcgggggca ggtacccgat accggcgcat cgcgaacctc ggtggtgtcc cgatcgacgc
    10441 agcggggtat cggttcaccg aaccctctat ggaggtgagg tcatggcgag caggaagcag
    10501 gtcagcgccg cgaagcggaa catcaagagc gcgcagaagg cggcgcggaa gaagcggacg
    10561 atctcgaagc tcccgaagtc cgtgcgacag gacctggggc gccaggcagc cgccgcccgg
    10621 cggcgagggg gcgcgcccgg ccgcgggttg gaggaccgga cgcgggaaca gctgtacgag
    10681 caggcgaaga cggaagggat ctcaggcagg tcccggatgg gcaagtggga tctgatcgag
    10741 gcgctgcgcc ggtcccgcta gcccggcgca gaagcatcgc caggagcggc cgccctcaga
    10801 cgggcggtcg ttccgttccc ggccggttcg tctgaccggc ctcctcgtcg aggtcagcca
    10861 acgtctccgc gccaccctcg agcggctcca gctcgtcctc ggccttctcc tcgtcggggg
    10921 cgcccggcgg gctcggtttc cgctccgcga ggtcgtcgtc ggccatctgg gcttcttctg
    10981 gctgaccgac gtccatcggg tcgcgatcgg atcctgcggc ggtcatcgtt cgcctcctct
    11041 ggtcgatgct tctcgggtcc atcctcgcag gtcgatcctc gcaggtcgat cctcgcgggt
    11101 cgatcctcgc gggtcgatac caggcgttct ctacccttgg agccctcgct caacacccgt
    11161 gccacgagcg accgtgggcc gctcgcgttc cacaccgtgg aagagggggt cagggacgag
    11221 cgtcgtgacc cccgaggcgt tcatcgaggc gctgccggaa ccgcgccggg gccggataca
    11281 tcgcggaggc gtacgaggaa cggctttcgc aggccgacat cgggaggagc tgcatcctgt
    11341 tcgagaagct cgacgatgtg gacggctcga ggagctcatc cggaagggtt cgaggaccct
    11401 tccgtcggga gcgatcgagg gatgaagacg gtgatggatc cctcctcctg gctggagctg
    11461 cgcaggtagc cgaaggagac ggacgcggcc cgaacggcaa cgtcgaggtc ggtgctccag
    11521 agcggctcgg ccgacgccgg cgtccacgcc atgtctcgct cccacacgcc gggcccgtgg
    11581 atggtcaccg cgacgtagtc gcccggcacg acgccgtccg ggccttcttc gccgaccgtg
    11641 acgttcgacg cgttcagggt gagctccgcg aggacgtcgt gatgcaggag tcccacgacg
    11701 atcatctcgc cgagcatccg gatgaacgat tcgtcgtcga catcgacctg ccagagttcc
    11761 ggcggacgct cgacccgcat gaacgcgtgc gccgtcagga gcgcgtcgag atcgatcctc
    11821 cgcggctcca ccgtgactac ctctccgaga ggagctcgat catggtccgg gcatcgcgga
    11881 tcgcggcgcc ggtagggtcg ttgttgaagt agagatacgt ctcctccgcg ccagtcgccg
    11941 agatccgtga ggcccatcgc cgcaacttgt cacggcggta cccgggaccg tggaccgttc
    12001 cctgatggaa gcggacgtac agccaccccg cggtcatggg gagggtgtgt cgcatgcccg
    12061 gcccgtcggg ccaaaccagc gcggcacccg cgtcgtcgag caggaggaag acgtcatccg
    12121 tgtaccagga tcggtcgcgg aactcgaatg cggggcgcat ccccttcgga aggacctgca
    12181 gaagcttgcg gagccgcccg atctccacgg ggaaccgtgg tggaagctgg aagaggaagg
    12241 gtccgaggcg gtctccgagg ccggccgctc gttcccagag aagctcgacg ggctcctcag
    12301 gatccctcag ccgcttcagg tgtgtgatga atcggctggc cttcaccgtg acgacgaacc
    12361 cgggcggtgt ctggtccctc cagcgctcga acgtggcccg ctgggggagg cgatagaacg
    12421 agttgttcac ctcgaccgtc ccgaaccgtt cggcgtagta cgcgagccag tcccgctgcg
    12481 gcacatcatc tggatagaag actccccgcc agtcgtcgta ctgccatccg ctggtaccga
    12541 tgtgcggccc cacgatcacg ctccctccac cttcgccctt cgagggtgtc cggaacagac
    12601 gacggtgaaa cgcgatctgc gggaagggcg tcggacggag agcgtcggca tcggcgccta
    12661 tcatggtcgt catgcgcggc gaccgtgtcg agatcgtggt ggacatcggc aacggggtcc
    12721 agacctacga gatcgaggcc acccgggcgg ggcgccgcgt agaggtcacg acgggccggg
    12781 gcgtcgtgga ggtctctgag gtcacgcgtg gcggcaaggc cgtgaggacc ggacggttca
    12841 tgaccgcgcg cgtggtcgca ctcgtcgagc atccggcaga aggtgagccc gcggtcggcc
    12901 cgcgtcgcac ccgcagggcc gtgcgcggtc aatcgtcgct gttgtagcgc gatctagccg
    12961 ccggagacgc gctcgtcggc gttctcggcg cggatctgcg ccaggcgatc gagtgcgcgt
    13021 cccgaccatc ccgcgatctg ctgctcgccg agctcgccgg agatacggat cgcgctcttc
    13081 agatcctcct cgccttcgtc gaggcgtccg aggtcgcggt aggagatccc gcgctcggcg
    13141 agcgcgtcgg ccatctccca ccgagcgccc agctccttga agatctcgat cgcgcggtcg
    13201 aaccgaggga tggcctccgc gtggttcccg aggtcctcgt cgagccgacc gcgctggacc
    13261 atcgtgacgg cgacgctgaa gcgatcgccc atctcttcgg cgagcacacg cgcctgctcg
    13321 atcaaccggg cggcttcctc cgtctggttc ttctgcgact cgttgatcga gagcgccgtc
    13381 agagctcgga cccgggccca ggtgtcgtcg tgctcctccg ccagctcgag cgctcgatgc
    13441 cagatcgagt cggcttggtc gaagtcctct cgggtccagg ggacccaccc cgcgaacagc
    13501 agcgaccgcg acatggccca ggcgtcgtcg agctctcccg cggccgcgag cgagtctgcc
    13561 aagagacgct ccgcctggtc cagatccccg tcgacgttga tcgcgatgtc gccctgatac
    13621 cggagtgcga gggcgagcgt ccacgcgtcg tcgaacgtct cgccgagctc gaccgcgcgg
    13681 gccatcgcct cgctcgcggc cgggtactca ccgagccagt agtgggcctc ccccgtgccg
    13741 gcgaggaccc gcccctcgcg gaccccccag gcgtcctcgg gcccggccat cacgagcgcc
    13801 cgggcgtagt agtcgagggc cgaccggctc tccatgcgcc gtcgggcgcg atcgccggcg
    13861 aggaccaggg cgtcggccgc gagctccggc agccggcgat ccgcggggtc gagatcgagc
    13921 gaagccagcg ccgctcgctc cagatggtcg gccgcgtagg agcgatggcc cgactccatc
    13981 agggcctgag cgatcttctc gtgcaggttc cgccgctcgc gcttcggcag gctcccgtac
    14041 gcgacctcgc ggacggtctc gtgacggaac cgccaccgtg ttgcccggcc ggcgtcgtcc
    14101 cgcacgagga tctccgcctc ctcgagacct tggagatcct cgggctccgc gtccgacacc
    14161 gtggcgagct cctcccggtc gaaggagaag atgaagacag aggcccgccg ggccaggtcg
    14221 cggagccgat gccgcaggct gtccaaccgg gcggccacga cggcctgcac ggtgggaggg
    14281 agctcccgcc cggagctggc cgcgccgtct tgccgagccc gcagcaccat accggtcgtc
    14341 tcgacgatga agaacgggtt cccgcccgtg cggctggcga tctcctcggc ttcggcatcg
    14401 gccacgcgtc ctccagcggc ttcccggacc agctcgaccg cctcgagcgg tgggagcggg
    14461 tcgagacgca acgtgaccgc gttcacggcg ccggatcccc aggctgggcg ctcctccagg
    14521 agctccggtc tgccgagcgc gatcgtgagg acccgttttg gggcggtctt ggtggcggac
    14581 gctgcccgct cgatcagatc gagcatcgga ggacggagca ggtggacgtc ctcgaagacg
    14641 agcacgacct cgccacgggc gctcagcccc tcgaccaagg cgaggaaccc gctctggacg
    14701 tcctggacga agaccgactc ctcgcgcttg ggtccgccga gcccgatggt gagacccagc
    14761 cggccggcga cccgctccgc ctcgctgggg tcgcagcagc cgtcgacgac gtcctggagc
    14821 cggcgcatcg cctcctcggg cgccatgtcg tcttcgatgc cggcgatctc acggacgatc
    14881 gcggtcacgg gggcgaacgt cgccgtgtcg ccgtaggtct gcgcgcgccc tcggacgacc
    14941 gtgacgtccg agtcgagccc ggcgagcagc tcatcggcga gccgcgactt gcccacgccc
    15001 ggctcgccca tgatcgacac gagcagtggg ctggccgtcg ccttcaccct cgccacgctc
    15061 ccgcgcagga tgtccatctc gttcgcccgc cccacgaacg ggatcgtgcg tcgggcggat
    15121 cgcgtggtga gctggtccac ctgataggcc tgcagcgcgc cggcgaaccc cttcgcctcg
    15181 acctcctgct gcgggccgaa cgagacggcg tccttcgtga gggcgtgtgt ggtggcgccg
    15241 accagcacct ctccgggctg cgccgcagct tggaggcggg ccgccgcgtt caccacctgg
    15301 ccggtgacca gcaactgccc cgtgggcccc acgcccgtcg cggcctcgcc cgtctcgacg
    15361 cccacgcgga tctcgagcgg ctcctcgaga ccgagggact gctggaggcg atgcatcctt
    15421 ccccggatgg cgaggcccgc acgcacggcc cgcacggcgt cgtcctcctg gacgtgcggc
    15481 aggccgaaca ccgccatcac cgcgtcgccg atgaacttct ccggatggcc gcgaagagcg
    15541 cggagctcct cgctcgcggc atcgaagaac ccgccgagca cgtcgcgcgc gcgttcggga
    15601 tccagccgct gggcgaggcc tgtggaatcg accagatccg cgaacaccac cgtgaccatc
    15661 ttgcgctcct cggtgccgag cgtcgtggac acgggggtgc cgcagttcgg acagaagcgg
    15721 gctccctccg gcaggggatg accgcagaac tcacaccgca tgaggctcag catagggctg
    15781 ggcaccgacc ccgtcggagc gtcgatggga gactcgagag gcatgcacat cacaacgttc
    15841 gagcgccccg aggacttccg ccgctggctc gaacgacatc actccagcga gcgggagctg
    15901 tgggtcggct actacaagaa gtcttccggt agagcgagca tgacgtggcc ggaatcggtg
    15961 gacgaggcgc tctgcttcgg gtggatcgac ggcgtgcgca agcgtatcga cgacgagcgg
    16021 tacgcgatcc gcttcacgcc ccgccgggcc aagagcatct ggagcgcggt caacgtcggc
    16081 cgggtcgcgg tgctcgcggc ggagggcagg atgcggcccg cgggtcggaa ggcgttcgag
    16141 gcccggcgcg aggaccgatc cgggatctac tcccacgagc ggcgtgacga ggccgtgctc
    16201 tcaccgccct tcgagaggcg tttccgcgcg acgaagcgag cgtgggcgtg gttcgaggca
    16261 cagccgaagg gataccgaca gaacacgatc cggtgggtca tgagcgcgaa gcggcaggca
    16321 acgcgggagc gccgtttcgc caccctgatg gaggactccg ccgccggccg caggatcgcg
    16381 cagctcaggc ggtgagtccg gggacggccg aaggccgtcc ccggaccacc gatggttctc
    16441 tctacgtgtc ggtttccacc acggtcggtg tccggtgggg cgcgtcgttc gggaacaacg
    16501 ccaggatgga tggatcgtca tccgcccccg cggacccggt cgtgaccgtg atcgggctca
    16561 ccgagtcgca gtccccgccg tcccagaacc ccttgcacac gagcggatcg atcaccagag
    16621 cgggatcgat cgcgttcagc gtgaggttcc aggttccggt gccctcgtcg atctctccga
    16681 ccgagtcaca gatcagcccg ggaacgtcac ccgagaacgt tccggtgcac gcctcgaccc
    16741 ggtaggcgag ctcgggcgcg ttgtcgtcca acccgatcgc cgaggcatcg accaccaatc
    16801 cgacgaggtt ggagttgaag ttgctgtagt cggcgaagta cagggctgca cactcgtcga
    16861 acggcgcagg caacgagagg tcgaagacgc agacctcgcc accgggtccg ggcagcttcg
    16921 ccaccaggta gtcggcttgg aggtccgcgt ccgcgaagac gccgtcggcg cccgcgtcga
    16981 cgaacacgtc gacctcgagc gtctccgtgg tgttgtgcac gccccatgcc tgcacgccga
    17041 actccaccgg ctcggtcggc tcatccgcgt tggtcaggaa ctcctgccag gtgaacccgc
    17101 cgagcgcgtc ggtcccggtg gggacccctt cggcggttcc gtcgatcgtg ctcccggtga
    17161 aggaccgtgc gccgacggcg acgaggtcct cctcgccgcg gctgcccgcc ggatcggtcg
    17221 ctcccagcag gtagaggtcg gcgtaggaga tgcccgctgc cggcccctcc gtcaactcca
    17281 tcgtggcggg atcggtcgtg agatccagga acgtttccga gagtccgttg tccgatgtgg
    17341 cgagcggagc gacgtgccag ggcagccgca gggtatcggc gggggcgccg ctctgcgtga
    17401 cctgcacggt gccatccatg ttcgggtgga agtagtacca accgaattcc tgttccggtt
    17461 cggatacgac attcgggttc accgtcaggc ggacccatac cctctgcgac cgtccggccc
    17521 ccagcgtgaa cgatctgctc ccaccgaagg tcgagccgtc ggtcgacacc ttgagcgacg
    17581 tcatggcggg atcgaagtcc gagtagcgat ccactcccgt caccgagtag ccgtgcgacc
    17641 cgctgtcgaa gttcctgacc cggaagctcc ggatcgcgct ccacttcgtg ggtgtcggtg
    17701 cgaacccgaa cgacaggctg ccgggccatg ccaccgaacg ggcgcgagcg gactggaacg
    17761 cgtccacgcg tccggagccc atcagggtgg ccgacaccgg cgtggtcagg tcgttgttct
    17821 tcatgttgcg gttcgcctgg ttcatcatca cggccttgat ctgggccggg ttccagctcg
    17881 gatggagctg tcggagcagt gtggcgacgc ccgagacatg cggagccgcc atcgaggtgc
    17941 ccgagagctc gatcgcctcg ttcccggttc ccaccgccgc ggcggagatg ttgaacccgg
    18001 gcgcggagat gtcgggcttc aggtcgctcg tcaaccgagc ggggccctcg gacgagaagt
    18061 ccgtgaacgc gtcatcgaag cccgggatga tcgccggcac gtcgctgatc gtggcgttga
    18121 ccgccccgtc gttgtaggtg gccggcgcgt tcgggctcag ctcgaggagg atcgcctcgc
    18181 cgtctgcgcc cgagatcatg acggcaggga tcgtgatctc gtccacgtgc tcgcccggtc
    18241 cgaggccgaa cggcaggccg ccgaactgcg agaccaagat cacggccgtc gcacccgcct
    18301 cctgcgcgtt gaacaccttc gtcgtcccgt cgcagtcgcc cgcgccggtc gatcccttca
    18361 agacgagcac cgtttgaccg gtgagtgaac cgccgggcag tggatcgcag aacatcgcat
    18421 ccgccggcga ctccgccggg tcgatctccc tgccgtcgaa cagatcgtcc gtgatgccgc
    18481 cggacggaag atcggcggac cagtcctggt gcaccgtcag tccgttgtcg gggagttccg
    18541 ggatggtgcc gtccgatgtg gtgttgatca ccagctgcag cgcgacgaac tcgtcgatcg
    18601 aggcggcgac cgccaccacg cctcgggcgg aggcgggcgt gccgaggatg tacggggatc
    18661 cgccgaccgc ctggtgtcct tcgttgccgg ctgaggccac gaagacggtt ccgagatcga
    18721 cgacccgctg cgcagccttc gcctcgaggg agttcaaggt tccgtagctc acgccgccgg
    18781 agaaggagag cacgtccacg gcgtcggcga cgttgccgtc ctggttcggg tcgacggcac
    18841 gctcgtacgc agccaccagg acgtcatcgg tggagttgcc gacgtcccag accttgtagg
    18901 cgtagagctt cgccatcggt gcgacaccac ggcccacctg acccgggacg cggacgccgg
    18961 ccaccgtgct ggcggtgtgg gttccgtgcc cgtcctcatc caatggatcg aaatcgggtc
    19021 gtggggtgtc gttcgaagtc tcttcgtcga ggacgtcgta gttctcgccc acgaagtcga
    19081 aaccgccgac aaccttctcc gtggggaagg ttcccgtctc gatgaaggtc gggtcgttcg
    19141 cctcgtatgc ggcaaccgtc ccggctcccc cgaaggaggc gtgcgtgtag tcgatgcctg
    19201 tgtccacgac cgccacgcgc atcccttctc cacgggcccc atacgtgctc cacacctcgg
    19261 gtgccttgat gaacggcaca ctagtggtca ggtgcttccg cacgatcccg accggctgga
    19321 ccgatgcgac gtcggtccgc tgggcgagct gaccggcggc ctgcgccgac agcctggcgg
    19381 agaaggcgtt gatgagcacc ccgtagcgga agatgacctg tccgccgagg gatcgcgcct
    19441 ggccgatcgc gccctcctgt gagcgtagcg ccgccctggc ggcgtcctgg gtcggaacgc
    19501 tggtggacgc accggcggtt ccccgcgagc gcagtgcgac gctggcggcc gaggggacct
    19561 tcatctccac gaagtaccga ccgagctgac gagctctgac ggccgcagga aggaatccat
    19621 ccgggagtcc cgcgccctcg ctgcgaagtg tcgggcgctt gccttgcggc gccgaatggc
    19681 cgactccctg gagcgtgagt acgaggacca gtcccatcac gagcgcgagc gaacgacgaa
    19741 gccgcacgat gcccctcccc atggtcacga ttgtcacgac ttgccgattc ttgctgggac
    19801 aacggcactt tgacaaatcg cgtcgaacac gctgccatcc gcgtgccacc tgcacgcgag
    19861 gtcctacgag gggcgaggcg ttcccggcat tcggccaaac ttgaccctat aagagatgtt
    19921 atagggttgt cctcgtgcca ccccagagga cgtcggccgg tcgatccacg catcgcgtcg
    19981 cgggactccc ggcgatgcgg gcgctggcgc acccgacgcg ggtccggatg ctccacctcc
    20041 tccgcgccga ggccctgtcc gcctcggagc tcgcccggcg tctcgggatc cgggtcggtt
    20101 ccgcccagta ccacctccgg tccctggaga gagcggggat cgcgctccgc gtgggggagc
    20161 ggcgccggcg cggcggcacc gagatcctgt tcgaggtgcc ccacggcctg tgggtcgacg
    20221 tggagccgga cgctccgccg gggatgcggc aggccatgaa ccgggcctac gtcgccgagc
    20281 tgctgcggcg gatggacgcc gccgcgatcg aacgtcacga cgtcgaccgc gacgtgttgt
    20341 ccaccaggga gatcgtgctc gacgccggcg ggatcgacgc cgcgagcgag gcgctgcacg
    20401 tgttcctcca tcgcctcgac gagctcgcgc tcccggcccc gacggagggc tcgacgacgt
    20461 tcacgatcgc gaccctcttc ctccagatgc ccaaggagca gacgccgaga gacgacacat
    20521 gagcgatgac gtgaccaacc cgatccgcga cgcgccggcg ttccgccggc tgctggccgc
    20581 gagaacgatc tcgcacatcg gcgacggcgt cgccttggtc gcgctcgttc tcatggtgca
    20641 ggaggagcgg agaacgggga cggccgtcgg ggcgctgctg ctcgcgacct ccctcccgcg
    20701 tttcctcggg ccgatcgccg gcgcgatcgt cgaccgcgtc gagcagcgaa cgctgatggt
    20761 ggcctgcgac atcggccagg ccgcgacctt cggcgtgatc gcctggctcg agccgtcgtt
    20821 cccggtgctg gtcgggctcg tggccgtagc ggccgcattc gacacgctgt tctcgccggc
    20881 cggacggagc gccctgccgg cgctcgttcg gccggagcag ctgatgcgcg cgaacgcctg
    20941 gatcgggatg tccctgaaca tccgggtcgc ggtcggcccc atcctcggcg ggctgctcgt
    21001 cgagtggctg gacttccgcg gtgctctcgc ggcgaacgcc ctctcgttcg tgctctcggc
    21061 gctgttcctg ctggggctgc cgcctctccg ggcgccctcc gagggcacgg aggcccgagg
    21121 cttcctgacc gtcggcatgg acgggctccg gttcgcttgg cggaaccggg tggtgcgcac
    21181 gttgatcgtg gccctcttcg tcggggttgc cttcgcgggg ctggacgacg tcgcactcgt
    21241 gttcctcgtg cgcaagacgc tcgaaggcag tccgctcgcc tatgggctcg tgacgggggc
    21301 gtacggcgtc gggatgctcc tcggctcgct cgggctgtcg tggaagggca cggcggccgc
    21361 ggcgggcacg gtcttcctga cgggctgggt cgcgggtggg gtcggcgcgg tactcaccgg
    21421 gatcgcgcct ctgatcgccc tggtggccgt cggccaggcg atcgccggcg tcggcaacgc
    21481 ggtggaggtc gtcgcgatgg acaccctagt ccagcaggcg gtgccgcgac agatgctcgg
    21541 acgggtcttc ggcctcgtcg gcacagctgc gcccgcgggg cacacgcttg cgttcgccgg
    21601 cggcgggttc ctcgtggacc tcttcgggcc caggatcgtc ttcctgatcg ctggactcgg
    21661 cgtgctcctc gtgttgatcc cggtgatgct cgtgctccgg agggccgatg ctaccgggac
    21721 cgaaggaccg tcagcctgac cggcctcgtc ctcccgatcc gtcgcggaga acccggtaga
    21781 gggccgtcgg ctcggcgatg cccttgagct cggtctgcgc gaagggctcg aatcggaacc
    21841 gaccgcccgc gagcgcctcc acgaccgtct cgctcgtgag cacctccccc ggactcgcgg
    21901 cgtccgcgat ccgtgacgcg aggttcacgg tccgcccgaa cagatcgagg tcccgttcga
    21961 tgacggggcc cgcgtgcacg cccgcgtgcg cgcgggtcgt gccctcctcg tccatggcgg
    22021 ccacgatgcc gagcgcggcc gacaggcccc gctcggcatc gggcagtcgc agcagcgcgc
    22081 cgtcgcccag caacttcacg agtcgcccgc cgtgctccat cgcgaccgcg tgggcctgct
    22141 cctggagcgc cacgaccgag cgcaccgcga tccggtcgcc ccgtgcctcg gtcagctcgg
    22201 tgaaaccgga caggtcgacg aacgccaccg ccgggggcgc cagtggctcg ggcatcggcg
    22261 tgaggtcctt cagggccagg aaccgctcga agccttcgac gatcccctcg acgctccgac
    22321 gctcgaggta gcgggcgctc aaccacgtga acaactccgg cacgaggttg gcggccgtcg
    22381 tgtacttgcg tcgcacctcg tccgggaagc ggtcgatctc tcggctggcc agccgttcgc
    22441 gggccggctc accgaactgc tcgtcgacga gctcgaccca tccgagcgtg gcgatgcgca
    22501 ccccatcggc gagcagccgc gccgccctga tcatggtgtc gtcgccgggc gccgcgcccc
    22561 acacgtccag gaaccgccgg agcatgtcct cctcatcgac gtggatcggc gcgtccgggt
    22621 cgggcggcgg ttgccccatc gtttcgaaca ccgccggcag ccgtcccgac gccgcgcccg
    22681 agtcggcgac gaacgctccg aacgtgcgag cggatcgcgg gccgggctcg tagggcaggt
    22741 actcaccggc gaactccatg ttcagccagc cctctgccac cgaccgggag agcatcgcct
    22801 cggagaggcc agcgtcgagc agagccgagg ccatcttcac tcggaacacg tccgcccaga
    22861 cgaacgaccc cggaccctcg ggacgcagga tcccgacgtc ggacagccac tcgacccggt
    22921 gctgggacac gcccgcctcg tcggagagct cctgcgcgga gaaggttcgc ggcacgagca
    22981 cagcctagtg acgccgtcgc tgtggcatcc tgacgagccg tgagcgaggc gaccaagggt
    23041 gtggggatgg ccgcgctgac atcgttcagc caagccttcg agggagagat catcctgccg
    23101 ggcagcgccg gctacgacgc cgctcggatc gtatggaacg gcatgatcga ccgacgtccc
    23161 gccgtcgtcg cgcgcgctgc cggtgtggag gacgtccgcc gaaccgtccg tttcgcgcgt
    23221 gagcaggaac tgccgatcgc ggttcggtgc ggcggacaca gcctcgccgg gttctccacg
    23281 tgcgatgacg gcgtcgtcct cgacctctcg ctgatgcggg acgtgcacat cgatcccgcg
    23341 cgacgtaccg cgcgggtggc cggcgggacg ttgctgtcgc agctcgacgc ggccgcccag
    23401 gcccacggac tggtctgccc ggtcggggtc gtcgggcaca cgggtgtcgc cgggctgacg
    23461 ctcggcggcg ggatgggcag gctccagcgc cacttcggct tctcgatcga caaccttcgt
    23521 tccgtcgagc tcgtcacggc ggacgatcgc gtgctcaccg tgagtgagaa cgaggagtcc
    23581 gagctcttct gggggatccg tggggccggg gcgaacttcg gggtcgtcac atcgttcgag
    23641 ttcgatctcc acgagctcgc tcccgtcgtc acgcaggggt tcgtcgactt cccggccgag
    23701 cggggccacg agatcgcggc gcgcgtccgc gagtacctcg agacggcgcc cgacgaggtc
    23761 atggtgtcgt tcggggtgtc gacggggacg gaagaggatg gtgcggacct cgtcggtcga
    23821 cccctcgtct acgtcggggc cacgcattcg gggtctcccg acgatgccga ggatgacctg
    23881 cgtccgctcc gggacctcgg gccggtcgcc gatacgttcc ggctggtgcc gtacctggag
    23941 ctacagacgt ccggcgacga ggacatggcc tggggcaagc ggttctacat gaagggcgcg
    24001 ttcctggccg agctctcgga cgcgttcgtg gatgccgcgg tcgagcacgc gaccacacct
    24061 cacggcgcgg tcacactctg ggcgcagggc ggcgcgatct tgcgcgtatc cgaggacgcg
    24121 atggcgttca cgggccggga tgccgcgttc tggatcggtg tcgaagccga gtgggacgac
    24181 gccgctgagg acgagacgct gatcgactgg ggtcgcacga cgatggccgc cttcgagccg
    24241 ttcaccgcgg ccggtcacta cgtgaacgac gtgatcgaga ccggcggaga cgtcgtccgg
    24301 agtgtctacg gcgacaccaa gtacgagcga ctcgtcgggg tgaagcgagc gttcgacccc
    24361 gacaacgtgt tccggctcaa ccagaacatc gagccgtgac accgatcagg ctcacgggcc
    24421 cgcgggtcac gctccgatcc acgacgccgg aggacgccac gcccctcact ccgatccgga
    24481 acgagcccga ggttctgcga tggtggggtc atctcgagcc cggcgagatg gacgagttcg
    24541 tggcggacga gcactcgctc gtgatcgagg tcgacggctt ggtcgtgggc gccgtccagt
    24601 acggcgagga agaggacccg atgtatcgga acgcgaacat cgatgtgtac ctcacggcgt
    24661 ctcggcacgg ggagggactc gggtcggaag ccgtcctcgt gctcgccagg tacctgatcg
    24721 gggagcgcgg tcaccatcgg ctcacgatcg atccggcggc ggacaacgcc cccgcgatcc
    24781 gctcatacga gaagatcggc ttccggcccg tcggggtgat gcgctcctac gagcgagggc
    24841 cggacgggac ctggcacgac ggtctcctga tggacctgct cgccgacgag ctcccaccag
    24901 actgacggat gcttcacctc cccagggtca cccgggtgcg tcggtcggga tggctcgtgg
    24961 gatcgagagc ccagtgttcc gaggcggtcg ctcggtacag ccgatagggc gccggcgcga
    25021 gcacgtcctg ccgtgcccat gctcgaccgc cgtgcaccac agacatgcgc gagaaggtgt
    25081 cgatgccccg atcgacctcg ccctccgaca cctcctccgc gaaggcggcc atgtatacgc
    25141 actggccggt accgatgggc acctgaggat cgaagatgac gacgctcacc tcgggtcgca
    25201 gggtgaggtt gcgagagtgg gtcgcctcgg gtgaggagac ccagaagaac tccgtgtagc
    25261 cgtcggctgc gaagtacagc ggcgagaccc agggccggcc cgatccgtcg gccgtcccca
    25321 gcaccatgta catgttcgag tcgatcatga gcctggcgac ggccgccaga tccagctggt
    25381 gatccacaga tcctcccgct ccttggggct cctaggatgc ctcgttcgcg ggagggatgt
    25441 tctggttcag ccggaagaga ttggctgggt cgtagcgccg cttgatctcg cgcaggcgat
    25501 cccaagttgc gcccgggtaa gcggcgcgaa cgcgctcctc gccttcgtcc tcgaggaagt
    25561 tcacgtacgc gccagtgtcg tcctggcgga gaacgtcggc gaacgcctgc acccactcct
    25621 cgtatcgcgg acggtcctcc atctgttcga agagcgccgc aacgttcacc atgatcttgc
    25681 tcgtccggtg cgcgaacgcg gtcgcgtccg cggggacgcg cgccatctcg ccgccgagga
    25741 cgcggaactg ggccgccgcc atcatcgcgt ccgacgttcg gatgcgctcc agcatcgtcg
    25801 cggcgacgtc ctggtccaca cgatcgagga acatcgtccg gtccgacgcg atcgggtgga
    25861 agtccgattg ctcctccatg tagatcccgg ggtatggcat ctccttcagc atgtcggcga
    25921 gcggctccgc gagctcgcgg aacggcgcca ccgcacgctc gccggcctca ccgcctccga
    25981 tgtgcacgat catccccaag atcacgacct tgccgtgcgc tgcctccggg aggaacggca
    26041 tcggcggcgc cttcatcacg gtcgcgatcg tcgagagctc ctcgggcgcc gcctgcgacg
    26101 cggtggccca gcccgcgatc gtctcgggcg tggcgggcag gatcaggatc cctcccacga
    26161 tcgacggcac ctcgaacaag tggaaacgga accgggtcgc caccccgaag ttgccgccac
    26221 cgccgcggat cgcccagaac aggtcggggt gagatcgctc atcgacccga aggacctccc
    26281 cctcggcagt cacgacgtcg gctgccagga gggcgtcgat cgtgagcccg tgcttcctca
    26341 cgaggaagcc gacgccgccg cccaacgtga tcccgccgat gcccacagag ggagaatcgc
    26401 cgaacccggt cgcgagaccg tgttcggcgg ccgcctccga gtacgtgccg gccgtgaccc
    26461 ccgactcggc ccacgcggtt cggctctcgg ggtcgatctc gagcgcttgc atctccgaga
    26521 ggtcgaggac gatcccgccc tccgccacgg agtgcccaac gatgctgtgg ccaccgctct
    26581 tgacggcgag ctcgaggccc gtgctccggg cctctgcgat cacgcgagcg acgtccgcgt
    26641 cgttcgcgac ccgcacgatc gccgccggac gcctgtcgaa gccgccgtag aacacggctc
    26701 gggcctcgtc gtactcgggg tcgtcgggcg cgatcacgcg cccccggacg tccttgccga
    26761 gccggaggac gagctcgggg tcgtgcccgt tcgtggatcg catcggaacc tcctcgggtc
    26821 gtggctcttc ctgtctacaa ccggccgccg acgggtggcc actccggggt ccaccgaaga
    26881 ttttcactat cttgacaaaa agattcgtcc gtggcaacat gacacccatg ttggatctcg
    26941 aagtgataga caatcccgaa gccgccggag tgacactcga tccggtccgt tcacggctcc
    27001 tctcggagct cgccgagccg ggatccgcca cggtactcgc cggcaggctc ggactccctc
    27061 gccagaacgt gaactaccac cttcgagcgc tcgaggacca cggcttggtg gaactcgtcg
    27121 aggagcggaa gaagggcaat atgacggaac gcgtgctccg ggcgacggcg ctctcctacg
    27181 tgatctcacc cgcggcgctg aacggcgtgg agccggaccc ggcgcggttt cccgatcgtc
    27241 tgtcggctcg ctggatgatc gcgctggcct cgaagctcgt tcgcgacgtg ggggagctgg
    27301 tcgccggtgc gaacaaggct cggaagcgcc tcgcgacgtt cgccatggac ggggagatcc
    27361 gattcgcctc ggcggcggac cgtaccgcgt tcgcagagga gctagccgct tcggtcactt
    27421 cgctggtaag caagtaccac gatgagtccg cgccgcgggg tcgcgaccac cgcgtgatcg
    27481 tcgccgtcca ccccagcctg aaaccggaca ccaccaacga ggaggagatc tgagatggca
    27541 cacccgttcg agttgaccca cgagttcgag gtcgacgcct cccccgagga ggtgtgggac
    27601 gcgatcgcca cgggaccggg cgtcgacgcg tggttcatgg gcaccaacga gatcgagccc
    27661 cacgagggtg gcgcgacccg catggcgttc cgcggatcca gcgaagacgg ggtcgtgacg
    27721 acgtggcagc caccgacccg gttcgcgtac cgcacgccgg aaggtgaaga cggcgcgctc
    27781 catgcgttcg agtacctcgt tgaggggcgg gacaagggca gcacggtcgt ccgctgggtg
    27841 cacagcggct tcctcggcga caactgggag aaggagtacg agggcctgtc cgagggcgac
    27901 ccgatgtact tcgacaagct ccgcatctac ctcacgtact tcaagggcag gacggccacg
    27961 ccggtgaacg tcttcggcgg gacggtggtg cccgaccgcg acgaggcgtg gggcaagttc
    28021 cattcggccc tcggcctcga ccgggcaccc gcgctcggcg accgagtgcg cctcacgccc
    28081 gaggggctcc cgatgctcga cggcgaggtg gactggctct cccgggactt cctgggggtc
    28141 cgcaccgacg acgggatcta tcgcttcatg cacatcagcg tgttcggtgg cccgacgggg
    28201 gtcggccacc acatcttcgt cgacgggctc gaccaggcgg agacagagcg cgcgtgggag
    28261 acctggctga gcggggtgtt cgcataggcg aggggaaacg tcaaggaagc gccaaggtag
    28321 cgccaaagag cgccaaggag gtcggcatgg aaacggtgag atcgaaggac ggcacgacgc
    28381 tcgcgttcga tcggctcggt tcggggccgc cggtggtgct ggtgtgcggc ggctcggtcg
    28441 accgcatggc cgacgccgcg atcgcgcagg agctcgcgtc ggacttcacc gtcctcaact
    28501 acgaccggcg cgggcgcggg gacagcggcg acacgctccc gtacgcgatc gaacgcgaga
    28561 tcgaggacgt cgaggccgtg atcgaggcgg ccggcggatc ggcgaacctc tggggttcgt
    28621 cctcgggcgc cgcgctggcg atgatcgcga cggccagcgg cgctccggtc gagaagctgg
    28681 cgctgtggga gccgccgtac ttcctcgacc ccgatgcgag gccgccggcg gaccaggtgg
    28741 agcagtacga gacgatgatc gccgaaggcc ggcgtggtga tgcggccgcg tacttcatgg
    28801 ggaaggtcgt ggggatgccg cccgagttcg tcgccgacgc acggaaccag ccgtggtggg
    28861 cgggccagga ggcgatcgct cacacgctcg cctacgacgc gcggatcatg ggcgactaca
    28921 tgatccctac cgagacggcg gccagggtca aggttcccac cgtcgtgctc gccggcggag
    28981 cggacatgcc gttcatgcgc gagaccgcgc agacgctcgc ggatgcgctc ccgaacggag
    29041 aggtccggtt cctcgacgga caggggcaca acgtcgaccc caccgtcttg gcgtcggcac
    29101 tcaaggagtt cttcgagtga gggcgctcga gtgagggcgc tccgttgagg ttccgaacca
    29161 cgctcgagca ggcgggcaag accgcgaccg gcttcgaggt cccgtcgacc gtggtcgagg
    29221 gcctcggcgc cgggaagcgg cccgccgtgc gagtgacgat caacggattc acctaccgga
    29281 acacgatcgc ctcgatgggc gggcggttca tggtcggggt cagcgccgag aaccgcgcgg
    29341 gcgccaaggt agccggcggc gacgtgatcg acgtcgacat cgagctcgat accgcaccgc
    29401 gtgaagtcac cctgccgaag gacttcgcgg ctgctctcaa gaaggacgcg aacgcgaaga
    29461 agacgttcga cgcgttgtcg aacagcaaca aggggtggca cgtgtcctcg atcgagggcg
    29521 cgaagaccgc cgagacacgc caacgacgga tcgccaagtc gatcgccgcg ctccacgagg
    29581 gccgctctcg ctgatcccca cacccggcta ggctccctcg acatggccga cggagagacg
    29641 cccgagccgg tacccgaccg agacgaggag cgagcgagga tcgcggcgct cttggaggcg
    29701 cacgcgccgc cggactggga gcagcgcttc caacgggagc agctgctcga cgcgatcatc
    29761 gacacgacgc ccttgcagat gttcgtgatg gccgcggaca tccgcgagtc gacgctgctg
    29821 atgaaggagg cggtccagtt cgagcggttc gcgaagatca tggacaagtt cgtctccgcg
    29881 gtccgcagcg ggatcggcac accgggcggt tggttcgaca agttcaccgg cgatggcttc
    29941 ctcgcgtact ggatcgctcc ctcgacgccg aaagaggagt atcagcggcg gttcgtggag
    30001 gccgccggca acctcgcaca tacctcgcac gagctgatcg agctcttcca caggcgcgtg
    30061 ctcgaggatt tccgacggaa ctcacggaac ctctccgatg gcgtcggcct cgcgatcgga
    30121 ctcgatgccg gcccgggatt cctcgtcgag atcgcggggg agctcacgct ggtggggccg
    30181 cccgtcgtgg gcgccgtccg gatggtgaac gcggcgtcgc ggccgcagga gatcttcgcg
    30241 aacgtctacc tgggcgagca tctccacgac gagcaggacg gcatctacga ggcgctcggc
    30301 atgacggcca cgcgcgagag ccgcccgacc aaggactacc cgaagggtca ggaggtctac
    30361 gcgctgacgt tccgcgacgc cgaggggaag ccggcgggtt agcctgggcg ccgtgggcat
    30421 ggagaggcat ccgcgactgc tgccggtggc cgaggaggct cgcgtcacac cgatcgagct
    30481 gttcttcgac ctcgtgttcg tgttctcgct gacgcaggtc acggcgctga tgggcgccgc
    30541 acccacgggc cgtggagtcg cgcgcgggat gctcgtgctc tcgctgctct ggtggtgctg
    30601 ggtgggctac gcgtggctcg gcaacgtggt gcaggccgag gaagggctcg ggaaggcgac
    30661 gatgttcgcc tcgatggccg ccatgttcgt gatggccctc tcggtgccgg aggcgttcga
    30721 cgatcgggac ggcggactcc ccgggccgat ggtgatcgcg ttcgcctatc tcgcggtccg
    30781 cgtgctccac ctcgccatct tctggatggt ggccggcacg tcggaggatc gcggtctgcg
    30841 acggcagctc atccgcttca cgccctcgct ggccggtggc acggcgatcc tcctggtggc
    30901 gtcgcagctg accggaacgg cgcagaccct ggcgtgggtc gcggcgctgc tcgtcgacta
    30961 cgtcggcacg atcctgggtg gtgcgtcggg gtggcggctc cggtcggcat cacacttcgc
    31021 cgagcggcat gggctgatcg tgatcgtggc gctcggcgaa tcgatcgtcg cgatcggcgt
    31081 gggtgtgtcg gatgtcgcga tctcgtggcc gatcatcgca gcatccgtgc tgggactcgt
    31141 ggtcgcggga tgcctgtggt gggcgtactt cgacgtggtc tcgatcgtcg cggagcgcgt
    31201 gctccgccgg ctcgagggcg aggagcggac ccgcctcgcg cgcgacgcgt acagctatct
    31261 gcacctcccg atggtcgccg ggatcgcgct cgtcgcgcgc ggcatcgagc aggtcctcca
    31321 tcaggtcggc cactcgtccg acggagagct ctcccggagc ctggccatcg tgccactggc
    31381 gtcgctctac ggcggtgtcg cgctcttcct cttcgcccac gtcgcgttca agtaccggac
    31441 gtggggtcgc gtgacgctgc accgcctggg cgtggcggtg ctgatctgtg cgtcgatccc
    31501 gctcgcgacg gagctccccg cgctcgcggc gctggcggcg ctgtccgcag tcctcgtggc
    31561 gatgatcgcg acggaagcga tccgctacgc cgaggctcgg gagcaggttc gccacgagga
    31621 gggcggtccc gaggtgcatc gggcgcatcg tgaagccgac caggggtcgg cggggtgagc
    31681 tcggcgatcg tcgtcaacga cgacgcgtct gtccctcggt gcagtgatcg ttcggcggcc
    31741 gttcatcgga tcggctacca gcggtccccc tcgagcaact gcttcaggtt gtcgaggacc
    31801 atcgaccacg cctggtccga ggcatccttg gcgtcgtcgg acggcaggtt gtgctcggtg
    31861 atcgtgagct cggatccgtc gctcccttcg ttccgctccg agacttccca cgtgacctcc
    31921 tggtagtgct cggggctgtc gggcgtgccg gagaggtcgc tccagtgggt gtgcacgagc
    31981 acgtgcggcg gctcgatccg aacgatctcg cccttgtcca cgtagggctt cccctgccat
    32041 tcgcccgtgt ggacgatggg agagccttgc ttccagtcgg tctccgtgtc cacgccgaag
    32101 aaccactgct tgatcacctc cggggtcgtg agcgcgcgcc agacctcgtc caccggcgcg
    32161 tcgatcgccg cggaggtgga tgtgctgatg ccggtcttca cacccctcct ctactcagct
    32221 cggtcgcgcg gcaaacatca gctcgcgagc agctcttcga gccgatcgag gaagcccacc
    32281 tgcccgcgga ggatcttgcg ccgtgcttcc tcgagtccga accatcccgc tcggtcgacc
    32341 tcggggaact cctgggtccg gccggagcgg ggtggccact ccatctcgaa cgagttgctg
    32401 cgcacctcgc cgacttcgag gtcgccctcc atcgcccagg cgctcacgac cttgccgctc
    32461 ggctgtcgca gctcgccgag ccacacgggc tcgctcccgt caggcggggc ctgaccgatc
    32521 tcctcctcga actcgcggcg cgctacctcg agcgggtcct cgctcggttc gtactcgccc
    32581 ttcggcaccg accaggcgcc ttcgtccttc ttcgcccaga gcggaccgcc cgggtggacg
    32641 aggaggacct cgagcccggc atcgagcccg gcgtcggtcc gccggtacgc gagcagcccg
    32701 gcgctcagct tcggcaagcg cgcctcctca gctcaccgac agctcgtgga tcgcccggac
    32761 cgtctcgata gtgtcggcct cgtcggcccg cttgtcgggc cggtagccct tgacgcgggc
    32821 gaagcgcagc gccaggccgc cggggtatcg cgggctcggc tggaggccgt cgaacgcgac
    32881 ctcgacgacc atctcgggcc ggacgtgcac ggtgatgcct tcgcgaccgg tctcgatctc
    32941 ttggaaccgc tccgtctgcc acgcgagcat ctcgtcggtc atccccttga acgtcttccc
    33001 gagcatcacg aacccaccgg tctccggatc gcgcgccccg aggtggaggt tgctcagccg
    33061 tccccgtcgg cgtccgtgac cccactcggc cgcgagcacc acgaggtcga gggtatgcgc
    33121 acgtttgacc ttgatccagc cggttcctcg gcgacccgcc tcgtagggag cgtcgagcgc
    33181 cttcaccatc accccttcgt gcccgcgtgc gatcgcgtct tccaggaaac cgtcggcctc
    33241 ctccacgtcc gccgtgacga tccgggggac gcggagctct tcagggagcc gttcgtccag
    33301 ggcggcgaac cgctcgacgg cggggcggtc gatcaggtca tcgccgtcga ggtgcagcac
    33361 gtcgaagacg aacgcggaga gcgggtagtc cgtctggaac tcggtgaggt ccttccggct
    33421 ccggctcccg aaccggctca tcgtcaggcc gaagggatgc ggccgccggt cgggtcgcag
    33481 cgcgatcgcc tcggcgtcga acaccgcggc ctcgatgggc aggctccgga tcacctcgac
    33541 gatctcgggc acgcgatcgg tcacgtcggc gaggttccgc gtgaacgcgc ggacctcgtc
    33601 ccccagccgg tgcacctgga tccgggcgcc gtcgagcttc cactcgaccg cggcaggcga
    33661 gatccgtgcg agggccgcgg ggatgtcctc ggcggactgc gcgagcatcg gttgcagcgg
    33721 ggtgaggagc gagagcccga agtgggcgag ggcggcgctc cctccgtcga tggccgccgt
    33781 cgccgtcgcc cccaggtctc cggtcagcat cgcggcgcgc cggacctccg ttcgcgggac
    33841 gttcgcggct cgcgcgatgg cctcgaccat caccccttcg agggccccct ggcggatctc
    33901 gcccatcagg agaccgcgga ggaagtcctg ctcgtcgtcc gtggcccgtg cgaacagatc
    33961 gtggagcgcg gtgcggcggg cgccctggga gccggcgccg gccagagcac cgatccgggc
    34021 gagcgcggaa tcgacgtcga gcagctccaa cgtggcaggc gacggcgacg gcgacggtag
    34081 gtccttcagg gacgcccatc ccacgccgat cgacccctgc gggagcacgc cggagaggta
    34141 cgcaaccgcg atcgggacct cgccgggctc gagggcttcg aggaccgacg agagccgggc
    34201 gaccttctcg aggcgggacg aggtcgcggc cacgtctgcc gacgccgccg cgacctcacc
    34261 caggagcatg ccctttcctg cgaccctctg gcccttcgag ccggtggccg ctacgagccc
    34321 tccgggccca tcgggctcat gagctggaag tagttcccgt cgggatccgc gaacgtggcg
    34381 atcagcccgt ccatctcctc gccggggctg tactcctgga ccacggtggc tccggcggcc
    34441 acgaacctgt cgaactcgga cttcacttcg gcggtctcga cgttccagat caccctgccc
    34501 ggctcggcgt tcttcccctt cacctgatcg tgagggccga tcgtgactgc acccgacccg
    34561 atcatccagc cgatgtaccc gccctcgttc caggtcggct cgccgaaaag cttcgtgtag
    34621 tactcggcca actgctgcgg gttctcggac ccgatcagga cgctgttgaa gttcatgaca
    34681 catccccttt cctcgcccct tgactaccag acggacggcg cctcggaaac gcatcgaggg
    34741 ggttcgccta ttcgacgcct cacgaccgtt atccctctcc ggcgtcgttc catcgcccgt
    34801 tccgcttcgc ctcctcgatc gccgcgaggc cagcgtgggt catccgtccc tcctcgatga
    34861 gtcgctgcgc tcgtgctcgg ttcacggcgg accagttgct tcccttccgg cgcggcgtgt
    34921 agcggagggc gtatcgctcg tcgtcgatcg ggtgcatcgc gctgtcgatc catccgaagc
    34981 acagcgcctc ctcggtggcc ggcaccagcg ggaccgaggg cgtgctcgcg cccttcttgg
    35041 agatcgtcag ccacactccg tcctcgccgg cgtggtgctc ctcgagccac gcgcgccact
    35101 cctccacgga tccgagctgg agcagttccc ccgtgatctc catgtcagtc atcctaggca
    35161 ggcgcaccca cgacgaccct cgacgagcgc tctgagccgg ccgctcttcg gccacgaccg
    35221 ataccgggaa tcccctctgc tacgttgagc gcatgagcag ctcgttgctg gaagacgcgt
    35281 tcgggcacca cgtgtgggcg acgctgctcg tcctcgatgc ctgcctcgcg cttccgcccg
    35341 agcagctgga gacctcggtc ccgggcacgt acggctcgat cctcgatacg gcgcgtcaca
    35401 ccgtcggtgc ggatgcgtcg tacctgttcg ttctgagcgg tgggcgcgtc acgccgatcg
    35461 atgaggacgc gatggacctg gcggagctcc gctccacgat ggaagggcac ggagccgcct
    35521 ggtcggagac cctggcgcag gacctcgaca cggacgtgat gctcgtgcgc catcgcgacg
    35581 atggatccga gacccacgcg cccgcgagca tccggctcgc gcaggcgctg caccacggca
    35641 ccgaccatcg cagccagatc gcgaccgccc tgacgacgct cggggtcgag ccgccgccga
    35701 tggatgtctg ggactacggg aacgagttgg gtcgcgtcac cgagttcccg cccacctcgt
    35761 gacaacggat cgacaggcgt cgaaggcggt tgccttccgc gcgttgcacg acggcgagcc
    35821 cttcgtgatc ccgaacccct gggacgccgg atcggctcgc gtactcgagg cgctggggtt
    35881 ccgagcgctc gcgacgacga gctccggact cgccttcacg ctgggtcgcc tcgatgggag
    35941 cgtgacgctc gaggagatgg cggcgcacgc gggtgcgctc gatcgtgcga cggagctccc
    36001 tgtctcggcg gacctcgaga acggctacgg cgccgatccg gagcaggccg cgacggcgat
    36061 ccgtcgtgcc gcggacgcgg gcgccgtcgg cgggtcgatc gaggacttcg accgcgacga
    36121 acaccgcctg tatcccccgg accacgcgac cgcgcggatc gtcgcggcgg tggaagcggc
    36181 tcgcgaactc ccgttcccct tcacgctcac cgcgcgggcc gagaaccaca tccgggggaa
    36241 ccccgatgcg cgggacacga tccgccggct gcaggcgttc gaggaagccg gcgccgacgt
    36301 cctctacgcc cccggcctcg agaccatcga cgacatccgg acgatctgcg gatcggtggg
    36361 gaagccggtc aacgtgctgg cccggccggc gatgcgcctc gccgagctcg tcgacgctgg
    36421 tgcgcgccgc gtgagcgtgg gtggtgcgct cacgtggacg gccgtgaagg cgttcgccgc
    36481 cgcggccgcc gcgatccgtg acgtcggaga cctctcggcc ttggacgccg acgtgccgct
    36541 ggccgactgg ttcgggggat gagcctcgat cctggtcagc ttccgctgcg gtggcggagg
    36601 tgcaccgtgc cgttgtcgaa gcgccgctcc accgctcgag gtcgaggcgc catcgtcggg
    36661 gtcgaagctc cgttcgagcc gcgtctccgc actggtcagg tttgcgttct cttgccgcgc
    36721 cgagccgtct gctagcttcg cccggtccgt cggatggggg gttcatgcag cgcatacgta
    36781 ccgccgcggt cgtgctcgct gcggggctcg tcgtgaccgc gtgtcagagc gacacgacgg
    36841 gcgagctggt cgacgagccg cgggcagggt tgggcgggat ggggatcgtc ccctccggac
    36901 ctcccaccga gttgctgggt cccgagttct tcccctgcca tcctggacgc ggcgacgcga
    36961 gctacacgaa gtacggcgtc ggccggatgc gccgcggcgg tctcgagcgt gaggaattgg
    37021 agccggacca catcgtgatc gacggccacg gggtcaactt ccacggcagg gatcgggtcc
    37081 tcgacggcgg aacgatcgag atggagatgg acgaggacta cttcggaccc accgtgctga
    37141 ggggccaggc cggagcaacc gtcacgatcg agctcgagaa cgaaggggtt cgccagcaca
    37201 acttcagcgt gcggggtcag gggatcgacc tgacgtgcgg cgtgcgggcg cgagatgagg
    37261 tcgaggtcgt cttcccccgc tcgggcctgc tcgcgttcac gtgcaagttc ggggaccgat
    37321 cgggcatgcg aggggcgttc gtcgtcgagg ggtgagcgtc gcccttcgct acgctgaggc
    37381 tcgatggagg cgatcgtcca ggaccgctac ggctcgaccg acgtcctcga gctccgagag
    37441 atcgaacggc cgagcgcagg cgatgacgag gtgctcgtgc gggtccatgc ggcctcggtc
    37501 cacgcggacg tgtggcacgc gatgaagggc gtgccctacg tcctccgggc gatgggctcc
    37561 ggactgcacg caccgaagca ccacgtcccg ggatcggatc tggccggcgt cgtcgagtcg
    37621 gtcggctcga acgtcacgag gttccagccg ggggacgagg tcttcggcct gagcatcagc
    37681 gccaacctgt ggcgcaacgg tggcgcgttc gcggagtacg cagcggtccc ggtaaccacg
    37741 ctcgagccga agccgacgag gctcacgttc gaacaggccg ccgccgtccc cacgtccgga
    37801 tcgctcgcgg tgcagcacgt gcgcgacgag gcccggatcc gatcgggaca gacgatcctg
    37861 atcaacggcg cgggcggcgc cgtggggagc ttcgcggtcc agctcgccaa agcgtgggga
    37921 gcgacggtga ccgccgtgga tgccaccgag aagctcgaga tgcttcgttc gatcggcgcc
    37981 gaccaggtcc tcgactacac gaaggaggac ttcacccggc gcgatgagcg atacgacgtc
    38041 gtcttggaca tcgccgggaa ccacccgtgg cgggaatgcc ggcgcgcgct cgctcccgac
    38101 gggacctacg tgctgagcgg tttcgaccag tacgggcggt cgggccatcg ctggttcggc
    38161 agcctggggc ggttcgccag gctgatcgtg atcgctccgt tcgtgagcgc gttgcatccg
    38221 ttccgtggcg ccaaggatcc gggtgatcga ctggtcgtcc tgaaggagct catcgaggcc
    38281 gggaaggtca cgcccttcat cgatcggacg ttcccgctga gcgaagtccc cgaggccatc
    38341 cgctacctgg agacaggaag ggctcgaggg aagatcgtcg tcacggtgtc tcccggttga
    38401 caggctaacc ctgttcgcct aagctaacgg cgttagccaa cttgaggagg ccggcgatga
    38461 gggagctcag cacgcagatc gagttcgacg ggacgcccga tgaggtctgg gaggtcctcc
    38521 aagacctcgc cgcgcacgcc gaatggaacc cattcatcac gaagatcgac ggcgagctgc
    38581 gaccgggcgc gaagctcgac gtcaggctcg agcccgaggg cgagcgcggg atcacgttgc
    38641 acccgacggt cctcgcggtc gagcccgggc gcgagctccg ctggctcgga cacctcctcg
    38701 tgccgagggt gttcgacgga gagcaccggt tcctgatcga ggagatgggt cttggccgcg
    38761 tgcgattcac ccagagcgag cgcttcggtg ggatcttgct gccgctgttc tggaagaagc
    38821 tccgggacgg aggaaccgcg aagggattcc gcgcgatgaa cgaggcgctc gcacggcgcg
    38881 tcgccgggct ccacccgcag gcggtctgag tgatcgtcgc tcccgaacgc agcgcgaggg
    38941 tggcagagat cgtcgccgag gctcgcgagc ttctcgagcg cgagggaccc gagggcctga
    39001 cgatgcgacg tctggccgag gcgatgaaga tccgggcgcc ttcgctctac aagcacgtca
    39061 gctcgaagga ggacctcgag gcactgctga tggccgaggc gttccgcggg atgggcgcgg
    39121 agctgcacgg cgcgatcacg ggcgcccgga aaggcgggtc gaagacgacg gcgctcgccg
    39181 agctcggtcg ggcataccgc cggtgggcgc tcgcccaccc gcacctgtac cggctcgtga
    39241 ccaacggacc gctgccgagg gagctgctcc ccgagggcct cgaggcgtgg acggccgagc
    39301 cggtcgtcgt cgccgtcagt ggcgatcccg atcgggcccg cgtggcgtgg gcgttcgcgc
    39361 acggcatgac gatcctcgag ctcgacggcc gattcccgcc cggcgccgac ctcgatgccg
    39421 catgggctgc agggatcgat gccctgaccg aaccggaacg gaagagctag ccgaagcgcc
    39481 gcaggaaggt gagcgcgagc gctacttctt gccgaggagg gtgccgaaca ccgtgcgaag
    39541 ggtccggcgc ccgtcggccg tcgtgaacag gtccttcgcg aggccgatcc cgatgtcctt
    39601 cgcctggtcg cccatcgact tctgggacgc cttcccttcc ggacgaggct tcggtcgacc
    39661 cttcgatttc gatcttgaac cggctcgacc ttccggtcgc gtcgtccctt ctcccgccgg
    39721 cggaggtgga gggagcggcg gcgcgtccgt cgtctccgcg gcgcccgcga gcttgcccat
    39781 gaggatctcg tgtgcgctct cgcggtcgac ggacgtgccg tacttctcgc ggagcgcccc
    39841 ggcggcgatc agctcggagc gggtcgtcgc atccaccgca tccatcatgg agtccggtgg
    39901 cacgaggcgc gtcgccgcga gtggtgtcgg cacgcctttc ggggtgagca cggtgacaag
    39961 ggcctcgccg atccccaggg aggtgagcgt ctccccgacg tcgtagtggg cggtgatcgg
    40021 gaaggtcttc acggtcttcc gcaggttgtc ggcatcatcg ggcgtgaacg cgcggagcgc
    40081 atgctgcacc cggttgccga gctgcgagag cacggacgag ggaacgtcgg tcggagattg
    40141 cgtcacgaag tacacgccca cgcctttcga gcggatcagc cgcgccgtga gctcgatctg
    40201 gtccatcaac gcatcgctgg cgtcgtcgaa caggaggtgc gcctcgtcga agaagaacgc
    40261 gagcttcggc ctggggaggt cgccgacctc gggcagcgtg gcgtagagct gcgctagcat
    40321 ccagagcatg aacgtcgaga acaatcgggg acgatccatc acgtccgcga tctccagcag
    40381 agagatcacc ccccgcccgt cctcggtcgt ccgcagcaga tcgtccacga cgaactccgg
    40441 ctcaccgaag aacgcgtcgg ccccggcggc ctcgagctcc acgatcgagc ggaggagcac
    40501 gccgatcgtc gccttcgaga agcccccgta ctcctcgagg accggcgcac cctcgtccga
    40561 cgagaggaac ttcagggtgg tgcgcaggtc ggtcaggtcg aggagcggca gccgctggtc
    40621 gtcgcagtac gtgaacacga gcgtgaggac cgacacctgc gtcttgttga ggtcgagcac
    40681 cttgccgagg agcaacgggc cgaacgacga aaccgtggca cgcaccggcg cgccgagcgc
    40741 gccggagagc gacagcagct cgaccggatg cgcctgcggc tcgaacggga tgccgagctt
    40801 cgccgatcga tcgcggatcg cctcggccgt ggcgtcaccc gggcgcgaga gcccggtcac
    40861 gtcgcccttg atgtcggaga tgaagacggg cacgccggcg gcagagagct ggccggccat
    40921 gatctgcagc gtcttcgtct tgcccgtgcc ggtcgcgccg gcgacgagcc cgtgccgatt
    40981 catcatcgag agcgggagct gaaccagggc gccggtctcg acctctccgt ccttcatcgg
    41041 ccgcccgagg atcacgcagg gctcgtcgaa cgcgtagccg gatgccatct cggccgtgaa
    41101 cgcctcgtcc atggagtcgg gagcctagac gacgcgcagc tcagtcgagt cgctcgagga
    41161 cgaccacggg gatctcgcgc gccgttctgg tctgataccg ctcgtactcg ggccagatcg
    41221 agaccatcaa gcgccagagc cgaggcttcg agcgagccgt cgctgttcgc gctcgcgccc
    41281 cgaatcggtc cccggccacc tgcacgaaca cgtgaggatc ggccgccagg ttctggtacc
    41341 agtgcggatg tgccttcgcg ccgccgttcg aggccaccac gatgtaccgg ccgcgatcct
    41401 cgccgtagat caacgccgtt cgccggagct tcccggtgct tcgacctcgc gtggtgagca
    41461 gaagggtgtc gacgccgctc caccggtgcc cctccttccc atccgactcc acgtacctgc
    41521 ggacgtgctt ggcgacccaa ccggtcgggc tatcggtgac gaggtcgtcg cgagcatcag
    41581 gcacaccgct ggcgtatcac ctcgacggtg gcaccgtcac gtcggatgcg gatgttcccg
    41641 tggagtcggt cgtcgcctac tcgacgggat cccgccggcc gtacgaggag atcgggccga
    41701 ggtggccgag cttgtccggg ttggcgacgc tccggacggc ccggatgcga ccgcccacga
    41761 cttcgagcga cagcacggtg aggaggaggc cttcgctgtc gtaggcgagt gctcccggct
    41821 ggccgttcac gcgggtgagc tcgagtcgag caccgatcct cctgccctgg tcgaagagac
    41881 gcaccaggaa cgtcgcgacg cgttgggcgc cgtggatcgg ctcccgtgcc gcgtacacct
    41941 tccccccgcc gtccccaacg gcgaccgagt cggccgccag gagctcgagc agcccgtcca
    42001 tgtcgccgtc ctggcacgcc gcgaagaacc gctgcgcgag ctcgtcgcgc tcctcgccgg
    42061 acggcacgaa ccgaggtgtc cccgcctcga tccgccgcct cgcgcgagtg aagagctgcc
    42121 gggtgttcgg ctcgctcttc ccgacgatct ccgcgatctc cgcgtagtcg tactcgaaga
    42181 cctcgcggag caggaagacc gctcgttcca ccggtttcaa gctctcgagg agcaccagga
    42241 acgccatcga cagcgagtcg gccatctccg cctgctccgc cacatcgtcc tccgttccgg
    42301 ccacgagcgg ctcggggagc cacgggccca cgtaggtttc ccgccgggtc ttcgcgcggc
    42361 ggaggtggtc gatcgcgagt cgcgtcaccg ttgccgccag gtaacttttc gccgagtcga
    42421 cctcggcccc ctcctcggcg gcgcggcgcc atcggaggaa cgcatcctgg acgacgtcct
    42481 cggcctccgc gacgctcccg agcatccgat aggcgatcga gaacagcagc ggccgcagct
    42541 cgacgaattc ctcatcagcc cggatcacgc cggctccgtt ccccgtcatg tcgctcccga
    42601 gacgcgcgaa gggcccatcc cgtgacaagt ctcccggact gtcacagggc cgccacctcg
    42661 cgcgtctcgg ggatcgaagg ctcgcaaggg gatcgaaggc ccgaagaggg ctcgagaagg
    42721 agacgaagga ggacgtcatg aaggtattcg tcgtaggtgc gaccggcgcg atcgggcggc
    42781 agctcgtccc gctgctggtc gcgcgcgatc acgaggtcgt cgcgacgacg cgctcgccgg
    42841 agaagacgaa cgccgtgcgc gcgatgggcg ccgaacccgt ggtggtggat gccctggatc
    42901 gggaggcggt catgagcgcg gtgacccgcg cggggccgga ggcggtcgtc caccaggcga
    42961 cggcgcttac cggggtgttc aacctgaagc acttctcgcg cacgttcgag cagacgaacc
    43021 ggctccggac gctcggaacg gacaacctcg tggcggctgc gcgaacggcc ggcgcccggc
    43081 ggttcgtcgc gcagggcttc gctggctggc cgtacgcgag cgacggtcgg gccttgagga
    43141 ccgaggagga tccgttcgag ccgtccccgc cggaggagat gcgctcgacc gtgcacgcgc
    43201 tccggcacct ggagaccgcg gtgttgggta cggaggatct cgagggcatc gtgctgcgct
    43261 acggcggctt ctacgggccg gcaacgtcgc tcggggacgg cggcgcgatg gtcgagggga
    43321 tccggaagcg gcagctcccg atcgtgggcg acggggctgg cgtgacgtcg ttcctccacg
    43381 tcgaggacgc ggcggcagcc accgtcgcag ccctcgagcg gggggcgccc ggcgtctaca
    43441 acatcgtgga cgacgagcca gcatcatctt cgatgtggat cccggagctg gccaaggcgg
    43501 tcggcgcgaa gccgccacgt catgtccccg tctggttggc gaagccgctg atcggcgatg
    43561 ccggcgtgtg gttgatgacg aaggcgccgg gcgcctcgaa cgcgaaggtg aaggcggacc
    43621 tggagtggac accccgctac gcctcctggc gcatagggtt ccgcgaaggt cttcgggcgg
    43681 aaagagctct aggtgcctga tatcggcggg gcgtccctga gcttctcgag tccctggagc
    43741 atcaggtcga gcgcgaactc gaactcgaac tggtcgtcgc agccggcgcc gacgacggac
    43801 cggtcgtcgt gcgagatcgt cgtgtagatc tcgaagatgt gagggtgggt gtcggccatc
    43861 gcgccgaaca aggcctcgtc gtccgggtcg gcctcggagg agtcgttgaa gagctcctgc
    43921 gtgaacccga acatcctgct gcccatcgca tgaagggcat ggtgagtgag atcgagcgag
    43981 aagcctccgg tgcggaacat cccgatcatc gagtccaggt acccgaggac gaccggagtg
    44041 ggggtggtgc gcgactccat caggcgagac gcccacggat ggcggaggag cgcacggcgg
    44101 gcagaaagga tcctcgcacg gagcgcggtc ttccagtcgg ttccctcgag cggcgggtcg
    44161 atctcaccca ccacgacgtc gaccagagcg ttcaggagct cgtccttgtt ggccacgtgg
    44221 cggtagagcg ccatcgggac gacgtcgagg gcctgagcca gcttcctcat gctgagggac
    44281 tcgatgccat cctgatcggc cagggccacg gcggtgctca gcaccctgtc tctggtgagc
    44341 ggtgctcggg tctccgctcc accctcgatc ttcgtgctca acaactcacc tccggcggtc
    44401 tgcctgttga caagtgtacg ccgtacacgt actctccacc catcgaagtg tacggcgtac
    44461 acactagaga cgccagcatg gacagcaggg gaaaggatct ggatgaaagc gatcgtgcag
    44521 gatcgatacg gctcagcgga cgtgctggag tttcgggaca tcgaggagcc ggtggtcggc
    44581 gagaacgacg tgctggtccg cgtgcaggct gcgggctgcg gcccggacgt ctggcacctg
    44641 atgaccggga tgccgtacat ggcccgcgtc gcgatcgggc tccgcgagcc gaagctgaag
    44701 gtcctcggct gggacgtcgc ggggacggtc gaagccgtcg gcgcgaacgt gaagacgttc
    44761 cggccggggg acgaggtgat gggacaagcg aagggctcat tcgcggagct cgtgaccgcc
    44821 aaagccgacg aactggtccc gaagccgtcc cggctcagct tcgagcaggc tgccgccctc
    44881 cccgtctccg gcgtgaccgc cctccgggcg atccgagacg aggggaacgt gcagccgggg
    44941 cagtccgtcc tggtgatcgg tgcggccggc ggcgtggggt cgctcgccgt gcagatcgcc
    45001 aaggcgcagg gcgcgacggt caccgccgtg tccagcggct cgaaggctga gctggtccgc
    45061 tcgatcgggg cggacgacgt gatcgactac acgcgcgaag acttcacgga cggctcccgg
    45121 cactgggacg tgatcgtgga tactgcgggc cggcgccccc tgtcacagct ccgccgcgcc
    45181 ctcaccccga aggggaccct cgtgatcgtg gggggcgacg gcggcggcaa gtggaccggt
    45241 ggcttcttcc gtgggatgct ccgcgcacgc gtcgtgtcgc tcttcgtggg acagcggctg
    45301 cgcgggttgg caacgaagat cgcgcaggaa gatctcgtcg cgctcgcgga gctcgtcgag
    45361 gccggcacgg tgacgccggt gatcgatcgg acgtatccgt tgatcgaagc ctctgacgcc
    45421 atccggcacc tcgaggaggg tcacgccacc ggcaagatcg tcatcaccgt ctgacggatg
    45481 cgttcaccac gctgagggtt gtcggccctg tccgtcgaga aggaggaagc gttcgccctc
    45541 gaggagctcg gtgctcccgg ttccgtcgtc ggggtcccgg ttccagaacc cgctgaaaaa
    45601 caggttcgta tccgcgtcgc cgcggccggc ctgaacccgt tcgatgatgt ggggatggtg
    45661 tcgacggcat cgcgggaaac acccgccggc catgctcgaa cgaggggagt tcgtgagccc
    45721 cgagatcagg accgttcccc gtagcggaag ccgcggaggc gttcgagcgt ggcgacgggc
    45781 cacacccatg gcaagatcgt cgtcatcccc tgacagccag gcaacttccc ggaccctcct
    45841 cgatcgggca ggagctcagg tcgagcgact cgagcgtgag gccgagcggc tcggatggcg
    45901 ccgaacgcga acgggcggta cggtcggagc gtcggaggag cgcgtgaagt ccgctccggg
    45961 cgacccgcga cctcgagaag ctggtgggcg tgatgctgtt cgtgaggctc ggcgcgatcc
    46021 gcgtgctctc gagcgtccga tcgtgacggc ggagctccag gcgcgcgtcg aagcggcgct
    46081 ccggcgggcc ggtcgccgac ccggcctcct cgtcttgagc gcgaccgatg gcggcgacac
    46141 ggcgttcgct tcgcggggac cgctcccgga tccgccgtgc gcaccggaac gagcggtctt
    46201 cgagatcggc tcgatcacga aggtgttcac ctcgctcctg ctggcgatcg cggcggagcg
    46261 aggtgagctc ggcgtggacg accctctcgt cgagcacctc ccgcgcggga cgcgcgtgcc
    46321 gatgcgcggt ggtcggccga tcacgctcgt cgatctggcc acccacaggt cggggcttcc
    46381 tcgactgccg cccgggttcc tcctgcacgc gctccgtcac cgcgacgatc cgtacgctcg
    46441 gctctcgacg gagggcgtcc tcgcagcgct ggaggagacc cgtccgcgag tccccgcagg
    46501 cgagcggttc cgatactcga actacggcgc gggcgtgttg ggcatcgcgc tcgcccacgc
    46561 ggccgcgacc gactacgaag ccctcgtgcg ggagcggatc accgcgcccc tcggcctgcg
    46621 ggacaccgtg atcaccctga gcgaggatca tcggagccgt ctggccgcag ggacgaaggg
    46681 gtggggcggt ccggccggcc tctgggcgat ccccggcctg acgggagccg gcgcccttcg
    46741 atccacggcc gccgatctgc tcaccttcat ccgcgcccag atggggacgc tcccggacgt
    46801 gcccgaggag ctcgcggggg cgatccgctc gagccacctg gctcgggctg caggcggtcg
    46861 cctaccccgg ggatgcgcgt tgcgctcgga tggctcttgg tcgggatcgg tcgtcagaag
    46921 gaccgtatcg tgatgcacaa cggcgggacg ggcggctacc ggagcttcgc cggctgggct
    46981 ccggacggtc gccacggcgt cgtggtgctg tcggcgaacg tccgcagcgt ggacgggatc
    47041 gcatcgaggc tcctgctcga cctcgaagga acttccgggt ctccctagcc ccggccgacg
    47101 aagaagcaca tcaccgcgag cacgagggcg agcggtgccc agaggtatcg ctcccaggga
    47161 ctcgaggatg cggcgttcat cacggccccg acggcgagca cgacgacgag gatccacgtc
    47221 ccccaccgcg cgaccaccgt cgggaaggaa ggtcgcgccg gccccggagc gggcgagcac
    47281 gaccgccgcg gcgaacgacc agaacacgac cgcgatcccg ctcgcgaccc gcaggcccgg
    47341 tgggagatgg gttcgcgtcc ctccccaggc cgcccgcccc agcggtgcgc cgagcgcgag
    47401 cgcggcctga aacgaggcga tcgcgacaca tccgagacgg cgagcgtcct cacgggctcc
    47461 ggacaacgga gatcccgtcg gattcgatca cccacctatc ttggcgcctg gcgaacggat
    47521 cgagcggtcc ggttcccgcg tccgtccggt cgatagtctc gggcgacacg aaggagacga
    47581 gcgatgagga agctgatcgt gaccaccttc ctgaccttcg acggggtgat gcaggccccg
    47641 ggcggacccg aggaggacga cagcggcggc ttcgcgcacg gcggctggtc ggtgaactac
    47701 tgggacgaca tgatgggcga ggccaagacc gaggagatga gcgggccgtt cgacctcctg
    47761 ctcggacgcc ggacctacga gatcttcgcc gggtactggc cgaacgcccc ggaggaggcc
    47821 ggcggcaagg gcttcaacga cgcaacgaag tacgtctgct cgcggagccg tccctcgctg
    47881 gaatggaccc ccgcgatgct gatggaggga gaggcggccg agggcgtcgc ggcgctcagg
    47941 gaggaagacg acgggccgga gctgcaggtg cacggcagcg ggaacctcgc ccagacgctg
    48001 atccaccacg gcctcgtcga cgagtaccac ctgtgggtct tccccgtcgt gctcgggaag
    48061 ggcaagcgcc tgttcgccga cggcacgatc cccgccgggc tgcggctcgt cgattcgaag
    48121 atctccacca ccggggtctt catgggcacc tacgagccgg ccggcgaggt cgttacggga
    48181 tcgttcggct gatcgatcgc ccagaggcct gagccttcgc gtcgactatc ccgcggactg
    48241 ggctagctgg tcggcgatct cccggaacgt cccgagccac gtgtcgttgt cgatgttcca
    48301 gtcgctgccg ttccggacga tgaccgcgtc cgcgtcggga gccacatacg ggtactggcc
    48361 gaggttcccg agcccggaga agcggcccgg gcgttcgaca tcgatccacc aggagtattg
    48421 gtgtccgccg ccggatcggt gcccgtgtcc gccgccgtgg cgacgcggac ccactccttc
    48481 gagacgatcc gtgtgccgtt ccactccccg tcgtggagga acagcagccc gaagcgggcg
    48541 tagtcgacgg gcgcggcgtt caacccgctc tccaacttct cgaagccgga actctccgag
    48601 tcgagtaacc aggtcgcatc actttccgcg ccgatcggct gccacaacct ggtggccatg
    48661 taccgcgaga ccgacatccg cgtggctcgc tcgaggacca tccccaaaga ggagcgggtt
    48721 gtagttgttg tagtgccacg tcaccctggg cggttccacg atctgcgatc cgttgatgcc
    48781 tccgtctcgg aggtccacgc cgtaatacgt gaccacgtcg tcgccccagg gaaccgggat
    48841 gtcgggttcc tcgtaccgga gccccgagga catggtgagc aggtcgcgga gcgtgatgcg
    48901 tccgaaccgt tcgtcgcgct ccgcgagctc gggggacata gtcggtgacc ggagcgttga
    48961 cgtcgccgat cagtccctcg tcgatcgcga tgccgaccag cgtcgacaag aacgacttcg
    49021 cgaccgagaa cgacgtctgc aaggtcgcac ggtccgaccc gccgaagtac cgctcgtaca
    49081 cgagacggtc gcggtggacc accacgaacg cgagcgtctc gttccgacgg aggaacgagt
    49141 cgaagtccgc gcccgcgcct tcgccgtccg ggatcgctcc ggtgagatcg atctcgggat
    49201 cgtcgggcaa cgggctcacc tcgtcgcctg ccgcgatcac gcgggacggg aaccgatgct
    49261 ggtcgccgac gtcggtgtcc cgccagacca tcgcccgggc ggtcgtggac gagtccacgg
    49321 ccgcccacgc ccacgcgtag accacccacc agcagaacga gcgcgcctgc ggcgatcgcg
    49381 agccgccggg gccatcgacg acgagatcgt tcgctcatcg tctgacccta cgttcgtcgg
    49441 ggctcgccgg aaccttggtg ttgcggctcc tcgctcgcgc ctcccgaagt cggcggtcca
    49501 cctcgacaca gcctgggcac cgatcttcga tcggcgcgat cggtccgatc cggtccaaga
    49561 tgggtcccgt ccatgcgcga cagctccgat gctgcccgga acgaccgtcc cacacgggat
    49621 cgcgacgggt acctgccgat ccgtggatac gccgcgatcg gcgacggacg aaccgtggcg
    49681 ctcgtggccc tggacggctc gatcgactgg ctcgccttgc cgaacctcga ttcgccgtcc
    49741 gtgttcggag cactcctcga cgcgagccgc ggcggtcgct tctcgctcga gcccgctctc
    49801 ccgtacgaga cgacgaggcg ctacgttccc cacacgaacg tgctcgagac cacgttcacg
    49861 acggaggtcg ggaccgtccg cgtgaccgac gcgttgttgc tgagcgatcc cggcctcgct
    49921 ccgctccggg agctggtcag ggtcgtggaa ggaattgacg ggatggtccc gatggggtgg
    49981 tccgtcgagc ctcgcttcgg ctacgcgtcc gcgcgcacgt cgatcgccgt gcgggacgga
    50041 gtgcccgtcg cgagctcggg cccggacgcg ctcgggatcg cgtcgtacgg gctgggacgg
    50101 ccggacatcg ccgatggtcg gatcaccgga cgcgccgagg tccgggccgg ctcgacgggg
    50161 acgctcgtcc tgtccgccgc cagccaggag cccgtggtcg tcccgagccg ggacgaggtg
    50221 ctcgatcgac tcgaccgaac ccgagacgcc tggagcggct ggacggcgga gcgcccctac
    50281 gcgggaccgt tccgcgacgc ggtggtccgg agcgggctcg cgctccggct ccttgtcttc
    50341 tccccgtcgg gagcgatcgc cggcgccgcg acgacgtcgc tccccgagca gatcggcggc
    50401 gtccggaact gggactatcg cttctcgtgg gtccgcgacg cggccgcctc gctcgaggcg
    50461 ctgatcgccc tggactgtcc cgtcgaggcc gaggcgtact tctggtggct gatgaacgcc
    50521 tcgcagctgt cgaggcccaa cctccgggtg ctctatcgct tggacggcgg cacccaggcg
    50581 cgggagcgga cgctcgacct cgccggctac cgacgttcgc ggcccgtgcg agccggcaac
    50641 gcggcgatcg accagctgca gctcgacatc tacggcgacc tgctccattg ctcgtggctc
    50701 tacgcggaca ccgtcggcga gctcgaccgg gacctcggca agcggctcgc ccggatcgcg
    50761 gatctcgtgt gccggatctg gcggacgcct gactccggga tctgggaggt ccggagcgag
    50821 ccccggcact acacccaatc gaagatgatg tgctgggtgg cgctcgaccg agcgatcgcg
    50881 ctggcgggct cgtctcgggt gccgtccgat cgtgtccacc gatggacgcg cgaacgggac
    50941 gcgatcgagg cgttcgtgga ggagcgatgc tggtcggagc ggctacacag ctacgtccgc
    51001 tccgacggct cggaggacct cgacgcgagc gtgctgcacg cctcgatcct cgggtacggg
    51061 gagcccgccg gtgaacggat ggtcggcacg ctggcggcga tcgatcggca tctccgggac
    51121 ggcccgctcg tgatgcggta tgtcgtggac gacggcctgc cgggcgagga gggcgcgttc
    51181 ctcgcgtgtt ccttctggct cgtggacgcc tgggcacgtg ccgggcgggt cgatgaggcc
    51241 gagcggatga tggagcgggt gatcgcgatg gccaacgacg tggggctcta cgccgaagag
    51301 gtcgatcccg gggacgggag cttcctcggg aacttcccgc aggcgctgcc gcacctggcg
    51361 ctgatcggcg ctgcgacggc catcgaggaa gcccgccggg atcggacgtg agcgtcctcg
    51421 gtgccatggc gggaggtgtg ctggggacct tcgtcctcac gaccgtgctc aggaccgcga
    51481 gcgagctcgg gctcacgcgc gtggacctcc cgttcctgct cggaaccgcc gtcaccaggg
    51541 atcgggggcg ggcgaaggcg atcggctacg tcgcgcactt cggcttcgga ttgctcttct
    51601 cgctggccta cgccgcgttc ttcctcgtcg tcgacgcgag cgggtggctc ctgggggcgg
    51661 tcctcggtct cgcgcacggg ctcttcgcgg ggacggcgct cgtgaacgtc ctccttccgg
    51721 tcgtgcaccc tcggatgggc aagccctgga ccgacgcgcg atcgtccccg ctgatcgagt
    51781 cgcccgggtt cctgatgctg aactacggcg ccggcacacc agtcgtcagc ctggtcgcgc
    51841 acatcctcta cggagcgatc gtcggcggcc tcgcggcgtg gtgacggctc ccggccgatg
    51901 tggggccagt caacccggcg gcgagaggcc gtaccggagg aacaggaagt cctcggagcg
    51961 gcggatcgag agcagacgcg ctcccatccc ccgctcgagg aggtcggtct cgtccgtcag
    52021 gggagtccgc tccgggccac ctccgatcag cttcggcgag atcgtcagga acagctgatc
    52081 gacggccgaa gcctccagca tcgcgcccat caaccgggga ccaccctcgg tgaggatccg
    52141 ctggtatccg cgctcgcgga gacggtcgat cgcgaccgcg ccgtcgacgc gatccgcgtc
    52201 cccgacgtcg atcacctccc cgacccgggc cctgccctcc gcgagcgatc gagcaccggc
    52261 cgccgtcgtg acgacgatcg cgtccgacag agcgggatgc tccgcgggga gatctccgct
    52321 cgcgctcacg acgacgaacg tcggcgattc gctcgccgag atccccgctc tcaaccggga
    52381 gaagctctcg gcgcccggcg ggaacgcctt ctcggcggtc cagggaccgt cgtgctcccg
    52441 gagcgtcccg gcgccgacca cgacggcgtc ggccacggcg cgcaggagcg ccatcatgaa
    52501 tcggtcggtc gaggcgcccc cgctgatcgc ggccgaagac atcgcgacgc cgccgatcgc
    52561 ggcgacgccg tcgatcgacg acacgaagtt cgcatagacc acccgttcgg caagccggaa
    52621 cgatcccccg tacgcggatg ccacctcgtc ggggaccggc acgtcgggag cgtcgtcgga
    52681 ctcgaagacg gcctccaggc ggacgtcgag cgcggtcact cgcgctgcgc cacggtcagg
    52741 aactcgcggc gcatctcggg atcgcgctcg tagttgccac gccagaacga ggtggaggtc
    52801 ctcgaacgct cctcgcggac gccccgcatc tgggtgcaga ggtgcgcggc ctggagatgc
    52861 acggcgaccc cgtgcggatc catcacgtcg ttcaggtgat cggcgatctg ctggccgagg
    52921 cgttcctgca ccgtgaaacg gcgtgcgtac acccggacca acctcgtcat cttcgagatc
    52981 ccgatgatct gctcgtgcgc cacgtatccc acgtgcgcca cgccgtagaa ggggaaggcg
    53041 tggtgctcgc agagcgcgaa gaacgggatc ggcccctcga tcacctggct gatcgagcag
    53101 tcgggccccc cacgacactc ggtggggaac gcggtcagga gcttcggatc gccctcgtag
    53161 cccgtggtcg cctcgtacat cgctcgaacg aaccggcgcg gcgtgttgtg ggtcccctcg
    53221 gtgtcgaggt ccatgccgaa ggcggtgaac atctccgcga cggcgtcctc gaagcgccgc
    53281 cactgctcgt cggtaacctc ccgaggtcgc acgcgctccc acgacgtggt ctcctccggg
    53341 tcgctcatcg cccagcgctc tccgacgtcc gcgggtctcg cggtcgagtg ccgcccatcc
    53401 gggatctctc gatcggtcct catcctcggc ctccgcctcg cttcatcggt cggctccctc
    53461 cacccaccgt acggtcccgc cgggcgtttg gcctgtctcg acaggcagcc ggtctcgagg
    53521 agctcagact atgaaggctg acccggctgt ctcggtccga tccggtacgg ccgcatcatc
    53581 tcgttgtcct cgtgctcgac gatgtggcaa tgccagacga actgcccggc gttcgtgaag
    53641 cgcatccgca tcctggtcac ctgtcccggg taggcgatca cggtgtcctt ccacccgtcc
    53701 tcccacggct cgggtggact cggctccgag gttccgtcca cccggacggt cccgctctcc
    53761 tcgtcgatga cgacgtcctg ccggtcgacg acctggaaga gcacctcatg gatgtgcatg
    53821 ggatgagcgt cgccggtggc gttgtagaac tcccagactt ccgtgtcgcc gacctgtggg
    53881 ttctcggtca cggcgtcgtc ccatcccagg gccgtcatcg atgcggtctc gatgttcggg
    53941 tcaccctcga tcgtcccgag gtgtgcctcg accggcgctt cctcgaagtc ttccggcggt
    54001 tcgtcgaact ccgacatctc ctccagcagc gccagcggtc gggtcgttcc tccggtgagg
    54061 cgagtgatcg ccgggagcac catgaaccgc ggcggcgtcg tcgggtcagg tgccacggcg
    54121 ggcacgaccc ggaactgcag cacctgaccg gtggacgccg ggtcggcggg ctcgaagtcc
    54181 acccctggga tcccgcctcc gaacggctcg tccggaccga ggttcccgag gacgtgctcg
    54241 cccaccggca ccttcgtgaa gtcgacaacg acgtccgccc gctccgccag ccccatcagc
    54301 agccggttgc cgttgacctc ggtgaggttc accggcgcgg cgaggaaccc accctcgttg
    54361 ccgatctgcc acacctcgac gccggggatc tcgctgaagt cgaggatcag gaaccgcgac
    54421 tggcacccgt tcaggaatcg gaaccggtag cggcgccgct ccaccgtctg ggaaggccac
    54481 gtgttgccgt tgaccatgat cgtgttcccg aagaactccg ggttccagat gggcgagacg
    54541 tccgtctccg ggatgtacgg cccggtgatg ccgtcgaaga aggcacgcgt gtccgggtag
    54601 aagagggaac cgttcgcgtc gaacgaccga tcctggatcg cgatcgggat ctcgaagtag
    54661 gtcttgttgg acgggaactg gtcgttgggt ttcggggcgg gaccggggag cacggcgggc
    54721 gtgccggagc ggctgtcgag cacggcgtcg tccccatcgg gcccgccgcg gatcaggaag
    54781 aagccggccg gcccggcgta gacgttgagg cgcgtcatgc cgagcgcgtg gtcgtggtac
    54841 cagatcgtcg aggcgcgctg ggagttcggg tactggaacg tcgcggagcc gggcccccag
    54901 gcgacgccgt acttcgatgc cgccttgttc gcgaagaagt cgtaccgggt gccctcggtc
    54961 gcgtagtccc cagggatgtc gttcgcatcg ggcaggaacc acgcctcggc gtagccgtcg
    55021 ctctcgtcgc cgacgctcgc cgagccgtgc acgtgggtca cgatgggcac gggcccggtg
    55081 tagggaccgg gcgtcgtctc gaacgtgggc cgcatgtcac gcccctcggt ccctccgggc
    55141 gggttcgccc agtggagcgt ggggtcgacc gggagcaggt gcggcaggaa gtcgccgttc
    55201 gcatcgacga ggtcgttgat ccacgtgatc cggaccgggc gcttccacct ggcctcgatc
    55261 gtgagcgacg gcgcgttgtg gacgagcagc ccccgcttgc tcgccgaacg cacggctcca
    55321 tacccccaga ccgtcgtcgc cggcatcgtc gcgggaagga tctgttggga gaactgcttc
    55381 atcgagatct cgtaggagtc gatcggttgg cttccgcgct gcgtgatcac accggctcgt
    55441 ggcatcacgg gcgggatcag caggggtgtg acgaaccggg ggatgacgcc gggctccaat
    55501 gagcctcccg ggatggaggc gacggcgcgt tggatccacc ccgactgcgt ggccacgtac
    55561 caagcgacgg tcgagccgcc cgtgaccttc aggaagtctc gacgcgtcag caaaggctac
    55621 ccctcctatc cggcgccgat ccgggcgcct ctgaacgtga catcagggta ggcctcatcg
    55681 tctcgaggac acaagccgtt cggtcgcctc gtgagtccca tccgaagcgt gtggaacacg
    55741 gcctcgcgca ccgctggagg gctaccggct gcggtatcgc gccacccgaa cctgatccag
    55801 ggagcggcgc ggagcgcgcc gctcccggtt cctcgatggt tctcagcggg cgtgtgcccc
    55861 cgtcccgaac agtccggcgt cgtgcatcgt ctcgatcgcc cacacaacga cgatcgcgcc
    55921 gacgaaggtg gcgagcagga gttcgctccc ctcggcaccg acgagcaccg ccgcgatccc
    55981 gagcccgagc gttgccacgc cgaggaccaa catcatcttc ccgaacgcgg accgcagtcc
    56041 gagtccccag gccacgaagc cgaggagcag cccgacgacc ccgaccccgg ccatcccacg
    56101 ggggtcctgg atgaacggca tctcgcccca gatcaggtag ccgacgtacg ggatcaccac
    56161 ggcggcgatc agcagcgtgg cgatcagatc cttcctggtg agcttcatcg ttcccacccc
    56221 tttccgttca ccaccatggt gcacaccggt acgccagctg atggaggcct gccgtcccgg
    56281 catcgacagg acttcccgtc agttcgcggt tcctccgatc gttctcgacg tccggggaac
    56341 gtccgggatg tcagggcttc cgtgcggtgg ctcggcccca ggcgaacgcg gcgaacgagt
    56401 cgggttcctc ggcccaccgg atccaagcct gctccagcgc atcgatctca tcccggtcgg
    56461 cgagtccccg ttcggtcagt ccccacacga accactcgtc gcggcaatcg gcggcgcgac
    56521 cgagcccgaa cgttcgaacg agttcttcgg tcccgtagct gaagtacgtg gtggatgcct
    56581 cgacctgcgc gaaccccgcc gccaggagca aggcgcggag ctcccggccg cgggacgggt
    56641 ggacatcgcc ctgcgcttcc cacagcgcga gccggagctc gtagaagcga cgcagcgaga
    56701 ccacgtccgc gccgtggagg atcagccctc cgtactcgat cgacgacgct ccgatgacgc
    56761 cgccgggctt cagcgctcgt cggacctcgg cgagtcccgc gagcgggtca tcgagcgtct
    56821 ccagcatcga gaggagcgtg cagacgtcga tcgatccttc cgcgacgccc aggtcgtaga
    56881 tgctgccctc gcggaactcg acgttgccga tgccgtggcc ggcggcgtac gcgcgcgcat
    56941 ccgcgaactc cgcgtcgtcg acgtcgatcc cggtcacgtg cccggcggcc tgcgcgatgc
    57001 ccagggtgat cgaacccggt ccgcagccca catcgaggac atgatcacct cggcggatcg
    57061 cggggagcac gaaatccgcg taccgcgcgg cgctccggtc tcgcatcagg tcctcgaacc
    57121 ccacgaccgt gcgctctcag tggtcggatg cgtgcgactt cgcgacgtcg ccgatgatct
    57181 cggccagttc cttcggacgc gaccacatcg gccagtggct cgtcggcagg tcgacccagg
    57241 tgaggtctcg cacctcgtgg agaccggcga gccaggcgta gccctcgccc atcgcctcct
    57301 tgtactgctg ggacgtgtag cccgtgcaga tgatcgtgct cgggaggtcg agccgcgcgt
    57361 cgttggtcag ctcgacggtg tcgcggagga cgccgccagg ctccgggacc gcccgctgcc
    57421 ggaacgtctc gagctgcccc tcgctgagcc cgtcgaggtt ctcctccgcc gagagctcct
    57481 cccacgacag cggcttctcg tccccgtcga aatcggcatc gaggggcggg atccccgggg
    57541 cggtgtcgac gtagaccatc gccgcgatcg acccggggac ccgatcgctc gcgacgtagc
    57601 cggagaaacc ggtgccactg tgcacggcga gcacgacggg atcgcccgcc gccttgaccg
    57661 cctcggcgat cgcgtcgacg tggtgggaca ggccgatgga ggagcgatcc acgtgggccg
    57721 actcgaggcc ggggagcgtg agcgcggtga cgtcgtggcc ctcggcccgc aacaggccgg
    57781 cgacctcgtc ccacgcccac gcaccgagcc agaacccagg aacgaggacg atcgatggag
    57841 tcgtcatccc tcgaccctag gcgtggatcg ggacatccgt cagcctcggg agcagcgtgg
    57901 cctcactgcg cgccgcgagg aggggccgat ccgccgacct cgtgtgcggg ttccgttcga
    57961 cgaagctcgg ctaccagctc cggtgcatcg aggatgtcca cgcgatgctg aaggagcacg
    58021 cccactcagt cccagaggtc gatccgccgg agcccggcgt tgccgagcgc ctcgttcacc
    58081 tcagccgtgt gccagatgtc gtggctgaac acgcgctgga tcaccgagcc gcgggtgtac
    58141 acccatgacg cgtcccactc gggacgacgg atctcctcgc cgagcatctc gagggtccat
    58201 gtgtcgaggc agcgctcgac gatccggaag gtcgagtcga gggcaccggc gagccgatcg
    58261 gcgctccaga cgtgctcgag atcgtcgtcg cccgggcagt cgaacgccga gttcgggaac
    58321 ggcgtcgtct ccacgccagc ctcaccggcg aacacgcaga gccagaacac ccgctggcag
    58381 gcgaggtggc cgacggtcgc ccagaccggc caccgctccg gcgacggctg gatcgccagc
    58441 tgctccccgg tcatcgtggc gatcgcgtcg cggagccgcc gctggtactg cggccacatc
    58501 tggtacgcgg gccggatcat ggacctaggc gcccgcccca tcgtgccgat cgggtgccgg
    58561 ccggtaggcc agccgcacca cacccgagcc gaaagtcgtc gcgccgatca actggaggcg
    58621 gaccgggccg acaccatcga agatccgcgg tccggtcggc cagatcgacg ggctcaccca
    58681 gaaccagaac tcgtcgatca gaccctgggt gatgagggcg tcggccagct ctccggcgcc
    58741 gacggcgacc agggtcctgt cgtggcgctc cttgagcgcg gtcacgctct cggcaaggtc
    58801 gccctccagc agggtggcgt tccactccaa cggcgctcgc agactccggg acgccacgta
    58861 cttgggcatg ctgttcatct gatccgcgaa ccctttcagg ttcgggtcgt cggcgagtgt
    58921 cggccagacc gcggccagtc cctcgtaggt cttgcgcccc agcaccatcg cgtccgctgc
    58981 gaagaactgc tccagcgaga cctggttgct atcggggtcg agcaccagcc aaccatcggg
    59041 ctccggcacc ggcgcctcga acgcgccgtt gacggtcatg gagttgttga tgaccagtcg
    59101 tcccatcgag gcctccttgt cgtcgagcca acgttcagtc gtagcggcgg atcgagcgga
    59161 cgctgagcag gaccatcaag gcaccggcac cggccacgac agcgaaccct cgaccgatcg
    59221 ctccccactc cagatcttgc aggaccagtg aacgcatcgc ctccatgatg tacgtgaccg
    59281 ggttgtaggt ggcggcgatc tccatcggcc gggtgagcag cggacgcggg acgaagttcg
    59341 gcgtgaggaa cagcagcggg aagaagtcca accccgccga gttcgtcgcc gcggcgcccc
    59401 gtcttgagcg cgatcaactg catggatccg gagaacacga ccgcccatgc agcggtcagc
    59461 aggatcagga gggcgaaccc gagcgggccg ctggcgatcc ggatcccgaa cggaagggcg
    59521 atcgcgagcc gatcgtgtag tgcttgacga gctgttccgc ctcgatcgcg tacgtcatgg
    59581 gaccgcaccc tatcgagcga gaccgacgcc agccgtcgca ccggacggcc acgcgtctcg
    59641 agtacctagt cgtccggtag cgttccgaac gggtccttgg gtcgaggagg gacagatgat
    59701 gacggagcgg cgggtcggaa ctcgcgagga atggcaggcg gctcgcgacg agctgctgcg
    59761 cctcgagaag gagcacacgc ggatgggcga cgacctcgcc cggaggcgac gggaactccc
    59821 ttgggtgccc gtcgagaggg agtaccgctt cgacaccggg gacggcaccc gcacgctcgt
    59881 cgagctgttc gacggccgct cgcagctcct cgtctatcac ttcatgttcg gtcccagcta
    59941 cggggccggc tgcccgacgt gctcgtcgtc cgccgacgca gtcaacgccg tgctgtccca
    60001 cctccacgcg cgcgacgtga cgatgctcta cgtctcacga gcgccgatcg agcggctgct
    60061 cgcctacgag gaacggatgg gttggagctt cccgtgggtg tcgtgcggga acagcgattt
    60121 caacttcgac ctcgggttct cgtacacggc cgatcaggtg cgcgagttcg ccgggccgat
    60181 gctcgaaggg ggcgcgccat cgggcgtcga ggccaacgcg gccgccagcg ggaccgacat
    60241 cccgggctac ctcacggagg gaccgggctt cagcgcgttc atcgaggacg acggcgccgt
    60301 gtaccacacc tactcgaccc aagcacgggg cctcgagttc ctgatggggt actacccgat
    60361 cctcgatcga gcgccgaagg ggcgcgacga gggcgagggc cagccgtggc tccgccggca
    60421 cgatgagtac tagcggcatg acttcgatcc tgcgaccgaa ggcgttgcgc ccaggagatc
    60481 tcgtggcgat cgtggcgacg tccggggggc tggagaagga tgaggcgcca ctcttcgaac
    60541 gcggcgtggg cgtgctcgat cagatgggat tcgtcgtccg cgtatcgccg ctggtcgatc
    60601 tcgacaggag ttggtggtgg gcggccgctc cgccgaaaga ggtcgcggag gagttcaacc
    60661 gccacctccg agatccggat gtgcgtgcca tcttctcgct caccggcggc cggatgaccc
    60721 tgagctatct ggatctgatc gacgtcgagg cggtccgggc ggatcccaaa ccgttgctcg
    60781 ggatgagcga catctccgca ttgcacctgg ggctccatgc ccggacgggt ttggtcgcat
    60841 tgcacgccga cctcgtcacg ttcgggttcg gggagtggaa cgaggtcgac gacgctcgcc
    60901 gggaccagct cgctgacctc taccgctgcg tcctcaccga ggacacgccg ctcggtcctc
    60961 tcccagccgg gactcgatgg gagcgttggc ggtcgggccg cgcggaaggc ccgctgatcg
    61021 gcgggatgct caaccgtctg gtgcgcgtgc aggcgagctc ctccgcgctg gccccagagc
    61081 gattcgacgg cgcgatcctg ttctgggaag aggccttcac ttctacgtcg gtggtctgga
    61141 acgacctcca tgtgttgcga caggcaggag tgctggaccg gatctcgggc atggtcgtgg
    61201 gcaggccgtt cgaggtcgaa ccgacaccgc ccggacccga gtcgctccgc gacatcgtcc
    61261 tcgaggtcgt gggcgaccgt gacatccccg tgctgggcga cgtggacatc ggtcacgcag
    61321 ggccgaacct ccccatcccg ctcggcatcc gcgccgagat ggatgccgac gcggtgacgc
    61381 tgtcgttgct cgagcccgcc gtgacgatct ccggcgggtg acggaccgtc ggggagggcc
    61441 cagcgctgtg agcctcggct cagacgtgcg cgagcaccgc gtcgagcgga cgcggaggtc
    61501 tcgagagatc gagcgcgtca acaagcaggg gggcgtagtc gatcttgcgg ctcgagttcc
    61561 cgaggaagcg cctcagttgt tggtcgatcg gtcgtcggcg ccactcgggt tgcttctgga
    61621 gggtccggaa cggccccagc tcaccccgct cccccacgat gtccaccacc ctctcggcgc
    61681 cgaccgagcg gatgagttcg tcctcgaggt cctcgataca cgcgtagaac ccgaacgtct
    61741 ccagctcgag aggcgacgag cccgatgcga accccgcccg ctggagaccg cgccggacgt
    61801 cgggcgcctc ggccacgtcg tacaacccgg cgacggcgac gtcggaccca cgcggtccga
    61861 accgctccag gaaccgcccg atgttcttcg ctccgcccat agcgacgacg gcgacaccat
    61921 cgaccgcgag gacgcgtccc cgccgcgcgg cgagggcctc gagcgcacgc cggtcgctca
    61981 tcccttcgac gaggacgacg gcgcgcggga tcgtcgtcac cgggtgacgg cgtggagcag
    62041 gacgggttcg gacacgccct tcagctcgac cgcgccgacc ggctcgaacg cgatcccgtc
    62101 cgggaccacg cggtccacga tctcttgcgt gaccagcacc tggcccggcg ctgcctgctc
    62161 tccgatcctc gcggcgaggt tgaccgtccg gccgaagtag tcgcctccct ggaacaccac
    62221 ggggccggtg tcgaggccga catgcgcggg cggcagcccc gccgccgcta tggcgtcgac
    62281 catctccagc gcggcgacga cccctccgcc cggctcccgg aagtggaaca tcacgccgtc
    62341 gcccagccac ttcacgacct tgccgccgtg gcgctcggcg ggccgctgga cgagcggcgt
    62401 cagacgagcc gccagctccg cggccgcctc gtcgcctcgc tcctccgtca gtcgcgtgta
    62461 gcccgtgatg tccaggaagc agatcgccgg gggcctgacc agccggctgc ggagccccgt
    62521 gcgatcgagc gcgtcttcga tctcgccgat caggttctcc acccacgtgt gctcctgatg
    62581 cgcgtggtac atcgcgagca tcgcttgctc gaggagcggc gtggtctcct cgccgaaccc
    62641 gagcgtcgcc tcgtgcatct ccgcgtcggt catgccggag gccagggccg ggagcacgac
    62701 gtagcggtgc caccagtccg cctccacctc ggccatccgg cggagaccat cgccgtagac
    62761 ccggagcaac cgatccagga tggatgtggg gaatcccgcc gagacgagct tccggatcag
    62821 cgggacgacg cggagctcgt cctcgcgcat tcggtcgtcc ggctcgggca gcgcgaatcc
    62881 gatcgcctcg cggaggacgc ggaggagctc cagcgggatg ccggtgtccg cggcgacgtc
    62941 ctcgaacgaa cgtgggctca gcggggcgat caggtcgtac agcggcgcgt cgagccaccc
    63001 gaacgagagc tccccggcgt cgagcgcctc acggatcgcc tcgagcggca gtcccgtgcg
    63061 ttcgagcccc tggtacagcc gggtccgcct gacgtcgcct tccgagaagg cgccgtcatc
    63121 ggcgggagcg aggatgccga cgtgaaccag cctggtcacg tagtcgaggt cgacgccggc
    63181 tcgttcggcg acgtcccgat ccgagatccc gtccacgcac agaacctagc cgtggatgcg
    63241 gccggtgagc acggtagagg tagcgtccgt gtcagcggtt gcgcgggtgg tcgcgggagg
    63301 cccgcccgtt gccccgcttg tagtcgccgg tcacgcgcgc catcacctgc tgggatcgcc
    63361 cagttcgacg tcttccagga agcgagcggc ggcggaaccg cggagcgtga ccgcgttcct
    63421 cccacgatgc gtgatcacga cggtgtcccc gcgttgtgag aacgcgaacc cggcagggcc
    63481 gcccatgcag cgaggatgtc acgctcgatg cgacatcggc agcgaggcgg accgacgttg
    63541 agggtgctgg tctcgtggct tcgccggcgc tcgatcggct cctcgctcgc cgatctagac
    63601 gcacgtgcag agcacgtccg cctgctgaca ggcggcgcac cgcatcgtgc agtacctgtc
    63661 cgagccggcg tgcaggtggt gctcgtcatg ctcgccggcc acggatccac agatcgcgca
    63721 cttccacccg tctgtcgctc cgccgcactt cgggcagagc gtctgtgcat ccgccatctc
    63781 tcaatccccc ttcgggatcg gtactccatc tcggatgggc acggcgctcc gaccgtccgg
    63841 tcggagcgcc cgcccactca tcctcgctgc gacgcgttga cgcctcgcag ggctaggagc
    63901 gcccccgcaa cgaagatcgc cgtcgcgagg tagatcagcc ccaggtgccc gatcgtgtcg
    63961 aagttgttcg ggtagtgatc cggcagggcc accgccaatc cgagcaccgg agctgccacg
    64021 gcccccgtcc atgcccaccg caagcctttc cggacgccgt agacgacgag gaagagcacc
    64081 gcgacaccgg tggcggcgat gaaccccgcg acggcgatgt gcaggtgccc gatgtagtga
    64141 tacagcgacg ggctgaacgc ctggatctcg tccctcccga catccacctg gttcggaccg
    64201 atgccgagct cgaggaatgc gtcggtgaag ttctggatga agaagatcac cgcgtagccg
    64261 atgaagccga cggcggcgat ccccatcaac catgagcccc acttcagatc ccgctcgacg
    64321 gacgccgacc ccgcatcctc cactgagact gtcgccattc ctctcacctc cttctaagga
    64381 gataggacgc agcactcgac ttcgacattg cctgccgcac ccgatgcaat gaagtcacgc
    64441 ccagggacgt ctatcccttc gacggacacg tgcgacacac tcgaccgaga tgagttcgac
    64501 ggggacaacg gaccggacga aggaagcgtt ccaggagctc gtgcgtaagg agctcccggg
    64561 cttgtattcg ctggcacgcc ggctcgtgcg cgacggtgcg gaggatctgg tgcaggaatc
    64621 gttgctgcag ggctaccggg cgttcggcgg cttgaaggag gaggccgccg gcgggcggtg
    64681 gctgcggacg atcctcgtga acgtcttccg cgaccagctc cgcaagcggg cacgctcgat
    64741 cgatgaggtc gccgccggcg acctcgagga tttctccctc taccggacgc tgatcgacca
    64801 ggatccgctc ccgtactcgg actcgctcca caccgacttc ctggaggcgt tcggccgcga
    64861 ggacctccgc gaggtcctga tgcgcttgcc cgagatctac cgggcacccc tcgtgctccg
    64921 gtacatggac ggcttcgcga cgaaggagat cgctcgactg atgggaaccc cgctcgggac
    64981 cacgctcgct cggcttcacc gtgggcgaca tctcttcgag cggacgatgt gggcctacgc
    65041 ggaggagtcc aagctcctcc ggatgggaga agacgatggg tgagacgacg gccgcgatcc
    65101 cgtgctcgga ggcggtgcgg cagctctggg actacctgga tcattcgatc gcacccgagg
    65161 accaggagaa ggtggaacac cacctttcgt tctgtcgtcg ctgctgtggg gagctcgagt
    65221 tcgcgaagga gatccggcag ttcctggcgt ccggctcgtc cgaggagatc ccgaccgacg
    65281 tgaaggagcg cctggagcgc ttcgcggagg agctgtaggc cgtgacgaag gacctgatgt
    65341 accaggacga gatcaaggac gcggtccgcg acgcctacgg ctcgatcacg ggcggcgggg
    65401 gcgacgccgt cgccggcagg ctctacagcg aggaggagct cgcggagctg ccgtccggcg
    65461 cccgcgactg ggcgctcggc gtcggcaacc ccgtccggta cgcgttcctg caaccggacg
    65521 aggtcgtcct cgacatcgga tccggtggcg ggatcgacac gatcctcgca gcgcgccggg
    65581 tcggtccgag gggcaaagcg atcggcctgg acgtcgtcga cgcgatgatc gatagggcgg
    65641 gggcgaacgt cgccgaggcg ggcgtcgagg gctggacgga gttcctcctt ggcgagatgg
    65701 agcagatccc gctccccgac gcgtcggtcg acgtcgtgat ctcgaacggc gtgctgaacc
    65761 tgtcggcgcg caagagccgg gcgctcgccg agatcttccg cgtcctgaag ccggggggcc
    65821 ggatctcgat cgccgacctc accgtcgagg gcgagctccc gcccgagatc gcgaacgata
    65881 tgagcgcctg ggccgggtga gtggccggcg cgctggcgga gcgtgtcttc gccgagaagc
    65941 tcgggagagc ggggttcgtg gacgtgtggg tcggcggcca ccggccacag agcatcgacg
    66001 aggcgtcgct gtacccgctg ttcaccgatc acgtgatcga gctgatgcgg gcatcgttgc
    66061 ctccggaaca gcacgaccac gtcgcgacct cggtgatcgt gaaggcgcac aagccggagg
    66121 caggctccct gggatgacga cccgcaccga ccccgaaggg aacgaggccg gcatcttcga
    66181 tcgaggcctg tgaagcgctc gcatcccccg agagccaggt ggccaaacgt cggtagcggc
    66241 gaggacggca ggcgctcgat cgcagcacgg atctttccgg gatcagctgg cagctcggtg
    66301 gcatcgtttc ggcattgaca tgatgccaaa gtggtgccat catggcgcca tgaacatcga
    66361 cccgtacgtc gaccggatcc acacccagct cgatgccgcc gccgaagcgg gcggcgagga
    66421 cgccctcgtg ctcgccaggc ggttggcggc gcctctggac gccgcgatcc gcctcgccgt
    66481 ccaggaggcg ctggccgcgg ccgccgagga gatcacggtc gagctcgcgc ccggctcggt
    66541 ggaggtacgg cttcgcggac gtgagccgca gttcgtggtg acgatgcccg cgccacccgc
    66601 tgctgccgac gaacacgcgg gcgacgacga cctggcgctg agcgcgatcg ctccgcaggg
    66661 cggcgagggg gaggaccagc cggtcgcgcg ggtcaacgtc cggatgcccg agcacctgaa
    66721 ggccagggtc gaccgtgcgg ccgatgccga gggcctgtcc atcaacgcgt ggctcgtccg
    66781 cgcggccgcc tcgcgcctcg agcgcagcga gagcggccgc cgccccgagc gccgcgcacc
    66841 ctccggagcc cagcgctaca cgggttgggt ccgctgaccg agaggagaac gccatgccca
    66901 ccttccacac cccaggaccg atcgccgtga cgttggagct cggtgtcggc gacgtccgga
    66961 tcgacgcgac cgatcggacg gatacgaccg tcgaggtccg gccgagcgac ccgaccgaga
    67021 cgagtgacgt ctccgcggcc gagcagacac gtgtcgagta ctcgaacggt cacctgctcg
    67081 tgaaggcgcc gagcagctgg cgccggtggg cctcgcgcaa gggcgcgtcg atcgacgtcg
    67141 agatcggcct ccccgtcggg tcccggctcg acgtggaggc cggcgtcttg acgctccgcg
    67201 cgagcggtcc catcggggag acccgcggct cgctcggcgt cggggacatc cacctcgacg
    67261 ccaccgggcc gatcgagctg aagtctggcg cgggtgacat ctccgtggag agggccgccg
    67321 gcaaggctgt gatcacggcc ggctccgggg gtgtgcggct ccgcaccgtc gacggccccg
    67381 ccgtggtgaa gaacggcaac ggcgccacgt ggatcggcga ggtgaccgga gaggcccgcg
    67441 tgagcgcggc caacggtgac atctcgatcg acgtcgcgcg cgccggcgtc ctcgcgaaga
    67501 ccgccaacgg cggcgttcgc ctcggcgagg tctccatcgg ctcggccgtc gcggagaccg
    67561 cgttcggcga cctcgacgtc ggggtgcgcg acggcgtcgc cgcgtggctc gacctccaca
    67621 cgcggttcgg gcacgtacgg aacgacctgg acgcgaccgc ggctccgggg aacgacgagg
    67681 gcagcgtcga cgtccgcgcc agcacgtcct acggcgacat ctcgatccac cggtcgttcg
    67741 ccggcgcggg gagggatgcc tcgtgaccgc caccacgtcc gcgccggccc tcgatgtcac
    67801 cggcctgcgc aagaccttcg gggagacagt ggcgctcgac ggggtcgact tccacgtggc
    67861 cgccggcacc gtcttcgcgc tgctcggtcc gaacggcgcc gggaagacca cggcggtgca
    67921 catcctctcc acgctcctgt cggccgacgc cggcgacgtc cgcgtcggcg gccacgaccc
    67981 ggcgcgagac ccggacgccg tccgccgcgt gatcggcgtg accgggcaat tctccgcggt
    68041 ggacgggctg ctcaccgccg aggagaacct ccagctgatg gccgacctgc accacctggc
    68101 tcgtgaggat gcccgaaggc ggatcgacga gctcctccag cggctcgacc tcgccgacga
    68161 agcggccaga ccggtcgtga cctactcggg tggcatgcgg cgccggctcg atctcgcgat
    68221 gaccctgatc ggctcgccgc ggatcatctt cctcgacgag ccgaccaccg gcctcgatcc
    68281 gcggagccgt cggacggtct ggcgcctcgt ccgcgagctc gtcgacgggg gcgtgaccgt
    68341 cttcctcacg acgcactacc tggaggaggc cgatcgcttg gccgaccgcg ttgcggtgct
    68401 caaccgcggc cggatcatcg ccgagggcac acccgacgag ctcaagcggc tgatccccgg
    68461 cggctcgatc cgtttgcact tcgccgacga tcggcagctc accgccgcgg ctcggatcct
    68521 cgctcgggcc acgatcgatg aggacgccct ggcgctcgag gtccccagcg acgcgagctc
    68581 gcggtcactg cgggacgtgc tcgaccgact cgatcgggcc acgatcgacg tggaacgcgt
    68641 ttcgatgcac acgcccgacc tcgacgaggt gttcctggcc ctcaccggtg aggaacccgg
    68701 ggacgacggg cgagagaagg aaggagtcgg atcacgatga acgcgaacac ctacgtcctg
    68761 cgcgactccg ccacgatgct gcggcgcaac ttccggcaca tgctgcgcta cccgtcggtg
    68821 tccttggtcg tggtcgcgat ccccgtgatc atcctgctgc tgttcgtgta cgtcttcggc
    68881 gacaccctcg gcgacggtct cggcgtcgcg tcgggtggcc ggggcgccta cgtgaactac
    68941 gtgacgcccg ggatcctgtt gatggcgatc gccggcggat cgcagagcac cgcgatctcc
    69001 gtcgcgatgg acatgaacga aggcatcatc gcccggttcc ggacgatggg gatctcccga
    69061 tcgtcggtgc tgaccgggca cgtgctcggc agcacgatcc agacgatggt gggcctggtg
    69121 gtcgtggtcg gcgtggcggt actggtcggg ttccggcccg acgccggccc gctcgagtgg
    69181 ctggccgccg tggggctgct ggggatgatc acgctcgcga tcacatggtt gtcgctggcg
    69241 ctcgggctct tccccaagag cgtcgaggcc gcgagcaacc tcccgatgcc cctgatcctt
    69301 ctgccgttca tgggcagcgg gttcgtcccg acggactcga tgccgaccgc gatccgatgg
    69361 ttcgccgagt atcagccgtt caccccgttc atcgagacgc tccgcggact cctcatgggg
    69421 acgccgatcg ggaacagcgc cctgttcgcg gtcggatggt gcgtggtgct cacggtggtc
    69481 ggctaccgct gggcgatgcg gctgtacgag cgtccgcgct agttgggacg acgatcggac
    69541 gcacgagtcc acccccgggt cgtccacgtc ggcagcctgg gtcgctgaga ccgcctcgtg
    69601 ggggcggacg cctcgcgtag ggttgcggga gtcgctgcga gcagggttcg cgaaggaggt
    69661 cggggcgcga tgccgaaggt ccagggtctc tcgaaggcgg aggccaacta ccgcaaggcc
    69721 gagaacccga agttcacctg ccacgagtgc aaagttcatg ttcccgcggc tggcgatcgg
    69781 tggatgccgc tacgtgcgcg gcgtgatccg cgccgacgac atctgtgacg agttcgagcc
    69841 gcggagcggt gaggggaacg cgcgaaccta gatcggcgcg cccgatccga ccgaacgacg
    69901 accaacgaga ggggaaccgg gaccatgaag ctgacgacca ccacgatggt ctccgtcgac
    69961 ggagtgatgc aggggctcgg cgggccggac gaggaccgca ggggcgggtt cgagcgcggc
    70021 ggatgggtca cgccgtacgc cgacgacgag accatggggt tcctgaacga ggtctatggg
    70081 cgagccgacg cgttcctgtt cggccggcag acctacgaga tcttcgccag ctcctgggga
    70141 acgtgggacg atccgggcga cagcccgatc tggacggcgt tgaacacgcg gcccaagtac
    70201 gtcgcatcga ccacgatcac ggaaccgaag tgggcgaaca cgaccgtgct ctccggcgac
    70261 ctcgttgctg ccatccgcga gctgaaggcc aaagccggag gcgagctgga ggtgcacggc
    70321 agcggcgtcc tggtccggtg gctgctcggc aacgacctcg tcgacgagat caacctgatc
    70381 accgtccccg tggtcgtcgg ccagggcacg cggctgttcc ccgacacggg cccggacatc
    70441 gcgctcgagc tggtgggatc gcaggcgttc acccccagcg gagtgacgat ccaggtctac
    70501 cggcccgcag ggcgccctcg gtacgcaacg gccacgacct gaagcacgtt ggcgcgagga
    70561 acgtagattg cccggatgag cgacgtccgc gtgatcccgt tcctcgtctc tgaagtccgg
    70621 ctgcccgctc acgagcaaga gctgttgcga cgtgagtggt ggccgtcgta cgcccacgcc
    70681 gtcgtccatc gcgacggcat cttcctgttc gacaacgggg tcgggttcgg caacacagag
    70741 atcgacgcca cgttctcgcc acgggcgacg ccgctcgacg aggcgctcgg ccagtacggg
    70801 atctcgctcg cggacatcac cggagccgcg aactgtcatc tccacttcga ccacagcggt
    70861 cagaacggcc ggatccccac gaacgtgccc atcttcgtcc agcgggccga gtgggacatg
    70921 gtgcacgagc cggactacac ggtgcccgag tgggtcgacg tccctggcct ctcgtacgag
    70981 gtcgtgtcgg gcgaggtcga ggtcgggccc gggttacgtc ttatccagac accggggcac
    71041 acggccggcc accagtcact gttggtggac gcacctgacg gcacggtcgt cctggccggt
    71101 caggcggtgc agtcgcgagc ggagtgggag ggcggaaccg atccgtccgt gtccggagcg
    71161 tccgcagcgc ggcacccgaa gtacgccgac tccgtcgagc gcctccgttc gttcgatccc
    71221 gtccgtgtgc acttcgccca cgatccggcg gtgtgggaag gaggggagcg atgagcaagg
    71281 tcatcgtgat gaacagcgtc accctcgatg gggtgatgca gggcccgggc cgccccgacg
    71341 aggacactcg gggtgggttc acacgtggcg ggtgggcggt cgcgggcagc gacgagctca
    71401 tggtggcgaa gatgggagag cggatgggcg agcagcaggc gttcctgttc ggccggcgca
    71461 cgtacgagat ctgctcgcct cctggaacgc cctcggcggc ccgttcaagg acgcgctgaa
    71521 caacacccag aagtacgtcg cctcgaggaa cgcgacaacg agactggagt ggccgaattc
    71581 gacgctcctg tccggcgatg tcccggccga ggtggccgag ctgaagcaga catcgagtgc
    71641 caacctcgcg gtcatgggca gcggggagtt gatcgccgca ctgatggccg cagacctgat
    71701 cgacgagtac ctgctgatga tccacccgct cgtgctggga gccggccggc gactgttcgg
    71761 cgatggcgag cacgcgtcgc tgcggctcgt cgactggacg accaacacca agggcgtcct
    71821 gatcgcgacc tactggccgg ccgggacggg ttccgaacgg cccggttagc gttcctgcgg
    71881 ttccgcggcg ggctcgggcc caccggcacc gacctcctcc ggctcaccgc ccacgcggac
    71941 gacgaacgct tcgaggtccg aatcctcgcc ggtcagctcg cggtggatcg tgttggaagg
    72001 aacgatgaag aagtccccgg cgcttgccac gacgcgttcg ctcccgcccg ggccgaactc
    72061 gaccgtgatc gatcctcgga tcacgtagac gtgcgtgtcg ttcggggcgt gatggtgcca
    72121 accgctcacg tcgccggcgt cgttccggat ccagccggac cagccgaccc aacggccatc
    72181 gggatcgtgg aaatgccttc gctccgaacc gagcgccgcc ggcatctgtc cggtggggtg
    72241 aaggacgacc ggtcgtgatg cgctcgtcat ccaccaacca taggcgtcat gagcgcagcg
    72301 gtcgtcggtg atgaaggcct ccgtcgtaca ttcgcctgga tggcgagcta cgtgctggtt
    72361 cccggcgccg gcggcgacga tcggtattgg cgccgcctcg tgcctgcgct ccgcgaacgc
    72421 ggccacgacg cgatcgccgt cgcgctcccg gcggcagatg actcggccgg ctggtccgag
    72481 tacgcagacg cgatcgtgga tgccatcgac gaccggcccg aacccgtcct cgtcgcacag
    72541 tcgctgggcg ggttcagcgc tccgctcgca tgcgagcgga agcccgtcac acagatcgtg
    72601 ctcctgaacg cgatgatccc gaagcccggc gagaccggtg aggcgtggtg gtccaacacc
    72661 ggccagcgcg aggcccagct cgagtacctg tcgacgatcg gcgtgacgcc ggaggaggcg
    72721 tcggacgaca aggtcctgta cttccacgac gtcccgcccg acgtcgtcgc ggaggcggag
    72781 cgtcgtggag aacccgggca gtcgtggacc ccgatgacgc agcgatggcc gctggcgtca
    72841 tggccggcaa cccccacgcg cgtgcttgtc ggccgcgacg accggctgtt cccggaggag
    72901 ttccagcggc gggtcgcccg ggaacgcctc ggcatcgagg ccgagacgat cccgggcggc
    72961 cacctcgtgg cgctcgccga tcccgaggga ctcgcggaca ggctgatccg aaccgcaccg
    73021 tcgaggccct aggtcgttca tcacctgaag gcgccgcagt tcgacgagcc gatacgtttg
    73081 aggatcacga aggacgaccg gaggcaccgt cgccggcgga gtccacaccg gcggagatgg
    73141 ggggaagacg atggctgagg atcgttccta catcgacgtg aacacgcgtg agcgtgagcg
    73201 gctgcgtgcc ttcatcgagc gggtcgatga tgagttcctt gccgctcccg cgaacgagta
    73261 ctggaccgtc gccggcgtgt tgggccatat ggcctactgg gacagtcgtg tcttggtctt
    73321 cgccgagaag atcgatcgcg gggagccatg ggtgccttcg gatgaggaac ccgagggaga
    73381 ctggctcacc gactccaccc gtccgatcat cacgcgatcg agccgagacg cgctgcgcag
    73441 cttgcccttg agatcgccga ggaggtcgat cgccgcgtcg cggagctccc cctcgaccgg
    73501 atggcacctc gggaccccga cagcccgatc tggccggcgc gcggcgacca tcgaggagag
    73561 catctcgacg agctcgaggc cgctctgcgg gcgcggggct gacgcccaga ggcaagggac
    73621 ggcatctacg gcagctcgat cacgcgagcg caggggccgt ccgagggccg tcgcgccgag
    73681 acgacacgac cccagccgat agcacctcca tccatcgacg tctgtcgatg gatatgctag
    73741 cttccatcga cagacgtcga tggaaggacg gggacggtga ccgcgaccca gctcgagctt
    73801 caagatcgga cccccgcggc ggtctgtgcg ccgatcgggc gccccaagat gtcgatccac
    73861 caggcggagg ccaccgcggc cgtattcaag gcgctttccg atccggcccg ggttcgggtg
    73921 gtcaacctcc tcgcgagcgc agaagaaccg ctgtgcgtgt gtcacctgac cgagccgctc
    73981 aggctctcgc agcccaccgt gagcttccac atgaagaagc tgatggacgc gggcctgctc
    74041 gaccgcgagc agcgcggcgt ctgggcctac tactcgttgc gcgacgacgc cagacggcgt
    74101 ctgagcgtga tcctcgatct ggaaggagcc gaacgatgac cgagctccgc gagaccgttc
    74161 gcgagcgcta cgccgccgcc gccacatcgg tgaggcgcgg catggccgcc gacgacgtgg
    74221 tggaggaggc gtgctgcgac ccggccgacg gctacgggct gaagttctac gacatcaccg
    74281 agcgtggcgc cttgcctgac gaggcagtcc tcgccagcct cggctgcggc aaccccacgg
    74341 cggtcgccga cctcgccgaa ggcgagacgg tcctggacct cggctcgggc ggcgggatcg
    74401 acgtgttgct gtcgtcgcgg cgtgtgggcc cgacgggctt cgtctacggc ctcgacatga
    74461 ccgacgagat gctcgacctg gcgcgggaga acgcgcggaa ggccgacgcc acgaatgtcg
    74521 agttcctcag ggggttcatc gaggacatcc cgctcccgga tgcgagcgtg gacgtgatca
    74581 tctcgaactg cgtgatcaac ctgtcgatcg acaagccgcg cgtgatccgc gagatgttcc
    74641 gcacgctccg gccggggggt cggctcggga tcagcgacat cgttgctgcg aacgagctca
    74701 ctccggaaga gcgtgccgag cgtggcagct atgtcgggtg tatcgccggc gccctgtcat
    74761 tcgacgagta cgaggcggag cttcgcgccg ccggcttcat cggcatctcg atcgggccca
    74821 cgcacaaagc cgcagacgag atgcacagtg cgatcgtcag ggcgacgaag ccggcggtct
    74881 gagccggagg gaggaacggg tcatggccga gggaggcacg tcgctccttt cgaaggttga
    74941 cttcgaccgg accgtcccgg cgctcgcgcc gcacgcctag gtctggccgg tcgatcgccg
    75001 cgtcgcggtc cccctcgacc ggatggcacc tcgggacccc gataggatcc tcgacttgag
    75061 tgagcacatc gggagattga ccgacgtcat gccctggatt tggtcggggt acttgctgct
    75121 agctgagcaa gcgatggggg ctggcgcctt gagcctggga ggcgctagtt ggattggtcc
    75181 ggccgtgtcc tctggtgttc attcgaagac tgctctcacg tgctacgagt cgttccgtat
    75241 cccgcgcctg gaagccaacg ggctcggcga cgcactgatt cgccttggtc tcgactctgc
    75301 cgagccggcc gaggttgcgc tgttcgcagc tgaccactat gcggaactcc ttggcctcca
    75361 tgtacccaga cacgtggttg aggcactcgt aacatgagac ccggttcgcg ccgttagact
    75421 tcgtcgacct caacctgtcc ggatgaacgc atgtcacgag tgatcctgat cgcgtgggat
    75481 ggcgccgact ggcgcatcgt cgaccccctg ctggagcagg gcgccctgcc caacctccag
    75541 gccctcctcg acaagggtca gcgtgaggtc ctgcgctcca cggtccccac gcactcctgg
    75601 tcggcctggc cctcgttcct gaccggcgtc gaccccgacg accacggtgt ctacgacatc
    75661 ctcgagacga agccgggcac cctcaagcag tacccggtca ccctcaagca gtacccggtc
    75721 acctaccgct cgatcaaggc gcggacgttc ctcgacgacc tcacggccgc cggcaagggc
    75781 cagctcctgc tggacgtccc agcctgatcg ccgcctcacc ccgcacgcgc aggtaaaggt
    75841 tctgcttctc cacgcccact acctgtctga gtggacgatg tgcgggtgac ggaagcctcc
    75901 gagatcgagc gcgtctacag ggaggtcggc gaccgtttgt ggtgggcact gttggcgtac
    75961 accggtgacc gcgagatggc gagcgacgcg gcggccgagg cgttcgcgcg tgcgctcgcc
    76021 acgccggaga cgatccgtga gcccgccgcg tgggtgtggc gagtcgcgtt ccgggtggcg
    76081 acgacgctcc ttcgcgtccc gaaggcagcg agtgaccgcg aggcgagcta cgagatcgac
    76141 gaccgagccg cggacgtgat actggcgctg aagcagcttc cgccgaagca gcgcgccgcg
    76201 ttcgtcctct tctacctcga ggacaggccg gcgacggaga tcgccgagct gttggagatg
    76261 gcgccggcga ccgtctcggt gcacctccac cgcgcccgcc gcaagctccg cacgattctc
    76321 ggggacgacg atgcctgacc cgagtgaatg gatctcgcgt atcagggagg tcgagccgcc
    76381 agaactgtgg gaggaagcgg cggcacgtgc gagcagcgtc agcgaatctg atcgagctgc
    76441 cgttccggac ggacatccat cgagccaacc gacgaaccct cgtcgggctg ccctcgtcac
    76501 gggggttgcc gcgggcatcc tggcgctcgc catcgttgtc ctcgcgagcc gtacgggacc
    76561 ggccgagaag ggcgagaacc gagcgacccc gacgcccgag gtctcgtcgc tgtcggaggc
    76621 ccacgggctt ctcgagcgac tcctgggcga actgtggacc gcggaggcac ggctcgccga
    76681 gctccaaggc tcgctcgagg cggcgcacga cgaggtcatc gttctccagg atgagctcgg
    76741 accggatccg accgatgagc agcttcggga gatcgagatg cttcaggagc ggatccaggc
    76801 atggacgaga tcggttgcgg aggatcaagc ggtcgtctcg gagctgcgcg atcgcgtgga
    76861 ggaggctcgc gccgcgcgag cgcgactgat ccccccaccc ctcggcgggg tctatcccga
    76921 cgtcgcgacg gtcacgtgcg gcggcgacta cacaggtgga actcaactct ccacccccgt
    76981 tgtccggatg cagccggacg gcgttcactt ccgtgtgatt aacgggatct cgaacgagcg
    77041 cgtgttcctc gtcatccaac cggatggggc gcgggcgatc cctccagggg caacgctcga
    77101 ggtcgtgatc cctcctcgcc cttcccatga cgtcgaggtc acgtgcacct tcgactaccc
    77161 acgcgacagc tgggagcgtc cgacccatcc cctctggatc gagcgtccct caacgtgtgc
    77221 cgatgacgac gaacgaagga acgctgcgcc cgtcgatgga acccgggagg tgtcggtcta
    77281 cttctcgtgc gaggaaaacg cggagctgct gggcacggac ggtcagcccg tgtacatgtt
    77341 cgctcgctcg gtcccggcgg gatcgagtgg aacgtcagac gggcgcttcg agtctgcgct
    77401 tcgagcgtat ctcgcaggtc cgactccggg agaggccgag atgggctact tcaccccagc
    77461 ccccgcgtcg ctcggcgctg ccatcgtgga ggtgtcgatc tccgagggca cggcaaccgt
    77521 cgacttcgac gcctcggcgg agggtgacct cgggaacctc ggcacgtcca cagcatctct
    77581 gaccttcctc actgagcttc gagcggtggc cttccagttc ccggaggtgg acagcctcgc
    77641 tcttcgagtg gatggagact gcgacctgtt ctggcacatg ctcgagatgt cgtgccagac
    77701 gatccgccga gcggatacgt gtccgaccgc ccagcttgcc gtgtcgcgac ttccctggct
    77761 tccgctggac tccggggtgc ccgatccggc gctcctggaa gacgagctcg gcccggtgct
    77821 cgtctggttc gaagacgaag atcagcgctg ggcgggacga tacgtcgctc tgaaaaggtc
    77881 atccgagccg gtggtcggcc agggcctcag cgacttcccg acggcggaag tgcgggggga
    77941 gatcgggcat ctggtgtgga tcggggaccc gggagcgggc gaggtcgccc tcgtttggag
    78001 tgagggtgtt caaccatgca gctggttcag cctgagcatg tcgagcgtcg gcttgaccga
    78061 ggcggaggcc gagcgaacga tccggggact ggcggcgtac gtcggggagc ggtcccccta
    78121 atcgaagttg gcttcgcgcg ttccacgcac atcgcctcgc gtggttgaac atcggcgttg
    78181 ggtagactcg cccgaacttc gctgcgagag aggcttcgac gcatgtcacg agtgatcctg
    78241 atcgcgtggg atggcgccga ctggcgcatc ctcgaccccc tgttggagca gggcgccctg
    78301 cccaacctcc aggccctcct cgacaggggc cagcgcgagg tgctccgctc gacggttccc
    78361 acgcactcct ggtcggcctg gccctcgttc ctgaccggcg tcgaccccga tgaccacggc
    78421 gtctacgaca tcctcgagac gaagccgggc acccacaagc agtacccggt cacctcccac
    78481 tcgatcaagg cgcggacgtt cctcgacgac ctcacggccg ccggcaagaa ccagctcctg
    78541 ctcgacgtcc cgctcacgtt cccggcgccg gacatcaacg ggaacctcct cgccggcggg
    78601 gtcctgccga agggccgcac gttcacccac ccggccgacc tgcccgacaa gctcgccgag
    78661 cacggcctcg agtggccgat caacgggatg agctggacca cctaccggaa ccggcccgac
    78721 gcctacctcg acgaggcctt cgaggtcacc gcgaagcgga tcgcggtctc cgagtggctg
    78781 atggacaact cggactggga cctgatggcc tcggtgtggg tctcgatcga ccggacccag
    78841 cacgcgttgt ccaactacat cgcgcccgac catcccgact acacgaagaa caaggacacg
    78901 cggatcggga agaaggtcgc cgacatcttc cggcagaccg acgacgcgat cggccggttc
    78961 gtctcgcgcg cccgccccga cgacctgatc ctgttcatct ccgatcacgg cttccagtcg
    79021 tgcacccgca ccctgcatat ggaccatctc ctgaagcagt tcggctacct ggagttctcg
    79081 gcgtcgcagg cgctcttcgg ccccatgcag tggggtccga tgcggaaggt cgcgcgcaag
    79141 gtctacgaca tgctcggcct gcacgggaag gtgtcgctac cccagtcggt gaactggtcg
    79201 aagacgaagg ccttcacgac gatccgctcc accggcgagg gggtctctgt gaatctcgcc
    79261 gggcgggaga tggacgggac cgtggacccc ggcgactacg agagggtccg cgacgaggtg
    79321 atggaccggc tggggtcgtt cgtcgacccg aagaccggga agaagccgat caaagcgatc
    79381 cacaagcgcg aggagatctt caagggcgag ttcgccgacc gcgcgcccga catcatgttg
    79441 gtaccggctg aggggtactc gctcgcgcac gcgcgcaccg ggctcgagga cgccgactgg
    79501 gtgagcggcg accaccggct cgaggggacg atcgtggcgg tggggccgaa cgtgaaaccc
    79561 ttcgagtcgc agccggccct gatcgacatg gcgcccacgc tggtgacggc cctggacgca
    79621 ccgacggcgg tggagccgaa gggccggatc ctccatgaga tcgtcggctc ggagaaggcg
    79681 ctcaccgcga gagagtccac ggtgcagatc ccagggatgg gcgagtccgc ggtctccgac
    79741 aacgaagccg acgagatgga agagcatctc cgcggcctcg gctacatcga gtagcggccc
    79801 tggatggatc gtccgttccg ggtcggcgat ctcgtcgagt acgccgggcc ggagacacac
    79861 cacacgaacg cttgtccagg tgagtacggg cggctggtga cgcctgaggc cggtggggat
    79921 ccgccccctg aatgggtcgt cgacttcgac cgagtgatcg gcgtctactc cgcatccgac
    79981 ctgcgtccgg tggagttgga cttcgaagtc cgaagggtcg accccacagg tgtggaccga
    80041 gcgcggatcg ccgagttcct cgaggagcga gcgacggctc gcgtcgcgcg gctgggcgag
    80101 ctgatcaacg cgaccgagca agaagctctc atcgcggaga aggatggacg gatcgttggc
    80161 gtgctcacgt acatcgtcga aggcgatgct tgcgaagtcc tcacgctgca cgtcgccgag
    80221 cggtgggtag gtatcggatc cgctctcctc agggaggttg agcggctggc acgagctgcg
    80281 ggccgccgcc gggtctggct catcacgacg aacgacaacc tcgatgccct tcgcttctac
    80341 cagcggcgga acttcaatct ggccgcccta catgttgggg gcatcgtccg ggacaggaag
    80401 gtgaaacccg agattccggc gatcggtgag tacgggatct tcatccacga cgagctcgag
    80461 ctcgagaagc gccttcgtct ctgacccgcg gacgtagacc gaagccgacg agatgggagg
    80521 agcacctcag aggcctcggc tacatcgagt agcggcggag agtcgaccga cttcctcgag
    80581 gcaatccgac ggtgttccac ctctcggaac tcggttccac tagctggaac aggtcgaacc
    80641 cgagccccat cggcctggga tcgatctctt ctttacgttt ttacctttct ccggtacatt
    80701 ggccaggaaa tgtcaaaacg taaagcgcag gctatcccgg acctgatccg cctcctcgtc
    80761 gccgaccgcg agtccgtagc ggcgggcgag gtggccaagg ctgccggtgt cacccgccaa
    80821 gccgcgcact accacctcgt caggatggtc ggcgcgggcg agctccggcg ggtgggggcg
    80881 ggtcgaggat cgaggtactt cgccgacgcc gatctcgacc gatcgtatgc cttggaaggc
    80941 ctcgaggaag accgcgtctg ggatgaggtg gtccgggacg tgccgcagat ccaaggcgcc
    81001 ccggcgaacg tccgttcgat catgaggtac gccttcaccg agctgctgaa caacgcgatc
    81061 gatcactcgg gaggcacgga ggcatgggtg cgggtgtggg cccgcgatcc actcgcgatg
    81121 gagatcctcg acgatggggt cggcgcgttc cggcacgttc gcgaccggct cgcgctcccg
    81181 gacgacctcg ccgcgctcca gcagctgtcc aagggccgcg agacgacggc tcccgatcga
    81241 cactccggcg agggcatctt cttcacgtcg aaggccgtcg accggttcga gctcgacgcg
    81301 aatcgcctcc ggtggatcgt cgacaacgtc cgggacgacc aggcggtcgg ggacgctccg
    81361 gcccatcccg gcacgagggt ccgatccgag gtcgatccgg cgacgccgcg aacgctgcag
    81421 gaggtcttcg caccgtacgt cgacgaagag acgctccgct tcgagcggac gatcgttcag
    81481 ctgacgctct tcgagaccga gggacggttc gtgtcgcggg gggaggcgaa gcgcctcggg
    81541 acgaggctcg agcgtttccg cgaggccgtg atcgacttcg aggggatcga tgaggtcggc
    81601 ctcgcgttcg tcgacgagct cttccgcgtc tgggccgggc aacatccggg gactcgcctc
    81661 gtcccgatcg agatgtcgcc ggccgtggaa cgcgtcgtac gacgcgcgat cgcgacgtga
    81721 gcttgtcccc gcgagccctg gcgactatgc gacgccgggg cgaggcgcct gtaggcgccc
    81781 caccccggct cacgggtgca ggagtcagcg ccgctcgatg acgaagacgt ccgtcacgat
    81841 cctggtcagg atcgcggcgg cgatcggcgc gatcacggcc gccagcggca accgcgtcgg
    81901 gtcggtcccc gtgaccatct ccgggacgaa gatcgccacg agcgcgaccg ccgcccagat
    81961 cgcgaccatc aggagcgcgt aggtcagccc acgcgtcatc cccccgaggc cgcggatcat
    82021 ccctcgcacg acggagcccg tcgcaacgag gccggcgagc gacccgatca cgagcgcgag
    82081 cgggaagtgc tcctgcgtcg tgcccgtcac catgtcgggt gcgtagatcg ccgcgagagc
    82141 gaccgccgcc cagatcgcga tcagtgccag ggcgccgaag aggtatcccc cgctccgcgc
    82201 ctccgcaggc atcgccgcga ccggcttccg ctcggtcatc tccgggatgc gtcccttgac
    82261 ccctgcacgt ccgttcctcg tttcggtctg catgttcgtt cacctccagc tcgaccgtag
    82321 ccctgccccc ggtcggcgac gacgggcgga tgtcccgggt cgggagggtc cttcgctgcc
    82381 cgatccggcc ggtctggcga catcgcgcag ccgctcgtca catgggagac gggccgaatg
    82441 gcccgtctgg cctctcactg cgtcggtcac gctgtcggtg agaagggggt ggacgatgcg
    82501 gagacctcgg gccggctcct ggctcgtgat cgggctgctc gcagtgatgg tgctcgcgag
    82561 ttgcgccgag gtcgagcctc cggcgagccc gtctccggcg gcgtcggcgt ccgtgacctt
    82621 gtcaccatct gcgacaccct cgcctccggt catcccggcg tcttggaggc ggatcgcccc
    82681 gctgccgctc ggcggtagat gggacgaggc agcggtttgg gacggcgagg aggtgctcgt
    82741 cttcggccgg aagcaactcc agcgggagcc gtattgccga tacatcacct actcctacga
    82801 catcgccgac gacgcgtgga cccgcctgcc cggcgcgccc ggtccggccg gctgctacga
    82861 gggctccgac gaggtcgtct ggaccggcag cgaggcgctg ctctggggtg tcacgaacac
    82921 cgcctacgac cccgagacga acacgtggcg caagctccca cggccgcccg cgggcttcgg
    82981 cggtccgtcg atgatcgcgt ggaccggcac ggagatgatc ggatggggcg gcggctgctg
    83041 cggcggcgct gacgcgaccg gcgcggcgta ctcaccggcg acgaactcgt ggcgccgcat
    83101 gccgccctcc ccactgtctg cccgacatgc cgccggtgtg tggacgggca cggagctgat
    83161 cctcgcgggc ggccagggac cggacttcac gagcggtcca ccgacatatg cggacgcagc
    83221 ggcctacgat ccgatcgggc ggacgtggcg ttcgatcgct ccgatgccat caccgcgcta
    83281 cgcgagcgcc gcctggtggg acggcaccga ggcgttcttc ctcggcgggc gtcggttcgg
    83341 tacgaccgtc ggcggctgga cggagggtct gctccggcgc ggcgtcgcgt acgacccgag
    83401 caccgatcgc tggcggagga tcgccgcgat ggaggtgcca cgtcagggcg aggtcagcgt
    83461 ctggaccggc gaccagctcc tcgtgtgggg cgggatcggc gagagcgggt cgatcccgcc
    83521 gcatggtgag tcgtacgacc cactcaccga cacgtggacg ccccttcccc gttccccttt
    83581 gcgcgccagg accggtgcga tcaccgcttg gaccggatcc gagatgctga tctggggcgg
    83641 ggaggacgcg aggctctccg actggacggg aagcgaaccg ccatccggcg cctacccttc
    83701 cgatggagcc gccctcacac ctgcgggatg agcgtctgga acggcgagtc ggagcgggcg
    83761 gtcccgttct cctcgctcga gatctgacct ctcagcccac gtagtcgcgg gggtccaccg
    83821 agatcgagcc gtcggcgatg ccggaccacg ttctttaccg cgatgtcgta ccgcttccga
    83881 acgctggtga gccacaggtc ggcgaacgga ggcgcctgga agaactggtc gaagtcgacg
    83941 ccgatcagaa gggttccgcc cgcctcccgc gccgcctcgc cggcgccgag ccccgcctct
    84001 ccggcgaccg ggaagatgat gtcggcgccc tgcctgatca ggcgcctcgc gatgcggtgt
    84061 ccgccttcga cgtccccgaa cgcgccaaag tcgacctgcg agatgaacga gccgggtccc
    84121 ggacgccggg tcccaaccca cgagccggga cgtcggcgtc gtggtcctcg ttgtacctga
    84181 ggacgcctgc ggcgaaccca tccatgaagg ggaaggtgac ggctacttcg acgatccggt
    84241 cgcggcgtga cacgacgagg gcctcggcga tatcggagta gcccactcga gatctgagtc
    84301 gaacgggaac gccatctggg tgttctgccc atagacacgc cggatcgccg gatccacatt
    84361 ggtcacatca gatcggacgg aggaggctga gggatgcgtg accggcgatc gacaagcccc
    84421 gaggagccga aggtccagcc accgcccgcg cggtcagctg aggaggccgc gagtctggtc
    84481 cagcggctcg acgtctgggt ggccgagcgg gtgatcagcc gggcggacgc cgatgcgatc
    84541 gcagccttcg aatctggcgg gggcggtccc cgtcgcggag tcacacccct caccgaggcg
    84601 ctcgcctacc tcggtgccgc actcgcggtg gccgcgggag gaaccgtcgt aggcggcgag
    84661 tggggcgacc tcgcgacggc ggggcggatc gccgtccccg gaacgatctg gctcgtactg
    84721 ttcgccgccg gatcgttgat ccgtcgcgcg acgtcgccgc cgaaggtccg gctcgcgcgc
    84781 atcctgtggt tcctctcggc cgcttcgctg gcgtggaccg tgggatcggt ggtcgtggac
    84841 ggcttccgag ccgagagatg gcccctgtgg agcggcgcgg cggcggtgtt cttcgcgggg
    84901 gtgctctacc tcgcccggcc gagcaccctc cagcagcttg ccctggtcgg cgggttgatc
    84961 ctcctcaccg tgggtctcac ggaggattcg caggtggcca acgggatcgt gtattgggtg
    85021 gtcggatcgg cgtggatctt cctcggatgg cgtcgggtgc tcgttcagcc cggcgcggcc
    85081 tcaaccgtcg gatccctcct ggtgctggtc ggcgcgctcc tgatctcggt ccagcatgag
    85141 gagatcgggg cgtggctcag tgtgctgacc gcagccggtc tgatcggggt cagtgtcggg
    85201 atccgacaca cctccatgct cgtgctcggc gcgatcgggc tgttcttctc gacgttcggg
    85261 acgatccagc agtacttgga gggcaccacc ggcatcgcga tcggcctcct cgtggccggt
    85321 gtcgtcgtgc tcgcgctggc gctcatcgtg tcgcgcctca ccagcccgag tcggtggagt
    85381 atcgaccccg ggccagcttc ctgatcgacg tcctctcgga tccctctcag ggcatcgccc
    85441 gaggtggggc gatccggtcg ccggagaacg aggtgcggag gaagcgctgg ttgcgcttcc
    85501 tccgcagacc tccagctcac ggggtccgtg cgagggagcc gactcgcttc gccctccgag
    85561 gcacgtgcga gacgtgtcga tggacgttcc ggttctcggt cgacacgccg ctgccgtcga
    85621 gagccgcgta cagctgccac tgacccggga ggcctcggag ctcgtgcgac ccgcgatcct
    85681 cgaacagcag gtcggaccca gccgccacgt cacgcacggt gcgggtcacg agcacctcac
    85741 ccggtgccgc tcgtgcgacg acgcgggcgc ccgcgtggac cgcgatcccc cggaccttgc
    85801 caccatctcg gatgcactcg ccggtgtgga gcccggctcg aaccttcagg ccgagctcag
    85861 cgagcgagac cgcgacggcg gtggcgcaca ggacggcagg tgtcgccgcg tcgaaggtcg
    85921 cgaagaagcc gtccccggca tcgtcaacct cggatccacc ccatcgatcg aggagatcgc
    85981 ggactcgccc gtggtgggcc ctcaagagca gagaccatcg ccggtcgccg agcgtggccg
    86041 ccaggcgtgt cgagccgacg aggtcggtga agagcagcgt gaccaagacg cggcgttgag
    86101 ccccgtccca ggtcgctgag cgatatcgct cttcctggcc catggcggac catcccgtcc
    86161 tttcccgtcg gcgatgccgg tgaagcggcg attgctgtcg tgctgtgggg aagtgcgaag
    86221 ctgatcgagg acatacggtc tccgttcgcg tgcgaacgcg gctcgggagg tcttccctct
    86281 gatgaacgaa ggccgcccga agggcggccc cagacgtcgg aatcggaccg gcgcttaggg
    86341 gagagccccc gcgggacgcg tcgtgaaggc aagggtgtcg atgccgggtc gccgacccat
    86401 cgttccctcc ccttctcgtt cggtcgtcat cgaggacgga ccctagacct accttcgtct
    86461 gtaacgcaat ggcccatcgg gagggtttcg actgtctgac gcacgtcgct gacgcagcga
    86521 tcgagccatc gctacgcctc tgagtgccac actgctaccg cggaccatcc gctccttttc
    86581 cgcctttgac caaattggcc tgattctcgg tacgaacacg cccgctcgcg tcctggaacg
    86641 gacggtaggc gtccgtgatc ttgccccaca cgcgctcctg cactgaggtc gggtggccag
    86701 cgagcatcgt ggtcaactgc ggggcgacgt cgcggatgaa gtcggtgaac tcctccggcg
    86761 agtccgtctc gaagatcacg gggagcgtcc ccgtctcgac gtcgcggaac cccgcatcgg
    86821 tcacgagcgc agcgagcttc gcgagatcgc tgagcgcgaa cgcgcccggc ctccccggcg
    86881 gcggaggcgg cagctcgagc tcctgggaaa cgatggggaa cgccgccgcg aactgcaccg
    86941 ctgggatcgg tccccacacc gaggccgcga gcgtgcctcc gggccgaagg aacccatgca
    87001 gccgccgaag cgtgccgggg acgtccggca ggaacatcag cgtggcccgg ctgatcacgg
    87061 cgtcgaaggt ctcgtcgtcg tagtcgagat cctcggcgtc gcgagcgacg aactcgacgt
    87121 tgtggatgcc cgcctcggtc gctcggtccc gggccgtggc gagcatctcc tccgagatgt
    87181 cggtgcagac cacacgtcca tccggtccca ccacgcgagc agccgacagg ctcggctccc
    87241 cgtagccgcc gcccacatcc agcacgcgat caccggctcg cagttcggcg agctcgatga
    87301 gcttggcgct gtgccgctga ccgccctcgg gaccctcgac cacgtcgagc caccggcgcc
    87361 agccgccggc ggccgatcgc cactccgttc ggatgttccg cttgaactcg accggatcga
    87421 acgcgctcga cggagccatc ggcccccttc gtacttcgcg aggagatatt agggcgaccg
    87481 gaacgcgcct cgctcccgcg atacgctcga gcgccgatga gcccgaagat gaagaagtac
    87541 ggaacggtcg acgagtacct cgccgatcag ccggacgagg tccgggagac cctcgaacgc
    87601 gtgcgtcgct cggtgctcgc cgtcgtgccc aaggcgaccg agaagatcgg gtacgggatg
    87661 ccgggcttct acgtcgaggg gcgtccgctc gtgtactact cggcgttcaa ggaacactgc
    87721 agcctcttcc cggcgagcgc tgccgtgatc gagcggctct cggacgatct gaagggatac
    87781 ggtctcgcga aaggcacgat ccgtttcccg atcggcaagc cgccgccggc gccgttgatc
    87841 aggaagatcg tgaaggcgaa gctcgaggag ctcagaggct gaacgacccg atcgagttca
    87901 ccctgttgaa cggaccacgt gagcctccgg cgtctggctc gggtcgtcga ccgtgcaggc
    87961 cgccagcccg atcagtgtga gcccgacgag gatcggccgt gccattccca acgtcacgag
    88021 aatctgaacg agcccacgat ctggccgccg ctcctgaaga ggtcatcgtg tgacgcgcct
    88081 cccgggccat cctcgatatc gatgaccatg accccgtcac ccgaatccag cacgtaccac
    88141 cgcaacgaca tcccggggag catcgcccag ttgtagccgc cgacgtcagc cttgctgatg
    88201 atcaggggcg ccgtaggcat cgggtcgctg tagaagcacg tctgcttcca tccgggatcg
    88261 agtcgtagat cgagaagcat cccgtcgagc ccaccgaccg tcaccggcac cgcttcgctc
    88321 acgtcgaggc tcggcgtggc ctcgagccat gcgacgagct cccgcacaga cgaacccacc
    88381 cccggttcca cggattctgt ggccttctcg tcaccgcaat cctgggaggc gatcgcaggg
    88441 tcgcgaagga agtagatccc gtcggacgcg tagaggtcct gatgagactc atccgcgatc
    88501 atcgcgaagc tgccggagta cgaccatgtc cacccgggcg atctgatctc gaactcgatc
    88561 gtcggatcga agtactcggc ccggtagcgc ccgggatcca gtgccccctg acaatcgaag
    88621 agagggcaat ccgaaccggt gagcagttgc acgctcagtg acggcgtgga cgtctcttcg
    88681 gtcggatcgg cgggttcagg atctcgagcc gaacacgatg ccaccaggag catcgcgagc
    88741 accgaggcgc tcaggccaga gaccgtcgat cgttgtccgc tcatcaaccc gtcgcctctc
    88801 agctccgacc acgacgtcgt ccttcgacgc acgacgaacg ctagggacac gcgtgacggg
    88861 aggaaacgac aggaccccta cgtttgatgc tcggaaaggg agggagcctg gcgctgggtg
    88921 acagacgagc cgtgctggcg agcgagctcc gtacgggacc tgatgccgag cttccgatag
    88981 atccgacgca ggttcgactc cacggtcttc acgctcatga agagccgctc cgcgacctcc
    89041 ttgttcgtat aaccctgggc gacgacatcc gccacacgtc gctcggtctc gctgagctcg
    89101 gtcggcgagg cggagcgacc tccgatccgc gcgagggctt gatcggcgcg tgcttcccac
    89161 aggcgtgccc cgagagcctc gaatcgggat cgggcgccct cgagagactc tcgagcctct
    89221 cgcttcctct tcatgcgtcg aagcgtctcg ccatggatca gaagggtgcg cgcgtactcg
    89281 aaggggatcg caacacggtc gtgctccgcg aacgcccgct cgagctcgag cagtgcgccg
    89341 ggtgggtccc cgagagccgc ggcgatgagc gctctacatc tggccgccgt ggcgagcgcg
    89401 agcggccgat ccaggttcga gccgtgctcg tggagccgat cgacgacctg cttggctcgg
    89461 tcgaggtgcc ccaaggagat caacgcctcg gcgagatcgg ggatgaacgg gaacgcgccc
    89521 ggctcgacga tcccgttggt caccagcagt cgatccgccg agtcgagggc acggacgacg
    89581 ccgtcgagat cgccgaccga gagcgcgatg aacccgagga cgctgcggtt ctcgacctcc
    89641 gtccacaagt tgccctgggc ggcagcgagg gaggctcccg tcgatgcgtc tgtgcgagcc
    89701 tcgtcgacgc gtccctcgag ggccgcgacg tgcgccctcg cgtacagcat ctgatcccgg
    89761 atctcatcca ggcctgcatg agcgatgatg tcgtatccct cgtccgcgtg gcgagctgca
    89821 tcggcccatt cccctgccgc gcactccacc tcgctgagaa cggacagggt gtcctgacgc
    89881 atcagctcga ggccggtttc catgaggtga aggtcggact ccgcgatcgc gcgctgcgcc
    89941 tccgcgatcc ttccttccca gagcatctga tggccacgga tcctcgtcgg ctgggtagtc
    90001 tctcctggag cgaggtcgcc ggcgatcgcg accgcacgat cgatcgtcgc ccaaggatca
    90061 gcgccgagca gcgcttctgc ctgggcgagg gctccgagcg ccaggcggag cggggtcgga
    90121 tccgatgcgc gttccgcgag ctcggcggcc gctcgagcgt gatcgaccgc ccgtgacggc
    90181 tccaaacgac agagtgaaac ccacgcgagg ttcatgtggg cgaccgggag gaagatctcg
    90241 tggtcgccga cgtccggaag cgccgcgcgg acgagttcgt cgacccgatc ggcatcgttg
    90301 taggaccgag ccgccagctc cacccgcgtc cacgcctgtt gctctcgaga ggtggccgct
    90361 ccgatgaggt cctccaacat cgttcggcct cgctggacat ctccggcatc gaatcgttcc
    90421 agcgccgcgg atcgcttcct gctgaagagg agcgcttcgt catccggcgg ggtgagatcg
    90481 gcggagagct cccacagctc ggctgcggcg aggggcgctc cccgacggcg cgcgtggccg
    90541 gccgcctctt ggacgaacga cgctcgttca ggatccgggc ctccgcccat caacgccata
    90601 tgtcgcgccc gttcctctgg gtcgaggacg acctccgcga tgcgcgcatg gatcgcctgt
    90661 cgtcggccgg tcgaggccga ccagtaggcc gtggagcgca tcagcggatg ggcgaagtcg
    90721 agccgctctc cctgagcgac cacgagtccg gcgtcctcgg cctcgaggag acctgcatcg
    90781 accatcgcgg gctcgcggct cgtggcccgc agcgtggcaa gcgtcggacg cgccatcgct
    90841 gccatgagga gcatcacgtc tcgagcgctc gccgagaggc cgtcgatacg agcgcgtagg
    90901 atgtcgaggg catcgccggg aaccggaagc ggccggcccg ccgccggttc ggcgtcacgg
    90961 agcgcatgga cgatctcgag cgagaagaac ggattgccgt gcgccgcttc gtgtacgagc
    91021 gcgacgagcg gcggcgtcac ggatcggctc atccgctcgc ggaggatccg ggcaacgtcg
    91081 tccacggcga ggcccccgac gtgggtgcga accgcgccgc gatcactgag ctcgcgcgcc
    91141 aggtcgagtg tctctgaagg gccgtgctcg aggcgcgtgg tcgcgaggac cgcgaccgga
    91201 tgaccctcca gacgccggat cgcgaaggag agggctcgag ccgatgagcc atccatccac
    91261 tggaggtcgt cgatcgcgat gacgaccggc cccgaggcgc tcagcgttcg gaacgcaccg
    91321 agcaccccga gcgagatcgc gcgctggtcg ggtcggaggg cgccggtctc ccgatggagg
    91381 agagcgatct ccagggcttg gcgttgcagg ttcggagcct cggcgagcgc ctcgtcgaac
    91441 acatccccga tcaggtcccc gatccccgag taggacaagc ctgtctcgtt ctcactcggc
    91501 cgagaaacaa gcacgcggtg accggcctcc tgagcggctt ccactcctgc tcgccacagg
    91561 accgtcttgc cgatccccgg ttcaccttcg aggaggaggt cctgcggctt ctcgctcagg
    91621 tcgcggacga atcgggagag cgcggcgagt tcaggttcac gaccgacgag cccgcccggt
    91681 tccggtgcca ttgatctcct cgtccctgcc cagggccact catcgtagac gtcggcctct
    91741 ccacggccga gccgcgcgcg ggaggcgagt acgtgggtcg cttcccccct ctgagcacgc
    91801 caccattccc tgaggagagc gcgaacccgc acccgatgtt gtagctagcg cttccaggtc
    91861 ggcgatacca gagatcaggg gcgggccaga cacgggcgtg gccgctcgaa ccgatcccct
    91921 ccgctatcgt cgcccgcgtg gagaggctcc cggtcgaggt cgtgtcggtg ctcgtcggaa
    91981 ccacatgggg actggcggcg atcttctggg tcctgatccg ccgctgggag cgaacgggag
    92041 tcaccgaggc tcgccggaag gagctcgagg gcgagggacc ggaggccgtg tcacccgcga
    92101 tccgggcctg gcgatggatg accgtcacga tcacgggcgc gatcccggcg ctgttcgcgg
    92161 tcgacgggtt cgtcgacccg gtcgagatcc tctacgcgcc ggagctgtcc ttcttcgccg
    92221 ggcccgacct cgcgttgcag atcgtcggag tcgttctctc cgccgcggga ctggcgatcc
    92281 tgatcggcgt ggggcggaag ctcgcggtca acgtgtaccg ccgagcgatc gacgaacggg
    92341 agatgatgac gaccggtgcg caccgctacg tgcggcaccc cttctacatc cacttcctcg
    92401 tgctgccgat cgggctgtgc ctgctgacct tgaactacct ggcgctcctc gtcctggccg
    92461 cgtatacgac gctctggcag ccgacgacgg tcgtcgggtg gatgcgcctg gaggaggacg
    92521 agctgcggcg ccggtacggc gacgaggccg aggcgtatct gagtcggacc gggcgcgtct
    92581 tccctcggct cccacggccg tgatccggct ccgggagaac gcggccctcc ttctcggagg
    92641 gccgcgtccg ttcccctgct ttgcgcgtta gacggtcgga ccctcgagct tcctgcgggt
    92701 cgcccagagg gcgagcccgc cgatcagggt gagggcggcg gcgaggagcg accacagccc
    92761 gacctccgat ccggtgaagg gggtcccacc gagaccaccg ccgccaccag cgatgcagca
    92821 gccggggcca tctccgcccg agccggcgat cggcgtgacg accgcgtcgt catcgtccgt
    92881 caccgtccga cccaggatgt cggtccccga agcggtcccg atgttgatgg tcggtgagtt
    92941 cccaaccgag aacgtgccgg tgcgcgtcgc ggacgcgccg gcggcgaggc tcgcgatcgt
    93001 gccgatgtgc ccgatcacgt catcgtcgac actgatgtcg aacagggtgg tatcgccggt
    93061 gttggtgacg aggtacgtgt aggtcaccac ggtgcccacc gggacggtgg cgtccgacac
    93121 ggtcttcacg atcgtgatcc cgggatggat gatgtccacc tggaacgtgt cctggtcggt
    93181 gacttcgtcc ccacccccac cctccacggt gtcgccgcgc acggtggcgg tgttcgggat
    93241 cgggtcggga tcgtcctggg tgaccagatg cgtacacgag aagtgccaga cctcgtccac
    93301 cgccaggctc ccgtcgacgt cgacgccggg gtcgatcgtc ccggcgtcgc accgcgcgtc
    93361 cgtcaggtcc acgtcgaaga gctcgacgtc cccggtattc gtgacctcga actgataggt
    93421 gatcgtgtcg ccccggtgca ccagcgccgg cccgccgccc ttgatgatct ggatcgccgg
    93481 acccggtggg gtgacctggg tctggcagga ggcggtgtcc gagtcgtccg tgtcggagac
    93541 cgcgcccgtg ccggtcgccg tcacggtgtt ggtgacttgg accacaccgg gtgccggcgt
    93601 gtgagtgacg gtcgcggtcg cggtcgcacc gaccgcgagc ccaaggctga gggcggaaac
    93661 gaactcatcc gtgatgtcac ccagcagggt gtcgtcgacg acgacgtcga cgagcgcctc
    93721 ggagcccgtg ttctcgacgg tgatcgtgta ctcgatctcg gccccggcgt cgacggagtc
    93781 cgggcaggac ttctcgacgt cgatctcggg ggtcggaggc ggcgggacgt cctccatcag
    93841 acagaaggag atgtgcgaga ggtcgtagaa cttcccgttg ctcgggttga ccggggcgtg
    93901 gagaccggtg tcggcggcgt tcccgtccgg ccggtagtcg tagaagttgg cgttcgagcc
    93961 gcccttcacg atcacgccgg cggcgacgaa gtcacccgtg agcgtgaatc ggaaggtctg
    94021 cgccccctcg ttgaccgcga cctccagcgt gccgctcaga gcgccgaacg tgagggtgat
    94081 cgtagcgtcc tgtggtggat cctgcttgcg ctcgaacagg aactgggccg aggtcatgac
    94141 gtccaggcag tcgggattgc ccttcacgaa gatcggctgc tcgccgtcgc cagaaggggg
    94201 tcccggggtg tcgacggcag ccgcctcggg cgccgatccg gcggccgcct cgtccgaagt
    94261 gccatcgccg gtggcctccg caggttcctc cgtggcgacc gttcccgtgc cctcatctcc
    94321 ggccgaggga tcctccgcgc tgacttcctc tgccgccgct acctcttccg tcgcaacctc
    94381 ttcggtcgtc accccctccg gtggaagggc ctcctccgag gtagtagtgg cctccgccgt
    94441 cgcgccgtcg gactcgatcg tgggctgatc cgccacggcg atcagcgacg cctgtccgag
    94501 cgcgaacgcg aacaggatga gcagtgacgc gagcgtcccc cgtccccggc ggcgccggcg
    94561 gctggatggt gcggggccaa gctgccaagc gccgttggcg cgccaggatg tgctgtcctt
    94621 catccttccc tcccaagatg tcgtgctgtt gacctaccgc ggttacccgg gtggctgcgg
    94681 gtgcaaccgt acgccgatgg cgacgggctt gctgcggata atgtcctgga agtggaaggg
    94741 atcgccggat acttcacggc cctcgggctc tcgacggccg ccgggctcaa cgcctatatc
    94801 ccgatcctga gcgtcggcct gatctcccgc tccaccgatc tgttcgacct cccggcgccg
    94861 tgggacgcgc tcggcgaccc gatcgtgctg ggcatcgtgg gcgtggtcgg cgtggcggac
    94921 ttcatcgggg acaaggtccc gatcgtggat cacgtgttcc atgcggtcgg tctggcggtc
    94981 gcgccgatcg ccggggggat gctggcgctc gcgtcggcga gcgcgttcga cctcgatccg
    95041 gcggtggcgg ccggcatggg gatcgtcgcg gcgctcgcga cgcaggcggg gcgcaccgcg
    95101 gcacgcccac tctcgacggc cacgacgggc gggggcggga atcccgcggt gagcctggcc
    95161 gaggacggcg tctcgggggt tctgtcggtg acggcggtgt tctggcccgt cgtcacggcc
    95221 gtgctcgccg tgatcgtgct gggggcggtc ttcttggtct ggcggaagtg gcggcaactc
    95281 cgggcacggt tccggcgccg cgatgttccg gtgacgtgat cctcggttgg tcggaagccc
    95341 ccgataggct gcccgccgat gaacgccaag gagatcgccc gtcggcggat cggcaattcc
    95401 cgcctcacgg gcgatcggtt cgaggccccg gacgacgtcg tccggtggca cctcgcgatg
    95461 caggcccaag agtacggccc ggcgaagtgg tcgatcgggc agcgatcgaa gggcgtcgtc
    95521 gacgccgacg tcgacgccgc ggtcaccgag ggcacgatcg tccgcaccca cgtcctccgg
    95581 cccacgtggc acttcgtcgg gagggatgac atccgctggc tgctcgcgct cagcggtcct
    95641 cgcgtgcagc aggggaacgc gggcaggatc cggcagctcg gactcgatgc tcgcaccctc
    95701 acgcgtgccg aggaggtggt cacctcagcc ctcgagggtg ggaaccgcct cacccgcgac
    95761 gagctcgcga ccgagttcga tcggcggacg ctcgatcgcg gcggtcagcg tatgccctac
    95821 ctcctgatgc actgcgagct ggtcgggctg atcggcagcg gagggcggaa gggcaagcag
    95881 cacacgtacg cgctcctgga cgaacgggtg cccgcgcacc cgcgcccgct cgatcgggac
    95941 gacgccctcg tcgagctggt ccggcgctac ctcgacagcc acgggccggc gaccgtgaag
    96001 gacatgagct ggtggtcggg gttcacgatg accgacatcc ggcacgcgat cgacctgctc
    96061 ggcgacgagg tggcgagcga cgacgtcgac gggatctcgt tcgtgtcggc atcgtcggaa
    96121 cggagcgcgc gagcacctgt cgcgggcggt gcccacctcc tccagacgta cgacgagctc
    96181 gtggtcggct acacggagtc gcggttccac ggagaggcgg tcggcgatct cgcgcgcgag
    96241 gcgtggaacg accgcacgta cccgaccggt gtcttcctcc tgcgcggccg ggtgttcggc
    96301 cactggcggc gcacgctcga cgcgaaacgg atccgggtcg cgatccacac ctacgccgac
    96361 gccaccgcgg cggacgagcg cgcgctggga tcggctacca ggcgcctcgg ccgcttcgtc
    96421 gggctcccgg tcgaggtcga gatctcgcgg ttccgtcgct cctagcgacg ggtatcgttc
    96481 gctgatgcgc acggatctca cgatcgagga tctggacggt ttcctggagg agcccgtcgt
    96541 cgccgtgttg gcgaccatcc ggcaggacgg cagcgtgctg ctctcgccgg tctggcacga
    96601 atggcgggac gaaggcttcc aggtctggtc gggcccgaac gacgtgaaga cgcgacatct
    96661 gcgccgcgag cctcgcgcct cgatcgtggt ggccgagtcg actcacccgc tgcgaggcgt
    96721 cgaggttcgc ggcacggccg agatcctcac cgagaacggc ttcgagaccg ccgtcaggat
    96781 cgccacgagg tacctcggtc ccggcaaagg ggaggcctac gtccggaagg tcggcgggaa
    96841 cgacatcacg atccggctcg agcccggcga gctccgggtc tgggatttcg cggacgagca
    96901 ggaggggatg ggctcctaaa ggtatcgggc cgccgccatc ccacccacgg cgaccacgag
    96961 cagcggcacg atgacctgtc cgacggcctt cccgcgctgc tgaagcgcct ggagctgcgc
    97021 cccctgctcg ggggtgggcg gtccgccgct cgccgccacg tcccggccga tcgcgatggt
    97081 cgccctgatc gtcggccgga ccacgctgag accgagcgag aaggccgcga tcccgcacag
    97141 cgcaccgatc gtcagcgaga cgccttgcgc ggacgtgagc cagtcgccgt ccaggcctct
    97201 cgagacctcc cagtacatca gcaggccggc gaggatcgtc aggcctgccg cgcgcagcac
    97261 ccagtccacg agacgtttct tgccggcgag atgcgccatg aacggcccgg ccgccggacc
    97321 cacctcgcgc gcggtcggct ggaggaacag gatcgtcgtg aacgcggcgc ccacccagaa
    97381 cacccccgcg aggatgtgca ggatccgcag cgcgatcatg taggtatcca tcccgcgcgg
    97441 acgctagcgg gccccgatcc acggggcaag ccccgttcgt ggcggtcagc ctcggcgatc
    97501 gatcgagggg agcagctcgt ccttcgcgaa cgcgaggaac tcctcctgcc gagggccgac
    97561 ctgatgcagg aacacgtgat cgaatccggc gtcggtgaac atcccgatct tctcgacgat
    97621 cgcgcccgca tccggtccgc agaccaccct ctcggcgacc tgctccgtcg tgatcagctc
    97681 ggcgacctcc tcgaagtgct gcggctcggc cagctccaga ccgacctcgc ccgggacgct
    97741 cgtgttcggc caccactcga gcgcctcctc cttcgccttg gcctcgtccg gtccgtagca
    97801 cacggtcagc tgcccgtaga cgggccgacc ctcaccgccg gcgccccgga aggcctccac
    97861 gatctcggcc tcaggggcgg tcgagatcaa ggcgtccccg gctcgtcccg cgagtctggc
    97921 cgcggccggc gcggaagcgg cgacggcgat cggaggggag gtctcgggga tcgtgtacag
    97981 gcgagcccgg tcgatcgtgt aatgcggtgt tcgttcggtg accaggtccc cggtccacag
    98041 tcggcggatc agctcgatcg cctcctcgag catgtcgagc cgctctccgg actccggcca
    98101 ggccgtcccc gtgacgtgct cgttcaggtt ctcgccggtg cccaagccca gcgtgaatcg
    98161 gtccgggagc agggacgcgg tcgtggctgc ggcctgcgcg acgatcgccg ggtggatgcg
    98221 catcgtcggg cacgtcaccg cggtcatgac cgggatctcc tcggtcttca cggcgagccc
    98281 gccgagcaca ctccacacga aggggctttg cccctgccgc gaggtccacg ggtggaagtg
    98341 atccgagatc gtgaggaagt cgaagccgga ctcctcagcg atccgcccgt agaggaggag
    98401 gtcgccgggg ccgtgttcct cgccggagag ggtgtagccg atccgcacca tggtcggtag
    98461 atgtccgcga tggagccgat acaaacctgc tcacggatgg catgatgacg tcgccgggag
    98521 gccccgactc cccgttcgcg ccgaccgaag agggagggaa ccgatggcga ggctgttctc
    98581 gatccgtccg acctggtcgt tgaagggccg ccgcttccag gggctccgag ggttcaccgg
    98641 caagccgttc cacccgccgc tcacggactt cccgatcgtg tgctacacgc tcgccgcgat
    98701 cttcgacctg atctcgttct tctcggacga gatggatccg aaggcgcacg acttcttcgt
    98761 gtcggcgacg ttcgtgatgg tcgccgggtt catcgtgagt ctcggcgcgg cgctcacggg
    98821 cttcatggac tggtggaagg gcatcccgcg ggaccggaac agcggcccga tcggcaaagc
    98881 caagcacaca caggtctggc gcacgatcaa ctggcacgcg accgtgatgc tgacggtcac
    98941 cgcgatcacg ctgatcgaca tcatcctccg cgtctccgac ttcgactcgg ggcagacgca
    99001 gggttcgtgg ctcgtgctct cgctgatcgc ggccgctctc gtgacgttcg gcgccgcgta
    99061 cgggggcacg ctcgtgttcg agtacgggtt caacgtcgag aacgtcgagg aggtctggaa
    99121 ggagtccgag gaggaccacg tccggctccc ccgcggcggc tcctcttcgt aaccggcgtg
    99181 gccgcggatc gtccgctcgg atacgtgacg cagacgtcgg cgacatcggc cgcgccgccg
    99241 caggtcgtat acgagacgat cgccgacctc cggaaccacc tcgactggtc cggggatcgc
    99301 gccagcagcg agacgttcaa gctgctctcg atcgaggcgc ccgacggtcc tgcgagcgtc
    99361 gggaccgcgt tctcttcgtc cggcgcggcc gacaacggca cgttccacga ccgatccgag
    99421 gtgacggtcg cgatggctcc cagctcgttc gtgatcgaga ccgacgcgca tctggatcgg
    99481 aagcgcggga agccgtggga cgcgcacttc atccaccgct acgacgtcgc gccggaaggc
    99541 gacggctcgc ggatcacgta caccgagacg atcgagcgtg tgaactacgt gccctactgg
    99601 ctccagcccg ggatccgatc gatcttcaag gtctacgtga accgagcgga ccggaagcag
    99661 ctggggaacc tcgctcggct cgccgaggag cgctccgcct ccggccggta gctcagaact
    99721 cgagcgtgca gttctcgcag gcgaatcgct tcgcgcgatg gttgtggctg tgtccgccgc
    99781 accgatagca gcgccggcac gtgccccccg agtgacagtc gatcgggtcg gtcgtctccc
    99841 cgggttggtc ccacgtggtc gccggttcgg cagggcgggg gagctggatc gacagaagcg
    99901 tcatcggtcc gtccccgctg acgaagccgt gcgggtgcgc ggtcgcccgg ccgatcacga
    99961 ggctcccctc gagcggaacc gggttgtccg caagcagcgc cttgccggcg ccctcgagca
   100021 cgaccaccca ctcctcggag tccggatgcg tgtgcgcgta cgcgggctgg ttcgccgtga
   100081 acccttggag cgacaggtag aactcgtccc cctgcgtgat cgggacccgg cggaacccgg
   100141 agggacagag cgcgagaacc tcctcgggat gcatcacgaa gacgccgagg ttgtccagca
   100201 cctccgcgag gtcgccgacc gagtgaggca tgggtagcat cctcccgcat cgggccgggg
   100261 cagccaaacc gaggccgtta cgtcctcgac gaggcgggga ggatggccgg aaagaagaga
   100321 gaggagggtg tcatgaccga gcggttgatc atcgtggacc atgtcctgtt cgtggtcgaa
   100381 gacttcccgg cgagccggcg cctctacacg gcggcgctcg cgccgctcgg gatcgaggag
   100441 ctgaacgagg agccgaactg cgtctcgtac ggtcggcaag atctcgacga cttctcgatc
   100501 tgcgagggct ccccggtcac caccgcgtcg catgttgcgt tcgatgcagc ggaccggtcg
   100561 caggtggacg ccttcttcga ggccgcgctc gccgcgggcg cgaccacccg cggcgagccc
   100621 ggcgtctgga tccagtactc ggaccgctac tacggggcgt tcgtgaacga ccttcacggc
   100681 aacaacgtgg aagccgtgtt ccacagcccg gtgccgatca cggacgcccc ggaacgcggg
   100741 ggagctccgt agatcgagcg agaagcggag aacccctgcg gtgcaggggt ttcaatcgat
   100801 tttcgttatc tcatcccatc ctcgtttcgg tcccccacgt cccgggtagg ggtcaaggtg
   100861 aagtccccaa caggcacgaa tcgaccctgt gaaggaggtg cccaatgagg actgatctct
   100921 ggaactaccc ggaagggacg accacacccc tggacatcga ggggttcaag gtgcgggcca
   100981 cggacggtga gatcggcaag atcgacgagg ccacgtacga cgtggattcg gcgttcgtcg
   101041 tcgtcgacac cggtccgtgg atcctcggcc ggaaggtgct gatcccggcc ggcatgatcg
   101101 atcggatcga cctcgatcga cgtgatgtcg aggttcggct cgcgaaggac caggtcaaga
   101161 acgcacctga gctcgacgag gtgaccgact accggtcgga cacctaccgc gagcaggtcg
   101221 ggacctacta cagcacctac agctaggccc ggcgcgtcat cggaacgagg cgagggcatc
   101281 cgccctcgcc tcttccgtct gcgggagtct cgcaccggct ggcccgctag gtcagctcct
   101341 ccttcgacgc cgcgaacgcc tgcacggcga acgacaggtc ctcgcgcgtg tgcgccgccg
   101401 agacctgcgc gcggatccgc gctttgccct tcgggaccac ggggtaggag aacccgatga
   101461 cgtagacgcc cctgtcgagc atcgcctccg ccatccgccc cgcgagcgcg gcgtcgccga
   101521 gcatcaccgg cacgatcgga tgctcgccgg gcagcacgtc caggccggcg gcctccatct
   101581 ccgcccggaa gaacctggtg tgctcctcga gccgatcccg gagctccgtc gacgccgtga
   101641 gcagctcgat cgccttcagt gatccgccca ccaccgccgg cgcgatcgtg ttcgagaaga
   101701 ggtagggacg cgaccgttgg cggagcagct ccacgacctc ccgggggccg ctcaggtacc
   101761 cgccgctcgc gccgccgagc gccttgccca gcgtccccgt gaggatgtcg acccgatcca
   101821 tcaccccgtg atgctcgggc gtgccgcggc cccccggtcc catgaacccg accgcatggg
   101881 agtcgtcgac catcacgatc gcgtcgtgcc gctcggccag gtcgcagatc gccgggaggt
   101941 cggcgaggta gccgtccatc gagaacacgc cgtcggtcgc gatcagccgc cgccgcgccc
   102001 cggccgcctc cttgagctga gtctcgaggt cgtccatgtc gttgttgcgg tagcggaacc
   102061 gctgcgcctt cgagagccgg accccgtcga tgatgctcgc gtggttcagc tcgtcggaga
   102121 tcaccgcgtc gtcctcaccg agcagcacct cgaagagccc gccgttcgca tcgaagcacg
   102181 acccgtacag gatcgtgtcc tccatgccga ggaagtcgct cagcgcatcc tcgaggtcct
   102241 tgtggagctc ctgcgtcccg cagatgaagc gcaccgaggc gagcccgaag ccccactcct
   102301 ccatcgcggc gtgcgcggcc gcgatcacct cggggtggtc ggcgagtccg agatagttgt
   102361 tcgcgcacag gttgaggacc tcgccccggc ggccgcgcac acccacccga gcccgctgcg
   102421 gcgtcgtcag cacccgctcg ggcttctcca ggcccgcacc gcggatctcc acgagctcgg
   102481 ccgcgagcgc cggtcggacg gtctcggtcg tcacggcccg tccgtccagt gcaggagcac
   102541 cttgcccgtc tcccccgtgc ggatcgccgt gaacgcctcc tcgaaccgat ccgccgggaa
   102601 gcgatgcgtg atcaccggcg cgatgtcgag gccgctctgc agcatcaccg tcatcatgta
   102661 ccaggtctcg tacatctccc ggccgtagac gccgcggatc gtgagctggt cgaagatcac
   102721 cgtgttccag tcgatcgcga acggctcctc ggggagcccc agcatcgcga tgctggctcc
   102781 gtgcgacagg ttctcgacca tgtcgcggag cgcggccggg ttcccgctca tctcgaggcc
   102841 gacgtcgaag ccctccttca tgcccagagc ctcctgcgtc tcggcgagcg agccgtcccg
   102901 cacgtcgatc gccacggtca cgcccatctt tcgtgcgagc tccaggcgat acgggttcac
   102961 gtcggtgatc acgacgtatc gggcgcccgc gtgccggacg accgctgctg ccatcagccc
   103021 gatcgggccg gcgcccgtga tcagcacgtc ctcgccgagg accgggaact gcagcgcgct
   103081 gtggaccgcg ttgccgaacg gatcgaagat cgccgccacg tcgcggtcga tcgacgcgtc
   103141 gtggtgccag acgttcgtca ccggcagggc gatgagctcc gcgaaggctc cggggcggtt
   103201 caccccgatc ccgcgcgcgt cggcgcacag gtggcgtcgt ccggccatgc agttgcggca
   103261 ccggccgcac acgacgtggc cctcgccgct cacgaggtcg cccggtccgt agtccttcac
   103321 gttggatccg atctcgacga tctcgcccac gaactcgtgg ccgatcacga gcggg
//
LOCUS       DASUYD010000078         4396 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_259743, whole genome shotgun sequence.
ACCESSION   DASUYD010000078 DASUYD010000000
VERSION     DASUYD010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259743"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VF195_04840"
     CDS             <1..368
                     /locus_tag="VF195_04840"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6580179.1"
                     /translation="SEHSDQATAAASELAKSGGTVHVLHVHEAGLVLPVETNTEAQGV
                     VDQAIKQLEADGVEVSGQAVSARAGSVAPTIVDAARSFGCDLIVMGTRGLSDFSGLLV
                     GSIAHKVIHHADCPVLVVR"
     gene            610..3321
                     /locus_tag="VF195_04845"
     CDS             610..3321
                     /locus_tag="VF195_04845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580180.1"
                     /translation="MATEAFQPLESDVVLRDGSTVRIRPARPRDETRVEDYLIGLSPE
                     TRRLRFWSQAIDVRGLARKIVDVDPENHLTLLVLHGGDEGRMIGGAQYIRTHGARAEI
                     SLSVTDEFQGRGIGSILLGQLAEAAAAHDIATFSADVLPENHSMINVFRSSGFDLSIR
                     ATPGAIEVEFPIGLTDTAIQHFEDRETQAAISAMRTFLAPRSVAVIGASRDSSAIGGR
                     LFHNLLMTEFSGSVFPVNPKADVVHGVVAYPNVEAIPGDVDVAFVAVPAMHVEEIAQQ
                     CGRKGVRGLVVISAGFAEVGDEGVQRQARLLETCRSYGMRLIGPNCMGIVNTAEDVRL
                     NGTFANVYPPAGRVGFSSQSGALGLAVMQYAAELGLGLSSFVSVGNKADISGNDLVAY
                     WSVDERTDVILLYLESFGNPRRFARLARETGRSKPIIAVKSGRSAAGQRAAASHTGAL
                     LAASDVTVDALFRQAGVIRTDTLEEMFDVATILANQPPPKGDRVAIVTNAGGLGILCA
                     DACEAHGLHVAALSEQSTAALRSFLSAEASVGNPVDMIASATASDYRRTIEIVAGDPG
                     VDAMIVIYIPPEATRAEEIGEAIAEGIDALEGRIPVATAWMSTKGLPAQLQKTGTRIP
                     SFAFPEQAAIAMARAAAYGRWREKPVGSVQTFDDARPDEAYAILATALGRRDDGWLRP
                     DEVERLLDCYGLRIARSERATTPEEAAEAAARIGGRVAVKAFGPDIVHKTEIGGVALG
                     LSGVDEVTRAVREITDRMAAAHISVKGFLVQAMVEGGVEMLVGVAHDPLFGPVVAVGA
                     GGTIVELLRDVSVRITPISDLDAQEMVRSLGTFPLLDGFRGAPKADVVALEEVILGIG
                     ALAENHPSVAEMDCNPVKVLQSGAVIVDARVRVEEPPPTKPLAGLGG"
     gene            complement(3468..3737)
                     /locus_tag="VF195_04850"
     CDS             complement(3468..3737)
                     /locus_tag="VF195_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009679953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6580181.1"
                     /translation="MKFESEVVPEALDRLRRVHGQLGGIIRMIEQGRDCQDVVQQLAA
                     VGRAVDRVGLKLLTAQLRQCLTDDVAARDEGYEEGKIERLFLMLT"
     gene            3891..4292
                     /locus_tag="VF195_04855"
     CDS             3891..4292
                     /locus_tag="VF195_04855"
                     /inference="COORDINATES: protein motif:HMM:NF025066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6580182.1"
                     /translation="MDGSLSLVLFSGTDDKLNAAAVLAAGAAAMGRKVHVFIQYWALD
                     AFRADRILKDHGVAPEAGLEGAEALQRHGGQHWSEILRQAKDIGEVDIQACSLSMEMF
                     GLEVADLDPLVDGQEGVAGFFLHADGSVTFI"
BASE COUNT          706 a         1435 c         1539 g          716 t
ORIGIN      
        1 gctcggagca ctccgaccag gcgacggccg cggcctccga gctcgcgaag tccggaggca
       61 cggtccacgt gttgcacgtc cacgaggcgg gcttggtcct gcccgtggag acgaacacgg
      121 aagcccaggg tgtggtggac caggcgatca agcagctcga agccgacggc gtcgaggtgt
      181 ccggacaagc cgtgtcggct cgggccggtt cggtcgcccc caccatcgtc gacgcggctc
      241 gttcgttcgg ctgtgacctg atcgtgatgg gaaccagagg tctgtccgat ttctcgggcc
      301 tgctggtcgg gagcatcgct cacaaggtca tccaccacgc tgactgccct gtcctcgtgg
      361 tgcgctgaca cggttagcgc ggatcgcagg gcgctccgcg ctgtgcaggg cggccccgca
      421 tgtgtggcgg cggagattgc ggcgacgcga atcggcaccc tccttccatc ggggcggcga
      481 tcgtcctcag gccgcttcga ccgtaggccc gcgaagcacc cgtgacctca ccgaacgtca
      541 cttccagcca gggaccttcg gccaagttga gcaccccccc gcctgccacg cttcactacg
      601 atggatgcga tggcgaccga ggcgttccaa ccgctcgaat cggacgtcgt tctgcgggac
      661 ggatcgacgg ttcggatccg acccgctcga ccgcgtgacg agacccgcgt cgaggactac
      721 ctgatcgggc tctcacccga gacgcgccgc ctgcgcttct ggtcccaggc gatcgacgtc
      781 cgaggactcg cccgcaagat cgtcgacgtc gatccggaga accatctgac cttgctcgtc
      841 ctccatggag gggacgaggg gcggatgatc ggtggtgccc agtacatccg gacacacggc
      901 gcgcgcgcgg agatcagcct ctcggtgacc gacgagttcc aaggtcgagg gatcgggtcg
      961 atcctgctcg gccagctggc cgaggccgcc gcggcccatg acatcgccac cttctcggca
     1021 gacgtcctgc cggagaacca ctcgatgatc aacgtcttcc gttcgagcgg cttcgatctg
     1081 tcgatccggg cgactccagg cgcgatcgaa gtggagtttc cgatcggcct gacggacacg
     1141 gcgatccagc acttcgagga tcgtgagacg caggcggcga tcagcgcgat gcggacgttc
     1201 ctggcgccac gttcggtcgc tgtgatcgga gcctcgcggg actcctcggc gatcggaggc
     1261 cggttgttcc acaacctcct gatgaccgag ttctccggct cggtcttccc cgtgaatccg
     1321 aaggcggacg tcgtccacgg tgtcgtcgcg tacccgaacg tcgaggcgat ccccggcgat
     1381 gtcgacgtcg cgttcgtggc ggttcccgcg atgcacgttg aggagatcgc gcagcagtgc
     1441 ggtcggaagg gcgtgcgggg gctggtcgtt atctcggccg gattcgcgga ggtcggcgac
     1501 gagggcgttc agaggcaagc gaggctcttg gagacctgcc gctcgtacgg catgcgcctg
     1561 atcggtccga actgcatggg gatcgtgaac acggccgagg acgtgcggct aaacgggacc
     1621 ttcgccaacg tgtacccgcc agccgggcgg gtggggttct cgtcgcagtc gggggcgctc
     1681 gggctggcgg tcatgcagta cgcagccgag ctcgggctgg gcctgtcctc gttcgtctcc
     1741 gtgggcaaca aggctgacat ctccggaaac gacctcgtcg cgtactggtc ggtcgacgag
     1801 cgtacggacg tgatcttgtt gtacctcgaa tccttcggga acccgaggag gttcgcgcgg
     1861 ctcgcccgcg agaccggaag gagcaagccg atcatcgccg tgaagtccgg gcgcagcgcc
     1921 gccggccaac gagccgctgc ctcccacacc ggcgcgctcc ttgcagcctc cgatgtcacg
     1981 gtcgacgcac tgttccggca ggcgggcgtg atccggacgg acacgctgga ggagatgttc
     2041 gacgtcgcga cgatcctggc gaatcaacca ccgccgaagg gcgaccgcgt ggcgatcgtc
     2101 accaatgcgg gaggcctcgg gatactgtgc gccgacgcat gcgaggccca cgggctgcac
     2161 gtcgcggcgc tctcggagca gagcacggcc gccctgcgat cgttcctttc cgcggaggcc
     2221 agcgtgggga accccgtgga tatgatcgcc tccgcgacgg cctcggacta tcgacgcacg
     2281 atcgaaatcg tggcgggcga ccccggagtc gacgcgatga tcgtgatcta catcccaccc
     2341 gaggcgacgc gggccgaaga gatcggcgag gcgatcgccg aggggatcga cgcgctcgag
     2401 gggcggatcc ccgtggccac cgcctggatg tccacgaagg ggctcccggc gcagcttcag
     2461 aagaccggga cccgcatccc gtcgttcgcg ttcccggagc aggcggcgat cgcgatggcg
     2521 cgcgcagcgg cgtacggccg atggcgcgag aagccggtgg gatccgtcca gacgttcgac
     2581 gatgccagac cggacgaggc gtatgcgatc ctcgcgaccg cgctcgggcg gcgagacgac
     2641 ggatggctga ggccggacga agtggaacgc ctcctcgact gctacgggct gaggatcgcg
     2701 cgttcggaac gggcgacgac gcctgaggaa gcggcggagg cagcggcccg gatcggcggc
     2761 agggtcgccg tcaaggcgtt cggcccggac atcgtgcaca agaccgagat cggaggtgtc
     2821 gcgctcggac tgtccggggt cgatgaggtt actcgcgcgg ttcgggagat cacggatcgg
     2881 atggcagccg cccacatctc cgtcaaggga ttcctcgtcc aggcgatggt ggaaggtggc
     2941 gtcgagatgc tggtgggcgt ggcccacgac ccgttgttcg ggccggtggt tgccgtgggc
     3001 gcgggcggaa ccatcgtgga gctgctcagg gacgtctccg tccgtatcac gccgatcagc
     3061 gatctggacg cgcaggagat ggttcgatcg ctcggcacgt tcccgctcct ggatggcttt
     3121 cgaggcgcgc ccaaggccga cgtagtcgcg ctggaggagg tcatcctagg gatcggcgcg
     3181 ctcgccgaga atcatccgtc ggtcgccgag atggactgca atcccgtaaa ggttctgcaa
     3241 tcaggcgcgg tgatcgttga cgcccgagtg agggtcgagg aaccgccgcc cacgaagccg
     3301 ctcgccgggc tcggtggctg agttaagccc ttgcctcgtc agacgatctg gccgatgcgc
     3361 gtccggctga cgaggagcca gccgagaccg aacgagccag cgatcccgcc gatagcgacg
     3421 acacgcctcg gtcgccgtga gcgccggtta cacgatcgcc gcccggttca ggtgagcatc
     3481 aagaagaggc gctcgatctt gccttcctcg tagccttcgt ctcgagccgc aacgtcgtcg
     3541 gtcaggcact gccggagctg ggcggtgagg agcttgagcc ccacccggtc caccgcccgg
     3601 ccgacggcag cgagctgctg gacgacgtct tggcaatcac ggccctgctc gatcatccgg
     3661 atgatcccgc cgagctgccc atggacccgg cgcagccggt ccagcgcctc cggaacgacc
     3721 tcgctctcga acttcatcgc ttgcacccct gtttcgcctc atcaggatgc atcctcgatg
     3781 ctgtaccccc cagggcatag gggccttcgg cctcaaccgg ccgggtctcc cggcactcag
     3841 tacgcggcat ccgagctggc acgctggcgt gcacgaggaa ggaggacggc atggatggct
     3901 cgctctcact ggtcctcttc tcggggaccg atgacaagct caacgcggca gccgtgctgg
     3961 cggccggtgc cgcggcgatg ggccggaagg tccacgtctt catccagtac tgggcgctcg
     4021 atgcgttccg ggccgatcgg atccttaagg accacggcgt cgcgccggag gccggtctgg
     4081 agggcgcgga ggctctccag cgtcacggcg gccagcactg gagcgagatc cttcgccagg
     4141 ccaaggacat cggcgaggtc gacatccagg cctgctcgct gtcgatggag atgttcgggc
     4201 tggaggtcgc cgacctcgat ccgctggtcg acggccagga gggggtcgcc gggttcttcc
     4261 tgcacgccga cggctccgtg acgttcatct aggaggtgtg gggatccatg acgacggtca
     4321 acatcagccg ttcgatcgac gcacgaggca tgccgtgtcc agggccgctg atgagcctga
     4381 tcggcgcgat ccgcga
//
LOCUS       DASUYD010000079         5373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_261163, whole genome shotgun sequence.
ACCESSION   DASUYD010000079 DASUYD010000000
VERSION     DASUYD010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261163"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1729
                     /locus_tag="VF195_04860"
     CDS             <1..1729
                     /locus_tag="VF195_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580183.1"
                     /translation="GNMLKPMLARGELRLVGATTLDEYRSHVEKDAALERRFQPVLVE
                     QPTVDAAIAILRGLKERYEVHHGVRIQDPALVAAAVLSDRYVTGRFLPDKAIDLIDEA
                     ASRLRIEIDSVPVEIDEVDRRIRQLEIERAALQKETDPASKERLERLEKELADLEERS
                     HGMRAHWEQEKDQIDRIRALKSELEETRAASERAERDGDLEKAAELRFGRIVELERRL
                     EEENARLQEIQSEQKMLNEEVTEEDVAAVVSKWTGVPVSRLMEGEMQKLIHLEAALHE
                     RVVGQDAAVTAVANAIRRARAGLQDPNRPIGSFLFLGPTGVGKTELARALADFLFDDE
                     RAMVRIDMSEYQERHTVSRLVGAPPGYVGYEEGGQLTETIRRRPYSVVLLDEIEKAHS
                     DVFNVLLQLLDDGRLTDGQGRTVDFRNTIVIMTSNLGAAVFQDPTASPEEREEKVLSD
                     VRAHFRPEFVNRIDEVVVFDALSREDIVKIVEIQLRGLQRRLADRKLTLELTDEATTY
                     LANAGYDPNFGARPLKRLIQREIQDPLALLLLSGDILDADTVVVDAGPDGLTPRVEPR
                     EDPGESAAG"
     gene            1794..2564
                     /locus_tag="VF195_04865"
     CDS             1794..2564
                     /locus_tag="VF195_04865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580184.1"
                     /translation="MKVRTSRILLLWLAVVLLGGMLALALAGDAEARRELPAQVAAWG
                     TIGLIVFGGWWFKVRPRRELHQEQARWLRLRSEPGDPLGFFERPFALLGHAAGAKDIE
                     NTSWGTWRGMEVVVIDYWYARSSDPSRNDHRYFTCAAASVPEGWPNLSIVPEGFASRL
                     AASLGSRGIEFELESFNRAFDVRCDDPRFAHALIDGRMMTWLEALPPDTGFEISDGTL
                     LCRTPRWPDGDVEWALETMRTFLDRIPPAFASLFGPER"
     gene            2646..3332
                     /locus_tag="VF195_04870"
     CDS             2646..3332
                     /locus_tag="VF195_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580185.1"
                     /translation="MDGLVPVLLLLVFGLLVAAVAYHQHQRKLARQREFHALALGHGL
                     DFSIDDPFDTLEEPFALLQRGDGRGVENVLWGEWKGLEIRAFDYWYYEESTDSKGHTS
                     RSYSRYDCVITRIDASCPALQISEENVFTRLADALTFRDIEFESEEFNRRFTVRGADE
                     RFATALCDAPMMDWLLRHGDGYAFEVVGDRVLCSCRRVPPAGMIHLFGTVKTFQEQIP
                     GVVSSLYPKG"
     gene            3371..4072
                     /locus_tag="VF195_04875"
     CDS             3371..4072
                     /locus_tag="VF195_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX6580186.1"
                     /translation="MEVLWVVLAIVVVLAIAGVVSYNRFVSQKNLIKDAWANIDTELR
                     RRYDLIPNIVETVKGYAAHEREVFENVTRARANATGATGSPAEQAAAEGPLVAALRQL
                     FAVVENYPDLKANQNFLALQAELTNTEDRLQTARRFYNANVRDYNRRVQSVPSNVVAN
                     LFHFEEEEFFEVEEALRGGEGVPRVSFEAGAPGVSFGSPSEPSTAPPATPPAGMPPPA
                     EPPPVAPGRPDEPAR"
     gene            complement(4076..>5373)
                     /locus_tag="VF195_04880"
     CDS             complement(4076..>5373)
                     /locus_tag="VF195_04880"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580187.1"
                     /translation="PSDTGLRASVSLLRRNRDFRALFFASVISLGGDWFLWVAINGLI
                     YEATGKALYVGLAILAQESAFFLASPIGGALADRIDRRKLMIACDLVRAVVCVSFLLV
                     GPETIWLAYVLLPVLATFAAPFDPAFSAATPNVVDPDDLPAANALNGSLWGTMLAVGA
                     GLGGLVSAAFGDDTAFIVDAVSFLVSAALLSSIRRGFSESRAETAEHPSIVEATRETW
                     RFARRDHRVLSLLAVKFGFGAAAGLLALIPVMALDADVFDSGNVGFGILMAARGVGAL
                     IGPFLGHRIAGPGHRRLFPAIGLSLAVFGLGYVAMGQAPSLAIAAVMICVAHLGGGSQ
                     WMLSTYGLQVLVPDHIRGRIFGFDYMLITLSLSVSALIASAVADQIGAPDTVTILGGV
                     ALVWALVWFVLSRGVRRTGLGGSTEPDPDTGSERIPSIS"
BASE COUNT          919 a         1753 c         1930 g          771 t
ORIGIN      
        1 ggggaacatg ctgaagccga tgctcgctcg cggggagctc cggctcgtcg gcgcgaccac
       61 gctcgacgag taccgatcgc acgtcgagaa ggacgccgcc ctcgagcggc ggttccagcc
      121 ggtgctggtc gaacagccga cggtggacgc cgcgatcgcg atcctccgtg gtctcaagga
      181 gcgctacgag gtccaccacg gggtgcggat ccaggacccc gcgctcgtgg cggcggccgt
      241 cctctccgac cgctacgtga cgggtcgatt cctccccgac aaggcgatcg atctcatcga
      301 cgaggcggca tcacgcctcc gcatcgagat cgactccgtg ccggtcgaga tcgacgaggt
      361 ggatcggcgg atccggcagc tggagatcga gcgcgcggcg ctccagaagg agacggaccc
      421 ggcatcgaag gaacgcctgg agcgcctcga gaaggagctg gccgacctcg aggagcgatc
      481 gcatgggatg cgggcgcact gggagcagga gaaggaccag atcgaccgca tccgagctct
      541 caagtcggag ctggaggaga cgcgcgcggc gtcggagcgg gcggagcgcg acggcgatct
      601 ggagaaggcc gccgagctcc ggttcggccg catcgtggag ctggagcgcc ggctggaaga
      661 ggagaacgca cgcctccagg agatccagtc ggagcagaag atgttgaacg aggaggtcac
      721 ggaggaggac gtggccgctg tcgtctcgaa gtggaccggc gtccccgtgt cgcgcctgat
      781 ggaaggcgag atgcagaagc tgatccacct cgaagcggcg ctgcacgaac gggtggtggg
      841 acaggacgcc gcggtcactg cggtcgcgaa cgcgatccgc cgcgcccgag ccgggctgca
      901 ggacccgaac cggccgatcg gctcgttcct gttcctcggt ccgaccggcg tgggcaagac
      961 ggagctcgcg cgcgccctcg cggacttcct cttcgatgac gagcgcgcga tggtccggat
     1021 cgacatgagc gagtaccagg agcggcacac cgtgtcgcgg ctcgtgggcg cgccgcccgg
     1081 ctacgtcggc tacgaggagg gcggacagct cacggagacg atccggagac ggccgtactc
     1141 ggtcgtgctc ctcgacgaga tcgagaaggc acactccgac gtgttcaacg tgctgctgca
     1201 gctcctcgat gacggcaggc tgacagacgg acagggccgg accgtcgact tccggaacac
     1261 gatcgtcatc atgacctcga acctcggcgc ggcggtcttc caggacccga cggcgtcgcc
     1321 ggaggagcgc gaggagaagg tcctgtccga cgtccgcgcc catttccgcc cggagttcgt
     1381 gaaccggatc gacgaggttg tcgtcttcga cgccctgagc cgcgaggaca tcgtgaagat
     1441 cgtggagatc cagctccgcg gtctccaacg gcggctcgcg gaccggaagc tcacgctcga
     1501 gctcaccgac gaggcgacga cctacctggc gaacgccggc tacgacccga acttcggcgc
     1561 gcgcccgctg aagcggctga tccagcggga gatccaggac cccttggcgc tcctgctgct
     1621 ctcgggggac atcctcgacg ccgacaccgt ggtggtcgac gctgggcccg acggactcac
     1681 gccccgagtc gagccgcgcg aggaccccgg ggagtccgcc gccggctgag cggccgcgac
     1741 ctcggacggg tcgatactgc tgtcatggag ccgcgggtgg ggggtggagg cggatgaagg
     1801 tccggacgag ccgcatcctg ctgctctggc tggccgtggt cttgctcgga gggatgctcg
     1861 ccctcgccct cgccggtgac gcggaggcga ggcgagagct cccggcccag gtcgccgcgt
     1921 ggggcacgat cgggctcatc gtcttcgggg ggtggtggtt caaggtgcgg ccgcgccgcg
     1981 agctccatca ggaacaggca cgctggctcc gactccgctc ggaaccgggt gacccgctcg
     2041 ggttcttcga gcggccgttc gcgctgctgg ggcacgcggc cggtgcaaag gacatcgaga
     2101 acacgtcctg gggaacgtgg cgtgggatgg aggtggtcgt gatcgactat tggtacgcga
     2161 ggtcgagcga tccgagccgg aacgatcacc gatacttcac gtgcgccgcc gcgtccgtgc
     2221 cggaggggtg gccgaacctg tcgatcgttc cggaagggtt cgcctcccgc ctcgccgcgt
     2281 cgttggggag tcgtgggatc gagttcgagc tcgagagctt caaccgggcg ttcgacgtgc
     2341 gttgcgacga ccctcggttc gcgcacgcct tgatcgacgg ccggatgatg acctggctcg
     2401 aggcgctgcc gccggacacc ggcttcgaga tctcggacgg gacgctcctg tgccggaccc
     2461 cgcggtggcc ggacggcgac gtggagtggg cgctcgagac gatgcggacg ttcctcgacc
     2521 ggatccctcc cgccttcgca tccttgttcg gcccggaacg atgacacgag gaggcatgaa
     2581 ggctctgcac gaccgatcta gggcgtgacc ttggctccgg acgggaccag gtcgatactt
     2641 cgaggatgga cggactggtc ccggtgctcc tcctcctcgt gttcggcttg ctggtggccg
     2701 cggtcgccta tcaccagcac caacgcaagc tcgcgcggca gcgggagttc catgcgctgg
     2761 cactgggaca cggcctggac ttctcgatcg acgatccgtt cgacaccctc gaggagccgt
     2821 tcgcgctctt gcagcgaggc gacggccgag gcgtcgagaa cgtcttgtgg ggagagtgga
     2881 agggtctcga gatcagggcg ttcgactact ggtactacga ggagtcgacc gactcgaagg
     2941 ggcatacgtc tcggtcgtat tcgcgatacg actgtgtgat cacgcggatc gacgcgagct
     3001 gtccggccct gcagatcagt gaggagaacg tgttcacccg gctggcggac gcgctcacgt
     3061 tccgcgacat cgagttcgaa tccgaggagt tcaatcgccg gttcacggtg aggggtgccg
     3121 acgagcggtt cgcgaccgcg ttgtgcgacg ctccgatgat ggactggctc ctgcgccacg
     3181 gcgacgggta cgcgttcgag gtcgtgggtg atcgggtcct gtgctcgtgc aggagggtgc
     3241 cgccggcggg gatgatccat ctgttcggca cggtgaagac cttccaagag cagatcccgg
     3301 gcgtcgtgag ctctctgtac ccgaaagggt aggcttcgga ttcggccgaa gcgagcgaaa
     3361 gggagcgaac gtggaagtcc tctgggtggt gctggcgatc gtcgtcgtgc tcgcgatcgc
     3421 gggcgtggtc tcgtacaacc ggttcgtctc gcagaagaac ctgatcaagg acgcgtgggc
     3481 gaacatcgac acggagctcc ggcgccgcta cgacctgatc cccaacatcg tcgagacggt
     3541 gaaagggtac gccgcccacg agcgggaggt gttcgagaac gtcacgcgcg ctcgggcgaa
     3601 cgcgaccggg gccaccggtt cgcccgccga gcaggcggcg gcggagggac cgctcgtggc
     3661 cgccctgcgt cagctgttcg ccgtcgtcga gaactacccg gacctgaagg cgaaccagaa
     3721 cttcttggcg ctgcaggccg agctcacgaa caccgaggac cggctccaga cggcccggcg
     3781 cttctacaac gccaacgtcc gtgactacaa ccgacgtgtc cagtccgtgc cctcgaacgt
     3841 cgtcgcgaac ctgttccact tcgaggagga agaattcttc gaggtcgagg aggcgctccg
     3901 cgggggcgag ggcgtcccga gggtgagctt cgaagcgggc gcccccggcg tgtcgttcgg
     3961 gagcccgtcg gagccctcca ccgccccacc ggccacgcct cccgccggta tgccgccacc
     4021 ggcggagccg ccccccgtcg cccccggacg tccggacgag cccgcccggt gagcttcagc
     4081 tgatcgaggg gatccgttcg gacccggtgt cggggtccgg ctcggtggag cctccgagcc
     4141 ctgtcctacg gacgcctcgg ctgagcacga accacacgag ggcccacacc agcgcgacgc
     4201 cgccgaggat cgtgacggtg tccggcgcgc cgatctgatc cgcgaccgcc gacgcgatga
     4261 gcgctgagac cgagagcgac agcgtgatca gcatgtaatc gaagccgaag atccgtcctc
     4321 tgatgtgatc cgggaccagc acttggaggc cgtaggtcga gagcatccac tgcgaccccc
     4381 cgccgaggtg cgcgacgcag atcatcacgg cggcgatcgc gagggaggga gcctgcccca
     4441 tcgccacgta cccgaggccg aacaccgcga gcgagagccc gatcgcgggg aagagccgcc
     4501 gatgcccggg acccgcgatc cggtgcccga ggaacggccc gatcagggcc cccaccccgc
     4561 gcgccgccat caggatcccg aatccgacgt tgccggagtc gaagacgtcg gcgtcgagcg
     4621 ccatgacggg gatcaacgcg aggagtccgg cggcggcgcc gaacccgaac ttcacggcca
     4681 gcagcgacag gacccgatgg tcccggcggg cgaagcgcca cgtctctcgg gtcgcctcga
     4741 cgatgctcgg atgctccgcc gtctcggcgc gtgattcgga gaacccgcgt cggatcgacg
     4801 acagcagcgc agcggagacc aggaaggaga cggcatcgac gatgaaggcc gtatcgtcgc
     4861 cgaacgccgc gctgacgagg cccccgagtc ccgcgccgac ggcgagcatc gtcccccaca
     4921 gggacccgtt caacgcgttc gcggcgggga ggtcgtcggg atcgacgacg ttgggtgtcg
     4981 cggcggagaa tgcgggatcg aacggggcgg cgaacgtcgc caacacgggc aggagcacgt
     5041 acgcgagcca gatcgtctcc ggtccgacga ggaggaagga cacacagacc accgcgcgga
     5101 cgaggtcgca cgcgatcatc agcttccggc ggtcgatccg gtccgccagc gcccctccga
     5161 tcggcgaggc gaggaagaac gcggactcct gcgcgaggat cgccaggccc acgtagagcg
     5221 ccttcccggt ggcctcgtag atcaggccgt tgatcgccac ccacaggaac cagtcgccgc
     5281 ccagggagat cacggaggcg aagaacagcg cgcggaaatc tcgattgcgg cggagcagcg
     5341 acacgctcgc gcgtagaccg gtgtccgaag ggc
//
LOCUS       DASUYD010000080        12638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_702607, whole genome shotgun sequence.
ACCESSION   DASUYD010000080 DASUYD010000000
VERSION     DASUYD010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12638
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702607"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF195_04885"
     CDS             complement(<1..704)
                     /locus_tag="VF195_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6580188.1"
                     /translation="MDHVTRRNALADRCADLEIDALLVTKLANVRYLTGFTGSSGMAL
                     VGPAGSVFITDGRYDEQSRHEVPDLDRVASMEDPAVAILEHVRGLGVQQLGFERHAVS
                     VAQFERWQERFDGLRLTGVGEEVERLRWSKDPEELTELRAAQEATDGAFEDILEVLVV
                     GITERQAAAQLELAMARHGADAPSFDVIVAFGEHAAEPHHRPCHRALGEGDIIKMDFG
                     ALVDGYHTDMTRTVAFG"
     gene            complement(692..1123)
                     /locus_tag="VF195_04890"
     CDS             complement(692..1123)
                     /locus_tag="VF195_04890"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEX6580189.1"
                     /translation="MKVLYLFGPNLGALGRRDPSLYGSESLEEIMVSVEERATGMGHE
                     VLWRQSDHEGDLVGWLLGAAAEDVGVIVINPGALTHYSYALRDAIESAGLPAIEVHMS
                     NTQAREDFRHRSVVSEVCRASIIGLGAGGYHLALEAMPWIT"
     gene            complement(1120..2196)
                     /gene="aroB"
                     /locus_tag="VF195_04895"
     CDS             complement(1120..2196)
                     /gene="aroB"
                     /locus_tag="VF195_04895"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEX6580190.1"
                     /translation="MTVPVPGRSYDVTIGSGLLPTLADHVPSLPGATTAFVVSDRTVA
                     DLHHPAIVGAIESRGLGTVLLLVPEGEEAKSLQAYGSLLRQLAGREAHRDDVIFALGG
                     GAVGDLAGFVASTYMRGVPFIQVPTTLTSQVDAAIGGKTAVNLPEGKNLVGTFAQPIA
                     VVADVDVLATVEERDFRSGLAEVAKYALTLDEGLLATLERDPGPIVRRSATVLEDVIA
                     RCVAAKAGIVAEDERDASARLVLNYGHTLGHALERLDAFEGRTHGEAVALGMVFAAAL
                     AEARGLAFGLRARTERLLGSLGLAPDGALPPVDEVLRRLRLDKKYRDGVRWVLLEDVG
                     RPIVVDDVSDTEVAAVLLEMGAPA"
     gene            complement(2193..2690)
                     /locus_tag="VF195_04900"
     CDS             complement(2193..2690)
                     /locus_tag="VF195_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX6580191.1"
                     /translation="MIVYLVGMPGAGKSTVGRELAGQLGVPFVDLDGEIEREAGAAVT
                     DIFRDEGEAAFRALEAAALVKASTQDPSVVACGGGVVLEPANRITLRNTGVAVYLDVP
                     LDELRRRVRPASDRPLIRQEGDLERLLASRGPLYREFAAHVVDGTGDPGVVAAAIVEE
                     LRWSV"
     gene            complement(2687..3841)
                     /gene="aroC"
                     /locus_tag="VF195_04905"
     CDS             complement(2687..3841)
                     /gene="aroC"
                     /locus_tag="VF195_04905"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017885082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX6580192.1"
                     /translation="MLRMLTAGESHGKALVAVLEGMPAAVPVAPKQIAAELARRRHGH
                     GRGGRQKLETDALDILSGIRHGTTLGSPIAITIPNAEWERKYRDLMSLEGEGDPADRL
                     TRPRPGHADLAGAMKYGFDDVRNVLERASARETAARVAIGAVCKALLTELDIRIVSHV
                     VRIGKVAVRAASRWPAPEDLEAVDASPVRCFDEAVAEKMVAEIDRVRKDRDTVGGEFE
                     VIAYGCPPGLGSHVHYDRKLDGRLALGLMSIQSVKGVGVGDGFASAARYGSAAHDEIV
                     RRDGRIARATARAGGIEGGMSTGQPIRLRAAMKPFSSVPKPLATIDLATREDAMAIKQ
                     RTDVCAVPAGGVVGEAVVAFELANAVVEKFGGDSLPEIRRNLDGFVAALP"
     gene            complement(3947..4513)
                     /locus_tag="VF195_04910"
     CDS             complement(3947..4513)
                     /locus_tag="VF195_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580193.1"
                     /translation="MALSERDRRTVTIGGSVVGVLLAGFVLFSVLGGGEEPFPPIPDT
                     PLPPSSPSPPPDGGPPIQSFTGRDPFSVPPALSPSPLPTAPGPSGGPSGSPSGSPSVG
                     PSGTPSPTAPGNDSSQNVGGSTVVLLDIFERDGATRVQVEVDGTVFDVGIGDQFAGGR
                     FELRSVAGNCATFLFGDESFTLCITAQK"
     gene            complement(4517..5104)
                     /gene="pilO"
                     /locus_tag="VF195_04915"
     CDS             complement(4517..5104)
                     /gene="pilO"
                     /locus_tag="VF195_04915"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX6580194.1"
                     /translation="MQGRRAIIVVGAGAAILALILIVFLVLPKMGQVTEAEKELQDTA
                     AEQQVLQARLNALEQARDEAPLNEATIRRIEQQVPPTADLSAVILFLRNAASVAGVQV
                     VSLTPAPPVASTTGGFSTISVSASGECSYFAMVKYLHEIETLPRAATVEAINLTPTEG
                     STLSFTATITLYTSDLSAGPGSEPGPTETVAVAGG"
     gene            complement(5094..5714)
                     /locus_tag="VF195_04920"
     CDS             complement(5094..5714)
                     /locus_tag="VF195_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580195.1"
                     /translation="MSQVNLLPPDILAAQRQRRLAGVVALAGAGVIGLIFMFYVLQLG
                     SLGSVREDIERAEQNNAGLQRDIEALQQFEEIRSRAQAKQDLLNQVYANEISFSGILM
                     DVSRVIPSDAALTSFSATAQEPTVTTGGSTLLTGRIDVAGLAIDYDTIASWLMRLERI
                     RGWVNPWVTSIADPETGPITYASGVDLTTAVVTERGQQAGGTADAG"
     gene            complement(5711..6766)
                     /gene="pilM"
                     /locus_tag="VF195_04925"
     CDS             complement(5711..6766)
                     /gene="pilM"
                     /locus_tag="VF195_04925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX6580196.1"
                     /translation="MPKTRIGLDVGSTAVRAAELAEGSPPTVVRAAQVPLPAGAVENG
                     EVREVEAVSESLRELWTRGGFKSRKVWMGVGNQRVVVREIALPTMPEKELRQSLGFQV
                     QEFIPMPVDEAVLDYHLIEEIEVDGRQMLRLLLVAAQKAMVDTLVTSATKAKLEPMGL
                     DLVPFAMVRAVGATGAGMELERSGGEAVVDVGAHVTNIVVHEAGETRFVRILPSGGRD
                     ITVAIARGLSVEDEVAERLKRGELVEGSDATPEQALQVAMQRATQFVDEIRSSLEFYT
                     AQTQGARIERLLISGGGSKLEGFIDIVRQRIPVTVEPGRVFSKVGSQLSLSEDAQAEA
                     EPVLATAVGLAIPWSGS"
     gene            complement(6766..6969)
                     /locus_tag="VF195_04930"
     CDS             complement(6766..6969)
                     /locus_tag="VF195_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6580197.1"
                     /translation="MTGSPSFVTGRLFTVAEVAEVMRVSNMTVYRLIRSGELPALRVG
                     KGYRIFESDLERFLEGRSVHVEG"
     gene            complement(7163..7939)
                     /locus_tag="VF195_04935"
     CDS             complement(7163..7939)
                     /locus_tag="VF195_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX6580198.1"
                     /translation="MDDAWIRAIVALPFGLVVGSFMTVAVHRLPKGESVVQPRSRCPS
                     CGAEIGARDNVPVLSWLLLRGRCRRCGQRISVEYPLLEVTTAGVVVLAAIRHPDPWQA
                     VLVGGLLALMPGIALIDLRHRIIPNRLTYPALLLFVPVILLAWAIGDAADPVRAGLGL
                     LLYGGVLFIVAAISGGMGMGDVKLAAVIGLVLGSLGLRFVGVAAAAAIVLGGLGAIVA
                     LAMGRGRKSAIPFGPYLAVGAVVAGLWGEPLASWYIGRFL"
     gene            complement(8000..9439)
                     /locus_tag="VF195_04940"
     CDS             complement(8000..9439)
                     /locus_tag="VF195_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580199.1"
                     /translation="MLTRLRDDEQGVAMIIAVSVAFVVLMLATVVVAQSIHSLDSSGY
                     DRERLLSVNAAEAGTNRWYAYLQTTSSPSMAADPGCDSSTGLLTLSDTVQSGPSAAAY
                     AAIGTFYGSDGATQMSCSTFTDTTFPSYVKVRSTGTINGAPNRTLETFVRLTPNYGGF
                     GAAILAVNGTTISNSFTISGNSGNDGDIYILSGDLVIANAMTLSGNIYVPNGSVQISN
                     GATVKGNVWADGDVMIDNPAVIQGDALSSNGDITGSGAINDDATAAGTVNTSTLTIGG
                     IVTQGVTMPAVPTQTFPQITSATAPWIASGYELVDYSANAGATNCAKARDWIKNTWPG
                     SAITNALIRIDTTCTFTNSNNDVFSIKGNLAILNDGGFNLTQRSTWNGTAGSIKNIHF
                     ISVYSATCSGTTKDISVSNFTTFNAYTNVSFYTPCRATMSNLNAFSGQVLSTDVTINN
                     NFKMNYKPVLVPGITGVTGFKQDISYIHE"
     gene            complement(9463..10104)
                     /locus_tag="VF195_04945"
     CDS             complement(9463..10104)
                     /locus_tag="VF195_04945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX6580200.1"
                     /translation="MAVMRATIRMLRSEYGMTLVELIVAMAILSIVSLVFTSTLSSVQ
                     RAVVEEDVRIRLNDQMRLAVQSIDRLVRSGNILYDPVDETGNDPYATTATGYMFRIYT
                     QAEQAASQDARCALWLVDDEERLLYRTWPILAPEDASNWTVVAEGVVNRSLGEPAFTL
                     DEAGRTIAVEFHVNPDLAHRPQATQVVEGSLTGRNTSFGYPVQLCETLPDPLI"
     gene            complement(10106..11908)
                     /locus_tag="VF195_04950"
     CDS             complement(10106..11908)
                     /locus_tag="VF195_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580201.1"
                     /translation="MSRCHPIRRIRAGVRDERGFTILETVIAIMVVFASLTALVYTAS
                     IGFRYVGYGRDRIQATGIANRVMEDIRGLAYTKITNGIPSSELTSDTRIKSCSGVYRF
                     ETCSGEKIVSSTFAPSYEAEWLVPHTDQLSVGNLDFTYHTYVTNDTPTTTPYRVTVIV
                     EWSNGAIRSAPNNSVRVQSLFWSPSGCVNPNTHPFAAPCQPFFYAQVDAPQARLDVTG
                     QLHDFAVDFDSVALTLPGISATAQEEQTTQLNAATTLSGIQFVDSAGTASDGNTQATS
                     EADDEVETSAVAASGGNSTNPTPYAASRLNADCCNQIGLVPSIPSGDQEHRATSVDAA
                     ATDAYACPTTGAREADLLPCAGGHIRQVGTITVAVPVSHVTPTVGSANLLRIVGPATD
                     TTATIDRDASAGTAEDGLIDVQATRTLGTVQLGGFPSSGMTAPANMSTNQTLDSNYCV
                     RLVGYSDTARAIAGERQGTNPSASISAGTFYYYTGSGSGYSNKSVTDATLDSLTVTNC
                     TKTQIVGLSTVTWQVTVALGGIKHATTMTSQTADPADAQTRWDAEASVQPIEITIRYQ
                     YIVDGVTEMNLLATFDPGDLFAHSIYQPPPAEQV"
     gene            complement(12067..12534)
                     /locus_tag="VF195_04955"
     CDS             complement(12067..12534)
                     /locus_tag="VF195_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018295312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX6580202.1"
                     /translation="MREREEGFTLIELMVVVLIIGILVAIALPTFLGARERAQNRAAQ
                     SSLRNALVAAKTAYTDVSDYSTATDADLGAIEPSLTYLPAATVSTGPTAVSVRVSDQA
                     VGDSQVWSAAAMSESGDCFEISDNATGPGTTFGRGAAATCSGTNAIAAAGGWA"
BASE COUNT         2003 a         4398 c         4211 g         2026 t
ORIGIN      
        1 ccgaacgcga cggtccgcgt catgtccgtg tggtagccgt cgacaagggc gccgaagtcc
       61 atcttgatga tgtcgccctc tccgagtgcc cgatgacacg gccgatggtg cggttcggcg
      121 gcgtgctccc cgaaggcgac gatcacgtcg aaggacggag cgtcggctcc gtgccgagcc
      181 atcgcgagct cgagctgcgc cgcggcctga cgctcggtga tcccgacgac gagcacctcg
      241 aggatgtcct cgaacgcgcc gtcggtagcc tcctgtgcgg ccctcagctc cgtgagctcc
      301 tcggggtcct tggaccatcg gagtcgctcg acctcttccc ccacacccgt gagccggagc
      361 ccgtcgaacc gctcctgcca tcgctcgaac tgcgcgacgg agacggcatg gcgttcgaac
      421 cccagctgct ggacgccgag tccgcggacg tgctcgagga tcgccacggc gggatcctcc
      481 atcgacgcca cgcggtcgag atccgggacc tcgtggcggg actgctcgtc gtagcgcccg
      541 tcggtgatga acaccgagcc cgccggcccc acgagcgcca tgcccgacga gcccgtgaac
      601 cccgtcaggt accggacgtt cgcgagcttg gtgacgagca gcgcgtcgat ctccaggtcg
      661 gcgcagcggt ccgccagggc gttcctcctc gtcacgtgat ccacggcatc gcctccagag
      721 ccagatgata gccaccggcc cccaggccga tgatcgacgc gcgacagact tcgctcacca
      781 cggatcgatg ccggaagtct tcccgcgcct gcgtgttcga catgtgcacc tcgatggccg
      841 gcaggccggc ggactcgatc gcgtcccgga gcgcgtagga gtagtgcgtc agggctcccg
      901 ggttgatgac gatgacgccg acgtcctcgg ccgccgcccc caggagccaa ccgaccaggt
      961 ccccctcgtg atcggactgc cgccagagga cctcgtgtcc catccctgtg gctcgctcct
     1021 cgaccgagac catgatctcc tcgaggctct ccgatccata gagggaggga tcccgccgtc
     1081 ccagcgcgcc gaggttcgga ccgaacaggt acaggacctt catgcgggtg ctcccatctc
     1141 gagcaggacg gctgccacct ccgtatcgga cacgtcgtcg acgacgatcg ggcgtcctac
     1201 gtcttcgagg agcacccatc tcaccccgtc ccggtacttc ttgtcgagac ggaggcgacg
     1261 gagcacctcg tccacgggag ggagcgcgcc gtccggggcg agcccgagcg agcccaggag
     1321 ccgctcggtc ctcgcgcgga gcccgaacgc gagcccccgt gcctccgcca gcgcggcggc
     1381 gaacaccatc ccgagggcca ccgcctcccc gtgggtccgt ccctcgaacg cgtcgaggcg
     1441 ctccagcgca tgaccgagcg tgtgcccgta gttcaggacc agccgcgcgc tcgcgtcccg
     1501 ctcgtcctcg gcgacgatcc ccgctttcgc ggccacacat cgtgcgatca cgtcctccag
     1561 gacggtcgcg ctgcgtcgaa cgatcggtcc cggatcccgc tccaaggtgg cgagcagacc
     1621 ctcgtccaga gtgagggcgt acttggccac ctcggccagt ccggaacgga agtcccgctc
     1681 ctccacggtc gcgaggacgt cgacatcggc cacgaccgcg atcggctgcg cgaacgtccc
     1741 cacgaggttc ttcccctccg gcaggttcac cgcggtcttc ccgccgatcg ccgcatcgac
     1801 ctgcgacgtc agcgtcgtag ggacctggat gaacgggacc cctcgcatgt acgtggacgc
     1861 cacgaagccg gctaggtcgc cgactgcacc gccgccgagc gcgaagatga cgtcgtcgcg
     1921 atgggcctcg cgaccggcca gttgccggag gagggacccg taggcctgca gcgacttcgc
     1981 ctcctccccc tcgggcacga gcagcagcac cgttcccaat cctctgctct ctatcgcgcc
     2041 gacgatcgcg gggtgatgga ggtccgccac ggtgcgatcc gagaccacga acgcggtcgt
     2101 cgcgcccggc aggctcggca cgtgatcggc gagggtgggc aacagtccgg atccgatcgt
     2161 cacgtcgtag gaccgcccgg gcaccggaac ggtcacacgg accaacgcag ctcctcgacg
     2221 atcgccgccg ccaccacccc cggatcgccg gtgccgtcca cgacgtgggc ggcgaactcc
     2281 cggtagagag gtccgcgcga ggccaacaat cgctcgagat ctccttcctg gcggatcaag
     2341 ggccgatccg acgcgggccg cacacggcga cgcaactcat cgagaggaac atcgaggtac
     2401 acggccacgc ccgtgttgcg tagcgtgatc cggttggccg gctcgagcac gaccccgcct
     2461 ccgcacgcga ccacggacgg atcctgcgtc gatgccttca cgagcgccgc ggcctccagc
     2521 gcccggaacg ccgcctcgcc ctcgtctcgg aagatgtcgg tgaccgccgc gccggcttcc
     2581 cgctcgatct cgccgtcgag gtcgacgaac gggaccccga gctgtccggc gagctctcga
     2641 ccgaccgtgg acttcccggc tcccggcatg cccaccaggt agacgatcac ggcagcgccg
     2701 ccacgaagcc gtcgaggttg cggcggatct caggcaacga gtcaccgccg aacttctcca
     2761 cgacggcgtt ggcgagctcg aacgcgacca cggcctcgcc gaccacgccg cccgcgggaa
     2821 cggcgcagac gtcggtccgc tgcttgatcg ccatcgcatc ctcccgggtc gcgagatcga
     2881 tcgtggctag gggcttcggc acgctggaga agggcttcat cgccgcccgc agccggatcg
     2941 gctgtccggt gctcatcccc ccctcgatcc cgcccgcccg agcggtggcc ctggcgatcc
     3001 tcccgtcgcg ccggacgatc tcgtcgtggg ccgccgagcc gtagcgtgcg gcggaggcga
     3061 acccgtctcc gaccccgacg cccttcaccg actggatgct catcaacccg agggcgagac
     3121 ggccgtcgag cttccgatcg tagtggacgt gagaaccgag cccgggcgga catccgtagg
     3181 cgatcacctc gaactctccc ccgaccgtgt cccgatcctt gcggacccga tcgatctcgg
     3241 ccaccatctt ctcggcgacg gcttcgtcga agcaccggac cggcgatgcg tccacggcct
     3301 cgaggtcctc cggtgcgggc cagcggctcg cggcgcgcac cgcgaccttc ccgatccgaa
     3361 cgacgtggga cacgatccgg atgtcgagtt cggtcaggag tgccttgcag acggccccga
     3421 tcgccacccg ggcagccgtc tcgcgggcgc tcgcgcgctc caggacgttg cgcacgtcgt
     3481 cgaagccgta cttcatcgcc ccggcgaggt cggcgtgtcc cgggcgaggc cgggtcagcc
     3541 gatcggccgg atccccctcg ccttccaggg acatcaggtc ccggtacttc cgctcccact
     3601 cggcgttcgg gatcgtgatc gcgatcggtg agccgagcgt ggtgccgtgg cggatgccgc
     3661 tcaggatgtc gagcgcatcg gtctcgagtt tctgccggcc gccacgaccg tgcccgtgcc
     3721 ggcgccgcgc gagctctgcc gcgatctgct tcggggccac cggcacggcc gccggcatgc
     3781 cttcgagaac ggcgacgaga gccttcccgt gggactcacc ggcggtgagc atcctgagca
     3841 cggaagcatc gtatcggacc ccatcggccg gcccggaaaa tgcgggggcg gcgcgagcgc
     3901 cgcccccgcg ccggcgaccg ctccccccgt gggcgggtcg ccggagctac ttctgagccg
     3961 tgatgcagag cgtgaacgat tcgtcgccga acaggaacgt ggcacagttc cccgccaccg
     4021 atcgcagctc gaacctgccc ccggcgaact gatccccgat cccgacgtcg aagaccgtgc
     4081 cgtccacctc gacctggacg cgggtcgcgc cgtcccgctc gaagatgtcg aggaggacca
     4141 cggtcgaccc gccgacgttc tggctcgagt cgttgcccgg cgccgtcggc gacggcgtcc
     4201 cggacggccc gacggacggg ctcccggacg ggctcccgga cgggcccccc gatggccctg
     4261 gagccgtggg aagcggagaa ggcgacagag cgggcggaac ggagaaggga tcccgcccgg
     4321 tgaagctctg gatcggaggg cctccgtccg gaggcggact cggggagctc gggggcagcg
     4381 gggtgtccgg gatcggcggg aacggctcct cgccgccgcc gagaaccgag aagaggacga
     4441 accccgcgag gaggacaccg acgacgctgc cgccgatcgt gacggtccgg cgatcccgtt
     4501 cagataacgc catcgttcat cctcccgcga ccgcgacggt ctctgtcgga ccgggctccg
     4561 agccgggtcc ggcgctcagg tcgctcgtgt acaacgtgat cgtcgccgtg aacgagagcg
     4621 tggacccctc ggtcggcgtg aggttgatcg cttcgacggt ggcagcgcga ggcagcgtct
     4681 cgatctcgtg caggtacttc accatcgcga agtaggaaca ctcgccactc gccgagaccg
     4741 agatggtcga gaacccgccg gtcgtcgacg ccacgggcgg cgccggcgtg aggctcacca
     4801 cctgcacacc ggccaccgag gcggcgttgc ggaggaagag gatcaccgcc gacaggtcgg
     4861 ccgtgggagg cacctgctgt tcgatccgtc ggatcgtcgc ctcgttcaag ggggcttcat
     4921 cgcgcgcctg ctcgagggcg ttcaatcgag cctgcagcac ctgctgctcc gcggccgtgt
     4981 cctggagctc cttctccgcc tccgtgacct ggcccatctt cgggaggacg aggaagacga
     5041 tgaggatcag ggcgaggatg gccgcgcccg ccccaacgac gatgatggcc cgcctaccct
     5101 gcatcggccg tcccacctgc ttgctggccg cgctccgtga ccacggccgt tgtgaggtcg
     5161 accccggacg cgtacgtgat cggtcccgtt tccgggtcgg cgatcgaggt cacccacggg
     5221 ttcacccagc cgcggatccg ctccaggcgc atcagccagg acgcgatcgt gtcgtagtcg
     5281 atggcaaggc cggccacgtc gatcctgccc gtgagcagcg tgctcccgcc agtggtcacg
     5341 gtgggctcct gggccgtcgc ggagaacgac gtcagcgccg cgtcgctggg gatgaccctg
     5401 gagacatcca tcaggatgcc ggagaaggag atctcgttcg cgtagacctg gttcaggagg
     5461 tcctgcttcg cctgggcccg gctacggatc tcctcgaact gctggagcgc ctcgatgtcc
     5521 cgctgcaggc cggcgttgtt ctgctcggcc ctctcgatgt cctcacgcac gctgcccagg
     5581 gagccgagct ggaggacgta gaacatgaag atcagcccga tcacaccggc gccggcgagc
     5641 gccacgaccc ccgccaaccg gcgctgtcgc tgggcggcga ggatgtcggg cgggaggagg
     5701 ttgacctgac tcacgagccg ctccagggga tggcgagacc gacggcggtg gcgagcacgg
     5761 gctccgcctc cgcctgcgcg tcctcggaca acgagagctg cgaacccacc ttgctgaaga
     5821 cccggcccgg ttccacggtc accgggatgc gctgacgcac gatgtcgatg aatccctcga
     5881 gcttggatcc gccgccggag atgagcagac gctcgatccg cgctccttgc gtctgagctg
     5941 tgtagaactc cagggacgag cggatctcgt ccacgaactg cgtcgcacgc tgcatggcga
     6001 cctggagcgc ttgctccggc gtcgcgtccg acccttcgac cagctcgccc cgcttcaacc
     6061 gctccgcgac ctcgtcctcg acggagagcc cgcgggcgat cgccacggtg atgtctcgac
     6121 ccccggacgg caggatccgc acgaaccggg tctcgcccgc ctcgtggacc acgatgttgg
     6181 tgacgtgggc gccgacgtcg acgacggcct cgcccccgga gcgctcgagc tccatccccg
     6241 cgccggtcgc gccgacggcg cgcaccatcg cgaacgggac gagatccagt cccatcggct
     6301 cgagcttcgc cttcgtggcc gaggtgacca aggtgtcgac catcgccttc tgggcggcca
     6361 ccaggaggag acggagcatc tggcgtccgt cgacctcgat ctcctcgatc aggtggtagt
     6421 cgagaaccgc ctcgtcgacc ggcatcggga tgaactcctg cacctggaac cccagtgact
     6481 gccgcagctc cttctcgggc atcgtcggaa gcgcgatctc gcgaacgacg acgcgctggt
     6541 tccccacgcc catccacacc ttgcgggact tgaatccgcc gcgcgtccag agctcgcgga
     6601 gcgattccga cacggcctcc acctcccgga cctcgccgtt ctcgacggct ccggcgggca
     6661 gcggcacctg ggcggccctg accacggttg gcggggatcc ctccgccagc tccgcagcgc
     6721 gaacggcggt cgaacccacg tcgagcccga tccttgtctt cggcactagc cctccacgtg
     6781 gacggatcga ccttcgagga agcgttcgag atccgattcg aagatccggt agcccttccc
     6841 gacccggaga gccgggagct cgcccgagcg gatgagccgg tagaccgtca tgttcgagac
     6901 gcgcatcacc tcggccacct cggccacggt gaacaggcgc cccgtcacga acgatggtga
     6961 accggtcacc agacccctct cctccgggag tgtctcatgt gccacatgaa gcacactagt
     7021 aacattgagt gctcatgaag cacacctgcc tctttgagga tgctcaggaa acaccaggca
     7081 acacgccaag tcaaacatct ccggcgtcag ggtccggagt cttccgacgc gctccgagga
     7141 ccgtcggagg agtaaccctg gatcagagga agcggccgat gtaccaggag gcgaggggct
     7201 cgccccacaa gccggcgacc accgctccca cggccaggta cggaccgaac gggatcgcgc
     7261 tcttccgccc cctccccatc gcgagcgcca cgatcgcgcc gagcccgccg agcacgatcg
     7321 cggcggcggc cgccacgccc acgaagcgaa gcccgagaga tccgaggacg aggccgatca
     7381 ccgcggccag cttcacgtct cccatcccca tcccccctga gatcgccgcc acgatgaaga
     7441 gcaccccgcc gtagaggagg agtccgaggc cggcccgcac gggatccgcc gcatccccga
     7501 tggcccacgc gagcaggatc acgggcacga acaggagcaa cgccgggtag gtgagcctgt
     7561 tcgggatgat ccggtggcgg agatcgatca gcgcgatgcc cggcatcagc gcgagcaggc
     7621 cgccgacgag cacggcctgc cacggatccg gatggcggat cgcggcgagg accaccaccc
     7681 ccgcggtggt gacctccagc agcggatact cgaccgagat gcgctggccg catcgacggc
     7741 atcgcccgcg gagcaggagc cacgagagca ccggaacgtt gtcgcgggcc ccgatctccg
     7801 cgccacacga ggggcagcgg gatcgtggtt gcacgaccga ctcgcccttc ggaaggcgat
     7861 ggacggcgac ggtcatgaag ctccccacca ccaggccgaa gggcaaggcg acgatcgcgc
     7921 ggatccacgc atcgtccacg aggccgaacc ctacctccgt tcggtccggg ggtggggaac
     7981 ccccggaggt cgcaggcgct cactcgtgga tgtaggagat gtcctgcttg aaccccgtca
     8041 cgccggtgat ccccggcacc agcaccggct tgtagttcat cttgaagttg ttgttgatcg
     8101 tgacgtccgt cgacaggacc tggccggaga aggcgttcag gttgctcatc gtggcccggc
     8161 acggcgtgta gaacgacacg ttggtatacg cgttgaaggt cgtgaagttg ctgaccgaga
     8221 tgtccttggt cgtcccgctg caggtcgccg agtagacgct gatgaagtgg atgttcttga
     8281 tcgaacccgc ggtcccgttc cacgtagacc gctgggtgag gttgaagccg ccgtcgttca
     8341 ggatcgcgag gttccccttg atcgagaaga cgtcgttgtt ggagttcgtg aatgtgcacg
     8401 tcgtgtcgat ccggatcagc gcgttcgtga tcgcgctccc cggccatgtg ttcttgatcc
     8461 aatcacgagc cttcgcgcag ttcgtggcgc ccgcgttcgc gctgtagtcg acgagctcgt
     8521 acccgctcgc gatccatggc gcggtcgcgg aggtgatctg cgggaatgtc tgcgtcggga
     8581 cggccggcat cgtcacgccc tgcgtcacga tgccgccaat cgtcagagtc gaggtgttga
     8641 ccgtcccggc ggccgttgca tcgtcgttga tcgccccgct cccggtgatg tcgccgttgg
     8701 acgagagcgc gtcgccctga atcacggccg ggttgtcgat catgacatcc ccgtccgccc
     8761 acacgttgcc cttcacggtc gccccgttgg agatctgcac cgacccgttc ggaacgtaaa
     8821 tgttccccga cagggtcatc gcgttcgcga tcaccaggtc gccgctcagg atgtagatgt
     8881 cgccgtcgtt gccgctgttg ccgctgatcg tgaagctgtt cgagatcgtc gtgccgttca
     8941 cggccaggat cgctgctccg aaacccccgt agttcggcgt cagtcgcacg aaggtctcga
     9001 gcgtccggtt cggcgccccg ttgatcgtgc cggtcgatcg gaccttcaca taggagggga
     9061 acgtcgtgtc cgtgaacgta gagcagctca tctgggtggc tccgtcggag ccgtagaaag
     9121 tgccgatcgc ggcgtaggcg gccgccgacg ggccggactg cacggtgtcg gagagggtaa
     9181 gcaacccggt ggacgagtcg cacccggggt cggccgccat gctcggcgac gaggtggtct
     9241 ggagatacgc gtaccaccgg ttcgtccccg cctccgcggc gttcacggag agcaggcgct
     9301 cgcgatcgta cccgctggaa tcgagcgagt ggatcgactg ggccacgaca accgtggcca
     9361 gcatgaggac tacgaacgcg acggagaccg cgatgatcat cgcgactcct tgttcgtcgt
     9421 ctctcagtcg ggtcagcatc tcgatccttt cagcttgtgg tgtcagatga gggggtcggg
     9481 caaggtctcg caaagctgga cggggtagcc gaaggaagtg ttccgccccg tcagcgatcc
     9541 ctccaccacc tgcgtggcct gcggtcgatg ggcgagatcc ggattcacat ggaactcgac
     9601 ggcgatcgtg cggccggcct cgtccagcgt gaaggcgggc tcgccgagcg agcggtttac
     9661 cacgccctcg gcgacgaccg tccagttcga ggcgtcctcg ggtgcgagga tcggccacgt
     9721 gcggtagagg aggcgttcct cgtcgtccac gagccacagg gcgcaccgag catcctggct
     9781 cgcggcctgc tcggcctgcg tgtagatccg gaacatgtag ccggtggctg tggtggcgta
     9841 cggatcgttt cctgtctcgt cgacagggtc gtagaggatg ttgccggatc ggaccaaccg
     9901 gtcgatcgac tgcaccgcca aacgcatctg gtcgttcagc cggatgcgca cgtcctcctc
     9961 cacgacggcg cgctgcacgc ttgacagcgt cgaggtgaag accaaggaca cgatcgacag
    10021 gatcgccatc gccacgatga gctcgaccag cgtcatgcca tactcgctcc ggagcattcg
    10081 gatcgtcgcg cgcatcacgg ccatctcata cctgctcggc cggcggcggc tggtagatgc
    10141 tatgagcgaa caggtcgccg ggatcgaacg tcgccagcag gttcatctcg gtgacgccgt
    10201 cgacgatgta ctggtaccgg atcgtgatct cgatcggctg gaccgacgcc tcggcgtccc
    10261 atcgtgtctg cgcgtccgcc gggtccgcgg tctgcgaggt catcgtggtg gcgtgcttga
    10321 tgccgccaag cgccaccgtg acctgccacg tgaccgtcga gagtcccacg atctgcgtct
    10381 ttgtgcagtt ggtcaccgtg agcgaatcga gcgtcgcgtc cgtgaccgac ttgttggaat
    10441 acccgctccc agaccccgtg tagtagtaga acgtgccggc cgagatggac gccgacgggt
    10501 tcgtgccctg gcgctcgccc gcgatcgcgc gggccgtgtc cgaatagccg acgagtcgga
    10561 cgcagtagtt ggagtcgagc gtctggttcg tggacatgtt ggccggagcc gtcatgccgg
    10621 aggacgggaa cccgcccagc tgcacggtcc cgagcgtgcg cgtcgcctgg acgtcgatca
    10681 atccgtcttc ggcggtgccc gcagatgcgt cccgatcgat cgtagccgtc gtgtccgtgg
    10741 ccgggccaac gatacggagc agattcgccg agccgaccgt aggcgtcacg tgggacacgg
    10801 ggacagcgac cgtgatcgtt ccgacctgac ggatgtgacc cccagcacac gggagcaggt
    10861 ccgcctcccg cgcacctgtg gtcgggcacg cgtacgcgtc cgtggcggcc gcgtccaccg
    10921 acgtcgcacg atgctcctgg tccccgctcg ggatggatgg gacgagtccg atctggttgc
    10981 agcaatcggc gttcaggcgt gaggcggcgt agggagtcgg gttcgtcgag ttcccaccgc
    11041 tggccgcgac cgcgctcgtc tcgacctcgt cgtcggcttc cgatgttgcc tgcgtgttgc
    11101 cgtcgcttgc cgtccccgcg ctgtccacga actggatccc ggagagcgtc gtggccgcgt
    11161 tgagctgcgt cgtctgctcc tcttgcgccg tcgctgagat gcccgggagg gtgagcgcga
    11221 ccgagtcgaa gtcgacggcg aagtcgtgga gctgcccggt gacgtccagt cgcgcctgcg
    11281 gcgcgtcgac ctgggcgtag aagaagggct ggcagggggc ggcgaacgga tgcgtgttgg
    11341 ggttgacgca tccgctcggc gaccagaaga ggctctggac gcggaccgag ttgttcgggg
    11401 cgctcctgat cgctccgttc gaccactcga cgatcaccgt cacgcggtac ggggtggtgg
    11461 tcggcgtgtc gttcgtgacg tacgtgtggt acgtgaagtc gaggttgccg acggacagtt
    11521 ggtcggtgtg tgggacgagc cactcggcct cgtagctcgg ggcaaacgtg gacgagacga
    11581 tcttctcgcc ggagcacgtc tcgaaccgat acacgcccga gcaactcttg atcctggtgt
    11641 cggaggtgag ctcgctcgag ggtatgccgt tcgtgatctt cgtgtaggcg agaccgcgga
    11701 tgtcctccat cacgcggttg gctatgccgg tcgcctggat ccggtcgcgc ccgtagccga
    11761 cgtagcggaa gccgatcgac gccgtgtaca cgagggcggt gagcgacgcg aaaacgacca
    11821 tgatggcgat gaccgtttcc aggatggtga atccgcgctc gtcgcgcaca cccgcacgga
    11881 tccgccggat gggatgacac ctgctcatgc tccgcccttc gaagagtcct tccgttaggg
    11941 attcggcatg ctcgatgccc cccttgaggg tcggctggct ctgcgccgta cccgatatgg
    12001 ggtgagatgc ggaacgggcg gccccgaggg gccgcccgtt cagccttccg atcgtgcgtc
    12061 gtcggcttac gcccaaccac cggccgcagc gatcgcgttg gtgccggagc acgtggcggc
    12121 cgcaccgcgg ccgaacgtgg tcccgggacc ggtcgcgttg tccgagatct cgaagcaatc
    12181 cccggactcc gacatcgcgg ccgcggacca gacctggctg tcaccaaccg cctggtcgga
    12241 cacgcggacg ctgaccgcgg tcgggccggt cgacacggtg gccgcgggca ggtacgtcag
    12301 cgacggctcg atcgcgccga ggtccgcgtc ggtcgcggtg ctgtagtcgg agacgtccgt
    12361 gtaggccgtc ttcgcagcca ccagggcgtt gcggaggctc gactgagccg cccggttctg
    12421 cgcccgctca cgggcgccga ggaacgtcgg cagagcgatg gcgaccagga tgccgatgat
    12481 cagaacgacc accatcaact cgatgagggt gaacccctcc tcacgctcac gcatacgctt
    12541 gagcattctg ccttcgcctc ccttcctttg agtcggcgca ccacagcggt gcgcgttccc
    12601 cactgatttc ggcgtccccc ggcccgaact gaagcccc
//
LOCUS       DASUYD010000081         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_41524, whole genome shotgun sequence.
ACCESSION   DASUYD010000081 DASUYD010000000
VERSION     DASUYD010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41524"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF195_04960"
     CDS             <1..732
                     /locus_tag="VF195_04960"
                     /inference="COORDINATES: protein motif:HMM:NF014186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX6580203.1"
                     /translation="GQPDSPPIETQAQIDALDLPGPTSGTSFLGMNLLCSAVHAGNTD
                     ATLAAQSPTGQQLEYPVNCGSDVVDRIPYYVFAVLMFATTFFQQRQMQNASPPGASSQ
                     QQAVLKLMPILFGVFGVFFPAGLVLYWTTSNAWQIGQQYFMLKSRPTAEQMAERAKAK
                     PQKVEKKGFMASMMERAEQQRQQRGSATKPVAKKPGSSPKPGSSPKPGSTPKPGSNPK
                     PGSNPKQSGSGGNGSGGDPKKRPKR"
     gene            698..1366
                     /gene="jag"
                     /locus_tag="VF195_04965"
     CDS             698..1366
                     /gene="jag"
                     /locus_tag="VF195_04965"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6580204.1"
                     /translation="MEEIRRSAQSVEEAVEAALGELGLTEQEVDVDVVQEPRPGVFGV
                     GAQDAIVVVRSRAKPAGDVGEDELDEQAEIAAEFLEGMLERMGITATVEPAIEDGTMY
                     VDVIGPEPDDEDMGLLIGRHGQTLEAIQELTRVAIGQRTGLPSRVVVDIEDYRKRQIE
                     RLSARVREIAVRVSRTGRAETLEPMNPFLRKMVHTAVAEVDGVDSSSEGEGADRRVVI
                     SRAR"
     gene            1414..2022
                     /locus_tag="VF195_04970"
     CDS             1414..2022
                     /locus_tag="VF195_04970"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX6580205.1"
                     /translation="MSEPEILLGQAAEHGVTLSAWQAATLLRYEDLLARRAVPLGAVA
                     RSDATRIRDRHILDCLRAAPLVHEDDVACDLGSGAGLPGIVVAVAEPLVQVLLVESRA
                     KRAAFLEFAVQELRLSNAQVVVGRAEELRRRVDACFARAFAPLPRAWSVARGILRPGG
                     RLIYFAGVELGDPAVPYGAALEAVVRTAVLESAGPLVIMTCQ"
     gene            2196..2942
                     /locus_tag="VF195_04975"
                     /pseudo
     CDS             2196..2942
                     /locus_tag="VF195_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007409899.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
BASE COUNT          639 a         1183 c         1237 g          529 t
ORIGIN      
        1 gggcagccag actcgccgcc gatcgagacg caggcccaga tcgacgcgct ggacctgccg
       61 ggtcccacgt cgggaacctc cttcctcggg atgaacctgc tctgctccgc cgttcacgcg
      121 gggaacacgg acgccaccct cgccgcgcag agccccaccg ggcagcagct cgagtacccg
      181 gtcaactgcg gcagcgacgt ggtggatcgc atcccctact acgtgttcgc cgtgctgatg
      241 ttcgccacca ccttcttcca gcagcggcag atgcagaacg ccagcccgcc gggcgcctcg
      301 tcccagcagc aggcggtgct caagttgatg ccgatcctgt tcggcgtgtt cggcgtcttc
      361 ttcccagccg gtctggtcct gtactggacg acctcgaacg cttggcagat cgggcagcag
      421 tacttcatgc tcaagagccg tccgacggcg gagcagatgg ccgaacgagc gaaggcgaag
      481 cctcagaagg tcgagaagaa gggcttcatg gcttcgatga tggagcgcgc ggagcaacag
      541 cgccagcaac ggggctcggc gaccaagccc gttgccaaga agccggggtc cagccccaag
      601 ccggggtcca gccccaaacc gggatccacc ccgaagccgg ggtcgaatcc gaagccgggg
      661 tcgaatccga agcagtcagg gtccggaggc aacggcagtg gaggagatcc gaagaagcgc
      721 ccaaagcgtt gaggaggccg tcgaggccgc cctcggcgag ctcggcctga ccgagcagga
      781 ggtcgacgtc gacgtcgttc aggagcctcg tcccggggtc ttcggggtcg gagcgcagga
      841 cgccatcgtg gtcgtccgct cgcgagcgaa acccgcgggg gatgtcggcg aagacgagct
      901 ggacgagcag gccgagatcg ccgcggagtt cctcgagggc atgctcgaac ggatgggtat
      961 cacggcgacc gtcgaaccag cgatcgagga cgggaccatg tacgtggacg tgatcggtcc
     1021 cgaacccgat gacgaagata tggggctcct catcggtcgg cacgggcaga cgctcgaggc
     1081 gatccaggag ctgaccaggg tcgcgatcgg ccaacgcacg ggcctcccct cccgggtcgt
     1141 ggtcgacatc gaggactacc gaaagcgaca gatcgaacgg ctttcggcgc gcgtgcgcga
     1201 gatcgccgtg cgcgtctccc ggacgggccg agcggagacg ctcgagccga tgaacccgtt
     1261 cctgcggaag atggtccata cggcggtggc cgaggtcgat ggggtcgaca gctcgagcga
     1321 gggcgaagga gctgatcgac gcgtcgtcat cagccgagcg aggtagccgg acggagcgac
     1381 ggccatcaag gtgccgtttc acgtgaaaca tcgatgagcg aaccggagat cctgctgggg
     1441 caagccgcgg agcacggagt tacactcagc gcctggcagg ccgctacgct cctccggtac
     1501 gaggatctcc tggcccgtcg agcggtcccg ctcggtgccg tcgcgcgttc ggacgcgacg
     1561 cggatccggg atcgccacat cctcgattgc ctccgcgcag cgccgctggt gcacgaggat
     1621 gacgtcgcct gcgatctggg gtccggagca gggctgccgg ggatcgtcgt ggccgtcgcg
     1681 gagcctttgg tccaggtcct gctcgtcgag agcagagcca agcgagccgc tttcctcgag
     1741 ttcgccgtcc aggagctgcg actgtcgaac gctcaggtgg tggtgggacg agccgaggag
     1801 ctgcgtcgga gggtcgatgc ctgcttcgct cgggcgttcg ccccccttcc gcgggcctgg
     1861 tcggtagctc ggggcatcct tcggccgggg ggtcgcctca tctacttcgc cggcgtcgag
     1921 ctcggggacc ccgcggtccc ctacggggcc gcgctggaag cggtcgtacg cacggccgtg
     1981 cttgaaagcg ctggtcccct ggttatcatg acttgtcagt gacgaagccc tcctcttccc
     2041 gggcagctcg ttcgtcctcc aagcccaagc cggtatccaa acgagcaagc caggcttcgg
     2101 ggacgcaacc gactgccgtg caacgttctt cgaaggcgcc gcgcgaggcg ccggagactt
     2161 cggactccgc tccagccgcc tctcgtgcgg cacggatcat cgcggtcgcg aaccagaagg
     2221 gcggtgtcgg caagagcacg acggccgtga gtctgggagc gtcgctcgcg gagctcggcc
     2281 accgggtcct ggtcgttgat ctggaccccc aggggaacgc ctccacgggg ctcggcatcc
     2341 gacacgagca gcgcgaggtc acggtgtacg acgtgctcgc cgacgaggct ccgatcgagc
     2401 agtcgatcgt ccccacgccg atcgacaacc tgttcgcgat cccctcgacc atcgaccttg
     2461 cgggcgccga gatcgagctg gtgagccagt tttcacgcga gctccggctc aagaaggcca
     2521 tcgagcccct gaagcacgac gcctacgact acgtcttcct cgattgtccg ccgtcacttg
     2581 ggctcctcac agtcaacgcc ctcgctgccg cggaagagct catcgtgccg atccagtgcg
     2641 agtactacgc cctcgaaggc ctgggacagc tcctgcgcaa cgtccgcctc gtgcagcaga
     2701 acgtcaacac gggcctccgc ctgaccggca tcgtgatgac gatgttcgac ccgagaacca
     2761 agctgtccga gcaggtggtt gccgaggtcc ggcgctactt cggcgatcgc gtctatcaga
     2821 cggtcatccc gcgtaccgtc aggctctccg aggcgccagg attcgggcag ccgatcaacg
     2881 tctacgaccc gacctcgaag ggcgccagga gctatcgcga tctcgcccgg gaggtggctg
     2941 cacgcctccc cgacgagcga ccgctcccga acgtggacga ggtgccgagc gtcacgatcc
     3001 cccgccccga gcagccaccg agccgggcgc cgcgtccgtt ggtggaaccc gagcccgagg
     3061 cacctccgga gaagagcgag gcgcgcgtct cttccctcga gccggaagca gtgcctcccg
     3121 agggagcctc ggagccgttc gttccgccgg aagaagccga accagagcct cgagcggccg
     3181 cacgcatgga gcaggcgacg cctcgtcctg cacgcgaaca ggaggatgac gtcctcgccg
     3241 gagatccgat cgagaagcaa cctgcaccac gaacccagcc cgcggaccct ggatccgaac
     3301 ccgatggcga ggtcggccgg ccgggactgg aggtggaggt gcccgggtcg aggcatgacc
     3361 agtacgagcg tgcgcctgaa gcggagccaa cgctcgacga agggtcaagc tatggggagc
     3421 cgaccgtcgc gggcatgaac ggagatgaga cgcagtctga acgagaggac gagacgcagc
     3481 tcgatcaagg aggagtcgag gccgggagga agcgccggcg gggcctgttc cgtagaggag
     3541 gatcttgatg aaacgaggag ggctaggtcg tgggctttcc gcgctgct
//
LOCUS       DASUYD010000082         5212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_264052, whole genome shotgun sequence.
ACCESSION   DASUYD010000082 DASUYD010000000
VERSION     DASUYD010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264052"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VF195_04980"
     CDS             <1..579
                     /locus_tag="VF195_04980"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEX6580206.1"
                     /translation="FNSYGARRGNHEVMMRGTFANVRLRNDLAQDREGPWTTHLPDGE
                     VTTVFDAAMRYQEERVPLVVLAGKEYGSGSSRDWAAKGPALLGVRAVIAESFERIHRS
                     NLVGLGILPLQFLPGESASGLGVTGLETFDVLGISDGIEPGQRVRVVARGTAGEEVTF
                     GAILRIDGMAEVEYYRHGGILQMVLREMLAGT"
     gene            complement(580..1104)
                     /locus_tag="VF195_04985"
     CDS             complement(580..1104)
                     /locus_tag="VF195_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX6580207.1"
                     /translation="MTVLVTYATKHGSTREIAEAVATTLVDRGIPTEALAIDTVADVG
                     QYEAVVLGSAVYMDRWLREATEFARRHREQLTARTLWLFSSGPLGTEVVDAEEQPKEL
                     AELRQTLGPRDHRLFDGALTRDALGFRERMIMKAVKAPEGDFRDWDDIRAWAITIAAE
                     LALDARAQERGGGS"
     gene            1222..1650
                     /locus_tag="VF195_04990"
     CDS             1222..1650
                     /locus_tag="VF195_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580208.1"
                     /translation="MKNSVVRAIETPEATTAIVRHHSAWPNSPRAAMTNSTAAAIASE
                     GRQRPGVAQQPGVSGVEGRLLAIGRFRHRPDRDVDEAVSRQEADEEDAPHRLLLACAR
                     DVMEGNPPCEDPLTCAEAAEPRSRVENLRTWEGSIGRHRA"
     gene            complement(1746..2141)
                     /locus_tag="VF195_04995"
     CDS             complement(1746..2141)
                     /locus_tag="VF195_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020876720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6580209.1"
                     /translation="MKASDIMRRPVVAAIPTATARDVAIQLLMGEFSGVPVAQSDGSV
                     VGVVTELDLIRAVLRAGKALETTLAQDIMTRDVTTVDAETPMEEIMKVLEEKNIIRVP
                     VTSQGKLVGVVSRSDILRAMIEPTFLRFA"
     gene            complement(2329..2766)
                     /locus_tag="VF195_05000"
     CDS             complement(2329..2766)
                     /locus_tag="VF195_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580210.1"
                     /translation="MDGCTRGVLVVEPDPTERERLAAALEGDGFRVTLCSGPTEPDYM
                     CIGARSGTCPLATEESVVVLDMSLDSETVMMGTPAEDLLGMYLEAGHPVVALASPPRE
                     EEPGRLVWLRRHPEKADLMAAVWRLASPRGVARARGTERDVRP"
     gene            3695..3970
                     /locus_tag="VF195_05005"
     CDS             3695..3970
                     /locus_tag="VF195_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6580211.1"
                     /translation="MRNGPVEQEVGAMTTLTVDKRIETRFMLLPVPLMSVIVAIRAGE
                     VGDVIEVLCADPLSTTDIPAWLRKAGHELVETAEEDDYTRFVVRKTR"
     gene            4079..4543
                     /locus_tag="VF195_05010"
     CDS             4079..4543
                     /locus_tag="VF195_05010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580212.1"
                     /translation="MDQRLGSPRWAARYGRIALVFVALATLAACNGDGGGTVDVSLQE
                     FSIAADPATVDAGEVVFEATNDGPNDVHEFVVFRTDLDPTALPTDENGAVVETGEGIE
                     LIGEIEDIPVGETQSVTLDLEAGNYVLICNIFDEDEQEAHYQEGMRTTFTVT"
     gene            complement(4864..5121)
                     /locus_tag="VF195_05015"
     CDS             complement(4864..5121)
                     /locus_tag="VF195_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580213.1"
                     /translation="MTSAPAPGSVDLYWIPLGAGGHVVRWNGKVYGSWLLARAGVETE
                     QIQPPPGGRAPGWDAGLVVAGREMGPPWTSLPMDNRAPGPS"
BASE COUNT          809 a         1769 c         1773 g          861 t
ORIGIN      
        1 ttcaactcct acggcgcccg gcgcgggaac cacgaggtga tgatgcgggg cacgttcgcg
       61 aacgtccggc tccgcaacga cctcgcccag gatcgagagg gtccgtggac gacgcatctg
      121 cccgacggcg aggtgacgac cgtcttcgac gcggcgatgc gctaccagga ggagcgggtg
      181 cctctggtcg tcctggccgg gaaggaatac ggcagcggct cctcgcggga ctgggccgcg
      241 aaggggccgg cgctcctcgg cgttcgcgcg gtgatcgccg agagcttcga gcggatccac
      301 cgcagcaacc tcgtcgggtt ggggatcctc ccgctgcagt tcctgccggg ggagtcggcc
      361 tcgggtctcg gggtgacggg cctcgagacc ttcgacgtgc tcgggatctc cgatgggatc
      421 gagcccgggc agcgggtgcg ggtggtcgca cgcgggaccg cgggtgagga ggtcacgttc
      481 ggcgcgatcc tacggatcga cggcatggcc gaggtcgagt actaccgtca cggcggcatc
      541 ctccagatgg tgctccggga gatgctggcg ggcacgtgat caggaaccgc cgcctcgttc
      601 ctgggccctg gcgtccaggg cgagctcggc cgcgatcgtg atcgcccacg cccggatgtc
      661 atcccagtcc cggaagtctc cctcgggcgc cttgacggcc ttcatgatca tgcgctcgcg
      721 gaaccccaac gcgtccctgg tgagcgcgcc gtcgaacagc cggtggtctc gcggtccgag
      781 cgtctgtcgc agctcggcga gctccttcgg ctgttcctcc gcgtcgacga cctcggtacc
      841 gagcggaccg ctgctgaaca accagagcgt acgcgcggtc agctgctccc gatggcgccg
      901 cgcgaactcg gttgcctccc tcagccaccg gtccatgtac acggcgctcc cgagcacgac
      961 ggcttcgtac tgccccacgt cggcgacggt gtcgatcgcg agcgcctcgg tcgggatgcc
     1021 gcggtcgacg agcgtggtgg cgacggcctc cgcgatctcg cgagtggaac cgtgcttggt
     1081 cgcgtacgtg acgagaacgg tcatctcgat cgcctccccg ggcagttcat gcgcccacga
     1141 gggtctggga cggccacgcg aaccagacga tgccggccat gatgcctgcg cttcagaccc
     1201 caacccggga gcagccacct gttgaagaac agcgtcgtga gagcgatcga gacgccggag
     1261 gcgaccacgg ccatcgtccg ccaccactcc gcgtggccga acagccctag ggccgcgatg
     1321 acgaacagca ccgccgcggc gatcgcgagt gagggccgcc agcgtccggg cgtcgcccag
     1381 cagccaggag tgagcggcgt cgaagggcgg ctccttgcca tcggccggtt tcggcatcga
     1441 ccagaccgcg atgtggacga ggccgtgagc cgccaagaag ctgatgaaga ggatgcgcca
     1501 catcgtctcc tccttgcgtg tgcccgcgac gtcatggagg ggaacccgcc gtgcgaggac
     1561 cccctgacgt gtgcggaggc cgcggagccc cgctcccgcg tcgagaacct gcgcacgtgg
     1621 gaggggagca tcggccggca ccgagcgtga cggagcatcc gcccgacagc ttccgttgat
     1681 ccggcaggcg tcgccactcg atccgtgact cagaacgagc ctgcggcagc ggatcgccga
     1741 cgacctcagg cgaaccgcag gaacgtgggt tcgatcattg ccctcaggat gtcggaccgc
     1801 gagacgaccc ccacgagctt gccctggctg gtcaccggga cccggatgat gttcttctcc
     1861 tccaggacct tcatgatctc ctccatgggc gtttccgcgt ccacggtcgt gacgtccctg
     1921 gtcatgatgt cctgggcgag cgtggtctcc aatgccttcc cggctcgcag gacggctcga
     1981 atcagatcga gctcggtgac gaccccgacc acgctcccgt cactctgagc aactggaacc
     2041 ccgctgaact cacccatgag gagctggatc gcaacgtccc gagcggtagc ggttgggatc
     2101 gccgctacca ccggccggcg catgatgtcg gaggccttca tgtcggtcct ctttcttcta
     2161 acgcgacaac ccatctatat ccggggggtc ctatcagcgg gagggccgtt cggctcgccg
     2221 tctataggtc cggcgaccta acggcttggc ggctcgccac ttcttcgtcc aggcctcgac
     2281 gtgtctacgg gtcccgccgg tcggattggt cgttcgggag cgaggtcgtc agggccggac
     2341 gtcccgctcg gttccccggg cgcgcgccac gccgcggggc gaggccaacc gccagacggc
     2401 ggccatgagg tccgccttct cgggatgtcg acggagccag accagcctcc ccggttcctc
     2461 ctcgcgcgga ggtgacgcga gggcgacgac cgggtggccg gcctccaggt acatcccgag
     2521 caggtcctcc gcgggcgtcc ccatcatgac cgtctcgctg tccaggctca tgtccaggac
     2581 gacgacggac tcctcggtcg cgagcggaca ggtgccgctc cgcgcgccga tgcacatgta
     2641 gtcgggctcc gtagggcccg aacacagtgt gacccgaaag ccgtcgccct cgagcgccgc
     2701 ggccaggcgc tcgcgctcgg tggggtccgg ctctacgacc agaacacctc tcgtgcatcc
     2761 atccatgcgt ggagcatcgc cgcacgatgc gtgatcgtcg cctgccgagg gtcccctttg
     2821 ctgcgggacg aatgtccgtg gggtcgtcgc ctcccagcct tcatcgtttg tcggcatgtt
     2881 cgtgcgctat tacctggaga tcgtcgcccc ccttcgagga cgtcgaggcg gcgccctcga
     2941 cgacccgcag acctgggtac cggccccctg cgagacgccg aggaccgtgg acggggcctg
     3001 ctcaccgagg tgggcctcgc ggtcgacact cgacggatcg acaaggtcga gatcgcgatc
     3061 ggccgttcct accaggcccc tcgatgcgac cgtaccgccc ctcgacgacc ttgtttgccg
     3121 atgacctggc gatcgaccgg gtaccagtgg ctgttccccc acctcgagac ggacatcgag
     3181 atagcgccgc tcggcgcgga ccagactcac ctgtcgatct gcgggcgcta cgagcccccc
     3241 gtgggtgcct tcggccggat attcgatcct acgctgctcc atcgcgtggc cgaggcgacc
     3301 gtcaagattt actcgaccgc gtggccgagc gcatccgaac gcgcgagccg gcgacgacct
     3361 gagcggtacg atcccagcgt gcctgaggtt tccctccatt ctgggaggtc gcgcggccct
     3421 cgtgtccgac ctaatcgtga tggtgaacga gggcctctcc gacttcttgg ggctcctact
     3481 tgggagcgtt gcacacaagg tcatccgtta cgccgacatc cgtcagcggt cacggtccag
     3541 gcggtgctct tcgccggggg ccgagggtcc cgcacaacct cggtcgttcg gcactgaggc
     3601 ctcgcgctca cggctgcgac gctgggtcga gagaggggcg cggaatggac gactcgcctt
     3661 cctcgatggt gctgtttctc cgagactcgg cgacatgcgc aacggccccg tggaacagga
     3721 ggtgggggcg atgacgacgc ttactgtgga caagcggatc gagacgcgct tcatgctgtt
     3781 gcccgtcccg ctgatgtcgg tgatcgtcgc gatccgggcg ggggaggtcg gcgacgtgat
     3841 cgaggtgctg tgcgccgatc ccttgtcgac gaccgacatc ccagcgtggc ttcgcaaggc
     3901 gggccacgag ctcgtcgaga cggcggagga ggacgattac acgaggttcg tcgtccgcaa
     3961 gacgaggtga ccccagcaga tgcgcgcgaa catcgtgaag cccgttcagt ctgagttcgc
     4021 ctggagtcga tcgccgtatg tgggctgacc gttccggcgg cccaaggagg ggggagaaat
     4081 ggatcagaga cttggcagcc cgcggtgggc ggcgagatac gggcgtatcg ccctcgtctt
     4141 cgtggcgctc gccaccctgg ccgcatgcaa cggcgacgga ggcggcacgg tggatgtatc
     4201 gctccaggag ttctcgatcg ctgctgatcc cgcgacggtg gatgccggcg aggtggtctt
     4261 cgaggccacg aacgacggac cgaacgatgt ccacgagttc gtggtcttcc gaaccgacct
     4321 cgacccgacc gcgcttccga ccgacgagaa cggtgccgtc gtcgagacgg gcgagggcat
     4381 agagctgatc ggcgagatcg aggacatccc ggtcggggag acccagagcg tgaccttgga
     4441 cctggaggcc ggcaactacg tcctgatctg caacatcttt gacgaggacg agcaggaggc
     4501 ccactaccag gaagggatgc gcacgacctt caccgttacc tgagagagca gactggaacg
     4561 cttcggcgtc ggtcgtcttg acgaccgggc cgttgtcgct gcgtcccgcg tctgtcgaac
     4621 cccgctgagg acacgcaggt caagtgtgct tcggccgtga cggccaccgc tccggatcgc
     4681 atctttggtc acccccgact ctcatccggg gccgtgtgcc ccctgcttgc gctgcggcat
     4741 tccgcccctc ctacggcagg gcgcttggcc ctcttggacg gatcacgtgc ttacccccgg
     4801 ccgcgtccag aacgaaggca tcgcccatca cgtcgcgcac aggctgacgg gtgatgagcg
     4861 cgctcacgat gggccgggtg cccggttgtc catcggcagt gaagtccatg gtggtcccat
     4921 ctcgcgtcct gcgacgacga gaccggcgtc ccagccgggc gctcgaccgc cgggtggcgg
     4981 ctggatctgc tcggtctcca cgccagcacg tgcgagcagc cacgagccgt agaccttccc
     5041 gttccaacgg accacgtggc cgcccgcgcc caaagggatc cagtacaggt cgactgatcc
     5101 gggcgccggc gcggacgtca tcagccctgc gtgccagggg tggtcgtgtg gcgggcaagg
     5161 taggcggcgg cctcggcccg ccgcgcgacc tcgagcttgg acaggatcct cg
//
LOCUS       DASUYD010000083         2435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_705170, whole genome shotgun sequence.
ACCESSION   DASUYD010000083 DASUYD010000000
VERSION     DASUYD010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705170"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VF195_05020"
     CDS             <1..851
                     /locus_tag="VF195_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580214.1"
                     /translation="PSSIAEFVRVIGPLTDPRGHGGDPTDAFHVVAPSLPGFGFSSPL
                     EDSGWESTRTAKAWVELMHRLGYERYGVHGGDIGAGISGDLGIHDPDRVVGAHVSTDP
                     TALALIGGMLPDETENMTEAQKGRLEELRGWEADGRGYLQIQTTRPRTLAYGLNDSPA
                     AQLAWIVEKFKEWTNPAAELPEDAVDRDQLLTDVSIYWFTGTGASAARFIYEAAHADR
                     DWGAMSPAPTGMAVFAADNLLGYVLNGDGHVEHWSEFESGGHFPAMEEPELLVGDIRK
                     FFRDLR"
     gene            992..1873
                     /locus_tag="VF195_05025"
     CDS             992..1873
                     /locus_tag="VF195_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019068970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580215.1"
                     /translation="MASTKDATTTVTDHEAKQVERANEAGRVPVVFVHGLWLLPTSWD
                     RWAKLFEKNGYTALTPGWPDDPDTVAEAKTHPEVFANKSVGEVADHFEDVIVGLKRKP
                     AIIGHSFGGLLTQILAGRGRAAVSVAIDPAPFRGVLPLPISSLRAASPVLRNPLNVNR
                     AVPLTYEQFRYSFANAVSEDEAKQLYETFAVPTPGEPIFQAAAANLNPWTDAKVDTTN
                     PKRGPLLIIDGERDHTVPWAIAKASYKRQARNKGVTEIVKIANRGHALTIDNGWREVA
                     DTALAFVRKHGPKVEAA"
BASE COUNT          422 a          801 c          840 g          372 t
ORIGIN      
        1 atccgagctc gatcgccgag ttcgtgcgtg tgatcggccc gctgaccgat ccgcgagggc
       61 acggcggcga cccgaccgat gcgttccacg tggtggctcc gtcgctgccc gggttcgggt
      121 tctccagccc tctcgaggac agcggatggg agtccacccg cacggccaag gcctgggtgg
      181 agctgatgca ccgcctgggc tacgagcgct acggcgtcca cggaggcgat atcggagcgg
      241 gcatctctgg cgacctcggg atccacgacc ccgatcgcgt cgtcggcgcg cacgtcagca
      301 ccgatccgac cgccctggcc ttgatcgggg ggatgctgcc cgacgagacc gagaacatga
      361 ccgaagccca gaagggccgg ctcgaggagc tgcgaggctg ggaggccgac ggccggggct
      421 acctccagat ccagacgacc cgaccgcgga cgctggcgta cgggctcaac gattcgcccg
      481 cggcgcagct cgcgtggatc gtggagaagt tcaaggagtg gaccaacccg gccgccgagc
      541 ttcccgagga cgccgtcgat cgggaccagc tgctcaccga cgtcagcatc tactggttca
      601 cgggcacggg agcgtcggcc gcacgcttca tctacgaggc tgcccacgcc gatcgcgatt
      661 ggggagcgat gtctccggcg ccgaccggga tggccgtatt cgccgccgac aacctcctcg
      721 gatatgtcct gaacggcgac gggcacgtcg agcactggtc ggagttcgag agcggcggcc
      781 atttcccggc catggaggaa cccgagctgc tcgtcggcga tatcaggaag ttcttccgcg
      841 atcttcgctg accccatgtg tccggcgcga gctgtcggct ccgcgccggc cccgcgtcgg
      901 cctgaccgat gggtcgctaa ggcgagcccg acgtcgatgg tcggggttgt gatatccctg
      961 tcgccatcga ggcggaccga ggggagtacg catggcatct acgaaggacg cgacgacgac
     1021 ggtcaccgac cacgaggcca agcaggtcga gcgcgcgaac gaagcagggc gcgtacccgt
     1081 cgtgttcgtc cacggcctct ggctgctgcc caccagctgg gatcggtggg cgaagctgtt
     1141 cgagaagaac gggtacacag cgttgacacc cggctggccg gacgaccctg acacggtcgc
     1201 ggaggccaag acacaccccg aggtcttcgc gaacaagtcg gtgggcgagg tggcggacca
     1261 cttcgaggac gtcatcgtcg ggctgaagag gaaacccgcc atcatcggcc actcgttcgg
     1321 gggcctgctc acgcagatcc tcgcgggtcg tgggcgggcg gccgtgtcgg tcgcgatcga
     1381 cccggctccc ttccgagggg tgctgccgct gccgatctcg tcgttgaggg cggcgtcgcc
     1441 ggtcctccgc aatccgttga acgtcaaccg ggcggtgccg ctcacctacg agcagttccg
     1501 gtactcgttc gccaacgccg tgagcgagga cgaggccaag cagctctacg agaccttcgc
     1561 ggtcccgacg ccgggcgaac cgatcttcca ggcggcggcg gccaacctca acccgtggac
     1621 cgacgcgaag gtcgacacca cgaaccccaa gcggggcccg ctcctcatca tcgacggcga
     1681 gagggaccac acggtcccgt gggccatcgc gaaggcttcc tacaagcgcc aggcgcgcaa
     1741 caagggcgtg accgagatcg tcaagatcgc gaaccgcggc catgcgctga cgatcgacaa
     1801 cggctggcgt gaggtcgccg acacggcgct cgcgttcgtc cgcaagcacg gtccgaaggt
     1861 cgaggcggcc tgagtcgtcg ggtgtacgga tcccaatagc ctggctcggg atcgatctgc
     1921 tgcaccacgt ccacccagac gccggccggg tactgagtcc gaatcgcctc tgaccccacg
     1981 gttcgtctcg tagcgagtgg tcgacgacga gttcggacgt cgcggtcgaa ctcggacgcc
     2041 gcgtcgagac ctgaagggta aggaggatgc cttcgcttgt cgaaggcttc gggacccggt
     2101 ggccgcgacc ggtggtccgg ggtcatgaag gcgatcccgt gttggccctc ctccgacgag
     2161 gtcagataac gaaccacgtg gcctcgaaga cgactcggaa tccgaaccag cgggtgtaga
     2221 agtcgcggca ctcgatcagc ttgtcggtca cgacgaccgg gtatgaggcc tcgagccatg
     2281 gaggactcct tccgattgaa ctcggtcagt tccgaggcga ccattccgag ctgacctcga
     2341 cgggagatct ttggaacatg agcgagcgga cgaccgagga acgcgagcgc gttctccgca
     2401 ccttggcgag gggtgggttc gtcgcccccg gtgcg
//
LOCUS       DASUYD010000084         2678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_705566, whole genome shotgun sequence.
ACCESSION   DASUYD010000084 DASUYD010000000
VERSION     DASUYD010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2678
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705566"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2678)
                     /locus_tag="VF195_05030"
     CDS             complement(<1..>2678)
                     /locus_tag="VF195_05030"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX6580216.1"
                     /translation="SERSPEELFGVISDAFELADARRADEFAVRVFEPSPPENGDEAP
                     GTVVEISAPDSPFLYDSVAEELDAWGLDARRVIHPVIGVERGSEGRIVRVLHVRQTED
                     RESLMHFELEQRLTEQERTELERAIRGILGDVRLAVRDFEPMKEAARGMIDIASAGSA
                     LYPADEVAETVAFLEWLLDLNFIFLGYREYELVDLPKGRALAAVPGSGLGILSKVDWS
                     AYEQPVPLETIEPNLRARIEGGDLLIYSKTNRPSTVHRRARMDYIGVRRVSPDGRIIG
                     EARMVGLFTSKAYAEPASKTPLLHRKLEQIVDAEDLFEGSHDYKAVVSIFESFPKDEL
                     FAAPTEELRQQVMGLLQLQESRHTRLFVRRDLYGRSVSLLVAIPRDRFNAEVRQRLQE
                     LFLERFNGTTIDVHVSLTESDLAQLHFTVHIGQGQIPDVSFDELQRDVVEIARTWDDQ
                     LLDRLVEEHGQARARELFERWADRLPDYYKASTDVAIAGLDILRFDELEHGEEAFLIG
                     LANETEEGQSLTHVRLYKADGKVELSDFVPTLESLGLRVIEEWPTTLSGEEGDERFLH
                     DFGVLGADHRPIDVDATGDRVAECIAAVWRGECESDSLNRLVVSAGVDWRQVQILRAY
                     RKYHHRVNSSFPVEYKNDAFAAHPAIAAQLVELFEKRFDPSRPRDPQAVEALGERIRS
                     DLDAVRSLEVDRILRNALGVVDATVRTNAYVPRRRAIAFKFRSAAVPEMPKPTPLFEI
                     FVYSPEMEAIHLRGGRVARGGIRWSERRQDYRTEILGLLKAQMVKNAVIVPTGAKGGF
                     VLKHEVTDPRASTDEGTRQYVTFVRSMLEVTDNLVDGVVVHPDHVVVHDEDDPYLVVA
                     AAKGTATFSDTANAISAEYGFWLGDAFASGGSTGYD"
BASE COUNT          430 a          914 c          884 g          450 t
ORIGIN      
        1 ggtcgtagcc ggtggagcca ccggaggcga acgcgtcacc tagccagaac ccgtactccg
       61 cggagatcgc gttcgcggtg tccgagaagg tggcggtgcc cttggcggcg gcgacgacga
      121 ggtaggggtc gtcctcgtcg tgcaccacga cgtgatcagg atgcacgacc acgccgtcga
      181 ccaggttgtc cgtcacctcg agcatgcttc gcacgaaggt cacgtactgt cgcgtgccct
      241 cgtctgtcga ggcccgtgga tcggtgacct cgtgcttgag gacgaagccg cccttggcgc
      301 cggtcggtac gatgacggcg ttcttcacca tctgcgcttt caggagaccg aggatctccg
      361 ttcggtagtc ctgtcgccgc tcggaccagc ggatcccgcc gcgagccacc ctcccgcccc
      421 gcaggtggat cgcctccatc tcgggcgagt acacgaagat ctcgaacagg ggcgtcggct
      481 tgggcatctc gggaacggcc gcggagcgga acttgaacgc gatcgcgcgt cgcctcggga
      541 cgtaggcgtt ggtgcgcacc gtcgcgtcga cgacgcccaa ggcgttacgg aggatccggt
      601 cgacctcgag cgatcgtacg gcgtccagat ccgagcggat cctctctccg agcgcctcga
      661 ccgcctgtgg gtccctcggc cgggacggat cgaaccgctt ctcgaacagc tccacgagtt
      721 gggcggcgat ggccgggtgc gccgcgaagg catcgttctt gtactcgacc gggaagctcg
      781 agttcaccct gtggtgatac ttgcggtagg cacgcaggat ctgaacctgt ctccagtcga
      841 cgcccgcgct gacgacgagc cggttcagcg aatcagactc gcactcgcct cgccacaccg
      901 ctgcgatgca ctcggcgacc cggtcaccgg tggcgtcgac gtcgatcggc cggtgatcgg
      961 ctcccagcac gccgaagtcg tggaggaagc gctcgtcgcc ctcctctccg gagagggtag
     1021 tcggccactc ctcgatcacg cgaagaccga gcgactcgag cgtcggcacg aagtccgaga
     1081 gctcgacctt gccatcggcc ttgtagagcc ggacgtgcgt gagcgactgc ccctcctcgg
     1141 tctcgttcgc cagaccgatc aggaaggctt cctcgccatg ctcgagctcg tcgaagcgga
     1201 ggatgtcgag gccggcgatg gccacgtcgg tcgacgcctt gtagtagtcg gggaggcggt
     1261 ccgcccagcg ttcgaacagc tctcgcgcgc gtgcctgccc gtgctcctcg accaaccggt
     1321 ccagtagctg gtcgtcccac gtgcgggcga tctcgacgac gtcgcgctga agctcgtcga
     1381 aggacacgtc cgggatctgg ccctgaccga tgtgcacggt gaaatgcagc tgcgccaggt
     1441 cgctctcggt gagcgagacg tggacgtcga tcgtcgtgcc gttgaagcgc tcgaggaaca
     1501 gctcctgcag ccgctggcgc acctccgcgt tgaagcggtc gcgcgggatc gcgaccaaca
     1561 gcgagacgct ccgtccgtag aggtctcgtc gaacgaacag acgggtgtgt cgggactcct
     1621 gcagctgcag cagccccatc acctgctgtc ggagctcctc ggtcggagcg gcgaacagct
     1681 cgtccttcgg gaagctctcg aagatcgaca ccaccgcctt gtagtcgtgc gacccctcga
     1741 acagatcttc ggcgtccacg atctgctcga gcttgcggtg cagcagcggc gtcttgctcg
     1801 cgggctccgc gtacgccttg gaggtgaaca agcccaccat ccgcgcctcg ccgataatgc
     1861 gtccgtcggg cgagacccga cgcacgccga tgtagtccat ccgggcgcgc cgatgcaccg
     1921 tcgagggcct gttcgtcttc gagtagatga gaagatcgcc gccctcgatg cgggcgcgca
     1981 ggttcggctc gatcgtctcg agcggcaccg gctgctcgta cgccgaccaa tcgaccttcg
     2041 acaagatgcc gaggcccgat ccggggaccg cggcgagggc ccgccctttg gggagatcga
     2101 cgagctcgta ctcgcggtag ccaaggaaga tgaagttcag atcgagcagc cactcgagga
     2161 acgcgacggt ctcggcgacc tcgtccgccg ggtacagggc cgacccggcg ctcgcgatgt
     2221 cgatcatgcc ccgtgccgct tccttcatcg gctcgaagtc ccgaacggcc aggcggacgt
     2281 ctccgaggat gcctcggatc gcccgctcga gctcggtccg ctcctgctcg gtcagccgct
     2341 gctcgagctc gaagtgcatc aaggactctc ggtcctcggt ctgtcggacg tgcaggaccc
     2401 ggacgatccg gccctcggaa cctcgctcga cgccgatcac ggggtggatc acgcgacggg
     2461 cgtccagccc ccaggcatcg agctcctcgg ccaccgagtc gtacaggaac ggggaatccg
     2521 gggcgctgat ctcgaccacc gtgcccggcg cttcgtcgcc gttctccggc ggactcggtt
     2581 cgaacactcg caccgcgaac tcgtccgccc gtcgagcgtc tgcgagctcg aaggcgtccg
     2641 agatcacgcc gaagagctcc tcgggcgacc gctcggag
//
LOCUS       DASUYD010000085        17879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1148103, whole genome shotgun sequence.
ACCESSION   DASUYD010000085 DASUYD010000000
VERSION     DASUYD010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17879
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148103"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VF195_05035"
     CDS             complement(<1..856)
                     /locus_tag="VF195_05035"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEX6580217.1"
                     /translation="MTRTFTTAATIEDALEALGSGARPIAGGTDLVVGARQGKSPLPE
                     AIVAIDRIPALEGIVGGDDGLRLGALVTHEEIVANDIVRARFPALADACAIVGSHATR
                     AHGTIGGNVMNASPAMDTGGPLLCFDASARLRSASGEREAPLDELWTGPGTTAVDPRE
                     LLVGIDVPVQIPATGSAYVRLEYRRQMEIAVVGATAVVTLDASTVADARVAITALAPT
                     IRRVPEAESALVGSEGDDDAVALAAAAAAAAAAPISDVRASERYRRAMADVIARRAVR
                     AALERARGH"
     gene            complement(853..3126)
                     /locus_tag="VF195_05040"
     CDS             complement(853..3126)
                     /locus_tag="VF195_05040"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX6580218.1"
                     /translation="MAVGISAAKTFIRPDGREKVTGAGRYTADLVSTGMAHAAFRYAD
                     HPHAKILRIDTTKALALSGVVAVVTQDDLPDVRFGGLVQDRTLFAKDVVRFEGEIVAG
                     VAALTETAAAEAARLIEVEYEPLPALTDFVAAMDADAPLVHADWASYERDEQMVADGN
                     TISYATIVKGDADAAMARADVVVKSRYESDASQGVPIEPRAVLAEWRGDQVTIWSSTQ
                     VPYAARSGVAQTLQIAEANVRVVVPLLGGGFGAKCDLHFEAQVAALARAARRPVRLVF
                     SREEEFRAIAQRREGIVMEFETGAMRDGRLVARRARLVLDKGAYCGEGGFLGQMAAMH
                     ACGPYVIDAISVESFLNYSNRQPSGSVRAPTAPQVCWGLEQHMDEVAGELGIDPVELR
                     RRTLIEEGAEGPTRQVFDEIGVKQTLERAVEMIGYERDLPEDEAIGVAVGWWPSGPAP
                     SGAFIHLNGDGSGTIVTGAQENGSGAVMAMPGFVAEDLAMEPENFTLLYQDTAAANWD
                     MGSCGSQTTFNSGRAILAAAADVRGQLLDAAAAELEVDPEDLELAEGQVRVKGSPDRS
                     VSIADIAGAGTIHGKGSGDVPEVPAGDTGGCVGRLGSETFAAPQLITQAAHVKVDRGT
                     GVVRVLKVAAAHDSGIILNRMGADGQVTGGVVMGVGLALSEGTVFDDDGRQRNPHLLD
                     YKLVTCSDAPEIEIDWVQIPTENAGPRGSKGVGEPPQVPTAAAIANAVAKVLGRRVRQ
                     LPMTPERVWAASAEVGS"
     gene            3272..5197
                     /locus_tag="VF195_05045"
     CDS             3272..5197
                     /locus_tag="VF195_05045"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase UvrD2"
                     /protein_id="HEX6580219.1"
                     /translation="MPDLESVLAGLNDSQREAALALRGPVAILAGAGTGKTTTITHRI
                     ACQVRSGAFEASQVLAVTFTDKAAGELKRRLRALEVDGVEARTFHAAALSQLARLWSA
                     HTGGPLPGVLDAKAPLIASLANALPPPHRFLPRRELASEIEWAKNRMIEPARYLAELG
                     DHEPPIPAELMLRIYDGYERRKRAMGRLDFEDMLTLALRLFDDHPDAAAAVRARFSAF
                     TVDEFQDVNLLQAGLLDRWLGDRDDLCVVGDDYQTIYAFTGASPDHLLDFTRRFPRAT
                     VVRLEENYRSSPEILELANRLAPRLGGIRKTLRPNRPSGPSPVARAEPDEGAEVTAVV
                     DAVRRLHLEEAVPLEEIAVLSRINARSEPFEEAFAAAGIPYQVRDGAFLRRPGPRGVV
                     RRLERASADAAVAEAVVGITAQLGYDPEASPDADEEVTRQSDLARMRSLAAEFQGTHP
                     AGGPAAFLAELAERFSTAHSSRGVNLLTYHRAKGLEFDAVFLPRMIDGELPFRSGRAK
                     ADPREERRLLYVGITRARRYLFLTWPRSGRSRPSPFLDEMELSPAGLPARTSSPGAPV
                     AATRGGPLFDRLKDWRRKRAQADGVPAYVVFHDRTLAEIAERKCKDWADLAAVSGVGP
                     AKLERYADEILAVVAGS"
     gene            complement(5339..6523)
                     /locus_tag="VF195_05050"
     CDS             complement(5339..6523)
                     /locus_tag="VF195_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6580220.1"
                     /translation="MSDVATNERYDVEALRLERLARLQAAMHAHDVEVCLFFNEPNIR
                     YATGASAMPVYAMSTFVRCALVPAEGTPILFEHGNSMHRSALRAPDVRRMHAWEFFDD
                     PEKEADAWADELLEAVREVGAEGSTVAVDRLGAPAYLALARRGIGVGDSAPVTQDARR
                     VKTPQELRLLDENAALVMEMLAALDRAIAPGVSERDLLAVLAGVMIRGGGEYLATNTV
                     CSGPNTNPWRAEVTDRRLESGDLVYVDTDTIGIEGMFFCVSRTFLAGDTPPSTAQRDA
                     YRASHEWLRGMEELIRPGVTCGDLATLAPPMPERYLAQRYECMVHGVGLEEENPSVCH
                     PQDRQSNADTVIEEHMALVVELYAGEPGGRDGVKLGDQVVVTSDGCRVLCPYPFSTTL
                     LA"
     gene            6572..7840
                     /locus_tag="VF195_05055"
     CDS             6572..7840
                     /locus_tag="VF195_05055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEX6580221.1"
                     /translation="MRVPVEAPWSRWWTVDALLYGCSALFAGLTAGAAGIPLQRSWGR
                     VALWPYALAAAIALVAARISGSEQQQRRRRTFLVAVVFVAATIAPMAVAASRRAGGDP
                     GAYAQSEVIIVEEAAIALLEARDPYVAEYRDGPLAERPVATQVHFPYLPGMLAFGIPR
                     ALADPATWTDARVWFTVVALAVAGMALLRRAVAPEDRLRAFQMLFALPTGALLLATGG
                     VDIPVIAMLLGAAVLARNDRPVAAGVMGGLALATKQTSILVLPFLVLAIPRGRPRRRF
                     VITVGAVGAALIVPFALWDVDAFAEDTILFPLGLGQGESAAATPTLGSLLVDLFPSSR
                     TAVTVLLVTAIVIIVASLLFRDRGPSLSQACARSGAAFVAAIALAPAARVGYLVYPVN
                     LIVWAIAFRPPSAGGRGRFRRQVLREPSAG"
     gene            complement(8052..9320)
                     /locus_tag="VF195_05060"
     CDS             complement(8052..9320)
                     /locus_tag="VF195_05060"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX6580222.1"
                     /translation="MTDDAHDHAVSAERLFDAVQGHLEGAAERLRLEDGARKVLSTHR
                     REVAVSVRVPRDDGSFDVYPGWRIQHNGARGPFKGGLRFHPNASRDEFRCFSALMTWK
                     TALLDVPFGGAKGGVQVDPKSLSVAELERLCRSYLSSIELVIGPYRDIPAPDVNTGPR
                     EMAWMYDEYSRSRGDWPAVITGKPVKLGGSLGREAATGRGALFALDRIARSRRWTREH
                     IRIAVQGFGNAGAWFAELAHQLGYRVVAVSDSRGAVVNGEGLFPRDVLRHKQQTGSVT
                     GFKHGDTIEGDDLIGTECEVLAPAALEESLRVDNAERVRANLVLEIANHPTTPDADRL
                     LHDRGVWVIPDILGSAGGVTVSYLEWGQNLQRERWTEERVNGRLQELMESATDLVLER
                     ADEGGVSHRAAAYEIAVERVADAGRARGWH"
     gene            9569..10573
                     /locus_tag="VF195_05065"
     CDS             9569..10573
                     /locus_tag="VF195_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HEX6580223.1"
                     /translation="MLSFGVTFMLDPPVSRTIEWAKLAEAQGFDAVWAWDSHVLWQEF
                     YTTFALVAANTERVRLGPCVTNPATRDMTVTASAMATLQEVSGGRMDIGMGRGDSARR
                     VIGQKPVTVEHMEHACRFIKDLAEGREVDHEGTPLQLRWSEGHRLPVWVAAYGPKALR
                     ACGRVADGLIMQLADPFILGWSLAYVREGAEEAGRSFEDIQIQVAAPGYLAEDLAMAR
                     DQVRWFPALVSNHVVDLVKRYSDADLPQALTDYIEARDHYDYADHGRTGAEHADFVTD
                     EVVDRFCVLGTADACIGKLRELEALGVTQFNIYSMQEDPGPAGIIEGFGKDVLPAFRT
                     "
     gene            10614..11459
                     /locus_tag="VF195_05070"
     CDS             10614..11459
                     /locus_tag="VF195_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020373615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX6580224.1"
                     /translation="MTMVRAAVIQAHANMPKDDAVDKHVELIAKAAADGAEVTCLQEI
                     FHGPYFCAEQDPKWYATAEPEDGPLVTRMREVAKTHNMVLVVPWFEEAQTGVYYNSAV
                     VIERDGTIAGKYRKTHIPHVGPCFWEKFYFKPGNLGYPVFDTSVGKVGLIICYDRHFP
                     EIGRALGLKGAELVFNPSATVESLSKYLWELEQPAHAVANGYWIAAINRVGTEEPLSS
                     AKFYGSSYFCDPRGQIVAQASDSEDEVLVHDVDLDMIRDVRNTWQFLRDRRPELYGEL
                     TQLLP"
     gene            11513..12889
                     /gene="hydA"
                     /locus_tag="VF195_05075"
     CDS             11513..12889
                     /gene="hydA"
                     /locus_tag="VF195_05075"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX6580225.1"
                     /translation="MVRTLIQGGTVVTAMETVAADVLVDGEKVAAIGTGMSVEPDRTI
                     DATGRYVMPGAVDVHTHMDLPFGGSFCSDDFATGSRAAAFGGTTTIVDFALQDYGESL
                     RTGLDRWHEKAQKSSIDYGFHMIVREVNDQVLQEMDTLVGEGVTSFKLFMAYPGVFML
                     DDGAIFQAMQRAADSGALIMMHAENGGPIDVLIRQSLGQGRTDPVNHSLTRPPLMEGE
                     AVHRVFKMAELAGAPAYIVHLSSRDALNEVREARDRGVAAYAETCPQYLYLSIDDMAR
                     PGFEGAKFVCSPPLRTADHQEELWKGLLADDLQVVSTDHAPFNFKGQKDLGKDDFSKI
                     PNGLPSVEDRFTLLYQGVVDGRIGLNRFVEIVATAPAKMFGLSARKGTIAPGFDADIV
                     IFDPDHERTISAETHHMNVDYSCYEGRRVKGLPQIVLQRGSVLVDNGEFHAKEGQGSF
                     LPRSRIST"
     gene            12886..13302
                     /locus_tag="VF195_05080"
     CDS             12886..13302
                     /locus_tag="VF195_05080"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580226.1"
                     /translation="MTIDVERLRRLPLFGELDHHDLAMIAHHVGEMEASEGQVLFEQG
                     SIPYEMFVIEEGTADVTRDGQTVGRIGPGDVVGEIALLRFRRRMGTVRVTSPLRAVTV
                     SADELQVIEEEMPEIAGNLRSIMTAREREIADRADD"
     gene            14194..15036
                     /locus_tag="VF195_05085"
     CDS             14194..15036
                     /locus_tag="VF195_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580227.1"
                     /translation="MKTPRFWRVLGSGALALLLAGCFKVNMDLDVTADNTVSGTAVVA
                     VEESVLELSGQNVDQLFADMDLSDLPPGSKADPYEEDGFVGRQITFEDVPLDAFTGNG
                     TLSGTASGEELDIVREGDEFLVTGGFDMSGQEFTGTEIPQQFLENFEFRISITFPGPV
                     SSASGDIDGNTVTWEPRVGQNTEIQAVASAIPSSSSPLLMIVLIAAGALVLGAIVYFL
                     THRGAAAADPIAGTGFGDAVPAPVPARPEASPIDVPPTAFPAPDDASPVDDTPPPPPP
                     SSGS"
     gene            15042..15263
                     /locus_tag="VF195_05090"
     CDS             15042..15263
                     /locus_tag="VF195_05090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX6580228.1"
                     /translation="MPTYEYRCGSCGATFESRRTIAHADAPILCPHGHGNAVRRPSVF
                     AVTSSVTAERAARPAGTGCGPDCARAVNG"
     gene            15372..15815
                     /locus_tag="VF195_05095"
     CDS             15372..15815
                     /locus_tag="VF195_05095"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEX6580229.1"
                     /translation="MTYRRLSLLVLVVAVLIAGMPSPAGADNITARRKMLHLLNQTRG
                     NHGLSALRLNANVSHFAWKHSKKMAGRNRCFHTANLYDVVRTYSPSTWGENVGAAGTL
                     LRIRTLWMGSGGHRANILNPRFRRIGIGVVKARGLLWVTTIFYGG"
     gene            complement(15821..>17879)
                     /locus_tag="VF195_05100"
     CDS             complement(15821..>17879)
                     /locus_tag="VF195_05100"
                     /inference="COORDINATES: protein motif:HMM:NF044969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX6580230.1"
                     /translation="GVSERVGYWLGDAFASGGSHGYDHKELGITARGVWESVKRHFRE
                     LGTDPMTEPFTVVGIGDMSGDVFGNGMLYTDRIRLVAAFDHRHVFLDPQPDPAISFAE
                     RKRLYGTPGSSWGDYDRSVLSEGAEVIDRTAKWVTLSAQAREALAIPDDTPAEMTPAD
                     VIRWVLQAPVDLLWNGGIGTYVKAANEGHTEVGDRANDPVRINGSQVRARVVGEGGNL
                     GFTQRGRIEYALTGGRINTDFIDNSAGVDTSDREVNLKILLGLAIERGELTTDERNAL
                     MEASTDDVVRRVLYDNYLQAQILSQEVAVAAERNETCEDLMLQLEAEGELDRLVEFLP
                     TSDEMAERRGRGEGLTRPEIALLVAYSKRSIKRDLLEADLPDAEYLEQDLARYFPETI
                     VDRFRPMLTEHPLRRELIATIVSNDVVNSQGVTFVSRLVAETGATPAEVVRAFRIARD
                     VVGAVPRWDAVESLDGKIDPEIQNDLMIGVDRLVESTARWYLVRAPGLRLGEAVGQAR
                     TAFRELSSVIEQIGPEAWREQREREATRLTAEGIPDELARRHAFQDELVHGPDIISVS
                     RATGRPVLEVARGFFLLGERLDIDWLEQRLAEQPAKTRWQRWAVHSMEDDLYTIRREI
                     IETVLEHAGGRPMDEAVDAFLEDRSAAYGRVQRFMRSLAMEGVTDLSQLTVALRQLRS
                     LVS"
BASE COUNT         2751 a         6296 c         6068 g         2764 t
ORIGIN      
        1 tgtgtccgcg agcccgctcg agcgcggcgc ggacggcgcg tcgcgcgatg acgtcggcca
       61 tcgcccgccg gtagcgctcc gaggcacgga cgtccgagat cggtgccgcg gccgcggccg
      121 cggccgcagc tgccagggcg accgcgtcgt cgtccccttc ggatccgacg agtgccgact
      181 cggcctcggg cacgcgtcgg atcgtcgggg cgagagccgt gatcgccacc ctggcgtcag
      241 cgacggtcga ggcgtcgagc gtgaccacgg cggtcgcgcc gaccacggcg atctccatct
      301 ggcggcggta ctcgaggcgg acgtacgcgg agccggtcgc cggaatctgc accggaacgt
      361 cgatccccac gagcaattcg cgcggatcga ccgcggtcgt tccgggtccg gtccagagct
      421 cgtcgagcgg cgcctcccgc tcccccgacg ccgaccggag acgggcggat gcgtcgaagc
      481 acaggagcgg cccgccggtg tccatggcgg gcgaggcgtt catcacgttg cccccgatcg
      541 tgccgtgcgc tcgggtagcg tgggagccga cgatcgcgca cgcgtcggcg agggccggga
      601 accgcgcccg gacgatgtcg ttcgcgacga tctcctcatg cgtcacgagc gcgccgagac
      661 gcagtccgtc gtcacccccg acgatcccct cgagcgcggg gatccgatcg atcgcgacga
      721 tcgcctccgg gagcggagac ttcccctgcc gggccccgac cacgagatcg gtgcccccgg
      781 cgatcggccg cgctcccgag ccgagggcct cgagcgcgtc ctcgatcgtc gctgcagtcg
      841 tgaacgtcct cgtcacgacc cgacctcggc ggacgccgcc cagacccgtt cgggcgtcat
      901 cgggagctgc cggacacgcc gcccgagcac cttggcgacc gcgttcgcga tcgcggcggc
      961 ggtcgggacc tgcgggggct caccgacgcc cttggagccg cgcgggcccg cgttctccgt
     1021 cgggatctgc acccagtcga tctcgatctc aggggcgtcg gagcaggtga cgagcttgta
     1081 gtcgagcaga tgcgggttcc gctgccggcc gtcgtcgtcg aacactgtcc cctccgagag
     1141 cgcgaggccg acccccatca cgacgccgcc cgtcacctgg ccgtcggcgc ccatcctgtt
     1201 gaggatgatg ccagagtcgt gggcggcggc caccttcagg acgcggacca caccggtccc
     1261 gcgatcgacc ttcacgtgcg ccgcttgggt gatcagctgc ggggcggcga acgtctcgct
     1321 cccgagacgg cccacgcatc cgcccgtgtc gcccgcgggg acctcgggca cgtcgcccga
     1381 gcccttcccg tggatcgtgc cggccccggc gatgtccgcg atcgacacgg accgatcggg
     1441 cgagcccttc acccgcacct gcccctcggc gagctcgagg tcctccgggt cgacctcgag
     1501 ctcggcggcg gcggcgtcga ggagctggcc gcgcacgtcc gcggccgcgg ccaggatcgc
     1561 gcgaccggag ttgaaggtgg tctgcgaccc gcacgagccc atgtcccagt tcgccgccgc
     1621 ggtgtcttgg tagagcaggg tgaagttctc cggctccatg gcgagatcct cggcgacgaa
     1681 ccccggcatc gccatgacgg ctccgctgcc gttctcctgg gcaccggtca cgatcgtccc
     1741 gctgccgtcg ccgttcaggt ggatgaaggc gccggacgga gcgggcccgg agggccacca
     1801 gcccacggcg acgccgatcg cctcatcctc ggggaggtcg cgctcgtacc cgatcatctc
     1861 gacggcgcgc tccaaggtct gcttcacgcc gatctcgtcg aagacctgac gcgtcggacc
     1921 ctccgcgccc tcctcgatca acgtgcgccg gcggagctcc accggatcga taccgagctc
     1981 cccagcgacc tcgtccatgt gctgctccag gccccagcac acctggggag cggtgggcgc
     2041 ccgcaccgag ccggacggct gccggttcga gtagttgagg aacgactcca cggagatggc
     2101 gtcgatcacg tatgggccgc acgcgtgcat cgccgccatc tgcccgagga atccgccctc
     2161 gccgcaatac gcgcccttgt ccagaacgag ccgagccctc cgcgccacga ggcgtccgtc
     2221 ccgcatcgcc cccgtctcga actccatcac gatcccctcg cggcgctgag cgatcgcccg
     2281 gaactcctcc tcgcgggaga agaccagtcg aaccggacgg cgcgcggcgc gcgcgagggc
     2341 ggccacctgc gcctcgaaat gcaggtcgca cttcgctccg aacccaccgc ccaggagcgg
     2401 gacgaccacc cgaacgttcg cctccgcgat ctgcagggtc tgcgcgacgc ccgagcgtgc
     2461 ggcgtagggg acctgggtcg aggaccagat cgtgacctgg tcgccgcgcc actcggcgag
     2521 gacggcgcgc ggctcgatcg ggacgccctg cgatgcgtcg gactcgtagc gactcttcac
     2581 cacgacgtcg gctcgggcca tcgcggcgtc cgcgtcgccc ttgacgatcg tcgcatagga
     2641 gatcgtgttc ccgtcggcca ccatctgctc atcgcgctcg tacgaagccc agtcggcgtg
     2701 gacgagcgga gcgtccgcat ccatcgccgc gacgaagtcc gtcagcgccg gcagcggttc
     2761 gtactccacc tcgatcaggc gcgccgcctc ggccgcggcg gtctcggtga gcgcggccac
     2821 ccctgccacg atctctccct cgaaccgcac gacgtccttc gcgaacaggg tgcggtcctg
     2881 cacgaggccc ccgaagcgga cgtcgggcag gtcgtcctgc gtgaccaccg cgacgacgcc
     2941 ggacagcgcg agggccttcg tcgtgtcgat gcggaggatc ttcgcgtggg gatggtccgc
     3001 gtagcggaac gccgcatgcg ccatgccggt ggacacgagg tcggctgtgt agcgccccgc
     3061 gcccgtcacc ttctcccgac cgtcggggcg gatgaacgtc ttcgctgccg agatgccgac
     3121 cgccatcaga tgccctccgt tccgatggcg cacatcatgg tcggtgtccg cagctccgac
     3181 aaacgcccgc ggacgcgact ccgatcatgc ggccgcggaa gtgatctcgg tcacggccgg
     3241 gcgccaccgg tccgatgcta gggtcgcccg ggtgcccgat ctcgagagcg tgctcgccgg
     3301 actgaacgac tcccagcggg aagccgcgct cgcgctccga ggacccgtgg cgatcctggc
     3361 cggcgcgggc acggggaaga cgaccacgat cacgcatcgg atcgcctgtc aggtccggag
     3421 cggagcgttc gaagcctcgc aggtcctcgc tgtgaccttc accgacaagg cggcgggtga
     3481 gctgaaacga cggctccggg cgctcgaggt ggacggggtg gaggcgcgca cgttccacgc
     3541 cgccgcgctg tcacagctcg cgcgactgtg gtccgcacac accggcggtc cgctccccgg
     3601 tgtcctggat gccaaggctc cgctcatcgc ctcgctcgcg aacgcgttgc ccccgcccca
     3661 caggttcctc ccccgacgcg aactcgcgag cgagatcgag tgggccaaga acaggatgat
     3721 cgagcccgcc cgctacctgg cagagctcgg cgaccatgag ccgccgatcc ccgcggagct
     3781 gatgctgcgg atctacgacg gctacgagcg ccgcaagcgg gcgatgggtc ggctggattt
     3841 cgaggacatg ctcacactcg ccctccgact cttcgacgac catcccgatg ccgccgccgc
     3901 cgtcagagcc cggttctccg cgttcaccgt ggacgagttc caggacgtga atctgctgca
     3961 ggcgggattg ctcgaccggt ggctcggcga ccgcgacgac ctctgcgtgg tgggcgacga
     4021 ttaccagacg atctacgcgt tcacgggggc gtcacccgat cacctgctgg acttcacgcg
     4081 tcgattcccg cgcgcgacgg tcgttcgcct cgaagagaac taccggtcct ccccggagat
     4141 cctcgagctc gcgaaccggc tcgcgccccg actgggcggg atccggaaga ccctccgacc
     4201 caacagacca tccggtccgt ccccggtcgc ccgagccgag cccgatgagg gcgccgaggt
     4261 caccgccgtc gtggatgcgg tgcggcggct gcacctcgag gaggccgtac cgctcgagga
     4321 gatcgcggtg ctctcgcgga tcaacgcccg atccgagccg ttcgaggaag cgttcgccgc
     4381 agcgggcatc ccgtatcagg tacgcgacgg agcgttcctg cgacgacccg gaccgcgcgg
     4441 agtggtgcga cggttggagc gagcgagcgc ggacgccgcc gtggccgagg ccgtcgtcgg
     4501 catcaccgcc cagctcggat acgaccccga ggcgtcaccc gatgccgacg aggaggtgac
     4561 ccgccagtcc gacctcgcgc gcatgcgttc cctggcggcg gagttccagg ggacacatcc
     4621 cgccggtggc ccggcggcgt tcctcgccga gctggccgag cggttctcca ccgcgcacag
     4681 cagccgtggc gtgaacctgc tcacctatca ccgagcgaag gggctcgagt tcgatgccgt
     4741 gttcctccca cggatgatcg acggcgagct cccgttccgc tcgggcagag ccaaggccga
     4801 ccctcgcgag gagcgacggc tgctctacgt cgggatcacg cgcgcccgcc ggtacctctt
     4861 cctcacctgg ccccgaagcg ggcgatcgcg gccgagcccg ttcctcgacg agatggagct
     4921 gtcacccgct gggcttcccg ctcggacctc gtcgccgggc gccccggtcg ccgcgacccg
     4981 cggcggtccg ttgttcgatc gtctgaagga ctggcgccgc aagcgcgcgc aggcggacgg
     5041 tgttccggcc tacgtcgtgt tccacgaccg caccctcgcc gagatcgcgg aacggaagtg
     5101 caaggactgg gcagacctcg cggcggtctc cggcgtcggg ccagccaagc tcgagcggta
     5161 cgcggacgag atcctggcgg tcgtcgcggg aagctaggac gagagctccg cggcccgggg
     5221 gaagggcttc gcgccgatca actccagcat ctcgaggtcc tcgtggacgg gctgctgcac
     5281 gagcatacgg tccatgccgg cctcggcgta ctccgtgagt cctgcgacgg tcctcctctc
     5341 atgccaacag ggtcgtcgag aacgggtacg ggcacagcac ccggcagccg tccgacgtga
     5401 ccacgacctg atcgccgagc ttcaccccgt cgcggccccc cggctcaccg gcgtacagct
     5461 ccaccaccag ggccatgtgc tcttcgatga ccgtgtccgc gttcgactgg cgatcctgtg
     5521 ggtggcacac gctcgggttc tcctcctcga ggccgacgcc gtggaccatg cactcgtagc
     5581 gctgggcaag gtagcgctcg ggcatcggcg gggcgagcgt cgcgaggtcc ccgcacgtca
     5641 cgcccgggcg gatcagctcc tccatcccac ggagccactc gtgcgacgct cggtaggcgt
     5701 cgcgttgggc ggtcgacgga ggcgtgtctc ccgcgaggaa cgtccgcgac acgcagaaga
     5761 acatcccctc gatgccgatc gtgtcggtgt cgacgtagac gaggtcgccg gactcgagtc
     5821 gtcggtcggt cacctcggcc cgccatgggt tcgtgttggg cccgctgcac accgtgttcg
     5881 tggcgaggta ctcgccaccc ccgcggatca tgacgccggc gagcaccgcg agcaggtctc
     5941 gctcgctcac gccaggcgcg atcgcccggt cgagcgcggc gagcatctcc atcaccaatg
     6001 cggcgttctc gtcgagcaac cgaagctcct ggggcgtctt cacgcggcgc gcatcctgcg
     6061 tcaccggcgc cgagtcgccg accccgatcc cacgcctcgc gagcgccagg tatgcggggg
     6121 cgcccagccg gtccacggcg accgtcgatc cttcggcgcc gacctctcgg acggcttcga
     6181 ggagttcgtc tgcccacgca tcggcctcct tctccgggtc gtcgaagaac tcccacgcgt
     6241 gcatccggcg cacgtcgggg gcgcgcagcg ccgagcggtg catcgagttg ccgtgctcga
     6301 agaggatcgg tgttccttcc gccggcacga gggcacaccg gacgaacgtg ctcatcgcgt
     6361 agacgggcat cgcgctcgca cccgttgcgt agcggatgtt cggctcgttg aagaagaggc
     6421 agacctcgac atcgtgcgca tgcatcgccg cctggagtct cgcgagccgt tcgaggcgga
     6481 gggcctcgac gtcgtaccgc tcgttcgtcg cgacatcgga cacggcggat ggatagcacg
     6541 gatcggcgga ccgcgcccct atcgttggga catgcgggtt cctgtcgagg cgccgtggtc
     6601 gaggtggtgg accgtggatg ccctcctgta cgggtgctcg gcgctcttcg cgggactgac
     6661 cgccggggcg gcggggatcc ccctccagcg gtcgtggggg cgggtcgctc tttggcccta
     6721 cgcgctcgcg gcggcgatcg cgctcgtcgc tgcccggatc tccgggagcg agcagcagca
     6781 gcgccgtcgc cgaacgttcc tggtcgccgt ggtcttcgtc gcggccacga tcgcaccgat
     6841 ggcagtggcg gcgagccgtc gcgccggcgg tgaccccggc gcgtacgcgc aatccgaggt
     6901 gatcatcgtg gaggaggcgg cgatcgcgct gctcgaagcg agggacccct acgtcgccga
     6961 gtatcgcgac ggaccgctcg cggaacggcc cgtggcgacc caggtccact tcccgtacct
     7021 accggggatg ctcgcgttcg gcatcccgag ggctctggcc gatcccgcca cgtggacgga
     7081 tgctcgggtc tggttcacgg tggtggccct cgcggtcgcg ggcatggcac tcctgcgaag
     7141 ggcggtcgcg cccgaggacc gtctccgggc gttccagatg ctgttcgcgc tccccaccgg
     7201 cgcgctgctc ctcgcgaccg gaggggtcga catccccgtg atcgcgatgc tgctcggagc
     7261 cgccgtcctg gcgcggaacg accggcccgt cgcggcgggc gtgatgggtg gactcgccct
     7321 cgccacgaaa cagacctcca tcctcgtgct ccccttcctc gtgctcgcga tccctcgagg
     7381 acgtccgcgt cggcggttcg tcatcacggt cggcgccgtc ggggctgccc tcatcgtccc
     7441 gttcgccctg tgggacgtgg atgccttcgc ggaggacacg atcctgttcc ccttgggcct
     7501 cggacagggg gagtccgcgg ccgcgacgcc gacgttgggt tccctgctgg tcgatctctt
     7561 cccgtcatct cggaccgccg tgacggtgct gctggtgacg gcgatcgtga tcatcgttgc
     7621 atccctcctg ttccgggacc gcgggccgtc cctgtcgcag gcctgtgctc gctcaggcgc
     7681 cgccttcgtc gccgcgatcg cactcgcccc ggcggctcgg gtcggctacc tcgtctaccc
     7741 cgtcaacctg atcgtctggg cgatcgcgtt ccggcctccg tcggccggag gacggggccg
     7801 gtttcggcga caggtcctcc gggaaccatc cgcgggatga cgcaggcgga tcgaaacggc
     7861 cagggatctg gccagggatc tggatggagg acctgctcac gtggtcacaa gtaccggggg
     7921 gcgggcgggt gccccaccag atgggcgggg aacgacccgg gaggtcgaac gaagaagacg
     7981 gtccggtcca gccgccggcg caccggcacg cggcgctccg gaagtcgtca ctcgagggct
     8041 gacgcctccc gctagtgcca gccgcgggcg cgcccggcgt cggcgacccg ctcgaccgcg
     8101 atctcgtagg cggccgcccg atgcgagacc ccgccttcgt cggcgcgctc cagcacgagg
     8161 tcggtggccg attccatcag ctcctggagc cgtccgttga cgcgctcctc ggtccatcgc
     8221 tcccgctgga ggttctgccc ccactccagg tagctgaccg tgacgcctcc ggcactcccc
     8281 aggatgtccg ggatcaccca gacgccgcga tcgtgcagca ggcggtcggc gtcgggcgtc
     8341 gtggggtggt tcgcgatctc caggacgagg ttggcccgga cccgctcggc gttgtcgacc
     8401 cggagcgact cctcgagcgc agccggtgcg aggacctcgc actcggtgcc gatcaggtcg
     8461 tcgccctcga tcgtgtcgcc gtgcttgaag cccgtcacgc tcccggtctg ctgcttgtgg
     8521 cggagcacgt cgcgcgggaa cagcccctcg ccgttgacga cggcgcctcg ggagtcggac
     8581 acggccacga cgcgataccc cagctgatgg gcgagctccg cgaaccaggc gccggcgttg
     8641 ccgaacccct gcacggcgat cctgatgtgc tcccgcgtcc agcggcggga acgcgcgatg
     8701 cggtcgaggg cgaacaacgc tccccgtccg gtcgcggcct cccgtccgag ggaccctccc
     8761 agcttcaccg gcttgccggt gatcaccgcg ggccagtcac cacgagagcg cgagtactcg
     8821 tcgtacatcc acgccatctc gcgcggaccg gtgttcacgt cgggcgcggg gatgtcgcga
     8881 tagggcccga tcacgagttc gatcgacgag agatacgagc ggcacaggcg ttcgagctcc
     8941 gctaccgaga gactcttcgg gtcgacctgg acgcctcctt tcgcacctcc gaaagggacg
     9001 tccagcagcg cggtcttcca cgtcatcagg gcagagaagc atcggaactc gtcccgagag
     9061 gcgttcggat ggaaccgcag cccgcccttg aacggacccc gcgcaccgtt gtgctggatc
     9121 cgccatcccg gatacacgtc gaaggagccg tcgtcgcgag gaacacgaac cgacaccgcc
     9181 acctcccgac ggtgcgtcga caggaccttg cgggctccgt cctcgaggcg cagccgttcc
     9241 gcggcccctt cgagatgacc ctggaccgcg tcgaacaagc gttccgcgga gacggcgtgg
     9301 tcatgcgcgt catccgtcat accggcgagt ctttcatgac gtggacgtgc gcgcgtacga
     9361 ctgcgcggca tcctcgagct cctagccccg acgactgccg catcctcgcc ctgccgcacc
     9421 cgggtccggg cccgaggcct cggggaagat gcccagcgac gaagtccctc acgctcgaac
     9481 gtccgattgc tggcccgctg acacccccct acgatgcgcc tgtcagcaac cggccgaacg
     9541 gcggccgaga gagggattcg acgaccaggt gctctccttc ggtgtgacgt tcatgctcga
     9601 ccccccggtc tcgagaacga tcgagtgggc caagctcgcc gaagcgcagg ggttcgacgc
     9661 cgtgtgggcc tgggattccc acgtgctctg gcaggagttc tacacgacgt tcgcactggt
     9721 cgcggcgaac accgaacgcg ttcggctcgg gccgtgcgtc acgaaccccg cgacccgcga
     9781 catgacggtg accgcgagcg cgatggcgac gctccaggag gtgtccgggg ggcggatgga
     9841 catcgggatg ggtcgaggtg acagcgcgcg gcgcgtgatc ggacagaagc cggtcacggt
     9901 cgagcatatg gagcacgcct gtcggttcat caaggatctc gcggaagggc gagaagtcga
     9961 ccacgagggc acacctctcc agctgagatg gagcgagggc catcgcctgc cggtgtgggt
    10021 ggcggcgtac ggcccgaagg cgctccgcgc gtgcgggcgg gttgccgacg gcctgatcat
    10081 gcagctcgcg gacccgttca tcctcggatg gtcgctcgcc tacgtccgtg agggggccga
    10141 ggaggcaggt cgatccttcg aggacatcca gatccaggtg gccgcacccg ggtatctcgc
    10201 cgaggacctc gcgatggccc gcgatcaggt gcggtggttc cctgcgctcg tctcgaacca
    10261 cgtcgtggac ctcgtcaagc gatactccga cgccgacctt cctcaagcgt tgaccgacta
    10321 catcgaggct cgagatcact acgactacgc cgaccacggg cggaccggtg ccgagcacgc
    10381 agacttcgtg accgacgagg tcgtggaccg gttctgtgtg ctcggcaccg ccgacgcgtg
    10441 catcggcaag ctgcgcgaac tcgaggcgct cggcgtgacg cagttcaaca tctattcgat
    10501 gcaggaggat cccggcccgg ccgggatcat cgaagggttc gggaaggacg tgctaccggc
    10561 gttccgcacc tgacgcggaa caggatccag agaagcagag ggaggcagga acgatgacga
    10621 tggtccgggc ggctgtgata caggcgcacg cgaacatgcc gaaggacgac gcggtcgaca
    10681 agcacgtcga gctgatcgcg aaggcggctg ccgacggggc cgaggtcacg tgcctccagg
    10741 agatcttcca cggcccctac ttctgcgcgg agcaggaccc caagtggtac gcgaccgccg
    10801 aaccggagga tgggccgctc gtcacgcgca tgcgcgaggt cgccaagacc cacaacatgg
    10861 tcctcgtcgt cccgtggttc gaggaggcgc agaccggcgt gtactacaac tcggcggtcg
    10921 tgatcgagcg ggacgggacg atcgcgggca agtaccgcaa gacccacatc ccccacgtcg
    10981 ggccctgctt ctgggagaag ttctacttca agcccgggaa cctcggatat cccgtcttcg
    11041 atacgtcggt gggcaaggtc gggctgatca tctgctacga ccgccacttc cccgagatcg
    11101 gacgggccct cggcctcaag ggcgcggagc tggtgttcaa cccgtcggcc acggtggagt
    11161 cgttgtcgaa gtacctctgg gagctcgagc aaccggcgca cgcggtggcc aacggctact
    11221 ggatcgcggc gatcaaccgt gtgggcacgg aggaaccgct ctccagcgcg aagttctacg
    11281 gatcctccta cttctgcgac ccccgcggcc agatcgtggc gcaggcttcc gactcggagg
    11341 acgaagtgct ggtgcatgac gtcgacctgg acatgatccg ggacgtccgc aacacctggc
    11401 agttcctacg cgatcgccga cccgagctct acggggagct cacccagctg ctgccatgac
    11461 ggaggtcacc gcatgacgat gccccggtcg aaggtggacg gatgaaggga cgatcgtgag
    11521 gaccttgatc cagggcggca cggtggtgac agcgatggag acagtggctg ccgacgtcct
    11581 ggtcgacggg gagaaggtcg cggcgatcgg gaccggcatg tcggtcgagc cggaccgaac
    11641 catcgacgcg accggtcggt acgtgatgcc cggcgcggtc gacgtgcaca cgcacatgga
    11701 tcttccgttc ggcgggagct tctgctccga cgacttcgcc accggctcgc gcgccgcggc
    11761 gttcgggggc acgaccacga tcgtggattt cgcgctgcag gactacggcg agagcctccg
    11821 cacggggctc gatcggtggc acgagaaggc gcagaagtcc tccatcgact acggcttcca
    11881 catgatcgtc cgcgaggtga acgaccaggt cctgcaagag atggacacgc tcgtgggaga
    11941 aggcgtcacg tcgttcaagc tcttcatggc ctatcccggc gtgttcatgc tggatgacgg
    12001 cgccatcttc caggcgatgc agcgagcggc cgactcgggt gcgctgatca tgatgcatgc
    12061 cgagaacggc gggccgatcg acgtcctgat ccggcagtcc ctcgggcagg gcaggacgga
    12121 tcccgtgaac cacagcctga cgcgtccgcc cctgatggag ggggaagccg tccaccgcgt
    12181 gttcaagatg gccgagctcg cgggcgctcc ggcgtacatc gtgcatcttt cgtcgaggga
    12241 tgcactgaac gaggtccgcg aagctcggga ccgcggcgtg gcggcgtacg cggagacctg
    12301 tccccagtac ctctacctct cgatcgacga catggcgcgg ccgggtttcg agggcgcgaa
    12361 gttcgtgtgc tccccgccgc tccgcaccgc cgaccatcag gaggagctct ggaaaggact
    12421 gctggccgac gacctccagg tggtctccac cgaccatgcc cctttcaact tcaaggggca
    12481 gaaggatctc gggaaggacg acttctccaa gatccccaac gggctcccgt cggtggagga
    12541 ccggttcacg ctcctctatc aaggagtggt ggacgggagg atcggcctga accggttcgt
    12601 ggagatcgtc gcgaccgcgc ccgcgaagat gttcggcctt tccgcgcgaa agggaacgat
    12661 cgctcctggg ttcgacgccg atatcgtgat cttcgacccg gaccacgagc gcacgatctc
    12721 cgccgagacc caccacatga acgtcgacta ctcctgctac gagggacgcc gggtgaaggg
    12781 gctgccgcag atcgtgctcc agcgcgggag cgttctcgtc gacaacggcg agttccacgc
    12841 gaaggagggg caggggtcgt tcctgccgag gagccggatc tcgacatgac gatcgacgtc
    12901 gagcgacttc gtcggcttcc cctcttcggg gagctggatc accacgacct cgcgatgatc
    12961 gcgcatcacg tcggggagat ggaggcctcc gaagggcagg tgctcttcga gcagggctcg
    13021 atcccgtacg agatgttcgt gatcgaggag ggcaccgccg acgtgacgag ggacggccaa
    13081 acggtgggca ggatcggacc cggtgacgtg gtaggcgaga tcgcgctcct caggttccgg
    13141 cgccggatgg ggaccgttcg cgtcacgtcg ccgctccggg ccgtcacggt gagcgcggac
    13201 gaactccagg tgatcgagga ggagatgccc gagatcgccg ggaatctgcg gtcgatcatg
    13261 accgcgcgcg agcgcgagat cgccgatcga gccgacgact gacatgacga ccccccggat
    13321 gagctgatgt cccgcgaccc cgcgcaggac cgcgccgccg aggccacgcc cgcggtggat
    13381 cggcggcggc gagccgccct cctccgacag gcgcgctccg tcgctcgaca agcgaaccga
    13441 cgtgcacgct tctaccgcaa cgaccttccc cacgtgctgc gcgagcgcgc gcgtctggcg
    13501 gcgctgacgg gccgccctcg acgtgcccgc gcactcttct cccgccgcat cgaggtcgcg
    13561 accgatcaac gggctcattc ggagacgctc cgcacccgga tctctctcgc agtcaggcgg
    13621 gtggcgcgtt cggatggacg gcgttcgtcg aggacgggga acgggcgacc gtcgagctcc
    13681 gagctctcat cgcggcggct gctcgcgtgc ccatgaccga cggtgccacg tcggaggacg
    13741 tcgaaggaca cccggacgac ggatccctct cccgcgtgcc cgagccgacg accggagccg
    13801 tcgcggagac ggataccccg atcgacaggc gcgcgatgtt ccgggtaccc actccgtaag
    13861 gaccccgccc ggagcgccgc ggacaccccg ggatcttcac ggatccttca cggatttctt
    13921 ccgatccgac gtttgcgcgg acctcggagg ggtaaacagg gcgcaacggc gtctcggctc
    13981 ctccggccga ggcccgtcct cagatgtgtg gagtcggagg gtgccgggga acccaccccc
    14041 ttcccggtcc ctccgacccg catagatggc caccgcaagc caccgacgtc cagggatcgc
    14101 cctcgatccg acctcacccg cacgagagcc ccggtcctcg accgcatccg acctcgaggg
    14161 taggctcccg ctgactcgag tcgggaggac gggatgaaga caccgaggtt ctggcgggtg
    14221 cttggaagcg gagcgctcgc gctgctcctc gcgggttgct tcaaggtcaa catggaccta
    14281 gacgtgaccg ccgacaacac cgtctcgggg accgccgtgg tcgccgtgga ggagagcgtg
    14341 ctggaactga gcggtcagaa cgtcgatcag ctcttcgcgg acatggacct ctcggatctg
    14401 ccccccggct cgaaggccga cccgtacgaa gaggacggct tcgtcgggcg acagatcacg
    14461 ttcgaggatg tgcccctcga tgcgttcaca gggaacggga ccctctccgg cacggcatcc
    14521 ggggaggagc tggacatcgt gcgcgagggt gatgagttcc tcgtcaccgg cgggttcgac
    14581 atgagcggac aggaattcac cgggaccgag atcccgcagc agttcctcga gaacttcgag
    14641 ttccggatct cgatcacgtt cccgggaccg gtgagctccg cgagcggcga catcgacggg
    14701 aacaccgtga cgtgggagcc gcgggtcggc cagaacacgg agatccaggc ggtggcgagc
    14761 gcgatcccgt cttcgagctc accactcctg atgatcgtcc tgatcgccgc gggcgcgctc
    14821 gtgctcggcg cgatcgtcta cttcctcacc catcgcggag cggcggcggc cgacccgatc
    14881 gcgggtaccg ggttcggcga cgccgtcccc gcgcccgtgc cggcgcgccc tgaggcatcg
    14941 ccgatcgacg tcccgccgac cgcgttcccc gctccggacg atgcctctcc cgtcgacgac
    15001 acaccaccac ctccgcctcc gtcgagcggg agctgatcga attgcccacg tacgagtacc
    15061 gatgtggatc gtgcggcgcg accttcgagt cgcgacgcac gatcgcgcac gcggatgcgc
    15121 cgatcctctg ccctcacggc catgggaacg ctgtgcgacg accgtcggtg ttcgcggtca
    15181 cgtcgtccgt cacggccgaa cgtgccgctc gacctgcagg aacgggatgt ggtcccgact
    15241 gcgcccgcgc cgtgaacggt tgagccagga ccgatggtct agcgcatacg cactaccgtg
    15301 aagatgtcgt aaagggttcg cggaaccggt tgaaatctcc gcggccgctt cgatagcgtc
    15361 gacgtcgtcc gatgacctat cggagactct ctctcctcgt gttggtcgtc gccgtgctca
    15421 tcgcggggat gccttccccg gccggcgccg acaacatcac cgcccgccgg aagatgctcc
    15481 acctcctgaa ccagacgcga ggcaatcacg gcctctcagc gctccgactg aacgcgaacg
    15541 tctcgcactt cgcgtggaag cactcgaaga agatggcggg gcggaatcgc tgcttccata
    15601 cggcgaacct ctatgacgtg gtccgaacgt acagcccgtc cacgtggggc gagaacgtcg
    15661 gagcggccgg cacactgctg cggatccgga ccttgtggat gggaagcggg ggacaccgag
    15721 ccaacatcct gaaccctcgg ttccggagga tcggcatcgg ggtcgtgaag gcacggggcc
    15781 tcttgtgggt gaccacgatc ttctacgggg gctgacgccc tcaggacacg agtgaacgga
    15841 gctggcggag ggcgacggtg agctgagaga ggtcggtgac gccctccatc gcgagtgatc
    15901 gcatgaaccg ctgcacccgc ccgtaggcgg cgctccgatc ctcgaggaac gcatcgacgg
    15961 cttcatccat cggccggccg cccgcatgtt cgagcaccgt ctcgatgatc tcgcggcgga
    16021 tcgtgtacag atcgtcctcc atcgagtgaa ccgcccatcg ctgccaccgg gtcttcgccg
    16081 gctgctcggc gagccgctgc tcgagccaat cgatgtccag ccgctcaccg aggaggaaga
    16141 agccgcgcgc cacctcgagg accggccgcc cggtggcacg cgacaccgag atgatgtccg
    16201 ggccgtggac cagttcgtcc tggaacgcgt gtctgcgcgc aagctcgtcg gggatgcctt
    16261 cggcggtgag gcgcgtcgcc tcccgctcgc gctgctcgcg ccacgcctcc ggaccgatct
    16321 gctcgatcac cgacgacagc tcgcggaagg cggtcctcgc ctgaccgacg gcctcgccga
    16381 gccgtagccc cggcgcgcgg acgaggtacc agcgcgcggt cgactcgacc agcctgtcga
    16441 ccccgatcat caggtcgttc tggatctccg ggtcgatctt cccgtcgagg gactccaccg
    16501 cgtcccatcg cggcaccgcg cccacgacgt cgcgggcgat ccggaacgcg cgcacgacct
    16561 cggcaggggt cgcccccgtc tccgcgacga gccgcgacac gaatgtgacc ccttgggagt
    16621 tcacgacgtc gttcgagacg atcgtcgcga taagctcccg ccgcagcgga tgctcggtca
    16681 gcatcggccg gaaccggtcg acgatcgtct ccgggaagta ccgcgccagg tcctgttcga
    16741 ggtactcggc gtcggggagg tccgcctcga ggaggtcacg cttgatcgag cgcttcgagt
    16801 acgcgacgag cagcgcgatc tcgggccggg tgagtccctc accgcggccc cttcgctccg
    16861 ccatctcgtc cgaggtcggc aggaactcca cgagacggtc cagctcccct tcggcctcga
    16921 gctgaagcat caggtcctcg caggtctcgt tccgctctgc ggcgaccgcg acctcttgcg
    16981 agaggatctg cgcctgcaga tagttgtcgt agagcacacg acggacgacg tcgtccgtgg
    17041 aggcctccat cagggcgttg cgttcatcgg tggtcagctc tccgcgctcg atcgccagac
    17101 cgaggaggat cttgaggttc acctcccggt ccgaggtgtc gacgccggcg gagttgtcga
    17161 tgaagtccgt gttgatccgt ccaccggtga gcgcgtactc gatccgcccg cgctgtgtga
    17221 agcccaggtt gccgccctcc cccaccacgc gggcccggac ctggctcccg ttgatccgga
    17281 cggggtcgtt ggcgcggtcg cccacctcgg tgtggccctc gttcgccgcc ttgacgtacg
    17341 tcccgatccc gccgttccag agaaggtcga caggtgcctg aaggacccag cggatcacgt
    17401 ccgcgggcgt catctcggca ggtgtgtcgt cggggatcgc gagggcctcg cgcgcctggg
    17461 ccgacaacgt cacccacttc gccgtgcggt cgatcacctc ggccccctcg gagagcacgc
    17521 tcctgtcgta gtcgccccac gaggatcccg gcgtcccgta gaggcgctta cgctcggcga
    17581 acgagatcgc cggatccggc tgcggatcga ggaacacgtg tcggtggtcg aacgcggcga
    17641 ccaaccggat ccgatccgtg tagagcatgc cgttgccgaa gacgtcgccg gacatgtcac
    17701 cgatcccgac caccgtgaac ggctccgtca tcgggtcggt gcccagctcc cggaagtgcc
    17761 gcttgaccga ttcccacacg ccgcgagcgg tgatgccgag ctccttgtgg tcgtagccgt
    17821 gggagcctcc ggaggcgaac gcgtcaccga gccagtaccc gacccgctcg ctcacgccg
//
LOCUS       DASUYD010000086         6401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_928016, whole genome shotgun sequence.
ACCESSION   DASUYD010000086 DASUYD010000000
VERSION     DASUYD010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928016"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1926)
                     /locus_tag="VF195_05105"
     CDS             complement(<1..1926)
                     /locus_tag="VF195_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003888341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1989 domain-containing protein"
                     /protein_id="HEX6580231.1"
                     /translation="MVVGSGPSKLVVGGFRAADPSFEQYWVRPGAATVIAIAPEDRFT
                     VIDVDGGQVAELTVLSPDGTDAADAIGVRADAPATVLRALVSSTVGGAQEVVQDLAGR
                     GLDPSRGMAIRLFGEWSPPGSEQSFRADRESVLIVAAPAGRVVDGAPPPSDLVVEVHR
                     ARPRPPEEVELPPPLAEPRLDFRIDRSTARSYEVKAGEFIQVIDVEGKQCSDFLAFHR
                     HKLDDGLERGLDATVTRTLMGNAYPTPGLSGKFYDADMDPLVEVVRDTVGRHDTFALA
                     CNAKYYEDMGYPGHVNCTDNFNATLTVYEIAKRKGWPALNFFFNTAFNADNVLVSDEP
                     WSRPGDYVLLRAMTDLVCASSACPDDVDPANAWNPTDVHVRVYSPEHRFSAAIARRVT
                     PEARPVLTEKTGFHPRIEALTKNLVEYNGYWLPSHFSNEGAIAEYWACREHAAIMDLS
                     PLRKFEVLGPDAEALMQSTITRDARRLSVGQVTYTAMCNDAGGMIDDATVFRLGQDNF
                     RFVGGDDYDGVWLKEQAEQRRLNVWVKPSTDQLHNVAVQGPASREILDEVVWTPPTQP
                     PLAELGWFRFLVGRVQAPDGTPIVVSRTGYTGELGYEVWCHPDDADAVWDAIWEAGRS
                     RELTPFGLDALDIVRIEA"
     gene            complement(1932..3335)
                     /locus_tag="VF195_05110"
     CDS             complement(1932..3335)
                     /locus_tag="VF195_05110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017299861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6580232.1"
                     /translation="MSEKMWGSEPFWGLGYDWDPDWVYTDRQKELRETLIELCEKELR
                     ANAKRFDDELMFPRRNLELLGEHGFLGLTVPEEYGGLGENHVAYSMVCETIARYGCAS
                     TAMCYVMHIGAVNAIMLRATPELVDKYIRPLNSGTIGTLSYSDPETGSHFWYPVSSGA
                     ERANGGYKVRKKASWTTSAGFADFYVVQTTSPDFSGYDDLSVFVVDGDQVQSQPSLWD
                     ALGLRGNQSGPLEVPNVEIPATQIVGPEGDGANSNDESVDPWFLIGSSSVWNGLALGA
                     IDIAKRHTTRKKHVDVGLRVADYPTIQDYVGEALMDTNASRLFVLSVAQAMDRETDDN
                     TRNPEPGALARGNYLHWAWQIKFEAAKNVAHVVDKMLHACGGSGYKRDMELERYLRDA
                     KAGWVMGPTNEVLRQFVGKAVLLGFESLDYWNQSYNRRAVENEVKKLDAAGKRELAEQ
                     LLSEASERELKEPAKKA"
     gene            complement(3478..4992)
                     /locus_tag="VF195_05115"
     CDS             complement(3478..4992)
                     /locus_tag="VF195_05115"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6580233.1"
                     /translation="MAEAVIEERQLLKSLHWYDGFVIALCQPAFLLGSLGFTLATIGA
                     WGSVLLWGISATVGLLQNFIYSETAAMFPDKPGGIALYAHEGWRSRFSLFGPIAAFGY
                     WIGWSVVLSFVGNIVGALIVAEWFPESGDGTGDAYFNLGFADFGLAQAIAIVVILAVY
                     MFNVLGVKPSLAFGYVTGALLMVPLFIFIVMPYITGDWSSSNLEYVHNVGDPSLGFSD
                     LSIALMWLFIMCWSAYGIEVCASFAPEYHDTKRDTAKALKSAAVFSLFVYIFLPLGLG
                     GVVGSTGDYGSFYVAALNEIAGDVIGGFAVVCLIASLILAMNTATADGGRALYGIARD
                     DMTIKWLFHLNRFHVPARGMTVDMVVNIGLLLLLGANNFVILYISNIGYVFCHVLALS
                     GFLLLRRDRPGWPRPIKVGPAWLPIAALLALFNLVLVLYGIWDQDLAAYLGFYEFTGL
                     PLYLGLGVLVASVLLYFYRRVVQDKSKITFRDRDVPTMPNEEQMALLREESVSA"
     gene            5101..5457
                     /locus_tag="VF195_05120"
     CDS             5101..5457
                     /locus_tag="VF195_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580234.1"
                     /translation="MRGAPITIRCDCGQVERVPYGQRWECPDCGRRWNTEQIPAEEYW
                     GIMREMRRYRLQVVGVSLAFGVGAAIVLANMNVQRVLPIILGLMAFWFLVYMPRWRQK
                     VRRRARSLPHWQLHPE"
     gene            5461..5946
                     /locus_tag="VF195_05125"
     CDS             5461..5946
                     /locus_tag="VF195_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580235.1"
                     /translation="MTAGTRDLRTDVAAGVGSASRPVLLATFGVPLDEAASVFAVDTA
                     VESGQRLVLANLTTLQPLRMSLVLGYDTLPELTPDVTASLRRSAELARSLGVHVERLR
                     VRSPRPVEALLELVTELHPGLLVFGPDRSAMRGRRYRRSVRAVRERVTCLIWFPPDAV
                     G"
     gene            5950..6240
                     /locus_tag="VF195_05130"
     CDS             5950..6240
                     /locus_tag="VF195_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HEX6580236.1"
                     /translation="MTFLITCPNCGPREALEFSYGGETTRRAGPDASDAELAGYLFFR
                     ENANGWQTEWWVHRDGCRRWFLAERHTGTNEVRRTYWPSEREASPAGEATEA"
     gene            6237..>6401
                     /locus_tag="VF195_05135"
     CDS             6237..>6401
                     /locus_tag="VF195_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX6580237.1"
                     /translation="MTERLPERPGEAIGRDRPIAFDFEGRRVGAFEGDTVGSALAASG
                     VAITGRSFKYH"
BASE COUNT         1028 a         2157 c         2194 g         1022 t
ORIGIN      
        1 cgcttcgatc cgaacgatgt ccagcgcgtc caggccgaac ggcgtcagct cgcgcgaccg
       61 accggcctcc cagatcgcgt cccacacggc gtcggcgtcg tcgggatggc accagacctc
      121 gtagccgagc tcgccggtgt agccggtccg cgacaccacg atcggggtgc cgtccggcgc
      181 ctgcacccgg ccgacgagga accggaacca ccccagctcg gcgagcgggg gctgcgtcgg
      241 cggcgtccag acgacctcgt cgaggatctc gcggctggcc gggccctgga ccgcgacgtt
      301 gtggagctgg tcggtggacg gcttgaccca gacgttcagc ctgcgctgct cggcctgctc
      361 cttcagccac acgccgtcgt agtcgtcgcc gccgacgaac cggaagttgt cctggccgag
      421 gcgaaacacg gtggcgtcgt cgatcatccc gccggcgtcg ttgcacatcg ccgtgtacgt
      481 gacctgcccg accgagagcc gtcgcgcgtc ccgcgtgatc gtcgattgca tcagcgcttc
      541 ggcgtcgggg ccgaggacct cgaacttgcg cagcggtgag agatccatga tcgccgcgtg
      601 ctcgcggcac gcccagtact cggcgatcgc gccctcgttc gagaagtgcg acggcagcca
      661 gtagccgttg tattcgacga gattcttcgt gagcgcctcg atcctgggat ggaacccggt
      721 cttctccgtg aggaccggcc gagcctcggg cgtgacgcgg cgggcgatcg cggcgctgaa
      781 gcgatgctcg ggcgagtaga cccgcacgtg cacgtcggtg gggttccacg cgttcgcggg
      841 atcgacgtcg tccggacacg ccgacgatgc acagacgagg tcggtcatcg cgcgcaggag
      901 cacgtagtcg ccggggcggg accagggctc gtcggagacc aggacgttgt ccgcgttgaa
      961 cgccgtgttg aagaagaagt tgagcgccgg ccatcccttg cgcttggcga tctcgtagac
     1021 ggtgagcgtc gcgttgaagt tgtccgtgca gttgacgtgg cccgggtagc ccatgtcctc
     1081 gtagtacttc gcgttgcagg cgagtgcgaa cgtgtcgtgc cggcccaccg tgtcacggac
     1141 cacctcgacg agcggatcca tgtcggcgtc gtagaacttc ccggacagcc ccggtgtcgg
     1201 gtacgcgttg cccatcagcg ttcgcgtcac cgtggcgtcg aggccgcgtt cgagcccgtc
     1261 gtcgagcttg tgccggtgga acgcgaggaa gtccgagcac tgcttgccct cgacgtcgat
     1321 cacctggatg aactcgcccg ccttgacctc gtagctgcga gccgtggatc ggtcgatccg
     1381 gaagtccagc cgcggttcgg cgagcggcgg tgggagctcg acctcctccg gagggcgcgg
     1441 tcgggcgcga tgcacctcga cgacgaggtc gctgggcggg ggggcgccgt ccaccacccg
     1501 cccggccggc gcggccacga tcagcacgct ctcccggtcc gctcggaacg actgctcgga
     1561 gccgggtggg gaccactcgc cgaagagccg gatggccatc ccgcggctcg ggtccagccc
     1621 gcggccggcg aggtcctgca cgacctcctg tgcgccacca accgtcgaag agacgagcgc
     1681 gcgcaggacg gtcgcgggtg cgtcggcccg gacgccgatc gcgtcggccg catccgtgcc
     1741 gtccggcgac aggaccgtca gctcggccac ctggccgccg tcgacgtcga tcacggtgaa
     1801 ccggtcctcg ggggcgatcg cgatgaccgt cgccgcgccc ggccggaccc aatactgttc
     1861 gaacgacggg tcggccgcac ggaaccctcc gaccacgagc ttcgacgggc cgctgccgac
     1921 caccatcgcg cctaggcctt cttcgccggt tccttgagct cccgctccga ggcctcggac
     1981 agcagctgct cggcgagctc gcgcttgccg gccgcgtcga gcttcttcac ctcgttctcg
     2041 accgcccggc ggttgtagga ctggttccag tagtcgaggg actcgaaccc gagcaggacg
     2101 gccttgccga cgaactggcg gaggacctcg ttggtcgggc ccatcaccca acccgccttg
     2161 gcgtcgcgaa ggtagcgttc gagctccatg tcgcgcttgt agcccgagcc cccgcaggcg
     2221 tgcagcatct tgtcgaccac gtgcgcgacg ttcttggcgg cctcgaactt gatctgccac
     2281 gcccagtgca ggtagttgcc gcgggccagg gcgcccggct ccgggttccg cgtgttgtcg
     2341 tcggtctcgc ggtccatcgc ctgtgcgacc gagagcacga acaggcgcga ggcgttggtg
     2401 tccatgagcg cctcgcccac gtagtcctgg atcgtcgggt agtcggccac ccgaaggccc
     2461 acgtcgacgt gcttcttccg ggtcgtgtgc cgcttggcga tgtcgatcgc gccgagcgcg
     2521 agcccgttcc acacggacga ggagccgatc aggaaccagg gatcgacgga ctcgtcgttc
     2581 gagttcgcgc cgtcgccctc gggcccgacg atctgcgtgg ccgggatctc gacgttcggg
     2641 acctcgagcg ggccggactg gttgcccctc aggccgaggg cgtcccagag cgacggctgg
     2701 gactgcacct ggtcgccgtc gaccacgaac accgacaggt cgtcgtaccc ggagaagtcc
     2761 gggctggtcg tctggacgac gtagaagtcg gcgaaccccg ccgacgtcgt ccacgacgcc
     2821 ttcttgcgga ccttgtagcc gccgttggcc cgctcggcgc cggaggagac cgggtaccag
     2881 aagtgcgagc cggtctcggg gtccgaatag gacagggtcc cgatcgtgcc ggaattgagg
     2941 gggcggatgt acttgtcgac cagctcgggt gtggcgcgga gcatgatggc gttcaccgca
     3001 ccgatgtgca tgacgtagca catcgccgtc gaggcgcatc cgtagcgagc gatggtctcg
     3061 cacaccatcg agtacgcgac gtggttctcg ccgaggccgc cgtactcctc gggaaccgtc
     3121 aggccgagga acccgtgctc gccgagcagc tcgaggttcc tgcgcgggaa catcagctcg
     3181 tcgtcgaagc gcttggcgtt ggctcgcagc tccttctcgc agagctcgat cagcgtttca
     3241 cgcagctcct tctggcgatc ggtgtagacc cagtccggat cccagtcgta gccgagaccc
     3301 cagaagggct ccgagcccca catcttctcc gacatctcgt gtccttcccg gtggtggcat
     3361 cctcgagcgg gagtcacatc ctagtttcga aggctcgccc cgggcaacgc aggttcgaag
     3421 cacggaacgg gggcggccag gccgcccccg ttctccgatc cgagctccgc ggtggtgtta
     3481 ggccgagacc gactcctctc gcaggagcgc catctgctcc tcgttcggca tcgtcggcac
     3541 gtctcgatca cggaacgtga tcttgctctt gtcctggacg acccgccggt agaagtacag
     3601 gagcacggac gcgacgagga cgcccagtcc aaggtagagc ggcagcccgg tgaactcgta
     3661 gaagcccagg tacgccgcga gatcctgatc ccagatgccg tacaggacca gcaccaggtt
     3721 gaacagcgcg aggagcgccg cgatcggcaa ccaggcgggc cccaccttga tcggtcgtgg
     3781 ccagccgggt cggtcccggc ggagcagcag gaaccccgag agcgcgagca cgtggcagaa
     3841 cacgtacccg atgttcgaga tgtagaggat cacgaagttg ttcgccccga gcaggagcag
     3901 gaggccgatg ttcacgacca tgtccaccgt catcccgcgc gccggaacgt ggaatcggtt
     3961 caggtggaac agccacttga tcgtcatgtc gtcgcgggcg atcccgtaga gggctcggcc
     4021 tccgtcggcg gtggccgtgt tcatggccag gatgaggctc gcgatcaagc acacaacggc
     4081 gaacccgccg atcacgtcgc cggcgatctc gttgagagcc gccacgtaga agctgccgta
     4141 gtcgccggtc gagccgacca cgccgccaag accgagcggc aggaagatgt acacgaacag
     4201 cgagaacacc gcggcactct tcagcgcctt ggcagtgtct cgcttcgtat cgtggtattc
     4261 gggcgcgaag ctcgcgcaga cctcgatccc gtacgcggac cagcacatga tgaacagcca
     4321 catcaacgcg atcgacaggt cgctgaagcc cagggacggg tcgccgacgt tgtgaacgta
     4381 ctcgaggttc gagctggacc agtcgccggt gatgtacggc atcacgatga agatgaacag
     4441 cgggaccatc agcaacgcgc ccgtcacgta cccgaaggcg agcgacggct tgaccccgag
     4501 gacgttgaac atatagaccg ccaggatcac gacgatcgcg atcgcctgcg cgagcccgaa
     4561 gtcggcgaag ccgaggttga agtacgcgtc gccggtgccg tcacccgact cggggaacca
     4621 ctccgcgacg atcagcgccc cgacgatgtt gccgacgaac gagagcacga ccgaccagcc
     4681 gatccagtag ccgaacgcgg cgatcggccc gaacaggctg aatcgcgacc gccagccctc
     4741 gtgcgcgtag agcgcgatcc cgccgggctt gtcggggaac atcgcggccg tctcggagta
     4801 gatgaagttc tggagcagtc cgacggtcgc ggagatgccc cacaggagga ccgatcccca
     4861 ggcgccgatc gtcgcgagcg tgaagccgag cgacccgagg aggaacgccg gctggcacaa
     4921 cgcgatgacg aacccgtcgt accagtgcag ggacttgagg agctgacgtt cctcgatgac
     4981 ggcctcagcc acgacgatcc cctcccaagg catccgggtc gaacgccagc gaggggtata
     5041 gcactttggc ttcgggcgtt caaaacggcc cgatgtgcgt gggggataca atccggcccg
     5101 atgcgcggcg cacccatcac catccgctgt gactgtggtc aggtggagcg cgtcccgtac
     5161 gggcagaggt gggagtgccc cgattgcggg cgccgctgga acaccgagca gatcccggcc
     5221 gaggagtact gggggatcat gcgggagatg cgccgatacc gcctccaggt cgtcggggtg
     5281 tcgctggcgt tcggcgtcgg ggcggcgatc gtcctggcca acatgaacgt ccaacgcgtc
     5341 ctgccgatca tcctcggcct gatggccttc tggttcctgg tctacatgcc gcggtggcgc
     5401 cagaaggttc ggcgccgggc ccggagcctc ccacactggc agctgcaccc ggagtgatcg
     5461 atgaccgccg gcacccgtga tctgcgaacg gacgtagcgg ccggggtcgg ctccgcgagt
     5521 cgtcccgtgc tcctcgcgac gttcggcgtg cccctggacg aggcggcatc ggtgttcgcg
     5581 gtcgacacgg ccgtcgagtc gggccagcgg ctggttttgg ccaacctcac gaccctgcaa
     5641 ccccttcgga tgtccctggt gcttggctac gacacgctgc ccgagctcac gcccgacgtt
     5701 acggcgtccc tgcgtcgatc ggccgagctt gcccgatccc tcggggtgca cgtggagcgt
     5761 ctccgcgtga ggagccctcg tcccgtggag gcgctcctcg agctggtcac cgaactgcac
     5821 ccggggctgc tcgtgttcgg acccgatcga tccgccatgc gaggtcgtcg ctatcgacgg
     5881 tccgttcgcg ccgtgcggga gcgggtgacc tgcctgatct ggttcccacc cgacgccgtc
     5941 ggctgaccga tgacgttcct gatcacgtgt ccgaactgcg gcccgaggga ggcgctggag
     6001 ttctcctacg gcggcgagac cacacgacgg gccggcccgg acgcgtcgga tgccgagctg
     6061 gcgggctatc tcttcttccg tgagaacgcg aacggatggc agaccgagtg gtgggtccac
     6121 cgcgacgggt gccgcaggtg gttcctcgcc gagcggcaca cggggacgaa cgaggtccgt
     6181 cgcacctact ggccgagcga gcgggaggct tcgcccgccg gtgaggcgac cgaggcgtga
     6241 ccgagcggct tcccgaacgt cccggcgagg cgatcgggcg cgaccgtccg atcgccttcg
     6301 acttcgaggg tcgcagggtc ggcgcgttcg agggggacac cgtcggatcc gcgctcgcgg
     6361 catcgggcgt cgcgatcacc ggtcgctcgt tcaagtacca c
//
LOCUS       DASUYD010000087         4280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1368612, whole genome shotgun sequence.
ACCESSION   DASUYD010000087 DASUYD010000000
VERSION     DASUYD010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4280
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368612"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..618
                     /locus_tag="VF195_05140"
     CDS             82..618
                     /locus_tag="VF195_05140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580238.1"
                     /translation="MPQPLRLVVSRGLSPDESVSEAQSFEGFFEAESAAMFRRLCLVT
                     GNRHEAEEVMQDAFLNVFERWDRVRQMDDPTGYLYRIAFNVFKRRSRRAAMALRRTLG
                     VGEGNDEYAAADARFMVEQGLAELSPRQRAAIVLTELLGYSSEAAGKVLGVRPVTVRA
                     LASQGRAAMRRTLERDDV"
     gene            611..1198
                     /locus_tag="VF195_05145"
     CDS             611..1198
                     /locus_tag="VF195_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580239.1"
                     /translation="MSEFRQDLERRSERFELARGALDRLFERRRRKQRNQRIAAGAAA
                     LVVTAGMGLAIMGLRGLGTETKPAAIPSIGPNRPLYLEIAGTYTVTLPNDDPRVHDNG
                     LAGRYTMRLRPDGVVLLSVPQGFGREGPSPSGISFRLSGNQFTTNAFINLTCPQSVGV
                     YRWELMGRRLTFMPLEEDCEVRRVLFASEPWQVQP"
     gene            1268..1852
                     /locus_tag="VF195_05150"
     CDS             1268..1852
                     /locus_tag="VF195_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580240.1"
                     /translation="MRLDHRPIARGTFVAAAMSMVLAACAGRGATTGEEASRTPAVTT
                     EPTGQVDPIEGEWRADFTCKDSLRAIETRLSSKQIREQVGSLGSFIEGGGWGAEPTGD
                     DPCHAATQTVAALVRFADGDLALFDVVTGEGVQATYELVDDHTMVVNDTSGNLCPCPG
                     RWGFEIAGDEVTFRVEPDIFIIATWEAAPFHRVS"
     gene            1854..3374
                     /locus_tag="VF195_05155"
     CDS             1854..3374
                     /locus_tag="VF195_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6580241.1"
                     /translation="MHGPALTPLIGIAVLLLASCTESSEPEFRPTFQQGPCPDDVTHV
                     VLTPVTCGFITVLENRSEPGGDTIRLFVTRIEPPGGNPAPDPVLTLGIDLGDTPDYGG
                     FAPGAQRYNRELILVDQRGTGHSEPTLACPEVERLGEQLVGARLSDIEARADLQSAVE
                     ACHSRLTGEGIDLSAYNLGEIAADVEDVRRALGIPRWNISSYGSASRIALEVIRLSPD
                     HVRAMWFDTPQFPQVDELTLGISGTEHALSQVSADCAADPACSRRFPDLSNALQETLA
                     QLDEEPITATVESPSGAGGHSIRVVVDGGAFLRAVRAMVSDMELGMVRRVPAIIYGAM
                     EGRVDLVARLLSAEPLCLGYQPVCGDNPFFEGAFFSVLCHDEVPFVEPSRLTGMAGGE
                     PGFQEAYVASPYLDVCEAWDVGEATSVAHRPVSSDIPMLIYVGGYDAYSSLPDAEQAT
                     TSFSGSFLVDVPYQGHNVLGSLDCYRNIRNAWIEDPTSPPETGCIEEIGPPTFEAS"
     gene            complement(3420..>4280)
                     /locus_tag="VF195_05160"
     CDS             complement(3420..>4280)
                     /locus_tag="VF195_05160"
                     /inference="COORDINATES: protein motif:HMM:NF014803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX6580242.1"
                     /translation="LDAILPTGVHINVDLLSQLDRYGRRAPGSTVGIRVNPRAGAASR
                     PGLAGLYTGGHRPSKFGILQEQLDDALEIARTHDLTIDTVHLHVGRGVLTDALPAFEM
                     AVERAVPMAERLVAAGHPISEVNVGGGLGVPYDTGQEPLDVHAYADVLRRHLAPLGVT
                     VACEPGDFLTKDMGVLLAEVVSVDDRDGYRFVGVDAGFNVAPEHFIYEEPVPIVLSRA
                     VDGPPGRPVTVAGNINEGDDLWGEDVALPEPHEGDVLALLRMGSYNRSMHIDHCLRPP
                     AGTVAFADRR"
BASE COUNT          675 a         1430 c         1465 g          710 t
ORIGIN      
        1 ggaggggcaa ccatctgtgc catcaggcga gatcgctgac gcgctaaacg gaaccggctg
       61 tcgaggtctt ctcctatcag gatgccgcag ccgctccggc tcgtggtctc gcgcgggctc
      121 tcgcctgacg agtccgtgag cgaggcacag agcttcgaag ggttcttcga ggcggagagc
      181 gcggccatgt tccgacggct gtgcctggtc acggggaacc gccacgaggc cgaggaggtg
      241 atgcaggacg cgttcctcaa cgtgttcgag cgttgggacc gcgtccggca gatggacgac
      301 cccacggggt acctgtaccg gatcgctttc aacgtcttca agcgccggtc gcgacgagcc
      361 gcgatggccc tccgccgcac gctcggcgtc ggtgagggga acgacgagta cgcggccgcg
      421 gatgcaaggt tcatggtcga gcaggggctc gccgagctca gcccacgaca gcgggccgcg
      481 atcgtgctca ctgagctgct cggctacagc tccgaggccg ccggcaaggt gctcggcgtg
      541 cggccggtca ccgttcgagc cctcgcgtcc cagggccggg ccgccatgag acgaacgctg
      601 gagcgggacg atgtctgaat tccgccagga cctcgaacgc aggagcgagc gattcgagct
      661 cgcgcggggc gccctcgacc ggctgttcga gcgccggcgg cggaagcagc ggaaccaacg
      721 gatcgccgcc ggcgccgcgg ccctggtcgt cacggccggc atggggctcg cgatcatggg
      781 gctgcgcggc ctcgggacgg agacgaagcc ggccgcgata ccgagcatcg gtccgaaccg
      841 tccgttgtac ctggagatcg ccggcaccta cacggtgacg cttccgaacg acgatccacg
      901 ggttcatgac aacggccttg ccgggcggta caccatgcga ctccgacccg acggtgtcgt
      961 cctgctgtcg gtcccgcaag gcttcggccg ggaggggcct tcgccctcgg gcatctcctt
     1021 ccggctctcg ggcaaccagt tcacgaccaa cgcattcatc aacctcacct gcccacagtc
     1081 ggtcggcgtc taccggtggg agctcatggg tcgccggctc accttcatgc ccctcgagga
     1141 ggactgtgag gtccgccgag tcctcttcgc ttcggaaccg tggcaggtcc agccttgatc
     1201 tttcgtggct aaacggttct ggctcccacg ccttcttctc ttcggatcgc atctgaggga
     1261 gggagttatg cggctggatc accgaccgat cgcccggggg acgttcgtag cagcggccat
     1321 gtcgatggtg ctcgcggcgt gtgcggggag gggggcaacg accggggagg aagcctcccg
     1381 gaccccggcc gtcaccaccg agcccacggg ccaggtggac ccgatcgagg gggagtggcg
     1441 ggccgatttc acgtgcaagg actcgttgcg agcgatcgag acacgcctgt cgtcgaagca
     1501 gatccgtgag caggtgggaa gcctgggatc cttcatagaa ggaggagggt ggggggcgga
     1561 gccgacgggg gatgatccct gccacgcggc gacccagacg gtcgcggcgc tggttcgctt
     1621 cgcggacggg gacctcgcgc tgttcgacgt ggtgactgga gaaggggtcc aggcaacgta
     1681 cgagctcgtc gacgatcaca cgatggtcgt gaacgacaca tccggcaacc tctgcccctg
     1741 tcccggaagg tggggattcg agatcgccgg cgacgaggtc acgttccgcg tcgagccgga
     1801 catcttcatc atcgccacct gggaggcagc ccccttccac cgggtgagct gagatgcacg
     1861 ggcccgcgct gactccgctc atcgggatcg ccgtcctact cttggcttcg tgcaccgagt
     1921 cctcggagcc ggagttccgg cccacgttcc agcaggggcc atgtccggac gacgtcactc
     1981 acgtggtcct gacgccggtg acgtgtgggt tcattaccgt cctggagaac cgatcggagc
     2041 ccggggggga caccatcagg ctcttcgtta cccggatcga gcccccgggc ggtaacccgg
     2101 ccccggatcc ggtgctcacc cttggcatcg acctcggaga cactcccgac tatggcggat
     2161 tcgccccagg cgcccagcga tacaaccgcg agctcatcct ggtcgaccag cgcgggaccg
     2221 gtcactcgga gccgacgctg gcctgcccgg aggtggagcg cctcggcgag cagctggtcg
     2281 gggcccgcct ctcagatatc gaggcgagag ccgacctcca gagtgcggtg gaggcttgcc
     2341 acagtcggct gactggcgag gggatcgacc tgtcggcgta caacctaggt gagatcgccg
     2401 cagacgtcga ggacgtacga cgggcactcg gcattcctcg atggaacatc tcttcctacg
     2461 ggagcgcttc gcgcatcgct ctcgaggtca tccgtctttc cccggatcac gtccgcgcca
     2521 tgtggttcga cacgcctcag ttcccgcagg tggacgagct caccttgggc atctcgggaa
     2581 ccgagcatgc cttgagtcag gtctccgccg actgtgcagc cgatccggca tgcagccgcc
     2641 ggttccccga tctgtcgaac gccctccagg agaccctggc ccagctcgat gaagagccca
     2701 tcaccgcaac cgtcgagtca ccgtccgggg ctggggggca ctccatccgt gtcgtggtgg
     2761 acggcggggc gttcctcaga gcggttcgcg ccatggtctc ggacatggag ctcggaatgg
     2821 tgcgtcgggt cccggccatc atctacggtg ccatggaagg aagggtcgac ctcgtcgccc
     2881 gcctcctctc cgctgagccg ctttgcttgg gttatcagcc cgtctgcggg gacaacccgt
     2941 tcttcgaggg tgccttcttc tcggtcctct gccatgacga agtcccgttc gtcgagcctt
     3001 cgcgcctcac cgggatggcc ggaggcgagc cgggtttcca ggaggcatac gtggcgagcc
     3061 cttacctgga cgtgtgcgag gcctgggacg tgggtgaggc gacctcggtc gcgcatcgac
     3121 cggtctcgtc ggacatcccg atgctcatct acgtcggggg gtacgacgcg tacagctctc
     3181 tcccggacgc ggagcaggca accacctcgt tctccggatc cttcctcgtc gatgtgccct
     3241 accagggaca caacgtgctc ggctcgctcg attgctaccg gaacatccgg aacgcctgga
     3301 tcgaggaccc cacgtcgcct cccgagaccg gttgcatcga agagatcgga ccgccgacct
     3361 tcgaggcgag ctaagggggc ccccatgaac gacacgtgct cttcgtgatc gacctcgagt
     3421 tatcgccggt cggcaaacgc gaccgtcccg gccggcgggc ggaggcagtg gtcgatgtgc
     3481 atcgaccggt tgtagctgcc catgcggagc aacgccagca cgtccccctc gtgcggctcg
     3541 gggagcgcga cgtcctcacc ccacaggtcg tcgccttcgt tgatgttgcc ggccaccgtg
     3601 accgggcgcc ctggcggtcc gtcgacggct cgggaaagga cgatcgggac aggttcctcg
     3661 tagatgaagt gctcgggcgc aacgttgaac ccggcgtcga cacctacgaa ccggtagccg
     3721 tcgcggtcgt cgacgctcac cacctcggcg agcaacaccc ccatgtcttt cgtgaggaag
     3781 tcccccggct cgcaggcgac cgtgacgccg agcggggcca ggtggcggcg caggacgtcc
     3841 gcgtacgcgt ggacgtccag gggttcctgc cccgtgtcgt acgggacgcc gaggcctcct
     3901 ccgacgttca cctcggagat cggatgcccg gccgcgacga gccgctcggc catcgggacg
     3961 gcgcgctcga cggccatctc gaacgccggt aaggcgtcgg tgagcactcc ccggccgacg
     4021 tgaaggtgaa ccgtgtcgat cgtgaggtcg tgcgttcgcg cgatctcgag ggcgtcgtcg
     4081 agctgctcct ggaggatccc gaacttcgac ggccgatggc ccccggtgta caacccggcc
     4141 agccctgggc gtgaggcggc cccggcccgc gggttcacgc ggatcccgac cgtcgacccg
     4201 ggcgcccggc gaccgtaccg atcgagctgg gacagcaggt cgacgttgat gtggacgccg
     4261 gtcggaagga tcgcgtcgag
//
LOCUS       DASUYD010000088         2361 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_928736, whole genome shotgun sequence.
ACCESSION   DASUYD010000088 DASUYD010000000
VERSION     DASUYD010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_928736"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF195_05165"
     CDS             <1..467
                     /locus_tag="VF195_05165"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HEX6580243.1"
                     /translation="GDLQARVRGWVPRFIALFTLTAAISAVWIAVGDYGVTDTFTDDV
                     WTIVFPLGALVAYVVMIVMVRRGRDFAAFFSSSTVIAFSLATISAGLYPNMLKSSTNP
                     AYSMTVSNASADHYTLTVMLVVAIIGIPFVLLYTAGVQYLFSGKVKLTPSSY"
     gene            493..1542
                     /locus_tag="VF195_05170"
     CDS             493..1542
                     /locus_tag="VF195_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HEX6580244.1"
                     /translation="MSTTALMAVFVVGAGATWIAGVFLADTTDVVDDRFNLGEALGGL
                     ILLGFAGTLPEIAITVSAVVQGHLGLATGNLLGGIAMQTLVLVLLDATSRSKTPLSTL
                     STVLEPIIEAVLVIILVTMALLAPLLPASVAIGPVSPISLLIVITWFFGLLVLNHLRA
                     SERWTTVTAQVEAQMVAPSPGPVEGVRPNRFERSKTSLVVLAFALAAGVTLIAGVVLE
                     QSGNALANRWGINGVIFGATILAAVTALPEISTGIRAVRLGQVGLAMGDIFGGNQVQM
                     TLFLVADLLAGKPVLQTVSASSSWLGGIGVVVTALFAGGLVIRPPKKFVGIGPDSWVV
                     VFVYAIGLAGLLRIS"
     gene            1550..>2361
                     /locus_tag="VF195_05175"
     CDS             1550..>2361
                     /locus_tag="VF195_05175"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEX6580245.1"
                     /translation="MASSDFLPPSGEQHEIRHGDDVVVVTEVGATLRAYSAGGLDVLD
                     GFSMDEPSSAGRGQVLAPWPNRLDGGRYRFGGRQARAPIDEPELGNAIHGLVRWLPWL
                     LASKTDEAVALECVLHPQPAYPWRLELGLEYRLAGDGLEVVARATNASLEAAPFGIGF
                     HPYLTMGIPVDDVRLTIPASRRLTTDDRALPVGEEDVAETGFDFTVGRPVGVTQLDTC
                     FTGLAGGSDGRSRARLESTDRERDVEVWADEAFGYLQVYTGDTLEPASRRRQA"
BASE COUNT          338 a          786 c          815 g          422 t
ORIGIN      
        1 aaggcgatct gcaggcgcgc gtgcgaggat gggtcccccg gttcatcgcc ctgttcaccc
       61 tgactgcggc gatctcggcc gtgtggatcg cggtcgggga ctacggggtg acggacacgt
      121 tcacggacga cgtgtggacg atcgtgttcc cgctgggcgc cctggtcgcc tacgtggtga
      181 tgatcgtcat ggttcgccgc ggccgggact tcgcggcgtt cttcagcagc tcgacggtca
      241 tcgcgttctc gctggcgacg atctccgccg ggctgtaccc gaacatgctg aagtcgtcga
      301 cgaaccccgc ctacagcatg acggtctcga acgcttccgc cgaccactac acgctcacgg
      361 tgatgctggt tgttgcgatc atcgggatcc cgttcgtgct cctctacacg gccggggtcc
      421 agtacctgtt cagcgggaag gtcaagctca ccccgagcag ctattgatga cgaacggagc
      481 cgcatgggct cgttgagcac caccgccctc atggctgtct tcgtggtggg ggccggcgcc
      541 acgtggatcg caggcgtgtt cctggccgat accacggacg tcgtggacga ccgtttcaac
      601 ctcggggagg ccctgggagg cctgatcctg ctcgggttcg ccgggacgct ccccgagatc
      661 gcgatcacgg tctcggcggt cgtccagggc cacctcggtc tcgcgacggg gaacctgctc
      721 gggggcatcg cgatgcagac cctcgtgctg gtgctgctcg atgccacctc acggagcaag
      781 actccgctat ccacgctgtc gaccgtgctc gagccgatca tcgaggcagt cctcgtgatc
      841 atcctcgtga cgatggcgct gcttgcgccg ctgcttccgg cctcggtcgc gatcggcccg
      901 gtgagtccga tctcgctcct gatcgtcatc acatggttct tcggcctgct ggtcctcaac
      961 cacctccgcg ccagcgagcg ctggacaacc gtgaccgcac aggtcgaggc gcagatggtg
     1021 gcgccgagcc ccggcccggt cgaaggcgtc cgacccaaca ggttcgaacg atcgaagacc
     1081 tcgctcgtgg tgctcgcgtt cgcactggca gcgggagtca ccttgatcgc cggcgtcgtg
     1141 ctggagcaga gcggcaacgc cctggcgaac cggtggggga tcaatggcgt catcttcggc
     1201 gcgacgatcc ttgcggcggt caccgcgctc cccgagatca gcaccggcat acgggcggtt
     1261 cggctcggtc aggtcggtct cgcgatggga gacatcttcg gcggcaacca ggtgcagatg
     1321 acgctgttcc tggtggcgga cctgttggca ggcaagccgg tactgcaaac ggtcagcgcg
     1381 agctcgtcat ggttgggcgg gatcggcgtc gtcgtgacgg cgctcttcgc cggcggcctg
     1441 gtcatccgcc cgccgaagaa gttcgtcggt atcggccccg attcgtgggt ggtggtgttc
     1501 gtgtatgcga tcgggctcgc cggactcctc cggatcagct gagacgttga tggcctcgtc
     1561 ggacttcttg ccaccctcag gcgaacaaca cgagatccgc cacggcgatg acgtggtcgt
     1621 cgtgaccgag gttggcgcga cgctccgagc gtactcagcg ggcgggctcg acgtcctgga
     1681 cggcttctcg atggacgagc cctcgtcggc cggacgcggg caggtgctcg ccccgtggcc
     1741 gaaccgactg gacggcggtc gctataggtt cggggggcgc caggcgcggg cgccgatcga
     1801 cgagcccgag ctcgggaacg cgatccacgg gctcgtgcgt tggctcccct ggttgctcgc
     1861 gtcgaagacc gacgaagcgg tcgcgctgga gtgcgtcctg cacccgcagc ccgcgtaccc
     1921 atggcggctc gagttgggcc tcgagtatcg gctcgccggg gacgggctcg aggtcgtcgc
     1981 gcgtgcgacg aacgcctccc tcgaggcggc accgttcggg atcgggttcc acccctacct
     2041 gacgatgggg atcccggttg acgacgtgcg actcacgatc ccggcctcgc gcaggctgac
     2101 cacggatgac cgagcccttc cggtcggcga ggaggacgtc gccgagaccg ggttcgactt
     2161 caccgtcggt cgtccggtcg gcgtcacgca gctcgacacc tgtttcacgg gcctcgctgg
     2221 cggatccgac ggccggtcgc gagcccgcct cgaatcgacc gaccgcgaac gagacgtcga
     2281 ggtctgggcg gacgaggcgt tcggctacct ccaggtctac acgggggaca ccctcgagcc
     2341 tgcctccagg cgccggcagg c
//
LOCUS       DASUYD010000089         7620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1369622, whole genome shotgun sequence.
ACCESSION   DASUYD010000089 DASUYD010000000
VERSION     DASUYD010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7620
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1369622"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF195_05180"
     CDS             <1..606
                     /locus_tag="VF195_05180"
                     /inference="COORDINATES: protein motif:HMM:NF023367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3459 domain-containing protein"
                     /protein_id="HEX6580246.1"
                     /translation="GRFPTRWCDGREDRSRCALMVLLTLRGTPFLYYGDEIGMTDVAV
                     PEDDQLDLVGGGGAEQPSRDGCRTPMQWSADPSAGFTRDGVRPWLPIGDAASRSVAAQ
                     REDSGSMLNLCRDLIALRREVPDLRSGRYVSIPSPAGVWTWSRGDDTAVSLNLSEEPV
                     AVAIGTGTIAIGTRRERDGQPVGDELSLGPWEGAVLRLAPS"
     gene            complement(557..1138)
                     /locus_tag="VF195_05185"
     CDS             complement(557..1138)
                     /locus_tag="VF195_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580247.1"
                     /translation="MARSRRPAGAGAQPLSPRQVWRAILLSTLLLVPAYWGLLVGLVS
                     IASDDPERAPSAGPWIAFGLCLIPFVFLALAFLSQHPRAPRATVLAMILCIVVGIPVS
                     AVAADAVTGFVAGVGAGGIAAMRSDLDRAWRARALAIVIVSAWAFVTVRTVTDVALLL
                     APVLPFTSVGVADHLLEIRRERGAAPHPPTDRG"
     gene            complement(1146..2033)
                     /locus_tag="VF195_05190"
     CDS             complement(1146..2033)
                     /locus_tag="VF195_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6580248.1"
                     /translation="MEAAGRGGWFVRITIIVIVILWSIPTLGVLITSFRPEDAVNTSG
                     WWEVFAHPFGATEWTLENYRIALDQGGFGNAFLNSLAVTIPSTVIPITIAAFAAYAFS
                     WMEFRGRYVLFVIVVGLLVVPLQMALIPILRLYTQGATLFGREIFPDLSLQGTFLGIW
                     LAHAGFGLPLAVYLLRNYIGSLPSSIIESAKIDGADHFTIFWRLVVPLSVPAIAAFAI
                     FQFLWVWNDLLVAYVFLGGAGQNIVVTVALRELVGSRGENWHLLTSAAFISMALPLLV
                     FFALQKYFVRGLTAGAVKG"
     gene            complement(2093..3244)
                     /locus_tag="VF195_05195"
     CDS             complement(2093..3244)
                     /locus_tag="VF195_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018525682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6580249.1"
                     /translation="MSVTSEPASAPGLTGTPPSRRFTGVLVLLLRVLVAVAVPVATFY
                     ILWWTFEFLRDENANRFIVVGVAIAVGVGGVFVLYWGMNRAVDLFPARVRESVRPYVF
                     VGPCLVILAVFLVYPVINTVLISFKDANSQSFVGLDNFKFVFTDSDMLRAIRNTAGWI
                     ILVPLAAVTIGLIYATLADRLRRGEAVAKSLIFLPMAISFAGASITWQLIYSYRPQGF
                     GSNIGLLNGIMIGLGQEPVKWLELQPWNNLFLMVIMVWIQTGFAMVVLSAAIKSIPDE
                     IIEAARIDGASEFQVFRRIMVPSILPTIVVVTTYMVINSLKVFDIVFVQPASAETNGT
                     VVVAERMIRWFFQLNHNGRAAAIAVVLFAAVIPVMVWNIRRFREEEAIR"
     gene            complement(3366..4694)
                     /locus_tag="VF195_05200"
     CDS             complement(3366..4694)
                     /locus_tag="VF195_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580250.1"
                     /translation="MKPLRLIVPFAVFLLIAAACTSDDDGGGGETGNGPALEENTGTV
                     NVLNAMEPNEGEAVQAAVDENLQNADYAVEIEASADFEEQFAIRSEGGTLDIALVPQP
                     GTVQQQADAGTIVSLEDLGIDVGALQEAYGEYLFSLVEVDGEHYGIPTNVNLKSMVWY
                     PKDDFDAAGYQVPTTWDEMLALSDQIVADGGTPWCVGFESGGATGWPATDWMEDIMLR
                     TAGVDTYDQWVTHEIPFTDPAVAAAAEEFGNIMFTDGYVLGGAADTPSISFGSAPLPM
                     FEDPPGCWLHRQATFIIGAAPFPEDAEAGVDYDWFPLPPIDSEGTLYAGEFAVVGTAG
                     ARPEVVDFLQQFSDAPIQCAQGADPASSRISANINVGPDCYANQILADSAAVLTDAIA
                     NDTGRFDASDLMPGEVGAGSFWTGMVQYMQEGPESLSSVLEEIESSWPSG"
     gene            complement(4794..6161)
                     /locus_tag="VF195_05205"
     CDS             complement(4794..6161)
                     /locus_tag="VF195_05205"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012180593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEX6580251.1"
                     /translation="MSPTPPRFPEGFLWGVATSAFQVEGAVDEDGRGPSIWDTFCRTP
                     SRVRGGDTGDVACDQYHRYPEDVALMDELGIGAYRFSIAWPRVQPDGSGPGNPSGLDH
                     YRRLVEELNGRGIAAVVTLYHWDLPQALEDAGGWPNRDTAERFADFAAIVHRALAGGV
                     AHWITVNEPWVAAWLGYGTGVHAPGRTDDGLALAAGHHLLLAHALARDAIGEPVGITL
                     NLEPHRPASETPPDAEIAHLADLHMNALFLDPLFGRGYPSALMERYRALFDRGFVHEG
                     DLGRIAGPLSFLGVNYYRPHAITTDVDRSDRTTETPGSLGGWDVVPRGTPVTSMGWPV
                     EPSGLLELLQRLHREYSPTRIFLTENGAAFDDGPGPDGVVHDEPRIAFLRDHVAAAGQ
                     AIAAGVPLIGYLVWSLLDNFEWAEGYAKRFGLVHVDFETQVRTPKASARWFQGLVESG
                     SSRPV"
     gene            complement(6158..7393)
                     /gene="ugpC"
                     /locus_tag="VF195_05210"
     CDS             complement(6158..7393)
                     /gene="ugpC"
                     /locus_tag="VF195_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX6580252.1"
                     /translation="MAGIVMEDVSKVFADGTTAVSDLSLDIKDEEFIVLVGPSGCGKT
                     TALRMVAGLESITRGTIAIGDQVVNAVPPKERDIAMVFQNYALYPHMSVYDNMAFGLK
                     LRKLPKEEIDRRVRRAAEILGLEEYLQRKPKALSGGQRQRVAMGRAIVREPQAFLMDE
                     PLSNLDAKLRVQMRAEVSRLQSELGVTTIYVTHDQTEAMTMGDRVAVIKKGELQQVDA
                     PQFLYDHPANLFVAGFIGSPAMNMVEADLVREDGGLYTVFGSTRLRVADEVVSERPGI
                     RAYEGHRVIAGIRPENMEDASIMPVIPEDRRMKVDIVLREALGSEVLVHFSVDAPPVL
                     TEDTRELVGEQSGPMAQASVIVQELEKAAETGTSTFVARLDPRTRAAERQPLELAVDT
                     GRLHFFDPQTGLGIYGDES"
BASE COUNT         1296 a         2676 c         2493 g         1155 t
ORIGIN      
        1 ggccggttcc cgacgagatg gtgcgacgga cgggaggatc gctcccgctg cgctctcatg
       61 gtgctgctga cgctccgagg gacgccgttc ctctactacg gggacgagat cgggatgacc
      121 gacgttgcgg tgcccgagga cgatcagctc gacctggtcg gcgggggcgg agcagagcag
      181 ccgagccgtg acggctgccg gaccccgatg caatggagcg cggatccctc ggccgggttc
      241 acgcgggacg gtgtccgccc gtggcttccg atcggcgacg ccgcgtcgcg gagcgtcgcc
      301 gcgcaacgag aagattccgg ctcgatgctg aacctctgcc gcgacctgat cgcgctccgg
      361 cgcgaggtgc cggacctgcg ttcggggagg tacgtgtcga tcccctcacc cgccggtgtc
      421 tggacctgga gccgcggcga tgacaccgcc gtctcgctga acctgtcgga ggaacccgtc
      481 gcggtcgcga tcggcacggg cacaatcgcg atcggcaccc gtcgtgagcg cgacggccag
      541 cccgtcggtg acgagctcag cctcggtccg tgggagggtg cggtgctgcg cctcgctccc
      601 tcctgatctc gagcaggtga tcggcgacgc cgacgctcgt gaacggcagg acgggcgcga
      661 gcaggagggc cacgtccgtg accgttcgaa ccgtcacgaa cgcccaggca gagacgatca
      721 cgatcgcgag cgcccgtgct cgccaggcgc gatcgagatc ggagcgcatc gccgcgatcc
      781 cccccgcccc cacgccagcg acgaacccgg tgacggcatc ggccgcgacc gcggacaccg
      841 ggatccccac gacgatgcac aggatcatcg ccaagaccgt cgcccggggg gcgcgcgggt
      901 gctgggacag gaacgcgagc gcaaggaaga cgaacggtat gaggcacaac ccgaacgcga
      961 tccagggacc ggcgctcggg gcgcgctcag ggtcgtccga ggcgatcgag acgagcccga
     1021 cgagcagccc ccagtacgcc ggcaccagca ggagcgtcga gagcaggatc gcccgccaca
     1081 cctgacgggg cgacagcggc tgggccccgg cgccggccgg tcgccgagac cgggccatca
     1141 ggagatcagc ccttgaccgc accggccgtg agaccacgca cgaagtactt ctggagcgcg
     1201 aagaacacca acagcggcag cgccatcgag atgaacgccg cggacgtcag caggtgccag
     1261 ttctcgccgc gcgagcccac cagctctcgc agcgcgaccg tgacgacgat attttggccg
     1321 gcgccaccga ggaacacgta ggccaccagc aggtcgttcc acacccacag gaactggaag
     1381 atggcgaacg ccgcgatcgc agggaccgac agcggaacca ccagccgcca gaagatcgtg
     1441 aagtgatccg cgccatcgat ctttgcggac tcgatgatcg aggacggcag cgagccgatg
     1501 tagttccgta acaggtacac ggccagaggc aagccgaacc ccgcatgcgc cagccagatc
     1561 ccgaggaagg tcccctgcag gctgagatcg gggaagatct cgcgaccgaa cagggtcgcc
     1621 ccctgtgtgt acagtcgcag gatggggatc agcgccatct gcagcggcac cacgagcagc
     1681 ccgacgacga tcacgaacag cacgtaccgg ccgcggaact ccatccacga gaacgcgtac
     1741 gcggcgaacg ccgcgatcgt gatcgggatc acggtggagg ggatcgtcac cgccagactg
     1801 ttcaggaagg cgttcccgaa cccgccctgg tccagggcga tccggtagtt ctcgagcgtc
     1861 cactccgtcg ccccgaacgg atgggcgaac acctcccacc acccggaggt gttcaccgca
     1921 tcctccggcc ggaaggacgt gatcagcacg ccgagggtcg ggatcgacca caggatcacg
     1981 ataacgatga tcgtgatgcg gacgaaccag cctccacgac cggccgcctc cagctgatcc
     2041 ccttccggcg cgtgggccgg tgccttcggg gccgcctcga ccgtcgccga gctcaccgga
     2101 tcgcctcctc ttcccggaag cggcggatgt tccacaccat caccgggatc acagcagcga
     2161 acagcaccac agcgatcgcc gcggcgcggc cgttgtggtt gagctggaag aaccagcgga
     2221 tcatccgttc cgcgacgacc accgtcccgt tggtctcggc gctggccggc tggacgaaca
     2281 cgatgtcgaa gaccttcagc gagttgatca ccatgtaggt cgtgaccacg acgatcgtcg
     2341 gcaggatgct cggcaccatg atccgccgga agacctggaa ctccgaggcg ccatcgatcc
     2401 gcgccgcctc gatgatctcg tccgggatcg acttgatcgc agcggagagc actaccatgg
     2461 cgaagccggt ctgtatccag accatgatca ccatcaggaa caggttgttc cagggctgca
     2521 gctccagcca cttcacgggt tcctggccga gcccgatcat gatcccgttc agcaggccga
     2581 tgttcgatcc gaagccctgt gggcggtagc tgtagatcag ctgccacgtg atcgaggcgc
     2641 cggcgaacga gatcgccatc ggcaggaaga tcagtgactt ggcgacggcc tcgccgcgac
     2701 ggagtctgtc cgcgagcgtc gcgtagatca ggccgatggt gacggcggcc aacggtacga
     2761 ggatgatcca cccggccgtg ttccggatcg cgcgcagcat gtcggagtcc gtgaagacga
     2821 acttgaagtt gtccaggccg acgaagctct ggctgttggc gtccttgaag gagatcagca
     2881 ccgtgttgat cacggggtag acgaggaaca ctgcgaggat cacaaggcag gggccgacga
     2941 agacgtacgg tcggacgctc tcgcgcactc gagcggggaa cagatccacc gctcggttca
     3001 tcccccagta caggacgaag acgccgccca cccccacggc gatggccacc ccaaccacga
     3061 tgaaccggtt ggcattttcg tcgcgaagga actcgaacgt ccaccacagg atgtagaacg
     3121 tcgcgacagg gaccgcgacc gcgaccagca cccgcagcag gagcacgagc acgccggtga
     3181 atctccgcga cggaggagtt ccggtcaggc cgggggcgga ggccggttcc gacgtgacgc
     3241 tcacggtcgc atcctccccc ggtcgaagcc accgccgcaa actggaagga gcggccgccc
     3301 tgcggccgcc ccctccgttg caacgagcat cgaccggccc gttcctacct ccccggggtg
     3361 gccgcctatc cggacggcca gctcgactcg atctcctcca acacggacga cagggactcc
     3421 gggccttcct gcatgtactg caccatcccg gtccagaagc tgccggcacc gacctcgccc
     3481 ggcatcaggt ccgaggcgtc gaaccggccg gtgtcgttcg cgatcgcgtc ggtcaggacg
     3541 gcggccgagt cggccaggat ctggttcgcg tagcagtcgg gaccgacgtt gatgttggcc
     3601 gagatcctcg acgacgcggg gtcggcgccc tgggcgcact ggatcggcgc gtccgagaac
     3661 tgctgcagga agtcgacgac ctccggacgg gcaccggcgg tcccgacgac cgcgaattca
     3721 ccggcataca gcgtgccctc cgagtcgatc ggtgggagcg ggaaccagtc ataatcgacg
     3781 cccgcctccg cgtcctcggg gaacggcgcg gccccgatga tgaaggtcgc ctggcggtgg
     3841 agccagcagc ccggcgggtc ctcgaacatc ggcagcggcg ccgagccgaa cgagatcgag
     3901 ggcgtgtcgg ccgctccgcc gagcacgtac ccgtccgtga acatgatgtt cccgaactcc
     3961 tcggccgcgg cggcgaccgc cgggtcggtg aaggggatct cgtgcgtgac ccactggtcg
     4021 taggtgtcca cgccggcggt ccgcagcatg atgtcttcca tccagtccgt ggccggccac
     4081 ccggtcgcgc ctccggactc gaagcccacg caccatggcg taccgccgtc ggccacgatc
     4141 tggtcgctca gcgcgagcat ctcgtcccag gtcgtgggca cctggtaccc ggccgcatcg
     4201 aagtcgtcct tcgggtacca caccatgctt ttcaggttca cgttcgtcgg gatgccgtag
     4261 tgctctccgt ccacctcgac gagcgagaac aggtactcgc cgtaggcctc ctggagcgcg
     4321 cctacatcga tgcccaggtc ctcgagggac acgatggtgc cggcgtccgc ctgctgttgg
     4381 acggtgccgg gctgtggcac gagcgcgatg tcgagcgtgc cgccctcgga gcggatcgcg
     4441 aactgctcct cgaagtcggc cgaggcctcg atctcgacgg cgtagtcggc gttctggagg
     4501 ttctcatcga ccgccgcctg gaccgcctcc ccctcgttcg gctccatggc gttcaggacg
     4561 ttcaccgtgc ccgtgttctc ctccagagca ggaccattac cggtctctcc tccgcctccg
     4621 tcgtcgtcgc tcgtacaggc cgccgcgatc agcaagaaca cggcgaacgg cacgatgagc
     4681 ctgagtggct tcatcgattg ccccctttct cccgccccat tctcccccat gtacgtgatt
     4741 cgaggtcaac tcggcctggc tccgctctca cgctaccctc ccgggttgcc tggtcaaacg
     4801 ggccgagagg aaccgctctc gaccagcccc tggaaccagc gggcactcgc cttcggggtc
     4861 cgcacctggg tctcgaagtc gacgtggacc aggccgaatc gctttgcgta gccctcggcc
     4921 cactcgaagt tgtccagcag cgaccagacg aggtagccga tcagcggcac cccggccgcg
     4981 atcgcctgac ccgcggcggc gacgtggtcg cgcaggaacg cgatccgcgg ctcgtcgtgg
     5041 acgaccccgt ccggtccggg gccatcatcg aacgcggccc cgttctcggt gaggaagatc
     5101 cgcgttggcg agtactcgcg atggagtcgc tgcaacagct ccagcaggcc cgagggctcg
     5161 accggccacc ccatcgaggt gaccggcgtt ccgcgcggca ccacgtccca gccgccgagc
     5221 gatccagggg tctcggtggt ccgatcggac cgatcgacat ccgtcgtgat cgcgtgcggg
     5281 cggtagtagt tcacgccgag gaacgacagg ggacctgcga tgcggcccag gtcgccctcg
     5341 tgcacgaacc cgcggtcgaa cagagcccgg tagcgttcca tgagcgccga ggggtatccc
     5401 cgcccgaaca gcggatccag gaacagggcg ttcatgtgca ggtccgccag gtgggcgatc
     5461 tccgcgtccg gcggagtctc ggacgccgga cgatggggct ccaggttcag cgtgatcccg
     5521 accggttcgc cgatggcgtc ccgagccagg gcgtgggcga gcagcaggtg atggccggcg
     5581 gcgagcgcga ggccgtcatc ggtgcgaccg ggcgcgtgga ccccggtgcc gtatcccagc
     5641 caggccgcga cccacggctc gttcaccgtg atccaatgcg ccacgcctcc tgcgagcgct
     5701 cggtgcacga tggcggcgaa gtctgcgaac cgctcggcgg tgtcgcggtt cggccaccca
     5761 cccgcatcct cgagtgcctg gggaaggtcc cagtggtaca aggtgacgac ggcggcgatg
     5821 ccgcgaccgt tcagctcctc gacgagcctg cggtaatgat cgagcccact ggggttcccg
     5881 ggtccgctgc cgtccggctg caccctcggc cacgcgatgg agaaccgata ggcgccaatc
     5941 ccgagctcgt ccatgagcgc gacgtcctcg gggtaccggt ggtactggtc gcacgccacg
     6001 tctcccgtat ccccgccacg caccctcgac ggtgtcctgc agaacgtgtc ccagatcgaa
     6061 ggtcctcgcc cgtcctcgtc gacggcgccc tcgacctgga acgcggaggt cgcgaccccc
     6121 cacaggaacc cctcggggaa tcgggggggc gtgggactca gctctcgtcc ccgtagatac
     6181 cgagccccgt ctgcggatcg aagaaatgca ggcgaccggt gtcgaccgcg agctcgagcg
     6241 gctggcgttc ggccgcgcgc gttcgcggat cgagccgagc gacgaacgtc gacgttccgg
     6301 tctcggccgc cttctccagc tcctggacga taacgctcgc ttgggccatg gggccgctct
     6361 gctcgcccac gagctcacgc gtatcctcgg tcaggaccgg gggcgcatcc accgagaagt
     6421 gcaccaacac ctcggaccca agcgcctcac gcaggacgat gtcgaccttc atccgccggt
     6481 cctccgggat gaccggcatg atggaggcgt cctccatgtt ctccgggcgg atccccgcga
     6541 tcacacggtg cccctcgtag gcgcggatcc cggggcgttc cgagaccacc tcatcggcca
     6601 cgcgcagcct cgtgctgccg aacaccgtgt agagcccacc gtcctcgcgc acgagatcgg
     6661 cctcgaccat gttcatcgct ggtgacccga tgaagcccgc cacgaacagg ttggccgggt
     6721 ggtcgtacag gaactgtgga gcgtcgacct gctgcagctc gcccttcttg atgaccgcca
     6781 cgcggtcccc catcgtcatc gcctcggtct gatcgtgggt cacgtagatc gtggtgacgc
     6841 cgagttcgct ctgcaggcgt gagacctcgg cccgcatctg gacgcgcagc ttcgcgtcga
     6901 ggttggacag cggctcgtcc atcaggaaag cctgtggctc gcggacgatc gcacgaccca
     6961 tcgcgacccg ttgacgttgc ccacccgaca gcgcctttgg cttgcgctgg aggtactcct
     7021 ccagcccgag gatctcggcg gcccgccgaa cgcggcgatc gatctcctcc ttgggcagct
     7081 tgcgcagctt gagcccgaac gccatgttgt cgtagacgct catgtgcggg tacagcgcgt
     7141 agttctggaa gaccatcgcg atgtcccgct ccttcggagg cacggcgttg accacctgat
     7201 cgccgatcgc gatcgttccg cgggtgatgc tctccagccc ggcaaccatc cgcagcgcgg
     7261 tcgtcttgcc gcacccggac ggtccgacca gcacgatgaa ctcttcgtcc ttgatgtcga
     7321 gcgagaggtc cgagacggcg gtcgtgccgt ctgcgaacac cttggagaca tcctccatca
     7381 cgatcccggc catcccgctc ctcctgttcc ctggccgtgt tgccggcgcg gcgactagaa
     7441 accattcaac ctcaaccggc ttgcctgggt cgagtccgtc ccgccctcag ccgctcgaga
     7501 cggcgggcag cacctcgcgc tcccggcgtc gcgacacctc tcgcccgaac tcgcgttccc
     7561 gttcgtcccg agccctgcga tagccctcga gcgcggcctc gatcgagccc ggcgccgcgt
//
LOCUS       DASUYD010000090         3841 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1590567, whole genome shotgun sequence.
ACCESSION   DASUYD010000090 DASUYD010000000
VERSION     DASUYD010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1590567"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VF195_05215"
     CDS             <1..1072
                     /locus_tag="VF195_05215"
                     /inference="COORDINATES: protein motif:HMM:NF024554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX6580253.1"
                     /translation="TAETPIYKKGEILYNLHKARQAVARSGQVFVVEGYTDVIGLAQA
                     GIDNAVATCGTALGEGHFRLLSRFAQQAVLAFDSDEAGARAAERAFGYQEQYPVQAVV
                     MIMPEGLDPAEFVAKHGPDEVREAAKSARPLVEYMVRRTVERHDLSNVEGQSAAVASA
                     LPILQELTDPVRRSEYGHLLADLTGVSEDSVRTALDRRLGGHPEQVAKTLKRLSARDR
                     VEREMLRLLVRDRAIFEEFAPQLTEDHVRTPGGRAVLTGLREHAGDVSALAGSEDPKI
                     ASAVSALAVEPIEGEPTREYASHVLARLQEFVLKAKSDELRIRLQKLNPQTEAGYDEL
                     FHELVAVDGDLRRIRQGLLDAG"
     gene            1154..2470
                     /gene="rpoD"
                     /locus_tag="VF195_05220"
     CDS             1154..2470
                     /gene="rpoD"
                     /locus_tag="VF195_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006721486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX6580254.1"
                     /translation="MSAAREPTTDTQSLDEVKEQLVVLGRERGFVTSGDVFEALPEIT
                     PDQVEEVLAQLTDHLNTEGIEIIELPGDDTDAVTQIRIEVDALKAPTNDPVRMYLKEI
                     GKVPLLNAPQEVDLARRIEAGELCTELQAQISMEGKPDPKRFRLATTRVWEIWDHQVR
                     AFGKVEGIGRDPLAQKKSYRPKTDHDLTSYLHRVERDGQLAKRKLIEANLRLVVSIAK
                     RYVGRGMLFLDLIQEGNLGLIRAVEKFDHSKGFKFSTYATWWIRQAITRAIADQARTI
                     RIPVHMVETINKLVRVQRQLLQDLGREPTPEEIGKVMGIGAEKVREIQKVSQEPVSLE
                     TPIGEEEDSHLGDFIEDSDAVVPVDAASFILLQEQLGSVLHTLSEREKKVIELRFGLA
                     DGHPRTLEEVGREFGVTRERIRQIESKTLSKLRHPSRSQRLRDYLE"
     gene            complement(2489..2665)
                     /locus_tag="VF195_05225"
     CDS             complement(2489..2665)
                     /locus_tag="VF195_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6580255.1"
                     /translation="MGLYAAMKERMASEEGATAVEYALMIALIALVIVVAVTFLGQET
                     NSVYENGELITALT"
     gene            2831..3280
                     /locus_tag="VF195_05230"
     CDS             2831..3280
                     /locus_tag="VF195_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580256.1"
                     /translation="MGSVLCTVKAAHPEADVRGEEERTRALEGMVSLITKVGAIAPLE
                     PGERVLCAAYVSGTRSSIGAALIAVSDRRLLHAPRSAFHSRGAWRAKRLDDIRVESRT
                     RRSLGLAAAHQFEIIGVDGSGMAFELENRRDARAFNDALGRRLVERG"
     gene            complement(3322..3498)
                     /locus_tag="VF195_05235"
     CDS             complement(3322..3498)
                     /locus_tag="VF195_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017233461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEX6580257.1"
                     /translation="MELIARVQQRLDPEDGATAVEYALMVALIAMVIIASVVFLGQVT
                     NQTFQDVGSAVSSA"
BASE COUNT          656 a         1272 c         1329 g          584 t
ORIGIN      
        1 caccgcggag acgcccatct acaagaaggg cgagatcctc tacaacctcc acaaggcccg
       61 gcaggcggtg gcgaggtccg ggcaggtgtt cgtcgtcgag ggctacacgg acgtgatcgg
      121 gctcgcgcag gcgggcatcg acaatgcggt cgccacgtgc ggcacggctc tgggagaggg
      181 gcacttccgg ttgctctcac ggttcgccca gcaggcggtc ttggcgttcg actccgacga
      241 ggccggggcg cgcgccgcgg agcgggcgtt cggctaccag gagcagtatc ccgtccaagc
      301 cgtcgtgatg atcatgccgg agggcctgga cccggctgag ttcgtcgcga agcacggccc
      361 ggacgaggtc agggaggcgg ccaagagcgc tcgcccgctg gtggagtaca tggtcaggcg
      421 gaccgtggag cgtcacgacc tctccaacgt cgagggacag tccgcggcgg tcgcgagcgc
      481 cctgccgatc ctccaggagc tgacggatcc cgtccgtagg agcgagtacg ggcatctgct
      541 ggcggacctc accggtgtct cggaggactc cgtgaggacg gcgctggatc ggcggctggg
      601 cggtcatccc gagcaggtcg cgaagacgct gaagcggctg tccgctcggg accgcgtgga
      661 gcgggagatg ctgcgcctct tggtccgcga tcgcgcgatc ttcgaggagt tcgcgccgca
      721 gctcaccgag gatcacgtgc ggacgccggg ggggcgagcg gtgctcacgg gtcttcgtga
      781 gcacgccggc gatgtgagtg cgctggccgg atccgaggac ccgaagatcg catcggcggt
      841 gagcgcgctg gcggtggagc cgatcgaggg cgagcccacg cgcgagtacg cgtcgcacgt
      901 cctcgcgcga ctgcaggagt tcgtgttgaa ggcgaagagc gacgaattgc gcatccggct
      961 gcagaagctg aatccgcaga cggaagctgg ctacgacgag ctcttccacg agctggtggc
     1021 ggtcgacggt gatctccgca ggatccgaca gggactgctc gacgccggat agcgcgccgc
     1081 tcggtccggg tcggcgccgc cgcgggttcc gtacactgtc ctccgtgctc ccgacgcagc
     1141 tggaagaccc ccgttgagcg cggcccgcga accaacgact gatacgcaga gcctcgacga
     1201 ggtcaaggaa cagctcgtcg tgctcggccg cgaacgcggc ttcgtcacct caggcgacgt
     1261 cttcgaggcc ctccccgaga tcacacccga tcaggtcgag gaggtcctcg cgcagctgac
     1321 ggaccacctc aacaccgagg gcatcgagat catcgagctc cccggtgacg acacggacgc
     1381 ggtgacgcag atccggatcg aggtggatgc gctcaaggcg cccacgaacg atccggtccg
     1441 catgtacctg aaggagatcg ggaaggtccc cctgctgaac gcgccgcagg aggtcgacct
     1501 ggcccgcagg atcgaggccg gggagctctg cacggagctc caggcccaga tctcgatgga
     1561 gggcaagccc gaccccaagc gcttccggct cgccaccacg cgggtctggg agatctggga
     1621 ccaccaggtt cgggcgttcg gcaaggtgga gggcatcggc cgggacccgc tcgcccagaa
     1681 gaagtcctac cggcccaaga cggaccacga tctcacctcc tacctccacc gggtggagcg
     1741 ggacggccag ctcgcgaagc ggaagctgat cgaggcgaac ctgcgcctcg tggtctcgat
     1801 cgcgaagcgc tatgtgggcc gggggatgtt gttcctcgac ctgatccagg aggggaacct
     1861 gggcctgatc cgggccgtgg agaagttcga ccactcgaag ggcttcaagt tctcgacgta
     1921 tgccacgtgg tggatccgcc aagcgatcac gcgcgcgatc gcggaccagg cgcggacgat
     1981 caggatcccc gtgcatatgg tcgagacgat caacaagctc gtccgggtgc agcggcagct
     2041 gctgcaggac ctcggccgcg aaccgacgcc cgaggagatc gggaaggtca tggggatcgg
     2101 cgcggagaag gtccgcgaga tccagaaggt gtcccaggag cccgtgtccc tggagacgcc
     2161 gatcggcgag gaggaggact cccacctcgg cgatttcatc gaggactccg acgccgtggt
     2221 ccccgtggac gcggcgtcgt tcatcctgct gcaggagcag ctcggctcgg tcctccacac
     2281 gctgtcggag cgcgagaaga aggtgatcga gctccggttc ggtctggccg acggccatcc
     2341 ccgcacgctc gaagaggtcg gccgggagtt cggtgtcaca cgggagcgga tccgccagat
     2401 cgagtcgaag acgctttcga agctccggca cccctcacga tcgcagcgcc tgcgcgacta
     2461 cctcgagtag cccggcatgc ggtagcgatc aggtgagggc ggtgatcagc tcaccgttct
     2521 cgtacacgga gttcgtttcc tggccgagga acgtcacggc caccacgatc acgagagcga
     2581 tcaatgcgat catgagggcg tactcgaccg cggtggctcc ctcctccgaa gccatccgct
     2641 ccttcatcgc agcgtagagt cccatcctcc caacgcctgc ctttctcaac cgagcgccga
     2701 gacgccctgg tgtactcgtg aacccgaaca ccaagttgcc caacgatccc ggcagcccct
     2761 ccgccggccg gtcgagcaac gcacttgtac cacaacgaca tcggcatgta tatcggagag
     2821 cttgagccgg ttgggctccg tactctgcac cgtcaaggcg gcccatcccg aggccgatgt
     2881 ccggggtgag gaggagcgca cgcgcgcgct cgaagggatg gtttctctca tcaccaaggt
     2941 cggagcgatc gcgccgctcg aaccggggga gcgggtcctg tgcgccgcct atgtctcggg
     3001 tacgaggtcc tcgatcgggg cggcgctgat cgccgtgtcg gatcgccggc tcctccacgc
     3061 accccgctcc gccttccact cacgcggagc gtggcgcgcg aagcgactgg atgacatccg
     3121 cgtcgagtcg aggactcgga ggtccctggg actcgcggcc gcgcaccagt tcgagatcat
     3181 cggtgtcgac ggctcgggca tggcgttcga gctggagaac cggagggacg cgagggcgtt
     3241 caacgacgcg ctcgggcgac gtctggtcga acgaggctga ccccgacgag gtcgagatcc
     3301 ctcgcgtcct cgatacccga ttcaggccga tgaaacggcc gaaccgacgt cctggaacgt
     3361 ctggttcgtc acctgcccga ggaatacgac cgacgcgatg atcaccatcg cgatcagggc
     3421 caccatcaac gcgtactcga ccgcggtggc accgtcctca ggatccagcc gctgctgcac
     3481 ccgtgcgatg agctccatcc ctcatgcacc ttcccgtcct cagggcccgt tggcccgagc
     3541 gccgggccac ccatcccctg gggaagatcc tcgtcgctcg atgacagcat cggccgcgag
     3601 gcctctcccg ctggagtcag cccactgggg ggttgctgcg acctacaggg gcgccgccgg
     3661 atgccgtggg tcagttccag gcggcggcgt cgtcctcgcc gagacgctcg cggatctcgc
     3721 cgcggagccg ccggatcgca tcgaggctct tgcccgtggc tgcgtcggtg agccgctgga
     3781 cctgggtcga gacgagccgc agagccggac gctgctcgct cctccggcgc tgtgggccat
     3841 g
//
LOCUS       DASUYD010000091         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_268103, whole genome shotgun sequence.
ACCESSION   DASUYD010000091 DASUYD010000000
VERSION     DASUYD010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268103"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1305
                     /locus_tag="VF195_05240"
     CDS             <1..1305
                     /locus_tag="VF195_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX6580258.1"
                     /translation="LDSDHARAEGEVGKTGVAIDSIEDMERLLAGIPLDQVSTSMTIN
                     ATAPILLLLYELVAEEQGGLDPATLSGTVQNDLLKEYAARGTYIYPPEPSMRLITDLF
                     AYAGERIPRWNTISISGYHMREAGATAAHEIAFTLANGIAYVKAAVDAGLAVDDVAPR
                     LSFFFACHMHFFEEIAKFRAARRMWAAIMRDRFGAKDPRSLTLRFHTQTGGATLTAQQ
                     PENNIVRTALEAMAAVLGGTQSLHTNSFDEALALPTEQAVKIALRTQQVLGFETGVTE
                     TVDPLGGSYHVEALTDELERIATEIVETIDAMGGAVPAIEAGYYQDEIHEAAFRIQRG
                     IETGERIVVGVNRFVDADARAVELQRISVEETAQQMERLRELRASRDQPAVDAALLEV
                     EETARGTGNLLPPMREALRLRSTLGEVSDALRRVFGEYRPPY"
     gene            1330..2022
                     /gene="npdG"
                     /locus_tag="VF195_05245"
     CDS             1330..2022
                     /gene="npdG"
                     /locus_tag="VF195_05245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX6580259.1"
                     /translation="MDVAVIGGTGAEGLGVAMRAAAAGHHVTIGSRNAGRGAEAAAKA
                     AGRAGAALEGTDNASAAAGAGGDGVVVITVPYEGQAEIYRSIRDAVAPGAVILDATSP
                     LATAVGGRPWQVLRPWHGSAAEQAQAILGQGPRVVAGLHTIAAEALQALDHEVDSDVL
                     LCADDPEAKQRVGSLIDGIPGMRWVDCGRLEMARVAEPLTALLVSINRTYKLKDSGFR
                     IVGRDAWGGASP"
     gene            complement(2019..3740)
                     /locus_tag="VF195_05250"
     CDS             complement(2019..3740)
                     /locus_tag="VF195_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX6580260.1"
                     /translation="MARKQKLLIANRGEIAIRIARTCRDLGISTVAVYSDADRDALHV
                     EIADESFRIGPGLASSSYLAIDRVMEVAVKSKATLVHPGYGFLAERATFAEAVEDAGL
                     AFVGPPSQVIATMGDKAAARRVADAAGMPIVPGTREPVDLAQAKKQAARIGFPLMVKA
                     AFGGGGKGMHVVRDADHLEESLKRAAREARSYFGRPEVFLERYVDRAHHVEAQIVADT
                     HGNVFFLGERDCSVQRRHQKLIEETPSPLFDEKIRERFAEATTTLAREAGYVNAGTVE
                     CVMDEDGDFYFLEMNTRLQVEHTVTEMVTGLDLVALQIRVALGEELALDPAPRGASIQ
                     CRINAEDPGRNFLPGPGRVTSYREPAGPFVRVDSGIEQGREIPGDYDSMFAKLIVSGH
                     DRDTARVRMLRALDEFRVEGVPTTIPVHRWILESKEFRAGRHTTTWLERALADAALPA
                     HPDLAPSGAPPNRPADILVEVDGRRVPVRIFDERREPAPRPPATPDAHHGEHVHGEIL
                     APMQGTIVKVLVEKGQEVRPGDVICILEAMKMENHVPSTRDGEVTDLPIHAGQVVETG
                     QVLAVID"
     gene            3780..4529
                     /locus_tag="VF195_05255"
     CDS             3780..4529
                     /locus_tag="VF195_05255"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEX6580261.1"
                     /translation="MSEEALERALAAAGLSAPVRWDDVTESTNATALVLAAEGAPSWT
                     LVAASHQTAGRGRHGRIWRDRSGSALLCSLVLRLDWPPDRVGLVSLAAGAAMAEAISE
                     IAGVEIRCKWPNDLMAGDAKIGGILGEAQVSAEAVAHVVVGIGVNLDVPEGIPRAGAI
                     GSLDEEALLESFLRRYSSMLGASADEIVPRWRAVSRTLGRRVEATTVDGDVARGLALD
                     IDDTGALLIDTDVGPARVAFGEVEHLDVAPG"
     gene            4577..5188
                     /locus_tag="VF195_05260"
     CDS             4577..5188
                     /locus_tag="VF195_05260"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX6580262.1"
                     /translation="MAFPTRLLIEGEELVMDLRPHWIALIMPTIATLATFAVMLVLYD
                     AFDEDILDTVVGIAGSLFLLAYPIRKLVDWLTSHFVVTSDRIIHRRGFIAKYSMEVPL
                     EAINDVRFEQGVVDRIIGAGTLVVQSASEAGRQVFDHIRKPEEVQKTIYHQGELNQQR
                     MMRGSEGRSSHSSTSELERLADLRARGVLTEEEFQAQKRRLLG"
     gene            5190..5585
                     /locus_tag="VF195_05265"
     CDS             5190..5585
                     /locus_tag="VF195_05265"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580263.1"
                     /translation="MGPEGPIVAIDHVYYWTRDMDRAVAFYENILGLPLSRRTGNEWA
                     EFHSEGIRLALHGTEEASTPPSGTVVFRVEDLDEARWALQQRGVEFDGHESEVPGVAR
                     FTTFHDPDGNLLQLIEYGSPGASPSSSAS"
BASE COUNT          862 a         1908 c         1956 g          859 t
ORIGIN      
        1 ctcgactccg atcatgcccg cgccgagggc gaggtcggga agacgggcgt ggcgatcgat
       61 tcgatcgagg atatggagag gctcctcgcg gggatcccgc tggatcaggt gtccacctcg
      121 atgacgatca acgccaccgc cccgatcctc ctcctcctct acgagctcgt ggcggaggag
      181 cagggcggcc tcgaccccgc gaccctctcc ggcaccgttc agaacgacct gctgaaggaa
      241 tacgcggcac gcgggacgta catctatccg cccgaaccgt cgatgcgcct gatcacggat
      301 ctgttcgcct acgcggggga gcggatcccg cgctggaaca cgatctcgat aagcgggtat
      361 cacatgcgcg aagccggcgc gacggccgcg cacgagatcg cgttcacgct ggcgaacggc
      421 atcgcatacg tgaaggcggc cgtcgatgcc ggcctcgccg ttgacgacgt ggcgccgcgc
      481 ctctcgttct tcttcgcctg ccacatgcac ttcttcgagg agatcgcgaa gttccgcgcc
      541 gcgcggcgga tgtgggccgc gatcatgcgg gatcggttcg gcgccaagga cccacggtcg
      601 ctgaccctcc gcttccacac gcagaccggt ggcgccacgc tcaccgcgca gcagccggag
      661 aacaacatcg tccgcacggc gctggaagcg atggcggcgg tgctgggcgg gacccaatcg
      721 ctccacacca actcgttcga cgaggccctt gcgctcccca cggagcaggc ggtgaagatc
      781 gcgctccgaa cccaacaggt cctcggcttc gagacgggcg tgaccgagac ggtcgacccg
      841 ctcggcggct cgtaccacgt ggaagcgctc accgacgagc tggaacggat cgcgaccgag
      901 atcgtggaga cgatcgacgc gatggggggc gccgtgcctg cgatcgaggc gggctactac
      961 caggacgaga tccacgaggc ggcgttccgg atccaacgag ggatcgaaac gggcgagcgg
     1021 atcgtggtgg gagtgaaccg attcgtcgac gcggacgcga gggccgtgga gctccagcgg
     1081 atctccgtcg aagagaccgc acagcagatg gaacgactcc gcgagctccg cgcctcccgg
     1141 gatcagccgg ccgtcgatgc cgcgctcctg gaggtggagg agaccgctcg cgggacgggc
     1201 aacctcctcc cgccgatgcg cgaggcgctc cgtctgcgat cgacgttggg cgaggtgtcc
     1261 gatgcactcc gtcgcgtgtt cggggagtat cgccctccct actgatcttg gcctactagg
     1321 ctgcgcatcg tggacgtggc ggtgatcggt ggtacgggcg ccgagggact cggtgtggcc
     1381 atgcgcgcgg ccgcggcagg ccaccacgtg acgatcgggt cccggaacgc ggggcgcggt
     1441 gcggaggccg cggcgaaggc cgccgggcgc gcaggcgccg cgttggaagg caccgacaac
     1501 gcctccgcgg cggccggcgc cgggggcgac ggcgtcgtgg tgatcaccgt cccgtacgaa
     1561 ggtcaggccg agatctaccg ctcgatcaga gacgccgtcg cccccggcgc agtgatcctc
     1621 gatgccacca gccccctcgc cacggcggtg ggcgggcggc cctggcaggt gctccgcccc
     1681 tggcacgggt cggccgccga gcaggcgcag gcgatcctcg gtcaggggcc gcgcgtcgtc
     1741 gcagggctcc acacgatcgc ggcggaggcc ctccaggctc tcgaccacga ggtcgacagc
     1801 gacgtcctct tgtgcgcgga cgatccggag gcgaaacagc gcgtaggctc gctgatcgac
     1861 ggcatccccg gcatgcgttg ggtggattgc ggccgcctcg agatggcccg ggtcgccgag
     1921 cccctcacgg cgctcctcgt gtcgatcaac cgcacctaca agctgaagga ctccggcttc
     1981 cggatcgtcg gtcgcgacgc ctggggcggc gcgtcgccct aatcgatcac cgcgaggacc
     2041 tgcccggtct ccacgacctg gccggcgtgg atcgggaggt cggtgacctc gccgtcccgg
     2101 gtcgacggga cgtgattctc catcttcatc gcctcgagga tgcagatcac gtcgcccggg
     2161 cggacctcct ggcccttctc gacaaggacc ttcacgatcg tgccctgcat cggggcgagg
     2221 atctcgccgt gtacgtgctc gccgtggtgc gcgtccgggg tcgccggggg tcgcggggcg
     2281 ggctccctcc gctcgtcgaa gatccggacc gggacacggc gtccatccac ctcgaccagg
     2341 atgtccgcag gccgattcgg gggcgcgccc gacggcgcga gatcggggtg ggccgggagc
     2401 gccgcatcgg cgagggcgcg ttccagccac gtggtcgtgt gcctgccggc tcggaactcc
     2461 ttcgactcca ggatccaccg gtgcaccggg atcgtcgtcg gcacgccctc cacccggaac
     2521 tcgtcgagcg cccggagcat ccgcacccga gccgtgtcgc ggtcgtgtcc ggacacgatc
     2581 agcttggcga acatcgagtc gtagtcgccg gggatctccc ggccttgctc gatgccggaa
     2641 tcgacccgca cgaacgggcc cgctggttcc cggtagctgg tgacccggcc cggcccgggc
     2701 aggaagttcc tgccggggtc ctcggcgttg atgcggcact ggatcgaggc cccgcgcgga
     2761 gccgggtcga gcgcaagctc ctcgccgagc gccacacgga tctggagagc taccagatcg
     2821 aggcccgtca ccatctccgt caccgtgtgc tcgacctgga gccgggtgtt catctccagg
     2881 aagtagaaat ccccgtcctc gtccatcacg cactcgaccg tgccggcgtt cacgtagccg
     2941 gcctccctgg ccagggtcgt cgtcgcctca gcgaatcgct cacggatctt ctcgtcgaac
     3001 agcggtgacg gcgtctcctc gatcagcttc tggtggcggc gctgcacgga gcagtcgcgt
     3061 tcgcccagga agaagacgtt gccgtgggta tcggcgacga tctgggcttc gacgtggtgg
     3121 gcccggtcga cgtagcgctc gaggaacacc tcgggccgtc cgaagtacga cctcgcctca
     3181 cgcgccgcgc gcttgaggga ctcctccaga tggtcggcgt cgcgcacgac gtgcatgccc
     3241 ttgccgcctc cgccgaacgc cgccttcacc atgagtggga acccgatccg ggcggcctgc
     3301 ttcttcgcct gcgcgaggtc caccggttcg cgcgtccccg ggacgatggg catccccgct
     3361 gcgtcggcga cccgccgggc ggcggccttg tcgcccatcg tcgcgatcac ctgcgaaggc
     3421 gggcccacga acgcgagccc tgcgtcctcg acggcctccg cgaacgtcgc ccgttccgcg
     3481 aggaagccgt acccgggatg aacgagcgtc gccttcgact tcacggcgac ctccatgacc
     3541 cggtcgatcg cgagatagga cgaggatgcc agccccggtc cgatcctgaa ggactcgtcg
     3601 gcgatctcga cgtggagggc gtcacggtcg gcgtcggaat agacggcaac cgtggagatg
     3661 ccgaggtcgc gacacgtccg agcgatccgg atggcgatct cgccgcggtt ggcgatcagg
     3721 agcttctgct tccgcgccat gtggcgccac gatatagcgt cgaggtcggt ggacacgtgt
     3781 tgagcgaaga ggccctcgaa cgggcgctcg ccgcggcggg tctgtcggcg cccgtccgct
     3841 gggacgacgt gaccgagtcc acgaacgcga cagcgcttgt gctcgcggcc gagggggcgc
     3901 cgtcctggac gctcgtggcc gcctcgcatc aaacggcagg tcggggccgg cacggacgca
     3961 tctggcggga ccgatccggc tcggcgctgc tgtgcagcct cgtgctcaga ctcgactggc
     4021 cgccggaccg ggtcggactc gtgtcgctcg cggcgggggc cgcgatggcg gaagcgatct
     4081 cggagatcgc gggcgtggag atccgttgca agtggcccaa cgacctcatg gctggcgacg
     4141 cgaagatcgg cgggatcctg ggtgaagcgc aggtctccgc cgaagccgtc gctcacgtcg
     4201 tcgtggggat cggcgtgaac ctcgacgtgc ccgaaggcat cccgagggct ggggcgatcg
     4261 ggagcctgga cgaggaggcc ctcctcgaga gcttcctccg tcgatactcg tcgatgctcg
     4321 gggcgtccgc cgacgagatc gtgccccggt ggcgggccgt ttcccggacc ctgggccgcc
     4381 gcgtcgaggc gaccaccgtc gacggggatg tcgctcgcgg gctggcgctc gacatcgacg
     4441 acacgggcgc gctgctcatc gacacggacg tcggtcccgc gcgagttgcc ttcggggagg
     4501 tcgagcacct cgacgtcgca ccaggctgac ggatgcccgc ttgcgaggcc ggcgcccctc
     4561 gcgctaggtt ccgcgcatgg cgttcccgac acggttgctg atcgagggcg aggaactggt
     4621 catggacctc cgcccgcatt ggatcgcgct gatcatgccc acgatcgcga cgctcgcgac
     4681 cttcgcggtc atgctcgtcc tctacgacgc gttcgacgag gacatcctcg ataccgtggt
     4741 ggggatcgcg gggagcctgt tcctgctcgc gtacccgatc cgcaagctgg tggactggct
     4801 gacatcgcat ttcgtggtca cgagcgaccg gatcatccat cggcggggct tcatcgcgaa
     4861 gtattcgatg gaggtgccgc tggaggcgat caacgacgta cggttcgagc aaggcgtcgt
     4921 cgaccgcatc atcggggccg gaacgctcgt ggtgcagtcg gcgtccgagg cgggccgtca
     4981 ggtgttcgat cacatccgca aacccgaaga ggtccagaag acgatctatc accagggaga
     5041 gctgaaccag cagcgcatga tgcgtggcag cgagggccgg tcatcgcact cgtccacgtc
     5101 ggagctcgag cgcctcgcag acctccgagc gcgcggcgtc ctcacggagg aggagttcca
     5161 ggcacagaag cgcaggctcc tcggctgaca tggggccgga agggccgatc gtggcgatcg
     5221 accacgtcta ctactggacc cgcgacatgg accgggcggt ggccttctac gagaacatcc
     5281 tcggtcttcc cctgtcgcgg cggaccggca acgaatgggc ggagttccat tcagagggca
     5341 tccgcctcgc cctccacggc accgaggagg cctccacgcc gccgagcggc accgtcgtct
     5401 tccgggtcga ggacctcgac gaggccagat gggctctcca gcaacgaggc gtcgagttcg
     5461 acggacatga gtccgaggtt cccggagtcg cgaggttcac gacgttccac gaccccgacg
     5521 ggaatctcct gcagctgatc gagtacgggt ccccgggagc atctccgagc tcaagcgcca
     5581 gctga
//
LOCUS       DASUYD010000092         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_930942, whole genome shotgun sequence.
ACCESSION   DASUYD010000092 DASUYD010000000
VERSION     DASUYD010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_930942"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF195_05270"
     CDS             <1..684
                     /locus_tag="VF195_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX6580264.1"
                     /translation="LRNTRHVVVQSLELGSLEAFADAFLPAAAWAEREGNVTDWEGRS
                     QPIHPVRGPAGVSRPDWQIFAGLAAAMGRPLGFESLEALRAEAAALLEPRTVAARATA
                     WTGTGPPKRLGELTLLTYPLLVDEGRLSEGADELKAALGEDAFVEVHPEDAEKRGLFD
                     GARAMVRTDAGEAMLPVRVSDRIAQGALFVPFNQPGLEANRLLAGSFTTSAEIVVVAK
                     AAERAEGAA"
     gene            684..1805
                     /gene="nuoH"
                     /locus_tag="VF195_05275"
     CDS             684..1805
                     /gene="nuoH"
                     /locus_tag="VF195_05275"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6580265.1"
                     /translation="MDWLDWLLLIARVAVVFFALLILVMLVIWMERKVIADMQTRLGP
                     MRAGPRGVLITLADGIKLFFKEGITPTLVDRPVYLVAPVIAMLPAFLSFAVIPFGTGV
                     VLFGREVPFQIADLSIGILWILAMSSLMVYAIVLAGWSSGSNYPLLGSVRSSAQMISY
                     EVGMALAIVAVVMYSDALRMSEIVASQDRIWNIIPQFPAFVIYLIAGLAETNRPPFDL
                     PEAESELVAGYHTEYSGIKFAMFYLGEYLNTVTVAAVATTLWLGGWRGPAPDVLPWLW
                     PLLWFLLKVLVIVYLYIWIRATVPRIRYDRLMAFGWKRLIPFGLLWVMVTGAIVVLPD
                     EFGRDTVLTALAIGFGALFVISLVWPLIAPRASEEVPVP"
     gene            1871..>2077
                     /locus_tag="VF195_05280"
     CDS             1871..>2077
                     /locus_tag="VF195_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006371693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6580266.1"
                     /translation="MGTVFRQIFRHDVTEEYPKEIEPPPPRSHIGRHLLNRHENGLEK
                     CIGCELCAFACPADAIWVEGEDNDP"
BASE COUNT          280 a          679 c          742 g          376 t
ORIGIN      
        1 ctgcggaaca cgcggcacgt ggtcgtccag tcgctcgagc tcggctcgct cgaggcgttc
       61 gccgacgcct tcctgcccgc cgccgcctgg gccgaacgcg agggcaacgt caccgactgg
      121 gaggggcggt cgcaaccgat ccatcccgtc cgcgggccgg ccggcgtgtc gcgcccggac
      181 tggcagatct tcgccggtct cgccgcggcg atgggacgcc cgctcgggtt cgagtcgctg
      241 gaggcgctgc gggcggaggc ggccgccttg ctcgagccgc gaaccgtggc ggctcgggcg
      301 accgcttgga cagggaccgg acccccgaag cggctgggag agctgaccct gctcacgtac
      361 ccgctgctgg tcgacgaggg gcgactgtcg gagggcgcgg acgagttgaa ggcggcactc
      421 ggcgaggacg cgttcgtcga agtgcatccc gaggacgccg agaagcgcgg gctcttcgat
      481 ggtgcccgtg cgatggtgcg caccgatgcg ggagaggcca tgcttcccgt tcgcgtctcc
      541 gaccggatcg cgcagggcgc gctgttcgtt ccgttcaatc agcccgggct cgaggccaac
      601 cggctgctcg cggggtcgtt cacgacatcg gcggagatcg tcgtcgttgc caaggcggct
      661 gagcgggccg agggggccgc ctgatggatt ggctcgactg gctgctgctg atcgcgcgcg
      721 ttgcggtcgt gttcttcgcc ctgctgatcc tcgtgatgtt ggtgatctgg atggagcgca
      781 aggtgatcgc cgacatgcag acgcgcctcg ggccgatgcg ggccgggccg cggggtgtgc
      841 tcatcacgct cgccgacggc atcaagctct tcttcaagga gggcatcacc ccgacgctgg
      901 tggaccggcc cgtctacctc gtcgcgccgg tgatcgcgat gctgccggcg ttcctctcct
      961 tcgcggtcat cccgttcggg acgggcgtgg tcctgttcgg acgcgaggtt ccgttccaga
     1021 tcgccgacct gtcgatcggc atcctgtgga tcctcgcgat gagctcgctg atggtctacg
     1081 cgatcgtgct ggccggctgg tcgagcggct ccaactaccc gctgctgggg agcgtccgga
     1141 gctcggcgca gatgatcagc tacgaggtgg gcatggccct cgcgatcgtc gcggtcgtca
     1201 tgtattcgga tgctctgcgc atgtcggaga tcgttgcgag ccaggaccgg atctggaaca
     1261 tcatcccgca gttccccgcg ttcgtgatct acctgatcgc cgggctcgcc gagaccaatc
     1321 ggccgccgtt cgacctgcct gaggcggaat ccgagcttgt cgccggctac cacaccgagt
     1381 actcgggcat caagttcgcg atgttctacc tcggtgagta cctgaacacc gtgacggtgg
     1441 cggcggtcgc gacgacgctg tggctcggcg gatggcgcgg acctgctccg gacgtcctcc
     1501 cgtggttgtg gccgctgctg tggttcctgc tcaaggtgct ggtgatcgtc tacctctaca
     1561 tctggatccg cgcgacggtc ccacgcatcc gatacgaccg gctgatggcg ttcggctgga
     1621 agcgcctgat cccgttcggc ctgctgtggg tgatggtcac gggcgcgatc gtggtgctcc
     1681 ccgacgagtt cggcagggac accgtcctca cggccttggc gatcgggttc ggcgccctct
     1741 tcgtgatctc gctcgtctgg ccgctgatcg ccccgagagc ctcggaggag gtgccggtgc
     1801 cgtgagtgac gccggacgcg atcgtccgag gtccgacgtc gtatcggcgg ccggggcgat
     1861 cgcgaggggc atggggacgg tcttccggca gatcttccgc cacgacgtca ccgaggagta
     1921 tccgaaggag atcgaaccgc caccgccgcg cagccacatc ggccggcatc tcctgaaccg
     1981 gcacgagaac gggctcgaaa agtgcatcgg ctgcgagctg tgcgcgttcg cctgtccggc
     2041 cgacgcgatc tgggtcgagg gcgaggacaa cgatccc
//
LOCUS       DASUYD010000093         2549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_710603, whole genome shotgun sequence.
ACCESSION   DASUYD010000093 DASUYD010000000
VERSION     DASUYD010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710603"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2145
                     /locus_tag="VF195_05285"
     CDS             <1..2145
                     /locus_tag="VF195_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580267.1"
                     /translation="RLAAVRELAGAAAMNLAVDPELSMLLAVEAVEAARSEGESVPPD
                     VQEVLHRAIGASRIVGSVPEVGGQPDWSPTGDVFATERSDDTGVVEIRDVETGRSIRS
                     FPGHDGDITDVAFSMDGSMLATTGVDGFLKVWDPSNGAVLTEVSGVGVAEGTSFSDDG
                     KLAAAAWPDEGAVRVVNLSTDRVVRTFGELRGANGTSFGPGGVRIAVSAKFPFLNPEG
                     GTTGEVLLLDLRNNLRRILHDPRTGWQVEDVAWSPDGDLVAAGADNILRVWDAESGEQ
                     LYSIPGRTGLITSLDWWEDPSRRVLVTGSDDGTAKVWRIEEAGPKETVSLSSPEVSNV
                     TGVAFSPDGTRVIIGNDATSPGIKIWDVSAAGGAEWANLPADVFGPAFFLSERRVLAS
                     GGVGGSLSSSVTLWDVGAPSEPRRVWTIDRPAWYRDCCTPWIRASSDGATLAVFYWER
                     GTSVRDVATGDELFFVPAERPVSIPPVDLSPDGRYLAVGTDRYRAVRFLDRSGDEVGT
                     TLREDDGFSIDRFRFAPDSRLIAIESHRGAHRRVRIWDRRRGVVVTEIAVGFEDPREQ
                     EYTIYGSTLAFDPSGRLIASTVNRHVRIWDVKSGALIATLPGQPAQIGAVVFGPDGAS
                     VATGAFDGSVRVFDVDSETQSLVLHAPDAVRSVAFSPDGSLLASVDGITVRVWALDID
                     DLLEIARRNVTRSLTDEECRQYLHVEVCPPEA"
BASE COUNT          397 a          830 c          900 g          422 t
ORIGIN      
        1 cggctggccg ccgttcgtga gctcgcgggg gcggccgcca tgaacctggc cgtggacccc
       61 gagctgagca tgctgctcgc ggtcgaggcc gtcgaggcgg ctcggtcgga aggcgaatcg
      121 gtcccaccgg acgtgcagga ggtgctccac agggccatcg gtgcctcgcg gatcgtcgga
      181 tcggtgcccg aggtgggagg gcagccggac tggagcccga ccggcgacgt cttcgccacc
      241 gagagatcgg acgatacggg ggtcgtggag atccgggacg tcgagacggg acggagcatc
      301 cgctcgttcc cgggccacga cggcgacatc accgacgtgg cgttcagcat ggacggctcg
      361 atgctcgcga ccactggggt cgatggtttc ctcaaggtct gggatccatc caacggagct
      421 gtcctcaccg aggtgtccgg cgtcggcgtt gcagagggca catcgttcag cgacgacggc
      481 aagctcgccg ccgccgcctg gccggatgag ggcgcagtgc gcgtcgtgaa cctctccacg
      541 gatcgcgtcg ttcggacttt cggggaactg cgaggcgcga acgggacgtc cttcggtccc
      601 ggcggtgttc ggatcgccgt ctcggcgaag ttcccgttcc tcaaccccga gggcggcacg
      661 accggcgagg tcctcctgct cgacctgcga aacaacctgc gtcggatcct tcacgacccg
      721 cgtaccggat ggcaggtgga agatgtcgcc tggagtcccg acggcgacct cgtggccgct
      781 ggggccgaca acatcctcag ggtctgggac gcggagtcgg gtgagcagct gtacagcatc
      841 cccggccgca ccgggctgat cacgagcctc gattggtggg aggatccgtc acgccgcgtg
      901 ctggtcaccg gttctgacga cgggaccgcg aaggtctgga ggatcgagga ggccggcccg
      961 aaggagacgg tctcgctctc ctcccccgag gtgtcgaacg tcaccggggt ggcgttctcg
     1021 cctgatggaa cgcgggtgat catcgggaac gatgccacca gccccgggat caagatctgg
     1081 gatgtgagcg ctgccggcgg cgccgagtgg gcgaacctcc ccgccgacgt cttcggtccg
     1141 gccttcttcc tgtcggagcg gcgcgtgctg gcgagcggag gcgtcggggg atcgttgtct
     1201 agctcggtca ccctctggga cgttggtgcg ccttcggagc cacgccgggt ctggacgatc
     1261 gatcggccgg cgtggtatcg ggactgttgt accccgtgga tacgcgcgag ctccgatggt
     1321 gcgacgctcg cggtcttcta ctgggagcgc gggacgagcg tgcgtgatgt cgccaccggg
     1381 gacgagttgt tcttcgtccc ggcggaacgg ccggtctcga ttccccccgt cgatctgagc
     1441 ccggacggtc ggtacctcgc cgtcgggaca gaccgataca gagcagttcg attcctcgac
     1501 cgatccggtg acgaggtcgg caccacgctg cgggaagacg acgggttctc gatcgacaga
     1561 ttccggttcg ctccggacag ccggctcatc gccatcgagt cccaccgggg tgcccatcgg
     1621 cgcgtccgga tctgggatcg gcggcgaggc gtcgtcgtca ccgagatcgc cgtcgggttc
     1681 gaggaccccc gcgagcagga gtacaccatc tacggttcga ccctggcgtt cgatccgagc
     1741 ggacgcctga tcgcctcgac ggtgaaccga cacgtgagga tctgggatgt caagagcgga
     1801 gcactcatcg cgaccctgcc cggccaaccc gcccagatcg gagcggtcgt gttcggtccc
     1861 gacggcgcct ccgtcgccac cggggcattc gacgggagcg tgcgggtctt cgatgtggac
     1921 tcggagacgc agagcctggt gctgcatgct cccgacgcgg tccgcagcgt cgcgttcagc
     1981 cccgacggtt cgctgctcgc ctcggtcgat gggatcacgg tacgggtgtg ggcgctcgac
     2041 atcgacgacc tgctcgagat cgctcggcga aacgtcaccc ggtcgctcac cgacgaggaa
     2101 tgccgccaat acctgcacgt cgaggtctgc ccgcccgagg cgtagccgat cagggccgcc
     2161 gggaacccac cctggcttcg atgttgagcc tgaggttccg atcgctcagg cactcgcgag
     2221 ttcgcctttc ggcacacgca gggtcgctct aagccgtcct ccagtgcctc gatcgagctt
     2281 gagttgatcc gtggacaaga gcggcgacga acgagcgacc tggcgctcgc cgaggcaagg
     2341 ctcgataccc ggaggcatgc gggaggctgt ggtggctccc agcggctcca accggcagcc
     2401 tgtccgggaa cgcgcccccg cacggatcaa agaacgagcg gggagatcag ggccttcctg
     2461 atcgcggatg ttcgcggata taccgtcttc acccaagagc cgcattcgat gacagggtca
     2521 cggcggccgc atcaccatac gtagcccgg
//
LOCUS       DASUYD010000094         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1152883, whole genome shotgun sequence.
ACCESSION   DASUYD010000094 DASUYD010000000
VERSION     DASUYD010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1152883"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            345..593
                     /locus_tag="VF195_05290"
     CDS             345..593
                     /locus_tag="VF195_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580268.1"
                     /translation="MRPDELERCLQERLDALGPAPRAELLHVLMLPDFERADRIGEFW
                     GYPQSRAFAEPLIDCEEDLTLRAVLVGMLGEADRSGPG"
     gene            596..850
                     /locus_tag="VF195_05295"
     CDS             596..850
                     /locus_tag="VF195_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580269.1"
                     /translation="MTASRFMWIVVAGRGHSSEQRGFTEDPDDAEANVNEHAGRLRRE
                     GRTDWFGVVLDLDRPTVISPPGSTPPMLGWERVYFVDGRD"
     gene            complement(1285..1989)
                     /locus_tag="VF195_05300"
     CDS             complement(1285..1989)
                     /locus_tag="VF195_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580270.1"
                     /translation="MDRWALFVAVLALGFTAYSFFRQHGLQQRLAAIEEDRRREEIAS
                     RLQANVGARFETYASSYTAASGGARSYQLVLSNAGPARAESVTVELVGPIEGNAPDLM
                     MEGHSFPIALDPGQEYTIAAAVVVGTPPTVDVLLRWQDGTGSRREDAQPDAVRSLGGV
                     SAGTRLGVRDGRPGSVATRTEDPAKVPLRHPVRHHEIRARALRVFASAHGGTLGRTTQ
                     RGILEPVRAKGGGGGR"
BASE COUNT          400 a          755 c          721 g          391 t
ORIGIN      
        1 agcacgcctc gtgacggccg gaaggattga gagctgggtc gacaatgatc acatcctcgt
       61 gagctgcccc gttccgagat ccctagaagc ccggtctccc gagggaggcc gggcttcggc
      121 cttgacgcga cccaacgcga agagtcacct accggggctt atccgtgggc gctacaggat
      181 ttgaacccgc gtcaaggaac gccggcatat cgactgacct gcggaaacag cgaatgccca
      241 cgccagcgag gctgcaccat gtcgcaccac tcccctatgt tgcgggtgtg gtgctaccca
      301 tctgctaccc aggaacgctt gtggcacgag gaagcggtca cgacgtgcgt cccgatgagc
      361 tcgagcgctg cctgcaggag cgcctggacg cgctcggccc tgctccccgt gccgagcttc
      421 tccacgtcct gatgcttccc gacttcgagc gagccgaccg aatcggagag ttctggggct
      481 acccgcagag ccgagccttc gcggagccgc tgatcgactg cgaggaggac ctgacgctcc
      541 gagcggtgct ggtcgggatg ctaggggagg ccgaccgctc gggtccaggc tgacgatgac
      601 ggcgtcaagg ttcatgtgga tcgtcgtggc gggcagaggt cactccagcg agcagcgtgg
      661 cttcaccgag gacccggacg acgcggaggc gaacgtgaac gagcacgccg ggcggcttcg
      721 tcgagagggc cggaccgact ggttcggcgt cgtcttggac ctggatcggc cgaccgtcat
      781 ctcgccgccc ggcagtaccc cgccgatgct cggctgggag cgcgtgtact tcgtcgacgg
      841 gcgcgactga gcgcgacctt cgccgagctg ctcagcgatc gcgaggagga tcggacgctc
      901 cgggccgtgc tggtcagcat gctgcggatt ggcgaccgag gtcgttaggc ccccaaaacg
      961 tacgtaacct gtagccagca accgggcacg agcgtagcgc ggagcgccat gaaatctagg
     1021 tatcagtcat tcgaccgaca gcaccggccg tatgtaacga gctgtggcca cgagtcatag
     1081 tcgattcgtc gagctggaga ggatcatgac gatcctactc acacaggatg aacaactagt
     1141 tctacggcat ctcatctcgg gcaagagcct tgaggagatc gctgccactc tcgactggcc
     1201 gctcccggtg gtgcggaact gcatgcacac gctcatcaca tgggtgctag acgaacttga
     1261 tgcaagtgga cgaagtccaa gcaactatct cccgccccct ccccctttcg cacggacagg
     1321 ctcgagtatt ccccgttgag tcgttcgtcc gagcgttccg ccgtgagctg acgcaaacac
     1381 ccggagagct cgagctcgga tctcgtggtg tcgcactgga tggcggagcg gaaccttggc
     1441 cggatcctcc gtgcgggtcg ccacggaccc cggccgcccg tcgcggacgc cgagccgggt
     1501 cccggctgaa acaccgccta gcgaccgaac agcgtcaggt tgagcgtctt ctcggcgaga
     1561 gccggtgccg tcctgccacc ggagcagcac gtcgaccgtg ggtggcgtcc ccacgacaac
     1621 cgcagcggcg atcgtgtact cctggccggg atccaacgcg atcgggaacg agtggccctc
     1681 catcatcaga tcgggagcgt tgccctcgat cggaccgacc aactcgacgg tcacgctctc
     1741 cgctcgggct ggtcccgcgt tgctgaggac gagctgatag ctccgagcac caccacttgc
     1801 cgcggtgtag ctcgaggcgt aggtctcgaa cctcgccccg acgttcgcct gcaatcttga
     1861 ggcgatctcc tctcgccttc ggtcctcctc gatcgcggcg aggcgttgct gtagcccgtg
     1921 ctgtcggaag aacgagtagg ccgtgaaccc aagagcgagc accgccacga agagcgccca
     1981 tcggtccacc tctcgcctcc tccgcgcgca cgtcctgctt cgatcgtctc cgacaaccgc
     2041 gtctgcccgg gaatgagcgg ctaatgtgcc caacttgtgc ccaacaccgg ggctgtcgat
     2101 cgatgtcggg gcggcacaga tccctctgac cagaccttct atgttgtcaa gccgccagct
     2161 ccgccctcct cgcgtcggcg acgaggtgac ggtagaccac gttcgaaagt tgccgcttca
     2221 ggcagcgcat ggcctccttg tgggacttgc cggcctcccg ctgccga
//
LOCUS       DASUYD010000095         6792 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_271730, whole genome shotgun sequence.
ACCESSION   DASUYD010000095 DASUYD010000000
VERSION     DASUYD010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271730"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF195_05305"
     CDS             complement(<1..288)
                     /locus_tag="VF195_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580271.1"
                     /translation="MVVLVAVVACACAGVGAFLAVRHWPQADPALSATETIGQGLRNR
                     RGARRFLRSRMTPGTATGLALTAALAGLVVTGAVVGVLVWMIRRDAGLVNVD"
     gene            308..1183
                     /locus_tag="VF195_05310"
     CDS             308..1183
                     /locus_tag="VF195_05310"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6580272.1"
                     /translation="MILVAGFPGRVRWETHHVMEGTTLFESQPDSLSAHLAGRSRSRR
                     PLVSGAVVGIAGYVFMTALLVGLGLLLTKMLLDGAVGSWDRGLDRWFFDQRTPTFDEL
                     TVWGSRLGDTLTVVGIAAVAVLILSIGRHWAQIAFLVGALVIEVTTFVTTTFIVDRER
                     PAVPHLDAGPPTSSFPSGHAAAAIVLYVGLALITMSLVRNTIVRSLAWILAVGLPIAV
                     ALSRLYRGMHHPTDVIASMIGAAGCLAFALLATRTGVAVAESNHEAEEADAPTDRPAG
                     SPVVPADAVDVRSAR"
     gene            1180..2067
                     /locus_tag="VF195_05315"
     CDS             1180..2067
                     /locus_tag="VF195_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015305589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX6580273.1"
                     /translation="MTSVGVVAHAEKELGGGLPELRTTLAGYGINDPPWHEVRKSKHV
                     PERAQELVDQGVDLLFVWGGDGTVQRAIDALVGRPVTIAILPAGTANLLATNLGLPQD
                     LDACVRIGLHGARRSLDVGKVNGEHFAVMAGAGLDAMMIRSADAGLKDKVGRIAYVWT
                     GVKNVQLEPVRMSIEVDGRSWFEDEASCVLVGNVRDVLGGLTVFPEAAFDDGRLDVGV
                     VTADGWWEWARTLGRSVLGNVGASPFVQMTTGRTIDVRLDEPMPYQLDGGDRKKTKKL
                     KIKVRPGAISVAVPERGSR"
     gene            2064..3050
                     /locus_tag="VF195_05320"
     CDS             2064..3050
                     /locus_tag="VF195_05320"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX6580274.1"
                     /translation="MSSANAVPETRDLTGSDVKETLRHTGSMRLLRDAFERLRASDGF
                     SHARSMAFATSLILVQSVIALLGLASVLGAGRLSDTIVGMLQDVFPGPAGAVLTDAVK
                     QAHEAGAQNRSLALTVGLAGAIVTGTILLGQIERAMNRLYGIERDRPTLKKYGRAFVL
                     TISAGLLAVGAFVVMFLGQGVGSSLDSPLAKDVWNVVRWPLAFALLTGATACIFRWSP
                     RRHQPEWSWLAIGALLSVALVVIVTIVLNGIFQLSSTFGQTYGPLAGFVALLLWTLLS
                     SIALLYGVAIAAQLEAVRAGVPAPRRETAADGTTDRPLAEGVSQRAAVPTAS"
     gene            3047..4246
                     /locus_tag="VF195_05325"
     CDS             3047..4246
                     /locus_tag="VF195_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012998552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX6580275.1"
                     /translation="MSVAVDGRRPERTSEERIDEIRRKLEGVLGVPATEGNQVDVLRN
                     GDEIFPAMLEAISGARHAIDFLTFVYWRAEIGTSFAETLSERARSGVRVRVLLDAWGA
                     RPIDHDLIDMMQDAGVRVRWFRPLHRLQPTKVNHRTHRKVMIVDEEIGFTGGVGIADE
                     WRGDARNEDEWRDTHFRVRGPAVDGLRAAFLDNWLETETELYDPAIDRFPQQPQPGEA
                     VIQCVRGASEAGWSDMSTLLLALLQLAEERVRITTAYFVPGEELIERLCVAGDRGVKV
                     EILLPGPHADKRFVQQAGQSVFERLLRHGVRIWRFGPSMLHAKIMTVDGLVANVGSAN
                     FNARSTELDEEINLVALDPELVRLLDEQFEDDLDRSEEIDADRWRDRSLRRRVLEGLT
                     KPLRHKI"
     gene            4314..5204
                     /locus_tag="VF195_05330"
     CDS             4314..5204
                     /locus_tag="VF195_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6580276.1"
                     /translation="MRAIGLLGVPTNSSGKIDGVARGPTVLREHDLVDAIRGHADVRD
                     YGDVALPSASSERDAETHIIDPMGLGEMVARVRDTVALILDDGNLPLVIGGDCPLLLG
                     CLAAADRRDRLGLLFVDGHEDAYLPEQSSTGEAADMELAFALGIADASWSSELTHLLP
                     LVEAADVRILGARDADLLRAEGVPSLGDTVELTDGIRLAADPEGIVESACASLPDPWW
                     FHLDMDVLSTDALPAVDYPQRGGLGWEELATVARSALMARPRGWDVTIYNPDLDPERI
                     HARRIVAFIASVVERAVESV"
     gene            complement(5201..5977)
                     /locus_tag="VF195_05335"
     CDS             complement(5201..5977)
                     /locus_tag="VF195_05335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6580277.1"
                     /translation="MVEPTTEERERVLRILAKGGFIAPGAEADALLEASGEGVGPIED
                     LVARRLRGEPLAWITGWVRFCGLRVRVDRGVFVPRPHTEALARRAVELLPAAGIAVDL
                     CTGSGAVGVVLGSARPRASVVATDLDPVAVVCARRNGVHALVGDLDEPLPPSLRERVD
                     VLTAIVPYVPTEELHLLPSDVVANEPRLALDGGHRGTTLLVQAAEAAARWLRPGGTVL
                     LELGGDQAGEVGPRMAEVGLSEIRVHQDGEGRDRAIEARR"
     gene            6311..6466
                     /locus_tag="VF195_05340"
     CDS             6311..6466
                     /locus_tag="VF195_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580278.1"
                     /translation="MRVRPSGLVVEHPLRDEPWGQRRFGLRDPAGVWVDVVEQIDPEP
                     GYWDPYT"
     gene            complement(6495..>6792)
                     /locus_tag="VF195_05345"
     CDS             complement(6495..>6792)
                     /locus_tag="VF195_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020578761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX6580279.1"
                     /translation="VDRDQLLTDVSIYWFTGTGASASRFIYEAAHADRDWGAMSPAPT
                     GMAVFAADNLLRYVLNGDGHVEHWSEFESGGHFPAMEEPELLVDDIRKFFRDLR"
BASE COUNT         1040 a         2343 c         2325 g         1084 t
ORIGIN      
        1 gtcgacgttc acgagcccgg cgtcgcgccg gatcatccag acgagcacac cgacgacggc
       61 ccccgtgacg acgagaccgg ccagtgcggc cgtgagcgcc agacccgtgg ccgtcccggg
      121 cgtcatgcgc gagcggagga atcgacgcgc gccgcgacgg ttcctcaacc cctgcccgat
      181 cgtctcggtg gcgctcagcg cgggatcggc ctgcggccaa tgacggaccg cgaggaaggc
      241 cccgactccg gcgcaggcac acgcgacgac tgcgacgagc acgaccacga cgacgaacct
      301 ccttccgatg atcctcgtcg caggtttccc cggtcgcgtt cggtgggaaa cacaccatgt
      361 gatggaaggg acgaccctct tcgagagcca acccgacagc ctgtcggcgc atctggccgg
      421 ccgttcgcgg tcgcgacgcc cgctggtctc gggcgcggtg gttgggatcg ccggctatgt
      481 cttcatgacg gccctgctgg tgggcctcgg tctcctgctg accaagatgc tcctcgacgg
      541 cgcggtcggc agctgggatc gcgggctcga tcgctggttc ttcgaccagc gaacgccgac
      601 gttcgatgag ctgaccgtct ggggatcgag gctcggcgac accctcacgg tggtcggcat
      661 cgcggccgtg gccgtcctca tcctgtcgat cggccgccat tgggcccaga tcgcgttcct
      721 ggtcggagcg ctcgtgatcg aggtgacgac gttcgtgacg accacgttca tcgtcgaccg
      781 cgagcgcccc gccgtgcctc acctcgacgc cggtccgccc acgtcgagct tcccgtccgg
      841 acacgcggcg gccgcgatcg tcctgtacgt gggcctggcc ctcatcacga tgtcgctggt
      901 tcggaacacg atcgtccgct cgctcgcttg gatcctcgcc gtcggtttgc cgatcgcggt
      961 cgcgctctcg cgtttgtacc gcgggatgca ccaccccacc gatgtgatcg ccagcatgat
     1021 cggcgccgcc ggatgcctgg cgttcgcgct cctcgccaca cgcaccgggg tcgcggtggc
     1081 cgagtcgaac catgaggccg aagaggccga cgcacccacc gaccggccgg ccgggtcccc
     1141 cgtggtcccc gccgatgcgg tggacgtccg gagcgcccga tgacctccgt gggggtcgtc
     1201 gcacacgccg agaaggagct cggtggaggc ctccccgagc ttcggaccac gttggccggg
     1261 tacggcatca acgacccccc gtggcatgag gtgcggaaga gcaagcacgt gcccgaacgc
     1321 gcgcaggagc tcgtcgatca aggcgtcgac ctcctgttcg tctggggcgg cgacgggacc
     1381 gtgcagcgcg cgatcgacgc gctcgtcggg cgtcccgtca cgatcgcgat cctgcccgcc
     1441 ggcaccgcca acctgttggc caccaacctc ggccttccgc aggacctcga tgcttgcgtg
     1501 cggatcggtc tgcacggcgc ccggcgcagc ctcgatgtcg gcaaggtgaa cggcgagcac
     1561 ttcgccgtga tggccggggc ggggctggac gcgatgatga tccggagcgc cgacgccggt
     1621 ctcaaggaca aggtgggtcg gatcgcatac gtctggaccg gcgtgaagaa cgtgcagctc
     1681 gaacccgtga ggatgtcgat cgaggtcgac gggcgatcct ggttcgagga cgaggcgagc
     1741 tgcgtccttg tggggaacgt acgggacgtc ctcggtggtc tgaccgtgtt ccccgaggcg
     1801 gcgttcgacg acggccggct cgatgtcggt gtggtcaccg cggatgggtg gtgggagtgg
     1861 gcccgcacgc tgggtcggtc ggtcctcggg aacgtcggcg cttccccgtt cgtgcagatg
     1921 accaccggcc gaaccatcga tgtgcgcctg gacgagccca tgccctatca gctcgacggt
     1981 ggcgatcgga agaagaccaa gaagctcaag atcaaggtca ggccgggcgc gatctcggtc
     2041 gccgtgccgg agaggggaag ccgatgagct ctgcgaacgc cgtacccgag acccgcgacc
     2101 tgaccgggtc cgatgtgaaa gagaccctca ggcacaccgg ctcgatgcgt ctgctgcggg
     2161 acgcgttcga gcgactgcgt gcgtcggacg gattcagcca tgctcgttcg atggccttcg
     2221 ccacgtcgct gatcctggtg cagtcagtga tcgcgctcct cggactggcg agcgtgctcg
     2281 gcgccgggcg gctcagcgac acgatcgtcg ggatgctcca ggatgtgttt cccggacccg
     2341 ccggtgccgt gttgacggac gccgtcaaac aagcccacga ggccggagcc cagaaccgct
     2401 ccctggccct gacggtcgga ctggcgggag ccatcgtcac cgggaccatc ctgctcggac
     2461 agatcgagcg cgcgatgaac cggctctacg ggatcgagcg cgaccggccc accttgaaga
     2521 aatacggacg cgccttcgtg cttacgatca gcgccggtct cctcgcggtg ggggccttcg
     2581 tggtcatgtt cctcgggcaa ggggtggggt cctccttgga ctcgcctctc gcgaaggacg
     2641 tctggaacgt cgtccgctgg ccgctcgcgt tcgccctgct gaccggggcg accgcgtgca
     2701 tcttccgatg gagcccacgt cgccaccaac ccgagtggtc gtggctcgcg atcggcgccc
     2761 tgctgtccgt cgcgctggtc gtcatcgtga cgatcgtgct caacgggatc ttccaactca
     2821 gctcgacctt cggtcagacg tatgggccgc tggccggatt cgtcgcattg ctgctctgga
     2881 cgttgctgtc gtccatcgca ctcctgtacg gcgtcgcgat cgccgcccag ctcgaggccg
     2941 ttcgcgcagg cgttcccgcg ccacggcggg agaccgcagc cgacggaacc accgatcggc
     3001 cactggccga aggcgtgagc cagcgggccg cggtccccac cgcatcgtga gcgtggccgt
     3061 cgacgggcgt cggcccgaac ggacgtccga ggagcgcatc gacgagatcc gtcggaagct
     3121 cgaaggcgtc ctcggtgtcc cggcgaccga ggggaaccag gtcgacgtgc ttcggaacgg
     3181 ggacgagatc ttccccgcga tgctcgaggc catctcggga gcgaggcacg cgatcgactt
     3241 cctgacgttc gtgtactggc gcgccgagat cgggacgagc ttcgcggaga cgctgtccga
     3301 gcgagcgcgg tcgggcgttc gagttcgcgt cctgttggac gcgtgggggg cccgcccgat
     3361 cgatcacgac ctcatcgaca tgatgcagga cgcgggcgta cgggtccgtt ggttccggcc
     3421 gctccaccgg ctgcagccga cgaaggtgaa ccatcgaacc caccgcaagg tcatgattgt
     3481 ggatgaggag atcgggttca cgggcggcgt cggcatcgcc gacgagtggc gcggcgacgc
     3541 ccgcaacgag gacgaatggc gcgacacgca tttccgcgtg cgagggccgg cggtggacgg
     3601 tttgcgtgcg gcgttcctcg acaactggct cgagaccgag accgagctgt acgacccggc
     3661 gatcgaccgg ttcccccagc agccccagcc cggcgaggcg gtgatccagt gcgtccgggg
     3721 cgcatcggag gcggggtgga gcgacatgtc gaccctgctc ctggcgctgc tgcagctcgc
     3781 cgaggagcgc gtccgtatca cgacggcgta cttcgttccg ggcgaggagc tcatcgagcg
     3841 cctgtgtgtc gccggcgacc ggggcgtgaa ggtcgagatc ttgctacccg gacctcacgc
     3901 ggataagcgg ttcgtgcaac aagcgggtca gtccgtcttc gagcggttgc ttcggcatgg
     3961 tgtccggatc tggcgattcg gcccgtccat gctgcacgcc aagatcatga ccgtcgacgg
     4021 actcgtggcg aacgtgggtt ccgcgaactt caacgcgcgc tcgaccgagc tcgacgagga
     4081 gatcaacctg gtggcgctcg atcccgaact ggtgcggctc ctggacgaac agttcgagga
     4141 tgacctcgac cggagcgagg agatcgatgc ggatcgttgg agggatcgct cgctccgcag
     4201 gcgagtgctc gagggcctca ccaaacccct gcggcacaag atctgagtcc ttcgtcgtac
     4261 gcgtatctct ggcccgtccg gtcgcctgct gaggcccggg tagcttggga cggatgcgag
     4321 cgatcggact cctcggcgtg ccgacgaact cgtcgggaaa gatcgacggc gtcgcgcgag
     4381 ggccgacggt gttgcgggag cacgacctcg tcgacgccat ccgggggcat gcggacgtgc
     4441 gcgactacgg ggacgtcgcg ctgcccagcg cgtcgtcgga acgcgacgcc gaaacccaca
     4501 tcatcgatcc gatgggactc ggggagatgg tggcccgcgt ccgggacacg gtcgcgctga
     4561 tcctcgacga cgggaacctc ccgctggtca tcggcgggga ttgtcccctc ctgctggggt
     4621 gcctcgcggc cgccgaccga cgcgatcggc tcggtctcct gttcgtcgac ggccacgagg
     4681 acgcctacct tcccgagcag tcgtccaccg gcgaggccgc ggacatggag ctcgccttcg
     4741 cgctcggcat cgccgacgcg tcgtggtcct ccgagctcac ccacctgctt cccctcgtcg
     4801 aggccgcgga cgtccggatc ctcggggcac gcgacgcgga cctcctgcgc gccgaagggg
     4861 tcccgtcact cggcgacacc gtcgagctca cggacgggat ccgtctcgcc gccgatcccg
     4921 agggcatcgt tgaatcggca tgcgcctcgc tcccggatcc gtggtggttc catctcgaca
     4981 tggacgtcct ctcgaccgat gcgctccccg ccgtcgacta cccccagcgt ggcggcctcg
     5041 gttgggagga gctcgcgacc gtcgccaggt cggcgctgat ggctcgacca cgtgggtggg
     5101 acgtcacgat ctacaacccg gacctcgatc ccgagcggat ccacgcgcgg cggatcgtcg
     5161 ccttcatcgc gtccgtcgtc gaacgcgcgg tcgaatccgt ctaacgccgc gcctcgatcg
     5221 cgcggtcgcg cccctcgccg tcttgatgca cacggatctc cgagaggccc acctccgcca
     5281 tcctcggccc cacctccccc gcctggtcac cgccgagctc caagagcacg gtgccgccgg
     5341 ggcggagcca gcgcgcggcc gcttcggcgg cctgcacgag caacgtcgtc ccgcggtgcc
     5401 cgccgtcgag cgcgagacgg ggctcgtttg cgaccacgtc gctcggcagg aggtggagct
     5461 cttcggtcgg cacgtacggg acgatggccg tcaggacgtc gacgcgctct cgcaacgagg
     5521 ggggcaacgg ctcgtcgaga tcgcctacga gcgcgtggac cccgttccgc cgcgcacata
     5581 caacggccac cgggtcaagg tcggtagcca ccaccgaggc cctcggacgc gccgaaccca
     5641 ggaccacccc caccgcaccg ctgccggtgc acaggtcgac ggcgatcccg gccgccggca
     5701 gcagttcgac cgcccgccgt gccagcgcct cggtgtgcgg gcgcggcacg aacaccccgc
     5761 ggtcgacccg aacacggagg ccgcagaagc ggacccagcc ggtgatccag gcgagtggct
     5821 ccccgcgcag tcgccgtgcg accaggtcct cgatgggccc gacgccctcg ccggatgcct
     5881 cgagcagcgc gtcggcctcc gcaccggggg cgatgaagcc acccttcgcc aagatgcgga
     5941 ggacccgctc gcgttcctcg gtcgtcggct cgaccacgtc ccaagatctc ccgtcgaggt
     6001 cagctcggta tggtcgcctc ggaactgacc gagttcaatc ggaaggagtc catcatggaa
     6061 ctcgaggcct catagccggt cgtcgtaacc gacaagctga tcgagtgccg cgacttctac
     6121 acccgctggt tcggattccg ggtcgtcttc gaggccacgt ggttcgtcta tctgacctcg
     6181 tcggaggagg gccaacaggg gatcgccttc atgaccccgg accatccgtc gcggccaccg
     6241 ggtcccgaag ccttcgacag cgaaggcatc ctcctcaccc ttcaggttcc cgacggcagc
     6301 atccgagttc gtgcgggtcc gtccgtccgg actcgtcgtc gagcacccgc tccgagacga
     6361 accctggggt cagaggcgat tcggactcag agacccggcc ggcgtctggg tggacgtggt
     6421 ggagcagatc gatcccgagc cgggctattg ggatccgtac acctgaccga acaactcgag
     6481 gcgaacatct gccgtcaacg aagatcgcgg aagaacttcc tgatgtcgtc gacgagcagc
     6541 tcgggttcct ccatggccgg gaaatggccg ccgctctcga actccgacca atgctcgacg
     6601 tgcccgtcgc cgttcaggac gtaccggagg aggttgtcgg cggcgaacac ggccatcccg
     6661 gtcggcgccg gggacatcgc tccccagtcg cgatcggcgt gggcagcctc gtagatgaag
     6721 cgtgaggccg acgctcccgt gcccgtgaac cagtagatgc tgacgtcggt gagcagctgg
     6781 tcccgatcga cg
//
LOCUS       DASUYD010000096         6419 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1153683, whole genome shotgun sequence.
ACCESSION   DASUYD010000096 DASUYD010000000
VERSION     DASUYD010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153683"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VF195_05350"
     CDS             complement(<1..1262)
                     /locus_tag="VF195_05350"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6580280.1"
                     /translation="MSDTMTYPREILDVLGGRPPTEEQWRAISWPLEPYVLVAGAGSG
                     KTSVMAARVVYLALVALGRMPADHGGVLPGNVLCLTFTNKATENLQQRVRRALASLEL
                     PDGEEPEITNYHGFAAQLLDRHGVLIGIEPNQRVLTPAQRVELCGRVLDRMSFEVVEA
                     QRLPGVVDRILTLDDQAANHFRSPDEIVEFNERRLEELKEHRSDRAYGSARERIELAK
                     ACAVFRELKRELGVIDFGDQIELALNIVEQHPDVATQYRERFAAVLLDEYQDTNVAQA
                     RLMAGVFGGGHPVTAVGDPDQNIYAWRGASLFNLLEFPNQFRKADGEQATKLPLYTNF
                     RSGARILEAADVLIGQLPAAQRPDPDKRLVPWEENGPGEVHVARISDEVKEAAWVADR
                     IVALHEGGAAWSDCAVLCRTSRLFLSLQ"
     gene            complement(1259..4165)
                     /locus_tag="VF195_05355"
     CDS             complement(1259..4165)
                     /locus_tag="VF195_05355"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX6580281.1"
                     /translation="MNPPSSPDAAQDAVLSHATGALLVTGAAGTGKSSVLRERFARLV
                     EGGADPERVALIVGSRRARQGARDALITRLPASLSSLHVLTFHGLGHLILRERFSALG
                     YAEPPEVLSAADQFALVQDLLQGQDPSEWPAYGHLLTLRGFADQIRQFLLRAQEARIT
                     PEEIQAKAASRGLSGWLELARFARAYLDVLDADRVVDFAALIGRAEAAAAASEPLLDH
                     LLVDDYHDATFAQEALVQSLRPSDLVVAADPEAHVFSFQGTTAVPSLRFTQVFAGASH
                     VELTTPHRAPRTARVDAWTAAHTSEEYAAVARELRRLHVDDGVAWGELAVVIRRQGSH
                     VGGLLRALDDARVPRTAPESGVSLAQEPATRPFVLALRWLVASEDERNGLIESVLTSD
                     LVRLSPAAARGLLRIAHTRTGSVGRALEFTDGLTAEEAAHVEDVRDVLDRAAQAADRS
                     VLDAFAVLWRELPCSRRLVEAADASMDSRRELDSVVAFSSAVAEAGSAADPSVEAFLA
                     SVDAGERGPGFSAWERREVDAVQVLTAHGAVGREFHTVLVVGTVEGNFPSLTRPEPMF
                     DLEALDRLVSQSDRNRARLEDERRLFRMVVGKARERVVLMASRAHAGEASTGSRFVEE
                     LGLKWSAVPDPALAEPISVREAAALWRRTLGDRAIPGAERLAALQGLVTLGVDPRRWW
                     FQRDWTDTGRPLHEHLRASYSKLDVLDNCELQFVLGYELGLGSPVGYQAWVGSLVHRI
                     IEDCEKGAVERTLEGLVAEVDKRWRPQEFPARAVSEAWRSLAKKKMLPNWFERYGPHP
                     ASATEGGFEFDYEGASIVGYIDRIGPDPAGFPGSRITDYKTGSADRAPKANESLQLGI
                     YYLAVLESEEMKEFQPVSGVELSYLKGDWRTGELVTREWTVGTPDREEAYQDVMRQKL
                     SGLVGELKRLNDSGSYRPNYQANCFFCEFRSLCPLFPEGRPLFDVRSPQEATER"
     gene            complement(4176..5333)
                     /locus_tag="VF195_05360"
     CDS             complement(4176..5333)
                     /locus_tag="VF195_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX6580282.1"
                     /translation="MERRWKVASVGGIPIYITVPWLIFAAFVVYGTYDSLARTRALTD
                     QQALGWSVVNAILFYGSIIFHELSHAATARAFGLHVHGITMVFWGGYTETHSSERGPL
                     ASFLISAAGPFSTLALAGVFWIGSEISDDVLAAILGNLAFLSLLFAGLNALPGFPVDG
                     GRMLLAVVWGITKDRFLALRVAGWGGVVVGAVLGMIAIQRLRAQDGDWLFFAFIGWMM
                     IGQGLQVGKQSPILRDLSRGRVSEAMGPPPPPIPADGTLLHALETHLRADHTTEFPVV
                     EGFGRVIGSLSFSTASKVGGKDPTRGVRSAMTPRDRMRVVAPDLPLDRALEWIAGDGQ
                     ALVIDGERLVGRLTTSEVDRWYRRRISGETTPEDAAPVPPRPDLGGVGDWR"
     gene            complement(5388..6305)
                     /locus_tag="VF195_05365"
     CDS             complement(5388..6305)
                     /locus_tag="VF195_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX6580283.1"
                     /translation="MTDGAILIGTSSWTDPTLIKDGNFYPPGTKSAEGRLKFYASKFP
                     LVEVDSTYYFPPSERNSELWIERTPKDFTFNIKAYSLLTNHPTRPDSLYGDIKAELPK
                     ELLDKRRLYRDKLPDDAVDEVWERFRLALYPLHSAGKLGAVLFQFPQYFTISRKNKAY
                     IEEAASRLPDYRIAIEFRHKSWLEDRNVEETLSFLEERGLPLVCVDMPQGFDSSMPPI
                     ATATAKDLSMVRFHGRDPKAWETKSESASERFRYDYEKDELEGWVPKIEGLAGDTRET
                     HVLMNNCYRDFAVRNARDLGAMLDLDLPE"
BASE COUNT         1012 a         2236 c         2198 g          973 t
ORIGIN      
        1 tgctgcaacg agaggaacag ccgtgaggtg cggcacagga cggcgcagtc cgaccacgca
       61 gccccgccct cgtgcagcgc cacgatccga tcggcgaccc aggcggcctc cttcacctcg
      121 tccgagatgc gggcgacgtg cacctcaccg ggtccgttct cctcccacgg gaccagccgc
      181 ttgtcggggt cggggcgctg ggccgccggg agctgcccga tcaagacgtc ggccgcttcg
      241 aggatccgcg ctcccgatcg gaagttggtg tacaggggca gcttggtcgc ctgctcgccg
      301 tcggccttgc ggaactggtt ggggaactcc aggaggttga agagcgaggc gccgcgccac
      361 gcgtagatgt tctggtcggg gtcgccgacc gccgtgaccg ggtggccgcc gccgaacaca
      421 cccgccatca ggcgcgcctg cgccacgttc gtgtcctggt actcgtcgag cagcaccgcg
      481 gcgaaccgct cccggtactg cgtggcgacg tcgggatgct gttccacgat gttgagcgcg
      541 agctcgatct gatccccgaa gtcgatcacg ccgagctcgc gcttcagctc gcggaagacg
      601 gcgcacgcct tcgcgagctc gatgcgctcg cgagccgatc cataggcgcg gtcggatcgg
      661 tgctccttga gctcctccaa tcggcgctcg ttgaactcga cgatctcgtc cggagagcgg
      721 aagtggttcg ccgcctggtc gtcgagcgtg aggatccgat cgacgacacc ggggagacgc
      781 tgtgcctcga cgacctcgaa cgacatgcgg tcgagcaccc ggccacagag ctcgacccgc
      841 tgcgccggcg tgagcacccg ttggttcggc tcgatcccga tcaggacgcc gtgccgatcc
      901 agcagctggg ccgcgaagcc gtggtagttg gtgatctcgg gctcctcgcc gtcggggagc
      961 tcgaggctgg ccagggcacg ccggacgcgt tgctggaggt tctccgtggc cttgttcgtg
     1021 aacgtcagac acagcacgtt cccgggcagg acgccgccgt gatcggcggg catccgtccg
     1081 agcgccacga gcgcgaggta caccacgcgc gccgccatca ccgacgtctt ccccgagccg
     1141 gcaccggcca cgagcacgta cggctcgagc ggccacgaga tcgcgcgcca ctgctcttcc
     1201 gtgggaggcc tcccgccgag gacgtcgagg atctctcgcg ggtacgtcat ggtgtcgctc
     1261 atcgctcggt cgcctcctgg gggctcctta catcgaacaa cggccggccc tccgggaaga
     1321 gcgggcagag cgatcggaac tcacagaaga agcagttggc ctggtagttc ggtcgatacg
     1381 aaccggagtc gttcagtcgc ttcagctcac cgaccagacc ggagagcttc tggcgcatca
     1441 cgtcctggta cgcctcctcg cggtcgggcg tccccaccgt ccactctcgc gtcaccagct
     1501 caccggtgcg ccagtcgccc ttcaggtagg agagctccac gccggacacc ggttggaact
     1561 ccttcatctc ctctgattcg aggaccgcca ggtagtagat gcccagttgg aggctctcgt
     1621 tggccttcgg ggcccgatcg gcgctgccgg tcttgtagtc cgtgatccgg gaacccggga
     1681 acccggctgg gtcgggtccg atccggtcga tgtatccgac gatcgacgct ccctcgtagt
     1741 cgaactcgaa gcccccttcc gtggccgagg cggggtgggg gccgtaccgt tcgaaccagt
     1801 tggggagcat cttcttcttg gcgagcgaac gccacgcttc cgagacggct ctcgcgggga
     1861 actcctgagg gcgccaccgc ttgtcgacct ccgcgacgag gccctcgagc gtccgctcga
     1921 cggcaccctt ctcgcagtcc tcgatgatcc ggtggacgag cgagcccacc catgcctggt
     1981 acccgacagg gctcccgaga ccgagctcgt agccgaggac gaactgcagc tcgcagttgt
     2041 cgaggacgtc gagcttcgag tacgaggccc ggaggtgctc gtgcaacggc cggcccgtgt
     2101 ccgtccaatc gcgctggaac caccaccggc gaggatccac gccgagcgtg acgagcccct
     2161 ggagggcggc gagccgctcg gctccgggga tcgcgcgatc ccccagcgtc cggcgccaga
     2221 gggcagctgc ctctcggacg gagatcggct cggcgagagc cgggtccggg acggccgacc
     2281 acttgagtcc cagctcctcg acgaatcgtg agccggtgga cgcctcgccc gcgtgcgccc
     2341 ggctggccat cagcaccacg cgctcccgag ccttgcccac gaccatacgg aagagccgac
     2401 gctcgtcctc cagccgggcg cggttgcggt ccgattgcga cacgagccgg tcaagtgcct
     2461 ccaggtcgaa catcggctcg ggccgcgtca ggctggggaa gttcccctcc accgtgccga
     2521 ccacgaggac cgtgtggaac tcgcgtccca ccgcaccgtg ggccgtcagc acctggacgg
     2581 catcgacttc ccggcgctcc cacgccgaga acccgggtcc gcgttccccc gcatccaccg
     2641 aggcgaggaa ggcttcgacc gacggatcgg cggcggagcc tgcctccgcg acggcgctcg
     2701 agaaggcgac cacggagtcg agctcgcgcc gcgaatccat ggacgcatcg gccgcctcga
     2761 ccaatcggcg cgagcagggc agctcccgcc acaggaccgc gaaggcatcc agcaccgagc
     2821 ggtccgcggc ctgagcggct cggtcgagga cgtctcgcac gtcttccacg tgcgcggcct
     2881 cctcggcggt gagaccgtcc gtgaactcga gcgcccgccc cacggatccc gtcctcgtgt
     2941 gggcgatgcg caggaggccg cgcgccgccg cgggcgacag acgaacgaga tccgacgtga
     3001 ggacggattc gatcagcccg ttccgctcgt cctcggacgc gacgagccac cgcagcgcga
     3061 gcacgaacgg ccgggtggcc ggctcctgcg cgagcgagac cccgctctcg ggtgcggtcc
     3121 gtggcacgcg cgcgtcgtcg agcgcccgca gcagcccgcc gacgtgcgag ccctgccggc
     3181 ggatcacgac cgcgagctct ccccacgcca ctccgtcatc gacgtggagc cgccggagct
     3241 cccgtgcgac ggccgcgtac tcctccgagg tgtgtgcggc ggtccaggca tcgacgcgcg
     3301 ccgtccgtgg ggcgcgatgg ggcgtggtca gctccacgtg cgacgctccg gcgaacacct
     3361 gcgtgaaccg gagggacggg acggcggtcg tgccctggaa cgagaagacg tgcgcctccg
     3421 ggtccgcggc gacgacgagg tcgctcggtc ggagcgactg cacgagcgcc tcctgcgcga
     3481 acgtggcgtc gtggtagtcg tcgacgagca gatggtcgag gagcggctcg gacgcggccg
     3541 ccgctgcttc cgctcgaccg atcagggccg cgaagtcgac gacgcgatcg gcatccagca
     3601 cgtcgaggta ggcccgggcg aaccgcgcga gttcgagcca gccgctgagt ccgcgcgacg
     3661 cggccttcgc ctggatctcc tcgggcgtga tccgcgcctc ctgggcgcgg aggaggaact
     3721 gccggatctg gtccgcgaac ccccgcaagg tcagcaggtg gccgtacgcc ggccactcgg
     3781 aggggtcctg tccttggagc aggtcctgga cgagcgcgaa ctgatcggcc gcagacagca
     3841 cctccggcgg ctccgcgtag ccgagagccg agaagcgctc tcgaaggatc aggtgaccga
     3901 ggccgtggaa ggtcagcacg tggaggctgg acagcgacgc cggcagccgc gtgatcagag
     3961 cgtcgcgcgc gccctgacgc gcgcggcgcg atcccacgat cagtgcgacg cgctcgggat
     4021 ccgcgccgcc ctccacgagc cgcgcgaacc gttcgcggag caccgaggac ttgccggttc
     4081 cggcggcgcc ggtgaccagg agcgcacccg tcgcgtgcga gaggacggcg tcctgcgcag
     4141 catcgggaga agaggggggg ttcacgaggc ggatgctacc gccagtcgcc gacaccaccg
     4201 aggtcgggtc gtggtggtac cggcgccgcg tcctcgggcg tggtctcccc ggagatccgg
     4261 cgccggtacc agcggtcgac ctcgctcgtc gtgagccgcc ccacgagccg ttcaccgtcg
     4321 atgaccagcg cctggccgtc gccggcgatc cactcgaggg ctcgatcgag gggcaggtcc
     4381 ggcgccacga cgcgcatccg atcccggggc gtcatcgcgg atcggacccc ccgcgtgggg
     4441 tccttccctc cgaccttcga ggcggtcgag aacgagagcg agccgatcac gcgcccgaac
     4501 ccctccacga ccgggaactc cgtggtgtgg tcggctctca ggtgcgtctc gagggcgtgg
     4561 agaagcgtcc cgtccgcagg gatcggcgga ggaggtggtc ccatcgcctc cgagacccgt
     4621 ccgcgggaca gatcgcgcag gatcggtgac tgcttcccga cctggagccc ctggccgatc
     4681 atcatccaac cgatgaacgc gaagaagagc cagtcgccgt cttgcgctcg aagccgctgg
     4741 atcgcgatca tgccgaggac ggcgcccacg accacgccgc cccaccccgc cacccgcagg
     4801 gcgaggaacc ggtccttcgt gatcccccat acgacggcca ggagcatgcg accgccgtcg
     4861 accgggaacc ccggcagcgc gttcagcccg gcgaagagga ggctgaggaa cgcgaggttg
     4921 ccgaggatcg ctgccaggac gtcgtcggag atctccgatc cgatccagaa cacgcccgcc
     4981 agggccaggg tgctgaacgg gcccgccgcc gagatgagga acgacgcgag cgggccccgc
     5041 tcactcgaat gggtctccgt atagccgccc cagaacacca tcgtgatgcc atgcacgtgc
     5101 aacccgaacg cgcgggcggt cgccgcgtgc gagagctcgt ggaagatgat gcttccgtag
     5161 aagaggatcg cgttcacgac cgaccacccc agggcctgct ggtcggtcag ggcgcgcgtc
     5221 cgcgcgaggc tgtcgtaggt cccgtacacg acgaacgccg cgaagatgag ccacgggacc
     5281 gtgatgtaga tcgggatacc gccgaccgac gcgaccttcc agcgcctctc cacggtcggc
     5341 agcctacccg cgctcgcccg gaagctcgcg tcctgagtag cgatcgacta ctcgggcaga
     5401 tcgaggtcga gcatcgcgcc gagatcccgc gcgttccgca cggcgaagtc ccggtagcag
     5461 ttgttcatca ggacgtgcgt ctcccgcgtg tcgccggcga ggccctcgat cttcggaacc
     5521 catccttcga gctcatcctt ctcgtagtca tagcggaagc gctcgctcgc gctctcgctc
     5581 ttcgtctccc acgccttcgg gtcacgcccg tggaagcgga ccatcgagag gtccttcgcc
     5641 gtggcggtgg cgatcggtgg catcgaggag tcgaacccct gcggcatgtc gacgcacacg
     5701 agcggaagac cgcgttcctc gaggaacgac agcgtctcct cgacgttgcg atcctccagc
     5761 cacgacttgt gtcggaactc gatggcgatc cggtagtccg gaagcctcga ggcggcctcc
     5821 tcgatgtagg ccttgttctt cctcgagatc gtgaagtact gcgggaactg gaacaggacg
     5881 gcgccgagct tgccggcgga gtgcagcgga tagagggcga gacggaagcg ctcccacacc
     5941 tcgtccaccg cgtcatcggg gagcttgtca cggtagagac gacgcttgtc gaggagctcc
     6001 ttcgggagct ctgccttgat gtcgccgtag agcgagtccg gccgagtggg gtggttcgtg
     6061 aggagggagt acgccttgat gttgaacgtg aagtccttgg gcgttcgttc gatccagagc
     6121 tcggagttcc gttcggacgg cgggaagtag tacgtcgagt ccacctcgac caacgggaac
     6181 ttggaggcgt agaacttgag cctcccttcc gcggacttcg tccccggcgg gtagaagttg
     6241 ccgtccttga tcagcgtggg atccgtccac gacgacgtac cgatcaggat ggcgccgtcg
     6301 gtcacgactc ggtctcgccg gcgttctcat cggacttctt ccggaggagc atgtccttcg
     6361 cctgcgtggc cttctcttgc gcgatcctca gcagccgttg ggcccagacg tactccgtg
//
LOCUS       DASUYD010000097         4165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1815301, whole genome shotgun sequence.
ACCESSION   DASUYD010000097 DASUYD010000000
VERSION     DASUYD010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4165
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815301"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..939)
                     /locus_tag="VF195_05370"
     CDS             complement(<1..939)
                     /locus_tag="VF195_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580284.1"
                     /translation="MRSGTTSPPAGQPDELSPTELAARAGISPEEVERLVTCGIVVRR
                     EGSRPFRAIDALKIRVALACEEGGLPMEGMAEAVRAGLLSFAFVESWPFERWSSRRPQ
                     THLELAEEVGLSFEALQRLVEAFGFARPEPKDVVVEAERPIALLAGRIVELEIVDEAS
                     LARLGHVYAEAFRRIALAETEVYHSGVEEPLLRSGLGESRTMELASSLSPALTGMLDD
                     AVMAAYRRQQELTWTEHQIEHVEQALEDAGVSLPATPPTAMCFLDLSGYTRLTEERGD
                     QEAAALAGRLSDIVQQRSRLHGGEAVKWVGDGVMFRF"
     gene            1343..2224
                     /locus_tag="VF195_05375"
     CDS             1343..2224
                     /locus_tag="VF195_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580285.1"
                     /translation="MRRLVLFVVLATCAAFTLPQIASAAVPKCFGEKATIVGTTKADH
                     LKGTAKADVIVGLGGSDVIKGLGGSDLICGGNGSDRLIGGDRGDALVGEAGNDTLSGG
                     GGLDGLYGGAGNDRFNGGTGFDGASYFFAPSSVQVDLTAGTATGGEGTDTLTGIEDLE
                     GSRFDDTLTGNSGENFFFPGEGNDTVNGGGGTTDRVDFFFSPNPVMVDLTAGTATGEG
                     TDTLTGIGQVFGSRHDDTITGDANANTLLGGPGNDTVSGAAGDDTLDGGDGTDTLDGG
                     LGTDTCTTGETVTNCEA"
     gene            complement(2221..2826)
                     /locus_tag="VF195_05380"
     CDS             complement(2221..2826)
                     /locus_tag="VF195_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019432074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580286.1"
                     /translation="MSEVVVNTNVTLDGVMQAPAGPDEDTRGGFVHGGWARPYFDQVM
                     AEQAGEGIAKGGAFLFGRRTYEHFASVWPHQPDDDMFAKVLNERTKYVASRTLREPLE
                     WRNSTLLKGDAADAVAELKRREPKDLVILGSGELIRSLMALDLIDEYRLLIHPLVLGS
                     GRRLFTDATFATLRLVDSTTTTTGVTIATYRPAEPNAGTTT"
     gene            complement(2879..3748)
                     /locus_tag="VF195_05385"
     CDS             complement(2879..3748)
                     /locus_tag="VF195_05385"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEX6580287.1"
                     /translation="MSTTEERTFGGQTQVASLRRVLVRAPGSVDLGRWPEFGWLAEPE
                     PALAAEQHEVFCGILADAGAEVLFASSNVADDPDAVYVCDPAIMSDRGAMVLRLGKEG
                     RRSESTAIAEDLTALGVPVAFEMAKPATAEGGDTMWLDERTLLVGRSYRTNDAGIQAL
                     REALRRVEVLAFDLPRLKGPDACLHLLSLLSPLADDLVVAYVPLMPIRLLELLAEREI
                     EIVEVPEDEFESMGPNVLALAPRVALAVDGNPKTRRRMERAGVEVIVYEGSDISHKGE
                     GGPTCLTRSLLRG"
     gene            complement(3863..>4165)
                     /locus_tag="VF195_05390"
     CDS             complement(3863..>4165)
                     /locus_tag="VF195_05390"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HEX6580288.1"
                     /translation="DPVTRAPLPPRIDGIRAVLRLDDRCWVVAVADGDTLEPGTTLEA
                     GERLVFRADRILQLELGSAGAVDLEVNGETVRTGTLGEVVTLELRWRDGELVTTVV"
BASE COUNT          652 a         1449 c         1413 g          651 t
ORIGIN      
        1 gaagcggaac atgacgccgt cgccgaccca cttcaccgct tcgcctccgt gcaggcgcga
       61 gcgctgctgg acgatgtcgg agagcctgcc cgcgagtgcc gccgcctcct ggtcaccgcg
      121 ctcctcggtc aggcgggtgt aaccggagag gtcgaggaag cacatcgccg tcggcggcgt
      181 cgccggcagc gagacgcctg cgtcctcgag tgcctgctcg acgtgttcga tctggtgctc
      241 cgtccaggtg agctcctgct gccggcggta cgcggccatg acggcgtcat cgagcatccc
      301 ggtgagcgcc gggctgaggc tcgacgcgag ctccatggtc ctgctctcgc ccaggcccga
      361 gcggagcaac ggctcctcca ctccggagtg gtagacctcg gtctcggcca gggcgatccg
      421 tcggaaggcc tccgcgtaga cgtgccccag tcgcgccaga ctcgcctcgt cgacgatctc
      481 aagctcgacg atgcgccccg cgagcaaggc gatcggtcgc tccgcctcga cgaccacatc
      541 cttcggctcg ggtcgggcga acccgaaagc ctccacgagc cgctggaggg cctcgaacga
      601 cagaccgacc tcctcggcga gctcgagatg agtctgcggc ctgcgggagc tccatcgctc
      661 gaacggccaa ctctcgacga acgcgaacga caggagtccc gcccggaccg cttcggccat
      721 gccttccatc ggcaggccac cctcctcgca cgccagcgcg acccggatct tcagggcgtc
      781 gatcgctcgg aagggccggc tcccctcacg ccggaccacg atgccgcacg tgacgaggcg
      841 ttcgacctcc tccggcgaga tgccggccct ggccgcgagc tccgtgggtg agagctcatc
      901 gggctgcccg gccggcggtg aggtggtgcc gctccgcacg tggtgatcgt acgtcgtggc
      961 gagagcggcc cgggcgtcct ggccttccgt ccgggggatc tccggacgcc tcgtgatacc
     1021 gatttcgata tcagccggtt tgggtaacga tgctggaacc cttacccgcc accggttaac
     1081 acgatccccc gccggcttcc gtcttcaact tctacgtcaa gcccgtgcga gtcccggagc
     1141 cggaacgctg cacacgtgca gccttcctgt gagccccttc atgtcccatc tcatgaggtc
     1201 ggtcgtggaa aaacggtcct accagtcggt gggacttgca catctaccac cgaacgccca
     1261 gcatggggca ggttcggttc gcatcggagg gcgcacgctc ccccacgggg aggagggcac
     1321 cccccaaggg gaggtgggga agatgcgaag gctggtcctt ttcgtggtcc tggcgacctg
     1381 cgccgcgttc acgctgccgc agatcgcctc ggccgcggtg ccgaagtgct ttggggagaa
     1441 ggccacgatc gtcggtacca caaaggccga tcacctcaag ggaacggcca aggccgacgt
     1501 gatcgtcggc ctcggtggta gcgacgttat caagggtctc ggcggcagcg acctgatctg
     1561 cggcgggaat ggatcggaca ggcttatcgg cggagaccga ggcgatgcgc tggtcggcga
     1621 agcggggaac gacaccctga gtggtggagg aggcctcgac ggcctctatg gcggcgcggg
     1681 gaacgacagg ttcaacggcg gtaccggctt cgacggggcc agctacttct tcgcgccatc
     1741 aagcgtgcag gtcgatctca cggccggcac ggcgacgggc ggtgagggta ccgacacgct
     1801 gacggggatc gaggacctcg agggttcccg cttcgacgac accttgaccg gtaactccgg
     1861 ggagaacttc ttcttcccgg gcgagggcaa cgacacggtg aacgggggcg ggggcaccac
     1921 cgaccgggtc gacttcttct tctcccccaa tccggtcatg gtcgacctca ccgcagggac
     1981 cgccacgggt gagggaacgg acaccctgac ggggatcgga caggtcttcg ggtcccggca
     2041 cgacgacacg atcaccggcg acgcgaatgc gaacacgctc ttggggggac cgggaaacga
     2101 cacggtctcc ggcgccgccg gcgacgacac gctggacggg ggcgatggga ccgacacgct
     2161 ggatggaggc ctagggaccg acacgtgtac gaccggcgag acggtcacca actgcgaggc
     2221 ctaggtggtc gtgcccgcgt tcggttcggc cgggcggtag gtcgcgatcg tcacgccggt
     2281 ggtcgtcgtc gtgctgtcga cgagccggag cgtggcgaac gtggcgtcgg tgaacagccg
     2341 gcggcccgaa cccaggacga gcgggtggat caagaggcgg tactcgtcga tcaggtcgag
     2401 cgccatcagc gaccggatga gctctccgct gccgagtatc acgaggtcct tgggctcccg
     2461 ccgtttcagc tcggcgacgg cgtcggccgc gtcgcccttc aggagcgtcg agttcctcca
     2521 ctcgagcggt tcccgcaacg tccgggacgc gacgtacttg gtccgctcgt tgagcacctt
     2581 cgcgaacatg tcgtcgtcgg gctggtgagg ccagaccgat gcgaagtgct cgtaggtccg
     2641 gcgtccgaac aggaacgccc cgcccttggc gatcccctcg cccgcctgct cggccatcac
     2701 ctggtcgaag tacggtcgcg cccatccgcc gtgcacgaag ccgccccgcg tgtcctcgtc
     2761 ggggccggca ggggcctgca tcacgccgtc gagcgtcacg tttgtgttga cgacgacctc
     2821 gctcatctcc tcgttccttc cccttgggtc acgatgctat gcgacgcggc cggagcgttc
     2881 agccgcggag cagcgacctc gtgagacagg tcgggccgcc ctcgcccttg tgcgagatgt
     2941 cggagccctc gtacacgatc acctcgacgc ccgcgcgctc catccggcgc ctcgtcttcg
     3001 ggttgccgtc gacggccaac gccacgcgcg gggcgagcgc gagcacgttc ggtcccatcg
     3061 actcgaactc gtcctcgggt acctccacga tctcgatctc gcgctcggcg agcagttcga
     3121 gtagccggat cggcatcagt gggacgtacg ccaccaccag gtcgtccgcg agtggcgaca
     3181 gaagcgacag cagatggagg caggcgtcgg gtcccttcag cctcgggagg tcgaacgcga
     3241 gcacctcgac cctccgcagc gcctctcgca acgcctggat gccggcatcg ttggtccggt
     3301 agctccggcc gacgaggagc gtgcgttcgt cgagccacat cgtgtcgccg ccctcggccg
     3361 tcgccggctt cgccatctcg aacgcgaccg gcacgccgag cgccgtgaga tcctccgcga
     3421 tcgccgtgga ctcggaacgc cggccctcct tgccgagccg cagcaccatc gcgccgcgat
     3481 cggacatgat cgccgggtcg cagacgtaca cggcgtcggg gtcatcggcg acgttgctgc
     3541 tcgcgaacag gacctcggca ccggcgtcgg cgaggatgcc gcagaacacc tcgtgctgct
     3601 cggccgcgag cgcgggctcg ggttcggcga gccagccgaa ctcaggccat cggccgaggt
     3661 cgaccgagcc cggcgctcga acgagcacgc gccgcaggga cgcgacctgc gtctgccccc
     3721 cgaaggtccg ttcttcggtc gtcgacatat ctcaccttcc tccaggcgat ccgagacggt
     3781 cgagcaagga cgcctccacg ggcggagtgt acgtcggccg catcgtcggc cggcgctcgg
     3841 agatcccgaa acggcgtgtg cgtcagacga cggtggtaac gagctcaccg tcacgccatc
     3901 ggagctccag cgtgacgacc tcgccgagag tgccggtgcg taccgtctcg ccgttcacct
     3961 cgaggtcgac ggcgccggcg ctgcccagtt cgagctgcag gatgcgatcc gctcggaaca
     4021 cgagacgttc acccgcttcc agcgtcgttc ccggttcgag cgtgtccccg tcggcgaccg
     4081 cgaccaccca gcaccggtcg tccagccgca ggaccgctcg gatgccgtcg atccggggcg
     4141 ggagcggagc tcgtgtcacc gggtc
//
LOCUS       DASUYD010000098         2233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1815780, whole genome shotgun sequence.
ACCESSION   DASUYD010000098 DASUYD010000000
VERSION     DASUYD010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815780"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..596)
                     /locus_tag="VF195_05395"
     CDS             complement(21..596)
                     /locus_tag="VF195_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEX6580289.1"
                     /translation="MDSQRAFAGAVLEEGHRVLVANIDGISIDEALEAAGGFRSILGI
                     FKHVAGWNAVYYSFAFDPEPRHWTQADWPRGLRDRIEPTSEYLHELVDWFETTYERWL
                     GALNTVDDLDTARPLHWGDTASLPEIVTVVAAHWLYHAGEINAILAIRRREAWEHMEE
                     VEENHISTVGHRVRPPWMADDEWTRFAAEHP"
     gene            complement(722..1390)
                     /locus_tag="VF195_05400"
     CDS             complement(722..1390)
                     /locus_tag="VF195_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580290.1"
                     /translation="MATYVLIHGAGDGGWYWHLVDAELRERGHDVVAPDLPADDESSG
                     LSEYADVVLDAIGDRTELVVVAQSFGGFTAPLVSDRVATELLVLVAGMIPSPGEPPDD
                     WWGNTGYEREGGEQDDDVIATFYHDVPPELASEARRRERAHPSARAMGEPWPLEAWPD
                     VSTRFLLCRDDRFFSPGFLRRIARDRLCITPDEIDGGHCVALSRPSELADRLESYLDP
                     RAPG"
     gene            1617..>2233
                     /locus_tag="VF195_05405"
     CDS             1617..>2233
                     /locus_tag="VF195_05405"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX6580291.1"
                     /translation="MGGERPTPREDGFSMPAEWEPHVACYMEWPTVTRKGLWADRFDE
                     AKRDYAAVANAIAAFEPVVMVCDPDQETEVRRTCDGTVEILPLAIDDSWMRDNGPIFV
                     RDAAGRVALVHFRFNSWGERFRPYDKDADVPRHVAAHLGMRRYEAPFVLEGGSFFVDG
                     EGTLVTTEQCLLNPNRNPTMSREQIEQGLRDHLGVETIVWLGRGHS"
BASE COUNT          372 a          728 c          775 g          358 t
ORIGIN      
        1 acccttcccg agggcggcaa ctacgggtgc tcggcggcga agcgcgtcca ctcgtcgtcc
       61 gccatccagg gtggccgcac tcggtgcccg acggtgctga tgtgattctc ctcgacttcc
      121 tccatgtgct cccaagcctc ccgtcgccgg atcgcgagga tcgcgttgat ctcgccggcg
      181 tggtacagcc agtgtgcagc gacgacggtc acgatctcgg gaagggatgc cgtgtccccc
      241 cagtggagcg ggcgggcggt atcgagatca tcgacggtgt tcagtgcccc gagccagcgc
      301 tcgtaggtgg tctcgaacca gtcgacgagc tcgtgcaggt actcgctcgt cggttcgatc
      361 cgatcgcgaa gcccccgcgg ccagtcggcc tgcgtccagt gccgcggttc cggatcgaac
      421 gcgaacgagt aatacaccgc gttccagccg gcgacgtgct tgaagatgcc gaggatcgag
      481 cggaacccgc cggcggcctc gagcgcctcg tcgatggaga tgccatcgat gttcgcgacc
      541 agaacccggt gcccctcctc gagcaccgct cccgcgaacg cgcgctgcga gtccatgccg
      601 cgtcaccttc cgaagacccg ttcgagtacc ggagcgacca gtttcgcatc gaccatgtgt
      661 ctcgcgcctc cgatgacctt gagggtgatc accatcgatc ggacgatcgc gagggtagtg
      721 cctaacccgg cgctcgtggg tcgaggtacg actcgaggcg atcggccagc tccgaggggc
      781 ggctgagggc gacgcagtgc ccgccgtcga tctcgtccgg ggtgatgcac aggcggtccc
      841 gtgcgatgcg gcggaggaaa ccgggcgaga agaagcggtc gtcgcgacag agcaggaagc
      901 gggtggagac atcgggccac gcctcgaggg gccacggctc tcccatcgct ctcgcggaag
      961 ggtgagcccg ctccctcctc cgcgcttcgg acgcgagctc cggtggaacg tcgtgataga
     1021 aggtcgcgat gacgtcgtcg tcttgctcgc caccctctcg ctcgtaaccc gtgttccccc
     1081 accaatcgtc gggtggctcg ccgggggacg ggatcatgcc cgcgacgagc acgagcagct
     1141 cggtcgcgac ccggtcggac acgagcggcg ccgtgaagcc accgaacgac tgcgcgacca
     1201 cgacgagctc ggtgcgatcg ccgatcgcgt ccaacacgac atccgcgtac tccgagagtc
     1261 ccgacgactc gtcatcggcc ggcaggtccg gcgccacgac gtcgtggcct cgttcgcgca
     1321 gttcagcatc cacgaggtgc cagtaccagc cgccgtcgcc ggcgccgtgg atcaagacgt
     1381 acgtggccat cggtcggccc cttcctgctc gccggggtga ttctgcgcga acgctggcgc
     1441 gggtggaagc gggagccgtt cacgagggag agccggaagc acgtgtgtcg gtctgagaga
     1501 agccgacgcc ccctacactt ggtcgaccac aactcggccg aggatgcgga tgcgggaaga
     1561 cctcaacgtc gagatcgcgg cgacgatcgg ccgaacaggg atcatggagg aaggcgatgg
     1621 gtggtgagcg tccgacccct cgggaggacg gattctcgat gccggccgag tgggaaccgc
     1681 atgtggcttg ctacatggaa tggccgacgg tgacgcgcaa gggactctgg gctgatcggt
     1741 tcgacgaagc caagcgcgac tacgcggcgg tggcgaatgc gatcgctgca ttcgagcccg
     1801 tcgtgatggt gtgcgatccg gatcaggaga ccgaggttcg ccggacctgc gatgggacgg
     1861 tcgagatcct cccgcttgcg atcgacgact cctggatgcg cgacaacggc ccgatcttcg
     1921 tgcgagacgc cgcgggccga gtggcactgg tccatttccg cttcaactcg tggggcgagc
     1981 gcttccggcc ctatgacaag gacgcagacg tgccccggca cgtcgcggct catctcggga
     2041 tgcgccggta cgaggcgccg ttcgtgctcg agggcgggtc gttcttcgtg gacggtgagg
     2101 ggacactcgt caccaccgag cagtgcctgc tgaatcccaa ccggaacccg acgatgtcgc
     2161 gggagcagat cgagcaggga ctccgcgatc acctgggggt ggagacgatc gtctggctcg
     2221 ggcgcggcca ctc
//
LOCUS       DASUYD010000099        25601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_494149, whole genome shotgun sequence.
ACCESSION   DASUYD010000099 DASUYD010000000
VERSION     DASUYD010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25601
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494149"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF195_05410"
     CDS             complement(<1..661)
                     /locus_tag="VF195_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX6580292.1"
                     /translation="MSGAFVIGCDVGSQGTNAALYGADGELVASAYEAYDLSFPHPGW
                     AEQDPDLWTAAVETVCRRLVAECPGGAAAIRGLSFGSQLDGMVVCDASGRRLRPALIW
                     MDRRAETQAAALAERISPADFYRAVGANLDSSHAVFKALWVRDEEPDVWREVASLQSP
                     GTYVLRHATGLTAIDPSNASSLALLDPRTKAWSDGALAATGIDAALLPELGDATAPIG
                     TV"
     gene            complement(686..1741)
                     /locus_tag="VF195_05415"
     CDS             complement(686..1741)
                     /locus_tag="VF195_05415"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6580293.1"
                     /translation="MRAAFMTAVNEVGIREDAEEPSLDPRGAVLRVEACGICGTDART
                     FFNGDPKAPVPWQLGHEPVGVLEEVGPDADLPPGVAAGDRVFLGSILTCGECRWCLEG
                     FQNLCEDHLLYGYDPFPGAYAERAGVPPIATKNLIPLPPELPSDLATVADPFACALNG
                     IEQLDVRLGDVVVILGTGPIGCWQAVMARDRGASKVYMTDVSGDRLEIALDAIGQAVD
                     DAWVSGDDNGVAEVLSRTGGVGADRVSVAAPAKPAQQAALEMAAKRAVVVYFAGLPKH
                     DPVSPLDMNELHYKELSVRGAYGATHRQYRITMDYLARKQDELAKVVTHRWPLEKIAE
                     AFETIRAGTGLKMVVMP"
     gene            complement(1741..2562)
                     /locus_tag="VF195_05420"
     CDS             complement(1741..2562)
                     /locus_tag="VF195_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580294.1"
                     /translation="MTGKAMRLKRVIDPAGVSVICALDHGMTSPTFLEPLADITARAS
                     ETVAGGANVIMMSKGMIAIAEPAFAPTTSLALLLSASASPGGDRPEIVQIAEVEEALI
                     LGADAVVLFTALGGDTEASMIEILSSVGRECAALGMPFIAEAEFPTTYASVEELKDLY
                     GFEYLRRNVRLCAELGADIVKTNWPGDAEGFAKLVEATNGIPVVLAGGSRLEDADLLT
                     RMEHAVAAGAIGCSVGRNIFMHRSPEAITRALSRVIRERWSADKAVAALQEELST"
     gene            2666..3382
                     /locus_tag="VF195_05425"
     CDS             2666..3382
                     /locus_tag="VF195_05425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEX6580295.1"
                     /translation="MTVILFVRCDASETFGVAPAAVESAGATVRVWNALDGEDQRPPL
                     DEIGGVVVFGSTFNVEHADEQPFIKEIADLIRETIERRIPFLGVCFGGQLLAWALDAR
                     VGEGPVREIGFEPIHPTDEAAHDLLFSHLADSDLAFQWHGDAFDLPEGATLLATGDRV
                     PNQAFRVGDLAWGTQFHCEVNRSELEFWLQEFTKVGDLRSEWGKSSEQVREEAERFMT
                     EHESRGREVFERFTNLAAGA"
     gene            3415..4719
                     /locus_tag="VF195_05430"
     CDS             3415..4719
                     /locus_tag="VF195_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX6580296.1"
                     /translation="MAEESYRPLIAVVAYHLGGDRVARWPEGGYGVPALYLDALRRAG
                     ARTVILAPGEEGDPEELLEPFDGLLLVGGGDIDPSRYGGTANEHVYGVEPDRDEAEIA
                     LLHAADRIAMPTLCICRGIQIMNVAFGGTLQGHLPDVPGLQVHGVPLDESQTIHEVSP
                     EPGSRLAAVTKSASLTCLSHHHQGIDRLGDGLAVTGRTEDGLVESIERIVPDPEDTRA
                     TWMLGVQWHPEETAGRDAAQQSLFDALTLLARLRGSRARPGEGQGRSRSYSIDPYDPG
                     WPSRYEAESARVREALGKQLVRIEHIGSTSVPGLAAKPIVDIQASVRSMIPRADYVEP
                     LRSLGYRWVIDPWSDQHEYFSRDDDGRRSFQIHVCLAGSEWERRHIAFRDWLREHPGD
                     AEAYERLKRELADRHRRDILSYVDEKTPFIRQIETRAATRTR"
     gene            complement(4729..5928)
                     /locus_tag="VF195_05435"
     CDS             complement(4729..5928)
                     /locus_tag="VF195_05435"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX6580297.1"
                     /translation="MSTLVPFPTRIVRPEWAEHVVSPMHDALSREERAEILADRPYAW
                     LHVSRTPDDAPEGQDVDPAALDEANVAALRRLLDADLFEGFPVPALYVYRLRSAGHEQ
                     TGIVGEVPPQAFVDGRILGHEGVQPERVDALGTHLSRLGARSTLVALMFRADDDVRKI
                     VRATTEAIPLRRFRSGDLEQTVWRIDDGSAVETIRERLDDHRLYITDGHHRAMASVAL
                     WERAGRPNGAGVPVVLFPDEELRMLGFHRRVVGPLPLGVDELLQRLRERLTVEEVATQ
                     PGERGVFGLYVRGRWYRLTPGGNEATAGVGSLDVSRLHRLVMEPIFGFGDAGDPGLEF
                     VSDAVPVDELMTRVDEDGGAAFTLVPPTFDQFVEVADRHEQMPAKATYFDPKPQSGLF
                     VTIDNGS"
     gene            complement(5925..7004)
                     /gene="serC"
                     /locus_tag="VF195_05440"
     CDS             complement(5925..7004)
                     /gene="serC"
                     /locus_tag="VF195_05440"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX6580298.1"
                     /translation="MRIHNFCAGPCTLPLDVLEEVQAELLDFEGSGMSVIEMSHRSAE
                     YEAVHEEALTLFRENAAVPDDFTILFLQGGATLQFAVVPMNLLGDGARAGYVRSGTWG
                     KGALKDAAIWGDAYAAWDGEPISFSRMPSDDELEIRPDTRYLHVTSNETIEGIRMSSF
                     PDIDLPLVGDMSSDYLSRPVPWERFDLVYGGAQKNLGPAGLAVMVVRRAALEEMRTDL
                     PSYLRLGAHADKHSLLNTPPMFAIYVTGKVLRWMRDRGGVAGMEERAARRSGAVYDAI
                     DRSDGFYRSPVEPSHRSHMNVVFRTPSDELDAAFLVGAEERGMRNLKGHRSVGGIRAS
                     IYNAMTDGGVDALVGFMAAFRDTAG"
     gene            complement(7001..8218)
                     /locus_tag="VF195_05445"
     CDS             complement(7001..8218)
                     /locus_tag="VF195_05445"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6580299.1"
                     /translation="MKVLLADAIHPSTVEVLTERGHDCVLEPKLSSDDLPGRMPGFQV
                     LVVRSTKVTAAAVEAGNALELIVRAGAGTNTIDVEAASEVGIYVTNVPGRNAIAVAEL
                     TMGLLLAIDRRIPDNVADLRAGEWNKSTYGKADGLFGKVMGIVGLGEIGFAVAERAGA
                     FGMALRAVRKDRDDEAEERIKSLEIELVGSLEELVGTSDVVTIHVPSSPETGSMFDAQ
                     LLGAMKEGAILLNTSRGNIVDEAALLDALETRELRAGLDVYPGEPGSGSASWASPLAQ
                     HPRVVGTHHIGASTAQAQKAVADGVVEIIESYVRGEILNCVNLAPTRLGTHTLHVRHL
                     DRVGVLAGVFDILRRRGLNVEQMENRVFEGRNAAVATIDVVGDVEPDLIAALEGLDDV
                     IHVSAVPTDHRPT"
     gene            8474..10315
                     /locus_tag="VF195_05450"
     CDS             8474..10315
                     /locus_tag="VF195_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580300.1"
                     /translation="MKGLRRASLTLAVATAVMVGLAGSALGQSATAEDEKITFNVGTT
                     SDMVSANPFKACCSSEYEMLLLNYNMLYGFSAEDLSPVPELVEEPGCEPSEDYMTWTC
                     QIRDDVTWHDGEPLTAADIAFTYRFILDNGLTTFSDYLPFNPTFEATDDNQLIWTSEE
                     PTLAPTVPPYIPILPEHVWGPLDGQPAKEIRAFEEIPAIGSGPFQLTEWETGQFFRME
                     AVEGHFFGDPTIDEIVYTVFGNDEAMVQALKAGDIDYAYDLPPTLVDSLESEENIAIV
                     EEAADYHTNLAFNFGGQAEAHPKVYGTPPATQETNHPALHDHAVRLAIAHAIDKEALA
                     EVVFQGAAQPADTFISPDKAFWHLDIPAEEEYAFDLEEANRILDEAGYTERTADGVRI
                     DPESGEPLVLDIVAINNSRGSARSGELMAGWMEEIGIEFDVRVVSESKAYVEWENGTF
                     DAYIWSWGGDPDPDFNMSIYTSDQCLGWSDGCYVNEELDQLYAEQRQTFDREARQELV
                     HEFQRIHYEEIPEIALVYPELLHAYRTDTFEGYVNSPTNGGAPIFAWRVDSYMNLQPV
                     TAGGPGGNVADEGGMSTGLLVAIGAIVVIAIGAFVVMGRRRSEEDEA"
     gene            10353..11321
                     /locus_tag="VF195_05455"
     CDS             10353..11321
                     /locus_tag="VF195_05455"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580301.1"
                     /translation="MGSRRYLLRHVLHALATLAFVLAFNFVLFRAIGDPVKLLTRSSL
                     HLDPQEQEALRQEFGIDDPLPAQFVNYLGDTLRGEFGYSFISGRPVIRSVAERVWPTV
                     VLVGTATILSTFFGVLIGIKGAWQRGSTFDTGSLLGSLTLYSMPEGWLGMMLLILFTG
                     TLGVLPAGGYQSSAQLSGFGHFADVGKHLILPCLTLTLGYIGEFAIIMRSSLLEVMGE
                     DFVQTARAKGVRDKDVRRRHAVPNALLPTFTLAFYSVGFILGGAVIIEEVFSWPGLGQ
                     LTYAAIEANDYPVIQAVFLLSSAAVILFNLLADILYGYLDPRVKEG"
     gene            11323..12276
                     /locus_tag="VF195_05460"
     CDS             11323..12276
                     /locus_tag="VF195_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018569381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580302.1"
                     /translation="MIEPRQPSEVAVGTEVAAPPPSRDPKKIRRARRRRAFSGFWRVY
                     RASRMGMVGLVLLGIFVLMAFTAPLIVSDEELSVSTPSGGPLEPPSAEFPLGTDDFGR
                     SVLDLVIYGSRISLLVGFAATVMTMTVGAAVGITGGYFGGRVDSIMNAFTNWFLVIPW
                     IPLAIVLASVLGRSLFNTILVIGITSWAGTARLVRADTLSVKERPYVERARALGASNW
                     HLVTRHILPNVMPVIFANTVLTVALSILAETTLALLGLGDPFSTSWGVIIFDAFEQGA
                     LSAGKWWWLIPPGVCICLVTLAFTMCGYALDEVLNPRIRER"
     gene            12281..13267
                     /locus_tag="VF195_05465"
     CDS             12281..13267
                     /locus_tag="VF195_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018521460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580303.1"
                     /translation="MSVLSTRDLRVTYRTKAGDVPAVRGVDLNIDRGQVLGLAGESGC
                     GKSTIGGAILRLHAPKTRIEGSILLDGNDVLSLKPGRLRAVRWTGASIIFQGALHAMN
                     PVRRIGEQVAEAITVHKLAGEREARVRTAELLEQVGLPGRRIDDYPHELSGGQKQRVM
                     IAMALACNPSLVIADEPTTALDVMVQAQVLKLLKALQRELGLAMLFITHDLSVLVEVA
                     DRIAIMYAGLIVEEGPAERVFRSPQHPYTKALAGAFPEIGDTQFRRKPSGLGGDPPDP
                     QQIPSGCPFHPRCSEAIDVCPSLDVDLWAAGEGRRAACIHVEGAPSPAEARS"
     gene            13300..14322
                     /locus_tag="VF195_05470"
     CDS             13300..14322
                     /locus_tag="VF195_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017559906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580304.1"
                     /translation="MGEPIIEVRDVEVSFTGRLSLLKGLGGGKGTIARAVDGVELTLH
                     EGEVLALAGESGCGKTTLARAIMGLIRPKAGEISFRGVRLGRSLREYRREVQMIFQDP
                     TASLNPRQTIYESVAEGLRIHKIHQGPDGETEEQLVAQALSRAGMRPPERFYLLYPHE
                     LSGGQRQRVVIAGALVLDPKVIVADEPVSNLDASVRGEILQLLLKLRDELRLSILIVT
                     HDLGLAWTVADRVAVMYLGRIVEIGPAEEVLLRPRHPYTKALLDVVPEAGGIDRPILS
                     GEPPDPTRVPPGCRFHPRCPVVASGRAATLGIEQRCKGEDLGLEELAPGHLAACYEAE
                     LTVASR"
     gene            complement(14331..15014)
                     /locus_tag="VF195_05475"
     CDS             complement(14331..15014)
                     /locus_tag="VF195_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580305.1"
                     /translation="MNEQSLERVNAFLAAVAQRTQKGELLEATPAELGRGLGFPDPLS
                     IARAVRALVARRRLEPAGGSYRLLDDTPVAAGERETIERKRRRTKKRSEDAQPGRRSG
                     GLAYSDMGRATVDRLVELSQEVSQLRAQLKTSREESRESREMRVEAEGRARTFAERAR
                     ELEHRAEMAESNLRTLLASARATGMHPDQRLPDSEMEAILGVLKGNGDQAAPEERPVP
                     SEADEGAGA"
     gene            15095..16237
                     /locus_tag="VF195_05480"
     CDS             15095..16237
                     /locus_tag="VF195_05480"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX6580306.1"
                     /translation="MDDLPLSGCRILDLSRVLAGPLATQILGDLGADVIKIERPEQGD
                     DTRHWGPPFVGDDAAYYLALNRNKRSVALDLLSHDGADAIRSLAASSDVLIENFRPGL
                     MSEFGLALDDLRRASPRLITCSLTAFNDEIPESASRPGYDIIVQALSGLMSVTGEPDG
                     EPVKVGVALLDVITGLEMAIGILAALHERSRTGRGRHISVSLFDAGVAAMVNQAANHL
                     LGGVVARPMGTAHPNICPYQAFHASDRPFIVAAANDRLFRRTCEVVGHPEWAVDPRFA
                     TNDDRVRHRDELIPLLAESFGGKTAAEWVAALEAASVPAAPIRAIDEVFSSPEGAAMV
                     EEVADPGRGTTLRLLRNPIRFDGAGLDTRLPPPLLGEHTDEVLRER"
     gene            16234..17055
                     /locus_tag="VF195_05485"
     CDS             16234..17055
                     /locus_tag="VF195_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6580307.1"
                     /translation="MKAVERWRERLGRWAIPPEILAAAPESPWGFPTELFRARGARGA
                     DREGTTPTTARALERLPEAGRVLDVGCGGGATSLPLAGRAGTLVGVDAQEDMLDGFLA
                     NARAAGVSAVAVLGRWPEVAETIDPVDVAVAGHVLYNVADLEPFVLRLAALAHHRVVF
                     ELTDRHPLAWMNDLWRHFHGLERPTGPSSHDVLDVLGEIGLAATLERWEAPPPVGGFA
                     ERADALALARRRLCLPSSRDDELASALGPRLAERGGLWSVGPPGSQRLATIWFDP"
     gene            complement(17052..17636)
                     /locus_tag="VF195_05490"
     CDS             complement(17052..17636)
                     /locus_tag="VF195_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580308.1"
                     /translation="MRGWARVLLWVVILLACAGVGAFIGSRSNPFPPGVPDPGAQPSD
                     GPSPAPPEHDEWPITMVSRTTHVFHVGGSCTSAWRVKGTLRISASGRITGRGVARLQP
                     GAGCDFPSAQVQAERILVLFLGRRDGDELDLRFRDVGRMPAGSQDLGAFVKTLETLRV
                     AIVEAAGSEVVKQTRIEDPEDEIYRSAITIRLGG"
     gene            complement(17671..18078)
                     /locus_tag="VF195_05495"
     CDS             complement(17671..18078)
                     /locus_tag="VF195_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580309.1"
                     /translation="MTALHRVIGFAVVAIFALGWIWGLGARIARRDPSEGYWTWLAIA
                     QVVAGVQAIVGTILLLLGHRLEAEGALGGALHYVYGYLPLLLFVFAHVVARAGDARAV
                     GIDRPLRPWVPFAWASFISFGLTLRALMTGLGL"
     gene            complement(18089..18829)
                     /locus_tag="VF195_05500"
     CDS             complement(18089..18829)
                     /locus_tag="VF195_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580310.1"
                     /translation="MSEDRPAFYALAPGGWRDWWTLLHPPYTLWHLSYVALGASTAAE
                     LNLYRLGLSLLGFFLGVGLAAHALDELRGRPLETSIPDGVLVAVAAVSLAGAAAVGTI
                     GIVQVTGWLALFIVVGAFLVVAYNLELLGGAFHSDLWFALAWGGFPALTGAFAQDGRL
                     GLAAVAAAAACVAISAAQRALSTPVRRLRRRVASVEGTITLRDGTTEPIGPEAIRRAP
                     EAALQLLSIAMPVLAVAFVIAKRSSPVP"
     gene            complement(18826..19542)
                     /locus_tag="VF195_05505"
     CDS             complement(18826..19542)
                     /locus_tag="VF195_05505"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6580311.1"
                     /translation="MAVERTRFAKRLFAGIATQYERMGALLSFGQDARWRRFLVSRSN
                     AVPGGRVLDVAAGTQLVSRALAAGRNVHVVALDQSEPMLRAGGEPNRLADLDGRIRPV
                     LGQAERLPFEDGSFDAVSFTYLLRYVEDPAATVRELARVLRAGGSIAMLEFSVPEAPL
                     ARLGWWVQTRVVMRLLGVAVSPAWARTTAFLGPNISRFVADHPIEDWVRWFQAAGIRH
                     VRSRSFLFGSAVVLWGVKSS"
     gene            complement(19553..20329)
                     /locus_tag="VF195_05510"
     CDS             complement(19553..20329)
                     /locus_tag="VF195_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015577189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01458 family HAD-type hydrolase"
                     /protein_id="HEX6580312.1"
                     /translation="MTFDGLLLDIDGVLAVSWEALPGAVDALARLRDEKVPFRLITNT
                     TTKSRANLAATLRDAGFDVADEEVVTAVVATAEHLRTAHPGAPVFVLSDGDATADLVG
                     IDLVDVDDADVIAIGGACEEFTYDTMNRIFRRLMDGAALVGMHRNLFWKTADGWELDG
                     GAYLAGLEEAADTRAAICGKPEKAYFDAGLAMLGVEADRAAMVGDDIVNDILGAQAAG
                     LTGILVRTGKYRAGDDARDGGRPDHVLDSFADVPSFLGSP"
     gene            complement(20346..21197)
                     /locus_tag="VF195_05515"
     CDS             complement(20346..21197)
                     /locus_tag="VF195_05515"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX6580313.1"
                     /translation="MDRDEGYFGPRSVSWQVHREVTVLFGGARAMLMQAAHPSVIAGA
                     NQTGMYERNPWKRLQRTLVLQYALTFGTREEAHAAADRINDVHERINGVDPVTGRRYD
                     ATDPALLLWVHACLVESALLFERLTVGRLDDRGRQRFHEEQMLAAELVRLPRDLIPPT
                     VRELEEYVADTVRSGELLVTDAARSVAGLFLDPPPDAQWRPVLRLVARLAFGTLPAEV
                     RAGYQLPHRKGERAIRRASFAAMRAGRPLLPARFRYIAPYQSWRRRRRLGAPGEPEPA
                     RSLRIRP"
     gene            21291..21899
                     /locus_tag="VF195_05520"
     CDS             21291..21899
                     /locus_tag="VF195_05520"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HEX6580314.1"
                     /translation="MLLVLIVIGLAVCVGFAAGGSLRPFERFRVHWWGVALVGLALQG
                     IPFTSGVSRPIGTAALLGSYVLLGSFAFVNRRLPAVWLVMVGLALNFVVIGVNNGMPV
                     SAGALEVSGASAEGLAGEGTLKHHLMGPGDSLTPLGDVIGIPPPVGVVISIGDVILYA
                     GVAILVVAVMLGRSGENRRPPARWFQGYRGKHLRPRPRFSRL"
     gene            complement(21796..23376)
                     /locus_tag="VF195_05525"
     CDS             complement(21796..23376)
                     /locus_tag="VF195_05525"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6580315.1"
                     /translation="MTVSGRRVHPGRVMAFELSIILPTFALGAFLISRDPSAFEDGWP
                     LVVWAIVIGLVELLPVPAWRGLTISVGFPLMMVVAFLYQPAAAAAVALVGASDPREFQ
                     REVTVLRALFNRCQVALSVLAASAVFHWMTPFAAGTSHTSAVLLLVAAASASLVDYAV
                     NSGLVTMFVSLRTGVPILQVMKELRIGRLSEFLISYLGLGLVGLSLAFLYNAADFFAL
                     PAILAPLLFARQMFFRSQALEEAHKELQEREQVLRALSNAMAEERADERLQIAGYLHD
                     DLAQVLFRLSIQVDVARKLLEKGQTDDVGAQLDKIRQSKQETSDRIRALIRDLHRSPL
                     GAKGLAEALESFTDEVGRDSSIRFHRDVADIQLPAPIALLVFHIAREGVMNAMKHAQP
                     REVWIGVREEDADIVLSLRDNGVGFDTEAPGPEGHFGMAMMRERAQVGGGRFEVQSTP
                     GQGTTIIVRFPKSLLGDEPPPAPQAPPDPHDGSGGAKPLPAGPTDGRTGDVDGGASPD
                     IPGTSEPAASDSHPTVRA"
     gene            complement(23737..25149)
                     /locus_tag="VF195_05530"
     CDS             complement(23737..25149)
                     /locus_tag="VF195_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580316.1"
                     /translation="MSVYASRRGGGRDPAAAERKPRRATAFLWVVSFLSVAATISFAL
                     RESLDPTGLVQLASWAAIVAAVDMIPVRVWGSVTVSMSFPVALAAGMIFSPAEAALIA
                     FVGSFDPRELSRHVSLAKSIFNRSQVAASVMAGAVAFHALGGTITEWPGVLVPGVVAL
                     LFDAGVNFLLVASAVAIEDRLAPRGVLLRMFGASPWHYLAGYLLLGLLAMPLSAAFAT
                     GGIWAVLLFLAPLVLAREMFRQTKQFLDATERIRLKDVAILGAADEVVRERKDERLAL
                     AGELHDEVLPSLFKVHLMGQVLRQDLAAGKLLELDDDLPELLFATDAAQRAIRDLLGD
                     LRRSPLGASGLIPTLRLLVDQLASAGAPPVTLDVEQLDSSPLAQLLAYQAIREALHNA
                     AKHAQASSIGVRVWTDEALLRLSVTDDGTGFSWTAVDGSRHFGLQIMKERFEAAGGSM
                     FVDSRLGVGTLVAASIPKNA"
     gene            25309..>25601
                     /locus_tag="VF195_05535"
     CDS             25309..>25601
                     /locus_tag="VF195_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HEX6580317.1"
                     /translation="MTTERDPDRRTRRNPFRDEVWGDEPQEVDASEFVEELETSRPDL
                     ARRWHDHPTIVPFKVVGRFIGRNSKRVAVTIAGFVVVLAGAAMLVLPGPGLLV"
BASE COUNT         4188 a         8877 c         8538 g         3998 t
ORIGIN      
        1 tcaccgtgcc gatcggcgcc gtggcgtcgc cgagctccgg gagcagagcg gcgtcgatcc
       61 cggtcgcggc cagcgccccg tcggaccagg ccttcgtgcg ggggtcgagc aacgcgaggg
      121 acgaagcgtt cgacggatcg atcgccgtga ggccggtggc gtgccgcagg acgtacgtcc
      181 cgggcgactg gagtgaggcg acctcacgcc agacgtccgg ctcctcgtcc cgcacccaca
      241 gcgccttgaa caccgcgtgc gaggaatcga ggttcgcgcc gacggcacga tagaagtcgg
      301 ccggcgagat ccgctcggcg agggccgccg cctgggtctc ggcccggcgg tccatccaga
      361 tcagcgcggg gcgcagccgg cgccccgacg cgtcgcagac gaccatcccg tcgagctgcg
      421 acccgaacga gaggccgcgg atcgcggcag ccccaccagg acactcggcc accaatctcc
      481 gacacaccgt ctccacggcc gcggtccaca gatccggatc ctgctccgcc caccccgggt
      541 gcgggaacga gaggtcgtag gcctcgtagg ccgacgccac gagctcgccg tcggcgccgt
      601 agagcgctgc gttcgtgccc tggctgccca catcgcagcc gatcacgaac gcgccgctca
      661 cggcagcctc atctctcgga tgccctcacg gcatgacgac catcttcagc ccggtgccag
      721 ctcggatcgt ctcgaacgcc tcggcgatct tctcgagggg ccaccgatgg gtgaccacct
      781 tcgcgagctc atcttgcttg cgggccaggt agtccatcgt gatccgatac tgccggtggg
      841 tggctccgta ggcacctcga acggacagct ccttgtagtg gagctcgttc atgtcgagcg
      901 ggctgaccgg atcgtgcttc ggaaggcccg cgaagtacac gaccacggcg cgcttcgcag
      961 ccatctcgag ggccgcctgc tgggcgggct tcgccggcgc cgccacgctg acgcggtcgg
     1021 cgccgacgcc gcccgtccgt gacaggacct ccgcgacgcc gttgtcgtcg cccgagaccc
     1081 acgcgtcgtc gaccgcctgc ccgatcgcgt cgagcgcgat ctccaaccgg tcgccgctca
     1141 cgtcggtcat gtagaccttc gatgcccctc gatcgcgggc catcacggcc tgccagcagc
     1201 cgatcggccc ggtccccagg atcaccacga cgtcgccgag ccggacgtcg agctgctcga
     1261 tcccgttcag cgcgcacgcg aacgggtcgg ccaccgtggc gaggtcgctc ggcaactccg
     1321 gcgggagcgg gatcaggttc ttcgtcgcga tcgggggcac accggcccgc tcggcgtagg
     1381 cgcccgggaa cgggtcgtag ccgtagagga ggtgatcctc gcagaggttc tggaagccct
     1441 ccaggcacca gcggcactcg ccgcacgtga ggatcgatcc gaggaagacg cgatcgccgg
     1501 ccgcgacccc gggcgggagg tccgcgtcgg gtccgacctc ctcgagcacg cccaccggct
     1561 cgtgcccgag ctgccaaggg acgggcgcct tcgggtcgcc gttgaagaac gtgcgcgcgt
     1621 ccgtgccgca gatgccgcac gcctccacgc ggagcacggc gccgcggggg tcgagcgacg
     1681 gctcttcggc gtcctcgcgg atcccgacct cgttgacggc ggtcatgaac gccgcgcgca
     1741 tcaggtgctc agctcctcct gcagggcggc gacggccttg tccgcgctcc agcgctcccg
     1801 gatcacgcgt gacagcgccc gcgtgatcgc ctcgggagat cgatgcatga agatgttccg
     1861 gccgaccgag cagccgatcg cgccggcggc gaccgcgtgc tccatccgcg tcaggaggtc
     1921 ggcgtcctcg aggcgggagc cgccggcgag caccacgggg atcccgttcg tggcctcgac
     1981 gagcttcgcg aagccctcgg cgtctcccgg ccagttcgtc ttgacgatgt cggccccgag
     2041 ctcggcacac aagcgcacgt tgcgccgcag atactcgaag ccgtagaggt ccttcagctc
     2101 ctcgacgctc gcgtacgtcg tggggaactc cgcctcggcg atgaacggca tcccgagcgc
     2161 cgcgcattcg cgtcccacgc tcgacaggat ctcgatcatc gacgcttcgg tgtcgccgcc
     2221 gagcgccgtg aacaggacca cggcgtcggc gccgaggatc agcgcctcct cgacctcggc
     2281 gatctgcacg atctccggcc ggtccccgcc gggactcgcg gaggccgaga gcagcagggc
     2341 caacgacgtc gtcggggcga acgccggctc cgcgatcgcg atcatcccct tgctcatcat
     2401 gatgacgttg gccccgccgg ccaccgtctc ggatgcccgg gccgtgatgt ccgcgagcgg
     2461 ctccaggaac gtgggggacg tcatcccgtg gtcgagcgcg cagatcaccg acacccccgc
     2521 cgggtcgatc acacgcttga gtcgcatcgc cttgcccgtc atcgcctcgg ccccccgttc
     2581 tcgctcggct gctcgcgcct gcgcagtgtg ccagagggcg cccgctcgcg cgacccgaga
     2641 aggtggccgg gctagactcc gctcgatgac cgtcatcctc ttcgtgcgat gcgatgcgtc
     2701 ggagacgttc ggggtcgctc ccgcggcagt ggagtctgcc ggcgcgactg tccgcgtgtg
     2761 gaacgcgctc gacggcgagg accagagacc gccgctcgac gaaatcggtg gggtcgtcgt
     2821 cttcggctcc acgttcaacg tcgagcacgc cgacgagcag ccgttcatca aggagatcgc
     2881 cgacctgatc cgcgagacca tcgagcgccg gatccccttc ctcggcgtct gcttcggtgg
     2941 gcagctcctc gcctgggcct tggacgcgag ggtcggcgag gggcccgtgc gcgagatcgg
     3001 cttcgaaccg atccatccca ccgacgaggc ggcgcacgat ctcctcttct cgcacctcgc
     3061 ggacagcgac ctcgcgttcc agtggcacgg ggatgccttc gatctccccg aaggagccac
     3121 gttgctcgcc acgggcgatc gcgtccccaa tcaggccttc cgggtgggag atctcgcgtg
     3181 gggcacgcag ttccactgcg aggtgaaccg atccgagctc gagttctggc tccaggagtt
     3241 cacgaaggtc ggcgatctcc gatcggagtg gggcaagagc tctgagcagg tccgcgagga
     3301 ggcggagcgg ttcatgaccg aacacgagag ccgcggccgc gaggtgttcg agcgcttcac
     3361 caacctggcc gcgggcgcgt aggccgccgg ggggccgtcg ctacactcga cgtcgtggcc
     3421 gaagagagct accgacctct catcgccgtg gtcgcgtacc acctgggggg cgatcgcgtt
     3481 gcccggtggc ccgagggcgg gtacggcgtc ccggccctgt acctggacgc gctccgccgc
     3541 gccggcgctc ggacggtgat cctcgctccg ggtgaggagg gggatccgga agaactcctc
     3601 gagccgttcg acgggctcct gctcgtcggc ggtggtgaca tcgacccgtc gcggtacggc
     3661 ggaaccgcga acgagcacgt ctacggcgtc gagccggacc gcgacgaagc cgagatcgcg
     3721 ctgcttcacg ccgccgatcg gatcgcaatg ccgacgctct gtatctgccg cggcatccag
     3781 atcatgaacg tcgcgttcgg aggcaccctg caaggacacc tccccgacgt cccgggcttg
     3841 caggtgcacg gtgtgccgct cgacgagagc cagacgatcc acgaagtgag ccccgaaccg
     3901 gggagccgac tcgccgccgt cacgaagtcg gcgtcgctca cctgcctctc ccatcaccat
     3961 caagggatcg accgcctggg ggacggcttg gccgtgacgg gccggaccga ggacggcctg
     4021 gtcgagtcga tcgagcggat cgttcccgac cccgaggaca cgcgcgcgac ctggatgctc
     4081 ggcgtgcaat ggcacccgga agagaccgcc ggccgggacg ccgcccagca gagcttgttc
     4141 gacgcgctga cgctcctcgc gcgactccgg ggatcccgcg cgcgacccgg cgaggggcag
     4201 ggccggagcc ggtcgtactc gatcgatcca tacgaccccg gctggccctc gcgctacgag
     4261 gccgagtccg cgcgcgttcg ggaagcgctt gggaagcagc tcgttcggat cgagcacatc
     4321 ggatccacct cggtgcccgg ccttgccgcg aagccgatcg tcgatatcca agcgtcggtg
     4381 cgctcgatga tcccgcgcgc cgactacgtc gagccgcttc ggtcgctggg atatcgttgg
     4441 gtgatcgacc cctggagcga ccagcacgag tacttcagcc gcgatgacga cgggcggcgg
     4501 tcgttccaga tccacgtctg cctcgccggc agcgagtggg agcgtcggca catcgcgttc
     4561 cgcgactggc tccgcgaaca tcccggcgac gccgaagcat acgagcggct caagcgcgag
     4621 ctggcggatc ggcaccgtcg cgacatcctt tcgtacgtgg acgagaagac gccgttcatc
     4681 cggcagatcg agacccgagc cgccacccgc acccgctgag ccgtcgactt acgacccgtt
     4741 gtcgatcgtg acgaagaggc ccgattgggg cttcgggtcg aagtaggtgg ccttcgccgg
     4801 catctgctcg tgccggtcgg cgacctccac gaactggtcg aacgtaggcg gcaccagcgt
     4861 gaaggcagcg ccgccgtcct cgtcgacccg ggtcatgagc tcatcgaccg gcaccgcgtc
     4921 cgagacgaac tcgaggcccg gatcgccggc gtccccgaag ccgaagatcg gctccatcac
     4981 gaggcgatgg agccgcgaga cgtccaacga tccaacgccc gcggtcgcct cgtttccacc
     5041 gggtgtgagc cggtaccacc ggcctcggac gtagaggccg aagacgccgc gctcccctgg
     5101 ctgcgtggcc acctcctcca cggtcagacg ttcacggagt cgctgcagga gctcatcgac
     5161 gccgagcgga aggggtccca cgacccggcg gtggaagccc agcatgcgaa gctcctcgtc
     5221 cgggaacagg accaccggca cgccggcgcc gttcggtcgg ccggctcgtt cccagagcgc
     5281 gacggaggcc atggcccgat gatggccgtc cgtgatgtag agccggtggt cgtcgaggcg
     5341 ctcccggatc gtctcgaccg cgctcccgtc gtcgatccgc cagaccgtct gctcgagatc
     5401 gccggagcgg aatcgccgga gcggaatcgc ttcggtcgtg gcacgcacga tcttccgcac
     5461 gtcgtcgtcg gcgcggaaca tcagcgccac gagggtcgag cgagcgccga gccgggacag
     5521 atgcgtcccg agcgcatcca cgcgctcggg ctggacccct tcgtgcccga ggatgcgtcc
     5581 gtcgacgaag gcctggggag gcacctcgcc gacgatgccg gtctgctcgt gtccggcaga
     5641 gcggaggcga tagacgtaga gggcgggtac cgggaatccc tcgaagaggt ctgcatcgag
     5701 cagcctccgc agggccgcga cgttcgcctc gtccagcgcg gccgggtcga cgtcctggcc
     5761 ctccggcgcg tcatcgggcg tccgcgacac gtgcagccag gcgtacggac ggtcggcgag
     5821 gatctcggct cgctcttcgc gcgagagcgc atcgtgcatc ggcgacacga cgtgctccgc
     5881 ccattcgggc cggacgatcc gggtggggaa cgggacgagg gtgctcatcc ggcggtatct
     5941 cggaacgccg ccatgaaccc gaccagggcg tcgacccctc cgtcggtcat cgcgttgtag
     6001 atgctcgccc ggatccctcc cacgctccga tggcccttga ggttccgcat cccgcgctcc
     6061 tcggctccca cgaggaacgc ggcgtcgagc tcgtccgacg gcgtccggaa cacgacgttc
     6121 atgtgcgatc gatgggacgg ctcgacgggc gagcgataga agccgtcgct ccggtcgatc
     6181 gcgtcgtaca cggcgcccga gcgacgggcc gcccgttcct ccatcccggc gacaccacct
     6241 cggtcccgca tccaccggag caccttcccc gtgacgtaga tcgcgaacat cggcggcgtg
     6301 ttcagcaggc tgtgcttgtc ggcgtgtgcg ccgagacgca ggtagctggg gaggtcggtg
     6361 cgcatctcct cgagggcggc ccttcgcacg accatcaccg cgaggcccgc gggtcccagg
     6421 ttcttctgcg ccccgccgta gacgaggtcg aaccgctccc atggaaccgg cctgctcagg
     6481 tagtcggacg acatgtcgcc gaccagcggg agatcgatgt cggggaacga cgacatccgg
     6541 atcccctcga tcgtttcgtt cgaggtgacg tgcaggtagc gggtgtccgg ccggatctcg
     6601 agctcgtcgt ccgacggcat ccgcgagaac gagatcggtt ccccgtccca cgccgcgtag
     6661 gcatcacccc atatggccgc gtccttcaag gcgcccttcc cccacgtccc ggatcggacg
     6721 tagcccgcgc gcgcgccgtc gccgagcagg ttcatcggca ccacggcgaa ctggagggtg
     6781 gcgccaccct gcaggaacag gatcgtgaag tcgtcgggca cggcggcgtt ctctcggaag
     6841 agcgtcagcg cctcctcgtg gacggcctcg tactccgcac tccgatggct catctcgatc
     6901 acggacatgc cggaaccctc gaagtcgagg agctcggcct gtacctcctc cagcacgtcg
     6961 agcggaagcg tgcaggggcc ggcgcagaag ttgtggatcc tcacgtcggc cggtggtccg
     7021 tgggaacggc ggacacgtgg atgacgtcgt ccagcccttc cagggcggcg atcaggtcag
     7081 gctctacgtc gccgacgacg tcgatcgtgg cgacggctgc gttccgtccc tcgaagaccc
     7141 ggttctccat ctgctcgacg ttcaggccgc ggcggcgaag gatgtcgaat acgcccgcga
     7201 gcacgcccac acggtcgagg tgtcgcacgt ggagcgtgtg ggtcccgagg cgtgtggggg
     7261 caaggttcac gcagttgagg atctcgcctc tgacgtacga ctcgatgatc tccacgaccc
     7321 cgtcggctac ggccttctgg gcttgggccg tcgacgctcc gatgtgatgc gtcccgacca
     7381 cgcgggggtg ttgcgcgagc ggtgacgccc acgaggcgga gcccgatccg ggctcacccg
     7441 gatagacatc gagacccgct cggagctcgc gggtctccag ggcatcgagc agcgcggcct
     7501 cgtcgacgat gttcccacgc gacgtgttca gcaggatcgc gccctccttc atggctccca
     7561 ggagttgggc atcgaacatc gatcccgtct ccggcgagga gggcacgtgg atcgtcacca
     7621 cgtccgaggt ccccacgagc tcctcgagcg agccgacgag ctcgatctcg agcgacttga
     7681 tccgctcttc ggcctcgtcg tcccgatcct tacggaccgc ccggagggcc ataccgaacg
     7741 cgcccgcgcg ttcggcgacc gcgaacccga tctcgccgag cccgacgatg cccatgacct
     7801 ttccgaacag gccatccgcc ttcccgtagg tggatttgtt ccactcaccg gcgcgcagat
     7861 cggcgacgtt gtccgggatc cggcggtcga tcgcgaggag gagccccatc gtgagctcgg
     7921 ccacggcgat cgcgttacgg cccggcacgt tcgtcacgta gatcccgacc tcggacgctg
     7981 cttcgacgtc gatcgtgttg gtcccggctc cggcccgcac gatcagctcc aaggcgttcc
     8041 ccgcctcgac ggccgccgcc gtcaccttcg tactcctcac gaccaggacc tggaaacccg
     8101 gcatccgccc gggcaggtcg tcggaggaga gcttcggctc gaggacgcag tcgtgcccgc
     8161 gctcggtgag gacctccacg gtcgaggggt ggatcgcgtc ggcgaggagc accttcatgc
     8221 ctgggagcta agcagaacgg gcggatgggg aacaagccgg agcccctgcg ggggaagcgt
     8281 ggggcccacg ggtgtcgggc gacgtgcggc gaccttgcct ggcgcttccg cgccctcgat
     8341 gctatacaga ctccctcgac tgaccgggcg ttcaaggtcc ggccggaggg tgcgttgcgg
     8401 tccggatgga acccggaacg agacgaagtc caggctcgag ctgagcagag atccaccttg
     8461 gaagggggct gcgatgaagg gactgcgtcg ggcgtcgctc acgcttgcgg tggcaacggc
     8521 ggtcatggtc gggttggccg gatcggccct cggacaatcc gccaccgccg aggacgagaa
     8581 gatcactttc aacgtcggca ccacgagcga catggtctcc gccaacccgt tcaaggcgtg
     8641 ctgcagctcc gagtacgaga tgctgctgct gaactacaac atgttgtacg gcttctccgc
     8701 cgaggatctc tcaccggtgc ccgagctggt cgaggagcca gggtgcgagc ccagcgagga
     8761 ctacatgacc tggacgtgcc agatccggga cgacgtcaca tggcacgacg gcgaaccgct
     8821 caccgcggcc gatatcgcgt tcacctaccg cttcatcctg gacaacggac tcactacgtt
     8881 ctcggactat ctccccttca accccacgtt cgaagcgacc gatgacaacc agctgatctg
     8941 gacgtcggag gagccgacgc tcgctccgac ggtgcctccg tacatcccga tcctccccga
     9001 gcacgtctgg ggaccgctcg acggccagcc cgcgaaggag atccgtgcct tcgaggagat
     9061 ccccgcgatc gggtccggac cgttccagct cacggaatgg gagaccggtc agttcttccg
     9121 catggaggcc gtcgagggac acttcttcgg ggatccgacg atcgacgaga tcgtctacac
     9181 ggtcttcggg aacgacgagg ccatggtcca ggccctgaag gccggggaca tcgactacgc
     9241 ctacgacctc ccgcccacgc tcgtcgacag cctcgagagc gaagagaaca tcgcgatcgt
     9301 cgaggaggcg gccgactacc acacgaacct ggccttcaac ttcggaggac aggcggaggc
     9361 gcacccgaag gtgtacggca caccgcccgc gacccaggag acgaaccacc cggccttgca
     9421 cgatcacgcg gtccgcttgg cgatcgcgca cgcgatcgac aaggaagcgc tggcggaggt
     9481 cgtcttccag ggcgccgcac aaccggcgga cacgttcatc tcacccgaca aagctttctg
     9541 gcacctcgac atccccgccg aggaggagta cgcgttcgac ctcgaggagg cgaaccggat
     9601 cctcgacgag gcgggctaca cggaacgcac cgctgacggc gtccggatcg acccggagag
     9661 cggggaaccg ctcgtcctcg acatcgtggc gatcaacaac tcccgtgggt ccgcccgatc
     9721 cggcgagctg atggccggat ggatggagga gatcggcatc gagttcgatg tccgggtcgt
     9781 gagcgagagc aaggcatacg tcgagtggga gaacgggacg ttcgacgcct acatctggag
     9841 ctggggcggg gacccggatc cggacttcaa catgtcgatc tacacgtccg accagtgcct
     9901 cggatggagc gacggctgct acgtgaacga ggagctcgac cagctctacg ccgagcagcg
     9961 gcagacgttc gatcgagagg cgcgtcagga gctcgtccat gagttccaac ggatccacta
    10021 cgaggagatc cccgagatcg cgctcgtgta tccggagctc ctgcacgcgt accggacgga
    10081 cacgttcgag ggctacgtga actcaccgac gaacggaggc gcgccgatct tcgcttggcg
    10141 ggtggactcc tacatgaacc tccagcccgt gacggcggga gggcccggag gcaacgtcgc
    10201 cgatgaagga ggcatgtcca ccggtctcct cgtcgcgatc ggcgcgatcg tcgtgatcgc
    10261 gatcggggcc ttcgtggtga tgggccgcag gcgcagcgag gaggacgagg cgtaggttcg
    10321 gccgagcggg ccgagaaggc ggaggtcagg ggttgggatc gcggcggtac ctgctccgtc
    10381 atgtgctgca cgcgctcgcg accctggcct tcgtcttggc gttcaacttc gtcctgttcc
    10441 gcgcgatcgg ggatcccgtc aagctcctga cccgaagcag tctgcacctc gatccgcagg
    10501 aacaagaggc cctccgccag gagttcggga tcgacgatcc gcttcccgcc cagttcgtga
    10561 actacctcgg cgacaccttg cgcggtgagt tcgggtattc gttcatctcc gggcggccgg
    10621 tcatcaggtc ggtcgccgag cgggtctggc caacggtcgt gctcgtcggg accgcgacga
    10681 tcctgtcgac gttcttcggc gtgctgatcg gtatcaaggg tgcctggcaa cgtggaagca
    10741 ccttcgatac cggaagcttg cttgggtccc tcacgctgta ctcgatgccg gagggctggc
    10801 tcggcatgat gctgctgatc ctgttcaccg ggacgctcgg cgtgcttccg gcaggggggt
    10861 accagtcgag cgcgcagctc tcgggattcg ggcacttcgc cgatgtgggg aaacatctga
    10921 tcctgccatg cctgaccctc acgctcgggt acatcgggga gttcgcgatc atcatgcggt
    10981 cctcgctcct cgaggtgatg ggcgaggatt tcgttcaaac cgctcgcgcg aaaggcgtac
    11041 gcgacaagga cgtgcgccgc cgacatgccg tgccgaacgc cctgctcccc acgttcaccc
    11101 tcgcgttcta cagcgtgggc ttcatcctcg gaggtgccgt catcatcgaa gaggtgttct
    11161 cgtggccggg cctgggccag ctcacctacg ccgcgatcga ggcgaacgac taccccgtga
    11221 tccaggcggt cttcctcctg tccagcgccg ccgtgatcct gttcaacctg ctcgcggaca
    11281 tcctctacgg ctacctggac ccgagggtga aggagggatg acgtgatcga gccacgccag
    11341 ccctccgagg tcgccgtggg gaccgaggtt gccgctcccc ctccctcaag ggacccgaag
    11401 aagatccggc gggcccgccg acgccgggcg ttctccgggt tctggagggt ctaccgcgcg
    11461 agccggatgg ggatggtcgg actggtgctc ctggggatct tcgtgctcat ggcgttcacc
    11521 gcgccgctca tcgtctccga cgaggagctc agcgtgtcga ctccctcggg gggcccgttg
    11581 gagcctccgt cggcggagtt cccgctcggg acggacgact tcgggcgttc cgtcctcgat
    11641 ctcgtgatct acggctcgcg catctcgctc ctcgtgggat tcgctgcgac cgtgatgacg
    11701 atgaccgtcg gagccgccgt cgggatcacc ggcggctact tcggaggtcg cgtcgacagc
    11761 atcatgaacg cgttcacgaa ctggttcctc gtgatcccgt ggatcccgct cgcgatcgtc
    11821 ctcgcgtcgg tcctcggcag atccttgttc aacacgatct tggtcatcgg catcacctca
    11881 tgggccggca cggcccggtt ggtccgggcg gacacgctct cggtcaagga acgaccgtat
    11941 gtcgagcgcg cgcgagcgct gggcgcgagc aactggcacc tggtgacccg gcacatcctg
    12001 ccaaacgtga tgccggtgat cttcgcgaat acagtgctca cggtcgccct ctcgatcctg
    12061 gcggagacga cgctcgccct gctcggcctc ggagacccct tcagcacgtc ctggggtgtg
    12121 atcatcttcg atgccttcga gcaaggggcg ctctccgccg ggaaatggtg gtggctgatc
    12181 cccccgggcg tgtgcatctg tctcgtgacc ctggcgttca cgatgtgcgg gtacgccctg
    12241 gacgaggttc tgaacccgag gatcagggag cggtgatcgg gtgagcgtcc tctcgacgcg
    12301 cgatctccgg gtcacgtatc gcacgaaggc cggcgacgtc ccggcggtcc gcggggtcga
    12361 cctgaacatc gatcgcggac aggtgctcgg actggcgggc gagtcgggct gcggcaagtc
    12421 gacgatcggc ggagcgatcc tccggctcca cgcgccgaag acacggatcg aggggagcat
    12481 cctcctcgac gggaacgacg tgctctcgct caagcccgga cggctccgag ccgtccgctg
    12541 gaccggcgcg tcgatcatct tccagggtgc gctgcacgcg atgaaccccg tccgacggat
    12601 cggggaacag gtcgccgaag cgatcaccgt gcacaagctc gccggcgagc gggaagcgag
    12661 ggtccgcacg gccgagctcc tcgaacaggt gggactgccc ggcaggcgca tcgacgacta
    12721 cccgcacgag ctctcgggag ggcagaagca gcgcgtgatg atcgcgatgg cgctcgcctg
    12781 caacccctcg ctcgtgatcg ccgacgaacc caccacggcg ctcgacgtga tggtgcaggc
    12841 gcaggtcctg aagctcctca aggcgctcca gcgagagctc ggcctcgcga tgctgttcat
    12901 cacgcacgac ctctccgtgc tcgtcgaggt cgccgatcgc atcgcgatca tgtacgccgg
    12961 cctgatcgtc gaggagggac cggcagagcg cgtcttccgc tccccgcagc acccgtacac
    13021 gaaagccctg gcgggggcgt tcccggagat cggcgacaca cagttccggc gcaaaccctc
    13081 tggtctcgga ggcgatccgc ctgatccgca gcagatcccg tcggggtgcc ccttccatcc
    13141 tcgatgctcc gaggccatcg acgtttgtcc ctccctcgat gtcgatctgt gggccgcggg
    13201 cgagggccgg cgtgcggcat gcatccacgt ggaaggggcg ccgtccccgg cggaggcgcg
    13261 atcgtgaccg gaacggagac gggcgtccga accgccgtca tgggtgagcc catcatcgag
    13321 gtccgagacg tggaggtcag cttcaccggg cgactctcgc tactgaaggg cttgggaggc
    13381 ggcaagggga cgatcgccag ggccgtcgac ggtgtcgaac tcacgctgca cgagggcgag
    13441 gtgcttgccc tcgccggtga gtcgggatgc gggaagacga ccctcgcgag ggcgatcatg
    13501 ggtctgatcc gtccgaaggc gggcgagatc tcgttccgcg gcgtcaggct cgggcggagc
    13561 ctgcgcgagt atcggcgcga ggtccagatg atcttccagg atccgaccgc gtcgctcaac
    13621 cctcggcaga cgatctacga gagcgtggcg gagggcctcc gcatccacaa gatccatcaa
    13681 ggaccggacg gggagacgga ggagcagctc gtcgcccagg cgctctcgcg agcggggatg
    13741 cggccgcccg agcgcttcta cctcctgtac ccccacgagc tctcgggtgg ccagcggcaa
    13801 cgggtcgtca tcgcgggtgc gctggtgctc gacccgaagg tgatcgtcgc ggacgagccc
    13861 gtctcgaacc tggacgcatc ggtgcgagga gagatcctgc agctcctgct caagctccgc
    13921 gacgagctcc gactgtcgat cctgatcgtc acgcacgacc tcggtctcgc gtggaccgtg
    13981 gcggaccgtg tggcggtgat gtacctgggc aggatcgtcg agatcggccc cgcggaggaa
    14041 gtgctcctca gacctcggca cccgtacacg aaggcgttgc tcgacgtcgt tccggaggcc
    14101 ggcgggatag atcgtccgat cctctccggt gagccgcccg acccgacgcg ggtgcctccc
    14161 ggctgccggt tccatccgcg atgcccggtc gtcgcgtcgg gtcgggcggc gacgctcggc
    14221 atcgagcagc ggtgcaaggg agaggacctc ggtctcgagg agctcgctcc aggacatctc
    14281 gccgcctgtt acgaggcgga gctcaccgtc gctagcaggt gacagtctcg ctatgcccct
    14341 gcaccctcat cggcctccga cggaacgggc cgttcctcgg gggcggcttg atcgccattg
    14401 cccttgagca caccgaggat cgcctccatc tccgagtcag gaagacgctg gtcgggatgc
    14461 atccccgtcg cacgcgcgga ggcgagcagc gtcctcaggt tcgactcggc catctccgcc
    14521 ctgtgctcca gctcccgagc ccgctcggcg aacgtccgcg cccgtccctc cgcctccacc
    14581 cgcatctccc ggctctcgcg agactcttcg cgtgacgtct tgagctgagc cctgagctgc
    14641 gacacctcct gactcagctc caccagccgg tccacggtcg cccggcccat gtcggagtac
    14701 gccagcccgc cgctcctccg gcccggctgg gcatcttccg accgcttctt ggtccgccgc
    14761 cgcttgcgct cgatcgtctc tcgctctccg gccgccacgg gcgtgtcatc caacagccgg
    14821 tacgaccccc cggccggctc gagtcggcgc cgagcgacga gcgccctgac tgcgcgcgcg
    14881 atcgacaatg gatccgggaa gcccaatccc ctccccagct cagctggcgt ggcttccagc
    14941 agttcgccct tctgggtgcg ctgggcaact gcggcgagga acgcattgac gcgctcgagg
    15001 ctctgctcgt tcacagctgg attctgcctg attgtcgact cgctgcgcca gttgtgccga
    15061 agggcaccac gccgggcgga taggctcggc gtcggtggac gatctcccgc tctccggctg
    15121 tcgcatcctg gacctctcac gggtgctcgc cggaccgctg gcgacccaga tcctgggtga
    15181 cctcggcgcg gacgtcatca agatcgaacg cccggaacag ggcgacgaca cgcgccattg
    15241 gggaccgccg ttcgtggggg acgatgccgc gtactacctg gcgctcaatc ggaacaagcg
    15301 gtccgtcgca ttggacctgc tgtcgcacga tggcgcggac gccatccgca gcctcgccgc
    15361 aagctccgac gtgctgatcg agaacttccg accggggctg atgagcgagt tcgggctggc
    15421 gctcgacgac ctccgccggg cctcacctcg gctgatcacg tgctcactga cggcgttcaa
    15481 cgacgagatc cccgagagcg cgagccgccc cgggtacgac atcatcgtcc aggcgctgtc
    15541 cgggctgatg agcgtgaccg gtgagcccga cggggagccc gtgaaggtgg gggttgccct
    15601 cctcgacgtg atcacgggat tggagatggc gatcggcatc ctcgcagcgc tccacgagcg
    15661 ttcgcgaacg ggtcgggggc ggcacatcag cgtgtcgctg ttcgacgccg gcgtcgccgc
    15721 catggtcaac caggccgcca accacctcct cggcggcgtg gtcgcgcgac cgatgggcac
    15781 ggcccacccc aacatctgcc cctaccaggc gttccacgcg tcggatcgtc cgttcatcgt
    15841 ggcggccgcc aacgatcgac tgttccggcg gacgtgcgag gtcgtcgggc acccggagtg
    15901 ggccgtcgat ccccgcttcg cgacgaacga tgatcgggtg cggcatcgag acgagctgat
    15961 cccgctcctc gccgagtcgt tcggagggaa gaccgccgcc gaatgggtcg cggccctcga
    16021 ggccgcgtcc gtccccgcgg ctccgatccg ggcgatcgac gaggtgttct cctcgcccga
    16081 gggagccgcg atggtcgagg aggtcgcgga cccgggtcgc gggacgacgc tgcggctgtt
    16141 gcgcaacccg atccgcttcg atggggcggg gctggacaca cggctgcctc ccccgctcct
    16201 cggcgagcac acggacgagg tcttgcgcga gcggtgaagg ccgtcgagcg ctggcgcgag
    16261 cggctgggac gctgggcgat ccctcccgag atcctcgctg cggcgcccga gtctccgtgg
    16321 gggttcccga ccgagctgtt ccgcgcccga ggtgcgcggg gtgccgaccg agagggcacg
    16381 acaccgacga cggcgagagc gctcgagcga ctccccgagg ccgggagggt gctcgacgtg
    16441 gggtgcggag gcggcgccac ctccctcccg ctcgccggcc gggcgggcac actcgtgggc
    16501 gtggacgcac aggaggacat gctcgacggc ttcctcgcga acgcccgcgc ggccggtgtc
    16561 tcggccgtcg cggtcctcgg gcgctggccc gaggtcgcgg agacgatcga cccggtggac
    16621 gtcgccgtcg ccggacatgt gctctacaac gtcgccgacc tggagccgtt cgtcctccgg
    16681 ctcgccgctc tcgcacatca tcgggtcgtc ttcgagctca cggatcgaca cccgctcgcg
    16741 tggatgaacg acctctggcg ccacttccac ggcctcgagc ggccgaccgg ccccagctcg
    16801 cacgacgtcc tggacgtgct gggcgagatc ggcctcgctg cgacgctgga gcggtgggaa
    16861 gccccgccgc cggtgggcgg gttcgccgag cgagccgacg cgctcgcgct cgcccggcgg
    16921 cgactctgcc tgccctcctc ccgcgacgac gagctcgcct cggccctcgg ccctcggctc
    16981 gcggaacgag gagggctctg gagcgtcggg ccgcccgggt ctcagcgcct cgcgacgatc
    17041 tggttcgacc cctagccgcc gaggcggatc gtgatggccg accggtagat ctcgtcctcc
    17101 ggatcctcga tcctcgtctg cttcacgacc tcgctgccgg ccgcctcgac gatcgccact
    17161 cgcagcgtct ccaacgtctt gacgaacgcg cccaggtcct gggatccggc cggcatccgt
    17221 cccacgtctc ggaaccggag gtcgagctcg tccccgtccc ggcgaccgag gaacaggacc
    17281 aggatccgct cggcctgcac ctgcgccgag gggaagtcgc aacccgcccc cggctggagc
    17341 cgcgcgacgc cacggcccgt gatccgtccg gaggcggaga tccggagcgt gcccttgacg
    17401 cgccacgcgc tcgtgcacga cccccccacg tggaacacgt gcgtcgtccg gctcaccatc
    17461 gtgatcggcc actcgtcgtg ctccggaggt gcagggctcg ggccgtcact cggttgggcg
    17521 cctgggtcgg ggaccccggg agggaacggg ttcgagcgcg acccgatgaa cgcgccgacg
    17581 ccggcgcagg ccaagaggat caccacccac aacaggaccc tcgcccatcc acgcacgtgc
    17641 caatcatctc gcagcccgac gggtgcgccg ctacaggccg aggcccgtca tcagtgcccg
    17701 gagcgtgagc ccgaagctga tgaacgacgc ccacgcgaac ggcacccatg gccggagggg
    17761 ccgatcgatc ccgaccgcgc gcgcatcgcc ggcccgggcg acgacgtggg cgaacacgaa
    17821 caggagcagc gggagatagc cgtagacgta gtggagcgcg ccgcccagcg ctccctcggc
    17881 ttcgagccga tggccgagca gcagcaggat cgtgccgacg atcgcctgga cacccgccac
    17941 cacctgggcg atggcgagcc acgtccagta cccctcggac ggatcgcggc gcgcgatcct
    18001 cgctccgagc ccccagatcc acccgagcgc gaagatggcg acgaccgcga acccgatcac
    18061 gcgatgcagc gccgtcacgg cgcggatact acggaaccgg cgaggaccgc ttcgcgatca
    18121 cgaacgcgac cgccaacacc ggcatggcga tcgacagcag ctgcagggct gcttccggcg
    18181 ccctccggat cgcctcgggt ccgatcggtt ccgtggtgcc gtcccggagc gtgatcgtcc
    18241 cctcgaccga ggcgacccgt cgccggagcc ggcggaccgg cgtggagagc gcccgctgcg
    18301 ccgcgctgat cgccacgcag gcggccgcgg ccgcgaccgc cgcgagaccc agccgcccgt
    18361 cttgtgcgaa cgccccggtc agggcgggga agcctcccca ggcgagggcg aaccacagat
    18421 ccgagtggaa ggcgccgccc aagagctcga ggttgtaggc gacgaccagg aacgctccca
    18481 ccacgatgaa caacgcgagc cacccggtca cttgcacgat cccgatcgtg cccaccgcag
    18541 cggcccccgc gagcgagacg gccgcgaccg cgacgagcac gccgtccggt atcgatgtct
    18601 cgagaggcct gccccggagc tcgtcgagcg cgtgcgccgc caggcccacc ccgaggaaga
    18661 atccgaggag gctcagcccg agccggtaga ggttcagctc cgccgcggtc gacgccccga
    18721 gcgcgacgta ggagaggtgc cagagcgtgt agggcgggtg caggagggtc caccagtcgc
    18781 gccaaccccc cggtgcgagg gcgtagaacg ccggccgatc ctcgctcacg aggacttcac
    18841 gccccagagc accacggcgg atccgaacag gaacgacctg gaccggacat ggcggatccc
    18901 ggcggcctgg aaccaacgga cccaatcctc gatcggatga tcggccacga atcgagagat
    18961 gttcggtccg aggaacgccg tggtccgcgc ccacgcgggc gagaccgcga cgccgaggag
    19021 ccgcatcacg acccgggtct ggacccacca gccgagacga gcgagcggag cctccgggac
    19081 ggagaactcg agcatcgcga tcgaaccgcc cgcccgcagc acccgcgcca gctcgcgcac
    19141 ggtcgcggcg gggtcctcca cgtaccgcaa cagataggtg aaggacactg cgtcgaacga
    19201 tccgtcctcg aacgggagac gctccgcctg tccgagcacg ggccggatcc gcccgtccag
    19261 gtcggccagc cggttgggct cgccccccgc acggagcatc ggctcgctct ggtcgagcgc
    19321 cacgacgtgg acgttccggc ccgcggcgag cgcacgggac acgagctgcg tcccggcggc
    19381 gacgtcgagg acccgcccgc cgggcaccgc gttggacctc gacacgagga atcgacgcca
    19441 ccgagcatcc tgcccgaacg acaggagcgc ccccatccgc tcgtactgcg tggcgatacc
    19501 cgcgaagagc cgcttcgcga accgcgtgcg ttcgacggcc aacggatgcc gcctacgggg
    19561 atccgaggaa ggagggaacg tccgcgaacg agtccaagac gtgatcgggc cgtccaccgt
    19621 cacgtgcgtc gtctccggcc cggtacttcc ccgtgcggac caggatgccg gtgaggcccg
    19681 ccgcctgtgc gccgaggatg tcgttcacga tgtcgtcgcc gaccatcgcg gctcggtcgg
    19741 cctccacgcc gagcatcgcc agcccggcgt cgaagtacgc cttttccggc ttcccacaga
    19801 tcgcggcgcg cgtgtccgcg gcctcctcga gccccgcgag gtaggccccg ccgtccagct
    19861 cccatccgtc cgcggtcttc cagaacaggt tccgatgcat ccccacgaga gccgcgccgt
    19921 ccatcagccg acggaagatc cggttcatcg tgtcgtacgt gaactcctcg cacgcgccgc
    19981 cgatcgcgat cacatcggca tcgtcgacgt ccacgaggtc gatcccgacg aggtccgccg
    20041 tcgcgtcccc gtcggatagg acgaagaccg gggcaccggg gtgcgccgtt cgcagatgct
    20101 ccgccgtcgc gaccaccgcg gtcacgacct cctcgtccgc gacgtcgaac ccggcgtccc
    20161 gcagcgtcgc cgccaggttc gcacggctct tcgtggtggt gttcgtgatc agccggaacg
    20221 gcaccttctc gtcacggagc cgagccagcg catccaccgc tcccggcagc gcttcccacg
    20281 agaccgcgag gacgccgtcg atgtcgagca acaatccgtc gaacgtcacg gacaggagtc
    20341 tgccatcacg gacggatccg gagcgaacgc gcgggttcgg gctcgcccgg agcgccgagc
    20401 cgacgccggc gacgccagga ctgatacgga gcgatgtagc ggaacctcgc cgggagcaac
    20461 ggccgaccgg cccgcatcgc ggcgaagctg gcgcgacgga tcgctcgctc ccccttccgg
    20521 tgtgggagct gatagccggc tcggacctcg gcgggcaacg ttccgaaggc gagccgcgcc
    20581 acgaggcgaa ggacgggtcg ccactgggcg tcgggcggtg gatccaggaa gaggccggcg
    20641 acgctccgcg ccgcgtccgt cacgaggagc tcccctgagc gaaccgtgtc ggcgacgtac
    20701 tcctccaact cgcgcacggt cggcgggatc aggtcccgag ggaggcggac cagctcggca
    20761 gcgagcatct gctcctcgtg gaaccgctgt ctcccgcgat cgtcgagccg cccgacggtg
    20821 agccgttcga acaggagggc gctctcgacc agacaggcgt gcacccagag cagcagagcc
    20881 gggtccgtcg cgtcgtaccg gcgtcccgtc accggatcga cgccgttgat ccgctcgtga
    20941 acgtcgttga tgcgatccgc cgcggcgtgc gcttcctctc tggtaccgaa ggtgagggcg
    21001 tactggagca cgagcgttcg ctgcagtcgc ttccacgggt tccgctcgta catccctgtc
    21061 tggttcgcgc ctgcgatcac ggacggatgg gccgcctgca tcagcatcgc cctcgcaccg
    21121 ccgaacagga ccgtgacctc tcgatgcacc tgccaggaga cgctccgcgg cccgaagtac
    21181 ccttcgtccc gatccatgga ccaaagggta gcgcggtcct ccggctggac tcccccggag
    21241 gctcgtggaa cgattggccg agcacccgta cgagtcggag acttccccac gtgctgcttg
    21301 ttctgatcgt gatcggcttg gccgtctgcg tcgggttcgc cgccggcggc agcctccggc
    21361 cattcgagcg cttccgggtg cattggtggg gcgtcgccct cgtgggtctc gccctccaag
    21421 ggatcccatt cacgagtggt gtcagccgac cgatcggcac cgccgcgctc ttgggctcct
    21481 acgtgctact gggctccttc gccttcgtga accgtcggtt gccggccgtg tggctcgtga
    21541 tggtcggcct cgcactcaac ttcgtggtga tcggggtgaa caacgggatg ccggtgagcg
    21601 caggggccct cgaggtctca ggcgcgagcg ccgaaggtct cgccggcgag ggcacgctca
    21661 agcaccacct catgggcccc ggcgactcgc tgacgccgct cggcgacgtg atcgggatcc
    21721 caccgcccgt cggcgtcgtg atctcgatcg gcgacgtgat cctctacgcg ggagtagcga
    21781 tcctcgtggt agccgtcatg ctcggacggt cgggtgagaa tcggaggccg ccggctcgct
    21841 ggttccaggg atatcggggg aagcacctcc gtccacgtcc ccggttctcc cgtctgtagg
    21901 gccagcgggc agcggtttgg cgccaccgct cccgtcatgg ggatccgggg gagcctgggg
    21961 agccggggga ggctcgtccc caaggagcga cttggggaac ctgacgatga tggtcgttcc
    22021 ctgacccggc gtgctttgca cctcgaagcg tcctccaccg acctgcgcgc gctcacgcat
    22081 catcgccatc ccgaagtgtc cttccggacc cggcgcttca gtgtcgaagc ccactccgtt
    22141 gtccctgagg ctgaggacga tgtcggcatc ctcttcacgc acaccgatcc acacctctct
    22201 cggttgcgcg tgcttcatcg cgttcatcac gccttctcgg gcgatgtgga acacgagcaa
    22261 cgcgatcggc gccggcagct ggatgtccgc gacatcccgg tggaaacgga tcgaagaatc
    22321 ccggccgacc tcgtcggtga acgactcgag ggcttccgcg agacccttcg cccccagcgg
    22381 ggatctatgg aggtctcgga tcagcgcgcg gatccgatcc gacgtctcct gcttcgattg
    22441 gcggatcttg tcgagttgcg cgcccacgtc gtcggtctgg cctttctcga gcagcttgcg
    22501 ggccacgtcg acctggatcg acaggcggaa gagcacctgc gcgagatcgt catggaggta
    22561 gcccgcgatc tggaggcgtt catctgcacg ctcctccgcc atcgcgttcg agagagcacg
    22621 gaggacttgc tcgcgctcct gcagttcctt gtgggcttcc tcgagtgctt gcgaacggaa
    22681 gaacatctgc cgagcaaaca agagaggcgc caggattgcc ggcagagcga agaagtcagc
    22741 agcgttgtag aggaaagcca agctcagacc caccaggcca agaccgaggt agctgatcaa
    22801 gaactcactc aatcgcccaa tccgcagttc tttcatgact tggaggatgg gcacgccggt
    22861 tcggagtgag acaaacatcg tgacgagtcc gctgttgact gcatagtcga ctagcgaagc
    22921 ggatgcagcc gcgacgagta agaggaccgc agatgtatgg gaggtgcccg cagcgaacgg
    22981 ggtcatccaa tggaagaccg cgctcgctgc aagaacggac agggcaactt ggcagcgatt
    23041 gaaaagggct cgcaggacgg taacttcacg ctggaactcg cgagggtcag atgcacccac
    23101 caaagcgact gcggcggcgg cggcgggctg atagagaaag gccacgacca tcatgagcgg
    23161 gaagccgacg ctaatcgtca gaccacgcca cgctggcacc ggcaggagct cgacgaggcc
    23221 aatgacgatc gcccatacga ccagtggcca gccatcctca aacgcggatg gatcccgaga
    23281 aatcaagaag gcccccaagg cgaatgtagg caggatgatt gacagctcga aggccatcac
    23341 cctccccggg tgcacccggc ggccggatac cgtcatggac gtcaatcttg gctatccaag
    23401 tcccaagcgc aagctcccaa tgaggcacct acagcaagtg tggtatgggt cccctacagc
    23461 atccttcgta gctgcattgg cgtgcttgta agtcccccgt tctacgccaa tattcgccta
    23521 cggcatcccc caaatggggg gtggccatag gtccaaaggc gggactgctt cgttgggccc
    23581 gttggatgag agggggatcg gcatgaaggt cgtgcgacgg atcgcaatcg cgctcggctc
    23641 actgctggct gtggctctgg cgggcggcgc tcactggcga ggctaggcta gacactgact
    23701 ggttcgaggg gcccctcggg gccccttctt catctctcag gcgttcttcg gaatactggc
    23761 ggctaccaga gttcctacac caaggcgaga gtcgacaaac atgctgccac ctgctgcctc
    23821 aaaccgctcc ttcattatct ggagcccaaa gtgacggctg ccatcgaccg cggtccatga
    23881 gaaccccgtc ccatcatcgg taacactcaa gcggagcaac gcctcgtctg tccacaccct
    23941 tacccctatg ctggatgctt gggcgtgttt cgcagcgttg tgcagagcct cccgaattgc
    24001 ctgatacgca agcagctggg cgagtggcga actgtcgagc tgttccacgt cgagggttac
    24061 gggcggcgct ccagctgacg ccagctgatc taccaacagc cgcagtgtcg gaatcagccc
    24121 ggaagctccg aggggcgacc ttcgcaggtc tccgagcaaa tctcgaatgg ctctttgcgc
    24181 agcgtccgtt gcgaagagga gctcgggcaa gtcatcatcg agctcgagga gcttcccggc
    24241 cgcgaggtct tgccttagaa cctgacccat taggtggacc ttgaataggg atgggagaac
    24301 ctcgtcgtga agctctccag cgagtgctag gcgttcatcc ttccgttcgc gaaccacctc
    24361 atcggcagca cctaggatgg cgacgtcctt aaggcgaatc cgttccgtgg cgtcgaggaa
    24421 ctgcttagtc tgccggaaca tctcgcgagc caagacgagt ggcgctagaa acagcaagac
    24481 tgcccagatt ccgccagtgg cgaaagctgc cgacagaggc atcgcgagaa ggccgaggag
    24541 caggtagccg gcgaggtagt gccatggcga tgctccaaac atcctgagca agacgcctcg
    24601 aggagcaagg cgatcctcta ttgctaccgc agacgcaaca agaaggaagt tgacccctgc
    24661 atcaaacagt agcgccacca cccccggaac caggacacct ggccactccg tgatcgttcc
    24721 gcccaacgca tggaaggcga cagcccctgc catcacgctc gcggcaactt gactgcgatt
    24781 gaagatcgac ttggctagcg agacatgcct cgacagctct cgtgggtcga aggaaccaac
    24841 aaaggcaatc agcgcggctt ccgccggaga gaagatcatg ccggcagcca atgccactgg
    24901 gaaggacatg ctcactgtga cggatcccca gacgcgaaca ggaatcatgt ccaccgccgc
    24961 aacgatcgct gcccatgagg ccagctgaac caagccggta gggtcgaggc tctcccgaag
    25021 agcgaatgaa atggtggctg ccactgacag gaaactcact acccacaaga aggcagttgc
    25081 acggcgtggc ttacgttcag ccgcggccgg gtcccttcct cctcctcggc gactcgcgta
    25141 gaccgacatc tgccctcctt gggacgaagc ggtctccccg gtcaccgcgg gaacgaagac
    25201 ctgccgattt gtagcccatc tccggtgtct cgttcaagag atgtctcgtt cgagagtgac
    25261 aatcccggtc gggagccagt cacggctcac cgtgcgaaac tcgatgccat gaccaccgaa
    25321 cgtgatccgg atcggaggac gcgccggaac ccattccggg acgaggtttg gggcgacgag
    25381 cctcaggagg tcgatgcctc ggagttcgtc gaggaactcg agaccagccg cccggatctc
    25441 gcacgccggt ggcacgacca cccgaccatc gttcccttca aggttgtcgg tcgattcatc
    25501 ggtcggaaca gcaagcgtgt cgcggtcacg atcgccggct tcgtcgtagt cctcgctggt
    25561 gccgcgatgc tcgtgctccc cgggccgggg ctgctcgtga t
//
LOCUS       DASUYD010000100         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_713629, whole genome shotgun sequence.
ACCESSION   DASUYD010000100 DASUYD010000000
VERSION     DASUYD010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_713629"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..441)
                     /locus_tag="VF195_05540"
     CDS             complement(<1..441)
                     /locus_tag="VF195_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580318.1"
                     /translation="MRTRRGRGRKRATAAWRRARTIALILGLAAALGAPSTLVASRGS
                     APRAQVIVMAEPGATASIESAIEHVGGVVERRLSIIGGFSAAVPADALLTVRGLPGVV
                     SVSADSEMQPQDAAYDPATDVDSMNAVTRYTGAVDWWNTGYTGAG"
     gene            complement(916..1179)
                     /locus_tag="VF195_05545"
     CDS             complement(916..1179)
                     /locus_tag="VF195_05545"
                     /inference="COORDINATES: protein motif:HMM:NF039408.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6326 family protein"
                     /protein_id="HEX6580319.1"
                     /translation="MSNDAIVEELRIGVRLKISALWIAILFLFAYGDILGFFQEGQIE
                     EVIAGEVSGIEITETFLFAVSVYIAIASLMVFLSLVLRSRVNR"
     gene            1617..2807
                     /locus_tag="VF195_05550"
     CDS             1617..2807
                     /locus_tag="VF195_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580320.1"
                     /translation="MTDRRMGIIDRYGRFVWGGAALLLLFVLIVSVTLSGSSVAGAEQ
                     DAQVRAESLSGSVVEEELTPDLLARDIGGTAYRDLTARVQAGILSDDRFTVVRIWRLD
                     GALIYSTAQRDDAGVIAPDDQWIAQALEGRTVSVLSSAGTYHDGLKRPIEEMFQTFVP
                     IRSSSDSLIDGVVQIDQRYSAIHNQAYRVWRPVQFIALVLLVAIGVLFVRWLRDAAVT
                     VEPAAQIERRVTPGRRADDVQVRDAVERADRAERLARESEKRLEELEAMVAAAPSTAT
                     ATAALEELDLKLRASEAEREELVATVHRLQATLAERDAEVALARDGSTGTRGDIKRVN
                     KLIADAESRAVMAERKAAAAEKKTQEAARRATVTAERALEMEAQLSEAEQRAARTETE
                     LAEA"
     gene            complement(2804..3379)
                     /locus_tag="VF195_05555"
     CDS             complement(2804..3379)
                     /locus_tag="VF195_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580321.1"
                     /translation="MASVRLETPVPRLIEPIQAWRTWTLVGSRDGTDVRLAPIAGDGK
                     PWPPRRPAAATCTRHRSHVGPELHCTCGFHAASSPDALRRTRDPAVLGTVCLWGRVVE
                     HEHGFRASFAYPQRLQLVCYLCFSLWGRRAPRDCDVVVRHRGGRMVPLCEPHLELSRR
                     YGYRVPRVLPGRPVRSALLATYAVDLLRELD"
     gene            complement(3383..3511)
                     /locus_tag="VF195_05560"
     CDS             complement(3383..3511)
                     /locus_tag="VF195_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580322.1"
                     /translation="MDIGRPQRIIEVEPVSLPLPDELPDLEPAPAEPTRSEPDPAR"
BASE COUNT          642 a         1280 c         1281 g          574 t
ORIGIN      
        1 gccggctccc gtgtagccgg tgttccacca gtcgaccgcg cccgtgtagc gggtcacggc
       61 gttcatcgag tcgacgtcgg tcgccggatc gtacgcggcg tcctggggct gcatctccga
      121 gtccgcgctg acggagacga caccgggaag gccgcgtacg gtcaggaggg cgtcggcggg
      181 aacagccgcc gagaacccgc cgatgatcga caggcgccgc tcgaccacgc cgccgacgtg
      241 ctcgatggcg ctctcgatcg aggctgtcgc acccggctcc gccatgacga tgacctgtgc
      301 gcgcggggcc gaaccccggg aggcgaccaa ggtgctcggc gcgccgagcg cggccgcgag
      361 ccccaggatc aatgcgatcg tgcgcgctcg acgccacgca gcggtggccc gcttccgtcc
      421 tctgccgcgc cgggtcctca cgtcgggagc cttcctcgac ctcgcacggg cgactccatc
      481 catcggtcgc cgacgtactc gggtagtgtc acctcaccga ccgaccgccg catcgacctt
      541 tcgggcccat cctgcggtag gaactgagtg gtcaccccgc tcgggcgggg gaagccgaaa
      601 ggcctagttg gaggtggccc tatggctgaa ggcagtcagc atcggccggg tggtcacggg
      661 accatgtttc gttgtcacgt caccctttcg agggtgaagc cggaacagct gaagaacatc
      721 cggtggtaac cgccctgccg gatgacgaga tggaggtcat gtgccttccc gcttaggcca
      781 tgtccatgcg taccagatga tcagcagcag gagtacgcct tccgtgaggc tcaggaagat
      841 gaaatacgcc gccgattcac cgataaccga aaccgcgatg gagacgatgt acaggatcgg
      901 caacacgatg ttggttcagc ggttgaccct cgaccgcagg acgagcgaca ggaataccat
      961 cagactcgcg atcgcgatgt agaccgagac tgcgaacagg aaggtctccg tgatctcgat
     1021 ccccgacacc tcgcctgcga tgacttcctc gatctgtccc tcttgaaaga agccgaggat
     1081 atccccgtac gcgaacagga acaggatcgc gatccacagc gccgagatct tgagccggac
     1141 accgatcctc aattcctcga cgatcgcgtc attggacatc gacaccttgg cccctcccct
     1201 ttccctcggc ggcccgtcgc ggaagaagcc taatcaccgt cggggcacca tgaggtgacg
     1261 tcggtcgaaa gccacttgcg ctccggttcg cgcgcccgga caatcagcag atgagccgct
     1321 tcacctgacg ctgatcgacc accgctcgtg gtcctccttg gccccgggcc cctccgggcc
     1381 cgaccggtcg cgtatcgatc gagatggccg cggttgcctt ccgtttcacc gctcgatgct
     1441 cattcggccg gaaccagcgt ccgggcctga tgacatcact cgtcgggtgg tgctcccatc
     1501 ccgcggtgag ctcatctcat cgaagcctca tccatcgcgt tcaagcctga aggccgcagg
     1561 gccgacaagg gggaggggac tgcccgacgc gtgagcgggc agccgttggg ctgcacatga
     1621 ccgatcggag aatggggatc atcgaccgct acggtcgctt cgtctggggc ggagccgccc
     1681 tgctcctgct gttcgtcctc atcgtgtcgg tcaccctctc gggatcctcc gtcgccggcg
     1741 ccgagcagga cgcgcaggta cgagccgaga gcctctcggg atcggtcgtc gaagaggagc
     1801 tcaccccgga cctgctcgcg agggacatcg gcggcaccgc ctatcgcgac ctcacggccc
     1861 gcgtccaggc cggcatcctg tccgacgacc ggttcacggt ggttcgcatc tggcgtctgg
     1921 acggcgccct catctactcg accgcgcaga gggacgacgc cggcgtcatc gcacccgatg
     1981 accagtggat cgcgcaggcc ctcgagggcc ggaccgtcag tgtgctctcg agcgccggca
     2041 cctaccacga cggcctcaag agaccgatcg aggagatgtt ccagaccttc gtccccatcc
     2101 gctcgtcgag cgactccctg atcgatggcg tggtgcagat cgaccagcgg tattcggcga
     2161 tccacaacca ggcgtaccgc gtgtggcgac ccgtgcagtt catcgcgctc gtcctcttgg
     2221 tcgcgatcgg ggtcctgttc gtcaggtggc tgcgggatgc tgcggtgacc gtcgagcctg
     2281 cggcccagat cgaacgccga gtgaccccgg gacgccgagc cgacgacgtg caggtgcgag
     2341 acgccgtgga gcgtgcggac cgtgccgagc gcctggccag agagtccgag aagcgattgg
     2401 aggagctcga ggccatggtg gcggccgcgc cgagcaccgc gacggcgacc gccgccctgg
     2461 aggagctcga cctgaagctc cgggcctcgg aggccgaacg cgaggagctc gtcgccacgg
     2521 ttcaccggct ccaggcgacg ctcgccgaac gagacgccga ggtcgccctg gcgcgggacg
     2581 gatccaccgg cacacgtggg gacatcaagc gggtgaacaa gctgatcgcg gacgcggaga
     2641 gccgggccgt catggcggag aggaaggctg ccgctgcaga gaagaagacc caggaggcgg
     2701 ccaggcgcgc gaccgtcacg gccgagcgcg cgctcgagat ggaagcccag ctcagcgagg
     2761 ccgagcagcg ggccgccaga acggagacgg agctcgccga ggcctaatcg agctcccgca
     2821 gcagatccac ggcgtatgtc gccaagagcg cggatcggac cggccgcccg ggcaggaccc
     2881 tcggcacccg gtacccgtac cggcggctga gctcgaggtg cggttcgcac agcgggacca
     2941 tgcgaccgcc gcgatgccga acgacgacat cacagtcccg cggcgctcgc cgcccccaca
     3001 acgagaagca caggtagcac accagttgca ggcgttgcgg gtacgcgaag gaagcgcgga
     3061 acccgtgctc gtgctccacg acccgacccc acaggcagac cgtcccgagg accgccgggt
     3121 cgcgggtccg cctgagcgcg tcggggctcg aggcggcatg gaagccgcac gtgcaatgga
     3181 gctcgggtcc gacgtgcgat cggtggcggg tgcaggtggc tgctgcgggc cgtctgggcg
     3241 gccagggctt gccgtcgccg gcgatcggag cgagccgaac atccgttccg tcccgagagc
     3301 ccaccagggt ccacgttcgc caggcctgga tcggttcgat cagtcgcggg accggggtct
     3361 cgagccggac ggatgccatc ggctatcggg ccggatcggg ttccgaacga gtgggctcgg
     3421 cgggcgccgg ttcgagatcg ggaagctcgt cgggcagagg gagcgagacg ggctcgacct
     3481 cgatgatccg ctggggtcga ccgatgtcca tgggtgcgct cctcgggtgt tcctcgaacc
     3541 gagttccggt cgcgagcgta ctcccgcggt ccgacgtctg gccagcgcct cgcgaaacct
     3601 caggcgcgga cgagcttgtt gatcccgttg aagtcgctga agaagatccg ccccccgggt
     3661 ccgatctcga acgacagcgt gctccctccg tggttgtaca ccaccgagtg gcccgcgatc
     3721 gcgtcacgtt ggccgttgaa cacgatgcgc gtgacctccc cgttgttcac ggcgccg
//
LOCUS       DASUYD010000101        11724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_52980, whole genome shotgun sequence.
ACCESSION   DASUYD010000101 DASUYD010000000
VERSION     DASUYD010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52980"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VF195_05565"
     CDS             complement(<1..1199)
                     /locus_tag="VF195_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009182657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX6580323.1"
                     /translation="MRVETGDTAWVLVSAALVMFMTPGLALFYGGLVRAKNVLATVMQ
                     SLIALGVVTVLWVLVGYSLVFGPDHGLLTGGLEYVGLRGVGADPMALAPSVPASAFMV
                     FQMMFAVITPALITGAFAERMRFGGYLLFLGLWSLLVYVPVAHWVWGGGFLGVAGVGA
                     LDFAGGTVVHVNAGAAALATALYLGRRRGFGTGALRPHNVPMVLLGAGILWFGWFGFN
                     AGSALSAGGLASSAFVATHLGAAGGMLGWLVPERLRHERATTIGAATGAIAGLVAITP
                     ASGYVAPLPALAIGLAAGVVCFLAVELKGRLRLDDSLDVVGVHMVGGIVGALLTGVFA
                     SLAVNPAGADGSLAQVARQAAAVGVTLCFSFLATMAILRLVDRTLGLRVSEDVEEEGL
                     DRSIHGE"
     gene            complement(1380..2261)
                     /locus_tag="VF195_05570"
     CDS             complement(1380..2261)
                     /locus_tag="VF195_05570"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580324.1"
                     /translation="MSTIATGCTDSDAGQMRRASTGGDGVIVVGVSGAFAENQIVAEM
                     YAQVLEDAGYTVERRLDLRSREISQNALEAGAIDVKPEYLSSLLLFLDPNTEASADAA
                     AVARQTAQLLGSRGLTLLTPSPAEDTNQFVANAETAQRFDLTTVSSLASVADQLTFGG
                     PPECPQRPLCLPGLHEVYGVIFDDFTPLDAGGPLTVDALKSDEVQIGLLFSTDPSIEQ
                     NGFVPLEDDKRLQDAENITPVIRSEMLSDEVRRLLDLVSARLSSETMTELVGRVVIDG
                     QDVANVATGFLTSNNLL"
     gene            complement(2375..3718)
                     /locus_tag="VF195_05575"
     CDS             complement(2375..3718)
                     /locus_tag="VF195_05575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580325.1"
                     /translation="MDEIETSTTGRERSRWRLRLQQLRLRFRDPGLEKAYRADRFRHV
                     LGNIRFAFLAGIALWVTWGLLLRPHMLALSDQRLDLLMRFGVFIPILIVGLGLTYTRF
                     FGRVWEWVSVAIAGATLLLWVFYVSRISTLPAEYGYVGVILITAFSYTLLQLRFVSVA
                     LITVFGIAAYLPYALTARYISDVSLVLAALYLVSFGMLGGLAAYRLERFTRDLFLRER
                     QVEQERTRSDGLLLNMLPVAIVERLKTSSGGRIAQSFDRVSVFFADAVGSTEQAARSS
                     PEAFADALDALFRHFDEIAMRHGLEKIKTIGDAYLAVAGAPTPMADHADAALGVAAEI
                     LSEAGKIRWPSGDPIVVRGGIATGPAVAGVIGDRRFAYDLWGDTVNLASRLETSAAPG
                     RFLISESTIGELRDRYDFGPPEILEVKGKGPTTVRVLLGRHSNVPITVPLASEVE"
     gene            complement(3817..4962)
                     /locus_tag="VF195_05580"
     CDS             complement(3817..4962)
                     /locus_tag="VF195_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6580326.1"
                     /translation="MSSIPSLVPRDRYAALERCTYLNQASLGLIPVPTIDAMDTFLKE
                     TAQFGNLYLSDEQEAAILDGVRSAGAEVLGTSSDAVAVVAGASEGLGQVTSLLDPEHG
                     TVLLVGSDFPSVTYPWLAAAERRGISLRFIDDNADRDLTQDLVEAIDRTTVVVAFSVV
                     QYATGTRVDGPRVAERAHEVGARVVVDATQLAGAGIVDAVGWGADALVTSGYKWLSAH
                     GGVALLALAPDLVHRLPSLVGWKGTQHPFAFDPLALRLADDARRFELSTMSYVSAIGL
                     ESSIAMLSSLGFERIDQHARRLATHLVEGVAELGWRPFRPLGDPSAASHIVSLRHADH
                     DPGVTAERLASESRIICSGRAGGLRISLHAYNAGDDVDRLVESLRRL"
     gene            5038..5205
                     /locus_tag="VF195_05585"
     CDS             5038..5205
                     /locus_tag="VF195_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580327.1"
                     /translation="MASNVQLLDPAAAGTLFLSPARFAVFEENAGATNSSTARFESHG
                     IFIVQRPRRSA"
     gene            complement(5367..6041)
                     /locus_tag="VF195_05590"
     CDS             complement(5367..6041)
                     /locus_tag="VF195_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580328.1"
                     /translation="MQQHRLRTVGVAAIALFVLVACDADDEAPATAQPSANAVTNMNA
                     EEVATQFVEAYGAFDAEEAITYLAPDAAILELITSVGAHRGVSGTLDELRLVISLLRA
                     EGYRQTLDSCEGQGDSASGTMVNCTFDFHLFGSDRLGFGPYGGSSFDLTVLDGEIVRA
                     GAAFEVEEFSREMWEPFEAWVSEAYPDDATAMYDDENHTGVRLTEESVRLWRRHVRGY
                     VEVMGS"
     gene            complement(6075..7166)
                     /locus_tag="VF195_05595"
     CDS             complement(6075..7166)
                     /locus_tag="VF195_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580329.1"
                     /translation="MIDERELLEQAVRRFEPEPGLTERIYHRRDRKRRNQRIAAGVVG
                     IAVFVAAVWIVTTGGSLSRSETPPTPGGPGSSVGVRDMPEVDYLLDLNTGQERPLPNA
                     ITRLHDAGGEVGQFAASRDGSRLAFVKTSEEGSPQIFVASIDGTGVRQLTHHPFGVTS
                     PAWSPDGTTIAYAKSSPIGTGRLFVVGVTSGATRALGVRGSDPQFTPDGSSVFYSRGS
                     PAPTPMTVPAAGGEGTVLIDPGPGLLDAFSGSISPDGSQVTYLGSGSPLAPDGSPLMF
                     HGEEVTHAGPGRFVSRLDGTGFRLLPGGVSNPAGTWSPDGTRIVCSGEDGISGGGVIV
                     VDVATGNFTRVAEGRIAIWLDDHTLLVEV"
     gene            complement(7163..7693)
                     /locus_tag="VF195_05600"
     CDS             complement(7163..7693)
                     /locus_tag="VF195_05600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580330.1"
                     /translation="MDEPLPLLTGSEPPAVPLAETASFEGFVIENQTRLFGCLCLMTG
                     SRHEAEEIAQEAFVRVLERWDRVRGLDDPTGYLFVTAMNVSRQRLRRAGIALRRSIGV
                     APSEDVFGPVEDREVVLDALTRLPLDQRAALVVTALLGYSSEEAGRMLGARPSTIRAR
                     ATRARTALRDLIGVQR"
     gene            7942..8367
                     /gene="msrB"
                     /locus_tag="VF195_05605"
     CDS             7942..8367
                     /gene="msrB"
                     /locus_tag="VF195_05605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX6580331.1"
                     /translation="MDNSKQQRTTIPQTEQEWRTRLTPEQYEVLRNKGTEPPFTGAYA
                     STKDPGVYRCAGCGAALFSSTTKYESGTGWPSFWEPIEPGAVELHDDRTLWMHRTEVT
                     CARCGGHLGHVFSDGPQPTGDRYCMNSVSLELEPESHGE"
     gene            complement(8517..9125)
                     /locus_tag="VF195_05610"
                     /pseudo
     CDS             complement(8517..9125)
                     /locus_tag="VF195_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018505247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            9207..9800
                     /locus_tag="VF195_05615"
     CDS             9207..9800
                     /locus_tag="VF195_05615"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6580332.1"
                     /translation="MNRNPARGIRGCRLTTCLRVPSAASPTNWTASRGHRCKPTRSAS
                     CSHTTDGRPASCSASSTASTRPCGRGRTSWASGVLGGILVHHLGASQRWRIGFQTQGA
                     DEGPEPELEPLPTIDELRERWEAEWTAVDAWLPTLTDDFVAYAHEGVPVWQMLVHVVN
                     HGTQHRAEAAALLTGEGRSPGELDLIDYAEERAAGGN"
     gene            9866..11385
                     /locus_tag="VF195_05620"
                     /pseudo
     CDS             9866..11385
                     /locus_tag="VF195_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011891634.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
BASE COUNT         2058 a         4007 c         3839 g         1820 t
ORIGIN      
        1 ctctcgccgt ggatcgagcg atccagcccc tcctcctcga catcctcgct cacccgcagg
       61 cccagggtcc gatccaccaa ccgaaggatg gccatcgtcg cgaggaacga gaagcagagc
      121 gtgacaccaa cggccgccgc ctgccgcgcg acctgcgcga ggcttccatc cgctccggcc
      181 ggattcaccg caaggctcgc gaacaccccg gtgagcagcg cacccacgat gccgccgacc
      241 atgtgcacgc ccaccacgtc gagcgaatcg tcgagtcgga gccgtccttt gagttcgact
      301 gccaggaagc acaccacgcc ggcggccagg ccgatcgcca gagccggcaa cggcgcgacg
      361 tagccggagg cgggcgtgat cgccacgagg ccggcgatcg cacccgttgc cgcgccgatc
      421 gtggtggcgc gctcgtgtcg aaggcgctcg ggaacgagcc acccgagcat gccgccggcg
      481 gcaccgagat gggtcgcgac gaacgcgctc gatgcgagcc cgcctgccga cagggcgctt
      541 ccggcgttga agccgaacca accgaaccag aggatccccg cgccgaggag caccatcggc
      601 acgttgtgcg gacgcagcgc gcccgttccg aacccacgcc gcctgcccag atacagtgct
      661 gtcgcgagcg cagccgcgcc cgcgttcacg tgcacgacgg ttccgcccgc gaagtcgagc
      721 gctcccacgc cggcgacgcc gaggaacccg ccgccccaca cccagtgcgc gaccggcacg
      781 tacacgagca acgaccagag cccgaggaac agcagatagc cgccgaagcg catccgctcg
      841 gcgaacgccc cggtgatgag cgccggcgtg atcaccgcga acatcatctg gaagaccatg
      901 aacgccgagg ccggaacgct gggcgcgagt gccatcggat cggcgccgac accccgaaga
      961 cccacgtact ccagtccgcc ggtgagaagg ccgtgatcgg gcccgaagac gagcgagtac
     1021 cccacaagca cccagagcac cgtgaccacc ccgagtgcga tcagcgactg catcaccgtt
     1081 gcgagcacgt tcttggcacg tacgagcccg ccgtagaaca aggcgagacc cggcgtcatg
     1141 aacatcacga gtgccgcgga cacgagcacc cacgcggtgt caccggtctc gaccctcatc
     1201 gccgctcctc ctcgatggcg tctcgcgagc cattgagagg cctgtgaggc cgagtcggaa
     1261 cgagccgact gtaacgagtt cgttaatcca cgagcacaag gaacgtgtgt cgtcgaatcc
     1321 ccgagcagat gtcggcgtca cgatcgagca acggccggag ttcggagccg acaggccagt
     1381 tacagaaggt tgttcgaggt caggaatccc gtggctacgt tggcgacgtc ttgtccgtcg
     1441 atcacgacac gccccacgag ttcggtcatc gtctccgaag agagccgggc gctcacaaga
     1501 tcgagcaacc tccgaacctc gtcgctcaac atctccgaac ggatcacggg cgtgatgttc
     1561 tccgcgtcct ggaggcgctt gtcgtcttcg agcgggacga atccattctg ttcgatgctc
     1621 gggtccgtgg agaacagcaa cccgatctgc acctcgtcgc tcttcagcgc gtcgacggtc
     1681 agcggccctc ccgcgtccag cggcgtgaag tcgtcgaaga tgaccccata gacctcgtgc
     1741 aatcccggga ggcagagcgg ccgttggggg cattccggtg gtccgccgaa cgtcagctgg
     1801 tcggcgacgg aggcgaggga gctcaccgtg gtcaggtcga accgctgggc cgtctccgcg
     1861 ttcgccacga actgattggt gtcctcggcg ggtgatggtg tgagcaacgt gagccctctg
     1921 gacccgagca gctgggcggt ctgccgggca acagcggcgg catctgcgga cgcctccgtg
     1981 ttcgggtcga ggaacagcag caacgacgat aggtactccg gcttcacgtc gatggcgccg
     2041 gcctccaggg cattctgcga gatctctcga gaccgcaggt caagccgtcg ctcgacggtg
     2101 tagccggcat cctccaacac ctgcgcatac atctccgcga cgatctggtt ctcggcgaag
     2161 gcgccggaca cgccgacgac gatcacccca tcgcctccgg tggacgcacg ccgcatctgg
     2221 cctgcatcgg agtcggtgca tcctgtggcg atggtgctca ccaccacgac catcgccgcg
     2281 tacatcaggc tgcgatggaa gcccctgatc ggtctccgga gaggaagcgc ccggatccag
     2341 ccgcggatgg ttggggcgat ccttcttgtc gccgtcattc gacctcggag gcaagcggca
     2401 ccgtgatcgg gacgttcgag tggcggccca ggaggacgcg cacggtcgtg ggccccttgc
     2461 ccttcacctc gaggatctcg ggcggcccga agtcgtaccg atccctcagc tccccgatcg
     2521 tcgactcgga gatcaggaag cggccgggag ctgcgctcgt ctcgagcctg ctcgcgaggt
     2581 tcacggtgtc gccccacagg tcgtacgcga acctccgatc accgatcact ccggcgacgg
     2641 ccggccccgt ggcgatcccg ccccgcacca cgatcggatc gcccgacggc catctgatct
     2701 tcccggcctc gctgaggatc tcggccgcca ccccaagggc tgcgtcggcg tgatcggcca
     2761 tgggggtcgg cgcgccggcg acggccaagt acgcgtctcc gatcgtcttg atcttctcga
     2821 ggccgtgccg catggcgatc tcgtcgaaat ggcggaacag cgcatccagg gcatcggcga
     2881 acgcctccgg ggacgaccgc gcggcctgtt ccgtggatcc caccgcgtcc gcgaagaaca
     2941 cgctcacgcg atcgaagctc tgcgcgatcc gtccgccgga cgaggtcttg agccgctcga
     3001 cgatggccac gggaagcatg ttgagcagga gcccgtcgga gcgcgtccgt tcctgctcga
     3061 cctgacgttc gcggaggaac aggtctcgcg tgaaccgctc cagccgatag gcagccagcc
     3121 cgcccagcat cccgaacgag actaggtaga gagcggccag caccagactc acgtcggaga
     3181 tgtaccgtgc cgtgagggca tacggaaggt acgcggcgat accgaacacc gtgatcagcg
     3241 cgaccgagac gaaccgtagc tgcagcagcg tgtagctgaa cgccgtgatc aagatgacgc
     3301 cgacgtatcc gtactccgca ggcaacgtcg atatgcgcga gacgtagaag acccagagca
     3361 ggagggtcgc ccctgcgatg gcgaccgaca cccattccca gacccgtccg aagaaacgcg
     3421 tgtacgtgag ccctaggccg acgatcagga tggggatgaa cacgccgaac cgcatgagca
     3481 ggtcgagtcg ctggtcggac agggccagca tgtgggggcg taggagcaag ccccacgtga
     3541 cccagagggc gatcccagcc aggaaggcga agcggatgtt gccgagaacg tgacggaaac
     3601 ggtcggcgcg atacgctttc tcgagccccg gatcgcggaa ccgcagccgc agctgctgaa
     3661 ggcgaagccg ccacctggat cgctcacgac ccgtcgttga tgtctcgatc tcgtccatct
     3721 gcctcttcta cctcccggct gcaatgatgg ccgctcggca cccccttgtc agccgaagcc
     3781 cgggggttcg gatcagcagc gcgaccagcg gagggcttag agccgcctca gggactccac
     3841 gagccgatcg acgtcgtcgc cggcgttgta ggcgtggagg gagatcctga gcccgcccgc
     3901 ccgaccgctg cagatgatcc gggactcgga agcgagacgt tccgcggtca cccccggatc
     3961 gtgatcggcg tgccgcaggg acacgatgtg agacgccgcc gatggatcgc ccagcggccg
     4021 gaacgggcgc catcccagct cggcgacccc ctcgacgagg tgcgtcgcca agcgccgcgc
     4081 gtgctggtcg atgcgctcga accccagcga cgagagcatc gcgatcgatg actcgagccc
     4141 gatggcggac acgtacgaca tcgtcgagag ctcgaagcgg cgcgcgtcat ctgcaaggcg
     4201 aagcgccagc ggatcgaagg cgaatggatg ctgggttcct ttccatccga cgagggaggg
     4261 aaggcgatgt accagatctg gagcgagcgc cagcagcgcc acgccgccgt gggccgacag
     4321 ccacttgtag ccgctggtca ccagcgcgtc cgcgccccat ccgacggcgt cgacgatccc
     4381 cgcaccggcg agttgcgtcg cgtctaccac gacgcgggcg cccacctcgt gcgcccgctc
     4441 cgccacccgc gggccgtcga cccgcgtgcc cgtcgcgtac tgcaccacgc tgaaggcgac
     4501 gaccaccgtg gtccgatcga tcgcttcgac gagatcctgc gtgaggtccc ggtcggcgtt
     4561 gtcatcgatg aaccgcagcg agatcccacg acgctcggcg gcggcgagcc acgggtacgt
     4621 cacgctcggg aagtccgatc cgacgaggag gacggtcccg tgctcggggt cgagcagcga
     4681 ggtcacctgg cccagtccct cgctcgcgcc tgcgacgacg gccaccgcat cggatgaggt
     4741 tccgagcacc tcggcgcctg ccgagcggac gccgtccagg atcgcagcct cctgttcgtc
     4801 gctgagatag aggttcccga actgcgccgt ctccttcagg aaggtgtcca tcgcatcgat
     4861 cgtcggtacg gggatcaacc ccagcgacgc ttggttgagg tacgtgcagc gctcgagggc
     4921 ggcgtagcga tcgcgcggaa cgagcgaggg gatcgaggac atctgcgcct ccaaggggaa
     4981 ccggccccgt cggatgatgg atcatcgccg caacgccttg ccgacgttcg ggagccgatg
     5041 gcgtcgaacg tccagctgct cgatccagcc gctgcaggca cgctgttctt gtcgcctgcg
     5101 cggttcgcgg tgttcgagga gaacgccgga gcgacgaata gcagcacggc aagattcgag
     5161 tcgcacggca tcttcatcgt gcagcgcccg cggcgatctg cttgatcaca ggtgggtcat
     5221 gccgtcccca tgtgcgcggc gggatggact gctcgggctc gcggccacgg cctgtgggcg
     5281 tcctggcatc gcgcgtcgaa ccccgttccc acttagtggg tagatgttga atctcccatc
     5341 gaggcgcaga cgtcgcgcgt cgccggtcac gaccccatca cctcgacgta gccacgaacg
     5401 tgccgcctcc agagtcggac cgactcctcg gtgagtcgca ctcccgtgtg gttctcgtcg
     5461 tcgtacatcg ccgtcgcgtc gtccgggtag gcctctgaca cccacgcttc gaagggctcc
     5521 cacatctccc gggagaactc ctcgacctcg aaggcggcgc ccgctcggac gatctccccg
     5581 tcgagaacgg tgagatcgaa agagctgccg ccgtacggac caaacccgag ccgatcggat
     5641 ccgaacaggt ggaagtcgaa cgtgcaattg accatggtgc ctgacgccga atcgccttgt
     5701 ccctcacaag aatcgagcgt ctggcgatac ccctcggctc tgagcaggga gatgaccagt
     5761 cgcaattcat ccagggttcc cgagacaccc cggtgggccc ccaccgatgt gatcagctcg
     5821 aggatggcgg cgtcgggtgc caggtacgtg atcgcctcct cggcgtcgaa ggcgccgtac
     5881 gcctcgacga actgtgtggc cacctcctcc gcgttcatgt tcgtgacagc gttcgctgat
     5941 ggctgggccg tggccggagc ctcgtcgtcc gcgtcacagg ctacgagaac gaagagcgct
     6001 atcgccgcca caccgaccgt tcgaagccga tgctgttgca tggtgcaccc ctccatgtcc
     6061 cgttcagcac gtcgtcagac ctcgacgagc agcgtgtgat cgtcgagcca gatcgcaatg
     6121 cgtccctccg caacgcgagt gaagttgccg gtcgcgacgt ccacgacgat gaccccaccc
     6181 cccgagatgc cgtcctcgcc ggaacacacg atccgggtcc catcggggga ccacgttccc
     6241 gctggattcg agacgccccc gggaagaagc cggaatcccg tgccgtcgag cctggacacg
     6301 aaccgccccg ggcctgcgtg ggtaacttcc tcgccgtgga acatcagggg cgatccgtcg
     6361 ggggcgaggg gcgagccgct cccgaggtag gtcacttgcg agccatcggg tgagatcgag
     6421 ccgctgaagg cgtcgaggag gccggggccg gggtcgatga ggaccgtgcc ctcgcctccg
     6481 gcagctggaa ccgtcatcgg cgtgggcgcc gggcttcccc tcgagtaaaa gacagatgag
     6541 ccgtccggag tgaactgcgg gtcgcttccc ctcaccccga gcgccctggt cgcaccgctc
     6601 gttacaccga cgacgaagag gcggccggtg ccgataggac tgctcttggc gtaggcgatc
     6661 gtggtgccat ccggcgacca tgcgggcgac gtcacaccga aggggtgatg cgtcagctga
     6721 cgaacccccg tcccgtcgat actggcgacg aagatctggg ggctcccttc ttcgctggtc
     6781 ttgacgaatg cgagtctcga gccgtcgcgc gaagccgcaa actgcccgac ctcaccaccc
     6841 gcgtcgtgca agcgggtgat agcgttcggc aacggcctct cttgtccggt gttcaaatcg
     6901 agcaagtaat cgacctcggg catatcgcga actcccaccg aggatcctgg accgccaggc
     6961 gtgggcggcg tctccgagcg ggaaagcgag ccgccagtcg tcacgatcca gaccgccgcc
     7021 acgaacaccg cgatcccgac cacgcccgcc gcgatgcgct ggttccgacg cttgcggtcg
     7081 cgccggtgat agatgcgctc ggtaaggccc ggctccggct cgaagcggcg caccgcttgt
     7141 tccaggagtt ctcgctcgtc gatcatcgtt gcactccgat caggtctcga agggcggtgc
     7201 gtgccctcgt ggctcgagcg cggatcgtcg agggacgagc gccgagcatc cgtccggctt
     7261 cctccgagga gtagccaagg agcgccgtga ccacgagcgc cgcccgttga tccaggggta
     7321 gccgcgtcag agcgtcgagg acgacctctc gatcctccac agggccgaac acatcctcgc
     7381 tcggcgctac gccgatcgac cgccggaggg cgatgcccgc tcggcgcaga cgctgtcgcg
     7441 agacgttcat cgccgtgacg aagagatagc cggtcggatc gtcgagaccc ctcactcggt
     7501 cccagcgttc gagtacgcgg acgaaggcct cctgcgcgat ctcctcggcc tcgtggcggc
     7561 tccccgtcat gaggcagagg cacccgaaca gccgagtctg gttctcgatc acgaagccct
     7621 cgaagctcgc cgtctcggca agcggtacgg ccgggggctc cgacccagtc aggaggggga
     7681 gcggttcgtc catcacacac aagaatcctt cgtgggggat tctgctacga ggcgcggcga
     7741 gtttgcatcg gtcgccgtgc ggcgcgagct cgggggatac aggcaaccag gcagatcgac
     7801 gttagctcgc ggggccgatc ggcgtccctg ctcagagccg tcctgatatc gggcagtgga
     7861 gcttggagcg ccgctcgcga cggtgggaag cgcgtaacaa aacgacggct gatgagacag
     7921 agtctgcgag gaggagatga gatggacaac tcgaagcaac agcgcacgac tatcccccag
     7981 accgagcagg agtggcgcac ccgcctgacc cctgaacagt acgaggtgct gcgtaacaag
     8041 ggaacggagc cgccgttcac gggcgcgtat gcgtcgacga aggaccccgg cgtctacaga
     8101 tgcgcgggat gcggggccgc gctattcagc tcaacgacga agtacgagtc ggggaccggt
     8161 tggcccagtt tctgggagcc gatcgagccc ggggccgtcg agctgcacga cgaccggacc
     8221 ctctggatgc atcgcacgga ggtcacctgt gcgcgatgcg gtggccatct agggcatgtg
     8281 ttctccgacg gaccgcagcc gacgggggat cgctactgca tgaactcggt ctcgctggaa
     8341 ctcgagccgg agtcgcacgg ggagtaggcg accgacctga acctcccggc tctggatttc
     8401 agtcactctg tggcgcaccg cccttggacg atagccgctg aagccatcct tgctcaggtc
     8461 aacgcataag gagcgttgtc ggcggatcga gagggtgggt agcgcaggca cgagtcgtcg
     8521 ttgtggtagt cgacgtaggt gtcgtccttc ccgaggtgga tgttcgcgag tgcgcttccc
     8581 cagaagaccc ttcctgaggc tgggatgtgg tagcgctcgt agagctcgag tgacttctct
     8641 tcgctgaagg tgttcgcgaa cacgtagtgc cactgctttg gcgtgaagcc gaccgctcga
     8701 tgccggttcg ccgggttctt caggatgggg aacccggact tgatctcgga gagcggaacg
     8761 accttcacgc cctcggtcgg cgctgagtcg atcgccaccc caaccgcacc gaagccgtgg
     8821 tcgagcaaga tctgggtgaa cacgccgccg gccgagtgcc cgatgatgat cggcggacga
     8881 tccaattccc cgatcatgtt ctcgagcatg tcgatgatct ccggtacctg gagctgctcg
     8941 atcggcgtgg ggtcagcgcg gagcgcctcg acctcgacct cgaaccccgg gtatgcaggt
     9001 gtgaggaccg tatagccctt cgcctcgtaa tacttcgtcc agtgctccca actcctcggc
     9061 gtcacccaga agccgtggat caggacgatc gtgtcgggct tggtcttgac cggggcctct
     9121 gccatcgcgg ctccttccct cgtcgacccg tccaccctgg gacgcccgag acgggtggga
     9181 caaggtcagg tggcccgacg gagacgatga accggaatcc ggcgcggggg atccgtggat
     9241 gtcgcctaac cacatgcctt agggtgccgt ccgcggcatc acccaccaac tggaccgcat
     9301 cgagggggca ccgatgcaag ccgacgagat ccgcttcctg ttcgcatacg accgatgggc
     9361 gacccgccag ctgctcggcg tcctcgacgg cctcaacgcg gccctgtggg cgcggacgga
     9421 cgtcgtgggc gagcggggtc ctgggaggca tcctcgtcca tcacctcggc gcttcccagc
     9481 gctggcgcat cggtttccaa acacagggcg ccgacgaagg acccgagccg gaactcgagc
     9541 cgttgccgac gatcgacgag ctgcgcgaac ggtgggaagc ggagtggacc gctgtcgatg
     9601 cgtggttgcc aaccctgacg gacgacttcg tcgcgtacgc ccacgaagga gtgcccgtct
     9661 ggcagatgct cgtgcatgtc gtgaatcacg ggacgcagca ccgagccgag gcggcggcgc
     9721 ttctcaccgg cgagggccga tcgccgggcg agctcgacct gatcgactac gccgaagaac
     9781 gagcggccgg cggcaactag gagactgctg aacaaatcgc ctcgagatgc tcgcgatcgg
     9841 gtgcgcgagg atcgaggctt tgggcatgca aggacacagc gatcccaatc gtgagctgct
     9901 tgatgcggcg gccctctgcc gcaagctcgt cccggagggc agcgtcgagg ggttcctcgc
     9961 cgatcaccgc catgagctgt tccccgatga gctgttcgcc gacctgttcc cctcgggtcg
    10021 ggggcgaccg tcggttcctt ccgacgtgat cgccaccgtg atggtgctcc aggccctcga
    10081 gggcctgtcc gatcgcgacg ccgctcgggc gcttcgcgac cggatcagct ggaaggtggc
    10141 gtcgggtctg gccctggacg acaccgggtt ccaccactcc gtcctgacct actggcgcac
    10201 ccggctccgg gcctcggagc gtccgcagcg gatcttcgac gcggtccggg ccgtggtcga
    10261 tgccaccggt gtgctggggg caaggcgcgc cgggccttgg actccacgct gcttgacgac
    10321 gcggtggcca cccaggacac cgtcacccag ctcatctcgg tcatccgccg ggtccgccgg
    10381 gaggtcccca gcgccgctac ggtggacctg gtcgctcacg actacgacgc cggcgccaag
    10441 ccgctcatcg cctgggacga cgcgctggcc aaggagcagc tcgtgagcgc cctggtaaac
    10501 gatgccctct gcctgatcga agcgaccgag tgcaccgagc tcaccgcctc ccagcaagac
    10561 gccgtcgggc tgctcgccct ggtcgccggc caggacgtgg agcccgcaga cgtggagggg
    10621 tcgtggcgga tcgcccgcgc cgtggcgcct gaccgggtga tctccacggt ggacccggag
    10681 acccgccaca tgcacaagtc ccgggcctcc taccgcgacg gctacaaggc gaacatctgc
    10741 gtggagcccg agaccgggct catcaccgcc tgttccctca cccccgccaa cgccgccgac
    10801 gggccggcag gcgtggccct gatcgcctca gaggacgccg ggctggaggt gctggccgac
    10861 tcggcctacg gctcgggtga ggtccgggcc gccctggcca aggccgaaca cctggcgacg
    10921 atcaagccga tcccgcttcg cccggccatc cccgccgggt tcacgctcga tgagttcacc
    10981 atcgataccg tcaaaggtgt cgtcacctgc ccccagggca tcaccgtgtc gatcacccca
    11041 cgcggcaacg ccgtgttcgg cgcacggtgc cgccgatgcc cgcttcggga ccgatgcacc
    11101 agctcgagga agggtcgcac gatcacggtc cacccacacc atgacctgat gctcgccgcc
    11161 aggaagcagg cccgaaccgg gcggttccag aactcctacc gacgacatcg accgatggtg
    11221 gaacgctcga tcgcgtggct ggtcgcacac ggccaccgtc gggtccgcta ccgcggcatc
    11281 gcgcgcaacc aactggggct atctcaccgg atcgccgcca tcaacctgcg gaggctcgtg
    11341 aacctcggcc tggaccacga cggaggttgg gtgctcacga cctgaccgac cgtgggcggg
    11401 agcctcactc ggccgacgat ggcgtgacca accctcgatc agccaccaat ctgtctcggc
    11461 gacccgctga cgttctccga tcagcacgcg ggcccgtctg cctcccagat atcgccttct
    11521 tcagcagtct cctagggctc ctcgaccgtc tactagcgac gcccgccgcc ttcgacgcca
    11581 gccatattct gggtcaggac tctttcctgc tcgcagggca ggtttgccag ctcagcgatg
    11641 aatgtcggtt ccggtgggga ggattgtcga catgacgaca acgtctcgcg aactctacgc
    11701 gcccacctca gagggagagg ggcg
//
LOCUS       DASUYD010000102         4537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_714446, whole genome shotgun sequence.
ACCESSION   DASUYD010000102 DASUYD010000000
VERSION     DASUYD010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4537
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714446"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(283..597)
                     /locus_tag="VF195_05625"
     CDS             complement(283..597)
                     /locus_tag="VF195_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580333.1"
                     /translation="MSAKTETLAAPARPIASFVLAIVVTLAVGATAGSLITQAVTPEV
                     APVDVGIVGIAPWDQQKLDAMEGRQQAVTVETDGPGIAPWDQDKLDAAGSLQASAQHI
                     SS"
     gene            complement(833..3628)
                     /locus_tag="VF195_05630"
     CDS             complement(833..3628)
                     /locus_tag="VF195_05630"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580334.1"
                     /translation="MTGDVIGRESELATLHSFLDSIPTGPSALLLSGDPGIGKTTVWK
                     EGLAGAIQRGYLTLSCGPVEAETRLSYAALGDLLEPVLEEALPTVPEPQRQALEVALL
                     RSPRSGARADQRAVCLAVLGCVRSVASTSPVVVAVDDIQWMDLPSVRVLQFVVRRLKD
                     EPVGLMTVARGARAEYDPLGVVPAFADDRVLAVHVGPLSQDALERVLLAKVGEGFSRT
                     TLLSLYEMSGGNPFFAREIGLALLRRGGDVTAGERLPIPDRLQDLIEDRLGGLPARTV
                     EALEVVSATSAPTFEAIAAATDPSEVDRRLGPAIEHHVVEVVGDRLRFTHPLLASAVY
                     QKIPLARRRELHARLATIVLDPEERARHLALSVEGPDVVVADALEEAALLASSRGAPQ
                     SAAELWEMARRATPRDRGEDLVRRTHQAGVAHFESGDTSLARSVLEQAVDLSSAGPAR
                     ARVLLDLGMGIAEAEGWRGAWAVFEAARGEAGDDLALRALIEQNLGYSWLFRGDLAAS
                     ERHARAALELAEELQDPRVMAEAFQAYPFVEFLLGRGVDQELLDRGIALEGHLEGEFK
                     SHVLRASFVVAQLLKFTDRLDEARRTFTELLGDAVSHGVTGPIPQILYHLAELECRAG
                     NWDTALEHARESRAVAQRISMGALSSEGHFVVGLVEASLGRADPARLAALEGLQVAEA
                     AGEILLVIPNLAVLGFLELSLGRPAEADAYLSRAVELEQAMGVREPAYFRIVPDEVES
                     LVALGQLDEAEALLAPFEDAGRILDRAWAMATGARCRALVFAARGDLQAASDAADEAV
                     RHHDRLSLPFELGRTLLVRGAVGRRAKRKREARDTLTEALEVFDGLGAALWADKARAE
                     LARIGGRAPSSLDLTPSEDRVAALVAAGSTNREVADALFVSIHTVEANLKRIYRKLGV
                     RSRTELASKFPSGSSGS"
     gene            3996..4328
                     /locus_tag="VF195_05635"
     CDS             3996..4328
                     /locus_tag="VF195_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEX6580335.1"
                     /translation="MGDLTILVLRFVLAAVLAGSLFVQVVMVPLLAADLDGLDPDYAH
                     LRTPLLVIAVLGIAVLGIVTIQVVLVCVWRLVTMVRRGTVFPHAAFRYVDVVAEAHHL
                     QAELDEVI"
     gene            4328..>4537
                     /locus_tag="VF195_05640"
     CDS             4328..>4537
                     /locus_tag="VF195_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015792567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6580336.1"
                     /translation="MPIVVDIDVMLAKRKMSVGALADRVGITPANLAVLKNGRAKAVR
                     FTTLAALCEALGCQPGDLLRWDPDAR"
BASE COUNT          723 a         1583 c         1531 g          700 t
ORIGIN      
        1 cactcaccga gcccgccatg acaagctcaa ggagttcggg gcgctcgcca atcctggagg
       61 caggccgagg aaggtggccc gagatgtcgc ataggtgccg gagccattcc gggacacggg
      121 tggtgagact tcacagttgg cccaggtcag atgaccagga tgcgatcgtc tcgagatccg
      181 tatggcaggt cggctgctcc atgccgacct cctagtgccc gaggtgccat ggtcgatgcg
      241 ggtcctggcc ggtggtgcga ccgaccgacc cgcctcgacg tcctagctgc tgatgtgctg
      301 cgccgatgcc tggaggctcc ccgcggcatc gagcttgtcc tggtcccagg gtgcgatccc
      361 gggcccgtcg gtctcgacgg ttacggcctg ctgacggcct tccatggcgt caagcttctg
      421 ctggtcccac ggggcgatcc ccacgatccc cacgtcgacg ggcgcgacct caggcgtgac
      481 cgcctgcgtg atcaggctgc cggcggtcgc gccgaccgcg agcgtgacga cgatcgcaag
      541 gacgaacgaa gcgatcggtc gagcgggtgc tgcgagggtc tctgtcttgg cgctcatcgc
      601 gtgcctcctc gtgtcgctcg ggttggaacg gtgttgggtc ggcgcctccg gtgtttccgt
      661 ctcgacgcca tcaagacagg agatcccgtt ccgctgccat cgttcccgct ctcgtactca
      721 cgatccctcc cttcacgcgg gtaccgatat gggtcccgag gctagagaga gcatcgaccc
      781 gaggaatccc cgaaatccgt atcgatcgag acggtgaccg gtacgtcttg gctcaggaac
      841 ctgaggagcc agatgggaac ttcgaggcca gttccgtgcg cgagcgaacg cccagcttgc
      901 ggtagatccg cttgaggttg gcctccaccg tgtggatgct gacgaacagc gcatcggcca
      961 cctcccggtt ggtgctgccc gcggccacca gcgccgccac cctgtcctcg gacggggtca
     1021 ggtcgagcga cgagggagcc cgtccgccga tccgcgccag ctccgcacgt gccttgtccg
     1081 cccacaacgc ggcacccagc ccgtcgaaga cctccaaggc ctccgtcagc gtgtcccgag
     1141 cctcccgctt cctcttcgca cggcgcccca ccgctccgcg caccaggagg gtccgcccca
     1201 gctcgaaggg aagggatagg cgatcgtggt gacggacggc ctcatcggct gcatcagatg
     1261 cagcttggag gtcaccccgg gccgcgaaca cgagcgcccg gcatctggct cccgtcgcca
     1321 tggcccacgc cctatcgagg atccggccgg cgtcctcgaa gggcgcgagg agcgcctcgg
     1381 cctcgtcgag ctgccccagg gccacgagcg actcgacctc atccgggacg atccggaagt
     1441 acgcgggctc ccgaacgccc atcgcttgct cgagctcgac ggccctggac agatacgcat
     1501 cggcctcggc gggtcggccc agggaaagct cgaggaaccc taggaccgcc aggttcggga
     1561 tcacgaggag gatctcgccg gccgcctcgg cgacctgcag cccctcgagg gccgccagcc
     1621 tcgccgggtc ggcccgcccc agactggctt ccaccaggcc caccacgaag tggccttccg
     1681 acgacagggc ccccatgctg atccgctggg cgaccgccct gctctcccgg gcgtgctcca
     1741 gggctgtatc ccagttgcca gcccggcact cgagctcggc gaggtggtac aggatctgcg
     1801 ggatcgggcc cgtgacgccg tgcgacacgg catcacccag gagctcggtg aacgtccggc
     1861 gggcctcgtc caggcgatcg gtgaacttga ggagctgcgc cacgacgaag ctcgcccgca
     1921 ggacgtggga tttgaactcg ccctccaagt gcccttccag ggcgatcccg cgatcgagca
     1981 gctcctgatc gaccccgcgt ccgaggagga actccacgaa cggatacgcc tggaacgctt
     2041 cggccatcac ccgtgggtcc tgcagctcct cggctagctc cagcgcggcg cgagcgtgcc
     2101 gttccgatgc cgcgaggtct cccctgaaca gccaggagta cccgaggttc tgctcgatca
     2161 gcgccctcag cgcgagatcg tcccccgcct cgccccgagc ggcctcgaac acggcccagg
     2221 ccccccgcca cccttcggcc tcggcgatac ccatccccag gtccagcagc acgcgggccc
     2281 gagccggccc ggccgacgag aggtccaccg cttgctccag gacgctccga gccagcgagg
     2341 tgtccccgga ctcgaagtga gccacgcccg cctggtgggt ccgacgcacc aggtcctcgc
     2401 ctcggtcccg cggggtggct cggcgggcca tctcccagag ctcggccgcg gactgcggcg
     2461 ctccccggga ggaggccagc agggcggcct cctcgagagc gtcggcgacg acgacgtccg
     2521 ggccttcgac ggacagcgcc aggtggcggg ctcgttcctc cggatcgagg acgatcgtcg
     2581 cgagccgcgc gtgcagctcg cgacgccgag ccagcgggat cttctgatag acggcggacg
     2641 caaggagggg gtgggtgaac cgaagccggt cgccgacgac ctcgacgacg tggtgctcga
     2701 tcgcgggacc caggcgacgg tcgacctcgg aaggatcggt cgcggcagcg atcgcctcga
     2761 aagtcggagc cgacgtggcc gagacgacct ccagggcttc gacggttcgc gcagggagcc
     2821 ccccgaggcg atcctcgatg aggtcttgaa ggcggtcggg gatcgggagc cgttcgcccg
     2881 ccgtcacgtc acctccacgg cgaaggagcg ccaatccgat ctcccgagcg aagaaggggt
     2941 tgcctcccga catctcgtaa aggctcagga gcgtcgtccg ggagaaccct tcccccacct
     3001 tggccaggag cacccgctcg agcgcgtcct gcgacagcgg tcccacgtgg acggccagca
     3061 cgcgatcgtc ggcgaaggct gggacgacgc ccagggggtc gtattcggca cgggcgcctc
     3121 gagcgaccgt catcaagccg accggctcgt ccttcagacg ccgcacgacg aactggagca
     3181 cccggaccga agggaggtcc atccactgga tgtcatcaac ggccaccacg accggggagg
     3241 tggatgccac cgatcggacg catccaagca ccgccaggca aacggcccgc tggtcggctc
     3301 gggcgcccga tctcggggag cgcaagaggg ccacctcgag ggcctggcgc tgtggctccg
     3361 gcacggtggg gagtgcctcc tcgaggacgg gctcgaggag gtcaccgagg gcagcgtagg
     3421 acaggcgtgt ctcggcctcg acggggccgc acgagagcgt caggtacccc cgttgaatgg
     3481 cacccgccag tccctccttc cacacggtgg tcttcccgat gccgggatca cccgatagaa
     3541 gcagggcgct cggcccggtc gggatcgagt cgaggaagct atggagggtc gcgagctctg
     3601 actcgcgccc gatgacgtca cccgtcaaag gcgctcccgc gtcgatggac gccaggattc
     3661 taaggatggc gggtccgaac cttgcagacc cgtcgaggcc caggccaagt accggccgtg
     3721 taccaatcgc gtgccgggtc ggacctctct gggcgacttg catctctcga ccccccgagt
     3781 cgtgcctatg acctgccgaa gaggcaaaac cgctggccga agaggcaaga ccgctgtccg
     3841 gagagtcgtt caagttgctg ggcgcccaga cacacccccg cggcgagagc ttccacatgc
     3901 agtgttgacg ggacgactcg gtcgctgatg tccggtcggg atcaagctat cgattatcga
     3961 tatatcctca tcgtagatcg atcgaaggag ctctgatggg agacctgacg atcctcgttc
     4021 ttcgcttcgt gctcgcggcg gtgctcgccg gctcgctgtt cgtgcaggtg gtgatggtgc
     4081 ccctgttggc tgccgacctg gacgggttgg accccgacta cgcccacctt cgcacgccgc
     4141 tcctggtcat cgcggtcctg ggcatcgcgg tcctgggcat cgtgaccatc caggtcgtcc
     4201 tggtctgcgt gtggcgcttg gtgacgatgg tgcgacgggg gacggtgttc ccgcatgctg
     4261 ccttccggta cgtggacgtc gtggcggagg cgcaccacct gcaggccgag ctggacgagg
     4321 tgatctgatg ccgatcgtgg tcgacatcga cgtcatgctg gccaagcgca agatgtccgt
     4381 gggcgcactc gcggaccgcg tcgggatcac gcccgccaac ctcgcggtgc tcaagaacgg
     4441 gcgcgccaag gctgtgcggt tcacgacgct cgccgcactg tgcgaagcgc tggggtgcca
     4501 gcccggggac ctgctgcgct gggatcccga cgcccgg
//
LOCUS       DASUYD010000103         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_495858, whole genome shotgun sequence.
ACCESSION   DASUYD010000103 DASUYD010000000
VERSION     DASUYD010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495858"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..424
                     /locus_tag="VF195_05645"
     CDS             95..424
                     /locus_tag="VF195_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580337.1"
                     /translation="MDFEETLLKFGFHPATGRTPKGVQVLAAEPNPFLTYTVQAFDDG
                     SALFTWEFAVGEYLRTKGIQFGSDETLNQFMFPREDTRGPQDGAWLAEAVERARAQLA
                     SIRFDHP"
     gene            complement(519..956)
                     /locus_tag="VF195_05650"
     CDS             complement(519..956)
                     /locus_tag="VF195_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580338.1"
                     /translation="MSARVESPPIGGGTLGGSLVIAVVVALSIGALVGSLITHAIDSD
                     GGATRATTFVARGTPALTAVDVGNGSKAIEGPKLAERFTGLASANVWDAGKVQAMEGR
                     QLAEQFRSEAGSAVVWDAGKLEAMEGRQLAEEVRHRPHGGPLG"
     gene            complement(1161..2117)
                     /locus_tag="VF195_05655"
     CDS             complement(1161..2117)
                     /locus_tag="VF195_05655"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580339.1"
                     /translation="MLLLADGYEVQPPTFDDVEVVTEMLVATDLADTGVSETDADLIR
                     DQWSSPGVDLSEDAWVVTDPNGAVVAYANVSPDGEGKVKSWGVVHPEHRGRGLGAALL
                     DRIEARASERLRGIPSAVLHTAVNDAHAAAAALVRSRGFEHVRTFRHLQIDLDGSPQD
                     PGEPPAGIEIRGIEPERDLRRIHAIFVEAFSGEWGYRPIPFEEWIGMEVETPSYDPSL
                     WLLATDGDEAVGAQSGVVWGDRGWVGELGVRTPWRGRGIASAMLRRAFATFASRGLAR
                     VMLNVDSENSTGAVRLYEGVGMRTLRAWDVYEKPIRGDRRVS"
     gene            2168..>2534
                     /locus_tag="VF195_05660"
     CDS             2168..>2534
                     /locus_tag="VF195_05660"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX6580340.1"
                     /translation="MTTSVRPPGPDLSRLAALRAFRKDPLGLLERLAAYGDVVRIDVP
                     GSAAFLLNHPDLVHEVLVAEHRSFHKGPTIQAAKLLLGESLLTSEDDVHRRQRRLIQP
                     MFHHERIASYAASMVRFAER"
BASE COUNT          382 a          904 c          845 g          403 t
ORIGIN      
        1 gccggaccgc ggcagccttc gaccccaaac cggagctcga cctcgtggac ggggtgctga
       61 aggcggttcc gtaaacgcgg gataggctcc gtccatggac ttcgaggaaa ccctcctgaa
      121 gttcgggttc catcccgcca cgggccgaac gccgaagggg gtgcaggtgc tcgccgccga
      181 gccgaaccca tttctgacgt acaccgtgca ggcgttcgac gacgggtcgg ccctgttcac
      241 gtgggagttc gcggtcggcg aatacctgcg caccaagggg atccagttcg gttcggacga
      301 gacgctgaat cagttcatgt tcccgcgcga ggacacgcgt ggtccgcagg acggcgcgtg
      361 gctcgccgaa gccgtcgagc gggcgcgggc gcagctcgca tcgatccgat tcgaccatcc
      421 ttagccacct cggacggcgg cggaacgtcg gtcagacgat cgaaggccgg gctctcagat
      481 tcagcccagc cttcgcgtgc tccgccgttc gatcaggcct aaccgagggg gcctccgtgt
      541 ggtcggtgac gaacctcctc ggccagctgg cggccctcca tcgcctcgag cttgccggcg
      601 tcccagacga ccgccgagcc agcctcgctc cggaactgct cggccagctg gcggccctcc
      661 atcgcctgga ccttgccggc gtcccagacg ttggccgatg ccagcccggt gaaccgctcg
      721 gcgagcttcg ggccctcgat cgccttcgag ccattgccca cgtccacagc cgtcaacgca
      781 ggtgtccctc gcgcaacgaa ggtggttgca cgtgtcgccc caccatcgct gtcgatggcg
      841 tgggtgatga ggcttccgac caacgccccg atcgacaacg cgacgacgac cgcgatcacg
      901 agcgatccgc cgagcgtccc tccgccgatg ggcggtgact cgacccttgc gctcatcgcg
      961 cacctccctt cgatccttcc tgcgaacctt cgacgagcct gctccgatgg gatctcggtc
     1021 gcggtggcgg cgcgcacggt ctcggcgtga ccctcgtcac gggagcgcga ggcgatcgat
     1081 ctggatcgcg aagcggacag gaagcggcct atcgggctgg tcgtcatcga cctgcgatca
     1141 cggtggggcc gatgcttctt tcaggagacc cgacgatcgc cacgtattgg tttctcgtac
     1201 acgtcccacg cgcggagggt gcgcatcccc acgccttcgt agaggcgcac ggccccggtc
     1261 gaattctcgg agtccacgtt cagcatcacc cgggccagcc cgcgggacgc gaacgtggcg
     1321 aaggcgcgtc gcagcatggc cgaggcgatc ccgcgtccgc gccagggcgt ccgcacgccg
     1381 agctcaccga cccagccccg gtcgccccac acgacccccg actgcgcacc gaccgcctcg
     1441 tccccgtcgg tcgcgagcag ccacaggctc gggtcgtagc tgggcgtctc gacctccatc
     1501 ccgatccact cctcgaaggg gatcgggcgg tagccccatt ccccgctgaa tgcctccacg
     1561 aagatcgcgt ggatccggcg gaggtcgcgt tcgggttcga tcccgcggat ctcgataccg
     1621 gccggtggct cgccgggatc ctgcggtgat ccgtcgaggt cgatctgcag atgccggaac
     1681 gtccgcacgt gctcgaaccc gcgggatcgc accaacgccg cggcggcagc gtgtgcgtcg
     1741 ttgaccgcgg tgtggagcac cgctgagggg atacctcgca ggcgctcgga ggcgcgagcc
     1801 tcgatccggt cgaggagcgc ggcgccgagt ccgcgcccac gatgctcggg atgcaccaca
     1861 ccccacgact tgaccttgcc ctcgccgtcg ggggagacgt tcgcgtacgc gacgaccgcg
     1921 ccgttcgggt cggtgaccac ccacgcgtcc tcggacaggt cgacccccgg ggacgaccac
     1981 tggtcacgga tgagatcggc gtcggtctcc gatacaccgg tgtcggcgag gtcggtggcg
     2041 accagcatct cggtcactac ctcgacgtcg tcgaacgtgg ggggctggac ctcgtagccg
     2101 tcggcgagaa gcagcatgct cggagcctac gggttgcggt cgcgagcggg tcgccgatga
     2161 tgggttcgtg acgacgagcg ttcgaccgcc cggtcccgac ctgtccaggc tcgcggcgct
     2221 gcgggcgttc cgcaaggatc cgctcgggct cctcgaacgc ctggccgcct acggcgacgt
     2281 cgttcggatc gacgtgccgg gctccgccgc gttcctgctc aaccatccgg atctggtgca
     2341 cgaggtgctc gtcgccgagc accgttcgtt ccacaagggg ccgacgatcc aagccgcgaa
     2401 gttgctgctc ggggaatcgc tgctcacgag cgaggacgac gtccaccgcc ggcagcgccg
     2461 gctgatccag ccgatgttcc accacgagcg catcgcgtcc tacgcggcgt cgatggtccg
     2521 gttcgccgag cgcg
//
LOCUS       DASUYD010000104         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_715038, whole genome shotgun sequence.
ACCESSION   DASUYD010000104 DASUYD010000000
VERSION     DASUYD010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715038"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VF195_05665"
     CDS             <1..170
                     /locus_tag="VF195_05665"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX6580341.1"
                     /translation="GGLGFVEAGLTAMLVLAGVPAGDASLAVLAYRLVSYWLPLVAGA
                     LAILAYQRRFA"
     gene            321..542
                     /locus_tag="VF195_05670"
     CDS             321..542
                     /locus_tag="VF195_05670"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX6580342.1"
                     /translation="MSDDLVSVLWTSSLVEAEIAKGRLEAESIPVQLVGEVGDDPFPV
                     GPAELLVPSRFEEEARRILAQSEDGSDQS"
     gene            988..2082
                     /locus_tag="VF195_05675"
     CDS             988..2082
                     /locus_tag="VF195_05675"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX6580343.1"
                     /translation="MDHATHLGLDVHKDTIAVAILRPDQDAPEERVIPNTSEALRKLV
                     ARRRGSVVACYEAGPTGYETHRLLSSLGVPCDVIAPTLIPRRAGRRVKTDRLDARNLA
                     RLHRAGELTAIRVPTPEEEALRDLIRVREDLKDDRRKTMHRVKSFLLRQGRRYPGRGN
                     GWTDKFDLWVRGQRFTEPAADAAFRHYLAALDTRRAQLRALDVELEAAAGQPPIAGQV
                     ARLRCFRGIDTLSAVTIAAEVCDFHRFGSATSFMGFTGLVPSEYSSGASEHRGSITKA
                     GNAHLRRVLVEAAWSYRHRPAVGLELRRRSEGQPPEVLAYAWAAQCRLSSRFRQLAAR
                     REHNVAVVAVARELAGFIWGLMTDRIAVSA"
     gene            complement(2418..>2939)
                     /locus_tag="VF195_05680"
     CDS             complement(2418..>2939)
                     /locus_tag="VF195_05680"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580344.1"
                     /translation="VFLISLQLAFGVFGPNPVSDLIQGIIAYLPNLFAAVVILVIVAA
                     LAKVVTDLLAAALGEVSGGEWIARAAGTAILVIGVFAALNQLQIAPAIVNGLFYALLA
                     IIVGVAIVAFGGGGIQTARGYWERVGQRADAKASEIRRSAAPESAHQRDSDVVGIPEV
                     EQESPGKSPGWEA"
BASE COUNT          484 a          991 c          978 g          486 t
ORIGIN      
        1 cgggcgggct cggtttcgtc gaggcggggc tgacggccat gctcgtgctc gcgggggtcc
       61 cggcgggcga cgcgagcctc gccgtcctcg cgtaccggct cgtgtcgtac tggctgccgc
      121 tcgtcgcagg agcgctcgcg atcctcgcgt accaacggcg gttcgcctga tcgactcggc
      181 cccaccgagg gcgtcgctcc agcccgcccg gtcccgacag gccccgagat gctgtcatta
      241 gcccacgggt cgctgccctg ttcgttctgc ccgaacagcc ggaatcgcga ggcgaggacc
      301 cgacgtgcca tgatgagcgc gtgagcgatg acctcgtctc ggtgctgtgg acctcatctt
      361 tggtcgaggc ggagatcgcg aagggccggc tcgaagcgga gagcatcccc gtgcaactgg
      421 taggagaggt cggcgacgac ccgttcccgg taggcccggc cgaactgctc gttccatcga
      481 ggttcgagga ggaggcgcgc cgcatcctcg cgcagagcga agacggctcc gatcagtcct
      541 gagcatgggc ccgtgggcga gcgcgtagct gatcctcgaa tgcgacggtg tcaagacccg
      601 agcctcggcc gcgagacgat cacccagttg tggacacagt ggctggcgcg agccgaacgt
      661 ctcggccgtc cgaggccctc gatgccgcga gtacaggggt gtttggcggc tccccgtgga
      721 gcagcacctc gggccgattc cgctcgctcg cctcactcgt tgagacgtgg aggacgtggt
      781 cgtcgccgcg gaacgtgacg cctaggcctg gcaagggctc gaacctgtga ggctgggtca
      841 tcgagccgtt cacagtcgcg gaagagcgct tacagatcct caacggagtt tgaatagtca
      901 cgatgagacg gccggtcact ccgtgatgat tcgtggatca cgcgcggatg ggccgcgcga
      961 ggagccacac acggaggtgg accggccatg gaccacgcta cgcacctggg tctggacgtg
     1021 cacaaggaca cgatcgcggt cgccatcctg cggccggacc aggacgctcc cgaggagcgg
     1081 gtcatcccga acacctccga ggcgctacgc aagctagttg cccgccgacg gggcagcgtc
     1141 gtcgcctgct acgaggccgg tcccacgggc tacgagacgc accgcctcct gagctcgctc
     1201 ggcgttccgt gcgacgtcat cgccccgacg ctcatccctc gccgggcagg caggcgggtg
     1261 aagaccgacc gcttagacgc ccgcaacctg gctcgcctgc atcgggcggg tgagctcacc
     1321 gccatccgcg tacccacgcc agaagaggag gcgcttcgcg atctcatccg ggtccgcgag
     1381 gacctgaagg acgatcgccg aaagacgatg cacagggtga agagtttcct gcttcggcag
     1441 ggccgccgat accccggcag gggcaacggc tggaccgaca agttcgacct ctgggtccgg
     1501 gggcagcgct tcaccgagcc cgccgccgat gcggcctttc gccactacct tgctgctttg
     1561 gacactcgcc gggcccagct gcgggccctg gacgtcgaac tcgaagccgc ggcggggcag
     1621 ccaccaatcg cgggccaggt ggcgaggctc cggtgcttcc ggggcatcga cacgctgtcc
     1681 gcggtgacca ttgcggccga ggtctgcgac tttcaccgct tcggatccgc cacctcgttc
     1741 atgggcttca ccggcctcgt gccctccgag tactcgagcg gagcgagcga gcatcgggga
     1801 tcgatcacca aggccggcaa cgcgcatctt cgccgggtcc tggtcgaggc cgcctggagt
     1861 taccgtcacc gccccgccgt tgggctcgag ctccgccggc gaagcgaggg gcagcctccg
     1921 gaggtcctcg cctacgcgtg ggccgctcag tgtcggctgt cctcgcgctt tcgccagctc
     1981 gccgcgcgca gggagcacaa cgtggccgtc gtcgccgtgg caagggagct ggccgggttc
     2041 atctgggggc tgatgaccga ccggatcgcc gtgtcggcat gaaggcgtgg gagcgggcgg
     2101 gcgtgcaccg cggaaggatc ctcgcggacg ctatgcggct tcagatccgc gttcttagtc
     2161 cgaggcagct tccgacgaac ccgtgatgtg cggttccgat ccgcgaatac gagagtggtc
     2221 gatcgtcgct gccacctgtc cgcccgctcc cgcctctctc caatcgcggc cgccgtgagg
     2281 gcttgacttg acatcacgtc tcatacgagt gtccccttag gtccacgttt caaggtctta
     2341 ttcgacgtcg gccgagggaa cacaggaccg acttctcgct catcgcatcc cttgctccgt
     2401 cgatgagcgc gcttcgatca ggcctcccag cccggtgatt tgcctggtga ttcctgttca
     2461 acttccggga tccctacgac atcgctatcg cgttggtgag cggactccgg ggcagcactt
     2521 cgccggatct cggacgcttt cgcgtctgcg cgctgaccga cacgttccca gtatccacgc
     2581 gcggtctgga tgccgccccc accgaaagca acgatcgcca caccaacgat gatggccagc
     2641 agagcgtaga agagaccgtt gacgatcgcc ggcgcgatct gcagctgatt cagggccgcg
     2701 aagaccccga tgacgaggat agccgttccc gccgcacggg cgatccattc gcctcccgag
     2761 acttctccga gcgcggcagc cagcaggtca gtcacgacct tggccagcgc tgcgacgatc
     2821 accagaatga caacggctgc aaagagattc ggcaggtacg caatgatgcc ttggatcagg
     2881 tcactgaccg ggttgggacc gaacacaccg aaggcgagct gcagcgaaat caggaatac
//
LOCUS       DASUYD010000105         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_496587, whole genome shotgun sequence.
ACCESSION   DASUYD010000105 DASUYD010000000
VERSION     DASUYD010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496587"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            297..1625
                     /locus_tag="VF195_05685"
     CDS             297..1625
                     /locus_tag="VF195_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580345.1"
                     /translation="MRAIDLAYHVRAGELSLRTGDLLLIDPFTFTSGARPWLNQQWVA
                     QLVFASAHRLLGWAGVAITYAGAMASGFGLLYRSCRRAKATPRTAAVLALLGFMVASG
                     SLAARPQALAVPLFTGTWLLLARRHRWMWGVPVLAVIWANVHGSFVLAPGLVAFTLCD
                     DLLERKDPRPTLLLLLATAGATFATPFGGSVWTYALDLVGNETIRSSVAEWRPPSPLS
                     LSGGPFWASAVAVAIVGISKRREIRLIDVARLLVFFALGVPALRGTLWWALVAPPIVA
                     GWFPARDPVDPAKSVESRWVPTLVATAIVLMTLAFALNLRAGTDPVTGGPARLAADAP
                     EDLAIAARKILPPGSRLLVFQPFASWFEYSLPEDPVMVDSRIELFPSTVWRDYSLATR
                     ADRGWERVLDRYGIRGVVLPPGEPLGVALAHAPGWTLGAQIPSGSIFVRS"
     gene            complement(1632..2339)
                     /locus_tag="VF195_05690"
     CDS             complement(1632..2339)
                     /locus_tag="VF195_05690"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580346.1"
                     /translation="MVVSESSIDARGRRTSARHDVVIVEPFPVVRAGIGLIVEASTGL
                     DVAGTSASAAEAVAVIERLDRTQLVVLVSMGLAGESDAYWLIRALRERFPGLVILGLG
                     ANADPNTVSRALFTGADGFIDKNVEVEEFLEAIRNASEEEMVIAGPAAASVGTIAEGI
                     ERRRSQTFRLTRREREVLAVAAEGLTAREIADRLGVRERTVTTHLARIYGKLGVGNRL
                     AALRLAARSGLFDAPTR"
BASE COUNT          359 a          884 c          781 g          461 t
ORIGIN      
        1 gtcctcgggg cgtacgcccc gttggcggag tcaatccgcc agcgagcacg atatggcgcc
       61 ccatccctcc agggaatccc gtattccact ggagttatct acgtgcttct acgtacgtat
      121 ctccccaacc gaacgagaag acgccggatg gtaccccgta ggatgccgcc gtggaaaccc
      181 ccaaaggggc agatttctgt gctcactttc ggctggcctc cgtaggccga agggccgacc
      241 ccccgtgggc cgtcctctcc tggtctctcc cggcgctcgc tgcgctccta gcgaagatgc
      301 gcgccatcga cctcgcctat cacgtgcggg caggcgagct cagcctccga acgggagatc
      361 ttctcctcat cgacccgttc acgttcacga gcggcgcccg accctggctg aaccagcaat
      421 gggtcgccca attggtgttc gcttcggcgc atcggctgct cggttgggca ggggtcgcga
      481 tcacgtatgc cggcgccatg gcatccggat tcggactcct ctatcggagc tgcaggaggg
      541 cgaaggcgac cccgcgaacg gcggcggtgc tcgcgctcct cggattcatg gttgcgtccg
      601 gatcgctcgc cgctcgaccg caggcgctgg cggtaccgct cttcaccggc acctggctct
      661 tgctggcgag acgacatcgc tggatgtggg gtgttccggt cctcgccgtg atctgggcca
      721 acgtccacgg gagcttcgtc ctcgcacccg gcctggtcgc tttcacccta tgcgacgatc
      781 tgctcgaacg gaaggatccg cgtcccaccc tcctgctgct cctcgccaca gcgggggcta
      841 ccttcgccac cccgttcggc ggctccgtct ggacgtacgc cctcgatctg gtaggcaacg
      901 aaacgatccg gagttcggtc gcagagtgga gaccaccatc ccccctctcc ctcagcggcg
      961 ggcccttctg ggcctccgcc gtcgccgtgg ccatcgtcgg catctcgaag cgacgggaga
     1021 tccgtctcat cgacgtcgcc cgtctgctcg tgttcttcgc actcggagtg cccgctctcc
     1081 gaggcacgct gtggtgggcc ctcgtcgcac ctccgatcgt ggccggctgg ttcccggcac
     1141 gggatcccgt cgatcctgcc aagagcgttg agtcgaggtg ggttccgacg ctcgtcgcga
     1201 ccgcgatcgt cctgatgact ctcgcattcg ccctgaacct tcgagccggg acggatccgg
     1261 tgacgggcgg tccggcgcgg ctggccgccg acgcgccgga agatctcgcg atcgcggccc
     1321 ggaagatcct cccccccggg tccaggctct tggtcttcca accgttcgcc tcttggttcg
     1381 agtactccct gcccgaggat ccggtgatgg tggattcccg gatcgagctg ttcccaagca
     1441 cggtgtggcg cgactactcg ctcgctacgc gtgccgatcg ggggtgggaa agggtcctcg
     1501 accgatacgg gatccggggc gtggtgctgc ccccaggaga gccgctcggc gtggcactgg
     1561 ctcatgcccc tgggtggaca ctcggggcgc agatcccctc aggttcgatc ttcgttcggt
     1621 cctgaagcta ctcagcgggt tggagcgtcg aagagtcccg acctcgccgc gagccgcagc
     1681 gcagcgagcc tgttcccgac tccgagcttc ccgtagatcc gtgcgaggtg ggtcgtgacg
     1741 gtccgctcgc gcacgcccag ccggtcagcg atctcccgag cggtcaggcc ctccgccgcg
     1801 acggccagga cctctcgctc gcgtcgggtg agacggaacg tctggctcct ccggcgttcg
     1861 atcccttccg cgatcgttcc gacagaggcc gcggcgggcc cggcgatcac catctcctct
     1921 tcgctcgcgt tacggatcgc ctcgaggaat tcctcgacct ccacgttctt gtcgatgaat
     1981 ccgtccgcgc ccgtgaacag cgcacgtgac acggtgttcg ggtcggcgtt cgcacccagt
     2041 cccaggatga cgagcccggg gaagcgttcg cggagcgcgc ggatcagcca gtaggcgtcc
     2101 gactcgcccg ccaatcccat cgataccagg acgacgagct gggttcgatc gaggcgttcg
     2161 atgacggcga ccgcttccgc ggcgctcgcg gatgtaccgg cgacatcgag ccccgtactc
     2221 gcttcgacga tcaggccgat gcccgcgcgg acgacgggga acggctccac gatcaccacg
     2281 tcgtggcgag cggaagtcct cctccccctc gcgtcgatcg acgactcact caccaccatc
     2341 gggaccacct tcgccgttcg gagtagtgga tcgcaacgct acaggtcgac caatccgagt
     2401 ctcgcagcct gggagaccgc ctgtacacgc gtgttcactc cgagccttcg gtacagggtc
     2461 gccacgtgtg tttccacggt ccgag
//
LOCUS       DASUYD010000106         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1157413, whole genome shotgun sequence.
ACCESSION   DASUYD010000106 DASUYD010000000
VERSION     DASUYD010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157413"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF195_05695"
     CDS             complement(<1..870)
                     /locus_tag="VF195_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6580347.1"
                     /translation="MQTPRLIGDRYRLGDVLGRSLMSEVRRGEDTLLGRPVAVKLLLD
                     YGDPRSVALFQQEAQILARLQHPNVISVFDAGVDGGDRFIVMELVEGPTLRELLETEG
                     PLAPERAGEIASRLASALGFAHGKAVIHRDVKPSNVLLPADGGVKLADMGIARLLSPE
                     ALTATLSVRGTALYISPEQVRGDRVDPRTDLYSLGCVLFEMLTGRTPFEGDLAALSYA
                     HTHTPAPRVRSIDPAVPAAMDELVAAMLEKDPARRPSTGEEVERSLAAAMRHDAVVPT
                     APTERVQPPPSPTP"
     gene            complement(886..1764)
                     /locus_tag="VF195_05700"
     CDS             complement(886..1764)
                     /locus_tag="VF195_05700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX6580348.1"
                     /translation="MKTGPEAGRRVELDLEVAIGRQDGDLVVEDPEVSRRHAVLRRSG
                     GSVVVEDLDSTNGTFVNGERIRSPITVGPGDQVRVGRTMLEIEPDQRADDTIVSTPPR
                     PDQIRTAEAPPSGGVLAEEQQDATQPLPSRALESGSRPARSNRRWLGVGAAALVVLLG
                     VIAYVALVDRSAENDFASRANEACAAVQRPEGGVDLSRTPTRGELQGARNVRLQALGA
                     IRVLERPEQDAVSVSRFLSAFGETNASITALERAIGGDNRAVVRAVGKLRGDVRDERE
                     LAAKAGIAGCGGLAIR"
     gene            1870..2196
                     /locus_tag="VF195_05705"
     CDS             1870..2196
                     /locus_tag="VF195_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580349.1"
                     /translation="MVQGRCSLAEGSSSLALYEREAAAQDAGVSSEGSGFRGVSFHYI
                     VTSSEAVDEDMSKAMAAVAGVVKEAAGTQRGHFGYFSDPNGYPGRSPRRTRKAEAVIG
                     ALVTTR"
     gene            2235..2765
                     /locus_tag="VF195_05710"
     CDS             2235..2765
                     /locus_tag="VF195_05710"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011978320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580350.1"
                     /translation="MREQILVAPASADDLEAISGIYDHYVRTTAISFDVEPPTTAWRR
                     EWFARFDDRGRHRLLVARDDGEAIGYAASVPYRPREAYGPSVETSVYVSPTHLGRGVG
                     CALYGALLSELEREDVHRAYAGIAMPNPASVRLHERCGFRRAGYFTEQGRKFGRYWDV
                     AWFERGFPEVASATSG"
     gene            2778..>2983
                     /locus_tag="VF195_05715"
     CDS             2778..>2983
                     /locus_tag="VF195_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580351.1"
                     /translation="MVDGLEGRIDELYGLPLERFTAERDALAKELASAGDRDGAARVK
                     ALRKPVVAAWAVNLLAREDPDGVK"
BASE COUNT          454 a         1015 c         1037 g          477 t
ORIGIN      
        1 aggcgtcggc gagggtggcg gttgcactcg ctccgtcggc gcggtcggca cgacggcgtc
       61 gtgcctcatg gcggcggcaa gagacctctc gacctcctct ccggttgacg ggcgcctggc
      121 gggatccttc tcgagcatcg cggcgacgag ttcgtccatc gcggccggaa cggcggggtc
      181 gatcgagcga acacgaggcg cgggggtgtg ggtatgcgca tacgagagtg ccgcaaggtc
      241 tccctcgaac ggcgtccggc cggtgagcat ctcgaacagc acacagccca gggagtacag
      301 gtccgttcga gggtcgacac ggtcacctcg cacctgctcc ggcgagatgt acagagcggt
      361 gccccgcacg ctcagggtcg cggtgagggc ctcgggggac aggagtcggg cgatccccat
      421 gtccgcaagc ttcaccccgc catcggccgg gagcagcacg ttcgacggtt tgacgtcccg
      481 gtggatgacg gcctttccgt gggcgaaccc cagggcggag gccaggcggc ttgcgatctc
      541 gcctgctcgt tcgggcgcga gcgggccctc cgtctcgagc agctcccgga gcgtcggtcc
      601 ctcgacgagc tccatcacga tgaaacggtc accgccgtcg accccggcgt cgaacacgct
      661 gatcacgttc gggtgttgga ggcgggcgag gatctgggcc tcctgctgga acagggcgac
      721 cgagcgggga tccccgtagt cgaggagcag cttcacggct actggccgcc ccaacagggt
      781 gtcctcgccc cggcggacct cggacatcag gctccgtccg agcacgtccc ccaggcggta
      841 gcggtcgccg atgagtcgag gcgtctggat gtcgggtcgt ccttcctaac ggatcgcaag
      901 acctccacat cccgcgatac cggccttcgc cgcaagttcc cgttcgtctc tgacgtcccc
      961 tcggagcttt ccgacggcgc ggacgaccgc gcggttgtcg ccaccgatcg ctcgttccaa
     1021 ggcggtgatg gatgcgttcg tctccccgaa ggcggacagg aacctgctca ccgacacggc
     1081 atcttgttcg ggccgctcga gcacgcggat cgccccgagc gcctggagtc gcacgttccg
     1141 ggcaccctgg agttctccgc gcgttggagt ccggctgaga tccacgcctc cttccggccg
     1201 ctgcacggcc gcgcacgctt cgttggctcg ggaggcgaag tcgttctcgg ccgaacggtc
     1261 aacgagtgcc acgtaggcga tcacccccaa gagcacgacc agggctgcgg ccccgacccc
     1321 gagccacctc ctgttcgagc gcgcgggtcg cgaaccgctt tcgagcgccc gggacggcaa
     1381 gggctgggtc gcgtcttgtt gctcctccgc gaggacacca ccggacggtg gcgcctcggc
     1441 ggttcggatc tgatccggcc gcgggggtgt cgacacgatc gtgtcgtcag ctcgctggtc
     1501 cggctcgatc tcgagcatgg tccgtccgac cctgacctgg tcaccgggac ccaccgtgat
     1561 ggggcttcgg atcctctccc cgttgacgaa ggtgccgttg gtcgagtcga ggtcttcgac
     1621 gaccaccgat ccgcccgagc gtcgtagcac cgcatggcgg cgcgacacct cgggatcctc
     1681 caccacgagg tcgccgtcct gacgaccgat ggccacctcg aggtcgagtt cgacccgcct
     1741 gcccgcctcc ggaccggtct tcaccacaac cgttggcaac cctcaccccc agggttggcg
     1801 aacgacacca ccacgacgat cgtgcggctc gttcgttatc ggtcgcgtgt cagcggacct
     1861 tgacgtcgga tggtacaagg caggtgttct ctcgctgagg gatcgtcgtc gctggccctg
     1921 tacgaacgcg aggcagcggc tcaagacgcc ggcgtctcct ccgagggatc tgggttccga
     1981 ggtgtctcgt tccactacat cgtcacctcg agcgaagccg tggacgagga catgagcaag
     2041 gccatggcgg ccgttgccgg cgtggtgaag gaagccgcag gcacccagcg gggacacttc
     2101 ggctacttca gtgacccgaa cggctaccct ggaagatcgc ctcgtcgtac tcggaaggcc
     2161 gaggcggtga tcggcgcact cgtaaccacg cggtaaccca tccacccgct cgcgcctgaa
     2221 aggatgggcg tgtggtgcgt gaacagatcc tggtcgcgcc ggcttcggcc gacgacctcg
     2281 aagccatctc gggcatctac gaccactacg ttaggacgac cgcgatctcg ttcgacgtcg
     2341 aaccgccgac gaccgcatgg cggcgggaat ggttcgcgag gttcgacgac cgaggacgcc
     2401 accgcctcct cgtcgctcgg gacgacgggg aggcgatcgg ctacgcggcg agcgtgccgt
     2461 accgcccgag ggaggcctat ggaccatctg tcgagaccag cgtgtacgtc tcgcccaccc
     2521 atctggggcg cggggtcggg tgcgcgctct acggcgcgct gctgtccgag ctcgaacgcg
     2581 aggacgtcca ccgcgcgtac gcggggatcg cgatgcccaa cccggcgtcg gtgcgcctcc
     2641 acgagcggtg cgggttccgc cgggccggct acttcaccga gcaggggcgc aagttcgggc
     2701 gatactggga cgtggcgtgg ttcgaacggg gtttcccgga ggtggcgtcc gcgacctccg
     2761 ggtgagaggt tggaggggtg gtcgacggac tcgaaggacg gatcgacgag ctgtacgggc
     2821 tcccgctcga gcgcttcacg gccgaacgcg acgcgctggc gaaggagctc gcgagcgccg
     2881 gcgatcgcga cggcgccgcc cgcgtcaagg cgctccgcaa gccggtcgtc gcggcctggg
     2941 cggtgaacct gctcgcccgg gaggacccgg acggcgtcaa gga
//
LOCUS       DASUYD010000107        21577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_275989, whole genome shotgun sequence.
ACCESSION   DASUYD010000107 DASUYD010000000
VERSION     DASUYD010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 21577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21577
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275989"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..690)
                     /locus_tag="VF195_05720"
     CDS             complement(334..690)
                     /locus_tag="VF195_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580352.1"
                     /translation="MEARRFVRESVGPPIPRRIIDDAVLLTSELVTNAVRHAGLDDQD
                     QIGVTVSVDPRVLRITVRDRGPGFDPEAVARRRSDEGGWGLDLLRRSSSRWGVDRGEA
                     GTDVWFEIDLPDRSKI"
     gene            843..1655
                     /locus_tag="VF195_05725"
     CDS             843..1655
                     /locus_tag="VF195_05725"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6580353.1"
                     /translation="MAGTSHATNARDGVRVYLDDGGGGGAPVLLLGGFLDPIELVRRA
                     PIVQALAGFADEFRLIFVDHRGHGRSDKPHDADAYAMPRRVADVVAVLDELQIRRAHL
                     LGISWGGRLCFGVGRHAPERVRSLISIGQHPYAIDRGGPLARVVGDALAASKQRGIEA
                     LVEAFEAIAGRYPDDVRATYLACDAEAMRAAWQAATTEGAVAEELGTWSLRCLVCAAA
                     DDADFFEDARRAASEIPDAEFVAIPGTDHLGVDTARVDPVLPAVLRTLRDAS"
     gene            complement(1668..3020)
                     /locus_tag="VF195_05730"
     CDS             complement(1668..3020)
                     /locus_tag="VF195_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEX6580354.1"
                     /translation="MGGGGDSQIPAGFTYLGQFVDHDLTFDKTSVMLGEDISPAELLQ
                     ARSPSLDLDSLYGAGPADPASEKFYEADGIHLKMGKTLAAEGLPGMEGFDLPRGAGTA
                     AAAKRKAIIPDPRNDENLAVAQTHLAMIRFHNRVVDTLPASVPPGLRFLKARAKVTRH
                     YQWMLRTDYLPRICASGVVSNVFNSGRKAFEVGVPATDVPTMPIEFSVAAFRLGHSMI
                     RRAYDWNNIFDNGSGTLEFLFTFSAGSGRLGGGSRLPSSWIADFRRLYDFNEAGRADL
                     AVPEAKFSRAMRIDTTLTNPLANLPGFPHGEDNLAFRNLTRASMVKLATGQQMATFLQ
                     NKGVSLTKLTTPQIRNGLNGVTLEGLTQAQRTALLADTPLWFYILREAELNQGRLKGV
                     GARIVAETFHRAIEGSTISIVRAPGWRPTLGPDNTTFRMVDLLLFAFEGKKNLLAPVT
                     "
     gene            3471..3758
                     /locus_tag="VF195_05735"
     CDS             3471..3758
                     /locus_tag="VF195_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013884316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HEX6580355.1"
                     /translation="MDDFRKLIKWTAIALAAAAIVEQLRRPSEERTWEGTVVGFVPYD
                     FRMPTIERARSRWWNPDEPRLFVPQVFGVGWTINFARLIGLLRDTARPSAA"
     gene            complement(4096..5430)
                     /locus_tag="VF195_05740"
     CDS             complement(4096..5430)
                     /locus_tag="VF195_05740"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580356.1"
                     /translation="MEIPETRYAKTADGVHIAYQIVGNGPVDFVYVGPWATHIEYRWE
                     LPRYASYLRRLASFSRLILFDKRGLGMSDPVPADEFPDLETRMDDIRAVMDEVDSRRA
                     VIYGASESGMLDLLFAATYPERTLALVIHGSYPSARWEPDAPWGWRADEFSERLRNIE
                     EMWGTQDFIRREFPSIAADAPLVRWFATYTRRSMSPGAAVTAELMEYDTDVRHVLPAI
                     HVPTLILHRIEDDPEANRYMAERISGAEYIALPGSEHIPYLGDQDRVLIEIERFVKGV
                     QADEASLDRVLATVLFTDIVGSTEKAVELGDSTWRTLVEEHHATVRRLLTRYRGTEVD
                     TAGDGFFASFDGPARAIRCAQAIKEAVSRLGLDIRAGLHTGECESIGGKVGGIAVVIG
                     SRVAALAEPGEVLVSQTVKDLVAGSGLTFEDAGERDLKGIPDRWRLYRVTDK"
     gene            complement(5569..5653)
                     /locus_tag="VF195_05745"
     tRNA            complement(5569..5653)
                     /locus_tag="VF195_05745"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5616..5618),aa:Leu,seq:tag)
     gene            complement(5895..7787)
                     /locus_tag="VF195_05750"
     CDS             complement(5895..7787)
                     /locus_tag="VF195_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580357.1"
                     /translation="MRRLTLAVGLATCVLVPSLLFLGRYVVDGTGVAPAGSDTSQHVW
                     RSEVVAENGLEALPAFDGRSQALNTNADRPGLPLIFSSLSAVTGADVRDLAYVLPAVL
                     AAAIALAAAALAGAVPGVPGWGMALAGVSTGASVQVALAANGYLDQLLVEPLLVMAGA
                     CVLRAAGGGPGRALGAAALVAAWLVHWQFALLFTVLLGVVSLASLPASLRDRRQGLPL
                     IETASGRVGTTVVGGAGLGIAALLFGTPGIPHAPIGLSRGSVDRHLSDQLARYRLPAA
                     VAAAMVGGTWLAVDGSEPSRRRAGWLLVPWALVPAAAALAFAAGRTVPLQRALSFSLA
                     IPVLGALGLVAAVLWTRGRFGSVAAGAVGLLAVMALIFSVTFAWETWRTRKPWSQDQA
                     LAEFRALGSYLIEAGRPAIVVVDEGSGQEKGAEGEFGTVPVMRRIRAELPAHLAMRTT
                     VYLGDAEFLTEGRPTLRPEIVGFDEVSRETWLAARVLLADDPTIVVLRSHLNGFAEAI
                     DAHPEWRTNGWMAVVAGPPPPIDPPVVPERPSSSALFAWWVSSLAVIALPGAGWSARF
                     GGGPFGLRMALAPATGLAALVFIGLLVERLGVRTGGGGGMITAIVIAASGVLAAIIRR
                     PAERSA"
     gene            complement(7784..7912)
                     /locus_tag="VF195_05755"
     CDS             complement(7784..7912)
                     /locus_tag="VF195_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580358.1"
                     /translation="MGERRRGDAAASSPCVYAANHLPGSWRSVPEGVLPTLLEEEG"
     gene            7993..8754
                     /locus_tag="VF195_05760"
     CDS             7993..8754
                     /locus_tag="VF195_05760"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX6580359.1"
                     /translation="MLRRLLLLVSAVLLAACATSALAPVSQPSLPRSRPRPPADVGVV
                     KMLARGGTLAVASRNDVLAVHAAPGTERPFQHLAADNPWGQPLRLLVVRDTIEESEIW
                     LRIQLPIWPNGQEGWVKASDVRLAEATERVVVDLSERRLVRLREGRRVARLRVAIGKA
                     ATPTPPGRYFVWAKIGTGRPSGPYGSYILGLSGFSEAIEPWTEWPGQPRLAIHGTHDP
                     ADAGHAVSNGCIRVLNRLLGVLHDVPMGTPVLIRP"
     gene            8844..12338
                     /locus_tag="VF195_05765"
     CDS             8844..12338
                     /locus_tag="VF195_05765"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580360.1"
                     /translation="MMRCGACGAHNPDGARFCNACGERLGVSGAGPIAAGSGERRVVT
                     MLFCDVRGSTVMAEELDPEDWTDVMNEAFAYLIEPVVRNEGTVARLMGDAILAFFGAP
                     TAHEDDPQRAVMAGLEIVSSIAALREKVARERGLDLNVRVGINTGAVVVGDVGSELKQ
                     EYSAMGDAVNVAARMEQTAEPGSVQITEETYRLVADLFEVEPLGGVELKGKRRPVDSY
                     RVLGRLDAPWTTRAARRMDAPLVGREEEMAAIRAAFDRAHEGRGSVLLLVGDPGIGKS
                     RLIEEANAIWAIAEPEDDRRWDFWGCVPFDTMQPYAQYRRLMKERANVKESDPAETVR
                     AKIGDLMESIALEGWEERSERVARALLGVEREDEPHLEGEEFQGAATELVVGSTLAQG
                     GRRLIVFEDLHWCDHASLELVRATTELVAENPIVVLASFRPDREAISWEFKEWVETDL
                     AAHATVLELDSLSAEQSDELIGAFLPIPEMTGAERQRILRNTEGNPLFMQEVARALID
                     GGVVERGDDGWRLTADATEIVIPDTIQSLITVGLDRLPESTRRTVQTAAVIGRTFQEE
                     LLVAVAGRADVRDDLRELVRRDLIRVTGDGSPAEFTFRHALTQEAAYGTLLAKHRRAT
                     HRRVAEVLEEMARERLDEVASQLVRHFSEAGEDDSTVTYAAKAGDAAARLYANAEAEA
                     HYRTGLDAARRIAAPSPLLRSMYERRGSALELIGRYDDAIENYEEMRAEAVARGDDAM
                     ELSANSSIALLFATATPKFDPELGRRLSEELVVMARRIGDRSSEARALWNIVVANVYG
                     GGDAARAVEAGEASLVIARELGEREQLAFTLNDVTRAYLSLGDFSTAAHRHAEARRLW
                     EELDNRPMLGENLTVGSSMHLFRGDYAAALADSHEAITVSESIGNAWGQSHALITVYR
                     VELERGELGAAIASIQRCLDLGEQGGFSYAAIATRADLSRVMTYLGDGERGLALAEES
                     LAIARDRVPPALSIARVSEALALISLGRRDEARAPLDEVDLMMLPEPDRTFLRVASGA
                     ARARLALLDGEVEEAEAVARSVIEELRASEVEILVAGAMVVLARALLAADRLDEAGHE
                     LADAIARAKGLGERRVLWEALALSADLHARRGAQREAADLRRRARAIVDGIAAGLPDA
                     DLRRRFLVRDDVRALEGG"
     gene            12340..12819
                     /locus_tag="VF195_05770"
     CDS             12340..12819
                     /locus_tag="VF195_05770"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006594087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6580361.1"
                     /translation="MTEPVVDKPEFPREYGNPTQRLDWAEVERKLESASVYWMASTRP
                     DGRPHVVPRDGTWLDGGLYYGGSPETVHARNIENNPSVAVHIGDGQEAIIVEGEVVIE
                     KPTQEMAERLSDASSAKYPQYGQMDPSIYMGGVSVLRPRRVLAWTSFTENATRFRFA"
     gene            complement(12824..12900)
                     /locus_tag="VF195_05775"
     tRNA            complement(12824..12900)
                     /locus_tag="VF195_05775"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12864..12866),aa:Lys,seq:ctt)
     gene            13035..13208
                     /locus_tag="VF195_05780"
     CDS             13035..13208
                     /locus_tag="VF195_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580362.1"
                     /translation="MSMTPEHHHHEDEVTPVRDPEGSGGLVGYAAIKYIAIVAIVLAI
                     LVFLALYLIPALT"
     gene            13244..13411
                     /locus_tag="VF195_05785"
     CDS             13244..13411
                     /locus_tag="VF195_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007464364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPGG-motif small membrane protein"
                     /protein_id="HEX6580363.1"
                     /translation="MAQAKEQTTDTILWIIAAVLVIAGIFAIVRSQVIWGIVLIVLGL
                     LVGPGGVSLLD"
     gene            complement(13474..13550)
                     /locus_tag="VF195_05790"
     tRNA            complement(13474..13550)
                     /locus_tag="VF195_05790"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13514..13516),aa:His,seq:gtg)
     gene            13655..15034
                     /locus_tag="VF195_05795"
     CDS             13655..15034
                     /locus_tag="VF195_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6580364.1"
                     /translation="MAVKETIDFDLDLIDRLIADEEATLEPKHRASIEYRRVAERTVA
                     GGVASSWQASPPHAIFIDRGEGSHIWDLDGNEYADYHLGYGAMVIGHAHPKVVEAIEK
                     RARLGTHFAQPTAQLHEIGENVAERFGLPLWRFCNSGTEATLEAVRLMRANTGRDLIV
                     KIEGTYHGHHDSLMFSVAPDPELIGAREHPVTVPQAMGIPQAFAELVRVVPFNDLAEA
                     ERAFTENEGRVAGMIVEPAMMNCGVVLPQPGYLQGLLDLCHRHGAYLAFDEVKTGGTI
                     AWGGAVEAFGVRPDIVTLAKAFGGGVPCGAIGATEELFGPVLRGEHDIAGTFNGNPLT
                     MAAARATLLEVLTPNAYERLHALDGALKDGIRPIIDRHRLPAYVTGIGAKGSVTYSGR
                     EVREYRDTIGIDERITYLAWLMQQNRGVFKSPWAKMETWTLSIVHSDDDVRRYVENFE
                     AFAAAVSGT"
     gene            complement(15023..15811)
                     /locus_tag="VF195_05800"
     CDS             complement(15023..15811)
                     /locus_tag="VF195_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580365.1"
                     /translation="MTVAALGWAPAQALAADGPIDPLLGSVAETTSGAADLVDPVQGT
                     VDQAAETVSSTADTLSDPLPDPVDEVVDTTSDTVDGVSGTTSGTVDPVSDATSGTVDA
                     VAGSTTDVVGGVTGAIGTTDSGLSDPSLPTGASVGGEPGASSAGSSVSDGTGEASSTR
                     AMESGPSGGAALLEAREGAVPASDIGTTPCVTVTPTTCETTSQTAQDSLAGRVAEIIR
                     KLLAFTGWGALPWIVAALVLSIVGIAAVRETGRRPRYSGSASGS"
     gene            complement(15999..16955)
                     /locus_tag="VF195_05805"
     CDS             complement(15999..16955)
                     /locus_tag="VF195_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580366.1"
                     /translation="MSKELLALSPAAPVGRRARSRTSSRKERRSARFERWRERRVYRL
                     TDRLEVRRDRLADRREHLFKNGHRGGLWTRIRGRLLSGAERLELLDELLLIRWNRFLL
                     HLSARWERLPGPASRGGAVAAAGVSISLVLAMVVMLPLWNAARIDSGPSPIVLPSRTE
                     LDIGSTQQAPAPTSEAITSEDYTNDGLGYRFSYPSDWAIEASSQATVLSDTADQVVVT
                     FLRAPPGRLAPASDQLLQRITATFTGAETLATETARTEQGYPSRSVGGTARDAVGTSI
                     RFVVITIGGPDGNRAIVVRFPTDPDPDDLDAVVQVIGSFRLV"
     gene            17148..17630
                     /locus_tag="VF195_05810"
     CDS             17148..17630
                     /locus_tag="VF195_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006672866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEX6580367.1"
                     /translation="MTRSSGAEVRKKAEHADARLRPFLARVGIPLLRISVGVVFLWFG
                     LLKIFDVSPVSGLVAKTIYWFDPDVVVPALGAVEVFVGLCLLVGRLMRLALPLLVLQM
                     AGTFLVLVLLPEIAFRDGNPLLLTVEGEFVVKNLVLFSAALVIGSRLAPLGEGGTGSA
                     "
     gene            17677..19038
                     /locus_tag="VF195_05815"
     CDS             17677..19038
                     /locus_tag="VF195_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX6580368.1"
                     /translation="MDIDLDWVEELTKRETEALDRRHARSLSYREEAKRYLPGGVSSS
                     WQSWPPHPIYVRRGEGAHVWDIDGNEYVDLHNGYGVMVMGHAHPKVVEAVQRRVAEGT
                     HFAQPTEEALPVAENLAERYRLPQWRFGNSGTEATLDACRISRALTGRSKILKIEGTY
                     HGHHDSLMVSVFPSEEQAGSRDRPVAVPQTKGLPKEFVDLVVNVPFNDLVAAKGAFAE
                     HPGEFACMIVEPAMTNCGLVLPDAGYLAGLKELCRGDGALFVFDEVKTGFTLAWGGAV
                     EAFGVVPDLVCLAKALGAGLPCGAIGGTDEAMGMVIRGELDQQGTFNGNPLTMAAAKA
                     NLLEVMTPEAYGRFDELNSVLGRSLEACTKHGLPANVKVLGAKGSVEFRDQPIHEYRD
                     LWDIDDRVPQLAWLWQLNRGVFKSPGSKWESWTTSIVHSDADVQRYVDNLEEFAATVT
                     TGT"
     gene            19081..19575
                     /gene="purE"
                     /locus_tag="VF195_05820"
     CDS             19081..19575
                     /gene="purE"
                     /locus_tag="VF195_05820"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013331286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEX6580369.1"
                     /translation="MDEPQVGVIMGSRSDWETMKHCCDTLEDLGIRFESKVVSAHRTP
                     ELMVEYAKTAADRGLRVIVAGAGGAAHLPGMTAANTHLPVLGVPIESKALKGMDSLLS
                     IVQMPAGVPVGSLAIGVSGAVNAGLLAAEIVALEDEHVREQLLKFRETQTQNVVAHPD
                     PDER"
     gene            19572..20666
                     /locus_tag="VF195_05825"
     CDS             19572..20666
                     /locus_tag="VF195_05825"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX6580370.1"
                     /translation="MTRVGIVGGGQLGMMLAEAGARIGIGIVTLDPAADAPATRVAPS
                     IVAAYDDEAALAELASVSDVVTYEFENVPVASARFLAERVAVSPPLESLEFAQDRLLE
                     KTLFEEVGLAVAPFAPVSSTEDLAEAMDLVGFPSILKTRRLGYDGKGQARLERPDQGG
                     DAWREVGQAPSILEALVPFDRELSILGARGRDGEVVFYPLVENVHRDGILRTSTPATS
                     VGVALASKAEDLAFAVMERLRHVGVLAIELFETGEGLVGNEMAPRVHNSGHWTIEGAA
                     TSQFENHLRAVSGIELGPTTRTAYSAMVNLIGSEPPDEAFPADPGVHVHRYGKHARPG
                     RKLGHVTIVEDDADTLIQVLREVTRTADNS"
     gene            20668..20937
                     /locus_tag="VF195_05830"
     CDS             20668..20937
                     /locus_tag="VF195_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580371.1"
                     /translation="MAYLTCPDCRMPSPVEDSAISYRCFSCAAEIIFCACHRCEYDQA
                     IPSRWFGAFTCGRCDSKVDIPRQRFYSTSVKASRVKGYGYTYPRF"
     gene            complement(20965..21090)
                     /locus_tag="VF195_05835"
     CDS             complement(20965..21090)
                     /locus_tag="VF195_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580372.1"
                     /translation="MGDDRNILWSEILASAEANDTASAEAVRNIEAMAEQMGATA"
BASE COUNT         3442 a         7318 c         7488 g         3329 t
ORIGIN      
        1 cagcggtctc gtggagcggt cccgcagacc ggcctcgccc tcttcccgga accggccaac
       61 ccacttcgag gccgtctgcc tgctcacgtt cgccgccctc gccgcggcgg tgaccgtcca
      121 ggcctgttcg gtcacccgca acgcgatcgt gaggcgtgag tgaaccgaga gccgagcgct
      181 gccatgctga cccatgaggg ccctcccttc tcgttcgtgg cttcgacacc tcgaaccgaa
      241 gggcagggcc ccttacatgt caacgatgtt ggtgggaagt agtgcctagc cggacagatc
      301 cgaggtcgtc gtcgtcgtga acgaggccgg cgctcagatc ttcgacctgt ccgggagatc
      361 gatctcgaac cagacgtccg tgccggcctc cccccgatcg acaccccatc gggaggacga
      421 tcgccggagg agatcgagcc cccagccacc ctcatccgat cgtcggcggg ccaccgcctc
      481 cggatcgaag ccgggtcccc gatcccgaac cgtgatccgg aggacccgtg ggtcgaccga
      541 cacggtgacc ccgatctggt cctgatcgtc caggcccgca tgccggacgg cgttcgtgac
      601 cagctcactg gtgaggagga cggcgtcgtc gatgatccgt ctcgggatcg gcggccccac
      661 gctctcccga acgaatcgac gcgcctccac gaaggctatg gcgcgcgacg atacgacccg
      721 ggaactggtg gatacaggcc tccttcccgt gaggtcggcg gcagcggtgc gggtgaccgc
      781 tcctgctgcg ccttaccctt ccggcgagga acccgaacat cgcagagtga aggatcgaga
      841 cggtggcagg gacgtcacac gcgacgaacg ctcgcgacgg tgtccgcgtg tacctcgatg
      901 acggcggcgg cggcggcgct cctgttctgc tcctcggtgg gttcctcgat ccgatcgagt
      961 tggtccggcg tgcgccgatc gtgcaggccc ttgccgggtt cgccgatgag ttccggctca
     1021 tcttcgtcga ccatcggggt cacgggcgga gcgacaagcc acacgatgcc gatgcgtacg
     1081 cgatgcctcg tcgcgtcgcg gacgtcgtcg ccgtgctcga cgagctgcag atccggcggg
     1141 cgcatctcct cggcatctcg tggggcggac ggctctgctt cggggtcggc cggcacgcac
     1201 ccgagcgcgt tcgctccctg atctccatcg gacagcatcc gtacgcgatc gatcgcggcg
     1261 gtcccctcgc gcgcgtcgtc ggggacgcgt tggcggcgtc gaagcagcga gggatcgaag
     1321 ccctcgtcga ggccttcgag gccatcgccg ggcgctaccc ggatgacgtg cgagcgacgt
     1381 acctcgcgtg tgacgcggaa gcgatgcggg ctgcgtggca ggcagcgacc accgagggag
     1441 ccgtcgcgga agagctcggg acatggagcc tgcgatgtct tgtctgcgcg gcggcggatg
     1501 acgcagactt cttcgaggac gctcgacgcg cggcttccga gatccctgac gccgagttcg
     1561 tggcgatccc agggaccgat cacctcggcg tggacacggc tcgcgtcgat ccggtccttc
     1621 ccgccgtgtt gcgaaccctc cgcgacgcga gctgagacgc gacgaaccta cgtcaccgga
     1681 gctagcaggt tcttcttccc ctcgaaggcg aacagcagca ggtcgaccat gcggaacgtg
     1741 gtgttgtcgg gaccgagcgt cgggcgccag ccgggcgccc gaacgatcga gatcgtgctg
     1801 ccctcgatgg ctcggtggaa ggtctccgcg acgatccggg cgccgacgcc cttcaaccgc
     1861 ccctggttca gctccgcctc gcggaggatg tagaaccaca gcggggtatc cgccaggagc
     1921 gccgtccgct gtgcctgggt gagcccttcg agcgtgacgc cgttcaggcc gttccggatc
     1981 tgcggggtcg tcagcttggt caggctgact cccttgttct gcaggaacgt ggccatctgc
     2041 tgaccggtgg ccagcttcac catgctcgct ctggtgaggt tgcggaacgc gaggttgtcc
     2101 tcgccgtggg ggaatccggg gaggttcgcc aacgggttcg tcagcgtcgt gtcgatccgc
     2161 atcgctcggc tgaacttcgc ctcgggaacg gcgaggtctg ccctgccggc ttcgttgaag
     2221 tcgtagagac gccggaagtc cgcgatccag ctgctcggaa gtcgcgaccc gccgcccaat
     2281 cttccgctcc cggcggagaa cgtgaagagg aactcgaggg tgcccgagcc gttgtcgaag
     2341 atgttgttcc agtcgtaggc cctgcggatc atcgagtgac cgagccggaa ggcggccacg
     2401 gagaactcga tcggcatcgt cgggacatcg gtggccggca cgccgacctc gaacgccttc
     2461 ctcccgctgt tgaacacgtt gctcacgacg cccgaggcgc agatcctcgg gaggtagtcg
     2521 gtgcggagca tccactggta gtgcctcgtg accttcgccc gcgccttcag gaagcgaagg
     2581 ccggggggta cggaggccgg aagcgtgtcc acgacccggt tatggaaacg gatcatcgcg
     2641 aggtgggtct gggcgacggc gaggttctca tcgttcctcg ggtccgggat gatcgccttc
     2701 cgcttggccg ccgccgcggt gccggcacct cgggggagat cgaacccctc catcccgggg
     2761 aggccctccg ccgcgagggt cttacccatc ttcaggtgga tgccgtccgc ctcgtagaac
     2821 ttctccgatg ccggatcggc cggaccagct ccgtagagcg agtcgaggtc gaggctgggc
     2881 gagcgcgcct ggaggagctc ggcgggggag atgtcctcgc cgagcatcac gctcgtcttg
     2941 tcgaaggtca gatcgtggtc gacgaactga ccgagatacg tgaacccggc cgggatctgg
     3001 gaatcgccgc caccgcccac ggcgatcgcc tctgacagcc tccgtcggct cggttccgga
     3061 agctggcgcc ccgctccgct cgggcccatg cgtgagaagc ggaagggcgg gacgagagcc
     3121 tcgtcggcgg gcgcgagggt gcgctctcgc cctccgacgg tctcaccgag aagcccttca
     3181 ccgatgacga agtagctctc cgaaccatgc ctctccacgg ctgccctccg ttcgtccgat
     3241 cgcgaaggtg atcacgatcg cccacactcg agatgccgac cgtaacgccg ggccccgagg
     3301 cgtcgtatcg gccatcgggg gtcgcatgga tgagctagtg cggaccgagt agtccacccc
     3361 ccaaagtacc ccggcgcttc tcgagaccgt cgcgatcaca tcggcggttg cagctgcgaa
     3421 accgactcct ggggcacgct cttgacgatc gtccgtggga ccatggctgg atggacgact
     3481 tccggaagct catcaagtgg acggcgatcg cccttgcggc cgcagcgatc gtggagcagc
     3541 tccgccgtcc ctccgaggag cgcacgtggg aggggaccgt tgtcggcttc gtcccgtatg
     3601 acttccggat gccgacgatc gagcgcgcgc gaagccgctg gtggaacccc gacgagcccc
     3661 ggctgttcgt tccgcaggtc ttcggcgtcg gctggacgat caacttcgcc aggttgatcg
     3721 gtcttctccg cgacacagcc cgcccaagcg cggcctagag gggccagcga cggatgtacc
     3781 gacgtggggt cgctcacggg tgggcactga ccgcgtgacc gacgcgcatc tcgaactctc
     3841 ctcgtggagc aggtgtgcgc gaggaggcca cctcgacccc tcgcagatgg agcctcgggg
     3901 agggttccgc ttgtggagtc catgcgggag gagggattcg aacccccacg ccctgccgag
     3961 cactggatcc taaggctact tgcgccgcgc acggagccgg gtcaactcgt cgtcctgtgc
     4021 cgtccactgt cgctccgtaa acgcgagtgt cgttcagtcg tgagcgatct gtgagcaagt
     4081 ggatggtggc gaccgtcact tgtctgtcac tcgatagagc cgccagcgat cagggatccc
     4141 cttcaggtcc cgttctcctg cgtcctcgaa cgtcaggccg cttcccgcca cgaggtcctt
     4201 caccgtctgc gacacgagca cttccccggg ctctgccaga gcggcgacgc gcgagccgat
     4261 caccacggcg atgccgccca ccttcccgcc gatcgactcg cactcgcccg tgtggagccc
     4321 ggctcggatg tcgagaccga gccgggaaac ggcttccttg atcgcctgcg cgcagcggat
     4381 cgcccgcgcg ggaccgtcga atgacgcgaa gaagccatca cccgccgtgt cgacctccgt
     4441 ccctcgatac cgagtgagca gacgtcgcac cgtcgcgtga tgctcctcga ccagcgtgcg
     4501 ccaggtgctg tctcccagct ctaccgcttt ctcggtggag ccaacgatgt cggtgaagag
     4561 caccgttgcg agtacccgat ccagggatgc ttcgtcggcc tgaacgcctt tgacgaagcg
     4621 ctcgatctcg atcagcactc tgtcctggtc tcccaggtat gggatgtgct cgctgccggg
     4681 caatgcgatg tactcggcgc cagagatccg ctcggccatg tatcggttcg cctcagggtc
     4741 gtcctcgatc cgatggagga tcaaggtcgg cacgtggatc gcgggaagga cgtggcggac
     4801 gtccgtgtcg tactccatga gttccgctgt aacggccgca cccgggctca tgctgcgccg
     4861 ggtgtaggtg gcgaaccatc ggacgagcgg ggcgtcggcg gcgatggatg ggaactcgcg
     4921 gcggatgaag tcctgcgtgc cccacatctc ctcgatgttc cggagccgct cgctgaactc
     4981 gtcggcacgc cacccccagg gtgcgtcggg ttcccacctc gccgacgggt acgagccatg
     5041 gatcacgagc gcgagagtgc gttccgggta ggtcgccgcg aacaggaggt cgagcatccc
     5101 ggactcagaa gcgccgtaga tcacggctcg cctggagtcg acctcgtcca taaccgcgcg
     5161 gatgtcgtcc atccgggttt cgaggtccgg gaattcgtcc gccgggaccg ggtccgacat
     5221 ccccaagcca cgcttgtcga acaagatcag ccgcgagaaa gatgcgagcc ttcggaggta
     5281 ggacgcgtac cgagggagct cccatcggta ctcgatgtgc gtggcccagg gtccgacgta
     5341 gacgaagtca actggcccgt tgccgacgat ctggtatgcg atatggacgc cgtccgctgt
     5401 cttcgcatag cgcgtttccg ggatctccac gatcacatcc tgggcctcca cgttcgcgcg
     5461 aggcaagcca ccggttgggc gtagcagcgc gctctcataa ccaccgtggt ccagcgagag
     5521 ggagcacggc gaggcccggg cgagcaacgc acctgactgg tgagatcgtg cgagaggggg
     5581 gattcgaacc cccacgccct tccgggcacc ggatcctaag tccgacgcgt ctgccaggtt
     5641 ccgccactct cgcgcgggcc aagcgtagac ccgtccgagg gcgctcactc ggcgggtagg
     5701 tcggcgggct catcgaccgc agggtcggct cctccatcaa cgggccggag ccccagaccc
     5761 aaacgacgcc gagccggccg gtgacggcga ccccgatcat gatcaccagc ccggcccgat
     5821 cgcccgtggg cacgccggcg agccgaagcg ccgagcgccg ttcttcgcga gctcgaaggg
     5881 cgaatcacgg atgatcacgc ggatcgctcg gccggacgac ggatgatggc cgcgaggact
     5941 ccagacgccg cgatgacgat cgcggtgatc atgcctcctc cgccaccggt cctcaccccg
     6001 agccgctcaa ccaggaggcc gatgaagaca agggcggcga ggcccgtcgc cggcgccagt
     6061 gccatccgca gcccgaaggg cccgccgccg aagcgggctg accagccggc gccggggagc
     6121 gcgatcaccg cgagcgagga cacccaccag gcgaacagag ccgacgagga cggccgttcg
     6181 gggacgaccg gagggtcgat cgggggcggt ggacccgcga cgacggccat ccatccgttc
     6241 gtccgccatt ccgggtgagc atcgatggct tcggcgaagc cgttgagatg cgatcggagg
     6301 accacgatcg tcggatcgtc cgccagcaga acccgagcgg ccagccaggt ctctcgggag
     6361 acctcgtcga agccgacgat ctcgggtcga agggtgggcc gcccctcggt gaggaactcg
     6421 gcgtcgccga ggtagaccgt cgtgcgcatt gcgagatggg cggggagctc tgcgcggatc
     6481 ctccgcatca ccggcacggt cccgaactcg ccttccgcgc ctttctcttg ccccgatccc
     6541 tcgtccacca cgacgatcgc gggacggccg gcctcgatca gatagctacc cagggcgcgg
     6601 aactccgcga gcgcctgatc ctggctccac ggcttcctcg tccgccacgt ctcccaggcg
     6661 aacgtgacgg agaagatcag cgccatgacg gccaagaggc caaccgcgcc ggcggccacg
     6721 ctcccgaacc gaccacgcgt ccacaggacc gcagcgacga ggccgagagc cccgagcacg
     6781 gggatcgcca atgagaacga cagggcacgt tgcagaggca ccgtccgacc ggcggcgaag
     6841 gcgagagcgg cggccgctgg gacgagcgcc cacgggacga ggagccatcc ggcccgccgc
     6901 cgggacggct cgcttccgtc gacggccagc catgtgccgc ccaccatggc ggccgcgacg
     6961 gcggcgggaa gccggtatcg agcgagctgg tccgagaggt gccggtccac cgacccgcgg
     7021 gacagcccga tgggagcatg cgggatgccc ggtgtcccga acaggagcgc ggcgatcccg
     7081 aggccggcgc cgcccacgac ggtcgtcccg actcgcccgg acgccgtctc gatcagcggg
     7141 agaccctgtc ggcgatcacg gagcgacgcc ggcagggacg cgagcgatac gacccccagg
     7201 agcaccgtga agaggagcgc gaactgccag tgcacgagcc acgccgcgac gagtgccgcg
     7261 gcgcccagcg cacgccccgg accgccgcct gcggcgcgaa gcacgcaggc cccggccatc
     7321 accagcagtg gctcgaccag gagctggtcc aggtagccgt tcgcggcgag cgcgacctgg
     7381 acggacgccc cggtcgacac gccggcgaga gccatccccc atccgggcac gccggggacg
     7441 gctcccgcga gcgcagcggc cgccaacgcg atcgcggccg cgaggaccgc cggaaggaca
     7501 taggcgagat ctcggacatc ggcaccggtg acggcggaga gggaagagaa gatcaagggc
     7561 aaccccggcc gatccgcatt ggtgttcagg gcttgggatc gtccatcgaa cgccggaaga
     7621 gcctcgaggc cgttctccgc caccacctcg gacctccaga cgtgctgcga cgtgtcgctg
     7681 ccggctggag cgacgcccgt cccatccacg acgtaccgcc cgaggaacag cagtgacggc
     7741 accagcacgc acgtcgccaa cccgaccgcc agcgtgagcc gcctcacccc tcctcctcga
     7801 gcaacgtcgg gagcacgccc tcggggacgg atcgccacga cccgggcaag tggttcgcgg
     7861 cgtagacgca gggggagctc gcggcggcgt ccccccggcg acgttcgccc atccgcggac
     7921 tataccgacg gcatcggagc gcaacgggcg aggagatgga cggtcgggac ctcgccggac
     7981 atcatgaagc cgatgctccg ccgcctcctc ctcctggtga gcgcggtact cctcgccgcg
     8041 tgcgcgacga gcgctctggc acccgtgagc cagccgtcgc ttcctcgctc ccggcctcgg
     8101 cctcccgccg acgtcggagt ggtcaagatg ctcgcgcgcg gagggacgct ggcagtcgcc
     8161 tctcggaacg acgtgctcgc cgtgcacgcg gcaccgggga cggagcgccc gttccaacac
     8221 ctcgcggccg acaacccgtg gggacagccg ctccgactcc tcgtcgtccg cgacacgatc
     8281 gaagagagtg agatctggtt gaggatccag ctgccgatct ggcccaacgg acaggaggga
     8341 tgggtgaagg cgtccgacgt ccgtctcgcc gaggcgacgg agcgcgtggt ggtggatctc
     8401 tcggaacggc ggctcgtgcg gctccgcgag ggaaggcggg tggcgaggct gcgtgtggcg
     8461 atcgggaagg cggcgacccc caccccacca gggcggtact tcgtctgggc gaagatcgga
     8521 accggacgcc cctcgggccc gtacggcagc tacatcctcg gtctctccgg cttctcggaa
     8581 gcgatcgagc cgtggaccga gtggcccggg cagccacggc tcgcgatcca cgggacccac
     8641 gatccggccg acgccggtca cgccgtcagc aacggttgca tccgcgtcct gaaccggctc
     8701 ctcggcgtcc tgcacgacgt cccgatgggc acgcccgtcc tcatccgacc ctaggccgag
     8761 gacccaggct gacctggtct tcgagcgccg tgcacgcgcg aaggcccgga tatcgacgcc
     8821 cgatggggcg ttatcgttcg cccatgatgc gctgcggtgc gtgcggggcg cacaacccgg
     8881 atggggcgag gttctgcaac gcctgcggag agcgactcgg ggtatccggg gccggtccca
     8941 tcgctgccgg atccggggaa cggcgtgtcg tcacgatgtt gttctgcgac gtgcgcggtt
     9001 cgaccgtgat ggccgaggag ctcgatcccg aggactggac cgacgtcatg aacgaggcgt
     9061 tcgcgtacct catcgagccg gtcgttcgaa acgaggggac ggttgcccgt ctcatgggcg
     9121 acgcgatcct cgcgttcttc ggcgcgccga ccgcccacga ggacgacccg cagcgcgcgg
     9181 tgatggcggg gctcgagatc gtctcctcga tcgcggctct ccgcgagaag gtcgctcggg
     9241 agcgagggct cgacctgaac gtccgcgtcg gcatcaacac cggcgccgtc gtggtcggtg
     9301 acgtggggtc ggagctcaag caggagtact cggcgatggg tgacgccgtg aacgtcgccg
     9361 cccgcatgga gcagaccgcg gagcccggct ccgtccagat caccgaggag acctaccggc
     9421 tcgtcgccga cctcttcgag gtcgagccac tgggcggtgt ggagctcaaa gggaagcgac
     9481 gccccgtcga ctcctaccgg gtgctcggtc gcctggacgc tccatggacc acccgggcgg
     9541 cccggcgcat ggacgccccg ctcgtcggac gcgaagagga gatggcggcc atacgcgcgg
     9601 cgttcgaccg ggcgcacgaa gggcgggggt cggtcctgct cctcgtcggc gatcccggga
     9661 tcggcaagag ccggctgatc gaggaggcca acgcgatctg ggcgatagcg gaaccggagg
     9721 acgatcggcg ctgggacttc tggggatgcg tgcccttcga caccatgcag ccctacgcgc
     9781 aataccgacg actgatgaag gagcgggcga acgtcaagga gtcggatccc gccgagaccg
     9841 tgcgggccaa gatcggcgat ctgatggagt cgatcgcgct ggagggatgg gaagagcgga
     9901 gcgaacgggt cgcgcgggcg ctcttgggcg tcgagcgaga ggacgaaccc catctggagg
     9961 gcgaggagtt ccagggcgcg gccaccgagc tcgtcgtggg ctcgaccctg gcacagggcg
    10021 gtcgccggct gatcgtcttc gaggacctcc actggtgcga ccatgcctcg ctcgagttgg
    10081 tccgagcgac caccgagctc gtcgcggaga acccgatcgt ggtcctcgcc tcgttccgcc
    10141 cggatcgcga ggcgatctcg tgggagttca aggagtgggt ggagaccgat ctcgcggcgc
    10201 atgcgacggt gctcgagctg gactccctct cggcggagca gagcgacgag ctgatcgggg
    10261 ccttcttgcc gatccctgag atgacgggag ccgaacgaca gcggatcctc cgcaacacgg
    10321 agggcaatcc gctcttcatg caggaggtgg ccagggcgct gatcgacggc ggcgtcgtcg
    10381 agcgcgggga tgacgggtgg cggctgacgg ccgacgcgac agagatcgtg atccccgaca
    10441 cgatccagag tctgatcacg gtcgggctcg accgcctgcc ggagtcgacg cgtcggacgg
    10501 tgcagacggc cgccgtgatc gggaggacct tccaagagga gctcctcgtc gcggtcgccg
    10561 gccgggccga cgtccgggac gatctccgag agctcgtgcg ccgcgacctg atccgggtca
    10621 ccggcgacgg ctcgccggcc gagttcacgt tccgccatgc gctgacgcag gaggccgcgt
    10681 acggcacgct cctcgccaag caccgccgcg ccacccaccg gcgagtcgcg gaggttctgg
    10741 aggagatggc gagggagcgc ctcgacgagg tggcgtcaca gctcgtgcgc cacttctcgg
    10801 aggccggcga ggacgactcg accgtcacgt acgcggcgaa ggccggggat gcggcggccc
    10861 ggctgtacgc gaacgccgag gcggaggcgc actaccgcac ggggttggac gccgcccgtc
    10921 ggatcgccgc gccgtcgccc ctcctgcgat cgatgtacga acggcgaggc agcgccctcg
    10981 agctgatcgg ccgctacgac gacgcgatcg agaactacga ggagatgcgc gcggaggccg
    11041 tggcgagggg cgacgacgcg atggagctgt cggcgaactc gagcatcgcc ctgctgttcg
    11101 cgacggccac cccgaagttc gacccggagc tggggcggcg gctgtcggag gagctcgtgg
    11161 tcatggcgcg ccggatcggc gaccgctcct ccgaggcgag ggcgttgtgg aacatcgtcg
    11221 tggcgaacgt ctacggcgga ggggacgccg cccgagccgt cgaggccggg gaggcgtcgc
    11281 tcgtgatcgc tcgcgaactc ggcgagcgcg agcaactcgc gttcaccttg aacgacgtca
    11341 ctcgggccta cctttcgctc ggcgacttct cgacggccgc gcaccggcac gccgaggcgc
    11401 ggcggctctg ggaggagctc gacaaccgtc cgatgctggg cgagaacctg accgtcggca
    11461 gctcgatgca cctgttccgc ggcgactacg cggccgcgct cgcggactcc cacgaggcga
    11521 tcaccgtttc tgagtcgatc ggcaacgcgt ggggtcagtc gcacgccctg atcacggtct
    11581 atcgcgtcga gctcgagcgc ggtgagctgg gtgccgcgat cgcctcgatc cagcgctgcc
    11641 tcgacctcgg ggagcagggt gggttctcct acgccgcgat cgccacgcga gcggacctct
    11701 cccgggtcat gacctatctc ggggacggcg agcgggggct cgccctcgcg gaggagtcgc
    11761 tcgcgatcgc gcgcgaccgg gtgccccccg ccctgtcgat cgcccgcgtc tcggaggccc
    11821 tcgcgctcat ctcgctcggg aggcgtgacg aggcgcgcgc gccgctggac gaggtggacc
    11881 tgatgatgct gcccgagccc gaccggacgt tcctccgggt ggcttccggc gcggcgcggg
    11941 ctcgcctcgc gctcctcgac ggagaggtcg aagaggccga ggccgtcgcc cggtcggtca
    12001 tcgaggagct ccgcgcgagc gaggtggaga tcctcgtggc gggggcgatg gtggtgctcg
    12061 cgagggcgct cctcgccgcc gatcgactcg acgaggccgg ccacgagctg gccgacgcga
    12121 tcgcgcgtgc gaaggggctg ggggagcgcc gggtgctctg ggaggcactc gcgttgtccg
    12181 ccgatctgca cgctcggcgc ggtgcccagc gggaggcggc cgatctccgc aggcgagcgc
    12241 gcgcgatcgt cgacgggatc gcggccgggc tccctgacgc agacctcagg cgccgattcc
    12301 tcgttcgcga cgacgtccgc gccctggaag gaggctgaca tgaccgagcc ggtagtcgac
    12361 aagccggagt tcccgcgcga gtacgggaac cctactcaac gcctggactg ggcggaggtg
    12421 gagcgcaagc tcgagagcgc cagcgtgtac tggatggcga gcaccagacc cgatggacgg
    12481 ccgcacgtcg tcccgcgcga tgggacctgg ctcgatggcg gactctatta cggagggagc
    12541 ccggagacgg tccacgcccg caacatcgag aacaacccgt ccgtcgcggt gcatatcggc
    12601 gacggccagg aggcgatcat cgtcgagggt gaggtcgtga tcgagaagcc gacgcaggag
    12661 atggccgagc gactgtcaga tgcgtcgtcc gccaagtacc cgcagtacgg gcagatggac
    12721 ccgagcatct acatgggagg cgtgtcggtg ctccggccgc gccgcgtgct cgcctggacg
    12781 agcttcacgg agaacgccac gcgcttccgg ttcgcgtagc gcttggtggg cccccgggga
    12841 ctcgaacccc gaacctgcgg attaagagtc cgttgctctg ccagattgag ctagaggccc
    12901 ggcgcagtgt agcaaccggg cacgcgagaa cgagcctttc ggcatgtttg cggggccgtc
    12961 gatatgggga ttcctccgcc gatggaacgc tgatgccgcc acggaggcgg caccgagtga
    13021 aggaggcgtt caccatgagc atgacgccgg agcaccacca ccacgaagat gaggtgacac
    13081 ccgtccgtga cccggagggc agcggtggcc tcgttggcta cgcggcgatc aagtacatcg
    13141 cgatcgtcgc gatcgtgctg gcgatccttg tcttcctcgc tctgtacctg atcccggccc
    13201 tgacctagga ggagaaccga tgcttgcgga cacgatagcc atcctggccc aggccaagga
    13261 gcagacgacc gataccatcc tttggatcat cgcagcggtg ctcgtgatcg ccgggatctt
    13321 cgcgatcgtc cgcagtcagg tgatctgggg catcgtcctg atcgtcctcg gactgctggt
    13381 cggaccgggc ggcgtgagcc tgctggactg accgaacgct cgagaaggga acgacggtgg
    13441 ccgcggcccg aagggccgcg gccacctcac gtctggggtg accgagggga cttgaacccc
    13501 cagcctccag gaccacaacc tggcgctcta accagttgag ctacggccac cacgtgcgag
    13561 ccccaagcgt accggaagcg acctcctggg ttgcctcggg aacgctcgtc gggcatcatt
    13621 gaacggccgt tcaacttttc ggaaggtggg tccgatggcc gtcaaggaaa cgatcgactt
    13681 cgacctcgat ctgatcgatc gcctcatcgc cgacgaggag gcgacgctcg agccgaagca
    13741 tcgcgcatcg atcgagtacc gcagggtcgc cgagcgaacc gtggccgggg gcgtcgcatc
    13801 ctcctggcag gcatcccccc cgcacgcgat cttcatcgac cgcggggagg ggagccacat
    13861 ctgggacctc gacggcaacg agtacgccga ctaccacctc ggctacggcg cgatggtgat
    13921 cgggcacgcc cacccgaagg tcgtggaggc gatcgagaag cgcgcgcgtc tcggcaccca
    13981 cttcgcacag ccgaccgcgc agctccacga gatcggggag aacgtcgccg agcgcttcgg
    14041 gctcccgctc tggcggttct gcaactccgg gaccgaggcg acgctcgagg ccgtccgcct
    14101 gatgcgcgcg aacacgggcc gggacctgat cgtgaagatc gagggcacgt accacggcca
    14161 ccacgattca ctgatgttct ccgtggcacc cgatccggag ctgatcggcg cccgggagca
    14221 tccggtcacg gtcccgcagg cgatggggat cccgcaggcg ttcgcggagc tcgtccgcgt
    14281 ggtgccgttc aacgacctcg ccgaagcgga gcgcgcgttc accgagaacg agggacgcgt
    14341 cgccggcatg atcgtcgagc ccgccatgat gaactgcggc gtcgtgttgc cgcagcccgg
    14401 ctacctccag ggattgctcg acctctgcca ccgccacggg gcgtatctcg cgttcgacga
    14461 ggtgaagacc ggcggcacga tcgcgtgggg aggcgccgtc gaggcgttcg gcgtccggcc
    14521 cgacatcgtc acgctcgcga aagccttcgg cggaggcgtc ccgtgcggtg cgatcggcgc
    14581 gaccgaggag cttttcggcc ccgtcctccg cggcgagcac gacatcgccg gaacgttcaa
    14641 cgggaacccg ctcacgatgg ccgccgcgcg ggcgacgctg ctcgaggtgc tcacgccgaa
    14701 tgcctacgag cgtctccacg cgctcgacgg cgccctgaag gacggcatcc gaccgatcat
    14761 cgatcgccac cggctgcccg cgtacgtcac gggtatcggc gcgaagggat cggtcaccta
    14821 ctcggggcgg gaggtgcgtg agtaccgcga cacgatcggg atcgacgagc gcatcacgta
    14881 tctggcctgg ctgatgcagc agaaccgagg cgtgttcaag tcgccgtggg ccaagatgga
    14941 gacgtggacg ctctcgatcg tgcactcgga cgacgacgtc cgccgctacg tggagaactt
    15001 cgaggccttc gcggcggctg tgtcaggaac ctgaggcgga gccgctgtag cggggtcggc
    15061 gccccgtctc acggacagcg gctatcccga cgatcgagag cacgagcgcg gcgacgatcc
    15121 aggggagcgc cccccaaccg gtaaaggcca gcagcttccg gatgatctcg gcgacccttc
    15181 ccgccaggga atcctgggcg gtctggctcg tcgtctcgca cgtcgtcggt gtgaccgtga
    15241 cgcagggtgt cgtgccgatg tcggaggccg gaacagcccc ttctcgtgcc tccaataggg
    15301 ccgctccgcc ggaggggccg gactccatcg ccctggtcga tgacgcctcg ccggtcccat
    15361 cactgacgga ggatccggct gaggacgcac ccggctcccc gcccacagac gctcctgtcg
    15421 gcaaggatgg atcgctcaag cctgagtcgg tcgtcccgat ggcgcccgtg acgccgccga
    15481 cgacgtccgt ggtcgagccc gcgaccgcat ccacggtccc ggatgtggcg tcggacacgg
    15541 ggtccacggt cccggaggtg gtacctgaga cgccatccac cgtgtcgctc gtggtgtcca
    15601 cgacctcgtc cacggggtcg ggcagtgggt cggaaagggt atcggccgtg ctggaaacgg
    15661 tttcggcagc ttgatcgacg gtcccctgga ccgggtccac gagatcggcc gcgccggagg
    15721 tggtctcggc cacagatccg agcagcggat cgatgggtcc atccgctgcg agcgcctgcg
    15781 ccggtgccca tcccagagcc gcgaccgtca tcgcaacgga gatcagcgtt ctacagcggg
    15841 tgcctgccga agcgctcctc atcccgtccc tccagaccgt tctgccgttc cgatgcctcc
    15901 gccataccct gtccccgtcg agcgtaacca gacgcccgta ggtcgttcgg cgaggcgagg
    15961 aacgacggcc gtcccgtgac cgggcgccca tcacgctctc aaaccagccg gaacgacccg
    16021 atcacctgta cgaccgcatc caggtcgtcg ggatctggat cggtcgggaa ccgaaccacg
    16081 atcgcgcggt tcccgtctgg gcccccgatg gtgatgacga cgaagcggat ggatgtcccc
    16141 accgcatctc gcgccgtccc gcccacggac ctcgagggat atccttgctc ggttcgtgct
    16201 gtctccgtcg cgagggtctc agcgccagtg aacgttgcgg tgatgcgttg gaggagctga
    16261 tccgacgccg gcgccaggcg accgggtggg gctcgcagga atgtcaccac cacctgatcg
    16321 gccgtgtcgg agagcaccgt ggcctgggag gaagcctcga tggcccagtc actcgggtac
    16381 gagaatcggt acccgaggcc atcgttcgtg tagtcctccg aggtgatcgc ctccgaggtg
    16441 ggagccgggg cttgctgcgt ggagccgatg tccaactctg tccgggaggg gaggacgatc
    16501 ggcgaggggc ccgagtcgat cctcgccgcg ttccagaggg gaagcatcac gaccatcgcg
    16561 aggaccaggg agatggagac gcctgccgct gcgaccgcac ctcctcgaga cgccgggccg
    16621 ggcaagcgtt cccaacgcgc ggagagatgg aggaggaacc ggttccagcg gatcaggagg
    16681 agttcgtcga ggagctccag acgctccgcg ccagacagca gccggccccg gatgcgggtc
    16741 cacaacccac ctcgatggcc gttcttgaac aggtgttccc gacggtcggc gagccgatcc
    16801 cgacgcactt ccaggcggtc ggtgagccgg tagacccgtc gctcgcgcca tcgctcgaac
    16861 ctcgcggagc gccgctcctt gcgggaggac gtgcgcgatc tcgcccgccg tccaacgggg
    16921 gcagcaggag acaacgcgag aagttctttc gacatgtcca cgttcggggt ctatccaccc
    16981 cctccctggt ccaaacgcga cctcggatcg cggtagatct cgagttgcca agaagagcat
    17041 tccacagatg ggctagggag gaccacccac acggtcctcg gtcactcggg ggatccaacc
    17101 tggccgcacc cagccgtgat ccccatgcgg gcggctacca tcggagcgtg acccgatcga
    17161 gcggcgccga agtccggaag aaggccgagc acgcagacgc ccggctcagg ccgttcctgg
    17221 cgcgggtggg catccccctg ctccggatct ccgtcggcgt ggtgttcctc tggttcggtc
    17281 tgctcaagat cttcgacgtc tcgccggtgt ccggactcgt cgcgaagacg atctactggt
    17341 tcgaccccga cgtcgtcgtc ccggcgctcg gcgcggtgga ggtcttcgtc ggcctctgcc
    17401 tgctggtggg caggttgatg cgactcgcgc tcccgctcct cgtcctccag atggccggca
    17461 cgttcctggt gctcgtcctc ctgccggaga tcgcgttccg ggacgggaac ccgttgctgc
    17521 tcaccgtgga gggcgagttc gtggtgaaga acctcgtgct gttctcggcg gccctcgtga
    17581 tcggctcccg gctcgcgccg ctgggcgagg gcgggaccgg cagcgcctga cccggcggac
    17641 ggcgtagtct cctcggccat ggataccgct cgaaccgtgg acatcgatct ggattgggtc
    17701 gaggagctga cgaagcgcga gaccgaagcg ctcgaccgca gacacgctcg ctcgctctcg
    17761 taccgtgagg aggccaagcg ctacctcccc ggcggcgtct cctcctcgtg gcagtcgtgg
    17821 ccgccacacc cgatctacgt gcgacgtggc gagggcgcac acgtgtggga catcgacggg
    17881 aacgagtacg tcgacctcca caacggctac ggcgtgatgg tgatggggca cgcgcatccg
    17941 aaggtggtgg aggcggtgca gcgccgggtc gccgagggca cgcacttcgc gcagccgacg
    18001 gaagaggcgc tcccggtcgc ggagaacctg gcggagcgct accgactccc acagtggcgc
    18061 ttcggcaact ccgggaccga ggcgacgctg gacgcgtgcc ggatctcccg cgccctcacc
    18121 ggccgctcga agatcctgaa gatcgagggc acgtaccacg gtcatcatga ctcgctgatg
    18181 gtcagcgtgt tcccgtcgga ggagcaggcg ggctcgcgcg atcgtcccgt cgcggtaccg
    18241 cagacgaagg ggctgccgaa ggagttcgtc gacctcgtcg tgaacgtccc gttcaacgac
    18301 ctcgtggcgg cgaagggcgc cttcgccgag caccccggcg agttcgcgtg catgatcgtc
    18361 gagccggcga tgacgaactg cgggctcgtc ctgcccgatg ccggctacct cgccggcctg
    18421 aaggagctct gccgggggga cggtgcactg ttcgtgttcg atgaggtgaa gaccggcttc
    18481 acgctcgcgt ggggcggtgc cgtcgaggcg ttcggcgtcg tgccggacct cgtctgtctg
    18541 gcgaaggcgc tcggcgcggg cctcccatgt ggcgcgatcg gcgggaccga cgaggcgatg
    18601 ggcatggtga tccgcggcga gctcgaccag cagggcacgt tcaacggcaa tccgctcacg
    18661 atggccgcgg cgaaggccaa cctgctcgag gtgatgacgc ccgaggcgta tgggcgcttc
    18721 gacgagctga actcggtcct cggccgcagc ctggaggcgt gcacgaagca tggcctgccg
    18781 gccaacgtga aggtgttggg ggccaagggt tccgtggagt tccgtgatca gccgatccac
    18841 gagtaccgcg atctgtggga catcgatgac cgcgtgccgc agctcgcctg gctgtggcag
    18901 ctgaacagag gggtgttcaa gtcacccggg tcgaagtggg agagctggac cacgtcgatc
    18961 gtgcactccg acgccgacgt ccagcgctat gtggacaacc tcgaggagtt cgccgcgacc
    19021 gtcaccacgg gcacatagcc ttgcgtcggc gagtcgtgcc tcacggctag actcgccgcc
    19081 atggacgagc cgcaggtcgg cgtgatcatg ggctcccgct ccgattggga gacgatgaag
    19141 cactgctgcg acacgctcga ggacctcggc atccggttcg agagcaaggt ggtctcggcc
    19201 catcgcacgc cggagctgat ggtcgagtac gcgaagaccg ccgccgaccg ggggctccgc
    19261 gtgatcgtcg ccggcgcggg cggggcggcg cacctccccg ggatgacggc cgccaacacg
    19321 cacctgccgg tcctcggggt gccgatcgag tcgaaggcac tgaaagggat ggactcgctc
    19381 ctctcgatcg tgcagatgcc ggccggcgtg ccggtcggat cgctcgcgat cggggtgagc
    19441 ggcgccgtga acgccggcct cctcgcggcc gagatcgtgg ccctcgagga cgagcacgtc
    19501 cgcgagcagc tcctgaagtt ccgggagacg cagacccaga acgtggtcgc gcatcccgat
    19561 cccgacgaac ggtgacgcgc gtcgggatcg tcggcggcgg ccagctcggc atgatgctgg
    19621 ccgaggccgg agcccggatc gggatcggga tcgtaacgct cgaccccgct gcggacgcgc
    19681 ccgcgacccg ggtcgctccc tcgatcgtgg ccgcatacga cgacgaggcg gccctcgccg
    19741 agctggcctc ggtctccgac gtcgtcacct acgagttcga gaacgtcccg gtggcgtccg
    19801 ctcgtttcct cgccgaacgg gtcgcggtct cccccccgct cgaatccctc gagttcgcgc
    19861 aggaccgcct cctcgagaag accctgttcg aagaggtggg gctggcggtg gcacctttcg
    19921 cgccggtctc ctcgaccgag gacctggccg aagcgatgga cctcgtcggg ttcccgagca
    19981 tcttgaagac gcgtcgtctc gggtacgacg gcaaggggca ggctcggctt gagcgcccgg
    20041 accagggagg cgacgcctgg cgggaggtgg ggcaggcccc ctcgatcctg gaagcgctcg
    20101 tgcctttcga tcgggagctg tcgatcctcg gggcgcgtgg tcgcgacggg gaggtcgtgt
    20161 tctatccgct ggtcgagaac gtccaccgtg acggcatcct ccgcacgtcg acgcccgcga
    20221 cgtcggtcgg ggtcgcgttg gcgtcgaagg cggaggatct cgccttcgcg gtcatggagc
    20281 ggctccggca cgtcggcgtg ctcgcgatcg agctcttcga gacgggggag gggctggtgg
    20341 gcaacgagat ggccccccgt gtgcacaact ccggccactg gacgatcgaa ggggcggcga
    20401 cctcgcagtt cgagaaccac ctccgagcgg tctccggcat cgagctcggt ccgacgaccc
    20461 gaacggccta cagcgcgatg gtgaacctga tcggttcgga gcctccggac gaggcgttcc
    20521 ccgcagatcc gggcgtgcac gtccatcgtt acggcaagca cgcccgtccg ggtcggaagc
    20581 tcggtcacgt gacgatcgtg gaggacgacg cagataccct catccaggta ctaagggagg
    20641 tcacccgaac ggccgacaac agctgagatg gcctacctca cctgccccga ctgccggatg
    20701 ccctccccag tggaggacag cgccatctcc taccgctgct tctcctgcgc cgcggagatc
    20761 atcttctgcg cgtgccaccg gtgcgagtac gaccaggcga tcccctcgcg ctggttcggc
    20821 gccttcacgt gtggccgctg cgactcgaag gtcgacatcc ctcggcagcg cttctacagc
    20881 acgtcggtga aggcctcacg tgtcaagggc tacgggtaca cgtacccgcg cttctagcct
    20941 gcgcggggtt cctgttcgct tccctcaggc ggtcgctccc atctgctcag ccatcgcttc
    21001 gatgttgcgc accgcctcgg ccgaggcggt gtcgttcgct tccgccgagg ccaggatctc
    21061 gctccagagg atgttccgat cgtctcccat ccgatcttcc ggcttcccgc atccacagtt
    21121 ggcacacatc ggtctctcct ctcgctcgag catgcgttcc gatacaccta tgccctggga
    21181 tgatcgatcg gaaaccgccc cgcggggggg cccaccccgc gtctactccg actctgtgga
    21241 tggagcggca ctcgctgctt cggacttggg gcacttctgg tgcttcgctc gctggtccgt
    21301 gctcgaccag aggatgcgct cgcccgcgtg gatatcgccg ccgcacctga ggcatttcgt
    21361 cgcgtagctc gcggttcgct ggaacactgc caccggatct gctcccttct gctcggccgg
    21421 tccgtccggc cggcgcgcca actgtgaagt ctcaccaccc gtgtcccgga atggctccgg
    21481 cacctatgcg acatctcggg ccaccttcct cggcctgcct ccaggattgg cgagcgcccc
    21541 gaactccttg agcttgtcat ggcgggctcg gtgagtg
//
LOCUS       DASUYD010000108        14249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1158535, whole genome shotgun sequence.
ACCESSION   DASUYD010000108 DASUYD010000000
VERSION     DASUYD010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14249
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158535"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF195_05840"
     CDS             <1..283
                     /locus_tag="VF195_05840"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HEX6580373.1"
                     /translation="WALGLYDLLTPGDLTRGRTIAGTGAIDLEGNIGPIGGITDKVGA
                     AQSVDADILLVPQDNFGELRDVDTGDLELIPVSTLEEAVEALDPSETTA"
     gene            299..3157
                     /locus_tag="VF195_05845"
     CDS             299..3157
                     /locus_tag="VF195_05845"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEX6580374.1"
                     /translation="MSTTTVRIPYPRRRWPVVAVGVLVVGFLILTFLSNFYIDVLWYR
                     EVDLSQVFWTRIWAQAGLASASFVTFFVLLLANLIVTRRLAPTIVALTPEQEIVERFK
                     ENIEPYLRWAVPVGAAILSIFVGLAAAGHWQEFLLWRSSSGIEFGNPDQVFGRDPAFY
                     VFTLPWLKYLQSWLFASLVGITVIVGIGHFLQGGIRPQAVGLRDKVDPQVRAHLSVLL
                     GLILLAKGWGYYLGRFDLLTSERGVVQGASYTDVKAQLPALTFLAIVAVLCAVMFFVN
                     ARYRVWSLPIIAVGLLLIVSILLGTAYPAFIQQFRVRPNEQQYERPYIGRNIAGTQRA
                     FGLTDIRLEERPFDPVVTADDVEANAATIANIRLWRPSVLKENFESVQRIRQYYDFND
                     VDVDRYEIDGERRVLMVSGREVTQSGISQQARTWQNEHLVYTHGFGTVAAQVNSTTTD
                     GGPLLTLEDIPPDGEPSTDQPRIYYGEIDDVDFVVVNSATEELDFEGASGETPFTYDG
                     EGGIDLGGYLRRALFAWRFQDANLLISGQVDSESRVLFRRDIEDRAKATLPFLTFDHD
                     PYLAVTQEGYVWIMDAYTTSNAYPYSQSVDMSEVTANLLPRQDVNYIRNSVKAVVDAY
                     DGSVTYYADLDEPIMQAWSRAFPGVFTPIDEAPEEIAAHFRYPENLFQAQAFQYANYH
                     VEEPDAFYRKQDFWEVPGDPTLQSDVIGPDGSPSPAPNASARKLLPNYQLLRLPGDTE
                     ERFHLVIPFQPENRLNMVGWMAANSDQDGYGELVAFTLPSGRDVDGPGLVFARVNSDQ
                     EFSEARTLLGTGGSRVLFGDLLTIPIEESILYVLPVYVRAAGTSAVPELELVLVVNGS
                     TVTLGRSLTEAIQESTGAVTGEEPPPPDGGEGTVEQQIERLLTQAVTHFAAAEEALRA
                     GDLATYQSEQEEARALVEQANELLAAGTGGEPSPSPSP"
     gene            complement(3154..4719)
                     /locus_tag="VF195_05850"
     CDS             complement(3154..4719)
                     /locus_tag="VF195_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6580375.1"
                     /translation="MTAARTVTSLLDGSAGGDSPGGTLTIVNPARLEETVVEAQLGDA
                     STFLDACRRAKAAQPTWAGVPAPVRGRAIQQLGRLVEDNFESLSRLITREIGKPIRES
                     RGEVQEVIDTCTFFLGEGRRLYGQTVPSEMPDKQLFTFRVPVGVAGIVTAGNFPVAVP
                     SWYIVPALLCGNAVVWKPAEYTPATAEAFAQLILHAGFPRGAFQLVLADGPQTFEGIE
                     RALGDGVLDKVGFTGSTEVGSRIGELCGRHLQSPCLELGGKNPLVVMPDADLDLAVEG
                     ALFSGFGTAGQRCTSLGTAIVHRDVHDDFMARFTKAATDAPIGDPTEDVLYGPMISAR
                     FSERYERWLELIRPHHTASGSDSIGRITADKPRTGFVGDPETGLYYHPVIVDGVTIED
                     DLYRTETFGPIVGVAAFDTFDHAMDLANGHGYGLSSSIYTNDPLHVFRFRDRISAGMV
                     SVNNSTSGAEAHLPFGGNGRSGNGSRQSGPWVLDQFTRWQAMNWDYSGHLQKAQMDTA
                     ELDADLGFRLPAD"
     gene            4808..5128
                     /gene="fdxA"
                     /locus_tag="VF195_05855"
     CDS             4808..5128
                     /gene="fdxA"
                     /locus_tag="VF195_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6580376.1"
                     /translation="MAYVIAEPCIDVKDKACVDECPVDCIYEGPRMLYIHPDECVDCD
                     ACVPVCPVTAIFPEDDLPSEWKQFTAVNAEWFETTGLGSPGGAAQAGPQPKDHAIVEG
                     WEAV"
     gene            complement(5125..6228)
                     /locus_tag="VF195_05860"
     CDS             complement(5125..6228)
                     /locus_tag="VF195_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580377.1"
                     /translation="MDELVFGLREAAWPLIRRELSLSYLQIGLLLSIPGIAGTLVEPG
                     LALLSDIGWRRRVVLGGGVAFVLALIGTAAASGFGLLLAAACLLFPASGAFVSLAQAT
                     WMDLEPASTERNMARWVVAGSVGGILGPLLLVGVVGLGSGWRGTTVVAALMTVPVLLS
                     ARRLRFPAPHPDTAHLRATVRGAIAALRERRVLRWLTLVQLTDLLGDVFVGFSALYLV
                     DEGGASPSVAAAGVGILSVASLLGDALVIRILRRMDGLSWLRWSAMGALVVYPAFLVV
                     GSPAGKLILLLPLGLVRSGWYAIVQARLYSELPARGGTAIAVGAPADLVGVLLPLAIA
                     SVAERWGLHSAMWLLLGAPIALLTLLPRAREEP"
     gene            6357..7820
                     /locus_tag="VF195_05865"
     CDS             6357..7820
                     /locus_tag="VF195_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580378.1"
                     /translation="MDRPKDDQIALARRLLLFVGLAFLLAQLLAFSLHRPPSWDEAIY
                     LSQVAPGAEALPFVPSRARGITFLAAPVLQLGGSLVHLRLFLVVASAAAMTAAFRSWA
                     KVIGPGAVAAAVVFAGAWPALFYGSELMPNLWVALIGVAATAILARRLATGEGRYDEL
                     LAGGLVAVAALLRPLDAVVLAAALILLPIAVRRATIAWTVHVVLGLAAGWAPWLVEMI
                     GRFGSPAEAFTAAARLGHTGRWALLENVHQYLALSDGPSIGPIAEPHIPVSGILWLLG
                     MTALVVAGIRAAAGARLLPSLVVPTATGAALAAEYIVFTDAQAPRFLLPALALLAIPA
                     GLGFVWIADRARDQRPARTGRWVAAAAASTLVVAWAFLQVGIASRLEAGVTQQRASAQ
                     RAGSRVRALAGPGPCHVYSEASFPIVGYAAGCLAAPIGNVVRAWPERAARLERDGVRP
                     FLVLHRAEQPAVPKGASPLAKIPSDGQSSSWFIYGTG"
     gene            complement(7805..8635)
                     /locus_tag="VF195_05870"
     CDS             complement(7805..8635)
                     /locus_tag="VF195_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580379.1"
                     /translation="MGEPVHPEPGAGRSRAERRRRRERAIARWRQWLSDRDAFLESSL
                     EPGERVIARSGAHPLVTDRRILTARQLRDPSRGRTWVLDPVAFDQIVGWSEGVHHDGR
                     PILRFTHHPLTRITWVPARRFLWWTWDDAVGPVEETTTTLSFGGRTNPVLRAIVAELD
                     RRTVPQGPSFVIRPAGTRSQRLKESRRPLEVQTRAGRISHVLRRATLSIYPGKLAWPI
                     RASSWIVVAALAWLASPWLVLPAIIASELVWIVVMRWSSPGRRTIRAADVDPADQPVP
                     "
     gene            8727..8800
                     /locus_tag="VF195_05875"
     tRNA            8727..8800
                     /locus_tag="VF195_05875"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8761..8763,aa:Met,seq:cat)
     gene            complement(8782..9621)
                     /locus_tag="VF195_05880"
     CDS             complement(8782..9621)
                     /locus_tag="VF195_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX6580380.1"
                     /translation="MNVATLAERPRVPRTEREAWTLAEVRAFVARADRDRAAIWRLAL
                     STGLRRGELIGLWWDDIDLEARSLKVRRQVLVRGRSSSSAPRLYIRATTKGRRPRTIR
                     FDGVTRDALKAWKAAQNEDRLAFGPAWHADGGLGLDAAWIGTEADGEVVHPDTLRDRF
                     VRLAKAAGVRELVLHGARHTFATLSLASGVRADLVSRALGHATTGFTLDVYSQPSGEE
                     ELAAADRIGEALGQRERVSRLTPENRESPGKSEAPEPSRAQGPRTVRPASGPSRLRRG
                     DRI"
     gene            10056..10550
                     /locus_tag="VF195_05885"
     CDS             10056..10550
                     /locus_tag="VF195_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017563594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580381.1"
                     /translation="MAEGTIIREFRKDDLGAIIELSLRAWEPVFASLRKVLGDDVFLR
                     LHPVWKEDQADEVRSACTNEQRDVFVAVTDGRPVGFVAVALNVFHNGMGAIDIIGVDP
                     DYQRRGISSRLTEFATEHMRSRGMDIAMVETGGDPGHQPARAAYEAAGFTLLPIARYF
                     RLLD"
     gene            10674..11240
                     /locus_tag="VF195_05890"
     CDS             10674..11240
                     /locus_tag="VF195_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007820204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580382.1"
                     /translation="MTETPTLHLTVGLPGAGKTTLARKIAHGDKAIRFSPDEWMIPLF
                     GTTWREPDAVGKRDALEGRLLWAAHEVLGVGGSVVLDFGCWTEAERWAIRAVAEHAGG
                     RFRLHFIDLPEAERRARATTRWQGDPGSTFEMSPRDHVRFLGLFQAPTEAEIAAEHLP
                     APPRGHRSWLAWAANRWPSLPDFADEQE"
     gene            11583..12230
                     /locus_tag="VF195_05895"
     CDS             11583..12230
                     /locus_tag="VF195_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580383.1"
                     /translation="MDSFAPVQLPDDIQRRLNVAKAAGALALIAVWGWFALVKGDQTP
                     VFVYLNIAVHEVGHVLFRPFGELTMLIMGSGFEVLFPFAVGAYFLLRKRDLVSTAVAW
                     GWAASAQASAATYIGDADDGRLALLGATGPDAAGDWERILGVEFFDKVYLADRIAGPV
                     RTVGFVLWGVALCLAVWAIVRNRQLVRAAGASPKPRVTAEPRAPLVPIHDEEMWR"
     gene            12298..12723
                     /locus_tag="VF195_05900"
     CDS             12298..12723
                     /locus_tag="VF195_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580384.1"
                     /translation="MAGSTVLIEWLRQVPLFSACSARELRAIAGVVKEVDHPRGTVIA
                     TEGDPGVGLFVIVDGEAEVTIGGKRMAILRRGDFFGEIALLDGGPRSATVAARTDVRL
                     LGLTEWVFRGLLQEHPSIAVKTLESMAGRLRDATQAATA"
     gene            12737..13513
                     /locus_tag="VF195_05905"
     CDS             12737..13513
                     /locus_tag="VF195_05905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6580385.1"
                     /translation="MPVLARPDGVRLYHELHGSELAPPLILLEGMGGDIPGWRRNIPV
                     LARELRVIAYDFRGNGNSDEPPGRVTMATFVEDTLALLDALGVGRAHVYGQSFGGMVA
                     QELALTHVERVRTLILGCTHAGPRHVLRGKRSHVPKGEPWRSLYAPGFPDLHPEHVAE
                     DLRVGAAQPKHPEGGRRQWEAMQGFDSLDRLRNLKVPTLVVHGSEDQAIAPENAELLA
                     ERIPGAELLLLEGAGHLYHSERAGKADAAVLDFIRRHDDA"
     gene            13506..>14249
                     /locus_tag="VF195_05910"
     CDS             13506..>14249
                     /locus_tag="VF195_05910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6580386.1"
                     /translation="MPDAPEEVVRLAEERADARSSRDFAAADALRDRIAGSGWTVVDD
                     PGGYRLEPISGSATELPARRRSAEVPSILQDAPDMDATVHWVCEGWHEDIERAIAAFR
                     AHEGGRSVQYVVADVTGRDPTTWGEGVDVVWLKQGTGWAAARNAGIRRTRGRIVLMAD
                     GSVEPTGDVFGPLEEALEDPGVGVCGPFGIVTHDLRQFDESRTGDVDAIEGYLMALRR
                     ETLVEAGFFDEKFRWYRTADIEFSFRVKDL"
BASE COUNT         2219 a         4873 c         4924 g         2233 t
ORIGIN      
        1 gtgggcgctc gggctgtacg acctcctcac ccccggggac ctcacgcgtg ggcggacgat
       61 cgccgggacg ggtgcgatcg acctcgaggg caacatcggg ccgatcggtg ggatcaccga
      121 caaggtgggc gccgcgcaga gcgtggacgc ggacatcctc ttggttcccc aggacaactt
      181 cggcgagctc cgggacgtcg acaccggaga cctcgagctg atccctgttt ccacgctgga
      241 ggaagccgtg gaggcgctcg acccatcgga aacgacggcc tgagctaggc tgctcacgat
      301 gtccaccacc accgtacgga tcccgtaccc gaggcgccgc tggcccgtgg tcgccgtcgg
      361 cgtcctggtc gtgggcttcc tgatcctcac gttcctctcg aacttctaca tcgacgtcct
      421 gtggtatcgg gaggtcgacc tctcccaggt cttctggacc cggatctggg cccaggccgg
      481 cctggcgtcg gcgtccttcg tcacgttctt cgtcctgctc ctcgccaacc tcatcgtgac
      541 ccgccgtctc gccccgacga tcgtagccct cacgcccgag caggagatcg tcgagcggtt
      601 caaggagaac atcgagccgt acctccggtg ggcggtcccg gtcggcgcgg cgatcctgtc
      661 gatcttcgtc ggcctcgcgg ccgccgggca ctggcaagag ttcctgctgt ggcggtcatc
      721 gagcgggatc gaattcggga accccgacca ggtgttcggc cgcgatcccg cgttctacgt
      781 cttcacgctc ccgtggctga aatacctcca gagctggttg ttcgcctcgc tcgtcggtat
      841 cacggtgatc gtcgggatcg gccacttcct ccagggcggg atccgcccgc aggcggtcgg
      901 cctccgcgac aaggtggacc cgcaggtgcg ggcgcacctg tcggtgctcc tcgggctgat
      961 cctgctcgcg aagggatggg gctactacct gggtcgcttc gacctgctga cgtccgagcg
     1021 cggcgtggtc cagggtgcct cgtacacgga cgtcaaggcg cagctgcccg ctctgacctt
     1081 cctggcgatc gtcgcggtgc tgtgcgccgt gatgttcttc gtgaacgctc gctaccgcgt
     1141 ctggagcctc ccgatcatcg ccgtcgggct cctgctcatc gtgtcgatcc tgctgggcac
     1201 tgcctacccc gcgttcatcc agcagttccg ggtcaggccc aacgagcagc agtacgaacg
     1261 tccgtacatc gggcggaaca tcgcggggac ccagcgagcc ttcggactca ccgacatccg
     1321 gctggaagaa cgcccgttcg atccggtggt gacagccgac gacgtggaag cgaacgcggc
     1381 cacgatcgcg aacatccgcc tgtggcgtcc ctccgtcctg aaggagaact tcgagtcggt
     1441 ccagcggatc cgccagtact acgacttcaa cgacgtggac gtggaccgct acgagatcga
     1501 cggcgagcga cgcgtgctga tggtgtcggg ccgcgaggtg acccagagcg gcatcagcca
     1561 gcaggcccgc acctggcaga acgagcacct cgtctacacg cacgggttcg ggaccgtcgc
     1621 cgcacaggtg aactcgacca cgaccgacgg cggaccgctg ctcacgctcg aggacatccc
     1681 gcccgacgga gagccgagca cggaccagcc gcgcatctac tacggggaga tcgacgacgt
     1741 cgacttcgtc gtcgtcaact ccgccaccga ggagctcgac ttcgagggcg cgtccgggga
     1801 aacgccgttc acgtacgacg gcgagggggg gatcgacctg ggcgggtacc tccgtcgcgc
     1861 cctgttcgca tggcgcttcc aggatgcgaa cctgttgatc tccgggcagg tcgacagcga
     1921 gagccgggtc cttttccgtc gggacatcga ggacagggcg aaggcgacgc tgccgttcct
     1981 cacgttcgac cacgatccct acctggccgt gacgcaggag gggtacgtct ggatcatgga
     2041 tgcctacacg acgtcgaacg cgtaccccta ctcccagtcc gtcgacatgt cggaggtcac
     2101 cgccaacctc cttccccggc aggacgtgaa ctacatccgc aactcggtga aggccgtcgt
     2161 cgacgcctac gacgggtccg tgacgtacta cgcggatctc gacgagccga tcatgcaggc
     2221 gtggagccgc gcgttccccg gggtgttcac cccgatcgac gaagctcccg aggagatcgc
     2281 ggcccacttc cgttacccgg agaacctctt ccaggcccag gcgttccagt acgccaacta
     2341 tcacgtcgag gaaccggatg cgttctaccg gaagcaggac ttctgggagg tgccgggcga
     2401 cccgacgttg cagagcgacg tgatcggccc ggacggctcc ccgtcgcccg ctccgaacgc
     2461 gagcgcgcgc aagctcctcc cgaactacca actgctccgg ctgccgggag acaccgaaga
     2521 gcggttccat ctcgtgatcc cgttccagcc ggagaaccgc ttgaacatgg tggggtggat
     2581 ggccgcgaac tccgaccagg atggctacgg agagctcgtc gccttcaccc tgccgtccgg
     2641 ccgagacgtg gacggcccgg ggttggtctt cgcccgggtg aactccgacc aggagttctc
     2701 cgaagcgcga acgctcctgg gcaccggcgg gtcgcgggtc ctcttcgggg acctgctgac
     2761 gatccccatc gaggaatcga tcctgtacgt gctgccggtg tacgtgcgag cggccggtac
     2821 gtcggcggtg ccggagctcg agctggtgct ggtggtcaac ggatccaccg tgacgctcgg
     2881 ccgctccctg accgaggcga tccaggagtc gaccggcgcc gtcacgggcg aggaaccgcc
     2941 gccgcccgac ggcggggaag ggacggtcga gcagcagata gaacggctgt tgacgcaggc
     3001 cgtgacccac ttcgccgcgg ccgaggaggc gctccgtgcc ggggacctgg ccacgtacca
     3061 gtcggagcag gaggaagcgc gggcgctcgt cgagcaggcc aacgagctcc tcgcggccgg
     3121 gacgggtggg gagccgtccc cgtcgccctc gccctagtcg gcgggtaggc ggaacccgag
     3181 atcggcgtcc agctcggccg tgtccatctg cgccttctgg agatgccccg agtagtccca
     3241 gttcatcgcc tgccaccggg tgaactgatc gagcacccac ggtccggatt gccggctccc
     3301 gttcccggag cgtccgttgc cgccgaacgg caggtgcgcc tccgcgcccg acgtggagtt
     3361 gttcacgctc accatccccg ccgagatccg gtcgcggaaa cggaagacgt gcagcgggtc
     3421 gttcgtgtag atactgctcg acaacccgta tccgtgcccg ttcgcgaggt ccatcgcgtg
     3481 atcgaacgtg tcgaacgcgg ccacccccac gatcggaccg aacgtttccg tgcggtacag
     3541 gtcgtcctcg atcgtcacgc cgtcgacgat caccgggtgg tagtagagcc cggtctcggg
     3601 atctccgacg aagccggtcc gtggcttgtc ggccgtgatg cggccgatgc tgtcggagcc
     3661 cgaggctgtg tgatgcgggc ggatcagctc gagccaccgc tcgtaccgtt cgctgaagcg
     3721 ggccgagatc atcggcccgt acaggacgtc ctcggtggga tcgccgatcg gagcgtccgt
     3781 cgcggccttc gtgaaccggg ccatgaagtc gtcgtggacg tcgcgatgca cgatcgccgt
     3841 gccgagcgac gtgcagcgct ggccggcggt cccgaaaccg ctgaacagcg cgccttccac
     3901 tgcgaggtcg aggtcggcat cgggcatcac gacgagtgga ttcttgccgc cgagctcgag
     3961 acacggcgac tggaggtgac ggccgcacag ctccccgatc cggctgccca cctcggtcga
     4021 gccggtgaac ccgaccttgt cgagtacgcc gtcccccaag gcgcgttcga tcccctcgaa
     4081 cgtctgcggt ccatcggcca gcacgagctg gaaggctcct cgcgggaatc ccgcgtggag
     4141 gatcagctgg gcgaatgcct cggcggtggc gggcgtgtac tcggcgggct tccagaccac
     4201 ggcgttcccg cacagcaggg cgggcacgat gtaccacgag gggacggcga cggggaagtt
     4261 gcccgcggtg acgatccccg cgacccccac gggtacgcgg aacgtgaaga gctgtttgtc
     4321 cggcatctcg gacgggaccg tctggccgta gaggcggcga ccctcgccga ggaagaaggt
     4381 gcaggtgtcg atgacctcct gcacctcgcc gcgcgactcg cggatcggct ttccgatctc
     4441 acgggtgatc agccgggaca gcgactcgaa gttgtcttcc acgagccggc cgagctgctg
     4501 gatcgccctg cctcgaaccg gagcggggac cccggcccag gtcggttgcg ccgccttcgc
     4561 ccgccggcag gcgtcgagga aggtggacgc gtcgccgagc tgtgcctcga cgaccgtctc
     4621 ctccaggcgg gcggggttca cgatcgtgag ggttccgccc ggggagtccc ctccggcgct
     4681 gccgtcgagc agggacgtca cggtgcgggc ggcggtcatc ctgcagcgat gctaccgaac
     4741 cccgtctggt cgtagggttc caacgcagca tccgagggta cggcccgatc gaaagggtga
     4801 tgaggacatg gcgtacgtga tcgcggaacc ctgcatcgac gtcaaggaca aggcctgcgt
     4861 ggacgagtgc ccggtcgact gcatctacga ggggccgcgg atgctctaca tccatccgga
     4921 cgaatgtgtg gactgcgacg cgtgcgtgcc cgtctgcccc gtcaccgcga tcttccccga
     4981 ggacgatctg ccgtccgaat ggaagcagtt cacggcggtc aacgccgaat ggttcgagac
     5041 gaccggtctc ggctccccgg gtggtgccgc gcaggcgggt ccacagccca aggaccacgc
     5101 gatcgtcgag ggctgggaag ccgtctaggg ctcctcccgg gcgcgcggca gcaaggtcag
     5161 gagtgcgatc ggcgctccga ggaggagcca catcgccgag tgcagccccc accgttccgc
     5221 gacggaagcg atcgcgagtg gaaggaggac tccgacgagg tccgcggggg ccccgaccgc
     5281 gatggcggtg cctcctcgcg ccgggagctc ggagtacagc cgggcctgga cgatcgcgta
     5341 ccacccggac ctgaccagac cgagcggaag caacaggatc aacttcccgg ccggggaccc
     5401 gaccacgagg aacgccggat agaccacgag cgcgcccatg gccgaccacc gcagccatga
     5461 aaggccgtcc atccgccgaa ggatccggat cacgagggcg tcacccagca gcgaggccac
     5521 ggacaggatc ccgacacccg ccgcagcgac gctgggtgag gcgcctccct cgtcgaccag
     5581 atacagcgcc gagaacccca cgaacacatc gcccaagagg tcagtgagct gtaccagcgt
     5641 cagccacctc agcaccctgc gctcccgcag cgcggcgatc gcgccgcgga ccgtcgcacg
     5701 gagatgcgcg gtgtcagggt ggggcgccgg gaagcggaga cgccgcgcgg acagcaggac
     5761 gggaaccgtc atcagcgcag cgaccaccgt cgtcccgcgc cagccggatc cgagtccgac
     5821 gacgccgacg aggagaaggg ggcccaagat cccgccgacg gaaccggcaa ccacccatct
     5881 cgccatgttc cgctccgtcg atgccggctc gaggtccatc cacgtcgctt gggcgaggct
     5941 cacgaacgcg ccggacgccg ggaacagcag gcaggccgcg gccaacagga gaccgaagcc
     6001 tgacgccgcc gccgttccga tcagcgccag gacgaacgcg acaccaccgc ccagcaccac
     6061 ccggcggcgc catccgatgt cggagaggag ggcgaggccg ggctcgacga gcgtgccggc
     6121 gatcccgggg atcgacagca gcagcccgat ctggaggtag gagagcgaga gctcccgacg
     6181 gatcagtggc cacgcggctt cccggaggcc gaacaccaac tcgtccatga actcgatcgc
     6241 gaggaagatc gcgaccgagg cgagggctcc cgggcgaggt cagccgaagg ggcggggcac
     6301 gcgcatgatc cgaaggctac cgcacatgcg cacaaggtgc ggcaggatgg cggcgcatgg
     6361 atcggccgaa ggatgaccag atcgccctcg cgcggcggct gctcctcttc gttgggctgg
     6421 cgttcctcct cgcccagctc ctcgcgttct cgctccaccg cccgccgagc tgggacgagg
     6481 cgatctacct gtcgcaggtg gctcccgggg ccgaggcgct ccccttcgtc ccttcgcggg
     6541 cgcgaggcat cacgttcctg gcggcccccg tgctgcagct cggcggctcg ctcgttcacc
     6601 tgcggctgtt cctcgtcgtc gcctccgcgg cggcgatgac ggcggcgttc cgctcgtggg
     6661 cgaaggtgat cggaccgggc gcggtcgcag cagccgtggt gttcgcggga gcctggccgg
     6721 cgctcttcta cggatccgag ctgatgccga acctgtgggt tgccctcatc ggcgtcgcgg
     6781 cgacggccat cctcgcccgc cggctggcga ccggcgaggg tcgatacgac gagctgctgg
     6841 caggagggct cgtcgccgtc gccgccctgc tgcgacccct cgacgcggtc gtcctcgccg
     6901 cagcgctcat cctcctgccg atcgcggtgc ggcgggcgac gatcgcctgg accgttcacg
     6961 tcgtgttggg gctcgcggcg gggtgggcgc cgtggctcgt cgagatgatc ggtcgcttcg
     7021 gctcgccggc cgaggcattc acggcagcgg cccggctcgg ccacaccggc cggtgggcct
     7081 tgctcgagaa cgtccatcag tacctcgcgc tgagcgacgg tccctcgatc ggcccgatcg
     7141 ccgagccgca catcccagtg tccgggatcc tctggctcct tgggatgacg gcgctcgtgg
     7201 tcgccgggat ccgcgcggcc gcgggcgccc gtctcctccc gtcgctggtc gtgccgaccg
     7261 cgacgggtgc cgcgctcgcg gccgaataca tcgtgttcac cgacgcacag gcgccccggt
     7321 tcctactgcc ggcgctggcc ctgctggcca tccccgccgg gctgggattc gtgtggatcg
     7381 cggatcgggc tcgagaccaa cgcccggctc ggacgggccg atgggtcgcc gccgcggcgg
     7441 cctcgaccct cgtcgttgca tgggcgttcc tccaggtagg gatcgcatcg aggctcgagg
     7501 cgggcgtgac tcagcagcga gcctcggccc aacgggctgg ttcccgggtg cgtgcgctcg
     7561 ccgggccggg gccgtgccac gtgtacagcg aggccagctt cccgatcgtc gggtacgccg
     7621 ccggctgcct cgcggccccg atcggtaacg tcgtccgagc gtggccggag cgagcggcaa
     7681 ggctcgagcg cgacggcgtc cggccgttcc tggtccttca ccgggccgag cagccggctg
     7741 tgccgaaggg agcgtcaccc ctcgcgaaga tcccctcgga cggtcagtcc tcctcgtggt
     7801 tcatctacgg caccggctga tccgccggat ccacatcggc ggctcggatg gtgcggcggc
     7861 cgggcgacga ccatcgcatc acgacgatcc acaccaactc gctcgcgatg atcgccggca
     7921 gcacgagcca cggtgatgcg agccacgcga gcgccgcgac cacgatccag ctggacgccc
     7981 ggatgggcca cgcgagcttg cccgggtaga tggatagggt cgcccgacgc aagacgtggc
     8041 tgatccggcc cgcgcgcgtt tggacctcga gaggtctcct tgactccttc aaccgctggg
     8101 agcgagtgcc ggccggtcgg atcacgaacg atggcccttg ggggacggtc cggcggtcga
     8161 gctccgcgac gatcgcgcgg aggaccgggt tcgtgcgccc cccgaacgat agggtggtcg
     8221 tcgtctcctc cacgggcccc accgcgtcgt cccacgtcca ccacaggaac ctccgcgcgg
     8281 ggacccacgt gatcctcgtt aggggatgat gggtgaaccg gaggatgggt cgcccatcat
     8341 ggtgtacgcc ctcggaccat ccgacgatct gatcgaatgc cactggatcg agcacccagg
     8401 tgcgccctct cgacggatcc cgtagctgcc gggcagtgag gatccgccga tccgtgacga
     8461 gtgggtgagc gccgctccgt gcgatcaccc gttcccccgg ttcaagcgat gactccagga
     8521 aggcgtctcg atcggagagc cactggcgcc atcgcgcgat ggccctctct cggcggcggc
     8581 gccgctccgc acggcttcga ccggcgccgg gctccggatg caccggctca cccatgttgc
     8641 gaggctagct ccaaggcgcg gtggatgcgt atcggcgcgc accacctctc acctgtcatc
     8701 tcccgctcgc gtcgctatcc tgggtatgcg gggtggagca gtctggtagc tcgctgggct
     8761 cataacccag aggtcacagg ttcaaatcct gtccccgcga cgaagccgag acggcccgct
     8821 agcagggcga acggtgcgag gcccctgagc ccgagagggt tcgggggcct cgctttttcc
     8881 cggactttcc cggttctcag gagtcagccg gctgactcgt tccctctgcc ccagggcctc
     8941 gccgatccgg tcagccgcgg cgagctcctc ctcgccggag ggttggctat acacgtcgag
     9001 ggtgaagccg gtggtggcgt ggccaagggc gcggctcacc agatcggccc ggacgccgga
     9061 ggccaacgag agggtggcga aggtgtgccg agctccgtga agtacgagct cgcggacgcc
     9121 ggcggctttg gccagccgca cgaagcggtc gcggagcgtg tcggggtgaa cgacctcacc
     9181 atcggcttcc gtcccgatcc aggccgcatc gagcccgaga ccgccgtcgg catgccatgc
     9241 aggcccgaaa gcgagccggt cctcgttctg ggcggccttc caggccttca gcgcgtccct
     9301 cgtcaccccg tcgaagcgga tcgtccgcgg acgtcgtccc ttcgtcgtcg cccggatgta
     9361 gagcctcggc gcagagctcg aggatcgacc gcggacgagc acttgccggc gcaccttgag
     9421 cgatcgcgcc tctaggtcga tgtcgtccca ccagaggccg atgagctcgc cgcgccgaag
     9481 accggtcgag agcgcgaggc gccagatcgc ggcgcgatcc cggtcggcgc gcgccacgaa
     9541 cgcccggacc tctgcgaggg tccatgcctc ccgttctgtc ctcgggactc taggtctctc
     9601 cgccagcgtg gccacgttca tcggaacgag accacgacga gcggcatcgc cgagcgcctt
     9661 cgagaggagc aggtgcgcgt tcgcgactgt cttggtcgag agcggctgac ctccgcgacc
     9721 tccgtcttgg agcaggccgg cgtacaggcg ctcgaggtcg gcggccgtga gctgctgcag
     9781 cgggatgtct ccgatccggg gcgagatcca cgactggatc gccaggcgta gcccttcgag
     9841 agtcgatggc gcgagggcgc cggaggcctc gcgcgccggg agccattcct cgatgcgggg
     9901 ctactggccg atgggcggct cgtcgaaagg gagcgctaca acgtcaggcc ggagacgacc
     9961 ggctactcgg tctcggtgga gccccgggac ctctagtgac atccgcgaac actcgcgcat
    10021 cacggtgcgc ccattcccgg ataccatcca cggtggtggc cgaagggacg atcatccgtg
    10081 agttccgcaa ggacgacctc ggggcgatca ttgagttgtc gctgcgcgcc tgggaacccg
    10141 tattcgcgtc actgcggaag gtcctgggcg acgacgtctt tctccgcttg cacccagtct
    10201 ggaaggaaga ccaggctgac gaggtccgat ccgcgtgcac caacgaacaa cgggacgtgt
    10261 tcgtcgcggt gactgacggc cggccggtcg gtttcgtcgc ggtcgcactg aacgtcttcc
    10321 ataacgggat gggcgcaatc gacatcatcg gcgtcgatcc tgactatcaa cggcgtggca
    10381 tcagctcgcg gctgaccgag ttcgcgactg agcacatgcg cagccgtggc atggacatcg
    10441 ccatggtgga gaccggcggt gacccgggac atcaacctgc acgggcggcg tacgaggcag
    10501 cgggattcac gctcctaccg attgccagat acttccggct actcgactga ccaaggcccc
    10561 cgtatccccg acgctgacgc tctgcgttgc taggggcctc caggaacatt cgagaacact
    10621 cgcgcatgcc gacgtgcctg ccctcggtga gcgatggtgt caggcttgcc ctgatgaccg
    10681 agacacccac actgcacttg actgtcggcc tgcctggtgc cggcaagacg acccttgctc
    10741 gaaagatcgc gcacggggac aaggcaattc ggttctcacc cgacgagtgg atgattcccc
    10801 tcttcggaac gacgtggcgt gaacccgacg ccgtcggcaa gcgagacgcc cttgaaggtc
    10861 gcctgttgtg ggctgcgcac gaggtgctcg gcgtcggtgg ctctgtggtc ctcgactttg
    10921 ggtgctggac agaagcagag cggtgggcca tacgtgcagt cgcggaacat gcgggcggac
    10981 gttttcgcct gcacttcatc gacctgccgg aggcggaacg acgtgcgcgc gcaacgactc
    11041 gctggcaggg ggacccgggc tccaccttcg agatgagccc acgcgaccac gttcgtttct
    11101 taggcctctt ccaggccccc acggaagcag agatcgctgc ggaacacctg cctgcgccgc
    11161 ccaggggtca caggtcatgg ctcgcatggg ctgccaaccg ctggccgagc cttcccgact
    11221 tcgctgacga gcaggagtag gcacgctcac tgctctgggt acatccatca actctcgggc
    11281 agaacaacgt tcccccatct gagggtgaag tcgggtccac gaggctccac gggcgccact
    11341 gggtcaaccc tgatactgca ggtcagagcg ctgagcgaca tcggactgcg cggcgtgccc
    11401 cgagccggta tgggctcata agccagcggc cacaggtcaa atcctgtccc cgcgacgagg
    11461 agtacgaggc gacacgaagg gccggcccgg ccggcctaca ttcctcggcc gcgtcgcaga
    11521 gcgggatcgg agcaaacggc tggttctcat ctccggcccc ggcgatgccg atgacgtccg
    11581 agatggactc cttcgcaccg gtgcagctcc ccgacgacat ccagcggcgt ctgaacgtgg
    11641 ccaaggcagc gggcgcgctc gcgctgatcg cggtctgggg ttggttcgcc ctcgtcaagg
    11701 gggatcagac gcccgtgttc gtgtatctga acatcgccgt ccacgaggtc ggtcatgtcc
    11761 tcttccggcc gttcggggag ctcacgatgc tgatcatggg atccggtttc gaggtcctgt
    11821 tcccgttcgc ggtcggcgcg tacttcctcc tccgcaagcg cgacctcgtg tccacggccg
    11881 tcgcctgggg atgggcggcg agcgcccagg ccagtgcggc gacctatatc ggagacgcgg
    11941 acgacggccg actggctctc ctgggagcca ccgggccgga tgcggccggt gactgggaac
    12001 ggatcctggg cgtcgagttc ttcgacaagg tctacctcgc cgacaggatc gccgggcccg
    12061 tacggaccgt gggcttcgtg ctctggggtg tggcgctctg cctcgccgtc tgggcgatcg
    12121 tccggaatcg tcagctcgtg cgggcggccg gagcaagtcc gaagccccgg gtgaccgcgg
    12181 agcctcgagc gccgctcgtc cccatccacg acgaggagat gtggcgctga tccacgcgtg
    12241 cgggccctcg ctcgagttgg agtacgatgc tccggcccga acccgaggga gcgtcgtatg
    12301 gctggaagca ccgtcctcat cgaatggctc aggcaggtgc cgctgttctc cgcttgctcg
    12361 gctcgcgagc tccgtgcgat cgcgggcgtg gtgaaggagg tcgaccaccc gcgggggacc
    12421 gtgatcgcca ccgaggggga tcccggcgtc ggactgttcg tgatcgtcga cggtgaggcc
    12481 gaggtcacga tcgggggcaa gcggatggcg atcctccgtc gtggggactt cttcggtgag
    12541 atcgcgttgc tcgacggggg ccctcgttcc gccaccgtgg ccgcccggac ggatgtccgg
    12601 ctcctcggcc tgaccgagtg ggtcttccgg ggactcctcc aggagcaccc atcgatcgcc
    12661 gtcaagaccc tcgagtcgat ggccggccgg ctccgcgacg cgacgcaagc cgcgacggca
    12721 tagccctccc cggccgatgc cggtcctggc ccgcccggac ggcgtccgcc tgtaccacga
    12781 gctgcacggg tcagagctcg cccctccgct gatcctgctc gagggcatgg gcggcgacat
    12841 cccaggttgg cgccggaaca tcccagtcct cgcgcgcgag ctccgcgtca tcgcctacga
    12901 cttccgcggc aacgggaaca gcgacgagcc tcccggccgg gtgacgatgg cgacgttcgt
    12961 cgaggacacc ctcgccctgc tggatgcgct cggggtgggc cgggcccacg tctacggcca
    13021 gtccttcggc ggcatggtgg cccaggagct ggcgctcacc cacgtcgagc gcgtgcgcac
    13081 gctgatcctg gggtgcacgc acgccggacc gcgacacgtt ctgcggggga agcgatccca
    13141 cgtaccgaag ggagagccgt ggcgctccct ctacgcgccc ggcttccccg acctccaccc
    13201 cgagcatgtg gccgaggatc tccgggtggg tgccgcccag ccgaaacatc cggagggcgg
    13261 gcggagacaa tgggaggcga tgcaggggtt cgacagtctc gaccgcctcc ggaacctcaa
    13321 ggtccccacg ctcgtcgtgc acggttccga ggatcaggcg atcgcaccgg agaacgcgga
    13381 gctcctcgcg gagcggatcc cgggggcgga gttgctgctg ctcgagggtg ccggccacct
    13441 ctatcactcc gagcgggccg gaaaggcgga cgcggcggtg ctcgacttca tccggaggca
    13501 cgacgatgcc tgacgcaccc gaagaggtcg ttcggctggc tgaggagcgg gcagatgcgc
    13561 ggagctctcg ggacttcgcg gcggccgacg cgctccgcga tcggatcgcc ggatcgggct
    13621 ggaccgtggt ggacgacccc ggtggatacc gactggagcc gatctcaggc agcgcgacgg
    13681 agcttccggc gcgacgtcgt tccgccgagg tcccttcgat cttgcaggac gctcccgaca
    13741 tggatgcgac cgtccactgg gtctgtgagg gctggcacga ggacatcgag cgggcgatcg
    13801 ccgccttccg cgcgcacgag ggcggtcgat cggtccagta cgtggtggcg gacgtgaccg
    13861 gccgagatcc gacgacctgg ggcgagggtg tcgacgtcgt gtggttgaag caggggaccg
    13921 gctgggcggc ggcgaggaac gcgggcatcc ggcgcacccg cgggcgcatc gtgctgatgg
    13981 ccgacggctc ggtcgagccc acgggcgacg tgttcgggcc gctcgaggag gcgctcgagg
    14041 atccgggggt cggcgtgtgc ggtcccttcg ggatcgtgac acacgacctg cggcagttcg
    14101 acgagtcgcg caccggagac gtcgacgcga tcgagggcta tctgatggcg ctccgtcgag
    14161 agacgctcgt cgaagcgggg ttcttcgacg agaagttccg ctggtaccgc acggccgaca
    14221 tcgagttctc gttccgggtg aaggacctc
//
LOCUS       DASUYD010000109         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1819804, whole genome shotgun sequence.
ACCESSION   DASUYD010000109 DASUYD010000000
VERSION     DASUYD010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1819804"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1044
                     /locus_tag="VF195_05915"
     CDS             <1..1044
                     /locus_tag="VF195_05915"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX6580387.1"
                     /translation="QYRFPARTIAAGDEASPLPAGDEIDPPAPTSDTEDDRAFDEFLR
                     DTGTLGFVVLDDDQLVYERYFGADRQTRQTSFSVAKSFVSTLIGIAIDEGLIGSVTDP
                     VTEYLPELSERDPRFERITLRHLLTMSSGIRYEEQSLPLPWGDDVDTYYGTDLRDLGL
                     AETEIVSPPGGGWLYNNYHPLLLGLVLERATGMSVSEYMATKLWQPLGAEANATWSLD
                     SEDSGFEKMESGLNATPVDYARFGQLFLHDGEWNGTRIVSKDWVREATTTDTSDAPAG
                     NYYFADDYQYFWWIDTVRQGRFYALGNFGQYIYVAPDAGVVIVRNGRDWGVDNDTWLS
                     VFREIADELLERS"
     gene            1194..1541
                     /locus_tag="VF195_05920"
     CDS             1194..1541
                     /locus_tag="VF195_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580388.1"
                     /translation="MKNTSDGVGRGGVMQAPRRTVHDLANHALDPERRGVSESLAWAE
                     KVFHDQEMPPDELRVLLTTADPELVHRHVELHLERLEEWLIMQRRSLTVAEGILTEAA
                     GRRRGLKMSLRKR"
     gene            complement(1602..>2370)
                     /locus_tag="VF195_05925"
     CDS             complement(1602..>2370)
                     /locus_tag="VF195_05925"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX6580389.1"
                     /translation="PWLRDRPLTVIRAPDGVDGQRYFQKDTPKHAPSWIRTVTIPAPS
                     AKRDVAYTVCNDAATLAWLGNQAALEFHPAPVRRDRLERPDLLVVDIDPPDDAFDAAV
                     EVAFLVLEVLDDLGMTTLVKTTGGKGLHIVAPIERHVSAERLRQAATRLTTIVSERRP
                     DLVTSEFRKAGRKGRVMLDPSRNGTGATIVAPYSPRVRAEATVSFPVLPDELRSITPG
                     EFTVATVPRLLDGAAPARWTAAASGRRQRLPASLLGD"
BASE COUNT          398 a          756 c          833 g          383 t
ORIGIN      
        1 cagtatcggt tcccggccag gaccatcgcg gccggtgacg aggcaagtcc gcttccggcc
       61 ggcgacgaga tcgatcctcc tgcgccgact tcggacacgg aggatgatcg agcgttcgac
      121 gagttcctgc gtgacaccgg cacgctcggg ttcgtggttc tggacgacga ccaactggtg
      181 tacgagcgtt acttcggcgc cgaccgccag actcgtcaga cctcgttctc ggtcgcgaag
      241 tcgttcgtgt ccacactcat cgggatcgcg atcgacgagg ggttgatcgg aagcgtgacc
      301 gatccggtga ccgaatacct ccccgaactc tccgaacgcg atccgcggtt cgaacggatc
      361 accctgcgcc acctgctcac gatgtcttcg ggcatccgct acgaggagca gtcgctcccc
      421 ctgccctggg gggacgacgt cgacacgtac tacggcaccg acctacgtga cctcggcctt
      481 gccgagaccg agatcgtgag cccacccggc ggggggtggc tctacaacaa ctaccaccca
      541 ctgctgctcg gtctggtgct cgagcgcgcc accgggatgt cggtctccga gtacatggcc
      601 acgaagctgt ggcagcccct cggagccgaa gcgaacgcga cctggagcct ggactcggag
      661 gactcgggct tcgagaagat ggagagcggc ctgaatgcca cgccggtcga ctacgcccgg
      721 ttcggccagc tgttcctaca cgacggcgaa tggaacggca cgcggatcgt ctcgaaggac
      781 tgggtgcgtg aagcgacgac cacggacacg agcgacgcac cggccggcaa ctactacttc
      841 gccgatgact accagtactt ctggtggatc gataccgtgc gacagggtcg cttctacgca
      901 ctcgggaact tcgggcagta catctacgtg gcgcccgacg ccggggtcgt catcgttcgg
      961 aacggtcgcg actggggggt cgacaacgac acgtggctga gcgtgttccg cgagatcgcg
     1021 gatgagctgc tggagcgatc gtgacctggt gccgaccgtg atggcgggga tcgcccttct
     1081 cgagaaggag gttgagggat ggctgtctgg ctcgtattgg ctctgctgat cctgctggac
     1141 gcggcagcct ggctgggggg ccacgactcc cgcgatgggc gggactggac atcgtgaaga
     1201 acacgtcgga cggtgtggga cgaggcggcg tgatgcaggc accccgccga acggttcacg
     1261 acctcgcgaa ccacgcgctc gatcccgaac gccggggagt gtcggagtcg ctcgcttggg
     1321 ccgagaaggt atttcacgat caggaaatgc ctcctgatga gcttcgcgtc ctcttgacga
     1381 ccgcagaccc cgaactcgtc catcgccatg tcgagctcca tctggagcgc ttggaggaat
     1441 ggctcatcat gcagcggcga agcctaacgg tggccgaagg gatcctgacc gaagccgcag
     1501 ggcggcgcag gggcctgaaa atgtctctac gcaaacgatg agaatgctgc acgtcgaccg
     1561 gagtagcgtc cagttgcatc gatgaggagt gcgagtgcgc gtcagtcgcc gagcagcgac
     1621 gcaggcagcc gttggcgtcg cccactcgcc gccgcggtcc agcgcgcggg tgcggcgcca
     1681 tccagcaggc ggggcaccgt cgccacggtg aactcgccgg gggtgatcga ccgcagctcg
     1741 tcggggagga cggggaacga gaccgtggcc tcggcgcgaa cgcgaggcga gtagggggcc
     1801 acgatcgtgg cgccagtgcc gttgcgtgac gggtcgagca tgacgcggcc cttgcgaccg
     1861 gccttgcgga actcggaggt cacgaggtcg ggccgccgtt cgctgacgat cgtggtgagc
     1921 cgcgtcgcgg cctgccggag ccgttcggcg gacacgtgcc gctcgatcgg cgcgacgatg
     1981 tggaggccct tgccgccggt ggtcttgacc agagtggtca tgcccaggtc gtcgaggacc
     2041 tcgagcacca ggaacgcgac ctcgaccgcg gcgtcgaacg cgtcatcggg cgggtcgatg
     2101 tccacgacga gcaggtcggg gcgctcgagc cggtcccggc ggaccggcgc cggatggaac
     2161 tccagcgccg cctggttccc cagccacgcg agcgtggcgg cgtcgttgca caccgtgtac
     2221 gccacgtccc gcttggcgct cggcgcgggg atcgtgaccg tccggatcca cgacggtgcg
     2281 tgcttcggcg tgtccttctg gaagtaccgc tggccgtcca cgccgtccgg cgctcggatc
     2341 acggtgagcg gtcgatcgcg cagccagggg
//
LOCUS       DASUYD010000110        13039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1379686, whole genome shotgun sequence.
ACCESSION   DASUYD010000110 DASUYD010000000
VERSION     DASUYD010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13039
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379686"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /gene="cysS"
                     /locus_tag="VF195_05930"
     CDS             complement(<1..924)
                     /gene="cysS"
                     /locus_tag="VF195_05930"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX6580390.1"
                     /translation="MRLYNTLSRAVEEIVPVDGRLVRIYSCGPTVYRYIHIGNMRSFM
                     LGDLIRRALRFEGLDVRWVMNITDVGHMTDEVSDTGRDKMELAESDEGLSAAEIAEKY
                     TQAFLEDADLVGIERADLYPRATDHIPEMISMIEMLIEKGHAYEVDGTVYFETTTFPG
                     YGKLSGNTLDQLRSGHRTELATDPGKRHPEDFALWKKAGANRLLRWPSPWGDGYPGWH
                     IECSAMSIKHLGDRFDVHTGGNDNKFPHHEDEIAQSEGAVGHPVVSIWAHGGFLQMGG
                     QKMAKSAKNIKRVTDLAEQGIDPLAFRWFTFQ"
     gene            complement(945..1385)
                     /locus_tag="VF195_05935"
     CDS             complement(945..1385)
                     /locus_tag="VF195_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX6580391.1"
                     /translation="MDERDLAILTALQADGRATYADVGATVGLSASAVHERVRKLEHQ
                     GVIRGYRAVVDPEAVGLYVTALIAAIPLDPHQPDDLPARVREFPEVEDCYSVAGEANY
                     VLKVRTKTTGDLEDLIRRLREKAAVTTNTTIALSIPFEGRALRP"
     gene            1507..2223
                     /locus_tag="VF195_05940"
     CDS             1507..2223
                     /locus_tag="VF195_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEX6580392.1"
                     /translation="MRRRPTRPADRGDTPGPRSIPISKPGGGTRWLTVLDPADDAAYR
                     ASVGPLVGRIERSLGPEVVAVRAKRDDAGWTLAPWEAARVTWRSSIRRAILDTPGATF
                     ALADVRDCYASISLETITVLLGPEAAQAVALLHRFREGGVPGLPVGPEPSAVLANAAL
                     SHLDRAIRSAGARHLRWVDDVVLWGSADEVRTAMAAMRTAGEAIGLALNRDKTRVFED
                     REEAAVVVLGERDSSIIAAP"
     gene            2220..3719
                     /locus_tag="VF195_05945"
     CDS             2220..3719
                     /locus_tag="VF195_05945"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6580393.1"
                     /translation="MRTLYRASRVVTLSHPTLGEWILVDDRHVQRVGTGEPPDAELTI
                     DLPGTTIVPGFIDSHVHLTATGRSLANTDVHESRSKQDFLALVRGRATDGGTVVHVEG
                     FDETRWDTHELPTAHDLDAITPTPVVATRADGHISVVSSSTLAAIDLADADGVELDAE
                     RHPTGRLTKEANRRAVAWVEASYDTRKIEGFQLQAAGLAASRGVTAVHEMSLDGADLE
                     VLLGHRSRLPVDVSPTLASMSLPEAMERRLAAIGGDLPVDGSIGARTAALSHAYDDGA
                     DTGELYHDDDAMAEFFHAAHNAGLQVGVHAIGDRAIEQVLAAWERVYLALDSRERRHF
                     RARRHRIEHFVLPSADQIERTAMLGLAVSVQPAFDRLWGQGGGMYEERLGPARAMAAH
                     PIRTMLERGIEVGVGSDAPVAPLDPMLAIAALEEHHEPTQRLTRLEGLKLHRVGSARL
                     GRQDGKKGALEPGWHADFAAYDADPLDASTVEGLRPVLTVSLGREVFRD"
     gene            4033..4389
                     /locus_tag="VF195_05950"
     CDS             4033..4389
                     /locus_tag="VF195_05950"
                     /inference="COORDINATES: protein motif:HMM:NF025204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HEX6580394.1"
                     /translation="MAERTEEREDDDVGTDQELDELEPTEEDLDEAATPAAADVESIH
                     EVLEKQEARDDQDEADEEEPVVPISTTKADDRLEPIEARVVPIQATEFVCKNCYLVKH
                     RSQLADKRRMFCRDCA"
     gene            4437..6638
                     /gene="lnt"
                     /locus_tag="VF195_05955"
     CDS             4437..6638
                     /gene="lnt"
                     /locus_tag="VF195_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEX6580395.1"
                     /translation="MLSSLAFPPVAAWPLAFVAPALLLIGLRNATPARGFAIGLVYGL
                     VGFGLTLSWLQLFGTLAWSALTILAAASVALFGACLPLVRRSGHPLLSALGAAALWTS
                     IDWLRGLWPLGGFTWGSLGASQVANPVTVRLGVVAGVFGVTFAVAFAAALVVEAADAE
                     GAPLRITAIGLAVIAALGPAVIPFAEASGRQIDVATIQVDFLEGVEGRSREEGDVAVT
                     RLNLELHATLSGDPPDLAVWGESALDPGSLSILDEVRGTIADIGTPVLSGATSTDIRS
                     GEGGDALFNQAVVFDGGGAVVDVYRKTHLVPYGEYIPWKPVVGWISALEQIAYELTPG
                     ERLHTLTAPGLPRFGAPICFENSFPALGRELVRQGAEFLVVLTNNASYDRTAASSQHL
                     QMSRLRAIEDGRWIVHAAVSGISAFIDPTGRVHDETELFEPALIRHTIRASSERTWSV
                     RLGDWLPILSLAIVVSLAAVPRRRHRERPDPEPLAEGVRSLVILPTYDEAATIRDVLA
                     GVLRHPGVDALVVDDSSPDGTARIVRELMAGEPRLGLVERPARSGLASAYEEGFRRAL
                     EDRYDLIVEMDSDLSHDPAQLGALLAGARDRFHLVVGSRYVPGGSVTNWSRMRLALSR
                     VGNTYARLMLGVPVHDATSGYRVYRRQLLSDLVAEPFHADGYGFQVELVMRSWLRGWS
                     IGEVPITFREREHGHSKLSRRIVVEALWLVTRWGIALRATGRPYRGGPSAA"
     gene            6934..8997
                     /locus_tag="VF195_05960"
     CDS             6934..8997
                     /locus_tag="VF195_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HEX6580396.1"
                     /translation="MRGRRHRRRGLLGPIVALALLAGQAGILTARAAPPVDSPGPPSA
                     DGVAPVIVDTQSSNDDCGQLGFDHGISIASNGQASSGAMTVTVAGYNGPTGFADWSST
                     LPVHGVYVKGGPSGGDLFGYPAGDAGDHDLHTPQKPDGGYYSVSHLAICWNDAPAEPD
                     VSVVKVNDPGGVVQNGDSITYTLSVTNDGTGTAIDVHLADQLPVGVTFAGATAGCGEA
                     AGLVTCALGDIGAGAGVSVDITVTVDDGFCGPIANDAHVSASNETGRAAGNNDSNEVS
                     NSAECEEPSPPDLQVTKTSDAGRILHQGDSFQYTVTVTNVGDVQATGVELIDTLPSGD
                     ALSISVSPFPMFDGHPCVIASSITPGGTAQATVDCGPITLGPGDAASVTFRVAVTGEE
                     CGAITNVVDVGGTDEPTVNVGSDNHAEASDEIACAPRIGLRKGGPALAHVGDTVAYTF
                     SVTNTGGVDLRDIDLRDARCDTTPDRTDDGNGDAVLAVDERWTFACDHTIAAADGDPV
                     HNEATVTGVHDGATVSSTDTHDIDLIHPAIDLEKSASPTSGPAGTIIVYTYAVTNTGD
                     TTLFDISVDDDSVGHVGDIASLAVGQSSELTFEMTLGSSPITNVATATGSDVLGASVS
                     DVDEVTVSVVAGGAGGDGGGGTGGGSPFTGSATATLSGWAAVLAVAGSLVLLAASRRR
                     RETDT"
     gene            9251..11686
                     /locus_tag="VF195_05965"
     CDS             9251..11686
                     /locus_tag="VF195_05965"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580397.1"
                     /translation="MGTRTNSRHSRRRTKRGLASFVSFVLLFAFALGQSSLSALAEDA
                     GTDPVAAEETISDGNTGEGTVPVEEALVEEAPAEEAPAEEVPAEEVPAAEADGDASTG
                     AGAATAGAGEARKGQINVARANRTTAIAAPAAPPASELDGGDIDLDFIAAGPFTYNHA
                     TGLGTHPQFGFDDRTISKSNGVVESLEAGDFECGDYVTFFTEITVDSGAAAGGTIELD
                     YTFGAETTGQPGLGFDDIISVDINTPDDGNKNLDGDEAATLIAEGFDSSGYDQVTGTV
                     EIEGLDAGDVVIVRLIAELTCLQGETPTGNILNAIESGRVTSNGDDTIPVGQQTVPMK
                     TSGFVAEPAIDIEKTCPLEAGFGDDITYNITITNNGNENLENIVVTDTVNGNAPDDIS
                     DLFADTLAAGASDSAQYVYTPDANDPDPLPNTVTVTADGVFSSTQVTDTDTCETDITH
                     EPAIDVEKTCPLEVPFGEDVTFTITVKNTGNEPLENVSVTDALLGGDITAAFNLPDPL
                     PVGNTVYSHDFVYTPGANEDPVENSVTASGDGVDSGVTATDTDECETDILNPSIGVTK
                     SCTELAHAGDTVTYTITVTNTGDEDLDGVTVMDTILGDLSASFADELAVGASESHDFD
                     YVVLGSDPDPLVNEVTAKGSGVGSRVAVEALANCVTDLIHPAIEIVKTVDEDTVPIGT
                     TVTYTYVVTNTGDTTLYDISVDDDILGHIGDIAVLEPGESATLTKDFVVGDVPVTNVG
                     TATGEDILGRSVSSDDDAVVTPIAGATPPNPPTPFTGSDAGRLGIISMVLFGIGVTVV
                     ATTRRRRPEREAA"
     gene            complement(12151..>13039)
                     /gene="gcvPB"
                     /locus_tag="VF195_05970"
     CDS             complement(12151..>13039)
                     /gene="gcvPB"
                     /locus_tag="VF195_05970"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEX6580398.1"
                     /translation="SDARGLVDAGALAKLVDDSVAGLMLTNPNTLGLFEEEIEEIAAT
                     IHGVGGLLYYDGANLNAILGRCRPGDMGFDIVHINTHKTFATPHGGGGPGAGPVGVKD
                     ELAAFLPAPTVRRNGDGGFSLDAGGERSIGRMHGFHGNIGILVRAYAYVFLHGADGLR
                     EVSELAALNANYLARLVEEEFPLAHPGMRPMHEFVATAKPLRDATGIRAMDVAKRMID
                     LGFHPSTVYFPLVVEEAMMVEPTETESKATLEAFGEALRRTAEEARERPDLLREAPVT
                     TPVRRLDEARAARALKLRW"
BASE COUNT         2051 a         4570 c         4402 g         2016 t
ORIGIN      
        1 ctggaacgtg aaccaccgga aggcgagggg gtcgatgccc tgctcggcca gatcggtcac
       61 ccgcttgatg ttcttcgcgg atttcgccat cttctggccg cccatctgca ggaacccgcc
      121 gtgtgcccag atcgacacga ccggatgacc gactgcgcct tccgactgcg cgatctcgtc
      181 ctcgtggtga gggaacttgt tgtcattgcc accggtgtgc acgtcgaagc gatcgcccag
      241 atgcttgatc gacatcgcgg agcactcgat atgccatccc ggatatccgt cgccccacgg
      301 actcggccac cggaggagac ggttcgcgcc ggccttcttc cacagcgcga agtcctcggg
      361 gtgccgcttg cccggatccg tcgccagctc cgttcggtgc cccgatcgca actgatcgag
      421 cgtgttcccc gagagcttcc cgtacccggg gaaggtcgtg gtttcgaagt agacggtacc
      481 gtcaacctcg taggcatggc ccttctcgat cagcatctcg atcatcgaga tcatctccgg
      541 gatgtgatcc gtcgcccgcg ggtagaggtc ggcccgctcg atcccgacca ggtcggcgtc
      601 ctcgaggaag gcctgcgtgt acttctcggc gatctcggct gcggagagcc cttcatcgga
      661 ctcggccagc tccatcttgt ctctgcccgt gtcgctcacc tcgtcggtca tgtggccgac
      721 atcggtgatg ttcatgaccc accggacatc cagcccctcg aagcggaggg cgcggcggat
      781 cagatccccc agcatgaacg agcgcatgtt cccgatgtgg atgtaccggt agaccgtggg
      841 tccgcacgag tagatccgca cgagccggcc gtcgacggga acgatctcct cgacggcacg
      901 cgagagggtg ttgtacaagc gcacggacca atcctaccga cgggtcacgg acgcagagcg
      961 cggccctcga acgggatcga gagcgcgatc gtggtgttcg tggtcaccgc cgccttctcg
     1021 cggagtcgcc ggatcagatc ctccaggtca cccgtcgtct tcgtccggac cttcagcacg
     1081 tagttcgcct cgccggcgac cgagtagcag tcctcgacct ccgggaactc ccgcaccctc
     1141 gcgggcaggt cgtcgggttg atgcggatcg agcgggatcg cggcgatcag cgccgtcacg
     1201 tacagtccga cggcctccgg atccacgacg gcccggtacc cgcggatcac cccttggtgc
     1261 tcgagcttcc ggacgcgctc gtgcacggcg ctcgccgaga gccccaccgt cgcgccgacg
     1321 tccgcgtacg tggcgcggcc atccgcctgg agggccgtga ggatcgcgag atcccgctca
     1381 tccagcacgg cggcatggtc ccacacgggg gaccgaggaa tccccgcgac ggccgattgc
     1441 tcgcgcccgg cccgggcgct acggtggcgt tgcctcctcc tggtcttccc cggaggagcg
     1501 aaagcgatgc ggcggcgtcc cacgcggccc gcggacaggg gagacacgcc cggcccgcga
     1561 tcgatcccga tctccaagcc cggcggcgga acccgctggc tgaccgtgct cgatccggcg
     1621 gacgacgcgg cgtaccgagc ctcggtcggc ccgctcgtgg gccggatcga gcgttctctg
     1681 gggcccgaag tggtggccgt ccgcgccaag cgagacgatg ccgggtggac gctcgcgccc
     1741 tgggaagcgg cacgtgtcac gtggcgtagc agcatccggc gggcgatcct cgacactcca
     1801 ggggcgacgt tcgcgctcgc cgacgtgcgc gactgctatg cctcgatctc gctcgagacg
     1861 atcaccgtgt tgttgggtcc tgaggcggca caggcggttg ccctcctcca ccggttccgc
     1921 gagggaggcg tgcccgggct ccccgtcggc ccggagccat ccgccgtcct cgcgaacgcg
     1981 gccctgtccc acttggatcg cgcgatccgg agcgccggcg cccggcacct ccgttgggtc
     2041 gatgacgtcg tcctgtgggg ttctgccgac gaggtccgga ccgcgatggc cgcgatgcgc
     2101 acggccgggg aggcgatcgg cctcgctctc aatcgggaca agacgagggt cttcgaggat
     2161 cgcgaagaag cggccgtggt ggtgctcggt gagcgggact cctctatcat cgcggcgccg
     2221 tgaggaccct ctaccgcgcg tcgcgggtcg tgacgctttc gcatcccacg ctgggggagt
     2281 ggatactcgt ggacgatcgc cacgtccaac gggtcggcac cggtgagccc cccgatgccg
     2341 agctcacgat cgatctcccc gggacgacga tcgtcccggg cttcatcgac agccacgtcc
     2401 acctgacggc gacgggtcgc tccctggcca acaccgacgt gcacgagtcc cgctccaagc
     2461 aggacttcct cgccctggtt cgcgggcgtg ccacggacgg cggcaccgtc gtgcacgtcg
     2521 aggggttcga cgagacccga tgggacacgc acgagctccc gaccgcacac gacctcgacg
     2581 cgatcacgcc gacgcccgtg gtggcgacga gggcggacgg gcacatctcc gtcgtgagct
     2641 cgtcgaccct cgccgcgatc gacctcgccg acgcggacgg ggtcgagctc gacgccgaac
     2701 gacatccgac cggccgcctc acgaaggagg ccaaccgccg ggccgtcgca tgggtcgagg
     2761 cctcgtacga cacgcggaag atcgaggggt tccaattgca ggccgccggg ctcgccgcgt
     2821 cacggggcgt gacggcggtg cacgagatgt ccctcgacgg cgcggacctg gaggtgctcc
     2881 tcggtcatcg ctcccggctg ccggtcgacg tctcaccgac cctcgcttcg atgtcgctcc
     2941 ccgaggcgat ggaacggcgg ctcgccgcga tcggtgggga cctcccggtc gacggctcga
     3001 tcggcgcccg gaccgcggcg ctgtcccacg cctacgacga cggcgcggac acgggcgagc
     3061 tctaccacga cgacgatgcg atggcggagt tcttccacgc ggcccacaac gcgggacttc
     3121 aggtaggcgt gcacgcgatc ggagaccgcg cgatcgaaca ggtcctcgcc gcgtgggagc
     3181 gcgtctatct ggcactggat tcccgtgaac gacggcactt ccgcgcgcgt cggcatcgga
     3241 tcgaacactt cgtcctcccc tcggcggatc agatcgagcg gacggcgatg ctgggtctcg
     3301 ccgtgtccgt gcaacccgcg ttcgatcggc tctggggcca gggaggcggg atgtacgagg
     3361 agcgcctcgg ccccgcccgg gccatggccg cgcacccgat ccgcacgatg ctcgaacgcg
     3421 ggatcgaggt gggcgttggc tccgacgcac cggtcgcacc cctcgacccg atgctcgcga
     3481 tcgccgcgct cgaggagcat cacgagccga cccagcgact gacacggctc gaggggctca
     3541 agctccaccg ggtggggagc gctcgcctcg gacgccagga cggcaagaag ggggccctcg
     3601 agcccgggtg gcacgcggac ttcgccgcct acgacgccga ccccctcgat gcgtccaccg
     3661 tggaggggct ccgcccggtc ctgacggtct ccctcggccg cgaagtgttc cgcgactgat
     3721 cgctggtctg gtcttgtcac tgtttctcct ggtgccgggt tgctctcccg cccggccgtc
     3781 ggtaacgtcc tgcctgcgtc gccgccccgg cggcgacgcg cagcggagcc cgagaggctg
     3841 agtagagaac gcggcgcccc ggcgtcgcgg ccccaaaggt ctctcgggct tccgcgtttc
     3901 caggctccgc gctcggggaa catcctgagc ctgcgaggcg tccttcccct tcgaccgagt
     3961 cgcacgaggc acgcggggga gcgagcgcta gaatccgcgg ccggatcgag gacccgaacc
     4021 cggaggaacc aggtggccga gcgcacggaa gagcgcgaag acgacgacgt cggaacggac
     4081 caggagctcg acgagctcga gccgaccgag gaggacctgg acgaagccgc cacaccggcg
     4141 gctgccgacg tcgaatcgat ccacgaggtc ctcgagaaac aggaggcccg tgacgaccag
     4201 gacgaggccg acgaggagga gcccgtcgtc ccgatctcga cgacgaaggc ggacgaccga
     4261 ctcgagccga tcgaggcccg ggtcgtcccc atccaggcga ccgagttcgt atgcaagaac
     4321 tgctacctgg tgaagcatcg cagccagctc gcggacaaac ggcggatgtt ctgccgcgac
     4381 tgcgcctgat cgatcccagg ctgccggtcg cgctcacctt ctccctcgtc gggggcgtgc
     4441 tgtcctcgct cgcgttcccg cccgtggccg cctggccgct cgccttcgtc gcccccgcgc
     4501 tgctcctgat cgggcttcgg aacgccacac cggcacgggg cttcgccatc gggctcgtct
     4561 acggactcgt gggcttcgga ctcacgctct cgtggctcca gctcttcggc accctcgcct
     4621 ggagcgcctt gacgatcctc gcggcggctt ccgtggcgtt gttcggcgcc tgcctgccgt
     4681 tggtccgtcg atcgggccat ccgctgctca gcgcgctcgg ggcggcggcg ttgtggacgt
     4741 cgatcgactg gctccggggg ctctggccgc tcgggggctt cacgtggggc tccctcggcg
     4801 cctcgcaggt ggccaacccg gtcacggttc gcctcggcgt cgtggccggg gtgttcgggg
     4861 tgacgttcgc ggtggcgttc gcggccgccc tcgtcgtcga ggccgccgac gcggaggggg
     4921 caccgttgcg tatcaccgcc atcggcctgg cggtgatcgc ggcgctgggg ccggcggtga
     4981 tccccttcgc ggaggcctcg gggcggcaga tcgacgtggc cacgatccag gtcgacttcc
     5041 tcgaaggggt cgagggacgg tcgagggagg aaggagacgt cgcggtcacg aggctcaacc
     5101 tcgagcttca tgccacgttg agcggggatc ctcccgatct tgccgtctgg ggagagagcg
     5161 ctctcgatcc gggttcgctc tcgatcctcg acgaggtgcg gggcacgatc gcggacatcg
     5221 ggacgccggt cctcagcggg gccacctcga cggatatccg atcgggggag ggaggcgatg
     5281 ccctgttcaa ccaggcggtc gtcttcgacg gcgggggagc cgtggtcgac gtgtacagga
     5341 agacccacct cgtcccgtac ggcgaataca tcccctggaa gcctgtcgtc gggtggatct
     5401 cggccctcga gcagatcgcc tacgagctca cgccggggga gcgactccac acgttgaccg
     5461 caccggggct ccctcggttc ggtgcgccga tctgcttcga gaacagcttc ccggcgctcg
     5521 gtcgcgagct cgtccgccag ggcgcggagt tcctcgtggt cctcacgaac aacgcgtcct
     5581 acgaccgcac ggccgcatcc tcgcagcatc tgcagatgtc gaggctccgg gcgatcgagg
     5641 acggcaggtg gatcgtccac gcagccgtct cggggatcag cgcgttcatc gacccgacgg
     5701 gccgcgtcca cgatgagacc gagctgttcg agcctgcgct gatccggcac acgatcaggg
     5761 cgtcgtcgga gcgaacgtgg tcggtacggc tgggggactg gctgcccatc ctctcgttgg
     5821 cgatcgtggt cagtctcgcc gcagtgcctc ggcgccgcca tcgcgagcgg cccgaccccg
     5881 agccgctcgc cgagggcgtt cgctcgctcg tcatcctgcc cacctacgac gaggcggcca
     5941 cgatccgtga cgtcctggcc ggcgtcctcc ggcatccggg cgtggacgcg ctcgtggtcg
     6001 acgactcctc acccgacggc accgcacgga tcgtccgtga gctgatggcg ggggagcccc
     6061 ggctcggcct cgtcgaacga cccgccaggt ccgggctcgc gagcgcatac gaggagggct
     6121 tccgcagggc actcgaggac aggtacgacc tgatcgtgga gatggactcc gacctctccc
     6181 acgatcctgc acagctcggt gccctcctcg ccggcgcccg cgaccgtttc cacctagtcg
     6241 tgggaagccg gtacgtgccc ggaggctccg tgaccaactg gagccggatg cgcctggcgc
     6301 tctcgagggt gggcaacacc tacgcgcggc tgatgctcgg ggtgccggtg cacgatgcca
     6361 cgagcggcta ccgcgtctat cgtcgccagc tgctctcgga ccttgtcgcc gagccgttcc
     6421 atgccgacgg gtacggcttc caggtcgagc tggtgatgcg ctcctggctg cggggttggt
     6481 cgatcggcga ggtgccgatc acgttccgcg aacgggaaca cggtcattcg aagctctcac
     6541 ggcggatcgt cgtcgaggcg ctctggctcg tcacccgctg gggcatcgcg ctcagggcga
     6601 ccgggaggcc gtaccgagga gggccttccg ctgcatgaga taccgcacgt ccctcactcg
     6661 accgtcttcc ccgataacgt tcattatgta acttccggcg aacgcccttg tggccgctca
     6721 ctcctccatc cgacccatcg aggggggtga tcctcgttct tgcttccgcc ccaggtggat
     6781 gcgaagctcc gtccggtatc tggcccacgc atccgggtgg atggtccgcg ccttccggcc
     6841 gatgtccccg tcccccgtcc cccgtagggt gtgggccgac cggccgacag agggttccgt
     6901 gccccggcgg gtgtgggaag accaggaggg aagatgaggg gacggaggca ccggcgccgg
     6961 ggactcctgg gaccgatcgt ggcgttggcg ctcctcgccg gtcaagcggg gatcctgact
     7021 gcccgagcgg caccgcccgt ggactccccc ggcccgcctt ccgccgatgg tgtcgctccc
     7081 gtcatcgtcg acacgcagtc atcgaacgac gactgcggcc agctcggctt cgatcacggg
     7141 atctcgatcg cgagcaacgg ccaggcgtcg agcggcgcca tgacggtcac cgtcgcgggc
     7201 tacaacggtc cgacagggtt cgcggactgg tcttcgaccc ttccggtcca tggcgtctac
     7261 gtgaagggcg gaccgagcgg cggcgacctg ttcggctatc ccgcgggcga tgccggcgac
     7321 cacgatctcc acacgccaca gaagccggac ggcgggtact actcggtgag ccacctcgcc
     7381 atctgctgga acgacgcacc ggccgaaccc gacgtgtcgg tcgtgaaggt gaacgatccg
     7441 ggcggcgtcg ttcagaacgg cgactcgatc acctacaccc tgtcggtgac caacgacggg
     7501 accggcaccg cgatcgacgt gcacctcgcc gatcagctgc cggtgggtgt cacgttcgcc
     7561 ggcgccaccg cgggctgtgg cgaggcggcc ggcctggtca cctgcgcgct cggtgatatc
     7621 ggtgccggtg cgggcgtctc ggtcgacatc acagtcacgg ttgacgacgg cttctgcggt
     7681 ccgatcgcga acgatgcgca cgtctcggca tcgaacgaga cggggcgggc cgccgggaac
     7741 aacgattcga acgaggtgag caactcggcc gagtgcgaag agcccagtcc gccggatctc
     7801 caggtcacga agacgtcgga cgccggtcgg atcctccacc aaggagactc cttccagtac
     7861 acggtcacgg tcaccaacgt cggcgacgtg caggccaccg gcgtggagct gatcgacacg
     7921 ctcccctcgg gggatgcgct gagcatctcg gtctcgccct tcccgatgtt cgacgggcac
     7981 ccgtgcgtca tcgccagttc catcacaccc ggcggtaccg cccaggcgac ggtcgactgc
     8041 ggccccatca cgctcggccc gggcgatgcc gcctcggtca cgttcagggt cgccgtgacg
     8101 ggcgaggagt gcggtgcgat cacgaacgtc gtcgatgtgg ggggcaccga cgaaccgacc
     8161 gtgaacgtcg gttccgacaa ccacgccgag gcttcggatg agatcgcgtg cgcgccgcgt
     8221 atcgggctcc ggaagggcgg cccggccctc gcgcacgtgg gcgacaccgt ggcctacacc
     8281 ttctccgtga ccaacacggg aggcgtcgat ctgagggaca tcgacctgag ggacgcccga
     8341 tgcgacacga ctccggatcg tacggacgac ggcaacggcg acgccgtcct cgccgtggac
     8401 gagaggtgga cgttcgcgtg tgaccacacg atcgcggcgg ccgacggcga ccccgtccac
     8461 aacgaggcca ccgtcaccgg ggttcacgac ggagcaaccg tctcttccac ggatacccac
     8521 gacatcgacc tgatccatcc cgccatcgac ctcgagaagt cggcgagtcc gacgtcgggt
     8581 ccggccggaa ccatcatcgt ctacacctac gccgtcacga acaccggcga cacgaccctc
     8641 ttcgacatct cggtcgacga cgacagcgtc gggcacgtcg gcgacatcgc ttctctcgca
     8701 gtgggccaga gcagcgagct gacgttcgag atgaccctcg ggtcctcccc catcaccaac
     8761 gtcgccaccg cgacgggctc cgacgtgctc ggcgcatcgg tgagcgatgt cgacgaggtg
     8821 accgtctccg tggtcgcggg cggcgcgggt ggagacggcg ggggcggaac gggtggcggg
     8881 agccccttca cgggctccgc cactgccacc ctctcgggct gggccgcggt gctggcagtg
     8941 gcgggatcgc tcgtgctgct cgcagcgtcc cgcaggcgtc gcgagaccga cacctaacga
     9001 tctggccctc ctctgcgagc cttcccccgg tgttctcgct cctgtcttcc gcgatcagca
     9061 ggtcttcctc cacactgact cctcccaacg gactactccg tccgcttgga gcggaggggg
     9121 ccgttcgccc gatgtgtccc ccggatggag gggttacctt tctgggcaag aggaccacgg
     9181 tgtacgccgt agggcgtcga ccgggtccat agggcaacga gaagcacaga caccagttgg
     9241 ggggtggagg atgggtacca gaacgaactc tcggcacagc cggcgccgaa ccaagcgcgg
     9301 gcttgcctcg ttcgtctcgt tcgtgctcct gttcgcgttc gcactcggac agagctcgct
     9361 gagcgcgctg gcagaggacg ccggcaccga tcccgtcgcc gccgaggaaa ccatctcgga
     9421 cggcaacacg ggggaaggca ctgttcctgt cgaggaggcc ctggttgagg aggctcccgc
     9481 ggaggaggct cccgccgagg aggttccggc ggaggaggtt ccggcggctg aagccgacgg
     9541 ggacgcctcg acgggcgcgg gagcggccac agcgggggcg ggcgaagccc ggaagggtca
     9601 gatcaacgtc gctcgcgcca accgcaccac agcgatcgct gctccggcgg caccgcccgc
     9661 gtcggaactc gacggtggcg acatcgacct cgatttcatc gcggccgggc cgttcacgta
     9721 caaccacgcc accggtctcg gcacgcaccc acagttcggg ttcgacgacc ggaccatcag
     9781 caagtccaac ggcgtcgtgg agtcgctgga ggccggcgac ttcgagtgcg gggactacgt
     9841 gaccttcttc acggagatca ccgtggactc cggcgccgcg gcagggggca cgatcgagct
     9901 ggactacacg ttcggcgccg agacgaccgg ccagcctggg ctcggcttcg acgacatcat
     9961 cagcgtcgac atcaacacgc cggatgacgg gaacaagaac ctcgacggtg acgaggccgc
    10021 gacactgatc gccgagggat tcgactcctc gggctacgac caggtgaccg gcacggtcga
    10081 gatcgagggt ctcgacgccg gcgacgtcgt gatcgtccgg ctgatcgcgg aactcacttg
    10141 cctgcagggt gagaccccca ccgggaacat cctcaacgcg atcgagtccg gccgggtgac
    10201 gagtaacggc gacgacacga tcccggtcgg gcagcagacg gtcccgatga agacctccgg
    10261 gttcgtggcc gagcccgcga tcgacatcga gaagacctgc ccgttggagg caggcttcgg
    10321 agacgacatc acctacaaca tcacgatcac gaacaatggc aacgagaacc tcgagaacat
    10381 cgtcgtcacc gacacggtga acgggaacgc tcccgacgac atctcggacc tcttcgccga
    10441 cacgctggcg gccggcgcat cggactcggc gcagtacgtc tacacgccgg atgcgaacga
    10501 cccggatccg ctcccgaaca cggtgaccgt cacggcggac ggcgtgttct cgagcaccca
    10561 ggtcaccgac accgacacat gcgagaccga catcacccac gagccggcga tcgacgtcga
    10621 gaagacgtgc ccgctcgagg tgccgttcgg tgaggacgtg accttcacga tcacggtgaa
    10681 gaacaccggc aacgagccgc tcgagaacgt ctcggtgacc gacgccctgc tgggcggcga
    10741 catcacggcg gcgttcaacc tgccggaccc gctgccggtc ggcaacaccg tgtactcgca
    10801 cgacttcgtc tacacgccgg gcgccaacga ggacccggtc gagaactcgg tcaccgcttc
    10861 cggtgacggc gtggactcgg gtgtcacagc caccgacacc gacgagtgcg agaccgacat
    10921 cctcaacccg tcgatcggcg tgaccaagag ctgcaccgag ctcgcgcacg cgggcgacac
    10981 ggtgacctat acgatcaccg tgacgaacac gggtgacgag gacctcgacg gcgtgaccgt
    11041 catggacacg atcctcggag acctgtccgc gtccttcgcg gacgagctcg cggtgggtgc
    11101 gtccgagtcg cacgacttcg actacgtggt gctggggagc gatccggacc cgctcgtcaa
    11161 cgaagtcacc gcgaagggct cgggcgtcgg cagccgcgtg gcggtggagg ccctggccaa
    11221 ctgtgtcacg gacctgatcc accccgcgat cgagatcgtg aagacggtgg acgaggacac
    11281 ggtgccgatc ggaacgacgg tgacctacac ctacgtcgtc acgaacactg gcgacaccac
    11341 gctgtacgac atcagcgtcg acgacgacat cctggggcac atcggagaca tcgcggtcct
    11401 cgagcccggg gagtcggcca cgctgacgaa ggacttcgtg gtgggtgacg tgcctgtcac
    11461 caacgtcggc acggcgaccg gcgaggacat cctcggccgg tcggtgagct ctgacgatga
    11521 cgccgtcgtc actccgatcg ccggcgcgac cccgccgaac ccgccgaccc cgttcacggg
    11581 atcggatgcc ggacggctgg ggatcatctc gatggtgctc ttcgggatcg gcgtgaccgt
    11641 ggtcgcgacc acccggaggc gccggccgga gcgcgaggcc gcgtagccgt cgaccggtac
    11701 ggaaggaacg gggcggggtc ccatcgggac cccgcccctt cctacgtctc cggcctcacc
    11761 cgacgaagat cagcgtcacc cagaggaacc cgccgtgcgc gtacaccccg acgccgacgc
    11821 gccttccgag gatgttgtcc cggtgagggt cgctcttcat gaagcctcgt acggcgccca
    11881 catgctgccg cccgctccca cgttctcccc tgccacgttc cacgacacgg acctgagctt
    11941 ggatcccagc gaagcggtgt ggaggatccg cccggtgtat gccatctgtc ggctgtgctc
    12001 gtccgcgatc gcccggggcc gccagccacg gtccagcggc cccggtggcc gccctgcgga
    12061 ccgacatcgg catcgggtgc cggtcttcag cgaggatctg cctgaacatg accaggaggc
    12121 gctactcagg atgagggagt ggaaccggag ctaccagcgg agcttcagcg cccgggctgc
    12181 gcgagcttcg tcgagccgac gcacgggtgt ggtcacgggc gcctcccgga gcaggtcggg
    12241 ccgctcccga gcctcctccg ccgtccggcg gagcgcctcg ccgaaggcct cgagggtcgc
    12301 cttcgactcc gtctccgtcg gctccaccat catggcctcc tccacgacga gggggaagta
    12361 caccgtcgac gggtggaacc ccaggtcgat catgcgtttc gcgacgtcca tggccctgat
    12421 cccggtcgcg tctcggagcg gtttcgcggt ggccacgaac tcgtgcatcg gccgcatgcc
    12481 cggatgcgcg agcgggaact cctcctccac gaggcgagcg aggtagttcg cgttcagcgc
    12541 cgccagctcg ctcacctccc tgagcccgtc ggcaccatgc aggaacacgt aggcgtaggc
    12601 ccggacaagg atcccgatgt tcccgtggaa cccgtgcatc cgaccgatcg agcgctcccc
    12661 accggcgtcg agcgagaatc ccccatcccc gttcctccgg accgtcggtg cgggcaggaa
    12721 ggcggccagc tcgtccttca cccccaccgg tcccgccccc ggcccacctc ctccgtgtgg
    12781 cgtcgcgaac gtcttgtggg tgttgatatg gacgatgtcg aagcccatgt cgccgggacg
    12841 acaccgcccg aggatcgcgt tcaggttcgc cccgtcgtaa tagaggagcc caccgacacc
    12901 gtggatcgtc gccgcgatct cctcgatctc ctcctcgaac agaccgaggg tgttcgggtt
    12961 ggtgagcatc agaccggcca cggagtcgtc cacgagcttc gccagcgccc cggcgtcgac
    13021 cagaccacgg gcgtcggag
//
LOCUS       DASUYD010000111        26611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_499642, whole genome shotgun sequence.
ACCESSION   DASUYD010000111 DASUYD010000000
VERSION     DASUYD010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 26611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26611
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499642"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF195_05975"
     CDS             <1..474
                     /locus_tag="VF195_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEX6580399.1"
                     /translation="DVLSLIDKATDTLDADEAKASAEKMLQGAFTLEDFLAQLRQMRQ
                     LGPIQDLLKMLPGMPGGKNAIKDLADQVDESKLRQAEALILSMTPDERRNPGIISGSR
                     RLRIARGAGSTTSDVNTLLKDFEGARKMMRTMMGGGKGLPGMFSMPPKVSRGPKR"
     gene            491..1057
                     /gene="rpsP"
                     /locus_tag="VF195_05980"
     CDS             491..1057
                     /gene="rpsP"
                     /locus_tag="VF195_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX6580400.1"
                     /translation="MTTRIRLRRMGSNKRPFFRVVVADQRSPRDGRFIENIGKYHPLD
                     DPSVIEIDQDRALHWLRVGAQPSDQVRNLMRKIGIWDEFVKERPSAAPAPSKERPDKP
                     RISKKAKAKAKAEAAAAQPETPPADEPQAIEEPTKEEPMIAEADAAEAMEAVEEEVPS
                     GDATVDGAVAEDATPTDDTPAAETLEGS"
     gene            1054..1284
                     /locus_tag="VF195_05985"
     CDS             1054..1284
                     /locus_tag="VF195_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX6580401.1"
                     /translation="MKDLVEFLVQELVDEPDAVTVEESFDDRGPRYRVRVAPEDMGKV
                     IGRGGRTAKAIRTVVRAAAQRQGHGAMVDIED"
     gene            1286..1831
                     /gene="rimM"
                     /locus_tag="VF195_05990"
     CDS             1286..1831
                     /gene="rimM"
                     /locus_tag="VF195_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX6580402.1"
                     /translation="MDEPTVVVGIVTGAHGLRGEVSVQNRSDNPDRWTPGGTVFRDGG
                     GPLTISASRRHGRRLLVSFGGVSDRSSAEALRGTVLVVPESWLPDLPEGEWWAHQLEG
                     CVVRTVSGRILGVVREVIPNPANDLWVAVDDAGRETLVPALADLLIDVDVAARTILVD
                     DVPGLTAPDEPPGDEQEDEPG"
     gene            complement(1828..2529)
                     /locus_tag="VF195_05995"
     CDS             complement(1828..2529)
                     /locus_tag="VF195_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX6580403.1"
                     /translation="MRRADDLEIGRIMTGAPFLEEVPHKELDRFAAQGAVHRFRRGTY
                     LCHQGDPDGDVFFLIAGRVEISSETANGNRVLHATLDTPGFLGELSALGKMPRTASVL
                     TLDDSDVWSIGPESFLEFVTAEPTASRGFLQALARQVREHQSFTDDLLHLDLKGRVAK
                     RLLQLVTPTLDDLPTDGVLVPAVTHADLASLCGGSRENVTRILTEWQRRGLIERDGHR
                     YVLKKVAGLAKIADL"
     gene            complement(2650..2796)
                     /locus_tag="VF195_06000"
     CDS             complement(2650..2796)
                     /locus_tag="VF195_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580404.1"
                     /translation="MADKKITPKAASSKDKQAGDRAAARVSLKRTHKQTTKKTAKKLS
                     AKKN"
     gene            complement(2970..3182)
                     /locus_tag="VF195_06005"
     CDS             complement(2970..3182)
                     /locus_tag="VF195_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020512244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2180 family protein"
                     /protein_id="HEX6580405.1"
                     /translation="MDCWHCRRNAVGVCRFCGRGVCEDHAETQPFVLELFRGKDVTRA
                     LVVEDALWCKVCTPRPDPVDLPELDS"
     gene            complement(3194..5473)
                     /locus_tag="VF195_06010"
     CDS             complement(3194..5473)
                     /locus_tag="VF195_06010"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580406.1"
                     /translation="MPTIILFVLFAVGLIIAVDSLRQRRIRATRDIDLRGLVTTRLAE
                     PTAPILATALERALAERPVVRADVYVRLAGALEIADQRPRMRDGCEVKIFRLRWGNDY
                     AMLARDDRELHYRLEVWEAELLPKMDGSRTVGELVVERMEEGGGLDAEPVTDLVLALY
                     SGGFLDPAPVPTDELIADRLDPSSPGRKKLKRFGKTLSIDWKGADRLVRFSYRYVLRP
                     FFWWPVALISGVVAIAGLIAFLDVAIARDFELGSQNAAVESSILLALAFFLTFMHELG
                     HAVVISRYDRKVKSAGFMIYFGAPAFFVEATDSLMLDRRQRILQSSAGPFTELVIAGI
                     AGLAVFLFPGGPLADLLYKFALLNYLVIFLNLIPLLELDGYWILSDLIQVPDLRPRSL
                     QFVQHDLWHKLRARARLTPQEWGLGGYAVAGIAFTIFSFITAFIFWKVIFGELILTLW
                     RGKIGSKILLFLLAAFLAGPVIRGLLNLLRVLLRRIGGLVRAIRFRLETTWRVEAAEL
                     IDELPAFDDLPEDVLSDLAGRVHHRILRAGEPVFRQGDRPDAFYVVRTGSVQIEDEDP
                     ETGDTRVLRTMSRGESFGEMGLLGSHRRQATVRALDEVELFEVDKGTFDRLLAEDMRA
                     PDFGHTMQALAELRELPVFGALDSEQLTVILEHGVWITAAPGEALITQGEAGDRFYAI
                     ASGRAEVVRDGTSVGSLGRGRYFGEVALLRDVPRTATVTASTPMRLFALDRDGFELVV
                     ADAFRRGTLRPAADRIWQH"
     gene            5556..6227
                     /gene="trmD"
                     /locus_tag="VF195_06015"
     CDS             5556..6227
                     /gene="trmD"
                     /locus_tag="VF195_06015"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX6580407.1"
                     /translation="MRIDVFTIFPAILEAPVRESLLGRAIAERLLEVRIHDLRAWSTG
                     RHRSVDDEAYGGGPGMVLKPEPIFAAVESLDPDRGRVLLLSPAGRRLEQSLVRELAGE
                     QHLTLVCGRYEGVDERVVEGLPAEEVSIGDYVLAGGEVPALVVIEAVTRLIPGVIGKE
                     ESHERDSFAEPGMLDHPHFTRPAEFRGMAVPEVLRSGDHGAVERWRREAAREKTVRNR
                     PDLAG"
     gene            6315..6653
                     /gene="rplS"
                     /locus_tag="VF195_06020"
                     /pseudo
     CDS             6315..6653
                     /gene="rplS"
                     /locus_tag="VF195_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237373.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            6913..7647
                     /gene="lepB"
                     /locus_tag="VF195_06025"
     CDS             6913..7647
                     /gene="lepB"
                     /locus_tag="VF195_06025"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6580408.1"
                     /translation="MTETRDPAEVADPAPSEPRVPDGEASTSEPRRTPRAGRHRHEPQ
                     HVTGTLRELVPLVFSALLLAIVIKSFLIQAFFIPSGSMEPALRPGDRILVCRVCKLFS
                     DVTYGDVIVFSDPAPAPHEARGIVGGFVHWLGEGIGVARPEDDDFIKRVVALEGDTWE
                     MRRGTLFVNGARVTEPYVAEEQDRRDFGPETVPDGMLFVLGDNRLHSGDSRFPPPGGL
                     GYIPEEKVIGEAFVIAWPFDRIGGIG"
     gene            7614..8318
                     /locus_tag="VF195_06030"
     CDS             7614..8318
                     /locus_tag="VF195_06030"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEX6580409.1"
                     /translation="MAFRQDRRDRLTVDLIRFERALREQGFSRVAGADEAGRGALAGP
                     LVAAAVVLPDGFDPEGIDDSKVLTRNQREAAYERIVAEAAFAVAKAEPGVIDSRGLHR
                     SNVALLRRCVRALDPPPEYALTDGFPVPRMPCPSLAMKKGDAVAVSVAAASIVAKVTR
                     DRIMVRMHRRYPAFGFDHNVGYGTPEHLEVLDRLGPTPIHRLSFACVGQPLLPGMDRS
                     IGLPTWEPPSHESMTP"
     gene            8315..8623
                     /locus_tag="VF195_06035"
     CDS             8315..8623
                     /locus_tag="VF195_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011981967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HEX6580410.1"
                     /translation="MNEDDIERFEEERELELYREYRDVLPMFAFVIETERRFYLANKV
                     DLEHLDGGWVRLELSDAWVWDMFRPARFVSSVRVVTRHDVNVEELRQDATSIPDAPDV
                     "
     gene            8623..9450
                     /locus_tag="VF195_06040"
     CDS             8623..9450
                     /locus_tag="VF195_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HEX6580411.1"
                     /translation="MPSPEVFAAFAVASLALLIVPGPSVLYIVTRSVDQGKTAGLVSV
                     LGIHTGSIVHVVAAALGLSAILASSALAFGIVKYAGAAYLIWLGIKAIRARGEERTEP
                     RGREHSLARIYAQGVVVNVLNPKTALFFLAFLPQFVDVSKGSVPLQAVIFGALSSGWG
                     SSRTGRTRWFPPASRSRDQGTTHPQAGTLAPRSDLDRSGRRVGAHGPPLRRRVALSAT
                     RASVVAWRRDPSGRGERMRPTTRTVDAAIGSWRATGIAGSASWTSSWSGEGCSSSAR"
     gene            9369..9644
                     /locus_tag="VF195_06045"
     CDS             9369..9644
                     /locus_tag="VF195_06045"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX6580412.1"
                     /translation="MARNWHCRIGELDLIVERRGVLVFCEVKSRRGSAFGVGYEAVTR
                     RKQMKLRSLAEAFLQATGSRPLAIRFDVASVAVRGERSAVELFEDAF"
     gene            complement(9612..11117)
                     /locus_tag="VF195_06050"
     CDS             complement(9612..11117)
                     /locus_tag="VF195_06050"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6580413.1"
                     /translation="MPEPLDHPEPELIDIRDPERSRAALERLGERTWTEALDWLYGRA
                     ADRPTDPDLYPEMRRRFFGERGRPGPAPSQGSRSDEVFSEFRDRLAPFAYAAQHPGSN
                     SYFTPPPLPMSIAGETLSRWLNQGIDLWLAGMAGPFVEEEVVRWLCDLCGFDADAWGV
                     LTSGGVMANLMALTVARDVHLAGLLGGLPRGAALEGIRVYASDQTHFSVERSVDLLGF
                     PEGTLRIIPSDTDFRLPSASVVAAIAEDRAAGLTPLAICAVAGSTNTGSIDDVPALAD
                     LAEREDLWLHVDAAYGAAARLSPREARKLPGLERADSLTVDPHKWLFQPYDLGGLLVK
                     RRDDLLRTFHREPEYYAVWGPEEQPLHWYQYSLEGTRPFRALKLWLSWKHLGSDGFAQ
                     LIERNVDLAQHLARRARELGFEVIEPELAVVCLRLAPPDLDHAQTDELQKRLQRALEV
                     SGIGWLSTTTLRGRTWLRAGVINYLSTDEDTDRVIETLTAASEGILEELDG"
     gene            11191..12729
                     /locus_tag="VF195_06055"
     CDS             11191..12729
                     /locus_tag="VF195_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011056442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEX6580414.1"
                     /translation="MYGRVLGVAVLGVGGHLVTVEAHVGRGLPALIVTGLPGAGVQDA
                     RERVRPAVEGAKLEWPLRRVVVNLAPANVRKEGPGLDLPLVMSVLAATHQIPHDRLRA
                     LAFAGEVSLRGELLATPGILSVAIAAARAGLDGIVVPSANAVEAAQVEGLRVIGAQTL
                     SDAVGFLRGTWSAPEVADAADDAPATATVDLCEVRGQTQARRALEVAAAGGHNLLMVG
                     SPGAGKTMLARRLPTILPELSREESLEATQLHSVAGLLGASRGLLRVRPFRAPHHSVS
                     TVGLLGGGSTSVRPGEVSLAHHGVLFLDELTEFRRDAVEGLRQPLEDGRVVITRAGGS
                     VEFPARFTLVAAANPCPCGFDGDGVRRCVCRPDRIQLYRNKLSGPLLDRIDIRLRIPR
                     LTKEELLGSSSGETSALARTRVVEARDRQRRRWTALGVSCNAHLPGPVARRTCSLSSG
                     AEALLTKAVEMFALTGRGFDRAIKVGRTVADLAGSERIETEHLAEALSYRTGFGTEEL
                     ARAV"
     gene            12719..13867
                     /gene="dprA"
                     /locus_tag="VF195_06060"
     CDS             12719..13867
                     /gene="dprA"
                     /locus_tag="VF195_06060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX6580415.1"
                     /translation="MPSDPQGPPPRGWPPGFGAGEVERRAILALSGLRSLRPLCLHAE
                     AWERGTAAGCLESVRSGRLGSDGDREWLLRIDPGAIATRVMDVGARFVTPADPEYDLR
                     LLDLSDPPVGLFLRGRSITDLPDRVAIVGSRKCSDVGRSVAEDLGRALVSAGLAVVSG
                     AAHGIDAAAHRGALQAGGRTVAVLGSGIDVAYPASSRELLGAIARSGTIVSEYPPGVP
                     AGPRHFPARNRIVVALARAMVVVEGEAKSGSRISVDHALELGRDIFAVPGPVTSPLAE
                     TPLEMIRDGATLIRGADDLLADLGVDRELPPPAPPDLDGSEERVWNALAGRLLADAVA
                     RRAGVSVPDAITTLVRLELRGLVTSSGGRFERRHRTTVQVAGAEPTPP"
     gene            13971..14897
                     /locus_tag="VF195_06065"
     CDS             13971..14897
                     /locus_tag="VF195_06065"
                     /inference="COORDINATES: protein motif:HMM:NF001399.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEX6580416.1"
                     /translation="MPERLPPTESALLSDFEDHLALERRLSPNTIAAYRSDLEQLATF
                     LARNRTSLAGARYAILRRFLAQQHTLGYARATIARRVGAIKTFYRWAVSAERVPVDPS
                     LLLGRPKVVNRLPTVLRPNEAEVLAEAPPVDGEGDPLEHAVALRDRAALELLYGSGLR
                     VGEVASLTVDRIDVPRGRVLVLGKGSKERDVPISEYAADALRDYLEAGRPRMAAEGSR
                     RLFFNRRGKPFSERDIRSMVQRYGATLLPGRRVTPHTLRHSFATHLLEGGADIRAVQE
                     LLGHASVATTQRYTHVSRRRLFDAYERAHPRA"
     gene            14899..15756
                     /gene="whiG"
                     /locus_tag="VF195_06070"
     CDS             14899..15756
                     /gene="whiG"
                     /locus_tag="VF195_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018334817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor WhiG"
                     /protein_id="HEX6580417.1"
                     /translation="MATAKKVATKPAARARKTPAKNARAKTVTIPAVVDDELAIRWDV
                     FKRTADGDAREKLILHYAPLVKYVASRVASGLPSSVDQHDLVSYGMFGLIDALEKFEP
                     GRGNKFETYAIPRIKGAIIDELRAMDWVPRSVRFKARELEKAQADLEAMFKRQPTEEE
                     LADRLGMNRRELHEMVSQISFVSVLALDEVVNAGSDRGESVALIDTLADKGIDPTWGV
                     ESQETRGMLAAAINSLSEREKIVVTLYYFEGLTLAEIGEILGVTESRVCQIHTKAVGG
                     LRGQLSEFD"
     gene            15817..16314
                     /locus_tag="VF195_06075"
     CDS             15817..16314
                     /locus_tag="VF195_06075"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX6580418.1"
                     /translation="MRKLVLWCAVWSAVFVSAVPALARASGDWTWPVVGPVTRGFDPP
                     DSPFGSGHRGIDIAAPVGTPVRAATTGTVTFAGPVGGRLFVTVDHGAGLESTYSFLDS
                     IAVRRGDVVSPGQAIAHSGRGHAGDIVPGLHFGVKLADTYVDPLEYLGPVEVWRFIRL
                     APLES"
     gene            16353..17849
                     /locus_tag="VF195_06080"
     CDS             16353..17849
                     /locus_tag="VF195_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6580419.1"
                     /translation="MRAAHRSRVPRRSTTLAGCTLAVVLGMPLVALAEWPQFQGGPSH
                     DGLSDGPSAPLEVAWRHGDIAIDDADVTGGLSSPVVADNGTIVVVGPHEVLGFDDENG
                     SEVFTAPRDLGPSVTPAIGSGPDGQVVVFAEGFGDRGPATTASPTASSTASPSASPGE
                     EGDTGSHLVAIDLRSGETVWPSSVPLEDVVRTPVTADETAVYVGDFGGHVTAVELSSG
                     ELRWSTDIGSSIAGAVTVDGDRALVATVGEQQTPGAVVALDRSTGEELWRTGEDAVQG
                     NLVSGPVVADGMILVLEPGSVIALDAAEGRFLWRSEIVNPLTTPFSPQGISTPAPVSA
                     DGTVYAVDVTGRAYGLDAETGTVLWDQALNDPSPLTPPVLTVAHLLVPTNSGSLYAID
                     RETGHLVFKIDAGEAFLRGLADAGDVLVAVTGVEDAEIVAFGEDPDGALTDEPSPTTF
                     DLGSLLAGFALGGLPVSIVALALASPLQRRLGPTPRPGSDLDPEEGAG"
     gene            17939..18793
                     /locus_tag="VF195_06085"
     CDS             17939..18793
                     /locus_tag="VF195_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580420.1"
                     /translation="MPPIRTAFARGLAATWSSPLVVGATVAWLLIEWLVVVALGYPGP
                     FALLAHIVAPAPLSTTTDLSVSIGILGVGKGLPLVLIPALVHALLHAIVVGLAVEAVE
                     TGRASRWGAIRGLRALPVAFAIHAFGVAVLFTSQIVAGFGGGGLSFILQLSILVIAVW
                     VFAFAPVIAVTEGRRFLDCLGRALRAARLPGSGNLTFAVLYVVPWFAISVATIVGSVP
                     GSGLGVNPAYTAWVFVLAVSLLHTAIIGAIAIRYLAIADEVPDAPVRAAPERGARSRG
                     ARSGSGRR"
     gene            18958..19890
                     /gene="rpsB"
                     /locus_tag="VF195_06090"
     CDS             18958..19890
                     /gene="rpsB"
                     /locus_tag="VF195_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017540076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX6580421.1"
                     /translation="MPVVTRRELLEAGVHFGHQTRRWNPKMQRYLFGERSGIYIIDLE
                     KSLAGIEETFEFVRDLGRRRGTVLFIGTKKQAQEVVEEHAGRVGMPFVNNRWLGGMLT
                     NFSTISKRLVRLRELREMESSDAMDNLPKKEVIRLRREREKLERNLGGIQALERLPDA
                     VFVVDTKKEQIAVTEARKLEIPVIAIVDTNCDPDEVDYVIPGNDDAIRAVSLVARVVA
                     DALAEGYGMAKDEVVEKSTARRPTAAPRRSTTAAPAPADEDVPSAEDAAAIAASTVFE
                     PDLPTPETAEEEPEVVAEPAIDEPASAAPSRGEA"
     gene            19887..20498
                     /gene="tsf"
                     /locus_tag="VF195_06095"
     CDS             19887..20498
                     /gene="tsf"
                     /locus_tag="VF195_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013682496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX6580422.1"
                     /translation="MIAISADQVKMLRERTGAGMMDCKRALQETDGDLERAIELLRER
                     GLASVAKRADRTADQGVVDSYIHFNNTVGVLVEVNSETDFVAKTDEFRQLVKDIALHI
                     ASPAAPRWLTREDVPADVIRAEEHIALAQAKEAGKPENVLDRIVEGKLNAFYQGSVLL
                     EQPYVKDDAKTIQQLLDEVAAKVGEKVAVRRFVRYKLGESTEA"
     gene            20498..21286
                     /gene="pyrH"
                     /locus_tag="VF195_06100"
     CDS             20498..21286
                     /gene="pyrH"
                     /locus_tag="VF195_06100"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX6580423.1"
                     /translation="MALDADVAGTITRGAHANVTERSPGGTGYHRVLLKLSGEAFAPT
                     DGMGISTEATALIGRQLAEVVDLGAQVAVVVGGGNIWRGRQAPAIERSRADYMGMLAT
                     VMNSLALQDSLEKLHVTTRVQTAIQMTQLAEPFIPLRARRHLEKGRVVIFAAGLGVPY
                     FSTDTCAAQRALEIGANALLKGTKVDGVYTADPKLVPGASRLAEVGYVDILREGYQVM
                     DATAISLCMENDLPIIVFDLWEEGNIRRVVMGETVGSLVHGGTR"
     gene            21283..21843
                     /gene="frr"
                     /locus_tag="VF195_06105"
     CDS             21283..21843
                     /gene="frr"
                     /locus_tag="VF195_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013223865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX6580424.1"
                     /translation="MTIDSSLKEADHKMEQALAHLREDLSSVRTGRASGSVLSRITVE
                     YYGTPVPLNQLASVNVPEPRLLVVQPFDKNSIAQIEKAIQQSDLGITPSNDGQVIRLA
                     FPPLTEERRKDLVKQVHHRAEEGRVAVRNVRRHAKDDMEKLEREHSISEDELSRGEKE
                     LQKLTDRYVASIDEVQRHKESELMEV"
     gene            21843..23429
                     /locus_tag="VF195_06110"
     CDS             21843..23429
                     /locus_tag="VF195_06110"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX6580425.1"
                     /translation="MPDDERESDDLFEDLDKFFAPIKDVDWDEREGAPATTASPGPSV
                     SEEEHVTVRSEPEQVVSVPAADDETPATVFDDDDDDDDDGDWYDTASLEPVGQALGGS
                     VGNDDDEDEMPPGGPAVMQDADQDETDLFAPPAAADDDWPADEVEDQQAARPSDAELE
                     EAAAHFAESLGDEGTAAAPRVVQIDEENATDLMAELGAEPYPPDEQEDVFAAMAEEEP
                     SRTIVVGGEGLGGPSWQEPAAVEVGADLDRRAAERDMPAAFLTGIVLAGVAVVALLIG
                     EGVFAIVAAVVALVAQGELYGVMVKHHRQPATAVGLVSGALVLAGAYYHGEAGLTSMV
                     SLGLLATFLWYLTVPAPHRKDLVGNIGLTLLGLVWIPALAGSLLIILTTFGAEVVVAV
                     IALTFVFDTAAFLIGSVWGGSFVQRPLAPATSPKKSIEGLVGASLITVIVSMAFITSF
                     VDVAPEIFSGARVNAALLGIVVSLAATFGDLAESLIKRDVGVKDMGSVLPGHGGVLDR
                     IDSLLFVAPAALLLLRVIAF"
     gene            23503..24660
                     /gene="dxr"
                     /locus_tag="VF195_06115"
     CDS             23503..24660
                     /gene="dxr"
                     /locus_tag="VF195_06115"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012867338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEX6580426.1"
                     /translation="MRSVTILGSTGSVGTQALDVVRRNPERFKVIGLSAAGTNQELLA
                     GQIREFLPPVVGIADEQAAEEVRTHLGSIRGVDFIVGPDAAERLASEAEADIVLNALV
                     GSSGLGPTLAALQSGKTLALANKESLVVGGELVMDLIKGEPERLLPVDSEHAALAMAL
                     RGERREDLKRVVLTGSGGPFRGWTATELAKASMKEALQHPVWSMGPKITIDSATLMNK
                     GLEVIEAHYLFGLEYSQIHVLIHPEGLVHAIAEFRDGSMRAEMATADMRLPIQLALAW
                     PERLPTGVASLPLTESPFTFEPVDHEAFPAVDLAYRVGGLGLTFPAVMNAANEVAVMA
                     FLEGKIPLPRIVEIVQTIVDEHEPPSVVSVVHIERADTWARRRTVEIIEAR"
     gene            24661..25785
                     /locus_tag="VF195_06120"
     CDS             24661..25785
                     /locus_tag="VF195_06120"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX6580427.1"
                     /translation="MGGPLAIVGFLALILLVVIPIHELGHLLVARRFGFKIQEYFVGF
                     GPKLWSMRRGETEYGIKAIPAGGYVKIAGMNPYETVPAQDVARAYGSKPIWQRALVIL
                     AGPLSHALVGALLYFTLFVTYGDPNTELVVVGEVAPTLDGSSSPALEAGLQVGDVVTR
                     VGDLEGPTPDSLGRYVTRFAEDRPGEPLTYVIERDGRHLTLEIVPRLVTEDEIARGRI
                     GFTLGAEDLSIPGSVVESGEWVGRAAWNSVTTIPKVFTVGVGRTFSVLFTDEPRRDDD
                     VTSLVGVSRQIGGAGERGDWGLFLNFVAYVTIFIGVVNLIPLPPLDGGHLLLLAWEKV
                     TGRQADYRKLVPVSAAVIVFLSVFAIATVFLDITEPLPLI"
     gene            25892..26581
                     /locus_tag="VF195_06125"
     CDS             25892..26581
                     /locus_tag="VF195_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580428.1"
                     /translation="MAAQTVLVVEDEDAIAEAVRARLRSEGYHVVVASDGPEALRRHA
                     ETRPDLVVLDLMLPGMDGLEVCTRIQRNGWTPVLMLTAKTEEADKVAGFAVGADDYLT
                     KPFSLRELSVRVQAILRRRERIETSSNGEPIEHEGLMIDAKRRRVEVDGSEIPLTPLE
                     FEILLTLAREPGVVLSRDQLMDRIWGYRDHAGGRVVDSHVARLRRKIGEDGNDPRFIR
                     TVHGVGYAFKE"
BASE COUNT         4036 a         8901 c         9523 g         4151 t
ORIGIN      
        1 gacgtgctca gcctgatcga caaggccacg gacaccctgg acgccgacga ggcgaaggca
       61 tccgccgaga agatgctcca gggagcgttc accctcgagg acttcctcgc gcagctccgg
      121 cagatgcggc agctcggacc gatccaggac ctgctcaaga tgctcccggg catgccagga
      181 ggcaagaacg ccatcaagga ccttgcggac caggtggacg agagcaagct ccggcaggcg
      241 gaggccctga tcctgtccat gacgccggac gagcgacgga atcccggtat catctccggg
      301 tctcgccgcc tgcggatcgc ccgcggggca ggctccacca cctccgacgt caacacactc
      361 ctcaaggact tcgagggcgc gaggaagatg atgcggacga tgatgggcgg ggggaagggc
      421 cttccgggca tgttctcgat gccgcccaag gtatcgagag ggccgaagcg ctagcgagag
      481 gaacgcggag atgacgacca ggatccggct gcggaggatg ggttcgaaca agcgcccgtt
      541 cttccgcgtc gtggtggccg atcagcggag cccccgcgat ggccgcttca tcgagaacat
      601 cggcaagtac cacccgctcg acgatccgag cgtgatcgag atcgaccagg atcgcgcgct
      661 gcactggctg cgcgtcggcg cccagccgtc cgatcaggtc cgcaacctga tgcggaagat
      721 cgggatctgg gacgagttcg tgaaggagcg cccgtccgcg gcgccggcgc cgtcgaagga
      781 acgaccggac aagccgagga tctccaagaa ggcgaaggcg aaggcgaagg cggaggccgc
      841 cgcggcgcaa cccgagacgc ctccggccga cgagccccag gcgatcgagg agcccacgaa
      901 agaggagccc atgatcgcgg aggccgacgc cgccgaggcc atggaggccg tggaggaaga
      961 agtgccctcc ggggacgcga ccgtcgatgg cgcagtcgcc gaggacgcga cgcccacgga
     1021 cgacacaccc gccgccgaga cgctcgaggg atcgtgaagg acctcgtcga gttcctcgtc
     1081 caggagttgg tcgatgaacc cgacgccgtc accgtcgagg agtcgttcga tgaccgggga
     1141 cctcgttacc gggttcgcgt cgccccggaa gacatgggca aggtgatcgg ccgcggaggc
     1201 cgcacggcca aggcgatccg caccgtcgtc cgcgcggccg cgcagcgcca gggacacggt
     1261 gcgatggtgg acatcgaaga ctgaggtgga cgagccgacc gtcgtcgtcg gcatcgtgac
     1321 gggcgctcac ggtctccgcg gcgaggtttc cgttcagaac cgttccgaca accccgatcg
     1381 atggacgccg gggggcaccg ttttccgtga tggcggcggt ccgctcacga tctccgcgag
     1441 ccgccgccat gggcgccggc tgctggtgag cttcggcggg gtctccgacc ggagcagcgc
     1501 cgaggccctc cggggaaccg ttctcgtggt gccggagagc tggctccccg acctccccga
     1561 aggcgagtgg tgggcccatc agctcgaagg gtgcgtggtc cggacagtgt ccggccggat
     1621 cctgggagtg gtcagagagg tgatcccgaa tccggcgaac gacctgtggg tcgcggtcga
     1681 cgacgcaggt cgggagacgc tcgtgcccgc cctcgcggac ctcctgatcg acgtcgacgt
     1741 ggcggcccgc acgatcctgg tcgatgacgt gcccgggctc accgcgcccg atgagccgcc
     1801 aggagacgag caggaggacg agccgggcta gaggtcggcg atcttcgcca atccggccac
     1861 cttcttgagc acgtaccggt ggccgtcgcg ctcgatcagg ccgcgtcgct gccactccgt
     1921 gaggatgcgg gtcacgttct cgcggctgcc tccgcacagg ctggcgagat cggcgtgggt
     1981 gaccgccggg accagcacgc cgtcggtcgg gaggtcgtcc agggtgggcg tcacgagctg
     2041 gaggagccgt ttggcgacgc gtcccttgag gtcgagatgg agcaggtcgt cggtgaacga
     2101 ctggtgttca cggacctggc gggccaacgc ctggaggaac ccgcgggagg cggtcggctc
     2161 cgcggtcacg aactcgagga acgactccgg gcctatggac cagacatccg agtcgtcgag
     2221 ggtgaggacg ctcgccgtcc gcggcatctt cccgagcgcc gacagctcgc cgaggaagcc
     2281 gggtgtgtcg agggtcgcgt gcaggacccg gttcccgttc gccgtctcgg agctgatctc
     2341 gacgcgaccg gcgatcagga agaagacgtc accgtcgggg tcgccctggt ggcacagata
     2401 cgtgcctcgg cggaagcgat ggacggcgcc ctgcgcggcg aatcggtcga gctccttgtg
     2461 gggaacctcc tccaggaagg gcgcgccggt catgatcctg ccgatctcga ggtcgtcggc
     2521 tcgccgcatg gaacggagcc taccggggcg tcgacgggca cgcgacccct gacgcggagg
     2581 gacgcgaaag ccgacgcgaa aagggcgggg gccgaaggcc cccgcccttg ttcgcagatg
     2641 cgtggaggat cagttcttct tcgcgctcag cttcttcgcg gtcttcttgg tggtctgctt
     2701 atgcgtccgc ttgagcgaga cgcgagcggc ggcgcggtcg ccggcctgct tgtccttcga
     2761 agacgctgcc ttcggcgtga tcttcttgtc tgccatcccg gatcctcctt ctgtgcacgt
     2821 gggcccctct ggcccttcga cacccgaagc ctgtcacccc ggtcacggat cgacggtgcg
     2881 cccggtcacg ttcctcgcgt gacgttcgtc acgccgtccg gtgtgacgtg cacccgacct
     2941 cgggtgtgac gtacgtcaca acacctggct caactatcca gctccggcag gtcgaccggg
     3001 tcgggccggg gcgtgcagac cttgcaccag agcgcgtcct cgacgacgag ggcgcgcgtc
     3061 acgtccttcc ccctgaagag ctcgagcacg aacggctggg tctccgcgtg gtcctcgcac
     3121 accccgcgtc cgcagaagcg acagaccccc acggcgttcc tgcggcagtg ccagcaatcc
     3181 acgcgatccc tcctcagtgc tgccagatgc ggtcggcggc ggggcggagc gtaccgcgtc
     3241 ggaacgcgtc ggcgacgacg agctcgaacc cgtcgcgatc gagggcgaac aggcgcatcg
     3301 gggtcgaggc ggtgacggtc gcggtccgag ggacgtcgcg gagcagcgcc acctcgccga
     3361 agtaccggcc cctccccagc gaccccacgg acgttccatc gcgcacgacc tccgctcgcc
     3421 ccgacgcgat cgcgtagaac cggtcgcccg cctctccctg cgtgatgagg gcctccccgg
     3481 gggcggcggt gatccatacg ccgtgctcga ggatcaccgt gagctgctcc gagtcgaggg
     3541 caccgaagac aggaagctcg cgcagctccg ccagggcctg catcgtgtgg ccgaagtccg
     3601 gcgcgcgcat gtcctcggcg agcagccgat cgaacgtccc cttgtccacc tcgaagagct
     3661 cgacctcgtc cagggcgcga acggtggcct ggcgacgatg ggagccgagg agccccatct
     3721 cgccgaacga ctcgcccctc gacatcgtcc tgaggacgcg ggtgtcgccg gtctccgggt
     3781 cctcgtcctc gatctgcacg gaccccgtgc ggacgacata gaacgcatcc ggacggtctc
     3841 cctgacggaa caccggctcg ccggcgcgga ggatcctgtg gtggacccgg cccgccagat
     3901 ctgacagcac gtcctccggc aggtcgtcga acgccgggag ctcgtcgatc agctccgcgg
     3961 cctcgacccg ccaggtggtc tcgagtcgga accggatcgc ccggaccagg cctccgatcc
     4021 ttcgtagcag gacccggagg aggttgagca gccctcggat caccggaccg gcgaggaagg
     4081 ctgccaagag gaagagcagg atcttcgagc cgatcttgcc ccgccagaga gtgaggatca
     4141 gctcaccgaa gatcaccttc cagaagatga acgcggtgat gaaggagaag atcgtgaacg
     4201 cgatcccggc gaccgcgtag ccgccgagac cccactcctg cggcgtgagc cgcgcgcgcg
     4261 cccggagctt gtgccagagg tcgtgttgca cgaattggag cgagcgcgga cggaggtcgg
     4321 ggacctggat caggtccgag aggatccagt acccgtcgag ctccagcaac gggatcaggt
     4381 tcaggaagat cacgaggtag ttgaggagcg cgaacttgta gaggaggtcc gcgagcggcc
     4441 cgccggggaa gaggaagacg gcgaggccgg cgatgccggc gatcacgagc tcggtgaacg
     4501 gtccggccga ggactgcagg atgcgctgcc gccggtcgag catcaggctg tccgtcgcct
     4561 ccacgaagaa cgccggcgcg ccgaagtaga tcatgaagcc cgcgctcttc accttgcggt
     4621 cgtagcgcga gatcacgacg gcgtgcccga gctcgtgcat gaacgtcagg aagaacgcca
     4681 gggcgagcag gatcgacgat tcgactgccg cattctgact cccgagttcg aaatcccgcg
     4741 cgatggcgac gtccaggaac gcgatcagac ccgcgatcgc gacgaccccc gagatcaggg
     4801 cgacgggcca ccagaagaac ggacgcagga cgtaccggta gctgaagcga accagccggt
     4861 cggcgccttt ccagtcgatc gagagggtct tgccgaagcg cttgagtttc ttccgccccg
     4921 gcgacgaagg atccagcctg tcggcgatca gctcatcggt ggggaccggg gcgggatcga
     4981 ggaaccctcc cgagtacagc gcgagcacca ggtcggtgac gggttccgcg tcgaggccgc
     5041 cgccctcctc catccgttcc accacgagct cacccaccgt cctcgagccg tccatcttcg
     5101 gcaggagctc cgcctcccac acctccaggc ggtagtggag ctcgcggtcg tccctcgcga
     5161 gcatcgcgta gtcattgccc catcggagtc ggaagatctt cacctcgcac ccgtccctca
     5221 tccgagggcg ctgatcggcg atctccaggg caccggcgag acggacgtag acgtccgccc
     5281 gcaccaccgg acgctcggcg agcgcccgct cgagggcggt cgcgaggatc ggggcggtcg
     5341 gctccgcgag ccgggtggtc acgagccccc gtaggtcgat gtcgcgcgtg gcccggatcc
     5401 gtcgctgccg gaggctgtcg acggcgatga tcagcccgac cgcgaagagc acgaacagga
     5461 tgatcgtcgg catcgatgcc ctccctccgg ccggggccgg tcggcggcat cctgcctcgc
     5521 gcggcgtgcg gacgcaacgt tgctagcctc gcggggtgcg gatcgacgtg ttcacgatct
     5581 tccccgcgat cctggaagcg cctgtgcggg agagtctcct gggtcgcgcg atcgcggagc
     5641 ggttgctcga ggtacggatc cacgatctgc gggcctggtc gacgggtcgt catcggagcg
     5701 tcgacgacga ggcgtacgga ggcggccccg ggatggtcct gaagccggag ccgatcttcg
     5761 cggccgtgga atcgctcgat cccgatcgcg ggcgggtgtt gctgctctcg cccgccggcc
     5821 gacgcctcga gcagtcgctc gtgcgagagc tcgcggggga gcagcacctg acgcttgtct
     5881 gcggtcgcta cgaaggcgtg gatgagcgcg tcgtcgaggg tcttcccgcc gaggaggtat
     5941 cgatcggcga ctacgtgctc gccggggggg aggtcccggc cctggtggtg atcgaggccg
     6001 tcacacgcct gatcccgggt gtgatcggca aggaggagtc gcacgagcgg gactcgttcg
     6061 cggagccggg gatgctcgac caccctcact tcacgcgccc cgcggagttc cgcgggatgg
     6121 ccgtccccga ggtcctgcga tcaggcgacc acggggccgt ggaacgttgg cgccgggagg
     6181 cggcacggga gaagacggtc cgcaaccgcc ccgaccttgc ggggtagctc gcgaaggacg
     6241 gtagctcaga cgctcgctcg cggagacggt tgggcaggcc ggaggaggct cgctggtatc
     6301 cttgctcgcc cgccatgaac accaccgatc tcgtcgagca gtcttacctg cgcagggggc
     6361 ttccccagtt ccgaccgggt gacaccgtca aggtgcatgc ccgcgtcgtg gaaggcaacc
     6421 gggagcgtgt gcaggtcttc cagggcgtgg tgatccggcg ctcgggcggt ggcctccgcg
     6481 agacgttcac cgtccgcaag atctccttcg gcgtgggtgt ggagcgcacg ttcccgctcc
     6541 actcgccttc gatcgccaag ctcgaggtcg tgcagcacgg gcacgtccgc cgcgcgaagc
     6601 tctattacct ccgcgatctc cgcggcaaga aggcccggat caaggagcgc aggatcgacg
     6661 aggcgaagct cgccgcgatg gaggcgatga gagccgaaga ggaggcgatg ggtgccgccg
     6721 ccgaggaggg ctccatggag gcactcgagg aagcggagac cgcggccgag ctcgagccct
     6781 ccgccggagg cgaggccccc ctggacgggg cgggccccgc ctcggaggag gcggacgagg
     6841 tgggggccgc gtccgaggac ggagccgacc catccgccga cgaggcgggg gagcgggccg
     6901 aggcctgagg gagtgaccga gacgcgggat ccggccgagg tggccgaccc ggcgccgtcc
     6961 gagccacggg tccctgacgg ggaggcgagc acctccgaac cgcgccggac cccccgcgcg
     7021 ggtcgccacc gacacgaacc gcagcacgtc accgggaccc tccgcgagct ggttccgctg
     7081 gtcttctcgg cgctcctgct cgcgatcgtg atcaagagct tcctgatcca ggcgttcttc
     7141 atcccctccg gctcgatgga gcccgccctg cgaccgggcg atcggatcct cgtctgccgg
     7201 gtgtgcaagc tgttctccga cgtgacctac ggcgacgtga tcgtgttctc cgaccctgct
     7261 cctgccccgc acgaggctcg ggggatcgtg gggggcttcg tccattggtt gggggagggg
     7321 atcggcgtcg cgaggcccga ggacgacgac ttcatcaagc gcgtcgtcgc cctcgagggg
     7381 gacacctggg agatgcgcag gggcacgctg ttcgtgaacg gcgcgcgggt cacggaacca
     7441 tacgtggccg aggagcagga tcgccgggac ttcggtccgg agacggttcc ggatggcatg
     7501 ctgttcgtgc tgggcgacaa ccggttgcat tcgggcgact cgaggttccc cccgcccgga
     7561 gggctcggct acatcccgga ggagaaggtg atcggcgagg cgttcgtgat cgcatggcct
     7621 ttcgacagga tcggagggat cggctgaccg tcgacctcat ccgcttcgag cgcgcgctgc
     7681 gcgaacaggg cttctcccgg gtcgcaggcg ccgatgaggc cggccgcggc gcgctcgcgg
     7741 ggccgctggt ggccgccgcc gtggtgctcc ccgacgggtt cgaccccgag gggatcgacg
     7801 actcgaaggt cctcacccgg aaccagcgcg aggccgcgta cgagcggatc gtcgcggagg
     7861 cggcgttcgc cgtcgcgaag gccgagcccg gcgtgatcga cagccgcggt ctccatcgtt
     7921 cgaacgtggc gctcctgcgg cgatgcgtcc gcgcgctcga tccaccgccc gagtacgcgc
     7981 tcaccgacgg gttcccggtg ccccgtatgc cgtgcccctc cctcgccatg aagaagggcg
     8041 atgcggtggc ggtctcggtc gccgcggcct cgatcgtcgc gaaggtgacc cgggatcgga
     8101 tcatggtccg catgcaccgg cgctatccgg cgttcgggtt cgatcacaac gtgggctacg
     8161 gtacgccgga gcacctcgag gtgctcgacc gtctcggccc cacgccgatc caccggctgt
     8221 cgttcgcgtg cgtcgggcag ccgctgctcc ccgggatgga tcggtcgatc ggcctcccga
     8281 cgtgggaacc gccctcgcat gagagcatga ccccatgaac gaggacgaca tcgagcgctt
     8341 cgaggaggag cgggagctcg aactctaccg tgagtaccgc gacgtcctcc cgatgttcgc
     8401 gttcgtgatc gagacggagc ggcgcttcta tctcgcgaac aaggtggatc tcgagcacct
     8461 cgacggcggt tgggtgcgcc tcgagttgag cgatgcatgg gtctgggaca tgttccgccc
     8521 ggcccggttc gtctcatccg tgcgtgtggt gacgcggcac gacgtcaatg tcgaggagct
     8581 ccggcaggac gccacctcga tccccgacgc gcccgacgtg tgatgccctc cccggaggtg
     8641 ttcgcggcct tcgcggtggc gtcgctcgcg ctgttgatcg tgcccggccc atcggtcctc
     8701 tacatcgtca cgcgctcggt ggaccaaggg aagacggcgg gattggtctc tgtcctgggg
     8761 atccacacgg gctcgatcgt ccacgtcgtc gcggccgcgc tcgggttgtc cgcgatcctg
     8821 gcgtcgtcgg ccctcgcgtt cgggatcgtg aagtacgcgg gcgccgccta tctgatctgg
     8881 ctcgggatca aggcgatccg cgccaggggt gaggagcgga ccgaaccgcg gggacgcgag
     8941 cattccttgg ctcggatcta cgcgcagggg gtcgtggtga acgtcttgaa cccgaagacg
     9001 gctttgttct tcctcgcgtt cctgccgcag ttcgtggacg tgtcgaaggg ttccgtgccg
     9061 ctgcaggcgg tgatcttcgg agccctttca tcgggctggg gttcatctcg gacgggacgt
     9121 acgcgctggt ttccgcccgc gtctcgctcg cgcgatcagg gcacgacgca tccgcaggcc
     9181 ggcacgctgg ctccccggtc tgaccttgat cggtctgggc gtcgcgtcgg cgctcacggg
     9241 ccgccgctcc gtcgtcgcgt agccctgtcg gcgacgagag ctagcgtcgt ggcgtggcga
     9301 cgggacccat cgggccgagg ggagaggatg cggccgacta cgcgtaccgt cgacgcggct
     9361 atcggatcgt ggcgcgcaac tggcattgcc ggatcggcga gctggacctc atcgtggagc
     9421 ggcgaggggt gctcgtcttc tgcgaggtga agtcccgtcg cggcagcgcc ttcggggtcg
     9481 gttacgaggc ggtcacgcgg cgcaaacaga tgaagctccg gtcgctggcg gaggcgttcc
     9541 tccaggcaac cggctcccgg cctctggcga tccggttcga cgtggcgagc gtggccgttc
     9601 gaggggagcg ttcagccgtc gagctcttcg aggatgcctt ctgaggcggc cgtcagggtc
     9661 tcgatcacac ggtcggtgtc ctcatcggtc gacaggtagt tgatcacgcc ggcgcggagc
     9721 catgtacggc cacggagcgt cgtcgtcgag agccagccga tgcccgagac ctcgagcgcc
     9781 cgctgcagcc gcttctggag ctcatccgtc tgggcatggt ccaggtcggg cggcgcgagc
     9841 cgcaggcaga cgacggcgag ctcgggctcg atcacctcga agccgagctc ccgagcccgg
     9901 cgcgcgagat gctgtgccag atcgacgttg cgctcgatca gctgcgcgaa cccgtcggac
     9961 cccaggtgct tccacgacag ccagagcttg agagctcgga acggccgcgt cccctcgagc
    10021 gagtactggt accaatgcag cggctgttcc tccggccccc agaccgcgta atactcgggt
    10081 tcgcggtgga acgtgcggag gagatcgtcg cgccgcttca cgagcagccc gccgaggtcg
    10141 taaggctgga acagccactt gtggggatcg acggtgagcg agtctgctcg ctccagcccc
    10201 ggaagcttgc gggcttcgcg tggcgaaaga cgggcggcgg cgccgtacgc ggcgtcgacg
    10261 tgcagccaga ggtcttcccg ttccgcgaga tctgcgaggg cggggacgtc gtcgatcgag
    10321 cccgtgttgg tcgagccggc gaccgcgcag atcgcgagcg gcgtcaggcc ggcggcgcgg
    10381 tcctcggcga tcgcggcgac gaccgatgcg gacggcagtc ggaagtcggt gtcggacggg
    10441 atgatccgca gcgtgccctc cgggaagccg agcagatcga cggagcgttc gaccgagaag
    10501 tgcgtctggt cggaggcgta gacgcggatc ccttccagcg cggcgccccg gggaagtccg
    10561 ccgaggagcc ccgcgaggtg gacgtcccgg gcgaccgtga gcgccatgag gttcgccatc
    10621 acgccgccgc tcgtgaggac gccccaggca tcggcgtcga aaccgcacag atcgcagagc
    10681 cagcggacga cctcctcctc cacgaagggg cccgccatcc ctgcgagcca caggtcgatg
    10741 ccctggttca gccagcggga aagcgtctcg cccgcgatcg acatcgggag cggcggcggg
    10801 gtgaagtagc tgttcgagcc gggatgctgc gccgcgtagg cgaacggcgc gaggcggtcg
    10861 cggaactcgg agaagacctc gtccgacctc gagccctgtg acggcgcggg ccccggccgg
    10921 ccgcgttccc cgaagaacct gcgccgcatc tccgggtaca ggtcggggtc ggtggggcga
    10981 tccgctgcgc gtccgtagag ccagtcgagc gcctccgtcc aggtccgttc gcccagtcgc
    11041 tcgagcgctg cccgcgatcg ctcgggatca cggatgtcga tcagttccgg ttccgggtgg
    11101 tcgagtggct cgggcatccg gcggggatgc tagcgcgagg actcggggtg aacgcctcgt
    11161 gtcggtgggt gggggtatcg tccggcgccc atgtacgggc gggtcctggg ggtggcggtg
    11221 ctcggcgtcg gggggcacct cgtgaccgtg gaagcgcacg tcggtcgcgg acttccggcg
    11281 ctcatcgtca cgggccttcc cggtgcaggc gtccaggacg ctcgcgaacg ggtccgtccc
    11341 gccgtggagg gtgccaagct cgagtggccc ctgcggcgcg tcgtcgtgaa cctcgcaccg
    11401 gcgaacgtcc ggaaggaggg gcccggactg gacctcccgc tcgtgatgag cgtgctggcg
    11461 gcgacccatc agatccccca cgatcgtttg cgggcgttgg cgttcgcggg cgaggtctct
    11521 ttgaggggcg agctgctcgc cactcccggc atcctcagcg tcgcgatcgc ggccgcgagg
    11581 gcggggctgg atgggatcgt ggtgccgagc gcgaacgcgg tggaggccgc gcaggtcgag
    11641 ggtctccgag tgatcggcgc gcagaccctg tccgacgccg tggggttcct ccgtggcacg
    11701 tggtcggcgc ccgaagtggc cgatgctgcc gacgacgcgc cggccaccgc gaccgtcgac
    11761 ctctgcgagg tgcgcggtca gacgcaggcg cgccgggccc tggaggtcgc ggctgccggc
    11821 ggccacaacc tgctgatggt ggggtcgccg ggcgccggga agacgatgct cgcgcgccgg
    11881 ctgccgacga tcctgccgga gctgtcgcgg gaggaatccc tggaggcgac ccagctgcat
    11941 tcggtcgcag ggctcctggg cgcgtcacgc ggactcctgc gggtccgccc gttccgggcg
    12001 ccgcaccatt cggtctccac ggtggggttg ctcggaggag gcagcacctc cgtgcgtccc
    12061 ggcgaggtga gcctcgcgca tcacggcgtg ctgttcctcg acgaactcac cgagttccgc
    12121 cgcgacgccg tcgaaggcct ccgccagccg ctcgaggacg gccgcgtcgt gatcacgcga
    12181 gcgggcggtt cggtggagtt cccggcgcgg ttcacgctcg tcgccgccgc gaacccctgc
    12241 ccatgcgggt tcgatggcga cggcgtgcgg cgatgcgtgt gccgacctga ccgcatccag
    12301 ctgtatcgca acaagctctc aggcccgctg ctcgatcgga tcgacatccg cctccgtatc
    12361 ccccggctga ccaaggagga gttgctcggg tcatcgtcgg gggagacctc ggctctcgct
    12421 cggacgcgcg tcgtggaagc ccgcgatcgt cagcgccgcc gatggaccgc tctcggcgtg
    12481 tcgtgcaacg ctcacctgcc gggacccgtc gcgagaagga cctgctcgtt gtcgtccggc
    12541 gccgaggcgc tcctcacgaa ggccgtggag atgttcgccc tcacgggtcg aggattcgat
    12601 cgggcgatca aggtcggtcg gacggtcgcc gacctggccg ggtccgagcg gatcgagacg
    12661 gagcatctgg ccgaggcgtt gtcgtaccgc accgggttcg ggacggagga gctggcgcgt
    12721 gccgtctgat ccgcagggac cgccgcctcg cggctggcca cccgggttcg gagcggggga
    12781 ggtcgaacgt cgcgcgatcc tggcgctctc cgggctccgc agtctccggc cgctctgcct
    12841 ccatgcggaa gcatgggagc gcggcacggc ggcaggatgc ctcgagtccg tgcgctccgg
    12901 caggctcggg agcgacggcg accgggagtg gctcctccgg atcgaccccg gcgcgatcgc
    12961 tacgcgcgtg atggatgtcg gggctcggtt cgtcacgccg gcagatcccg agtacgacct
    13021 ccggctcctc gacctctcgg accctccggt gggtctgttc ctccgggggc ggtcgatcac
    13081 ggaccttccc gaccgcgtcg cgatcgtcgg ctcgaggaag tgctcggatg tcggccgctc
    13141 cgtcgcggag gacctggggc gcgctctcgt atcggcgggg ctcgcggtcg tctccggcgc
    13201 tgcgcacggg atcgacgccg ccgcccatcg tggcgcgctc caggccgggg gccgcacggt
    13261 cgcggtcctg ggctcgggca tcgacgtcgc ctatcccgcg agcagccggg aactcctcgg
    13321 cgcgatcgcc cggagcggca cgatcgtgag tgaataccct cccggcgtcc cggccggtcc
    13381 ccggcacttc ccggcgcgga accggatcgt ggtcgcgctc gcgagagcga tggtggtggt
    13441 ggagggcgag gccaagagcg gctcgcggat ctcggtcgat catgcgctcg aattgggacg
    13501 ggacatcttc gccgtgccgg ggcccgtcac gagccccctc gcagagaccc cgctcgagat
    13561 gatccgggac ggtgccacgc tgatccgcgg cgccgacgac ctcctggccg atctgggcgt
    13621 cgatcgggaa ctgccgcctc ccgcgccacc cgacctcgat ggctccgagg aacgcgtctg
    13681 gaacgcgctc gccggtcgcc tgctcgccga cgccgtcgct cgccgggccg gggtgtccgt
    13741 gccggatgcg atcacgaccc tcgtccggct cgagctgcgg gggctggtga cgagctctgg
    13801 cggccgattc gagcgacgcc atcgaaccac cgtgcaggtc gcaggggcgg aaccgacccc
    13861 cccttgaccg tgggcccgac ggccgaccgg gtgaccgacg caccccccgt gtgggggctt
    13921 gcgtcgcacc cttccggttg atagacaggg cgacgacccc gggagcgtgc atgcccgaaa
    13981 gacttccccc aaccgaatcc gccctactca gcgacttcga ggaccatctg gccctcgagc
    14041 gtcggctgtc tccgaacacg atcgcggcct acaggagcga tctggagcag ctggccacgt
    14101 tcctcgcccg gaaccgcacc tccctggccg gcgcccggta cgcgatcctg cgccgcttcc
    14161 tcgcgcaaca gcacacgctc gggtacgcgc gggcgacgat cgcgcgccgc gtcggagcga
    14221 tcaagacgtt ctatcgatgg gcggtctcgg cggaacgggt cccggtcgat ccgtcccttc
    14281 tcctcggccg cccgaaggtc gtgaatcgct tgccaaccgt cctccggccg aacgaggccg
    14341 aggtgctcgc ggaggcgccg ccggtcgacg gcgagggaga tccgctggag cacgccgtgg
    14401 ccctccggga tcgggccgcc ctcgagctcc tctacggctc cggtctgcgg gtgggagagg
    14461 tcgcgagcct caccgtcgac cggatcgacg tacctcgggg ccgcgtgctg gtgctgggga
    14521 agggctcgaa ggagcgagat gtgcccatct ccgaatacgc ggccgacgcc ctgcgcgact
    14581 atctggaggc aggtcggccc cggatggccg ccgagggatc gcggcggctc ttcttcaatc
    14641 ggcgcgggaa acccttcagt gagcgcgata tccgttcgat ggttcagcgg tatggcgcta
    14701 ccctgctgcc cgggaggcgt gtcaccccac acacgctccg acactcgttc gccacgcatc
    14761 tgctggaagg aggggccgac atcagagccg tgcaggaact gctcgggcac gcgagcgtcg
    14821 cgacgacgca gcgatacacg cacgtctcgc gtcgccggct cttcgatgcc tacgagcgcg
    14881 cgcacccgag ggcctgagat ggcgacggcg aagaaggtgg ccacgaagcc ggcggcccgc
    14941 gcccggaaga cccctgccaa gaacgcgcgg gccaagacgg tcacgatccc cgctgtcgtc
    15001 gacgacgagt tggcgatccg ttgggacgtc ttcaagcgta cggccgacgg tgacgcaaga
    15061 gagaagctga tcctgcacta cgcgccgctc gtgaagtacg tagcgagccg cgtcgccagc
    15121 gggctgccct cgagcgtcga tcaacacgat ctcgtgagct acgggatgtt cgggctgatc
    15181 gacgccctcg agaagttcga gcccggacgg gggaacaagt tcgagacgta cgcgatccca
    15241 cggatcaagg gcgcgatcat cgacgagctc cgcgcgatgg actgggtccc ccggtccgtc
    15301 cgtttcaagg ccagggagct ggagaaggcg caggcggatc tcgaggcgat gttcaagcgg
    15361 cagccgaccg aagaggagct ggccgaccgg ctcggcatga atcgtcgcga gctccatgag
    15421 atggtgtcgc agatctcctt cgtctctgtg ctcgctctgg acgaggtcgt gaacgcgggc
    15481 agcgaccgcg gagagtcggt cgcgctgatc gacaccctcg ccgacaaggg gatcgatccg
    15541 acgtggggcg tggagagcca ggagacccgc gggatgctgg ccgctgcgat caactccctc
    15601 tccgagcgcg agaagatcgt cgtcacgctc tactacttcg agggtctgac gctcgccgag
    15661 atcggggaga tcctgggcgt gaccgagtcg cgtgtgtgcc agatccacac gaaagccgtc
    15721 ggcgggctcc gcggccaact ctccgagttc gactgacccc ggtccgtgtg acgcgcttca
    15781 cggcgcgcgg cgagcggacc tcctagcgtc ctgcgggtga ggaagctcgt cctgtggtgc
    15841 gcggtgtgga gtgcggtctt cgtgtccgcc gtgcctgcgc tggcgcgcgc ctcaggggat
    15901 tggacctggc cggtcgtcgg tcccgtgacc cgcgggttcg atccgcccga ttctccgttc
    15961 ggatcgggtc atcgcgggat cgacatcgcc gcgccggtcg gcacacccgt ccgtgcagca
    16021 accacgggca cggtcacgtt cgccgggccg gtgggcgggc gcctgttcgt caccgtggac
    16081 cacggcgccg gactcgagtc gacctactcg ttcctggact cgatcgcggt ccggagaggc
    16141 gacgtcgtgt cgccggggca ggcgatcgcc cactcgggga gggggcatgc gggcgacatc
    16201 gttcccggcc tgcacttcgg cgtgaagctc gccgacacct acgtcgaccc gctcgagtac
    16261 ctcggtccgg tcgaggtctg gcgcttcatc cgtctcgcgc cgctggagtc gtgagacgcc
    16321 cgctcgcagg cgaccggcta ggatgacgcg ccttgcgcgc cgcccaccgt tcccgtgtgc
    16381 cccgacgttc gacgaccctg gccgggtgca ccctcgccgt ggtgctcggc atgccgctcg
    16441 tggcgctcgc agagtggccc cagttccaag gcggcccgtc ccacgacggt ctgtccgacg
    16501 gtccctccgc cccgcttgag gtggcctggc gccacggcga catcgcgatc gacgatgcgg
    16561 acgtgacggg agggctctcg tcgccggtcg tcgcggacaa cggcaccatc gtggtcgtcg
    16621 ggccgcacga ggttctcggc ttcgacgacg agaacggctc ggaggtcttc acggcgccgc
    16681 gggatctcgg accgtccgtc acgccggcga tcggatcggg ccccgacggc caggtcgtgg
    16741 tgttcgcgga ggggttcggc gaccggggcc cggccaccac cgcatctccg accgcgtcct
    16801 cgacggcatc gccctcggcc tctccgggag aggagggtga caccggctcc cacctcgtcg
    16861 cgatcgatct ccggtcggga gagacggtat ggccttcgtc cgttcccctc gaggacgtcg
    16921 tccgcacccc ggtcacggcc gacgagaccg ccgtctatgt cggtgacttc ggcggtcacg
    16981 tcaccgccgt cgagctctcg tcgggggagc tccgatggtc gacggacatc ggcagctcga
    17041 tcgccggagc ggtgaccgtg gacggggatc gagcgctcgt cgccacggtc ggcgagcaac
    17101 agacgccggg ggcggtggtg gcgctcgacc gttcgacggg cgaggagctc tggcggaccg
    17161 gcgaggacgc ggtccagggg aacctcgtct cgggacctgt ggtcgcggac gggatgatcc
    17221 tcgtgctcga gcctggctcc gtcatcgcgc tcgatgcggc ggagggtcgg ttcctgtggc
    17281 ggtccgagat cgtcaacccc ctcacgacgc ccttctcccc ccaggggatc tccacgccgg
    17341 cgccggtctc ggccgacggc acggtctacg ccgtggacgt gaccgggcgc gcgtacgggc
    17401 tggacgccga gacggggacc gtgctctggg accaggcgct gaacgatccg tccccgctaa
    17461 cgccgcccgt gctcaccgtc gcgcacctct tggtgccgac gaactccggt tcgctctacg
    17521 cgatcgatcg cgagacgggg catctggtgt tcaagatcga cgccggagag gcattcctcc
    17581 gggggctcgc cgacgccggg gacgtcctcg tcgcggtcac cggcgtcgag gacgccgaga
    17641 tcgtggcgtt cggtgaagac ccggatggcg cactgacgga cgagccgtcc ccgaccacgt
    17701 tcgatctggg cagtctcctg gccgggttcg cgctgggcgg ccttccggtc tcgatcgtcg
    17761 cgctcgcgct cgcatcgccg ttgcagcggc gcctcggtcc cacaccgaga ccgggctcgg
    17821 atctcgatcc ggaggagggg gcgggatgag ctcggactcc cgtggcgccc ggggacggtc
    17881 ggctcctgcc cggcggggcg gcctgttgtc gcgtatgtcc gtcccgccca tggactcgat
    17941 gccgccgatc cgcacggcgt tcgcccgcgg cctggcggca acctggtcca gtccgctcgt
    18001 cgtcggggcg acggtcgcgt ggctcttgat cgagtggctc gtcgtcgtcg cgctcggcta
    18061 cccggggccg ttcgcgctct tggctcacat cgttgcgccc gcgcccctca gcaccaccac
    18121 ggacctctcg gtctcgatcg ggatcctcgg cgtcggcaag gggctcccgc tggtcctgat
    18181 ccctgctctt gtgcacgcgc tgttgcatgc gatcgttgtg gggctcgccg tggaggcggt
    18241 cgagaccggt cgtgccagcc ggtggggggc gatccgtggg ttgcgagctc tccccgttgc
    18301 gttcgcgatc cacgccttcg gtgtcgccgt gttgttcacg tcgcagatcg tggcggggtt
    18361 cggaggcggg ggcctctcgt tcatcctgca gctctcgatc ctggtcatcg cggtctgggt
    18421 gttcgcgttc gcaccagtga tcgcggtcac ggagggacgc cggttcctcg actgcctcgg
    18481 tcgcgccctc cgagccgctc gcctgccggg atcggggaac ctgaccttcg ccgtgctcta
    18541 cgtcgtcccg tggttcgcga tctccgtggc gacgatcgtc gggtccgtcc caggatcggg
    18601 actcggcgtg aaccccgcgt acacggcctg ggtcttcgtg ctcgcggtga gcctcctcca
    18661 caccgcgatc atcggggcca tcgcgatccg atacctggcg atcgcggacg aggtaccgga
    18721 tgcccccgtc cgcgcggcgc cggaacgggg cgcgaggtcc cgcggggcgc gatcgggctc
    18781 gggccgccgg tagcacgcat cgccgctggt aaactgcgcg cgccggtgcc ggagggcgcc
    18841 gagcacgcac gtccgaggac cgtgacggcg gggtcccaag ccccaagaac cagcagaggc
    18901 ctgggccgcc cgcgggaaga tccggacgga ggaagaaacc aacagcagga gggaaacgtg
    18961 cctgtcgtca ccaggcggga gctcctcgag gccggcgttc acttcggcca ccagaccaga
    19021 cggtggaacc cgaagatgca gcgctacctg ttcggggagc gatccggcat ctacatcatc
    19081 gatctcgaga agtccctcgc cggcatcgag gagacgttcg aattcgttcg cgatctcggc
    19141 cgccgtcgcg gcacggtcct gttcatcggg accaagaagc aggcccagga ggtcgtcgag
    19201 gagcatgcgg gccgcgtcgg catgccgttc gtgaacaacc gatggctcgg cggcatgctc
    19261 acgaacttca gcaccatctc caagcgattg gtccggctcc gggagctgcg ggagatggag
    19321 tcctccgacg cgatggacaa cctccccaag aaagaggtca tccgcctcag gcgcgagcgc
    19381 gagaagctcg agcgcaacct cgggggcatc caggcgctcg agcggctccc cgacgccgtc
    19441 ttcgtggtcg acacgaagaa agagcagatc gcggtgaccg aggcgcgcaa actcgagatc
    19501 cccgtgatcg cgatcgtgga tacgaactgc gatccggacg aggtggacta cgtgatcccg
    19561 ggcaacgacg acgcgatccg cgccgtgagc ctcgtcgctc gggtggtcgc ggacgcgctc
    19621 gccgaaggct acgggatggc gaaggacgag gtggtcgaga agtccaccgc acgacgcccc
    19681 acggcggcac cgcgccggtc cacgaccgcc gccccggctc ccgcggacga ggacgtgccg
    19741 tccgccgagg acgccgccgc gatcgcggcc agcacggtct tcgagcccga tctcccgacc
    19801 ccggagaccg ccgaggagga gccggaggtg gtcgccgaac ccgcgatcga tgaaccggcg
    19861 tctgccgctc catcgagggg cgaggcatga tcgccatctc cgccgaccag gtgaagatgc
    19921 tccgcgagcg gaccggcgcg gggatgatgg actgcaagcg cgcgctccag gagaccgacg
    19981 gcgacctcga gagggcgatc gagctgctgc gcgagcgggg actggcgtcg gtcgccaagc
    20041 gagccgaccg tacggccgat cagggtgtgg tcgacagcta catccatttc aacaacacgg
    20101 ttggcgtgct ggtcgaggtc aacagcgaaa cggacttcgt ggcgaaaacg gacgagttcc
    20161 ggcagctggt gaaggacatc gcgctccata tcgcgagccc ggccgctccc cgctggctca
    20221 cccgcgagga cgtccccgcg gacgtcatcc gagcggagga gcacatcgcc ctggcgcagg
    20281 ccaaggaggc cgggaagccc gagaacgtgc tcgacaggat cgtcgagggc aagctcaacg
    20341 ccttctacca aggctccgtg ctcctggagc agccgtacgt gaaggacgac gcgaagacga
    20401 tccagcagtt gctcgatgag gtcgccgcca aggtcggcga gaaggttgcc gtccgtcgat
    20461 tcgtccgata caagcttggt gaatccaccg aggcctgatg gccctcgacg ccgacgttgc
    20521 cgggacgatc acgaggggag cgcacgcgaa cgtgacggag aggagtcccg gggggacggg
    20581 ataccaccgc gtcctcctca agctctccgg cgaggcgttc gcacccaccg acggcatggg
    20641 gatcagcacg gaggcgaccg cgctgatcgg ccggcagctc gccgaggtcg tcgacctggg
    20701 cgcacaggtc gcggtcgtgg tcggtggcgg caacatctgg cggggtcggc aggcccccgc
    20761 gatcgagcgg agccgggccg actacatggg gatgctcgcg acggtgatga actcgttggc
    20821 gctgcaggac tcgctcgaga agctgcacgt gaccacacgg gtgcagaccg cgatccagat
    20881 gacgcagctc gccgagccgt tcatccctct tcgggcgcga cgtcacctcg agaaggggcg
    20941 cgtggtgatc ttcgctgccg gtctcggcgt cccgtacttc tccaccgaca cctgcgcggc
    21001 acagcgagcg ctcgagatcg gtgcgaacgc gctcctgaag ggcacgaagg tggacggcgt
    21061 gtatacggcc gaccccaagc tcgtcccggg ggcctcgcgc ttggcggagg tcggctacgt
    21121 cgacatcctc cgcgagggct accaggtgat ggacgccacg gcgatctcgc tctgcatgga
    21181 gaacgacctg ccgatcatcg tcttcgacct ctgggaagag gggaacatcc gccgcgtggt
    21241 gatgggtgag accgtgggtt cgctggtcca tggagggacg agatgacgat cgattcgtcc
    21301 ctcaaggagg cggaccacaa gatggagcag gcgctcgccc atctccggga ggatctctcc
    21361 tctgtccgaa ccggccgggc ctcgggatcc gtgctcagcc ggatcaccgt ggagtactac
    21421 ggaacgcccg ttcccctgaa tcagctcgcc tccgtgaacg tgcccgagcc ccgcctgctc
    21481 gtcgtccagc cgttcgacaa gaactcgatc gcacagatcg agaaggccat ccagcagagc
    21541 gacctcggga tcacgccgtc gaacgacggg caggtgatcc ggctggcgtt ccctccgctc
    21601 acggaggagc gccggaagga cctcgtgaaa caggtccacc atcgagcaga ggagggtcgc
    21661 gtcgcggtcc gcaacgtccg ccggcacgcg aaggatgata tggagaagct tgagcgggag
    21721 cattccatct cggaagatga gctctcgagg ggagagaagg agctccagaa gctgacggac
    21781 cgatacgtgg cgtcgatcga cgaggtgcag cgccacaagg aatctgagct catggaggtc
    21841 tagtgcccga cgacgagcgc gagagcgacg acctgttcga agatctcgac aagttcttcg
    21901 ccccgatcaa ggacgtcgat tgggacgagc gggagggggc tccggcaacg acggcctcac
    21961 cgggcccgtc ggtatccgag gaagagcacg tcacggtgcg gagcgagccc gagcaggtgg
    22021 tctcggtgcc ggcagcggac gatgagacgc ccgcgacggt cttcgatgat gacgatgatg
    22081 atgacgacga cggggactgg tacgacaccg cgtcgctcga acccgtgggg caggccctgg
    22141 gaggttccgt cggcaacgac gacgacgagg acgagatgcc gcccggtggc cccgcggtga
    22201 tgcaggatgc agaccaggac gagacggatc tgttcgcacc gcccgccgcc gccgatgacg
    22261 actggcccgc cgacgaggtc gaggaccagc aggcggcgcg tccctccgac gcggagctcg
    22321 aggaagcagc ggcgcacttc gcggagtcgc tcggcgatga gggcaccgca gccgcacccc
    22381 gcgtcgtcca gatcgacgag gagaacgcga ccgatctcat ggcggagctc ggggccgagc
    22441 cctatccgcc ggatgagcag gaggacgtct tcgccgcgat ggccgaggag gagccgtccc
    22501 gaacgatcgt ggtcggcgga gaaggtctcg gcgggccgag ctggcaagag ccggccgcgg
    22561 tcgaggtcgg ggccgacctc gaccggcgcg ctgccgagcg ggacatgccg gcagcgttcc
    22621 tcacggggat cgtcctggca ggtgtcgcgg tggtcgcgct cctgatcggc gaaggcgtat
    22681 tcgcgatcgt cgcggccgtc gtggcgctcg ttgcgcaggg tgagttgtac ggggtgatgg
    22741 tgaagcacca ccggcaaccg gccaccgccg tgggtttggt gagcggtgcg ctcgtgctcg
    22801 cgggcgcgta ctaccacggt gaggcggggc tgacctcgat ggtgtccctg ggcctgctgg
    22861 ccaccttcct ctggtacctc acggttcctg caccgcaccg gaaggacctg gtcggcaaca
    22921 tcggtctcac gttgctcggt ctggtgtgga tccccgccct cgccggttcg ttgctgatca
    22981 tcctcaccac gttcggcgcc gaggtcgtgg tcgcggtgat cgcactcacg ttcgtcttcg
    23041 acaccgctgc cttcttgatc ggctcggtct ggggcggatc cttcgtccaa cggccgctcg
    23101 cgcctgcgac gagcccgaag aagtcgatcg aagggctcgt cggtgcgtcg ctgatcaccg
    23161 tgatcgtctc gatggcgttc atcacctcgt tcgtcgacgt ggcccccgag atcttctccg
    23221 gagcgcgcgt gaatgcggcg ttgctgggca tcgtcgtctc gctggcggcg acgttcgggg
    23281 acctcgcgga gtcactgatc aaacgcgacg tgggtgtgaa ggacatgggg agcgttctcc
    23341 ccggacacgg gggcgtcctc gaccggatcg attcgctcct cttcgtggcg ccggccgcct
    23401 tgctcctgct gcgggtcatc gccttctagc gacccgttca ggatcgtggc gttctggtcc
    23461 ggttgttcct tgttcccttg cgcgcggggg tagtctgttc ccatgcggag cgtcaccatc
    23521 ctcggatcga cgggctcggt cgggacccaa gcgctggatg tggtccgtcg caatcccgag
    23581 cggttcaagg tcatcggctt gtccgcagcg gggacgaacc aagagctcct cgccggccag
    23641 atccgcgagt tcctccctcc ggtcgtcggg atcgccgatg agcaggcggc ggaggaggtc
    23701 cgcacgcacc tcggctcgat ccggggtgtg gacttcatcg tcgggcccga cgcggccgaa
    23761 cgcctggcat cggaggccga agcggacatc gtgctgaacg cgctcgtggg ctcgtcgggg
    23821 ctgggtccca cgctcgccgc gctccagagc gggaagaccc tggccctcgc caacaaggaa
    23881 tcgctcgtcg tcggtggtga gctggtcatg gacctgatca agggtgagcc ggagcggctc
    23941 ctgccggtcg actccgagca cgcggcgctc gcgatggcgc ttcgcggtga gcgccgagaa
    24001 gacctcaagc gtgtcgtgct cacgggctcg ggcggcccct tccgcgggtg gaccgccacc
    24061 gagctggcca aggcctcgat gaaggaagcc ctgcagcacc ccgtctggtc gatggggccc
    24121 aagatcacga tcgactccgc cacgctcatg aacaagggtc tcgaggtgat cgaggcgcat
    24181 tacctcttcg gcctcgagta ttcgcagatc cacgtgctca tccatccgga gggcctggtc
    24241 catgcgatcg ctgagttccg cgacggctcg atgcgagccg agatggcgac ggcggacatg
    24301 cggctgccga tccagctcgc cctggcgtgg cccgagcggc tgccgacggg cgtcgcgtcc
    24361 ctccccctca cggagtcgcc gttcacgttc gagcccgtcg atcacgaggc cttcccggcc
    24421 gtggacctcg cctatcgggt cggcgggctg ggactcacgt tccccgctgt gatgaacgcc
    24481 gcgaacgagg tcgccgtgat ggcgttcctc gaggggaaga tcccactgcc gcggatcgtc
    24541 gagatcgtcc agacgatcgt cgacgagcat gaaccaccct cggtcgtctc ggtcgtgcac
    24601 atcgagcgcg cggacacctg ggcccgccgc cgcacggtgg agatcatcga ggctcggtag
    24661 gtgggcggcc cgctcgcgat cgtcgggttc ctcgcactga tcttgctcgt cgtgatcccg
    24721 atccacgagc tcggccacct cttggtcgcg agacggttcg ggttcaagat ccaggagtac
    24781 ttcgtcggct tcgggccgaa gctctggtcg atgaggcgtg gcgagacgga gtacggcatc
    24841 aaggcgatac cggccggcgg ctacgtgaag atcgccggga tgaatccgta cgagaccgtt
    24901 ccggcccaag atgtcgcgcg cgcgtacggg tcgaagccga tctggcagcg cgcactggtt
    24961 atcctggccg gtccgttgtc gcacgcgctc gtcggcgcgc tcctgtactt caccctgttc
    25021 gtcacgtacg gggatccgaa caccgagctc gtggtggtgg gcgaggtcgc cccgacgctc
    25081 gacgggagct cctcgccagc gctcgaagcg gggctccagg tcggcgatgt cgtgacccgt
    25141 gtcggggacc tggagggtcc gacgcccgat tccctcggcc gctacgtcac ccggttcgcg
    25201 gaggaccgtc ccggcgagcc gctcacgtac gtgatcgaac gcgacggccg tcacctcacc
    25261 ctcgagatcg tcccgcgtct cgtcacggag gacgagatcg cgaggggacg gatcgggttc
    25321 acgctcggcg ccgaggacct gtcgatcccc gggtcggtgg tggagtcggg ggagtgggtc
    25381 ggcagagccg cgtggaacag cgtgacgacc atcccgaagg tcttcacggt gggcgtcggt
    25441 cgcacgttct cggtgttgtt caccgacgag ccgcgacgcg acgacgacgt gacgagcctg
    25501 gtgggcgtca gccggcagat cggcggtgcc ggcgagcgtg gggattgggg attgttcctg
    25561 aacttcgtcg cgtacgtcac gatcttcatc ggcgtcgtga acctgatccc gctcccgccg
    25621 ctggacggcg gtcacctcct gctcctggcg tgggagaagg tgacgggccg gcaggccgac
    25681 taccgcaagc tcgtccccgt ctcggcggcg gtcatcgtct tcctctcggt cttcgcgatc
    25741 gcgacggtct tcctcgacat cacggagccg ctcccgctca tctagggtcg cgtcgctcac
    25801 tccttgagga ccgaggggcc gggtcgccct caccacgatt tctccatccc gcgaggcgct
    25861 gtcgacgggg tggggtggat acgatggcgt cgtggccgct cagaccgtgc tcgtcgtcga
    25921 ggacgaggac gcgatcgccg aagccgttcg ggcccgtctc cggagcgagg gatatcacgt
    25981 cgtcgtcgcc tccgacggtc ccgaggccct tcgtcgacac gccgagaccc gtcccgacct
    26041 cgtggtcctc gacctgatgc ttccggggat ggatggtctc gaggtatgca cgcggatcca
    26101 gcggaacggc tggaccccgg tgttgatgct caccgcgaag accgaggagg cggacaaggt
    26161 ggccgggttc gcggtcggcg ccgacgacta cctgaccaag ccgttcagcc tccgagagct
    26221 gtccgtccgt gttcaggcga tcctccgccg gcgggagcgg atcgagacct cgtcgaacgg
    26281 agagccgatc gagcacgagg gactgatgat cgacgccaag cggcggcggg tggaagtgga
    26341 cggctcggag atcccgctca cgccgttgga gttcgagatc ctgctcacgc tcgcgcggga
    26401 acccggcgtc gtcctgtccc gcgatcagct catggaccgg atctggggct accgggacca
    26461 cgccggcggg cgggtggtgg actcgcacgt ggcgcgcctc cgacgcaaga tcggggagga
    26521 cggcaacgat ccgcggttca tccgaaccgt ccacggcgtc gggtacgcgt tcaaggagtg
    26581 acgatggcca cgcgcccgtt ggatcggctc a
//
LOCUS       DASUYD010000112         4453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_278926, whole genome shotgun sequence.
ACCESSION   DASUYD010000112 DASUYD010000000
VERSION     DASUYD010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278926"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VF195_06130"
     CDS             <1..375
                     /locus_tag="VF195_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEX6580429.1"
                     /translation="RFPTIQGPPREDICYATQNRQDAIKEVARRSDVVLVIGSDNSSN
                     SNRLAEVARDLGTPAYLVDDESEVDPAWLDGADVIGLTSGASAPERLVDRLLAWLAAQ
                     GATEVEELRLREEHLRFSDVRV"
     gene            complement(510..1700)
                     /locus_tag="VF195_06135"
     CDS             complement(510..1700)
                     /locus_tag="VF195_06135"
                     /inference="COORDINATES: protein motif:HMM:NF014010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HEX6580430.1"
                     /translation="MDEQPKSVKDLLVEAKDASELMVDLAYAAVFFNDEKLAHEVERL
                     EERMNGHLRRLREVAMLAARSPEDAEGMAGVLWIADAVEKIGDAASDIARVVEARLGM
                     PEALRRDLRHADEMTARVKVREGAQVIGRALRELSLPTETGMWVMAIRSGIEWQFDPG
                     PDDVLSEGDVLLVRGPEEGVTLLRELAGAPPRPEPPDDDAPPLSELDRAVDILIELKD
                     QSEAAVGLAYSSILFNNRALAAEVGALEARSDALHDELESWVLRAAPEARNPDELRGL
                     LRLGSASESMCDAARDLTWYVEHGEPLHPVIQLALEETEETSAETVVEAGTPADGKSL
                     KDLRLETETGMFVLAVQRGARWIYRPRGPFVLHAGDRLIAVGPEEGAEELDALCRVAA
                     PTAS"
     gene            complement(1693..2931)
                     /locus_tag="VF195_06140"
     CDS             complement(1693..2931)
                     /locus_tag="VF195_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6580431.1"
                     /translation="MPDVGLARRARSLWAYWRAERRTRRQGLIALVLSTLASFVAGLT
                     LGAITGTLEEFPGLFILIPASVGMRGVISGTTGARLGTSIAAGVFEVSWRSGVLKQNA
                     FLGLVLTLSSSLYVAALARLSAVAFGEPSISLLDLVTISVVGGTIGSALVLVATIGLA
                     VVSFRRGYDLDAVGTPVITAVGDMVTLPALFLATFLVRSGGPSDVIAIFCLAGAAVGI
                     VAGVRAEPTIRRTVIEMTGVLLIVPILDVLAGTLLESQSARFFLYPGLLILVPPLMSQ
                     AGALGGILCSRLSSKLRLGVITPRGRPEPPAVVDLSLVVTFGIAIFTFIGVLGFALAD
                     LVGKATPGAATTVWGTVLAGLMLLPIILGLGYYLAVGTTRFGLDPDNHTVPLTTGVMD
                     LAGVVAFVVSMSLLGVAFHG"
     gene            complement(2924..4156)
                     /locus_tag="VF195_06145"
     CDS             complement(2924..4156)
                     /locus_tag="VF195_06145"
                     /inference="COORDINATES: protein motif:HMM:NF013895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6580432.1"
                     /translation="MAFPRRVVDMRASLRAEQRTLRQGLVALVLSTAAGFVAGLTLAH
                     FTDRLELLPGLIVLIPAAVGMRGTIFGAIGARLGTASAAGTFRPTLARDGVLFRNVDV
                     AILTTLSSSLWLAVLAKITTVVLGQPSISIWSFVTISVVGGVLGSALILFVTVGLSVL
                     SFRHGWDLDAVSTPMVTALGDMATLPTLFLASVLIEVPAVNAIVSGLCLVAGLAGAVQ
                     AYRSKVPGVRRIAVEMNAIILLTPLLDVLAGGLLQGHEAQLIAVPTVLLIVPPFVSQA
                     GALGGIFSSRIASKLQLGVIRPRGWPAPAVWLDASLVVAFGLVVFTLIGVVALALAVL
                     TQLAHPGVGELIGGTLLAGGLTLPLMLVVSYEVAVWTTRFGLDPDNAGVPIITSVMDL
                     AGIASLLFAMSVFGVTRA"
BASE COUNT          788 a         1531 c         1535 g          599 t
ORIGIN      
        1 cggttcccca cgatccaggg gccgccccga gaggacatct gctacgcgac ccagaaccgc
       61 caggatgcga tcaaggaggt ggcgcgccgc tccgacgtcg tgctcgtcat cggttcggac
      121 aactcgtcga actccaaccg cttggccgag gtcgcgcgcg atctcgggac gcccgcgtac
      181 ctcgtcgacg acgagtccga ggtcgatccg gcctggctcg acggcgccga cgtgatcggg
      241 ctcacgagcg gagcgtcggc tcccgaacgc ctggtggatc gtctcctcgc gtggctcgcc
      301 gcccagggcg cgaccgaggt cgaggagctc cggctgcggg aggagcacct tcggttctcc
      361 gacgtccgcg tctgacgccg ccgcagtgcg cggcgcggtc tcatcctcga ccttccctgg
      421 agtgaacccg cagctagacg atcgcgccca cgccggaagg ctaggtccgc gaacgcgggg
      481 aggcaaggac aaccgagtgg caggggagcc tacgaggccg tgggtgcggc cacccgacag
      541 agcgcgtcga gttcctccgc gccctcctct ggtccgaccg cgatgagccg atctccggcg
      601 tggagcacaa acggcccgcg gggccggtag atccacctcg ccccccgttg caccgccagg
      661 acgaacatgc ccgtctcggt ctccaaccga aggtccttca ggctcttgcc gtcggccggg
      721 gtccccgcct ccacaacggt ttcggccgag gtctcctcgg tctcctccag cgcgagctgg
      781 atgaccgggt gcagcggttc accgtgctcg acgtaccacg tcaggtctcg ggcggcgtcg
      841 cacatcgact cgctggcgct gccgagtcgg agcaaccccc tcagctcgtc ggggttgcgt
      901 gcctccggcg ccgcccggag cacccaggac tcgagctcgt cgtgcagggc gtcgctccga
      961 gcctcgagcg ctccgacctc ggcggccaac gctcggttgt tgaacaggat cgaggagtac
     1021 gcaaggccca cggccgcctc ggactggtcc ttcagctcga tgaggatgtc gacggccctg
     1081 tccagttcgg agagcggcgg agcgtcgtcg tcgggtggtt cggggcgtgg cggggcgccg
     1141 gccagctcgc gaagcaacgt cacaccttcc tccgggccac gcacgagcaa gacgtcacct
     1201 tcggacagca cgtcgtcggg tccggggtcg aactgccact cgatcccgct ccgtatcgcc
     1261 atcacccaca tgcccgtttc cgttgggagc gagagctcgc gcagtgcccg cccgatcacc
     1321 tgggctccct cccgcacctt cacgcgggcg gtcatctcgt cggcgtggcg gaggtcgcgt
     1381 cggagggcct cgggcatccc gagacgcgcc tcgacgacgc gggcgatgtc cgaagcagcg
     1441 tcgccgatct tctcgaccgc gtccgcgatc cacaggacgc cggccatccc ctcggcgtct
     1501 tcggggctgc gtgcggcgag catcgcgacc tcacgcagcc gcctgaggtg gccgttcatc
     1561 cgctcttcga ggcgctcgac ctcgtgggcg agcttctcgt cgttgaagaa caccgccgcg
     1621 tacgcgaggt cgaccatgag ctcggaggcg tccttggcct cgacgagcag gtccttcacg
     1681 ctcttcggtt gctcatccat ggaaggcgac tcccaatagt gacatagaga ccacgaaggc
     1741 gacgacgccc gcgaggtcca tcacgccggt cgtgagcggg acggtgtggt tgtcgggatc
     1801 caggccgaac ctggtcgtcc cgacggcgag gtagtagccc agtccgagga tgatcggcaa
     1861 gagcatgaga ccggcgagca ccgtgcccca caccgtcgtg gctgcgcccg gcgtcgcctt
     1921 gcccacgagg tccgccagcg cgaacccgag caccccgatg aaggtgaaga tcgcgatccc
     1981 gaacgtgacc acgagcgaca ggtcgacgac ggcgggtggc tcggggcgtc cgcggggagt
     2041 gatgacgccc aaccggagct tggaggacag gcgcgagcac aggatccccc cgagcgctcc
     2101 cgcttgcgac atcaaagggg gtaccaggat caacagtccc gggtagagga agaaccgcgc
     2161 cgactgcgac tcgaggagcg tgccggccag cacgtcgagg atgggcacga tgagcagcac
     2221 gcccgtcatc tcgatcaccg ttcgccggat cgtgggttcg gcccggacgc cggccacgat
     2281 gcccacagca gctcccgcga ggcagaagat ggcgatgacg tcgctcgggc cgccggatcg
     2341 gaccaggaac gtcgcgagga agagcgccgg gagggtcacc atgtctccca cggcggtgat
     2401 cacgggcgtc ccgaccgcat ccaggtcgta gccgcgacgg aacgagacga ccgcgagccc
     2461 gatcgtcgcc acgagcacga gtgctgaacc gatcgttccc ccgaccaccg agatcgtcac
     2521 gaggtccagg agcgagatcg agggctcgcc gaaggcgacc gctgaaaggc gcgcgagggc
     2581 tgctacgtac agggatgagc tgagcgtcag gaccaacccc aggaaggcgt tctgtttgag
     2641 cacgccgctc ctccacgaga cctcgaagac gcccgccgcg atgctcgtgc cgaggcgcgc
     2701 tccggtcgtt cccgagatga cgccgcgcat gccgaccgag gcggggatca agatgaacag
     2761 gccggggaac tcctcgaggg ttcccgtgat cgcaccgagc gtgagtccgg cgacgaagga
     2821 cgcgagggtc gacaggacga gtgcgatgag gccctgccgt cgcgttcggc gctcggcgcg
     2881 ccagtacgcc cacagactcc gggcgcgccg ggccagcccg acatcaggca cgcgtgaccc
     2941 cgaacacgct catcgcgaag aggagcgatg cgatgcccgc gagatccatc acgctcgtga
     3001 tgatcggcac gcccgcattg tcggggtcga gcccgaagcg ggtggtccag acggccacct
     3061 cgtagctgac gacaagcatt agcggaagcg tcagcccgcc ggccagcagc gtgccgccga
     3121 tcagctcacc cacgcccgga tgagcaagct gtgtaaggac ggcgagcgcg agtgcgacaa
     3181 cgccgatcag cgtaaacacg acgaggccga aggccacgac gagcgaggcg tcgagccaga
     3241 ccgcaggggc cggccaccca cgtgggcgga tgactccgag ttggagcttg gaggcgatcc
     3301 tcgacgagaa gatgccgccg agcgctcccg cttgcgagac gaagggcggg acgatcagca
     3361 ggaccgtcgg aaccgcgatc agctgtgcct cgtggccctg gagcagcccg cccgccagca
     3421 cgtcgaggag cggcgtgagg aggatgatcg cgttcatctc caccgcgatg cgccgaacac
     3481 ccgggacctt cgatcgatac gcctgaacgg cccccgcgag gccggccacg aggcagagac
     3541 ccgagacgat ggcgttcacg gcgggcacct cgatcagcac cgacgcgagg aacagcgtcg
     3601 gcagcgtcgc catgtcgccg agagcggtga ccatcggcgt cgagaccgcg tcgaggtccc
     3661 acccgtggcg gaacgagagg accgagagcc cgaccgtcac gaacaggatc agggccgaac
     3721 ccagaacgcc tcccacgacc gagatcgtga cgaagctcca gatcgagatg gacggctggc
     3781 cgaggacgac ggtcgtgatc ttcgccagga ccgccaacca gagcgacgat gacaacgtgg
     3841 tgaggatcgc aacgtcgacg ttgcggaaca gcacaccgtc acgagccaag gtcggccgga
     3901 acgtccccgc agccgacgct gtgcccagac gcgctccgat ggccccgaag atcgtcccgc
     3961 gcatgcccac ggcggccggg atcaagacga tgaggcctgg aaggagctcg agacggtcgg
     4021 tgaagtgggc gagcgtcagg ccggcgacga agccggcggc cgtgctgagg acgagcgcga
     4081 ccagaccctg gcgcagggtc cgctgttccg cgcgcaggga ggcacgcata tcgacgactc
     4141 gacgcgggaa cgccataacg gttcgctcat cgtgtccttc ttctcgatgc cggctcgtgc
     4201 tgggtcaacg gatccccggg ctccgggagc ccgaggcagt atgcgcccgg cgtccttgcg
     4261 ggacaacccg ccgcgttgcc tcggagaggc tcagtccacc cagtcgcggg taaggaagag
     4321 ccagaaggcg aggccggcgt acgggccgta gcgacggaac cggcgttcga tccccgcgtc
     4381 cgtgacgctc gggcggccaa ccttcttcgc gtacgtcggc cgggtccacg aatcgaggat
     4441 cagccggtgg tac
//
LOCUS       DASUYD010000113        17957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1822282, whole genome shotgun sequence.
ACCESSION   DASUYD010000113 DASUYD010000000
VERSION     DASUYD010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1822282"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="VF195_06150"
     CDS             <1..457
                     /locus_tag="VF195_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6580433.1"
                     /translation="SLWTRGPEAEVLPTLEELGIGFVPFSPLGRGFLTGKMDVHMTFD
                     SSDFRAALPRFTPHAREANQALVDLLRTIGDRKGATPAQIALAWLLAQRPWIVPIPGT
                     TKLERLQENLGAAEVGLRSEDLVEIDRAASEIRVEGARYPEHLEQMTGR"
     gene            complement(357..1994)
                     /locus_tag="VF195_06155"
     CDS             complement(357..1994)
                     /locus_tag="VF195_06155"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580434.1"
                     /translation="MIPETRYAKTSDGIHIAYQVLGDGPIDLVYVGPWVTHIEYRWEL
                     PQYASYLRRIASFSRLIMFDKRGFGMSDPVPADQLPNLETRMDDLRAVVDEVGSDRAV
                     IYGASESGMLDLLFAATYPERTLALVIHGSYPSVRWEPEAPWGWREDEFAERLAAIER
                     SWGTEEFFIRWAFPELAQDPEILRWFTTYTRRGASPGAVIAAERMELDTDVRDILSAI
                     HVPTLILHRVEDDPEANRYMAEHIAGAEYVTLPGNEHIPYLGDQDSALKEIERFVKGI
                     QAEQASLDRVLATVLFTDVVGSTEKAAELGDSAWRDMLEQHHATVRRLLAHYRGTEVD
                     TAGDGFFATFDGPARAIRCAQAILEAVARLGLEVRAGLHTGECETIAGKVGGIAVNIG
                     ARVGSIARPSEVLVSRTVKDLVAGSGLMFEDRGMHELRGVPDECTSMRSSEGETEYIR
                     PIRGVTLRAATPDHLDPPIVLHVDDTRSTPSGVFRLPGWTASSSSRRTQLPRRALSSR
                     AARRLSDRSSAPGVRGIERPPPGSPRPRGRFRRDPRT"
     gene            2333..3115
                     /locus_tag="VF195_06160"
     CDS             2333..3115
                     /locus_tag="VF195_06160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6580435.1"
                     /translation="MSDGHVRVNRDSWDQDAPNWVERGRDAWALEEPVWGRGNLESEV
                     HLLPDVSGLDAIELGCGTAYISAWLMRRGARVIGLDNSSRQLATARTFQQEFDLHFPL
                     VHADAERVPFADESFDFAISQYGAAIWCDPYRWIPEASRILRPGGRLVFETGSPLVML
                     CFPTDDDEAPADSQLHRDYFGMHRFEWHDQKGVVDAIEFRLGHSDMIRLLRSCGFEIE
                     DLLELQPPPDASLEMDPYIPLEWARRWPSAEIWKVRRVRQQP"
     gene            complement(3503..3925)
                     /locus_tag="VF195_06165"
     CDS             complement(3503..3925)
                     /locus_tag="VF195_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580436.1"
                     /translation="MATSTAVKPYVVQDSVVAPQAETRSGWVTFAGVVLLVGAVGNLF
                     WGLAALDSKSYLDETGLLYSTLETWGWVAIGWSALLLIGGIMLLARARYGPAVGIVLA
                     AVSCVFWLFALPVMPFYAMTVMFVDLLVIYGLVSGSTE"
     gene            4088..4663
                     /locus_tag="VF195_06170"
     CDS             4088..4663
                     /locus_tag="VF195_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580437.1"
                     /translation="MMDRKGRGAEGGTGVVLIVAEPPCPPSLYCGDRAMKETVSFESL
                     PRMVATSDLVIEGTVRIVEPGRIVGAGDASIQFAQVTVSVDRVLFGRWDAVNVVVEEY
                     GLERGHPSRTGDHGIYFLHQKADAPMFQRLVNSQGRFLDDGKGGLVAPNDEADWVKAI
                     ESEPLSQLQSDVRAAARAVAEGRVEPAKPII"
     gene            4881..6665
                     /locus_tag="VF195_06175"
     CDS             4881..6665
                     /locus_tag="VF195_06175"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEX6580438.1"
                     /translation="MESSSETKHQLDKEPTRPDDLFTTETTGLSDAARPELIRLRDGD
                     RFDLRIEPVRKRLGDSELRMLGYNGSIPGPTLHVDQGSEITVQVTNDGDVEATVHWHG
                     LRLENRYDGVPHETQSPIPIGGTFTYKVQFPDAGFYWYHPHIREDFGLEMGLYGTIIV
                     EPSDPEYWPAVDRQLSITLDDLLVEDGRIAPFHRAGPNYTAMGRFGNVMLINGDTELS
                     GEATVGEVVRLYLVNTANTRIFNFSLPGARMKLVGGDSGRYERETFIDEVMLAPSERA
                     VLDVRFDNPGKVRLEHRTPDHVYDLGAFAVVGDASAVTAESFEALRVDPQLTAEHRSM
                     AADIERPPDKVLAFVASMPLLYGDDVAPATSYACPMHPEVTSSEPGTCPKCGMQLLPV
                     RPDESTPASYVCPMHTEVTSPEPGTCPECGMKLVPADTLPASAPKPHEEAVHHEQDHE
                     QSDGLEWEDLMPEINRASEPSNMRWMLIDRETGAENGAITWAFTVGDRVKIRLVNEME
                     SDHPMHHPFHVHGAGRFLVLSRDGEPEPNLVWKDTVLVPAGRTVDILLDVSNPGLWMA
                     HCHIAEHAQSGMMFSFDVSRRDPSEVAT"
     gene            6662..7762
                     /locus_tag="VF195_06180"
     CDS             6662..7762
                     /locus_tag="VF195_06180"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6580439.1"
                     /translation="MTSGNGAPSFDVVVIGGSQAGLATGYHLAQRGLSFVILDAGPEV
                     GHAWRSRWDSLKLFTPAQYSGLPGMAFPSPRDTYPSKDDVASYLQTYASAFDLPIKLN
                     AKVTSLTKRDGGYLVTIADGGFTASNVVVATGPFQIPFVPPAADELDETVTQIHSADY
                     RNPAQLPDGHVLVVGGGNSGFQIAEELAATRKVDLAVGKRVPSLPQRLLGKDLFWWLS
                     GVGFMKVSTDSRLGRRLSERDVLIGSSARRLRRSGVTMRARLTSAVGRRALFEDGSDQ
                     DVDAVVWATGYRPDFSWIDVPDVKGASDRIVHRRGVTDAPGLFFVGLTWQHTRGSALI
                     GFVNDDAAFIAERIDQHLEDRTGRVGMDSERT"
     gene            7774..7923
                     /locus_tag="VF195_06185"
     CDS             7774..7923
                     /locus_tag="VF195_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011222411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6580440.1"
                     /translation="MAMVTDPVCGMNIDSEEAAGTIEYEGTTYYFCSQTCHDAFEADP
                     TSYVT"
     gene            7973..8743
                     /locus_tag="VF195_06190"
     CDS             7973..8743
                     /locus_tag="VF195_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580441.1"
                     /translation="MTGDRLTEDEVAERVGATPGRIRLLAEMGILEPEDGTYPRRDVL
                     RARFVLGLEAIGVDAAAVADALASGDLTLGYVEFGGRRPPRSDRTFAELSDEIEIPYP
                     TLERMYVALGLPRPTPEEYVREEDLRLIRFIPVLFGAGVSEGEVLRLARVWGDNARRI
                     AQFQSHYLHHSIEEPFHRRGLRDNEAFEAALREVGVRAGRSGEDLLGWLFGVIRRCSR
                     PGTSSIMSRPRSKTRGCTGGNLGRSKLACSRTFPATPR"
     gene            8710..8961
                     /locus_tag="VF195_06195"
     CDS             8710..8961
                     /locus_tag="VF195_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580442.1"
                     /translation="MFADLSGYTTLTEESGDEMAALVSLKLAELVNEIASRHRGAVVK
                     MLGDGVHFHFKDPSDAVMASLEIVDRASIPRACRQRTSA"
     gene            8973..9188
                     /locus_tag="VF195_06200"
     CDS             8973..9188
                     /locus_tag="VF195_06200"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580443.1"
                     /translation="MIYDEGDYFGRTVNIAARIASQAGPDQVFVGEDVLRHITTSGFR
                     LTEVGAFELKGIAKPLVLYEAIHDGDA"
     gene            complement(9303..9854)
                     /locus_tag="VF195_06205"
     CDS             complement(9303..9854)
                     /locus_tag="VF195_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6580444.1"
                     /translation="MLEMVMWKAIATLVIGLALVLVAIGVAFFIGMRTGSPLVRNVVR
                     RFNRAFGNPHQMKTAGTPGAYASFIRHVGRKTGRSYETPMVPFATEDGFVIALPYGSG
                     TDWVRNVMASGSATIVHEGNTYRVNQPELVPTAMAAPYMPAKEQRELRRFAIDQCLRV
                     RRAVPGRGVRTQETRAVTASPTA"
     gene            10492..10779
                     /locus_tag="VF195_06210"
     CDS             10492..10779
                     /locus_tag="VF195_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580445.1"
                     /translation="MTRQIMYVNPPIGIWIPYVSALNRSTAATICAFSALSKGCEAPD
                     VAKIDEAHPPGVGAITDKLRLRENAYTANAPWRRPRLRRPSHEMKCQQGCE"
     gene            complement(10812..11474)
                     /locus_tag="VF195_06215"
     CDS             complement(10812..11474)
                     /locus_tag="VF195_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580446.1"
                     /translation="MLEIVVSIAIVVAIFGWGIPKFADYGAVRDALSTLTPIEFVTLF
                     AAMVFNLITYWWANQAALPGLGIGKAAVLTPDHDVGREHDPLGWRDRGWSDVLDPGLV
                     GVPRNEHRPVRGCHWPWNIFTKLGLPMLALVLLALDGHLTPAYIAGAVVGAAVIAAAV
                     LLLALVFKSERLAIRIGEGLGRAMSTVRRLLRHPSVTGWGQGAAKFRRDTIAWRSTGG
                     SG"
     gene            complement(11594..13219)
                     /locus_tag="VF195_06220"
     CDS             complement(11594..13219)
                     /locus_tag="VF195_06220"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580447.1"
                     /translation="MRTLGRLCLATVVMLAIGVGSAAASNDQVTAEIRKGTLIVEGTD
                     VGEEIVLRLRAGDATTLEVVTPDGILAFARDSFTRIAVEADDGDDLLRIDQTNGAFTD
                     TEATTLSGGHDDDDLLGGAGVETFLGGPGDDEIDGNQGTDEAFLGSGDDSFTWDPGDG
                     SDKVEGQPGDDTLIFNGAGVSESFDASANGKRLRFFRDLGNIVMDVDGTERIDLRALG
                     GTDITVMNDLRRTDVEAFDIDLSPTIGGITGDGQADTVALFGSDSPGRRDDVQINAFS
                     DAVRVTGLSADVSVVHPEAASDLLSVNTLGGNDEVAGSLGLAARLKLAVDGGAGNDTI
                     LGGDGADLLLGGEGDDDIDGNAGADVAHLDAGDDSFTWDPGDGSDLVEGEDGTDTMIF
                     NGSAGNEAFELLANGERLTLTRNLGNIVMDVNDVEQVDLEALGGTDLTTVNDLSGTDV
                     RDINVDLEGVLGGGAADGAADTVTVNATDQVDVIDVASVDGETHITGLTATVRITHAD
                     PLLDKLIVNTLGGVDQVNVGAGVAALIGATVNQ"
     gene            complement(13428..16319)
                     /locus_tag="VF195_06225"
     CDS             complement(13428..16319)
                     /locus_tag="VF195_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580448.1"
                     /translation="MSGSAHPTGSALLERDGELARLAAALDRARDGGGSFVVVEGPAG
                     MGKTAVLTGSRAIADARGMRVLRGRGAELEREFAFGVVRQLFEPVLASASVVEREDLL
                     KGAAGFAARALGLPGAPSGTLEESASRADWSFAVLHGLYWLCANLASGHPLYIVVDDA
                     QLADAPSLRYLAFLLPRLEELPVALAIATRTHETAAGWWLLETITGDASAEVVRLLPL
                     SRAGVRDFVEEALDAPPEPEFVDACLRATRGTPFMLHELAGALRDEGVKPTAELATHV
                     DRIGAHTIGRSLSLRLSRLSEPAVRLARSVAILERSDLDQAARLAEISRDDAIEAADV
                     LAVAGILDPGRPLRFVHPMVRAGIYEGLSTGTRARSHRSAARLLGDVSGENERVAEHL
                     LASEPAADAWSVDRLVEAARAAARTGAPETASVYLRRALAEPPKPNERPGILLELGMA
                     EASAGLADWRRHLEAAVETAADDGARVDAAMVLGLALNRAQDGAAAIEVLDRTASSLD
                     RADQERRLLLEAAAAGVEIANAVAAPGVVGRRRAVRDRANADPSAPPELLAVAALIAV
                     LTNEPARVGAELAHRALTAERDRLATGSRRPWFSYATWFAQATGSLLWAERYDELRPL
                     LDESIAHAPATGDSGRLAIGLALRGWLALRLGDLAAAEVDALTALAASELRAPALYRV
                     LNGGVLVNALLEQGELDAAQAALAPFDGEIESDYLAAAVIRFGRGRLRIAQGRIDEGL
                     ADLLGVGALTTRALVTCPSYLPWRSEAALAHLLVGNHDEAKVLADQELELARAFAAPR
                     ALGVALRAAALVAGGQAGEALLRESVAMLEPAGARVDLAWSLTELGALIRRANRRSEA
                     RELLRQALDTAHRLGARPLARRAETELRATGARPRRIVLTGLESLTASERRVAELAAQ
                     NLTNREIAQGLFVTSRTIEGHLTSVFRKLHLASRDELPAALAGDAPAVA"
     gene            16483..16623
                     /locus_tag="VF195_06230"
     CDS             16483..16623
                     /locus_tag="VF195_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580449.1"
                     /translation="MAEKRTTAYGRSGKKWLAIYAIVGAVAFLIVYLVFFSEGGGTGG
                     LY"
     gene            16940..17317
                     /locus_tag="VF195_06235"
     CDS             16940..17317
                     /locus_tag="VF195_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6580450.1"
                     /translation="MTERELARAAAHPLTIIRHAQEVTGNVSLTCRYFGITRQAYHIW
                     LRRYEEQGLEGFRDRSRRPHVSPNATRAEVVGKIVYLRQSYHFGPHKIAMYLKRYHDR
                     GQFRGRPRLPITRRTNYTGGCPG"
     gene            17750..>17957
                     /locus_tag="VF195_06240"
     CDS             17750..>17957
                     /locus_tag="VF195_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6580451.1"
                     /translation="MPTCPNCGEDNPERARFCMGCTAPLVAVASRGKRRTVTIIFSDL
                     VGSTALGERLDAEALREVLDRYFEE"
BASE COUNT         3021 a         5932 c         6013 g         2991 t
ORIGIN      
        1 ctcgctgtgg accagaggac ccgaggccga ggtgttgccg acgctcgagg agctcgggat
       61 cggtttcgtc cccttcagcc cgctcggtag ggggttcctc acggggaaga tggacgtgca
      121 catgacgttc gacagcagcg acttccgggc cgcgctccca cgcttcactc cgcatgcgcg
      181 ggaggcgaac caggcactgg tcgacctgct ccgcacgatc ggagaccgga agggtgcgac
      241 gcccgcacag attgcgctcg cctggttgct cgcccagcgg ccgtggatcg tcccgatccc
      301 ggggacgacg aagctggagc gtctgcagga gaacctcgga gcggctgagg tcggactcag
      361 gtccgaggat ctcgtcgaaa tcgaccgcgc ggcctcggag atccgggtgg agggcgctcg
      421 ataccccgaa cacctggagc agatgaccgg tcgctgaggc gtcgggcggc gcgcgagctg
      481 agggcccggc ggggcagctg cgtcctcctc gaggatgacg acgctgtcca acctggtagc
      541 cgaaagaccc ctgaaggagt ggaccgtgtg tcatccacgt ggagcacaat cggcggatcg
      601 agatggtcgg gggtcgccgc ccgtaatgtg acacctctga tgggacggat gtactcggtt
      661 tcaccctcgg acgaccgcat agaggtgcat tcgtccggga caccccggag ctcgtgcata
      721 ccccgatcct cgaacatgag cccggacccc gcgacgaggt ccttcacagt ccttgacacg
      781 aggacctccg aggggcgagc tatcgatccg acccgcgcgc cgatgttcac ggcgatgccc
      841 ccgaccttcc ccgcgatcgt ctcgcactcg ccggtatgaa gcccggcccg aacttcgagg
      901 ccgaggcgcg caacggcttc caggatcgcc tgcgcacagc ggatcgcacg agccggacca
      961 tcgaacgtgg cgaagaaccc atcgcctgcc gtatccacct ccgtcccccg gtagtgcgcc
     1021 aacaagcgtc gaacggtcgc gtgatgttgc tcgagcatgt cgcgccaggc gctgtctccc
     1081 agctcggctg ccttctcggt ggaaccgacg acgtcggtga aaagcacggt ggcgagtacc
     1141 ctgtctaggg acgcttgctc agcttgaatc cctttcacga agcgctcgat ctccttcagg
     1201 gcgctgtcct ggtctcccag gtaagggatg tgctcattac cgggcaaggt gacgtactcg
     1261 gcgcccgcga tgtgctcggc catgtaccgg ttcgcctccg gatcgtcctc gaccctgtga
     1321 aggatgagag tcggaacgtg gatcgccgac aggatgtcgc gcacatcggt gtccaactcc
     1381 atgcgctcgg ccgcgatcac cgcgcccggg ctcgcgccgc gacgtgtata agtggtgaac
     1441 caccgcagga tctcggggtc ctgggccaac tctgggaatg cccaccggat gaagaactcc
     1501 tcggttcccc aggaccgctc gatagccgca agccgctcgg cgaactcgtc ctcgcgccat
     1561 ccccacggcg cctcgggctc ccacctgacc gacgggtacg agccgtggat cacaagtgcc
     1621 aacgtccgct ccggatatgt ggccgcgaag agtagatcca gcatcccgga ctcggaagcg
     1681 ccgtagatca cggcgcggtc cgagccgact tcatccacga cggcgcggag atcgtccatt
     1741 cgagtctcca ggttggggag ctggtcggcg ggtaccgggt cagacatccc gaagccgcgc
     1801 ttgtcgaaca tgatcagcct cgagaacgac gcaattcggc gcaggtagga cgcgtactgg
     1861 gggagctccc atcggtactc gatatgcgtt acccacggcc cgacgtagac gaggtcaatg
     1921 ggtccgtcgc ccagcacttg gtaggcgatg tggatgccgt ctgaggtctt cgcgtagcga
     1981 gtctcaggga tcacgcggat agtttctggc gaggggcttc gtggcgcaat acggacctgc
     2041 cagcggttat cccgcgacga tcaggccttg ggcacgcgcg cgctttccct cacggcgtag
     2101 gagtagggaa catttgtccg cggattgcga cgggtgggta gcgcaggtat atgaagacag
     2161 tcgctgaacg cgacgctccc agccattgat ccgccccgga cggaagccca tgcgcccgcg
     2221 tgaggccttg gccattcgct aggtctacga cagggggcta tgtcggcgga tcgagatgat
     2281 gagggccggc atgcattcaa gaggaagagg tttcctccgg tatggtgcgc gcatgtccga
     2341 tggccacgtt cgcgtgaacc gggactcgtg ggaccaggat gccccgaact gggtcgaacg
     2401 aggacgagac gcgtgggcat tggaggaacc ggtctggggc agagggaatc tcgagtccga
     2461 ggtccatctg ttgccagatg tatccggact ggatgcgatc gagctcgggt gcggtaccgc
     2521 ctacatctcg gcgtggctga tgcgccgagg cgcacgcgtg atcggtctcg acaactcgtc
     2581 gagacagctc gcgacggccc ggacgttcca gcaggagttc gatctgcact ttccgctcgt
     2641 gcacgcagac gccgagcgtg tgccgttcgc tgatgagagc ttcgacttcg cgatctccca
     2701 gtacggagcc gcgatctggt gcgacccgta tcggtggatt cccgaggcct cccggatctt
     2761 gcgtcccggt ggacgcctcg tgttcgagac gggctctcca ctcgtgatgc tctgcttccc
     2821 gacggacgac gacgaagcgc ccgccgactc gcagctccat cgcgactact tcgggatgca
     2881 tcgcttcgag tggcacgatc agaagggcgt cgtcgacgcc atcgagtttc gcctcgggca
     2941 cagcgacatg atccgactgc ttcgttcgtg cgggttcgag atcgaggatc tgcttgagct
     3001 ccaaccgccg ccagacgcct ctctcgagat ggatccgtat atcccgctcg agtgggcgcg
     3061 acggtggccc agcgcagaga tctggaaggt acggagggtg cggcagcaac cctgagtcac
     3121 cctgggcggt ctgcgcacaa ggaacgtcgt cgggggaacg cgaggaccgc cgacgacgtg
     3181 gatcgtgtgt cggtgcagcg tctgcgtcac ggtctctgcg gcccgatcct cgcccaccgc
     3241 cgctcaagcg ttagcggacg gtgcggcccc ccacgggatc ctgtccggtc tggtgcccga
     3301 cgtcgcgaga tacgtcccgg ggcgaccagt ttcgcggtcg tcggcatcaa cgcggccaca
     3361 ttgctgacgt cggtgctcgg cgcgtttgcc gtcgtgtggt cgccgcggga ggcgtgctga
     3421 tacggcgccg ggacatcact gagacgattc gtagacccgg cggccctcac cgtgagggcc
     3481 gccgggtcag gcggattgcc gtctactcgg tcgatcctga cacgaggccg tagatcacca
     3541 agaggtcgac gaacatcacg gtcatcgcgt agaacggcat cacgggcagc gcgaacagcc
     3601 agaagacgca gctgacggcc gcgagcacga tcccgaccgc cggcccgtac cgggcccgcg
     3661 cgagcagcat gatgccaccg atcagtagca gcgcgctcca gccgatcgcg acccaacccc
     3721 acgtctccag ggtgctgtac agcagcccgg tctcgtcgag gtagctcttg ctgtcgagcg
     3781 cggcgagccc ccagaacagg ttccctacgg cgccgaccag caacacgacc cccgcgaacg
     3841 tgacccaccc tgaccgtgtc tcggcctggg gcgccacgac gctgtcctgc acgacgtacg
     3901 gcttcacagc ggtggacgtg gccatctctt ctccttcccg actccgagca accaccggcc
     3961 catgcctgca gggccggctc aaccgtaggc gcgcgcgcga gctgcctcca gggccgaagg
     4021 gaccccatcg ggcgggcctc ccggcgcccc gcgagcgagt gcaaagcaga tcgcgacggt
     4081 cctccacgtg atggatagga aggggcgggg ggccgaaggt ggtacggggg tcgtcttgat
     4141 tgtcgccgag cccccgtgtc ctccgtccct ttactgtggt gaccgcgcca tgaaggaaac
     4201 ggtcagcttc gagagcctcc ctcggatggt cgcgacatcc gacctggtga tcgagggcac
     4261 cgttcggatc gtcgaaccgg gccggatcgt gggtgcaggt gatgcttcga tccagttcgc
     4321 tcaggtgacc gtgtctgttg atcgcgttct cttcggaagg tgggacgccg tcaacgtcgt
     4381 agtcgaggag tatgggctgg aacgcggtca cccttcacgc acgggtgacc acggcatcta
     4441 cttccttcac cagaaggcgg acgccccgat gttccagcgg ctcgtgaaca gccaggggag
     4501 attcctcgat gacggaaagg gcgggctcgt tgccccgaac gatgaagccg attgggtaaa
     4561 ggctatcgag agcgagcctc tctcccagct acagagtgac gtccgggctg cggccagggc
     4621 cgtggcagag ggaagggtcg agcccgctaa gccgatcatc tagccgcctc cagaagtcgt
     4681 gttccggcaa gcccttgctt cgtagctcgg acccgacccc caggttgtgg gtttgtcgtc
     4741 aagacgtagt ctgtggagtg acgccaaaca accgggttcg cgccgccact gacgtcaatc
     4801 tcgggctcgt acccgaaacc acatttctcg gcccgggcca acgagatcga gagagttcgg
     4861 aacgaagcat gggggctgcc atggagagct cgtcggaaac caagcatcag ctcgataagg
     4921 agccaacgag acccgacgac ctgttcacga ctgagacgac tggactctca gacgcggcgc
     4981 ggccggagct catccgtctc cgcgatggtg accgattcga cctgcgcatc gagccggtgc
     5041 gcaagcgctt gggcgactcc gagctccgga tgctcggata caacggctcg atccccggcc
     5101 ccaccctgca cgtcgatcag ggatcggaga tcaccgtgca ggtgaccaac gacggtgacg
     5161 tcgaggcgac ggtccactgg cacggcctac ggctggagaa ccgctacgac ggcgtcccgc
     5221 acgagaccca gtcaccgatc ccgattggag ggacgttcac gtacaaggtg cagttccccg
     5281 atgccgggtt ctactggtat cacccgcaca tccgcgagga cttcggcctg gagatgggcc
     5341 tgtacgggac gatcatcgtc gagccctccg acccggagta ctggccagcc gtcgatcggc
     5401 agctcagcat cacgctggac gatctgctcg tcgaggatgg ccggattgcg cccttccacc
     5461 gtgcgggccc caactacacc gcgatgggac ggttcgggaa cgtgatgctg atcaatggcg
     5521 atacggagct ctcgggtgag gcgacggtgg gagaggtcgt acgcctctac ctcgtgaaca
     5581 cggcgaatac gcggatcttc aacttctcgc tgcccggtgc tcggatgaag ctggtcggtg
     5641 gtgacagcgg tcgctacgaa cgcgagacgt tcatcgacga agtgatgctc gctccctcgg
     5701 agcgggcggt cctggacgtg cggtttgaca accccggaaa ggtgcggctc gagcatcgca
     5761 caccggatca cgtctacgat ctcggtgcgt tcgcggtcgt cggggatgcg agcgccgtca
     5821 ccgcggagtc gttcgaggcc ctacgcgtcg atccccaact cacggctgag catcgatcga
     5881 tggctgccga catcgagcgg cccccggaca aggtgctcgc gttcgtggcc tcgatgccgc
     5941 tcctctacgg ggatgacgtt gcgcctgcga cctcgtacgc ctgtcccatg cacccggaag
     6001 tcacgtcctc ggagccggga acctgcccga aatgcgggat gcagctcctc cccgtccgtc
     6061 ctgacgagtc tactcctgcc tcgtacgtct gtcccatgca tacggaggtc acgtccccgg
     6121 agccgggaac gtgccccgag tgcgggatga agctggtgcc ggcagacacc ctgccggcgt
     6181 ccgccccgaa gccgcatgaa gaggccgttc accacgagca ggaccacgaa cagagcgacg
     6241 gactcgaatg ggaagacctg atgccggaga tcaaccgtgc ctccgagccc agcaacatgc
     6301 gatggatgct catcgaccgg gagaccgggg cggagaacgg ggccatcacc tgggcgttca
     6361 ctgtaggcga ccgggtgaag atccggctcg tgaacgagat ggagtccgat cacccgatgc
     6421 accacccgtt ccacgttcac ggcgcgggtc gcttcctggt cctgtcgcgc gacggtgagc
     6481 cggagccgaa cctggtctgg aaggacaccg tgctcgtccc ggcgggaagg accgtggaca
     6541 tcctgctcga cgtgtcgaat ccgggcctgt ggatggccca ctgccacatc gccgagcatg
     6601 cccagagcgg gatgatgttc agcttcgacg tgtctcgccg tgatccttct gaggtcgcga
     6661 catgacctcc gggaacggag cgccttcgtt cgacgtcgtc gtcatcggcg gcagccaggc
     6721 aggcctcgcc accggctatc acctcgctca gcgtggcctg agtttcgtga tcctggatgc
     6781 cggcccggag gtcggccacg cgtggcggtc ccgctgggac tcgctcaagc tcttcacacc
     6841 ggcgcagtac agcggccttc cggggatggc cttcccttcc ccgcgagaca cctacccgtc
     6901 gaaggacgac gtcgcctcat atctccagac atacgcatcg gcgttcgacc tcccgatcaa
     6961 gctcaacgcc aaggtcacat ctctcacgaa gcgtgatggc ggatacctcg tgacgatcgc
     7021 ggacggggga ttcacggcga gcaacgtcgt cgtggcgacg ggtcccttcc agattccgtt
     7081 cgtgccccct gcggccgatg aactggacga gacggtcacg cagatccaca gcgcggacta
     7141 tcgaaacccg gctcaactcc ccgacggtca cgtgctcgtg gtgggcggag gcaactctgg
     7201 cttccagatc gccgaggaac tcgccgcgac gaggaaggta gatctcgcgg tgggcaagcg
     7261 agtcccttcg ctcccgcagc gcttgctcgg caaggacctt ttctggtggt tgtccggggt
     7321 cggattcatg aaggtcagca ctgattcgcg gctcggtcgg aggttgtccg aacgagacgt
     7381 gctcatcgga tccagcgcga ggaggctccg gcgctccggc gtgacgatga gagcgcggtt
     7441 gacgagcgct gtggggcggc gcgccttgtt cgaagacggg agcgatcagg atgttgacgc
     7501 cgtcgtctgg gcgacggggt atcgacccga cttctcctgg atcgatgtcc ccgatgtcaa
     7561 gggagcgagc gaccggatcg tccatcgacg cggggtgacc gacgctcccg ggttgttctt
     7621 cgtcggactc acctggcagc acacgcgggg gtcggcgctc atcgggttcg tgaacgacga
     7681 tgctgcattc atcgcggagc gtatcgatca gcacctcgag gaccgtaccg gacgtgttgg
     7741 gatggattcg gagcgcacgt agaggagaac cgtatggcga tggtgacgga tcccgtatgc
     7801 gggatgaaca tcgacagtga agaggctgcc gggacgatcg agtacgaagg aacgacgtac
     7861 tacttctgct cgcagacttg ccacgacgcc ttcgaggcgg atccgacctc gtacgtgacg
     7921 tgagaccaag gatctcctgc cggctggtcg atcctgacgt tcgggctcga cggtgaccgg
     7981 tgatcggctc accgaggatg aggtcgcgga gcgtgtgggg gcgactcccg gccgtatccg
     8041 actgctcgcc gagatgggca tcctcgagcc cgaggacggg acctatccgc gacgggacgt
     8101 gctgcgagcc cgtttcgtcc tcggtctcga ggcaatcggc gtcgatgcgg ctgccgtcgc
     8161 ggatgcgctc gcgtcggggg atctgaccct cgggtatgtc gagttcgggg ggcggcgtcc
     8221 acctcgttcg gaccgcacct tcgccgagct cagcgacgag atcgagatcc cctacccgac
     8281 ccttgaacgc atgtacgtcg cattagggct accgcgcccg acacccgaag agtacgtgcg
     8341 cgaggaggac ctgcgtctca tcaggttcat ccccgtgctc ttcggggcgg gtgtgagcga
     8401 aggcgaagtc ctgcggttgg cgcgcgtgtg gggcgacaac gctcgccgga tcgcgcagtt
     8461 ccagagccac tacctccacc actcgatcga ggagccgttc caccgtcgcg gcctccgcga
     8521 caacgaggcc ttcgaggcgg cgctcaggga ggtcggcgtc cgggccggtc gatctgggga
     8581 ggacctcctt ggctggctct tcggcgtcat tcggaggtgt tcgagaccgg gcacctcttc
     8641 gatcatgtcg agaccgcgct cgaagacgcg ggggtgcacc ggagggaacc tcggcaggtc
     8701 gaagcttgcg tgttcgcgga cctttccggc tacaccacgc tgaccgagga gtccggggac
     8761 gagatggcgg cgctggtgtc cctcaagctc gccgagttgg tcaacgagat cgcttcccgg
     8821 caccgtggtg ccgtcgtgaa gatgctgggt gatggcgtgc acttccactt caaggacccg
     8881 agcgacgccg tgatggcctc actcgagatc gtggaccgcg cgtccatccc gagggcttgc
     8941 cgccagcgca cgtcggcgtg aatgcaggtc cgatgatcta cgacgagggc gactatttcg
     9001 gccggacggt caatatcgcg gctcgcatcg cgtcgcaagc cggaccggat caggtcttcg
     9061 tcggagagga cgttctacgt cacatcacga cgagcggatt ccgcctcaca gaggtcggag
     9121 ctttcgagct gaagggcatc gcgaagccac tcgtgctcta cgaggccatc catgatggcg
     9181 acgcctagct ttccccaggg caacgggccg tcgatacgag cggccatcac gtggggcatc
     9241 gtcgtaagcg tgctccaggc agcgtcgccc tcgggcttct ggtggctgga tccggctgcc
     9301 gtctatgcgg tcggactcgc cgtgaccgcc cgcgtctcct gtgtcctgac gcctcgccct
     9361 gggaccgcgc ggcgcacccg gaggcattgg tcgatggcga accggcgaag ttcccgttgc
     9421 tccttggccg gcatataggg cgcggccatg gctgtgggaa cgagttccgg ctgattgacc
     9481 cggtacgtgt tcccttcatg gacgatagtg gcggacccgc tcgccatgac gttcctgacc
     9541 caatcggtac ccgacccgta aggcagcgcg atcacgaacc catcttcggt cgcgaaggga
     9601 accatcggcg tttcgtagga tcggcccgtc ttccggccga cgtgtcggat gaaggaggca
     9661 tatgcgcccg gggtcccggc cgtcttcatc tggtgcgggt tgccaaaggc ccggttgaac
     9721 cggcggacca cattgcgcac gaggggcgat cccgtccgca tcccgatgaa gaacgcgacc
     9781 ccgatcgcca cgagcacgag cgccaggccg atgacgagcg ttgcgatcgc tttccacatg
     9841 accatctcca gcatcttcat cacctctctt cgagtttgag cggcgagccg cgctgctcac
     9901 agggtcgaag gcctagtaga gccagggacc tgccctggtg tccgggcgtc ccctcgaggc
     9961 cccgtcttgg ggctgttgtc ggcggatcga gttgtgagag gcctagatcg tccgcgacct
    10021 agctcgccac tcgttcgacg aagcgtgcgc attccgcccg aacgatcggg ccacggcaag
    10081 gagcccactt ccctcatgag tcgacaacga gcctgctagg tatccctgac cgaccagaag
    10141 gatcgccgac gccccggctt tccaccttcc ggccagacaa acaggtcact gcgcgagcgc
    10201 tcggctgaac cgcggaacag cgaaccgcgt gtcgtcgatc acgatgctgt aagcacgaag
    10261 atcgtgtgtc atctccacgg cgcggaatcg gtggatcgag agggtgggaa gcgcgggcac
    10321 gaagagagtc gctagggccg cttcgcggtt gcccgatgtg ttccccgacc gagggctatc
    10381 cgcccccggc gcaggcggcc acagcgacgg tgagacctcg cgtgtctagg tcgcgaagta
    10441 gctgacgcga tcgcctgcgc tgccgcgtcg gccggccgac gccagtccga cttgacccgc
    10501 cagatcatgt acgtgaaccc gccgatcggg atctggatcc cgtacgtgag cgccctgaac
    10561 aggagcacgg ctgcgacgat ctgcgccttc tcggcgctgt cgaagggctg tgaggccccc
    10621 gatgtagcca agatcgatga ggcccaccca cccggtgtag gggcgatcac cgacaaactg
    10681 cgactgcgag agaacgcgta cacggcgaac gctccgtggc ggagacctcg gcttcggaga
    10741 ccctcccatg agatgaagtg ccagcagggg tgcgaataac aacgcgacct ggctcagaac
    10801 ggtcgtccac gtcagccgga tccacctgtg ctccgccagg cgatcgtgtc gcgccggaac
    10861 tttgcggcgc cctgtcccca gccggtcacc gatggatgcc gcagcagccg tctgaccgtc
    10921 gacatcgctc gaccgagacc ctcgccgatc cggatcgcga gccgttcgct cttgaacacc
    10981 aacgcgagga ggaggaccgc cgcggcgatg accgcggccc cgacgaccgc cccggcgatg
    11041 taggccggcg tgaggtggcc atcgagtgcg agcaggacga gggcgagcat cggaagcccg
    11101 agcttggtga agatgttcca cggccagtga cacccacgta cagggcgatg ctcgttccgg
    11161 ggaaccccca cgagtccagg atcgagtacg tcagaccaac cgcgatcgcg ccacccaagg
    11221 ggatcgtgtt cgcgaccgac gtcgtggtct ggcgtcagga ccgcggcctt gccgatccca
    11281 agtcccggga gcgcagcctg gttggcccac cagtaggtga tgaggttgaa caccatcgcg
    11341 gcgaagagcg tcacgaactc gatcggcgtg agggtggata gcgcgtcccg tacggccccg
    11401 tagtcggcga acttggggat cccccacccg aagatcgcca cgacgatcgc gatggagacc
    11461 acgatttcga gcaccgtctt ccagcgcggc cgcgtccctt gcggcgagga catcaccgcc
    11521 aggttggcac gcgagatggc agaagtcggg gcccccagcg gaggccccga cttgcaccct
    11581 ggaaggccgc gctctactgg ttgaccgtcg ctccgatcag tgcggccaca cccgcaccaa
    11641 cgttcacctg atcgaccccc ccgagcgtgt tcacgatcaa cttgtcgagg agtggatcgg
    11701 cgtgtgtgat gcggacggtg gccgtgagtc ccgtgatgtg ggtctcgccg tcgaccgagg
    11761 ccacgtcgat gacgtcgacc tggtccgtgg cgttcacggt caccgtgtcg gctgcgccat
    11821 cggccgcgcc acctccgagc acgccctcga gatcgacgtt gatgtcccgc acgtcggtcc
    11881 ccgagaggtc gttgacggtc gtcaggtcgg taccgccgag cgcctccaga tcgacctgct
    11941 cgacgtcgtt gacgtccatc acgatgttcc ccaggttgcg cgtcaacgtc agccgctcac
    12001 cgttcgcgag gagctcgaag gcctcgttcc cggccgagcc attgaagatc atcgtgtcgg
    12061 tgccgtcctc gccctcgacg agatcgctcc cgtcgccggg atcccaggtg aaactgtcgt
    12121 cgccggcgtc cagatgcgcg acgtcggcgc ccgcgttgcc gtcgatgtcg tcatcgcctt
    12181 ctccgccgag caggaggtcg gctccgtcgc ctccgaggat cgtgtcgttt ccggcgcccc
    12241 cgtcgacggc gagcttgagt cgcgcggcga ggccgaggct gccggcgact tcgtcgttac
    12301 cgccgagcgt gttcacgctc agcagatcgc tggcggcctc ggggtggacg acgctcacgt
    12361 cggccgacag gccggtcacc cggaccgcgt cggagaaggc attgatctgg acgtcgtcac
    12421 ggcgtccggg cgagtcgctg ccaaagagcg ctaccgtgtc cgcctgaccg tcgccggtga
    12481 tccccccgat ggtgggggag aggtcgatgt cgaacgcctc gacgtccgtc ctcctcaggt
    12541 cgttcatgac ggtgatgtcc gtacccccga gggctcggag atcgatgcgc tcggtaccgt
    12601 cgacgtccat gacgatgttg ccgaggtcac ggaagaagcg aagccgcttc ccgttggcgg
    12661 aagcgtcgaa gctctcgctc acgcctgcac cgttaaagat cagcgtgtcg tctcccggct
    12721 gtccttcgac cttgtcgctg ccatcgcccg gatcccacgt gaagctgtcg tcaccggagc
    12781 ccaggaaggc ttcgtcggtg ccctggttcc cgtcgatctc gtcgtcgccg ggcccgccca
    12841 ggaacgtctc cacccccgcg ccgccgagga ggtcgtcgtc gtcgtgcccg ccgctgaggg
    12901 tggtcgcctc ggtgtcagtg aaggccccgt tcgtctggtc gatgcggaga aggtcgtccc
    12961 catcgtcggc ctcgaccgcg atccgggtga acgagtctcg ggcgaaggcg aggatgccgt
    13021 ccggcgtgac gacctcgagg gtcgtcgcat cccccgcgcg caggcggaga acgatctcct
    13081 ctcccacatc cgtgccttcg acgatcaggg tccccttcct gatctccgcc gtcacctggt
    13141 cgttggacgc cgctgccgac cccacgccga tcgcgagcat cacgacggtc gcgaggcaca
    13201 gacgcccgag tgtgcgcatg tgctgcatca tttgcctccc tccgatccgg gccgcggctg
    13261 tcgcggccgg tctcgcttcg ttgcgcatcc acgagatcgc ggatgcgggc cgctctcggc
    13321 cctggcgtgc ttcatggctc gagctccttt cctgtcatgg gaacggcatc gtgaagcccg
    13381 gggcagaccc ttggcaccgg ggccaccccc tggtctttgg agccggacta cgcgacggcc
    13441 ggggcgtcgc cggccagggc ggcggggagc tcgtctcgcg acgcgaggtg gagcttgcgg
    13501 aagacgctgg tgaggtgacc ctcgatcgtc cgggatgtga cgaagagtcc ttgggcgatc
    13561 tcgcggttcg tcaggttctg tgcggccagc tcggcaacac gccgctcgct cgcggtgagc
    13621 gactcgagtc ccgtcaggac gatgcgccga ggccgtgccc cggtcgcgcg cagctcggtc
    13681 tcagctcgtc gggcgagggg ccgagcgccc agccgatgcg cggtgtcgag cgcctggcgc
    13741 agcagctcgc gcgcctcgga tcggcggttc gcgcgccgga tcagagcccc gagctcggtg
    13801 agcgaccacg cgagatcgac ccttgcgccg gcgggctcga gcatggccac ggactcccgc
    13861 agcagtgcct cgcctgcctg cccgccggcc acgagcgccg cggcgcgcag cgcgacgccc
    13921 agagcgcgcg gtgctgcgaa cgcccgcgcc agctcgagct cctgatcggc gagcaccttc
    13981 gcttcgtcat ggttcccgac caggagatgg gcgagcgcag cctcagatcg ccagggcagg
    14041 tagctcgggc aggtgacgag cgcccgcgtc gtcagtgcgc cgacgcccag aaggtcggcg
    14101 aggccttcgt cgatccgacc ctgggcgatc cgaagacgtc cgcggccgaa ccggatcacg
    14161 gccgccgcca ggtagtcgct ctcgatctcg ccgtcgaacg gcgcgagcgc cgcctgcgcc
    14221 gcgtccagct cgccctgctc gagcagtgcg ttcacaagga cgccgccgtt gagtactcgg
    14281 tatagcgccg gagcccggag ctccgaggcg gcgagggcgg tgagcgcatc cacctcggct
    14341 gcggcgagat caccgaggcg gagggcgagc catccgcgga gggccagccc gatcgcaagc
    14401 ctgccgctgt cgccggtcgc cggcgcgtgg gcgatcgact catcgagcag aggccgcagc
    14461 tcgtcgtagc gctccgccca cagaagcgaa ccggtggcct gcgcgaacca ggttgcgtag
    14521 gagaaccaag gccgtcggct cccggttgcg agccggtcgc gttcggcggt cagcgcccgg
    14581 tgcgcgagct cggcgccgac cctggccggc tcgttcgtca ggaccgcgat caacgctgcc
    14641 accgccagga gctcgggagg tgcggacggg tcggcattcg cccggtcgcg cacggcccgc
    14701 cgccggccga cgacacccgg cgccgcgacg gcattcgcaa tctcgacccc ggccgcggcc
    14761 gcctcgagca gcaggcgacg ctcctgatcg gcacgatcga gcgatgacgc ggtgcgatcg
    14821 agcacttcga tggcagcggc cccgtcctgc gcacggttga gcgcgagtcc aaggaccatc
    14881 gctgcatcga ctcgggcccc gtcgtcagcg gcggtctcga cggcggcctc gaggtgacga
    14941 cgccagtcgg ccaggccggc gctggcctcc gccattccga gctcgagcag gatgcccggc
    15001 cgctcgttcg gcttgggagg ctcggcgagc gccctgcgga gatagacgga ggcggtttcg
    15061 ggagcgcctg tgcgtgcggc agcccgagcg gcctcgacca agcggtcgac gctccacgcg
    15121 tcggccgccg gctcactggc gagcaggtgc tcggcgactc gctcgttctc gcccgacacg
    15181 tcgccgagca gccgggctgc ggagcgatgg ctccgagcac gtgtccccgt cgaaagaccc
    15241 tcgtagatcc cggcgcgtac catcggatgg acgaacctca gcggacgacc cggatcgagg
    15301 atgccggcga cggccaggac gtctgcagcc tcgatcgcgt cgtcacggga gatctccgcc
    15361 agccttgcag cctggtcgag gtcgctccgc tcgaggatcg cgacggagcg tgcaaggcgc
    15421 accgcaggtt cggagagacg actcaggcgc agcgagagcg agcgaccgat cgtgtgggct
    15481 ccgatgcgat cgacgtgcgt ggccaactcg gccgtcggct tgacaccctc gtcgcgtagc
    15541 gctccggcga gttcgtgcaa catgaacggc gtgccgcgtg ttgctcgtag gcacgcgtcg
    15601 acgaactccg gttcgggagg ggcgtcgagc gcctcctcta cgaagtcacg gacgccggcg
    15661 cgtgacaacg gcaggagccg gacgacctcg gccgacgcgt cgccggtgat cgtttcgagc
    15721 agccaccaac cggcagcggt ctcgtgcgtt cgggtagcga tcgcgagcgc caccgggagc
    15781 tcctccagcc gggggagcag gaacgcgagg taacgcagcg atggcgcgtc ggccaattgg
    15841 gcatcgtcga ccacgatgta cagcggatgg cccgaagcca ggttcgcaca cagccagtac
    15901 aggccgtgaa gcaccgcgaa cgaccaatcg gccctcgagg cggactcttc gagcgttccg
    15961 ctcggtgcgc cgggcagacc gagggctcgc gctgcgaaac ccgccgcacc cttcagcaga
    16021 tcctcgcgct cgacgaccga cgcactcgcg agtaccggct cgaagagctg cctgacgacg
    16081 ccgaacgcga actcccgctc cagctccgca ccccgtcccc gcagcacgcg catgccacga
    16141 gcgtctgcga tcgctcgaga ccccgtgagt accgctgtct tgcccatccc ggcagggcct
    16201 tcgacgacaa cgaagctgcc tccaccgtcg cgggcccggt cgagcgcggc tgcgagcctc
    16261 gcgagttcgc catcacgttc gaggagcgct gagcccgtcg gatgtgccga gccactcatg
    16321 caggtaactg gtttgggtag ttcgaacccg ccatggcggg tacaagcgtc acacaaagtc
    16381 agggggagca ccacggtgcg agttgctacc ggcgccgtcg acactgccgg agtacaccgg
    16441 gagcgggaag tacccgctcc cggactggat cggaggtgcg tcatggccga gaagaggacc
    16501 accgcatacg gtcggagcgg gaagaagtgg ctggcgatct atgcgatcgt cggggctgtt
    16561 gcgtttttga tcgtatatct cgtgttcttc tccgagggcg gcggcaccgg agggctgtat
    16621 tagccggatc gccaacacgt gagcgacccg gtcggttatg tgtttcgcca gggagatgga
    16681 ggggttggcg ctcggtggat cgagcgccga tccccataca agctctcgtg agtcatgagg
    16741 atctgatccg gagcgttccc acgccttcag atcgatattt ctagggcctg tgacgaaccg
    16801 cccttcatgc ctcgattcaa tcggcgtctc gacggatcgg gggaagctcc ggccagccag
    16861 accgtgacgt gaggcggtct aggtctggaa ctgacggaca tcgtgtgcac atcgggctcc
    16921 cggatcgagg ggaggaccga tgaccgaacg agagctcgcc cgtgcggcgg cccacccgct
    16981 gacgatcatc cgccacgccc aggaggtaac cggcaacgtc tcgttgacct gccgctactt
    17041 cgggatcacg cgccaggcct accacatctg gctccgccgc tacgaggagc agggcctgga
    17101 ggggttccgg gaccgttcgc gaaggcctca cgtgagcccg aacgccacga gggccgaggt
    17161 cgtcggcaag atcgtctacc tgcgccagag ctatcacttc ggacctcaca agatcgcgat
    17221 gtatctcaag cgctaccacg acaggggtca gttcaggggt cggccccgac ttccgatcac
    17281 gcggcgaacc aattacaccg ggggctgccc gggctgactc ctcaagaggc acgggcaaca
    17341 gccctcctag gccggctcgc tgaacttctg cttctactcg acgaatggca tcgttgatcg
    17401 tgcgccgcca tcgccctacc ccgctcggtc ccgacgaagc cgcgaaaggg ggcgggagcg
    17461 gtggtggagt tcgctgcgag aggtccgttt ggggcgcctc gtccctaccc atccatgcca
    17521 agtcttcgcg ccgagaaaag cgtggaggtg gcttgggctg ggcatcgggc acctgaacaa
    17581 cggagcagcg aaccgcgtgt cattgctcga tacgatcggc ggcacgaaga cgggtgtcag
    17641 ctacgcggag cgggatggtc cacgggatgt cgcgtaccgg gcaccgaggt gatggaagcc
    17701 tccccccgcg acccagattc cgggcgaccg gcaacttaga ctcctggccg tgcccacctg
    17761 tccgaactgt ggggaggaca accccgagcg cgcccggttc tgcatgggtt gcaccgcccc
    17821 gctcgtcgcg gtggcgtccc gagggaaacg ccggaccgtc actatcatct tctccgacct
    17881 ggtggggtcc acggctctcg gagagcgtct tgacgcggag gcgctccgtg aggttctgga
    17941 tcgctacttc gaagaga
//
LOCUS       DASUYD010000114         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1381978, whole genome shotgun sequence.
ACCESSION   DASUYD010000114 DASUYD010000000
VERSION     DASUYD010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1381978"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="VF195_06245"
     CDS             <1..279
                     /locus_tag="VF195_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007917301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6580452.1"
                     /translation="PLEDAGWEDDEDHPRGPEEVVDAFELTWAMIDDCLQRWTPDDLA
                     VEFTRLRRTGTQTFTRQWVIWHLIEHDLHHGGEISLILGSHGLSGLDL"
     gene            700..1068
                     /locus_tag="VF195_06250"
     CDS             700..1068
                     /locus_tag="VF195_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580453.1"
                     /translation="MGTRALRAVVIAAALTAAVVGFLLLRPGDAEHQPQATIEQALAG
                     FVGDRFGVGYIGTCPLEFPADGDVPRGMCSARFSGADGRVLYRVGNPFSEWVGEATLV
                     RDASGSWHVASFEEYPPLGG"
     gene            complement(1178..1906)
                     /locus_tag="VF195_06255"
     CDS             complement(1178..1906)
                     /locus_tag="VF195_06255"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6580454.1"
                     /translation="MDRDRRTAEGLPEPPWRSGRARTAPRLPLTRDAIVEAALTVLER
                     EGMEGLSMRKVGEELGTGAASLYWHVRNKEELLQLLFERVTEEIDLPEPDPSRWQEQC
                     KELARQMLDVMRRHRDVARISLGRIPSGPSVARFSEWLFELLQPVGIPDRVIAYLGDL
                     MGLYIGAYAFEESLGLVSPTGEEMPPEEIIRMLRDYMLSLPADRFPRTLAAIDIMFDG
                     TTHERFEFALDLIVRGLETHASPT"
     gene            2030..>2230
                     /locus_tag="VF195_06260"
     CDS             2030..>2230
                     /locus_tag="VF195_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580455.1"
                     /translation="MKGTRSLETDAGASAGQSGAREGINRRPSGVPAGRRAPADRRSG
                     DPGAVRARLNPTEPAVAVKGGTI"
BASE COUNT          369 a          736 c          774 g          351 t
ORIGIN      
        1 ccgctcgagg acgcggggtg ggaggacgac gaggaccacc ctcgggggcc ggaggaggtc
       61 gtcgacgctt tcgagctgac ctgggcgatg atcgacgact gcctccagcg ctggacgccg
      121 gacgacctcg cggtcgagtt cacgcgcctg cgccgtacgg gcacccagac gttcacacga
      181 caatgggtga tctggcacct gatcgagcac gacctccacc acggcgggga gatctcgctc
      241 atcctcggga gccacggcct ctccggcctc gacctgtaac aagcctcgtc gatcccggca
      301 tcgatcgccc gagaccattt gtcgtcgacc cgaccgcgct ttgccaccgg gcgacctccg
      361 ctcaagcaac gaccgccggc aggcggcggg aggggtggga ttcgaaccca cgtacgggtc
      421 gccccgtaag cggttctaag accgattgag acacgtccgc aaccaggacg atgagacgaa
      481 agagcctgtg gactgcggtt tcgcgtctct tcgattcccc tcggttcccg tcgcttcccc
      541 gccaattgtg gccccgatgt ggccccactc cagcctttcg gagcgggtcg tcgtggctgc
      601 ttcggcccgt gcatcgacaa ttgcggccat cggggaccat ccgggaggcc gctcagcatc
      661 acaggaactc cgcagggcga tcgtgcgtct atgcggggcg tggggacacg agcactgcgc
      721 gcggtggtga tcgctgctgc cctcacggcg gcggtcgtgg gtttcctgct gctccgcccc
      781 ggcgacgccg aacaccagcc gcaggcgacg atcgagcagg ccctcgccgg gttcgtgggc
      841 gaccggttcg gcgtggggta catcggcacg tgccccctgg agttccccgc ggacggcgac
      901 gtccctcggg gcatgtgctc cgcgaggttc tccggcgcgg acggccgggt cctgtatcgc
      961 gtgggcaatc cgttcagtga gtgggtcggg gaggcgacgc tcgtccgcga tgcatctggg
     1021 tcctggcacg tggcgagctt cgaggagtat ccgcccctcg gcggctgaac atcccggccg
     1081 cgagctcgtt ggagcggggt ggtgaagaga gaacacatcc ccctcattgc gattcagcaa
     1141 gggatatccc gaggggccgc aggcgacaca gacgacccta cgtgggcgag gcgtgggtct
     1201 cgaggccgcg aacgatgaga tccagcgcga actcgaagcg ctcgtgcgtt gtgccgtcga
     1261 acatgatgtc gatggccgcg agcgtgcgcg ggaaccgatc cgcaggaagc gagagcatgt
     1321 agtcgcgaag catgcggatg atctcctccg gcggcatctc ctcgccggtc ggtgagacga
     1381 gcccgaggct ctcctcgaac gcgtaggccc cgatgtacag acccatcagg tcgccgaggt
     1441 aggcgatcac gcgatccggg atcccaacgg gctgaagcag ctcgaagagc cactcgctga
     1501 accgggccac gctgggaccc gaggggatcc tgccgagcga gatacgcgcc acgtcacgat
     1561 gccggcgcat cacgtcgagc atctgccgcg cgagttcctt gcactgctcc tgccagcggg
     1621 acggatctgg ctccgggagg tcgatctcct cggtgacgcg ctcgaacaac agttgaagga
     1681 gctcctcctt gttgcggacg tgccagtaga gcgaggccgc tccggtcccc agctcctcgc
     1741 cgaccttgcg catcgacagg ccctccatgc cttcgcgctc gaggacggtg agtgccgcct
     1801 cgacgatcgc gtcccgggtc aacgggagtc tcggcgcggt gcgggcccga cccgaacgcc
     1861 acgggggctc gggaaggccc tcggccgtcc gtcggtcacg gtccatggct ccaacagtgt
     1921 actacggtcg aacatagctc gggtcctcga acatcgtttg acaggatggg cctcaagagt
     1981 gcagaatgat gttccgttac ggaacgatgt tcgacccagg gaggccgaca tgaagggaac
     2041 gaggagcttg gagaccgacg cgggggcgtc ggccgggcag tcgggcgctc gcgaggggat
     2101 caaccgccgc ccgtctggag ttcccgcggg gcgccgcgcg cctgcggatc gccggagcgg
     2161 agatcccgga gctgttcggg cgcgcctaaa cccgaccgaa cccgccgtcg cggtgaaggg
     2221 cggaacgatc
//
LOCUS       DASUYD010000115        24714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1823989, whole genome shotgun sequence.
ACCESSION   DASUYD010000115 DASUYD010000000
VERSION     DASUYD010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1823989"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /gene="rplK"
                     /locus_tag="VF195_06265"
     CDS             <1..342
                     /gene="rplK"
                     /locus_tag="VF195_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012785138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEX6580456.1"
                     /translation="GQHGVNIMDFCKQYNEATQQFAGQVIPVELTIYEDRSFTFVLKQ
                     PPAAELIRKAAGIEKGSGEPNREKVARLSRQQVKEIAERKMVDLNANDIDNAMRIIEG
                     TARSMGVEVAS"
     gene            339..1061
                     /gene="rplA"
                     /locus_tag="VF195_06270"
     CDS             339..1061
                     /gene="rplA"
                     /locus_tag="VF195_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041546211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEX6580457.1"
                     /translation="MSAKPGKRYREAAESFDREQQYLPADAIKILKSLPGANFDETIE
                     VAFRLGVDPRKNDQAIRGTVALPHGTGKSVRVGVFAAGDKAREARDAGADVVGGEELV
                     EEVMKGTIDFDAAVATPDMMASVGKAGRVLGPRGLMPNPKTGTVTNDIAKAVADIKGG
                     KVEYRADRTGNVHLIIGKKSFEERNLLENYLVVIDELLKAKPSSAKGRYIKSLAVSST
                     MGPSVRIDASKPRGVQMAAAEG"
     gene            1234..1926
                     /locus_tag="VF195_06275"
     CDS             1234..1926
                     /locus_tag="VF195_06275"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580458.1"
                     /translation="MEQTTIGVLLVRNETLLFTDALAKAMGSDPSVRLISKPLSLDEA
                     LELCKEQRPGVVLLEASEMSEGSVSGSVRTLSQACGDAPVILLADSEVDDSFLVAGME
                     AGARGIVDGTARIAEVVGAVQAAAAGHRVVDQDRFVIAVEVTARLREGKRKRTELLGL
                     LSERESEILAELDRGLRNSDIAERLRISPRTVEKHVHNILAKLQVSSRLAAVALARET
                     GDPVYRSTRGTA"
     gene            2030..2533
                     /locus_tag="VF195_06280"
     CDS             2030..2533
                     /locus_tag="VF195_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020419483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580459.1"
                     /translation="MYQPKHARTTRRAPTPRFVVLTAVLVGLMAAPALAQSGHFVQTQ
                     TCRDIGTQVVCSGKVAGLGGTTFEITVAAEGIASVECTNPGGNVAPGQDTAVTVAGTT
                     EPLPTPRNGQFRYTITSDDPEPLPATPTCPNNQWTPNIVDVTFTTATLTLLEDGIVSD
                     TVTVPVS"
     gene            2798..3430
                     /gene="rplJ"
                     /locus_tag="VF195_06285"
     CDS             2798..3430
                     /gene="rplJ"
                     /locus_tag="VF195_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007167797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEX6580460.1"
                     /translation="MPSTQKIERVAELKEQIESSTALLLTEYRGLTVSEITELRKSLG
                     EGGARFAVIKNTLMRRALEGTDAAGLGSLFEGPSAVAFVLEDPVAAARSVTAAARKFP
                     ALVLKGGFVEGRLLSAEEARSLAELESREVMLSKIAGLMKGEMSRAASTFQAAQSRFL
                     SLLEAFKAKLPEAAPEPDAAPEPDAAPEAEAAPEAEAAPEANGTNEKEGE"
     gene            3433..3831
                     /gene="rplL"
                     /locus_tag="VF195_06290"
     CDS             3433..3831
                     /gene="rplL"
                     /locus_tag="VF195_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006130601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEX6580461.1"
                     /translation="MAKSKIGTEDLLEQFKEMTLLELSEFIKAFEETFEVTAAAAAPM
                     MVAAPGGGAGGDGGAEEEQSEFDVILTAAGDKKIQVIKEVRALTSLGLKEAKDLVDGA
                     PKPVLERVNKEAADKAKEQLEAAGGSVEVK"
     gene            3938..4354
                     /locus_tag="VF195_06295"
     CDS             3938..4354
                     /locus_tag="VF195_06295"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6580462.1"
                     /translation="MRESDGKHFGQRLRESRVAAGLSQSELEDISGIPKARLSRYENG
                     HVEPSIQTLARLSRALNVSEASLLGDQRAILEDFFNVLYDRGVRITSSEQGARIAHAI
                     ADLLGALKPDPVAADNVELTRVPDAVDHAVAAPMLG"
     gene            complement(4351..6126)
                     /locus_tag="VF195_06300"
     CDS             complement(4351..6126)
                     /locus_tag="VF195_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580463.1"
                     /translation="MAGLATFAALLVLDGIWAGRSLVRGLTAARSELSIAIESIVTGD
                     PGSAGQHFDAATRAADAARGAVGHPSMGIAGLLPIAGDNIDAAAAVADASRATAEAGT
                     AMVEVARTLGWTDIRIPASTATGSVDIGAFEDAIPDMEMVTGRLDDALRALEGAGGDG
                     LLGPVASGYRDAVDGLTLRLDLAIRFRDSLRLAAEMFEGEHRYLVCVPTLGIPRPAGG
                     TPATVGVLVARDGSLALESIAPAPSELIDAEISIDRQRTARALLEAAETSGLTDLDGV
                     IQIDAVALEDIVWAIGDVEAEGSHEALSDRTTARALEIEAFLGTAPPRSARLHADRVS
                     AILEAFLHRRPGVESFALATAAGARDGHLSIYLPGRAERRIIRSLGLDGGTRVTDDGV
                     LPVAASWSAAGNSHVGALVDTTVRQSIRIMGDGSAAVDAEILFENKAGTDPPSVLLGR
                     PVGGIPVGTFAADVTLFAPATAQDITAETSRPSPIDIGSDVGLTSVTGSILVRGGESA
                     TLTVTYEVPDAVRTVDGLEQLMLRVLPQPTLSGVRFQLQIVLPEGSNVVSTSRGLERR
                     TDGATFSGVRGGSVDLELRFGAGGP"
     gene            6517..9957
                     /locus_tag="VF195_06305"
     CDS             6517..9957
                     /locus_tag="VF195_06305"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX6580464.1"
                     /translation="MPVRNRVSFAKLDEVLQLPDLVSIQKDSFYRLLNEGLKEVLEEV
                     SPIEDFTEQFQLYFGKHEFRAMKFSEEECRDKDITFSAPLFVEATFVNKVTGEIKEQE
                     VFLGDFPMMTDRGTFIINGTERVVVSQLVRSPGVYFSKEPDKTTDKDIFIAKIIPSRG
                     AWLEFDVDKKDTVGVRIDRKRRQHITVFLKALGFSAAEILKLFDNAVSIQNTLEKDHF
                     ETQEEALEDIYRKLRPGEPPTAESAKTLLENLFFNPKRYDVARVGRHKVDKKLGAAEG
                     ELLKQLRSHHKGLGELDNPDRKAWEQIRYRVFSSMQKEEAAGQGAPKYKAVLTFEDIL
                     KTVGYLVHLHAGEAGYDPDDIDHFGNRRIRSVGELIQNQIRIGLSRMERVVKERMTTQ
                     DVEAITPQTLINIRPVVASIKEFFGTSQLSQFMDQTNTLAGLTHKRRLSALGPGGLSR
                     ERAGFEVRDVHPSHYGRMCPIETPEGPNIGLMGSLSSFGRINEFGFIETPYRRVEKGK
                     VTAKIDYLSADEEDRHVIAQANARLAPDGTFQDTQVLVRRKGGEVDQVSPDEIDYMDV
                     SPKQLVSVAAALIPFLEHDDANRALMGANMQRQAVPLLRAEAPLVGTGVEFRAAVDAG
                     DVVLAEEAGTVEDVSAEAIVVREKTGKLRTYDLIKFRRTNQGTNFNQKPLVDIGDRVA
                     KQQVIADGPSTDQGEIALGKNLLVAYMPWEGHNYEDAIIISERLVKDDILTSIHIEEH
                     EVDARDTKLGAEEITRDIPNVSEEILKDLDERGIVRIGAEVNTGDYLVGKVTPKGETE
                     LTPEERLLRAIFGEKAREVRDTSLKVPHGESGKVIGVRVFNRDDGDELPPGVNQLVRV
                     YVAQKRKISDGDKLAGRHGNKGVISTILPEEDMPFLEDGTPVDIILNPLGVPSRMNLG
                     QVLEAHLGYAALVGFDKSGEDHTGPVHVATPVFDGAKEDEILAAIRSSHAASDGKKLI
                     NDIGKARLYDGRNGQPYAEPVTVGVAYILKLLHLVDDKIHARSTGPYSMITQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVLGRVKVYEAIVKGENIPEPGIP
                     ESFKVLIKEMQSLSLNVEVRSSSGEEIELRETDEDVFRAAEELGIDLSRREPAGADFD
                     "
     gene            10079..13999
                     /locus_tag="VF195_06310"
     CDS             10079..13999
                     /locus_tag="VF195_06310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX6580465.1"
                     /translation="MLDVNYFDELRIGLAAADQIRAWSNGEVKKPETINYRTLKPEKD
                     GLFCERIFGPTRDWECHCGKYKRVRFKGIICERCGVEITRSKVRRDRMGHIELAAPVT
                     HIWYFKGVPSRLGYLLDIAPKDLERIIYFAAYVVTRVDDERRHKDLPEIEEEIEGEKR
                     EIEAERDDQVKRRLSELEDRLKELEAEGAKGAQLSAAKREAQRDVERIHEAAGRDTQH
                     LDDVVSAFKSLKIKQLVADDAVYRSLRERFADYFDGGMGAEAIKRLLQDYDVVAEGEA
                     LRETIENGKGTKRLKAIKRLKVASNFVEGRSSPMGMVLDAIPVIPPDLRPMVQLDGGR
                     FATSDLNDLYRRVINRNNRLKRLLDLQAPEIIVNNEKRMLQEAVDALFDNGRRGRPVT
                     GPGNRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKQMALE
                     LFKPFVMKRLVDHNLAQNIKSAKRMVERARPQVWDVLEEVIREHPVMLNRAPTLHRLG
                     IQAFEPVLVEGKAIKIHPLVCAAFNADFDGDQMAVHVPLSAEAQAEARILMLSTNNIL
                     SPAHGRPIATPSQDMVLGLYYLTLTQNEDMKDEEIPTFGSTAEALLALDRGHPLHDPV
                     RVRLTGQALPEELLPEGYDPKADGWQGRTPVIRTTIGRIVFNTAFPDDFEFRNEPVLK
                     PDVARIVDEVVHRYDRAQVEIVLDNLKNLGFDYATKAGVTIGVEDVTTPPEKQKILDE
                     HEKRAQKVEQQYRKGIITDDERRQELIEIWTQATDEVKDAMEAQFTKTNPIFMMANSG
                     ARGNIMQIRQLAGMRGLVANPKGEIIPRPIKSNFREGLSVLEYFISTHGARKGLADTA
                     LRTADSGYLTRRLVDVAQEVIVREEDCGTDRSIGAVVNVTRPDGTLVKGAHTDTALFG
                     RVLAEDVKVGGKKIAARGAELSDELAEAVIDSGVETVQVRSVTTCEAEIGVCQACYGR
                     SLASGRLVDIGEAVGIIAAQSIGEPGTQLTMRTFHTGGIAGEDITHGLPRVVELFEAR
                     NPKGMAQITELSGEVAIEDDDEKHVRRIRVTDETGDMVEYQVSFRARLTVSNGDMVEV
                     GQQLTEGSVNPHEKLRVEGVLALQMHLVDEVQQVYRSQGVTIHDKHIELIARQMLRKV
                     HIIEPGDSDLLPGDLVNRRVFEETNQEVVESGGVPASARPVLLGITKASLATDSWLSA
                     ASFQETTRVLTDAAIQSKADPLLGLKENVIIGKLIPAGTGMSRYRNVAVKIKPDAIPE
                     YWLTRQRELAELAELAEGGEPALVGLTRQQAESMLGGVARSDEE"
     gene            14308..14682
                     /gene="rpsL"
                     /locus_tag="VF195_06315"
     CDS             14308..14682
                     /gene="rpsL"
                     /locus_tag="VF195_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEX6580466.1"
                     /translation="MPTIQQLVRKGRARPKKRTKSPALKGSSQRRGVCTRVYTMTPKK
                     PNSALRKVARVRLTSGIEVTVYIPGVGHNLQEHSIVLVRGGRVRDLPGVRYKIIRGTL
                     DTAGVRDRRKARSRYGAKKVGE"
     gene            14684..15154
                     /gene="rpsG"
                     /locus_tag="VF195_06320"
     CDS             14684..15154
                     /gene="rpsG"
                     /locus_tag="VF195_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX6580467.1"
                     /translation="MPRKGPAVRRGITPDPVYQNPLVTQLVNKVLLHGKKTVAEHIVY
                     EALEQISQKTANDPVITLKRAVDNVRPMLEVKSRRVGGASYQVPVEVKPTRGTTLALR
                     WLVNFSRARRENSMAERLVAEIMDASNGQGASVKRREDMHKMAEANRAFAHYRW"
     gene            15216..17372
                     /gene="fusA"
                     /locus_tag="VF195_06325"
     CDS             15216..17372
                     /gene="fusA"
                     /locus_tag="VF195_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6580468.1"
                     /translation="MAVPAERMQVSQTEQKVKDGKGLSIREYPLARTRNIGIMAHIDA
                     GKTTTTERILYYTGKAYKIGEVHEGTAQMDWMVQERERGITITSAATTCQWKGHWINI
                     IDTPGHVDFTVEVERSLRVLDGAVAVFDAVAGVEPQTETVWRQADRYKVPRICFINKM
                     DRTGANFERTVDMIVDRLQATPLILQLPWGTESDFHGVIDLVQMNAHYWEGDMGEEWH
                     DTEIPEEYRAAAEAAHHVLFERLADHDESLMEKFVHEEEPTIEEMHRAIRRATLASLG
                     VPVMCGSAFKNKGVQPLLDAIVAYLPSPAEVLPVEGHLPNDESTHVQRAPEDTEPFAA
                     LAFKIMSDAFVGRLTYLRVYSGVLRAGSHVANATKDRKERIGRILQMHANHREDMAAA
                     YTGDIVAVVGLKHATTGDTICDPEHQVVLESISFPTPVISVAVEPKTKADQDKLGTGL
                     AKLADEDPTFVVKFDDETGQTVISGMGELHLDIIVDRLKREFSVDANVGKPQVAYRET
                     IRRQVHKVETRFVRQTGGRGQFAHVVIDLEPTGAGGGYEFLNKIVGGKIPREYIPAVD
                     QGIQEALDNGVLAGYPMVDVRATLMDGTYHEVDSSEMAFKIAGSMALKEAARKADPAL
                     LEPVMEFEVTTPEEFMGEVMGDVTARRGRIERIDDAGGQKVIRVLVPLAELFGYATDL
                     RSRTQGRAAHSPMQLHAYAEVPVQVSKEIIARVSGE"
     gene            17422..18606
                     /gene="tuf"
                     /locus_tag="VF195_06330"
     CDS             17422..18606
                     /gene="tuf"
                     /locus_tag="VF195_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEX6580469.1"
                     /translation="MGKKKFERTKPHVNIGTMGHIDHGKTTLTAAITKRQAEKGMAEF
                     TPFDEIDKAPEERERGITIAIAHVEYETDNRHYAHVDMPGHADYVKNMITGAAQVDGA
                     ILVVSAADGPMPQTREHVLLARQVGVPSIVVALNKCDMVDDSELLDLVELEVRELLSS
                     YEYPGDDIPVVQISALKALEGDETWMAKVDELLEACDTYIPEPVRDTDKPFLMPIEDV
                     FSITGRGTVVTGRVEQGVLKKMGEVEIVGLRDTQKTTATDLEMFRKLLDEITAGDNVG
                     VLLRGIDKDQVERGQVLAKPGSITPHTEFMSNVYILSKEEGGRHTPFFDGYKPQFYFR
                     TTDVTGVAHLPEGVEMVMPGDNVEMRVELDKPIAMEEGLRFAIREGGRTVGAGRVTTV
                     LK"
     gene            18674..18982
                     /gene="rpsJ"
                     /locus_tag="VF195_06335"
     CDS             18674..18982
                     /gene="rpsJ"
                     /locus_tag="VF195_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEX6580470.1"
                     /translation="MAGPKIRIKLRAYDHRMLDVAARDIVEHVTRTGAKVVGPVPLPT
                     ERSIYTVIRSPHKYKDSREHFQMLTHKRLIDIVDATSKTVQELQRLNLSAGVDIEIKL
                     "
     gene            18988..19641
                     /gene="rplC"
                     /locus_tag="VF195_06340"
     CDS             18988..19641
                     /gene="rplC"
                     /locus_tag="VF195_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX6580471.1"
                     /translation="MAETKGILGTKLGMTQIFDDTRAVPVTVIKAGPSVVSQVKTKET
                     DGYDAVQLSFGTVRPRDLTRPMKGHFESHDATPGRHVVELRTDDAGSYEPGQEIRVDV
                     FSPGDRVDVVGVSKGHGTTGVMKRHGFHGLRASHGTKRKHRAPGAIGACATPSRVFKD
                     MPMAGQHGSVRVTVLNLEVVQADVERGLLLVKGAVPGPNGGLLMARSAVKAPAKGGA"
     gene            19641..20327
                     /gene="rplD"
                     /locus_tag="VF195_06345"
     CDS             19641..20327
                     /gene="rplD"
                     /locus_tag="VF195_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006375997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEX6580472.1"
                     /translation="MASIEKRDAAGKKAGSVELPAEIFESKVSVPLMHQVVVAGLAGI
                     RAGTHSTKTRAQVRGGGRKPWRQKGTGRARQGSIRSPQWVGGGVAHGPTPRDHSQKIN
                     KKMRRGALRSALTDALQSGKLAVVDDLDWSEPKTKQAVGVLASLELAGRILLVLPQPT
                     ESGNVEKSFRNLPNVKIAYSGGLGTYDVLLADRVLFTDAAIDRLTEPPAGSPTGASEG
                     SAGQVEEVAG"
     gene            20324..20620
                     /gene="rplW"
                     /locus_tag="VF195_06350"
     CDS             20324..20620
                     /gene="rplW"
                     /locus_tag="VF195_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010145317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEX6580473.1"
                     /translation="MNKSPRDVIVRPIVSEKSYAGIEHNTYTFLVDPRANKTEIKEAV
                     QTIWDVQVLSVNTLKRRGKMKRRGYTRGRRPDEKRAVVTLAQGDSIEIFEGGGS"
     gene            20623..21453
                     /gene="rplB"
                     /locus_tag="VF195_06355"
     CDS             20623..21453
                     /gene="rplB"
                     /locus_tag="VF195_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX6580474.1"
                     /translation="MAVRKYKPTTPGRRGGSVSSFDEITRSTPEKSLLTKGRKKAGRN
                     VHGRITTRHQGGGTKRRYRLIDFKRNKDGIPAKVATIEYDPNRSSRIALLHYADGEKR
                     YILAPDGVRVGDQLLSGEGAEIRPGNALPLRNIPVGTVVHAVELKPGAGARMARSAGT
                     SVQLMAKEGKLATLRLPSGEMRTVPSICKGTVGVVGNAEHELIAIGKAGRNRWKGKRP
                     SVRGVAMNPVDHPLGGGEGKASGGRHPTSPWGKKEGRTRKKKKASNAQIIRRRGKGRG
                     "
     gene            21453..21734
                     /gene="rpsS"
                     /locus_tag="VF195_06360"
     CDS             21453..21734
                     /gene="rpsS"
                     /locus_tag="VF195_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX6580475.1"
                     /translation="MARSVKKGPFVDEHLMVKIAGMNERSEKKVVKTWSRRSTIVPDM
                     IGHTIAVHDGRKHVPVFISESMVGHKLGEFAPTRTFRTHSRDERRTGPR"
     gene            21735..22079
                     /gene="rplV"
                     /locus_tag="VF195_06365"
     CDS             21735..22079
                     /gene="rplV"
                     /locus_tag="VF195_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX6580476.1"
                     /translation="MEAKATVKYVRTSPRKMRRTVDLIRGQHVGEAKRILRLSTLGAS
                     RDVEKLLDSAIANAEQRPGVIAENLLVSKAWVDEGPTLRRFRPRAYGRATRVRKRTSH
                     VTVVVETMGDEE"
     gene            22079..23032
                     /gene="rpsC"
                     /locus_tag="VF195_06370"
     CDS             22079..23032
                     /gene="rpsC"
                     /locus_tag="VF195_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011435045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX6580477.1"
                     /translation="MGQKVHPYGFRLGVIYPWKSNWYANRRDYIEALHEDVWIRKHIR
                     SRLSRAGISSIDIERKGDQIWVYIRTARPGIVIGRKGAEVDRLRKDVERYTKKRVDVK
                     VEDMNQASSEVRPDTDAALLAQGVAEQLAGRVAFRRAMRRAVQTAMRAGALGVRVACA
                     GRLGGTDMGRKEWYREGRVPLHTLRAKVDFGTAAAKTTFGAIGVKVWVYHGDEVPHRE
                     QESERALARAHARAERGERGARPAATRGKAPAKAEPVAATPDAVAPVEEPSPTPPESG
                     PEPASVTPEATDDAEPAAAPEAAPVAEAPVEAPDTQEVGEG"
     gene            23032..23445
                     /gene="rplP"
                     /locus_tag="VF195_06375"
     CDS             23032..23445
                     /gene="rplP"
                     /locus_tag="VF195_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX6580478.1"
                     /translation="MLAPKRIKHRKQHRGRMRGLAKGHTDIQSGDYAIVAMEPGWITN
                     RQIEAARVAITRHIRRGGKVWITIFPDKPVTKKPAETRMGSGKGNPEGWVAVVKPGRV
                     MFELAGVNEALAREAMGRAQHKLPIRTKFITRVGE"
     gene            23448..23708
                     /gene="rpmC"
                     /locus_tag="VF195_06380"
     CDS             23448..23708
                     /gene="rpmC"
                     /locus_tag="VF195_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010228477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX6580479.1"
                     /translation="MASKTAELRELTDAQLLERAESAKEELFNLRFQLATGQLDDSSS
                     IKKVRHEIARIATVMRQRDIEAAATDAEAGAAGTESGTESER"
     gene            23705..23983
                     /gene="rpsQ"
                     /locus_tag="VF195_06385"
     CDS             23705..23983
                     /gene="rpsQ"
                     /locus_tag="VF195_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019663552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX6580480.1"
                     /translation="MSEEQQVTQERGRRKTRTGVVVSDGMDKTVVVRIDRSFRHPLYD
                     KTVRRSKKLAAHDESNEARVGDRVRVVETRPLSKSKHWRLVEIVEKAK"
     gene            23980..24351
                     /gene="rplN"
                     /locus_tag="VF195_06390"
     CDS             23980..24351
                     /gene="rplN"
                     /locus_tag="VF195_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX6580481.1"
                     /translation="MIQQETRCRVADNTGAKELLVIRVLGGSARRYASIGDTVVGTIK
                     DALPGGGVKKGEVVKAVVVRTSKERRRPDGSYIRFDDNAVVLLTPDSRNPRGTRIFGP
                     VARELRDRRYMKIVSLAPEVL"
     gene            24369..24710
                     /gene="rplX"
                     /locus_tag="VF195_06395"
     CDS             24369..24710
                     /gene="rplX"
                     /locus_tag="VF195_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEX6580482.1"
                     /translation="MPGVDIKKDDTVRVISGKDRGREGRVVRVLPREGRVMVDGVALA
                     KKHQRSSGRRARGSSQQLQQGGIIDAEMFVNISNVQVVCTACGEATRIGHRVEGDRKV
                     RFCRKCETELP"
BASE COUNT         4532 a         8045 c         8669 g         3468 t
ORIGIN      
        1 ggtcagcacg gcgtgaacat catggacttc tgcaagcagt acaacgaagc gacccagcag
       61 ttcgccggac aggtgatccc ggtcgagctc acgatctacg aggatcgatc gttcacgttc
      121 gtcctcaagc agccgccggc cgcggagctg atccgcaagg cggccgggat cgagaagggg
      181 tcgggcgagc cgaaccgcga gaaggtcgcc cgactctccc ggcagcaggt gaaggagatc
      241 gccgagcgga agatggtcga cctgaacgcg aacgacatcg acaacgcgat gcggatcatc
      301 gaggggacgg cacgcagcat gggcgtggag gtggcttcgt gagcgccaag ccagggaagc
      361 gctaccgaga ggcggccgag tccttcgatc gtgagcagca gtacctcccg gcggacgcga
      421 tcaagatcct gaagtcgctg ccgggagcga acttcgacga gaccatcgag gtcgccttcc
      481 ggctcggcgt cgaccctcgg aagaacgatc aggcgatccg cggcaccgtg gcactgcccc
      541 acggcacggg caaatcggtg cgcgtcggcg tgttcgcggc gggggacaag gctcgcgagg
      601 cgagggatgc cggagcggac gtcgtcggcg gcgaggagct ggtcgaggag gtcatgaagg
      661 gaacgatcga cttcgacgcg gcggtcgcca caccggacat gatggcgtcg gtcgggaagg
      721 ccggccgggt cttgggtccg cggggactga tgccgaaccc gaagaccggg accgtgacga
      781 acgacatcgc gaaggcagtc gccgacatca agggaggcaa ggtcgagtac cgagccgacc
      841 gcacgggcaa cgtccatctg atcatcggga agaagtcgtt cgaggagcgc aacctgctcg
      901 agaactacct cgtggtcatc gacgaactcc tcaaggcgaa gccatcctcg gccaagggcc
      961 gctacatcaa gtcgctggcc gtctcctcga cgatggggcc gagcgtgcgg atcgacgcgt
     1021 ccaagccccg aggcgtccag atggcggcgg cggagggata gtccgagaag cgcccggcaa
     1081 cagcgccgca caacgattgc ggcgcggtcc atctacccgg gaggcgggcc agcacacgac
     1141 gtattcactt gtcgtgatgc ctggtctaca cttcgtcccc caatcagtcc aggccatcta
     1201 ggggacaagc cctccacggg ggcgaggtgg gagatggagc agacgacgat cggcgtgctg
     1261 ctggtccgga acgaaaccct gctcttcacc gatgcgctcg cgaaggcgat gggctcggat
     1321 ccttcggttc gtctgatctc caagccgttg tcgctggacg aagccctcga gctctgcaag
     1381 gaacaacgtc ccggggtcgt gttgctcgag gcctccgaga tgtccgaggg atcggtgagc
     1441 ggatccgtgc ggaccctctc ccaggcctgc ggcgatgccc ctgtgatcct gctggccgat
     1501 tcggaggtcg atgactcctt cctggtggcg gggatggagg cgggagcccg cgggatcgtg
     1561 gacggcacgg cccggatcgc ggaggtcgtc ggagccgtcc aggccgccgc cgccggtcat
     1621 cgtgtcgtgg atcaggatcg tttcgtcatc gcggtggagg tgacggcgcg gctccgcgag
     1681 gggaagcgca agcgcaccga actcctgggg ctcctgtccg agcgtgagag cgagatactt
     1741 gcggagctcg atcgcgggct ccggaactcc gacatcgccg aacggctcag gatcagcccg
     1801 cggaccgtcg agaagcacgt ccacaacatc ctggcgaagc tccaggtgtc ctcgcgcctc
     1861 gccgccgtcg cgctcgcccg agagacgggc gatcccgtgt acagatcgac gcgtggaacc
     1921 gcgtaggcgg aattgcgcgg tcgcgccgat gccgtggctt tcccgccgga cctagcctga
     1981 gaagcgctgg gtccgaccgc ggaggtaccg cggggaaggg aggtcccaca tgtatcaacc
     2041 gaagcatgcg aggacgactc gcagggcgcc cacaccgagg ttcgtcgtgc tgactgctgt
     2101 gcttgtcggc ttgatggcgg ctccggcgct cgcgcagagc gggcatttcg tgcagacgca
     2161 gacctgccgt gatatcggaa cgcaggtggt gtgctccggc aaggtggcgg ggctcggggg
     2221 gacgaccttc gagatcaccg tcgcggcgga gggcatcgcg tcagtggagt gcacgaaccc
     2281 cggcgggaac gtcgctccgg gccaggacac ggcggtcacc gtggcaggga cgaccgagcc
     2341 gctgccgacg ccgcggaacg gtcagttccg atacaccatc acgagtgacg atcctgagcc
     2401 gctcccggcg acgcccacct gtccgaacaa ccagtggacg ccgaacatcg tggacgtgac
     2461 gttcacgacg gcgaccctga cgttgctcga ggacggcatc gtgtcggaca cggtcaccgt
     2521 gcccgtcagc tagcgcgcaa ctcggatcac gcgaaggggc cgggctggcg ctcggcccct
     2581 tctgcgcttc ttcacagctg gatcgacagg acacctgcgg caactgctgt atcctgaccc
     2641 ggctgcagac agcaggggcg cgtgagcgca caatcccgca tcgggtacct gccgaggcga
     2701 cgatcttgcg tggcggacac gcatcgtcgt cggggaaggt cgcgccgcgc gcgtctccgc
     2761 tgtctgcggg ggcgcgttgt tcattacagg aggcgcgatg ccgagtaccc agaagatcga
     2821 acgggtcgcg gagctgaagg agcagatcga gagctcgacg gcgctgctgc tcaccgaata
     2881 ccgcggactg accgtgtccg agatcaccga gctgcgcaag tccctcggcg aaggaggcgc
     2941 gcggttcgcg gtcatcaaga acaccctgat gcgccgggcg ctcgagggca ccgacgccgc
     3001 cgggctcgga tcgttgttcg aagggccgag cgccgtggcc ttcgtgctcg aggatccggt
     3061 cgcggcagcc agaagcgtta cagcggcggc gaggaagttc cctgccctcg tgttgaaggg
     3121 cggattcgtg gaggggcgtc tcctctcggc cgaggaggcc agatcgctcg ccgagctcga
     3181 gtcccgcgag gtgatgctgt ccaagatcgc ggggttgatg aagggcgaga tgtcccgcgc
     3241 ggcttcgacg ttccaggcgg cccagtcgcg cttcctgtca ctgctcgaag ccttcaaggc
     3301 caagctgcct gaagcagcac cagagcccga cgcagcacca gagcccgacg cagcaccaga
     3361 ggccgaagca gcaccagagg ccgaagcagc acccgaggcg aacggaacca acgagaagga
     3421 aggggagtga ccgtggcgaa gtcgaagatc gggacggagg atctcctcga gcagttcaaa
     3481 gagatgacgc tgctcgagct ttcggagttc atcaaggcgt tcgaagagac gttcgaggtc
     3541 acggccgctg ccgccgcgcc gatgatggtg gccgctccag ggggtggagc cggcggtgac
     3601 ggtggcgcgg aagaggagca gtcggagttc gacgtgatcc tcacggctgc cggggacaag
     3661 aagatccagg tgatcaagga agtccgggct ctcacgagtc tcggtctcaa ggaggccaag
     3721 gatctcgtcg acggagcgcc caaaccggtc cttgagcgtg tcaacaagga ggccgccgac
     3781 aaggcgaagg agcagctcga ggccgccggg gggagcgtcg aggtcaagta ggtgacccga
     3841 cgagcaacct gtggatcgcg cgacggcggc ccgggttccc ctcccggcaa cccttgtgct
     3901 agcgtgcggg aacgttccac atgaggggga gacgggcatg cgcgaaagcg atgggaagca
     3961 tttcggtcag cgactccgtg agtcgcgggt ggcagcgggg ctgagccaat cggagctgga
     4021 ggacatctcc ggcatcccca aggcccgact ctcccgctac gagaacggtc acgtcgagcc
     4081 ttccatacag accctcgccc ggttatcgcg agctctgaac gtctccgagg cctcattgct
     4141 gggcgatcag cgtgcgatcc tcgaggactt cttcaacgtc ctctacgacc gtggcgtgcg
     4201 catcacgtcc agcgagcagg gggcgcggat cgcccacgcc atcgcggacc tgctcggtgc
     4261 tctgaagccc gacccggtcg ccgcggataa cgtggagctg actcgggtgc cagacgcggt
     4321 cgatcacgct gtcgccgccc cgatgctcgg ctagggcccg ccggcgccga atcgcagctc
     4381 gagatcgacc gagcctcccc ggacgccgga gaacgtcgcc ccgtccgtcc ttctctcgag
     4441 ccctcgtgag gtggagacga cgttcgaccc ctccgggagg acgatctgga gctggaaccg
     4501 cacgccggac agcgtcggct gcgggagaac cctgagcatc agctgttcca gcccgtcgac
     4561 ggtcctgacc gcgtccggga cctcgtacgt gaccgtgagc gtcgccgact cgccaccgcg
     4621 aacgaggatc gaaccggtca ccgaggtgag ccccacgtcc gagccgatat cgatcgggct
     4681 cggtcgactc gtctccgccg tgatgtcctg cgccgtcgcc ggcgcgaaga gggtgacatc
     4741 ggccgcgaac gtgcccaccg ggatcccgcc cacggggcgc ccgaggagga ccgacggcgg
     4801 atccgtcccc gccttgttct cgaacaggat ctccgcgtcg accgccgcgg acccgtcgcc
     4861 catgatccgg atcgactgac ggacggtcgt gtccacgagt gcaccaacgt gggagttccc
     4921 ggccgcgctc caggaggcgg cgacggggag cacgccgtca tccgtcaccc tggtcccgcc
     4981 gtcgagtccg aggctgcgga tgatgcgacg ctcggccctc ccgggaaggt agatcgaaag
     5041 gtgcccgtcg cgagcgccgg cggccgtcgc gagggcgaag gattccacgc ctggccgacg
     5101 atggaggaac gcctcgagga tcgccgagac ccgatcggcg tgcaacctgg ccgagcgggg
     5161 cggtgcggtc cccaggaagg cctcgatctc cagtgccctg gcggtggtcc ggtcggaaag
     5221 ggcctcgtgc gaaccctccg cttccacgtc cccgatggcc cacacgatgt cctcgagcgc
     5281 caccgcgtcg atctggatga ccccgtcgag atcggtcaga ccggacgtct cggccgcctc
     5341 gagcagggct cgcgccgtcc tctgccgatc gatggagatc tccgcgtcga tcaactcact
     5401 cggtgcgggg gcgatcgact cgagtgcgag ggagccgtcg cgtgcgacca ggacgccgac
     5461 cgtcgcgggc gtgccgccgg cggggcgtgg gatgcccagc gtcgggacgc agacgaggta
     5521 ccgatgctcc ccctcgaaca tctcggccgc gagccgcaac gagtcccgga atcggatcgc
     5581 gaggtcgagc cggagggtga gcccgtcgac cgcgtcccga tagccggagg cgacgggccc
     5641 gagcagtccg tcgccccccg cgccctccag cgcccggaga gcgtcgtcga gccgtcccgt
     5701 caccatctcc atgtcgggga tcgcgtcctc gaaggcgccg atgtccacgc tccccgtggc
     5761 ggtagaggcg gggatcctga tgtcggtcca cccgagcgtc cgagcgacct cgaccatcgc
     5821 tgtgccggcc tcggccgtcg cccgggaggc gtcggcgacg gccgctgccg cgtcgatgtt
     5881 gtcgcccgcg atgggcagga gcccggcgat gcccatcgag gggtgcccca ccgccccccg
     5941 ggcggcatcc gccgcccggg tggcggcgtc gaagtgttgc cccgccgacc ccgggtcccc
     6001 cgtgacgatc gattcgatcg cgatcgagag ctccgaccgt gcggccgtca gcccgcggac
     6061 gaggctccgt cccgcccaga tgccgtccag gacgaggagg gcggcgaacg tggcaaggcc
     6121 cgccaaggcg ccgaggacaa cccgtctccc gcgggtcctt ggtcgccggg ggccggacgg
     6181 ggcgggctcc gaggccatcg ccgccgaccc taccgccggc gggcgcggga caccatcagg
     6241 tccgttcggc aggtccgctg gcaggtgctc gtggtgctct cgggctgccc ggctgcccgg
     6301 ctggcggggt cggatggcat ccagcgaagg ccgttcggcg gcttgaccct cgggctcgag
     6361 gaggcgtacc ctcgggggca gccgccgtgt ccggggcagg cgccgttgac ggtgcctgtg
     6421 cctcgtcgta gactgctcgt ttgccgtgcc gtccaccgcg ctctgccgtg cgctctcgac
     6481 gtctcccggg aggggttccg cttgcctgac cagctcgtgc ctgttcggaa tcgtgtcagt
     6541 ttcgccaagc tcgacgaggt cctccagctt cccgacctcg tttccatcca gaaggactcc
     6601 ttctatcggc tcctgaacga gggcctcaag gaggttctcg aagaagtctc acctatcgag
     6661 gacttcacag agcagttcca gctctatttc ggcaagcacg aattccgagc gatgaagttc
     6721 tcggaagagg aatgccgcga caaggacatc acgttctccg ctcctctgtt cgtcgaggca
     6781 accttcgtca acaaggtcac cggtgagatc aaggagcagg aggtcttcct gggcgacttc
     6841 cccatgatga ccgaccgcgg caccttcatc atcaacggca ccgagcgcgt cgtggtctcc
     6901 cagctggtgc gctcgccggg cgtctacttc agcaaagagc ccgacaagac caccgacaag
     6961 gacatcttta tcgcgaagat catcccgtcg cggggtgcat ggctcgagtt cgacgtggac
     7021 aagaaggaca ccgtcggcgt gcggatcgat cgcaagcgcc ggcagcacat caccgtcttc
     7081 ttgaaggccc tcggcttcag tgcggccgag atcctgaagc tcttcgacaa cgcggtctcc
     7141 atccagaaca ccctcgagaa ggaccacttc gagacccagg aggaggccct cgaggacatc
     7201 taccggaagc tgcggcccgg ggagccgcct acggccgaaa gcgcgaagac cctcctcgag
     7261 aacctgttct tcaacccgaa gcgctacgac gtcgctcgcg tgggtcgcca caaggtggac
     7321 aagaagctgg gtgccgccga gggcgagctg ttgaagcagc tgcgctccca tcacaagggc
     7381 ctgggcgagc tcgacaaccc ggaccggaag gcgtgggagc agatccgcta ccgggtcttc
     7441 tcctcgatgc agaaggagga ggcggccgga cagggggcgc ccaagtacaa ggctgtcctc
     7501 acgttcgagg acatcctcaa gacggtcggc tacctcgtgc acctgcacgc cggcgaggcc
     7561 ggctacgacc cggacgacat cgaccacttc ggtaaccgcc ggatccgctc ggtcggggag
     7621 ctgatccaga accagatccg gatcgggctc tcgcgcatgg agcgggtcgt gaaggagcgg
     7681 atgaccaccc aggacgtcga ggccatcacg ccgcagaccc tgatcaacat ccgtcccgtc
     7741 gtcgcctcca tcaaggagtt cttcgggacc tcgcagctct cgcagttcat ggaccagacc
     7801 aacacgctcg ccggtctcac ccacaagcgc cggctgtcgg cgctcgggcc gggaggtctg
     7861 tcgcgggagc gcgccggctt cgaggtccga gacgtgcacc cgtcccacta cggccggatg
     7921 tgcccgatcg agacgccgga gggtccgaac atcggtctca tggggtcgct ctccagcttc
     7981 ggccggatca acgagttcgg cttcatcgag accccgtacc ggcgcgtcga gaagggcaag
     8041 gtcacggcga agatcgacta cctgtcggcc gatgaggagg accgccacgt catcgcgcag
     8101 gcgaacgccc gcctcgctcc cgacggcacc ttccaggaca cgcaggtgct ggtgcgccgc
     8161 aagggcggcg aggtcgacca ggtctcgccc gacgagatcg actacatgga cgtgtccccg
     8221 aagcagctgg tctcggtggc cgctgccctg atcccgttcc tcgagcacga cgacgccaac
     8281 cgcgccctga tgggcgccaa catgcagcgc caggccgtcc ctctgctccg cgccgaggcg
     8341 ccgctggtgg gtaccggcgt tgagttccgc gcggccgtcg acgcgggcga cgtcgtgctg
     8401 gccgaggagg cagggaccgt cgaggacgtc tcggccgagg ccatcgtggt cagggagaag
     8461 actgggaagc tgcgaaccta cgacctgatc aagttccggc ggaccaacca gggaacgaac
     8521 ttcaaccaga agcccctcgt ggacatcggc gaccgggtcg cgaagcagca ggtgatcgct
     8581 gacggtccgt ccacggatca gggcgagatc gccctcggca agaacctcct cgtggcgtac
     8641 atgccctggg agggccacaa ctacgaggac gcgatcatca tctccgagcg cctggtgaag
     8701 gacgacatcc tcacctcgat ccatatcgag gagcacgagg tcgacgcccg cgacaccaag
     8761 ttgggcgccg aggagatcac gcgcgacatc cccaacgtct ccgaggagat cctgaaggac
     8821 ctcgacgagc gggggatcgt ccggatcggg gccgaggtca acaccggcga ctacctcgtc
     8881 gggaaggtca cgccgaaggg cgagacggag ctcacccccg aggagcggct gctccgagcg
     8941 atcttcggag agaaggcgcg cgaggtgcgc gacacgtccc tcaaagtgcc gcacggcgaa
     9001 tcgggcaagg tgatcggggt gcgcgtcttc aaccgggacg acggtgacga gctgccgccc
     9061 ggtgtgaacc agctcgtgcg cgtctacgtg gcgcagaagc gcaagatctc cgacggcgac
     9121 aagctggccg gacgccacgg gaacaagggc gtgatctcca cgatcctccc cgaggaggac
     9181 atgccgttcc tcgaggacgg cacgccggtc gacatcatct tgaacccgct gggcgtcccg
     9241 tcgcggatga acctcgggca ggtgctggag gcccacctgg gctacgccgc gctcgtcgga
     9301 ttcgacaaga gcggcgagga tcacaccgga cccgtccacg ttgccacgcc ggtcttcgac
     9361 ggcgccaagg aggacgagat cctcgcggcg atccgcagct cgcacgcggc ctccgacggg
     9421 aagaagctca tcaacgacat cggaaaggcg cggctctacg acggccggaa cggtcagccc
     9481 tacgccgagc cggtcacggt cggggtggcg tacatcctga agctgctgca tctggtggac
     9541 gacaagatcc acgctcggtc cacggggccg tactcgatga tcacgcagca gccgctcggc
     9601 gggaaggcgc agttcggcgg ccagcggttc ggggagatgg aggtctgggc cctggaggcc
     9661 tacggcgccg cgtacgccct gcaggagctg ctgacgatca agtcagacga cgtcctgggg
     9721 cgtgtgaagg tctacgaggc catcgtgaag ggggagaaca tccccgagcc gggcatcccg
     9781 gagtccttca aggtgctgat caaggagatg cagtcgctgt cgctcaacgt cgaggtgcgc
     9841 tcctcgtccg gtgaggagat cgagctgcgc gagaccgacg aggacgtgtt ccgggcggcc
     9901 gaggagctcg ggatcgatct cagccgacgg gagccggcgg gggcggactt cgattgaggc
     9961 gcgggaatac ggaagcgttc cacatgatgg gggacgggct ggggggtctg gggactcaag
    10021 cagcgagcac gtcagcgcga gcggtagccc ccaggaaccg aaggaggcgg aggtctaagt
    10081 gctggatgtg aactacttcg acgagcttcg gatcgggctg gcagcggccg accagatccg
    10141 cgcctggtcc aacggagagg tcaagaagcc ggagacgatc aactaccgca cgctgaagcc
    10201 ggagaaggac gggctcttct gcgaacggat cttcggtccc acgcgggact gggagtgcca
    10261 ctgtggcaag tacaagcgcg tgcgattcaa gggcatcatc tgcgagcgct gcggcgtcga
    10321 gatcacccgg tccaaggtcc gccgcgaccg catgggtcac atcgagctcg cggctcccgt
    10381 cacgcacatc tggtacttca agggcgtccc ctcgcgtctc gggtatctgc tcgacatcgc
    10441 ccccaaggac ctcgagcgga tcatctactt cgccgcctac gtggtgaccc gggtcgacga
    10501 cgaacggcgt cacaaggacc tccccgagat cgaagaggag atcgagggcg agaagcgcga
    10561 gatcgaagcc gagcgggacg accaggtcaa gcgccgactc tcggagctcg aggaccgtct
    10621 gaaggagctc gaggccgagg gcgccaaggg cgctcagctc tccgccgcca agcgggaggc
    10681 gcagcgagac gtcgagcgga tccacgaggc cgccggtcgc gacacccagc acctcgacga
    10741 cgtggtgtcc gcgttcaaga gcctcaagat caagcagctc gtcgccgacg acgccgtcta
    10801 ccgctcgctc cgcgagcgct tcgcggacta cttcgacgga ggcatgggcg ccgaggccat
    10861 caagcggctg ctccaggact acgacgtcgt cgccgaggga gaggccctcc gcgagacgat
    10921 cgagaacggc aagggcacga agcgcctcaa ggccatcaag cgcctgaagg tggcctccaa
    10981 cttcgtcgag gggcgttcga gcccgatggg catggtgctc gatgcgatcc cggtgatccc
    11041 gccggacctc cgtccgatgg tccaactgga cggcggccgg ttcgcgacct cggacctcaa
    11101 cgacctgtac cggcgtgtca tcaaccggaa caaccggctg aagcggttgc tcgacctgca
    11161 agcacccgag atcatcgtga acaacgagaa gcgcatgctc caggaggccg tcgacgctct
    11221 gttcgacaac ggccgtcggg gtcgtcccgt cacgggaccg gggaaccggc cgctgaagtc
    11281 gctctccgac atgctgaagg gcaagcaggg ccgattccgc cagaacctgc tcggcaagcg
    11341 cgtcgactac tcgggccggt cggtcatcgt ggtcgggccg gagctccgcc tgcaccagtg
    11401 cgggctgccg aagcagatgg cgctcgagct cttcaagccg ttcgtgatga agcggctcgt
    11461 cgaccataac ctcgcccaga acatcaagag cgccaagcgc atggtggagc gtgcccgtcc
    11521 gcaggtgtgg gacgtgctcg aggaggtcat ccgggagcac cccgtgatgc tgaaccgggc
    11581 gcccacgctg caccgtctgg ggatccaggc cttcgagccc gtgctggtcg agggcaaggc
    11641 catcaagatc caccccctcg tctgcgcggc cttcaacgcc gacttcgacg gggaccagat
    11701 ggccgtgcat gttccgctgt ccgccgaggc gcaggccgaa gcccggatcc tgatgctctc
    11761 gacgaacaac atcctgtcgc cggcgcacgg ccgcccgatc gcgacgccgt cgcaggacat
    11821 ggtgctcggg ctctactacc tgaccctcac gcagaacgag gacatgaagg acgaggagat
    11881 cccgacgttc ggctcgaccg ccgaggcgtt gctggccctc gatcgcggtc atccgctgca
    11941 cgatccggtg cgcgtgcgct tgacgggtca ggcgctcccc gaggagctcc tcccggaggg
    12001 ctacgacccc aaggccgatg gatggcaggg ccgcacgccg gtcatccgga cgaccatcgg
    12061 ccggatcgtc ttcaacacgg cgttcccgga cgacttcgag ttccgaaacg agcccgtcct
    12121 gaagccggac gtggcgcgga tcgtcgacga ggtggtccac cgctacgacc gggcgcaggt
    12181 cgagatcgtc ctcgacaacc tgaagaatct cgggttcgac tacgccacca aggcgggggt
    12241 cacgatcggc gtcgaagacg tcacgacgcc accggagaag cagaagatcc tcgacgagca
    12301 cgagaagcgt gcccagaagg tcgagcagca gtaccggaag ggcatcatca cggacgacga
    12361 gcgtcgccag gagctcatcg agatctggac gcaggccacc gacgaggtca aggacgcgat
    12421 ggaggctcag ttcacgaaga cgaacccgat cttcatgatg gccaactctg gcgcgcgcgg
    12481 gaacatcatg cagatccggc agctggccgg gatgcgcggt ctcgtcgcga acccgaaggg
    12541 cgagatcatc ccgcgcccga tcaagtccaa cttccgcgag ggtctgtcgg tcctcgagta
    12601 cttcatctcc acccacggcg ctcggaaggg cctggccgac acggccctgc ggaccgccga
    12661 ctcgggttac ctcacccggc gactcgtcga cgtcgctcag gaggtcatcg tccgcgagga
    12721 ggattgcggc accgatcgat cgatcggtgc ggtggtgaac gtcacccgtc ccgacgggac
    12781 cctcgtgaag ggcgcccaca cggacaccgc gttgttcgga cgcgtcctcg cggaggacgt
    12841 gaaggtcggc ggcaagaaga tcgccgcccg tggggccgag ctctccgacg agctcgccga
    12901 ggccgtcatc gactccggcg tggagacggt ccaggtccgc tcggtcacca cctgtgaggc
    12961 ggagatcggc gtgtgccagg cgtgctacgg ccgctcgctc gcgagcgggc gcctcgtcga
    13021 catcggggag gcggtcggca tcatcgccgc ccagtcgatc ggcgagcccg gcacccagct
    13081 cacgatgcga accttccaca ccggcgggat cgccggcgag gacatcacgc acggcctgcc
    13141 ccgggtggtc gagctcttcg aggcgcgcaa tcccaagggg atggcgcaga tcaccgagct
    13201 ctcgggtgag gtggcgatcg aagacgacga cgagaagcac gtccggcgca tccgggtcac
    13261 ggacgagacc ggcgacatgg tcgagtacca ggtctcgttc cgcgcccggc tcacggtgtc
    13321 gaacggcgac atggtggagg ttggtcagca gctcacggag ggctcggtga acccgcacga
    13381 gaagctccgc gtggagggag tgctggccct gcagatgcac ctcgtggacg aggtgcagca
    13441 ggtctaccgc tcccagggcg tgacgatcca cgacaagcac atcgagctga tcgcgcggca
    13501 gatgctccgg aaggtgcaca tcatcgagcc gggggactcc gatctgctgc ctggcgatct
    13561 cgtgaaccgg cgggtgttcg aggagacgaa ccaggaagtg gtcgagtccg gcggggtacc
    13621 cgcatcggcc cggcccgtcc tgctcggtat cacgaaggcc tcgctcgcga cggattcgtg
    13681 gctctcggcc gcctcgttcc aggagacgac ccgggtgctc acggacgcgg cgatccagag
    13741 caaggcggac ccgttgctgg gcctcaagga gaacgtgatc atcgggaagc tgatcccggc
    13801 gggcacgggg atgagccgct accgcaacgt ggccgtgaag atcaagcccg atgcgatccc
    13861 cgagtactgg ctcacgcgtc agcgggagct ggcggagctg gcggagctgg cagaaggtgg
    13921 cgagccggct ctggtggggc tcacccgcca gcaggccgag agcatgctgg gcggggtcgc
    13981 tcgatccgac gaggagtagg ccggggaccg ggctctcggt ttccgagagc ccggccggac
    14041 gtgtcgaggg aagagctcgg caggtaccct agggatggtt ccgcgtcggc cggccctcac
    14101 gccggtggca tccggtgcgg ttgacacgtc gtgctcacga ccagtagctt tggccgtcgc
    14161 gcccgggtcg gcgagcaggt cgggcgtccg gctccggtcg gagcgacaac cccgcgggtg
    14221 gtggcgttcg cgcgctgcgc cccagggttc ggcccgaggc ttccaagccc cggtggatga
    14281 aaaaggaaga gtgcagacga gagagccatg cctaccatcc aacaactcgt acggaagggc
    14341 cgagcccgcc cgaagaagcg gacgaagagc cctgcgctga agggctcgtc ccagcggcgc
    14401 ggcgtctgca cgcgtgtgta cacgatgacc cccaagaagc cgaactccgc cctgcggaag
    14461 gtcgcgaggg tccggctcac ctccgggatc gaggtcacgg tctacatccc cggagtgggg
    14521 cacaacctcc aggagcactc gatcgtgctc gtccgagggg gtcgcgtacg cgacctgccc
    14581 ggtgtgcgat acaagatcat ccgcggcacc ctcgataccg caggggtccg cgaccgacgc
    14641 aaggcccggt cgcgctacgg cgccaagaag gtcggcgagt aacgtgccgc gcaagggacc
    14701 tgccgtacgc cggggcatca cgcccgaccc cgtctaccag aacccgctcg tgacccagct
    14761 ggtcaacaag gtgctgttgc acggcaagaa gaccgtcgcc gagcacatcg tctacgaggc
    14821 gctcgagcag atcagtcaga agaccgcgaa cgatcccgtg atcaccctga agcgtgcggt
    14881 ggacaacgtt cgcccgatgc tggaggtcaa gtcgcgacgg gtcggcggag cctcgtatca
    14941 ggtccccgtg gaggtgaagc ccacccgtgg cacgacgctc gcgctgcggt ggctcgtgaa
    15001 cttctcccgc gcgcggcgcg agaactcgat ggccgaacgt ctcgtggcgg agatcatgga
    15061 tgcctccaac gggcaggggg cgtccgtcaa gcggcgcgag gacatgcaca agatggccga
    15121 ggccaaccga gcgttcgcgc attaccgatg gtgatcgacg agcaccaccc gaccggccct
    15181 atggggccgg tttcccgtgt ggaggggacc tcctgatggc ggtccccgca gagaggatgc
    15241 aggtgtcgca gaccgagcag aaggtgaagg acggcaaggg gctctcgatc cgcgagtacc
    15301 ccctcgctcg cacgcgcaac atcggcatca tggcgcacat cgacgcaggg aagaccacca
    15361 cgaccgagcg gatcctctac tacaccggga aggcctacaa gatcggcgag gtgcacgaag
    15421 gcaccgcgca gatggactgg atggtccagg agcgcgagcg cgggatcacg atcacgtcgg
    15481 cggccaccac gtgccagtgg aagggccact ggatcaacat catcgacacg cccggccacg
    15541 tcgatttcac ggtcgaggtc gagcggtccc tccgcgtcct cgacggcgcc gtcgccgtgt
    15601 tcgacgccgt tgcgggcgtg gagccccaga ccgagaccgt gtggcgccag gcggaccgct
    15661 acaaggtgcc ccggatctgc ttcatcaaca agatggatcg gaccggtgcc aacttcgagc
    15721 gcaccgtcga catgatcgtg gatcgcctcc aggcgacccc gctcatcctg cagctcccgt
    15781 ggggcaccga gtccgacttc cacggcgtga tcgacctcgt ccagatgaac gcccactact
    15841 gggagggcga catgggcgag gagtggcacg acaccgagat cccggaggag taccgcgccg
    15901 ccgcggaggc tgcccaccac gtcctcttcg agcggctcgc cgaccacgac gagtccctga
    15961 tggagaagtt cgtccacgag gaggagccca cgatcgagga gatgcatcgc gcgatccgcc
    16021 gagcgacgct cgcgagcctc ggcgttccgg tgatgtgcgg ctccgccttc aagaacaagg
    16081 gcgtccagcc tctgctcgac gcgatcgtcg cctacctccc gtcgcccgcc gaggtgctcc
    16141 cggtggaagg ccatcttccc aacgacgagt ccacgcacgt ccagcgggcg cccgaggaca
    16201 ccgagccctt cgcggccctg gccttcaaga tcatgtcaga tgccttcgtc ggccggctga
    16261 cgtacctgcg cgtgtactcc ggcgtgctcc gcgccggctc gcacgtcgcc aacgccacga
    16321 aggaccgcaa ggagcggatc ggtcggattt tgcagatgca cgccaaccac cgtgaggaca
    16381 tggcggccgc ctacaccggc gacatcgtcg ccgtcgtcgg gttgaagcac gccaccaccg
    16441 gagacacgat ctgcgatccc gagcaccagg tcgtcctgga gtccatcagc ttccccacgc
    16501 ccgtcatctc ggtggccgtc gagccgaaga ccaaggcgga ccaggacaag ctcggcaccg
    16561 gtctcgccaa gctcgcggac gaggacccga cgttcgtcgt gaagttcgac gacgagacgg
    16621 gccagacggt gatctcgggg atgggggagc tccacctcga catcatcgtg gaccgcctga
    16681 agcgagagtt ctccgtcgac gcgaacgtcg gcaagccgca ggtcgcctat cgcgagacga
    16741 tccgccgcca ggtccacaag gtcgagaccc ggttcgtccg acagacgggc ggccgcgggc
    16801 agttcgccca cgtcgtgatc gacctggagc cgaccggtgc cggtgggggc tacgagttcc
    16861 tgaacaagat cgtcggcggc aagatcccgc gggagtacat ccccgccgtc gaccagggga
    16921 tccaggaggc cctcgacaac ggcgtgctgg ccggttaccc gatggtcgac gtccgcgcga
    16981 cgctgatgga cggcacctac cacgaggtcg actcgagcga gatggcgttc aagatcgcgg
    17041 gctcgatggc gctgaaggag gcggctcgca aggccgaccc cgcgctgctc gagccggtca
    17101 tggagttcga ggtgaccacc ccggaagagt tcatgggcga ggtcatgggc gacgtgacgg
    17161 cgcggcgggg ccggatcgag cgcatcgacg atgcgggagg ccagaaggtg atcagggtgc
    17221 tggtgccgct cgccgagctg ttcggctacg ccaccgacct gcgctcccgc acccaggggc
    17281 gcgccgcgca cagcccgatg cagctccacg cgtacgcaga agtgccagtg caggtgtcca
    17341 aggagatcat cgcccgggtc tcgggcgagt aggggcccag gaagagctgg aagagaagag
    17401 caggaagagg agagccaagt catgggcaag aagaagttcg agcgcacgaa gccgcacgtg
    17461 aacatcggca cgatgggtca catcgaccac ggcaagacca cgctcacggc cgccatcacc
    17521 aagcggcagg ccgagaaggg gatggccgag ttcacgccgt tcgacgagat cgacaaggcg
    17581 ccggaggagc gcgagcgcgg gatcacgatc gcgatcgccc acgtcgagta cgagacggac
    17641 aatcgccact acgcgcacgt cgacatgccg ggacacgccg actacgtgaa gaacatgatc
    17701 acgggtgccg cgcaggtgga cggcgcgatc ctcgtcgtct cggcggccga cgggcccatg
    17761 ccccagaccc gtgagcacgt gctgctcgca cgtcaggtcg gcgttccgtc gatcgtggtc
    17821 gcgctgaaca agtgcgacat ggtcgacgac tccgagctgc tcgacctcgt cgagctcgag
    17881 gtgcgcgagc tcctgtcctc ctacgagtat cccggcgacg acatcccggt cgtccagatc
    17941 tccgccctga aggcgctcga gggtgacgag acgtggatgg ccaaggtcga cgagctcctc
    18001 gaggcctgcg acacgtatat ccccgagccc gtccgggaca cggacaagcc gttcctgatg
    18061 ccgatcgaag acgtgttctc gatcaccggg cgtgggacgg tcgtgaccgg gcgcgtcgag
    18121 cagggcgtcc tgaagaagat gggcgaggtc gagatcgtcg gactccggga cacgcagaag
    18181 acgaccgcga cggacctgga gatgttccgg aagctcctcg atgagatcac ggccggcgac
    18241 aacgtcggcg tgctgctccg aggcatcgac aaggaccagg tggagcgcgg tcaggtcctg
    18301 gcgaagccgg gatcgatcac gccgcacacc gagttcatgt cgaacgtgta catcctgtcc
    18361 aaggaggagg gcggccggca cacaccgttc ttcgacggct acaagccgca gttctacttc
    18421 cgaaccaccg acgtcaccgg cgtggcacat ctgcccgaag gggtggagat ggtgatgccg
    18481 ggcgacaacg tggagatgcg ggtcgagctc gacaagccga tcgcgatgga ggagggcctc
    18541 cggttcgcga tccgcgaagg cggccgcacc gtcggggccg gccgtgtcac cacggtcctg
    18601 aagtagcggc cccaggaaac acagaaggcg acacagagga accacagagg gacgaggaag
    18661 gcgaggggac gacgtggcgg gaccgaagat ccggatcaag ctccgggcgt acgaccaccg
    18721 gatgctcgac gtcgccgccc gtgacatcgt cgagcacgtg acgcggaccg gcgccaaggt
    18781 cgtcgggccc gtccccttgc ccaccgagcg ttcgatctac acggtcatcc gctcgcccca
    18841 caagtacaag gactcgcgcg agcacttcca gatgctcacg cacaagcggt tgatcgacat
    18901 cgtggacgcg acgtccaaga cggtccagga gctccagcgc ctgaacctgt cggccggcgt
    18961 cgacatcgag atcaagttgt aggagcgatg gcggagacga aagggatctt gggcacgaag
    19021 ctggggatga cccagatctt cgacgacacc cgcgccgtgc cggtcacggt gatcaaggcg
    19081 ggcccgtcgg tcgtgtccca ggtcaagacg aaggagaccg acggctacga cgccgtccag
    19141 ctctccttcg gcaccgtccg gccgcgcgac ctcacgaggc cgatgaaggg acacttcgag
    19201 tcgcacgacg cgacgcccgg ccgccacgtc gtcgagctgc ggacggacga tgcgggcagc
    19261 tacgagccgg gccaagagat cagggtcgac gtgttcagcc cgggcgaccg ggtggacgtg
    19321 gtcggcgtct cgaagggtca cggcacgacc ggcgtgatga agcgccacgg gttccacggc
    19381 ctccgggcca gccacggcac gaagcgcaag caccgtgcgc ccggcgcgat cggtgcttgc
    19441 gcgacgccca gccgggtctt caaggacatg ccgatggccg ggcagcacgg cagcgtccgc
    19501 gtcacggtgt tgaacctcga ggtcgtccag gccgacgtcg agcgcggcct cctgctcgtc
    19561 aaaggagcgg ttcccgggcc gaacggtggc ctgctgatgg cgcgttccgc cgtcaaggcg
    19621 ccggcgaagg gaggcgcgtg atggcctcga tcgagaaacg cgacgccgcc gggaagaagg
    19681 ccggctccgt ggagctgccg gccgagatct tcgagtcgaa ggtgagcgtg cccctgatgc
    19741 accaggtggt cgtggctggc ctggccggga tccgcgcggg gacacactcg accaagacgc
    19801 gcgcccaggt ccgtgggggc ggcaggaagc cgtggcgcca gaagggcacg ggccgtgcgc
    19861 gccagggatc gatccggtct ccgcagtggg tcggcggcgg cgtggcccac gggcccactc
    19921 cccgggacca ctcgcagaag atcaacaaga agatgcgacg cggagcgctc cgctccgcgc
    19981 tgacggacgc gctgcagagc gggaagctcg ccgtggtcga cgacctcgat tggagcgagc
    20041 cgaagacgaa gcaggccgtg ggcgtgctcg cgtcgctcga gctcgccgga cggatcctcc
    20101 tcgtcctgcc ccagcccacg gagtccggca acgtcgagaa gtcgttccgc aacctgccca
    20161 acgtgaagat cgcctacagc ggagggctcg gcacctacga cgtcctcctg gcggaccggg
    20221 tgctgttcac ggatgccgcg atcgaccgct tgaccgagcc gccggcaggg tctccgaccg
    20281 gtgcctcgga agggtccgcc ggtcaggtcg aggaggtggc gggatgaaca agtcaccacg
    20341 cgacgtgatc gtgcgcccca tcgtctcgga gaagtcctac gcggggatcg agcacaacac
    20401 ctacaccttc ctggtggacc ctcgtgcgaa caagaccgag atcaaggagg cggtccagac
    20461 gatctgggac gtccaggtcc tgtccgtcaa cacgctgaag cgtcgcggca agatgaaacg
    20521 tcgcgggtac acgcgcggac gccgtccgga cgagaaacgc gcggtcgtca cgctcgcaca
    20581 gggcgacagc atcgagatct tcgagggtgg ggggtcctag ccgtggccgt tcggaaatac
    20641 aagccgacga cgccgggccg gcgtgggggc agcgtctcgt cgttcgatga gatcacgcgg
    20701 tccacgcccg agaagtcgct gctgaccaag ggccggaaga aggcgggacg caacgtccac
    20761 ggccggatca ccacccggca tcagggtggc ggcacgaagc ggcggtaccg cctcatcgac
    20821 ttcaagcgca acaaggacgg gatcccggcc aaggtcgcca cgatcgagta cgacccgaac
    20881 cggagctcgc ggatcgccct gctgcactac gccgacggcg agaagcggta catcctcgca
    20941 cccgacggcg tccgcgtggg cgaccagctg ctgtccgggg agggtgcgga gatccgcccc
    21001 gggaacgccc tgccgctgcg gaacatcccc gtcggcaccg tggtgcacgc ggtcgagctg
    21061 aagcccgggg ccggagcccg gatggcgcgt tcggcgggaa cgagcgtcca gttgatggcg
    21121 aaggagggga agctggccac gctgcggctt ccctcaggcg agatgcggac ggtcccgtcc
    21181 atctgcaagg gcaccgtcgg agtggtcggg aacgccgagc acgagctgat cgcgatcggc
    21241 aaggcggggc ggaaccggtg gaagggcaag cggccctcgg tgcgtggcgt cgccatgaat
    21301 ccggtggacc acccgctcgg cggcggggag gggaaggcct ccggtggacg ccatccgacg
    21361 tcgccgtggg gcaagaagga aggccgcacg cggaagaaga agaaggcgtc gaacgcgcag
    21421 atcatccggc gtcgcgggaa ggggcgtggc tgatggcgag gtccgtgaag aaggggccgt
    21481 tcgtggacga gcacctcatg gtgaagatcg cggggatgaa cgaacggagc gagaagaagg
    21541 tcgtcaagac ctggtctcgc cgttccacga tcgtcccgga catgatcgga cacacgatcg
    21601 cggtccacga cggccgcaag catgtccccg tgttcatctc ggagtcgatg gtcgggcaca
    21661 agctcggcga gttcgctcct acgcgcacct tccggacgca ctcgagggat gagcgaagga
    21721 cgggtccccg atagatggaa gcgaaagcca cggtcaagta cgtccgcacg tcaccgcgca
    21781 agatgcggcg aacggtggat ctcatccgcg gtcagcacgt gggtgaggcg aagcgcatcc
    21841 tccggctgtc gacgctgggg gcgtcacgcg acgtggagaa gctgctcgat tcagcgatcg
    21901 ccaacgccga gcagcggccg ggcgtgatcg cggagaacct gttggtatcc aaggcatggg
    21961 tggacgaggg gccgacgctc cgccggttcc gtccccgggc ctacggccgg gccacccggg
    22021 tccggaaacg cacctcgcac gtcacggtcg tggtggagac gatgggagac gaagagtagt
    22081 gggacagaag gttcacccat acgggttccg gctgggggtc atctacccct ggaaatcgaa
    22141 ctggtacgcg aaccggcgcg actacatcga ggcgctccac gaggacgtgt ggatccgcaa
    22201 acacatccgc tcacggctgt ctcgcgccgg gatctcctcg atcgatatcg aacgcaaggg
    22261 cgatcagatc tgggtctaca tccggacggc ccgccccggc atcgtcatcg gccgcaaggg
    22321 cgccgaggtg gaccgcctgc gcaaggacgt cgagcgctac acgaagaagc gggtggacgt
    22381 gaaggtcgag gacatgaacc aggcctccag cgaggtgcgg cccgacacgg acgccgccct
    22441 gctcgcgcag ggcgtcgccg agcagctcgc cggacgcgtg gcgttccggc gggcgatgcg
    22501 ccgggccgtc cagaccgcga tgcgcgctgg tgcgctcggc gtgcgcgtcg cgtgcgcggg
    22561 gcgcctcgga gggaccgaca tgggccggaa ggagtggtac cgcgagggcc gggtgccgtt
    22621 gcacacgctc cgggcgaagg tggacttcgg gacggcggct gcgaagacga cgttcggcgc
    22681 gatcggtgtg aaggtgtggg tctaccacgg cgacgaggtg ccccaccgcg agcaggagag
    22741 cgagcgggct ctggcgcggg cacacgcccg tgccgagcga ggcgagcggg gggcgaggcc
    22801 ggctgccacg cgcggcaagg ccccggccaa ggccgagccc gttgcggcga cacccgacgc
    22861 cgtcgcacca gtcgaggaac cgtcccccac gccgcccgag tccggtccgg aaccggcttc
    22921 cgtgaccccg gaagcgacgg acgatgccga gccggccgct gcaccggagg ccgcaccggt
    22981 ggccgaggcg cccgtcgaag cgcccgacac gcaggaggtt ggtgagggct gatgctcgct
    23041 ccgaagcgga tcaagcaccg caagcagcac cgtgggcgga tgcgcggcct cgccaagggc
    23101 cacaccgaca tccagtcggg cgactacgcg atcgtcgcga tggagccggg ctggatcacg
    23161 aaccgccaga tcgaggctgc ccgggtggcg atcaccagac acatccgccg cggcggcaag
    23221 gtctggatca cgatcttccc cgacaagccc gtcacgaaga agcccgcgga gacacggatg
    23281 ggctccggca aggggaatcc cgaaggctgg gtcgccgtgg tcaagccggg tcgcgtgatg
    23341 ttcgagctgg ccggagtgaa cgaggcgctc gcccgggaag cgatgggtcg cgcgcagcac
    23401 aagctgccca tccgtaccaa gttcatcacg agggtggggg agtagcgatg gccagcaaga
    23461 ccgccgagct gcgcgagctg acggatgcgc agctcctcga gcgggcggag tccgcgaagg
    23521 aagagctctt caacctccgc ttccagctcg ccaccggcca gctggacgac tcgtcgtcga
    23581 tcaagaaggt ccgccacgag atcgcgcgga tcgccacggt gatgcgccag cgggacatcg
    23641 aggctgcggc cacggatgcc gaggccggag cggcgggaac cgagtccgga acggagagcg
    23701 agcggtgagc gaggagcagc aggtgacgca ggagcgcggg cgccggaaga cgcgaacggg
    23761 ggtcgtggtc tcggacggca tggacaagac cgtagtggtt cggatcgacc gctcgttccg
    23821 ccacccgctg tacgacaaga ccgtgcggcg ctccaagaag ctcgccgccc acgacgagtc
    23881 caacgaggcg cgcgtgggcg accgcgtccg tgtcgtggag acgcgaccgc tgtccaagtc
    23941 gaagcactgg cggctggtgg agatcgtgga gaaggccaag tgatccagca ggagacccgt
    24001 tgccgtgtcg cggacaacac cggagccaag gagctcttgg tgatccgcgt gctcggcggg
    24061 tcggcccgcc gctacgcgag catcggcgac accgtcgtcg gcacgatcaa ggatgcgctg
    24121 cccggaggcg gggtcaagaa gggggaggtc gtcaaggccg tcgtcgttcg gacctccaag
    24181 gaacggcgtc gtcccgacgg ctcctacatc cggttcgacg ataacgccgt ggtgctcctc
    24241 acgccggact cgcgcaaccc ccgagggact cgcatcttcg ggccggtggc ccgggagctc
    24301 cgcgaccgcc gctacatgaa gatcgtgtca cttgcgccgg aggtgttgtg agggtggtgt
    24361 tctgaggcgt gccgggagtc gacatcaaga aggacgacac cgtccgggtg atctcgggca
    24421 aggatcgcgg ccgggaaggc cgcgtcgtac gcgtgctccc tcgggagggt cgagtgatgg
    24481 tggatggggt cgccctcgcc aagaagcacc agcgctcgtc gggccgccgc gcgcgcggca
    24541 gctcgcagca gctgcagcag ggcgggatca tcgacgccga gatgttcgtc aacatctcca
    24601 acgtccaggt ggtctgcacg gcctgcggcg aggcgacccg catcggtcat cgggtcgaag
    24661 gggatcggaa ggtccggttc tgccggaagt gcgagacgga gctgccatga gcgc
//
LOCUS       DASUYD010000116         7724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1604280, whole genome shotgun sequence.
ACCESSION   DASUYD010000116 DASUYD010000000
VERSION     DASUYD010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604280"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            778..1089
                     /locus_tag="VF195_06400"
     CDS             778..1089
                     /locus_tag="VF195_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580483.1"
                     /translation="MTGGLGGELNMGGYDAGGLGVSVSVVVSILALGLIPATSQWLIL
                     RPNPPTWTSFSIRFVIGLAIGGASGWLAATGLGLTLPSGAAWIADGATRAPFLAASVA
                     G"
     gene            complement(1271..1723)
                     /gene="rplI"
                     /locus_tag="VF195_06405"
     CDS             complement(1271..1723)
                     /gene="rplI"
                     /locus_tag="VF195_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018542657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6580484.1"
                     /translation="MKIILQKPVDKLGVPGDVVDVADGYARNYLMPRGLAVQATKGGV
                     KHVESLKRAHSTRVNAAKAEVEQVAQRLTASPITVAAHAGEEGRLFGSVTPSDVADAL
                     EAQTGVRVDRHDVHMDEPIRSVGAHEVRVHLFAEVDPVISVLVTASEE"
     gene            complement(1720..1992)
                     /gene="rpsR"
                     /locus_tag="VF195_06410"
     CDS             complement(1720..1992)
                     /gene="rpsR"
                     /locus_tag="VF195_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011562738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6580485.1"
                     /translation="MPPKTKKRPAKREKDDKGWQKKQKRKICIFCKDRIEYVDYKDTA
                     TLRRFVSERGKIRARRVTGNCSQHQRDVATAVKNAREMALLPYSTR"
     gene            complement(1976..2425)
                     /gene="ssb"
                     /locus_tag="VF195_06415"
     CDS             complement(1976..2425)
                     /gene="ssb"
                     /locus_tag="VF195_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6580486.1"
                     /translation="MASENQVTIVGNLTDDPELRYTPNGAAVVKFRVAVNRRYKDESG
                     NWKDGETSYFSVNAWRSLAENCAETLTRGTRVVLSGRLQMRSWETQEGDKRTVVEIEA
                     DEVGPSLRWATAKVERQGRSGGSSDWTPATVGAPAADEETPLDAPQN"
     gene            complement(2496..2822)
                     /gene="rpsF"
                     /locus_tag="VF195_06420"
     CDS             complement(2496..2822)
                     /gene="rpsF"
                     /locus_tag="VF195_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005882616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6580487.1"
                     /translation="MGAVQTRGGGELRQYEGMLILPAEADESVVSGALDRITRVIGEA
                     GGQVGNVDRWGRRRLAFEIERQAEGYYVVVDFTADPEVLTELERSLRLADEVLRFKIV
                     VRGEAA"
     gene            2975..3760
                     /gene="panB"
                     /locus_tag="VF195_06425"
     CDS             2975..3760
                     /gene="panB"
                     /locus_tag="VF195_06425"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6580488.1"
                     /translation="MTVTIRDLQAWKAEGTRFAMLTAYDFPTAKIFDEAGIPVLLVGD
                     SVGNNVLGYENTLPVTMEEMLHHTRAVARGVTNALVVGDMPFLSYQVSVEEGVRNAGR
                     FLKEGGAGAVKIEGAQIELVHKLVELGIPVMAHVGLTPQSVHAMGGYRVQGRGEEAAR
                     VTDQALALEKAGAFAIVLEAMPSELGIEITKSLHIPTIGIGAGPGTDAQVLVSPDLLG
                     LSERVPKFARAYVNLRGEIAKAATAFATDVAAGTFPDEDHSYT"
     gene            complement(4006..4599)
                     /locus_tag="VF195_06430"
     CDS             complement(4006..4599)
                     /locus_tag="VF195_06430"
                     /inference="COORDINATES: protein motif:HMM:NF017949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580489.1"
                     /translation="MTTSDPIAEIIGDYRAFVAMQRDRLVARGMDIAPYPLSHLAVRV
                     ADWDLYVHQRTLLERHARANSENVWNGRPISLIVLSEPLDVLEGTPVSLVELIPPVHQ
                     RVYRMGLEHIGVVVGEDVDDFSRVHRAALTGQQFQSPDVEPYYVLFEDFTHVKFYRRS
                     LYDGAILQGARFDGFSHVDDWVPQRVVTATGPDPLPR"
     gene            4971..6329
                     /locus_tag="VF195_06435"
     CDS             4971..6329
                     /locus_tag="VF195_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580490.1"
                     /translation="MEIPETRYASTSDGVHIAYQVVGDGPGDLVFVPGFVFNVEQTWE
                     WPQMARFARRLASFARLILFDRRGTGLSDHIVPQETQLTLEARMDDIRAVMDDARSER
                     AALFGFEEGFGLCAMFAATYPDRVSALVAFAPAGLGLRDAERPWALSQQGWDEYLEDV
                     RHGWGTLAFAEEEGKWVWPDVGEDAEWFRQYATWMRRSVGPGDAVAFLEIDSKLDVRD
                     VLPTVRTPTLVVQRIGDQAISIEYGRYVAQQIAGAVLVELPGSNHGYMAPDQDEVLDQ
                     IERFSKALRDEEAELDRVLATVLFTDIVGSTERASELGDRSWKDLLERHHRAIRGLLA
                     RYRGREVDTAGDGFFATFDGPARAVRCAQQIVDAVRPLDLQIRAGLHTGECETIDGKV
                     GGLGVVIGSRVGGLAGPSEVLVSSTVKDLTAGSGLVFEDRGEHELKGVPDRWRLYRVV
                     SE"
     gene            6668..7027
                     /locus_tag="VF195_06440"
     CDS             6668..7027
                     /locus_tag="VF195_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580491.1"
                     /translation="MDYQQAVRATTKRLREDQQSGWSRLRQFLDEQGIRPTEVALAVI
                     YPDDVSELVAVVVQSRSRMFEILLDYPRGLDLSLAMSEATVSSSDQDAERIFNENVEW
                     VEAALTVLADKSEGGST"
     gene            7426..>7724
                     /locus_tag="VF195_06445"
     CDS             7426..>7724
                     /locus_tag="VF195_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014058635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX6580492.1"
                     /translation="MRSVTYSMGVSLDGYIVGPDGGFDWTAPDDEVFRSWIDEIRQVG
                     VHLLGRRLYETMLYWETADQDPSLDDARLEWAALWKPLPKVVFSATLSAVQGNARL"
BASE COUNT         1204 a         2539 c         2653 g         1328 t
ORIGIN      
        1 ctccgctccc agcggaaaac cccgtgggaa acccgatccg ctggtcgtgc ctgaagattc
       61 cttgtcactg tccctcccac ctgggcttcc acagggggaa aaccctgtgg acaggtgtgt
      121 accgctcgtc accgacatcc ggatcacatc ggtgtgattc tttccccggg agcggtgggc
      181 gtccaggggc gggctgaggt cgggggtcgc gacggctgct cgtcttcgcc tacgcgttgg
      241 gttcgtgctc gcggacgtcc attggcttcg gctccgctcg acgacgcccg tcgcgacccc
      301 tccccgcccg ccacggcgtg aatcggtgag gctccagcgc cggtgacgtc gcttcgagac
      361 ggacaacgcg gacgcgcaac ggcaccaggc tctcgagccg gtaggacatc gcataggtcg
      421 accgggtgac atctgtgagc gtccgtgtgg tcacgaacga tcagcttcgg gaaaggccgt
      481 gccgcccgat cccgggtagt gcagtgggtc ttggcgagct ctgtcgggct cgcgatcgcc
      541 gggggctctt gccggttcgc tcgccccgaa cggcggcgag ttcggcgtca cggctgcccc
      601 ttcggcggcc gcggggtccg caacttcggg cttcccgcca cggcagcgtt ctccgtacgg
      661 gggcgccggc ctcggcgtgg cccgatgggt ggtgctccgc ggccgcctgc gcaacgctgc
      721 gtggtgggcg cccgccacga tcggaggatg ggcgctccta ggagggacga tggaaccgtg
      781 acgggcgggc tcggcggaga gctcaacatg ggtggctacg acgcgggcgg tctcggcgta
      841 tccgtgtcgg tggtcgtcag catcctggca ctcggactga tcccggccac gtctcaatgg
      901 ttgatcctgc gcccgaaccc accgacctgg acgtcgttct cgatccggtt cgtgatcggg
      961 ctggcgatcg gaggtgcgtc gggatggctg gcggccaccg gcctcggctt gacgctccct
     1021 tccggagcgg cgtggatcgc cgacggcgca acccgtgctc cgttcctcgc cgcatccgtc
     1081 gcagggtagg cctcgcgcaa ccgagatctg cgaccgcgtg gcggagaggg gttcggtcag
     1141 ggcgtcgtcg agcgaagccg aagcccctcg aacgcccgcg aggacggacg atatgcgtag
     1201 gcgaagacga gcagcccgga ccgaaccccc gacctcagcg caaccccgcc gaggtcgggt
     1261 gcacggaccc ctactcctcc gaagccgtca ccaagaccga gatcaccgga tcgacctcgg
     1321 cgaacaggtg cacgcgaacc tcgtgcgcgc ccaccgagcg gatcggctcg tccatgtgga
     1381 cgtcgtgtcg gtcgacgcga acgccggtct gcgcctcgag ggcatcggcg acatcgctcg
     1441 gcgtgacgct gccgaacagc cggccttcct cgccggcgtg cgcggcaacg gtgatcggcg
     1501 atgcggtgag acgctgagcc acctgctcga cctcggcctt cgcggcgttc acgcgcgtcg
     1561 agtgagcgcg cttcaggctc tcgacgtgct tgacgccgcc cttggtcgcc tgcaccgcga
     1621 ggccacgagg catcaggtag ttgcgcgcat agccgtcggc gacgtccacg acgtccccag
     1681 ggacgccgag cttgtcgacg ggcttctgca ggatgatctt catcgggtcg agtacggcag
     1741 cagggccatc tcgcgggcgt tcttgaccgc ggtcgcgacg tcgcgctggt gctgcgagca
     1801 gttgcccgtg acccggcgcg cacggatctt gccgcgctcg gacacgaaac gccgcagcgt
     1861 ggcggtgtcc ttgtagtcga cgtactcgat ccggtccttg cagaagatgc agatctttcg
     1921 cttctgcttc ttctgccaac ccttgtcgtc cttttcccgc ttcgcgggtc gcttcttagt
     1981 tttggggggc atccagtggt gtctcctcgt ctgccgcggg agcgccgacc gtggcgggcg
     2041 tccagtcgct cgaaccgccc gaacgaccct ggcgctcgac cttcgcggtc gcccaacgca
     2101 ggctcggacc gacctcgtcg gcctcgatct cgaccaccgt gcgcttgtcg ccctcctggg
     2161 tctcccagga gcgcatctgc agccggcccg acagcaccac gcgtgtgccc cgcgtgaggg
     2221 tctcggcgca gttctcggcg aggctccgcc aggcattgac cgagaagtac gacgtctcgc
     2281 cgtccttcca gttgcccgac tcgtccttgt agcggcggtt gaccgccacg cggaacttca
     2341 cgaccgcagc cccgttgggc gtgtagcgca gctcggggtc gtccgtgagg ttgcccacga
     2401 tggtgacctg gttctcgctc gccatctcca tcaccttccg ttccgtgacc gctccccgtc
     2461 ggatggcgta ccggggaggg ggaagtcgct tctcgttacg cggcctcgcc gcgcacgacg
     2521 atcttgaacc gcaggacctc gtccgccagc cgcagcgaac gctcgagctc ggtcaggacc
     2581 tcgggatcgg ccgtgaagtc caccacgacg tagtagccct cggcctgccg ctcgatctcg
     2641 aacgcgagac gccggcgacc ccagcgatcg acgttgccga cctggccacc ggcctcgccg
     2701 atgacgcggg tgatgcggtc cagtgccccg gacacgaccg actcgtcggc ctccgcgggc
     2761 aggatgagca tgccttcgta ctgcctcagt tctccacctc ctctggtctg gacggccccc
     2821 acgagaggga gcaggatgtg tggcccccag agtctaccgg ctcactcggt cggccgaccc
     2881 tagcacccgc cgatgacacc cacgcagggt catgggcggt cgccgggtac actccggaga
     2941 ctgctgccgt gcactcacga aggaggcgcg agcgatgacg gtcaccatcc gcgacctgca
     3001 agcgtggaag gccgagggca cgcggttcgc gatgctcacg gcctacgact tccccaccgc
     3061 gaagatcttc gacgaggccg ggatccccgt cctgctcgtt ggagactcgg tcggcaacaa
     3121 cgtcctcggc tacgagaaca ccctccccgt cacgatggag gagatgctcc accacacgcg
     3181 cgcggtcgcg cgcggggtga cgaacgctct cgtcgtcggc gatatgccgt tcctctccta
     3241 ccaggtgagc gtggaggagg gtgtccgcaa cgccggccgg ttcctgaagg agggaggcgc
     3301 cggcgccgtc aagatcgaag gggcgcagat cgagctcgtg cacaagctcg tcgagctcgg
     3361 gatccccgtc atggcgcacg tcggcctcac ccctcagtcg gtgcacgcga tgggcggcta
     3421 cagggtccag ggccgcggcg aggaggcggc tcgggtcacc gaccaggcgc tcgcgctcga
     3481 gaaggcgggc gcgttcgcga tcgtcctcga ggccatgccg tccgagctcg gcatcgagat
     3541 cacgaagagc ctgcacatcc ccacgatcgg gatcggtgcg ggcccgggga cggacgccca
     3601 ggtgctggtc tcccccgacc tgctcggcct gtccgagcgt gtcccgaagt tcgcccgcgc
     3661 ctacgtcaac ctccgtggcg agatcgcgaa ggcggccacc gcgttcgcca ccgacgtcgc
     3721 cgccgggacg ttcccggatg aggatcactc ctacacatag agcgcgcaca gcagcgatga
     3781 agcaggcctc acgccctccg gcgtaggcgg cccgtgtagc gcatgtcggt agtcattcca
     3841 acatccgcgt cggatcggta gtggacgacg accgcggcgc gccggcctct ccaaggcgct
     3901 gggcgatgcc ggcgctgacg ccaccggacg tcctggtcac cagcgccggc cgagactcag
     3961 gagctccgca ttcaggagct ccgcaacggg cggcgccaga tccggttacc gcgggagcgg
     4021 gtccgggccg gtggcggtga ccacccgctg tggcacccag tcgtcgacgt ggctgaagcc
     4081 gtcgaagcgc gctccctgga ggatggcgcc gtcatacaag gagcggcggt agaacttcac
     4141 gtgggtgaag tcctcgaaca ggacgtagta cggctcgacg tcaggactct ggaactgctg
     4201 accagtcagc gcggctcgat gtactcgtga gaagtcgtcg acgtcctcac cgacgacgac
     4261 gccgatgtgc tcgaggccca tccggtagac gcgctggtga acgggcggga tgagctcgac
     4321 gagcgagacc ggagtcccct cgagaacatc gagcggctcg gagagcacga tcaaggagat
     4381 cggacgcccg ttccagacgt tctcgctgtt cgccctcgca tggcgctcga gaagcgtgcg
     4441 ctggtggacg taaaggtccc aatcggcgac gcgaacggcg aggtggctga gcgggtaggg
     4501 cgcgatgtcc atgccgcggg caacgagacg gtcgcgctgc atcgccacga aggctcgata
     4561 gtccccgatg atctcggcga tcggatcaga tgtcgtcacg tggcatcctc ctccgcgatt
     4621 gtacgaacgg gctcgtcctg gtccaatcga cattcccgca cactgggtga ccacggcgtg
     4681 ctgtttccct agaaaggggg ggttccgcat tggtttcgat catgtccaca cgccccggca
     4741 cccgccgcct gcgtccaacc cgatctacat ggagcgttgt ccgcggctcg aacgctttgg
     4801 gcgggtctgt ttgaggggga ggaagccgtg ttgcacgtga tgggctgggt gggcgcccgc
     4861 gccggcgtgg ccgttgcgtg gacgggccgg gcgcggaggt tcccggggtg atgtccgccg
     4921 gcatgctctc ccattgcagc ctgaggccgt aggggccgag gataagcctg gtggaaatcc
     4981 ctgagacccg gtatgcctcg acttcggacg gtgtccacat cgcctaccag gtcgtgggtg
     5041 atggcccggg cgatctggtg ttcgtgcccg gattcgtctt caacgtcgag cagacgtggg
     5101 aatggccgca gatggcgaga ttcgcgaggc gcctcgccag cttcgctcgc ctgattctct
     5161 tcgaccgccg gggaacgggt ctctcagacc acatcgttcc ccaggagacg cagctcaccc
     5221 ttgaggccag gatggatgac atccgggcgg tgatggacga cgccagatcg gagcgggctg
     5281 ccctattcgg gttcgaggag ggcttcgggc tctgtgcgat gttcgcggcg acctaccccg
     5341 accgcgtcag cgcgcttgtg gcgttcgccc cggccgggct aggtctgagg gacgccgagc
     5401 gaccgtgggc cttgagccag caggggtggg acgaatacct cgaggatgtc cggcacgggt
     5461 gggggacgct cgctttcgcc gaagaagagg ggaagtgggt gtggccggac gtcggcgaag
     5521 acgccgagtg gttccggcag tacgcgacgt ggatgcgccg ctcggttggc cctggcgacg
     5581 ctgtcgcgtt cctggaaatc gattcgaagc tcgacgtacg ggatgtcctg ccaacggtga
     5641 ggactccaac gcttgtcgtc cagcggatcg gtgatcaggc aatctcgatc gaatacgggc
     5701 ggtacgtcgc gcagcagatc gcaggtgcag tcctggtcga gctccccggg tcgaaccacg
     5761 gatacatggc gccggaccaa gacgaggtgc tagatcagat cgagcggttc agcaaggccc
     5821 tccgcgacga agaggccgag ctggaccgcg ttctcgccac ggtcttgttt accgacatcg
     5881 tcggctcgac agaacgggct tccgagctgg gagaccggtc gtggaaagac ctcttggagc
     5941 gccatcaccg agcgatccgt ggcctgctcg cccgctaccg gggacgcgag gttgacacgg
     6001 ccggggacgg attcttcgcg accttcgacg ggccggctcg agcggtccga tgcgcgcagc
     6061 agatcgtcga tgccgtccgt ccactcgatc ttcagatccg cgctggtctt cataccggtg
     6121 agtgcgaaac gatcgacggg aaagtaggcg gactcggcgt tgtcatcggt tcgagagtcg
     6181 gtgggcttgc tggtccgagt gaggttctcg tctcctcgac cgtgaaggac ctcaccgctg
     6241 gcagcggtct tgtgttcgag gaccgcggcg agcacgagct caagggtgtc cccgaccggt
     6301 ggcgcctcta ccgagttgtt agcgagtgag atagcgcagc tgggtggagc gccgcgcccg
     6361 gcgtggccat ttcgtggaca gccgtctggt cgcgctcacg gccggaacag gtccctcgcc
     6421 ccgggttgtg ggtgtggggg ccgagggcgg aaccgacatc caaggacgca agcgaatcac
     6481 cgagagcgcc ttcgacgggg ggcggcgttc ggggccgccc tatctcctaa ccccttgcgc
     6541 aggggttgga tatgcagccc gaacggactc cggtcgcgac cgtagaggtc cggcttggac
     6601 gaacgaccgg ctcgagcggc gtttcgccag cgtacgacgt atgctgccga gtgaatggcc
     6661 tatccctatg gattaccagc aagcagtccg agcgacgacc aagcgactac gcgaggatca
     6721 gcagtctgga tggagtcggc tccgccagtt tctcgatgaa cagggaatta ggcccactga
     6781 agtagcactc gctgtgatct acccggatga cgtgtccgaa cttgttgccg tcgtcgtcca
     6841 atcaaggtcg cgaatgttcg agatactgct cgactatccc cgtggtctgg atctaagcct
     6901 ggcaatgtct gaagccactg tgtcctcatc ggatcaagac gcggagcgaa tcttcaacga
     6961 gaacgtggag tgggtcgagg cagctctcac ggtcctggcg gataagagtg agggcggaag
     7021 tacatgagca ggagtccgcg gccacgctgc cccgtctcgc actcgggcgc ggctgccgtt
     7081 acggatgggg gcgttccggg gcccttgtct cccttccccc caaacgttgg accatcgttc
     7141 ggctcccaaa cagaccgtcg tccacgaaac ggccacgcgg acgcgggcgc cccacccagc
     7201 ctgtgttgca cgtgatgggg ctccctcgat gggctgtgtg tccgcgcgcg ctcgttgcgc
     7261 cacgcggcgc cccccgctgg tggccgggtg gccggccggt cgcggatcgt tcggaaggtg
     7321 cgcttgtcca gcctcgtcgc tagccagggg atcccgcgag ccgcgatgag tcctgcggcg
     7381 tccggccgtc gtactgtcgt gcccgtggcc gaaggaggac ttcggatgcg cagcgtgacc
     7441 tactcgatgg gcgtctcgct tgacggctac atcgtcgggc cggatggcgg cttcgactgg
     7501 acggcgcccg acgacgaggt ctttcgctcc tggatcgacg agatccgaca ggtcggcgtc
     7561 cacctgttgg gacgacggct gtacgagacg atgctgtact gggagaccgc cgaccaggat
     7621 ccatcgctgg acgacgcaag gctcgagtgg gccgcgctct ggaagccgct ccccaaggtg
     7681 gtgttctccg ccacgctgtc ggcggtgcag ggcaatgccc gcct
//
LOCUS       DASUYD010000117         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1606982, whole genome shotgun sequence.
ACCESSION   DASUYD010000117 DASUYD010000000
VERSION     DASUYD010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1606982"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VF195_06450"
     CDS             complement(<1..374)
                     /locus_tag="VF195_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6580493.1"
                     /translation="MPDLPVWEERYRAPTRTLPVWSPAMPDRFVLRSDESGSFQAYAW
                     EHGAEPRRLTDEPVGITLATVSGDGSSLVWFSDPTGDESGRWLAVPFEGGEPRELLPG
                     APVGWPEGLSLGRELVVAVLADR"
     gene            complement(397..843)
                     /locus_tag="VF195_06455"
     CDS             complement(397..843)
                     /locus_tag="VF195_06455"
                     /inference="COORDINATES: protein motif:HMM:NF022114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HEX6580494.1"
                     /translation="MTIVVGVVVLGLAATGCTGADRATEALSPSPPTSVGSPAPVTSS
                     SPAGEPSIVIETPFVNGEVANRVSITGTADVLDASLVVRVLDEHGFELAATVVEASCG
                     DGCLGTFAAELFFFVERRQRGTVQVSGVSGDGGPAVASVPVFLVPA"
     gene            972..>2020
                     /locus_tag="VF195_06460"
     CDS             972..>2020
                     /locus_tag="VF195_06460"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX6580495.1"
                     /translation="MRRRTAAIIGTFVVVQVVLAATRASAGSPIRAREVVGDLDQPVA
                     FTFAPGKKVWYVEKGTGQVRVHDLDTDSDRLFVTVPGVNGEGERGMLGIALHPRYPNK
                     PLVYVYATRSAQGQLRNQILRYRDDHGSGVGRKVLFSSIASGTPYHNGGRIAFGPDGM
                     LYAIVGDAHNSGNAQDTTNEDRGKIIRIEPNGDVPPDNPFGDRVWVFGIRNSFGFAFD
                     PLTDDLWETENGPSCNDEINLIERGENYGWGPNETCDGSSPGNTNQDGPSPVLPELFY
                     ENAIGITGIAFCEGCRLGQASDGAAFGGAVNNGEVTRIVFNGQRDAIAGHSVVYNHGG
                     STLSFEIGPGGRIFF"
BASE COUNT          356 a          696 c          708 g          260 t
ORIGIN      
        1 cggtcggcga gcacggcaac gacgagctca cggccgaggg agagaccctc gggccaaccg
       61 accggggcgc ccgggagcag ctcgcggggc tccccgccct cgaacggcac ggcgagccag
      121 cggcccgact cgtcgccggt cgggtcggag aaccaaacga ggctcgaacc gtctcccgag
      181 accgtcgcca gggtgatccc gacgggctcg tcggtcagcc gccgaggctc ggccccgtgc
      241 tcccacgcgt acgcctggaa cgaaccgctc tcgtccgagc gcaggacgaa gcggtcgggc
      301 atcgccgggc tccagacagg gagcgtccgc gtcggcgcgc ggtaccgctc ctcccacacg
      361 ggcaggtcgg gcatggtcgg caaccctagc aggggctcag gccgggacga ggaatacggg
      421 aacgctcgcg acggcaggac cgccgtctcc ggagacgccg ctgacctgga ccgtcccccg
      481 ctgacgacgc tcgacgaaga agaacagctc cgcggcgaag gtcccgaggc atccgtcccc
      541 gcagctggcc tcgaccaccg tcgccgcgag ctcgaacccg tgttcgtcga gcacgcggac
      601 cacgagggat gcatcgagca catcggccgt gccggtgatc gagacccgat tcgcgacctc
      661 cccgttcacg aacggggtct cgatgacgat cgagggttcg ccggcaggcg acgaagatgt
      721 cacgggtgcg ggcgatccca ccgaggtcgg aggcgacggc gagagcgctt ccgtcgcgcg
      781 atcggccccc gtgcagccgg tcgccgcgag gccgagcacg acgacgccca ccacgatcgt
      841 catccgatgc cccgacatcg ccgcggcgac gtatcgtggc gacgcccaga acccgcgccc
      901 gcgccgtgat ggaaaggacc gacgcccgat gatcgatgtg gtcatgcagg acgactgtag
      961 agggagctgt catgcgtaga cggaccgccg ccatcatcgg gaccttcgtc gtggtgcagg
     1021 tcgtgctggc agccacacgg gcgtcggcgg ggtcgccgat ccgcgcgcgc gaagtggtgg
     1081 gtgacctcga ccagccggtc gcgttcacgt tcgcaccggg caagaaggtc tggtacgtcg
     1141 agaaggggac cggccaggtc cgcgtgcacg atctcgacac ggactcggac cggctgttcg
     1201 tcacggtccc cggcgtcaac ggcgagggcg agcgcgggat gctcggcatc gcgctccacc
     1261 ctcggtaccc gaacaagccg ttggtgtacg tgtacgcgac gcgctcggcc caagggcagc
     1321 tcagaaacca gatcctgcgc taccgagacg atcacggctc gggggtgggt cgcaaggtgc
     1381 tcttctcgag catcgcgagc gggaccccgt accacaacgg cgggcggatc gcgttcgggc
     1441 ccgacggcat gctgtacgcg atcgtcggcg acgcgcacaa ctcggggaac gcgcaggaca
     1501 cgaccaacga ggaccgcggg aagatcatcc gcatcgagcc caacggcgac gtcccccccg
     1561 acaacccgtt cggcgaccgc gtgtgggtgt tcgggatccg gaactcgttc ggcttcgcct
     1621 tcgacccgct gaccgacgat ctgtgggaga ccgagaacgg cccgtcttgc aacgatgaga
     1681 tcaacctgat cgagcggggc gagaactacg gctggggacc caacgagacc tgcgacggct
     1741 cctcccccgg caacacgaac caggacggtc cgagccccgt cctgcccgag ctcttctacg
     1801 agaacgcgat cgggatcacc ggcatcgcgt tctgcgaagg atgccgcctc gggcaggcga
     1861 gcgacggtgc ggcgttcggc ggcgccgtga acaacgggga ggtcacgcgc atcgtgttca
     1921 acggccaacg tgacgcgatc gcgggccact cggtggtgta caaccacgga gggagcacgc
     1981 tgtcgttcga gatcggaccc ggggggcgga tcttcttcag
//
LOCUS       DASUYD010000118         2691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_946937, whole genome shotgun sequence.
ACCESSION   DASUYD010000118 DASUYD010000000
VERSION     DASUYD010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2691
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_946937"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VF195_06465"
     CDS             complement(<1..1068)
                     /locus_tag="VF195_06465"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX6580496.1"
                     /translation="MTWESSAITITTLLPIVGALVIILVPRDRDRMVRGLGILFTGAA
                     LVLSIAIAIGFDYGTDGLQFVLDVDWIAAIGARFHVGIDGISMPLYVLTFLLSFLCAV
                     YTWRFVPEPGRTKAFLALMLLLETGMAGTFIAFDLILFFVFWEMVLVPMYFLIGIWGS
                     ANREYAAIKFFLYTLFGSVFMLLGFLALYFSGPDPHTFDLVKLTEFGAAGGFTRTFQL
                     IVFGAIALGFAIKVPMWPFHTWLPDAHTEAPTIGSVLLAGIMLKMGTYGFIRIALPVL
                     PEGAQAYAPWIGVLAAIAIVYAALACLAQRDLKRLIAFSSVGHMGFVMLGIATLTEIG
                     IQAAVFGMVAHGVITGMLFFCV"
     gene            complement(1078..>2691)
                     /locus_tag="VF195_06470"
     CDS             complement(1078..>2691)
                     /locus_tag="VF195_06470"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX6580497.1"
                     /translation="GDVRFTWFYVVLSLFTGAMLTVVIADNLLMLLVGWEIMGVCSYL
                     LIGHWYEEHENSSAAIKAFITTRIGDVPFLFGIFALIFATGFTTTNIAEIGETIAGPG
                     TSPALVTAAALLLFGGAIGKSAQFPLHVWLPDAMAGPTPVSALIHAATMVAAGVYLVG
                     RMFEVFVHADPFVLTIVSAIAAITTLGAAFLALVQDDIKRVLAYSTLSQLAYMVAGLS
                     LGPEGVTAGFFHLFTHAFFKALLFLGAGSVIHAVHSNNMSDMGGLKDEMPVTFWTFLI
                     GSAALAGVFPLAGFWSKDELLVGAWEHNQWLFYVLLAVAVMTAFYMTRCVLLTFFGRP
                     SGEGHPHESPAVMTGPLVGLAGASVIVGFLGAPQLGAVFGDWVYFEHPHEADFVFWVA
                     VVGTLAAAAGILGGYRLYRERRERDPLMAFPRAWNVLQHRYYIDDLYMRGIVYPIRDR
                     VSAGVNWTNQHVLDGVVNGAAWVTRGLATFVSWIDRTFVDGAVNAVGGLTGATGGLLK
                     YLQTGNVQWYAVLLFAGVVVLTIVFVQIA"
BASE COUNT          551 a          900 c          893 g          347 t
ORIGIN      
        1 gacgcagaag aacagcatcc ccgtgatcac gccgtgggcg accatgccga agaccgccgc
       61 ctggatcccg atctcggtga gggtcgcgat gccgagcatc acgaagccca tgtgcccgac
      121 cgaggagaac gcgatcagcc gtttcaggtc ccgctgcgcc agacacgcga gggcggcgta
      181 gacgatcgcg atcgccgcga ggaccccgat ccagggggcg taggcctgcg cgccctccgg
      241 gagcacgggc agggcgatcc ggatgaagcc gtacgtcccc atcttcagca tgatcccggc
      301 cagcagcacg gagccgatcg tgggtgcctc ggtatgggcg tccggcagcc acgtgtggaa
      361 cggccacatc gggaccttga tcgcgaaccc gagcgcgatc gccccgaaca cgatcaactg
      421 gaacgtgcgg gtgaagccgc ccgcggcccc gaactcggtg agcttgacga ggtcgaacgt
      481 atgcgggtcc ggaccgctga agtacagagc gaggaacccc agcaacatga acacggagcc
      541 gaagagcgtg tagaggaaga acttgatcgc cgcgtactcg cggttggccg acccccagat
      601 cccgatcagg aagtacatcg gaacgaggac catctcccag aacacgaaga acaggatcag
      661 gtcgaacgcg atgaacgtgc cggccatccc ggtctccagg agcagcatca gcgcgaggaa
      721 ggccttcgtg cgacccggct ccggcacgaa ccgccacgtg tagaccgcgc acaggaacga
      781 caggaggaac gtgaggacgt agagcggcat cgagatgccg tcgatgccga cgtggaagcg
      841 cgcgccgatc gcggcgatcc agtcgacgtc gagcacgaac tgcagcccgt ccgtcccgta
      901 gtcgaacccg atcgcgatcg cgatcgacag caccagcgcg gcgccggtga acaggatgcc
      961 gagacctcgc accatgcggt cgcggtccct cggtacgagg atgatcacca gcgcgccgac
     1021 gatcggcagc aacgtggtga tcgtgatcgc cgagctctcc caggtcatct cacactccta
     1081 cgcgatctgc acgaacacga tggtcaggac gacgacgccc gcgaacagca gcacggcgta
     1141 ccactgcacg ttcccggtct gcaggtactt caagaggcca ccggtggccc ccgtcaagcc
     1201 gccgacggcg ttcacggcgc cgtcgacgaa cgtgcggtcg atccagctga cgaacgtggc
     1261 cagcccgcgg gtcacccagg cggccccgtt gaccaccccg tcgagaacgt gttggttggt
     1321 ccagttcacg cccgcggaga cccggtcacg gatcgggtag acgatgccac gcatgtagag
     1381 atcgtcgatg tagtaccgat gctgcaacac gttccacgcg cgcgggaacg ccatcagcgg
     1441 atcacgctcg cgccgttccc ggtacagccg gtagccaccg aggatcccgg cggcggccgc
     1501 gagggtcccg acgacggcca cccagaacac gaaatccgcc tcatgcggat gctcgaagta
     1561 gacccagtcg ccgaacacgg cgccgagctg cggggctccg aggaacccca cgatcaccga
     1621 cgcgccggcg agacccacga gggggcccgt catcacggcc ggcgactcgt gcgggtggcc
     1681 ctcgcccgag ggtctgccga agaacgtgag caggacgcac cgggtcatgt agaacgcggt
     1741 catgaccgcc acggcgagga ggacgtagaa gagccactgg ttgtgctccc acgcgcccac
     1801 caggagctcg tccttcgacc agaagccggc gagggggaag accccggcca gcgcggcgga
     1861 gccgatcagg aacgtccaga acgtgacggg catctcgtcc ttcaacccac ccatgtcgct
     1921 catgttgttc gagtggaccg cgtggatcac cgagccggcg cccaggaaga ggagcgcctt
     1981 gaagaacgcg tgcgtgaaga ggtggaagaa gccggcggtc actccctcag ggccgagcga
     2041 gaggccggcc accatgtagg cgagctggga gagcgtcgag tacgcgagga cccgcttgat
     2101 gtcgtcctgc accagggcga ggaacgcggc gccgagcgtg gtgatcgccg cgatcgcgct
     2161 cacgatcgtc agcacgaacg gatccgcgtg cacgaagacc tcgaacatcc tccccacgag
     2221 gtagacaccg gcggccacca tcgtcgcggc gtggatcagt gccgagacgg gcgttggacc
     2281 cgccatcgcg tcgggcagcc agacgtgcag cgggaactgg gcggactttc cgatcgctcc
     2341 gccgaagagc aggagggccg ccgcggtcac gagcgcgggt gacgtccccg gcccggcgat
     2401 cgtctcgccg atctccgcga tgttcgtcgt ggtgaacccc gtcgcgaaga tcagcgcgaa
     2461 gatcccgaac aggaacggca cgtcgccgat ccgcgtggtg atgaacgcct tgatcgcggc
     2521 gctcgagttc tcgtgctcct cgtaccagtg cccgatcaac aggtaggaac agacgcccat
     2581 gatctcccag ccgaccagca gcatcagcag gttgtccgcg atgacgacgg tgagcatcgc
     2641 gcccgtgaac agggacagga cgacgtagaa ccacgtgaac cgcacgtccc c
//
LOCUS       DASUYD010000119         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_506135, whole genome shotgun sequence.
ACCESSION   DASUYD010000119 DASUYD010000000
VERSION     DASUYD010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506135"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF195_06475"
     CDS             <1..500
                     /locus_tag="VF195_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6580498.1"
                     /translation="GASGGVGTFLVQLGAAAGLRIAAVGSEPSHSLLRELGATWTLDY
                     RTRNVAEAAQEEVGGPVDAIVDLVGADVLQASLPAVRPHGSLAAIATPILDLDPVLDS
                     NLSFHGVLIENDRDRIRTLGRLLADGTIRSIVSAVFSPERASEAHQLLETGHSGGKIV
                     LQFRS"
     gene            complement(1171..1836)
                     /locus_tag="VF195_06480"
     CDS             complement(1171..1836)
                     /locus_tag="VF195_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012921891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580499.1"
                     /translation="MIGVLLADDQVLVRAGFRALLDAQDDIDVVGEAADGREAVRLAA
                     DLRPNVVLMDVRMPGMDGLTATRTIAGDRRLADVRVVILTTFGVDEYVFEALRSGASG
                     FLVKDTEPSELIQAVRVVAAGDALLSPAITKRLIEEFATHARDRRSSPELGQLTDRER
                     EVMALVAEGLSNEEIATRLFVSPATAKTHVSRAMVKLGARDRAQLVVMAYESGLVRPG
                     WLG"
     gene            complement(1874..2347)
                     /locus_tag="VF195_06485"
     CDS             complement(1874..2347)
                     /locus_tag="VF195_06485"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580500.1"
                     /translation="MFSDIVSSTERAAAIGDRRWRELLDRHDELIRDELARHGGRAIK
                     TTGDGFLALFDGPARAIRCSVAIRQALEAEGVEVRIGLHSGEVELRGEDVGGIAVNLG
                     ARVAATAAPGEVVVSSTVRDLVAGSGITFVDRGEHDLKGMPDRWRLFAIAESVSA"
     gene            complement(2420..2830)
                     /locus_tag="VF195_06490"
                     /pseudo
     CDS             complement(2420..2830)
                     /locus_tag="VF195_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010352972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(2827..>3061)
                     /locus_tag="VF195_06495"
     CDS             complement(2827..>3061)
                     /locus_tag="VF195_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580501.1"
                     /translation="HAGQATATVRIAFGERDLTVQVDDDGNGAALHAKSGTGKGILGM
                     RERVAALGGELEAGPRPGRGFRVRARLPLDGTG"
BASE COUNT          470 a         1107 c          998 g          486 t
ORIGIN      
        1 tcggtgcgag cggcggcgtc ggaacgttcc tggtgcagct cggcgccgcg gccggcctcc
       61 ggatcgccgc ggtcggcagc gagccctccc acagcttgct gcgcgaattg ggagcgacct
      121 ggacgctcga ctatcggacc cgtaacgtgg ccgaggccgc ccaggaggag gtgggcggac
      181 cggtcgatgc catcgtcgac ctggtcggcg cggacgtgct ccaggcaagc cttcccgccg
      241 tccgaccgca tggctccctc gccgccatcg cgacgccgat cctcgacctt gatccggtgc
      301 tggactcgaa cctctcgttc cacggcgtct tgatcgagaa cgatcgtgac cggattcgca
      361 cgctcggccg actccttgcg gacggcacga tccgctcgat cgtgtcggcg gtcttctcgc
      421 ctgagcgggc ctcggaagcg catcaactcc tggagaccgg ccacagcggc ggtaagatcg
      481 ttctccagtt tcggtcctga gccgacgcgt ctggtgggtc ctgccgcccg gaacgagcag
      541 acttctccga ggccgttcga ctccccgccc cgccaacggg aggcggacct cgatcgaatg
      601 cgctggccct gcgccatgca gggcaatcgc cgatcacgcg caccgaaagc taagatcgtg
      661 ccgaatgcca cgactggcac cagatggcgc gccatgaccg cccccacgcg acgcgcaaga
      721 gcgatggcgg catggaaccc ctgcgcacac tcaaacgccg actatccgat gtcgtctacc
      781 gggccccgat caccgacgcg cgaggaacgt cgggataaca tcgcgttgtg tcccacacaa
      841 tagacctcgt gttccccgcc caggagagca tcgttgctga ccgctcggga ggaccatggt
      901 cgtagtggcc gttgaatggg cttggctcga ggctggagtc gatgcgaact aggtcgggaa
      961 ccgtggcgtc cagccggcgc tgaccgccgc gtcgaagatc cccccgatgg cggcgtccga
     1021 ggggatcgca cgttgccgtg tgataccggc tgcgtgctgc cttcccctgt tcgcgaggtg
     1081 ctacccgatc tcgatgcgag cagccgacgt ggatctagga aggatggccc accacgctga
     1141 tcgtcactcg cctgcggatc gtgcccaaca tcagccgagc caaccgggcc gcaccaaccc
     1201 cgactcgtag gccatcacca cgagttgcgc gcggtcccgg gctccgagct tcaccatggc
     1261 gcgactaacg tgggtcttgg ccgtcgccgg gctcacgaag agccgcgttg cgatttcctc
     1321 gttggataac ccctcggcga cgagcgccat gacctcacgc tcccggtcgg tgagctgacc
     1381 gagttccggg ctcgatcgcc ggtccctcgc gtgggtcgcg aactcctcga tcaggcgctt
     1441 cgtgattgcc ggggacagca gcgcgtctcc cgcggccacc actctgacgg cctggatgag
     1501 ctcggacggt tccgtgtcct tgacgaggaa tccgctcgcg ccggagcgga gcgcctcaaa
     1561 gacgtattcg tcgacgccga acgtcgtcag gatcacgact cggacgtccg cgaggcgccg
     1621 gtcgccggcg atcgttcggg tggccgtcag gccgtccatc cccggcatcc ggacgtccat
     1681 gagcaccacg tttggcctca ggtcggcggc caagcgcacc gcctcccggc cgtccgcggc
     1741 ctcgcctact acgtcgatgt cgtcctgggc gtcgagcaga gcgcggaagc ccgcccgcac
     1801 cagcacctgg tcgtcggcga ggaggacgcc gatcacggag cgcgccaacg tccacaggcc
     1861 acggtctgcc ccttcatgcc gacacgctct ccgcgattgc gaacagtcgc caccggtcgg
     1921 gcatgccctt gaggtcatgc tctcctcgat cgacgaacgt gatccccgag ccggcgacga
     1981 ggtcgcggac cgtgctcgag accacgacct ctccgggcgc ggccgtcgcc gcgacgcgtg
     2041 ccccgaggtt gacggcgatg cctccgacat cctcgcctcg cagctcgacc tcgcccgagt
     2101 gcagcccgat ccgaacctcg acgccttcgg cttcgagcgc ctgccggatc gccacggagc
     2161 atcggatcgc gcgtgccggt ccatcgaaca gggcgaggaa cccgtcgccg gtcgtcttga
     2221 tcgctcgtcc cccgtgccga gcgagctcgt cgcggatgag ctcgtcgtgg cggtcgagga
     2281 gttctcgcca cctccggtcg ccgatcgccg cggcgcgttc cgtggacgag acgatgtcgc
     2341 tgaacatcac ggtggcgagc acgcggtccg cgtccgatcc ggcggggagg gcgtgtccac
     2401 tgcccgggtc ggtgaacgcc gagatgagcc tacgcgtcac ccccggggac aacagcgcat
     2461 cgccccgtgc cacggcccgc acgccttgga cgagctggct cggatcggtg tccttgacaa
     2521 ggaacccgct cgcgccgacg cgaagcgcct cgaagacgta ctcgtcgaca tcgaacgtgg
     2581 tgaggatgac gatgcggacc cggtcgaggc gctggtcttg gacgatcctc ttcgtggctg
     2641 ccagcccatc ggtgccgggc attcggatgt ccatgagaac gacgtccggc gcgagctgcc
     2701 cggccaggcg gatggcctcg tcgccgtcgt tcgcctcacc aaccacctcg atgtcctctt
     2761 gggcatcgag gagggcgcgg aaccccgcgc gcaccaacgg ctggtcgtcg gctacaagga
     2821 cgcggatcat cccgtgccgt ccagggggag tcgggctcgc acacggaagc ctctccccgg
     2881 ccgaggaccc gcctcgagct caccgcccag ggcggccacg cgctcgcgca tgccgaggat
     2941 ccccttaccc gtcccggact tcgcgtgcag agcggcgccg ttcccatcgt cgtcgacctg
     3001 aacggtcagg tcgcgctctc caaacgcgat ccggaccgtc gccgtggcct gcccggcgtg
     3061 c
//
LOCUS       DASUYD010000120         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_507149, whole genome shotgun sequence.
ACCESSION   DASUYD010000120 DASUYD010000000
VERSION     DASUYD010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507149"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VF195_06500"
     CDS             <1..315
                     /locus_tag="VF195_06500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580502.1"
                     /translation="RVTVSVGVGADDVVIRVRDRGSGIDPDQRDRIFDRFYQVDQSAT
                     RQVGGVGMGLYICQRAAERLGARIWLDRSDASGSVFAVRLPTAHVAERQTADIVSVVA
                     SV"
     gene            1704..2342
                     /locus_tag="VF195_06505"
     CDS             1704..2342
                     /locus_tag="VF195_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580503.1"
                     /translation="MSRMIVSALFASALVACQGSAEVREAETAPEPTSGAVPWPAPSD
                     PLDLAVAAGLEPETHEFLDYHVHAHLDVFVNGSPVEVPAGIGIDITDPAVHTTPSDLG
                     PTYGGIDPPCAQPCISPLHTHDATGVLHTESAVDDPNSLGQFFTEWGVALTPECVGGY
                     CSTDASIQVFVDGEAFNGDPADIELADQREIAIVIGSPPEEIPSEFPDAAGT"
     gene            complement(2851..5451)
                     /locus_tag="VF195_06510"
     CDS             complement(2851..5451)
                     /locus_tag="VF195_06510"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEX6580504.1"
                     /translation="MEFRILGPLEVSVGDGAIKLGGPKQRAVLAHLILRANRPVPIDL
                     LIDGLWGEEPPETARNTLQTYVYRLRRVLGDDRISSEAGGYELRADAAEIDAAQFEAM
                     VKSAKVDLPSDPSKAAAGLSEALALWRGAPLADLSNEPSLRGEIARLEELHLSATEHR
                     IAAEIELGGHSTVVSELDALTARYPLRERMWANLMLALYRLGRQAEALAAYQRARQVL
                     ADELGTEPSRELQRLNEQILRRDPSLGLSEPARQGVRPSRVDLQPGTEVASYRIVHTL
                     GRGGMSVVYLAEHDWLQRKVALKVLAPQLADDERFRERFVRESRLAASLDHPNVIPIY
                     EAGASGGDLFIAMRFVEGSDLRTLLHEEGALEPARAIGILRQVASALDAAHEQGLVHR
                     DVKPANVLLARPRSSEPVEHVYLSDFGLTKRSASDSAVTGTGQFVGTLDYASPEQFKG
                     ETPDARTDVYSLGCVLFECLTGRPPFRSENDAGLMYAHLQQPAPPVTSERADLPHEID
                     RVVGRAMAKSPDDRPRSAGELADGAARALGAELPPREALLIDPGGGTYNPYQRLLAQG
                     IDRAISEFGLDAEKVPTAFDLMDQELRRLSEDGVDLILVPSIFVDVNAVAAEFPDTHY
                     VAIDFTGRPHPSLPNVSYVCFAHNEGSFLAGAAAALESRTGRIGFIGGMDIPMMRDEF
                     QAGYEAGARAVDPSIEVRSTYLTPYFDFSGFGSPAYAFDVATRMYRWGADVIYHAASA
                     SGRGVFQAAAEIQGRHVWAIGVDVDQYQEFATMDPEDAPPELHIAAWPSHVLTSMVKR
                     VDVAVYEVLRDYPRGIFMSGVRQFGLADGGVDIAYSGGFIDDIRPQIEDLRAQIIAGD
                     VVVPSKPAKA"
     gene            complement(5629..>6013)
                     /locus_tag="VF195_06515"
     CDS             complement(5629..>6013)
                     /locus_tag="VF195_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020576182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6580505.1"
                     /translation="AMNDELVSDSLVYRYDPAASPDGLAGSEGTFSICTFWYVDALAR
                     SGRLEEARMTFEKMLTYANHVGLFSEEIGPTGEQLGNFPQAFTHLSLINAAMNLDFQL
                     DHGPGWVDLAPGALRRAAGTNASIG"
BASE COUNT          994 a         2034 c         1998 g          987 t
ORIGIN      
        1 cgggtcaccg tctccgtcgg cgtcggcgcc gatgacgtcg ttatcagagt gcgggaccga
       61 ggatcgggga tcgatcccga ccaacgagac aggatcttcg accgcttcta ccaggtcgat
      121 cagtccgcca ctcggcaagt cggcggtgtc gggatgggac tctatatctg ccagcgcgcg
      181 gcggaacgac tcggggcacg tatctggctc gaccgatccg atgcctccgg atcggtcttc
      241 gccgttcgcc ttccgaccgc ccatgtcgcc gagcgccaga ctgccgacat cgtgagcgtc
      301 gtcgcaagcg tttgactcca caaagctgcg gtctggtcca tcaatggagg aagcccacct
      361 gcgaggggcg aggcgggcgt tcctccgatg ttgccagacc gatgtagaac ccgcccccta
      421 cagcggacaa acggactgcc cgcgccgcac agcgagccgg ctgtcaaccg ggaaggaact
      481 cgagcccgct cctgcaacga tccgcccgca tgagcgttgc aggatcgcag cgtcctcacc
      541 cgagaatcgt cgtgaaccaa gggcccgccc gctccgaggt ccctcagggg tttgagggag
      601 ccccgccggg ggacctcacc catattggaa tcatttggta gcttcggcgg ccgaggctcg
      661 actggccgca acgacctttc gattcacctg gggaagggcc gatgagcata ccgtccaaca
      721 actctgcgag gcgatgggca ggcgaacttc gggcgtggag aggctctcgt tggcagcagt
      781 tccgtgcctg gcagatggat cgacgggcgt ggggaggcta ctggtcgcac cggtttcgtt
      841 cctggctgac gaacggtcgg gatcgatggg cgtgggacga cttcaggtgg caccggttcc
      901 gttcctggct gacgtacggt cgggccaata agttcgtctt cgcgaccagc gtcatcctcg
      961 gctcggtcgt ccttgttgga ttgctcacta ccggacgagg aagtgccccg agactgttgg
     1021 gcgaagcctt cacgatcggg gtgacggacg agggagtcac gtacgttgcg ttagaccccg
     1081 aagggccggg ggaggcgcaa ggtgaggcag caaaggacga ggccagcctt gggggcggcc
     1141 cctcgccggt tgcgctcttc tccgtagacg cgagcggggg tgtcctgtct ctcgtcggcg
     1201 cagagggccc gttgatgggc aacgatcccg agcctcagcc cggtccaaat cccggccctg
     1261 gtccgtcacc cgttccgtcg ccaggaccct ctccctcccc gacgccgccc ccaacaagga
     1321 ccgaaccacc acctacgacc accgaaccac cacctacgac caccgaacca ccacctacga
     1381 ccaccgagcc gccgtccacg accaccgagc cgccgcctcc tacgacgact gagccgccgc
     1441 ctcccacgac gaccgaaccg ccgcctccga ccactgaacc gccgtctccg aacaccgaag
     1501 cgccggaacc gtcgagttga tgagaaccaa gaccaaccgc accttcgccg atcgctgatc
     1561 ggctgcgagg aggaccggac gctacaggcg gtgatcgtcg gcatgctgcg ggacggcgac
     1621 aaggctccct tcgatcgttg cgcggggagc ctacgccgca cggcgagccg gcggtgcata
     1681 ctgcccggat ggatgcgttg cgcgtgagca gaatgatcgt gtccgcgctg ttcgcttcgg
     1741 cgctggtcgc atgccaaggc tcagctgagg ttcgagaagc ggagaccgcc cccgagccga
     1801 cgtcgggtgc agtgccttgg ccggcgccat cggacccgct cgatctcgcc gtcgccgccg
     1861 gcctcgagcc cgagacccac gagttcctcg actaccacgt ccatgcgcat ctcgacgtct
     1921 tcgtcaacgg atcacccgtc gaggtgccgg cgggtatcgg catcgacatc acggaccccg
     1981 cagtccacac gacgccgagc gacctcggac cgacctatgg gggcatcgat cctccctgcg
     2041 cacagccctg tatctcgccg ctgcacactc acgacgccac tggggtcttg cacaccgaat
     2101 ccgcggtcga tgatccgaac tcactcgggc agttcttcac ggagtggggt gtggcgctca
     2161 cccccgaatg cgtcggcggc tactgttcga ccgatgcgtc gattcaggtg ttcgtggacg
     2221 gggaagcgtt caacggagat cccgccgaca tcgaacttgc ggaccagcgg gagatcgcca
     2281 tcgtgatagg ttcgccgccc gaggagatcc cttccgagtt ccccgacgcc gcgggcacct
     2341 agtgcaggct tgcattttgc cgaatgattg gccacgacga gttcaaaccg ggagcccgcg
     2401 ccgcacggca aaccgacggt ttaggtcttt cgacggatgc caaggtgcct cttcagcgga
     2461 tgcctcgata ccccaggtgg cttacgctgg ccgcatgaga ccgcgagtcg aaccgctcga
     2521 actctccggc aaagaaggct ccacaagggt ctactgcgcg acctgtgggc actcggaata
     2581 tgttcatgcg gacaacagcg atcgccggtg tttgtacacc gtgtgtgatt gcaactgttt
     2641 cattgcgggt gcggtgcctg acctctcggg tcaagtcctc cccgcctagg atcgtatcgg
     2701 gccatcgggc gcaccgggag ccagagtcgc acggcacgtg cagcctcgta gggtcgtgag
     2761 acgccgatag ggttgcctcc aggcctcgat atccaccgcg tgacccccgg gggaggccgg
     2821 ttctggacgt cctccgcagg atgcgcgggc tcaagccttg gcgggtttcg acgggacgac
     2881 gacatcgccc gcgatgatct gcgctcgcag gtcctcgatc tgcgggcgga tgtcgtcgat
     2941 gaatccgccg ctgtaggcga tgtcgacgcc gccgtcggcg agaccgaact gccgaacccc
     3001 cgacatgaag atgccccgcg ggtaatctcg gagcacctca tagacggcga cgtccacccg
     3061 cttcaccatc gaggtgagca cgtgggacgg ccaggccgcg atgtgaagct ccggcggcgc
     3121 gtcctcgggg tccatcgtcg cgaactcctg gtattgatcg acgtccaccc cgatcgccca
     3181 cacgtgcctg ccctggatct cggcggccgc ctggaacaca ccccttcccg aggcgctggc
     3241 ggcatggtag atgacgtcgg cgccccaccg atacatgcgc gtggcaacgt caaacgcgta
     3301 cgccggggag ccgaaaccgc tgaagtcgaa gtacggggtc aggtatgtcg accgcacctc
     3361 gatgctcgga tcgaccgccc gggcaccggc ctcgtagccg gcctggaact cgtcgcgcat
     3421 catggggatg tccatgccac cgatgaaccc gatccgcccc gtccggctct ccagggccgc
     3481 tgcggcgccc gccaggaacg agccctcgtt gtgggcgaag cagacgtagg agacgttggg
     3541 aaggcttggg tggggtcggc cggtgaagtc gatcgccacg tagtgcgtgt cggggaactc
     3601 tgcggccacc gcgttcacgt cgacgaagat cgatgggacg aggatcaggt cgacaccgtc
     3661 ttcggagagc cggcgcaatt cctgatccat gagatcaaag gcagtgggga ccttctccgc
     3721 gtcgaggccg aactcggaga tggcgcggtc gatgccctga gcgagtaacc gctggtaggg
     3781 gttgtaggtg cccccaccgg ggtcgatcaa cagtgcttcc cgcggcggca gctccgcgcc
     3841 gagggctcga gcggcgccgt cggcgagttc gcccgccgag cgtggacggt cgtctggcga
     3901 cttcgccatc gcgcgcccga ccacgcggtc gatctcgtgc ggcaggtcgg cccgctcact
     3961 cgtcacggga ggtgccggct gctgcaggtg ggcgtacatc aggccggcgt cgttctccga
     4021 tcggaacgga ggccggccgg tcaggcactc gaacaacacg catccgagcg agtacacgtc
     4081 ggtccgcgcg tcgggcgtct cgcccttgaa ctgctcgggc gacgcgtagt cgagcgtgcc
     4141 cacgaactgt cccgttccgg tgacggcgga atcgctcgcc gaacgcttgg tcagaccgaa
     4201 gtccgagagg tagacgtgct cgacgggctc ggagctgcgc ggtcgcgcta gcagcacgtt
     4261 cgccggcttg acgtcgcggt gcacgagccc ctgttcgtgc gccgcgtcca gggcggacgc
     4321 gacctgacgg aggatcccga tcgcccgtgc aggctccagc gctccctcct cgtgaagcag
     4381 cgtccgaagg tccgagccct ccacgaaccg catcgcgatg aacaggtctc cgcccgaggc
     4441 gcctgcttcg tagatcggga tcacgttggg gtgatcgagc gaggccgcaa gccgagactc
     4501 gcggacgaat cgctcgcgga accgctcgtc atcggccagc tgcggagcca ggaccttcaa
     4561 cgcgactttc cgctggagcc agtcgtgctc ggccaggtag accacgctca tgccgccgcg
     4621 acccagggtg tggacgatgc ggtagctggc gacctccgtg ccgggttgca ggtccacgcg
     4681 gctcggtcgg acgccctgcc gcgcgggttc gctgaggccg agcgacggat cgcgccggag
     4741 gatctgctcg ttgaggcgtt ggagctcccg tgagggctcc gtgccgagct cgtcggccaa
     4801 cacctgccgg gcccgttggt acgccgcgag cgcctcggct tgacggccga gccggtacaa
     4861 cgcgagcatc aggttggccc acatccgttc gcggagtggg taccgggcgg tgagcgcgtc
     4921 gagctcggag acaacggtcg agtgcccgcc caactcgatc tccgcggcga tgcggtgctc
     4981 ggtggccgac aggtggagtt cctccagtct cgcgatctcg ccccggagcg agggctcgtt
     5041 cgacaggtcg gcgagcgggg cgccgcgcca gagcgcgagg gcctccgaga gtcccgcggc
     5101 ggccttcgag gggtcggagg ggaggtcgac cttggcggac ttgaccatgg cctcgaattg
     5161 cgcggcgtcg atctcggcgg catcggctcg gagctcgtag ccgccggcct ccgaggagat
     5221 ccgatcgtcg ccgagcaccc gccgcagccg gtacacgtac gtctgcaggg tgttcctggc
     5281 cgtctcgggc ggctcttccc cccacaggcc gtcgatcaac agatcgatgg ggacagggcg
     5341 attcgcccgc aggatcaggt gggcgagcac cgcccgctgc ttgggtcccc ccagcttgat
     5401 cgccccgtcg ccgaccgaga cctcgagcgg cccgaggatg cggaactcca tggcgtccga
     5461 accctacgcc gagtcccggg ggcatgggaa ggcacggtca ggaccccctc tgccgccttt
     5521 ccgccgcctc ggcaaacaga cttccggggc tgctcttctc aggccgcttg cctctggatg
     5581 acccagcgcc cgagacttgc gccccagcct ttctggttac gacgggggtc acccaatgga
     5641 ggcgttcgtc cctgctgctc gtctgagcgc cccgggcgcc agatcgaccc atcccggccc
     5701 gtggtcgagt tggaaatcga ggttcatcgc ggcgttgatc agcgacaggt gggtgaaggc
     5761 ctgggggaag ttccccagct gctctcccgt cggcccgatc tcctcggaga acagcccgac
     5821 gtggttggca tatgtcagca tcttctcgaa cgtcatccga gcctcttcga gccggcccga
     5881 gcgagccagg gcgtcgacgt accagaacgt gcagatcgag aacgttccct cggagccggc
     5941 cagaccgtcc ggggacgcgg cgggatcgta gcggtagacc aggctgtccg agaccagctc
     6001 gtcgttcatc gca
//
LOCUS       DASUYD010000121         5575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1168848, whole genome shotgun sequence.
ACCESSION   DASUYD010000121 DASUYD010000000
VERSION     DASUYD010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168848"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF195_06520"
     CDS             <1..241
                     /locus_tag="VF195_06520"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6580506.1"
                     /translation="PFPALNNVKWLAFDDGEQIGKVTSAIHSPRLGKNIGYCWLPTER
                     SSAGTTVSVETEWGTRTATVAPMPFVDPDQTIPVS"
     gene            372..1553
                     /locus_tag="VF195_06525"
     CDS             372..1553
                     /locus_tag="VF195_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6580507.1"
                     /translation="MVNERPEMLAKSATLYFGPWYRQSPFFRSTLRAGCTAYDIYNHM
                     YLPGYFDDPEVEYHLLATGVTMWDVGVERTVQIAGPDADRLIDMITCRDLSTCAVKQG
                     KYMIVTAPDGGIVNDPVLLHVAENTWWMQLADSDAGLYAMGVAATSGLDAEVSFPDVH
                     PMQVQGPLSAKTLEKLVGPAIYDIKYYWVDEFEIRGIPVLISRTGWTAIPGFEVNLLD
                     GSRGDELWNAVAEAGEEFGIKPIAPCEARRIEAGIFNYGSDMKIDDTPLHVMGLERLV
                     EEQPQDYIGKEALEHLKREGVDRKLVGIELEGDQLRAEMSEYWPVHHGGTQVGHMTDA
                     VWSPGLARNIGYVWVPFDLSAPGTALDVETEDGDHLVGRTAAIPFVDPRKERPAGSLL
                     E"
     gene            1626..2750
                     /locus_tag="VF195_06530"
     CDS             1626..2750
                     /locus_tag="VF195_06530"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6580508.1"
                     /translation="MKSATTSRRALPAVAYVALLLAGCTATLGGTASPPATTPPGPSL
                     HLGKEPYAAVATRVLPSVVNVTTDVYQASPSGAPEEGQGIGTGFIVRANGVVVTNCHV
                     VQGASRITVFTSEDEPTRYDARVIGGDCEHDLAVLKVDAEGLPTIRFGASANLRLGQQ
                     VVAIGYALALEGGPSVTAGIVSSLDRTIRVQDPRCTVCSNGVRTYSEVIQTDAAINHG
                     NSGGPLVDMAGRVVGINSAGSDNAENIGFAIAIDSVQPAIESAISDPLRATAYLGIST
                     RSVTADLAFQMDLPVESGALVLATLANGPAADAGMREGDVIIDIDGEAIEVAEDLGRV
                     LDALAPGDEVDVRVVRSDGEERTHRVTLGTKPLPTEFLEP"
     gene            2847..3632
                     /locus_tag="VF195_06535"
     CDS             2847..3632
                     /locus_tag="VF195_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017975847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N1)-methyltransferase"
                     /protein_id="HEX6580509.1"
                     /translation="MSAPFEAGEKVLLIDQRDRTHLFSLRAGATYHTHAGTLAHDDVI
                     GRPEGTRVTTARGMVFAAFRPRFADFVLKMPRGAQVVYPKDLGPIVVYADVYPGARVL
                     EAGTGSGALTIALCRAVGEEGRVVSYELREEHRAQAQANVESFFGKIPDRLDMRAGDV
                     TEAAAGEERFDRVVLDLPEPWSPLESLHAVLEPGAVLCAYLPTTIQVHQLVLALPRSG
                     FLHIETFEVLKRGWHVTERSVRPDHRMIGHTGFLTLARVGPED"
     gene            3761..5503
                     /gene="arc"
                     /locus_tag="VF195_06540"
     CDS             3761..5503
                     /gene="arc"
                     /locus_tag="VF195_06540"
                     /EC_number="5.6.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000502 - proteasome complex [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome ATPase"
                     /protein_id="HEX6580510.1"
                     /translation="MADRREQAEQREKQVHELQTQISFLEEEVGLLRRRLTNAPRQVQ
                     LLEEKLVETREALARATGQNEKLADALRDEKQRIEALAEEVEKLSQPPATFGVFVGSN
                     DDGSVDVVTAGRKLRVMPAPDLDADKLVSGAQVILNDALNVVEVLDPDGTGQVVKVKD
                     KLGTDRAVVVSRGDEEHVAYLAGSLLGTVVRAGDPVLYDARSGVATELLPREEVEELV
                     LEEIPDITYEDIGGLEGQIEAIRDAVELPFLYAELFQEHQLEAPKGVLLYGPPGCGKT
                     LIAKAVAKSLADKVRERTGREDARSYFLNVKGPELLNKYVGETERQIREIFQRAKERS
                     EEGLPVIVFFDEMDSIFRTRGTGISSDVESTIVPQLLSELDGVETLKNVIVIGASNRE
                     DLIDPAILRPGRLDVKIKIERPTAEQAKDVMSKYLHPVVPIHPDEVDRHSGDVQVAVA
                     RMIERTVEQMYEPSERNRFLEVTYASGDKEILYFKDFNSGAMIENIVRRAKKDAIKRF
                     LSKGEKGIKTEDLLTAIRDEFKENEDLPNTTNPDDWARISGKKGERIVYVRTLLGDEG
                     DGRQVERVSPGQYL"
BASE COUNT          988 a         1851 c         1916 g          820 t
ORIGIN      
        1 gccgttcccg gcgctgaaca acgtcaagtg gcttgccttc gacgacggcg agcagatcgg
       61 caaggtgacg tcggcgatcc actcgccgag gctcggcaag aacatcggct actgctggct
      121 ccccaccgag cggtcgtcgg cgggcaccac ggtgtcggtc gagaccgagt ggggcacccg
      181 gaccgccacg gtggcaccga tgccgttcgt cgacccggac cagacgatcc cggtctcgta
      241 gcgcgccggc cgcaggaaac ctaaccgccg ttcaaccaca tcgctcctgt tcaggggacg
      301 tgcggcgact tgcgcgcacg ctcttcttga acgatgattc agcctgttcc cggcccgagc
      361 gaggaggaac gatggtcaac gaacgacccg agatgctcgc gaagagcgcg accctgtact
      421 tcggcccttg gtaccgccag tcgccgttct tccggtcgac gctgcgggcg ggctgcaccg
      481 cctacgacat ctacaaccac atgtatctgc ccggctactt cgacgatccc gaggtggagt
      541 accacctgct cgcgaccggc gtgacgatgt gggacgtggg cgtggagcgg accgtccaga
      601 tcgccgggcc ggacgccgac cggctgatcg acatgatcac gtgccgcgat ctctcgacgt
      661 gcgccgtgaa gcagggcaag tacatgatcg tcaccgcacc cgacggaggg atcgtgaacg
      721 acccggtcct gctgcacgtc gccgagaaca cgtggtggat gcagctggcc gactccgatg
      781 cgggcctgta cgcgatgggg gtcgcggcca catcgggcct cgacgccgag gtctcgttcc
      841 cggacgtcca tccgatgcag gtccagggcc cgctctcggc caagacgctc gagaagctcg
      901 tcggtcccgc gatctacgac atcaagtact actgggtgga cgagttcgag atccggggca
      961 tccctgtcct catcagccgg acggggtgga ccgcgatccc gggcttcgag gtgaacctcc
     1021 tcgatggcag caggggagac gagctctgga acgccgtcgc cgaggctggc gaggagttcg
     1081 ggatcaagcc catcgcgccg tgcgaggccc gccggatcga ggccgggatc ttcaactacg
     1141 gctccgacat gaagatcgat gatacgccgc tccacgtgat ggggctcgag cgcctcgtcg
     1201 aggagcagcc acaggactac atcgggaaag aggccctcga acatctcaag cgggaagggg
     1261 tggatcggaa gctcgtcggg atcgagctcg agggcgacca gctccgagcg gagatgtccg
     1321 agtactggcc cgttcatcac gggggcacac aggtcggcca catgaccgac gccgtctggt
     1381 cgcccggcct ggccaggaac atcggctacg tctgggtgcc gttcgatctc tccgcgccgg
     1441 ggacagcgct cgacgtcgag acggaggacg gggaccatct cgtggggcga accgccgcca
     1501 tcccgttcgt cgatcctcgg aaggagcggc ccgccggctc gctgctcgag taacctcgat
     1561 gctcagcgcc tccgacggag gtagcggaag ccgaaccctg gcaggaccgg gagcgaaccc
     1621 ctcgcgtgaa gtcagccacg acatcccgac gagcgctgcc ggcggtcgcg tacgtggccc
     1681 tcctgctcgc cggctgcacg gcgacgctcg ggggcacggc atcccctccc gcgacgacgc
     1741 cacccggccc gtcgttgcat ctcgggaagg agccctacgc cgcggtcgcg acgagggtcc
     1801 tcccatccgt ggtcaacgtg acgacggacg tctatcaggc ctcgccgagc ggcgctcccg
     1861 aggagggcca ggggatcggg accggcttca tcgtccgagc gaacggtgtc gtggtcacca
     1921 actgtcacgt cgtacagggg gcctctcgga tcacggtgtt cacgtccgag gacgaaccca
     1981 cgcgctacga cgcccgcgtg atcggtggcg attgcgagca tgatctcgcc gtcctgaagg
     2041 tggacgccga gggtctcccg acgatccggt tcggagcgag cgcgaacctc cggctcggcc
     2101 agcaggtcgt ggcgatcggc tacgccctgg cgctcgaggg agggccatcc gtaacggcgg
     2161 gcatcgtctc ctccctcgac cgaacgatcc gcgtgcaaga tccacgatgc accgtgtgct
     2221 cgaacggcgt tcgcacctac agcgaggtga tccagacgga cgcggcgatc aaccacggga
     2281 actccggagg accgctggtc gacatggccg gccgggtcgt ggggatcaat tcggccggca
     2341 gcgacaacgc cgagaacatc ggcttcgcga tcgcgatcga ctccgtccag ccggcgatcg
     2401 agtcagcgat ctcggacccg ttgcgagcca ccgcgtatct cggcatctcg accaggagcg
     2461 tgacggccga cctcgcgttc cagatggacc tgccggtcga gagcggggcg ctcgtcctgg
     2521 ccaccctcgc gaacggcccc gcggccgacg cggggatgcg tgaaggggac gtgatcatcg
     2581 atatcgacgg cgaagcgatc gaggtggccg aggatctcgg acgcgtgttg gacgctctcg
     2641 ctccgggcga cgaggtcgac gtccgcgtgg tccgctccga cggagaggaa cgcacccacc
     2701 gggtgacgct gggaacgaag cctctcccca cggagttcct ggagccctga cctccgtcga
     2761 cccccctgcg gccacgggcc ggggagcccc agcggtctgc gctagcctct gcacgtgagg
     2821 cacaagtgcg acgacggagc ccatcgatga gcgccccgtt cgaagcgggc gagaaggtcc
     2881 tgctgatcga tcaacgggac cgcacgcatc tgttctcgct ccgggcagga gccacgtacc
     2941 acacgcacgc cggcaccctg gcccacgacg acgtgatcgg gcggcccgag ggaacgcggg
     3001 tcaccaccgc ccgcggcatg gtcttcgcgg cgttccggcc gcggttcgcc gatttcgtgc
     3061 tcaagatgcc ccgcggcgcg caggtcgtct acccgaagga cctcggaccg atcgtcgtgt
     3121 acgccgacgt ctatccgggc gcacgcgtgc tggaggcggg cacgggttcg ggcgcgctga
     3181 cgatcgccct ctgccgggcg gtcggggagg agggccgcgt cgtctcctac gagctgcggg
     3241 aggagcatcg ggcacaggcc caggcgaacg tcgagtcctt cttcgggaag atcccggacc
     3301 ggctggacat gcgcgccggc gacgtgaccg aggcggccgc cggcgaggag cggttcgatc
     3361 gggtcgtcct ggatctcccc gagccgtggt cgccactcga gtcgctgcac gcggtgctgg
     3421 agccgggcgc cgtcctgtgc gcctaccttc ccaccacgat ccaggtccac cagctcgtcc
     3481 tggcgctgcc ccggtctggc ttcctccaca tcgagacgtt cgaagtgctg aagcgagggt
     3541 ggcacgtgac ggaacggagc gtgcggccgg accaccggat gatcggtcac accggcttcc
     3601 tgaccctggc ccgcgtcgga ccggaggact agcatcgagt aagtacccgg ttccaggcat
     3661 cggcctcgtt cgcctggctg ccgtatctac agaaaaggac cccctgaggg gtcaagatgc
     3721 tgccagtacc atcgatgact cgggtgggag gtgagaacgg atggcggatc gccgcgagca
     3781 ggcggagcaa cgtgagaagc aggtccacga gctgcagacg cagatttcct tcctcgagga
     3841 agaggtgggg ctgctgcgac gccgcctgac caacgccccc cgccaggtcc agctcctcga
     3901 ggagaagctc gtcgagacga gggaggcgct cgcccgcgcc accggccaga acgagaagct
     3961 cgcggacgcc ctgcgcgacg agaagcagcg catcgaggcc ctcgcagagg aggtcgagaa
     4021 gctctcccag ccgcccgcca ccttcggggt gttcgtcggg agcaacgacg acggatccgt
     4081 cgatgtcgtg accgccggcc ggaagttgcg cgtgatgccg gccccggacc tcgatgccga
     4141 caagctggtg tccggggcgc aggtgatcct gaacgacgcc ctcaacgtgg tggaggtact
     4201 cgaccccgac ggcaccggcc aggtggtcaa ggtgaaggac aagctcggca ccgaccgagc
     4261 ggtggtcgtg agccggggtg acgaggagca cgtggcgtat ctggccgggt cgctcctcgg
     4321 gaccgtcgtg cgcgccggcg acccggtcct gtacgacgcg cgctccggcg tggccacgga
     4381 actcctcccc agggaagagg tcgaagagct cgtcctggag gagatccccg acatcaccta
     4441 cgaggacatc ggagggctcg aaggacagat cgaggcgatc cgcgacgccg tcgagctccc
     4501 cttcctctac gcggagctct tccaggagca tcagctcgag gccccgaagg gcgtgctgct
     4561 ctacggaccg ccgggctgcg gtaagacgct gatcgccaag gccgtcgcca agtcgctggc
     4621 ggacaaggtg cgcgagcgga ccggccggga ggacgcccgc agctacttcc tgaacgtgaa
     4681 gggcccggag ctcctcaaca agtacgtcgg cgagaccgag cggcagatcc gggagatctt
     4741 ccagcgcgcc aaggagcgta gcgaggaagg gctccccgtg atcgtgttct tcgacgagat
     4801 ggactcgatc ttccgcacgc gagggacagg catcagctcg gacgtggagt cgacgatcgt
     4861 gccgcagctg ctctccgagc tggacggtgt cgagaccctc aagaacgtga tcgtgatcgg
     4921 cgcgtccaac cgcgaggacc tgatcgaccc cgcgatcctg cggcccgggc gcctcgacgt
     4981 caagatcaag atcgagcgac cgaccgccga gcaggccaag gacgtcatgt ccaagtacct
     5041 gcatcccgtg gtgccgatcc atcccgatga ggttgaccgg cactcgggcg atgtccaggt
     5101 ggccgtcgcc cggatgatcg agcgaaccgt cgagcagatg tatgagccct cggagcgcaa
     5161 ccggttcctc gaggtcacgt acgcgtccgg cgacaaggag atcctctact tcaaagactt
     5221 caactcgggg gcgatgatcg agaacatcgt ccgccgcgcg aagaaggacg ccatcaagcg
     5281 attcctgtcc aagggcgaga agggcatcaa gaccgaggac ctcctgacgg cgatccggga
     5341 cgagttcaag gagaacgaag atctcccgaa caccaccaac cccgacgact gggcgaggat
     5401 ctcgggcaag aagggcgagc ggatcgtcta cgttcgcacc ctgctcggtg acgagggtga
     5461 cggtcgccag gtcgagcgcg tgagtcccgg ccaatatctg tagggtcgcc gggtggccat
     5521 cgcgaaggtc ctgggcaccg agacggagta cggcatcgcc gcggtcggtg tgccg
//
LOCUS       DASUYD010000122        14961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1169773, whole genome shotgun sequence.
ACCESSION   DASUYD010000122 DASUYD010000000
VERSION     DASUYD010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1169773"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VF195_06545"
     CDS             <1..254
                     /locus_tag="VF195_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580511.1"
                     /translation="RSTPAAVAQTGGWAAMDAMHDSPAMQRAHAQMPEELRAQCEAMH
                     EQMEQMMGAGGMMSGDMGSHHSGMAADGMMGTGRDVSAG"
     gene            300..533
                     /locus_tag="VF195_06550"
     CDS             300..533
                     /locus_tag="VF195_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007492734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6580512.1"
                     /translation="MPYGWHDGGWGVLWMILSWALIIAIVVLVVRAFSSGSSDRLDRT
                     NDPRAILDERFARGEISEDEYRGRTSVLEETRR"
     gene            complement(680..1684)
                     /locus_tag="VF195_06555"
     CDS             complement(680..1684)
                     /locus_tag="VF195_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HEX6580513.1"
                     /translation="MRLALAAGVFVSFALLPHLLRTSLLARGSPMVLAWFGVTSLLGI
                     GAGLFAVLAATVSPGPLPLVDLPRAVEICIGAAGRLLSHPLKHWPSILAAVLFLAAAV
                     RVIAAAVLVARDAKRARPPTSRFPGEERALREHLGIVPSSVRLLPFEGSVAFTTGLVR
                     PVTVVSDGLMRALAPKERSAVIAHERAHASRRHMAALSAARIVARAFGFVPGVLVSVE
                     LLVTALEAWADEQATTSVGDPLFVARALATTARLTFEQSAAVASITDGEIGYRIRQLT
                     ARRRPRMRRSLVVVLVLATIAMVLAQGAAWSAGQQALTRERLALALHDTCHPPHGPVS
                     "
     gene            complement(1681..2073)
                     /locus_tag="VF195_06560"
     CDS             complement(1681..2073)
                     /locus_tag="VF195_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018492542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX6580514.1"
                     /translation="MAKLIGELGPLEAEIMDHAWKRGDPVTVREVVDSVGVRNGLAYT
                     TVMTVMERLSTKGFLVRRKVGRAYSYKTRVSRDDYSAALVRSVLAASKDRRSVLLGFV
                     RSVKEDDLKELERLVKQAQREGRSGRRS"
     gene            2480..2800
                     /locus_tag="VF195_06565"
     CDS             2480..2800
                     /locus_tag="VF195_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580515.1"
                     /translation="MARANRRRTGPSRPRNKRRDIQARDTQASQQRAMQRKQITVRAY
                     RFRRALGWGLVGLGIVVGVSHWLSHIGFWGFASQGVMDLVAGYPMAAALVIAGSIVLG
                     RLSK"
     gene            complement(2940..3581)
                     /locus_tag="VF195_06570"
     CDS             complement(2940..3581)
                     /locus_tag="VF195_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6580516.1"
                     /translation="MTALALALVAGMLAFTSPCSLPLMPGYVSYVSGVAGDAGGSVAV
                     RSRVVGSALLFVSGFASVFTLLGASASAAGTFLLRNRILLTRVAAIFVIAMGLVAIGV
                     LRFSFLYRERRLDLRRIRTGPAGAIPLGMAFAIRWTPCIGPVLAGILTATASAQSAFR
                     GALLLFTFSIGMGIPFVLLALGYSRSLRAFEWLWRHGRAVERGGAWCSSRSAC"
     gene            3897..4433
                     /locus_tag="VF195_06575"
     CDS             3897..4433
                     /locus_tag="VF195_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011228871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6580517.1"
                     /translation="MVEEQLDEGDPTPPHRRGRLLLAVLVPVMAFALLLATGLGGDPR
                     ALPSELVRRPAPSFVLPRLSGGGSVHLRQLRGQVVVVNFWASWCVACREEHPSLLAAW
                     DRYRERGVVLIGIDFEDTHEAALAYTDEMGGDWPLVTDPGSRTAIAYGVFGVPETFVI
                     APDGTITAKKLLIHRSTV"
     gene            complement(4461..7028)
                     /locus_tag="VF195_06580"
     CDS             complement(4461..7028)
                     /locus_tag="VF195_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009299298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6580518.1"
                     /translation="METRKTTSTVLHVGGMYRATEKSVVEHVLASRPGVMSVEANPVA
                     QTANVTFDPAETSVADLRRWVEDCGFHCAGQSVPEHICDPLMEPDPPHAMHAGHAPVA
                     VAEPVAEPAEHAGHAGHGAATAPPAEEHAGHAGHGAATAPPAEERAGHEMSAGAMPSP
                     HEMMGHGGNAGMSMDAMVRDMRNRFLVAALLSIPILLWSPIGRDVLGFTAAAPFGLRD
                     DVWLLLLSLPVIFYSAWIFFDGAVRALRARTLDMMVLVAVAIGAGWLYSLVITLTGGG
                     ETFYEAATVLAAFVLMGHWFEMRARGGANDAIRTLLDLAPPKATVIRGGETVEIPTAE
                     VAVGDLMLVRPGDKVGVDGVVEEGQSEVDESVVTGESLPVSKAPGSQVIGATINKNGT
                     LRVRATKVGADTALAQIVKLVQEAQNSKAPGQRLADRAAFWLVFVALIGGAATLGIWL
                     GVGESFSTAMLYAITVVVITCPDALGLATPTAIMVGTGLGAKRGILFKNAVALETSAR
                     IQAVVMDKTGTLTKGEPEVTDVIVDGMPEMEVLRLVAAVERESEHPLAEAVVKHAEAL
                     GVQSVRADKFENVPGHGAMAFVEDRRVVVGNVRLIGREGIDLGPLAARREEMTSSGRT
                     VVCAAVDGRPVALIGIADAPRPTSAAAVSALRDEGIEVVMLTGDNAATAKRIAEQLGV
                     QTVIAEVLPGDKAGKIEELQRNGKKVAMVGDGVNDAPALAQADLGIAIGAGTDVAIET
                     ADVVLMRSDPLDVPTALAIGRGTLRKMRQNLGWAIGYNSVALPIAAGVFEPAFGLVLR
                     PEIAALSMSGSSLLVAVNALLLKGLRLPDSVAPGSKAAGASEAEQTAPTRSDSIPSG"
     gene            7837..8322
                     /locus_tag="VF195_06585"
     CDS             7837..8322
                     /locus_tag="VF195_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580519.1"
                     /translation="MSFDRWEVIRKRRSLRVLAPFLLLLAACASDEGLSSGGAATSEC
                     WFESLPRMVATSDVVVLGTVVEIQKDVMLGTPPEEIYLLHADLDLEETLYGSDVPSPL
                     TVQTDQDVPTEPEWRQVGNTVLAFMKFSTDPNFPGVYYPGEPPVRLSRGGNRPTGDSG
                     G"
     gene            8661..8834
                     /locus_tag="VF195_06590"
     CDS             8661..8834
                     /locus_tag="VF195_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580520.1"
                     /translation="MVRSVGGAMVLLVILVLNVYKPPGLTRHGWRKQQEERRKQQDPE
                     FRRVSAAGATHPE"
     gene            complement(8803..9381)
                     /locus_tag="VF195_06595"
     CDS             complement(8803..9381)
                     /locus_tag="VF195_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012286338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6580521.1"
                     /translation="MPKLWTETIKAHRREVHDAILDTTAALVAEHGLLAVTMSQIAQE
                     TGIGRATLYKYFPDVEAILGAWHGRQITGHLAYLAEVRDRGGGPRERLEAVLEAFAII
                     SLGSRDHHDADLAAFLHRDERVARAQHQVHEMIRDLLVQAAKSGDVRDDVSPDELAGY
                     CLNALSGASRQRSKAAVRRLVTVTLDGLRPRR"
     gene            9482..9658
                     /locus_tag="VF195_06600"
     CDS             9482..9658
                     /locus_tag="VF195_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580522.1"
                     /translation="MTEPLRHPGTDGNGGPGTEGSPGGASRWKVVLGVTIAVALLVLI
                     ILLHTTGTIGPGAH"
     gene            9672..10436
                     /locus_tag="VF195_06605"
     CDS             9672..10436
                     /locus_tag="VF195_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6580523.1"
                     /translation="MSDRFDERGIPVPPRPGARDPVVVVRSLTKRYGDLVAVDDLTFS
                     LRAGTVTGFLGPNGAGKTTTLRLLLGLAEPTSGEALVFGRRYHELDDPARRVGAVLES
                     SDFHPGRSGRDHLRALSLAAAIPHSRVEEVLALVELEGAAGRRVKTYSLGMRQRLGLA
                     AALLGEPELLVLDEPANGLDPAGVHWLRRFLRWFAEQGRSVLVSSHMLAEAAQTVDEI
                     VIIDRGHRVASGRLSELTERGGTLEDIFLELTGGTA"
     gene            10433..11182
                     /locus_tag="VF195_06610"
     CDS             10433..11182
                     /locus_tag="VF195_06610"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580524.1"
                     /translation="MKALLRAELLKQRSTRTGLGLFAAMLGLVLLAVLLHGFGLAAED
                     LGGTNLQLMVLGRGEFLATLFAALLGAMSITAEFRHGTIRATFLITPRRGRVVAAKAF
                     VSTVFGAGFGLVAGAVAAAAGTSALRARGIEVQLDRGDYVLLIVGAAGAAALWAAIGV
                     GVGALARNQVPTLIGICAWLLFVEGLLAGDVADNLGDIGRFLPGAAASAISGQDPGTL
                     LAPAVGLVLLGLYAVAVALVGALGLSRRDVS"
     gene            11229..11516
                     /locus_tag="VF195_06615"
     CDS             11229..11516
                     /locus_tag="VF195_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580525.1"
                     /translation="MSHAKDATGEDRSPKIRVTVVIANRYAFDTEMATGKQIKEKANI
                     PVGFALYRRTRGGNEPIVDYDTVELHDGDAFFARSVGSPGALSDDQQEVSR"
     gene            11593..12078
                     /locus_tag="VF195_06620"
     CDS             11593..12078
                     /locus_tag="VF195_06620"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6580526.1"
                     /translation="MLHHLVLGSRRRRALTRLANFSGVRSGDRVLVVGCGTGTFTREM
                     AEAAAPGGTALGVDASREAIAHARRLTHHPHCTFSERIAEALDATDGSYDVVVSSLMI
                     HHLREEKRPQAIREMFRVLRPGGRVLIAHFRPPTRRIGRHLIGLVTSPAMQNTPSICS
                     T"
     gene            13147..13419
                     /locus_tag="VF195_06625"
     CDS             13147..13419
                     /locus_tag="VF195_06625"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEX6580527.1"
                     /translation="MRLHVHVVGRSCAGLGYRWGSCGRGNKLYFHWMSVLLPPRIVEY
                     VVVHELVHLAEPRHSPDSWLRVERAMPDFASRKKWLAVNGHMVGGI"
     gene            complement(13444..13824)
                     /locus_tag="VF195_06630"
     CDS             complement(13444..13824)
                     /locus_tag="VF195_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580528.1"
                     /translation="MRFDVRRATESNDDFRKRINQLALAEEEKRPPSHGSDSSEWYLG
                     PDYRVSGSLHTDIWRGTAADLAQRGAIAVYPVTGWWKERPSHDHSERGARYALVVSIE
                     TPDQDVDIWTPVAQQIGIPIQITT"
     gene            complement(13936..14009)
                     /locus_tag="VF195_06635"
     tRNA            complement(13936..14009)
                     /locus_tag="VF195_06635"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13973..13975),aa:Met,seq:cat)
     gene            complement(14053..14448)
                     /locus_tag="VF195_06640"
     CDS             complement(14053..14448)
                     /locus_tag="VF195_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6580529.1"
                     /translation="MRRRLLVLSLVLLAAACAPASASPTVRTVRVGIHYSAFSLAVID
                     VDPGDTVRFVVSNADPIDHEFIIGDEAVQIAHERGTEAFHPPRSGEITIPAGETVETT
                     YTFGARDLIFGCHLPGHYTYGMRGVISVG"
     gene            complement(14457..>14961)
                     /locus_tag="VF195_06645"
     CDS             complement(14457..>14961)
                     /locus_tag="VF195_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580530.1"
                     /translation="IPTTNPWVPLRILGLGAAADAPVEADVYLLTDLEPTTLPAPLTP
                     EVASRLPQGVRPGLVLERSEEASRSLLADLRRDKGMDWLPSSGMWLSFLRLDARAGDL
                     DYDLAIDASGAGQPSPVAAGLSPEAAADPAGVRSFGPALWTAIALLVLLGVVGTLERR
                     RGGRPAV"
BASE COUNT         2481 a         5081 c         5029 g         2370 t
ORIGIN      
        1 gccgttccac accggcggct gttgcgcaga cgggtgggtg ggcggccatg gatgccatgc
       61 acgactcccc cgcgatgcaa cgggcacacg cccagatgcc cgaggagcta cgggcccagt
      121 gcgaggccat gcacgagcag atggagcaga tgatgggagc cggcggcatg atgagtggcg
      181 acatgggttc tcaccactca ggcatggccg ccgacggcat gatgggcacg ggtcgggacg
      241 tctcggcggg atgacgggcg ccgggtcggg cagcgtgatg gggagctgag gaggacgtga
      301 tgccctacgg ctggcacgac ggtggatggg gggttctttg gatgatcctc tcgtgggccc
      361 tcatcatcgc gatcgtggtc cttgtggtgc gcgccttctc atccggctcg tcggatcggt
      421 tggaccggac caacgatcca cgagcgatcc tcgacgagcg gttcgcgcgc ggcgagatct
      481 ccgaggacga gtaccggggg cgcacgagcg tgctcgagga gacacgacgc tgaggcacgg
      541 tcgcggcgag gaggaagaac gtggggccgg gcgctgcccg gccccacgac tgaggagggg
      601 ggtgctacaa aggaaggtta ctacacggcg tcgtaaaagg cctcaaccca gccgcgtcca
      661 ctcccgagtc ggtcgacgcc tacgacacgg gaccgtgcgg agggtgacac gtgtcgtgca
      721 gagcgagggc caaccgctca cgtgtcagcg cctgctggcc cgccgaccag gccgctccct
      781 gcgcgagcac catcgcgatc gtcgccagga cgagcacgac caccaggctg cgcctcatcc
      841 tcggccgcct ccgagccgtg agctgccgga tcctgtagcc gatctcaccg tcggtgatgc
      901 tggcgacggc cgcagactgc tcgaacgtga ggcgcgccgt cgtggccaac gcccgggcga
      961 cgaacagcgg gtcccccacc gacgtcgtcg cctgctcatc ggcccaggcc tccagcgcgg
     1021 tcaccagcag ctccaccgac accaggacgc cgggaacgaa gccgaaggct cgagcgacga
     1081 tcctcgccgc tgacagggcg gccatgtggc ggcgcgatgc atgtgctcgc tcgtgggcaa
     1141 taacggctga tcgctccttt ggagcaagcg cgcgcatcag cccatcggag acgaccgtca
     1201 ccgggcgaac gagcccggta gtgaacgcga cggatccctc gaacgggagc agccgaaccg
     1261 acgaggggac gatgcccagg tgttcacgta acgcacgctc ctcaccgggg aaccggctgg
     1321 tgggcggccg ggctcgtttc gcgtcccgcg caacgaggac ggcggcggcg atgacacgga
     1381 cggcggctgc caggaacaag acggcggcca ggatcgacgg ccagtgcttc aggggatgcg
     1441 agagcagtcg gccggccgca ccgatgcaga tctcaacggc tcggggaagg tcaaccaggg
     1501 gcaaagggcc cggcgagacc gtcgcggcga ggacggcgaa caaccccgcg ccgatcccga
     1561 ggagcgacgt cacgccgaac cacgcgagga ccatcggaga cccgcgggca agcaggctcg
     1621 ttcgcaacag atgcgggagc agggcgaagg atacgaagac acccgcggcg agggcgagcc
     1681 tcatgagcga cggccgctcc tcccctcacg ctgagcctgt ttcacgagac gctcgagctc
     1741 cttgaggtca tcttccttca cgctcctgac gaacccgagg aggacgctcc tgcggtcctt
     1801 cgatgcggcg agcaccgaac gaacgagggc ggccgaatag tcgtctcgag acacccgagt
     1861 cttgtacgag taggcgcgcc ccacctttcg ccgcacgagg aaccccttcg tcgagagccg
     1921 ttccatcacg gtcatcacgg tcgtgtaggc gaggccgttc cgtaccccga ccgaatcgac
     1981 cacctcgcgg accgtcacgg gatcgccccg cttccaggcg tgatccatga tctccgcctc
     2041 gagcggtccg agctctccga tcagtttggc cacgccgacc ctcccggtga ggcgagcatc
     2101 ctagccgatg cccgttcgat cgctccttgg ggctcgggta gcgagctggt tggtccgtca
     2161 gcggccttcg tcccaaccga cgaagttcct ccaacaccag ctcgaggacc acgggatctt
     2221 cctgaagctc cgccagccac tccgcgaggg ttccggaccc tcatccgaat gcagctcgcg
     2281 gagcaccgcg gctgtctcct cgcgggggac tcgagcacga ggagcaggag ggcatggatt
     2341 tctcgcggaa gggtcgcgag cgcaccccgc agtcgcgact cgaagtcggg gatctcgccg
     2401 cccacctgct gccctctccg cccgcctatc tactatcctg cgtcgtagcg tagcaaggcc
     2461 cgagcgagcg aggaggggga tggcgcgcgc gaaccgacgc aggacgggcc cttcgcgacc
     2521 ccggaacaag cgccgggata tccaggcccg cgacacgcag gcctcccagc agcgagcgat
     2581 gcagcgcaag cagatcacgg tccgcgcgta tcggttccgc cgagcgctcg gctggggact
     2641 cgtggggctc ggcatcgtcg tcggggtgag ccactggctc tcgcacatcg ggttctgggg
     2701 gttcgcctct cagggcgtga tggacctcgt ggccggctac ccgatggcgg cggcccttgt
     2761 gatagcggga tcgatcgtgc tgggccggtt gtcgaagtag cgatcgatca gcctctccgc
     2821 ggcgccgcgc ggtcgcagcc gtcgtcgggg tcgaacccgg tctcagatcg gcggccaccc
     2881 caggctcgaa aagagacgga cgagcggaga gaacatccgg atccaggtcc cggtgagcat
     2941 cagcacgccg atcgcgatga gcaccaggcc ccccctcgtt cgaccgcccg gccgtgccgc
     3001 cagagccact cgaacgcccg gagcgatcgc gagtacccga gggcaaggag aacgaagggg
     3061 atccccatcc cgatcgagaa cgtgaacagg agcagcgcgc ctctgaatgc cgactgagcc
     3121 gacgctgtcg ccgtgaggat cccggccagc accggaccga tgcacggcgt ccacctgatc
     3181 gcgaaggcca tcccgagcgg gatcgcgccc gccggccccg tcctgatccg gcggaggtcg
     3241 aggcggcgct cccgatagag gaacgagaat cggaggacgc cgatcgcgac cagccccatc
     3301 gcgatcacga agatcgcggc cacacgagtg aggaggatcc ggttgcggag gaggaaggtg
     3361 cccgcggccg aagcgctcgc gccaaggagc gtgaacacgc tggcgaaacc ggagacgaag
     3421 agcagcgccg atcccacgac acggctccgc acagccaccg agccacccgc gtcaccggcg
     3481 acaccactca cgtaggagac gtagcccggc atcaagggaa gggagcaggg cgaggtgaac
     3541 gccagcatcc ctgccacaag tgcgagcgca agtgcggtca cggcgcagga ctcttcgcga
     3601 aagcaggcgg tcacagagcc cctgggcgag caggatcagc ctcatcgcga cggcgaaccg
     3661 ggctcggtcg cgcgctcggc agcggtcacg aacgtggaac ggcacaggat cgacttccat
     3721 gtcgcagact ggatcgatcc tcatctcagg ctctcgcgac cacctactat cctctgtcgt
     3781 acgatagccg ggtgccatcg cctgagtcac ccttgctgag ggaaggtcgt ggaagcagcg
     3841 ttggccgaag cgccgatcgt gatcgggccg acgaaggcgc gccgagcgaa cgtgcagtgg
     3901 tcgaggagca actcgatgag ggcgacccaa ccccgccgca tcgacggggt cgcctgctgc
     3961 tcgccgtact ggtgccggtg atggcgttcg cgctcctgct cgccaccgga cttggcgggg
     4021 acccgcgcgc gctgccgagc gaactcgtac gaaggccagc gccgtcgttc gtgctgcccc
     4081 ggctcagcgg cggcgggagc gtgcacctga ggcagcttcg agggcaggtc gtggtcgtga
     4141 acttctgggc ctcgtggtgc gtcgcgtgtc gggaggagca cccgagcctg ctcgcggcct
     4201 gggaccgtta ccgagagcga ggggtcgtcc tcatcgggat cgacttcgag gacacgcacg
     4261 aagcggcgct cgcctacacc gacgagatgg gtggggactg gccgctcgtc acagaccccg
     4321 gctctcggac ggcgatcgcc tacggtgtgt tcggcgtccc cgagacgttc gtgatcgcac
     4381 ctgacggcac catcacagcg aagaagctgc tgatccatcg atcgacggtc tgatagagat
     4441 ccttccgcct tgcctgtccc tcatcccgat gggatcgagt ctgatcgggt cggagccgtc
     4501 tgctcggcct cgctggctcc tgccgccttc gaccccggag ccactgagtc gggcaggcgg
     4561 agtcccttca gcaagagcgc gttcaccgcc acgaggaggc tcgagcccga catcgacagg
     4621 gccgcgatct ccggccgaag gaccagcccg aacgccggct cgaacacccc cgccgcgatc
     4681 gggagcgcca ccgagttgta gccgatggcc cagccgaggt tctgccgcat cttgcgcagg
     4741 gtcccccgac cgatcgccag ggcggtgggc acgtcgagcg ggtccgagcg catcagcacc
     4801 acgtcggccg tttcgatggc gacgtccgtc cccgccccga tggcgatgcc gaggtcggcc
     4861 tgggcgagag cgggggcgtc gttcacgccg tcccccacca tggcgacctt cttcccgttg
     4921 cgctggagct cctcgatctt gccggccttg tcccccggga gcacctcggc gatcaccgtc
     4981 tgcaccccga gctgctccgc gatccgcttc gccgtggccg cgttgtcgcc cgtgagcatc
     5041 acgacctcga tgccctcatc gcgaagggct gagaccgcgg ccgcggacgt cgggcgaggg
     5101 gcgtccgcga tgccgatgag cgccaccggc cggccatcca cggcggcgca cacgacggtc
     5161 cgcccgctcg aggtcatctc ctcccgacgc gccgcgagcg gaccgaggtc gatgccctcc
     5221 cgaccgatca agcggacgtt tcccaccacc acccttcggt cctcgacgaa ggccatcgcg
     5281 ccgtgtcctg ggacgttctc gaacttgtcc gcgcgaacgc tctggacgcc gagtgcctcg
     5341 gcatgcttga ccaccgcctc ggccagagga tgctcggact cccgctcgac cgcggccacc
     5401 agccggagca cctccatctc tggcatgccg tccacgatca cgtcggtgac ctcgggttcc
     5461 cccttggtca gcgtgccggt cttgtccatc accacggcct ggatccgagc cgaggtctcc
     5521 agagcgacgg cgttcttgaa caggatcccc cgtttcgctc cgaggccggt tcccaccatg
     5581 atcgccgtag gcgtggccag gccgagggcg tcggggcagg tgatcacgac gacggtgatg
     5641 gcatacagca tggccgtgct gaatgactcc cccaccccca gccagatccc cagggtcgcc
     5701 gcgccaccga tcagggcgac gaacacgagc cagaaggcgg cgcggtccgc cagccgctgc
     5761 ccgggcgcct tcgagttctg ggcctcctgc accagcttca cgatctgggc caaggccgtg
     5821 tcggcgccga ccttggtggc ccggacccgg agcgtcccgt tcttgttgat ggtcgccccg
     5881 atcacctgcg agccgggtgc cttcgacacg ggaaggctct cgccggtgac caccgattcg
     5941 tcgacctcgc tctggccttc ctccaccacc ccgtcgacgc cgaccttgtc gccgggacgc
     6001 accagcatca gatcgccgac cgcgacctcg gccgttggga tctccacggt ctcgccgccc
     6061 cggatgacgg tcgccttcgg tggcgccagg tcgagcaggg tcctgatggc atcgttcgcc
     6121 ccgccccgtg cccgcatctc gaaccagtgg cccatgagga cgaaggccgc gagcaccgtg
     6181 gccgcctcgt agaaggtctc cccgccgccg gtcagcgtga tcaccagcga gtacagccaa
     6241 cccgcgccga tcgccaccgc gaccaggacc atcatgtcga gcgtgcgggc tcgaagggcc
     6301 cggacggcgc cgtcgaagaa gatccatgcg gagtagaaga tcaccggaag actgagcagc
     6361 aggagccaga cgtcgtcacg gaggccgaag ggcgcagcag ccgtgaagcc gaggacgtcg
     6421 cgtccgatcg gagaccacag caggatcggg atcgacagca gcgcggccac gaggaaccgg
     6481 ttgcgcatgt ctcggaccat ggcgtccatc gacatgccgg cgttgccgcc gtggcccatc
     6541 atctcgtgcg gtgagggcat cgcccccgca ctcatctcgt gcccggcgcg ctcttcggcg
     6601 ggcggggccg tcgcggctcc gtgtcccgcg tgcccggcgt gctcttcggc gggcggggcc
     6661 gttgcggctc cgtgtcccgc gtgcccggcg tgctctgcag gctcggcgac aggctcggcc
     6721 accgcgaccg gcgcgtgacc ggcatgcatc gcatgtgggg gatcgggctc catcaagggg
     6781 tcgcagatgt gctccggaac cgactggccc gcgcagtgga agccgcagtc ctccacccat
     6841 cgacgaaggt cagccaccga agtctccgcc gggtcgaagg taacgttcgc cgtctgcgcg
     6901 acgggattcg cctcgacgct catcactccg ggacgacttg cgaggacgtg ctccaccacg
     6961 gatttctcgg tggcgcggta catcccgccg acgtgcagta ccgtcgaggt agtcttccgc
     7021 gtctccatcg gtccgtgacc ctctgcctgg ctcgtgttgt gggcgcccgc tcgagcgaat
     7081 ccgttccggc gttcctcttc catgtggctc acgactccaa gccaaccaca gatgccccaa
     7141 ccgagacggg cagtagtcgg catggcgctg caagaagccc gccggcgagc cggcgaactc
     7201 ctcccacggc ttcacccgtg agcaggccta tctactatct tccgtcgtag gatactcgga
     7261 cctcgatctg gagaggaaca tccgttggga ggctccaggg tcgctggacc ggtccgtcgc
     7321 gcgtcgaaca agctcgcgga cggcccggtc ccccggcgca ggcagcacag cggtcagcgc
     7381 gagcgagatg agcctgctcg cgcctatcgc cttcccaagg cgcatggctg gtcgcgcatc
     7441 ggcccgagac catcgtgggt atgagcacca ctcacgacat gcagctccgg gcttcgcatc
     7501 gccacgggtc acggacctcg ttgcgggatg ccccacggcg tttctcctgg ccatccatga
     7561 caccatcgtc ctctcccgat gagccgtgtc cggctcatga ctaggcgtga cgctctggtg
     7621 ctgcttctct cgttcatcgt cggagccgcg ctgggtagcc tcaccgtaga gatcctccgc
     7681 tgacagcatc gagggagggc gcgagaccga gcgaaggcaa caagaggcgg tctcccctcg
     7741 gtctgtgctt cgggtgcagg agatcatcct catctcacct gattgagcac ggcaccggta
     7801 tcctcgacgc gcaatgcgga tgacgacgat caggttgtgt cgtttgatcg ttgggaggtc
     7861 atcaggaagc gacgaagctt gagggttctg gctccgttcc ttctgctgct cgccgcatgc
     7921 gcgtccgatg agggactgtc gtccggaggg gcggcgacga gcgagtgctg gttcgaatcc
     7981 ctgccgcgga tggttgccac atccgacgtt gtggtgctgg gtaccgtggt ggagatccag
     8041 aaggacgtca tgctgggaac ccctcctgag gagatctacc tgctccacgc cgatctcgat
     8101 ctcgaggaga ccctctacgg gtctgacgtg ccgtcaccgc ttacggttca aaccgatcag
     8161 gacgtcccga cagagccgga atggcgacag gtcggtaaca cggtgcttgc gttcatgaag
     8221 ttcagcacgg acccgaactt tcccggtgtc tactaccccg gtgaaccccc agtccgtcta
     8281 tctcgtggcg ggaaccgacc tacaggagac agtggaggct gatcccttct ccgaaggcgt
     8341 agcgaagatg actctcgatc agattcgcag caagaacgag caagcaaagc ggtccatcgc
     8401 cgccggagag gtcacagcgc agaagcctgt cggcggctga gacagcaatc ggctggccga
     8461 gatccaagga gtgtcgtttc cgcttggacc gctggctccc atgggggcgc gtgatcgggg
     8521 tgactgtgcg tcggccggat cgtagctgac cgtcattgcg acggccgact tgctatcgga
     8581 aacgcagacg atcagccacc tcgcagacac ggcggattcg acaacttcca gcgacgatct
     8641 ccgcgtcctt ggaagcacaa atggtccgct ccgtaggcgg cgcgatggtg ttgctggtga
     8701 tcttggtgct gaacgtgtac aagccgccgg gcctgacgcg gcatgggtgg cggaagcagc
     8761 aggaggagcg gaggaagcag caggacccgg agttcaggcg cgtcagcgcc gcgggcgcaa
     8821 cccatccaga gtgaccgtta ccagccgacg aacggcggcc ttcgagcgct gtctgctggc
     8881 tcccgagagg gcgttcaggc agtagccggc gagctcgtca ggcgacacat cgtcccggac
     8941 atcgccggac tttgcggcct ggaccagcaa gtcccggatc atctcgtgga cctggtgttg
     9001 cgcccgagcc acccgctcgt cccggtgaag gaacgccgcg aggtcggcgt catggtgatc
     9061 gcgggatcca agagagatga tggcgaaggc ctcgagcacc gcttcgagtc gctcgcgagg
     9121 gccgcctccc cggtcccgga cttcggcgag atacgcgagg tggccggtga tctgacgtcc
     9181 gtgccacgcg cccaggatcg cttcgacgtc ggggaagtac ttgtacagcg tcgcacgtcc
     9241 gatgccggtc tcctgggcga tctgcgacat cgtcaccgcc agcagcccgt gctccgccac
     9301 gagcgccgcc gtggtgtcca ggatcgcatc gtgcacctcg cggcggtgcg ccttgatcgt
     9361 ttcggtccac agcttcggca cgggcgaagt atacgttacg ccgatactca gtcgtcttga
     9421 tttgacggag acagtttgtc tcgataacct tggctgctat caacacacag agggaaccgc
     9481 gatgaccgag ccgctccgcc accccggaac cgacgggaat ggcggcccgg gcaccgaagg
     9541 atcccctggc ggcgcatcac gctggaaggt cgtactcggg gtcacgatcg cggtcgcgct
     9601 gctcgtgctg atcatcctcc tgcacaccac cggcaccatc ggccccggcg cccactaagc
     9661 gggagcggaa cgtgtccgat cgcttcgacg aacgcggcat ccccgtccct ccacgcccag
     9721 gggctcggga cccggtggtc gtggtccgct cgctgaccaa gcgctacggc gacctcgtcg
     9781 ccgtcgacga cctcaccttc tcgctccgtg ctggcaccgt caccggcttc ctcggcccga
     9841 acggcgccgg gaagacgacc accctgcggc tgctcctcgg gctcgccgag ccgacctcgg
     9901 gcgaagcgct cgtcttcggc cggcgctacc acgagctcga cgacccggcg cgacgggtcg
     9961 gcgcggtcct ggagtcgagc gacttccacc ccggacgctc cgggcgcgac cacctgcgcg
    10021 cgctctcgct cgcggcggcg atcccgcaca gccgcgtcga ggaggtgctg gcgctggtcg
    10081 agctcgaggg tgccgccggg cggcgggtga agacgtactc gctggggatg cggcagcggc
    10141 tcgggctggc ggcggcgctg ctcggcgagc ccgagctgct cgtcctggac gagccggcca
    10201 acgggctcga tcccgcgggt gtgcactggc tgaggcgctt cctgcgctgg ttcgccgagc
    10261 aaggccgctc ggtgctcgtc tcgagccaca tgctcgccga ggccgcacaa acggtcgatg
    10321 agatcgtgat catcgaccgc ggccaccggg tcgcgagtgg gcggctctcc gagctgaccg
    10381 agcgcggagg gaccctcgaa gacatctttc tagaactgac cggagggaca gcgtgaaggc
    10441 cctgcttcgc gccgagctgc taaagcagcg atcgacccgg accggcctcg gcctgttcgc
    10501 cgccatgctc ggcctcgtcc tgctcgccgt gctcctgcat ggcttcggcc tcgcggccga
    10561 ggacctcggc ggtaccaact tgcagctgat ggtgctcgga cgtggggagt tcctggccac
    10621 cttgttcgcc gccctcctcg gcgcgatgtc gatcaccgcc gagttccgcc acgggacgat
    10681 ccgtgcgacg ttcctcatca ccccgcgccg cggccgcgtc gtcgcggcga aggccttcgt
    10741 gagcaccgtg ttcggggcgg gcttcggact cgtggccgga gccgtcgcgg ccgccgcggg
    10801 cacctcggcg ctccgggcac gagggatcga agtgcaactc gaccggggcg actacgtgct
    10861 gctcatcgtg ggagccgcgg gtgcggccgc gctgtgggcg gcgatcggcg tgggcgtggg
    10921 cgcccttgct cgcaaccagg tcccgacgtt gatcggcatc tgtgcgtggc tgctcttcgt
    10981 cgagggcctg ctggccggcg acgtcgcgga caacctcggc gacatcggcc ggttcctgcc
    11041 cggcgcggcc gcatcggcga tcagcggaca ggatccgggg acgctgctcg cgccggcggt
    11101 cggcctcgtg ctgctgggcc tctacgcggt cgccgtggct ctggtcggcg cgctcggact
    11161 ctcgcgccgt gatgtcagct aggtcagcgt cgccgtcccc gcgtggccgc tcggaaaggg
    11221 gcgcagacat gtcccacgcg aaggacgcca cgggcgagga ccggtcgccg aagatccgcg
    11281 tcaccgtggt catcgcgaac cggtacgcgt tcgataccga gatggcaacg ggaaagcaga
    11341 tcaaggagaa ggccaacatc ccggtgggct tcgccctgta ccgccgcacg cgcggcggga
    11401 acgagccgat cgtcgactac gacacggtcg agctgcacga cggcgatgcc ttcttcgccc
    11461 ggtctgtggg gtctcccggc gcactgagcg atgaccaaca agaggtctcg agatgaacaa
    11521 gctcacgtcg ggcacggcac ggcgggtgct ggcacactcg gcatgcatct gggacacgga
    11581 cgaagctacg acgtgctgca ccacctcgtc ctcggcagcc gccgacggcg cgccctcacc
    11641 cgcctggcca acttcagcgg cgtccgctca ggcgaccggg ttctcgtcgt cggatgcggc
    11701 accggcacct tcacccgcga gatggccgaa gcagccgctc ctggcggcac cgcgctggga
    11761 gtggatgcct cccgagaggc catcgcacac gcacgccgtc tcacccacca cccccactgc
    11821 accttctcgg agaggatcgc cgaagccctc gacgcgacgg acggctcgta cgacgtcgtg
    11881 gtgagcagct tgatgatcca tcatctacgc gaggaaaagc gccctcaggc gatccgcgag
    11941 atgttccgcg tgctccgccc cgggggccgg gtgctcatcg cccacttccg accgcccacc
    12001 aggcgaatcg gccgccacct gatcggtctc gtcaccagcc cggccatgca gaacaccccg
    12061 tccatctgct cgacctgatg gttcgtgagg ccggcttcga gcaggcactt tgcggcgatg
    12121 tccacccgtg gatccggtac gtgcaggctg cgaagccaac caacgcccca tcggcgcaga
    12181 aggcgtcgtc tacgagggca cacttcggcg tcgacggtgg tcgctcgagc gccgggtgct
    12241 gcgccgccga atcgacggag attggctctc cggcggctcc gacgtcgatg acggacgtcg
    12301 gctcgggccg ccgagcgcag tgagggcgag ggagcccggt gtcaggccgt gacgtgctcg
    12361 acgacggcat gctcgcgatc gccaccgtcc tcgcggccat tggagtcgga ggtctgatcc
    12421 ttgatgaggt aagggcggta cgagccgggg gaagaggacg attcagggcg gatctcggat
    12481 ggacgctcct ttgggtgatc ggcttgagcg cgcttttcgc ggccgcttgg gaatcacgac
    12541 gctgaggaac agcggttcat ctggcaagtg acaggatttc ccggtgtgga ggctagctcc
    12601 agcaaacccc tcggccgact ccctcgccag tcagctcgcc tagatcggtg agaggttcta
    12661 cgactgggcg catccgaacg gctccatcga gcccgaccag gccaacgtga tcagctaagg
    12721 aactgacgtg ccgcttgcgt ttcgcttcga tcattccggt caagcaatca gcacaagggg
    12781 cctcgtctcc gagggaaagc gccgtagcgc cgtacggcgg gacatcgaaa gagcctatcc
    12841 agctcgacga gacaatgtcg ttgccgcgga tcacgaccat cgaccttctc gatccgcgga
    12901 caacgttcct tggcgtagac ctaccagttg gcgcttcggg tcggtcgaat gaccttctcc
    12961 ttgtagaggc ggcggcgagc cctccagaat ctccacctat ctcgcagggt cttcggatcg
    13021 ccgacttcgc gccttaggtc ttccaccgcg cgctgatact cctgctccag cgcgtctcgc
    13081 gggttcagga acccgctgga ttcgcgactc atctatctag cgtagcgcca ccttctcgat
    13141 ccgcggattc ggctccacgt acacgttgtt ggacgttcct gtgcgggact tgggtatcgc
    13201 tggggttcct gtggcagggg caacaagctc tacttccatt ggatgagtgt tctgctgccg
    13261 ccgcggatcg tcgagtacgt cgttgtgcac gagcttgtgc atctagcgga gccgcggcat
    13321 tcgccggact cctggctccg tgtggagcgg gcgatgccgg acttcgcgag tcgaaaaaag
    13381 tggttggccg tgaacgggca catggttggc ggcatctgat gatgcgtgct gcgactcggt
    13441 cgatcaggtc gtaatctgaa tcggaatgcc aatctgctgc gccactggcg tccagatgtc
    13501 gacgtcctga tcgggcgttt cgattgagac gacaagagcg tagcgtgcac ctcgctcgct
    13561 gtgatcgtgg gacggccgtt ctttccacca ccccgtcact gggtagacgg cgatcgcgcc
    13621 tcgctgggca aggtccgcgg ccgtcccacg ccagatgtcg gtgtgaagag agccggacac
    13681 ccggtagtcg ggtcccaagt accactcgga ggagtcgctg ccatggcttg gtggccgctt
    13741 ctcttcttcg gctagggcga gctgattgat tcgtttccga aagtcgtcgt tcgactcggt
    13801 cgcgcgacga acgtcgaacc gcaagccatg cgacggatag atgtatcggc gaacccaacc
    13861 ccatcgggta cggttcctag gcattttccc tggtaaacag actgaagggc caccgccttg
    13921 gtggcccttc tttcgtcgcg gggacgggat ttgaacccgt gacctctggg ttatgagccc
    13981 agcgagctac cggactgctc caccccgcag catgatgata ccgaccaacc cttgggcgtg
    14041 ccaatcgtgc ggtcacccca cggagataac gcctcgcatc ccgtacgtgt agtggcccgg
    14101 caggtgacaa ccgaagatca ggtcccgcgc gccgaaggtg taggtcgtct cgaccgtctc
    14161 gccggccggg atcgtgatct ctcccgagcg cggcgggtgg aacgcctcgg tcccgcgctc
    14221 gtgcgcgatc tgtaccgcct cgtccccgat gatgaactcg tggtcgatcg gatccgcgtt
    14281 cgagaccacg aagcgcaccg tgtcgcccgg atccacgtcg atgaccgcga gggagaacgc
    14341 cgagtagtgg atcccgaccc gcacggtcct gaccgtcgga gatgcggacg ccggcgcaca
    14401 ggctgccgcg agcaggacca gcgacagcac gagcagccgg cgcctcatca ctcgcctcag
    14461 accgcgggac gaccgccgcg acgtcgctcg agcgtcccga ccacgccgag gagcaccaag
    14521 agggcgatcg ctgtccacag cgctggaccg aacgaccgta cgccggcagg gtcggcggcc
    14581 gcttcggggc tgaggccggc ggccacgggg gaaggctggc ctgcgccgct cgcgtcgatc
    14641 gcgaggtcat agtcgagatc tccggctcga gcgtcgaggc ggaggaagga gagccacata
    14701 ccggaagagg gcaaccagtc catgcccttg tccctccgaa ggtcggcgag cagcgagcgc
    14761 gaggcctcct cgctgcgttc gagcaccaga cccgggcgca cgccctgcgg gagccgcgac
    14821 gcgacctccg gtgtgagcgg cgcgggaagc gtggtgggct cgaggtcggt caggaggtag
    14881 acgtcggcct cgacgggcgc gtcggcagcc gcaccgagac ccaggatgcg gaggggtacc
    14941 cacgggttcg tggtggggat g
//
LOCUS       DASUYD010000123         3559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_727626, whole genome shotgun sequence.
ACCESSION   DASUYD010000123 DASUYD010000000
VERSION     DASUYD010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_727626"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF195_06650"
     CDS             <1..290
                     /locus_tag="VF195_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580531.1"
                     /translation="PTSDLGEAELHPSQPGEQHAAPGTAAPPPEPPLAVAGWTPKGRS
                     PATDQRRSPGSTHRIEHRVLVGLQQPGPDLGSQLPLGVDRRSFEPDEVLGF"
     gene            complement(1104..3107)
                     /locus_tag="VF195_06655"
     CDS             complement(1104..3107)
                     /locus_tag="VF195_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6580532.1"
                     /translation="MLAHLKSPRPRISGAVRVWCLTGVLVALSLAALLMAADSMAPPL
                     GGIQVPWWTLAAGFAVTEVFVLDVSSSRNERWLTLSEVPLVIGLVYSTPATIVAAQAL
                     GVAAVFVLYRREKPLRCAFNVAQRAAATLVAVFVFVPIVHVAGSTWPIIWLAGFAATL
                     SADIAAGILTNAAIALSEGVRMRFEHILGIGLALTFAKTGLALTVVMVMTEYPAGLVV
                     VAIPVAAAFLAARAHVKVRRERDDMVKLQRATGLAQRSLHPDEMLPVLLEHLRDMFHA
                     DIAELVLPDQAKDHLASRMGPGGAVAILTPIDLDPTQGVWARVVAEGEGVLLARPIRN
                     PLLAEHFSSLGINDAIVAPVSCEDGPSGILTIANRVGDFSTFGAEDLHLLEALAKHIG
                     VTIRNSRLTLRLEDALEREAETNTLKDDFLATISHELRSPLTSMHGYVKTMRAAGDKM
                     PEKEREEFLAAADRAGERLHSLIEDLLFSSRVETPVSGDRLGPVGLAGLVGRVVEDRL
                     EHLETGRIVLRFPSSQPPVWTNEGDVQRIVSNLLDNALKFSPADTPVTVSADTDGAGV
                     RVSFRNLGAEIPESERERIFDRFYQVDHGLTRSNGGIGLGLHICRRTAESLGGRVWLN
                     ESDDSGSVFCLWLPARDVESLDGRDGRRGRAHNRLAHSSASEA"
BASE COUNT          655 a         1166 c         1158 g          580 t
ORIGIN      
        1 caccgacatc cgacctgggc gaggctgagt tgcatccgtc ccagccgggt gagcagcacg
       61 cggcgccggg aacggcggcc ccgccgcctg aacccccgct cgcggttgca ggctggacac
      121 cgaaagggcg ctctccggcc acggatcagc ggcgctcgcc gggctccaca caccgcatcg
      181 agcaccgcgt cctggtaggc ctccagcaac ccggcccaga tctcgggtcg caactcccgc
      241 tcggcgtcga ccgcagatcg ttcgaacccg atgaggtcct gggcttctag ctcgagccgg
      301 agttcccggc taacctcaac ccgaagaagc acggcgtcct cctttgtcga aggggcagga
      361 ctccgtgctt cttcaactcc ggaggaacca aagtcaacga ttcaacccgc tatcgagaag
      421 tcagtcggta ttctcggaac gacactggtc gcagcccacc gttcggttgc ggactgcgtc
      481 cggattgcat ggttccttca ggaggtcgaa gaggatgatt cccacgcggt catcgatccc
      541 ctgagcaagg cgggcggacc tccgtgggtc ttcgttcgaa gacgagggca tacggggatc
      601 tcctggcctg tgcgtgagtg tcccgtgcgg cacgtcccag gccgtaggga tactcaacga
      661 cccgatcggc gagcagcttg acgggcggcc gatcgcgtag gacgtgactc ccttgagtac
      721 cagcggctga agaccgtggt agggattccg gagcacggaa ggtcgatcgc ttcgaccatc
      781 cgttggatcg ggtgtccgtc cacgatcacg agccatcctc cggctcgaag agctgcccgg
      841 ggggagccca tccacgcctc atggtcgcca attcagatca ggggcccatg tcaccgaaga
      901 cgagatcgac ccggcggtgc agctccttgg gaagctcccg agcgtcggcc tgaacgaacg
      961 tggcgtcgag gccaaagtcc ccaggtagtg cggatgacgg gggcaacgtc catcgcggtg
     1021 gcagcgatgc tcctcgccct caccatgatg gagcggatgg cagtgcgtga gctcgaacac
     1081 ctgccgtcgg aacgaggaac cgcctaggcc tcgctggcgc tcgaatgagc caggcggtta
     1141 tgagcacgtc ctcggcgccc gtcgcgaccg tcgagactct cgacgtctct tgcagggagc
     1201 cacaagcaga agacggaccc ggagtcgtct gattcattca gccacacgcg gccacccagg
     1261 gactccgctg tgcgccggca gatatgcagg ccgagcccta ttccgccgtt cgatcgagtg
     1321 agcccatggt ctacctgata gaagcggtcg aagatccgct cgcgctcgga ctcggggatc
     1381 tcggccccga ggttgcggaa cgagacgcgg acacccgctc catcggtgtc cgccgatacg
     1441 gtgaccggcg tgtcggccgg cgagaacttg agcgcgttgt cgaggagatt cgagacgatc
     1501 ctctgtacgt ccccctcgtt cgtccatacc ggtggctgcg aggaagggaa ccgcagaacg
     1561 atccggccgg tttccaagtg ctcgagtcga tcctcgacga cacgcccgac cagtcccgcc
     1621 aatccaaccg gacccaagcg atcgcctgag accggcgttt cgacccggga actgaagagc
     1681 agatcctcga tgagcgagtg cagcctctcg ccggctcggt cggcggcggc gaggaactcc
     1741 tctcgctcct tctcgggcat cttgtccccg gcggcgcgca ttgtcttgac gtacccgtgc
     1801 atactcgtga gcgggctacg cagctcatgc gagatcgtcg caaggaagtc gtctttcagc
     1861 gtgttcgtct cggcctctcg ctcaagtgcg tcctcgagcc gtagcgtcag ccgcgagttc
     1921 cggatcgtga cgccgatgtg tttcgcgagg gcctcaagca ggtggaggtc ctcggcaccg
     1981 aaggtactga agtccccgac tcggtttgcg atcgtcagga tcccggaggg tccgtcctca
     2041 cagctgacgg gggcaacgat ggcatcgttg atccccagcg aactgaagtg ctcagccagc
     2101 agagggttgc ggatcggtcg agcgagcagg acgccttcgc cttctgcgac gacgcgcgcc
     2161 cacacgccct gcgtagggtc gaggtcgatc ggcgtgagga tcgccacggc tcccccaggc
     2221 cccatgcggg atgcgaggtg atccttggcc tggtcgggga ggacgagttc ggcgatatct
     2281 gcgtggaaca tgtcgcgaag gtgctcgagt aggacgggca gcatttcgtc ggggtgcagc
     2341 gatcgctgcg cgagcccggt tgctcgctgc aacttcacca tgtcgtcgcg ctcgcgtcga
     2401 accttgacat gcgcccgagc agccagaaac gcggccgcca ccggaatcgc gaccacgact
     2461 aggccggccg gatattcggt catcaccatc actacggtca acgccagtcc ggtcttcgcg
     2521 aacgtgagcg ccaaaccgat gccgaggatg tgctcgaacc gcatcctcac accctcggaa
     2581 agcgcgatcg cggcattggt caagatgccg gcggcgatgt cggcggacaa ggttgcggcg
     2641 aagccggcga gccatatgat cggccacgtg gaccctgcca cgtggacgat agggacaaac
     2701 acgaagaccg ccaccagcgt cgccgccgct cgttgcgcca cattgaacgc acaacgcagc
     2761 ggcttctcac gtcggtacaa cacgaacaca gcagcgacgc cgagcgcctg cgccgccacg
     2821 atcgttgcgg gcgtcgagta gacgaggccg atgaccagcg ggacctcgga gagggtgagc
     2881 cagcgctcgt tgcgcgaact gctcacatca agcacgaaca cttctgtgac ggcgaacccg
     2941 gcggcgagcg tccaccaggg gacctgaatg cctccgaggg ggggcgccat cgagtccgcg
     3001 gccatcagca gggccgccaa ggagagggct accagcacac ccgtaaggca ccagacacga
     3061 acggcgccgc tgatccgcgg gcgcggcgat ttcagatgtg caagcatcgg tggcctcttc
     3121 ctccaatgag acgattcgac tacaacgaca ttgcgctgaa tcagtcttgg ggctcgtatc
     3181 tcgtcgtgcg cgcaggaaga ctttcggctg gatccgccaa gcgccggcct aggccgtccg
     3241 gcgacggagg cggatgacgg gcggcgactc ctgaggcgac gcgatgcacg caaggcacca
     3301 gccgagatcg tcttgctcgg gcaccggctt cttgacgcag cgccaggaag tcatcctcgg
     3361 atcgacaaga gcgccgcgat tgggacgccc agttagggtc gcctccatcg agtcgtatcg
     3421 gcagaccgac ggggatcgct cgagcggccc aagccggcta catggtcagg gccgggcctt
     3481 cgtgcctcgc cgtcgggagt gacgagcgcg atcttccccg gcgcgtcccc gcccgcgatg
     3541 aggtcgttga agtccgccc
//
LOCUS       DASUYD010000124        26137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_950036, whole genome shotgun sequence.
ACCESSION   DASUYD010000124 DASUYD010000000
VERSION     DASUYD010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 26137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26137
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_950036"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1628
                     /locus_tag="VF195_06660"
     CDS             105..1628
                     /locus_tag="VF195_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HEX6580533.1"
                     /translation="MNDSFVIGCDVGSQGTNAALYGADGELVASAYEAYDLSFPHPGW
                     AEQDPDLWTSAIERACRRLVSECPGGAAAIRGLSFGSQLDGMVVCDASGRRLRAALIW
                     MDRRAEAQAADLAERISGEDFYRAVGANLDSSHAVFKALWVRDEEPELWREAARLMSP
                     GTYVLRHATGVTAVDPSNASSMALLDPRTKTWSPQVLEATEIDRAMLPELGDATRAVG
                     TVTSAFAEASGLAPETVVAVGCGDEMAATLGAGVFEPGEVCDVVGTAEPVCAASAEPR
                     EDPTMLVECHPHADPDAWLLENPGFVSGGNLRWWRDQFAPDERRNEEQGLGDAYDQLS
                     EEASRIAPGSDGVVFLPAMQGAMAPEWNGAARGVFFGLTLAHTRDHMTRAILEGSAFG
                     LRDILEAMKAAGLGVRRLTIVGGGAKGPLWRQIKADVTGLPVRVPESVETTATGAAIL
                     AAVGSGVHATVADAVDSFVSFRPEEHEPDPAARDAYDEAYQRYRDVYFALKPVFGPD"
     gene            complement(1635..2021)
                     /locus_tag="VF195_06665"
     CDS             complement(1635..2021)
                     /locus_tag="VF195_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580534.1"
                     /translation="MKRASLIVLCALLSACFSESGLTDEFRDATNAIDDLVDAIGDEG
                     PVREAVARVRDTVDEADSALEAYRENPNAETRQALEDAERRMSDAREELEGLVDSAPE
                     GIRDAIRTVIDRLANLRDDVARELED"
     gene            complement(2058..3440)
                     /locus_tag="VF195_06670"
     CDS             complement(2058..3440)
                     /locus_tag="VF195_06670"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6580535.1"
                     /translation="MQQQGMIDAKQLEELADAGEIDTVLCMFTDFQGRFVGKRILPHF
                     FLEDVMGDEGLHACLYLIAIDMEMEPLPGFEYASWETGYGDFKMIPDMSTMRLVPWLE
                     KTAMVICDVADEHTSELVEVAPRTILRRQAERAKAAGYTFKTGSELEFYLFKESYEEA
                     AAKRYQGIQPSSPYIMDYHMLATTKDEWLIRQIRNGMLGAGIPIEFSKGEFGRGQHEI
                     NITYADALTTADYHALYKHGTKEIAAMNGAAITFMAKWTMDEAGSSCHMHSSVWNEDG
                     SESLMWDDDDPNHMSPTFKHMLGGLMSTSREMAWMYAPFVNSYKRYQLGSWAPTAILW
                     SHDNRTCGFRVVGEHQGNRVESRLPGGDVNPYLGYAATIAAGLHGMQNQIECPPLFEG
                     NAYESKDAERVPSSLHEAIAEMERSEVMREAFGDFAFGHLVNMARQEQIIFDNQTVTD
                     WELIRYFERG"
     gene            complement(3512..5176)
                     /locus_tag="VF195_06675"
     CDS             complement(3512..5176)
                     /locus_tag="VF195_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6580536.1"
                     /translation="MSERKLGSVTYREVGEDYFDKRGLRRHAGVTSLWALGVGAVISG
                     EYYGWNFGLGTGGFGGLLVAGVIMAIMYYGLVYSIAEMSPALPHTGGAYSFARSAMGP
                     WGGFLTGLAENMEYVITPAVVVGAMGLLMQQIVVGLFEVGGTFAADGSITDIAWWNSL
                     PFWWAVFYVIFVGINIIGIEATMKFTVGITVLSLVVLAFFFIAALVSGKFDPSFWTNL
                     PKDGVAGEDFLEGGGGPLFPFGIGGIFKALPFAIWFFLAIEEVPLAAEESMDPRRDVP
                     RGSVLAMHTLVIAAVLTLFINTGLPGGASLYGASGFPLLDGFKAVFSGDKVAYVLGIL
                     FMIGLIASFFTIIYAYGRNTYSLSRAGYFPSFLSITHGERKTPHVALIAGAVVGYAVA
                     VLVFILQEAELGAQIVAALLNMAVFAAVISYILQMVSFVLLRQKLPNIERPYVSKLGV
                     PGAVVAGVLAATALIAIFLNEAYRPGVYGVAIYYVLGVLYFAVAGRNRLVLSPEEEFA
                     LTQGEQGVPQATYETSAAAQEAILRGGTGQATSQVSEPPPPPPASG"
     gene            complement(5267..5542)
                     /locus_tag="VF195_06680"
     CDS             complement(5267..5542)
                     /locus_tag="VF195_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580537.1"
                     /translation="MTVLGAPLAVGGPNLVSGEGWDVAPFVLVAALLILMRWARIPLH
                     TPAVCAAIAVGVLLDTVIDASRMSFPVALAVLTLAFGIVALRRTPAR"
     gene            complement(5580..6359)
                     /locus_tag="VF195_06685"
     CDS             complement(5580..6359)
                     /locus_tag="VF195_06685"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX6580538.1"
                     /translation="MGRLADKVALITGGASGMGLIAAQLFASEGAKVVLTDVTDDAGE
                     QVAEKIRTDGGEATYVHADVSREADAEAMVARAVDSYGGLTVLYNNAGVMLGDDGSVH
                     STDESIWDTTLAVNVKGVAHGCKYGIPAMIDSGGGSIINVASFVAWMGAATSQTAYTA
                     SKGAVLAMTREIAVEYARKGVRCNALCPGPIDTPLLAELLSDPERRRRRFVHIPMGRL
                     GRAEELANAALFLASDDSSYMTGTSLVVDGGITAAYVTPED"
     gene            6499..7035
                     /gene="greA"
                     /locus_tag="VF195_06690"
     CDS             6499..7035
                     /gene="greA"
                     /locus_tag="VF195_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX6580539.1"
                     /translation="MPELPEELKTRPERKAGQEPTALLTLEAYNRLKTELQELVTNGR
                     DRIAERLKVAREHGDIRENAEYDAAKDAQGLMESQIRRLQEALRDPEIIEAPMAADAI
                     VPHMLVTVRSLDDGDDEETYLLAEHGEEKAPGARTVTTSSPLGTALMGASKDQEVLVQ
                     APGGSFRYVVVGFEPFVG"
     gene            7049..7723
                     /locus_tag="VF195_06695"
     CDS             7049..7723
                     /locus_tag="VF195_06695"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX6580540.1"
                     /translation="MGRRAAAGHRSRPHVAREAPKVRDLPKPRKNESAAGGAIQWAFQ
                     WPYRAILAFLIWARVRPWHLTVAALVLNVATGVLIITGDWFLAGWTLLAAGLCDVFDG
                     SVARHYGQQKRSGAFMDSVLDRVADAILFACLFWALAEDARRLDAALALFTLVVSLSV
                     SHIRAEAEAVGVQLTEGFFQRLERFIALIIGLVVPGAMTPILLVLAALGAVTALQRGW
                     SALVRA"
     gene            7728..8060
                     /locus_tag="VF195_06700"
     CDS             7728..8060
                     /locus_tag="VF195_06700"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6580541.1"
                     /translation="MEFVRVASLAEIPEGEMRAFDLASGRVAVAHVEARLFAFADECT
                     HAGCALSEGDFDDRAATVECPCHGSVFDVETGEPVDGPAQDPVAVHLARLVDGWVELA
                     DQPVREGG"
     gene            8148..9551
                     /locus_tag="VF195_06705"
     CDS             8148..9551
                     /locus_tag="VF195_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HEX6580542.1"
                     /translation="MTDAPIRFDLPHDQLPTAWLNIMPSIAQAGIQPLPPLHPGTHDP
                     VTPDLLAPLFPEALILQEVSTDPWIDIPGEILDVYRLWRPSPLQRAVRLEKALQTTAR
                     IYYKYEGVSPAGSHKPNTAIAQAFYNKEAGTKRLVTETGAGQWGSALAMASKFFGLDC
                     QVFMVRASYEQKPYRRIFMETFGAEVVPSPSPTTQAGKAILEADPASTGSLGIAISEA
                     VEVAATSGGAIKYSLGSVLNHVLLHQTVIGLEAKAQMELTGDRPDVVIGCVGGGSNYA
                     GLAYPFMVDRLSGATPETRFLATEPAACPTLTKGRFAYDFADTGEMTPLVPMYTLGHT
                     FVPAPVHAGGLRYHGDAPSLSLLVKHGHMEAAAFSQNAVFDAAVQFAHTEGIVPAPES
                     AHAVRGAIDEAIAARDAGEERVILFGLSGHGAFDMQAYDDYLNGRLPEVEYRQEDVTA
                     SMANVPDVPIPGGPTGS"
     gene            complement(9548..10630)
                     /locus_tag="VF195_06710"
     CDS             complement(9548..10630)
                     /locus_tag="VF195_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEX6580543.1"
                     /translation="MSESRGPDEVASAAEAPGLAVKVLYGRRLMPAGRVLVVLLVSLL
                     TWTLLYAPALKRAAEASPIGTRRTLSLAILTPISAMSDWIGLDQLAATIERAVGHDAG
                     KPDGAFVPPPEDIPVAPPDDPDDGAGDGGGENEPIRVPSPARKLRVAIVGDSLAGGLG
                     YFAERVFRPRLVTVSGQGRISTGLARPDYFNWPFTMRRIVDRFDPDLVIVMLGENDHQ
                     SLQDVHGNLEALIGTAGWPPMYRDRVLAMMRIATSSGAKVVWAGLPISADYRLREHSR
                     RQNDLFEFAASISSDVVYFDSWNRFRDPAGGYTAYFREGNRVILIREGDGLHFNAVGY
                     TVVAREIAKLAAEEFGLSPRTFETEI"
     gene            complement(10644..12071)
                     /locus_tag="VF195_06715"
     CDS             complement(10644..12071)
                     /locus_tag="VF195_06715"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX6580544.1"
                     /translation="MLFPTVDFAIFFVVVFLGHWLLNHLVQPWNLFMIAASYVFYAWW
                     DPRFVLLLVVISFIAQLGAIAVDRTRSPRSRLVVNAAAVALLLLPLAFFKYYGFFAVN
                     VTNTLVALGMGSSIPLIQVVLPVGISFFTFMAIAYVVDVYRGDVEVAGWTDTFLYLSF
                     FPHLVAGPIVRPGELIPQLQERRDERHIDVAGAAWLILGGLFKKVVVSSYLAAEIVDP
                     VFGDPFTRSAPDALFGIVGYAIVIYADFSGYTDIAIGSAKLLGFAFPRNFDRPYTARS
                     IQEFWRRWHMTLSRWLRDYLYIPLGGSRRGERRTLINVLVTMVLGGLWHGAAWTFVFW
                     GAFHGGLLAANQWRRRRAPGEGSEERPDRWPDHLRRGAATFALVCVGWVFFRADSLET
                     AFGLLGRLVTGWFTPTEFVNPLVMLTILGMLALQFWPRGFGLWLQAGLSRLKPAPLGI
                     VLALVLLVIVILGPPGVAPFIYFQF"
     gene            complement(12159..13082)
                     /locus_tag="VF195_06720"
     CDS             complement(12159..13082)
                     /locus_tag="VF195_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6580545.1"
                     /translation="MHNVIDRRILLAFAGVVLIAGSNIVAVRFSNRELDPFWGAGIRF
                     LGASVILWVLASRARHPLPRGKALAGAVAYGVLSFLIAYAFFYWGSQEVPAGLGGTVM
                     ASVPLLTVLLAATHRLERLRLRAIAGAVVALAGITLMSSDALSGDVSVLSVLAVVVAA
                     AAAAESGIVLKLLTSAHPVLTNAIGMSVGAVLLLLLSLFARESWNTPTSPSVWVALGY
                     LIVASPFLVMLIVYVIGRWTATGASYQFVLFPIVAVIGGALLLGEEVTSSLLLGAPLV
                     LAGVYIGALAVDRTPQDAYAPRRERTGTTDR"
     gene            13172..13363
                     /locus_tag="VF195_06725"
     CDS             13172..13363
                     /locus_tag="VF195_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580546.1"
                     /translation="MSRDIDWHVFDKAADITASAVRGAMGSEGSQPATYVGEVFGEIY
                     AALRKATDEMPSKDSKPGF"
     gene            13368..14231
                     /locus_tag="VF195_06730"
     CDS             13368..14231
                     /locus_tag="VF195_06730"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX6580547.1"
                     /translation="MDVERSRRAVLDWYHSRRHAYPWRRGRRNAYRTLVSEVMLQQTQ
                     ASRVEPIFDSFVARFPDVGGLGEASRADVLKAWAGLGYNRRAVALHETARVVVREHGG
                     RVPRDVGSLTQLPGVGPYTAAAVASIGHGVAVAALDGNARRVVARAIRGAEPDEVPAG
                     DLARDAGMWLDPSAPGAWNQALMDIGRLFCRPAPRCDDCPVASDCRFLGAGRRGRSSG
                     RRQSTFEGSPRQLRGAVVRILRTVSSASTEVLAERTGRSVAAVATATAALTAEGIVER
                     TRSGWFRLPRR"
     gene            14264..14599
                     /locus_tag="VF195_06735"
     CDS             14264..14599
                     /locus_tag="VF195_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6580548.1"
                     /translation="MEAVLKAIAEPRRRQILRLVWDAERSAGDIASHFDVSRPAISQH
                     LTVLRTVGLVTERRDGARRLYRANRDALAELQASLEAFWTGSLAALKDEVEAEEGRRR
                     GRRHVHRQQ"
     gene            14568..15014
                     /locus_tag="VF195_06740"
     CDS             14568..15014
                     /locus_tag="VF195_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004675541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX6580549.1"
                     /translation="MAAGTFTVNSEIRIDAAPEAIFPFLTDPQKIVRWKGVDATVEAS
                     PGGAYRVNVTGVKHAVGEYVEVDAPHRVVFTWGWEGDEQLPPGSSTVTIELIPDGDGT
                     IVRLTHSGLPEGADAAQLQGWEHFLPRLAIVASGGDPGPDPWVAAR"
     gene            15161..15955
                     /locus_tag="VF195_06745"
     CDS             15161..15955
                     /locus_tag="VF195_06745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEX6580550.1"
                     /translation="MGAEPAAHAQAHLPADEEALLRALNLSFDYGQGSVLRDVTLTVR
                     TGEFVALVGPNGSGKSTLLKLLLGALPPGSGRVELLGRAPGDVRDRWRVGYVPQRPSL
                     LSEVPATVEEIVSAGRLRQRGWWRGLTGSDREAVRHAIESVGLGTLADRPLNELSIGQ
                     QQRAFIARAFAGEPALLVLDEPIAGVDAESQRRFRDSLVHLIHQHGAGVLLVSHELAA
                     VADVIDRVVVLKERVVFDGSPEELAARGVSLGIHREDLPLWLEDLG"
     gene            15952..16797
                     /locus_tag="VF195_06750"
     CDS             15952..16797
                     /locus_tag="VF195_06750"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX6580551.1"
                     /translation="MIGTLPIPYPFESGETQRALVACLAVGVFAPMIGAFLVQKRLSL
                     VGDGIAHVAFAGVGAGLLVGVWPVWSALAFAVTGAIGVEMLRLKRKAAGDLALALFFY
                     VGIAMGAVFASRAGDLELETLPYLFGDPYAVTWTGVALVAALGVIVVVAMRLAGRALF
                     AVVTDEDWSRVAGLPVGALSMALAALTAGVIVAGMRVVGILLIAAMMVLPVASAQLVS
                     RSFRGTLRLAVAIGAVSSLVGLTIARPLDLAPGGSIVFVAAALFAAIALAKRTAPRAL
                     QQEAH"
     gene            16797..17231
                     /locus_tag="VF195_06755"
     CDS             16797..17231
                     /locus_tag="VF195_06755"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX6580552.1"
                     /translation="MELHQEAAERLASSGRRYTRTRRRLVDILAKSGMPLSLPAIVRG
                     RKDLPQSSAYRNLAELEAAGVIRRVASEDGFARYELAEELTGHHHHLMCSRCGQVQDL
                     HIPAPFERRLDRTLDVLAREAAFATVRHRLDLIGLCRACGST"
     gene            17337..20729
                     /locus_tag="VF195_06760"
     CDS             17337..20729
                     /locus_tag="VF195_06760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580553.1"
                     /translation="MPPPATRSGRLGSWWVIVGFALIILLALGWRFIADPSLSAPTRD
                     PAWYTWRAGLILEDDPGSIAGDWGPDNLFSGGYRVTVPLAGALLQRVSGISTYSFSAF
                     LMLGIPILTGLALGAGAFRSRRDPLIVLLTMLATAGLFLTTPYVGYLDNITVLFLLSL
                     IVAFADAARTSWGARVAIFLIGMAAAFTHPTTCVLFGLSLLAVWGIHVVTSRFRFGSA
                     MRSDGPLLLSTGSGMVTGLALWVVGIWGPGGPELLKDAALPPPYTAAFFRERLIEWVT
                     SMRPAITFPLILLAIVATILASRRTRTPAPQYEQVSIWWMFPFAGVLTFLLGQAIPYY
                     RFMNATAAPMALTGLGAYVAVRWLLRGEGSRRLAGALGALAVVGALAWLFLDPAINRW
                     AQQDNQWAPQSVRTSLAATREVVVAAGERPSVLIVNYGNTDDATGSNTTYGWAKTYTN
                     VFRTGLPGEFAKYQVTFVGTVEDFRRGIPSDGPSENYRDTSRSHRDELRARIDTYTEP
                     PVVFLIEEYYLGLCNGLAAGACSPETERAALGRALEDSVEIGPGTHVLTGDRTYEPPA
                     EVVERARSAADAAAARFGDPPGPLADLPHQARVLAGLFALAVLPGLLAAPFFGLKDTV
                     SRIALIPGTSIVLSLLSGIAVLGVWRGPLTDTKAWVVVGAAVGAGAALRYGSASILRA
                     LGAFGGFFNRLFSQFSNAAYASLMSVQFLTQAGQGMIQGAIGKSIAFGGQEGFDVQNV
                     PSADYLLQVILLLYIPYTLLSPFIGVVIDRFERRRVVWWSNLVTAGVVGVVAIAVLLP
                     LGEGTTEGDAGATAALVLGLLAAQAVIRVALAVKSAAIPDVLSGRDLLQGNALSQAGG
                     ALFQILGIAVALAAGGFLSAWLVVLGGAAVLAIGALAATRLRHVEARAHVTRFAEEAR
                     RVLRTIARGVREVAARPAAAVGLSGFQMLRYQFWGFGLFVFALYAKNLVEGGPESQVP
                     TLLSGGVGLIGGVLGLIVAQGLKDRVAPIRLLLVSMALLGAGTLVFGGLVSVVGFAAM
                     LFIGFFAFFLGKVSADTIVQQAMPDDFRGRAFALFDIAYNLGFIVPALVLSLVWVEND
                     AGRTRVVLMTSGAVFLLLTGVVAAWARRIRDQFATQDDLIEA"
     gene            20744..21520
                     /locus_tag="VF195_06765"
     CDS             20744..21520
                     /locus_tag="VF195_06765"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580554.1"
                     /translation="MPEVCNGDVTLHVEIDGAGEPVTIFAHGLTNSCRELAPFTPSLA
                     GTAVRFCFRGHGHSSAPRQGYRFADLASDLDVVARTYGATNAVGTSLGAGAIMSLLEH
                     DPARFDRIVMLLPASLDLPFRNPERFDAIADLLEAYPKDEAIERVLEMNEERYARAPW
                     LRQLDLMLWEDMNPAGVSRAIREVTRDISISDRELLRKVEAPVLLICREGDSIHPAAL
                     GRILADLLPDAELITFNSEEELIASIPSLVERVRAFLEGV"
     gene            21517..22440
                     /locus_tag="VF195_06770"
     CDS             21517..22440
                     /locus_tag="VF195_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6580555.1"
                     /translation="MSESEAHDRRARTPRQELLGSTLSALMALQFAAVVMFGKEVAAG
                     DLPFPMLAIRFAGQAMLLFVVLLLWRKPLAPAPGERLPLVLAATIGYGTEAALFFSAL
                     NHGKAGAVTLLFYLYPVWVMLVTIGLDRRPPGWRLFVALLLALGGSAVVIVGGGEVEV
                     APLGIVLALATSVAYTAYLITADRTVRRTDPVTAAAWLGAGAACSNVVFSLAFGVKDL
                     PDASSWPFLLGMAVFSAGAFAAMLGGLQLLGAVRNAIIGVMEPLGVAVLASFFLDEPI
                     TAPTAIGGVMILGGAILATLVRTTRVREPDV"
     gene            22453..23166
                     /locus_tag="VF195_06775"
     CDS             22453..23166
                     /locus_tag="VF195_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020384933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HEX6580556.1"
                     /translation="MTDQLFAEDAYLTSCEATVVDIRDEGVILDRTVFYARGGGQPGD
                     TGTLRWENGTARVTDTVKMGGDIVHQVEGDAPLVGHSVTAEIDWARRHLLMRTHTALH
                     ALSGVIFREHGAKVTGGNMDDRGSARMDFELDEMSQELGKDVEGKLNEELNRNRPVHV
                     SFLPRAEALSDPDLIRTKVSLIPDTVDPIRVIDIEGIDKQADGGTHVKATGEVGHVTV
                     VKTESKGKAFKRMRIQLEA"
     gene            complement(23170..25104)
                     /locus_tag="VF195_06780"
     CDS             complement(23170..25104)
                     /locus_tag="VF195_06780"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX6580557.1"
                     /translation="MRRLATAPALAALLLMMPGPAEAQDPADEASYGGSVSAIDNLFT
                     PEIVRIDLGETVEWTMDGRSPHTVEADDGSWSSEDLDPGAEFDHTFDTEGIFPFFCRY
                     HGRPGLGMAGTVVVGDAPLPGGGAPGPDPAPVGPANTVRVPDDAPTIQNAVDRARPGG
                     LVLIDPGVYREAVVVTTPYLTIRGVDRNDTILEGNFELANGIHVIEADGVAVENLTAR
                     HFLLNGFYWSDVLGYRGSYLTASTNGDYGIYAFGSRWGRFDHSYASGSPDAGFYIGAC
                     NPCDAVITDVLAEHNALGYSGTNAGGNLAIVNSEWRENLAGIVPNTLDSEPYAPQRDV
                     LIAGNHVHHNNSTTVDAKEYEYVPFGMGIVVAGGRDDRIVENLVEDQATYGIAMIPIV
                     DTNFWPSGGNEVRDNVVRRSGRADLALAAPSAGADCFSGNDVDTSHPAAVELLFPCSG
                     FRPFPGGGGSMGPTANVLSRFIDSLDGEFPHGDWRAQPDPPAGLPDMPGDPAAAPPDP
                     AIPEEAVPARYRIRALGDVRAARGSEISQEVSLMGVPLIASSWWSLLLGLYGYILPFV
                     LYASWVAIAMWDLIRRDADAIPERARWMAIVLLVPFAGPLLYFAVGRSSIPVQLRLML
                     TAGGVIAYLVFLVIGLLVGG"
     gene            25129..>26137
                     /locus_tag="VF195_06785"
     CDS             25129..>26137
                     /locus_tag="VF195_06785"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX6580558.1"
                     /translation="MPAERLSGLDASFLYLEKPGVHMHVAGLSIFGARADGSLLTCDE
                     VERVVEARLRLAPRLRRKLQSVPGNLSRPVWIDDDRFDLDFHLRRASIPAPGGRFELE
                     RAVGRVLSRPLDRTKPLWELYVFEDLSEDRTAILLKIHHAMADGIGNMMIASALFDLT
                     ADAPTGEPDGAWSAEPPPPMQDLIRDAVEDAVLHPIQSLAHVARQPRIVAETVSATAD
                     ALRTVAGMGTAPRGPFDGRVGPNRRFATAERPFAVFRGVKESLGGTVNDVVLAVVAGG
                     VHALLESRGETTRGRSLRAMVPVSVRSPGDGGDVGNRVAPIFVDVPVGRMAARTRLRR
                     VR"
BASE COUNT         3979 a         8871 c         9147 g         4140 t
ORIGIN      
        1 gtggccgctc gagaagatcg ccgaagcgtt cgagacgatc cgcgcgggta ccgggctgaa
       61 gatggtcgtc atgccgtgag ggccatccga gggatggggc cgccgtgaac gactcgttcg
      121 tgatcggttg cgacgtgggc agccagggca cgaacgccgc gctctacggg gctgacggcg
      181 agctggtggc gtcggcgtac gaggcctacg acctctcgtt ccctcacccg ggctgggccg
      241 agcaggaccc ggacctgtgg acgtccgcga tcgagcgcgc ctgtcggcgt ctggtctcgg
      301 agtgccccgg cggagcggcc gcgatccgcg gcctctcctt cggatcccaa ctcgacggca
      361 tggtggtctg cgacgcatcg gggcgccgcc tgcgcgccgc gctgatctgg atggaccgcc
      421 gggccgaggc gcaggcggcg gacctcgccg aacggatctc gggtgaggac ttctaccggg
      481 ccgtcggcgc gaacctcgac tcgtcccacg ccgtcttcaa ggcgctctgg gtccgcgacg
      541 aggagcctga gctgtggcgc gaggccgcgc gcctgatgtc gccgggcacg tacgtgctcc
      601 gacacgcgac cggcgtgacc gccgtcgacc cgtcgaacgc gtcgtcgatg gccctcctcg
      661 atccacggac gaagacctgg tcgccccagg tgctcgaggc gacggagatc gaccgggcga
      721 tgcttcccga gctcggggac gccacgaggg cagtcgggac cgtgacgtct gcgttcgcgg
      781 aggcgagcgg gctcgcgccc gagacggtcg tcgcggtcgg gtgcggagac gagatggccg
      841 cgacgctggg cgccggcgtg ttcgagcccg gtgaggtctg cgacgtcgtc ggcaccgccg
      901 agccggtatg cgccgcgagc gccgagccac gcgaggatcc gacgatgttg gtggagtgcc
      961 atccgcacgc ggatcccgac gcgtggctcc tcgagaaccc ggggttcgtc tctggtggca
     1021 accttcgctg gtggcgtgac cagttcgccc cggacgagcg ccggaacgag gaacaggggc
     1081 tgggggacgc ctacgatcag ctgagcgagg aggcgtcgcg gatcgcgccc gggagcgacg
     1141 gcgtcgtctt ccttccggcc atgcaggggg cgatggcccc cgaatggaac ggcgccgccc
     1201 ggggtgtttt cttcggactc accctcgccc acacgcgcga tcacatgacg cgggcgatcc
     1261 tcgaggggag cgcgttcggc ctccgcgaca tcctcgaggc gatgaaggct gccggcctcg
     1321 gcgtgcggcg tctcacgatc gtgggaggtg gggccaaggg gccgctgtgg cggcagatca
     1381 aggcggacgt cacgggcctc ccggttcggg tcccggagag cgtcgagacc accgccaccg
     1441 gcgccgcgat cctcgccgcc gtcggctccg gcgtgcacgc gaccgtggca gacgcggtgg
     1501 attcgttcgt gtcgttccgg ccggaggagc acgagcccga tccggcggct cgcgacgcgt
     1561 acgacgaggc ctatcagcgc taccgcgatg tgtacttcgc tctgaagcct gtgttcggac
     1621 cagactgagc gggatcagtc ctcgagctcg cgtgcgacgt cgtcgcggag gttcgccaga
     1681 cggtcgatga cggtccggat cgcgtcacgg atcccttcgg gggcgctgtc cacgagtccc
     1741 tcgagctcct cacgagcatc actcatccgc cgctctgcat cctcgagcgc ttgtcgcgtc
     1801 tcggcgttgg ggttctcgcg gtacgcctcc agggcggagt ccgcctcgtc gacggtgtca
     1861 cggacccggg cgacggcctc gcggaccggc ccctcgtccc cgatcgcgtc gacgagatcg
     1921 tcgatcgcgt tcgtggcatc ccggaactcg tccgtgaggc cgctctcgga gaagcatgcg
     1981 gacagcagcg cacacagcac gatcaagctg gctctcttca tgtccgaccc gtacccgcgg
     2041 acgggcgtcg gcaagcatca gccgcgttcg aagtaccgga tcagctccca atcggtgacg
     2101 gtctggttgt cgaagatgat ctgctcctgg cgggccatgt tcaccagatg cccgaacgcg
     2161 aagtcgccga aggcttcccg catcacctcc gagcgctcca tctccgcgat cgcctcgtgc
     2221 agcgaggacg gcacccgctc ggcgtccttc gactcgtagg cattcccttc gaacagggga
     2281 gggcactcga tctggttctg catcccgtgg agaccggccg cgatcgttgc cgcgtacccg
     2341 aggtacgggt tgacgtctcc gcccggcagc cgcgactcga cccggttgcc ctgatgctcg
     2401 ccgaccacgc ggaagccgca cgtacggttg tcgtgactcc acaggatcgc ggtcggcgcc
     2461 cacgatccga gttggtagcg cttgtaggag ttcacgaacg gcgcgtacat ccatgccatc
     2521 tcacgcgagg tggacatcag cccccccagc atgtgcttga acgtcggact catgtggttc
     2581 gggtcgtcgt cgtcccacat gagtgattcg gagccgtcct cgttccacac gctcgagtgc
     2641 atgtggcaag aggagcccgc ttcgtccatc gtccacttcg ccatgaaggt gatcgcggcg
     2701 ccgttcatgg cggcgatctc cttcgtcccg tgcttgtaca gcgcgtggta gtcggcggtc
     2761 gtcagggcgt ccgcgtacgt gatgttgatc tcgtgttggc cacgaccgaa ctcccccttc
     2821 gagaactcga tggggatccc cgcgcccaac atcccgttgc ggatctgccg gatcagccac
     2881 tcgtccttgg tggtcgcgag catgtggtag tccatgatgt acggggacga cggctggatg
     2941 ccctggtagc gcttcgcagc ggcctcttcg taggattcct tgaagaggta gaactcgagc
     3001 tccgatccgg tcttgaacgt gtagccggcc gccttcgccc gctcggcctg gcggcggagg
     3061 atcgtgcggg gcgcgacctc gaccagctcg ctcgtgtgct cgtccgcgac gtcgcagatc
     3121 accatcgccg tcttctccag ccacgggacg agtcgcatcg tcgacatgtc cgggatcatc
     3181 ttgaagtcgc cgtagccggt ctcccagctc gcgtactcga agccgggcag tggctccatc
     3241 tccatatcga tcgcgatcag atacaggcac gcgtggagcc cttcgtctcc catcacgtcc
     3301 tcgaggaaga agtgcgggag gatccgcttg ccgacgaacc ggccctggaa gtccgtgaac
     3361 atgcacagga cggtgtcgat ctcaccggcg tcggcgagct cctcgagctg cttcgcgtcg
     3421 atcatgccct gctgttgcac gtgccctccc cggagatgag gaggggcggc gggcttgatg
     3481 cccaccgccc ctcgagcgtc gagtgacctt gctacccgga ggcgggcgga ggcggcggcg
     3541 gctccgacac ctgcgaggtc gcttgccctg tgcctccgcg gaggatggcc tcctgtgcgg
     3601 cggcgctcgt ctcgtatgtc gcttgcggta cgccttgctc gccctgcgtg agcgcgaact
     3661 cctcctccgg cgagagcacc agtcggttcc ggccggcgac ggcgaagtac agcacgccga
     3721 gcacgtagta gatcgcgacg ccgtacaccc ccggtcgata ggcctcgttc aggaagatcg
     3781 cgatcagggc tgtcgcggcg agcacacctg cgacgaccgc tccggggacc cccaacttgc
     3841 tgacgtaggg ccgctcgatg ttcggcagct tctgccgcag tagcacgaac gacaccatct
     3901 gcaagatgta cgagatcacg gccgcgaaca ccgccatgtt gagcagtgcc gccacgatct
     3961 gcgcacccag ttcggcttcc tggaggatga acacgagcac cgccacggcg tagccgacaa
     4021 cagctccggc gatcagcgcc acgtgggggg tcttccgctc accatgggtg atcgagagga
     4081 acgaagggaa gtatcccgcc cgtgacagcg agtacgtgtt tcgcccgtac gcgtagatga
     4141 tcgtgaagaa gctcgcgatc aggccgatca tgaagaggat gcccaggacg tatgcgacct
     4201 tgtcgcccga gaaaaccgcc ttgaagccgt cgaggagcgg gaaaccggac gcaccgtaga
     4261 gcgaagcccc gccgggcagc cccgtgttga tgaatagcgt gagcacggcc gcgatcacca
     4321 gcgtatgcat cgcgagcacc gagccccggg ggacgtcccg ccgggggtcc atcgactcct
     4381 ccgcggccaa ggggacctct tcgatcgcga ggaagaacca gatcgcgaac gggagggcct
     4441 tgaagatgcc accgatcccg aacgggaata acggccctcc accaccctcg aggaagtctt
     4501 cgccggcgac gccgtccttc ggaaggttcg tccagaagct cgggtcgaac ttcccggaca
     4561 cgagggcagc gatgaagaag aacgccagaa caacgagaga gaggaccgtg atcccgacgg
     4621 tgaacttcat cgtggcttcg atgccgatga tgttgatccc cacgaagatc acgtagaaga
     4681 cggcccacca gaacgggagg ctgttccacc aggcgatgtc ggtgatcgat ccgtcagcag
     4741 cgaaggtgcc gccgacctcg aacaggccga ccacgatctg ctgcatcagc aggcccatcg
     4801 cgcccacgac aacggccggc gtgatgacgt actccatgtt ctccgcgagg ccggtgagga
     4861 acccgcccca ggggcccatc gccgatcgtg cgaaggaata cgccccgccc gtgtgcggga
     4921 gggccggaga catctcggcg atcgagtaca cgaggccgta gtacatgatc gccatgatca
     4981 cgccggcgac cagcaggcct ccgaatcccc cggttccgag gccgaagttc catccgtagt
     5041 actcgccaga gatcacggct ccgacgccga gcgcccacag cgacgtgacg ccggcatgtc
     5101 gccgcaagcc ccgcttgtcg aagtagtcct cacctacctc gcgataggtg acggagccga
     5161 gcttccgttc ggacatggca accctccctg gagccgaccc cgtgagcggc tccggcgggt
     5221 atcgagccat aggtacggtt tgtctgtcaa acctgcgatc tccgggtcaa cgggccggag
     5281 tgcgtcgcag cgcaacgatc ccgaaagcga gggtcagaac ggccagcgcg accgggaacg
     5341 acatcctaga tgcatcgatc accgtgtcga ggagcacgcc aacggcgatg gcggcgcaca
     5401 cggcgggggt gtgcagcggg atccgggccc accgcatcag gatcaggagc gctgccacga
     5461 ggacgaatgg agcaacgtcc cagccttcgc ccgagacgag gttcggcccg ccgacggcga
     5521 gcggagctcc gagaacggtc acggagccag gctaccgcca gggcctcccg gtaaggggtt
     5581 caatcctcgg gcgtgacgta ggcggccgtg atcccgccgt cgacgaccag cgacgtgccc
     5641 gtcatgtagc tggagtcgtc gctcgcgagg aacagggccg cgttcgcgag ttcctccgcg
     5701 cgtcccaggc gacccatcgg gatgtggacg aatcgacgcc gccgtcgctc cgggtccgaa
     5761 aggagctcgg ccagcagggg agtgtcgatc gggccgggac acaaggcgtt gcagcgcacg
     5821 cccttcctcg cgtattcgac cgcgatctct cgcgtcatcg cgagaaccgc tcctttcgag
     5881 gccgtatacg ccgtctggga cgtcgcggcc cccatccagg cgacgaagga ggcgacgttg
     5941 atgatcgaac cacctccgga gtcgatcatc gccgggatgc cgtacttgca gccgtgcgcg
     6001 acccccttca cgttgaccgc gagggtcgtg tcccagatgg actcgtcggt ggaatggacc
     6061 gagccgtcgt cgccgagcat cacccccgcg ttgttgtaga ggaccgtgag acccccgtac
     6121 gaatcgacgg cccgcgccac catcgcctcc gcgtccgcct cgcgggacac gtccgcatgg
     6181 acgtatgtcg cttcgccccc gtccgtgcgg atcttctcgg cgacctgctc accggcgtcg
     6241 tcggtcacgt cggtcaggac caccttggcg ccttcggacg cgaagagctg agccgcgatc
     6301 agacccatcc cgctcgcgcc tcccgtgatc agcgccacct tgtctgccag gcgccccatg
     6361 gatacctccg ttctttctga ccgttctttc tgacccgatc cggtgcggcg aggatatcgt
     6421 tgccggtctg gactggcgct ccgcacggtc cgagtggctc gccggagagg cccacggggc
     6481 cttcagtaga ctcgcgccat gcctgaactg ccagaagagc tgaagacgcg tcccgaacgc
     6541 aaggccgggc aggagccgac ggcgttgctg acgctcgagg cctacaaccg gctgaagacc
     6601 gagctccagg agctcgtgac caacggacgt gaccggatcg cggagcgcct caaggtggcg
     6661 cgcgagcatg gggacatccg ggagaacgcc gagtacgacg cggcgaagga cgcgcagggc
     6721 ctgatggaat cgcagatccg ccggctccag gaggcgctcc gcgatccgga gatcatcgag
     6781 gcaccgatgg cggcggatgc gatcgttccg cacatgctcg tcactgtgcg ttcgctcgac
     6841 gacggcgacg acgaggagac ttacctcctg gcagagcacg gcgaggagaa ggcaccgggt
     6901 gcgcgaacgg tcacgacctc gtcccctctc gggacggccc tgatgggcgc gtcgaaggat
     6961 caggaggtgc tggtgcaggc gccggggggg agcttccggt acgtggtcgt cggcttcgag
     7021 ccgttcgtcg gttgatgagc gcgagctgat gggccggcgc gcggccgcgg ggcatcgctc
     7081 gagaccccac gtcgcgcgcg aggcgccgaa ggtccgcgac ctcccgaagc ctcggaagaa
     7141 cgaatcggcg gccggcggcg cgatccagtg ggcgttccag tggccgtacc gggcgatcct
     7201 cgcgttcctc atctgggcac gggtgcgacc gtggcacctg acggtcgcgg cgctcgtcct
     7261 caacgtggcg acgggggtgc tgatcatcac gggggactgg ttcctcgccg gatggaccct
     7321 gctcgcggcc ggcctgtgcg acgtcttcga cgggtcggtc gcgaggcact acgggcagca
     7381 gaagcggtcg ggggcgttca tggactcggt cctggaccgc gtcgccgacg cgatcctgtt
     7441 cgcctgcctg ttctgggcgc tcgcggagga tgcgaggcgc ctcgacgccg ccctcgccct
     7501 cttcaccctg gtggtgagcc tgtccgtgag ccacatccga gccgaggccg aagctgtcgg
     7561 tgtgcagctc accgagggct tcttccaacg gctggagcgg ttcatcgcgc tgatcatcgg
     7621 gctcgtcgtt ccgggagcga tgaccccgat cctgttggtt ctcgccgcgc tcggcgcggt
     7681 gacggcgctc cagcgcggct ggtccgcgct cgtgcgagcc taggccgatg gagttcgtgc
     7741 gcgtcgcttc gctcgccgag atccccgagg gcgagatgcg ggcgttcgat ctggcctccg
     7801 gacgggtggc cgtcgcgcat gtcgaagcga gactgttcgc gttcgccgac gagtgcaccc
     7861 atgcggggtg cgctctctcg gagggcgact tcgacgatcg ggcggccacc gtcgagtgtc
     7921 cgtgccacgg gagcgtcttc gacgtggaga cgggagagcc ggtcgacggt cccgctcagg
     7981 acccggtcgc ggtccatctg gcccggctcg tggacgggtg ggtggagctc gccgatcagc
     8041 cggtccgcga gggcggctga cccgggactg gctcccggaa cgcgctccgg ggtaggctcg
     8101 cccctcgtcc ggtttgctgg cttctggcga acgaggaggc ttccccgatg accgacgcgc
     8161 cgatccggtt cgatctcccg cacgatcaac tgccgaccgc gtggctgaac atcatgccct
     8221 cgatcgcgca ggccgggatc cagcccttgc cgcccctgca cccgggaacg cacgaccccg
     8281 tcacgccgga tctcctggcc ccgctgttcc ccgaagcgct gatcctccaa gaggtctcca
     8341 ccgatccgtg gatcgacatc ccgggcgaga tcctcgacgt ctaccggctc tggcggccgt
     8401 ccccgctaca gcgagccgtc cgcctcgaga aggcgctcca gaccacggca cggatctact
     8461 acaagtacga aggagtctcc ccggcgggga gccacaagcc gaacacggcg atcgcgcagg
     8521 ccttctacaa caaggaggcg gggacgaagc gcctggtcac cgagaccggc gccggccagt
     8581 ggggctccgc gctcgcgatg gcgtcgaagt tcttcgggct ggactgccag gtgttcatgg
     8641 tgagggcgtc ctacgagcag aagccgtacc ggcgcatctt catggagacg ttcggtgccg
     8701 aggtcgtgcc ctcgccgtct ccgacgacgc aggccgggaa ggcgatcctc gaagcggatc
     8761 ccgcatcgac cggttccctc ggcatcgcga tcagcgaggc cgtcgaggtc gccgccacct
     8821 ccggcggcgc gatcaagtac tccctcggct cggtgctgaa ccacgtgctg ctgcaccaga
     8881 ccgtgatcgg gctcgaggcg aaggcgcaga tggagctgac gggtgatcgg ccagacgtcg
     8941 tgatcggatg cgtgggcggc ggatcgaact acgccggcct cgcgtacccg ttcatggtcg
     9001 atcggctctc gggtgcgacg ccggagacgc ggttcctggc caccgagccg gcggcgtgtc
     9061 ccacgctgac gaaggggcgg ttcgcctacg acttcgccga tacgggcgag atgacgccgc
     9121 tggtgccgat gtacacgctc ggccacacgt tcgtgccggc cccggtgcac gcgggaggac
     9181 tgcgctacca cggggatgca ccgtcgctct cgctcctcgt caagcacggc cacatggagg
     9241 ccgccgcctt ctcccagaac gcggtgttcg acgcggcggt gcagttcgcc cataccgagg
     9301 ggatcgtgcc ggcccccgag tcggcccatg ccgtccgggg agcgatcgat gaggcgatcg
     9361 cggcgcgcga tgccggagag gagcgcgtga tcctcttcgg gctttcgggt cacggggcgt
     9421 tcgacatgca ggcctacgac gactacctga acggccgcct gcccgaggtg gagtatcggc
     9481 aggaagacgt gacggcatcg atggcgaacg tgcccgacgt accgatcccc gggggaccca
     9541 cagggtccta gatctccgtc tcgaacgtcc gcggcgagag cccgaactcc tccgcggcga
     9601 gcttcgcgat ctcacgcgcg acgaccgtgt acccgacggc gttgaagtgg agaccgtcgc
     9661 cctcccggat caggatcacg cggttgcctt cgcggaagta cgcggtgtat ccgccggcgg
     9721 ggtcgcggaa gcggttccac gagtcgaagt agacgacatc gctggagatg ctcgcggcga
     9781 actcgaagag gtcgttctgc ctcctcgaat gctcgcgcaa ccggtagtcg gcggagatcg
     9841 gcaggccggc ccatacgacc ttcgcgcccg acgacgtcgc gatgcgcatc atcgcgagca
     9901 cgcggtcccg gtacatggga ggccatcccg cggtgccgat cagcgcctcc aggttgccgt
     9961 gcacgtcctg caacgattgg tggtcgttct ctccgagcat cacgatcacg aggtccggat
    10021 cgaaccggtc gacgatccga cgcatcgtga acggccagtt gaagtagtca gggcgtgcga
    10081 gtccggtcga gatcctgcct tggcccgaga cggtcaccag ccgcggccgg aacacgcgct
    10141 cggcgaagta gccgaggccg ccggcgaggg agtcgccgac gatcgccacg cggagcttcc
    10201 gcgccggcga cgggacgcgg atcggttcgt tctccccgcc accatctccc gcgccgtcgt
    10261 ccggatcgtc cggcggcgcg accgggatgt cctcgggcgg gggaacgaac gcgccatccg
    10321 gcttgcccgc gtcgtggccg acggcgcgct cgatcgtggc ggcaagctgg tcgagaccga
    10381 tccaatcgct catcgcggag atcggcgtga ggatcgcgag cgacagcgtg cgccgggttc
    10441 cgatcggcga cgcctctgct gcccgtttca aggccggcgc gtacaggagc gtccacgtga
    10501 ggagagagac caggaggacc acgagcaccc gaccggcggg catcagccgg cggccgtaga
    10561 ggaccttgac ggcgagcccc ggagcctcgg ccgccgacgc gacctcgtct ggtccccgcg
    10621 actcgctcat ctgctcgccc tcttcagaac tggaagtaga tgaagggtgc cactccaggt
    10681 ggccccagga tcacgatcac cagcagaacg agtgccagaa cgatcccgag cggcgcgggc
    10741 ttcagccggg aaagtcccgc ctggagccac aacccgaagc ctcggggcca gaactgcagc
    10801 gcgagcatcc cgaggatcgt cagcatgacg agcgggttca cgaactcggt cggggtgaac
    10861 cagcccgtga cgagccggcc gaggagcccg aacgccgtct cgagcgagtc ggcccggaag
    10921 aagacccatc cgacgcagac gagcgcgaac gtcgccgcgc cccggcggag gtggtccggc
    10981 cagcgatccg gtcgctcctc cgatccctct ccgggggcgc gccgccggcg ccactggttc
    11041 gcggcgagca ggcctccgtg gaacgccccc cagaagacga aggtccacgc tgccccatgc
    11101 cacagaccgc cgagaaccat ggtgacgagg acgttgatca gggtccgccg ttccccccgc
    11161 cggctcccgc cgagcgggat gtacaggtag tcacgcaacc accgggaaag cgtcatgtgc
    11221 cagcgacgcc agaactcctg gatcgagcga gccgtgtacg gccggtcgaa gttcctcggg
    11281 aacgcgaagc cgagcagttt cgcgctcccg atcgcgatgt cggtgtaacc gctgaagtcc
    11341 gcgtagatca cgatcgcgta gccgacgatc ccgaagagcg cgtcgggagc gctcctcgtg
    11401 aacgggtcgc cgaagaccgg gtccacgatc tcggcggcga ggtagctcga gacgacgacc
    11461 ttcttgaaca ggccgcccag gatcagccag gccgcccccg ccacgtcgat gtgacgctcg
    11521 tcccgacgct cctgcagctg ggggatcagc tcgccgggcc gcacgatcgg cccggcgacg
    11581 agatggggga agaacgacag atagaggaac gtgtccgtcc accccgccac ctcgacgtca
    11641 ccccgataga cgtccacgac gtaggcgatc gccatgaacg tgaagaacga gatccccacg
    11701 ggtaggacca cctggatcaa ggggatcgag gatcccatcc cgagggcgac gagcgtgttc
    11761 gtcacgttca ccgcgaagaa cccgtagtac ttgaagaagg cgagcggcag gaggaggagc
    11821 gcgacggcgg cggcgttgac caccagtcgc gagcgagggg aacgcgttcg gtcgaccgcg
    11881 atcgcgccca actgcgcgat gaagctgatg acgaccagga ggaggacgaa ccgcggatcc
    11941 caccacgcgt agaagacgta gctcgccgcg atcatgaaca ggttccaggg ttgcacgagg
    12001 tggttcagca accagtgccc caggaagacg accacgaaga agatcgcgaa gtcgaccgtg
    12061 gggaagagca tcgggaacgg atgctagccg aggtggctcg gcggttccgt tcgacggtga
    12121 tcctgggctc agggagcgcg acgagcgccc ggccggacct acctgtccgt ggtgcccgtt
    12181 ctctcccgcc ggggtgcgta tgcgtcctgc ggggtcctgt cgacggccag cgcaccgatg
    12241 tagaccccgg cgaggacgag tggcgccccc aggaggaggg atgacgtcac ctcttccccg
    12301 agcaacaggg cgccgccgat caccgccacg atcgggaaca ggacgaactg gtagctcgca
    12361 cccgtggccg tccaccgtcc gatcacgtag acgatcagca tgacgaggaa cggcgatgcg
    12421 acgatcagat agccgagggc cacccacacg ctcgggctcg tgggcgtgtt ccacgactcg
    12481 cgagcgaaca gagagagcag gaggagcagc accgcgccca cgctcatccc gatcgcgttc
    12541 gtcaagacgg ggtgggccga cgtcagcagc ttgaggacga tgccggactc cgcggccgcc
    12601 gccgcagcga cgacgaccgc cagcaccgag agcacggaca catcacccga gagtgcgtcg
    12661 gacgacatca gcgtgatacc cgccagcgcg accaccgcgc cggcgatcgc gcggagccgc
    12721 aagcgctcga gccgatgggt cgcggcgagc aggaccgtga gcagagggac cgacgccatc
    12781 acggttcctc ccaggccggc agggacctcc tgggaacccc agtagaagaa ggcgtacgcg
    12841 atcaggaagc tgagcacgcc gtaggcgacc gcgccggcga gcgccttccc ccgagggagc
    12901 gggtggcgcg cccgcgaagc gagcacccag aggatcacgg acgctcccag gaagcggatg
    12961 ccggcgcccc agaacggatc gagctctcga ttgctgaacc gcacggcgac gatgttcgag
    13021 cccgcgatga ggacgacgcc ggcgaaggcg aggaggatcc gccggtcgat cacgttgtgc
    13081 atcgacggat gataggtgcg gatgccgagg cggaggtagc ctgtggcccc accgatcccg
    13141 gcccatccgg cccacggacg caggaggaac catgtcacgc gacatcgact ggcacgtgtt
    13201 cgacaaggcc gccgacatca cggcttccgc ggtgcgaggg gcgatgggct ccgaggggag
    13261 ccagcccgcc acgtacgtgg gtgaggtctt cggcgagatc tacgcggcgc tccggaaggc
    13321 caccgacgag atgccttcga aggactcgaa gcccgggttt tgatcgggtg gacgtcgaac
    13381 ggtcacgtcg ggcggttctg gactggtacc actcccgccg ccacgcgtat ccgtggcggc
    13441 ggggccgccg gaacgcctac cggaccctgg tctccgaggt gatgctccaa cagacgcagg
    13501 cgtctcgcgt cgagccgatc ttcgactcgt tcgtggcgcg cttccccgac gtcggcgggt
    13561 tgggggaggc gtcgcgcgcc gacgtcctca aagcgtgggc cgggctcggt tacaaccggc
    13621 gcgccgtcgc gctgcacgag accgcacgtg tcgtcgtccg cgagcacggg ggacgggtgc
    13681 cgcgagacgt gggctcgttg acccagctcc ccggcgtggg tccgtacacg gccgccgccg
    13741 tcgcatcgat cggccacggg gtcgcggtcg cggcgctgga cggcaacgcg cggcgggtcg
    13801 tcgcgcgggc gatccgaggg gcggagccgg acgaggtccc ggccggtgac ctcgcgcgtg
    13861 acgcgggcat gtggctcgat ccctcggcgc cgggtgcctg gaaccaggcg ttgatggaca
    13921 tcggacgtct cttctgtcga ccggcgccgc ggtgcgacga ctgcccggtg gcttccgact
    13981 gccggttcct cggcgccggc cgacggggca ggtcgtccgg ccgccgacag tccacgttcg
    14041 agggcagccc ccgccagttg cgcggcgcgg tggttcggat cctgcgtacg gtcagctcgg
    14101 cgagcaccga ggtgctcgcc gagcggacgg gccgctccgt cgccgcggtc gccacggcga
    14161 ccgcggcact caccgcggag gggatcgtcg agcgcacgcg atcgggatgg ttccggctcc
    14221 cgcgtcgttg acgctctcgt aagcatctgc ttacatccat cgcatggagg ctgtgctgaa
    14281 ggcgatagcg gagcctcgcc gtcgccagat cctccgcctc gtctgggacg cggagcgctc
    14341 cgccggtgac atcgcctcgc acttcgacgt cagccgcccg gcgatctcgc agcacctgac
    14401 ggtcttgcgc accgtcggcc tcgtcaccga acgtcgagac ggcgcccgcc gcctgtaccg
    14461 agcgaaccgc gacgcgctcg ccgagctgca ggcctcactc gaggcgttct ggacgggtag
    14521 cctcgcggcg ctgaaggacg aagtcgaagc cgaggaaggg agacgacgtg gccgccggca
    14581 cgttcaccgt caacagtgag atcaggatcg acgccgcgcc tgaagccatc ttcccgttcc
    14641 tcacggatcc gcagaagatc gttcggtgga agggggtgga cgccacggta gaggcgtctc
    14701 ccggcggggc gtaccgcgtg aacgtgaccg gtgtgaagca cgcggtcggg gagtacgtcg
    14761 aggtcgacgc acctcaccgc gtggtgttca cctgggggtg ggagggtgac gagcaactcc
    14821 cgccgggctc ctcgaccgtg acgatcgagc tgatccccga cggagatggc acgatcgtgc
    14881 ggctgacgca ttccggcctg ccggaggggg cagacgcggc gcagctccag ggatgggagc
    14941 acttcctgcc gcgactcgcc atcgtcgcga gcggcggcga ccccgggccg gacccctggg
    15001 tggcggcccg ctgacccaac ctgggtggcc tcagcccccg agcgggcgac cgcttcgtcc
    15061 gattgacgga tcgaggctcc gccggtagcg tgaccccatc cgatgagaat gggaatgatt
    15121 ctcaatactc ctgccccgac gctcgtcggt ggaggccgtc atgggagctg agccggccgc
    15181 gcacgcacag gcccacctcc ccgctgacga ggaggcgctc ctccgcgccc tcaacctgtc
    15241 gttcgactac ggccaaggat ccgtactgcg ggacgtcacc ctcacggttc ggaccggcga
    15301 gttcgtggcg ctcgtaggac ccaacgggtc ggggaagtcc acgctcctga agctgctcct
    15361 cggggcgctg ccccccggct ccggtcgcgt cgagctcctg gggcgggccc ccggcgacgt
    15421 ccgcgatcga tggcgcgtgg ggtacgtgcc gcagaggccc tccctgctgt ccgaggtgcc
    15481 tgccaccgtc gaggagatcg tgtccgcggg gaggcttcgc cagcgagggt ggtggcgcgg
    15541 tctcacgggg tcggaccggg aggcggtccg tcacgcgatc gaatccgtgg gcctgggcac
    15601 cctcgccgac cgccctctga acgagctctc tatcgggcaa cagcaacgcg cattcatcgc
    15661 gcgcgccttc gccggcgaac ccgcgctcct cgtcctcgac gagccgatcg cgggcgtgga
    15721 cgcggaatcg caacgacggt tccgggactc gctcgtgcac ctgatccacc agcacggcgc
    15781 cggtgtgctc ctcgtgtcgc acgagctcgc ggcggtggcg gacgtgatcg atcgggtcgt
    15841 cgtgctgaag gagcgcgtgg tgttcgacgg gagccccgag gaactcgccg cgcgaggcgt
    15901 gtcgctcggc atccatcgcg aggacctgcc gctctggctc gaggacctcg gatgatcgga
    15961 acgctcccga tcccgtatcc gttcgagtcg ggagagacgc aacgggcact cgtcgcgtgc
    16021 ctcgccgttg gggtgttcgc tcccatgatc ggcgcgttcc tcgtccagaa gcggctctca
    16081 ctcgtcggcg acgggatcgc gcacgtggcg ttcgcggggg tgggcgccgg tttgctcgtc
    16141 ggggtctggc ccgtgtggag cgctctcgcg ttcgcggtga ccggtgccat cggcgtggag
    16201 atgctccggc tgaagcgcaa ggcggcgggt gacctcgccc tcgcgctctt cttctacgtc
    16261 ggcatcgcga tgggcgccgt gttcgcgagc agagcgggtg acctcgagct cgagacgctc
    16321 ccgtacctgt tcggcgaccc gtacgccgtc acgtggacgg gcgtcgcgct ggtggctgcg
    16381 ctcggcgtga tcgtcgtggt cgcgatgcgc ctcgcgggcc gggcgctgtt cgcggtggtg
    16441 accgatgagg attggtcgcg cgtcgccggc cttcccgtgg gtgctctcag catggcgctg
    16501 gccgcgctca ccgcaggggt gatcgtcgcg ggcatgcggg tcgtgggcat cctgctgatc
    16561 gcggcgatga tggtgctccc cgtcgcgtcg gcgcagctgg tctcgcggtc cttccgcggt
    16621 acgctccggc tggccgtggc gatcggggcc gtgtcctcgc tcgtcggtct cacgatcgcg
    16681 cgaccgctcg acctcgcccc cggcgggtcg atcgtcttcg tcgcggctgc cctgttcgca
    16741 gcgatcgcac tcgcgaagag gaccgcgccg agggcgctcc agcaggaggc ccactgatgg
    16801 agctacatca ggaggcggcg gaacggctcg cgtcgtccgg ccggcggtac acgcggacga
    16861 gacgccgcct ggtggacatc ctcgcgaagt ccggcatgcc actctcgctc ccggcgatcg
    16921 tccgcgggcg caaggatctg ccgcagagct ccgcgtatcg gaacctcgcc gagctcgaag
    16981 cggcgggcgt gatccgccgg gtggcctcgg aggatgggtt cgcgcggtac gagctcgcgg
    17041 aggagctcac cgggcaccat caccacctga tgtgctcgcg ctgcggccag gtccaggatc
    17101 tccacatccc ggcgccgttc gagcgccgat tggatcgaac cctcgacgtg cttgcacgcg
    17161 aagcggcatt cgcgacggtc cgtcaccgcc tcgatctgat cgggctgtgt cgcgcctgcg
    17221 ggagcacgtg accggcggcc gctgacctct atccttgggg gctgtcccgg ccggcgcgct
    17281 gggtcctcgg gaggatcgtg acgctcactc gtaccgctca ggccgacgaa catgccgtgc
    17341 cgccgcccgc tacccgctcg ggccgactcg gctcgtggtg ggtgatcgtg ggcttcgcgc
    17401 tcatcatcct cttggcgctc ggctggcgat tcatcgcgga tccgtccttg tcggctccca
    17461 cccgggatcc cgcttggtac acgtggcggg cgggactgat actggaggac gatcccgggt
    17521 cgatcgccgg cgattggggt ccggacaacc tgttctcggg cggctaccgc gtgacggttc
    17581 cgctcgccgg cgcgctgctg cagagggtca gcgggatcag cacgtacagc ttcagcgcgt
    17641 tcctgatgct ggggatcccg atcctcaccg gtctggctct cggggcgggg gccttccgga
    17701 gccggcgcga tccgctgatc gtgctcctca cgatgctcgc gacggccggg ctcttcctca
    17761 cgaccccgta cgtcgggtat ctcgacaaca tcacggtcct gttcctgctc agcctgatcg
    17821 tcgcgttcgc cgacgccgcg cgcacctcgt ggggcgcccg ggtcgcgatc ttcctgatcg
    17881 ggatggcggc ggcgttcacg catccgacca cctgtgtcct gttcgggctc tcgttgctcg
    17941 cggtgtgggg gatccacgtc gtcacgagcc gcttccgctt cggatcggcg atgcgatcgg
    18001 atggcccgct cctgttgtcg acgggctccg gcatggtcac cgggctcgcg ctctgggtgg
    18061 tgggcatctg ggggcccggc ggtcccgagc tcttgaaaga cgccgcgctc ccgccgccgt
    18121 acaccgcggc cttcttccgg gaacgcctga tcgagtgggt gacctcgatg cggccggcga
    18181 tcacgttccc cctgatcctc ctggcgatcg tggcaaccat cctcgcgtcg agacgcacgc
    18241 gaacccccgc gccgcagtac gagcaggtct cgatctggtg gatgttcccg ttcgcgggtg
    18301 tgctcacgtt cctcctcgga caggcgatcc cgtactaccg cttcatgaac gcgaccgcgg
    18361 caccgatggc gctcaccggc ctgggcgcgt atgtggccgt gcggtggctg ctccgagggg
    18421 agggctcgcg aaggctcgct ggggccctcg gcgcgctggc ggtggtgggc gcgctcgcgt
    18481 ggctgttcct ggaccccgcg atcaaccgat gggcgcagca ggacaaccag tgggctcccc
    18541 agagcgtgcg gacctcgctg gcggcgacac gagaggtggt ggtcgcggcc ggcgagcgac
    18601 cgagcgtcct catcgtgaac tacggcaaca ccgatgacgc caccggatcc aacaccacgt
    18661 acgggtgggc gaagacgtac acgaacgtgt tccggacggg actgcccggg gagttcgcga
    18721 agtaccaggt caccttcgtc gggaccgtcg aggacttccg acgcgggata ccgtccgatg
    18781 gacccagtga gaactaccgc gacacatcga ggagccatcg ggacgagttg cgcgcacgga
    18841 tcgataccta caccgaacct cccgtcgtgt tcctgatcga ggagtactac ttgggcctct
    18901 gcaacgggct cgccgcggga gcctgttctc cggaaaccga gcgggccgcg ctcggtcgtg
    18961 ctctggagga ttcggtcgag atcgggcccg ggacgcacgt gttgaccggt gaccgcacct
    19021 acgagcctcc cgcggaggtc gtggagcggg cgcgttccgc tgccgatgcc gccgccgcgc
    19081 ggttcgggga tcctcccggg ccgctggccg atctgccgca tcaggcacgt gtcctcgccg
    19141 ggctgttcgc gctcgccgtc cttcccggac tgctggccgc ccccttcttc gggctgaagg
    19201 acaccgtcag ccggatcgcc ctgatcccgg gcacatcgat cgtgctgtcc ctcctctcgg
    19261 ggatcgccgt gctcggcgtg tggcgcggac cgctcaccga caccaaggca tgggtcgtcg
    19321 tgggagcggc ggtcggcgcg ggcgcggcgc tccggtacgg gagcgcatcg atcctccggg
    19381 cgctcggtgc gttcggcggg ttcttcaacc gattgttctc gcagttctcg aatgccgcct
    19441 acgcctctct gatgagcgtg cagttcctga cccaggccgg gcaagggatg atccaaggcg
    19501 cgatcggcaa gtcgatcgcg ttcggtggcc aggagggctt cgacgtgcag aacgtcccct
    19561 ctgccgacta cctgttgcag gtgatcctgc tcctctacat cccctacacc ctgctgtcgc
    19621 cgttcatcgg cgtcgtgatc gatcggttcg aacgacggcg ggtcgtgtgg tggtcgaacc
    19681 tcgtgacggc gggcgtggtg ggggtggtcg cgatcgcggt cctcctcccg ctcggcgagg
    19741 gcacgaccga gggagatgcg ggcgccaccg cggcgctcgt cctcgggttg ctcgccgccc
    19801 aggcggtgat ccgcgtggcg ctcgccgtga agtcggctgc gatccccgat gtactgtccg
    19861 gccgtgacct cctccagggg aacgccctgt cgcaggcggg cggcgcactc ttccagatcc
    19921 tcgggatcgc cgtggcgctc gccgccggcg ggttcctgtc cgcatggctc gtcgtgctcg
    19981 gaggcgccgc ggtcctcgcg atcggggctc tggcggccac ccggctacga cacgtggagg
    20041 cacgcgcaca cgtgacccgt ttcgccgagg aggcccgccg ggtactccgc acgatcgcgc
    20101 gcggggtccg ggaggtggca gctcgccccg cggccgcggt gggactgtcc gggttccaga
    20161 tgctccgcta ccagttctgg ggcttcgggc tcttcgtgtt cgcgctctac gcgaagaacc
    20221 tcgtcgaggg gggcccggag agccaggtgc cgacgctcct ctcaggcggc gtcggcctga
    20281 tcggcggggt cctcggactg atcgtggcgc aggggctgaa ggaccgggtg gctccgatca
    20341 ggctcctgct ggtgtcgatg gcgctcctcg gtgcgggaac cctggtgttc gggggcctgg
    20401 tctccgtcgt cggattcgcg gcgatgctct tcatcggctt cttcgcgttc ttcctgggga
    20461 aggtttcggc cgacacgatc gtccagcagg cgatgccgga cgacttccgc ggccgagcgt
    20521 tcgcattgtt cgacatcgcg tacaacctcg gcttcatcgt gccggctctg gtcctgtccc
    20581 tcgtgtgggt ggagaacgac gccggccgca cacgggtggt actgatgaca tcgggcgcgg
    20641 tgttcctgct cctcacgggc gtcgtcgcgg cgtgggcccg acggatccga gatcagttcg
    20701 cgacgcagga cgacctgatc gaggcctgac aggctgagga gccatgccgg aggtctgcaa
    20761 cggggacgtg acgctgcacg tcgagatcga tggcgccggc gagccggtga cgatcttcgc
    20821 gcatggcctg acgaactcgt gtcgcgagct ggcgccgttc accccgtcgc tcgccggtac
    20881 cgccgtgcgg ttctgcttcc ggggtcacgg ccattcgagc gcgccgcggc aggggtaccg
    20941 gttcgccgat ctcgcgtcgg acctcgacgt ggtggctcgg acatacgggg cgacgaacgc
    21001 ggtcgggacc tccctcggcg ccggggcgat catgtcgctc ctcgaacacg acccggcccg
    21061 cttcgaccgc atcgtgatgc tgctgcccgc gtcgctcgac cttcccttcc gcaacccgga
    21121 gcggttcgat gcgatcgcgg atctcctcga ggcgtatccg aaggacgagg cgatcgagcg
    21181 ggtcctcgag atgaacgagg agcgatacgc gcgggcgccg tggctccgcc agctcgacct
    21241 catgctctgg gaggacatga accccgccgg cgtgtcgcgg gcgatccgcg aggtcacgcg
    21301 cgacatctcg atctcggacc gcgagctgct ccggaaggtc gaggcgcctg tcctcctgat
    21361 ctgccgggag ggtgattcta tccatccggc cgccctcggc cggatcctgg ccgatctgct
    21421 gcccgacgcc gagctgatca ccttcaactc ggaggaggag ctgatcgcct cgatcccatc
    21481 actggtcgag cgcgtccggg cgttcctgga gggggtgtga gcgagagcga ggcgcacgac
    21541 cgccgcgcca ggacgcctcg ccaggagctg ctcgggtcca cgctctccgc gctcatggcg
    21601 ctgcagttcg cggcggtcgt gatgttcggc aaggaggtcg cggccggcga cctgcccttc
    21661 ccgatgctgg cgatccgatt cgcgggccag gcgatgctgc tcttcgtcgt tctgctcctg
    21721 tggcgcaaac cgctggcgcc ggcgccgggg gagcgcctgc cgctggtcct cgccgcgacg
    21781 atcggctacg ggaccgaggc ggccttgttc ttctccgccc tcaaccacgg gaaggcgggc
    21841 gcggtcacgc tcctgttcta cctgtacccg gtgtgggtga tgctggtgac gatcgggctg
    21901 gaccgccgtc ctccgggatg gcggctgttc gtcgccctgc tcctggcgct cggcggcagc
    21961 gcggtcgtga tcgtcggcgg cggtgaggtg gaggtcgcgc ccctcggcat cgtcctcgcg
    22021 ctcgccacct cggtcgccta cacggcctac ctgatcaccg ccgatcgaac cgttcgccgg
    22081 acggacccgg tcacggccgc cgcgtggctc ggggccgggg ccgcttgctc gaacgtcgtg
    22141 ttctcgctcg cgttcggcgt caaggatctc ccggacgcgt cgtcgtggcc gttcctcctc
    22201 gggatggcgg tgttctccgc cggcgccttc gcggccatgc tcggcggcct tcagctcctc
    22261 ggcgccgtgc gcaacgcgat catcggcgtg atggagccgc tgggggtcgc cgtcctcgcg
    22321 tcgttcttcc tcgacgagcc catcacggct cccaccgcta tcggcggggt catgatcctc
    22381 ggcggcgcga tcctcgccac gctcgttcgg accacccgcg tccgcgagcc cgacgtctag
    22441 tagcgtcgga gcatgaccga ccagctcttc gccgaggacg cctacctgac cagctgtgag
    22501 gccaccgtgg tcgacatccg cgacgaaggg gtgatcctcg atcgaacggt gttctacgcg
    22561 cgcgggggcg gccaacccgg cgacaccggg acgctccggt gggagaacgg gacggcacgg
    22621 gtcaccgaca cggtgaagat gggaggcgac atcgtccatc aagtggaggg cgacgcgccg
    22681 ctcgtgggtc attccgtcac ggccgagatc gactgggccc gccgtcacct gctgatgcgc
    22741 acgcatacgg cgctgcacgc cctctccggc gtgatcttcc gcgagcacgg ggccaaggtg
    22801 accggcggga acatggacga caggggatcg gcccgtatgg acttcgagct cgacgagatg
    22861 agccaggagc tcggcaagga tgtcgagggc aagctcaacg aggagctcaa caggaaccgg
    22921 cccgtgcacg tgagcttcct gcccagggcc gaggcgctct ccgatccgga tctgatccgc
    22981 acgaaggtca gcctgatccc cgacaccgtc gacccgatcc gcgtgatcga catcgagggg
    23041 atcgacaagc aggcggacgg cgggacgcac gtgaaagcca cgggggaagt cggtcacgtc
    23101 accgtcgtca agacggaatc gaagggcaag gccttcaagc ggatgcggat ccagctcgag
    23161 gcgtgacact catccgccga cgagcaaacc gatcacgagg aagacgaggt acgcgatgac
    23221 cccacccgcc gtgagcatca ggcgcagctg cacggggatc gaggatctgc cgaccgcgaa
    23281 gtagagcaac ggccccgcga acgggacgag gagcacgatc gccatccatc tcgcacgttc
    23341 cgggatcgca tcggcgtcac gcctgatcag gtcccacatc gcgatcgcca cccacgacgc
    23401 gtagagcacg aacggcaaga tgtagccgta gagcccgagg agcaggctcc accagctgga
    23461 cgcgatgagc gggactccca tgaggctgac ctcctggctg atctctgacc cgcgcgctgc
    23521 gcggacatcc cccagagcgc ggatccggta gcgcgccggc actgcctcct cggggatcgc
    23581 cgggtcggga ggggccgccg ccggatcacc cggcatgtcc gggaggcccg ccgggggatc
    23641 cggctgcgcc cgccagtccc cgtgcgggaa ctcgccgtcg agcgaatcga tgaaccggga
    23701 caggacgttc gcggttggtc ccatgctccc gcctccgccg ggaaagggtc ggaacccgct
    23761 gcaggggaag aggagctcca cggctgcggg gtggctcgtg tcgacgtcgt tgccgctgaa
    23821 gcagtcggcg cccgccgagg gtgccgcgag cgccaggtcc gcccggccgc tcctgcgaac
    23881 gacgttgtct cggacctcgt tgccgcccga gggccagaag ttcgtgtcca cgatcgggat
    23941 catcgcgatg ccgtacgtcg cctggtcctc gacgaggttc tcgacgatcc ggtcgtcacg
    24001 gccgccggcg accacgatcc ccatcccgaa cggcacgtac tcgtactcct tcgcgtccac
    24061 ggtggtcgag ttgttgtggt ggacgtggtt gccggcgatc aggacgtcgc gttggggcgc
    24121 gtacggctcc gagtcgagcg tgttcggcac gatccccgcg aggttctccc gccactcgct
    24181 gttcacgatc gcgaggttcc ctcccgcgtt ggtccccgag tagccgagcg cgttgtgctc
    24241 ggcgagcacg tcggtgatca cggcgtcaca cgggttgcac gcgccgatgt agaacccggc
    24301 gtcgggcgag ccgctcgcgt acgagtgatc gaaccgtccc cagcgcgacc cgaacgcgta
    24361 gatcccgtag tcgccgttcg ttgacgctgt gaggtaggag cctcggtacc ccagcacgtc
    24421 cgaccagtag aagccgttca acaggaagtg ccgcgcggtg aggttctcga cggcgacacc
    24481 gtccgcctcg atcacgtgga tcccgttcgc gagctcgaag ttcccctcga ggatcgtgtc
    24541 gttgcggtcc acgccgcgga tcgtgaggta cggggtggtg acgaccacgg cctcccggta
    24601 cacgccgggg tcgatcagga cgaggccgcc ggggcgggcg cggtcgaccg cgttttggat
    24661 cgtcggggcg tcgtcgggga cacgcacggt gttggcaggt ccgacgggag ccgggtcggg
    24721 acccggggct ccgccgccgg ggagcggcgc gtcgccgacg acgacggtgc cggccatccc
    24781 gaggccgggt cggccgtggt accggcagaa gaacgggaag atcccctcgg tgtcgaacgt
    24841 gtggtcgaac tccgccccgg gatcgagatc ctccgacgac cacgagccgt catccgcctc
    24901 gacggtgtgc ggagagcggc cgtccatcgt ccactcgacc gtctcgccga ggtcgatccg
    24961 gacgatctcc ggtgtgaaca ggttgtcgat cgccgagacc gacccgccgt acgatgcctc
    25021 gtcggcggga tcctgtgcct ccgcgggccc gggcatcatc aggaggagcg cggcgagcgc
    25081 cggggcggtc gcgaggcgac gcatcgcgcc ggaccctagc atgggcggat gcccgcggag
    25141 cgattgtcgg ggctcgacgc gagcttcctc tacctcgaga aacccggcgt gcacatgcac
    25201 gtcgcgggac tctcgatctt cggggcgcgc gcggacggct cgctcctcac ctgcgacgag
    25261 gtggagcggg tcgtggaggc tcggcttcgc ctggctccgc ggctgcgtcg gaagctgcag
    25321 tcggtcccgg gcaacctctc ccggcccgtg tggatcgacg acgaccggtt cgacctcgac
    25381 ttccacctgc gccgggcatc catccccgcc ccgggcggac ggttcgagct cgagcgggcc
    25441 gtgggtcgcg tgctgtcccg cccgctcgat cgaacgaagc cgctgtggga gctctacgtg
    25501 ttcgaggacc tctcggagga tcggacggcg atcctgctga agatccatca cgccatggcg
    25561 gacgggatcg ggaacatgat gatcgcgtcg gcactgttcg atctgaccgc cgacgcgccg
    25621 actggcgagc cggacggggc gtggtcagcc gagccgccgc cgccgatgca ggatctgatc
    25681 cgcgacgcgg tcgaggacgc ggtcctgcac ccgatccaga gcctcgccca cgtcgcacgc
    25741 cagccgcgca tcgttgccga aaccgtgtcc gcgacggcgg acgcgctccg cacggtcgcg
    25801 gggatgggga cggcgcctcg cgggccgttc gatggaaggg tcggtcccaa ccgccgcttc
    25861 gcgacggccg agcgaccgtt cgcggtgttc cgaggggtca aggagtcgct cggcggcacc
    25921 gtgaacgacg tcgtcctcgc ggtcgtcgcg ggaggcgtcc acgcactgct cgagagcagg
    25981 ggcgagacca cgcggggccg gtcgctccgg gcgatggtgc ccgtgtcggt acgctcgccg
    26041 ggcgacggcg gcgacgtcgg taatcgcgtg gctcccatct tcgtggacgt accggtgggg
    26101 cggatggcgg cgcggacgcg cctccggcgc gtccgcg
//
LOCUS       DASUYD010000125        37935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_66844, whole genome shotgun sequence.
ACCESSION   DASUYD010000125 DASUYD010000000
VERSION     DASUYD010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 37935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37935
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66844"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VF195_06790"
     CDS             <1..258
                     /locus_tag="VF195_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX6580559.1"
                     /translation="ADIDLIEINEAFAAQVLACERELKFDRDRLNLLGGGISLGHPIG
                     MSGARILLSLAYQMRTRGVSIGLATLCISGGQGLAVVLERT"
     gene            263..550
                     /locus_tag="VF195_06795"
     CDS             263..550
                     /locus_tag="VF195_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018837650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEX6580560.1"
                     /translation="MTVHAVVLIQCQIDEIPEAAEAIAEIGGVSEVYSVAGEFDLVAV
                     VRVAVHDDLETVIPGGISKVGGVERTETLIAFQVYSKHDLERMFSIGFEER"
     gene            complement(547..900)
                     /locus_tag="VF195_06800"
     CDS             complement(547..900)
                     /locus_tag="VF195_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6580561.1"
                     /translation="MTDGRPLVDLDYCYLTTTGRHSGEPHRIEIWFALAEDVVYLLSG
                     GGDRSDWVRNLEVSPDVVLEIGDQERATKARVVTDPEENALARRLVAEKYRPRSREDL
                     DDWERTSLPVAIEWG"
     gene            complement(1007..1237)
                     /locus_tag="VF195_06805"
     CDS             complement(1007..1237)
                     /locus_tag="VF195_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580562.1"
                     /translation="MVAVAADGNGFAVEVRGGEETWTVAIVSPEGDVVSERACHDGAE
                     ARTYASTVRQHIFWLSPEKFREYYRIQGQVEG"
     gene            complement(1240..1527)
                     /locus_tag="VF195_06810"
     CDS             complement(1240..1527)
                     /locus_tag="VF195_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580563.1"
                     /translation="MKIKGTCRRDGREFFAEQVVASGGECPWDGQPFNADYAAVLVES
                     LREAERLGTELERALERVASLHPAFTLDEHSVLGELTDQLGRFHRNLVRQA"
     gene            complement(1524..1964)
                     /locus_tag="VF195_06815"
     CDS             complement(1524..1964)
                     /locus_tag="VF195_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010839043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580564.1"
                     /translation="MDVLLVSPDERSRQLMALAVRSIERRLGEQIRFRAARNGELGTW
                     AALRERPDMIVADEIASREGAFGLARTLRDHAEPYAGVIVILLDRRHDAWLAKWSGAD
                     AWFVKPVDPFELADRLLELVTESKTKTEPENKPENKPENEKESA"
     gene            2141..2830
                     /locus_tag="VF195_06820"
     CDS             2141..2830
                     /locus_tag="VF195_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007906767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEX6580565.1"
                     /translation="MESLGKAAVAEFVATFALIFVGAGAVIVASSGQLDLVGVALAHG
                     LVLAVMVSVTGHVSGGLVNPAVTIALWAAGKMGTQRGAVLIVAQLVGAVMGALLLRYV
                     VGGAAFDGGGAGAPALAPGLAVGKGIVLEAMLTFFLVFVVFGTAVDDKGPWNKTAGFT
                     IGLTIAFDILAFGPLTGAAMNPARWFGPALVGGIWDDALVWIVGPIAGAIIAGVLYTA
                     VLLRGREPATP"
     gene            2862..4046
                     /locus_tag="VF195_06825"
     CDS             2862..4046
                     /locus_tag="VF195_06825"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEX6580566.1"
                     /translation="MVADLYLDSASATPLLPEARDAVVSSLESYGDPLSIHALGRAAR
                     SLLDDGREQVATAIGAQPDEIVFTSGGTESIALAIWGGVRAVRELGTRIVIGAVEHPA
                     VSGVAHALESDGFEVVAVPVDRDGRVDLDRFAAEVRTPGTLLASVQHANHELGTIQPV
                     AEAARLAREAGVRFHTDACQTVGRLPVDGPALGVDLLSLSAHKFGGPPGAGALYVRRG
                     VAITAYPCGDDRERKRRSGMENTPGIAGMAAALTASLTTMADRAAAHWTLTAELREQL
                     AEVPGTTVHGHATHRTPHLVGFSVAGLDPATLLMTLDDRGFRLGAGSLCSGRPEDPSP
                     VLEQIGYPGTTGFRVSLGDGADRSDLDALVGMISGTVRELRQMEVASTEALSRFSPPS
                     AG"
     gene            complement(4082..5125)
                     /locus_tag="VF195_06830"
     CDS             complement(4082..5125)
                     /locus_tag="VF195_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009327591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX6580567.1"
                     /translation="MSDVIENGGTAPGDASTLTKKDFTSDQEPRWCPGCGDYAILSAV
                     QAFMPELGIEPHKTVFVSGIGCSGRFTYYMDTYGMHGIHGRAPALATGIAVAAPELSV
                     WVVTGDGDGLSIGGNHLIHALRRNVDIKILLFNNQIYGLTKGQYSPTSEEGKVTKSTP
                     FGSVDHPFNPIALALGVEATFVARTVDNDRQHLTEVLRRAAQHKGTAFVEIYQNCNVF
                     NDGAFDLLRDKAQGKHNQIRLEHGEPIVFDEGNRCVRVGENGRYEIAPTAETDPDSVV
                     VHDAHGRPSLAFALAHLSRGPDEPSCIGVFHEVERPVYGQAIQHQLQAAKERLGAGDL
                     GSLLHVGDTWTVE"
     gene            complement(5118..6953)
                     /locus_tag="VF195_06835"
     CDS             complement(5118..6953)
                     /locus_tag="VF195_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019547129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX6580568.1"
                     /translation="MSKAVRERERIVVRFAGDSGDGMQLTGDRFTSATAVLGNDLATL
                     PDFPAEIRAPAGTVHGVSAFQIQFASNDITTPGDHADVLVAMNPAALKADLDKVGKGG
                     VLILNEDSFTERNVQKAGYTADPLQDGTLDGYQVYKVPMTSITVRATEGIGIGKKESE
                     RAKNMFALGLVSWMFGRPTDTTLNWLEKKFGSRQEIFDANVAAFKAGYNFGETTELFA
                     ESYKVEAAPQVPGTYRNIDGATALAWGLIAASERSGLPLFYASYPITPASELLHELSR
                     HKNFGVVTIQAEDEIAAANIALGAAFSGQLAVTGTSGPGIDLKSETIGLAVIMELPMI
                     IVDVQRAGPSTGMPTKTEQADLLLAMYGRHGESPLPIVSASTPAECFTAALEAARIAV
                     RYRTPVILLSDTFIANSSEPWPIPDVESLPSIDPGFASPNGQDFLPYSRDERLARPWA
                     IPGTPGLVHRIGGLERQDGTGNISYDAANHELMTRLRQAKVDGIADDIPALEVEDPSG
                     EAELLVLGWGSSLGTITAAVARARADGRSVATAHLRHLNPFPTNTGEVVRAYRKVLIP
                     EMNMGQLAALIRARYLVDAASYSKVQGLPIFAEELEHEIARVLDE"
     gene            7016..7936
                     /locus_tag="VF195_06840"
     CDS             7016..7936
                     /locus_tag="VF195_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6580569.1"
                     /translation="MRRRQLGRHGPEISVIGYGAWEAGGTAWGANESEEVVIDAIRAG
                     LDAGIDWIDTAEVYGDGVSELLVGRAIAGRREELTLATKVAPAPEGSGFTPAQVRSAC
                     EKSLGRLGTDRIDLYQLHWPDETGVPLEETWGAMGDLQDAGLVRHIGVSNFDRGQIEA
                     CIAMRHVDSLQPEFSMLARANGDLIRWCGEQGVGVVSYAPLAYGLLTGAITAETRFQE
                     GDWRGAEQDEGPFSDVEASLDIVGRLRPVAERLGCGLAELALAWNVHQPGVTSAIAGS
                     RNPEHVRTNAAAGDIELDDVTRKELDTLLE"
     gene            complement(7937..8344)
                     /locus_tag="VF195_06845"
     CDS             complement(7937..8344)
                     /locus_tag="VF195_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580570.1"
                     /translation="MAALNAVPLFDGFSKRHLLRLVAEAEVVAFEADRVIVEEGGAGE
                     AMFVVLSGGARVSRGGKKVATLVPGDFFGELSALDGGPRTASIVSETPMELLRLFRHT
                     LRRMIEEEPSLAMGLLEGLARRLRQVQPGGPVT"
     gene            8504..12133
                     /gene="metH"
                     /locus_tag="VF195_06850"
     CDS             8504..12133
                     /gene="metH"
                     /locus_tag="VF195_06850"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX6580571.1"
                     /translation="MDVQSRLRALLAERILVLEGPKGTAIQALELSEEDFRGDLLKDH
                     PRDIRGDNDVLGLTRPDIVESIHRDYLDAGAWILSTNTFNGSPVSQAEYGLEHLVYDM
                     NRAAAENARRALDGAEDRFVVGSLGPTNRTLSISPKVEDPAFRTITFDELTEAYAEQI
                     RGLADGGVDVLQIETIFDTLNAKAAIAAAQDVAPDLPLWLSFTAVDQSGRNLSGQTAE
                     AFWLSIEHAEPMIVGVNCSLGAAQMRPYLEALSRIAPTYTACYPNAGLPNAMGLHDED
                     PEITSAYLGEFARAGLANVLGGCCGTTPEHIRQIAEAIRDLPPRTVPDPDETFTSFSG
                     LEPFVVRPDTGFVMVGERQNITGSKKFRRLIESGDFQAAVDIAREQVQNGANLLDVNM
                     DTDLLDGPQAMTTYLNLLATEPEVARVPIMVDSSSWDVIEAGLKCVQGKGVVNSISLK
                     EGEDDFLDKARRVQRYGAAVVVMAFDEQGQADTVDRKVEISERAYRLLTEHAGYDPSD
                     IIFDPNILAIATGMEEHADYAKSFIDATRIIKRRCPGVKISGGVSNLSFAFRGNDVVR
                     EAMHSAFLYHARQAGLDMAIVNAGQLPVYDDIQKDLLEHVEDLLFNRRPDATERMLTF
                     AQTVTGEAMRTEQDLTWREGSVEERLSHALVHGIDAFIEEDAEEARQGYERPLEVIEG
                     PLMDGMQIVGDLFGAGKMFLPQVVKSARAMKKAVAYLEPFMQEEKERLGLVGSAQGTI
                     VTATVKGDVHDIGKNIVGVVLGCNNYRVVDLGVMAPAETILDTAAREKADVIGLSGLI
                     TPSLNEMVNVATEMQRRDLDLPLLIGGATTSKQHTAVKIAPVYAQPVVHVVDASRVVG
                     VMSALLDEPRKAKLDAENRTDQERLRTLHEEKQKQPLLPYRKALENRLKVDWRQEDAP
                     PPPFVGTRTVEPDLATLCGYIDWTFFFTAWELKGSYPQILDHPQMGETARELFQVGQE
                     VLDRIVDERLLEARGVYGYFPARAEGDDIVTEGGYVFHCLRQQGDRGEGEPNKSIADL
                     VAPLDSGVRDHVGAFAVSAGFGADDLAKAYEAEHDDYRAIVTKALADRLAEAFAEYLH
                     QQVRREWYAPDEQLSSEDLIKERYRGTRPAFGYPACPDHSEKRTLFELLNAAEETGID
                     LTTSCAMMPPASVSGLYLAHPAARYFHVGRIAKDQVEDYARRKGESLAEAERWLAPNL
                     AYEPG"
     gene            12188..13438
                     /locus_tag="VF195_06855"
     CDS             12188..13438
                     /locus_tag="VF195_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010147541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6580572.1"
                     /translation="MQSYWHPFADMAAIAAGGELVLDHGDGCYVWDVDGTRYLDATAS
                     LWYCNVGWGRAEIGEAAAAQMAKLPTYSNFGDIVTPVTLELADRVAGLAPVPGSKVFL
                     TSGGSDSIDTATKMARRYWQLLDEPDRTILVRREKAYHGMHTAGTSLAGIPANAAGHG
                     ALIQDVLEVPWDDPDSLRAAIDDVGPEHVAAFFCEPVIGAGGVFPPPDGYLEAVRAVC
                     RDTGVLFVADEVITGFGRCGDWFASTRFGLEPDLVVCAKGISSGYLPLGAVLASWGVA
                     EPFWAEGAGMWRHGYTYSGHAAVAAAALVNIDILEHEGLPARAKTMEGALLDALKQLT
                     DHDLVEDVRGGVGLLAAVQLRSDLVDQDPALGARVGAATRDAGVLVRPLVGGALAVSP
                     PLIIEGSQLQELVDGVRAGLDAVA"
     gene            13480..14166
                     /locus_tag="VF195_06860"
     CDS             13480..14166
                     /locus_tag="VF195_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580573.1"
                     /translation="MDTIVRPTTTHSEPPPIRHRWVRLLILVSAFTGALAITLGTMWV
                     TNYMPLVQGSVAFGTARTDVRAVQVDAFDMRGTVFTVPTGRNATFHYRFSLSNEGPVA
                     VRIESVGTPSREGPLTRRAVEVIPDPWGSEGPIGYTSEEPWHPFTLSPGGEAVVVMEA
                     RYDGRCLDRGDGAAWYWEPLRSPSSGYRGRTSWRSASKSDSAATGVARAASGTSARRR
                     TSSAPPDCRS"
     gene            complement(14108..14473)
                     /gene="erpA"
                     /locus_tag="VF195_06865"
     CDS             complement(14108..14473)
                     /gene="erpA"
                     /locus_tag="VF195_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX6580574.1"
                     /translation="MAQTAEETAPETSRTSIQLTETAAGEVQKLITEEGRPDIALRIA
                     VQPGGCSGLRYAMYLDDQVSEKDLLEEQFGVKVVVDKMSVPYLTQATIDWVDSLEASG
                     FTIDNPAAQSSCACGHSFH"
     gene            complement(14521..14988)
                     /locus_tag="VF195_06870"
     CDS             complement(14521..14988)
                     /locus_tag="VF195_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009083020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6580575.1"
                     /translation="MGAIERFTDAARELGLEPEIRRFPEGTKTAVDAARAIGCDVAQI
                     VKSLVFMADGNPVIAYTSGGNRVDPSKLAGLAGASESRRATPDEARAATGFAVGGTPP
                     FGHPAPLRSFVDPALLTFQEIWAAAGTPDSVFALTPSQLRRATGAETADFTEG"
     gene            15061..15891
                     /locus_tag="VF195_06875"
     CDS             15061..15891
                     /locus_tag="VF195_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HEX6580576.1"
                     /translation="MSLDRTTLLGIAQAERQRLGRTVQFADPQTWEQPSALPGWWNRD
                     VMAHLAAQDTAAAHVVKGDPPEELEEYRRHLDGQPFTVDGFNEWAVERRSGAPTREIL
                     VTWGQAADALLGYAGLLSEDEWDKGRFPWLAGEIAARYLIQSRIVEWWVHGEDMRATN
                     GLGPEYQHWPIHLTIDMGIRMLPWALAEAGLDLTGYSVEVEVDGAGGGRWHWGLGAGE
                     VPPADTKPDTTIVGRAPHLALVAARRIPAEDVLASGNLVTGIDAQLGVTILRHIRAFV
                     "
     gene            15958..16551
                     /locus_tag="VF195_06880"
     CDS             15958..16551
                     /locus_tag="VF195_06880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580577.1"
                     /translation="MPPDERDADQRLMRSFAARDAGAAEVLYQRFAGRIYGLGLVMLG
                     NEASAQDLVQDTFVKLWRNADRYDRSRGKLETWVLLMARSLAIDAIRRRVLEVRTLES
                     VDRPTEADQGPGPDERAVTVDLTDRARRAMSSLPAEQRAALELAYLGGKTSAEISDLE
                     GIPVGTAKTRIRAALLRLRDLLASDIESARGGDRDDL"
     gene            16538..17257
                     /locus_tag="VF195_06885"
     CDS             16538..17257
                     /locus_tag="VF195_06885"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX6580578.1"
                     /translation="MTTCEDVRPHLPDLLLGAAPDPDELDVRWHLRGCGACRRELDAL
                     EEGLHVFGSTLERPAPSGLHDRVITVLSEEWAGETTTRGRVPAPRGWRIAVAASISLA
                     VLAATFGLIQLGKAREEARDAASFRTVLATLGGTGFKVGTLQPATQTPVAGSVVAYES
                     SHDQSFVVVFVRTPHLMGRGSLLVSSSDGTTWNPGPIEFEGDGDAAAWWVTDRSIDSM
                     SGLTIRSPDGSTLATAGLRPA"
     gene            complement(17254..18249)
                     /locus_tag="VF195_06890"
     CDS             complement(17254..18249)
                     /locus_tag="VF195_06890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580579.1"
                     /translation="MTLDVVFSEDPRDFLRRDWSELVRVDPAGTVFHTPDFLKLYWEE
                     FGEEPDHLLLAFGEDAGTQVAAAAFERINDTLRFLGGTEVTDYLGPVGRPEVATAFSD
                     RLWAELLDRADWRTADLRGLPEGSAWLPALRDAAVVHGLAVEETEDQNGVAPFLELPT
                     TWDAYLQQIPSKLRHEIRRKAKKLEAEAGPFQIETATSGTLLPLLDRFVELHRMSEGP
                     KGVFMVPGMEIFFRRLGQALCERGTFRLSFIRVNGELAAGTIGFVFEGTYSLYNSAFD
                     RGWQRLGPGMVLVAEDVREAIEHGCAVFDFLKGDYAYKYRFGARRRSVNRLVVSR"
     gene            complement(18246..20066)
                     /locus_tag="VF195_06895"
     CDS             complement(18246..20066)
                     /locus_tag="VF195_06895"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEX6580580.1"
                     /translation="MPTTPRRTRSLKLPTFAAAALLLSLGGSAAAQTVGEGIHSYMVE
                     IRILGDGDLAVTETIDYDFGSGLRHGIFRTIPTRFHHDDVYDRVYPIRNVTVESPTAP
                     SDVDVSEEGGSTVIRVGDPDVEISGRHTYTVSYRVEGALNAFDEHDELYWNAIGDEWQ
                     APIESVLVVVRAPAEVQRVACFQGWEGGTDVCGRATHDGRTARFSAGRTLLAYEGLTI
                     VVAIPKGAVPEPSAILEERWSAGRAFSLDGTKIAAAIAVTGLLGGLLGRLWWREGRDR
                     RFIGSQIDQVMGSPSGESERVPPGEGDAEAPVEFAPPENVRPGQIGTLLDERANVIDV
                     TATIVDLAGRGFLLIQEIPDTGLFSKTDWTLIRLEGEESELLAYERKLRDGLFRDGNQ
                     VKVSELKTTFSDRLHGVESALYSDAMRQRWFRARPDQVRLRWAGAGVLLLVAGAAVTF
                     VLARWTHWGIVGVPVIVAGLALALMARQMPARTAKGTAMLRRIRGFRRVIATAEQHMA
                     RWAEEENVFTRYLPYAIVFGLTKKWAKAFEDLGLQPDTSGWYVGPRMLTAAAFADSID
                     GFAVTTGGTLASTPASSGSSGFGGGGSSGGGGGGGGGGSW"
     gene            complement(20102..20551)
                     /locus_tag="VF195_06900"
     CDS             complement(20102..20551)
                     /locus_tag="VF195_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018930855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX6580581.1"
                     /translation="MALDDIYKETILDHYKNPRNKRELPGAVVTCTKNNPLCGDEITV
                     SANLEDGKLLEVTFEGAGCSISQSSASMMTESVGGKTVEDAMSLAADVRGMLAGEVEP
                     DEDVFGDLVALKGVVKYPIRIKCAVLAWDVLQEAVSDDGAASGAAAH"
     gene            complement(20569..21825)
                     /locus_tag="VF195_06905"
     CDS             complement(20569..21825)
                     /locus_tag="VF195_06905"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEX6580582.1"
                     /translation="MSAKEQLGTATSVLDVERIRRDFPILERTINGHPLVYLDSAATS
                     QKPRQVIDAVSDFYANHNANAHRGIYRLGEESTEAFEGARAKLAAFFGSPDPRTIVFT
                     RGTTESLNLVAQGWGRKFLKEGDEVLLTDMEHHSNIVPWQLTAQVTGAVLRYIPLTDD
                     GQLDLSELGSLLNERTKMLSVTGMSNALGTITPLGQLVEAAHAVGALVAVDAAQLVPH
                     NGVSVGELDCDFLAFSGHKMLGPTASGGLYAKAELLDAMDPMFGGGEMIHEVFHDHST
                     WKDIPYKFEAGTMQIAQQVGLGVAVDYLQALGMDAVRAHEEEITRYALDTLLSAGATV
                     YGPKDAAARGGAVSFWFRDVHPHDLATVLDEQGVAIRAGHHCAQLVMRRFETPATARA
                     SFYVYNTRDEVDVLVDALAKAEAFFR"
     gene            complement(21822..22610)
                     /gene="sufC"
                     /locus_tag="VF195_06910"
     CDS             complement(21822..22610)
                     /gene="sufC"
                     /locus_tag="VF195_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014644162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEX6580583.1"
                     /translation="MSENNGRAMLEIRDLRAEVEGKEILKGIDLTVTQGETHALMGPN
                     GSGKSTLSNIIMGRPGYVLTGGDVIFKGEKITGLTTDQRAKRGLFLALQYPTEVPGVS
                     VVNFLRTAYQAVKGEQMSALAFRKHMKEQMDRLGIEDAMVQRYVNQGFSGGEKKRNEV
                     LQLAVLQPEIAILDETDSGLDIDSLKLVAGGVAELVGPDLGVLLITHYQRILNYIAAD
                     RVHVMMQGRIVRSGGPELASELEEKGYEGIREELGIEDGGEEKG"
     gene            complement(22615..22932)
                     /locus_tag="VF195_06915"
     CDS             complement(22615..22932)
                     /locus_tag="VF195_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013017698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HEX6580584.1"
                     /translation="MGRVKVCDVAEAPAPGEAKRFPVDGRLVALVNLGDEGLRAIDAI
                     CSHAHYFLDEGDVDADFETIECPKHGSSFDLNSGKPRSLPATTPVDVFPITTEDDDIY
                     IEV"
     gene            complement(22932..24260)
                     /gene="sufD"
                     /locus_tag="VF195_06920"
     CDS             complement(22932..24260)
                     /gene="sufD"
                     /locus_tag="VF195_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEX6580585.1"
                     /translation="MAAVSTTTFDERALQSLPRAPGFVEQLRADALQRFEAMPVPSQE
                     TEEWRYTDLSAFELDFLPLADGGGAEDLNRHGLLGSAGVTGERAGLQIQRNSTVLSTQ
                     RSTALTDRGVWFGSLDEASETHPDLVEPFLHQLVPAGRTKFTALHAAFRTGGTFLHVP
                     RDTALELPIQTLTYLDADGAAVFPHTLVVVDAGSEVTFIDRYASPDLRRAFSDAITEI
                     HVGDGAHVRYVSIQEWGSGVTHLGIQRATVGRDATLRTLNVGFGASLARAEAETILAE
                     RGGFSEMLGIFFADEDQHFDHRSIQDHVAPNCTSDLLYKGALRDHSRAVYSGWVHVRP
                     DAQKTNAMQTSRNIVLSEHAKADAIPNLEIEANDVRCGHAASVGPVDEDAIFYLTSRG
                     IPRAEAERLIVSGFFQEVLDRVKIDEVREGAEQAIQDELDRAALGQGDPN"
     gene            24407..26980
                     /locus_tag="VF195_06925"
     CDS             24407..26980
                     /locus_tag="VF195_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580586.1"
                     /translation="MTTTTQDSAGAALRAFMIADIRGYTRFTREHGDAAAARLAGRFA
                     ELARDAVEARGGEVLELRGDEALAVFGSTVPAVRAAIEFQALCAEEFGEDPTLPLAVG
                     IGIDIGQAVPVEGGFRGVALNMAARLCSNAAAGQVLVSRAVADSASDAEGIRFESRGD
                     TALKGFEGAVPLIEALDELPAAPPVPATATAPGDALAPELDPMTPLADRVREMRWLRG
                     TWRQARRGRGRVLWISGPSQIGKTRLTAELASMVHTAGGRVSYAGLGGTADAEALRAI
                     RGITDHAQPALLVFDDVDAMGDEVARELEERLDTIAVRPALVVATIRAPEVSPSIERV
                     LRRSDPSGDGLRRLAPLDADGVRQIAADYVDVAVDEVPLEAFVRASGGIPGRVHEVVG
                     EWAREEAGRRLAAAAEWLSEGRARRNADIAFANNVIGLKLGRLYAPAEGDDRSVCPYK
                     GLATFEQGDAAFFFGRERLVGDLAARTVGAGLLGVVGASGSGKSSLVFAGLLPSLRAG
                     LLPGSERWRAVTMRPGEHPLAELEVALAGTSTPGDPAEAVRSIPPEERLVLVVDQFEE
                     IFTTCEDEDERGRSVAAICGIARSAQDRVVVVLTIRGDYYGHCARYPELAELLAANHV
                     LVGSMTREELRRAIELPARRVGLRVESVLTDTLVEEIADQPGALPLLSTALVELWQES
                     GALAPPRDLRANGRRPRRRCPHRGQLVPSVVRRPAGGRARDLPPPRRGRRGRGDHQAA
                     GPPRRVRPRAGSGGGRGAHTAHPRSVAHDGRLDGRGVARGAPPGVAPPPGLARGRRDR
                     TPAASAPDICREAMGGLGGRRLGALPGSEALRRARLVLRARAGAERTRTRLPVGQPAS
                     Q"
     gene            complement(27612..28076)
                     /locus_tag="VF195_06930"
     CDS             complement(27612..28076)
                     /locus_tag="VF195_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580587.1"
                     /translation="MTDQILLAGSRRGIPEGDVSVLATGRQDRAVRREQHRLDGAVMG
                     AFHLHELAGVEVQDRDRAVPAAEREQRAVGSEGLACPPRTLDHDVAQERSGPHVSDPH
                     PLPVIEGEQPVPIQHPRALALDPDGRSRELAAPEGEETDRAPRDPDHLLAVG"
     gene            complement(28073..28312)
                     /locus_tag="VF195_06935"
     CDS             complement(28073..28312)
                     /locus_tag="VF195_06935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580588.1"
                     /translation="MSRNRTRKSVPIIASDRPSAVTENCGEEHPSGQGGPGRAKVRTI
                     ARSPVTSHAISVLSSPPLTNVRPPCVNAAARSPLL"
     gene            28406..29236
                     /locus_tag="VF195_06940"
     CDS             28406..29236
                     /locus_tag="VF195_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX6580589.1"
                     /translation="MFGLAQAGLPGPDAVSLWDVRTGKLLRHLFTSEEPWTDLQHAPP
                     LDTVAYAPDGASVATNESERIHVLDPQTGETLATFEAPAFVQWLSYSPDGRLLQAEMD
                     GGLVAVWDLRTERMVWSRKVDDQTPLGGEFSADGRYLILGSESGKIHLLDPRTGRPLR
                     DPLLAHDAYLASITVHPDSSMFASSGTDGLTFLWDTSTQRALGSPFGVAGGGGVWSRF
                     SPDGRVLYVSTAERGFVFDTTLDTWIERACSIAGRTLTRTEWERFLPQRPYRPACRTT
                     "
     gene            complement(29360..30772)
                     /gene="sufB"
                     /locus_tag="VF195_06945"
     CDS             complement(29360..30772)
                     /gene="sufB"
                     /locus_tag="VF195_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX6580590.1"
                     /translation="MAVSQEEHLKELSKGYRFDWKDPSHSVFEPKRGLSTQVVEEISE
                     LKSEPDWMRKYRLKALKHFESRPMPWWGADLSDIDFENIYYFIRSTEKQAQNWEDLPE
                     DIRGTWDKLGIPEAEKKYLGGVSAQYESEVVYHKIKKELDDIGVLFTDMDSALRDYPD
                     LVKEYFGTIIPPNDNKFAALNTAVWSGGSFIYVPPGVAVDIPLQAYFRINAENMGQFE
                     RTLIIADEGSYVHYVEGCSAPIYTSDSLHSAVVEIVAKKGARVRYTTIQNWSTNVYNL
                     VTKRAAAYEDSVMEWIDGNIGSKVTMKYPSIYLLGKGARGEVLSVAFAGKGMHTDAGG
                     KAIHAAPYTTSVINSKSVSKDGGRTGYRGLVRVEPDAHHAKSTVRCDALILDDESRSD
                     TYPYIEVEDETAALGHEATVSKVGDDQLFYLMSRGLSEAEATAMIVNGFIEPITRELP
                     MEYAVELNRLIELQMEGSVG"
     gene            complement(30847..32010)
                     /locus_tag="VF195_06950"
     CDS             complement(30847..32010)
                     /locus_tag="VF195_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014134236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX6580591.1"
                     /translation="MRLLELGAVIVGLAGLARIAARFDLPTVPLYLLAGLAFGEGGIL
                     PLVTTRGFVETGAEIGLILVLFVLGLEHSSRDLIATTKASAAPGVVDLALNFTPGLLI
                     GLILGWGPLAAAFLGGITYVSSSGVAAKLLEHAGPHARGASRFVVTIAVIEDLVMAIY
                     LPVLAALLIGDEALTSLAAAAVAILVVGLLLVVALRVEVGLSQMLFSRSDEALLLTIL
                     GFAILIAGVADVARFSAAVGALLAGIMLSGPAAEGAQALVRPLRDLFAAIFFAFIGLS
                     VDPSTIPPVLAMAVLLGIVTSGTKYATGWWAARHLGSEERARGVVGAALIPRGEFSIA
                     IAGLATAAAVDEDLGALTVAYVILTVVVGSVVTKVLARRVDRWDPADVAARHG"
     gene            32125..32793
                     /locus_tag="VF195_06955"
     CDS             32125..32793
                     /locus_tag="VF195_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014676149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017128 - phospholipid scramblase activity
                     [Evidence IEA];
                     GO_process: GO:0017121 - plasma membrane phospholipid
                     scrambling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid scramblase-related protein"
                     /protein_id="HEX6580592.1"
                     /translation="MDDERRVQGQVERAAATGAFVGGGTLFTEPVLVVNQKTKIIELT
                     NEYSVFDAAGMQLGAVRQTGQTTLKKALRLVSSIDQFLTHTLEITDAAGVTQLTLTRP
                     RKVFKSTIVVARPDGAEVGRIVQENMVGKIRFGIEGGGSRLGGIQGENWRAWNFGITD
                     AAGTEIGRITKTWEGLARTAFTTADHYVVQIHKELPEPLRSLVVASSLCIDTALKQDE
                     RGFN"
     gene            complement(32829..33671)
                     /locus_tag="VF195_06960"
     CDS             complement(32829..33671)
                     /locus_tag="VF195_06960"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017613065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEX6580593.1"
                     /translation="MTDYAKPDVLVSTDWLEEHLDDPAVRAIEVDEDTAAYEKGHVRG
                     AVAWNWTTDMHTDVGRDYRDRAGFSELLSAAGVGPNTMVVLYGGNNNWFAAYAYWLLK
                     LRGFDNVKLLDGGRKKWELESRELTQDAPSYQATGFSISGAERPEIRALRDEVLQKAG
                     STKFVDVRSPEEFRGEKIAPDHLPQEQAQVPGHITGAANIPWAKAANEDGTFKSADEL
                     RELYAAEGITGEGEVIAYCRIGERSSHTWFALQELLGFENVKNYDGSWTEYGSLVGAP
                     VELG"
     gene            complement(33952..34350)
                     /locus_tag="VF195_06965"
     CDS             complement(33952..34350)
                     /locus_tag="VF195_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011530414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEX6580594.1"
                     /translation="MSEPETSIPRTQEAPEASPEPASTGTVRTNGGAPSEEQVLEALK
                     SVIDPELGINIVDLGLVYEVDVAEDGDVDIQYTLTTMGCPIGPLIEDQMRAFLAPLDG
                     IGEVRPEMVLRPAWNPEMMSEEAKAALGMF"
     gene            34460..35101
                     /locus_tag="VF195_06970"
     CDS             34460..35101
                     /locus_tag="VF195_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580595.1"
                     /translation="MTGIRALIVDDHPVTREGLRTALSMSDDAVVVVGEAASGQEAID
                     QARDLSPDVVFMDVRMPGMDGIEATRRIREASPETRVILITIDESRGAISEAIQAGVS
                     GYLLKDASPDALVDAARNAVEGNAVIHPQLTKTFIEEVHLVEAEGRTTPLSKREREIL
                     QKVADGATTRQVASDLGISPHTVKTHLERIFEKLGANDRAQAVAIAIRTGIVH"
     gene            35147..36340
                     /locus_tag="VF195_06975"
     CDS             35147..36340
                     /locus_tag="VF195_06975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580596.1"
                     /translation="MPTTVLLCDTSDEVARFQYHLLREHDLEVEVTTDAFRGVEAAVR
                     SRPDVIVSELTLEGLGGAELVKRFLASSPSSRFVAHTVVRDPVRVTEVLAAGASGYVL
                     KEDPVEDSLRAIRAASTGAVTLSGQVAGLLATELARAVSRSRELETELVALRHDVAQG
                     STAKADFLANISHELRTPVTVAKGIGYVLKNPAVPDEERNEFLGQLTGSLDKLMGIVD
                     ELITLSELERGTFELNLVETDLAPLVRHAIDELRMRYPGIPIAAKVSDGLLCIADGPR
                     LGSVVRELLDNACRYSPFGRAVELVARDLAEGVTVMVTDRGEGLDRTVAARAFDQPFS
                     TGEGVLHKEKSGVGTGLHLARQLVVEHGGVMWVDPLPGGGTRASFCVPHRRGERVEAP
                     PAHVA"
     gene            36407..37102
                     /locus_tag="VF195_06980"
     CDS             36407..37102
                     /locus_tag="VF195_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580597.1"
                     /translation="MRRSLLGIGVVVVVSACTSASPTTSPLQELLVTPSPTAFDLVTA
                     GQVRGVVPDRWEATLVDPSSGIRGGFVASPRQGRWSDVDGRTVGMSATWVDATRVGVP
                     SDFYYLAANGPLLSRLTTSANCRGSRHVYLDRRPTFDLESHSPGHFMAEGAGVCHGGA
                     RATRFAYFVAAPGFGPVHRVGIPSSGLYVVVAVVNQRRGAKNLLEELMRRTRFGDDAI
                     QDFVAMAPPAQSA"
     gene            complement(37118..>37935)
                     /locus_tag="VF195_06985"
     CDS             complement(37118..>37935)
                     /locus_tag="VF195_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6580598.1"
                     /translation="VIEDLIVYRLGEERYFVVPNASNAPRVLRMLEEEKADEPLHLMY
                     HQDWCFLAVQGPEAMGAIAATFPTAPDLAFMRCMETEYHRRPVIVTRSGYTGEVGFEL
                     FTYQDVARQLWDELLGSVRPLGGAPCGLASRDILRLEMGYPLYGQDLFEASTAFEAGL
                     GWAVAMDKGDFRGRTALLRQQEEGLPSRLRGLRMHERRYIPRAHYPVFVGDQLVGEVT
                     SGTFSPTLGTGIALAYLWPGDVVELGQEVEVDIRGRRGAATVVQPPFVDRSPR"
BASE COUNT         5719 a        13244 c        13003 g         5969 t
ORIGIN      
        1 gccgacatcg acctgatcga gatcaacgag gcgttcgcgg cacaggtgct cgcgtgcgag
       61 cgtgagctga agttcgaccg ggaccggttg aacctcctcg gtggcggcat ctcgctcggc
      121 catccgatcg ggatgagcgg cgcccggatc ctgctctcgc tcgcgtacca gatgcgaacg
      181 cgtggggtct cgatcggcct ggccaccctc tgcatctccg gcgggcaggg gctcgccgtc
      241 gtcctcgaaa ggacctgagc cgatgaccgt ccacgccgtc gtcctgatcc agtgccagat
      301 cgacgagatc cccgaggccg ccgaggcgat cgcggagatc ggcggtgtct ccgaggtcta
      361 ctcggtggcg ggcgagttcg acctggtcgc ggtcgtccgg gtcgctgtgc acgacgacct
      421 cgagacggtg atcccgggtg ggatctccaa ggtcggaggc gtggagcgca ccgagacgtt
      481 gatcgcgttc caggtgtatt cgaagcatga cctggagcgc atgttctcga tcggcttcga
      541 ggagcgctag ccccactcga tcgcgacggg gagcgacgtc cgctcccagt cgtccagatc
      601 ctctcgggag cggggccggt acttctcggc cacgagccgc cgtgccaacg cgttctcctc
      661 gggatccgtc accacgcgtg ccttcgtcgc ccgctcctgg tcgccgatct cgagcacgac
      721 gtccggcgag acctcgaggt tgcgcaccca atccgatcgg tcgccgccgc cggacaggag
      781 gtacaccacg tcctcggcga gcgcgaacca gatctcgatc cgatgcggct cgcccgaatg
      841 ccggccggtg gtcgtcaggt agcagtagtc gaggtcgacg aggggccgac catcggtcac
      901 agcaccgccc agaggacgag cccgacgatc acgagcagtg ccaccccggc gatgatcaga
      961 tccgccttct tgaccttccc cggctgcatc gcgttgaacc ccatcgttag ccctcgacct
     1021 gcccctggat ccggtagtac tcgcggaact tctcgggtga cagccagaag atgtgctggc
     1081 ggaccgtgga cgcgtacgtc ctggcctccg cgccgtcgtg gcacgcccgc tccgacacga
     1141 cgtcgccctc gggactcacg atcgcgacgg tccacgtctc ctcgccgccc cggacctcca
     1201 ccgcgaagcc gttcccgtcc gccgccacgg cgaccaccgt caggcctgcc gaacgaggtt
     1261 ccggtggaac cgtccgagct ggtccgtcaa ctcccccagc acgctgtgct cgtccagggt
     1321 gaacgccggg tggagcgagg ccacccgctc gagcgcgcgt tcgagctcgg tccccagccg
     1381 ttcggcctcg cggagcgatt ccaccaacac agcggcgtaa tccgcgttga agggctgccc
     1441 gtcccaggga cactcgccgc ccgatgccac gacctgttcc gcgaagaact cccggccgtc
     1501 ccgcctgcag gtccccttga tcttcacgcg ctctccttct cgttctctgg cttgttctct
     1561 ggcttgttct ctggctcggt cttcgtcttt gactcggtaa cgagctcgag cagccgatcg
     1621 gccagctcga aggggtccac gggcttcacg aaccacgcgt cggcgcccga ccacttcgcg
     1681 agccaggcgt cgtgcctgcg atccagcagg atcacgatga cccccgcgta gggctccgcg
     1741 tgatcgcgga gggttctcgc gagcccgaac gctccctcgc gtgacgcgat ctcgtccgcg
     1801 acgatcatgt ccggccgctc ccggagggcc gcccaggtgc cgagctcgcc gttgcgagcc
     1861 gcacggaagc ggatctgctc gcccagccgg cgctcgatgc tcctgaccgc gagggccatc
     1921 agctgccggg agcgctcgtc cggggacacc agcaggacat ccacgcgggc gatgctactc
     1981 gcttccacgc agccgccggg cgcggatcgc acggtctttg aacggccccg aacggcgggc
     2041 taggctcggc gcgtcgggac gcggccatgt accttcccac ctgctcgtgc atcgagcagc
     2101 accgggagca accgtcggag cgacgaccga ggaggacgtg atggagagtc tggggaaggc
     2161 ggcggtcgcg gagttcgtcg ccacgttcgc gctgatcttc gtgggcgccg gcgccgtgat
     2221 cgtcgcgagc tccggtcagc tcgacctggt gggcgtggcg ctcgcccacg gcctggtcct
     2281 cgccgtcatg gtctccgtca cgggccacgt ctccggcggc ctcgtcaacc cggcggtgac
     2341 gatcgccctg tgggcggccg gcaagatggg cacgcagcgg ggcgcggtcc tgatcgtggc
     2401 ccagctggtc ggtgcggtga tgggtgcgct cctgctgcgg tacgtcgtgg gtggcgccgc
     2461 gttcgacggc ggaggcgcgg gcgccccggc gctcgcgccg ggcctggccg tgggcaaggg
     2521 catcgtcctc gaggcgatgt tgaccttctt cctggtcttc gtggtgttcg gcacggccgt
     2581 cgacgacaag ggtccttgga acaagacggc gggcttcacg atcgggctca cgatcgcctt
     2641 cgacatcctc gcgttcggac cactgaccgg cgcggcgatg aaccccgcgc gttggttcgg
     2701 gccggcgctg gtgggtggga tctgggatga cgcgctcgtc tggatcgtgg gaccgatcgc
     2761 cggcgccatc atcgccggcg tcctctacac ggccgtgctc ctgcgcggac gggagccggc
     2821 caccccgtag ccccaggccc gcccggtacc ctgggccggc gatggtcgcc gacctctacc
     2881 tcgactcggc ctcggcaact cccctgcttc ccgaggcgcg cgacgccgtc gtctcctccc
     2941 tcgagtccta cggcgaccct ctctcgatcc acgcgctggg ccgcgcggcc cgctccctgc
     3001 tcgacgacgg acgcgagcag gtcgccaccg cgatcggagc ccagcccgac gagatcgtgt
     3061 tcacctccgg cggcaccgaa tcgatcgccc tcgcgatctg gggcggcgtc cgcgccgtcc
     3121 gcgagctcgg cacccggatc gtgatcggcg ccgtggagca tccggccgtg agcggtgtcg
     3181 cgcacgcgct cgagtcggac gggttcgagg tcgtggccgt ccccgtcgac cgagacggtc
     3241 gggtcgatct cgatcgtttc gccgcggagg tgcggactcc cggcacgctc ctcgcatccg
     3301 tccagcacgc gaaccacgag ctcggaacga tccagcccgt cgccgaggcg gcccggctcg
     3361 cgcgcgaagc cggcgtgcgg ttccacacgg acgcctgcca gaccgtcggc cggctcccgg
     3421 tggacgggcc ggccctcggc gtggacctcc tgtcgctttc ggcgcacaag ttcggagggc
     3481 caccgggggc gggcgccctc tacgtccgtc gcggcgtcgc gatcacggcc tatccctgtg
     3541 gggacgaccg ggagcggaag cgtcgctccg ggatggagaa cacccccggg atcgcgggga
     3601 tggcggccgc gctcacggcg tccctcacca cgatggccga ccgtgcggcc gcccactgga
     3661 cgctgaccgc ggagctccgg gaacagctgg ccgaggtgcc cggcacgacg gtccatggtc
     3721 acgcgaccca ccgcacgccc cacctggtgg gattcagcgt cgccggcctc gaccccgcga
     3781 cgctcctgat gacgctcgac gatcgaggtt tccgcctcgg cgccggatca ctctgcagcg
     3841 ggcgtcccga ggacccgtct ccggtgctgg agcagatcgg ctacccgggg acgaccggat
     3901 tccgggtgag tctcggggac ggtgcggacc gatccgacct cgatgccctc gtggggatga
     3961 tctccgggac cgtgcgcgag ctccggcaga tggaggtcgc ttccacggag gcactctcgc
     4021 ggttcagtcc gccgtccgcc ggctgagtcg aggccgtagt ggccctcccc ccgtctggtc
     4081 gctactcgac cgtccaggtg tccccgacgt ggaggagcga gccgaggtcg cccgcgccga
     4141 ggcgctcctt cgcggcctgg agctgatgct ggatggcctg cccgtacacc gggcgctcca
     4201 cctcgtggaa gacgccgatg cacgagggtt cgtccgggcc gcgggagaga tgggccagcg
     4261 cgaacgcgag cgacggccgg ccgtgggcgt cgtggacgac gacgctgtcc ggatcggtct
     4321 ctgccgtggg cgcgatctcg taccggccgt tctcgccgac acgtacacac cggttgccct
     4381 cgtcgaacac gatcggctcc ccgtgctcga gccggatctg gttgtgcttg ccttgggcct
     4441 tgtcgcgcag caggtcgaac gcgccgtcgt tgaagacgtt gcagttctgg tagatctcga
     4501 cgaaggcggt ccccttgtgc tgagcggctc gccggaggac ctcggtcaga tgctgccgat
     4561 cgttgtcgac ggtgcgtgcc acgaacgtcg cctcgacgcc gagggcgagc gcgatcgggt
     4621 tgaacggatg gtcgactgaa ccgaacggcg tcgacttcgt caccttgccc tcttccgatg
     4681 tgggcgagta ctggcccttg gtcagtccgt agatctggtt gttgaagagg aggatcttga
     4741 tgtcgacgtt ccggcggagc gcgtggatca ggtggttccc gccgatcgag aggccgtcgc
     4801 cgtcaccggt gacgacccac actgagagct ccggggccgc gaccgcgatc cccgtggcga
     4861 gtgcgggggc ccgtccgtgg atcccgtgca tgccgtacgt gtccatgtag tacgtgaacc
     4921 ggccgctgca gccgatcccg gacacgaaca cggtcttgtg tggctcgatg ccgagctccg
     4981 gcatgaacgc ctggaccgcg gacaggatcg cgtagtcgcc gcacccggga caccagcggg
     5041 gctcctggtc cgacgtgaag tccttcttgg tcagcgtcga ggcgtcaccc ggtgcggttc
     5101 cgccgttctc gatcacgtca ctcatcgagc accctcgcga tctcgtgctc gagctcctcc
     5161 gcgaagatgg ggaggccctg caccttggaa tacgacgccg cgtccaccag gtacctggca
     5221 cggatcagcg cggcgagctg ccccatgttc atctccggga tcagcacctt ccggtacgcg
     5281 cgaaccacct cgccggtgtt cgtggggaac gggttcagat gccggagatg ggcggtcgcg
     5341 acgctccggc cgtcggcccg cgcccgggcg acggccgccg tgatcgtgcc gagcgaggag
     5401 ccccagccga gcacgagcag ctcggcctcg ccgctcgggt cttcgacctc gagagccggg
     5461 atgtcgtccg cgatgccgtc gaccttcgcc tggcggagcc tggtcatcag ctcgtggttc
     5521 gccgcgtcgt agctgatgtt gcccgtcccg tcctggcgct ccaatccacc gatccggtgc
     5581 accaaccccg gtgtccccgg gatcgcccac ggccgcgcca acctctcatc acgcgagtac
     5641 gggaggaaat cctgcccgtt cggggaggcg aatcccggat cgatcgacgg caggctctcg
     5701 acgtcgggga tcggccacgg ctccgaggag ttcgcgatga aggtgtcgga gagcaagatc
     5761 acgggcgtcc ggtaccgcac ggcgatccgg gcggcctcga gcgcggcggt gaagcattcg
     5821 gccggggtgg acgccgacac gatcggcagc ggcgattccc cgtgacgtcc gtacatcgcg
     5881 agcagcagat ccgcctgctc ggtcttcgtg ggcataccgg tcgaggggcc cgcacgctgc
     5941 acgtcgacga tgatcatcgg cagctccatg atcaccgcga gcccgatcgt ctcggatttc
     6001 aggtcgatgc ccggcccgct cgtcccggtc acggccagtt ggccgctgaa cgccgcaccc
     6061 agggcgatgt tggccgcggc gatctcgtcc tccgcctgga tcgtgaccac gccgaagttc
     6121 ttgtggcggg acagctcgtg gaggagctcg gaggcgggcg tgatcgggta gctcgcgtag
     6181 aacaggggca gaccgctccg ttccgacgca gcgatcagtc cccacgcgag agcggtggcg
     6241 ccgtcgatgt tgcggtaggt ccccggcacc tggggagccg cctccacctt gtaggactcg
     6301 gcgaacagct ccgtcgtctc gccgaagttg tagccggcct tgaaggccgc gacgttggca
     6361 tcgaagatct cctggcggga accgaacttc ttctccagcc agttgagcgt cgtgtccgtc
     6421 gggcgaccga acatccacga cacgagcccg agggcgaaca tgttcttcgc ccgctccgac
     6481 tccttcttgc cgatcccgat gccctccgtc gcccgcaccg tgatcgaggt catcggcacc
     6541 ttgtagacct ggtagccgtc gagggtcccg tcctggaggg ggtcggcggt gtacccggcc
     6601 ttctgaacgt tccgctccgt gaaggagtcc tcgttcagga tgagcacgcc gcccttcccg
     6661 accttgtcga ggtccgcctt gagggccgcc gggttcatcg cgaccagcac gtcggcgtga
     6721 tcgccgggcg tggtgatgtc gttcgaagcg aactggatct ggaaggccga tactccatgg
     6781 acggtgccag cgggggcgcg gatctcagcg gggaagtcgg gcagcgtggc gaggtcgttg
     6841 cccagcaccg ccgtggcgct cgtgaaccgg tcgccggtca actgcatgcc gtcgcccgag
     6901 tcgccggcga accgaacgac gatccgttcc cgctctcgta ccgctttgct catccaagag
     6961 aacaccctac tgcgcgggtt cggctgtgat ggcagtggct gctagtttct cggcgatgcg
     7021 tcgacggcag ctcggcaggc acggcccgga gatctccgtg atcgggtacg gcgcgtggga
     7081 ggccggcgga acggcgtggg gcgccaacga atccgaagag gtcgtgatcg atgcgatccg
     7141 ggccggtctc gacgccggga tcgactggat cgacacagct gaggtctacg gggacggcgt
     7201 gtccgagctc ctcgtcgggc gcgcgatcgc cgggcggcgg gaggagctga ctttggccac
     7261 gaaggtggct cccgcgcccg agggcagcgg cttcacgccg gcgcaggttc ggagcgcttg
     7321 cgagaagagc ctgggccgcc tcgggacgga ccggatcgac ctctaccagc tccactggcc
     7381 ggacgaaacc ggcgtgccgc tcgaggagac ctggggtgcg atgggcgacc tccaggacgc
     7441 cggcctcgtc cggcacatcg gtgtgtcgaa cttcgatcgg ggccagatcg aagcctgcat
     7501 cgcgatgcgg cacgtcgact ccctccagcc cgagttctcg atgctcgcgc gggcgaacgg
     7561 cgacctgatc cgctggtgcg gtgagcaggg cgtcggcgtg gtcagctacg cgccgctcgc
     7621 gtacggcctc ctgacgggcg ccatcaccgc cgagacccgg ttccaggagg gcgactggcg
     7681 cggggcggag caggacgagg gaccgttctc cgacgtcgag gcgtcgctag acatcgtcgg
     7741 caggctccga ccggtggcgg aacggctcgg ctgcggtctc gccgagctcg ccctcgcgtg
     7801 gaacgtgcac cagccgggcg tgacctcggc gatcgctggg agtcgcaacc cggagcacgt
     7861 ccggacgaac gcggcggcgg gcgacatcga gctggacgac gtcacccgga aggagctcga
     7921 caccctgctc gagtgatcag gtgaccggcc cgccgggctg cacctgacgg agtcgcctcg
     7981 cgagcccctc gagcagcccc atcgcgagcg acggctcctc ctcgatcatc cggcgcagcg
     8041 tatgccggaa cagccgtagg agctccatcg gcgtctcgga gacgatcgac gcggtcctcg
     8101 ggccgccgtc caaggccgac agctctccga agaagtcgcc ggggaccagg gtggccacct
     8161 tcttgccccc tctgctgacg cgggctcccc cgctcaggac gacgaacatc gcctcgcccg
     8221 cccctccctc ctccacgatc acccggtcgg cctcgaacgc gacgacttcg gcctccgcga
     8281 cgaggcggag gagatgtcgc ttggagaatc cgtcgaacaa cggcacggcg ttgagggcgg
     8341 ccaccgagcg gcgcacctgg gtcgcggatc gcctgcggga ggaggtcgaa gcctccctcg
     8401 cccgcagcac cgatctcccg gtcggcacga ctcgcatgag cggagggtac cccgacgggc
     8461 cgaggctcgc agccgcatcc gtgctcggcg gtagggttac cgggtggacg tacagagcag
     8521 gttgcgggcg ttgctggccg aacggatcct cgtgctcgag ggtccgaagg gcacggcgat
     8581 ccaggcgctc gagctgtccg aggaggactt ccgcggtgac ctcctgaaag accacccgcg
     8641 cgacatccgc ggggacaacg acgtcctcgg cctcacccgg cccgacatcg tcgagtcgat
     8701 ccaccgcgac tatctggacg cgggcgcctg gatcctcagc acgaacacgt tcaacgggag
     8761 ccccgtctcc caggccgagt acggcctgga gcacctggtc tacgacatga accgtgccgc
     8821 ggccgagaac gcccggcggg cgctcgacgg cgctgaggac cggttcgtgg tcgggtcgct
     8881 cgggccgacg aaccgcacgc tctcgatctc tcccaaggtc gaggaccccg cgttccgcac
     8941 gatcacgttc gacgagctga ccgaggccta tgcggagcag atccgtgggc tcgccgacgg
     9001 cggcgtcgac gtgctccaga tcgagacgat cttcgacacc ctgaacgcca aggcggcgat
     9061 cgccgccgcc caggacgtcg cacccgacct gccgctctgg ctctccttca cggctgtcga
     9121 ccagagcggc cggaacctgt cggggcagac cgccgaggcg ttctggctct cgatcgagca
     9181 tgcggagccg atgatcgtcg gggtcaactg ctcgctcggc gccgcgcaga tgcgcccgta
     9241 cctcgaggcg ctctcgcgca tcgcgcccac ctacacggcc tgctacccca acgccggact
     9301 ccccaacgcg atgggactgc acgacgagga tccggagatc accagcgcct atctcgggga
     9361 gttcgcgcgc gcgggcctcg cgaacgtcct gggaggctgc tgtgggacga ccccggagca
     9421 catccgtcag atcgcggagg cgatccgcga cctgccgcca cgcacggtcc ccgatcccga
     9481 cgagacgttc acatcgttca gtggcctgga gcccttcgtc gtgcgccccg acaccgggtt
     9541 cgtgatggtc ggggaacgcc agaacatcac gggttcgaag aagttccgcc gcctgatcga
     9601 gagcggcgat ttccaggcag cggtcgacat cgcgcgcgag caggtccaga acggcgcgaa
     9661 cctcctcgac gtcaacatgg acaccgacct gctggacggt ccacaggcga tgaccacgta
     9721 cctgaacctg ctcgcgaccg agccggaggt cgcgcgcgtg ccgatcatgg tcgattcgtc
     9781 cagctgggat gtgatcgagg ccggcttgaa gtgcgtccag ggcaaaggcg tcgtgaactc
     9841 gatcagcctc aaggaaggcg aggacgactt cctggacaag gctcggcgcg tgcagcgata
     9901 cggtgcggcc gtcgtcgtga tggccttcga cgagcagggg caggccgaca ccgtcgatcg
     9961 gaaggtcgag atctccgaac gcgcgtaccg cctcctgacg gagcacgccg gctacgaccc
    10021 gtcggacatc atcttcgacc cgaacatcct ggcgatcgcg acgggcatgg aggagcacgc
    10081 cgattacgcg aagtcgttca tcgacgcgac ccgcatcatc aagcggcggt gtcctggggt
    10141 gaagatctcc ggcggcgtct cgaacctctc gttcgcgttc cgtggcaacg acgtcgtccg
    10201 cgaggcgatg cactcggcgt tcctgtatca cgcgcgccag gccgggctcg acatggcgat
    10261 cgtgaacgcg ggacagctcc ccgtctacga cgacatccag aaggacctcc tcgagcacgt
    10321 ggaggacctg ctcttcaacc gacggcccga cgcgaccgaa cggatgctca cgttcgcgca
    10381 aacggtcacg ggcgaggcga tgcggaccga gcaggatctg acgtggcgcg aaggctcggt
    10441 cgaggagcgg ctctcccacg cgctcgtcca cgggatcgac gccttcatcg aggaggacgc
    10501 cgaagaggcg cggcagggct acgagcgacc gctcgaggtg atcgagggcc cgctgatgga
    10561 cggcatgcag atcgtgggcg acctcttcgg cgcgggcaag atgttccttc cgcaggtcgt
    10621 gaagtcggcg cgcgcgatga agaaggcggt cgcctacctc gagcccttca tgcaggagga
    10681 gaaggagcgc ctgggcctcg tcgggtccgc ccagggcacg atcgtgacgg ccacggtcaa
    10741 gggcgacgtg cacgacatcg gcaagaacat cgttggggtc gtcctcggct gcaacaacta
    10801 ccgggtcgtc gacctcggtg tgatggcgcc cgcggagacg atcctggaca cggcggcgcg
    10861 ggagaaggcg gacgtcatcg gtctctccgg gctgatcacc ccctcgctca acgagatggt
    10921 gaacgtggcc acggagatgc agcgccgcga cctcgacctc ccgctcctga tcggcggcgc
    10981 tacgacctcg aagcagcaca ccgccgtcaa gatcgccccg gtctacgccc agcccgtcgt
    11041 ccacgtcgtg gacgcgtccc gggtggtcgg ggtgatgagc gcgctgctcg acgagccacg
    11101 gaaggcgaag ctggacgccg agaaccgcac ggaccaggag cggctccgga ccctacacga
    11161 ggagaagcag aagcagccgc tcctgccgta ccggaaagcc ctcgagaacc gactcaaggt
    11221 ggactggcgc caagaggacg ctcccccgcc gccgttcgtc ggcacccgga ccgtcgagcc
    11281 ggacctcgcc accctctgcg gatacatcga ctggacgttc ttcttcacgg cgtgggagct
    11341 caaggggagc tacccgcaga tcctcgacca tccgcagatg ggtgagaccg cccgagagct
    11401 gttccaggtc gggcaagagg tgctggaccg gatcgtcgac gagcggctgc tcgaggcgcg
    11461 cggggtctac ggttacttcc cggcgcgtgc cgagggcgac gacatcgtga ccgagggcgg
    11521 ctacgtcttc cattgcctcc ggcagcaggg cgaccgcggc gagggagagc ccaacaagtc
    11581 gatcgccgat ctcgtcgcgc ctctggactc cggtgtgcgc gatcacgtgg gcgcgttcgc
    11641 cgtctccgcg ggcttcggcg ccgacgatct cgcgaaggcg tacgaggccg agcacgacga
    11701 ctaccgggcg atcgtgacca aggcgctcgc cgatcgcctc gcggaggcgt tcgcggagta
    11761 cctccaccaa caggtccgcc gcgagtggta cgcgccggac gagcagctct ccagcgaaga
    11821 cctgatcaag gagcgctatc ggggcacccg gccggccttc ggctacccgg cctgcccgga
    11881 ccacagcgag aagcggacct tgttcgagct cttgaacgct gccgaggaga ccgggatcga
    11941 cctgaccacc tcatgcgcga tgatgccccc ggcgagcgtg agcggtctgt acctcgcgca
    12001 tccggccgcc cgctacttcc acgtcgggcg gatcgcgaag gaccaggtcg aggactacgc
    12061 gcgccgcaag ggcgagtccc tcgccgaggc ggagcggtgg ctcgcgccga acctcgcgta
    12121 cgagcctgga tagctcgacc gagggtcctc ggaggcgcgc gtcgctgctg gtagcgtccc
    12181 cggcgtggtg cagtcctact ggcatccgtt cgccgacatg gccgcgatcg ccgcgggggg
    12241 cgagctcgtc ctggaccacg gggacggctg ctacgtctgg gacgtcgacg gcacccgcta
    12301 cctcgatgcg acggcgtccc tctggtactg caacgtcggc tggggccgag ccgagatcgg
    12361 cgaggccgcg gcggcgcaga tggcgaagct gccgacctac tccaacttcg gcgacatcgt
    12421 gaccccggta acgctcgagc tcgccgaccg ggtggcggga ctcgccccgg tccccggctc
    12481 caaggtgttc ctcacgagtg gtggctcgga ctcgatcgac accgcgacga agatggcacg
    12541 gcgctactgg cagctcctcg acgagccgga tcgcacgatc ctcgtccgac gggagaaggc
    12601 gtaccacggg atgcacaccg ccggaacctc gctcgcgggc atcccggcga acgccgccgg
    12661 acacggcgcg ctgatccaag acgtcctgga ggtgccgtgg gacgatcccg actcactacg
    12721 cgcggctatc gacgacgtcg ggcccgagca cgtcgcggcg ttcttctgcg agccggtgat
    12781 cggagcgggc ggcgtgttcc caccgccgga cggctacctc gaggccgtcc gtgcggtgtg
    12841 ccgtgacacc ggtgtgctgt tcgtcgccga cgaggtcatc acgggcttcg gccgatgcgg
    12901 cgattggttc gcctccaccc gcttcgggct ggaaccggac ctcgtcgtct gtgcgaaggg
    12961 catctcgagc gggtacttac cgttgggcgc ggtcctcgcc tcatggggcg tggccgagcc
    13021 gttctgggcc gagggcgcgg ggatgtggcg gcacggctac acctacagcg gccacgcggc
    13081 cgtcgcggcc gcggcgctcg tcaacatcga catcctcgag cacgagggac tgcccgcccg
    13141 cgcgaagacg atggagggcg ccctgctcga cgccctgaag cagctgaccg atcacgacct
    13201 cgtcgaggac gtgcgcgggg gcgtcgggct gctcgccgcc gtccaactcc gctccgacct
    13261 cgtcgaccaa gatccagccc tgggtgcccg cgtcggcgcc gcgacccgcg atgccggcgt
    13321 gctcgtccgg ccgttggtcg ggggcgcgct cgcggtgagc ccgcccttga tcatcgaggg
    13381 ctcgcagctc caggagctcg tggacggcgt ccgcgcgggc ctcgacgcgg tcgcctgatc
    13441 cgcctggacc acgccctccg ctgagcgtac ggtggcgacg tggacacgat cgtccgtccg
    13501 acgacgaccc actccgaacc accaccgata cgtcaccggt gggtacggct cctcatcctc
    13561 gtgtcggcgt tcaccggggc gcttgcgatc acgctcggca ccatgtgggt cacgaactac
    13621 atgccgctcg tacagggaag cgtggcattc ggcaccgccc ggaccgacgt gagggctgtg
    13681 caggtggatg cgttcgatat gcgtggaacg gtgttcacgg ttccgaccgg gcgcaacgcg
    13741 acgttccact accgattctc tctctccaac gagggaccgg ttgccgtccg gatcgagagc
    13801 gtcggcacgc cctcgcggga ggggcctctc acccggcgcg ccgtggaagt gatccccgac
    13861 ccgtggggaa gcgagggacc gatcggctac acgtcggagg agccctggca cccgttcacc
    13921 ctctcccccg gtggagaggc cgtcgtcgtg atggaggcga ggtacgacgg acgctgcctg
    13981 gatcggggcg acggggcggc gtggtactgg gaaccgctgc gttctccgtc ttcgggatac
    14041 cgcggaagga catcgtggag gtcggcgtcg aagtccgatt cagcggcgac gggagttgcg
    14101 agagcggcta gtggaacgag tgcccgcagg cgcacgagct ctgcgccgcc ggattgtcga
    14161 tcgtgaaccc cgaggcctcg agcgagtcca cccagtcgat ggtggcctgg gtcagatacg
    14221 gcacgctcat cttgtccacg acgaccttga cgccgaactg ctcctcgagc aagtccttct
    14281 cggagacctg gtcgtcgagg tacatcgcgt agcggagacc cgagcagccg ccgggttgga
    14341 cggcgatccg tagcgcgatg tccggacggc cctcttcggt gatgagcttc tggacctcgc
    14401 ccgcagcggt ctcggtcagc tggatggacg tacggctggt ctccggtgcg gtctcttcgg
    14461 cggtctgggc catggtgtct ccggttgctg tcatgcccca gattctaccc tcggtcgcag
    14521 tcatccctcg gtgaaatctg cggtctccgc tcccgtcgct cgccggagct gcgaaggcgt
    14581 gagcgcgaac accgagtccg gcgtgcccgc cgccgcccag atctcctgga acgtaagcaa
    14641 tgcggggtcc acgaaggaac gaagaggcgc tgggtggccg aacggcggcg tgccacccac
    14701 ggcgaagccc gtggcggcgc gcgcttcatc cggggtcgcc ctccgcgact cggacgcgcc
    14761 ggcgagcccc gccagcttgg acgggtccac gcgattccca cccgatgtgt atgcgatcac
    14821 cgggttcccg tccgccatga acaccagcga cttcacgatc tgcgcgacgt cacacccgat
    14881 cgcccgcgca gcatcgacgg ccgtcttcgt cccctccggg aaccgccgga tctccggctc
    14941 gagcccgagc tcgcgtgccg cgtccgtgaa gcgttcgatc gctcccatca gaggccgtgc
    15001 ggcagcacga tgtgatcgtc gggcggatcg agcacggccg gtactgtacg attccgcgcc
    15061 gtgagcctgg accgcaccac gcttctcggg atcgcacaag cggagcgcca gcggctcggt
    15121 cggaccgtgc agttcgcgga ccctcagacg tgggagcagc cgagcgccct cccgggctgg
    15181 tggaaccgcg acgtgatggc ccacctcgcc gcgcaggaca ccgctgccgc ccacgtcgtg
    15241 aagggcgacc ctcccgagga actcgaggag tatcggcgtc atctcgacgg ccaaccgttc
    15301 accgtcgacg ggttcaacga gtgggcggtc gagcggcgtt cgggcgcccc gacgcgtgag
    15361 atcctcgtga cgtgggggca agcagcggac gcgctgctcg ggtatgccgg gctcctctcg
    15421 gaggacgagt gggacaaggg acggttcccg tggctcgccg gcgagatcgc ggcccgctac
    15481 ctcatccagt cgcgcatcgt cgagtggtgg gtgcacggtg aggacatgcg cgccacgaac
    15541 ggcctcggcc ccgagtatca gcactggccg atacacctca cgatcgacat ggggatccgc
    15601 atgctcccgt gggccctcgc cgaagcaggt ctggacctca ccggctacag cgtcgaggtg
    15661 gaggtcgacg gagccggagg aggacgatgg cactggggcc tcggtgcagg cgaggtgccg
    15721 cccgccgaca cgaagcccga caccacgatc gtcggccgtg ccccgcacct cgcgctcgtc
    15781 gccgcccgac ggatcccggc cgaggacgtc ctcgcctccg ggaacctcgt gacgggcatc
    15841 gacgcgcagc tcggcgtcac gatcctccgc cacatccgcg cgttcgtctg agggaaccgc
    15901 cgccgcctct cgctcgtacc tcttacgtga acggaacctg tgtaccgttc gtcggagatg
    15961 ccgcccgatg agcgcgacgc cgaccagcgc ctgatgcgct cgttcgctgc gcgcgacgcc
    16021 ggcgcggccg aggtcctgta ccagcggttc gcggggcgga tctacgggct cgggctcgtg
    16081 atgctcggca acgaggcctc tgcgcaggat cttgtccagg acacgttcgt gaagctatgg
    16141 cggaacgccg accggtacga ccgctcgcgg ggcaagctgg agacgtgggt gctgctgatg
    16201 gcccgttcgc tcgcgatcga cgcgatccgg cgccgggtgc tggaggtccg gacgctcgaa
    16261 tccgtcgacc gtccgaccga ggccgaccaa ggtcccggtc ccgacgagcg cgcggtgacg
    16321 gtcgacctga ccgaccgcgc ccggcgcgcc atgtcgtcgc tgccggccga gcaacgggcg
    16381 gcgttggagc tggcgtatct cggtgggaag acgagtgccg agatctccga cctcgagggc
    16441 atccccgtgg ggacggcgaa gacccggatc cgagccgcgc tgctccgtct ccgcgatctc
    16501 ctcgcttcgg acatcgagtc ggccaggggt ggtgatcgtg acgacctgtg aggacgtccg
    16561 tccgcacctc cctgacttgc tcctgggggc cgcgcccgat cccgacgagc tcgacgtccg
    16621 ctggcatctc cgtgggtgtg gtgcctgtcg acgagagctc gacgccctcg aggaaggcct
    16681 ccacgtgttc gggtccacgt tggaacgacc cgccccgtcc gggctgcacg accgcgtgat
    16741 cacggtcttg tccgaggagt gggccggcga gacgaccacg cggggtcggg tgcccgcacc
    16801 gcgtggatgg aggatcgcgg tcgccgcctc gatctcgctc gcggtcctcg cggccacgtt
    16861 cggactgatc cagctcggaa aggcacgtga ggaggcgagg gacgccgcga gcttccgcac
    16921 ggtcctggcg acgctcggcg gcaccggctt caaggtgggc acactccagc cggcgaccca
    16981 gacgccggtc gccggcagcg tcgtggccta cgagtccagc cacgaccagt cgttcgtcgt
    17041 cgtcttcgtg cggacgcccc acctgatggg acgggggtcc ctcctcgtgt ccagttccga
    17101 cggaacgacc tggaacccgg ggccgatcga gttcgaaggt gacggcgacg cggcggcctg
    17161 gtgggtcacc gaccggagca tcgactccat gagcgggctc accatccgct cgcccgacgg
    17221 ctcgacgctt gccaccgcgg gactccgacc ggcctaacgg gagacgacga gccggttcac
    17281 cgagcgtctc ctggcgccga agcggtactt gtaggcgtag tcccccttca ggaagtcgaa
    17341 gaccgcgcac ccatgctcga tcgcctcgcg cacgtcctcc gccacgagga ccatcccggg
    17401 ccccagccgc tgccatcccc gatcgaacgc cgagttgtac agcgagtacg tcccctcgaa
    17461 cacgaagccg atcgtgcccg cggcgagctc gccgttcacg cggatgaacg acagccggaa
    17521 ggtgcctcgc tcgcacagcg cttgccccag ccgccggaag aagatctcca tgccgggaac
    17581 catgaacacg cccttggggc cctcgctcat ccgatgcagc tccacgaacc gatcgaggag
    17641 gggcagcaag gtcccactcg tcgcggtctc gatctggaac ggtcccgcct ccgcctcgag
    17701 cttcttcgcc ttccgtcgga tctcgtggcg gagcttcgag gggatctgct ggaggtacgc
    17761 gtcccacgtg gtagggagct cgaggaacgg cgcgaccccg ttctggtcct cggtctcttc
    17821 gaccgcgagc ccgtgcacca cggctgcgtc ccgcagcgcg gggagccatg cagagccctc
    17881 gggaaggccc cggagatccg cggtgcgcca gtcggcccgg tcgagaagct ccgcccacag
    17941 gcgatcgctg aacgccgtcg ccacctccgg gcgaccgacg ggaccgagat agtccgtcac
    18001 ctccgttcca ccgaggaacc ggagggtgtc gttgatccgc tcgaatgccg cggcagccac
    18061 ctgtgtgccg gcgtcctcgc cgaacgcgag cagcaggtga tcgggctcct cgccgaactc
    18121 ctcccaatag agcttcagga agtccggcgt gtggaagacg gtcccggccg ggtcgactcg
    18181 aacgagctcg ctccagtcgc ggcgcaggaa gtcgcggggg tcttcggaga agaccacgtc
    18241 cagcgtcacc aggagccgcc gcccccgccg ccgcccccgc cgccggatga gccgccgccc
    18301 ccgaacccgc tcgaacccga tgacgccggg gtcgaggcga gcgtcccacc ggtggtcacc
    18361 gcgaagccgt cgatcgagtc cgcgaacgcg gccgcggtta acatccgagg gcccacgtac
    18421 cagcccgacg tgtcgggctg gagccccaga tcctcgaacg ccttcgccca cttcttcgtg
    18481 agcccgaaga cgatcgcgta cggcaggtat cgggtgaaga cgttctcttc ctcggcccac
    18541 cgggccatgt gctgctccgc ggtggcgatc acccgacgga agccacggat gcggcggagc
    18601 atcgctgtcc ccttcgcggt gcgggccggc atctgcctcg ccatcagcgc cagtgcgagg
    18661 ccggcgacga tcacggggac cccgacgatg ccccaatgcg tccagcgggc gagcacgaac
    18721 gtgaccgcgg ccccggcgac gagcaggagc acgcccgctc ccgcccaccg gaggcgcacc
    18781 tggtcggggc gggcgcggaa ccacctctgg cgcatcgcgt ccgaatagag ggcgctctcc
    18841 acaccatgca accggtccga gaaggtcgtc ttcagctccg agaccttcac ctggttcccg
    18901 tcccggaaga ggccgtcgcg gagcttccgt tcgtacgcga ggagctcgct ctcctcgcct
    18961 tccaggcgga tcagggtcca atccgtcttg gagaagaggc cagtgtccgg gatctcctgg
    19021 atcaggagga acccacgtcc ggcgagatcg acgatcgtcg ccgtgacatc gatcacgttg
    19081 gcgcgctcgt cgagcaaggt cccgatctgc cccggacgaa cgttctccgg gggcgcgaac
    19141 tcgacggggg cctccgcgtc gccctctccc ggcgggaccc gctcgctctc gccgctcggg
    19201 ctgcccatca cctggtcgat ctgcgacccg atgaagcggc gatcccgtcc ctctcgccac
    19261 cacagtcggc cgagcaggcc gccgaggagt ccggtcacgg cgatcgcggc ggcgatcttg
    19321 gttccgtcga ggctgaaggc tcggcccgcc gaccagcgct cctcgaggat cgcgctcggc
    19381 tcgggcacgg cacccttcgg gatcgcgacg acgatcgtga ggccctcgta cgccagcaac
    19441 gtccgacccg ccgagaacct cgcggtccgg ccgtcgtgcg tcgccctccc acacacgtcc
    19501 gtgcctccct cccaaccctg gaagcacgcg acccgctgca cctctgcggg agcgcgaacg
    19561 acgacgagaa cgctctcgat cggcgcctgc cactcatcgc cgatcgcgtt ccagtacagc
    19621 tcgtcgtgct catcgaaggc attgagcgca ccttccaccc ggtaggagac ggtgtaggtg
    19681 tgccggccgg agatctcgac gtcgggatcg cccactcgga tcaccgtcga cccgccctcc
    19741 tccgagacat ccacgtcgga cggcgcggtc gggctctcca ccgtcacgtt ccggatcggg
    19801 tacacccggt cgtagacgtc gtcgtggtgg aaccgtgtcg ggatcgtgcg gaagatgccg
    19861 tggcggagcc cggagccgaa gtcgtagtcg atcgtctccg tgaccgccag gtcgccgtcg
    19921 ccgaggatcc ggatctcgac catgtaggaa tggatgccct cgccgacggt ctgagcggcg
    19981 gcggaccctc ccagcgacag gagcagcgcg gcagcagcga acgtcgggag cttcagtgag
    20041 cgcgttcgcc tcggggtcgt cggcacgcgg gcaagggtag acggtcggga cggccgagcc
    20101 gtcagtgggc ggcggcgccg ctcgcggcac catcgtccga gaccgcttcc tgcaggacat
    20161 cccacgccag caccgcgcac ttgatgcgga tcgggtactt cacgacaccc ttcagcgcga
    20221 caaggtcacc gaagacgtcc tcgtccggct cgacctcgcc cgccagcatc ccgcgcacgt
    20281 ccgccgcgag ggacatggcg tcctcgacgg tcttgccgcc caccgactcc gtcatcatcg
    20341 atgcgctcga ctgactgatc gagcacccgg cgccctcgaa ggtgacctcc aggagcttcc
    20401 cgtcctcgag gttggcggag accgtgatct cgtcgccgca cagcgggttg ttcttcgtgc
    20461 acgtcacgac cgcccccggc agctcgcgct tgttccgagg gttcttgtag tgatcgagga
    20521 tcgtttcctt gtagatgtcg tcgagcgcca cggcccctcc gatcgcgact acctgaagaa
    20581 cgcttccgcc ttggccagcg cgtccacgag gacgtccacc tcgtcgcgcg tgttgtacac
    20641 gtagaacgac gcccgagcgg tcgccggcgt ctcgaaccgg cgcatcacga gttgcgcgca
    20701 gtggtgcccc gcccggatcg ccacgccctg ctcatccaac accgtggcca ggtcgtgagg
    20761 gtggacgtcg cggaaccaga agctcaccgc gcccccacgg gcggccgcgt ccttcggccc
    20821 gtagaccgtg gccccggccg acagcagcgt gtcgagcgcg tagcgcgtga tctcctcctc
    20881 gtgcgccctg acggcgtcca tgccgagggc ctggaggtag tccacggcga cgccgaggcc
    20941 gacctgctgc gcgatctgca tcgtcccggc ctcgaacttg tacgggatgt ccttccaggt
    21001 ggagtggtcg tggaagacct cgtggatcat ctctccgccg ccgaacatcg ggtccatggc
    21061 gtcgaggagc tcggccttcg cgtacagtcc accgctcgcc gtgggaccga gcatcttgtg
    21121 gccgctgaac gcgaggaaat cgcagtccag ctcgccgacg gacaccccgt tgtgcggcac
    21181 gagctgcgcg gcgtccaccg cgacgagcgc acccaccgcg tgggcggcct cgacgagctg
    21241 gccgagcggg gtgatcgtgc ccaaggcgtt ggacatcccg gtcaccgaca gcatcttcgt
    21301 tcgctcgttc aggagcgaac ccagctccga gaggtcgagc tgcccgtcgt ccgtgagcgg
    21361 gatgtagcgg aggaccgcac cggtcacctg ggcagtgagc tgccagggca cgatgttcga
    21421 gtggtgctcc atgtcggtga ggaggacctc gtcgccctcc ttcaggaact tccgccccca
    21481 tccctgggcc acgaggttca gtgattcggt ggtcccccgg gtgaacacga tcgtgcgagg
    21541 gtccggggag ccgaagaacg ccgcgagctt cgcgcgcgcg ccctcgaagg cctccgtcga
    21601 ctcctcaccg agcctgtaga tcccgcggtg ggcgttcgcg ttgtggttcg cgtagaagtc
    21661 ggagacggcg tcgatcacct gtcggggctt ctgggacgtg gcggccgaat ccagatagac
    21721 gagcgggtgg ccgttgatcg tgcgctccag gatggggaag tcgcgccgga tccgctcgac
    21781 gtccagcacg ctcgtggcgg tgccgagctg ctctttggcg ctcatccctt ctcctctccc
    21841 ccgtcctcga tcccgagctc ctcgcggatg ccctcgtagc ccttctcctc gagctcgctc
    21901 gcgagctccg ggccgccgga cctcacgata cgaccctgca tcatcacgtg gacgcgatcc
    21961 gcggcgatgt agttgaggat ccgctggtaa tgggtgatca gcaggacccc gagatccggg
    22021 cccacgagct cggccacgcc gccggccacc agcttcagcg agtcgatgtc gaggccggag
    22081 tcggtctcgt ccaagatcgc gatctccggt tggagaaccg cgagctgcag gacctcgttc
    22141 cgcttcttct cgcctccgga gaacccctgg ttcacgtagc gctgcaccat cgcgtcctcg
    22201 atgccgagac ggtccatctg ctccttcatg tgcttgcgga acgcgagcgc gctcatctgc
    22261 tcgcccttca cggcctggta ggcggtccgg aggaagttga cgacgctgac gccggggacc
    22321 tccgtcgggt actgcagcgc gaggaacagc ccccgcttgg cccgctgatc ggtcgtgagg
    22381 ccggtgatct tctcgccctt gaagatcacg tcgccaccgg tcagcacgta cccgggccgc
    22441 cccatgatga tgttggagag cgtcgatttc cccgagccgt tgggtcccat cagcgcgtgg
    22501 gtctctccct gtgtgacggt caggtcgatg cccttcagga tctccttgcc ctcgacttcc
    22561 gctcggagat ctcggatctc gagcatcgcg cggccgttgt tctcgctcat gtcttcacac
    22621 ctcgatgtag atgtcgtcgt cctcggtggt gatcgggaag acgtccaccg gcgtcgtcgc
    22681 cggcaggctc ctcggcttcc cgctgttcag atcgaacgag gacccgtgct tcgggcactc
    22741 gatcgtctcg aagtccgcgt cgacgtcccc ttcgtcgagg aagtagtgcg cgtggctgca
    22801 gatcgcatcg atcgcccgga gaccttcgtc cccgaggttc acgagcgcta cgaggcgtcc
    22861 gtcgaccggg aagcgcttcg cttcaccggg cgctggcgcc tcggcgacgt cgcagacctt
    22921 gaccctcccc atcagttcgg gtctccctgg ccgagtgccg cccgatcgag ctcgtcctgg
    22981 atcgcctgct cggcgccctc gcgcacctcg tcgatcttca cgcggtccag gacctcctgg
    23041 aagaagcccg agacgatgag ccgttcggcc tccgcgcgag ggatcccgcg gctcgtcagg
    23101 tagaagatcg cgtcctcgtc gaccgggccg acgctcgccg cgtggccgca gcggacgtcg
    23161 ttcgcctcga tctcgaggtt ggggatcgcg tcggccttcg catgctccga gagaacgatg
    23221 ttgcgcgacg tctgcatcgc gttcgtcttc tgcgcgtcgg gccggacgtg gacccacccg
    23281 ctgtagaccg cacgggagtg atctcggagc gcgcccttgt agagcaggtc gctggtgcag
    23341 ttgggcgcca cgtgatcctg gatcgagcgg tggtcgaagt gttggtcctc gtcggcgaag
    23401 aagataccga gcatctcgct gaacccgccg cgctcggcaa ggatcgtctc ggcctcggcg
    23461 cgcgcgagcg aggccccgaa cccgacgttc aaggtgcgga gcgttgcatc tcgcccgacg
    23521 gttgcccgct ggatcccgag gtgggtgacg ccgctccccc actcttggat cgagacgtag
    23581 cggacgtggg cgccgtcgcc cacgtggatc tcggtgatcg cgtcgctgaa cgcccgacgt
    23641 aggtcggggc tcgcgtaacg gtcgatgaac gtgacctcgg agcccgcgtc gacgacgacc
    23701 agcgtgtgcg ggaagacggc ggccccgtcg gcatcgaggt aggtcagcgt ctggatcggg
    23761 agctcgagcg ccgtgtcgcg ggggacgtgg aggaacgtcc cccccgtccg gaaggccgcg
    23821 tggagcgccg tgaacttcgt ccggccggcc gggacgagct gatggaggaa cggctccacg
    23881 aggtccggat gggtctccga agcctcgtcc aagctgccga accagacgcc gcggtcggtg
    23941 agcgcggtgc tccgctgggt ggagagcacg gtggagttcc gctggatctg aaggccggct
    24001 cgttcgccgg tgacccccgc ggagccgagg agcccatgcc ggttgaggtc ctcggcaccc
    24061 ccgccgtccg cgagcggaag gaagtcgagc tcgaaggccg acaggtccgt atagcgccac
    24121 tcctccgtct cctgcgaggg gacgggcatg gcctcgaagc gctgcagggc atctgctcgc
    24181 agctgttcca cgaagccggg cgcccggggc agcgactgca gggcgcgctc gtcgaacgtc
    24241 gtggtgctca ccgcagccat ggaggaatcc tatcggagcg gtaggattcg ccccaaccgg
    24301 ggcacccggt tccgccggaa gcatgcgcgg gaccggcacc tgcgctcggc gcgctcctgg
    24361 ggaaagatgt ctccaccgtg cgatgacccg gcccggggag gagggggtga cgaccacgac
    24421 gcaggactcg gccggagcgg cgctgcgagc gttcatgatc gccgacatcc ggggctacac
    24481 gcggttcacg cgggagcatg gtgacgccgc cgcggcgcgt ctggcgggcc gattcgccga
    24541 gctcgcgcgc gacgccgtcg aggcgcgggg cggcgaggtg ctcgagctcc gtggcgacga
    24601 ggcactcgcg gtcttcggct cgacggtgcc ggccgtccgg gcggcgatcg agttccaagc
    24661 gctctgcgcg gaggagttcg gggaggaccc gacactgccg ctcgccgtgg gcatcggcat
    24721 cgacatcggc caagccgtcc cggtcgaggg tggcttccgg ggggtggcgc tcaacatggc
    24781 ggcccgactt tgctcgaacg ccgcggccgg acaggtgctg gtgagccggg cggtggcgga
    24841 ctccgcatcg gacgccgagg gcatccggtt cgagagccgt ggggacacgg cgctcaaggg
    24901 gttcgaaggc gccgtcccgc tgatcgaggc cctcgacgag ctgccggcag cccctccggt
    24961 cccggcgacg gcgacggccc ccggcgatgc gctcgccccc gagctcgacc cgatgacccc
    25021 cctcgccgac cgcgtccggg agatgcgctg gctccgcgga acgtggcgtc aagcgcgccg
    25081 cggtcgtggt cgcgtgctct ggatctccgg cccatcgcag atcggcaaga ccaggctgac
    25141 cgcggagctc gcctcgatgg tccacaccgc cggaggccgc gtctcctacg cggggctcgg
    25201 cggaaccgcg gacgcggagg cgctgcgggc catccgaggc atcacggatc atgcccaacc
    25261 ggccctactc gtcttcgacg acgtcgatgc gatgggggac gaggtcgctc gtgagctcga
    25321 ggagcggctc gacacgatcg ccgttcggcc cgcactggtc gtggcgacga tccgagcgcc
    25381 ggaggtctct ccctcgatcg agcgcgtcct gcggcgctcc gatccatccg gcgacgggct
    25441 tcgccgactc gcccccctgg acgcggatgg cgtgcggcag atcgccgccg actacgtgga
    25501 cgtcgcggtc gacgaggtgc cgctggaagc gttcgtccgc gcatccggcg ggatcccggg
    25561 acgggtgcac gaggtcgtcg gcgagtgggc gcgcgaggag gcagggcgcc ggctcgcggc
    25621 ggcggccgag tggctctccg aaggacgggc ccgccgcaac gccgacatcg cgttcgccaa
    25681 caacgtgatc ggactgaagc tcggtcgcct gtacgcgccc gcggagggtg acgacaggtc
    25741 cgtctgtccg tacaaggggt tggccacgtt cgagcagggg gacgctgcgt tcttcttcgg
    25801 gcgggagcgt ctggtcggag acctcgcggc gcgaaccgtg ggggcggggc tgctcggcgt
    25861 ggtcggcgcc tcgggcagcg ggaagtcctc gctcgtgttc gccggcctgc tcccctcgct
    25921 ccgcgccggc ctccttcccg ggagcgagcg ctggcgcgcc gtgaccatgc gtcccgggga
    25981 gcatccgctc gcggagctcg aggtagccct cgccggcacg tccacccccg gcgacccggc
    26041 ggaggcggtg cgttcgatcc cacccgagga gcgactcgtc ctggtcgtcg accagttcga
    26101 ggagatcttc acgacctgcg aggacgagga cgagcgcggt cgatcggtcg cggcgatctg
    26161 cgggatcgcg aggagcgctc aggatcgtgt cgtcgtcgtg ctcacgatcc gcggggacta
    26221 ctacggtcac tgcgcgcggt acccggagct ggccgagctc ctcgctgcga accacgtcct
    26281 ggtggggtcg atgacgcgcg aagagcttcg ccgcgcgatc gagctcccgg cccggcgcgt
    26341 cggcctccgg gtcgagtccg tgctgacgga cacgctcgtc gaggagatcg ccgaccagcc
    26401 gggagccctc ccactcctgt ccaccgcgct ggtcgagcta tggcaggaga gcggggcact
    26461 ggctccgcct cgagacctac gagcgaacgg gaggcgtccg cggcgccgtt gcccgcatcg
    26521 cggacagctc gttccatcag ttgtccgacg cccagcagga ggccgcgcgc gcgatcttcc
    26581 tccgcctcgt cgcggccggc gaggccgagg ggatcaccag gcggcgggtc cgcctcgacg
    26641 agttcgacct cgagcgggat ccggcggcgg tcgcggcgct cacacagctc acccgcgatc
    26701 ggttgctcac gatggacgcc tcgacggtcg aggtgtcgca cgaggcgctc ctccgggagt
    26761 ggccccgcct ccagggctgg ctcgcggaag acgcgaccgg acgccagctg cgtcggcacc
    26821 tgacatctgc cgcgaggcaa tgggagggct cggaggaaga cgactcggag ctctaccggg
    26881 gagcgaggct ctccgccgcg ctcgactggt cctccgggca cgagcgggag ctgaacgaac
    26941 gcgaacgcgc cttcctgtcg gccagccggc gagccagtga gcgggaggcg gaacgacaga
    27001 gacggaccaa ccggcgcttg cgaggctcgc tcgcgggtgt ggccgtgctc ctcgcgatcg
    27061 cgctgctggc aggttccctc gccctcgtgc agcgaggaga ggcacgcgac gccgccgccc
    27121 gggccgggcg atccgccgtc acggccgatg ctcagcgtat cggcgcgctc gcgctcctcg
    27181 aagaccagcc cgacctcgcg ttgctgctcg ccgtccaggg gacgacgctc gatcccgagc
    27241 cgccggagac gagagggtcg ttgttcgaag cgctgatgag gttcccgcgg gtcctccgcg
    27301 tccttcccat gcgggagcgc atccttcgga tggcggcgcc gacgcccgcg ggcgacgttc
    27361 tcgccgtcgt ggacaacacc ggtcgggcgt tcgtcttcga ggcctcgtcc ttcgacccga
    27421 tcttcgagga ggcgatcggc ggctcgatca accagctggc gctttcaccg gacggtcgac
    27481 gcctcgtcgc gtcggtgtcg gtcgacgacg tccaggagac gctgatcgtg gcgttcgacg
    27541 tcgcgtccgg gcggcgagca tggtcgttcc cggtgccgca cgccgacgcc gggacgggga
    27601 ggctcggtgt gtcacccgac ggcgaggagg tgatctgggt ctcgcggggc gcggtccgtt
    27661 tcctctccct cgggagcggc gagctctcgc gatcgcccgt ccgggtcgag ggcgagggcg
    27721 cgtggatgct ggatcggcac cggctgctca ccttcgatga cgggaagggg gtgcggatcc
    27781 gagacgtgag gtccggagcg ctcctgcgcc acatcgtggt cgagggtcct cggggggcag
    27841 gccaggccct cgctcccgac ggctcgctgc tcgcgctcgg cggccgggac ggctcggtcg
    27901 cggtcctgca cctcgacacc ggcgagctcg tgcagatgga aggcacccat gacggctcca
    27961 tcgagacgat gctgttcgcg ccggacggct cggtcctggc gaccggtggc gaggacggac
    28021 acgtctccct ctgggatccc acgacgggag cccgcgagga ggatctggtc ggtcacagca
    28081 gtgggctccg cgccgcggcg ttcacgcagg gcggccggac gttggtgagc ggtgggctcg
    28141 acagcacact gatcgcgtgg gacgtgaccg gggagcgagc gatggtgcgc accttcgcgc
    28201 ggccgggacc tccctgcccc gaggggtgct cctccccgca gttctcggtg acggcggacg
    28261 gtcggtcgct cgcgatgatc gggaccgact tccgggtccg attcctggac acgacgacgc
    28321 ttcggccggc ggggacgctg tcggatccgg gtctggcctg ctgccagata cccggatcga
    28381 gcccggacgg tcgaagatcg tcacggtgtt cggcctggct caggccgggc tcccgggacc
    28441 cgatgcggtc tccctgtggg acgtccgtac ggggaagctg ctgcggcatc tgttcacgag
    28501 cgaggagcca tggaccgacc tccagcacgc acctcccctc gatacggtcg cctacgcccc
    28561 cgacggcgcc tcggtggcga cgaacgagag cgaacggatc cacgtcctcg atccccagac
    28621 gggcgagacg ctcgcgacgt tcgaggcgcc tgccttcgtg cagtggctct cgtacagccc
    28681 ggacggccgc ctcctccagg ccgagatgga cggaggtctc gtcgccgtct gggacctgcg
    28741 gacggagcgg atggtgtggt cccgcaaggt ggacgaccag acacccctcg ggggcgagtt
    28801 ctcggccgac ggcaggtatc tgatcctcgg gagcgaatcg ggcaagatcc atctcctcga
    28861 tccgcggacc ggccggccgc ttcgggatcc gctgctcgcc cacgacgcgt acctggcttc
    28921 gatcaccgtc catcctgact cgtcgatgtt cgcgagctcc gggaccgacg ggctcacgtt
    28981 cctgtgggac acgtcgacgc agcgagcgct cgggtcgccg ttcggtgtcg ccggaggcgg
    29041 cggggtgtgg agccgtttct cgcccgacgg gcgagtgctc tacgtgtcga ccgccgagcg
    29101 cggtttcgtc ttcgacacga cgctggacac ctggatcgag cgcgcctgct cgatcgccgg
    29161 tcgcaccctc acgcgcaccg agtgggagcg cttcctcccg cagcgaccgt accgtcccgc
    29221 ctgcaggacc acctgagcag ggttcgacga gatggtccat ctccctggga tcctccgccg
    29281 atcgtcacgg ccgtcactgc gggtcctcgt tcaggagacc gggtttccga gagagatgag
    29341 gatcgagcga cgccgaccct cagccgaccg agccctccat ctggagctcg atcaggcggt
    29401 tcagctcgac cgcgtattcc atcggcagct cgcgcgtgat cggctcgatg aatccgttca
    29461 cgatcatcgc cgtggcctcg gcctcggaga gccctcggct catcaggtag aacagctggt
    29521 cgtcgccgac cttcgagacg gtagcctcgt gtccgagggc ggccgtctcg tcctcgacct
    29581 cgatgtaggg ataggtgtcc gacctcgatt cgtcgtccag gatcaacgcg tcgcagcgca
    29641 ccgtggactt cgcgtggtgg gcatcgggct cgacccgcac gagaccgcgg taccccgtgc
    29701 gaccgccgtc cttcgagacc gacttcgagt tgatcacgct cgtcgtgtac ggcgccgcgt
    29761 ggatcgcctt gccgccggcg tcggtgtgca tgcccttgcc ggcgaacgcc acggacagca
    29821 cctcgccgcg ggcgcccttg ccgagcaagt agatcgacgg gtacttcatc gtcaccttgg
    29881 agccgatgtt gccgtcgatc cactccatga cggagtcctc gtaggctgcc gcgcgcttgg
    29941 tgaccaggtt gtacacgttg gtcgaccagt tctggatcgt cgtgtatcgg acgcgggcgc
    30001 ccttcttcgc gacgatctcc accaccgcgg aatgcaacga gtcgctcgtg tagatcggcg
    30061 ccgagcatcc ttccacgtag tgcacgtacg agccctcgtc ggcgatgatc agcgtccgct
    30121 cgaactggcc catgttctcg gcgttgatcc ggaagtaggc ctggagcggg atgtcgacgg
    30181 ccacgccggg cggcacgtag atgaacgagc cgcccgacca caccgcggtg ttcagcgccg
    30241 cgaatttgtt gtcgttcggc gggatgatcg tcccgaagta ctccttgacg aggtccgggt
    30301 agtcgcgaag cgccgagtcc atgtcggtga agagcacgcc gatgtcgtcg agctccttct
    30361 tgatcttgtg atacacgacc tcggactcgt actgcgcgct gaccccaccc aggtacttct
    30421 tctccgcctc cgggatcccg agcttgtccc acgtgccgcg gatgtcctcg gggaggtcct
    30481 cccagttctg agcctgcttc tcggttgagc ggatgaagta gtagatgttc tcgaagtcga
    30541 tatccgagag gtcggcgccc caccagggca tcggacggct ctcgaagtgc ttcagggcct
    30601 tcagccgata cttccgcatc cagtccggct cgctcttgag ctcgctgatc tcctcgacga
    30661 cctgcgtcga gaggccccgc ttgggctcga agaccgagtg ggacgggtcc ttccagtcga
    30721 aacggtatcc cttggaaagc tccttgagat gctcttcctg gctgacggcc atctcgctcc
    30781 ctcggatcgg ctgctgcggt cccctaccgg gtacccgtta agtctatccg gtaggtcgga
    30841 ttcgtctcag ccgtgcctcg cggccacgtc ggctggatcc cagcggtcga cccgacgagc
    30901 gagcaccttg gtgaccaccg accccacgac caccgtcagg atcacgtagg cgaccgtcaa
    30961 cgcccccagg tcctcgtcga ccgcggcggc cgtcgccagt ccggcgatcg cgatcgagaa
    31021 ctcgccgcga gggatcagtg cggccccgac caccccacgt gcccgctcct ccgagccgag
    31081 atgccgtgcc gcccaccacc cggtcgcata tttcgtcccc gacgtcacga tcccgaggag
    31141 gaccgccatc gcgaggaccg gagggatggt cgatgggtcg accgagagtc cgatgaacgc
    31201 gaagaagatc gccgcgaaga ggtctcgaag cggtcggacg agggcctgcg cgccttctgc
    31261 cgcaggaccg gacagcatga tccccgccaa gagcgccccc accgccgcgg agaaccgagc
    31321 cacgtcggcg acgccggcga tcaggatcgc gaagccgagg atcgtcagga gcagcgcctc
    31381 gtcggatcgg ctgaacagca tctggctgag acccacctcg acgcggagcg ccacgacaag
    31441 cagcagcccg acgacgagga tcgccaccgc cgctgccgcc agcgaggtca gcgcttcgtc
    31501 cccgatcagc agggccgcga gcaccgggag gtagatcgcc atcaccaggt cctcgatcac
    31561 ggcgatcgtg accacgaagc ggctcgcgcc gcgcgcgtgc ggccccgcgt gctcgaggag
    31621 cttcgcggcc acgcccgagg acgagacgta cgtgatgccg cccaggaacg ccgcagcgag
    31681 cgggccccag cccaggatga gtccgatgag caggcccggc gtgaagttca gggccaggtc
    31741 gaccaccccc ggcgctgcgg acgccttcgt tgtggcgatc aggtcgcgtg acgaatgctc
    31801 gaggccgagc acgaacagga ccagtatcag gccgatctcg gcgccggtct cgacgaaccc
    31861 gcgggtcgtg acgagtggca ggatgccgcc ctcgccgaac gcgagtccgg ccagcaggta
    31921 caggggaacg gtgggcaggt cgaaccgggc ggcgatccgc gccaatcccg cgagaccgac
    31981 gatcacggcg ccgagctcca gcaacctcac ggagcgcgac ctcctgacgc gagagccatc
    32041 gaccgagtct aggagtcgtt cccgtggcct cgcgtccgga ccgacgcgct gctacggtcg
    32101 ccgcgacgag tgggggtgga acggatggat gacgagcggc gcgtgcaggg gcaggtcgag
    32161 cgagcggcgg ccacgggagc gttcgtggga ggcggcacgc tcttcacgga acccgtcctg
    32221 gtggtcaacc agaagacgaa gatcatcgag ctcaccaacg agtactcggt gttcgacgcc
    32281 gccggcatgc aactcggggc ggtccgtcag accggccaga ccacgctcaa gaaggcgctc
    32341 cggctggtgt cctcgatcga ccaattcctg acccacacgc tcgagatcac cgacgccgcc
    32401 ggcgtgacac agctcacgct cacgcggcct cggaaggtct tcaagtcgac gatcgtcgtg
    32461 gcacgtcccg acggcgccga ggtcggccgg atcgtgcagg agaacatggt cggcaagatc
    32521 cggttcggga tcgagggcgg tggctcgcgg ctcgggggga tccagggcga gaactggcgg
    32581 gcatggaact tcgggatcac cgacgcggcc ggcaccgaga tcgggcgcat cacgaagacc
    32641 tgggagggct tggcccggac ggccttcacg accgcggacc actacgtcgt gcagatccac
    32701 aaggagctgc ccgagccgtt gcggtcgctc gtcgtcgcgt cttcgctctg catcgacacc
    32761 gcgctgaagc aggacgagcg cggcttcaac tagggcggcg acgaagaggc cgacgccttc
    32821 cggatccgtt acccgagctc caccggggcg ccgacgagcg agccgtactc ggtccacgag
    32881 ccgtcgtagt tcttcacgtt ctcgaagccg aggagctcct gaagcgcgaa ccacgtgtgg
    32941 gaggagcgct cgccgatccg gcagtacgcg atcacctcgc cctcgcccgt gatcccctcg
    33001 gccgcgtaga gctcgcgcag ctcgtccgcg gacttgaacg tcccgtcctc gttcgcggcc
    33061 ttcgcccacg ggatgttggc cgcgccggtg atgtggccgg ggacctgcgc ctgctcctgg
    33121 ggcagatggt ccggcgcgat cttctcgccg cggaactctt cgggcgagcg tacgtccacg
    33181 aacttcgttg agccggcctt ctgcaacacc tcgtcgcgga gcgctctgat ctccggtcgt
    33241 tccgcgcccg agatcgagaa tcccgtcgcc tggtaagacg gcgcatcctg ggtgagctca
    33301 cggctctcca gctcccactt cttccgaccg ccgtcgagca gcttgacgtt gtcgaacccg
    33361 cgaagcttca ggagccagta cgcgtaggcc gcgaaccagt tgttgttccc gccgtacaga
    33421 acgaccatcg tgttgggccc gacgccggcc gccgacagga gctcggagaa cccggccctg
    33481 tcccgataat cacggccgac gtccgtgtgc atgtccgtgg tccagttcca cgcgaccgcg
    33541 ccccggacgt ggcccttctc gtacgcagcg gtgtcctcgt ccacctcgat cgcccgcacc
    33601 gcggggtcgt cgagatgctc ctccagccag tcggtgctca cgagcacgtc gggcttcgcg
    33661 tagtccgtca tcgcctgttc ccctctctcc acggccccgc gaggggaccg gaaacgcgtg
    33721 agcggccgca ctcgcggccg ctcgtgtcac caggaatgtc cttcgtcgct acgtcgaccg
    33781 cgcgcgcagc gaggctccgg ccggcgccga acagcagagc ttggacggga tcatcgctcc
    33841 cacgatgccc agcgctcggg tccggaccac cctcgaatcc cacccctcag gtcggaactg
    33901 caggtgcaac gactatcgca ccgcggagac ggcgtcaagg tcggtccgac cctagaacat
    33961 cccgagggcg gccttggcct cctccgacat catctcggga ttccaggccg gcctgagcac
    34021 catctcggga cgcacctcgc cgatcccgtc gagcggggcg aggaacgcgc gcatctggtc
    34081 ctcgatcagc ggcccgatcg gacaccccat cgtcgtgagc gtgtactgga tgtcgacgtc
    34141 gccgtcctcg gccacgtcga cctcgtacac gaggcccaga tcgacgatgt tgatccccag
    34201 ctcggggtcg atcacgctct tgagggcctc gaggacctgc tcttccgacg gcgctcctcc
    34261 gttcgtgcgc accgtgccgg tggatgcggg ctcgggcgac gcctcaggag cctcctgcgt
    34321 tcgcggtatg gaggtttcgg gttcagacat gcatacctcg ctccagatac gaactatcct
    34381 tagcacccag actaccgtcc agatcgtgaa ggcacgcgac aggacgcgcg acggaccgaa
    34441 ggggggccgc atccgaccga tgactgggat ccgggcgttg atcgtggacg accatcccgt
    34501 gacccgggaa gggcttcgga ccgccctgtc catgtccgat gacgcggtgg tggtcgtcgg
    34561 agaggcggcg agcggccagg aggcgatcga ccaggcgcgt gatctgtctc ccgatgtggt
    34621 cttcatggac gttcggatgc cgggcatgga cggcatcgag gcgacccgcc ggatccgcga
    34681 ggcctctccg gagacgaggg tcatcctcat cacgatcgac gagtcgcgcg gagcgatctc
    34741 cgaagcgatc caggccgggg tgtcggggta tctgctcaag gacgcgtcac ccgatgcgct
    34801 cgtcgatgcc gctcgcaacg cggtcgaagg caacgccgtg atccatccgc agctcacgaa
    34861 gaccttcatc gaggaggtcc acctcgtcga ggccgagggg aggaccaccc cgctgtccaa
    34921 acgcgagcgg gagatcctcc agaaggtggc cgacggggcc acgacgaggc aggtcgcgag
    34981 cgatctcggg atctcccccc acacggtgaa gacccacctc gaacggatct tcgagaagct
    35041 cggggcgaac gaccgggcac aggcggtggc gatcgccatc cgcaccggca tcgtccactg
    35101 aacggccgac ctcaaccggc cacctcaggc gccgatacaa cagcagatgc ccaccaccgt
    35161 gctgctctgc gacacgtccg acgaggtcgc tcgcttccag taccacctgc tgcgggagca
    35221 cgacctggag gtcgaggtca cgaccgatgc cttccggggc gtggaggcgg cggtccggtc
    35281 acggccggac gtgatcgtgt ccgagctcac gctcgagggg ctgggcggcg ccgagctggt
    35341 gaagcgattc ctcgcctcgt ccccctcatc ccggttcgtg gcccacaccg tggttcgcga
    35401 cccggtccgc gtgaccgagg tcctcgccgc cggcgcttcg ggttacgtgc tgaaggaaga
    35461 tcccgtggag gactccctcc gcgccatccg cgcggcgtcc acgggcgcgg tcaccctctc
    35521 cgggcaggtg gccgggctgc tcgcgaccga gctcgcgcgc gcggtcagcc gttcccgcga
    35581 gctggagacg gagctcgtcg cgctccgcca cgacgtcgcc caggggtcca cggcgaaggc
    35641 cgacttcctc gcgaacatct cccacgagct ccggacgccg gtcaccgtgg cgaagggcat
    35701 cggctacgtc ttgaagaacc cggccgtgcc cgacgaggag cgcaacgagt tcctcggtca
    35761 gctcacgggc tcgctcgaca agctgatggg gatcgtggac gagctgatca ccctttccga
    35821 gctcgagcgc ggaacgttcg agctgaacct cgtcgagacc gatctcgcgc cgctcgtccg
    35881 ccacgcgatc gacgagctcc ggatgcgcta ccccgggatc ccgatcgcgg cgaaggtgtc
    35941 cgacggtctg ctctgcatcg ccgacgggcc gcgcctcggg tcggtggtcc gggagctcct
    36001 cgacaacgcg tgccgctact ccccgttcgg tcgcgccgtc gagctcgtgg cgcgggacct
    36061 cgccgagggc gtcaccgtga tggtgaccga ccggggtgag gggctcgacc gaaccgtcgc
    36121 ggcccgggcc ttcgaccagc cgttctccac cggcgaaggc gtgctccaca aggagaagtc
    36181 gggtgtgggg accgggctgc acctcgcccg gcagctggtg gtcgagcacg gcggcgtgat
    36241 gtgggtcgat cccctgccgg gcggcggtac cagagcctcc ttctgcgtcc cccaccgccg
    36301 gggcgaaagg gtcgaggccc ctccggcgca cgtcgcctga atcaccgccc cccgttggac
    36361 ctacggaggt gccgggaagt atcctggccc cgaggatcag gtgcgggtgc gcagaagcct
    36421 cctcgggatc ggggtcgtcg tcgtggtgtc cgcatgcacc tccgcgtccc ccacgacctc
    36481 acctctccaa gagctcctcg tcacgccgtc gccgacggcg ttcgacctgg tgacagccgg
    36541 ccaggtccgg ggagtcgttc ccgaccgctg ggaggccacg ctcgtggacc cctcgtcggg
    36601 gatccgggga gggttcgtgg cgtcgccccg gcagggacga tggtcggatg tcgacgggag
    36661 gacggtaggg atgtcggcca cgtgggtcga tgccacgcgt gtgggcgttc cgtccgactt
    36721 ctactacctc gcggcgaacg gccccctgct ctcccggctg accacctccg ccaactgccg
    36781 gggttcgcgt cacgtgtatc tcgaccgtcg tccgaccttc gatctggaaa gccattcccc
    36841 ggggcacttc atggcggagg gagcgggcgt gtgccacggc ggcgcgaggg cgacgcggtt
    36901 cgcctacttc gtcgccgcgc cgggattcgg ccccgtccac cgggtgggga tcccgagcag
    36961 cggcctctac gtggtggtgg ccgtcgtcaa ccagcggcgc ggcgcgaaga acctcctcga
    37021 ggagctgatg cggcgaaccc gcttcggtga cgacgccatc caggacttcg tggccatggc
    37081 tccccccgcg cagtcggcct gacgggcccg agagccgcta gcgcgggctt cggtccacga
    37141 acggcggttg caccacggtg gccgcaccgc gccttccccg gatatccacc tcgacctctt
    37201 gaccgagctc cacgacgtca cccggccaga ggtacgccag cgcgatcccc gtaccgagcg
    37261 tcggtgagaa cgtcccgctc gtgacctccc ccaccagttg gtcccccacg aacaccgggt
    37321 agtgcgccct ggggatgtat cggcgctcgt gcatccggag accgcgcaac cggctgggga
    37381 gcccctcctc ttgctgccgc aggagcgcgg tgcgccctcg gaagtccccc ttgtccatcg
    37441 ccacggccca gccgagcccc gcctcgaacg ccgtcgatgc ctcgaagagg tcctggccgt
    37501 agagcgggta tcccatctcg agccggagga tgtcgcggga ggcgagcccg cagggagcgc
    37561 ctccgagcgg gcggaccgag ccgagcagct cgtcccagag ctgccgggcg acgtcctggt
    37621 aggtgaacag ctcgaacccc acctcgccgg tgtagccgga ccgcgtcacg atcaccggcc
    37681 ggcggtggta ctcggtctcc atgcatcgca tgaaggcgag atcgggagcc gtcgggaacg
    37741 tcgcggcgat cgcgcccatg gcttcgggac cctggaccgc gaggaagcac cagtcctggt
    37801 ggtacatcag gtgcaggggc tcgtcggcct tctcctcttc gagcatccgg aggacgcggg
    37861 gggcgttgga ggcgttcgga accacgaaat agcgctcctc accgaggcga tagacgatga
    37921 ggtcctcgat caccc
//
LOCUS       DASUYD010000126         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1170572, whole genome shotgun sequence.
ACCESSION   DASUYD010000126 DASUYD010000000
VERSION     DASUYD010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170572"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF195_06990"
     CDS             complement(<1..251)
                     /locus_tag="VF195_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6580599.1"
                     /translation="MLACRNCGRESPDDFAFCPACASPLLAGPLEREVRKTVTVVFCD
                     VTGSTALGERLDPESLRDVQSRYFEAMRRAIERHGGTVE"
     gene            complement(647..880)
                     /locus_tag="VF195_06995"
     CDS             complement(647..880)
                     /locus_tag="VF195_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580600.1"
                     /translation="MAAGQLERDSRLMIAVLVALNALAFAAVFDLGGVLRVLATLSLL
                     VVLVAAAMNGGRGSPQRSTFKAWRRLFVATGNL"
     gene            1163..1531
                     /locus_tag="VF195_07000"
     CDS             1163..1531
                     /locus_tag="VF195_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580601.1"
                     /translation="MGRPITHFEIASPNLESATEFYRQLFDWTVGEELIDGYRLVQTA
                     EGSIGGGLLRAPHGVFPYVTIYVSVDDLRASLEKAEELGGKMVVEPMPIPGVGTFAMF
                     QDPDGVMIGLFEEKVPQAQP"
     gene            1578..2111
                     /locus_tag="VF195_07005"
     CDS             1578..2111
                     /locus_tag="VF195_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014815337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6580602.1"
                     /translation="MPPHCDSLDGPVVRAAMKALDEGDVDLVLPFVKVDGEDAIRAAF
                     LRSMAARAGGPEAREVADLYFFETTVRVHRRGENAPYTGLKPAGLDVGPVIPVAEEAI
                     ETGDPGGLVRLLSDLVHDEVKRRLDHVLALRPAPGASVPEVREYVEAMLGLQVWSNMV
                     FKTLQGDPHAQSGAHED"
     gene            complement(2455..4092)
                     /locus_tag="VF195_07010"
     CDS             complement(2455..4092)
                     /locus_tag="VF195_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580603.1"
                     /translation="MAHKVEGGSAAPGISPRRRRPVGEPPPLPRDLGGTATLWILTLL
                     LLGAIYVWGKYGGPTGGQFQRWLDRTITSAFPDLSNGRLSTTGRFLAGNVGLWLLIAI
                     RWGTAVALIAYRRWRHLAVFVGSVVLVTVSVRWFPTAGIAESGITKHPAYAAASLAVT
                     VMGLVYGLVPAGRPRRRALAIGVTSCAVLAVGSILTRTYTPSEIGIGFVIGFSIPFLG
                     FRVFAPEAVFPVTYRSGRTAHLDIGGDRGPAIDIALREQLGLEVAHKEPFGLAGSGGS
                     TPLKIELTDGRRLFGKLYAQSHLRADRWYKLGRTVMYGALEDERSFNSVRRMVEYEDY
                     MLRYLRDCHVPTAEAYGFAEITPEREYLLVTGFIDDAVEMLDAPVDDTVIASGIALIR
                     SLWDAGLAHRDIKPSNLLVGAGKIYLIDVFFCQVRPSAWRQSVDLANMLMLLALGSEV
                     DRIYRAALRWFTPDDIAEAFAATKGITVPGQLRAMVRADPRLLAKEFSRLAPDRRRIP
                     VQRWTVRRITLTVVLAGVGLVAVISAILSLRAAGFRP"
BASE COUNT          722 a         1409 c         1431 g          646 t
ORIGIN      
        1 ttctcgacgg ttccgccgtg acgctcgatc gcccggcgca tcgcctcgaa gtagcgcgat
       61 tgcacgtcgc gcagcgactc gggatccagg cgctccccga gcgccgtcga gccggtcacg
      121 tcgcagaaca cgaccgtcac cgtcttgcgg acctcgcgct cgagcgggcc ggccaacagc
      181 ggggacgcgc aggcggggca aaaggcgaag tcatcgggac tctcgcgacc acagttccgg
      241 caggccagca tcggggcgag cctacggcgg gggacatctc cgcgctagcc gtcagtctga
      301 gagacggggt gcactctgaa gcaggggtgc cgtttgtcgg ccccctatct cccaacccct
      361 cacacggagg accgcctcct cccctcaaac agggtccgcc accctctcga tccgcgcttc
      421 aggctccatt agcgatacgg ggccgggccc agcggcctga aggctaccgg ggcggctggg
      481 cgagcctcgt cgccaacctc gtacttgtgg cgctggcgat cggcatgccg atctgggagt
      541 ccctcgccgg ctgtctcaaa agcgcggcct cgacgtgcat cgggtcgccg gcccccgtgt
      601 ctcgcctggt cactgagcgc accgggagcc cgcgccaggc gtcccctcat agattccccg
      661 tcgcgacaaa cagccgtcgc caggccttga acgtgcttcg ttgggggctt cctcttcctc
      721 cgttcatcgc cgctgcaacg agcacaacca acaggctgag agtcgccaag accctcagaa
      781 cgccgccgag atcgaacaca gcggcaaagg ccagggcgtt cagggctacg agaacagcga
      841 tcatcagccg actgtccctc tcgagctgtc ctgcagccat cgtcctctcc tttccatctc
      901 tacgttcggc acgctttgcc tctacccaga ccttgcgccg aatgccacct cttggcccgg
      961 gtcggtcggc cctgcgctga tagggcctat gccgaaacgc cttgcgacca cgcttgggct
     1021 tggctcgctg gcagatcgcc gccaggatcg tgtgtctctt tgggggatgt ccgaaggtcg
     1081 cgcggatcag gggacgttcg accccctgct acgggtcgac ggggcgagag cattgcatcg
     1141 aagccaattg aggagggact cgatgggacg gccgatcacg cacttcgaga tcgcatcacc
     1201 gaacctcgag tcggcaaccg agttctatcg gcagctcttc gactggactg tgggtgagga
     1261 gctgatcgat ggctaccggc tggtccagac ggccgagggg agcatcgggg gcggcctgct
     1321 gcgggcgccc cacggcgtct tcccgtacgt gaccatctac gtgagcgtcg acgatctccg
     1381 ggcgagtctt gagaaggcgg aggagctcgg cgggaagatg gtcgtcgagc cgatgccgat
     1441 tcccggcgtc ggcacattcg cgatgttcca ggaccccgac ggggtgatga tcgggctctt
     1501 cgaggagaag gtgccgcaag cgcaaccatg acgcgagcga tacgcgaagt ccaccggcta
     1561 cgaggaggtg tcgggcgatg ccaccgcact gcgattcctt ggacggaccc gttgtcagag
     1621 ccgccatgaa ggccctcgac gagggggacg tcgacctcgt ccttccattc gtgaaggtgg
     1681 acggcgagga cgcgatccgc gctgcattcc tgcggtctat ggccgcacga gcaggcggtc
     1741 cggaggcacg tgaggtcgcc gacctgtact tcttcgagac cacggtgcgg gtccatcgcc
     1801 gcggcgagaa cgccccgtac accgggctca agcccgccgg actcgacgtg ggacccgtca
     1861 tcccggtggc ggaggaggcc atcgaaacgg gcgacccggg gggcctcgtt cggctgctct
     1921 ccgacctcgt ccacgacgag gtcaagcgcc gcctggatca cgtcctggcg ctgcgccccg
     1981 cccccggggc gagcgtgcct gaggtcagag agtacgtcga ggccatgctg ggattgcagg
     2041 tgtggtccaa catggttttc aagacgctcc agggcgatcc gcatgctcaa agcggggcgc
     2101 acgaagactg aggaaggtcc tcgacgggtg agacggccag ggcggcgggc cgcccggagc
     2161 tcggcgtcgc tcaggccgcg gtcgcgagga cctgctcgat cacgacgcgt gcagccttga
     2221 gctcggcgag ccggcgtcgg ggatcgttga aaccgatgac gcgaagcgct cgatctggcc
     2281 gcgagaggcg gtcgcggaac gtcagcgttc gccagagcgg gtcgcggacc gacggaactc
     2341 gcggtgcccc atcacccgca tgaagtcctc gcggcgtagc tggcaggtgc catgtgcatg
     2401 agcgcctcca cctggagatc ccgaaggtcc gtgggaggcg tggcctgatg cttctcacgg
     2461 tcgaaagcct gcggccctca ggctcaagat cgcggagatc accgcgacca ggccgacacc
     2521 agccaggacg acggtcaacg tgatgcgacg gaccgtccac cgctgcacgg ggatccgacg
     2581 gcgatccggc gcaagccggc tgaactcctt cgcaagaagc cgtgggtcgg ccctcaccat
     2641 cgcgcgcagc tgacctggga ccgtgatccc tttggtggcg gcgaaggcct ccgcgatgtc
     2701 gtccggcgtg aaccagcgga gcgccgcacg gtagatgcga tcgacctccg acccgagcgc
     2761 gaggagcatc agcatgttcg ccagatcgac ggactggcgc cacgccgacg gtcgaacctg
     2821 gcagaagaag acgtcgatca ggtagatctt gcccgctccg acgagcaggt tggagggctt
     2881 gatgtcgcgg tgggccaggc cggcgtccca gagcgatcgg atcagcgcga tgccgctggc
     2941 gatcaccgtg tcgtcgacgg gcgcgtcgag catctccacg gcatcgtcga tgaagcccgt
     3001 gaccaacagg tattcgcgtt cgggcgtgat ctcggcgaag ccgtacgcct cggcggtcgg
     3061 gacgtggcag tcgcggaggt accggagcat gtagtcctcg tactccacca tccggcggac
     3121 ggagttgaac gagcgttcgt cctcgagcgc tccgtacatc acggtacgcc cgagcttgta
     3181 ccagcggtcg gcgcggagat ggctctgcgc gtacagcttc ccgaagagcc gacgcccgtc
     3241 ggtgagttcg atcttgagcg gcgtcgagcc gcccgaaccg gcgagcccga acggttcctt
     3301 gtgcgcgacc tcgagcccca gctgttcccg gagcgcgata tcgatcgcgg ggccgcggtc
     3361 cccaccgatg tccaggtgcg cggtccgtcc cgatcgatag gtcacgggga agaccgcttc
     3421 gggagcgaag acacggaagc ccaggaaggg gatcgagaat ccgatgacga atccgatccc
     3481 gatctccgac ggggtatacg ttcgggtcag gatcgagccg accgcaagga ccgcgcacga
     3541 agtcaccccg atcgcgagag cccgtcgacg cggccgcccg gcggggacga gtccgtacac
     3601 gagacccatg acggtgacgg cgagggaggc agccgcgtac gccggatgct tcgtgatgcc
     3661 cgactcagcg atgcccgcgg tcgggaacca tcggacgctc accgtcacga gcaccaccga
     3721 acccacgaag acagcgaggt gccgccagcg cctgtatgcg atcaacgcca ccgcggtccc
     3781 ccaccggatc gcgatcagga gccagaggcc gacgttgcct gcaaggaacc tgccggtcgt
     3841 cgaaagccgg ccgttgctga ggtcggggaa ggcggaggtg atggtgcggt cgagccagcg
     3901 ttggaactga ccgcccgtcg gtccgccgta cttcccccag acgtagatcg cgccgagcag
     3961 caagagcgtc aggatccaca gcgtggcggt gccgccgaga tcgcgtggca gagggggcgg
     4021 ttctccaacc ggccgccgcc gacgcgggct gatacctggg gcggccgacc ctccctcgac
     4081 cttgtgggcc atccggtcct ccggcgtgat gctacgccgc tctggtggag tgggccatgt
     4141 cggtcgccat cccgagctgc cctcagagcg tccgcacgac tcggcacggg gaacccaccg
     4201 ccaccacc
//
LOCUS       DASUYD010000127         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1832055, whole genome shotgun sequence.
ACCESSION   DASUYD010000127 DASUYD010000000
VERSION     DASUYD010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832055"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1473
                     /locus_tag="VF195_07015"
     CDS             <1..1473
                     /locus_tag="VF195_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX6580604.1"
                     /translation="VVSVSADSEMQPQDAAYDPATDVDSMNAVTRYTGAVDWWNTGYT
                     GAGVDVAVIDTGVSPVEGLATPGKVLNGPDLSIESQAPNLRYFDTFGHGTFMAGLIAG
                     HDSELTDPYADAPASSYRGMAPDARIVSVKVGTADGGTDVSQVIAAIDWVVQHAHDPG
                     LNIRILNLSYGTNSTQSYTIDPLAFAAEQAWKHGIVVVAASGNSGFQRHMNNAPALAN
                     PAYDPFLLAVGASDSQGTGTLADDLVPAFSPWVNRGATRGVDLVAPGTHLQGLRVPNS
                     FIDTNHPEGLLGDRYFRGSGTSQAAAIMSGAAALVLQKYPSATPNQVKQLLTSTAYPI
                     SGKSQAIGGGELQLSAALTAPLPTFTQWWAPSTGVGSLELARGEDHISMDGVVLDGER
                     DIMGSPFNAAEMALLEAQAKSWSGGDWNAKTWSGDAWSGDAWEAKTWSGDSWSAKTWS
                     SCVFSSNSWEAKTWSAKTWSGDSWSGASWSGGVWATESWS"
     gene            1483..3795
                     /locus_tag="VF195_07020"
     CDS             1483..3795
                     /locus_tag="VF195_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580605.1"
                     /translation="MSSFRELPLSARVVVVGYVALGAFGVLLRVPEMRTWSWEDAAAT
                     VGLALAAALSEQFTVAMTHRTEVENFSVTDAVWVPALILVPPSVLTLSVLLGSLAGHA
                     YRRWAWYKVAFNASQFVVAITVAEFVYRLFDLPSSFSFMTCVACAVAMSFYFAINEIS
                     VALIISRVEGIPLREVVVLPGGLNLLHAGGNLTIGMLAALVWHAGPMGLPLLIAPVVL
                     SFFAYRGWLQNKREEEQRREGDRMRTLYEAGRSLVGPLDANFDFQPFLGFVQRLVDAA
                     GTELAVRDEAGLRVYNSESGLYLTVQTEMGPADPTAYVSARPGLATYVAAVRDEANEV
                     GTLAVHRATELSPSERALVDSLASQVSARQRNQQLFDETVEQRGHLADVVGSSSDGIF
                     VVSGAGTVLSWNPAMERITGYLADEVVGRPSGDVLRIPGGPRVDGSPPTELALGVEEP
                     QDALILRRDGSDRWIRYSSSTMPERQGSGTSLVVTARDVTAELEAEQMKRDFVATVSH
                     ELRTPLTPLKGLLQSLNKGLVEDSPQARHEYHAIMLRQTERLERLISDLLDVSRFDAG
                     HLPMDAVPVEVGALLEREIADASQVTAVREVRFKRPERPVWVVADPLRLGQIVTNLLS
                     NAFKYSPEGVPVIVGLTKLGDFALVSVQDRGEGIAIDDQDRVFERFYRVDNGLTQIAG
                     GFGLGLFIARRLAEAMGGGLVLSSRPGEGSTFSVSLPLLTAAPEADDLGDQRLSLRDS
                     DTDHHEPDADQPNGHVLTDADSRVLVQRSE"
     gene            3996..>4304
                     /locus_tag="VF195_07025"
     CDS             3996..>4304
                     /locus_tag="VF195_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580606.1"
                     /translation="MAGDVIGRESELAILHRFLDSIPTGPSALLLSGDPGIGKTTVWK
                     RGLAGALECGFRTLSCNPVEAETRLSYAALGDLVEPVLDESLPTLPEPQREALEVALL
                     R"
BASE COUNT          679 a         1440 c         1469 g          716 t
ORIGIN      
        1 gtcgtctccg tcagcgcgga ctcggagatg cagccccagg acgccgcgta cgatccggcg
       61 accgacgtcg actcgatgaa cgccgtgacc cgctacacgg gcgcggtcga ctggtggaac
      121 accggctaca cgggagccgg cgtcgatgtg gcggtgatcg acaccggcgt gtccccggtc
      181 gagggcctcg caacgcccgg caaggttctc aacgggccgg acctctcgat cgagtcccag
      241 gcgccgaacc tcaggtactt tgacacgttc ggccacggga cgttcatggc cggactcatc
      301 gccgggcacg attcggaact caccgatccc tacgcggacg ctccggcttc gtcctatcgg
      361 ggcatggccc ccgatgcccg gatcgtcagc gtcaaggtcg gcacggcgga cggcggcacc
      421 gatgtctccc aggtgatcgc cgccatcgat tgggtcgtcc agcacgcgca tgaccccggc
      481 ttgaacatcc ggatcctgaa cctctcctac ggaaccaaca gcacgcagtc gtacaccatc
      541 gatccgcttg ccttcgcggc cgagcaggca tggaagcacg gcatcgtcgt cgtcgcggcg
      601 agtggcaaca gcggcttcca gcgccacatg aacaacgcgc ccgcgctcgc caaccccgcg
      661 tacgacccat tcctgctcgc cgtcggtgcg tcggactcgc agggaacggg cacgctcgcc
      721 gacgatctcg tgccggcgtt ctcaccgtgg gtcaatcgcg gcgcgacgag gggggtcgac
      781 ctggtcgcgc ccggaaccca tctgcagggg ctgcgcgtgc cgaactcatt catcgacacg
      841 aaccatcccg aaggactcct cggcgaccgg tacttccggg ggagcgggac gtcgcaagcg
      901 gcggcgatca tgtcgggggc ggcagcgctc gtgctccaga agtacccgtc cgcgaccccg
      961 aatcaggtga agcagctgct cacctccacc gcgtatccga tcagcgggaa gtctcaggcc
     1021 atcggcggcg gcgagctcca gctctcggcc gccctgaccg cgccgcttcc gacgttcacg
     1081 cagtggtggg ccccctcgac gggagtgggc tcgctggaac tcgcccgcgg cgaggaccac
     1141 atctcgatgg acggcgtcgt gctggacggc gagcgggaca tcatgggttc gccgttcaac
     1201 gccgccgaga tggccctgct cgaggctcag gcgaagtcct ggtcgggcgg cgactggaac
     1261 gccaagacct ggagcgggga cgcttggagc ggggacgcgt gggaggccaa gacctggagc
     1321 ggggattcct ggagcgccaa gacgtggtcc tcctgcgtct tctcgagcaa ctcctgggag
     1381 gccaagacct ggagcgcaaa gacctggagc ggcgactcct ggagtggagc ctcgtggagc
     1441 ggcggcgtat gggccacgga gtcctggagc tgatcgagtg agatgagttc cttccgcgag
     1501 ctgccgctct ccgcccgagt ggtcgtcgtc ggctacgtgg cgctgggagc gttcggcgtc
     1561 ctcctgcggg tcccggagat gcggacctgg tcctgggagg acgccgccgc gaccgtgggg
     1621 ctcgcgctcg cggcggcgct gagcgagcag ttcacggtcg cgatgacgca ccgtacggag
     1681 gtcgagaact tctcggtcac ggatgcggtg tgggttccgg cgctcatctt ggtcccccca
     1741 agcgtgctca cgctcagcgt gctcctgggg agcctggcgg gacatgcata ccggcgctgg
     1801 gcctggtaca aggtcgcctt caacgcgagc cagttcgtcg tcgccatcac cgtggcggag
     1861 ttcgtctacc ggctgttcga tcttccatcg tcgttcagct tcatgacctg cgtggcgtgc
     1921 gccgtcgcca tgtcgttcta cttcgccatc aatgagatct cggttgcgct gatcatctcg
     1981 cgcgtggagg ggatccctct ccgcgaggtc gtggtcctcc cggggggtct gaacctcctc
     2041 catgccgggg ggaacctcac gatcggcatg ctcgccgccc tcgtgtggca cgcggggccg
     2101 atgggcctcc ccctgctgat cgcacccgtt gtcctatcct tcttcgccta ccgaggttgg
     2161 ctgcagaaca agcgggaaga ggagcagcga cgggagggtg accgtatgcg gaccctctac
     2221 gaggccggaa ggtccctcgt cggtccgctc gacgcgaact tcgacttcca gccgttcctc
     2281 gggttcgtcc agcgcctcgt tgacgcggcg gggaccgaac tggccgtgcg ggacgaagcg
     2341 gggctgcgcg tctacaactc cgagagcggc ctgtacctga ccgtccagac cgagatgggt
     2401 ccggcggacc cgacggcgta cgtgagcgcc cgcccggggc tcgccacgta cgtcgccgcc
     2461 gtccgcgacg aggcgaacga ggtcgggacc ctcgccgtcc accgcgccac cgagctatct
     2521 ccgtcggaac gcgcgctggt cgactcgttg gcttcccagg tctccgcgag gcaacggaac
     2581 cagcagctct tcgacgagac ggtcgagcaa cgtggccacc tggcggacgt ggtcggcagc
     2641 agctcggacg gcatcttcgt cgtctccgga gcgggcacgg tgctctcgtg gaacccggcg
     2701 atggagagga tcaccggcta cctcgcggac gaggtggtcg gtcgtccttc cggcgacgtt
     2761 ctccggatcc ccggcgggcc gagagtggat ggatcgcccc cgacggagct cgcgctcggg
     2821 gtcgaggagc cccaggacgc gctgatcctg cggcgcgacg gatcggatcg gtggatccgc
     2881 tactcgagca gcacgatgcc cgagcgacag ggaagcggaa cgagtctcgt cgtgaccgct
     2941 cgcgacgtga cggccgagct cgaggccgaa cagatgaaac gcgacttcgt cgccaccgtg
     3001 tcccacgagc tcaggacgcc gctcacgccc ctcaagggct tgctccagtc gctgaacaag
     3061 gggctggtcg aggactcgcc tcaggcacgc cacgagtacc acgcgatcat gctgcgccag
     3121 accgaacggc tggaacggct catctccgat ctcctcgatg tctcacgctt cgatgccgga
     3181 cacctgccga tggacgcggt tccggtggag gtcggtgcgc tcttggagcg cgagatcgcg
     3241 gacgcgagcc aggtgacggc agtccgcgag gttcggttca agcgtccgga acgtccggtt
     3301 tgggtcgtcg ccgaccccct ccgcctcggc cagatcgtca ccaacctgct gtcgaatgcg
     3361 ttcaagtact cgccggaggg agtcccggtg atcgtggggc tgaccaagct cggagacttc
     3421 gccctggtct cggtgcagga ccggggcgag gggatcgcga tcgacgacca ggatcgcgtg
     3481 ttcgagcgct tctaccgagt ggacaacgga ttgactcaga tcgcaggtgg tttcggcctg
     3541 gggctgttca tagcccgccg attggcagaa gcgatgggtg ggggtctggt tctgagctcg
     3601 cggccgggag aaggatccac gttctcggtc agcctgccgc tcctcacggc agcgccggag
     3661 gcggacgatc tgggggacca acgcctttcc ttgcgtgact cggacaccga tcatcacgaa
     3721 ccagatgccg atcaaccgaa cggccatgtg ctcacggacg ccgattcccg ggtactggtg
     3781 cagcgctcag aataggagct gcctgttcct tcgctgctcg gtttcgcgag tcaggtcgcg
     3841 gcaagcggaa cgatcccccg gtgaccacgc agctctgtca gggtgacccg gtacacattg
     3901 gtacacggcc gacaacggcc gggccgcgac aggtttgcaa gcttgggatc cgccatctct
     3961 agaatcctgg gtccactgaa gcggggagag cgtctttggc cggtgacgtc atcgggcgcg
     4021 agtccgagct cgcgatcctg catcgcttcc tggactcgat cccgaccggc ccgagcgcct
     4081 tgctcctgtc gggtgatccc ggcatcggga agacgaccgt ttggaagcgc gggctggcgg
     4141 gagccctcga gtgcggcttc cggacgctct cgtgcaaccc cgtcgaggcc gagacgcgtc
     4201 tgtcgtacgc cgccctcgga gacctcgtcg agcccgtcct ggacgagagc ctgccgacgc
     4261 tccccgagcc gcaacgcgag gccctggagg tggcgctcct gcga
//
LOCUS       DASUYD010000128         7886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1832238, whole genome shotgun sequence.
ACCESSION   DASUYD010000128 DASUYD010000000
VERSION     DASUYD010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832238"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VF195_07030"
     CDS             <1..260
                     /locus_tag="VF195_07030"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030677 - ribonuclease P complex
                     [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_function: GO:0042781 - 3'-tRNA processing
                     endoribonuclease activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX6580607.1"
                     /translation="HGKRVVLFVAPGSGEFALIAGKKIGGAVQRNRARRVLRAAIREV
                     APRGVEGHDVVLVAREAIRDARTQDLITEMTDLLHRGGTRR"
     gene            257..592
                     /gene="yidD"
                     /locus_tag="VF195_07035"
     CDS             257..592
                     /gene="yidD"
                     /locus_tag="VF195_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX6580608.1"
                     /translation="MTSIRRVLWNVGAPARLALLAAIRTYQLTLSGWLGGQCRFSPTC
                     SRYAEEAIRSLGVMRGSLLATRRVLRCNPFGRGGLDPVPQPKTSYDTNIRPEAESTLP
                     YLIRRRHAG"
     gene            582..1730
                     /locus_tag="VF195_07040"
     CDS             582..1730
                     /locus_tag="VF195_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HEX6580609.1"
                     /translation="MLAEIGPFQALLDAIGWLLAWLYDLTGSFGFAIIILTIVFRIVL
                     LPLGIKQIKSMQAMQALQPKIKEIQKKFKGNKQKVQEQTMKLYQEYGVNPLGGCLPLL
                     LQFPILIAMYSVIRAPVPLPSSPDSPGVVMYKDNHLPEDSALFADITAHEGKGQGFLL
                     MNLQCSPQNAGKTVDVRDTAGDPTEDNLECGNGIADRIPYYAILILMIGTTFYSSRQT
                     QKATPASAQNQQTQIITKVMPLMFGVFGFAFPAGLILYWTVSNLFQIGQQAVMLRLGH
                     IGPQAMDRRLTEARSKAETRTDKPRTGIMGRMMERAEQERKRRLGEPEPPRKPPPRTS
                     GSSKASPKGGSGRGTGKGSGSSSSGDRSKGGSAGPRRSGPNRPKRRGR"
     gene            1734..2264
                     /locus_tag="VF195_07045"
     CDS             1734..2264
                     /locus_tag="VF195_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HEX6580610.1"
                     /translation="MEREPGSTGAVGEDRRDGDPDAPELEEQADAIADFVEELLERMG
                     IDAIAEPTTHHGHVYVDIVDGPEDDMALLIGRHGQTLDAIQELARTSVGRRLDDRIRV
                     LVDVGDYRKRAEERLVEQAREIAERVQRTGEEEHLEPMNAYERKLVHDVIAEFDTLES
                     ASEGADPDRFVVVRPL"
     gene            2287..2883
                     /locus_tag="VF195_07050"
     CDS             2287..2883
                     /locus_tag="VF195_07050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmG family class I SAM-dependent
                     methyltransferase"
                     /protein_id="HEX6580611.1"
                     /translation="MFHVKHEGWTPADLSPSQIASLEAFEGLLATHAIPRGMVSSSDR
                     AHLRERHVLDSLRAVPHLGPMKQPVVDLGSGAGLPGIPVALARPDLLVTLAEPRQLRV
                     AFLELVVERLSITNVRVFPRPAEELPADAGVCLARGFGDPSRTWAVARGLLHPDGSLI
                     YWAGTTFRTDQVPEGVRIAAIGSPTLESGGPIVIMTRQ"
     gene            2973..3743
                     /locus_tag="VF195_07055"
                     /pseudo
     CDS             2973..3743
                     /locus_tag="VF195_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018702047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4364..5254
                     /locus_tag="VF195_07060"
     CDS             4364..5254
                     /locus_tag="VF195_07060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013875635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX6580612.1"
                     /translation="MSKRGGLGRGLSALIPGAPEAGEVTTGLLEVPAGAIAPNPKQPR
                     SRFDDEALQELAASIREVGILQPIVVRRAGQGYEVVAGERRLRAAKLAGLATVPVVLR
                     DSDDADLLREALIENIHREDLNPIELGEAFRQLLDELGLKQEELADRVGVSRSHIANT
                     IRLLALPFEVQQLLTDEKLSAGHARALLALGDADAITALSLRVAAEDLSVRETEEIVR
                     RFIEGPPEPAPKKAVTDAEASDPNLAEVEEILSEQLATRVQIRMGRKRGQVLIEFGSA
                     DDLERIVSEIVGSGPGLAPD"
     gene            complement(5440..6471)
                     /locus_tag="VF195_07065"
     CDS             complement(5440..6471)
                     /locus_tag="VF195_07065"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX6580613.1"
                     /translation="MRIFRTGDSGPEIADIQSRLTALGAEIDGEELVGVFGSSTVAAV
                     RWFQQDRHLQVDGLVGPDTWEQLVEAGWRLGDRTLYHRAPMFRGDDVREIQRMLNALG
                     FDSGKEDGFYGPRTDSALRLFQRNVGDEPDGIVGPHTLSVLRRMRPLDAVPSRALVRE
                     REELDASRGPIEGRIVAVDVREGAAAAGGMHQRSALALVQELRAVGADPRLLVTDDEA
                     AAPSDRARLANEAGAAASITVELTARDGEGGGGPVCSYFGSRTTHSPSGLVLAQLILE
                     ELEAELGLSGRVRPLTIAPLRETRMPAVQVEPFTGAGQQEEELLRDPDLPTRLARAVA
                     VGVGRFFSG"
     gene            complement(6540..6866)
                     /gene="trxA"
                     /locus_tag="VF195_07070"
     CDS             complement(6540..6866)
                     /gene="trxA"
                     /locus_tag="VF195_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6580614.1"
                     /translation="MANIGDVNDTEFQTSVLDSETPVLVDFWAEWCVPCHMVSPVVEE
                     IGQEKGQQLKVAKLNIDENPDATRTYGVMSIPSLILFKAGKEVARVTGARPKDAILRD
                     LDPHLA"
     gene            complement(6905..>7886)
                     /gene="trxB"
                     /locus_tag="VF195_07075"
     CDS             complement(6905..>7886)
                     /gene="trxB"
                     /locus_tag="VF195_07075"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017595540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX6580615.1"
                     /translation="NDVRNMIVIGSGPAGFTAALYAARANLEPLVLKGVEAGGQLMLT
                     TDVENYPGFADGILGPELMDQMEKQAARFGAEILSVHVTRVDLSTQPYRVWAGDEVWM
                     TRTVVIATGATAKWLDVPGEETLRGRGVSACATCDGFFFRDRELVVVGGGDSAMEEAT
                     FLTKFASKVTIVHRRDEFRASKIMQDRALANPKIEVAWNAEVVEILGDDAVTGVRLRD
                     TSTGEVSELSTDGVFVAIGHTPNTELFADRLELDPDGYIVIQEPRTKTSVEGVFAAGD
                     VTDRIYRQAVTAAGQGCKAAIDAERLLEAESHAAVEAGNLAARASVQGEA"
BASE COUNT         1314 a         2672 c         2643 g         1257 t
ORIGIN      
        1 tgcatgggaa gcgggtggta ctgtttgtcg ctcccggctc gggggagttc gcgctgatcg
       61 ccggcaagaa gatcggcggc gcggtgcagc ggaaccgggc ccggcgggtc cttcgggcag
      121 cgatccgcga ggtcgcgccg cgtggggtgg aaggacatga tgtcgtcctg gtggcccgcg
      181 aggccatccg agacgcccga acccaggatc tgatcaccga gatgaccgac ctgctccatc
      241 gtggagggac gcgccgatga cgtccatccg acgcgtcctg tggaacgtcg gcgcgcccgc
      301 tcgcctggcc ctcctcgccg cgatccggac ctaccagctc acgctctcgg ggtggctcgg
      361 ggggcagtgc cggttctccc ccacctgctc gcggtacgcg gaggaggcca tccgttcgtt
      421 gggcgtgatg cgaggatcgt tgcttgcaac acgacgcgtg ttgcgttgca acccgttcgg
      481 tcgtggcggc ctcgatccgg tgccgcagcc caagacgtcg tatgacacca acatacgccc
      541 ggaggccgag tcgacgctcc cctatctgat ccggcggcgc catgctggct gagatcggtc
      601 cgttccaagc gctactcgat gcgatcggat ggctgctcgc gtggctgtac gacctcaccg
      661 gcagcttcgg cttcgcgatc atcatcctga ccatcgtctt tcgcatcgtc cttctccccc
      721 tgggcatcaa gcagatcaag tcgatgcagg cgatgcaggc gctccagccc aagatcaagg
      781 agatccagaa gaagttcaag gggaacaagc agaaggtcca ggagcagacg atgaagttgt
      841 atcaggagta cggcgtcaat ccgctcggcg ggtgcctccc cctcttgctt cagttcccga
      901 tcctgatcgc gatgtattcc gtcatcaggg cacccgtgcc cttgccctcg agcccggact
      961 ctccgggggt ggtcatgtac aaggacaacc accttccgga ggactcggcg ttgttcgccg
     1021 acatcacggc ccacgaaggg aaggggcagg gcttcctctt gatgaacctc caatgctcgc
     1081 cgcagaacgc gggcaagacc gtggacgtcc gagacaccgc cggcgacccc acggaggaca
     1141 acctggagtg cgggaacggg atcgccgacc gtatccccta ctacgcgatc ttgatcctga
     1201 tgatcgggac cacgttctac tccagccgac agacgcagaa ggcgaccccg gcgtcggcgc
     1261 agaaccagca gacccagatc atcacgaagg tcatgccgct catgttcggg gtcttcggat
     1321 tcgcgttccc tgcgggtctg atcctctatt ggacggtgtc caacctcttc cagatcggcc
     1381 aacaggccgt gatgctccgg ctcggccaca tcggtccgca ggcgatggat cgacgcctca
     1441 ccgaggcgag gtcgaaggcc gagacgcgaa ccgacaagcc gagaacgggc atcatgggca
     1501 ggatgatgga gcgggctgag caggaacgga aacggcgtct cggtgagccg gagccgcctc
     1561 ggaagccgcc tccgcgcacg tcgggctcga gtaaggcctc acccaagggt ggctctggcc
     1621 gtggaaccgg gaagggttca ggctcgagca gctcgggaga tcggtcgaag ggcggctcgg
     1681 cgggtccgag gcggtccgga ccgaatcggc cgaagcggcg cgggaggtag acgatggagc
     1741 gagagccggg atcgacgggt gccgtgggcg aggatcgccg agacggggat ccggatgctc
     1801 ccgagctcga ggagcaggcc gatgcgatcg ccgacttcgt cgaggagctc ctggagcgga
     1861 tggggatcga cgccatcgcg gaacccacga cccaccatgg ccacgtctac gtcgacatcg
     1921 tcgacggccc agaggatgac atggcgctcc tgatcgggcg gcatggccag acgctcgatg
     1981 cgatccagga gctggcacgt acatcggtgg gccgccgttt ggacgaccgc atccgcgtgc
     2041 tggtcgatgt cggggactac cggaagaggg cggaggagcg cctggtcgag caggcgagag
     2101 agatcgccga gcgggtgcaa cgtacgggcg aggaggagca cctcgagccc atgaacgcct
     2161 acgagcgcaa gctggtgcac gacgtgatcg cggagttcga taccctcgag agcgcatccg
     2221 agggtgccga tcccgatcgg ttcgtggtgg tgcggcccct ctgaggagcc ctccgtcagg
     2281 gaggccatgt ttcacgtgaa acatgagggt tggacccctg ccgatctatc ccctagtcag
     2341 atagcttctc tcgaggcgtt cgaaggtctc ctcgccacac acgcgatccc tcgcggcatg
     2401 gtgtcatcgt ccgacagggc tcacctacgc gagcggcacg tgctcgacag cctgcgggcc
     2461 gtccctcacc tcggaccgat gaagcagcct gtggtcgacc tcggatcggg agcagggctt
     2521 cccggtatcc ccgtcgcgct agctcggccg gatctgctcg tcacgctcgc cgagccccgg
     2581 cagttgagag tcgccttcct cgagctcgtc gtcgagcgtc tgtcgatcac gaacgtccgc
     2641 gtcttcccca ggcccgccga ggagctgccc gcggatgccg gggtatgcct cgccaggggg
     2701 ttcggcgacc cctctcggac ctgggcggtc gcccgggggc ttctccaccc cgacgggagc
     2761 ctgatctatt gggcaggaac cacgttccgg acggatcagg tgcccgaagg cgttcgcatc
     2821 gcggccatcg ggagccccac ccttgaaagc ggcggaccca tcgttatcat gacccgacag
     2881 tgagcaaggc gcctgccagc tcgtcccacg ccgtggcacc caccacgccg actcgcccct
     2941 cctcgcccaa gcggtcgagg aagaaggggc ccgtggttcc cgacgtccct ggccgagcca
     3001 gggtcatcgc gatcgcgaat cagaagggcg gggttggcaa gagcacgacg gcggtgagtc
     3061 tcggcgcggc gctggccgac atcgggtatc gcgttctggt ggcggatctc gacccgcagg
     3121 ggaacgcgtc cacggggatg gggatccggc acgacgctcg cgaggtcacg gtgtatgaca
     3181 tcgtcgtctc cgaggccccg atccaggagg cgatcgtaca gacccccgtg gagcggctcc
     3241 atgcggtgcc ggcaacgatc gacctggccg gagccgagat cgagttggtt tctcaattct
     3301 cgcgcgagtc gaggctgaag aaggcgatcg aacccgtcct cgagggagcg tatgacttca
     3361 tcctcctcga ctgtcccccg tccctcgggc tgctcacgat caacgctctc accgcggccg
     3421 aggagctgat cgttcccatc cagtgcgagt actacgccct ggaggggctg ggccagctgc
     3481 tccggaacgt ctccctcgtc cagcagaaca tcaactcggg tctcaggctg tcgggcatcg
     3541 tgatgacgat gttcgacccg cggaccaagc tgtcggagca ggtcgtggca gaggtcaagc
     3601 ggcatttcca cgatctcgtc tacgacgtga tcatcccgcg gacggtccga ttgtccgagg
     3661 cacccgggtt cggacagccc atcacgatgt acgactccaa gtcgaagggc gcagaggcat
     3721 accgtcagct cgcgcaagag gtggcgctgc ggcctccgcc ggaggcaccc atgccgcagt
     3781 tcgacaagat gcccatcgcc gtcatgcctc cgatggagcg accgggggac gccgaggagg
     3841 cttccggtaa cggggagcat cccgacccgt cggaaccgcg agtctccgga tccgatccta
     3901 cggtcgagcg atcggccggg agcgagcctg tcgtcgaccc gacctccgag ccggttgccg
     3961 cggaaccgcg gcccgagccg gccgcacggg ccccggaacc cgagcctccc gcggaccccg
     4021 taccacacct cgagatgacc gcatctccta cctcgaggtc acgtgctgct cgaggggccg
     4081 ctggcacggc ggcggatgcg gacgatgacg tctgggagga gcccgctcgc caacctgagc
     4141 cgagggtcaa gtccccgtcg agaccccttc caccgggcca gttgccccag cgacgggtcg
     4201 tcgtgatcga cgagaactcc gatatggata tcgaggcagg tcaagaggcg cctccggctc
     4261 ccgcggacga gcatcgtgag gggatggcgg acatcggcgc gacgttgaac gaggagcagg
     4321 gtcggaaacg gcgttggcgg atgttccgca agggaggcga ttgatgtcca agcgaggggg
     4381 tctgggacgg ggactctccg cgctgatacc cggcgcgccc gaggcggggg aggtgaccac
     4441 cgggctgctc gaggtgcccg caggggcgat cgcccccaac ccgaagcagc cgaggagcag
     4501 gttcgacgac gaggcgttgc aggagctcgc cgcgtccatc cgcgaggtcg ggatccttca
     4561 gccgatcgtg gttcggcggg cgggccaggg ctacgaggtc gtcgccggtg agcggcgcct
     4621 ccgagccgcg aagctcgccg ggctggccac ggtcccggtg gtcctgcggg attccgacga
     4681 cgcagatctg ctcagagaag ccctgatcga gaacatccac cgggaagacc tcaacccgat
     4741 cgagctcggc gaggcgttca ggcagctcct ggacgagctc gggttgaagc aggaggagct
     4801 ggccgatcgc gtcggcgtct cacgatcgca catcgccaac acgatccgcc tcctcgcctt
     4861 gcccttcgag gtgcagcagc tcctcacgga cgagaagctg tcggctggtc acgcgcgagc
     4921 gctgctcgcc ttgggagatg ccgacgcgat cacggctctc tctctgcggg tcgcggcgga
     4981 ggatctctcg gtccgcgaga cggaggagat cgttcgacgg ttcatcgagg gtccgcccga
     5041 gcccgctccc aagaaggccg tcacggatgc ggaggcctca gaccccaacc tcgccgaggt
     5101 cgaggagatc ctgtccgagc agctcgccac ccgggtgcag atccgaatgg gccgcaaacg
     5161 gggtcaggtc ttgatcgagt tcggttccgc cgacgacctc gagcggatcg tgtcggagat
     5221 cgtcggttcg gggcccggcc tcgccccaga ctgagtgata tcatcataca ccccccggcg
     5281 aatcgtgcgg cctcctcgcg ggactcacaa ccccatccgg tacgctcggc agatggatga
     5341 cgctggcccg tacccgtcgg agcgggaacg cgcgctgaag gcgaccttcc tcggtctgat
     5401 cctcggcgtg gtcctcgcga tgctcgcacg tcgccgcgcc tagcctgaga agaagcggcc
     5461 caccccgacg gcgacggcgc gggcgagacg cgtagggagg tcggggtcgc ggagaagctc
     5521 ttcctcctgc tggcccgcgc cggtgaacgg ttcgacctgg accgcgggca tccgcgtctc
     5581 gcgcagaggt gcgatcgtca ggggccggac ccgtccggac aggccgagct ccgcctcgag
     5641 ctcttccagg atcagttgcg cgagaacgag tccagaaggg gaatgggtgg tccggctgcc
     5701 gaagtacgag cagacggggc cgcccccacc ctccccgtcc ctggcggtga gctccacggt
     5761 gatggacgcc gccgcaccgg cctcgttcgc gaggcgggcg cggtccgaag gcgcggccgc
     5821 ctcgtcgtcg gtaacgagga gcctcggatc ggcacccacc gcccgcagct cctgcaccaa
     5881 ggcgagcgcc gagcgctgat gcattccgcc ggcagcggcg gccccttccc tgacgtcgac
     5941 ggcgacgatc cgcccctcga tcggccctcg ggaggcatcc aactcctcgc gttcccgaac
     6001 gagggctcgc gacgggaccg cgtccagcgg gcgcatccgt cgcagcaccg acaacgtgtg
     6061 cgggccgacg atcccgtccg gttcgtcccc gacgttgcgt tggaacagcc ggagagcgct
     6121 gtccgtccgg gggccgtaga acccgtcctc cttgccgctg tcgaagccca gcgcgttcag
     6181 catccgctgg atctcgcgca cgtcgtctcc tcggaacatc ggggcgcggt gatacagcgt
     6241 gcggtcgccc agccgccaac ccgcctcgac gagctgttcc cacgtgtccg ggccgacgag
     6301 tccgtcgacc tgcaggtgcc gatcctgctg gaaccagcgg accgcggcca cggtggacga
     6361 tccgaacacg cccaccagtt cctcgccgtc gatctcggcc ccgagcgccg tcaacctcga
     6421 ctggatgtcc gcgatctccg ggccgctgtc acccgtgcga aagatgcgca tcggttcgag
     6481 tgtgcgtggg tacgcgtcgc gacggcaact gccgacagga gcagcgtggg gcagatgtcc
     6541 tacgcgaggt gcgggtcgag gtctcgaagg atcgcgtcct tcggccgtgc gccggtgacc
     6601 cgcgcgactt ccttgcctgc cttgaacaag atcaatgagg gaatcgacat gactccgtag
     6661 gtcctggtgg cgtccgggtt ctcgtcgatg ttcagcttcg cgaccttgag ctgctggccc
     6721 ttctcctgcc cgatctcctc cacgacagga gagaccatgt gacacggcac gcaccactcc
     6781 gcccagaagt cgacgaggac cggcgtctcg gagtcgagga cgctcgtctg gaactccgtg
     6841 tcgttcacat ctccgatgtt cgccatctct gctctcctct cgaccgccgg cccagccggc
     6901 tggtctacgc ctcgccctga acgctcgctc gagcggcgag attccccgcc tcgaccgcgg
     6961 cgtgggactc cgcctcgagc agccgctcgg cgtcgatcgc tgccttgcat ccctgccccg
     7021 cggcggtgac ggcctgacga tagatccggt cagtgacgtc gccggccgcg aacacccctt
     7081 cgaccgaggt cttggtgcgc ggctcctgga tcacgatgta cccgtccggg tcgagctcca
     7141 accgatcggc gaagagctcg gtgttcggcg tgtgcccgat cgccacgaag acgccatcgg
     7201 tggagagctc actgacctca ccggtcgagg tgtctcggag ccgcacgccg gtgaccgcat
     7261 catcgccgag gatctcgacc acctcggcgt tccaggcgac ctcgatcttc gggttcgcca
     7321 gggcgcgatc ctgcatgatc ttcgacgcac ggaactcgtc gcgccgatgg acgatcgtca
     7381 ccttggaggc gaacttcgtc aggaacgtcg cctcttccat cgccgagtca cccccgccca
     7441 cgacgacgag ctctcgatcg cggaagaaga agccgtcgca ggtggcacag gcggagacgc
     7501 ctctcccgcg gagagtctcc tcgcccggca cgtcgagcca cttcgccgtc gcccccgtgg
     7561 cgatcaccac ggtccgcgtc atccagacct cgtcgcccgc ccagacccga tagggctgcg
     7621 tggagagatc gacgcgggtc acgtggaccg agaggatctc cgcgccgaac cgggcggctt
     7681 gcttctccat ctggtccatc aactccgggc ccaggatccc gtcggcgaac cccggatagt
     7741 tctcgacgtc ggtggtcaac atcagttggc cgccggcctc cacgcccttg agcaccagag
     7801 gctcgaggtt ggcccgggcg gcgtagagcg ccgccgtgaa tccggcaggg ccggacccga
     7861 tcacgatcat gttccgaacg tcgttg
//
LOCUS       DASUYD010000129         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_290500, whole genome shotgun sequence.
ACCESSION   DASUYD010000129 DASUYD010000000
VERSION     DASUYD010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290500"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /gene="rplE"
                     /locus_tag="VF195_07080"
     CDS             <1..354
                     /gene="rplE"
                     /locus_tag="VF195_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018638465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEX6580616.1"
                     /translation="KARKSIAGFKLREGMAIGAKVTLRGAYMWEFVDRLVSLALPRIR
                     DFRGLSPAAFDGHGNYTLGVTEQLIFPEIDYDKVVKVTGMDITIVTTATTDDEGRALL
                     AALGFPFAQTAAEAS"
     gene            392..577
                     /locus_tag="VF195_07085"
     CDS             392..577
                     /locus_tag="VF195_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEX6580617.1"
                     /translation="MAKTALINKQKKQPKFRVRGYTRCQRCGRSRAVYRKFMLCRICF
                     RELAHLGELPGVTKASW"
     gene            577..996
                     /gene="rpsH"
                     /locus_tag="VF195_07090"
     CDS             577..996
                     /gene="rpsH"
                     /locus_tag="VF195_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX6580618.1"
                     /translation="MTKAKFSSNVSDPVADLLTRIRNANLAFKDETVAPVSKLADSVL
                     KILDAEGYIIGYSREGEGVHQAFRIRLKYGVRRRRAITGLRRISKPGRRIYRGRDELP
                     RVQGGLGIAIISTSHGVMTDKDAARKGVGGEVLAYVW"
     gene            1034..1585
                     /gene="rplF"
                     /locus_tag="VF195_07095"
     CDS             1034..1585
                     /gene="rplF"
                     /locus_tag="VF195_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX6580619.1"
                     /translation="MSRIGRQPVEIPEGVEVRVDEANQVTVRGPRGELSQTMHPTMRI
                     VTDGGVARVERPDDVGFSRSLHGLTRSLLANMVEGVTKGFEKRLQIVGVGYRAALKGK
                     DVELQVGYSHPVLVPQPDGIEFEVPAPTQVVVRGNDKQQVGEVAATIRKIRKPEPYKG
                     KGIRYENEYVRKKAGKAAKGATA"
     gene            1585..1935
                     /gene="rplR"
                     /locus_tag="VF195_07100"
     CDS             1585..1935
                     /gene="rplR"
                     /locus_tag="VF195_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016664043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX6580620.1"
                     /translation="MDAKTRRVARVRRHSRVRKKVAGTAERPRLAVYRSNRHIYVQLI
                     DDRAAATLAAASDTEVAVSKGKTDRAREVGKLIADRAKGAGIDRVVFDRGGRRYHGRV
                     AAVAEGAREGGLQI"
     gene            1935..2537
                     /gene="rpsE"
                     /locus_tag="VF195_07105"
     CDS             1935..2537
                     /gene="rpsE"
                     /locus_tag="VF195_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012392951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX6580621.1"
                     /translation="MSRRYDSRDQEKSPYEERVIFINRVAKVVKGGRRFSFAALIVVG
                     DGAGRVGVGYGKAKEVPAAIAKGVELARRKMFEVPMMGTTIPHEIVGQAGAGRVLLKP
                     AAPGTGVIAGGPVRALVEAAGIKDILSKSMGSSNQINIVKAAMAGLKALRKPHEVAMM
                     RDREDHQVAPKPMLEAVAAAEAHAQAARAEALTEVYGDKA"
     gene            2524..2745
                     /gene="rpmD"
                     /locus_tag="VF195_07110"
     CDS             2524..2745
                     /gene="rpmD"
                     /locus_tag="VF195_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX6580622.1"
                     /translation="MTRLKVTQTRSVIRRPKDQKDTVRRLGLHRIRDSVIKEDRPDIR
                     GMLVKVRHLVRVEELAGDDPKDRVEKGKT"
     gene            2746..3180
                     /gene="rplO"
                     /locus_tag="VF195_07115"
     CDS             2746..3180
                     /gene="rplO"
                     /locus_tag="VF195_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX6580623.1"
                     /translation="MKLHHLRPAKGAKKERTRVGRGRAGARGKTAGRGTKGTGARGTI
                     RAGFEGGQMPLIRRVPKLKGFKNPNRVEYAPVNVEVLGRYFDGDVTPDALYRHGLAHK
                     GAKIKVLARGELDKKLTVKAHAFSKAAKEKIEAAGGTAEVLS"
     gene            3197..>4098
                     /gene="secY"
                     /locus_tag="VF195_07120"
     CDS             3197..>4098
                     /gene="secY"
                     /locus_tag="VF195_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX6580624.1"
                     /translation="MLKAFLNAFRVPDLRAKILFTLFVIAVFRLGSYVPVPVVDIGIL
                     QSQADAGGFLNIINLFSGGALTRLSIFSLGIMPYITSSIIMQLMTVVIPKLQQWQEQG
                     ETGTKKINQWTRYVTVVLALLQSTGLVFLFHNPDASGFADVFPQGTFTGANVAFIVLI
                     MTAGTAFIMWLGELITQRGVGNGMSILIFTSVISTLPAEGNAILQQGGTGKFIAILLI
                     GLGIIVAVIYVDQGQRRVPVQYAKRVVGRKMTSGGSTYLPLKVNQAGVIPIIFATSVL
                     YFPALLSSVYHAAWFQNFVNNHVQ"
BASE COUNT          763 a         1271 c         1468 g          596 t
ORIGIN      
        1 aaggcgcgga agtcgatcgc cggcttcaag ctccgcgagg ggatggcgat cggcgcgaag
       61 gtgaccctcc ggggggccta catgtgggag ttcgtcgatc gtctcgtgtc gctcgcgctc
      121 cccaggatcc gtgacttccg cgggctgagc ccggccgcct tcgacggcca cgggaactac
      181 acgctcggtg tcaccgagca gctgatcttc ccggagatcg attatgacaa ggtggtgaag
      241 gtcacgggca tggacatcac gatcgtgacc accgccacga ccgacgacga aggacgagcg
      301 ctgctcgccg ccctgggatt cccattcgcc cagacggcgg cggaggcgag ctgaggcccc
      361 aaggagacac acaaggagac ggcaaagaga gatggcgaag accgcactca tcaacaagca
      421 gaagaagcag ccgaagttcc gcgtgcgcgg ctacacgcgg tgccagcggt gcggccgctc
      481 gcgggcggtc taccgcaagt tcatgctctg ccggatctgc ttccgtgagc tcgcgcacct
      541 cggggaactg ccgggggtga cgaaagcttc atggtgatga cgaaagcgaa gttctcctcg
      601 aacgtgagcg atccggtggc ggacctcctg acgaggatcc gaaacgcgaa cctggcgttc
      661 aaggacgaga cggtggcacc ggtgtcgaag ctcgcggact cggtcctcaa gatcctggac
      721 gccgagggct acatcatcgg gtactcgcgc gagggcgagg gcgtgcatca agctttccgg
      781 atccggctga agtacggcgt gcgtcggcgg cgcgcgatca ccggactccg gcggatctcg
      841 aagccgggac ggcggatcta ccgcggccgt gacgagctcc cgcgggtcca gggagggctc
      901 gggatcgcga tcatctcgac ctcgcatggg gtgatgacgg acaaggacgc cgctcgcaag
      961 ggtgtcggcg gcgaggtctt ggcctacgtc tggtaggtgg ccgagcgcgg acacaggcag
     1021 ggatgaggac gacatgagca ggatcggcag acaaccggtg gagatccccg agggggtcga
     1081 ggtgcgggtc gacgaggcca accaggtgac cgttcgtggt ccccggggtg agctctcgca
     1141 gaccatgcac cccacgatgc gcatcgtcac ggacgggggt gtcgcgcgcg tggagcggcc
     1201 cgacgacgtc gggttcagcc gcagcctgca cgggctcacg aggtccctcc tcgcgaacat
     1261 ggtcgaaggg gtcacgaagg ggttcgagaa gcggctgcag atcgtggggg tcggctatcg
     1321 cgccgccctg aaggggaagg acgtcgagct gcaggtcggc tactcccacc cggtgctcgt
     1381 gccgcagccg gacgggatcg agttcgaggt gccggctccc acgcaggtcg tggtgcgagg
     1441 gaacgacaag cagcaggtcg gagaagtggc cgccacgatc cgcaagatcc ggaagcccga
     1501 gccgtacaag ggcaaaggca tccgctacga gaacgagtac gtccggaaga aagcgggcaa
     1561 ggcagcgaag ggggcgacgg cctgatggac gccaagacga ggcgtgtcgc acgtgtccgc
     1621 cgtcactcgc gcgtccgcaa gaaggtcgcg gggaccgcgg agcggccgcg tctggccgtg
     1681 taccgctcga accggcacat ctacgtccag ctgatcgacg atcgcgcggc tgcgacgctg
     1741 gcggccgcgt ccgacaccga ggtcgccgtt tcgaagggca agaccgaccg cgccagagag
     1801 gtcggaaagc tcatcgccga ccgcgcgaag ggcgccggga tcgaccgggt cgtgttcgac
     1861 cggggtgggc ggcggtacca cggtcgcgtg gccgcggtcg ccgaaggtgc tcgagaggga
     1921 ggactccaga tctgatgtcg cgacgctatg actcgaggga ccaggagaag agcccgtacg
     1981 aagagcgggt catcttcatc aaccgggtgg cgaaggtcgt caagggcggt cgccgcttct
     2041 cgttcgcggc actgatcgtc gtcggcgacg gcgccggccg cgtcggcgtc ggctacggca
     2101 aggcgaagga ggtcccggcc gccatcgcca agggggtcga gctggcacgg cgcaagatgt
     2161 tcgaggtccc gatgatgggc acgacgatcc cgcatgagat cgtgggacag gccggtgcgg
     2221 ggcgggtgct gctcaagccc gccgcgccgg ggaccggcgt gatcgccggc ggtccggttc
     2281 gcgcgctcgt cgaagcggcc ggcatcaagg acatcctgtc gaagtcgatg ggctcttcga
     2341 accagatcaa catcgtgaag gcggcgatgg ccgggctcaa ggcgctccgg aagccgcacg
     2401 aggtcgcgat gatgcgcgac cgcgaggatc accaggtagc gcccaagccg atgctcgagg
     2461 ccgtcgccgc cgcggaggcg cacgctcagg cagcccgggc cgaggcactg acggaggtct
     2521 acggtgacaa ggcttaaggt gacccagacg cgcagcgtga tccgccggcc caaggaccag
     2581 aaggacacgg tccggcggct gggactccac aggatccgcg acagcgtgat caaggaggat
     2641 cgccccgaca tccgggggat gctcgtcaag gtccgccacc tcgtgcgcgt ggaggagttg
     2701 gcgggagacg atccgaagga tcgcgtggag aaggggaaga cgtagatgaa gctgcatcac
     2761 ctccgccccg ccaagggcgc gaagaaggaa cggacgcggg tcgggcgcgg ccgcgcgggc
     2821 gcccgcggga agaccgccgg gcgagggacc aagggcacgg gagcccgcgg cacgatccga
     2881 gccgggttcg agggcgggca gatgccgctg atccggcgtg ttcccaagct caagggcttc
     2941 aagaacccga accgggtcga gtacgcgccg gtgaacgtcg aggtcctggg ccggtacttc
     3001 gacggcgacg tcacccccga cgcgctgtat cggcacggac tcgcgcacaa gggagcgaag
     3061 atcaaggtcc tcgcccgcgg cgagctcgac aagaagctga cggtgaaagc gcacgcgttc
     3121 tcgaaagcgg cgaaggagaa gatcgaggcg gccggaggga ccgccgaagt cctcagctga
     3181 gctacgctaa gtcaccgtgc tgaaagcgtt cctcaacgcg ttccgggtgc cggacctccg
     3241 cgcgaagatc ctcttcacgc tgttcgtcat cgcggtcttc cgtctgggct cctacgtgcc
     3301 ggtccccgtc gtggacatcg gcatcctgca gagtcaggcg gacgcgggcg ggttcctgaa
     3361 catcatcaac ctgttctcgg gcggtgcgct gacccgcctg tcgatcttct cgctgggcat
     3421 catgccctac atcaccagct cgatcatcat gcagctgatg acggtcgtga tccccaagct
     3481 ccagcagtgg caggagcagg gcgagaccgg caccaagaag atcaaccagt ggacgcggta
     3541 cgtgaccgtc gtgctcgcgc tgctgcagtc cacgggcctg gtgttcctgt tccacaatcc
     3601 ggacgcgtcc gggttcgccg acgtgttccc gcagggcacg ttcaccggcg ccaacgtggc
     3661 cttcatcgtg ctgatcatga ccgcggggac cgccttcatc atgtggctcg gtgagctgat
     3721 cacgcagcgg ggcgtcggca acggcatgtc gatcctgatc ttcacgagcg tgatctccac
     3781 gctgcccgcg gaggggaacg cgatcctgca acagggagga accggcaagt tcatcgcgat
     3841 cctgctgatc ggtctcggca tcatcgtggc cgtgatctac gtggatcagg gacaacgccg
     3901 tgtgccggtc caatacgcga agcgggtggt gggccggaag atgacctccg ggggcagcac
     3961 gtatctcccg ctgaaggtga accaggccgg cgtgatcccg atcatcttcg ccacgtcggt
     4021 cctctacttc cccgccctgc tgagcagcgt gtaccacgcc gcgtggttcc agaacttcgt
     4081 gaacaaccac gtccagaa
//
LOCUS       DASUYD010000130         2864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1392460, whole genome shotgun sequence.
ACCESSION   DASUYD010000130 DASUYD010000000
VERSION     DASUYD010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1392460"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VF195_07125"
     CDS             complement(<1..207)
                     /locus_tag="VF195_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017622386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6580625.1"
                     /translation="MTGAEGLLNAKQLEELAEAGEVDTVLCMFTDLQGRFMGKRVVPH
                     FLLEEILGEEGLHACLYLLAIDMEM"
     gene            complement(321..1946)
                     /locus_tag="VF195_07130"
     CDS             complement(321..1946)
                     /locus_tag="VF195_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6580626.1"
                     /translation="MSERKVGSVTYQEVGEEYFKNRGLRRHAGVWSLWALGVGAVISG
                     EFYGWNFGLGTGGFGGLAIAAVLMAIMYYGLVYSIAEMSPALPHTGGAYSFARSAMGP
                     WGGFLTGLAENAEYVITPAVVVGAMALLMQEIVGGLFDVTGDPFWNSLPFWALIFYVI
                     FVGINIVGIEATMRFTVAITVLSLAVLAFFLLAAIFSGKLDFDLWTNIAKDGAEIPRG
                     GGPMLPFGIEGIFKSLPFAIWFFLAIEEVPLAAEESMDPRRDVPRGSVWGMHTLLIAA
                     ILVLVFNSALPGGAFLYGASGFPLLDGLYAIFGQNSATELLGLLFMIGLVASFFTIIF
                     AYGRNTYSLSRAGYFPKFLSQTHGVRKTPHVALIAGAVVGYAVFFLVWALQQQELGAQ
                     IVAALLNMAVFAAVISYILQMVSFVLLRQKLPNIERPYRSKWGVPGAVIAGLLAATSL
                     VAIFLNDAYRPGVYGVAIYYVLGVLYFAIAGRNRLVLSPEEEFALTQGEHGIPQEEGY
                     STSRSEQEAILGGGAPSAPSPASTQPPSETPTS"
     gene            complement(2013..2309)
                     /locus_tag="VF195_07135"
     CDS             complement(2013..2309)
                     /locus_tag="VF195_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580627.1"
                     /translation="MSALLATVATGHPAIPTGGEFDFTPLLYAAIGILAFRKLRPGLL
                     LLAAPLFLFAGGLVDSISDVLGLSTVTTASALVLPALLLPLPRQLGRHHRPSAG"
     gene            complement(2341..>2864)
                     /locus_tag="VF195_07140"
     CDS             complement(2341..>2864)
                     /locus_tag="VF195_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6580628.1"
                     /translation="LYNNAGVMMSQDGSVTETDVSIWDTTLGINVKGVAFGCKYGVPA
                     MIASGGGSIINVASFVAWMGAATSQTAYTASKGAVLSMTREIAVEFARRGIRCNALCP
                     GPIDTPLLAELLADPARRQRRFVHIPMGRLGQAEELAKAALFLASDDSSFMTGASLVV
                     DGGITAAYVTPED"
BASE COUNT          536 a          985 c          929 g          414 t
ORIGIN      
        1 catctccatg tcgatcgcca gcaggtagag gcaggcgtgc aggccttcct ctcccaagat
       61 ctcctcgagc aggaagtgcg ggaccacgcg cttgcccatg aagcggccct gcaggtcggt
      121 gaacatgcac aggacggtgt ccacctcacc cgcctcggcg agctcctcga gctgcttcgc
      181 gttcagcaat ccctccgcgc ctgtcacgtg ttccgccttt cggttcgggg gtcgctatcg
      241 aagaaccgcc gcacgacgct gtcaaacgcg aacggcgggc ccgagcacgg gcccgccgtt
      301 cgtaccgact cgccggtccg ctagctggtt ggcgtctccg atggaggctg tgtcgacgct
      361 gggctgggag cgctcggcgc tccgccgccg aggatcgcct cctgttccga gcgcgacgtg
      421 ctgtatccct cctcctgagg gatgccgtgc tcgccctgcg tgagcgcgaa ctcctcctcg
      481 ggcgacagca ccagacggtt ccgcccggcg atcgcgaagt agaggacgcc gagcacgtag
      541 tagatcgcca cgccgtacac accgggacgg taggcatcgt tcaggaagat cgcgaccagc
      601 gaggtcgcgg ccagcagccc ggcgatgact gcgccgggga ccccccactt gcttcggtac
      661 ggccgttcga tgttcggcag cttctgccga agcaggacga acgacaccat ctgcaggatg
      721 tacgagatga ccgccgcgaa caccgccatg ttgagcagtg ccgccacgat ctgcgcgccc
      781 agttcctgct gctggagggc ccagacgagg aagaacacgg cgtacccgac gaccgctccc
      841 gcgatcagcg cgacgtgcgg ggtcttccgc acgccgtggg tctgcgacag gaacttcggg
      901 aagtaccccg cccgcgagag cgagtacgtg ttccggccgt acgcgaagat gatcgtgaag
      961 aagctggcca ccaaaccgat catgaacagc aacccaagaa gctcggtggc gctgttctgt
     1021 ccgaaaatcg cgtacaggcc gtccagcagc gggaatccgg acgccccgta caggaaggcc
     1081 ccgcccggca gcgccgagtt gaagaccagg acgaggatcg cggcgatcag cagcgtgtgc
     1141 atgccccaca cggatcccct ggggacgtcg cgacgcgggt ccatggactc ctcggccgcg
     1201 agcggcacct cctcgatcgc caggaagaac cagatggcga acggcaacga cttgaagatc
     1261 ccttcgatcc cgaacgggag catgggccct ccgccgcgcg ggatctcggc gccgtccttc
     1321 gcgatgttcg tccacaggtc gaagtcgagc ttgccggaaa agatcgcggc gaggaggaag
     1381 aacgcgagca cggcgagcga gagcaccgtg atcgcaactg tgaaccgcat cgtcgcctcg
     1441 atcccgacga tattgatccc cacgaagatg acgtagaaga tcagcgccca gaacgggaga
     1501 ctgttccaga acggatcgcc ggtgacatcg aacagcccgc ccacgatctc ctgcatcagc
     1561 agggccatcg cgccgacgac caccgccggc gtgatcacgt actccgcgtt ctcggccagg
     1621 ccggtgagga atcctcccca cggccccatc gccgaccgcg cgaaggagta cgcgccgccc
     1681 gtgtgcggga gcgcgggcga catctccgcg atcgaataca cgaggccgta gtacatgatc
     1741 gccatcagga ccgcggcgat ggccaagccg ccgaacccgc ccgtgcccag gccgaagttc
     1801 cagccgtaga actcgcccga gatgacggct ccgaccccga gcgcccacag cgaccaaacc
     1861 cccgcgtgtc gccgaagccc gcggttcttg aagtactcct cacctacctc ttgataggtg
     1921 accgaaccga ccttccgttc ggacatacgc ccccctcccg agaacggcgg atgaagattc
     1981 agccgtatgg agccacaccc gaactttccg tgtcaaccgg ccgaagggcg gtgatgccga
     2041 cccagctggc gaggcagggg gagcaggagc gcggggagca ccagtgccga agccgtggtg
     2101 accgtggaca gcccgagcac gtccgagatc gagtccacca ggccccctgc gaaaaggaac
     2161 agcggggcgg ccagaagcag caggcccggg cggagcttcc tgaacgccag gatcccgatg
     2221 gcggcgtaga gcagcggcgt gaagtcgaat tcaccccccg tcgggatcgc ggggtgcccg
     2281 gtcgcgacgg tcgccaggag ggcgctcatg gcttcctatc gttcagtctg ggccacgagt
     2341 ttagtcctcc ggcgtgacgt aggcggccgt gatcccgccg tccacaacga gtgaggcccc
     2401 cgtcatgaac gacgagtcgt cgctcgcgag gaacagtgcc gccttcgcga gctcctccgc
     2461 ctgccccaac cgccccatcg ggatgtggac gaagcgccgc tggcgccgtg cgggatcggc
     2521 caggagctcg gcgaggagcg gggtgtcgat cggcccgggg cacagggcgt tgcagcggat
     2581 cccccggcgc gcgaactcga ccgcgatctc gcgcgtcatc gagagcacgg cgcccttgga
     2641 cgcggtgtag gcggtctggg acgtggcggc gcccatccag gcgacgaacg aggccacgtt
     2701 gatgatcgac ccgccacccg aggcgatcat cgccgggacg ccgtacttgc acccgaacgc
     2761 cacacccttg acgttgatgc cgagcgtcgt gtcccagatc gagacgtcgg tctcggtcac
     2821 cgagccgtcc tgactcatca tgaccccggc gttgttgtac agga
//
LOCUS       DASUYD010000131        18008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_511617, whole genome shotgun sequence.
ACCESSION   DASUYD010000131 DASUYD010000000
VERSION     DASUYD010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18008
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511617"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF195_07145"
     CDS             <1..328
                     /locus_tag="VF195_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580629.1"
                     /translation="GEIPRAIVGTIPFYGICALLGVGVGVLVRQPTIAIGVLVPWALF
                     AEQALGAFLPNLVTYLPFTAGEQLYAVTTRGEPLDAGVGAALFLTWTAVVVVAGSLLF
                     QRRDVA"
     gene            complement(302..946)
                     /locus_tag="VF195_07150"
     CDS             complement(302..946)
                     /locus_tag="VF195_07150"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6580630.1"
                     /translation="MPRRDTVVTAALAAATAALFALVATDPGRDLIQPIDDAFLRWVR
                     SVRTGWLTLIAKLFNLIGWTYVMTPVRIGVAAFLAIRRRRWHFIAFVSAIVVSEISIG
                     TLKALYDRPRALGSLVETSGASFPSGHAVAASVTVVAAVIALFPEGSRRYRWGAAAVA
                     FSSVLGLSRAYLGAHWLSDAVAGVLLGTSIALGTALIFHRVRERRASGDVASLE"
     gene            complement(1006..3210)
                     /locus_tag="VF195_07155"
     CDS             complement(1006..3210)
                     /locus_tag="VF195_07155"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX6580631.1"
                     /translation="MTNDPGVVSPGAAEASELVAVPDHEDTIRRTLKQRRPTGAPPPL
                     RRGMGASGRFWLGMMAYASIATVLVLQIDAVLHFSDRVETWWLLRVANLRVDWLTPIM
                     RGIKAAGSDWGTTVLGLGMVCALMIFRRWRHLLVFLGSLFAMQIVGGIVYELMSRPRP
                     YGVTIIGGWGGFALPSAPVAALTAVSIGIAYSLIVPGRPRWFAKLAIAFFIGLFVVAR
                     MYLAVDGPPGVVYGLILGVAFPLTAFRWFTPNEVFPVVYRKGSTAHLDVTGPRGEAIR
                     EAVQEQLGLTVLEIKPVGLEGSGGSTPLRLKVAGDPDTYLFAKLYAKSHVRADRWYKL
                     SRIVLYGSLEDEASFQSVRRFVEYEDYTLRLMTDEGISVPTPQGIVEISPEREYMIVM
                     EFFEGAREIGEVDVDEGIVDEGLSLIRKLWDAGLAHRDIKPANLMVRDGEVLLIDVFF
                     AQVKPSPWRQAVDLGNMMLVLAVRSDVDLVYRRALTFFTADEIAEAFAATRGIASPTQ
                     LRAFMKRDPRDLLGQFRALAPERRPISIQRWSVRRVLLALAMIGAFAIAVSASINAFL
                     PAQNLGIVRAPECGVSRASILSAQSVPTAAAIPCLDALPSGWIYGGGDIHSGEASFWL
                     NSDLAGMRAVTVTLRSACDVSSAHEVPSDEAGTRRFEAPSSLAPQLLNVRSYVFPGGC
                     ATYRFAFAAGAPTALVFDVDEAVSFVPRSVLVAHLREAEGLALCGRGAPCSP"
     gene            complement(3207..4055)
                     /locus_tag="VF195_07160"
     CDS             complement(3207..4055)
                     /locus_tag="VF195_07160"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580632.1"
                     /translation="MTPPRKPGAIRVAAFGFTESEIVAEVYSQALESKGYPVDPVLKA
                     GPRELVQPALAEGLIDLVPEYSGSALAFLTLGALEGTASTSETHDALTKVVTPSGVIA
                     LAPAPAQDANAIVVTQATADRFGLRDVSDLASVDSGLVFGGPPECAQRRLCLGGLEGV
                     YGLVFREFIPLDTGGPLTLQALRSGGIDVALLFTTDPAIVSERLVVLTDDRGLQPSEN
                     ITPLLRREVLSTYGEGVVPTLDAVSALLTTSDLRAMIGRVVGGSTAREVAASWLREHR
                     SALEGS"
     gene            complement(4133..5248)
                     /locus_tag="VF195_07165"
     CDS             complement(4133..5248)
                     /locus_tag="VF195_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HEX6580633.1"
                     /translation="MMPSVDRARRKRVIRVVQASLSALVLVAIFGLILPKIASYADVW
                     ETIKALTWIETVSLLAATAFNLFTYWWQMQSAMPGLTLGQAAVSNQTSTTVSNLLPGA
                     GVVATGLMYGMFRSWGFTTAEVALLASTTGIWNMFLKLGLPIVAVALLAITRQATDAL
                     LVPALIGLLVLAGSVVLFAMVLWKKSLARAIGARLGRIWSSIRGIFHKPPVETWGEAA
                     VRFRKQTIGLVVGRWPALTITTVASHLALWFVLLLSLRHVGVSEHEVSTVQALAVFAF
                     VRLISAIPITPGGVGVVELGLIGGLYAAGRTHADVPLDEFKVQVTAAALLFRTLTYGV
                     QIPLGGFTYLIWQRKKGWRKPEPEPPQPSAAPVRFPT"
     gene            5351..6244
                     /gene="ppk2"
                     /locus_tag="VF195_07170"
     CDS             5351..6244
                     /gene="ppk2"
                     /locus_tag="VF195_07170"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEX6580634.1"
                     /translation="MGDSKVKGTQRGHESPDRSTQDLIASPPKVPKKVYAAELLRLQE
                     ELVKMAEWVKASGARVVVIFEGRDAAGKGGAIKRITEHLNPRIARVVALPVPSERQTT
                     QWYFQRYVEQLPAGGEIVLFDRSWYNRAGIEKVLGFCTPEEHRMFLQQCPIFERLLVA
                     DGIHLTKYWFSVSDQEQERRFGARIDDPMRRWKLSETDLDSRTHWVDYSRAKDEMFVH
                     TDIPEAPWFVVPADDKRRARLNCMAHLLSRLPYVPKTLRELELPERQDDEGYVRPPRE
                     IYTYVPDHASIVVRAGPSQSD"
     gene            complement(6241..8091)
                     /locus_tag="VF195_07175"
     CDS             complement(6241..8091)
                     /locus_tag="VF195_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580635.1"
                     /translation="MLGRLSAVLLPIAIVGGLFLWPIASGDRRYPTGYDTTKYIFRAN
                     AVVADGVDSLDHITPPSLRLGTNPERPGYPVIASLTGGVLHMDSLGFAMVTPAVMAVA
                     IALSAGAFAVVLLRQPGWTFPLFAIATGASVMVAVTAVGGADNLIIDSFVLGLFVCSL
                     AFADRQAGFTGALLLSAGAALVHWSFGVLALALLSFVAVVFVGPSIRHARRGGAWWET
                     PALRLAAVIGGSVVVGTASMFVSPALPGKIPHNTPEKIQRKNAVRLPPLRLPAWAVAS
                     TAGAAASLFASDQRRRMTTVLLIGWGATPIAAALLFRLGSNLPPYRFAAFALALPILA
                     TLLVTDLGRLVARRMPVLGILVGAVLAVAPVALATSWSVDYWWGQPSIPEEQFDQSRA
                     AGRYLASLGEERPVVYITSRSEITLPDHVIRGGVPPSFVDDVYLFPGTLKELRFLQED
                     PEGELLSPTRNRRSLAARELIADVLVQEPIVLYLSAFNGQRPAPASLPEVQPGVFVVQ
                     GPSSVVEPAASPRTSDVVVDAFALAALLAAVGIGWSVALAPGDLLARAGLAMAFGLAV
                     LAVTGTIASRLGLPLRGASGTALVIATALGGWVVTASRHLRTRRAQHRKA"
     gene            8430..9182
                     /locus_tag="VF195_07180"
     CDS             8430..9182
                     /locus_tag="VF195_07180"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX6580636.1"
                     /translation="MTERKRAARRVVKKLFAAAGLDVSRRREHRMHLSMAEALRHLAG
                     SGFRPATVVDVGVGNGTSDLYASFPDATFLLVEPLEEFRPVLEDVADRYRALFVVAAA
                     GRERGSVDMFVHSYLEGSSLLRERGEQTGIVAREVPMVTIDDLCDEHDLAGPYLIKID
                     VQGGEIEAMSGATRTLADAEVVILEVSLFQFVEDGPELHDMIAYMKDRGFVAYDLFGG
                     HVRPLDGALAQVDIVFVKEDGRFRRSHAYGHA"
     gene            9179..10669
                     /locus_tag="VF195_07185"
     CDS             9179..10669
                     /locus_tag="VF195_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX6580637.1"
                     /translation="MSRCGRRRRWAAVGLVVAGLLGCSTPHDADPPVGRSPASVSVTP
                     SPPTTPSANYDWTTFGFDNERTGIDPSEVVLDVDAAPTLQVAWSADLGGAVTAPPVVV
                     GDVDADGESTSLAFVGTAAGDFFALDASDGHVVWRRDVGSVGSDCEEFPDGTHGVTGS
                     AVLDLEAGLVHVAGGTGSVFALDIATGAIAHGWPVPVTRSPELEHVFGALTLAGRLLY
                     VTMAGVCDIPPFKGKVTAIDVRTARRVATFYPTGEDGPSGGGIWGAGGASVDPATGDV
                     FVATGNAVADPEHFGFAEQVIRLTRHLKVRSANYPGITGVDSDFGSTPTLFQAPGCPP
                     QLAAVNKDGELFIYRREAISEGPAQRLQIADAEVQQLQGVPAYSPDTGLLYVTNPSDS
                     SDGAYRHGLLAFDVRPSCMIRLAWQKTLGRNNLIGSPPIVAGGVVYVAMAWDQRVYGF
                     DSVTGQELWNSGEAIQGAVFAPPVVANGRLFQGSWDGHLYAFEPSG"
     gene            complement(10725..12107)
                     /locus_tag="VF195_07190"
     CDS             complement(10725..12107)
                     /locus_tag="VF195_07190"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580638.1"
                     /translation="MTTSMCQVLGIVAVSRGTGEDAPVERAGPTRARAAWIDDVAARL
                     SRLGSDPGDDEDLRQKKALLVMLAVVILPVSVVWGSLYLGFGTWAGIIAFVYFAVSLS
                     SLVVFARTRNFRLLLVIQLLDILLTTTSGQMVVGGFLASGGVGLWGILAPLGALVFLE
                     VRRAIGWFVAFVLVFLLTGVAGEVLFPDADFPPWFSGAMLALNIIGAGAVAFTLLASF
                     AKQRNEALAALRVEQERSELLLMNILPSSIAERLKAASQTIADHFDAASILFADVVDF
                     TPFAQRLAPAEVVGILDQLFSHFDALVERHGLEKIKTIGDAYMAAAGVPDPSPDHAHM
                     AARLGLDMREAIATSAVGAGSGLELRIGINSGPVVAGVIGSKRFLYDLWGDAVNTASR
                     MESQSTPGEIQITRATYELLKDEFVCRSRGTIEVKGKGQMETWYLVGPRSDEGRTDLS
                     ADSEPSRSPA"
     gene            12441..13406
                     /locus_tag="VF195_07195"
     CDS             12441..13406
                     /locus_tag="VF195_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX6580639.1"
                     /translation="MISDRRSTPKISVVLALGAIVFASLMPAVRAPATPHMEAAARAP
                     EGLQQQVVRLFDELDEVKQSIETVQAETAWVRERISVLSRQIDARQQLLNRRAAEAYM
                     GERAVGFDSVLGAGSFTDLQDSLEFLDAVSQRDHEVLVSLEERKEEVERQWVRLEALE
                     EQLRGKQERLEATAESLVERLRQQHARLRELPEVGASDGGSGDSSHPPPPPGPPVPSV
                     VPGRAAVVALIRDHFAPLGSRTEAVALCVAEAESDFDPLAENPSTGAAGVFQFLPSTW
                     VNLSELAGRGGASVFDARANVAVTAWTVAGYGWHPWSSIAEACRV"
     gene            complement(13420..13545)
                     /locus_tag="VF195_07200"
     CDS             complement(13420..13545)
                     /locus_tag="VF195_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580640.1"
                     /translation="MIRPKAAYLLGGVAGLLGTALLVPLLRLLPQRAAVAVDPSI"
     gene            13672..14199
                     /locus_tag="VF195_07205"
     CDS             13672..14199
                     /locus_tag="VF195_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6580641.1"
                     /translation="METWDAIRTRRNVRSFDDRPLVDEHLDRIVAAAALAPSSMNEQR
                     WAFVVCRDRERLTELARVGDYTDHVAGAAAAIAFLTPKADDVAERESIAFDLGQAVEN
                     AMLAAWELGIGSCHGSVYDEPRIRDLLAYPPEMTCDLVVSFGYPKDPASLAPPGSRGG
                     RRPVGELRHEERFGG"
     gene            complement(14206..14556)
                     /locus_tag="VF195_07210"
     CDS             complement(14206..14556)
                     /locus_tag="VF195_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014210840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580642.1"
                     /translation="MASYSVNKRAVARARELIDAHRYVLESTWGDVQPSAEDENAYLE
                     SHSWDDYAGWFLGLTEGATDETKTRYGFVYGDFRRLHRSGLIACQYRAAEWRHKDVEL
                     AAHNLLQHLDSTRA"
     gene            14671..14826
                     /locus_tag="VF195_07215"
     CDS             14671..14826
                     /locus_tag="VF195_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580643.1"
                     /translation="MRRLLTLVPIAALLTACEDADNPGNDNLLTGGSLVLIVIIVVVV
                     ILMRRRG"
     gene            complement(14855..15136)
                     /locus_tag="VF195_07220"
     CDS             complement(14855..15136)
                     /locus_tag="VF195_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580644.1"
                     /translation="MAGDGTVERCDEHPCCFRAPGILAAPGFEHLAGDWHLQWWPDDS
                     AWFLVPPRGNVREVRVLGEADPPGELAVEGALFDDIRAEIARGGLFPPC"
     gene            complement(15151..16128)
                     /locus_tag="VF195_07225"
     CDS             complement(15151..16128)
                     /locus_tag="VF195_07225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03619 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6580645.1"
                     /translation="MRSRTPRTRTAHVHRLQRVAVALRVGVQLPEVEREVRWAEYVSM
                     ARAAEEVGFDSIWVGDHYLYRDDGRPERGPWEAWTLLAGLATVTERVSLGPLVACLNF
                     HSPAVLAKAAATVDELSGGRLVVALGAGWNRTEFDAFGIPFDHRASRFEEGFEIVRGL
                     LDGERVTFEGRFHRTRDAVLLPRPARRPPMMVGSTGARVLAATLPSVDVWNTWYDWYG
                     NTAEGFGSKIREIDDACERAGRDPATLERSACVLVRLGETAERPDEAGVTPLHGSPDA
                     IGAGLREIAAAGADELILVIDPITEGSIRALGPVVGELQRGAIVARRRA"
     gene            16156..>18008
                     /locus_tag="VF195_07230"
     CDS             16156..>18008
                     /locus_tag="VF195_07230"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HEX6580646.1"
                     /translation="MVERESQQLDPVRVDQPPSPARLVLLSFLMLFVELGLIRWSGAY
                     VVYLSFFTNFVLLASFLGVGIGFLRSRARSDLFRLFPGLLAALVLFLAVFPVQGGRVA
                     GRLQFVGGFGWPALPKWVSLGIVFLLAFLLMAAIAQGVGRVFVRFPALEAYRWDILGS
                     ILGIAAFSVLSFLHASPFVWGVIAAAIAVAVGAGWRAMQFVPLAVAVAVLGVLSFAPD
                     TLWSPYYRVTTRAGVDDGTVSINVNGRPHQRIMPLELMEETQAHRFGPYRHAPANPLT
                     DVLIIGAGSGNDVTIALAQGAERVEAVEIDPLLYRLGRDRHPDHPYQDPRVTVHIEDG
                     RSFLHDTERRYDLVLYAIPDSLTVLAGQSSLRLESYLLTLEAMREVRDHLKPNGVISM
                     YHYYLPVVVDRYADALTGVFGEPPCLELAAGSGPRPRTVLTASMRRDDLVCDERWSRP
                     TVLPAPVTDDHPFAYLVGHGIPAFYLVTLLAILLGSIAAVRLSAGPFGQMRPYVDLFF
                     MGAAFLLLETSNIVRFALLFGTTWFVNALVFAGILMSVSLAIEVARRVRFRHPGRLYV
                     PLFGAVVLAWAVPPESLLGLDFVPRFVAAIVLGFTPVFIANLVFADRFREVA"
BASE COUNT         2983 a         6039 c         6143 g         2843 t
ORIGIN      
        1 tggagagatc ccacgggcca tcgtgggtac gatccccttc tacggtatct gcgcgctcct
       61 cggcgtcggt gtcggggtcc tcgtgcggca gccaaccatc gcgatcggcg tcctcgtccc
      121 ctgggcgctg ttcgcggaac aggcgctcgg tgcgttcctg cccaacctcg tgacctatct
      181 accgttcacc gccggcgagc agctctacgc ggtcacgacg aggggagaac ctctcgatgc
      241 gggcgtgggc gcagcgctct tcctgacctg gaccgccgtc gtggtcgtgg cagggtcact
      301 gctattccag cgacgcgacg tcgcctgagg ctcgccgctc gcgcacacgg tggaagatca
      361 gggcggtccc gagtgcgatc gatgttccca acaggacccc ggcgaccgcg tcggagagcc
      421 agtgtgcccc caggtaggcc cgcgagagcc ccagcaccga tgagaaggcg accgccgccg
      481 ctccccaccg atagcggcgt gacccctcgg ggaacaacgc gatcaccgct gcgaccacgg
      541 tgaccgaagc ggcgacggca tgtccggaag ggaacgaggc gccgctcgtc tcgacgagcg
      601 atccgagcgc tctcggccgg tcatagagcg ccttgagcgt cccgatcgag atctcggaga
      661 cgacgatcgc ggagacgaac gcgatgaagt gccaccgcct tctgcggatc gcgaggaacg
      721 cggcgacgcc gatccgcacc ggtgtcatga cgtaggtcca tccgatcagg ttgaagagct
      781 tggcgatcaa cgtcagccat cccgttcgga ccgacctcac ccagcgaagg aaggcatcgt
      841 cgatcggttg gatcagatcg cgcccagggt ccgtggccac gagggcgaag agcgctgccg
      901 tggccgcggc gagcgctgcc gtgaccaccg tgtcgcgccg cggcatcccg agaagtggat
      961 gttccggatc gccctcgagg tccatgggtt ccacaggccg gcggctcacg gcgaacacgg
     1021 cgcgccccgg ccgcacaggg ccagaccctc tgcctccctg aggtgcgcca ccagaacgga
     1081 tctggggacg aacgacactg cctcgtccac gtcgaacacc aaggccgtgg gagcgcccgc
     1141 cgcgaaggcg aaccgatacg tggcgcaccc tccagggaac acgtaggagc gaacgttgag
     1201 gagctgcggc gcgagactcg acggagcctc gaacctgcgc gtgccggctt catcggaagg
     1261 gacctcgtgg gcgctcgaca cgtcgcacgc ggacctgagc gtgaccgtga ccgctcgcat
     1321 cccggccagg tcggagttca gccagaagct cgcctcgccg ctgtggatgt cgccgcctcc
     1381 gtagatccac cccgagggaa gagcgtcgag acaggggatc gccgccgccg tcggcacgga
     1441 ctgtgccgag aggatggacg cacggctcac cccgcactcg ggcgccctca cgatccccag
     1501 gttctgtgcc gggaggaagg cgttgatgct ggcagagacg gcgatcgcga acgcaccgat
     1561 catcgcgagc gcgaggagga cccggcggac gctccatcgt tggatggaga tcggccggcg
     1621 ctcgggcgcg agtgcgcgga actggcccaa gaggtcacga gggtcgcgct tcatgaaggc
     1681 tcggagctgc gtgggactcg cgatcccgcg ggtggcagcg aacgcctcgg cgatctcgtc
     1741 ggccgtgaag aacgtgagtg cgcgccggta cacgaggtcc acgtccgagc ggaccgccag
     1801 gacgagcatc atgttgccga ggtccacggc ctgccgccag ggtgacggct tcacctgcgc
     1861 gaagaagacg tcgatcagga gcacctcccc gtccctgacc atcaggttcg cgggcttgat
     1921 atcgcgatgc gccagacccg cgtcccacag cttgcggatc agcgacagcc cctcatccac
     1981 gatcccttcg tcgacgtcaa cctcgccgat ctcgcgagca ccctcgaaga actccatgac
     2041 gatcatgtac tcgcgttcgg gcgagatctc gacgatcccc tgcggcgtcg gcacggagat
     2101 gccctcatcc gtcatcaacc gcagcgtgta gtcctcgtac tccacgaatc gccggacgga
     2161 ctggaatgaa gcctcgtcct cgagcgagcc gtacagcacg atccgcgaca gcttgtacca
     2221 gcgatccgcg cggacgtggc tcttcgcgta gagcttcgcg aacagatacg tgtcggggtc
     2281 gcccgcgacc ttcaggcgaa gcggcgtaga accgcccgat ccctccagcc cgaccggctt
     2341 gatctccagc accgtgagtc cgagctgctc ctgcaccgcc tcccggatcg cttcgccgcg
     2401 tggaccggtc acgtcgaggt gcgccgtgct gcccttccga tagacgacgg ggaagacctc
     2461 gttcggggtg aaccagcgga acgccgtcag cgggaacgcc acgccgagga taaggccgta
     2521 gaccacgccc ggcggtccgt cgaccgccag atacatcctc gcgaccacga agaggccgat
     2581 gaagaaggcg atcgcgagct tcgcgaacca ccgggggcgc ccgggaacga tcagcgagta
     2641 ggcgatgccg atcgacaccg ccgtgagcgc ggcgacgggc gcggacggca acgcgaaccc
     2701 accccatcca ccgatgatcg tcacgccata tggacggggt cgggacatca gctcgtacac
     2761 gatccctccg acgatctgca tggcgaagag actgcccaag aacaccagca ggtgtcgcca
     2821 tctgcggaag atcatcagcg cgcacaccat ccccaagccg aggacggtcg tcccccagtc
     2881 ggagccagcg gccttgatcc cgcgcatgat cggggtcagc cagtccacgc gaaggttcgc
     2941 gacgcgaagc aaccaccagg tttcgacacg gtcagagaag tggagcaccg cgtcgatctg
     3001 aagcaccagg acggttgcga ttgaggcgta cgccatcatc ccgagccaga atcgaccgct
     3061 cgcacccatc ccgcgccgca ggggcggggg agcgcccgtc ggacgtcgtt gtttcagagt
     3121 tcgcctgatc gtgtcctcgt gatcgggtac ggccacgagc tcggaggctt cagcggcgcc
     3181 aggcgagacg actccgggat cgttcgtcat gaaccctcca aggcggatcg gtgctcccgc
     3241 agccacgacg cagccacttc gcgcgccgtg ctccccccca ccactcgacc gatcatcgct
     3301 cgaaggtccg aggtcgtgag gagcgccgag acggcatcca aggttggaac gactccctcg
     3361 ccgtacgtgg acagcacctc tcgacgcagg aggggcgtga tgttctcgga gggttggagt
     3421 cctcggtcgt cggtcaagac gacgagtcgc tcggagacga tcgcaggatc cgtcgtgaag
     3481 aggagagcga cgtcgatacc gcccgagcgc agcgcttgaa gcgtcagggg cccgcccgtg
     3541 tccagtggga tgaactcccg gaagacgagc ccgtagacac cctcgaggcc tcccaggcag
     3601 aggcgccgct gcgcgcactc aggtgggccg ccgaagacga gcccggagtc gaccgaagcg
     3661 aggtcgctga cgtcgcgaag gccgaaccga tcggccgtcg cctgcgtcac gacgatcgcg
     3721 ttcgcatcct gggcgggggc cggcgccagc gcgatcacac ctgatggcgt gaccaccttc
     3781 gtcagcgcgt catgggtctc actggtgctc gcggttccct ccagggcacc gagtgtgagg
     3841 aaggcgaggg ccgagcccga gtactcggga acgagatcga tcaaaccctc tgcgagtgcc
     3901 ggttgcacga gctccctcgg accggccttc agcaccggat ccaccgggta ccccttggac
     3961 tccaaggcct gggagtagac ctcggcgacg atctcgctct cggtgaagcc gaacgcggcc
     4021 accctgatgg cgccgggctt gcgagggggc gtcatggacg tgctgctcgt gcaccccgcg
     4081 acgacaacga gtacgccgag agcgaggaga gcgccatgac gaaagagagc cctcatgtcg
     4141 ggaacctcac gggggccgct gacggttgtg gaggttcggg ttccggtttc cgccagccct
     4201 tcttgcgttg ccagatcagg taggtgaagc cgccgagcgg gatctggaca ccgtaggtca
     4261 aggtccggaa caggagcgcg gcggctgtca cctgaacctt gaactcgtcg agcgggacat
     4321 ccgcgtgggt cctgccggcg gcgtagagac caccgatcag cccaagctcc accacgccca
     4381 cgccaccagg cgtgatgggt atggccgata tcagccggac gaacgcgaag accgcgaggg
     4441 cctgcaccgt gctcacttcg tgctcggaaa cgcccacgtg acgaagcgag aggagcagca
     4501 cgaaccacag cgcgaggtgg ctggcgacgg tcgtgatcgt gagcgcgggc caccgcccga
     4561 cgaccaaccc gatcgtctgc ttgcggaacc ggacggcggc ttcaccccac gtctcgactg
     4621 ggggcttgtg gaagatccct cggatcgacg accagatcct tccgagccgg gctccgatcg
     4681 cccgggcgag cgacttcttc cacagcacca tggcgaacaa cacgacgcta ccggcgagga
     4741 ccaggagtcc gatcagcgcc ggcacgagga gggcatccgt ggcttgacga gtgatcgcaa
     4801 ggagcgcgac ggccacgatc ggaaggccga gcttcaggaa catgttccag atgccggtcg
     4861 tcgaggcgag gagggctacc tcggcggtcg tgaagcccca cgagcggaac atcccgtaca
     4921 taaggccggt cgcgacgacg ccggcacccg gtaggaggtt cgagacggtc gtcgaggtct
     4981 gattgctcac cgccgcctgt ccgagcgtga gccccggcat cgccgactgc atctgccacc
     5041 aataggtgaa gaggttgaag gcggtggccg cgaggagcga gaccgtctcg atccacgtca
     5101 gggccttgat ggtttcccat acgtctgcgt agctggcgat cttcggaagg atcagtccga
     5161 atatcgcgac caagaccagt gcggacaatg aagcctggac cacgcggatc actcgcttgc
     5221 gtcgcgctcg atcgaccgat ggcatcatcg acgagcttgc ccccatctgg tcgaggtcac
     5281 aacccgagtt cgccggcgcc gtgggtcgcc gcgggggcga tgctctacgg tgttctcagg
     5341 agggcgcgag atgggcgact cgaaggtcaa gggaacgcaa cgtggccacg agtcacccga
     5401 ccgttccacc caagacctca tcgcgtcgcc tcccaaggtg ccgaagaagg tctacgcggc
     5461 ggagctcctc cggctgcagg aagagctcgt gaagatggcc gagtgggtga aggcgtccgg
     5521 cgcgcgcgtg gtcgtgatct tcgagggacg agacgcagcg gggaagggcg gcgccatcaa
     5581 gcgcatcacc gagcatctga atcccaggat cgcgcgagta gtcgcgctcc cggtgccctc
     5641 ggaacgacag acgacccagt ggtacttcca gcggtacgtg gagcaactgc ctgccggagg
     5701 cgagatcgtc ttgttcgacc gcagttggta caaccgcgcc ggcatcgaga aggtgctggg
     5761 gttctgcaca cccgaggagc accggatgtt cctccaacaa tgtccgatct tcgagcgact
     5821 cctcgtcgcc gacggcatcc acttgacgaa gtactggttc tccgtgagcg accaagagca
     5881 ggaacggcgt ttcggggcgc ggatcgacga cccgatgcgt cgctggaagc tctccgagac
     5941 ggatctggat tcgcgcaccc attgggtcga ctactcgcga gcgaaggacg agatgttcgt
     6001 gcacacggac atcccggagg caccgtggtt cgtcgtccca gccgacgaca agcgtcgggc
     6061 tcgcctcaac tgcatggcgc acctgctgag ccgcttgccg tacgttccga agacgctcag
     6121 ggagttggag ttgcccgagc gtcaagacga cgagggatac gtgcggcctc cgcgcgagat
     6181 ctacacgtac gtgcccgacc acgcctcgat cgtcgtgcgg gcaggtcctt cgcagagcga
     6241 ctaagccttg cgatgctgag cgcgtctcgt gcggaggtgg cgagacgccg tcacgaccca
     6301 gccacccagt gcggtggcga tgacgagcgc cgtccctgag gctccgcgca gcggcagtcc
     6361 aagcctgctc gcgatcgtgc cggtgacggc gaggacggcc aacccgaacg ccatcgccag
     6421 tcctgcccgg gccaacagat cgccgggtgc cagcgcgacg gaccagccga tgcccaccgc
     6481 cgcgaggagg gcggcgagcg cgaacgcgtc gacgacgacg tcactcgttc gcggcgatgc
     6541 ggccggctcg acgacgctgg agggtccctg cacgacgaac acgccaggtt gcacttccgg
     6601 gagactcgcg ggggccggcc gctgtccgtt gaacgcgctg aggtacagga cgatcggttc
     6661 ctggacgagc acatcggcga tgagctcacg cgcggccagc gaacgacgat tgcgcgtcgg
     6721 gctcagcagc tcgccctcgg gatcctcctg gaggaagcgg agctccttca gcgtgccggg
     6781 gaacagatac acgtcatcga cgaacgaggg tggcacgccg ccacggatca cgtggtcggg
     6841 gagcgtgatc tccgagcgtg aggtgatgta gacgacgggg cgctcctcgc cgagggaggc
     6901 gagataccgc ccggcggcgc gggactggtc gaactgctcc tcggggattg atggttggcc
     6961 ccaccagtag tcgacgctcc acgaggtcgc caaggcgacc ggcgccaccg ccaacaccgc
     7021 gccgaccagg atgccgagga ccggcatcct gcgcgcgacc aacctcccca gatccgtgac
     7081 gagcagcgtc gccaggatcg ggagcgccaa cgcgaaggcc gcgaatcggt agggaggcag
     7141 gttcgagccc agtcggaaca ggagtgcagc cgcgatcggg gtggcacccc atccgatcag
     7201 cagcacggtc gtcatccgcc gccgctggtc ggaagcgaac agcgatgcgg ctgcaccggc
     7261 cgtcgacgcc acggcccagg ctgggagtcg tagcggcgga agacggacgg cgttcttccg
     7321 ctggatcttc tcgggcgtgt tatgggggat cttgccgggg agtgccggcg agacgaacat
     7381 gctggcggtc cctaccacga cgctcccgcc gatgaccgcg gcgagccgga gagcgggtgt
     7441 ctcccaccag gccccccctc tccgagcgtg ccggatggac gggcccacga acacgaccgc
     7501 cacgaagctc agcagcgcga gtgcgagcac cccgaagctc cagtgcacga gcgcggcgcc
     7561 cgcgctcagc aggagcgctc cagtgaatcc cgcctgtcga tccgcgaacg cgagcgagca
     7621 gacgaacaac cccaacacga acgagtcgat gatcaggttg tcggcgccgc cgaccgccgt
     7681 gaccgcaacc atcaccgacg ccccggtcgc gatcgcgaag agtgggaagg tccaccccgg
     7741 ttgccgaagc aggaccacgg cgaaggcgcc ggcgctgagg gcgatggcga ccgccatgac
     7801 cgccggagtc accatcgcga agccgaggga gtccatgtgg aggacacctc ccgtgaggga
     7861 agcgatcacc gggtagcccg gccgttcggg attcgtgccg aggcgcagcg agggtggagt
     7921 gatgtggtcc agggagtcga cgccgtcggc cacgacggcg ttcgctcgga agatgtactt
     7981 ggtggtgtcg tacccagtgg ggtagcgccg gtctccgctc gcgatcggcc agaggaagag
     8041 cccgccgacg atcgcgatcg gcaggagcac ggcgctgaga cgaccgagca tcacccacgg
     8101 ggccgcccca tgctgcgctc ggacgtgacg ccccctgccc ctgccggccc atcgggagag
     8161 cgaccgctcc tcatcagatc gttccgtacg cgccgccgac cctcctgacc ttgcacggcc
     8221 ggcttcgatg gttcgtcacc gacctccgtc ctcgggttcg aagtatgaac cctcggcccg
     8281 cttggaggag aagacgtcgg tcgccggatc tcggttccgg atgcgctcct tccaccgatc
     8341 gctccgtcgc gcgtctgggt cggggctctg actgtggcat cgacgaagag gagttgtgcc
     8401 cggtcaggcc agcacctacc ttgcctgaca tgaccgagag gaagcgcgcc gcccgcaggg
     8461 tcgtgaagaa gctgttcgcc gctgcagggt tggacgtgtc ccgtcgtcgt gagcatcgga
     8521 tgcacctgtc gatggcggag gcactccgcc acctcgctgg ctccgggttc cggcctgcca
     8581 ccgtggtgga tgtaggggtc gggaacggca catccgacct ctacgcctcg ttccccgacg
     8641 cgaccttcct gctggtcgaa cctcttgagg agttccgacc ggtccttgag gacgtcgcgg
     8701 atcgataccg cgccctcttc gtcgtggcgg cagcaggacg agagcggggg agcgttgaca
     8761 tgttcgtcca ttcctacctc gaggggtcgt cactcctgcg cgagcggggc gaacagaccg
     8821 ggatcgtcgc acgcgaagta ccgatggtca cgatcgacga tctgtgcgac gagcacgacc
     8881 tggccgggcc gtatctgatc aagatcgacg tccagggtgg cgagatcgaa gcgatgtccg
     8941 gggcaacgcg gaccctggcg gatgcggagg tcgtcatcct ggaggtctcg ctcttccagt
     9001 tcgtggagga cggtccggaa ctgcacgaca tgatcgccta catgaaggac agagggttcg
     9061 tcgcgtacga cctcttcggg ggccacgtac ggccactgga cggggcactc gctcaggtgg
     9121 acatcgtgtt cgtcaaggag gatggccgat tccgccggag tcatgcctac ggccacgcat
     9181 gagccggtgt ggacggaggc gccgatgggc ggcggtcggg ctggtcgtgg ccggcctcct
     9241 tggatgctcg accccgcacg acgcggatcc tcccgtcggt cgttccccgg catcggtctc
     9301 tgtcacccct tccccgccga caacaccctc cgccaactac gactggacca ccttcggttt
     9361 cgacaacgag cgcaccggga tcgatccgag cgaggtcgtg ctggatgtgg atgccgctcc
     9421 cacgctgcag gttgcatgga gcgctgatct cggtggcgcg gtgaccgccc cgccggtggt
     9481 cgtcggagac gtcgacgccg acggggagtc caccagcttg gccttcgtgg gaacggcggc
     9541 gggtgacttc ttcgccctgg acgcgagtga cggccacgtc gtatggcgaa gggacgtggg
     9601 gtcggtggga tcagactgcg aggagtttcc cgacggcacg catggtgtga cggggtcggc
     9661 ggtgctcgac ctcgaggccg gcctcgtcca cgtggccgga ggcacgggca gcgtgttcgc
     9721 tctcgatatc gcgacgggcg cgatcgcgca cggttggccg gtacctgtga cccgttcgcc
     9781 ggaactcgag cacgtcttcg gggcgctcac gctcgcggga cgactcctct acgtgaccat
     9841 ggctggtgtc tgcgatatcc cgcccttcaa gggcaaggtg accgccatcg atgtgaggac
     9901 cgcacgccga gtcgccacct tctacccgac gggagaggat ggaccgagtg gcggagggat
     9961 ctggggcgca ggtggcgcat ccgtggatcc cgcgaccggt gacgtcttcg tcgcgacggg
    10021 caatgccgtg gcggatccgg agcacttcgg gttcgccgag caagtgatac gactgactcg
    10081 acacctgaag gtgcgatcgg cgaactatcc cggcatcacc ggagtggact cggacttcgg
    10141 ttccacaccc accttgttcc aggcaccggg gtgtccgccg cagctcgccg ccgtgaacaa
    10201 ggatggagag ctcttcatct atcggcggga agcgatcagc gaagggcccg cgcagcgact
    10261 gcagatcgcc gacgctgagg ttcagcagtt gcagggagtt ccggcctact cgcccgacac
    10321 gggcctcctc tacgtcacga atccgtcgga ctcgagcgac ggagcctatc gtcacggact
    10381 cctcgcgttc gacgttcggc cgagctgcat gatccgactt gcctggcaga agacgctggg
    10441 gcggaacaac ctgatcggtt cgccgccgat cgtcgccgga ggtgtggtgt acgtcgccat
    10501 ggcgtgggac cagcgcgtct atggattcga ctccgtgacc ggccaggagc tgtggaacag
    10561 cggggaagcg atccagggcg cggtgttcgc tccacccgtg gtcgcgaacg gacgcctctt
    10621 ccaggggtcg tgggacggac atctctacgc gttcgaaccc tcagggtgac gctccacctt
    10681 cacctgtcga ccttcctggg ggccaacgtt ccgatcgcgt ctgctcaagc aggagaccgg
    10741 gacggctccg agtcggcgct gaggtccgtt cggccttcgt cggaccgcgg tccgacgagg
    10801 taccacgtct ccatctggcc cttccccttg acctcgatcg ttccccgtga cctgcagacg
    10861 aactcatcct tcagcagctc gtacgtcgcg cgcgtgatct ggatctcccc gggcgtgctc
    10921 tgggactcca tccggctcgc cgtgttgacg gcgtcgcccc agaggtcgta gaggaagcgc
    10981 ttcgagccga tcactccggc gacgaccggc ccggagttga tgccgatcct cagctccaga
    11041 ccggacccgg cgccgacggc cgaggtggcg atcgcctcgc gcatgtcgag ccccaggcgc
    11101 gcggccatgt gggcgtggtc ggggctcggg tccggcacac ccgcagcggc catataggca
    11161 tcgccgatcg tcttgatctt ctcgagcccg tgtcgctcga cgagcgcgtc gaagtgggag
    11221 aagagctgat cgaggatgcc gacgacctcg gccggcgcga gccgttgcgc gaagggcgtg
    11281 aagtccacga catcggcgaa gaggatggac gccgcgtcga agtgatcggc gatcgtctgg
    11341 ctggctgctt tcagccgctc ggcgatcgag ctcggcagga tgttcatgag gagcagctcg
    11401 gatctctcct gctcgacgcg gagcgccgcc agcgcctcgt tgcgttgctt cgcgaaggag
    11461 gcgagcaggg tgaaggcgac ggccccggcg ccgatgatgt tcagcgccag catcgctccg
    11521 ctgaaccagg gcgggaagtc cgcgtcgggg aagaggacct cgcccgctac ccccgtgagg
    11581 aggaagacca gcacgaaggc gacgaaccag ccgatcgcgc gtcgcacctc caagaagacg
    11641 agggcgccga gcggagccag gatcccccag agtccgaccc ctccggacgc gaggaacccg
    11701 cccacgacca tctgcccgga cgtcgtggtg aggaggatgt cgaggagctg gatgacgagg
    11761 agaagccgga agttgcgggt ccgtgcgaag accaccaagg agctgagcga gacggcgaag
    11821 tacacgaacg cgatgatgcc cgcccacgtg ccgaagccga ggtagaggct gccccacacg
    11881 acggacacgg gcagtatcac gacggcaagc atgacgagga gcgccttctt ctgacgaagg
    11941 tcctcgtcgt ccccgggatc cgagcccaac cgggagagcc gtgcggccac gtcgtcgatc
    12001 cacgcagcgc gggctcgggt gggtccggcg cgctccacgg gcgcatcttc gccggttccc
    12061 ctggagacgg caacgatccc gagcacctgg cacatcgacg tcgtcaccgt cgatcgacct
    12121 tcgcatccga tcgccatccg tgtggaacgc gtaagtcgga tgggctactt ggacgtctcg
    12181 gttccgttct gagatcgtcg ggaacgcggg cgacccgaag gagcacgctt gggcccatcg
    12241 tcgggctcgc ggcgatggct ctcgtagcca tcacttccct ggcagcgcag gccggactcg
    12301 acacctgtct cggggaggtc gcgacgatca atcgggaccg aaggggatga catcctttcc
    12361 cgcgatggcc gggggccgtg gctccctcgt ccggggaggt ggtgtttgca tggtcgccca
    12421 ttcggatagg gtgctactcg ttgatcagcg atcgcaggag taccccgaag atcagcgtcg
    12481 ttctcgccct cggggcgatc gtgttcgcgt ccctgatgcc cgcggtgcgg gcccccgcga
    12541 caccgcacat ggaggccgca gctcgcgcgc ccgagggtct ccaacagcag gtcgtccgcc
    12601 tcttcgacga gctcgacgag gtgaagcagt cgatcgagac cgtgcaggcg gagaccgcgt
    12661 gggtccgtga gcggatctcg gtgctctcac gacagatcga cgcgcgacag caactgttga
    12721 accgacgcgc cgccgaggcg tacatggggg aacgtgcggt cgggttcgac tcggtgctcg
    12781 gtgccggctc gttcacggac ctccaggact ccctcgagtt cctcgatgcc gtctctcagc
    12841 gggatcacga ggtgctcgtc tcgctggagg aacggaagga agaggttgag cggcagtggg
    12901 tccgcctcga ggcgctcgaa gagcagcttc gggggaagca ggagcggttg gaagcgaccg
    12961 cggagagcct cgtcgagaga ttgcgacaac agcatgcccg ccttcgagag ctgcccgagg
    13021 tgggtgcgtc ggatggcggt tccggcgact catcccaccc gccacctcca ccgggccccc
    13081 ccgttcccag cgtggtcccg gggagggcgg ccgtggtcgc attgatccgt gatcacttcg
    13141 cccccctcgg ttcgagaacc gaagcggtcg ccctgtgcgt cgccgaagcc gagtccgatt
    13201 tcgacccctt ggccgagaac ccgagcaccg gagcagcggg cgtgttccag ttcctccctt
    13261 ccacctgggt gaatctctcc gagcttgccg gccggggcgg cgcgtccgtg ttcgacgcca
    13321 gggcgaacgt cgccgtcact gcctggaccg tcgcggggta cgggtggcac ccgtggagct
    13381 cgatcgccga ggcctgccgg gtctgacgag gatcccggat cagatgctcg gatcgaccgc
    13441 gacggccgcg cgctgaggca gcagcctgag caacggcacc agcagcgccg tgcccaacag
    13501 cccggccacg ccgccgagga gataggcggc cttcggcctg atcacggcac gagccgcggc
    13561 cacccaggcg gcggagccgg tctccgcata gaggggagta gctcagcgcg gtgccggatg
    13621 ccacgcgtcc gcgtgcgcgt aggctcctgc ggacgacgag agaggagctc gatggagacc
    13681 tgggacgcga tcaggacacg ccgcaacgtc cgttccttcg atgaccgacc gctcgtcgac
    13741 gagcatctgg accggatcgt cgcggcggcg gccctcgcgc cgagctcgat gaacgagcag
    13801 cggtgggcgt tcgtcgtgtg ccgggatcgg gagcggctga ccgaactcgc gcgcgttggt
    13861 gactacacgg accacgtcgc cggcgcagcg gccgcgatcg cgttcctcac tcccaaggcg
    13921 gacgacgtcg ccgagcggga gagcatcgcc ttcgacctcg ggcaagcggt ggagaacgcg
    13981 atgctcgcgg cgtgggagct cgggatcggt tcctgccacg gctcggtgta tgacgaacct
    14041 cggatccgcg acctgctcgc ctacccgccg gagatgacgt gtgacctggt ggtgtcgttc
    14101 ggctacccga aggaccccgc atccctggcg ccgcccggct cgcgcggggg acgccgaccg
    14161 gtcggggagc tccggcacga ggagcggttc ggcgggtgac ggaactcagg ctcgcgtgct
    14221 gtcgagatgc tgcaggaggt tgtgcgccgc cagctccacg tccttgtgtc gccactcggc
    14281 cgctcggtac tgacaggcga tcaagccgct ccgatggaga cgcctgaagt ctccgtagac
    14341 gaacccgtac cgtgtcttgg tctcatccgt ggcgccttcc gtgagcccca ggaaccaccc
    14401 cgcgtagtcg tcccacgagt gcgactctag atacgcgttc tcgtcctcgg cgctcggttg
    14461 cacgtcgccc cacgtgctct cgaggacgta ccggtgggcg tcgatgagct cccttgcccg
    14521 cgccacggcg cgcttgttca cggagtacga ggccatgcct gcatcctccc gccgatcgat
    14581 cggagaggaa agtcgctcgt tcgcggttcg gggacttgcc acccgggtac cacaggcggg
    14641 tagcgaacgt tccgatcatg gaggtctggg atgagaaggc tcctcacact ggtaccgatc
    14701 gcggcactcc tcacggcatg cgaggacgcg gacaaccccg ggaacgacaa tctcctcact
    14761 gggggatcgc tcgtgctcat cgtgatcatc gtcgtggtgg tcatcctcat gcgccggaga
    14821 gggtaaccgt ccgaggatcg atccccaggc gggatcagca gggagggaac agtcctccac
    14881 gagcgatctc ggcgcggatg tcgtcgaaga gcgcgccttc cacggcgagc tcgccgggcg
    14941 ggtcggcttc cccgaggacc cggacctcgc ggacgttgcc tcgaggcggg accaggaacc
    15001 aggcgctgtc gtccggccac cactggaggt gccagtcgcc ggcgaggtgt tcgaagcctg
    15061 gagcggcgag gatgccgggc gctctgaagc agcatgggtg ctcgtcgcat cgttcgaccg
    15121 tgccgtcgcc cgccatcgcc gttcacccct tcacgcgcgt cggcgagcga cgatagcacc
    15181 gcgctggagc tcgcccacca ccggaccgag ggctcggatc gatccttcgg tgatcggatc
    15241 gatgaccagg atcagctcgt ctgccccggc cgcggcgatc tctcggaggc cggcgccgat
    15301 agcgtcggga gaaccgtgga gcggtgtgac gcccgcctcg tccggccgtt ccgcggtttc
    15361 tccgaggcgg acgaggacgc acgcgcttcg ttccagggtg gccgggtcgc gtccggcccg
    15421 ctcgcacgcg tcgtcgatct ctctgatctt cgagccgaac ccttccgccg tgttcccgta
    15481 ccagtcgtac caggtgttcc acacgtcgac ggacggcagg gtggcggcga gcacgcgcgc
    15541 gccggtggag ccgaccatca tcggcgggcg cctcgcaggg cgggggagca gcacggcgtc
    15601 gcgggtgcga tggaagcggc cttcgaacgt gacccgctcg ccgtcgagca gccctcgtac
    15661 gatctcgaag ccttcctcga agcgcgacgc gcgatggtcg aacgggatcc cgaacgcgtc
    15721 gaactccgta cggttccacc cggcgccgag ggccacgacg agacgcccgc cgctcagctc
    15781 gtccacggtc gccgccgcct tggccaagac cgctgggcta tggaagttga ggcacgcgac
    15841 caagggacct agggagaccc gctcggtgac ggtcgcgagg ccggccagga gcgtccacgc
    15901 ttcccacggc ccccgctccg gacggccgtc gtcccggtag aggtagtggt cgccgaccca
    15961 gatcgagtcg aagccgacct cctcggcggc gcgcgccatg gacacgtact cagcccaccg
    16021 aacctcgcgc tccacctccg ggagctgaac gccgacacgc agggccactg ccacgcgctg
    16081 gagtctatgg acgtgcgcgg tcctggttcg gggcgtccgg gacctcatcg actcgctgct
    16141 agcatccgcc cccggatggt cgagcgcgag tcccagcagc tcgaccccgt acgcgtcgat
    16201 caaccaccgt cccccgcccg cctcgtgctc ctcagcttcc tgatgctgtt cgtggagctc
    16261 gggctgatcc ggtggagcgg cgcctacgtc gtctacctgt ccttcttcac gaacttcgtg
    16321 ctgctcgcga gcttcctcgg cgtggggatc gggttcctgc gctcccgtgc acgcagcgat
    16381 ctcttccgtc tcttcccggg tctgctcgcg gcactcgtcc tgttcctggc ggtgttcccc
    16441 gtgcaaggcg gccgcgtggc gggccggctg cagttcgtcg gcgggttcgg gtggccggcc
    16501 ctcccgaagt gggtctccct cgggatcgtc ttcctgctcg ccttcctgct catggccgcg
    16561 atcgcccaag gcgtggggcg tgtcttcgtg cgtttcccgg cgctggaggc gtaccgctgg
    16621 gacatcctcg ggagcatcct ggggatcgcg gcgttctccg ttctctcctt cctccacgcc
    16681 tcaccgttcg tgtggggggt gatcgcggcg gcgatcgcgg tcgcggtcgg tgccggttgg
    16741 cgggcgatgc agttcgtgcc gctcgccgtc gctgtcgccg tcctcggggt gctctccttc
    16801 gcgccggaca cgttgtggtc gccgtactac cgggtgacca cccgggccgg cgtggacgac
    16861 gggaccgtgt cgatcaacgt gaacgggcgt ccccaccagc ggatcatgcc cctcgagctg
    16921 atggaggaga cgcaggccca tcggttcgga ccgtaccggc acgcgcccgc gaacccgctc
    16981 acggacgtgc tcatcatcgg cgccggaagc ggcaatgacg tcacgatcgc gctggcgcag
    17041 ggcgccgagc gcgtcgaagc ggtggagatc gatccgctcc tgtatcggct cggccgcgac
    17101 cgccatccgg atcatccgta ccaggacccg cgggtcaccg tgcacatcga ggacggccgg
    17161 tcattcctcc acgacaccga gcgccgctac gacctcgtcc tctacgcgat ccccgattcg
    17221 ctcacggttc tcgccggtca gtcatcgctc cggctcgaga gctacctgct caccctcgag
    17281 gcgatgcggg aggtgcggga tcatctgaaa ccgaacggcg tgatctcgat gtaccactac
    17341 tacctcccgg tggtggttga tcggtacgcc gacgccctga ccggggtctt cggggagccg
    17401 ccgtgcctcg agctggcggc cggctccgga ccgaggccgc ggaccgtcct caccgcgagc
    17461 atgcggcgcg acgatctggt ctgcgatgag cggtggtcgc ggccgaccgt gctcccggca
    17521 cccgtcaccg acgatcatcc gttcgcctac ctggtggggc atgggatccc cgcgttctac
    17581 ctcgtgacgc tgctcgcgat cctcctcggt tcgatcgcgg cggtgcgtct gtcggccggt
    17641 ccgttcgggc agatgcgacc gtacgtcgac ctcttcttca tgggtgcggc gttcctgctg
    17701 ctcgagacct cgaacatcgt gcggttcgcg ctgctgttcg ggacgacctg gttcgtgaac
    17761 gcgctcgtct tcgccgggat tttgatgtcg gtctccctgg ccatcgaggt cgcgcggcgg
    17821 gtccggttcc gccatcccgg tcgactctac gtgccgttgt tcggcgcggt cgtgctcgcg
    17881 tgggcggtgc cgccggagtc gctcctcggg ctcgacttcg tcccgaggtt cgtggccgcg
    17941 atcgtcctcg gcttcacgcc ggtcttcatc gcgaacctgg tgttcgcgga tcggttccgc
    18001 gaggtcgc
//
LOCUS       DASUYD010000132         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1173422, whole genome shotgun sequence.
ACCESSION   DASUYD010000132 DASUYD010000000
VERSION     DASUYD010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173422"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..177
                     /locus_tag="VF195_07235"
     CDS             <1..177
                     /locus_tag="VF195_07235"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX6580647.1"
                     /translation="INSWAYARAYESSAERADALGTFIDFYNRERPHGGLNGTRPIDR
                     VRQRCRWEVQLGGS"
     gene            257..658
                     /locus_tag="VF195_07240"
     CDS             257..658
                     /locus_tag="VF195_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580648.1"
                     /translation="MQSTRGGQRALPRHVLRARPSATGRARQHVRSELSPILSASKVT
                     DAELMTTELVSNAIRHAGLPEGDSIGLEFSVELETVRVAVVDAGSGFASAPPATEEGI
                     GGWGLVLIERLSDRWGVHSAHPHSVWFEIER"
     gene            888..1130
                     /locus_tag="VF195_07245"
     CDS             888..1130
                     /locus_tag="VF195_07245"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580649.1"
                     /translation="MANAAEHSGLLEGDLIDLGIYVESQTVRISVVDGGPGLDQDRVI
                     LDADTRGWGLVLIDRISDRWGIHRAHPHSVWFEIDR"
     gene            complement(1245..1649)
                     /locus_tag="VF195_07250"
     CDS             complement(1245..1649)
                     /locus_tag="VF195_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6580650.1"
                     /translation="MKARTRAWRFRRFATHYLDPILRPLAEWVPTFGVMTHRGRTSGR
                     VYRTPINVFRRGNAYLFFLTYGADVDWVKNVLAAGGCCLRTRGSDVALVDPELIEDPE
                     LRAAPPIPRFIERRIAGVDAYLRMRAKAPGCR"
     gene            1838..2206
                     /locus_tag="VF195_07255"
     CDS             1838..2206
                     /locus_tag="VF195_07255"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6580651.1"
                     /translation="MATANDIDAYLAEVPEAHGRILRQLRGQIQKAVPEATETISYKI
                     PTFKYRGKALLYFASHKGHCSMYPVTDAILDVAGEELGDRITGKGTIRFTAEDPLPAR
                     LVSKIAEARRREIDETADAT"
     gene            complement(2447..3133)
                     /locus_tag="VF195_07260"
     CDS             complement(2447..3133)
                     /locus_tag="VF195_07260"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX6580652.1"
                     /translation="MTKRIIGLAVALATTMALAGTASAQASDPREFTLNVVHGIPGVV
                     VDVCVGGEKAITDFEAGEVVTGVKLPEGTYELGVTLAGEPCSAAILEVTAELEGGRFR
                     NYTVVANLDDAGTPNLLLFRNPMRKTEAGEARLVVRHTADAPAVTVWADGSRLNRGRQ
                     FVWSESRRFNVPEGDYEVFVSLAGQSDPVIGPVDLSLMAGFTYQVYAWGNAEAGYSLA
                     VIPVEVGQEH"
     gene            3725..3898
                     /locus_tag="VF195_07265"
     CDS             3725..3898
                     /locus_tag="VF195_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580653.1"
                     /translation="MRPKEPPPANEERKTVDPQPPGVDPERKEQRPSKPDDGAEAESA
                     PDSPEADVASHSE"
     gene            complement(3943..>4195)
                     /locus_tag="VF195_07270"
     CDS             complement(3943..>4195)
                     /locus_tag="VF195_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580654.1"
                     /translation="KIWFEWGSARFDAPGEGIPPAWFARRGTDPIESIPAIGLTEEEF
                     RRRLGELFDESTVEAVERDFEPRIAKTNLGPVLTPRPSR"
BASE COUNT          733 a         1385 c         1377 g          700 t
ORIGIN      
        1 atcaactcct gggcctacgc gcgtgcctac gagagctcag ccgaacgagc cgacgccctc
       61 ggcacgttca tcgacttcta caatcgcgag cgtccacacg gcggcttgaa cggcaccaga
      121 ccgatcgacc gggtccgtca acgatgccgt tgggaagtac agctaggcgg ctcataacga
      181 gcgctgcgta tgggtaagtc tacgcgtttc gagaaatggt cggttgcgac gtttgtggca
      241 gaggctgggc gggcagatgc agagcacgag aggaggacag cgagccttgc cccgccacgt
      301 gcttcgggcg cgtccctcgg ccacgggccg agcgcggcag cacgtgcgct ccgagctctc
      361 accgatcctc tctgcctcga aggtcaccga tgccgagttg atgacgacag agctcgtctc
      421 gaacgcgatc cgacacgcgg gtcttccgga gggcgattcg atcgggctcg agttcagcgt
      481 ggagctcgag accgtacgtg tggccgtggt ggatgccggg agcgggttcg cctcggcccc
      541 gccggcaact gaggaaggca tcggaggctg ggggctcgtg ctgatcgagc gtctatccga
      601 tcgctgggga gtccacagcg cccatcccca cagcgtctgg ttcgagatcg agcggtagag
      661 acgagaaccc tttccgctgc tgaatcgaca tccactctgg gtcggttgcg gtgatacgcc
      721 gttggccgga tactgccgaa gagcgggggt ccggctcgcg tgacccaccg ttcggttccg
      781 tcgggactcg atgcgcccgc tctcgcccgt tggcaggtcc gggaggatct cgccaccgtg
      841 ctcccaccca cgaaggttgt cgccgccgag ctgatgacgc cgagctggtg gccaatgcgg
      901 ccgagcactc cggactcctc gagggggacc tgatcgacct cggtatctac gtcgagtccc
      961 agaccgttcg catctcggtg gtcgacggcg gaccaggtct ggatcaggat cgagtgatcc
     1021 tcgacgcgga tacgcgcggc tggggtctgg ttctcatcga ccggatctcg gaccgctggg
     1081 gtatccatcg agcccacccg cacagcgtct ggttcgagat cgaccggtag ccgtcaagga
     1141 ggcctcactc cttcgcctga acgcggcgcc ctcgcatcgc ccccggcagc gccgccactg
     1201 ttcaccatcc gggcccaaag gttcgcggtt cctgctggag cgggtcagcg acatcctggc
     1261 gctttggctc gcatccggag gtaggcgtcg accccggcga tgcggcgctc gatgaagcga
     1321 gggatgggcg gggcggcccg gagctccggg tcctcgatca gctccggatc cacgagcgcc
     1381 acgtcggatc cccgcgtgcg cagacaacac ccccccgcag cgaggacgtt cttcacccag
     1441 tcgacgtcgg cgccgtaggt gaggaagaac aggtacgcgt tcccccgtcg gaacacgttg
     1501 atcggggtcc gatacacgcg cccgctggtg cggcctcggt gggtcatgac cccgaacgtc
     1561 gggacccact cagccagcgg gcgaaggatc ggatcgaggt agtgcgttgc gaagcggcga
     1621 aagcgccacg cgcgggtgcg ggccttcatg agggatcgtg ctcccaaagg gtacgttcgg
     1681 tcggtccgtc gcggccccac gctatccctt catcgcgtgc ggcgccgggg aagtcacctt
     1741 caccctccgg atcaagtacg gacgtttgtc gtcggttcaa cggggtagct caccatcgaa
     1801 aggcttcatc cggtggccct ggggggaggt tcgacagatg gccacagcca acgacatcga
     1861 tgcctacctg gcagaggtcc ctgaagctca tgggcgaatc ctgcgacagc tccgcgggca
     1921 gatccagaag gcggtccctg aggcgacgga gacgatcagc tacaagatcc cgacgttcaa
     1981 gtaccgtggc aaggcgctgc tctacttcgc ctcccacaaa gggcattgca gcatgtatcc
     2041 ggtaacggac gccatcctcg acgtggcagg cgaggagcta ggggatcgca tcaccgggaa
     2101 aggcaccatc cggttcacag ccgaggatcc gctgccggct cgcctcgtct cgaagatcgc
     2161 cgaggctcgc cggagggaga tcgacgaaac tgccgatgca acatgacgag cgcagctgat
     2221 cgacctacgt atgaacggac gtgccagcgg caacgccgcg tcccggcagt gctgcgccga
     2281 gatcgggcgt tacctcaacg tcggccggga gcgagccatg taggtcgcgc cggatcatcc
     2341 gacttcccaa ccgacaagtg gacgcactgg catctcagca caacgggaag agcggagggg
     2401 acggcgaccg atctcaccgt cccctccgct gatgatcgag cgctggtcag tgctcctggc
     2461 caacctccac tgggatcacc gctaggctgt acccggcttc cgcgttcccc cacgcgtaga
     2521 cctggtaggt gaacccggcc atcagggaga gatctacggg gccgatcacg ggatcactct
     2581 gaccggcgag cgacacgaag acctcgtagt caccttccgg cacgttgaaa cgccgtgact
     2641 cgctccacac gaattgccgt cctcggttga gccgcgagcc gtctgcccac acggtgacag
     2701 cgggagcatc cgcggtgtgc cgcaccacca gccgagcctc gcccgcttct gtcttccgca
     2761 tgggattacg gaagagcagc aggttcgggg tgcctgcgtc atcgaggttc gccaccaccg
     2821 tgtagttccg gaaccgaccg ccctcgagct cggccgtgac ctccaggatc gcagcgctgc
     2881 agggctcgcc ggcgagcgtg acccccagct cgtatgtccc ttcgggcagc ttgacgccgg
     2941 taacgacctc accggcctcg aagtcggtga tcgccttctc gccgcccacg cagacatcga
     3001 cgaccactcc cgggatgccg tgcaccacgt tgagcgtgaa ctcacgcggg tcagacgctt
     3061 gcgcggaagc cgttcccgcc agggccatcg tcgtcgcgag tgcgacggcg aggccgatga
     3121 tgcgcttcgt catgatccct ccttgatcca tctagccgtg tgatccaact gtgcttcttg
     3181 tcgaacgggc gatcggaacc catgcgcccc aggcgatcgg accttgcgat cgcgacgcgc
     3241 atcgcatggc tccggccatc gaagccagct attcccctgc tcgagttgtc ttggagcact
     3301 acccgcgacg cgaagtcgct aaccgattcg agtcgacttt ctccgtgtga tcatcaaagg
     3361 ggaccagtcg ggtggaagcc ggcaacgatc caggggcgga ggaccccgcg ctggaggagg
     3421 ctgccgagtc ctcctacccc cctggttcgg cggagatagg gctgctggct tgaggtgggg
     3481 aagatgcggc gtcgcggctg ggatgtcccg gctcgctcat ggggaaaccg aaccttcacc
     3541 cgcctcctcc gccgtgatgc atgagcaggg tggagcgatg cgcactcgat gaggtactcc
     3601 gccgtgatgc tcggttgctc acgctgtcca tccgaccctg agatccgaag cgagaacgac
     3661 cggccggccg gcgtggacgc tgcccggcgc gggtaacgcg ttggcgacgg agggaggaga
     3721 cgacatgagg cccaaggaac cgccgcccgc gaacgaggaa cggaagacgg tcgacccgca
     3781 gccacccggc gtagatcccg agcgcaagga acagcggccg agcaagccgg atgacggcgc
     3841 cgaggctgag tcagctcccg actcgccgga ggcggacgtc gcttcgcact cggagtagcg
     3901 gcacaccgat cactcgctcc ccgatcccgt ctggcacttc cgctaccggc ttggccgtgg
     3961 tgtgagtacc ggcccgagat tcgtcttggc gatgcggggc tcgaagtctc gctcgacggc
     4021 ttcgaccgtc gactcgtcga agagttcccc gagccgccgg cggaattcct cctcggtcag
     4081 gccgatggcg gggatcgatt cgatggggtc ggtccctcgg cgggcgaacc atgctggtgg
     4141 tatgccttct ccaggggcgt caaacctcgc gctgccccat tcgaaccaga tcttc
//
LOCUS       DASUYD010000133         8142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1835793, whole genome shotgun sequence.
ACCESSION   DASUYD010000133 DASUYD010000000
VERSION     DASUYD010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8142
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1835793"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..3180)
                     /gene="ileS"
                     /locus_tag="VF195_07275"
     CDS             complement(10..3180)
                     /gene="ileS"
                     /locus_tag="VF195_07275"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006131300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX6580655.1"
                     /translation="MANDDGPRFRPVDPKVRFPELEERILGFWEEADVFRRSIAQRES
                     APEWVFYDGPPTANAKPHIGHAVTRTFKDVFPRYRTMTGHLVHRKAGWDCHGLPVEIE
                     VEKEIGTSGKRDIEEFGVAEFNERCRVSVRRYVNDWEQLTRRIGFWIDMDDAYWTMDP
                     AYIESVWWSLKRLHERGLLVEADKVTAYCPRCGTALSDAEVALGYETAEDPSVYVRFP
                     IVDATDPDLVGVSLLVWTTTPWTLPSNAGVAVDAAASYVVAERDGDRVIVAEPLADPA
                     LGEGWTVRSTVQGEGLVGARYQPPYPNVEGAHTVVSGSFVQMSDGTGIVHLAPAFGPE
                     DLEVGRVQGWAVFKPVGDDGRFTDLAPAFIRGLFVKDADPAIIEELEARGLLLRAGTI
                     SHTYPFCWRCSTPLLYYARTSWYVRTTAVKDRLLEVNDAVDWHPDHIKHGRFGNWLEN
                     NVDWALSRERYWGTPLPIWRCGREHVTVVGSLQELGVHAGRELTDLDPHRPQIDDIVV
                     TCPDCGEEARRVPEVIDAWYDSGAMPFAQSGYHPELGRGEDTFERRFPADFISEAIDQ
                     TRGWFYTLMAEGVLHFDSPAYRAVVCFGFIVDEQGRKMSKSLGNVVDPLEVMQRHGAD
                     ALRWVLLTSGSPWSSRRLGDTVLDEAVRQFLLTLWHVYSFFVLYANADGADPDAAPAP
                     PPASRPLLDRWIQSRLAGTVRDVREAMDAYDVTGAGRRIRVFVDELSNWYVRRSRRRF
                     WNPGGHGGEDSVAAFHTLHECLITTATLLAPFTPFVAEELWSNLAAERAGRPDSVHLA
                     DYPRPHEAAIDPGLDEAMALARQVVELGRRIRTETRTRTRQPLPSASVHLSTDPGSFG
                     ALLDIVADELNVKQVDLAGTADVFGRWRAKPDFQVLGPRLGARVRAAASALAEDDGSL
                     AARLAAGQQVEMSVDDGPSIVIGPGDVALSQDVRSGYGVAAEGGVTVALELDVTSELR
                     REGVARELVRMIQDARKEAGFEVTDRIDLAVETSGEPAEALAAHREEIAEETLASELR
                     TEAVDGFRQVSELDGVPVVVTLERAT"
     gene            3709..4095
                     /locus_tag="VF195_07280"
     CDS             3709..4095
                     /locus_tag="VF195_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX6580656.1"
                     /translation="MTQTKLTAAEVKDLRARLTEERGHLESQLASIVEGSFAATQSDM
                     AGDVGLDDESADAGTATFEREKDLSIENNVRDLLRKIDGALRRMDAKTYGLCERCGKP
                     IEKARIKALPYAELCIKDAQAAQSRR"
     gene            4100..4597
                     /gene="lspA"
                     /locus_tag="VF195_07285"
     CDS             4100..4597
                     /gene="lspA"
                     /locus_tag="VF195_07285"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX6580657.1"
                     /translation="MNAPRSRAAQALFVAAFLAYGLDRVTKILAESSLPGSPVELVPG
                     VLTLRFTTNSGGAFSIGQSAPWFFVGVSAVVAVVILATAFRHTSRLVGASLGLVLGGA
                     LGNLTDRAVRAPGMRGRVVDFIDFQIWPVFNLADTAIVVGAILLAWTGLRERREESEP
                     AQAET"
     gene            4594..5490
                     /locus_tag="VF195_07290"
     CDS             4594..5490
                     /locus_tag="VF195_07290"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX6580658.1"
                     /translation="MKRTVEAEEGRLDVVVVRMTGMPRGDVQRAIAAGTVRVDGEPRE
                     KSFRLRGGERLTIEIEPDAPLSAEGPSVPIRYEDDELAVVAKPAGLVTHPTAGRRGGT
                     LVNRLLGMGVPLAPAGGTLRPGIVHRLDAGTSGLIVVAKTDEAYAALRRLFHDHAVDR
                     AYLALVRGSVGNDAFAVDAPLGRRASKIVVDATEGRRAETRFEVRERFDGATLLEAVP
                     RTGRTHQIRVHLAAIGHPILGDRAYGGGGNDARGLGLDRPFLHAGHLSFAHPITGRMV
                     TLDEGLPEDLRSALHRLHERLA"
     gene            5567..>8142
                     /gene="dnaE"
                     /locus_tag="VF195_07295"
     CDS             5567..>8142
                     /gene="dnaE"
                     /locus_tag="VF195_07295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX6580659.1"
                     /translation="MWKTPSPEVTTAARDGFVHLHVHSEYSLLDGAARIEAPASNRAA
                     PTIFSEAQRLAMPAVAVTDHGVMFGALGFYETALAHGIKPILGVEAYVAPGSRFDREP
                     GENEEKYHHLTLLARNETGYRNLLQLVSAAHLEGFYHRPRMDKQLLAEHAEGVICLSG
                     CLSSELSKLLLAGQDQRARGAAADYRDIFGAEHYWIELQDHGLAEQARVTPRQVALAR
                     QLGIGLVATNDLHYTTAADAKPHDVLLCIQQQKLQSDPKRLRFDAEEFYLKSSEQMRA
                     LFAELPEACDNTLAIADSVDLELIYGDGAAPEDRYHLPRFEAPDGGTLEDYLRELTGV
                     GAAQRYGDPLPQEIRDRLDLELGVILHMGLAGYFLIVWDLIRFAREQGIRVGPGRGSA
                     AGSVVSYCLRITDVDPLRYGLIFERFLNAERIQMPDIDMDFDERRRDEVIRYASAKYG
                     ADHVAQIVTFQTIKGKQGIRDAARVLGFPPAVGDRLCKMYPSPVMGRDHPIEEALKLS
                     PELSVAYEREPEAKEIVDTARALEGLRREDSVHAAGVVIGDAPLVRYLPLKLSKDSRD
                     DSKRIVTQFDMHGVEKLGLLKMDFLGLRNLSVIEDTLRHLRARGTELDIDHVPLDDAE
                     TYAMLRRGETTGVFQMESGGMRSLIKLLEPDRFEDLMALVALYRPGPLNNGLHTEYAE
                     RKHGRKPVSYPHPDLETILRGTYGVMVYQEQVMQIAVAMAGFSMGEADTLRKAMGKKK
                     IEYLMPFKERFVKGAVDRGYAERSAADMFEMFVPFADYGFNASHACAYGLVAYQTAYL
                     MAHHPVAYMASILTSVKDDKDRKPYYLYACRAMGIEVLPPDVNDSTLDFAPATDDDQA
                     IR"
BASE COUNT         1263 a         2837 c         2820 g         1222 t
ORIGIN      
        1 gatccgcggt cacgtcgcgc gctcgagcgt caccacgacc gggacgccgt cgagttccga
       61 cacctgacgg aagccgtcga ccgcctcggt gcgcagctcg gacgcgagcg tctcctcggc
      121 gatctcctcg cgatgtgccg ccagtgcctc ggcgggctcg cccgacgtct ccacagccag
      181 gtcgatgcga tccgtcacct cgaatccggc ttccttccgc gcgtcctgga tcatgcgcac
      241 cagctcgcgc gccaccccct cgcgtcggag ctcgctcgtc acgtcgagct cgagggcgac
      301 cgtcacgcct ccctccgccg cgacgccgta tccggagcgc acatcctggc tgagggcgac
      361 gtcgccggga ccgatcacga tcgacggccc gtcgtcgacg gacatctcca cctgctggcc
      421 ggccgccagt cgcgccgcga gcgacccgtc gtcctcggcc aaggcggacg ccgccgcgcg
      481 cactcgggct ccgaggcgcg ggccgagcac ctggaagtcg ggcttcgcgc gccagcgacc
      541 gaacacgtct gccgtgcccg cgaggtcgac ctgtttcacg ttcagctcgt ccgccacgat
      601 gtcgaggagg gctccgaacg atcccggatc ggtcgacaga tgcacggacg cggacgggag
      661 cggctgccgc gtgcgcgtcc gggtctcggt ccggatccgc cgaccgagtt cgacgacctg
      721 gcgggccagg gccatcgctt cgtccagacc gggatcgatg gccgcctcgt ggggtcgcgg
      781 gtagtcggcg aggtggaccg agtccggccg tccggcgcgc tcggccgcga ggttggacca
      841 gagctcctcg gcgacgaacg gcgtgaacgg tgccagcagc gtcgcagtgg tgatgaggca
      901 ttcgtgcagg gtgtggaacg cggccacgct gtcctcccca ccgtgcccgc cggggttcca
      961 gaaccggcgg cgcgaccgcc gcacgtacca gttcgagagc tcgtcgacga agacccggat
     1021 ccgccgtccc gctcccgtga cgtcgtaggc gtccatcgcc tcccgtacgt cacggacggt
     1081 tcccgccagg cgcgactgga tccagcgatc gaggaggggt cgcgaggcgg ggggcggggc
     1141 gggtgcggca tccgggtccg cgccgtcggc gttcgcgtag agcacgaaga aggaatagac
     1201 gtgccagagc gtcaggagga actggcggac cgcctcatcc agcacggtgt cgccgaggcg
     1261 gcgggaggac catggggagc cgctggtcag caggacccac cggagcgcat cggccccgtg
     1321 tcgctgcatg acctccaagg gatccaccac gttgccgagg gacttcgaca tcttgcggcc
     1381 ctgctcgtcg acgatgaagc cgaagcagac gaccgcgcgg tacgccgggg agtcgaagtg
     1441 caggacgccc tcggccatca gcgtgtagaa ccagccgcgc gtctggtcga tggcctccga
     1501 gatgaaatcg gcggggaacc gacgctcgaa cgtgtcttca ccgcgcccga gctccgggtg
     1561 gtagccggat tgcgcgaagg gcatcgctcc cgagtcgtac caggcgtcga tcacctcggg
     1621 gacccgtctc gcctcctccc cgcagtccgg gcacgtgacc acgatgtcgt cgatctgggg
     1681 gcgatgggga tcgagatcgg tgagttcgcg ccccgcgtgt accccgagct cctggaggga
     1741 cccgaccacc gtcacgtgct cgcggccgca ccgccagatc ggaagaggcg tgccccagta
     1801 ccgttcccgt gacaaggccc agtcgacgtt gttctcgagc cagttgccga agcgcccgtg
     1861 cttgatgtgg tcggggtgcc agtcaacggc gtcgttcacc tcgagcaggc ggtccttcac
     1921 ggcggtcgtt cggacgtacc acgacgtgcg cgcgtaatag aggagcggcg tgctgcagcg
     1981 ccagcagaac ggataggtgt gcgagatcgt gccggcgcgg agcaacaggc cacgggcctc
     2041 gagctcttcg atgatcgccg gatcggcgtc cttgacgaac agtccgcgga tgaacgcggg
     2101 agcgaggtcc gtgaagcgac cgtcgtcgcc gaccggcttg aagaccgccc agccctgaac
     2161 gcgtccgacc tcgaggtcct cggggccgaa cgccggcgcc aggtgcacga tgcccgtccc
     2221 gtcgctcatc tgaacgaacg aaccggagac gaccgtgtga gcgccctcga cgttcggata
     2281 gggcggctgg tatcgggcgc cgaccaaccc ctcgccctgc accgtgctcc gtaccgtcca
     2341 tccctcgccg agcgccggat ccgcgagggg ttccgcgacg atcactcgat cgccgtctcg
     2401 ctcggccacc acgtagctcg ccgccgcgtc cacggcgacg ccggcgttcg acgggagcgt
     2461 ccacggtgtc gtcgtccaca cgaggagcga cacgccgacg aggtccggat ccgttgcatc
     2521 gacgatgggg aaccgcacgt agacgctcgg gtcctctgcc gtctcgtagc cgagggcgac
     2581 ctcggcgtcg gagagcgccg taccgcaccg tgggcagtac gcggtcacct tgtccgcctc
     2641 gacgaggaga ccgcgctcgt ggagccgctt cagcgaccac cacacggact cgatatacgc
     2701 gggatccatg gtccagtagg cgtcgtccat gtcgatccag aagccgatcc ggcgcgtcag
     2761 ctgctcccag tcgttgacgt agcgccggac cgacacgcgg cagcgctcgt tgaactcggc
     2821 gaccccgaac tcctcgatgt cacgcttgcc ggaggtcccg atctccttct ccacctcgat
     2881 ctcgacgggg agcccgtggc agtcccatcc cgccttccgg tggacgaggt ggcccgtcat
     2941 cgtccggtag cgggggaaga catccttgaa cgtgcgcgtg accgcgtgcc cgatgtgcgg
     3001 cttcgcgttg gccgtcgggg gaccatcgta gaagacccac tccggcgcgc tctcgcgttg
     3061 cgcgatcgat cgacggaaca cgtcggcctc ttcccagaac cccaggatcc gttcctcgag
     3121 ctccgggaac cgcaccttcg ggtcgaccgg gcggaaccgc ggcccgtcgt cgttcgccat
     3181 ccgttcgcct cccgacacgc gaaacgcctc gtcctcgtct cgcgaggacg aggcgcgagc
     3241 cccgcggtac cacctcgctt gccggtcgcc tcgcggcgac ggccgctcac ggccgcgatc
     3301 acggcctgcc gctataacgg gcggacccgc ctgtgcctcc cacggaccga aaccggaccg
     3361 gttcgggcag gggctcgggg aggatgtccg cgaggcgctc ggcgccgggc tcccaccatc
     3421 cccggctcgc tcgtgccctg ccgtccccgc gacgcgtttc ccgtcgtcgc cttccgagtg
     3481 catgctacac cgcgccgcga gcggccgatg tccttcgtga tttctcggcg tgatgcctcg
     3541 atcggatggg acgggggacg ccgtcacctt cagccggggt cgacgcgcga gaaggtgctc
     3601 gaggtcgccg atctgagcag agttgaagtg ctgcaacaac tccggagaca ctcgatccag
     3661 agggggcccg ggactgaccg atgcgtcgac gtgctaccct tcgcccccat gacgcagacc
     3721 aagctcaccg cggcggaggt caaggacctc cgggcccgcc tcacggagga gcgcgggcat
     3781 ctggagtcgc agctcgcctc gatcgtggag ggctcgttcg cggccaccca gtcggacatg
     3841 gcgggcgacg tcgggctcga cgacgagtcg gcggacgccg gtaccgccac gttcgaacgc
     3901 gagaaggacc tctcgatcga gaacaacgtg cgcgacctgc tgcggaagat cgacggggca
     3961 ctgagacgca tggacgcgaa gacctacggc ctctgtgagc ggtgcgggaa gccgatcgag
     4021 aaggcgcgga tcaaggccct gccgtacgcg gagctctgca tcaaggacgc gcaggccgcc
     4081 cagtcgcgcc gctgatcgcg tgaacgcgcc gcggagccgt gcggcgcagg cgctcttcgt
     4141 ggcggcgttc ctcgcgtacg gtctcgaccg ggtcacgaag atcctcgccg agtcctcgct
     4201 ccccggcagt ccggtggagc tcgtccccgg cgtcctgacg ttgcggttca cgaccaactc
     4261 cggcggggcg ttcagcatcg gccagagcgc tccgtggttc ttcgttgggg tgagcgccgt
     4321 ggtggccgtg gtcatcctcg ccacggcgtt ccgtcacacg agccggctcg tcggcgcgtc
     4381 gctcgggctc gtcctcggcg gagcgctcgg gaacctcacg gaccgggcgg tccgagcccc
     4441 cggcatgcgt ggacgcgtgg tggacttcat cgacttccag atctggccgg tcttcaacct
     4501 ggcggacacc gcgatcgtcg tgggtgcgat cctcctcgcg tggacggggc tgcgggagcg
     4561 gcgggaggag tccgaacccg cgcaggccga gacgtgaagc ggaccgtcga ggcggaggag
     4621 ggccggctcg acgtggtcgt ggtccggatg accgggatgc cccggggcga cgtccaacgg
     4681 gcgatcgccg cgggaacggt ccgggtcgac ggggagccga gagagaagtc cttccgcctc
     4741 cggggagggg agcgcctgac gatcgagatc gagcccgacg cgccgctctc ggccgaggga
     4801 ccgtccgtgc ccatccggta cgaggacgac gagctcgcgg tggtggcgaa gcccgcgggg
     4861 ctcgtgaccc acccgaccgc cggccggcgc ggcgggacgc tcgtgaaccg gttgctcggg
     4921 atgggcgtcc cgctcgcgcc ggccggcggc acgctccggc ccggcatcgt gcatcggttg
     4981 gatgcgggca cctcgggtct catcgtcgtg gccaagaccg acgaggcgta tgcggcgctc
     5041 cgccgcctgt tccacgatca cgccgtcgat cgcgcctacc tcgcgctcgt ccgggggagc
     5101 gtcgggaacg atgccttcgc ggtcgacgcg ccgctcggac ggcgggcgtc gaagatcgtg
     5161 gtggacgcga ccgaaggacg ccgggcggag acgcggttcg aggtgcggga gcgcttcgac
     5221 ggcgccaccc tcctggaggc cgtgccccgc accgggcgga cgcatcagat cagggtccac
     5281 ctcgcggcga tcggccatcc gatcctcggg gatcgcgcct acggcggcgg ggggaacgac
     5341 gcccgcggcc tgggactgga tcgcccgttc ctccacgctg gccatctgtc cttcgcgcac
     5401 ccgatcacgg gcaggatggt cacgctcgac gaaggcctcc cggaggacct ccggtcggcc
     5461 ctccaccgac tccacgagcg gctcgcttga cggcgccgga gggcgacgct agcgtgggcc
     5521 ccccacgaat cacaggcatg taagtttcca cagccaccgg ggccggttgt ggaaaacccc
     5581 ctctccggag gtcaccacgg cggcgcgaga cggcttcgtt catctgcacg ttcactccga
     5641 gtactcgctg ctggacgggg cggcgcggat cgaggcccct gcctccaacc gagccgcgcc
     5701 cacgatcttc tcggaggccc agcgcttggc gatgcccgcc gtcgccgtca ccgaccacgg
     5761 ggtcatgttc ggagcgctcg gcttctacga gaccgcgctg gcgcacggga tcaagccgat
     5821 ccttggcgtg gaggcctacg tggcgccggg ctcccggttc gaccgcgagc cgggcgagaa
     5881 cgaggagaag taccaccatc tgacgctgct cgcgcggaac gagaccggct accggaacct
     5941 gctgcagctc gtgtcggcgg cgcacctcga gggcttctac caccgccccc ggatggacaa
     6001 gcagctgctc gcggaacatg cggaaggggt gatctgtctc tccggttgcc tgtcctccga
     6061 gctgtcgaag ctgctgctcg ccggccagga tcagcgtgct cgaggggcgg ccgccgacta
     6121 tcgcgacatc ttcggggcgg agcactactg gatcgagctc caggaccacg gcctggccga
     6181 acaggcgcgg gtgacgccca ggcaggtcgc cctcgcgcgc cagttgggga tcgggctggt
     6241 cgcgacgaac gacctccact acacgacggc cgccgacgcg aagccgcacg acgtgctcct
     6301 gtgcatccag cagcagaagc tgcagtccga tccgaaacgg ctccggttcg acgcggagga
     6361 gttctatctg aagtcctcgg agcagatgcg ggcgctgttc gccgagcttc ccgaggcgtg
     6421 cgacaacacg ctcgcgatcg cggactcggt ggatctcgag ctgatctacg gcgacggcgc
     6481 cgctccggag gatcggtacc acctcccgcg gttcgaggcg ccggacggcg ggacgctgga
     6541 ggactacctc cgggagctga ccggcgtggg cgcagcccag cgctacggcg atccgctccc
     6601 gcaggagatc cgtgaccgcc tggacctgga gctcggcgtg atcctccaca tgggactcgc
     6661 gggctacttc ctgatcgtgt gggacctgat ccggttcgcg cgggagcagg ggatccgcgt
     6721 cggacccggg aggggctccg ccgccgggtc cgtcgtgtcc tattgcctgc gcatcaccga
     6781 cgtcgacccg ctccgctacg ggctgatctt cgagcggttc ctgaacgcgg aacggatcca
     6841 gatgccggat atcgacatgg acttcgacga gcgtcggcgc gacgaggtga tccgctacgc
     6901 gagcgcgaag tacggagccg accacgtcgc gcagatcgtc acgttccaga ccatcaaggg
     6961 gaaacaaggg atccgcgacg cagcccgcgt gctgggcttc cccccggccg tcggggaccg
     7021 gctctgcaag atgtatccga gccccgtgat gggtcgcgac cacccgatcg aggaggcgct
     7081 gaagctgtcg ccggagctgt ccgtcgccta cgagcgtgag ccggaggcga aggagatcgt
     7141 cgacactgcc cgcgcgctcg agggactgcg ccgcgaggac tcggtacacg ccgccggcgt
     7201 cgtgatcggc gatgcgcccc tcgtgcggta cctgccgctg aagctgtcca aggattcgcg
     7261 ggacgactcg aagcgcatcg tcacccagtt cgacatgcac ggcgtcgaga agctcggtct
     7321 gctcaagatg gacttccttg gactacgcaa cctgtcggtg atcgaagaca cccttcggca
     7381 cctccgggcg cggggaaccg agctcgacat cgaccacgtg cccctcgacg acgccgaaac
     7441 ctacgcgatg ttgcggcggg gcgagaccac cggggtgttc cagatggagt ctggcgggat
     7501 gcggagcctg atcaagctcc tggagccgga ccggttcgag gacctgatgg cgctcgtggc
     7561 cctgtaccgg ccgggccccc tgaacaacgg cctgcacacc gagtacgcgg agcgcaagca
     7621 cggccgcaag cccgtctcgt acccgcatcc cgacctcgag acgatccttc ggggcacgta
     7681 cggggtcatg gtctatcagg agcaggtgat gcagatcgcc gtggcgatgg ccggcttctc
     7741 gatgggcgag gccgacacgc tgcgcaaggc gatgggcaag aagaagatcg agtacctgat
     7801 gcccttcaag gagcggttcg tgaagggtgc cgttgaccgc ggctacgcgg agcgttcggc
     7861 cgcggacatg ttcgagatgt tcgtcccgtt cgcggactac ggcttcaacg cctcccacgc
     7921 gtgcgcgtac ggcctggtcg cgtaccagac cgcgtacctg atggcgcatc accccgtcgc
     7981 gtacatggcg tcgatcctca catccgtgaa ggacgacaag gatcgcaagc cctactacct
     8041 gtatgcatgc cgtgcgatgg gcatcgaggt gctccccccg gacgtgaacg actccacgct
     8101 cgacttcgcc cccgccaccg acgacgatca ggcgatccgc ta
//
LOCUS       DASUYD010000134        23801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1174883, whole genome shotgun sequence.
ACCESSION   DASUYD010000134 DASUYD010000000
VERSION     DASUYD010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23801
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174883"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VF195_07300"
     CDS             complement(<1..527)
                     /locus_tag="VF195_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013133509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6580660.1"
                     /translation="MEYTHVGRLGLVVSRLCLGTMNFGPHTPEDESHRIMDAAIAEGI
                     NFFDTANVYGRHLGVGSTERIIGDWFAKGGGRREKVVLATKVYGQMAEWPNESRLSKL
                     AIRRACEGSLRRLQTDYLDIYQMHHIDRNAWWNEIWEAMDQLKSEGKILYVGSSNFAG
                     WHIARGNEIASKWHTV"
     gene            complement(537..1841)
                     /locus_tag="VF195_07305"
     CDS             complement(537..1841)
                     /locus_tag="VF195_07305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580661.1"
                     /translation="MSDRPSSFRDRIEILKPLAVRDFAFLWTGMTVSMIGDGIYLVSI
                     AWEVLSIENRPGALALVGVAWSLPQVVLVLASGALSDRLERRHLMIAGDAIRLVAIGT
                     LGVLSITDRLDSIPIILALVTVYGAGQAVFQPAFSSITPAIVPADLLVQANSLAQLVR
                     PFAMTLVGPLVGGVLISVAGDRAGLAFLVDAATFAFSAVMILSMRTRRVPRNGQEEQA
                     SFLADVGEGLRYVKEQRWLLIAMVAATVSLLAVWGPWETLVPVVVKNDLGGKAWALGL
                     VFGAGGFGAVIAAAAFGQRGRLPRKPLTAMYLAWALGMFGTAGFGVVTGVWQAMGVAM
                     ITEASITYLVVVWVTLVQRLVPDRLLGRVFALDWMISTMGVPLSFAITGPTAELIGHD
                     ATLIWAGAIGGTITLVFMFVQGARDPERDGSLAAAERSVTPA"
     gene            complement(1891..2622)
                     /locus_tag="VF195_07310"
     CDS             complement(1891..2622)
                     /locus_tag="VF195_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6580662.1"
                     /translation="MLRARVDRPRCIGAGNCITIAPTAFDWHRGDFGKAGVVDAASVD
                     EEKLREAALACPTQAIVLEEVAELLPWQLRGRSPTQRVQRTFMFTDIVGSTSLLEAMG
                     DEAWQSLLSWHDKTLRALFGANRGEEVVATGDGFFVAFESPDDALACAVAIQRGLAEH
                     RNSAGFAPQVRIGLHASDATMVGRNFTGKGVHEASRIGGLAEGSQIVASAETAVGGQF
                     PTADPRTVTVKGISDPIEVVTVDWR"
     gene            complement(2622..5849)
                     /locus_tag="VF195_07315"
     CDS             complement(2622..5849)
                     /locus_tag="VF195_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580663.1"
                     /translation="MIEAPPVAAAAFAPLGLFEHRTERAVVGRPAELAAIEDAIGGAE
                     GGRLTALTVEGEPGIGKTRLLLAAREMAGARGFLTIAVGADEELRGPFLLARSIVGAP
                     EVMEAVSGSPSEQAIIAASSALGGRDDPAFAGMSADQRLLRSFDLATMAVRALTAAQP
                     LALLIDDMQWADEDSLRLLRYVIRTEGASPIFVMFSVRPEELATLTEAVTLVADMERM
                     RVIRRLKLARFSVVETSEFLRQILGGQVEPATASTIHGQAEGVPFIVEEIARAYREAG
                     MLQQAADAWTLGRNAERLAPSAVRTLIQRRAAHLPVESKTALAEAALMGRNFSLKDLR
                     EVRLRIGEGGPDGDLVSLGDLMAPAVAAGLLVEQPEDSPADYTFPHEQVREFAADTLA
                     PARRREIHAAIVQLLLTGEPAPETLPLLAHHARAAGDAQVCVRFSTEAAAAALAAHAP
                     DEVLRVVEIALPQAATPQERLGLLTARDDALAMLRRPADRLEGLSELAALAEALGDPA
                     VDLDVTLRRSAALRLSQQEDRAAELAARVRTLAVERGDRPSELAACIALGQALLHAGV
                     GEGFTPSAAEVDLDAAEEAYARAVVLAEELGDRQMLAAATRELGVIDLGRVRGWFVEQ
                     VLVGAHLPVMERVAAGESLDDLVEELPVAPWVRSSGERLHRALALFEELGDRQGTMAT
                     IIAMAYLSWAPDIHIGADSARHIEEIRRLATQLASFTSESQRAFAEVQMLYGVHVFAR
                     AKVIPDLALSRGAEAYERARAIGDRQLEFLAAGGTAMSNVELGDLDEAITWLDRAATV
                     ALESPTPARGRQLELWRGLTDAARGDAAGMREHLERAVQMAADQGLPAARCEALTALA
                     LAAASLGAEREDEELRSLAERSAASAKEFLPLLPGHAPWGAKADSALAAVALSRGEPD
                     RAVEAARSAVEALRAAQHEDLNLDVLVPVAAAITAAGADEERAGTVSYLRLQAAVIAN
                     RIVDEGIRSRWFRGPTGSALAGLALDPDAPIVRTAKDVGVDGLGSDDADLLKLVVQGL
                     SNEEIADRLDVDPGEITRRLASMYASIGASSRADATAFAFQSGIL"
     gene            6249..6683
                     /locus_tag="VF195_07320"
     CDS             6249..6683
                     /locus_tag="VF195_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580664.1"
                     /translation="MRTLRAVTAALLLALLHSGSAACTSSNGGPSVSASPSLDVAPGG
                     YVYRSEEIEATIEITGEAGTLTVFNGTGFPLDDPGVYLLDARDGSRVDAEVIDAAPVP
                     DGETGEFEVTIPGAPEAKHVGLIVLLAGDADLGAFVPAGDQA"
     gene            6680..8338
                     /locus_tag="VF195_07325"
     CDS             6680..8338
                     /locus_tag="VF195_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6580665.1"
                     /translation="MNRTGITIAAVLALTALVVQDGTALSQEDAQEQRLIDTVCAIPP
                     VLLERTLHGHREDRAGEIQLLTREPDFVGHGGLPHSGPWDYVGQVPLLWYGPGYVPAN
                     GPIDRRVTLADLAPTQAELLGFDFDAPDGKPLLDALVPREDRPEPPALLVVLVWDGAG
                     NNVLKAHPDAWPNLRELMDEGVWYRRAEVGSSPPSTAQIHATIGTGAFSATHGLIAHH
                     FRIGDRIVTPWSAGPRYLVRPTLADLYDRALGNRPLIGATATVAIHLGMLGHGSLWGG
                     GDRDLAVLREREGASTLGAEGIRWNLTSSVAPFYHLPGYVNDLPPLSTYTEDVDRADG
                     RLDGKWRDVVIESEAVHFGLLTPARIPFQDRLIEELIATEGFGDDDVSDLFYINKKLI
                     DEVGHIFSMNSVEMEDSIRAEDAALPGLIEMLNREVGEGRWVMALTADHGHTPDPAVS
                     GADVISPTDVGRQITEGFDRDGDDVSVLEYTQPTEVFINERELADEGHTLADVADYLM
                     GLTKGDVGGDIWPVPEEHRAEPAFLAAYPSELLDRLPCLPPGEG"
     gene            8351..9685
                     /locus_tag="VF195_07330"
     CDS             8351..9685
                     /locus_tag="VF195_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580666.1"
                     /translation="MTRRAAILLVVAAIVAAPGIVLQATCPGDSCARSGPARTPFCSL
                     PEELRTRIAAGYREGRSPDVLAVTGGTGVVGSVPAVGAPTTVGSWPSTSSPIDTRTPI
                     VFRGAGVRGASSVPAGTELRQIAPTIAQILRFDRPFPGVRSGRPIPGIAGPDVPRLVL
                     AIVWKGVGTADLENDPDAWPFLRGLVSSGTGTLDGEVGSLPMDPSAGLTTIGTGGLPS
                     EHGITGGLFRGDRAEVVAAWAAGAPTSVIATLPDDLDRAFAQRPLIGIVGSDPADRGI
                     VGSGWYAERDRDDRVTAVAGQARLRAVNRLLASGYGRDDVPDVLAVVMEGSVGSLDAE
                     LRRTVRAARSATAGQVLIVVTATGAGTAPGSMQIEAADVERIVEAEIATDEPLVQAVV
                     PAGFYLDQRAMTAAGVTGQIAQDAALAARGPDGSAIFVDAFQGFAVPFGRYC"
     gene            9685..10698
                     /locus_tag="VF195_07335"
     CDS             9685..10698
                     /locus_tag="VF195_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEX6580667.1"
                     /translation="MTELVATPSGRRPISPRLAGLMIVALVLLGASIRLFGPTRVDWL
                     RAALVVFGSLVIQAVPFVLVGATAAALIEVFVPIGAFERIARLPRPLRLPAAGLAGVL
                     LPICECGSVPVARRLIHRGLDPSSAFTFMLAAPVVNPVVIASTFYAFRGRDTLWTMVL
                     GRTGLGLLVAMAVGWALGALGKEQVLRPVDEEDRGHSLELGRPEPRWRRFFVHLADDF
                     LFMGGYLLLGASMAAIIQTFLPQGLLERIGGTPVVDIVLMMGIASLLSLCSESDAFIA
                     ASFAQFGASSQLAFLVFGPMVDLKLGALYAGSFRRVVVQTIVAVAAVTTLIGSIWIEV
                     LTG"
     gene            10695..11408
                     /locus_tag="VF195_07340"
     CDS             10695..11408
                     /locus_tag="VF195_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEX6580668.1"
                     /translation="MSGRSFSQRRLAAASVLAAWSGLFWFLIITDRVDLYLSTRTSWV
                     VPMAAGGLAAVALGLVLAGRSSRPEPVRVREAVIMTLLVTPVVLLVTLPPSTLGSFSA
                     PKKAQFSGSALSTIYGRFDETSEITMLTVAAGETSDEGSRLLATRAGAEVGFVGFVAR
                     DMSAEADELMLSRYVVTCCAADATVVQVRVVNVTPGLFGPDDWIEVRGQIYPIGREVI
                     VVASSIEEVPRPDRPYLTP"
     gene            11454..13088
                     /locus_tag="VF195_07345"
     CDS             11454..13088
                     /locus_tag="VF195_07345"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6580669.1"
                     /translation="MPTMQRVLRASAALAFAALLAAACVGGGTGQSPPPSPEDPVSPE
                     PTRSAPPQPTAADGVQGAPDLPDDPDPDAVPADGRIDVSSLLDPPTLQPPPDRSRPFD
                     PGSGIFNLDHLIFIVLENRSFDHYFGTYPGADGLPRDANGRIDVCQPDPATGVCHRPY
                     HDTNFFDAGGPHGELGSHISIAGGEMDGFVEALRKIGNGCIKPHNDDFPPCVMATPGP
                     QGQPDVMGYHTGKELPIYWKYARTFTLHDRMFAPTDSWTLPAHLFLVSAWSATCTDLD
                     DPMSCSSDQHNPGHGPTNPSSRWSPPQGAPRPYVWAPITWLLYRAGIDWRYYVGEGTC
                     IAPPCIELKGTMTAAVQNPLPGFAATVATGQLDHVRSNKEFMQDARSGNLPPVSWVMP
                     VVDRGEHPPDSIEVGQAYVARLINSVMEGPREQWLRTAIFLTWDDWGGFYDHVEPPVV
                     DENGWGLRVPSLLISPWAKADHISHQTLSYDAYLKLIEDRFLAGERLDPQTSGWPDSR
                     PTVREEVGILGDLAMDFDFTQDPLPPLPLDPWPFRG"
     gene            complement(13131..13736)
                     /locus_tag="VF195_07350"
     CDS             complement(13131..13736)
                     /locus_tag="VF195_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010044715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX6580670.1"
                     /translation="MAGELEDIGTRLREERDRVQISQRELARRIGLSASMISQIESGQ
                     SKPSVSTLYAIVTELGVSVDDIFTGGNGSTAGPGDDGAAPGGSGHGAMVRLEDRHVID
                     LDSGVRWERLTSHEHEDVDFLHVVYDVGGSSASDEKMMRHPGREYGVVLEGRLGVRLR
                     FEEYALGPGDSIAFDSTQPHRLWNLGDVPVSGIWFVVGRDG"
     gene            13964..15292
                     /locus_tag="VF195_07355"
     CDS             13964..15292
                     /locus_tag="VF195_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6580671.1"
                     /translation="MTRILLKGGCVLTLGARSPNLRLGDVLIEDTVVAEVGVGLRARD
                     AEHVDATDTIVMPGFVDTHRHAWTSLFRNLGDHVSDGSAVEPARLGDHFRSEDIYAAT
                     LIGLLGAAEAGITTVVDWSHLRVDDGLARAALQAHADAGLRSVFVIVPDGARDRDATS
                     ATRQHVARLHDAAGPLTTIAFGAVLPEATDLGGVAEAWALARELGVRIHVHGRSELSG
                     RGVITALAERKLLGDDVTLVHRSELDDADLDAIGSSGASVSLSPSSEMASGPGSPPIQ
                     RLIDHGIRPGLGIDDERVAPGDMFAQMRATISLQHATVFDRKLAGKAGLPRLMSTRDV
                     IRFATVDGARAAGVGRETGSIEPGMQADVILLRTDRPNIFPINDPIGAVVWGMDTSNV
                     DRVFVAGRPVMRGGTLEANTEHARDLATAARRRVATAAGLATVAAQGVER"
     gene            15289..16362
                     /locus_tag="VF195_07360"
     CDS             15289..16362
                     /locus_tag="VF195_07360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580672.1"
                     /translation="MRATTKAPSAALTSFVVGSRYMPVYIALLLLVIVASIWAPATVS
                     GVALRAIAPYGAVLGITALGQMLVIMTGGIDLSVPGTMSLAAVIMVGVGDGSNERIWL
                     AIATTLAAAAVVGLVNGILIGGLRLNPLIVTLAVGLIVIGVVNRYGSTFPVQSPVPKG
                     FSAWTSTRILGVSPIFWIGVGLTVLLIVGIRYTAVGRRFQAVGANPVASHVVGVRVNV
                     HQILVFVVAAVCYAIAGAALAGLLRTPGVAVGSQYLLGPIAAVVIGGASLTGGLASPL
                     STFAAAIFLTGLTQMLRTMGLPTALQFVVFGLVIIGGMLVSGDRIVRGVEHLFRERRR
                     SDRSQGAVLSGSAGGPTWKEEGM"
     gene            16468..17775
                     /locus_tag="VF195_07365"
     CDS             16468..17775
                     /locus_tag="VF195_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX6580673.1"
                     /translation="MKRIRIIGVIAVLALVTAACGGDDTPSGEPTPTDATGGALEDLG
                     ESTDADPALIDKALGPVEPSDEASWNLILASVARAEQDLDQATIDKAMECWSQQECDT
                     GSGGETVMGYADGGGDTVNVWRAVSHMEAILQALTYDEIGSIVSTEADFNPDPAVHAN
                     DIRFLISKGVDFIVGYPDVGVALAEAVREADAAGIPYVSFSAGWVGLPDQEGALVPGE
                     DYLTVVGEDLCDLGNSFAEVLNEGVGDGEVAVLGGTPGNALSLGWQRCTVSSLASGLE
                     LVNPPANENSTTGDTSWYPPAIPGVYQGLLTTNPGIKGWAYEYADGMYIGLQTYEDLG
                     IPVKNLTVALRTDEQDLFCDWADRNEPTYNIWYSAGGNFQSRVGVTAAMVSLKGGEIP
                     AEVVVPHVMRQVTADDCDPDRVAPAVSGTSLVPDEVLQLMFAS"
     gene            17789..19384
                     /locus_tag="VF195_07370"
     CDS             17789..19384
                     /locus_tag="VF195_07370"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX6580674.1"
                     /translation="MGEGDRGGGAGPEGPVLELSGVSRQFGAVRALTDVSFDCRAGEV
                     HAVVGENGSGKSTLLGIASGFVDPDLGTVRIGGEPLRRDSPALARKLGLAMAYQDTSL
                     ILPEPVKNNLFLAAPEDQRPPFRRRKRWARKLLAEFDLDLELFPDAPAGSLSLADRQM
                     FEVAKALVTDPKVLLLDEPTTALGPDEVVALHRTVAACRRRGVGVVYVSHRLPEVLEI
                     ADRITILRDGRNRGTFDAKATNEADLVELIVGRPFEAAFPPPAARVGDAREVLVVDGL
                     QGQAFGPVSFTLAAGEIVGIAGAEGNGQPQLFDCLAGRQPPRAGRVVCDGRELSLIST
                     HEAVGAGMMLLPGDRIREALMPLLGARVNTTIQSLRRFSVLGILRRRAERRAVTDLVE
                     RLEIRTPSLEQPVGFLSGGNQQKVSVSRSFLKEPSVILAYEPTQGVDVSSRFDVYKAL
                     RTRTDKGTAILVKSSDPIELAGMCDRVLVLSRGQIIEEIPGDELDELRIVEAVVRGPG
                     LSKAGRSPLGVAMPKASSANTGQ"
     gene            19381..21834
                     /locus_tag="VF195_07375"
     CDS             19381..21834
                     /locus_tag="VF195_07375"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6580675.1"
                     /translation="MSERTNAARRAVRKVLRDARDRNKWRKIVRFRLWMPVVLQVVLI
                     ASLLWYTTSRFPGFINTTNVSQILILALPLIVAAMAQNHALLVGYLDLSVGGMISLGV
                     VIASFLIGAEASVSEILIGIGVVLLCGVALGLVNAGLVRGLKVPSIIATLATLSILDG
                     ISLTLRPTAQGVISPDLVTVLKTSIGPIPVAFIVVVAAAALADLWLHASGSGLALRAV
                     GFDERSAKRGGVGTNWLRVRALLLSAVLAAVASVFVMARSPIGNAQVGSTFALNSITA
                     AVLGGASLAGGRTTFIGGTVAAILLALILTVLPYLGLSPNDGPMIIGVLVLIGIVLFQ
                     LGDIKELAKRNYKRARRLMIGSRPPKAASLPAFYPAGTDFSVAPSGRTLIRGGTVLSL
                     DPALGDFATGDVLIEGETILAVGPDLTDGEVETIDASGMIVMPGFVDSHRHIWEGLLR
                     NIGTDVPLEGRSSYISFVLHKLAPAFRPEDAYIGDLVSALGAIDAGVTTLLDWSHIQG
                     SPAHTDAVIQALKDSGLRAVFAYGFPWWGKWEERQPSWFVRAATEHFSTKDQMLTLAL
                     AAPGPEFTDFEVSRDHWKLAREADARITTHVGVGSYGQDAKVQEMGEAGLLGPDTTYI
                     HCTTLNDTEIQMIVDTGGTVSLASPVEMMMGHGMPPIQKFLDRGLRPSLSVDVETNVP
                     ADMFNQMRSVLGLQRAVATAAGKSPLSAAEVLACATIEGARANGLDGVAGSLTPGKKA
                     DVVLLRTDRMNVTPLNDPATAVVTGMDTGNVDTVLIGGRVMKRHGELLHVDWPAVRRM
                     AEESRDFVVSRSGFKLPGI"
     gene            22013..23275
                     /locus_tag="VF195_07380"
     CDS             22013..23275
                     /locus_tag="VF195_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX6580676.1"
                     /translation="MPTTDGFPVATTPAPGIAGVDWEVRVDFERLRTYRLDRAKDALE
                     ASELGALLLFETSNIRYVTGTQIGYWAFNKGERYALLTRTGRPRIFDFGSAAKAHRLQ
                     LPHLYDETNSVGGNTGLQGAIHPRVGLQARAVQEIRSIMTEDGVGDMPLGVDVAETSI
                     FLELMQAGIEVRDGQQVMADAREIKSPDELMLLTQACAMVDGVYQDIFEALKPGVRES
                     DIVGLAHARLFEMGSEFVEAINSIAGERCSPHPHVFSDRLIRPGDQAYFDIIHVFNGY
                     RTCYYRTFVVGRATQAQRDAFARSREWMDAAIDLVKPGVATDQIAAVWPKAEDFGFAD
                     EMDAFGLQFGHGVGVSLHERPIITRLNSFVDPLEIKEGMLFALETYCPAADGRSAARI
                     EEEVIVTADGCRVITLFPCDELMVANEY"
     gene            23304..>23801
                     /locus_tag="VF195_07385"
     CDS             23304..>23801
                     /locus_tag="VF195_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020145554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6580677.1"
                     /translation="MPNTDTAEGTEIADLEARLSIYRMMQEGRLFEERARDLFLQGLV
                     KGTSHLGIGQEAIAAGFAAALEPDDLVFCTYRGHVHTLLRGAPMAALMAELLGRATGI
                     CGGKGGSMHLTDVSHGAMGSYAIVGAHMPIAAGAAWAARVRGTGQVTVCFFGDGTTNI
                     GSFHEA"
BASE COUNT         3596 a         8294 c         8256 g         3655 t
ORIGIN      
        1 accgtgtgcc acttgctggc gatctcgttc cctcgggcga tgtgccaccc cgcgaagttc
       61 gacgacccga cgtagaggat cttgccctcg ctcttgagct gatccatcgc ctcccagatc
      121 tcgttccacc acgcgttccg atcgatgtgg tgcatctgat agatgtcgag gtagtcggtc
      181 tgcagacgcc ggagcgagcc ctcacatgcc cgacggatcg cgagcttcga cagtcgtgac
      241 tcgttcggcc actcggccat ctgcccgtag accttggtgg ccagcacgac cttctcccgc
      301 cggccgccac ctttcgcgaa ccaatcgccg atgatccgct ctgtcgagcc cacgccgaga
      361 tgccgcccgt acacgttggc cgtgtcgaag aagttgatgc cctcggcgat cgccgcgtcc
      421 atgatgcgat gcgactcgtc ctccggcgtg tggggcccga agttcatcgt gccgaggcag
      481 agacgagaga ccaccagccc gagccgaccg acgtgcgtgt actccacggg ccgagtctac
      541 gcaggtgtga cggaccgctc tgcggccgcg agcgatccgt cccgctccgg gtcccgggcc
      601 ccctgcacga acatgaacac gagggtgatc gttccgccga tggcgccggc ccagatcagc
      661 gtcgcgtcgt gaccgatcag ctcggcggtg ggccccgtga tcgcgaacga gagcgggacc
      721 cccatcgtgg agatcatcca gtcgagcgcg aagacccgtc cgaggagccg gtccgggacg
      781 agccgctgca cgagcgtgac ccacacgacg acgaggtacg tgatcgacgc ctccgtgatc
      841 atcgcgaccc ccatcgcctg ccagacgccc gtgacgaccc cgaagcccgc ggtcccgaac
      901 atcccgagcg cccacgccag gtacatcgcc gtcagcggct tccgcgggag cctcccccgt
      961 tggccgaacg cggctgcggc gatgacggca ccgaacccgc cggcgccgaa gacgaggccg
     1021 agcgcccatg ccttgccccc gaggtcgttc ttcaccacca ccggcacgag ggtctcccac
     1081 ggaccccaca ccgccagcag gctcaccgtc gcggcgacca tcgcgatcag gagccagcgt
     1141 tgctccttga cgtagcggag tccctccccc acatcggcga ggaacgaggc ctgttcctcc
     1201 tgcccgttcc tcggcacccg ccgggtccgc atcgacagga tcatcaccgc cgagaacgcg
     1261 aacgtcgccg cgtccacgag gaaggccaat ccggcgcggt cgcctgccac cgagatcagg
     1321 acgccgccca cgagcggacc gacgagcgtc atcgcgaacg gtcgcacgag ttgggccaga
     1381 gagttggcct ggacgagcag atcggccggc acgatagcgg gcgtgatcga cgagaacgcc
     1441 ggctggaaga cggcttgtcc cgctccgtac acggtgacca gcgcgaggat gatcgggatg
     1501 gagtccagcc gatcggtgat cgacagcacc cccagcgtgc cgatcgcgac caaccggatc
     1561 gcgtcgccgg cgatcatcag gtgacgccgc tcgagccggt ccgacagcgc gccgctcgcg
     1621 agcacgagca ccacctgggg gagcgaccag gcgacaccga cgagggcgag cgcgcccggc
     1681 cggttctcga tcgacaggac ctcccaggcg atcgagacga ggtagatccc gtccccgatc
     1741 atcgagacgg tcatccccgt ccacaggaac gcgaagtcgc ggacggcgag gggcttgagg
     1801 atctcgatgc gatcgcggaa ggaggagggt cggtcgctca cgatcctcgc agcataggag
     1861 gcgacgagga cccggctcgg gcccggaccg ctaccgccag tcgacggtga cgacctcgat
     1921 cggatccgag atccccttca cggtcacggt ccggggatcc gccgtgggga actgcccgcc
     1981 gaccgccgtc tcggcgcttg cgacgatctg gctcccctcc gccaagccgc cgatccgtga
     2041 cgcctcgtgg acccccttgc cggtgaagtt ccgtccgacc atcgtcgcgt cggaggcgtg
     2101 gaggccgatc cgcacctgcg gcgcgaagcc cgcagagttg cgatgctccg cgagcccccg
     2161 ctggatcgcg accgcgcagg ccagcgcgtc gtcgggcgac tcgaacgcca cgaagaaccc
     2221 gtccccggtc gccacgacct cctcaccgcg gttggccccg aacagcgcgc gcaaggtctt
     2281 gtcgtgccac gagaggagcg actgccacgc ctcgtccccc atcgcctcga ggaggctcgt
     2341 cgaccccacg atgtcggtga acatgaacgt gcgctgcacg cgctgggtcg gcgaacggcc
     2401 ccggagctgc cacgggagga gctccgcgac ctcctcgagg acgatcgcct gcgtggggca
     2461 ggcgagggca gcctcgcgga gcttctcctc gtccacgctc gcggcgtcga ccacgccggc
     2521 cttgccgaag tcgccccggt gccagtcgaa ggccgtgggc gcgatcgtga tgcagttccc
     2581 ggcgccgatg caccgcggtc ggtccaccct cgcccggagc actagaggat ccccgactgg
     2641 aacgcgaacg ccgtcgcgtc ggcgcgcgat gaggcgccga tgctcgcgta catcgatgcg
     2701 aggcgccggg tgatctcgcc gggatcgacg tcgagacggt cggcgatctc ctcgttcgag
     2761 agaccctgga cgacgagctt gaggaggtcc gcgtcgtcgg agccgagtcc gtcgacaccc
     2821 acgtccttgg cggtgcggac gatcggcgca tcggggtcca acgccagccc ggcgagggcg
     2881 ctgcccgtcg gacctcggaa ccaccggctg cggatccctt cgtcgacgat gcggttcgcg
     2941 atcaccgccg cctgcagccg caggtacgaa acggtccccg cccgctcctc gtcggcaccg
     3001 gccgcggtga tcgcggcggc gacgggaacg agcacgtcga ggttgaggtc ctcgtgctgc
     3061 gccgctcgaa gcgcttcgac ggccgatcgg gcggcctcca ccgcacggtc cggctccccg
     3121 cgcgagagcg ccaccgccgc gagcgcggag tcggccttcg cgccccacgg ggcatggccc
     3181 ggcagcaacg ggaggaactc cttcgcgctc gcggccgacc gctcggcgag cgaccgcagc
     3241 tcctcgtcct cgcgctcggc gccgagggac gcagccgcga gcgcgagcgc cgtcagcgct
     3301 tcgcaccgcg ctgcggggag tccctggtcg gcggccatct ggaccgcgcg ctcgagatgc
     3361 tcgcgcatcc cggcggcgtc accgcgcgcg gcatccgtca gtccgcgcca cagctccagc
     3421 tgccgaccgc gtgcaggcgt cggactctcg agcgcgacgg tggcggcgcg atcgagccag
     3481 gtgatcgcct cgtccaggtc gccgagctcc acgttcgaca tcgccgtgcc gcctgcggcg
     3541 aggaactcga gctgccggtc gccgatcgcg cgggcgcgct cgtaggcctc ggcgccgcgg
     3601 gacagggcga ggtccgggat caccttcgcg cgcgcgaaca cgtggacgcc gtagagcatc
     3661 tggacctcgg cgaacgcccg ctggctctcg ctcgtgaacg acgccagctg tgtcgcgagc
     3721 cggcggatct cctcgatgtg gcgggccgag tccgcgccga tgtggatgtc gggcgcccag
     3781 ctcaggtagg ccatcgcgat gatcgtcgcc atcgtcccct ggcgatcgcc gagctcctcg
     3841 aagagggcga gcgcgcgatg cagacgctcg cccgaggaac gcacccacgg cgcgaccggg
     3901 agctcctcga cgaggtcatc gagcgattcg ccggccgcca cccgttccat caccggcaga
     3961 tgcgcgccga ccagcacctg ttcgacgaac caaccgcgca cgcgaccgag gtcgatcacg
     4021 cccagctcgc gggtcgcggc ggccagcatc tgccgatcgc cgagctcctc cgcgagcacg
     4081 accgcccgcg cgtacgcctc ctcggcagca tcgaggtcca cctcggccgc cgatggcgtg
     4141 aagccctccc ccaccccggc gtggagcagg gcctggccga gcgcgatgca cgcggccagc
     4201 tcggaggggc ggtcaccgcg ctcgacggcg agcgttcgca cgcgcgccgc gagctcggcg
     4261 gcgcgatcct cctgctgcga gagacggagc gcggccgagc gacggagcgt gacgtccagg
     4321 tccacggcgg ggtcgccgag cgcctccgcg agcgcggcca gctccgacaa cccctcgaga
     4381 cggtccgccg gccggcgcag catcgcgagg gcatcatccc gagcggtcag caggccgagc
     4441 cgctcctgcg gggtcgcggc ctgcggcagc gcgatctcca ccacccggag gacctcgtcc
     4501 ggggcgtgcg cggcgagtgc ggctgccgcg gcctcggtcg agaagcggac gcagacctgc
     4561 gcgtcgcccg cagcccgggc gtgatgggcg aggagcggca gcgtctcggg cgccggctcg
     4621 ccggtcagca ggagctggac gatagcggca tggatctcgc gccggcgagc cggcgccaac
     4681 gtgtcggcgg cgaactcgcg gacctgttcg tgagggaacg tgtagtcggc cggcgagtcc
     4741 tcgggctgct ccacgagcag tccggccgcg accgcgggtg ccatcaggtc accgagcgag
     4801 accagatccc cgtcgggccc gccctcgccg atccgcaggc ggacctcccg gaggtccttc
     4861 aggctgaagt tccgccccat cagcgccgcc tcggcgagcg ccgtcttcga ctccaccggg
     4921 aggtgcgccg cccgccgctg gatcaacgtg cggacggccg acggtgccag ccgctcggcg
     4981 ttgcgtccca gggtccacgc gtccgcggcc tgctgcagca tccccgcctc gcgataggcg
     5041 cgagcgatct cctccacgat gaacggcacg ccctcggcct ggccgtggat cgtcgaggcg
     5101 gtggccggct cgacctgtcc gccgaggatc tggcgcagga actccgaggt ctccacgacg
     5161 gagaagcgag cgagcttcag gcgccggatc acgcgcatcc gctccatgtc ggcgaccagc
     5221 gtgaccgcct cggtgagcgt ggcgagctcc tccgggcgga ccgagaacat cacgaagatc
     5281 gggctcgcgc cctcggtgcg gatcacgtag cggagcaggc ggaggctgtc ctcgtccgcc
     5341 cactgcatgt cgtcgatcaa cagcgcgagc ggctgcgcgg ccgtgagcgc gcggacggcc
     5401 atcgtggcca ggtcgaacga tcggagcagc cgctgatcgg cggacatgcc ggcgaacgca
     5461 gggtcgtcac gtccgccgag cgccgagctc gccgcgatga tcgcctgctc cgacggcgag
     5521 ccgctcaccg cttccatcac ctccggcgcc ccgacgatcg agcgtgccag gaggaacggt
     5581 ccgcggagct cctcgtccgc gcccacggcg atcgtgagga acccgcgcgc gccggccatc
     5641 tcccgggccg ccaggaggag ccgcgtcttg ccgatcccgg gttcgccctc gacggtcagc
     5701 gcggtcaggc gtccaccctc cgcgccgccg atcgcatcct cgatcgcggc gagctcggcc
     5761 ggccggccga cgaccgcgcg ctcggtgcga tgttcgaaga ggcccagcgg ggcgaacgcg
     5821 gccgcggcga cgggaggcgc ttcgatcatg cgggaccctc gagcgaagaa cggcactggt
     5881 cggtcatccg gggattatcc ggcgcgagga gggtccctgc tatgctcggg tccccgagcc
     5941 gatcggggag atcccgcatc gagcgcctct ggtggcatcc ctgacccacg tgccctcgtc
     6001 ctgcggatcc ggcgaaccac gacgagaggc aggccgcgtg gaagggatca accgcaagga
     6061 tcgcgtcttc ggtcctcccg aacgaggcaa gaaggcagcc gatcggccgc cgcgaggacg
     6121 gatgtgtgtg cacatcgggt gcgagacgct cctctccacg tacaactcat ccgacgtgtg
     6181 ctggctccac gccccacccg cctaccgcca tgccctgagt cgcgactagg gcggtagcat
     6241 ccggctggat gcgaaccctc cgggcggtca cggctgcgct gttgctcgcc ctgctgcact
     6301 cgggcagcgc agcgtgtacg agctcgaacg gcggaccctc cgtttccgcg tcgccttcac
     6361 tggatgtcgc gcccggtggg tacgtctacc gatcggagga gatcgaggcc acgatcgaga
     6421 tcacgggcga agcgggaacg ctcaccgtct tcaacgggac cggcttcccc ctcgatgacc
     6481 ccggggtgta tctgctggac gcgcgcgacg gctctcgggt cgacgcagag gtgatcgacg
     6541 cggctcccgt ccccgacgga gagacgggtg agttcgaggt cacgatcccc ggcgcccccg
     6601 aagcgaagca cgtcggcctg atcgtcctgc tcgcaggcga cgccgatctc ggcgcgttcg
     6661 tccccgcggg ggatcaggca tgaatcggac ggggatcacg atcgccgcgg tgctcgcgct
     6721 caccgcgctc gtcgtccagg acgggacggc tctctcccag gaggacgccc aggagcaacg
     6781 cttgatcgac accgtctgcg cgatcccgcc ggtgctgctg gagcgcacgc tccacggcca
     6841 ccgtgaggac cgcgccggcg agatccagct cctgacacgg gaacccgact tcgtcggtca
     6901 cggcggcttg ccccactcgg gtccgtggga ctacgtggga caggtgccgc tgctctggta
     6961 cggcccgggc tatgtgccgg cgaacggacc gatcgaccgg cgtgtcacgc tggccgacct
     7021 cgcccccacc caggccgagc tgctggggtt cgacttcgac gcccctgacg gcaagccgct
     7081 gctcgacgcg ctggtccccc gggaggaccg tccggaaccg ccggccctgc tcgtggtgct
     7141 cgtatgggac ggtgccggga acaacgtgct gaaggcacat cccgacgcgt ggcccaacct
     7201 ccgagagttg atggacgagg gcgtctggta taggcgcgcg gaggtcgggt cgtcgccgcc
     7261 gagcaccgcg cagatccacg ccaccatcgg gaccggcgcg ttctccgcga cccatgggtt
     7321 gatcgcgcat cacttccgga tcggcgatcg gatcgtcacc ccgtggtcgg caggtccccg
     7381 ctacttggtg cgtcccacgc tcgccgacct ctacgaccgc gccctcggga accgcccgct
     7441 gatcggagcg acggcgacgg tcgcgatcca cctggggatg ctcggtcacg gctcgttgtg
     7501 ggggggcggc gaccgcgacc tcgcggtgct ccgcgagcga gagggagcct ccacgctcgg
     7561 cgccgaaggc atccgctgga acctcacctc gagcgtggca cccttctacc atctccccgg
     7621 gtacgtgaac gacctgccgc ctctgtccac ctacaccgaa gacgtcgatc gcgcggacgg
     7681 acggctcgac ggcaagtggc gcgacgtcgt gatcgagagc gaggccgtgc acttcgggtt
     7741 gctgacaccg gccaggatcc ctttccagga tcgcctcatc gaggaactga tcgcgaccga
     7801 aggattcggg gacgacgatg tgtccgatct cttctacatc aacaagaaac tgatcgacga
     7861 ggtaggccac atcttctcga tgaacagcgt ggagatggag gattcgatcc gcgccgagga
     7921 cgccgccctg cccgggctga tcgagatgct gaaccgcgag gtgggcgagg gtcgatgggt
     7981 catggcactc acggccgacc acggccacac acccgatccg gcggtgagcg gcgcggacgt
     8041 gatctcaccg accgacgtcg gtcggcagat cacggagggc ttcgaccgcg acggtgacga
     8101 cgtctcggtg ctcgagtaca cgcagcccac ggaggtcttc atcaacgagc gcgagctggc
     8161 ggacgagggg cacacgctgg ccgacgtcgc cgactacctc atgggactca cgaagggcga
     8221 tgtcggcggt gacatctggc cggtcccgga ggagcatcgg gccgagccgg cgttcctcgc
     8281 ggcgtatccg tctgagctcc tcgatcgtct gccgtgcctc ccgcccggcg agggctgagc
     8341 cgagcatgcg atgacccggc gggccgcgat cctcctggtg gtggcagcga tcgtcgccgc
     8401 gccgggcatc gtgctccagg cgacctgccc cggtgactcg tgcgcacgat ccggcccggc
     8461 gcgcacgccg ttctgctccc tcccggagga gctccggacg aggatcgcgg ccgggtatcg
     8521 cgagggaagg tcgccggatg tcctcgccgt caccggaggg accggcgtcg tcggttcggt
     8581 gccggcggtc ggtgcaccca ccacggtcgg gagctggccg tccacgtcgt caccgatcga
     8641 cacgaggacc cccatcgtgt tccggggcgc cggggtacgc ggagcctcga gcgtccccgc
     8701 cggaacggag ctgcgccaga tcgcgccgac gatcgcgcag atcctccgat tcgatcgacc
     8761 gttccccggg gtccgttccg ggcggccgat accgggcatc gccggacccg acgtcccacg
     8821 gctcgtcctg gcgatcgtct ggaagggagt cggcacggcc gacctggaga acgatcccga
     8881 tgcctggccc ttcctgcgcg ggctggtgtc gtcgggaaca gggacgctcg acggggaggt
     8941 cggatcgctc ccgatggacc cctcggcagg attgacgacg atcgggacgg ggggcctccc
     9001 ctccgagcac ggcatcacgg gcggcttgtt caggggcgac cgcgccgagg tcgtcgcggc
     9061 ctgggccgct ggcgctccga ccagcgtcat cgcgacgctc cccgacgacc tggatcgcgc
     9121 gttcgcacag cgtcccctga tcgggatcgt cggctcggat cccgccgatc gcgggatcgt
     9181 cgggagcggc tggtacgccg agcgcgatcg tgacgatcgg gtgacggccg tcgctggtca
     9241 ggcccgactg cgcgccgtca atagactgct cgcgagcggc tacgggcggg acgacgtacc
     9301 ggatgtcctc gcggtggtga tggagggaag cgtgggctca ctcgacgcag agctgcggcg
     9361 caccgtgcga gccgcacggt ccgccaccgc cgggcaggtc ctgatagtgg tgaccgccac
     9421 aggcgccggg acggctcccg gatcgatgca gatcgaggcg gcggacgtcg agcggatcgt
     9481 cgaggccgag atcgccacgg acgagccact ggtccaggcc gtcgtccccg ccggcttcta
     9541 cctcgatcaa cgggcgatga cggcggccgg cgtgacgggc cagatcgccc aggacgccgc
     9601 cctggcggct cgcgggcctg acggatcggc gatcttcgtc gacgccttcc agggcttcgc
     9661 cgtccccttc gggaggtact gctgatgacg gagctcgtcg cgacacccag cggtcgccgg
     9721 cccatctccc cacggctcgc ggggctgatg atcgtcgcgt tggtcctcct cggggcgtcg
     9781 atacgactgt tcgggcccac acgggtggat tggctccggg cagcgctcgt cgtcttcggg
     9841 tcgctcgtga tccaggcggt ccccttcgtc ctcgtcgggg ccaccgcggc cgctctgatc
     9901 gaggtcttcg ttccgatcgg agccttcgag cggatcgcca ggctccctcg gccgctgcgg
     9961 ctcccggctg ccggcctggc gggcgtcctg ctcccgatct gcgagtgcgg atccgtaccg
    10021 gtcgcccgac gactcatcca caggggtctc gacccgtcgt cggcgttcac gttcatgctg
    10081 gcggcgccgg tggtgaaccc ggtcgtgatc gcgtcgacct tctacgcgtt ccggggcagg
    10141 gatacgctct ggaccatggt gctcggtcgc accggtctcg ggttgctcgt ggcgatggcc
    10201 gtcgggtggg ccttgggggc gctggggaag gagcaggtcc tgcggccggt cgatgaggag
    10261 gatcgagggc acagcctcga gctcgggagg cccgagccac ggtggcgaag gttcttcgtg
    10321 cacctcgccg acgacttcct cttcatgggc ggctacctgt tgctcggcgc ctcgatggcc
    10381 gcgatcatcc agacgtttct cccgcagggg ctcctcgagc gcatcggggg aacaccggtc
    10441 gtcgacatcg tgctgatgat ggggatcgcc tcgctgctgt cgctctgctc cgagtcggac
    10501 gctttcatcg cggcgtcgtt cgcgcagttc ggcgcgtcct cccagctcgc cttcctggtg
    10561 ttcgggccga tggtcgatct caagctcggc gcgctctacg ccggcagctt ccggcgggtg
    10621 gtcgtgcaga cgatcgtcgc ggtggccgcg gtgaccacgc tcatcggctc gatctggatc
    10681 gaggtgttga ccggatgagc ggccggtcct tcagccaacg tcggctcgcg gcggcctcgg
    10741 tgctcgccgc gtggtcggga ctcttctggt tcctcatcat cacggaccgg gtcgacctct
    10801 acctctccac gaggacctcg tgggtggtcc cgatggccgc gggaggcctg gccgccgtgg
    10861 cgctcgggct ggtcctcgcc gggcgttcct cgaggccgga gcccgtccgg gtccgagaag
    10921 cggtcatcat gacgctgctg gtcacgcccg tcgtcctcct ggtcaccctc cccccttcga
    10981 cgctcggcag cttctcggcg ccgaagaagg cgcagttctc cggctccgca ctgtcgacga
    11041 tctacggtcg gttcgacgag acgagcgaga tcacgatgct caccgtcgcc gccggcgaga
    11101 cgtccgacga gggctcgcgc ctgctcgcga cccgggccgg cgcggaggtc ggcttcgtcg
    11161 ggttcgtcgc gcgtgacatg agcgccgagg ccgacgagct gatgctgtcc cgctacgtcg
    11221 tgacctgctg cgcggccgac gcgacggtgg tgcaggtgcg ggtcgtcaac gtcacgccgg
    11281 ggctgttcgg accggacgac tggatcgagg tcagggggca gatctacccg atcggtcgcg
    11341 aggtcatcgt ggtcgcgagc tcgatcgagg aggtcccccg acccgatcgg ccgtacctca
    11401 ctccttaggg atcggacccg ccgggaaggc acccgccgat aggatgcctg gtcatgccca
    11461 ccatgcagcg cgtcctgcgc gcctcggcgg ccctcgcctt cgccgccctg ctcgccgcgg
    11521 cctgcgtggg agggggcacc ggtcagtcac ctcccccgtc ccccgaagac ccggtgtcgc
    11581 ccgagccgac acggtccgca ccgccccagc cgaccgcggc cgacggggtc cagggtgcgc
    11641 cggacctccc cgacgacccg gaccccgacg cggtgccggc ggacggccgg atcgacgttt
    11701 cttcgctcct ggaccctccg acgttgcagc cgccaccgga ccgatcgcgg ccgttcgatc
    11761 ccggatccgg gatcttcaac ctcgaccatc tcatcttcat cgtgctggag aatcgctcct
    11821 tcgatcacta cttcggcacg tacccgggtg ccgacggact cccgcgtgac gcgaacggcc
    11881 ggatcgacgt gtgccagccg gaccccgcaa cgggcgtgtg tcaccggccc taccacgaca
    11941 ccaacttctt cgatgcgggt ggcccgcacg gcgagctggg atcgcacatc tcgatcgccg
    12001 gtggggagat ggacggattc gtcgaagcgc tgcggaagat cggcaacgga tgcatcaagc
    12061 cgcacaacga cgacttccca ccgtgcgtca tggcaacgcc cgggccgcaa ggacagcccg
    12121 acgtgatggg ctaccacacg gggaaggaac tcccgatcta ctggaagtac gcgcggacct
    12181 tcaccctgca cgaccggatg ttcgcgccga ccgattcctg gaccctgccg gcgcatctgt
    12241 tcctggtctc cgcgtggtcg gcgacctgca cggacctcga tgacccgatg agctgctcct
    12301 cggaccagca caaccccggg cacggcccga cgaacccgag ctcgcggtgg agcccgccgc
    12361 agggcgcacc tcgcccgtac gtctgggcgc cgatcacctg gttgctgtac cgcgcgggca
    12421 tcgactggcg ctactacgtg ggtgagggca cgtgcatcgc tcccccgtgc atcgagctga
    12481 aggggaccat gacggcggcg gtgcagaatc cgctgccggg cttcgcggcc accgtggcca
    12541 cgggccagct cgaccacgtc cgctcgaaca aggagttcat gcaggacgcg cgctccggca
    12601 acctcccgcc ggtctcgtgg gtgatgcccg tcgtcgaccg gggcgagcat ccgccggact
    12661 cgatcgaggt ggggcaggcc tacgtcgcgc gtctgatcaa ttcggtgatg gaaggacccc
    12721 gcgagcagtg gctcaggacc gcgatcttcc tcacctggga cgactggggc ggcttctacg
    12781 accacgtgga gccgcccgtg gtcgacgaga acgggtgggg cctccgggtc ccgagcctcc
    12841 tgatcagccc gtgggcgaag gcggaccaca tctcccacca gaccctcagc tacgacgcgt
    12901 acctgaaact gatcgaggac cgcttcctcg ccggagagag gctcgatccg cagacgagcg
    12961 gctggccgga ttcccgtccc accgtccggg aggaggtcgg gatcctcggg gacctggcga
    13021 tggacttcga cttcacgcag gatccgctcc cgccactgcc gctcgacccg tggccgttcc
    13081 ggggatgacc ctgtcgagcg agcgaatcgc cggcccgagc cgagatcact tcagccgtcg
    13141 cggccgacga cgaaccagat cccgctgacg ggcacgtctc cgaggttcca cagccgatgg
    13201 ggctgcgtgg agtcgaaggc gatcgagtcg ccggggccga gcgcgtactc ctcgaagcgg
    13261 agtcggaccc cgagccgccc ttccagcacc acgccgtact cccgtccggg gtggcgcatc
    13321 atcttctcgt ccgaggccga ggaacccccc acgtcgtaga cgacgtgcag gaagtccacg
    13381 tcttcgtgct cgtggctcgt gagccgctcc caccgcaccc cggagtcgag gtcgatcacg
    13441 tgtcggtcct cgagcctgac catcgcgccg tgacccgatc cgccgggcgc cgctccgtcg
    13501 tcgccgggcc ccgccgttga gccgttgcca ccggtgaaga tgtcgtcgac cgagacaccg
    13561 agctccgtca cgatcgcgta gagcgtgctc accgagggct tggactgacc cgactcgatc
    13621 tgggagatca tgctcgccga cagaccgatc cggcgcgcca gctcgcgctg gctgatctgc
    13681 acgcgatccc gctcctcgcg gagcctcgtc ccgatgtctt cgagctctcc ggccaccccg
    13741 cacctcgccg cgttcggtga tcgcagcgta gcgcttagcg tgactgagga ctacccgtgg
    13801 gatcgaacgc ccggctccgg tattgcctag ccaggagcag gcgaggtatg tagtgtccct
    13861 gaacggtgac ggccggttcg cgaagtgaga gtgagcggac gacgccgacc cgggacggcc
    13921 gggacacccg gaccgtgggg cctgggtcgg cgcagggggg cggatgacga ggatcctgct
    13981 caagggtgga tgcgtcctca ccttgggcgc caggtcgccg aacctccggc tgggagacgt
    14041 cctgatcgag gacaccgtcg tcgccgaggt cggggtcggg ctccgcgccc gcgacgccga
    14101 gcacgtcgac gcgaccgaca cgatcgtgat gccgggcttc gtcgataccc accggcacgc
    14161 ctggacctcc ctcttccgga acctcggcga tcatgtgtcc gatggatcgg ccgtggagcc
    14221 ggcgcgtctc ggtgatcact tccggtccga ggacatctac gccgccacat tgatcggctt
    14281 gctcggggcg gcggaggcgg gcatcacgac cgtcgtcgac tggtcacacc tccgagtcga
    14341 tgacggcctc gcgcgcgccg cgctccaggc gcacgccgac gccgggctgc gatccgtgtt
    14401 cgtcatcgtc cccgacggcg cccgcgatcg cgacgctacg tcggcgacca ggcagcatgt
    14461 ggcccgcctc cacgacgcgg ccggaccgtt gaccacgatc gcgttcgggg cggtcctgcc
    14521 cgaggcgacc gatctcggag gggtcgccga ggcctgggca ctggctcggg agctgggcgt
    14581 gcggatccat gtccacggac gctcggagct ctcgggacgt ggtgtgatca ccgccctcgc
    14641 tgaaaggaag ctgctgggtg acgacgttac actcgtgcat cgctcagagc tcgacgacgc
    14701 cgacctcgac gccatcgggt cgtcgggagc atcggtgtcc ctgtcgccct cgagcgagat
    14761 ggcgagcggg cctggatccc cgccgatcca acggctgatc gatcacggca tccggcccgg
    14821 gttggggatc gatgacgaac gggtcgcgcc cggcgacatg ttcgcccaga tgcgggcgac
    14881 gatctccttg cagcacgcca ccgtcttcga tcgcaagctg gccgggaagg ccggcctccc
    14941 gcggctgatg agcacgcgcg acgtcatccg cttcgccacg gtcgacggcg ctcgggcggc
    15001 aggggtgggc cgggagaccg gatccatcga gccggggatg caggccgacg tcatcctgct
    15061 tcggaccgac cgccccaaca tcttcccgat caacgacccg atcggcgccg tcgtctgggg
    15121 catggacacg tccaacgtcg accgggtctt cgtggccggc agacccgtga tgcgcggcgg
    15181 caccctggaa gcgaacacgg agcacgcccg cgacctggcg acggcggccc ggcgccgcgt
    15241 cgcgaccgcc gccggtctcg ccacggtcgc cgcacaggga gtggagcggt gagggcgacg
    15301 acgaaggcac cctcggcagc gctcacctcg ttcgtcgtcg gctctcgcta catgcccgtg
    15361 tacatcgctc tgctcctgct cgtcatcgtg gcgtccatct gggctccggc aaccgtcagc
    15421 ggcgtcgccc tccgtgccat cgcgccgtac ggcgcggtgc tggggatcac cgctctcggc
    15481 cagatgttgg tgatcatgac cggcgggatc gacctcagcg tccccggaac gatgagcctg
    15541 gccgccgtga tcatggtcgg agtcggggac gggtcgaacg agcggatctg gctggcgatc
    15601 gccaccacgc tcgccgcagc cgccgtggtc ggactcgtca acggcatcct gatcggggga
    15661 ctcaggctga acccgctgat cgtcaccctg gcggtgggtc tgatcgttat cggcgtggtg
    15721 aatcgctacg gaagcacgtt cccggtccag agcccggtgc cgaagggatt ctccgcctgg
    15781 acctcgacgc gcatcctcgg tgtcagcccg atcttctgga tcggtgtcgg gctcaccgtc
    15841 ctcctgatcg tgggcatccg ctatacggcg gtcggccgca ggttccaggc ggtgggtgcc
    15901 aacccggtgg cctcgcacgt cgtaggcgtc cgcgtgaacg tgcaccagat cctggtcttc
    15961 gtggtcgccg cggtctgcta cgcgatcgcc ggggccgcgc tggcggggct cctccgaacc
    16021 cccggggtcg ccgtcggctc acagtacctc ctggggccga tcgcggcggt ggtgatcggt
    16081 ggcgcctccc tgaccggcgg gttggccagt ccgttgtcca cgttcgcggc cgccatcttc
    16141 ctcacgggcc tgacccagat gttgcgaacg atggggctgc ccaccgcgct gcagttcgtc
    16201 gtgttcggcc tcgtgatcat cgggggcatg ttggtctcgg gcgaccggat cgttcggggc
    16261 gtggagcatc tgttccgaga gcggagacga tcggatcgat cgcagggtgc ggtcctctca
    16321 ggctccgccg gaggaccgac ctggaaggag gagggtatgt gacagttcca tcgatctggt
    16381 ccatcgactt ggtccatcgc tttggcccag cgacccggga agtccagatc tgacaacgca
    16441 cgaacgatcg agagtgggag gaggagcatg aaacggatca ggatcatcgg cgtcatcgcc
    16501 gtgctggctc tggtcacagc ggcatgcgga ggtgacgaca cccccagcgg cgagccgacc
    16561 ccgaccgatg ccacgggggg tgcgctggag gatctcgggg aaagcaccga cgccgacccg
    16621 gcgttgatcg acaaggccct gggcccggtc gagccgtcgg atgaggcgag ctggaacctc
    16681 atcctggcgt cggtcgcgcg ggccgagcag gatctcgatc aagccaccat cgacaaagcg
    16741 atggagtgtt ggagccagca ggagtgtgac accggcagcg gcggcgagac ggtgatgggc
    16801 tacgctgacg gcggcggcga cacggtgaac gtctggcgcg cggtgagcca tatggaggcc
    16861 atcctccagg cactcaccta cgacgagatc gggtcgatcg tgtccacgga ggccgatttc
    16921 aacccggatc ccgccgtcca cgccaacgac atcaggttcc tgatctcgaa gggtgtcgac
    16981 ttcatcgtcg ggtacccgga cgtgggagtg gccctcgccg aggccgttcg ggaagccgac
    17041 gccgcgggca tcccctacgt ctcgttctcg gcagggtggg tcggtctgcc cgaccaagag
    17101 ggggccctcg ttcccggcga ggactacctg accgtggtcg gtgaggacct ctgcgatctc
    17161 ggcaacagct tcgccgaggt gctgaacgaa ggtgtcgggg acggcgaggt ggctgtcctg
    17221 ggcgggaccc ccggcaacgc gctgtccctc ggctggcaac ggtgcacggt ctcatcgctc
    17281 gccagcgggc tcgaactggt caaccccccc gcgaacgaga acagcacgac cggtgacacc
    17341 agttggtacc ccccggcgat ccccggtgtg taccaggggc tgctgacgac gaatccgggg
    17401 atcaagggct gggcctacga gtacgccgac gggatgtaca tcggcctgca gacgtatgag
    17461 gacctgggca tcccggtgaa gaacctcacc gtcgcgctgc gcaccgacga gcaggatctg
    17521 ttctgcgact gggcggaccg gaacgaaccg acctacaaca tctggtactc ggccggtggc
    17581 aacttccagt ctcgcgtcgg cgtgaccgcg gcgatggtgt cgctgaaggg cggcgagatc
    17641 ccggccgagg tcgtggtgcc gcacgtgatg cgacaggtga cggcggacga ctgcgatcca
    17701 gatcgtgtcg ccccggccgt ctccggtacg tcgctggtgc ccgacgaggt cctgcaactg
    17761 atgttcgcta gttgatgatc ggcaaccgat gggtgaaggc gatcgtggag gaggggcggg
    17821 ccccgagggc ccggtcctcg agctgagcgg cgtctcgcgg cagttcgggg cggttcgagc
    17881 gctgaccgat gtcagcttcg actgccgtgc cggcgaggtc cacgccgtgg tcggcgagaa
    17941 cgggtcgggg aagtcgaccc tcctcgggat cgcaagtggg ttcgtcgacc ccgatctggg
    18001 cacggtccgg atcgggggcg aacccctgcg ccgggattca ccggcgctcg caaggaagct
    18061 gggcctggcg atggcgtatc aggacacctc gctgatcctg cccgagcccg tgaagaacaa
    18121 cctcttcctc gcggcgcccg aagaccagcg gccgccgttc cggcggcgga agcggtgggc
    18181 gaggaagctg ctggccgagt tcgacctcga cctcgagctg ttccccgacg cgccggcggg
    18241 gtccctctcc ctcgccgacc gacagatgtt cgaggtggcc aaggcgctcg tcacggaccc
    18301 gaaggtgctg ttgctcgacg agccgaccac cgcgctgggg ccggatgagg tcgtcgcgct
    18361 ccatcggacc gtcgccgcct gcagacgtcg cggcgtcggc gtggtgtacg tgagccatcg
    18421 tctgccggag gtgctcgaga tcgcggaccg catcacgatc ctccgcgacg gcaggaaccg
    18481 gggcacgttc gatgcgaagg cgacgaacga agcggacctc gtcgagttga tcgtcggccg
    18541 cccgttcgag gccgcgttcc cgccccccgc cgctcgcgtg ggtgacgccc gtgaggtgct
    18601 cgtggtcgac ggcctccagg ggcaggcatt cgggcccgtc agcttcacgc tggcggccgg
    18661 cgagatcgtg gggatcgccg gggccgaggg gaacggccag ccccagctct tcgattgcct
    18721 ggcgggccga cagccgccga gggccggacg ggtggtctgc gacggccggg agctgtcgct
    18781 gatctccacc catgaggcgg tcggtgccgg gatgatgttg ctgcccggcg atcggatccg
    18841 cgaggcgttg atgcccctgc tcggcgccag ggtgaacacc acgatccagt cgttgcgtcg
    18901 gttctccgtg ctcggcatcc tgaggaggcg agcggagcgt cgcgcggtca ccgacctcgt
    18961 cgaacggctg gagatccgga ccccgtccct cgagcagccg gtcggcttcc tgtccggtgg
    19021 caaccagcag aaggtctcgg tgtcccgcag cttcctcaag gagccgtcgg tgatcctcgc
    19081 ctacgagccg acgcagggcg tcgacgtcag ctctcgcttc gacgtgtaca aggccctgcg
    19141 cacccgaacc gacaagggga cagcgatcct cgtcaagtcg agcgacccga tcgagctcgc
    19201 ggggatgtgc gaccgggtgc tcgtcctgtc ccggggccag atcatcgagg agataccggg
    19261 tgacgagctc gacgagctgc gcatcgtgga ggccgtggtc cgtggacccg ggctctccaa
    19321 ggcagggcgg tcgcctctcg gggtcgccat gccgaaggca tcctcggcga acacgggcca
    19381 atgagcgagc gaacgaacgc ggcacggcgc gcggtccgga aggtgctccg cgacgctcgc
    19441 gataggaaca agtggcgcaa gatcgtcagg ttccgtctgt ggatgccggt ggtcctgcag
    19501 gtcgtgctga tcgcatcact gctctggtac accacctcgc gattccccgg cttcatcaac
    19561 accaccaacg tctcgcagat cctgatcctg gcgctgcccc tcatcgtcgc ggcgatggcc
    19621 cagaaccacg ccctcctggt cggctacctc gacctctccg tgggcggcat gatcagcctc
    19681 ggggtggtca tcgcctcctt cttgatcggc gccgaggcct ccgtctcaga gatcctgatc
    19741 gggatcggcg tcgtcctgct ctgcggggtc gccctcggtc tcgtcaacgc cggactggtc
    19801 cgaggtctca aggtcccctc gatcatcgcc accctggcca cgttgagcat cctggacggg
    19861 atctccttga ccctgcggcc gacggcgcag ggggtcatca gccccgacct ggtgaccgtc
    19921 ctcaagacga gcatcggacc gatccccgtg gcgttcatcg tcgtggtcgc cgcggccgcg
    19981 ctggccgatc tctggctgca cgcatcggga tcgggactcg cgctgcgggc cgtcggtttc
    20041 gacgagcggt cggcgaagcg cggtggcgtc ggcaccaatt ggctccgggt ccgagccctc
    20101 ctgctgtcag cggtgctggc ggcggtggcg tccgtcttcg tcatggcccg atccccgatc
    20161 ggcaacgcac aggtcggatc gaccttcgcc ctgaacagca tcaccgccgc cgtgctggga
    20221 ggcgcgtccc tcgccggtgg ccggaccacg ttcatcggcg ggaccgtggc ggcgatcctc
    20281 ctcgcgctca tcctcaccgt cttgccgtac ctcggtctgt cacccaacga cggaccgatg
    20341 atcatcggcg tcttggtgct gatcgggatc gtcctgttcc agctcggcga catcaaggag
    20401 ctggcgaaga ggaactacaa gcgagccagg cgcttgatga tcggcagtcg cccgccgaag
    20461 gctgccagtc ttcccgcgtt ctatcccgcc gggaccgact tcagcgtggc gccgtccggg
    20521 aggaccttga tccggggtgg gaccgtcctc agcctcgacc cggcgctggg cgacttcgcc
    20581 accggggacg tgctgatcga aggagaaacg atcctcgccg tcggtcccga tctgaccgac
    20641 ggcgaggtcg agacgatcga cgcgtcgggg atgatcgtca tgcccggatt cgtcgactcc
    20701 caccgtcaca tctgggaggg gctcctccgg aacatcggga ccgacgtccc tctggaggga
    20761 cggtccagct acatctcgtt cgtcctgcac aagttggcgc ccgcgttccg tccagaggac
    20821 gcctacatcg gcgacctcgt ctcggcgctg ggcgcgatcg atgccggcgt cacgacgctg
    20881 ctcgactggt cgcacatcca gggctcgccc gcgcacaccg atgcggtgat ccaggcactc
    20941 aaggactccg ggctgcgcgc ggtcttcgcc tacgggttcc cctggtgggg caagtgggag
    21001 gagcgacagc ccagctggtt cgtgcgcgcg gcgacggagc acttctccac gaaggatcag
    21061 atgctgacgc tggccctggc ggcacccggg cccgagttca ccgacttcga ggtgtcgcgg
    21121 gatcactgga agctcgcccg cgaggcggac gcgcggatca cgacccatgt cggcgtcggg
    21181 agctacgggc aggacgccaa ggtccaggag atgggagagg cgggactgct cggcccggac
    21241 acgacgtaca tccactgcac gacgctgaac gacaccgaga tccagatgat cgtggacacc
    21301 ggcggaacgg tgtccctggc gtcgccggtc gagatgatga tgggccacgg gatgcccccg
    21361 atccagaagt tcctcgaccg gggactccgc ccgagcctga gcgtggacgt cgagacgaac
    21421 gtgccggccg acatgttcaa ccagatgcgt tcggtactgg gcctgcaacg ggccgtcgcc
    21481 acagcagccg gcaagtcgcc gctgtccgcc gccgaggtgc tcgcctgtgc cacgatcgag
    21541 ggcgcccgcg cgaacggcct cgacggggtc gccgggtcgc tcacgcccgg caagaaggcg
    21601 gacgtcgtcc tgctccgcac ggaccggatg aacgtgaccc cgctcaacga tccggcaacc
    21661 gcggtcgtca ccgggatgga caccggcaac gtcgacacgg tcctgatcgg gggacgggtg
    21721 atgaagcgtc acggcgagct gctccacgtc gactggccgg ccgtgcggcg gatggccgaa
    21781 gagtcgaggg acttcgtcgt gtcgagatcg ggcttcaaac tcccggggat ctgatgagga
    21841 gacggtcgcg cgaaagatgg gatcgagcga tggacggtac gagcgcgctc cggcgcttcg
    21901 cgttcgatcg cgacgtccag cctggatcga gttcacctag catgcagccc gcgacgccgg
    21961 atgaggctct acgacggccg ggccgccccg agcatcggag gagatgaggc acatgccgac
    22021 gacggacgga ttcccggtcg cgacgacccc cgcgcccggc atcgctggag tcgactggga
    22081 ggttcgcgtc gacttcgagc gactccggac ctatcgactc gatcgcgcca aggacgcgtt
    22141 ggaggcatcg gagctcggtg ccctgctcct gttcgagacg agcaacatcc gctacgtcac
    22201 cgggacccag atcggctact gggcgttcaa caagggggag cgctacgccc tcctcacgcg
    22261 caccgggagg ccccggatct tcgacttcgg ctcggcggcg aaggcccatc ggctccagct
    22321 cccccacctg tacgacgaga cgaactcggt cggcgggaac acggggctcc agggtgcgat
    22381 ccatccgcgc gtgggtctcc aggcgcgcgc cgtgcaggag atccgttcga tcatgaccga
    22441 ggacggcgtc ggcgatatgc cgctcggggt cgacgtcgcc gagacgtcca tcttcctcga
    22501 gctgatgcag gcggggatcg aggtccgcga cgggcaacag gtgatggccg acgcgcggga
    22561 gatcaagagc cccgacgagc tcatgctcct gacccaggcg tgcgcgatgg tggacggcgt
    22621 ctaccaggac atcttcgagg cgctgaagcc gggtgtccgc gagagcgaca tcgttgggct
    22681 cgcgcacgcc cgtctcttcg agatgggttc ggagttcgtc gaggcgatca actcgatcgc
    22741 cggggagcgg tgcagcccgc atccgcacgt gttctcggac cggctgatcc ggccgggtga
    22801 ccaggcatac ttcgacatca tccatgtctt caacggctac cggacctgct attaccgcac
    22861 gttcgtcgtc ggccgggcga cgcaggcgca gcgagacgcg ttcgcgcgct cgcgggaatg
    22921 gatggatgcc gcgatcgatc tcgtgaaacc cggcgtcgcg acggaccaga tcgcggccgt
    22981 ctggccgaag gccgaggatt tcgggttcgc cgacgagatg gacgcgttcg ggttgcagtt
    23041 cggccacggg gtgggcgtga gcctgcacga gcggccgatc atcacgaggc tgaactcgtt
    23101 cgtggatccc ctcgagatca aggaagggat gctcttcgcc ctcgagacgt attgcccggc
    23161 cgcggacggc cgatcggccg cgcgcatcga ggaggaggtc atcgtcaccg ccgacggctg
    23221 ccgggtgatc acgttgttcc cgtgcgacga actcatggtc gcgaacgagt actgagggcc
    23281 aaggtgtgca gaagggaacg ttgatgccga acacggacac ggccgagggg accgagatcg
    23341 cggatctcga ggccaggctc tcgatctacc ggatgatgca ggagggccgc ctcttcgagg
    23401 agcgagcgag ggacctgttc ctgcagggat tggtcaaggg cacctcccac ctcgggatcg
    23461 gccaggaagc gatcgccgcc ggtttcgcgg cggcgctcga gccggatgac ctcgtgttct
    23521 gcacgtaccg cgggcacgtc cacacgttgc tgcgaggagc accgatggcc gcgctgatgg
    23581 ctgagctcct ggggcgcgcc acgggcatct gcggtggcaa gggtggctcg atgcacctca
    23641 cggacgtctc gcacggcgcg atgggctcct acgcgatcgt cggggcccac atgccgatcg
    23701 ccgccggcgc cgcgtgggct gccagggtcc gcggaacggg ccaggtgacc gtgtgcttct
    23761 tcggtgacgg gacgacgaac atcgggtcgt tccacgaggc g
//
LOCUS       DASUYD010000135         3771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_954956, whole genome shotgun sequence.
ACCESSION   DASUYD010000135 DASUYD010000000
VERSION     DASUYD010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954956"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..495
                     /locus_tag="VF195_07390"
     CDS             67..495
                     /locus_tag="VF195_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580678.1"
                     /translation="MSEPVFFISHFAIKEGALEDLKGRTGEVMQRLREEKPRTVLFLS
                     YLDEEGGRISFLHAFPDADALDEHFAGADERAAAAYEFIEPRGWELYGQTSEQVMEGM
                     RATAERFGVPLVVEPRFNGGFLRLAPDSPPGTFASTRTRS"
     gene            535..999
                     /locus_tag="VF195_07395"
     CDS             535..999
                     /locus_tag="VF195_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX6580679.1"
                     /translation="MVNGKHGDSPLKTTTVETAERGRSPLGAFVRYLMDGPHGNMIHS
                     TVPPGMVGRACRFRTIDEYWFVLSGEGEIWRRATDDRESITRLIPGVSIDIPLGTTFQ
                     YRCTGDVPLVFTCTALPSWPGDDEAVITDGPWVPSPDLELGASRSDQPPQHA"
     gene            1140..1736
                     /locus_tag="VF195_07400"
     CDS             1140..1736
                     /locus_tag="VF195_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009357021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580680.1"
                     /translation="MTMVVAAITISLDGYVTGPNDGPGRGLGDGGERLHDWVFGGPWS
                     YEAEPSGEATGADKEYLDGAMARAGAVVVGRGMYEAAEAWGGQNPFGVPLFVLTHRPE
                     DAPSEGGFTFVDGLDEAIVLARQAAGAKDISVGGGADVIRQALRTGYVDELAISIAPL
                     ILAGGKRLFDGFEQTLNLEHAALLQSPFATHITYRLVR"
     gene            1796..2164
                     /locus_tag="VF195_07405"
     CDS             1796..2164
                     /locus_tag="VF195_07405"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015101705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6580681.1"
                     /translation="MECWAGWIVVGVLALATGATIAGCESRYDRSIAGTIASFMIDDR
                     TEVTYWIDRALWGRGIASAALQSFLPMTAERPLFARAASDNAASLRVLEKAGFRRIGV
                     NRDFARGRGEEIEETILRLD"
     gene            complement(2294..2857)
                     /locus_tag="VF195_07410"
     CDS             complement(2294..2857)
                     /locus_tag="VF195_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580682.1"
                     /translation="MGNVVIDMSMSLDGYIAAPNDTPEQGLGEDGMRLHNWMFDDPTV
                     FQRVYGDLTESTGAVIMGRRTYDNSIEAWGGKGPLGEVPCFVVTHRPIEGADPLFTFV
                     TDGIESALAKAQGAAGDKRIGLMGANLDQQFLAAGLVNEIRIYLLDVLLGGGRRLFDQ
                     LPKRVELERTALSQTGGVTHLEYRVAG"
     gene            complement(2915..3508)
                     /locus_tag="VF195_07415"
     CDS             complement(2915..3508)
                     /locus_tag="VF195_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580683.1"
                     /translation="MSDAIVQATPGRVPALAELLGRAFTDDPMLVWPLGADRTELVTD
                     FFRAFDERIAVHGWLWEAGDGLGAAAWIPPGSDQIMMDIDRSMRSTLANAGARHEEMW
                     EWIARHFPDEPFWYLDHIAVDAAHRGTGLGGALIRLGLDFADRDGVPAFLETGRPGNL
                     GYYERRGFRTISDDDAPGGGPHIWFMRYDPRPSVPGS"
     gene            3580..>3771
                     /locus_tag="VF195_07420"
     CDS             3580..>3771
                     /locus_tag="VF195_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580684.1"
                     /translation="MLEQETIREREGRWKPRPTLSGSDYTSPEVWEEERERIWFGDWV
                     CIGRAEEVANPGDYLVRDLV"
BASE COUNT          610 a         1238 c         1286 g          637 t
ORIGIN      
        1 gcgggacact cggtcgcttt gtccgcgagc cggttcgaac ctacgatccc ccgaggaggt
       61 ggtcggatgt ccgagcctgt gttcttcatc agtcacttcg cgatcaagga gggcgcgctc
      121 gaggacctga aggggcgcac cggggaggtg atgcagcgcc tgcgcgagga gaagcccagg
      181 acggtcctct tcctctcata cctggacgag gagggcggcc ggatctcgtt cctacacgca
      241 tttccggatg ccgacgcgtt ggacgagcac ttcgcgggcg cggacgagcg ggccgcagcc
      301 gcgtacgagt tcatcgaacc ccgagggtgg gagttgtacg gacagacgag cgagcaggtg
      361 atggagggta tgcgagcaac ggcggagagg ttcggcgtcc ccttggtggt agagccgcgg
      421 ttcaacggcg gcttcctgcg cctggcccct gatagccctc caggaacatt cgccagcact
      481 cgcacacgca gctgaggtct acctcgaggc ccagggtgtg aggttgaatg tggcatggtg
      541 aatgggaaac acggcgacag tccgttgaag accaccaccg tcgaaaccgc ggagcgcggc
      601 cgctcgccct tgggagcttt tgttcgctac ctgatggacg ggccgcacgg caacatgatc
      661 cactcgaccg tgccgccggg gatggtgggc cgcgcctgcc gcttccggac catcgacgaa
      721 tattggttcg tcctctcggg cgagggagaa atctggcgca gggcaacgga cgaccgtgag
      781 agcatcaccc ggctcatccc cggtgtgagc atcgatatcc cgctcggcac taccttccag
      841 tatcgctgca ccggggatgt gccgcttgtc ttcacctgca cggccctgcc gtcctggccg
      901 ggcgacgacg aggcggtgat cacggacggg ccgtgggtac caagtccgga cctggagctc
      961 ggggccagcc gctcggatca gccccctcag cacgcctgac cttccgccaa gtatgggtcc
     1021 agggacattc gcgaacactc ggacctgcga gcttctccct ccctgtgtgt tcgaccctag
     1081 gatcgtcgag cggctgggac cgtatcggtc aggatggtcg acgagcagga gggaacgcca
     1141 tgacgatggt ggtagcggca atcacgatct cgctggacgg ttacgtcaca ggtccgaacg
     1201 acgggccggg gcgcggcctg ggcgatggcg gcgagcgatt gcacgactgg gtcttcggcg
     1261 gcccgtggag ctacgaggcg gagccgagcg gcgaggcgac gggcgcggac aaggagtacc
     1321 tcgacggagc gatggctcgc gccggtgccg tcgttgtcgg acgggggatg tatgaggccg
     1381 cggaggcgtg gggcggtcag aacccgttcg gcgtgccgct cttcgtcctg acccaccggc
     1441 ccgaggacgc tccgagcgag ggcggcttca cattcgtgga cggcctggac gaggcgatcg
     1501 tcctggcgcg ccaggcggcg ggtgccaagg acatatccgt cggagggggc gcggacgtga
     1561 tccgtcaggc acttcgcacc ggatacgtgg acgagctggc gatctcgatc gcgcccctca
     1621 tcctcgccgg tggcaagcgc ctgttcgacg gcttcgagca gacattgaac ctggagcacg
     1681 cggccctgct ccagtcaccc ttcgccacgc atatcaccta tcgcctcgtc cgctgaccgg
     1741 cggcgttcga gttcggtccg tgactgtcct gcggttctga atgaaggttg gagagatgga
     1801 gtgttgggcg ggctggatcg ttgtcggagt cctggcgctc gccaccggag ctaccatcgc
     1861 cggatgcgag tctcgctacg accgctcgat cgccgggacg atcgcctcct tcatgatcga
     1921 cgaccggacc gaggtgacct attggatcga tcgggcgctc tggggcagag gtatcgccag
     1981 tgccgcgctc cagagcttcc tccccatgac tgctgagcgc ccgctgttcg cccgtgcagc
     2041 ctcggacaat gccgcgtcgc tgcgggttct tgagaaggcc gggttccgac gcatcggcgt
     2101 caatcgcgac ttcgcccgcg gtcgcggaga ggagatcgag gagacgatcc tgcgtctcga
     2161 ctgagcagcc cggctcgaag cggttcccga tcgcgacggt gttcgactac tcgttcgacg
     2221 gtcttctcgg ccgacgaggg cacccaattc tggaagttcg tctcgaccta cccgagatcc
     2281 gtgcttggaa ccgctagccg gcaacgcggt actcgagatg cgtcaccccg ccggtctggc
     2341 tgagcgcggt ccgttccagc tcgacccgct tggggagctg gtcgaacaac cgacggccgc
     2401 cgccgagcag gacatcgagc aggtagatcc tgatctcgtt cacgagcccc gctgccagga
     2461 actgctggtc gaggttcgcg cccatgagcc cgatccgctt gtcgccggcg gccccctggg
     2521 ccttcgcgag ggcactctcg atcccgtcgg tgacgaacgt gaacagcggg tcggcgccct
     2581 cgatgggtct gtgcgtgacc acgaagcagg ggacctcgcc cagcgggccc ttgccacccc
     2641 acgcttcgat cgagttgtcg tacgtccgtc ggcccatgat cacggcgccg gtgctctcgg
     2701 tcaggtcacc gtagacgcgc tggaagaccg tcggatcgtc gaacatccag ttgtgcagcc
     2761 gcatgccgtc ctcgccgaga ccctgctccg gtgtgtcgtt cggcgctgcg atgtagccgt
     2821 ccaatgacat gctcatgtcg atcaccacgt tgcccatgtc gcgtcctccc gtcgtcgatg
     2881 acaccagccg cggtgacgaa cctaccgtgg gagctcagga ccctggcacg ctcggccggg
     2941 ggtcgtagcg catgaaccag atgtgcgggc cgccgcccgg cgcgtcgtcg tccgagatcg
     3001 tgcggaagcc tcggcgctcg tagtacccca ggttgcccgg ccgtcccgtc tcgaggaacg
     3061 ccggcacgcc gtctcgatcg gcgaagtcca gtccgaggcg gatcagcgct cctcccaggc
     3121 ccgtgcctcg atgcgcggcg tccaccgcga tgtgatcgag gtaccagaac ggctcgtccg
     3181 ggaagtgcct cgcgatccac tcccacatct cctcgtggcg ggcacccgcg ttcgcgagcg
     3241 tcgagcgcat cgaccggtcg atgtccatca tgatctggtc gctcccggga gggatccatg
     3301 cggcggctcc gaggccgtcg ccggcttccc acagccatcc gtgaaccgcg atccgctcgt
     3361 cgaacgcgcg gaagaagtcc gtgaccagtt cggtcctgtc ggcccccaac ggccacacga
     3421 gcatcgggtc atccgtgaac gcgcgaccga ggagctcagc aagcgccggc acccgcccgg
     3481 gcgtcgcctg gacgatcgca tcggacatcg gcggagccta tccgccgacg aggtacgtcc
     3541 gtcgcctatc ctttcccttc cacgtacgga ggacgcagga tgctcgagca ggagacgatc
     3601 agggagcgtg agggccgatg gaagccgcgg cccacgctga gcggcagcga ctacacgagc
     3661 cccgaggtct gggaagagga acgcgagcgg atctggttcg gcgactgggt ctgcatcggc
     3721 cgcgccgagg aggtggcgaa ccccggcgac tacctggtca gggacctcgt c
//
LOCUS       DASUYD010000136         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_733022, whole genome shotgun sequence.
ACCESSION   DASUYD010000136 DASUYD010000000
VERSION     DASUYD010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733022"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF195_07425"
     CDS             complement(<1..414)
                     /locus_tag="VF195_07425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6580685.1"
                     /translation="MSEPAHLLELHDVAKHFGGLYALSGLTLHVDEGEIVSVIGPNGA
                     GKSTMFNVVTGLYEPDDGQIRFRGDNVVGLAPNQVTKLGIARTFQTVHLFPNMTILEN
                     AMVGQHCRTRSGVVGSVLRLPGTRREEERIRARARE"
     gene            complement(411..2168)
                     /locus_tag="VF195_07430"
     CDS             complement(411..2168)
                     /locus_tag="VF195_07430"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine/isoleucine/valine transporter permease
                     subunit"
                     /protein_id="HEX6580686.1"
                     /translation="MIGGATAIYVALVGLYTKFADLALVGEQITLGRLLIVAPAIGAA
                     YVVTRTKIVAGERRPVPFGGGLSVGAITGLVGGTLFAVAIAFADWFGVERIREVFVAV
                     TEPLIDFLTFGDGVPGGLPILIGASTVGGVIGAFARTAPASVRKPASVGVLAVVLFGL
                     LQRIVPIVFVELGFEADWLYRPASGLTYLGATVVFAIAVIADVLWNRDGGRTRERVRK
                     SVEEAPAAKLGLLVAIMAILAIVPLLLGTVISGVLGQVMIYVLLGLGLNIVVGYAGLL
                     DLGYVAFFAFGAYTTGLLTGAFLNTTTGAAEPALSADLNFYVAVWIVALLAAGVGLLI
                     GAPVLRLRGDYLAIVTLGLGEIVSVITASKWAQPLIGGPQGMRGVTKAELFGFNFQDN
                     SQHFYYLALAFVLLAAFVSWRLSTSRIGRAWNAMREDEQVADAMGISTTRFKLLAFAL
                     GGAIGSLGGALFAVSIGSLTPASFEILVSITALAVVILGGLGSIPGVVVGALVLIGLP
                     GLLREFEEYRSLIYGGALVAIMILRPQGLIPNVRRSRELEEEEVTQDAWAKDASGERE
                     AAAAVLPGGGLGVEREDLG"
     gene            complement(2207..3307)
                     /locus_tag="VF195_07435"
     CDS             complement(2207..3307)
                     /locus_tag="VF195_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6580687.1"
                     /translation="MATATDEQVRGARLGLLERFPWRRIILIILSIVIVAVVAIGAYK
                     TLTLPADERYTATTWKNFIVLGLAQGAIIGLIALGYTLVYGILRMINFAHGEVFMFGA
                     FGAFFFSDAWEESGFLDSNPILSLGLLLVIAMLTSMGVAVLLERIAYRPLRNAPRLVP
                     LITAIGASLFLANTARGFFGAQSRGFGVPAALEGQWTILGVSVDRIQVVVLLTVIVAV
                     TALSVFVSRTTTGKSMRAVAADSEIASLMGIRVDRVIVTTFAIGGLLAGIAAVLFSIT
                     FESVSFTMGFRPGIAAFTAAVLGGIGSISGAALGGVLLGLLQSVGPALILTGYGIPSS
                     FQLKDAFTFLVLVLVLIFRPGGLLGSGEAEKV"
     gene            complement(3387..4727)
                     /locus_tag="VF195_07440"
     CDS             complement(3387..4727)
                     /locus_tag="VF195_07440"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6580688.1"
                     /translation="MKNRKYLSMLALVGALMLVAAACGDDDGGGETSATGGSATGAAV
                     DCEADEFGCVEIAAGDPIQLASLLSISGDTAFLGTDSNNGVTLAIDHLDDAFDATPGQ
                     LLGHDVTLQQEDDGCSAEGGTLGAEALAANPDIVAVIGTSCSSAALGVADTILGSKGM
                     LLFSPSNTNPGLTSEEAHQPFYARTAHNDKIQGAIVADFVFTELGIDTAATINDESPY
                     ADGLAAAFRDNFEALGGTITGIEQVSSSDTDLGPVFTNIASGDPGVVYGPNFNPVCAL
                     SYTQGADILAKGTVQIGSDGCLESSFVETVGAPISGADYFASSPDVTAYQGGAFYKDE
                     YLPAYTDQFGKPTSVFNAHAYDAANVLFDAISAVAVDDGSGNLTISRTALKDAVLATS
                     GYEGLTGTITCTPLGDCATDVTIGVFEYDHWPVEGGVDPTDPVFSETKTLADVI"
BASE COUNT          843 a         1697 c         1637 g          718 t
ORIGIN      
        1 ctccctcgcc ctggctcgga tccgctcctc ctcgcgccgg gtcccgggca gccggagcac
       61 cgagccgacc acgccggacc tggtccggca gtgctgaccg accatcgcgt tctccaggat
      121 cgtcatgttc gggaacaggt gcacggtctg gaacgtccgg gcgatgccga gcttcgtcac
      181 ctggttcggg gcgaggccga cgacgttgtc cccacggaag cggatctggc cgtcgtcggg
      241 ttcgtacaga ccggtgacga cgttgaacat ggtgctcttg cccgccccgt tcgggccgat
      301 caccgagacg atctctccct cgtcgacgtg gagggtcagg cccgagagcg cgtagaggcc
      361 cccgaagtgc ttggccacgt cgtggagctc gagcaggtgc gccggttcgc tcatccgagg
      421 tcctcgcgtt cgacgccgag accgccaccg ggcagcaccg cggcggcagc ctctcgttcc
      481 ccgctcgcgt ccttggccca ggcatcctgc gtaacctctt cttcctccag ctcgcggctc
      541 cgacggacgt tcgggatcag gccctgcggt cgcaggatca tgatggccac gagcgcgcct
      601 ccgtagatca gggaccggta ttcctcgaac tctcgcagga gacctggcag gccgatcagc
      661 acgagcgcgc cgaccacgac acccgggatg ctgccgaggc cgccgaggat caccaccgcg
      721 agcgccgtga tggacacgag gatctcgaag ctcgcgggcg tgagcgagcc gatgctgacg
      781 gcgaacagcg ctcccccgag cgagccgatc gcgccgccca acgcgaaagc gagcagcttg
      841 aacctcgtcg tgctgatccc catggcatcg gcgacctgct cgtcttcccg catcgcgttc
      901 cacgctcgac cgatccgcga cgtcgataac cgccacgaga cgaaggccgc gagtaagacg
      961 aacgcaagcg cgaggtagta gaagtgctgc gagttgtcct ggaagttgaa cccgaagagt
     1021 tcggccttgg tgacgccgcg catcccctgc gggccaccga tgagcggctg agcccacttc
     1081 gacgccgtga tgacgctcac gatctcaccg agtcccagcg tgacgatcgc caggtagtcc
     1141 ccccgaagcc gaaggaccgg tgcgccgatc agtaggccga cgccagccgc gagtagggcg
     1201 acgatccaca ccgcgacgta gaagttcagg tcggccgaga gcgcgggctc ggcggctccg
     1261 gtggtcgtgt tgaggaacgc accggtgagc agtccggtcg tgtaggcgcc gaacgcgaag
     1321 aaggcgacgt agccgaggtc gagcagcccg gcgtagccca cgacgatgtt cagacccagg
     1381 ccgagcagga cgtagatcat cacctggccg aggacccccg agatgacggt cccgagcagc
     1441 agcgggacga tcgcgaggat ggccatgatc gcgaccagca gcccgagctt ggcggccggt
     1501 gcttcctcga cggacttccg gacgcgttcg cgcgtacggc cgccgtctcg gttccacagg
     1561 acgtccgcga tcacagcgat cgcgaagacc accgtcgcgc cgaggtaggt cagtccgctg
     1621 gccggacggt acagccagtc cgcctcgaac cccagctcga cgaagacgat cgggacgatc
     1681 ctttggagga ggccgaagag cacgacggcg agcaccccca ccgacgcggg tttgcggacc
     1741 gaggccggcg cggtacgagc gaacgcgccg atcacgccgc ccacagtgga cgctccgatg
     1801 aggatcggca agccgccggg gaccccatcg ccgaacgtga ggaagtcgat caacggctcg
     1861 gtcaccgcga cgaagacctc gcggatccgc tcgacgccga accagtccgc gaaggcgatc
     1921 gcgacggcga agagggttcc gcccacgagc cccgtgatgg ccccgacgga cagcccgccc
     1981 ccgaacggga ccggccgtcg ctcccccgcg acgatcttcg tgcgcgtcac cacgtacgcg
     2041 gcgccgatcg cgggcgcgac gatcaagagc cgaccgagcg tgatctgctc ccccaccagg
     2101 gcgaggtcgg caaacttcgt gtagaggccg accagcgcca cgtagatcgc cgttgctcct
     2161 ccgatcatcc ccccgctgat gaccgaccgt gaagggacgc cgcccatcac accttctccg
     2221 cctcgcccga tccgaggagc ccgcccgggc ggaagatcag caccagcacc aggaccagga
     2281 acgtgaacgc gtccttgagc tggaacgatg aggggatgcc gtacccggtg aggatcagcg
     2341 ccgggccgac cgactggagg agtcccagca ggaccccgcc gagcgcggcg cccgagatgc
     2401 tgccgatccc tccgaggacc gccgcggtga acgccgcgat ccctggacgg aaccccatcg
     2461 tgaagctcac cgactcgaac gtgatcgaga acagcactgc ggcgatgccc gccagcaatc
     2521 ccccgatcgc gaaggtcgtg acgatcacgc ggtccacgcg gatccccatc aggctcgcga
     2581 tctcgctgtc ggcggccacg gcacgcatcg acttcccggt cgtggtccgc gagacgaaga
     2641 ccgacagggc cgtgacggcg acgatcacgg tgagcagcac gacgacctgg atccggtcga
     2701 ccgagacgcc gaggatcgtc cactggccct cgagtgccgc cgggacgccg aacccgcggc
     2761 tctgcgctcc gaagaagccg cgggcggtgt tcgccaagaa cagcgaggcg ccgatcgccg
     2821 tgatgagcgg cacgagccga ggcgcgttgc ggagggggcg atacgcgatc cgttccagca
     2881 agacggcgac gcccatcgac gtcagcatcg cgatcacgag caggagcccc aacgacagga
     2941 tcgggttgct gtccaagaac ccgctctcct cccacgcgtc ggagaagaag aaggcgccga
     3001 acgcgccgaa catgaacacc tcgccgtggg cgaagttgat catccgcagg atcccgtaca
     3061 cgagggtgta cccgagggcg atgagcccga tgatggcgcc ttgcgcgaga ccgaggacga
     3121 tgaagttctt ccacgtcgtc gcggtatagc gttcgtcggc cggaagggtc agcgtcttgt
     3181 acgcgccgat cgcgacgacg gccacgatca cgatcgacag gatgatcagg atgatcctgc
     3241 gccacgggaa gcgttccagg agtccgagac gcgcgccgcg gacctgttcg tcggtggcgg
     3301 tggccatcgc gcgagcctag cccatccccc gaggcagcgc tgcgggtggg cccttggccc
     3361 acccgcagct tgcgataccg atcgagctag atgacgtcgg ccagcgtctt ggtctccgag
     3421 aagaccgggt cggtcgggtc tactccaccc tcgaccggcc agtggtcgta ctcgaagacg
     3481 ccgatcgtga cgtcggtcgc gcagtccccg agcggggtgc acgtgatcgt gcccgtcagg
     3541 ccctcgtagc cgctcgtcgc caggacggcg tccttgagcg cggtccggga gatcgtcagg
     3601 ttcccgctgc cgtcgtccac cgccacggcc gagatcgcgt cgaacaggac gttcgccgcg
     3661 tcgtaggcgt gagcgttgaa caccgacgtg ggcttgccga actgatccgt gtaggccggg
     3721 aggtactcgt ccttgtagaa ggcacctccc tggtacgccg tcacgtccgg cgacgatgcg
     3781 aagtagtccg cgcccgagat cggggcgccg accgtctcca cgaacgagct ctccagacaa
     3841 ccgtcggagc cgatctggac cgtccccttg gccaggatgt ccgcgccctg cgtgtagctc
     3901 agggcgcaca cggggttgaa gttcggcccg tagacgaccc cggggtcacc cgaggcgatg
     3961 ttggtgaaca cgggaccgag atccgtgtcg gagctgctca cctgctcgat gccggtgatc
     4021 gtgccgccga gcgcctcgaa gttgtcgcgg aatgcggcag ccagaccgtc ggcgtagggg
     4081 ctctcgtcgt tgatcgtcgc ggccgtgtcg atgccgagct ccgtgaagac gaagtcggcc
     4141 acgatcgcgc cctggatctt gtcgttgtgc gcggtccgag cgtagaaggg ctggtgcgcc
     4201 tcctccgacg tgagtcccgg gttcgtgttc gacggcgaga acaggagcat ccccttgctg
     4261 ccgaggatcg tgtcggcgac gccgagcgcg gcgctcgagc acgaggtccc gatcaccgcg
     4321 acgatgtcgg ggttggccgc gagcgcctcc gcgccgagcg tcccaccctc cgccgagcac
     4381 ccgtcgtcct cctgttggag ggtgacgtcg tggccgagga gctgtcccgg tgtcgcgtcg
     4441 aacgcgtcgt cgaggtggtc gatcgcgagc gtgacgccgt tgttgctgtc cgtccccagg
     4501 aacgcggtgt cccccgagat cgagagcaac gacgctagct ggatcgggtc acccgcggcg
     4561 atctccacgc agccgaactc gtcggcctcg cagtcgacgg ccgcgcccgt ggcgctcccg
     4621 cccgtggcgc tggtctcgcc gcccccgtcg tcgtcgccgc aggcggcggc gacgagcatg
     4681 agcgctccga cgagcgccaa catcgaaaga tacttgcggt tcttcatcca acccccctcc
     4741 gtagctgtcc tacgatcgtt gcggtgcgcg aacatgcaga gtggaacggt ggttcgcccc
     4801 ctttctcacc tccgtcgggg tcgtacctat cacatccggc acgccggggg ccactccggg
     4861 gcacccgacg cggttcctca ggatgtacgg aacgc
//
LOCUS       DASUYD010000137         8223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_733157, whole genome shotgun sequence.
ACCESSION   DASUYD010000137 DASUYD010000000
VERSION     DASUYD010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_733157"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1234
                     /gene="ugpC"
                     /locus_tag="VF195_07445"
     CDS             2..1234
                     /gene="ugpC"
                     /locus_tag="VF195_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017602129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX6580689.1"
                     /translation="MDHVSKVYGAEGPSAVSDLNLEIEDGEFIVLVGPSGCGKTTALR
                     MVAGLESITDGTISIDERVVNTVPPKERDIAMVFQNYALYPHMTVYDNMAFGLKLRKL
                     SKEEIDRRVREAAKILGLDDFLERKPKALSGGQRQRVAMGRAIVREPKAFLMDEPLSN
                     LDAKLRVQMRSEIARIQHDLNVTTLYVTHDQVEAMTMGDRVAVIRKGVLQQVDSPQFL
                     YDHPENLFVAGFIGSPAMNMVEADLGRENGTWAVTFGGFSLEIPDEVFAARPALRAFE
                     GKPVVIGIRPEDMEDASLISDAPRNRRIRSAVILREALGADVLVHFGVRARPVITEDT
                     KELAHDVGAEVLDAVERAGDQGEWIFLARLSPRTQAKQGKEIELVVDTSRLHFFDPET
                     GRGIYDGEPATTETRTTT"
     gene            1272..2582
                     /locus_tag="VF195_07450"
     CDS             1272..2582
                     /locus_tag="VF195_07450"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580690.1"
                     /translation="MRRLRMLAIPAVIALIAAACTTDEDGGDGNGGGQEENTGTVNVL
                     NALEPEENVVLQSISDRLIAPDADYEVEFEQVDDVAQQIQIRAEGGTLDVVLTPQPGA
                     VQEQAANGNAVSLEDLGFDIADLESTFGEYFLSLGEFEGEHYGIPTNINLKSMVWYAK
                     DDFDAAGYTIPETWDDLIALSDEMVANGDTPWCVGFEAGGDTGWPATDWMEDIVLGSA
                     GTETYTQWINHDIPFTDPAVVHAGELFGDMMFTEGYVLGGAENTPATAFADAPAPLFE
                     DPPGCFMHRQASFITAFFGDAESGVDYDWFPMPGESQRPTLFAGELATVFSNRPEVVE
                     FLEAFMGEEVQCAMGSEPGSSRISPNVNVGPDCYTDPILADASEVLTAALAGEGAPGF
                     DASDQMPPQVGSGSFWTEMMGYMEEGPDSLQTHLENIQASWPAE"
     gene            2670..3806
                     /locus_tag="VF195_07455"
     CDS             2670..3806
                     /locus_tag="VF195_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014456508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6580691.1"
                     /translation="MAPDDAAPATIDERPTGPQAVLVSILRLIGAFAIPIVGFFILWA
                     TFDFLREADANRLIVVGVAILVGVGGVFFLYWGMNRVVDFLPVRYREGVRPYIFVGPA
                     LVILAVFLIYPVLNTILVSFKDAQGESFVGLDNFRFVFTDESMLRAIRNTFGWIVIVP
                     LLGVTIGLAFATLADRLRRGEAIAKSMIFLPMAISFAGASITWRLIYNFRPQGFGSNI
                     GLLNGIMLGLGQEPVRWLDIEPWNNLMLMVIMVWMQTGFAMVVLSAAIKSIPDEILEA
                     ARIDGASELQVFRRIVVPSILPTIAVVTTYMVINAMKVFDIVFVMGSPETRGTEVIAE
                     RMIDWFFINNNDGRGAAIAVILFIAIVPVMIVNIRRFREEEAIR"
     gene            3899..4744
                     /locus_tag="VF195_07460"
     CDS             3899..4744
                     /locus_tag="VF195_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006516104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6580692.1"
                     /translation="MFAVVILWIIPTLGVLLTSFRPEALVDSTGWWTALAHPFRTTEW
                     TLENYRLALDAGGLGNAFLNSLAVAVPATVIPIAIAAFAAYAFSWMEFRGRYVMFVGV
                     VALLVVPLQMALIPILRLYNNGGVVGRYIIFPDLDLVGTFLGVWLAHTGFGLPLAVYL
                     LRNYIGSLPSSIIESAKMDGADHFTIFWRLIIPLSVPALAAFAIFQFLWVWNDLLVAY
                     VFLGGTQQNRVLTIALANLVGGRGENWHLLTAAAFISMALPLVVFFALQKYFVRGLTA
                     GAVKG"
     gene            4750..5325
                     /locus_tag="VF195_07465"
     CDS             4750..5325
                     /locus_tag="VF195_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580693.1"
                     /translation="MSRPRASSAPSFEPLPPRKKWRAITIATLALLPAYLSIVVGLVA
                     VASDRADAPPAGPAIAFGLGLLPFVFVALAFLSSHPRAPGAAAKAMALSLLVGIPVAA
                     LVPDAVTGLVAGIGAGGAAALRPEPDGSWRARALGVAAVSAYAAVMVRIVPDVTVLLA
                     PTLPFTCLGVADHLREQRRERARGREVAGRP"
     gene            5322..7772
                     /locus_tag="VF195_07470"
     CDS             5322..7772
                     /locus_tag="VF195_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6580694.1"
                     /translation="MTRFGRFDDAAREYVITRPDTPLPWINYLGSEEFFSIISNTAGG
                     YSFFRDARLRRLTRYRYNAAPADTGARFLYLRDAATGAFWSPSWQPVRTELEDYRCRH
                     GLGYTVIGSRTSGIETESLYLVPLGSPLEIWRTAVTNRRADTARLSLFGAVEFCLWDA
                     WDDQTNYQRNLSTGEVRVLDGVIYHTTEYRERRNHFAYFACSEEVSGFDTQREEFLGP
                     YRGWERPAVVEVGRSRNSIANGWAPIASHQVELELAPKETREVVFLLGYGENPPDAKF
                     NPPGSTRLDRRPVLPVIERYGQRGEIDVALERLRDRWAELLDVVRVTTPNEHVDRMVN
                     VWNPYQCMATFNLSRSASSFDTGIGRGIGFRDSSQDVLGSVHMVPERARGRILDLAAT
                     QLPTGGAYHQYQPLTKRGNDTIGSGFNDDPLWLILAVASYVKETGDRQILDEPAPYDN
                     KPGTETPLYEHLERALAFTTDRLGPHGLPLIGRADWNDCLNLNAFSEEPGKSFQTVPN
                     REGGVAESVFIAGQFVLAAEELAALAELLGATEEAVRIRRDRERMIEAIREHGWDGDW
                     FRRAYDFLGKPVGSADSKEGRIWIEPQGMCVMAGVGLDDGRAVRALDAVDEHLATPHG
                     LVLLAPAYSRYHIELGEISSYPPGYKENGGVFSHANPWIAIAEAMVGNGDRAFERYLR
                     INPSAREDLSDLHRCEPYVYAQMIAGKEAATPGEAKNSWLTGSAAWNYVAITQWILGI
                     RPEHGGLRIDPCLPAEWAGFEATRGFRGSTYRIAIHKPRGVTGRITSLLVDGRQVDGD
                     VVPPAPEGSDVTIEGTVA"
     gene            complement(7785..>8223)
                     /locus_tag="VF195_07475"
     CDS             complement(7785..>8223)
                     /locus_tag="VF195_07475"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580695.1"
                     /translation="ADPSAAGLGVVAFSAGMAASRFGGDRLVARFGQPAVARFGASSA
                     FVALAAMLIVHRAVPSVAALGIVGLGLGPVVPLAFRTAGRTPRQGHASALPLVVTAGY
                     AGSIVGPLLVGFMADLATLRIAFLVPVVASAVSALAAGATRDR"
BASE COUNT         1322 a         2754 c         2834 g         1313 t
ORIGIN      
        1 gctggaccac gtgtcgaagg tctacggcgc cgagggaccg agcgcggtct cggacctgaa
       61 cctcgagatc gaggacgggg agttcatcgt cctcgtcggc ccctcggggt gcggcaagac
      121 gaccgctctc cggatggtcg cgggcctcga gtccatcacc gacggaacca tctcgatcga
      181 cgaacgcgtg gtgaacaccg tccccccgaa ggagcgggac atcgcgatgg tgttccagaa
      241 ctacgcgctc tatccgcaca tgaccgtcta cgacaacatg gccttcggcc tcaagctgcg
      301 gaagctctcc aaggaagaga tcgaccggcg ggtacgcgag gccgcgaaga tcctcggact
      361 cgacgacttc ctggaacgca agccgaaggc gctctcgggc ggccagcggc agcgcgtcgc
      421 catgggacgc gcgatcgtcc gcgagcccaa ggcgttcctg atggatgagc cgctgtcgaa
      481 cctggacgcg aagctccggg tccagatgcg ctccgagatc gccaggatcc agcacgatct
      541 gaacgtcacg acactctatg tcacgcacga ccaggtggag gcgatgacga tgggcgatcg
      601 cgtcgcggtg atccgcaagg gcgtgctcca acaggtcgac tccccgcagt tcctctacga
      661 ccatcccgag aacctgttcg tcgccggctt catcggctcg cccgcgatga acatggtcga
      721 ggccgacctg gggcgggaga acggaacctg ggccgtgacg ttcggtggtt tctcgctgga
      781 gatccctgac gaggtgttcg ccgcccggcc ggcgctgcgg gcgttcgagg gcaagcccgt
      841 cgtcatcggg atccgtccgg aggacatgga ggacgcgtca ctcatctcgg acgcgccgcg
      901 gaaccgccgc atccgctcgg cggtgatcct gcgtgaggca ctcggcgccg atgtcctggt
      961 ccacttcggt gtccgggcac gtccggtgat cacggaagac accaaggaac tcgcgcacga
     1021 cgtgggcgcg gaggtcctcg acgccgtgga gcgtgccggc gaccagggcg agtggatctt
     1081 cctcgcccgc ctcagcccga ggacgcaggc gaagcagggc aaggagatcg agctcgtcgt
     1141 ggacacgagc cgattgcact tcttcgaccc cgagacggga cgagggatct acgacggaga
     1201 accggccacc acggaaacgc gtacaacgac atgacgaccc aggcatcgtg cccacgagga
     1261 gaggagatcg gatgcgacgg ctccggatgc tggcgattcc tgccgtgata gccctgatcg
     1321 cggcggcatg caccaccgac gaagacggag gagacggaaa cggaggcggc caggaggaga
     1381 acacgggcac ggtcaacgtc ctgaacgccc tcgagcccga ggagaacgtg gtgctccagt
     1441 cgatctccga caggctgatc gcccccgacg cggactacga ggtggagttc gagcaggtcg
     1501 acgacgtggc ccagcagatc cagatccggg ccgagggagg caccctggat gtcgtgctca
     1561 ccccccaacc gggggcggtc caggagcagg cggcgaacgg gaacgcggtg tcgctggagg
     1621 atcttggctt cgacatcgcc gacctcgagt cgaccttcgg cgagtacttc ctctcgctcg
     1681 gcgagttcga gggcgagcac tacgggatcc cgacgaacat caacctgaag agcatggtgt
     1741 ggtacgcgaa ggacgacttc gacgccgccg gctacacgat cccggagacg tgggacgacc
     1801 tgatcgccct ctccgacgag atggtcgcga acggagacac gccctggtgc gtcggcttcg
     1861 aggcgggtgg cgacaccggg tggcccgcca ccgactggat ggaggacatc gtcctcggca
     1921 gcgccggcac ggagacctac acccagtgga tcaaccacga catcccgttc accgatccgg
     1981 ccgtcgtgca cgccggcgag ctcttcggcg acatgatgtt caccgagggc tacgtgctcg
     2041 gcggggcgga gaacaccccg gcgacggcgt tcgccgacgc gcccgcaccg ctgttcgagg
     2101 acccgcccgg gtgcttcatg caccggcagg cgagcttcat cacggcgttc ttcggcgatg
     2161 ccgaatccgg cgtggactac gactggttcc cgatgcccgg cgagagccag cggccgacac
     2221 tcttcgccgg cgagctcgcc acggtgttct cgaaccggcc ggaggtcgtg gagttcctcg
     2281 aggcattcat gggcgaggag gtccagtgcg cgatggggag cgaaccgggc agctcacgga
     2341 tctcgccgaa cgtcaacgtc ggtccggact gctacacgga cccgatcctc gccgacgcat
     2401 cggaggtgct gaccgctgcc ctcgcgggtg agggcgcccc gggcttcgac gcctccgacc
     2461 agatgccgcc gcaggtcggc tcggggtcgt tctggaccga gatgatgggc tacatggagg
     2521 aaggtccgga ttccctccag acccacctcg agaacatcca ggcgagctgg cccgccgagt
     2581 agcccggacg atggggggat cggtgacggg gggagcaggc ccagagggcc ggctccctcc
     2641 gggccgactc ggaggaggac aggcctagcg tggcgcccga tgacgccgcc ccggcaacga
     2701 tcgacgaacg accgaccgga ccccaagcgg tcctggtctc gatcctgcgg ttgatcggcg
     2761 cgttcgcgat cccgatcgtc ggcttcttca tcctctgggc gacgttcgac ttcctccgcg
     2821 aggcggacgc gaaccgcctg atcgtggtcg gcgtggcgat cctggtcggc gtcggcgggg
     2881 tgttcttcct gtactggggg atgaatcgcg tcgtggactt cctgcccgtg cgctatcggg
     2941 aaggcgtccg gccgtacatc ttcgtcgggc cggcgctcgt gatcctcgcc gtgttcctga
     3001 tctacccggt cctgaacacg atcctggtga gcttcaagga cgctcagggc gagtcgttcg
     3061 tcgggctgga caacttcaga ttcgtcttca ccgacgagag catgctgcgg gcgatccgca
     3121 acacgttcgg atggatcgtc atcgtgccgc tcctgggcgt gacgatcggg ctcgcgttcg
     3181 cgacgctcgc cgaccgcctt cggcgtggtg aagcgatcgc gaagtccatg atcttcctcc
     3241 cgatggcgat ctcgttcgcg ggcgcgtcga tcacgtggag gttgatctac aacttccggc
     3301 cccaagggtt cggctcgaat atcggcctgc tgaacggcat catgctcggg ctcggacagg
     3361 agccggtccg atggctcgac atcgagccgt ggaacaacct gatgctcatg gtgatcatgg
     3421 tgtggatgca gaccgggttc gcgatggtcg tgctgtccgc ggcgatcaag tcgatccccg
     3481 acgagatcct cgaggctgcc cggatcgatg gcgcctccga gctccaggtc ttccggagga
     3541 tcgtcgtgcc cagcatcttg ccgacgatcg cggtggtcac cacgtacatg gtgatcaacg
     3601 cgatgaaggt cttcgacatc gtcttcgtga tggggagccc cgagaccagg ggtacggagg
     3661 tgatcgccga acggatgatc gactggttct tcatcaacaa caacgatggg cgaggggccg
     3721 cgatcgcagt gatcttgttc atcgcgatcg tcccggtgat gatcgtgaac atccggcggt
     3781 tccgcgaaga ggaggcgatc cggtgagcgc cacgacggtc cctccgtcga cccgccctct
     3841 ccccgcccgc ggggagagcc tcgagccccg tggtggcggc tggttcgtcc gcgtcgccgt
     3901 gttcgcggtc gtgatcctct ggatcatccc gacgctcggc gtgctcctca cctcgttccg
     3961 cccggaggcg ctcgtggatt cgaccggctg gtggaccgcg ctcgcgcacc ccttccgaac
     4021 cacggagtgg accctcgaga actaccggct cgccctggac gccggcggct tggggaacgc
     4081 gttcctgaac agcctcgcgg tggcggtccc ggccacggtg atcccgatcg cgatcgccgc
     4141 cttcgcagcc tatgccttct cctggatgga gttccgcggc cgatacgtga tgttcgtggg
     4201 ggtcgtggcg ctgctggtgg tcccgctcca gatggcgctg atcccgatcc tccgtctgta
     4261 caacaacgga ggggtggtcg ggaggtacat catcttcccg gacctcgacc tggtcggcac
     4321 gttcctgggg gtgtggctcg cgcatacggg cttcggtctg ccactcgccg tctatctcct
     4381 tcggaactac atcggctcgc tgccctcctc gatcatcgaa tcggcgaaga tggacggcgc
     4441 cgaccatttc acgatcttct ggcgccttat catcccgctc tcggtgccgg cgctcgcggc
     4501 tttcgcgatc ttccagttcc tctgggtgtg gaacgacctg ctcgtggcct acgtgttcct
     4561 cggtggcacg caacagaacc gcgtgctcac gatcgcgctc gcgaacctcg tcggaggccg
     4621 aggggagaat tggcacctgc tcaccgcggc cgcgttcatc tcgatggcgt tgccgctggt
     4681 cgtcttcttc gctctgcaga agtacttcgt ccgcggtctg acggcgggcg ccgtgaaggg
     4741 ctgagcggat tgtcgcggcc gcgggcgtcc tccgcaccgt ccttcgaacc gctgccgccg
     4801 cggaagaagt ggcgggccat cacgatcgcc accctggcgc tgctcccggc gtacctctcg
     4861 atcgtggtcg ggctggtcgc ggtcgcctcc gaccgggcgg atgccccccc ggcgggtccc
     4921 gcgatcgcgt tcggcctggg actgctgccg ttcgtcttcg tcgcgctcgc gttcctctcg
     4981 tcccatccgc gcgcgcccgg agctgcggcg aaggcgatgg cgctctcgct cctcgtgggc
     5041 atcccggtgg ccgcgctcgt ccccgacgcc gtgacgggtc tcgtggcggg gatcggcgcg
     5101 ggcggagccg cagccctgcg ccccgagcct gatggctcgt ggagagcccg cgcgctgggt
     5161 gtggcagcgg tctcggcgta cgccgccgtc atggtgcgga tcgtgcctga cgtcaccgtg
     5221 ctcctggccc cgaccctccc gttcacgtgc ctcggtgtgg cggatcacct ccgcgaacag
     5281 cgacgggaac gagcgagggg gcgcgaggtc gcagggcgac cttgacacgg ttcggccgct
     5341 tcgacgacgc cgcgcgcgag tacgtcatca cgcgtcccga caccccgctc ccctggatca
     5401 actacctcgg gagcgaggag ttcttctcga tcatctcgaa cacagccggc ggctactcct
     5461 tcttccggga cgcgcggctc cggcgcctca cgcggtaccg ctacaacgcc gcaccggccg
     5521 acaccggcgc ccgcttcctg tacctccgtg acgccgcgac cggcgcgttc tggtcgccct
     5581 cgtggcaacc ggtccgaacc gagctggagg actaccggtg tcgccatggg ctcgggtaca
     5641 cggtgatcgg ctcccggacg tcggggatcg agacggagag cctgtacctc gttcccctcg
     5701 ggtcgccgct cgagatctgg cggacggcag taaccaatcg ccgcgccgac acggcgaggc
     5761 tctccttgtt cggcgccgtc gagttctgcc tctgggacgc gtgggacgac cagacgaact
     5821 accagcggaa cctctcgacc ggcgaggtgc gggtgctgga cggggtcatc taccacacga
     5881 ccgagtatcg agagcgacgg aaccacttcg cctacttcgc gtgctcggag gaggtatcgg
     5941 ggttcgacac gcaacgcgag gagttcctgg ggccgtaccg aggctgggaa cggccggcgg
     6001 tggtggaggt cggtcgatcg cggaactcga tcgccaacgg ctgggcgccc atcgcctcac
     6061 accaggtgga gctcgagctg gcgccgaaag agacgcggga ggtcgtcttc ctgctcggct
     6121 acggggagaa cccaccggat gcgaagttca accctccggg atcgacgagg ctcgaccgcc
     6181 ggcccgtcct cccggtgatc gaacgctacg ggcagcgtgg cgagatcgac gtcgcgctcg
     6241 agcggctgcg cgatcgatgg gccgagctgc tcgacgtcgt gcgggtcacc acaccaaacg
     6301 agcatgtcga ccggatggtg aacgtgtgga acccctacca atgcatggcg acgttcaacc
     6361 tgtcccggtc cgcctcgtcg ttcgacacgg ggatcggacg gggcatcgga ttccgcgatt
     6421 cgagccagga cgtcctgggt tccgtgcaca tggtgcccga gcgtgcgcgt ggacggatcc
     6481 tcgacctggc cgctacgcag ctgccgacgg gcggtgccta ccaccagtac cagccgctca
     6541 cgaagcgagg gaacgacacg atcgggtcgg ggttcaacga cgacccgctc tggctgatcc
     6601 tcgcggtcgc gtcctacgtg aaggagaccg gcgaccggca gatcctggac gaaccggccc
     6661 cgtacgacaa caagcccggg acggagacgc ccctctacga acacctcgag cgggcgctcg
     6721 cgttcacgac cgaccggctc ggcccccacg gcctgccgtt gatcggtcgg gcggactgga
     6781 acgactgttt gaacctgaac gcgttctcgg aggagcccgg caagtcgttc cagaccgtcc
     6841 ccaaccgtga gggcggggtg gccgagtcgg tgttcatcgc ggggcagttc gtcctggccg
     6901 cggaggagct cgcggcgctc gcggagctcc tcggagcgac ggaggaggcc gtccgcatca
     6961 ggcgcgatcg ggagcgcatg atcgaggcga tccgcgagca cggctgggac ggcgactggt
     7021 tccggcgcgc gtacgacttc ctcggtaagc cggtgggatc ggcagacagc aaggaagggc
     7081 ggatctggat cgagccgcag ggcatgtgcg tgatggccgg cgtcgggctg gacgacggcc
     7141 gcgccgttcg cgccctcgac gccgtggacg agcacctcgc gacaccgcac ggcctcgtgc
     7201 tcctcgcgcc cgcgtactcc cggtaccaca tcgagctcgg cgagatcagc tcctacccgc
     7261 cggggtacaa ggagaatggc ggggtcttca gccacgcgaa tccgtggatc gcgatcgccg
     7321 aggcgatggt cggcaacggc gaccgcgcct tcgagcgcta cctgcggatc aacccctccg
     7381 cgcgggagga tctctccgac ctccaccggt gcgagcccta cgtgtacgcg cagatgatcg
     7441 cgggcaagga agcggccaca cccggcgagg cgaagaactc ctggctcacg ggctcggcgg
     7501 cgtggaacta cgtggcgatc acccagtgga tcctgggcat ccgtcccgag cacggcggcc
     7561 tgcggatcga tccgtgcctg ccggcggagt gggcagggtt cgaggccacg cgtggattcc
     7621 gcgggagcac ctaccggatc gcgatccaca agccccgcgg ggtcaccggt cggatcacgt
     7681 cgttgctcgt cgatggccgg caggtcgatg gggacgtcgt ccctcctgct cccgaaggat
     7741 cggacgtgac gatcgagggg accgtcgcat gatccggtga gggctcatcg gtcacgggtc
     7801 gctcccgcgg cgagcgccga caccgcggag gcgacgaccg gcacgaggaa cgcgatccgc
     7861 agcgtggcca ggtcggccat gaagccgacg agcaggggcc cgacgatcga gcccgcatag
     7921 cccgcggtca ccacgagcgg caaggcactc gcgtgcccct ggcgcggggt ccggccggcg
     7981 gtccggaacg cgagaggaac caccgggccg aggccgaggc cgacgatccc gagcgcggca
     8041 acggacggta ccgcgcgatg gacgatcagc atggcggcga gcgccacgaa cgcgctcgag
     8101 gcgccgaacc gagcgacggc gggttggccg aatcgtgcca cgagccgatc cccgccgaag
     8161 cgtgacgccg ccatcccggc cgagaacgcg acgacgccga gtccggccgc gctcgggtcg
     8221 gca
//
LOCUS       DASUYD010000138        25846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_293744, whole genome shotgun sequence.
ACCESSION   DASUYD010000138 DASUYD010000000
VERSION     DASUYD010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25846
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293744"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..233)
                     /locus_tag="VF195_07480"
     tRNA            complement(161..233)
                     /locus_tag="VF195_07480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Arg,seq:cct)
     gene            723..2912
                     /locus_tag="VF195_07485"
     CDS             723..2912
                     /locus_tag="VF195_07485"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580696.1"
                     /translation="MGVAEEEETVPGQRAVTIGTVVAGIVIGAVEVVFAVSFAALVFA
                     GYLEFYFLDDGVGLYLGAAALTLAFMAWRAGRRGVVGGLQGTGAAMMAVVSASVVVHG
                     AGSPNDIFLSALAAIVVAMILCGVVFWWLGSRRRGDLIRFVPYPVVGGFLAGTGWLLF
                     KGGVYVASLVSPFFNPLSDLLAASALQRWLPALAFGVLLLIAMRVITRPLVMPAMIVI
                     GLVVFVVGMVATGSSMDEVRGGGWLLGPFDSAILWKTWTISALGGAGWLSVLESWAGI
                     VTAVFVATFAILFNVSGTELVLDRDLDTNQELRDAGVLNLVSGALGGIPGFHAPSFTA
                     IAERMRVNARIAGLIAGLVPLAAVVFGASVVGLIPRMIVGGVLVFLGLGFIVDWVWDK
                     RRLLPPLEYAVVLLILVAIIAWGFLIGVVIGLVLAVVLFAISYGRIELVHEVAFGETY
                     RSSVDRPATERAELRELTDRVQIMRVSGFVFFGSTNRLLERMRRRTGSTPPRFLVIDL
                     RRVTGVDSSAVVSFVKMLRLAEAAGSEIVFTSASDPVRAQLARGGVVETEGLVAFEPD
                     LDRGLQRCEDALLSPPGPAEARGEHPSAGGPDGLPPGLAAHLERVSVPEGTVLLHQND
                     PPGDIYVLESGRLGVEITTPEGSRIRLRTLRPGVVVGEIALYTSVPRTADVVAETPSV
                     VLRIGRDSIERLEAEEPELAATLHRWLATTLAERLTDTMRTFDVLLD"
     gene            2933..5164
                     /locus_tag="VF195_07490"
     CDS             2933..5164
                     /locus_tag="VF195_07490"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6580697.1"
                     /translation="MVEQRRSSTWLASVVAGLIIGAVESVLAIAFAALVFGGLLAARL
                     PDGIGLYLGAAALTLGFMAWRSGSRGVVGSVQDAAAAVLGLVAAAAAAKAADLAYFAR
                     ISGVKDYEHPDIFLTVVAATLVVTVLCGILFLVIGRFRLGGLIRFVPYPVVGGFLAGT
                     GWLLFKGGIYVATGTLLHLRTVPTMIRQYQLVHWLPALAFGVILLISVRVVKRPLVIP
                     AVIVIGLVVFVAGMVATGSSIEEVREGGWLLFSPSETAQPWKPWALRALGGADWPSVL
                     ESWAGIATAVFVATFAILFNISGTELVLDRDLDTNQEMRGAGVLNLVSGALGGIPGYH
                     ALSLTALAEHMRVDARAAGLVAALVPLAAVVLGGSVVALIPKMIVGGVLVFLGLAFIV
                     EWVWDKRRLLPTREYGVVLVILMGIIVWGFLTGVVIGLVLAVVLFAISYGRIELVREV
                     AFGETYRSNVDRPADERAELHGLSERVQILRVSGFVFFGSTNRLLERIRRRVEEATLP
                     RFLVIDLRRVTGVDSSAVVSFVKVLRLAAASGIEVVFTGASDPVRAQLVRGGVDESEG
                     LASFEPDLDRGLQRCEDALLSAPGRVEAKREHPTAVGPDGPPPGLAAHLERVSLPEGT
                     VLLHKDDPPGDVYVLESGRLDVETVTPEGTRQRLQTLRPGVVVGEITLYTGVARTADV
                     VAEAPSVVLRIGRESIERLEAEEPELAAALHRWLATTMALRLTDRMRALDSILDRGLD
                     RRL"
     gene            complement(5170..5835)
                     /locus_tag="VF195_07495"
     CDS             complement(5170..5835)
                     /locus_tag="VF195_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580698.1"
                     /translation="MTRGSQSTRNKLLPSAMAAASMLLAAIALTWGDAAPAPPPIEPS
                     PSPSPQADGQVLEARTLADLPATLLPYPFTEPAPEPVATEIDGTYMLILTLDDLGGAS
                     HALPFPCRRCLPYARDPGVMTLIFFRGAYFVDHQMSSFHSTGHYEVDGDRLTLFNDPN
                     CSRERGTYTWGLEGGSLRLEAIDDTCAYQGERATDLATEDWTRIPVCRREIAHLWPGI
                     LGC"
     gene            5884..6591
                     /gene="hxpB"
                     /locus_tag="VF195_07500"
     CDS             5884..6591
                     /gene="hxpB"
                     /locus_tag="VF195_07500"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: protein motif:HMM:NF008087.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HEX6580699.1"
                     /translation="MADARPIEAVVFDMDGVLVDSEPMWRAVEREVFAGVGIALTDED
                     LYPTMGVRIADVVDRWYRRHPWPDPSPEQVAETIVDGVARTVREHGLLLDGARDAVDH
                     VRSLGLGVALASSSPMALILAVLSLDGLAARFDVVVSGEDEELGKPDPAVFLSAARRL
                     EVPPERCLVVEDSINGVRAAKAAGMLCVAVPAVGSDADIATEADLVLGSIGELDDRVW
                     VRLGTVPLPSRSMRGDG"
     gene            6588..7418
                     /locus_tag="VF195_07505"
     CDS             6588..7418
                     /locus_tag="VF195_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6580700.1"
                     /translation="MTEIPTITLANGVAMPALGLGTSPMGDRQAEETVSAALRAGYRL
                     IDTAENYRNETGVGRGIRASGVDRDEVFVTTKLNARWHGEAEAQQAWVNSSERLGLER
                     IDLFLIHWPNPEKDRYVEAWRGLLRLLEDGKALAVGVSNFKIAHLDRLLEETGAPPHV
                     NQIQLDPTHSRPEQRAYDAAHGTVTESWSPIGAGGDLLRDPTIAELARRRSKTPAQIV
                     LRWHVQLGLIPIPKTSKPERLDENKEVFDFELDQADMDELASLDRGDEGVMDADRSGH
                     "
     gene            7446..8351
                     /locus_tag="VF195_07510"
     CDS             7446..8351
                     /locus_tag="VF195_07510"
                     /inference="COORDINATES: protein motif:HMM:NF014755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX6580701.1"
                     /translation="MARSVWRGTLSFGLVAIPVSLYPATEAKDVRFHLFDRQGRRVRY
                     RRVVDAGPSGAPLAVDDIGAVAEATEREPAEGRDQPDGAAGTEVAYEDLVRGYEVEPE
                     TYVFLETEEIERVRPSPSRVIDLEHFVRLGDIDPVFFEKAYYVAPGRGAEKSYGLLLR
                     TLKETGRVGIGRFVLRTKPHLVALRPMDDALALETLYFGDEVRDARALVGDVGRAEVT
                     DREIDVSRLLVEALAMEWDPSVFADEYREELLRMIDERTPEHVEGPAEAPVPEARVGE
                     LLEALKRSVEDAKARRKPKGRQRAG"
     gene            8436..8927
                     /locus_tag="VF195_07515"
     CDS             8436..8927
                     /locus_tag="VF195_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019359275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580702.1"
                     /translation="MVSGRIKVLAVAALVPLVMGACSDDVEPSDGTSPPTESSEPSEA
                     PAEATVAVEDSDFGQIVVDAAEGRTLYVFLADTGSDSTCYDDCETNWPPLTVEGDPGA
                     GDGIDASLLGTTEREDGSVQVTLDGHPLYYFAADETPDDTNGQDVGDVWYVVSPEGEA
                     IKG"
     gene            9014..10648
                     /locus_tag="VF195_07520"
     CDS             9014..10648
                     /locus_tag="VF195_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017579801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX6580703.1"
                     /translation="MTVSERIVSQLAAAGVRRAYTVPGESFLPLLDACDRHPDISLIS
                     TRHESGAAFMAEADAKVAGRPALAMATRGVGAANLSIGVHTAYQDSTPMVVILGQVES
                     AFLGREAFQEVDLPSFYREITKWAVTTPSSERLPELVGRAIHVATSGRPGPVMVAVPA
                     DLFEGDAGPEQSHLPGVVDRGRPTPSERTSERIADVLRDADAPVMIVGGGGRDARDAL
                     VTAAERFGLGVYASFRRQDVFPNDHPNYCGPLVLTTPPETLRALEDADIVFVVGCRLS
                     EITTQGYRLPRPDQRVIQIDVEPNSVGAVVPVELGLVADAREALTAILDHAPDASFDR
                     DWTAAHGAFLTASTLPEATDGPLHPAAVIAALAHVFPPWVVVVNDAGNFSIFAHRYWR
                     FAHPRTQIGPTSGAMGYGVPAGIGAKLADPSSEVVVLAGDGGFLMTGQELETAVRHDV
                     PFTTVVFRNGLYGTIALHQARAYGRTVGVDIGHVDIAAVARGYGAEAWSVSGADELTD
                     ALTAARACGRPAVVDCRVDPDVLSPTDRLSRLLADD"
     gene            complement(10623..11867)
                     /locus_tag="VF195_07525"
     CDS             complement(10623..11867)
                     /locus_tag="VF195_07525"
                     /inference="COORDINATES: protein motif:HMM:NF024929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6580704.1"
                     /translation="MRRSLRYGAGGLAGVALGIVLTLAIQARPLPSAPVAPTGPSLLL
                     TRPAPPETFLAWVPHGLPDRFAARVARQPQIGRLTVVAEDNVWLVRSWSAAGELVDRP
                     VSPFRIPLDVAAVDPVTFAPFLPPAHQATLAAIENGEGILGSTSASVRRLGPGAVLEL
                     GGGRRIRIAAVLPDELVGASELLVSKETGRRIGVTRERYLLVRPLATGSMTSASLRRA
                     IQPLLPRDLGVFRRVQARAPGETPYFRAGDAVLPPVLLKALFGEFAARPAPGQPGSLE
                     VDPGWMGTHLVATRIPALGEVLCHTGIVHQLRGAMRELRDAGLDHLIRSFHGCFVPRF
                     ISRDPTALLSHHSWGVAFDVNLVGNYYGQRPHQDERLVCTLARWGFLWGGTFVVPDGN
                     HFEYRRLPKEADPGISRPREAC"
     gene            complement(11864..13846)
                     /locus_tag="VF195_07530"
     CDS             complement(11864..13846)
                     /locus_tag="VF195_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580705.1"
                     /translation="MNCDAVQLVLSARMDGDRVAARQAAAAVAHAETCTRCRTFGERS
                     ARIRSAVRIRAAEPVPDLVEPIMAAVAKERVRPRVPLVGERRVRPPRPTRRPRHRRRT
                     TLAPAAAAAVAGLVLGSVLVGGPWQSAEDRPIAAAAVVRNVRAVAPRLDLFQGTYTIH
                     EFGLSREVPERLFDMEVAFRSPQRFRLEVHDRTAYPSRSWTPTHLRFIQDVTTTFTSG
                     PSGCPGDLPAHVCPRTRAAVTYLSQFSAAAPLPADLVLPVTTFGSATGFEVLGSDRIG
                     DRDAIRVRLSFERAAPMFPFLRMGGTWRPFFERDPVLLWLDARSSLPLRILVHPSRSP
                     DRSAWELRYGLGVESPDEPILDVQLVSVSPEPPSLELFRIPGVSAEPTAPRRLEDLPD
                     RIGYVPATPTDRGDLELVSIVLPPRSAPAAPSSMLVYAEGLDYLKVGERRDWVGPEPF
                     GPLDAGAQQILLDGGGVAYYEPAGEGFGRRLAIHAEGADIFLETNLPRGRLLEIASSL
                     PLVGAPLPPDRSDRGSAGIDVERIDVVEGLTAAGLPASLARNLPDGYVVASAERSTVG
                     SELEGVTLHLRQTDTDAAGEPLTLHVERDTGLPLPSSSEQGLISFAGLEARWTPTRSE
                     LEWVDHGVYHSLQGAMDLRSLIEIAGAIAEVRTGAG"
     gene            complement(13843..14343)
                     /locus_tag="VF195_07535"
     CDS             complement(13843..14343)
                     /locus_tag="VF195_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6580706.1"
                     /translation="MQRARRGDLLAFEDLVREFQADAFRFAWHLTRDRQLAEDVTQEA
                     FLRAFRFLGGFRGDRKFGSWLFSIVRNCAMDALRSSRRSDTPWEDQMASRSVADPTAR
                     AELEAALRSVSTEHREAFLLVEVFGLSYQETADVLGVAVGTVKSRMFRARQALCLAIA
                     DEEEAT"
     gene            complement(14513..15775)
                     /locus_tag="VF195_07540"
     CDS             complement(14513..15775)
                     /locus_tag="VF195_07540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580707.1"
                     /translation="MRSRTILVTLLLLAAVACTTRNERRGTADGPADRPIEDVLVLGA
                     EDGTVTVGSSSGVVLSAGRRQLAAPDGSRLYSTVLGEGSTTLEARDSATGDVVSSTRI
                     RGRLDVRVASLTGRTVALMTPLPPGVDTTYALPRARTSIVVADPAGDSAARRYRLIGN
                     FEPEAFSVDDTRLFLIQYLPAEAPTAYRVTFLDLGSGRVRSVFGRFKTPPERMPGIRL
                     AQVFDPATEQLYTLYTNRAAEYTQGSWADASYGEREVSFVHVLNLRKGWAYCAGLPRA
                     LWGQPARAQALAPSPDGRVLYIVDSMRGRIAMMNTKTLEITGSERIDLGPLEGGRTSA
                     RMSPDGSTLFVGDAGSSLARIDTGTLRMTGRWPLPDDVSGLALSEDGARLYAALGGRV
                     TVVDTDTGDASTTLAFGGIESILHVTTP"
     gene            15936..16703
                     /locus_tag="VF195_07545"
     CDS             15936..16703
                     /locus_tag="VF195_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580708.1"
                     /translation="MRGIRMLFLTTMIGTMALVSPSGAAANGGAYLEFDRTYYVAGDE
                     GHGVTYVSLPARREHLLDQGPFHVFALPRGASLREGHPIPSTAIRLGTVTVTEEDGSY
                     EFETDFIVPDLDPGWYEIGVCNDPCTISGFRESLGGSLSIVETRREARLLTANGRLHS
                     QLLGARREARRAERRLTEVEGELETQLAFGSEERIDLTAEIDRLETQLASARSRAAET
                     GRTPFDPWLVGGILLVTLVIAVLAFRRRRMLPAMTDL"
     gene            16727..17344
                     /locus_tag="VF195_07550"
     CDS             16727..17344
                     /locus_tag="VF195_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580709.1"
                     /translation="MAERYALVPGLDLRPALRTCARVGHTRVPAGVTGAFLEALTEEV
                     DAGPFRAFGESFGPVRQQIDGYDVDIPAPVFPLLTSLCREIRARVRADGRGIRGTATW
                     APNEVGIAHYVPGSIGITPHMDGKWYRRLVVVATVYGRAAFSVCGSRDPDDVVERWIS
                     GPGDLVLMRGPGLAGHRDGRPFHLVHGPMEGERLSLGIRMAGRRD"
     gene            complement(17353..17622)
                     /locus_tag="VF195_07555"
     CDS             complement(17353..17622)
                     /locus_tag="VF195_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580710.1"
                     /translation="MSALEFHEAAWEARATETPPPSPLTLSDRALAIVMAYAEADLPS
                     ETMGMLEDERRRRGMAPTKIERLIGIGMLALIPITAFVFGWILLA"
     gene            17850..18527
                     /locus_tag="VF195_07560"
     CDS             17850..18527
                     /locus_tag="VF195_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580711.1"
                     /translation="MRVLVVEDEKRLAAGIKRGLDAEGFATDVALNGTDGLWMARENP
                     YAAIVLDIMLPGMNGYQVCAALREEEIWTPIMMLTAKDGEFDEAEALDTGADDYVTKP
                     FSYVVLVARLRALIRRGAGERPAVLEAGDLGFDPGSKRTWRAGAEVELTAREMALLEF
                     LLRRKGEVVSKTEILHQVWDYDFEGDPNIVEVYVRHLRNKLDRPFGRNALETVRGSGY
                     RLAADGG"
     gene            18553..19920
                     /locus_tag="VF195_07565"
     CDS             18553..19920
                     /locus_tag="VF195_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011598957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6580712.1"
                     /translation="MRGRLGSIRVRTTVAAVIVVGVALSLASVAMVTVLMRSLRGQVT
                     ATALSKADSLARELESGILLEDLVLGDPNDDEEFVQIVDAGGEVLGSSPNVSGWGPIE
                     LVRPGTTRTIPARSPPDVEPLDDPFLLVASDVDGAGGIESVIVARNLETVIEGRSALT
                     IALVIIMPLLLLVVGLVTWLVTARALSPVDAIRAEVDAISTRDLHRRVPDPPGDDEIS
                     RLAGTMNRMLERLEAGQHRARRFVSDASHELRSPVAAIRQFAEVALAHPERTAVGELA
                     EVVLEEDARLQRLVEDLLLLTRIDEGTIPHPTGAVDLDDLLLEDAVRLRATTDLKVDV
                     RSVSAGRVIGDADQLERLIRNLTDNASRHARGMVRLSLQEVGGRTILCVDDDGIGVDP
                     AEREHIFQRFVRLDDARDRDSGGSGLGLAIVREVATFHGGSVRILDSDLGGIRFEVDL
                     PAQPD"
     gene            20014..20679
                     /locus_tag="VF195_07570"
     CDS             20014..20679
                     /locus_tag="VF195_07570"
                     /inference="COORDINATES: protein motif:HMM:NF024170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEX6580713.1"
                     /translation="MLPEGITGLTSQQIIRLLFSAPTAEAAQRATSSFRSTSPIAFAV
                     PADTELRCRLFLERLRWPHGVYCPRCEDSRRICRIEERAQFECGSCGYQFSVRAGTIL
                     HASHLPLWKWLLAVYLMASADDITSYRLERALGISYKTASHLTHTIRALMGDLSNGVD
                     DAAKVDRHSGRVRGRVERPIDSLPKHADRPTTSGDRLLLVHLLSRLVNAGSGPSDRMV
                     ASA"
     gene            complement(20714..21424)
                     /locus_tag="VF195_07575"
     CDS             complement(20714..21424)
                     /locus_tag="VF195_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580714.1"
                     /translation="MSSRTHARVIAVCIALLPVGGCARFSGFENLASSAACDPASGDF
                     SSDIDNPFFPLPVGHQVVLEGEEGSTHLLVRITSLDETETVAGVETRVVEEFESKDGT
                     VVEISRNFFAQAADGTVCYFGEDVDIFDSGGDVTSHSGEWRAGEGENQPGIFMPPSLE
                     VGVAFEQEIAPGVAEDQARVVAVGESTDVPAGSFDDTVTMEDGSPLDGGTGTKIYARG
                     IGLIVDGPARMTSYSSSS"
     gene            complement(21476..22420)
                     /locus_tag="VF195_07580"
     CDS             complement(21476..22420)
                     /locus_tag="VF195_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580715.1"
                     /translation="MIFDRTRGIHILVLVGGLIASMAACEGTIPPEDNGTGTIATTPS
                     LEPDALLPAGDPPFAIDGNGFDPANFGPDSANVDNTYFPLVPGTQFFYEGSSIEDEGD
                     RIEHSVVFTVTGLTKEIAGVRAVVGWDRDFEEGALVEKELIFFAQDLDGNVWHLGQYA
                     ELYDGKQFEGAAPWLVGYVDGASAGIAMPAEHQEGDPEYSQGYAPPPYYWDDFGRVFK
                     TGQRTCVPAGCYDDVLVTEEFEPLKPGAYQLKFYAHDVGYVRVGWRGAKEREREVLVL
                     TEVRQLEADEMDELHSEAFAMQDRGNVYGLTAPLEVRS"
     gene            complement(22417..25542)
                     /locus_tag="VF195_07585"
     CDS             complement(22417..25542)
                     /locus_tag="VF195_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014496242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6580716.1"
                     /translation="MLRWIVGSSLRFRYIIAAAAAALMFFGVQQLRHMPVDVFPEFAP
                     PRVEIQTLALGLSGTEIENLVTVPLEQALNGVPELDTIRSKSVEQLSQIEMIFERGAD
                     LLTARQLVAERIAAVSPTLPRWAAPPVIIQPLSATSRVMKIGMTSDEYDLVDLSMTAY
                     WKVRAKLLRVPGVANVLIWGERIKMPSVEVDPELLRKHGLTLEEVMTVTSDAVDSGLL
                     RFSEGGFIGTGGFLDTPTNRLQVRHTIPILTGEQLSRVPIVKRGGDVIRLGDVADVVI
                     DTPPLMGDGVINDRPGLLLVVQKLPWANTADVTTGLDAAIADLRPALPQITLDPTIFR
                     PATFIENAIDNLSEAMIIGALLMILMLGAFLYEWRTAVISVTAIPLSLIGAGLVLAWM
                     DVTINTMVLAGLVIALGDIVDDAIIDIENVVRRLREHRAEGGTKSTARVILEASLEVR
                     SAIVYATLIEVVAVVPIFALEGLSGAFFRPLAGAYALALLVSMVIALTVTPAMSLIFF
                     RSGKSLQHRVSPLVPPLQRGYEYLLAKIIYRPRRSYVAVGVTAMLGIGLVPLLGQDLL
                     PSFKERDFLMHWLGKPGVSRPEEVRTTMLVNRELLEIPGVNNAGSHIGQALNSDEPYG
                     IYFGENWVSVDPSVDYDTTLKKIQDTVAAYPGIFRDVQTYLKERIREVLTGSSDAIVI
                     RIFGDDLAVLQEKAQEVEELLGGIPGVVDPFVDTVEDIPQIQIEVDLETARQYGLKPG
                     DVRRSASTLIMGEEVGDLFRNGQTYDVNVWSTPETRENLTDIENLLIDTPSGDYVRLK
                     DVAAIEIQPTPNDIHHEGLFRSIDVSANVQDRDLGSVIGDLEQRLEGVEWPLGYHAEM
                     LGEYAERQAASGRLRLFAIIAALGVFLLLQASFASWRLATLSFVTLPVALIGGVIAAY
                     LGGGIISLGSLVGFFTVLGIVARNGIMLISHYQHLERFEGVAFGPELIIRGARERVVP
                     ILMTVLTTGLVLVPLILAGQIPGNEIEHPMAVVIFGGLITATLLNLFVVPALYLRFGK
                     RGAPGPIAMAP"
     gene            complement(25576..>25846)
                     /locus_tag="VF195_07590"
     CDS             complement(25576..>25846)
                     /locus_tag="VF195_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580717.1"
                     /translation="EDAARRIGLDTVPTTGRGGRTVVPESAIWVDVNGDEWVYTNPET
                     LVFVRAQVVVKRYDDGLAYLSDGPAPGTEVASVGVPELIGSEFGI"
BASE COUNT         3950 a         8650 c         9131 g         4115 t
ORIGIN      
        1 tgaagatcag gctcgagtgg cacgtggagc gggtgggaca caggtgccgg agccggtgtc
       61 gcccgggagc cggagccggg catgagggtg ggacacaggt gccggagccg atgtgggaca
      121 gaggtgccgg aacttcacaa actccgcgaa aactcctcgg tgcccccggc aggaatcgaa
      181 cctgcgacac acggtttagg aaaccgttgc tctctccact gagctacgag ggcgcgaggg
      241 cgcgagggcg agggatgtag acgagggccg agggccgagg gttgtagaga aggtggtcga
      301 gaaccccctt gacagatccg acatccgatc gtgatcgagt ctaccgccgg ggcgagagtc
      361 gatgccgagg acgatcgagt cagcccttgg gtgtcgcttc agcgcgcgca cggcgacgtc
      421 gagcgcgcgg aagttgagga cgacctaggc ggctgaaggg agtctgtcgc gccgttcgtg
      481 ccgaagcacg gtcaagccgt cacgagcaaa gcgtagccga tgaggtccac atgctggcgg
      541 aggcggccga cgctgtgtgc tctcccgagg aagagtgtgt cctcgagatc cctgttgatg
      601 gactcggcat cgttcttggg cgcgtagagg agccgcccga tcggtggccg gatggaccca
      661 ggacaccgag agctgaggac acgactagcg cggtccgggc acccgggcta gggtccacct
      721 ccatgggggt ggccgaggaa gaggaaaccg ttcccgggca acgagccgtc accatcggca
      781 cggtcgtcgc agggatcgtg atcggagccg tcgaggtcgt cttcgcggtt tccttcgcgg
      841 ctctcgtctt cgccggctat ctcgagttct acttcctgga cgacggggtc ggtctgtacc
      901 tcggggcggc cgcgttgacg ctcgcgttca tggcctggcg ggccggccgc cgaggcgtcg
      961 tggggggcct ccagggaacg ggcgcagcca tgatggcggt cgtctcggcg agcgtcgtcg
     1021 tgcacggcgc cgggagtccg aacgacatct tcctcagcgc gctcgccgcg atcgtggtgg
     1081 cgatgatcct gtgcggggtc gtcttctggt ggctcgggtc tcgccggcga ggcgatctga
     1141 tccggttcgt gccgtatccc gtcgtcggag ggttcctcgc cggcacggga tggctgctct
     1201 tcaagggtgg ggtctacgtc gcctcgctgg tatcaccgtt cttcaatccc ctgagcgacc
     1261 ttctggcggc gtcggcgctg cagcgctggc tcccggctct tgcgttcggc gtgctcctgc
     1321 tgatcgccat gcgggtgatc acgcgcccgc tcgtgatgcc ggcgatgatc gtgatcgggc
     1381 tcgtggtctt cgtggtcggg atggtcgcca ccggctcctc gatggatgag gtccgcggcg
     1441 gggggtggct gctcgggcca ttcgactcgg cgatcttatg gaagacatgg acgatcagcg
     1501 ctctcggcgg cgccggctgg ctctccgtgc tcgaatcgtg ggccgggatc gtgaccgcgg
     1561 tcttcgtggc cacgttcgcg atcctgttca atgtcagcgg caccgagctc gtcttggacc
     1621 gggacctcga cacgaaccag gagctccgtg acgcgggcgt cctgaacctc gtgtcgggag
     1681 cgctcggtgg gatccccggc tttcacgcgc cgtccttcac ggcgatcgcg gagcggatgc
     1741 gcgtgaacgc ccggatcgcg ggcctgatcg ccgggctcgt ccccctggcc gccgtcgtgt
     1801 tcggcgcttc ggtcgtgggg ctgatcccgc ggatgatcgt cgggggcgtg ctggtgttcc
     1861 tcgggctcgg gttcatcgtc gactgggtct gggacaagcg gcggttgctc ccgccgctcg
     1921 agtacgccgt cgtgctcctg atcctggtgg cgatcatcgc gtgggggttc ctgatcggtg
     1981 tggtgatcgg cctggtgctc gccgtcgtgc tcttcgccat cagttacggc cggatcgagc
     2041 tggtgcacga ggtcgccttc ggcgagacct atcgctcgag cgtcgatcgg cccgcgacgg
     2101 aacgcgccga gctccgcgag ctcaccgacc gagtccagat catgcgggtt tccgggttcg
     2161 tcttcttcgg atcgacgaac cgcttgctgg agcggatgcg acgtcgcacc ggatccacgc
     2221 cacccaggtt cctggtgatc gatctgcggc gcgtgacggg cgtggattcc tccgcggtcg
     2281 tctcgttcgt gaagatgctg cgactggcgg aggccgccgg atcggagatc gtcttcacga
     2341 gcgcgtccga tccggtgcga gcacagctcg cgcggggagg cgtggtcgag accgaggggc
     2401 tcgtggcctt cgagcccgac ctggatcgag ggctgcagcg gtgcgaggac gccctgctga
     2461 gtcctcccgg accggcggag gcgcgggggg agcatccctc cgcgggcggc cccgacggtc
     2521 tgccaccggg cctcgccgcc cacctcgagc gcgtctcggt gcccgagggc acggtgctgc
     2581 tgcaccagaa cgacccgccg ggcgacatct acgtgctcga gtcggggcgc ctcggcgtgg
     2641 agatcacgac gcccgagggc agcaggatcc gcctgcggac gctccgtcct ggggtcgtgg
     2701 tcggcgagat cgcgctctac acgagcgtcc cgcgaaccgc cgacgtggtg gccgagaccc
     2761 cgagtgtggt cctgcgcatc ggccgcgatt cgatcgagcg gctcgaggcc gaggagccgg
     2821 agctcgccgc gacactccac cggtggctcg ccacgacact ggcagagcgg ctgaccgaca
     2881 cgatgcgaac gttcgacgtg ctgctggatt gacggcaagg ggggagacac ggatggtcga
     2941 acagcgaagg tcctccacgt ggctcgcctc ggtcgtcgcc ggtctgatca tcggcgcggt
     3001 cgagtcggtg ctcgcgatcg cgttcgccgc cctcgtcttc ggcggcttgc tcgcggcccg
     3061 ccttccggac ggcatcggcc tgtacctcgg agcggcggcg ctgacgctcg ggttcatggc
     3121 ttggcggtcc ggctcccgcg gggtcgtcgg cagcgtgcaa gacgcggcgg cggccgtcct
     3181 cgggctcgtc gcggccgccg cggcggcgaa ggccgccgac ctcgcgtatt tcgcacggat
     3241 atccggtgtg aaggactatg agcatccgga catcttcctg accgtcgtcg ccgcgaccct
     3301 cgtggtgacg gtcctgtgcg ggatcttgtt cctggtgatc gggaggttcc ggctcggcgg
     3361 gttgatccgg ttcgtgccgt atccggtcgt cggagggttc ctcgccggca cgggatggct
     3421 gctcttcaag ggtgggatct acgtcgccac ggggacgctc ctgcacctgc gcacggtccc
     3481 cacgatgatc cgccagtacc agctcgtcca ctggctcccg gccctcgcgt tcggcgtgat
     3541 cctgctcatc tcggtccggg tcgtgaagcg gccgctcgtg atcccggcgg tgatcgtgat
     3601 cgggctcgtg gtgttcgtgg cggggatggt cgccaccggc tcctcgatcg aggaggtccg
     3661 cgaagggggg tggctgctgt tctcaccgtc cgagacggcg cagccgtgga agccgtgggc
     3721 gcttcgcgcg ctcggtggcg ccgactggcc ctccgtgctc gaatcgtggg ccgggatcgc
     3781 gaccgccgtg ttcgtggcca cgttcgcgat cctgttcaac atcagcggca ccgagctcgt
     3841 cttggaccga gacctcgaca cgaaccagga gatgcgcggt gccggggtcc tgaacctcgt
     3901 gtcgggggcg ctcggtggga tccccggcta tcacgcgctc agcctcacgg cactcgcgga
     3961 gcacatgcgc gtggacgcgc gggcggctgg gctcgtcgcg gcactcgtcc ctctggccgc
     4021 cgtcgtgttg ggcggttcgg tcgtcgcgct gatcccgaag atgatcgttg gaggcgtgct
     4081 ggtgttcctc gggctcgcct tcatcgtcga gtgggtctgg gacaagcggc ggctgctacc
     4141 cacgcgcgaa tacggagtgg tgctcgtgat cctgatgggg atcatcgtct gggggttcct
     4201 gaccggggtg gtgatcggcc tggtgctcgc ggtcgtgttg ttcgcgatca gctacggccg
     4261 gatcgagctg gtccgcgagg tcgccttcgg cgagacctat cgcagcaacg tcgatcgccc
     4321 ggccgacgag cgcgccgagc tccacgggct ctccgagcgc gtgcagatcc tgcgggtttc
     4381 cgggttcgtc ttcttcggat cgaccaaccg cctgctggag aggatccgac ggcgcgtaga
     4441 ggaggccacg ctgccccggt tcctggtgat cgatctgcgg cgcgtcacgg gcgtggactc
     4501 ctcggcggtc gtctcgttcg tgaaggtgct ccgcctggcg gcggcgtccg gcatcgaggt
     4561 cgtgttcacg ggcgcctccg acccggtgcg cgcacagctg gtccggggag gtgtggacga
     4621 gtcggagggg ctcgcgtcct tcgagcccga cctggaccga gggctgcagc gatgcgagga
     4681 cgcgctgctc tccgcccccg gtcgggtcga ggcgaaacga gagcacccca ccgccgtcgg
     4741 ccccgacgga ccgccaccgg gcctcgccgc ccacctcgag cgcgtctcgt tgcccgaggg
     4801 cacggtcctg ctccacaaag acgacccgcc gggcgacgtc tacgtgctcg agtccggacg
     4861 cctggacgtg gagaccgtca cgcccgaggg cacgcggcag cggctgcaga cgctccgccc
     4921 tggggtcgtc gtgggcgaga tcacgctcta tacgggggtc gctcgcaccg ccgacgtggt
     4981 ggccgaggcc ccgagcgtgg tcctgcgcat cggccgcgag tcgatcgagc ggctcgaggc
     5041 cgaggagccg gagctcgccg cggcgctgca ccggtggctc gccacgacga tggcgctccg
     5101 cctcacggac cggatgcgag cgctggactc gatcctggat cgaggcctgg atcgaaggct
     5161 ctgaccaact cagcaaccga ggatgccggg ccacaggtgc gcgatctcgc gcctgcacac
     5221 cgggatccgc gtccagtcct ctgtcgcgag atcggtcgcc cgctccccct ggtacgcgca
     5281 cgtgtcgtcg atggcctcga ggcgcagcga tccgccctcc agtccccagg tgtacgttcc
     5341 gcgctcccgg gagcagttcg ggtcgttgaa cagggtgagc cggtcgccgt ccacctcgta
     5401 gtgcccggtc gaatggaacg aggacatctg gtggtccacg aagtatgcgc cccggaagaa
     5461 gatcagggtc atcaccccgg ggtcgcgagc gtacgggagg caccgtcgac acgggaacgg
     5521 cagcgcatgg ctcgcgcctc cgagatcgtc gagcgtgagg atcagcatgt acgttccgtc
     5581 gatctcggtg gcgacgggct ccggcgccgg ctccgtgaac gggtagggga gcaaggtcgc
     5641 gggcaggtcc gcgagcgtcc tcgcctccag cacctggcca tcggcctggg gtgacggcga
     5701 gggggagggc tcgatcggag ggggagccgg cgcggcgtcg ccccaggtca gcgcgatcgc
     5761 cgcgagcagc atcgaggcgg cggccatcgc ggacgggagg agcttgttcc tcgtcgactg
     5821 cgatcctcgg gtcacgggca gtgggtgatg atacgcggcg cgcccgtagc atccgttgcc
     5881 gtgatggcgg acgcgcgacc gatcgaggcc gtcgtcttcg atatggacgg cgtgctggtc
     5941 gactcggagc ccatgtggcg cgcggtggag cgcgaggtgt tcgcgggcgt cggtatcgcg
     6001 ctgacggacg aggacctgta tccgacgatg ggcgtccgga tcgccgacgt cgtggaccgc
     6061 tggtatcggc ggcacccgtg gcccgatccc agccctgagc aggtggcgga gacgatcgtc
     6121 gacggagtcg cccgcaccgt ccgtgagcac ggcctcctgc tcgacggtgc gcgcgacgcg
     6181 gtcgaccacg ttcggtcgct cgggcttggg gtcgcgctcg cgtcgtcgtc gccgatggcc
     6241 ctgatcctgg cggtgctctc cctcgacggg ctcgccgcgc ggttcgacgt cgtggtctcc
     6301 ggcgaggacg aggagctcgg caagcccgat cccgccgtgt tcctgtccgc ggcgcggcgg
     6361 ctggaggtcc cgccggagcg gtgtctcgtc gtggaggact cgatcaacgg cgtccgggcg
     6421 gcgaaggccg cagggatgct ctgcgtcgcg gtgccggccg tcgggagcga cgccgatatc
     6481 gcgaccgagg cggatctcgt cctcggatcg atcggagagc tcgacgaccg ggtgtgggtg
     6541 cgcctcggca cggtcccgct accatcccga tcgatgcgag gcgacggatg actgagatcc
     6601 ccacgatcac gctcgccaac ggggtcgcga tgcccgcgct gggcctcggc acctcgccga
     6661 tgggcgaccg gcaggccgag gagaccgtct cggccgcgct ccgagccggc taccggctga
     6721 tcgacaccgc cgagaactac cggaacgaga ccggcgtggg ccgggggatc cgggcgagcg
     6781 gcgtcgatcg cgacgaggtg ttcgtcacca cgaagctgaa tgcgcgttgg cacggcgagg
     6841 cggaggcgca gcaggcgtgg gtgaacagct cggagcgcct cggcctcgag cgcatcgacc
     6901 tgttcctgat ccactggccc aatcccgaga aggaccgcta cgtggaagcg tggcgcggcc
     6961 tcctccggct cctcgaggac ggcaaggccc tcgcggtcgg cgtctccaac ttcaagatcg
     7021 cccacctcga tcgactgctc gaggagaccg gcgccccgcc gcacgtgaac cagatccagc
     7081 tggatccgac gcacagccgg cccgagcagc gtgcctacga cgccgcgcac gggaccgtga
     7141 ccgagagttg gtcgccgatc ggcgcaggcg gcgatctgct ccgagacccg acgatcgccg
     7201 agctggcccg tcgtcgctcg aagaccccgg cgcagatcgt gctccggtgg cacgtccagc
     7261 tcggactcat cccgatcccc aagacctcga aacccgagcg gctggacgag aacaaggagg
     7321 tcttcgactt cgagctcgac caggccgaca tggacgagct cgcttccctg gaccgaggcg
     7381 acgaaggcgt catggacgcc gaccgatccg gtcactgacg ggcgctccct cggtacgctg
     7441 agcccatggc gcggtcggtg tggcgcggga cgctcagctt cgggctcgtc gcgatcccgg
     7501 tgtccctcta tcccgcgacc gaggcgaaag acgtgcggtt ccatctgttc gacaggcagg
     7561 gacggcgcgt ccggtaccgc cgggtcgtgg acgcggggcc gtcgggagct ccgctggcgg
     7621 tcgacgacat cggagccgtg gccgaggcga cggagcggga acctgccgag ggtcgggacc
     7681 agccggacgg tgcggccggg accgaggtgg cctacgaaga tctcgtccgg ggctacgagg
     7741 tcgagcccga gacctacgtc ttcctcgaga ccgaggagat cgagcgcgtc cgcccctccc
     7801 cgagccgcgt gatcgacctc gagcatttcg tccggttggg ggacatcgac ccggtgttct
     7861 tcgagaaggc gtactacgtg gctcccgggc gcggcgcgga gaagtcctac gggctgctcc
     7921 tgcggacgtt gaaggagacg gggcgcgtgg gtatcgggcg gttcgtcctc cggacgaagc
     7981 cgcacctcgt ggcgctccgg ccgatggacg atgcgctcgc cctcgagacc ctgtacttcg
     8041 gtgacgaggt gcgcgatgcc cgggcgctcg tgggggatgt cggacgtgcc gaggtgacgg
     8101 atcgcgagat cgacgtgtcg aggctgctcg tcgaggcgct cgcgatggag tgggacccct
     8161 ccgtcttcgc cgacgagtac cgggaggagc tgctgcgcat gatcgacgag cgcacgcccg
     8221 agcacgtcga gggaccggcc gaggccccgg tgcccgaagc gcgcgtgggt gagctcctgg
     8281 aggccctcaa gcggagcgtc gaagacgcca aggcgcggcg gaagcccaag ggtcggcagc
     8341 gggccggcta ggcacgaccc agccgaagtg ctcctggaac gaaccggagc tcccgcccac
     8401 ccgtagacct tccgacgacg acgaaggagg ctcggatggt gtctggccgg atcaaggtgc
     8461 tcgcggtggc cgcgctcgtc ccgctcgtga tgggcgcgtg ctcggatgac gtcgagccct
     8521 cggacggcac gtccccgcca accgagtcat cggagccgtc cgaggcgccc gccgaggcga
     8581 cggtcgccgt cgaggattcc gacttcgggc agatcgtggt cgatgccgcc gagggacgca
     8641 cgctctacgt gttcctcgcc gacacgggca gcgacagcac gtgctacgac gactgcgaga
     8701 ccaactggcc gccgctcacc gtggagggtg atcccggcgc cggcgatggt atcgacgcct
     8761 cgctcctcgg caccacggag cgtgaggacg ggagcgtcca ggtcacgctc gacggccacc
     8821 cgctgtacta cttcgccgcc gacgagacgc cggacgacac caacggacag gacgtcggcg
     8881 acgtctggta cgtggtgtcc cccgaaggtg aggccatcaa ggggtagctc cgcggggctc
     8941 ctcccgcacg ccctttgccg gtcccggctg cggcgtatcc tccccgtctt cggcatggga
     9001 ggcggcggga cgcgtgacgg tatcggagcg gatcgtttcg cagctcgccg cggccggcgt
     9061 ccggcgcgcc tacacggtcc cgggcgagag cttcctcccg ctgctcgatg cctgcgaccg
     9121 tcatccggac atctcgctga tctccacgcg gcacgagtcc ggcgccgcgt tcatggcgga
     9181 ggccgacgcg aaggtcgccg gacggccggc gctcgccatg gccacgcggg gcgtcggggc
     9241 ggcgaacctt tcgatcggcg tgcacacggc ctaccaggac tcgacgccga tggtcgtgat
     9301 cctcgggcag gtggagtcgg cgttcctcgg gcgggaggcg ttccaggagg tcgacctccc
     9361 atcgttctac cgggagatca cgaagtgggc cgtgacaacc ccgtcgagcg aacggctgcc
     9421 ggagctggtc ggcagggcga tccacgtcgc gacgagtggg cggccgggtc cggtgatggt
     9481 cgccgtgccc gcggacctct tcgagggtga cgcaggcccg gagcagagcc acctccccgg
     9541 cgtggtcgac cggggcaggc ccacgccgag cgaacggacc agcgagcgga tcgcggatgt
     9601 cctccgagac gcggacgcgc ccgtgatgat cgtcggcggc ggggggaggg acgcgcggga
     9661 cgcgctcgtc acagccgcgg agcggttcgg cctgggcgtc tacgcgagct tccgccgcca
     9721 ggacgtcttc cccaacgacc atccgaacta ctgcggccca ctcgtgctca ccaccccgcc
     9781 ggagacgttg cgggccttgg aggacgccga catcgtgttc gtcgtcggct gtcggctgag
     9841 cgagatcacg acccagggct accggctgcc gaggcccgat cagcgggtga tccagatcga
     9901 cgtggagccg aacagcgtcg gtgcggtcgt gccggtggag ctcggtctgg tcgcggacgc
     9961 gcgcgaggcc ttgacggcga tcctcgacca cgctccggac gcatcgtttg accgcgactg
    10021 gaccgcagcg cacggcgcgt tcctcacggc gagcaccctc ccggaggcga ccgacggccc
    10081 gctccacccg gcggccgtga tcgcggccct cgcgcacgtg ttcccgccct gggtcgtcgt
    10141 cgtgaacgac gcggggaact tctcgatctt cgcgcaccgc tactggcggt tcgcgcatcc
    10201 acgcacccag atcggaccca cgagcggcgc gatggggtac ggcgtcccgg ccggcatcgg
    10261 cgccaagctc gcggatccgt cgagcgaggt cgtcgtcctg gcgggtgacg gtggattcct
    10321 gatgacgggc caggagcttg agacggcggt ccgccacgac gtgccgttca ccaccgtcgt
    10381 gttccgcaac ggcctctacg gcacgatcgc gttgcaccag gcgcgcgcct acgggcgcac
    10441 ggtgggggtc gacatcggcc acgtggacat cgccgcggtg gcccgcgggt acggcgccga
    10501 ggcctggtcc gtctcgggcg ccgacgagct caccgacgcc ctgaccgcag ctcgggcctg
    10561 cggccggccg gccgtggtcg actgccgggt ggatccggac gtcctctcgc ccaccgatcg
    10621 cctcagcagg cttctcgcgg acgactgatc ccgggatcag cctccttcgg caaccgccgg
    10681 tactcgaagt ggttgccgtc gggcaccacg aacgtcccac cccacaggaa cccccaccgc
    10741 gcgagcgtgc agacgagccg ctcgtcctgg tgaggtcgct gaccgtagta gttgccgacg
    10801 aggttcacgt cgaaggccac accccaggag tgatgggaca ggagcgccgt cgggtcgcgg
    10861 ctgatgaacc tgggcacgaa gcagccgtgg aaggaccgga tcagatgatc caggcccgca
    10921 tcccgcagct cccgcatcgc gccgcggagt tggtgaacga tccccgtgtg gcacagcacc
    10981 tctccgaggg ccgggatccg ggtcgccacg aggtgcgttc ccatccatcc tgggtcgacc
    11041 tcgagggagc caggctgtcc cggcgccgga cgggccgcga actcgccgaa gagcgccttc
    11101 agcaggaccg gggggaggac ggcgtcgccc gcccggaagt acggcgtctc gccgggtgcc
    11161 cgggcctgga cgcgacggaa gacgcccagg tcccgaggga ggagcggctg gatcgctcgc
    11221 cggagtgatg cggatgtcat cgacccggtg gcgagcgggc ggaccaggag gtagcgctca
    11281 cgcgtgaccc cgatccggcg gcccgtctcc ttcgacacga ggagctccga tgccccgacc
    11341 agctcgtccg gcaggaccgc cgcgatccgg atccgcctcc ctccgccgag ttccagcacc
    11401 gcgccgggtc ccagccgtcg gacggaggcg gaggtcgatc cgaggatccc ctcgccgttc
    11461 tcgatcgccg cgagcgtcgc ctggtgggcc gggggaagga acggcgcgaa cgtcacaggg
    11521 tcgacggcgg cgacgtcgag cgggatccgg aatggactca cggggcggtc gacgagctcg
    11581 cccgcggcgc tccatgagcg gacgagccac acgttgtcct ctgcgacgac ggtgagcctt
    11641 ccgatctgcg gttgccgagc cacccgcgcg gcgaagcggt cgggaagacc gtgcggcacc
    11701 cacgcgagga acgtctccgg cggcgccggt cgcgtgagga gcaacgacgg ccccgtcggc
    11761 gccacgggcg cggaggggag gggtcgcgcc tggatcgcga gggtgagcac gatgccgagt
    11821 gcgaccccgg cgaggccgcc ggcgccgtat cgcagcgagc gtctcatccg gcccctgtcc
    11881 tcacctcggc gatcgcgccg gcgatctcga tcaacgatcg gagatccatc gctccctgga
    11941 gcgagtgata gacgccatgg tcgacccatt cgagctccga tcgggtcggc gtccatcggg
    12001 cttcgagacc cgcgaacgag atgagtccct gttccgacga cgagggcagc ggcagcccgg
    12061 tgtcgcgctc gacgtgcagg gtcaacggct cgcccgccgc atcggtatcg gtctggcgga
    12121 ggtggagcgt gacgccctcc agctcggacc cgacggtgga gcgttcggcg ctcgcgacga
    12181 cgtagccgtc cgggaggttc cgagccaggg atgcgggcag gcccgccgcg gtgagtccct
    12241 cgaccacgtc gatccgctcg acgtcgatgc cggcggaccc tcgatcgctc cggtccggag
    12301 ggagcggagc accgacgaga gggagggagg acgcgatctc gagcaaccgc ccgcgaggga
    12361 ggttcgtctc gaggaagatg tcggcgccct ctgcatggat cgcgagccgg cggccgaagc
    12421 cctctcccgc gggttcgtaa tacgcgaccc cgccgccgtc gaggaggatc tgctgcgcgc
    12481 ccgcgtccaa aggtccgaac ggctcggggc cgacccaatc ccgtcgctcg ccgaccttca
    12541 gatagtcgag tccctccgcg tacacgagca tcgagctggg cgccgccggc gcggaccgcg
    12601 gggggagaac gatcgagacg agctccaggt cgccgcgatc cgtcggggtc gccgggacat
    12661 acccgatgcg gtccgggagg tcttccagcc gacgtggcgc cgtcggttcc gcagagacac
    12721 cggggatccg gaagagctcg aggctgggag gctccggcga gacggagacg agctgcacgt
    12781 cgaggatcgg ctcatcgggc gactccacgc cgaggccgta gcggagctcc catgcgctgc
    12841 gatcggggct ccgcgacgga tgcacgagta tccggagcgg cagcgaggag cgggcgtcga
    12901 gccacaacag gacgggatca cgctcgaaga agggcctcca cgtcccgccc atccgcagga
    12961 acgggaacat cggagctgcc cgctcgaagg agaggcggac gcggatcgcg tcccggtcgc
    13021 cgatccgatc cgacccgagc acctcgaacc ccgtcgcgga tccgaacgtg gtgaccggga
    13081 gcacgaggtc tgccggcaag ggggccgccg cggagaactg ggagaggtat gtcactgcgg
    13141 cgcgcgtccg ggggcacacg tgggcgggga ggtcgccggg gcaccccgag ggacccgacg
    13201 tgaacgtcgt cgtcacgtcc tggatgaagc ggaggtgggt cggggtccag gaacgggacg
    13261 ggtaggcagt ccggtcatgg acctcgaggc ggaaacgttg cggggaacgg aaggccacct
    13321 ccatgtcgaa gagacgttcc ggcacctcgc gcgacaggcc gaactcgtgg atcgtgtagg
    13381 tgccctggaa gaggtcgagt ctcggcgcca ctgctcgcac gttccgcacg acggctgccg
    13441 ccgcgatcgg ccgatcctcc gcgctctgcc acggtcctcc caccagcacg ctgccgagga
    13501 ccaggccggc gaccgccgcg gcggcggcgg gcgcgagggt ggttcgacga cggtggcgcg
    13561 gtcgtcgggt gggtcggggt ggtcggacgc gtcgctcgcc cacgagggga accctcggtc
    13621 gaacccgctc cttcgcgacg gcagccatga tcggctccac caggtcgggc accggctcgg
    13681 cggcgcggat ccgcaccgcg ctgcggatcc gcgcggatcg ttcgccgaag gtccggcagc
    13741 gggtgcacgt ctcggcgtgc gcgacggcgg cggccgcctg tcgcgcggcc acgcgatcac
    13801 cgtccatccg ggcggacagg acgagctgca ccgcgtcgca gttcacgtcg cctcctcctc
    13861 gtccgcgatc gcgaggcaga gcgcctgccg cgcccggaac atcctgctct tcaccgtgcc
    13921 cacggcgacg ccgaggacgt cggccgtctc ctggtacgac agcccgaaca cctcgacgag
    13981 gaggaaggcc tcccgatgtt ccgtcgagac cgatcggagg gccgcctcga gctccgcgcg
    14041 agccgtgggg tcggcgacgc tcctcgaggc catctggtct tcccacggcg tgtcggacct
    14101 ccgactgctg cggagcgcgt ccatcgcgca gttccgcacg atgctgaaca gccacgaccc
    14161 gaacttccgg tccccccgga agcccccgag gaaccggaag gcgcggagga aggcctcctg
    14221 ggtgacgtct tccgccagct ggcggtcacg tgtgaggtgc cacgcgaaac ggaaagcatc
    14281 cgcctggaac tcgcggacga gatcctcgaa cgcgaggagg tcgccgcggc gcgcacgctg
    14341 caccgttcgc ggatccggat cccgcacccg cttcctctca gctcgtcccg acacggcagc
    14401 gagggcgccg gcggcgcccc acgcgccggg tcgggggtcc ggcgctcacg atgcgccctc
    14461 gtccgcccat cggcaaggag acgggcgcca cctcccacgg gttccatcgg gcctacggcg
    14521 tcgtgacgtg caggatcgat tcgatcccgc cgaacgcgag cgtcgtgctc gcatcaccgg
    14581 tgtcggtgtc cacgacggtg acccggcctc cgagggcggc gtagaggcga gccccgtcct
    14641 ccgacaacgc gagcccgctc acgtcgtcgg gcaacggcca gcgcccggtc atccggagcg
    14701 tcccggtgtc gatgcgtgcc agcgacgacc ccgcgtcgcc gacgaacagc gtgctcccgt
    14761 cggggctcat ccgcgccgac gtgcgcccac cctcgagcgg tcccagatcg atccgctcgg
    14821 atccggtgat ctcgagggtc ttcgtgttca tcatggcgat ccgtcctcgc atcgagtcga
    14881 cgatgtagag gacacgaccg tccggagacg gcgcgagagc ttgcgctcgc gccggttgcc
    14941 cccatagcgc tcgtgggaga ccggcgcagt acgcccatcc cttccggagg ttcaggacgt
    15001 ggacgaagct cacctcacgc tcgccgtacg acgcgtccgc ccaggagccc tgcgtgtact
    15061 cggcggcgcg attcgtgtac agcgtgtaga gctgctcggt ggccgggtcg aacacctgcg
    15121 cgaggcggat gccgggcatg cgctccggcg gcgtcttgaa ccggccgaac accgagcgga
    15181 cacggccgct cccgaggtcg aggaaggtga cccggtaggc ggtcggcgcc tcagccggga
    15241 ggtactggat gaggaacagg cgagtgtcgt cgaccgagaa ggcctccggc tcgaagttcc
    15301 cgatcagccg gtagcggcgt gcggcgctat cgccggccgg atcggccacg acgatcgagg
    15361 tgcgcgcgcg cgggagtgcg taggtggtat cgacgcccgg cgggagtggg gtcatcaagg
    15421 cgacggtgcg acccgtcaac gacgcgaccc gcacatcgag tcggcctcgg atcctcgtgc
    15481 tggagacgac atcaccggtg gccgagtccc gcgcttcgag cgtcgtcgaa ccttcgccga
    15541 ggacggtcga atacagcctg gaaccgtccg gcgccgccag ctggcgccga ccggcggaca
    15601 gcacgacgcc ggaggacgaa ccgacggtga ccgttccgtc ctcggccccg aggacgagga
    15661 cgtcctcgat cggacgatct gccggtccgt ccgcggtgcc tcgtcgttcg ttccgggtgg
    15721 tgcacgcgac cgcggcaagc aggagcaacg tgacgaggat cgtgcgggag cgcatcgcgc
    15781 tacctccttc cgggccgccc ggcgcacgtg gtctcggtcg ggcggctcat cccatgtacc
    15841 ccgggcgtcc gcccgaagtt cccccgaacg acgcggaacc ttcggagatc cggcccggtc
    15901 tctcgcgtgg acgttcgaac agaggaggtc gcacgatgcg tgggatccgg atgctgttcc
    15961 tgacgacgat gatcggcacg atggcgctgg tctcgccatc cggcgcggcc gcgaacggcg
    16021 gcgcgtacct ggagttcgat cgcacgtact acgtggcggg cgatgaggga cacggtgtca
    16081 cgtacgtgtc gctccccgcg cgcagagagc atctccttga ccagggtccc ttccacgtct
    16141 tcgcgctgcc gcgcggggca tcgctccgcg agggtcatcc gatcccgtcg accgcgatcc
    16201 gcctcgggac cgtgaccgtg accgaggaag atgggtccta cgagttcgag acggacttca
    16261 tcgtgcccga tctcgatccg ggctggtacg agatcggtgt gtgcaacgat ccgtgcacga
    16321 tctcagggtt ccgcgagtct ctcggcgggt cgctctcgat cgtcgagacg cgtcgggagg
    16381 cccgactgct gaccgcgaac ggacggctcc acagtcagct gctcggcgct cgccgtgaag
    16441 ctcggcgagc ggagcgccgc ttgacggagg tggagggcga gctcgagacg caactcgcgt
    16501 tcgggagcga ggagcggatc gacctgaccg ccgagatcga ccggctcgag acgcagctgg
    16561 cctccgctcg gagccgcgca gcggagacgg gacggacgcc gttcgatccg tggctcgtgg
    16621 gcgggatcct gctcgtgacc ctcgtcatcg cggtgctcgc gttccggcgc cggcggatgc
    16681 tgcccgcgat gacggacctc tagcgcgcgc ggcatcatcg ggggtcatgg cggagaggta
    16741 tgcactcgtt cccggcctcg atctccgacc cgcgctccgt acgtgtgccc gggtcgggca
    16801 cacgcgggtt ccggcgggcg tcaccggcgc gttcctcgag gcgctcacgg aggaggtcga
    16861 cgccggtcca ttccgggcgt tcggggagtc gttcgggccg gtccggcagc agatcgacgg
    16921 ctacgacgtc gacatccccg ccccggtctt cccgctgctc acgagcctgt gcagggagat
    16981 ccgagcacgc gtcagagcgg atggccgagg gatccgaggg acggccacgt gggcaccgaa
    17041 cgaggtcggg atcgcacact acgtgccggg ctccatcggc atcacgccgc acatggacgg
    17101 gaagtggtac cgacggctcg tggtcgtggc caccgtctac ggtcgagcgg cgttctcggt
    17161 ctgcggatca cgcgacccgg acgacgtcgt cgagcgatgg atctccggtc ccggtgacct
    17221 cgtgctgatg cgcgggccgg gcctcgccgg ccaccgcgac ggtcgtccgt tccatctcgt
    17281 gcacggcccc atggaagggg agcggctctc gctcgggatc cggatggcgg ggcgacggga
    17341 ctgagggcgc tctcaggcga ggaggatcca tccgaacacg aacgcggtga tggggatgag
    17401 cgccagcatc ccgatcccga tcagtcgttc gatcttggtt ggcgccatgc cgcgacgtcg
    17461 acgctcgtcc tcgagcatcc ccatcgtctc ggaagggagg tcggcctccg cgtacgccat
    17521 cacgatcgcg agcgcccggt cggaaagcgt caagggggac gggggcgggg tctcggtcgc
    17581 cctcgcttcc caggccgcct catggaactc cagggcgctc atgtcagctc caacgagcac
    17641 agactcagcg ttctcggtca tcgcgaaccc cccggttcgt cgggaccctc cgcttcccgt
    17701 gctacccccg atcgtgagtg tcggaaaccg gtcctgaagg gaaactgaac gtgattcgtg
    17761 gcggtgtttc ccttcagagc gcctcctcga ttcagctggt gctcaggatg gcgtggcaac
    17821 gataggagca tcgggagagg agttggggga tgcgcgtgct cgtggtggaa gacgagaagc
    17881 ggctcgccgc tgggatcaag aggggcctgg acgccgaagg cttcgcgacc gatgttgcgc
    17941 tcaacggcac cgacgggctc tggatggcgc gcgagaatcc ctacgccgcg atcgtgctcg
    18001 acatcatgct tccggggatg aatggctacc aggtgtgcgc cgcgctccgt gaagaggaga
    18061 tctggacccc gatcatgatg ctcacggcga aggacggaga gttcgacgag gcggaagcgc
    18121 tcgacaccgg tgcggatgac tacgtgacga aacccttctc ctacgtcgtc ctcgtcgctc
    18181 gtctccgagc gctgatcagg cggggagccg gcgagcgacc cgccgtcctg gaggccggtg
    18241 acctcggctt cgaccccgga tcgaaacgga cgtggcgagc gggagccgag gtcgaactca
    18301 ccgcccggga gatggcgctc ctggagttcc tgctccggcg gaagggagag gtcgtgtcga
    18361 agacggagat cctccaccag gtgtgggact acgatttcga gggcgacccc aacatcgtgg
    18421 aggtctacgt ccggcatctc aggaacaagc tggatcgacc cttcgggcgg aacgcactcg
    18481 agacggttcg cgggtccggt tatcggttgg cggctgatgg tgggtagcga gccagcggtg
    18541 agatcgccgg ggatgagagg ccgcctcggg tcgatccggg ttcggacgac cgtggccgct
    18601 gtgatcgtgg tcggcgtggc gttgtcgttg gcttcggtcg ccatggtcac ggtcctcatg
    18661 cggtctctcc gaggccaggt caccgcgacg gcgttatcca aggccgactc gctcgcccgg
    18721 gagttggagt cggggatcct cttggaggac ctcgtgttgg gcgatccgaa cgatgacgag
    18781 gaattcgttc agatcgtgga tgcgggaggc gaagtcctgg ggtcctcgcc caacgtgtcg
    18841 ggctggggac cgatcgaact ggtccgtccc gggacgacgc ggacgatccc cgcgcggagt
    18901 ccgccggacg tggagccgtt ggatgacccg ttcctcctcg tcgccagtga cgtcgatgga
    18961 gcggggggta tcgagtcggt gatcgtcgca cggaacctcg aaaccgtcat cgaaggccgc
    19021 tccgcgctca cgatcgcgct cgtcatcatc atgccgctcc tcttgctcgt cgtcggactg
    19081 gtcacctggc tcgtgacggc gagggccctg tcccccgtcg acgcgatcag ggccgaggtg
    19141 gacgcgatct cgactcgcga cctccatcgg agggttcccg atcctccggg cgatgatgag
    19201 atctcccggc tcgcgggcac gatgaaccgg atgctcgagc ggctggaggc cggccagcac
    19261 cgggcgcgtc gcttcgtctc ggacgcttcc cacgagctcc gctcgcctgt cgccgcgatc
    19321 cgccagttcg cggaagtcgc gctggcccat ccggagcgga cggctgtcgg cgaattggcg
    19381 gaggtcgtgc tcgaggagga tgcgcgcctc cagcggctcg tcgaggacct cctcttgctc
    19441 acgaggatcg acgagggaac gatccctcac ccgacgggag ccgtcgacct cgacgatctg
    19501 ctcttggagg acgctgtccg gctccgcgcg accaccgacc tgaaggtcga cgtccgctcc
    19561 gtctccgcag gacgggtcat cggagacgcc gaccaactcg aacggctcat ccggaacctg
    19621 accgacaatg cctcccgaca cgctcgaggc atggtccgcc tctctctcca ggaggtcggc
    19681 gggcgcacta tcctctgcgt cgacgacgac gggatcggcg tcgatccggc cgagcgagag
    19741 cacatcttcc aacggttcgt ccgtctcgat gacgcgcgag atagggattc cggggggagc
    19801 ggactcggat tggcgatcgt ccgagaggtc gcgacgttcc acggcggttc ggttcggatc
    19861 ctcgatagcg acctcggagg catccggttc gaggtcgatc tgccggcgca accggactga
    19921 gtgggcattc agcggacgtt cagccgagtg cggatagcct cgataggtat gcgacagctc
    19981 atgtcggacg cctcgaccca gagatctcga tggatgctcc ccgaagggat caccgggctg
    20041 acctcgcagc agatcatccg gctgctcttc tcggcaccca cggcggaggc ggcgcaacgc
    20101 gcaacgtcct ccttccggag cacctccccg atcgccttcg cggttccggc cgacacggaa
    20161 ctccgctgcc ggctgttcct cgagcgactg cgctggcccc acggcgtgta ctgcccgcgc
    20221 tgcgaggaca gccggaggat ctgccggatc gaggagagag cccagttcga gtgcggcagc
    20281 tgcggatacc agttcagcgt tcgggccggt acgatcctgc atgcctccca cctcccgctc
    20341 tggaagtggc tcttggcggt ctacctgatg gcctcggcgg acgacatcac gtcgtatcgg
    20401 ctcgaacgag ccctcgggat ctcctacaag accgcctcgc acctcactca cacgatccga
    20461 gcgctcatgg gtgatctttc gaacggggtc gacgatgcgg cgaaggtgga tcggcactcc
    20521 gggcgtgtcc gcgggcgggt ggagcgcccc atcgattcgc tcccgaagca cgccgatcgt
    20581 ccaaccacca gcggggatcg cctccttctc gtgcatctgc tctcgaggct cgtcaacgcc
    20641 ggctccggcc cgtccgaccg catggtagcc agcgcgtagc ccgactcgga ggacagtacg
    20701 cctggaggtc gtttcaggaa gaagacgagt agctcgtcat ccgcgccggt ccatcgacga
    20761 tcaagccgat ccctcgcgcg tatatcttcg tccccgtgcc gccgtccagg ggactgccat
    20821 cctccatcgt cacggtgtcg tcgaacgacc cggcggggac gtccgtcgac tctccgaccg
    20881 cgacgaccct cgcctgatcc tcggcgacgc ccggcgcgat ctcctgctcg aaggcgactc
    20941 caacctcgag ggagggtggc atgaagatcc caggttgatt ctcaccctcg cccgccctcc
    21001 actcgccgga gtgtgacgtg acgtcccctc cgctgtcgaa gatgtcgacg tcttcgccga
    21061 agtaacagac tgtcccgtcc gctgcctggg cgaagaagtt gcgggagatc tcgacgacag
    21121 tcccatcttt ggactcgaat tcctccacca ccctggtctc gacaccggcg acggtctcgg
    21181 tttcgtccag tgaggtgatc cgaaccagga ggtgcgtcga tccttcctca ccctcgagca
    21241 ccacttggtg gccgacgggg agcgggaaga acggattgtc gatatcgcta gagaagtcgc
    21301 ctgatgcggg atcacacgcg gcgctgctcg ccaggttctc gaagcccgag aaacgagcgc
    21361 agccccccac cggaaggagg gcgatacaca cagcgataac gcgtgcgtgt gtccgcgagc
    21421 tcatccctct gaccctagcc aaccgaggct gaatcgcggc tgaaatcgtc gccgcttagc
    21481 tccggacttc gagtggagcc gtcaagccgt agacgttccc gcgatcctgc atcgcgaagg
    21541 cctcagagtg cagctcatcc atctcatcag cctcgagctg tcggacctcg gtgaggacca
    21601 ggacctcgcg ctccctttcc ttcgctcctc gccatccgac acgaacgtag ccaacgtcgt
    21661 gggcatagaa cttcaattgg tacgcccctg gcttcaaggg ctcgaattcc tcggttacga
    21721 gcacgtcgtc atagcagccc gcaggcacac atgtgcgctg ccccgtcttg aagactcgac
    21781 cgaagtcgtc ccaatagtac ggaggtggtg cgtatccctg cgagtactcc ggatcgcctt
    21841 cctgatgctc cgcgggcatc gcgatgccgg ccgatgcacc gtccacatac cctacgagcc
    21901 agggggcggc gccctcgaac tgcttgccgt cgtacagttc cgcgtactgg ccgaggtgcc
    21961 agacgtttcc gtccagatcc tgcgcgaaga agatgagctc cttctcgacg agggcgccct
    22021 cctcgaagtc tcggtcccat ccaacgaccg cacggactcc ggcgatctct ttcgtgagcc
    22081 cagttaccgt gaagacgaca ctgtgttcga tgcgatcgcc ttcgtcttcg atcgacgatc
    22141 cttcgtagaa gaactgggtt ccgggtacca gcggaaagta ggtgttgtcg acattggcgg
    22201 agtccggccc gaagttcgcg gggtcgaagc cgttcccgtc gatggcgaag ggaggatccc
    22261 ctgcgggtag tagggcgtcc ggctcaagcg aaggcgtcgt cgcgatggtg cccgtcccgt
    22321 tgtcctcagg tgggatcgtg ccctcacacg ccgccatcga ggcgatcagg cctccgacca
    22381 ggacgaggat gtggatcccc ctcgtccgat cgaagatcat ggagccatcg ctatcgggcc
    22441 aggtgctcct cgcttcccga agcggaggta cagcgccggc acgacgaaca ggttcagcag
    22501 ggtcgccgtg atgagcccac cgaagatgac gaccgccatc gggtgctcga tctcgttgcc
    22561 agggatctga cccgccagga tcagcggcac gagcacgagt ccggtcgtga gcacggtcat
    22621 caggatcggg accacccgct cgcgcgctcc ccggatgatg agctccggtc cgaaggcgac
    22681 accttcgaag cgctcgaggt gctggtagtg gctgatcagc atgatcccgt tcctcgcgac
    22741 gatcccgagg accgtgaaga agcccaccag tgagcccaag gagatgatcc ctccgccgag
    22801 gtaggcggcg atcacgccac cgatgagggc gaccgggagc gtcacgaagg agagggtcgc
    22861 gagccgccag ctggcgaagg aggcctgcag gagcaggaag acaccgaggg ccgcgatgat
    22921 cgcgaacaat cgaagacgcc cggatgccgc ctgacgctcc gcgtactcgc cgagcatctc
    22981 cgcgtggtac ccgagcggcc actcgacgcc ttcgagacgt tgttcgaggt cgccgatcac
    23041 cgaaccgagg tctcggtctt gcacgttcgc gctcacgtcg atgctccgga acagcccttc
    23101 gtggtggatg tcgttcgggg tcggctggat ctcgatcgcc gcgacgtcct tgagccggac
    23161 gtagtctccg ctcggcgtgt cgatcagcag gttctcgatg tcggtgaggt tctcccgcgt
    23221 ctccggtgtg ctccacacgt tcacgtcgta ggtctggccg ttgcggaaga ggtcgccgac
    23281 ctcctcaccc atgatcaggg tcgaagcgga gcgccggacg tcgccgggct tcaacccgta
    23341 ctggcgcgcg gtctccagat cgacctcgat ctggatctgc gggatgtctt cgacggtgtc
    23401 cacgaaggga tccaccacgc ccgggatccc tccgaggagc tcctcgacct cctgcgcctt
    23461 ctcctggagc acggcgagat cgtcgccgaa gatccggatc acgatcgcgt cgctcgatcc
    23521 ggtgaggacc tcgcggatcc gctccttcag atacgtctgc acgtctcgga agatgcccgg
    23581 ataggccgcg accgtgtcct ggatcttctt caaggtggtg tcgtagtcga cggacgggtc
    23641 cacgctcacc cagttctcgc cgaagtagat gccgtagggc tcgtcggagt tgagcgcctg
    23701 gccgatgtgc gatccggcgt tgttcactcc cgggatctcg aggagttcgc ggttgacgag
    23761 catcgtcgtg cgaacctcct ccggacgcga cacgccgggc ttgccgagcc agtgcatcag
    23821 gaagtcccgt tccttgaacg aaggcaacag gtcctgcccg agcaggggca cgagcccgat
    23881 ccccagcatc gccgtcacac ccaccgcgac gtacgagcgg cgcggccgat agatgatctt
    23941 cgcgaggagg tactcgtatc cgcgctggag cggcgggacg agcggcgaga cgcgatgctg
    24001 cagggacttg ccgctccgga agaagatcag gctcatcgcc ggcgtgacgg tgagggcgat
    24061 caccatcgac acgaggaggg cgagcgcgta ggcgccggcg agcggccgga agaaggcacc
    24121 cgagaggccc tccagcgcga agatcggcac gaccgcgacc acctcgatca gggtggcgta
    24181 gacgatcgcg cttcgcacct cgagcgacgc ttcgaggatc acccgcgccg tggacttggt
    24241 gccgccctcg gctcgatgct cccggagccg acgcacgacg ttctcgatat cgatgatcgc
    24301 gtcgtcgacg atgtcgccga gcgcgatcac cagcccggcc agaaccatcg tgttgatcgt
    24361 cacgtccatc catgcgagca cgagtcccgc gccgatcagt gagagcggga tcgccgtcac
    24421 gctgatcacg gccgtccgcc actcgtagag gaacgctccg agcatcagga tcatcaggag
    24481 tgccccgatg atcatcgcct cgctgaggtt gtcgatcgcg ttctcgatga acgtcgcggg
    24541 acggaagatc gtcggatcga gcgtgatctg gggaagtgcc ggccggaggt cggcgatcgc
    24601 cgcgtcgagg ccggtcgtga cgtccgccgt gttcgcccag ggcagcttct gcacgacgag
    24661 cagcaggccg ggacggtcgt tgatgacccc gtcgcccatc aacggcggtg tgtcgatcac
    24721 gacgtcggcg acgtcgccca ggcggatcac gtcgccgccg cgcttgacga tcgggacccg
    24781 ggagagctgc tcaccggtga ggatcgggat cgtgtggcgc acctgcaagc ggttggtagg
    24841 cgtgtcgagg aacccgccgg tgccgatgaa gcccccctcc gagaaccgca gcaagcccga
    24901 gtcgaccgca tccgaggtga cggtcatgac ctcttcgagg gtgagcccgt gcttgcggag
    24961 gagctcaggg tcgacctcca ccgagggcat cttgatccgc tcgccccaga tcaggacgtt
    25021 cgcgacaccc gggacgcgga gcagtttggc gcggaccttc cagtaggcgg tcatcgacag
    25081 atcgaccagg tcgtattcgt cggaggtcat cccgatcttc atcacgcggc tcgtcgccga
    25141 gaggggctgg atgatcaccg gcggggcggc ccaccgcggc agcgtgggcg agacggcggc
    25201 gatccgctcg gccaccagct ggcgagcggt cagcagatcc gctccccgct cgaagatcat
    25261 ctcgatctgc gacagctgct cgaccgactt cgagcggatc gtgtcgagct cgggaacgcc
    25321 gttgagcgcc tgctccagcg ggacggtgac caggttctcg atctcggttc ccgagagccc
    25381 gagggcgagg gtctggatct cgacccgcgg gggagcgaac tccgggaaca catccaccgg
    25441 catgtgccgc agttgttgga cgccgaagaa catcagggcc gcggcagctg ccgcgatgat
    25501 gtatcggaac ctcaggctcg agcctacgat ccagcgaagc atcgcttcgt ccgtcctttc
    25561 gcgctcatgg tctcgtcaga tcccgaactc ggagccgatc aactcaggca cgccgaccga
    25621 cgcgacctcg gtgcccggcg ccggcccatc cgacaggtag gccaaaccgt cgtcgtaccg
    25681 tttgacgacg acctgggctc ggacgaagac gagagtctcc gggttcgtgt agacccactc
    25741 gtccccgttc acgtccaccc agatcgcaga ctccggcacc accgtccgac cgccacggcc
    25801 cgtcgtcggc accgtatcga gcccgatccg ccgggccgcg tcctcc
//
LOCUS       DASUYD010000139         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_734404, whole genome shotgun sequence.
ACCESSION   DASUYD010000139 DASUYD010000000
VERSION     DASUYD010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734404"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VF195_07595"
     CDS             complement(<1..661)
                     /locus_tag="VF195_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580718.1"
                     /translation="MESGLAEVDGGAIAWESAGEGPDVVFLHPGLWDWRVWDHQFGVF
                     SKIYHVVRYDLRGYGRSSRPEPGKPYSHVDDLAAVLDAAGVDAAALVGNSMGGRVAID
                     FALTHPKRVDAMVLAATNVGGFEETEEEAKAYADLDREIEQAVEAGDAERAMGLELSV
                     WAAALGTEDPAGARIRRIAFDNLHVITMDESGAAGLDDAFHRLPEIHVPALVLPADHD
                     PP"
     gene            complement(780..1274)
                     /locus_tag="VF195_07600"
     CDS             complement(780..1274)
                     /locus_tag="VF195_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014163987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6580719.1"
                     /translation="MERIWSPWRMAYIQAAKEQGKDGGCIFCDLPADGDDVRTMILAR
                     GELAFVIVNSYPYNPGHLMVAPFRHVGAFTSLEAAELADVDALVARSIRALEQEMEPH
                     GYNLGMNLGRVAGAGIPDHVHWHLVPRWNGDTNFMPVVGQTRVLPELLEETYARLRPR
                     FEGA"
     gene            complement(1298..>2110)
                     /locus_tag="VF195_07605"
     CDS             complement(1298..>2110)
                     /locus_tag="VF195_07605"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6580720.1"
                     /translation="RTRGFTQDDSHIVCREDQLVDEILGVFDLTLEIHRTFGFTDPVV
                     TLSTLPGETIVTPEMAERATAALRAALERSGLAFEVAEGEGSFYGPKVDFHFRDAIGR
                     LWQLTTVQCDFGLPERFEMEYVGEDNQRHRPVMIHRAILGSIERFSAVLIEHYGGAFP
                     LWLSPEQVRLVPVADRHVEHALELASLLRALGLRSEVDRSGETVGKKVRAAQLAKAPY
                     VLVIGDKEIESGELTVRDRDGVETKGVPFDDFAAALVDEAASKRLTQSRFGG"
BASE COUNT          331 a          762 c          684 g          333 t
ORIGIN      
        1 cgggcggatc gtgatcggcc ggcaggacga gagcgggtac gtggatctcg gggaggcgat
       61 ggaacgcatc gtcgagcccg gccgcaccgc tctcgtccat cgtgatcacg tgcaggttgt
      121 cgaacgcgat ccggcggatg cgcgctccgg cgggatcctc ggtgccgagg gccgcggccc
      181 agacagagag ctcgagtccc atcgctcgct cggcgtcgcc ggcctccacc gcctgctcga
      241 tctcccggtc gagatcggcg tacgccttcg cttcctcctc ggtctcctcg aacccgccga
      301 cgttcgtggc cgctagaacc atcgcgtcga cgcgtttggg atgcgtgagc gcgaaatcga
      361 tcgcgacacg tccgcccatc gagttcccaa ccaatgccgc agcgtcgaca ccggccgcat
      421 ccaggacggc ggcaaggtcg tcgacgtgcg agtagggctt ccccggctcc ggtcggctcg
      481 atcgaccgta cccgcgtagg tcgtacctca cgacgtggta gatcttcgag aacacgccga
      541 actggtggtc ccagacgcgc cagtcccaca ggccgggatg caggaagacc acgtccggcc
      601 cctctcccgc cgactcccat gcgatcgccc cgccgtcgac ctccgcgagg ccgctctcca
      661 tgcgtgtcat ggctcgtcgt tcactgcgag cgccaggaat tcaccgcggg cgggttgacg
      721 cgttcgggct tggagatctt cgcggggtcc cctccaccag atccgcgaac ccttcgctct
      781 catgcaccct caaagcgtgg ccggagccgc gcgtacgttt cctcgagcag ctccggcagc
      841 acgcgggtct gcccgacgac cggcatgaag ttcgtgtcgc cgttccagcg aggtacgagg
      901 tgccagtgca cgtgatcggg gatgccggcg ccggcgacgc ggccgaggtt catcccgagg
      961 ttgtacccgt gcggctccat ctcctgctcg agcgcacgga tcgaacgcgc caccagcgcg
     1021 tcgacgtccg cgagctcggc ggcctcgagc gaggtgaacg caccgacgtg ccggaagggt
     1081 gcgaccatca ggtgcccggg gttgtacggg tacgagttca cgatcacgaa cgcgagttcg
     1141 ccgcgggcaa ggatcatcgt tcgcacgtcg tcaccatcgg ccggtaggtc gcagaagatg
     1201 caacccccgt ccttgccctg ctccttggcg gcctggatgt acgccatccg ccacggcgac
     1261 cagatccgct ccacgcgggt cccccggcgt cctcgcgtca gccgccgaac cgcgactggg
     1321 tcaggcgctt cgaggcggcc tcgtcgacca gtgccgcggc gaagtcgtcg aacggcacgc
     1381 ccttggtctc aacgccgtcg cgatctcgga ccgtgagctc cccggactcg atctccttgt
     1441 caccgatcac caggacgtac ggcgccttcg cgagctgcgc ggcccggacc ttcttgccca
     1501 ccgtctcgcc gctccggtcc acctcggacc gcagaccaag agctcggagc agcgacgcca
     1561 gctcgagcgc gtgctcgacg tgacggtcgg ccaccgggac caggcgcacc tgctcgggcg
     1621 agagccacag cgggaacgcg ccgccgtagt gctcgatcag cacggcgctg aagcgctcga
     1681 tcgatccgag gatggcacgg tggatcatca ccggacgatg ccgctgattg tcctcgccta
     1741 cgtactccat ctcgaagcgc tcgggcaggc cgaagtcaca ctgcacggtg gtgagctgcc
     1801 acagccggcc gatcgcgtcg cggaagtgga agtccacctt gggcccgtag aacgacccct
     1861 caccctcggc gacctcgaag gcgaggccgc tccgttcgag ggcagcccgc agggcagccg
     1921 tcgcccgctc cgccatctcc ggcgtcacga tcgtctcccc cggcagtgtc gagagcgtca
     1981 ccacgggatc ggtgaacccg aacgtccgat ggatctcgag cgtgaggtcg aacacgccga
     2041 ggatctcgtc gacgagctgg tcctcccggc agacgatgtg cgagtcgtcc tgcgtgaacc
     2101 cgcgcgtccg
//
LOCUS       DASUYD010000140         5158 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_957240, whole genome shotgun sequence.
ACCESSION   DASUYD010000140 DASUYD010000000
VERSION     DASUYD010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5158
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957240"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF195_07610"
     CDS             complement(<1..293)
                     /locus_tag="VF195_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580721.1"
                     /translation="MPRRRWTVVIPICAVLFLPGCRDLATEEEVGYEPASVEEIEGSD
                     LLRVVLKEDAARRIGLDTVPTTGRGGRTVVPESAIWVDVNGDEWVYTNPETLV"
     gene            complement(305..3505)
                     /locus_tag="VF195_07615"
     CDS             complement(305..3505)
                     /locus_tag="VF195_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX6580722.1"
                     /translation="MMRRIVASSLKFRRLVVALAAGLVVFGVVQLRHVPVDQLPEFGP
                     TMVEVRTEALGLSATEVEQLITVPLEQDLLNGVAFVEEIHSESLPGLSSVVMIFEPGT
                     PLLDARQVVQEKVAEAAVALPGVSAPPQMLQPYSSTSRVLTVRLASDSLTPIEISELA
                     KWNIAPRLLGVQGVANVSIWGFRDRQLQVLVDPERLRDADLTLQQVVRTTGNSLWVSP
                     LTFLEASTPGTAGFIDTPNQRFSIRHELPIKTADDLAEIPIEDDEGNAVIVDGATVQL
                     GDVTDLVEDHQPLIGDAIFANGEAGLMLVVEKFPDANTGEVTDGVEEALDSLRPGLSG
                     LEIDTSLFRPADYVETSARNVGVALAIGGLLLIAGLALLLFEWRSIVIATAAIISSFA
                     AAAAILIIRDVTLNVMIIAGLVMALGIVIDDAVNDVRQIASRIKGGEENGAGASVPAW
                     RSVLEASVAVRSSLLFGTFVAVAAVVPGFFLDRQAGAFLPPILISYLVVIGVSMLVAL
                     VVTSSLGLMLLPGRSDPRESPVLHWFRDRHGSLLTRAVRRPRWAYAAIAVALVIGVAM
                     FPFLDRSSNVRLRESDLLIRWDATPGTSLTAMNGLTADVVDRLGALEGVEQVGAHVGR
                     AIHSDQIVNINSGEIWLTLDDSTDHDASVASIEKILATYPDLSHRVTTYAEQRITDVL
                     EGRDRDVVVRVYGADRATLEAKADEIRGALSGIEGIERPQVEVAPTEPTIEVEVDLLR
                     AQQFGVKPGDVRRASAILLSGLAVGNLFEEQKVFDVVVWGTPDIRRSVEDVEQLLIDT
                     PTGGRVRLHEVADVSVVENPTVIRHEAVSNYIDVSADIAGGDADGVLEDVRSVVAAVE
                     FPLEHHAEIRGAAVNGVDSGATVLAVAITAALAIFLLLQAAFTSWRLAILVLATLPIA
                     LVGGLVAAGIGGGTIGLGAFAGLLAVLGMTARSGIQMVRHYQHLELEEGETFGDELVL
                     RGTGERLQQSVVPVIVTILALVPIIVAGDVAGLEIAQPMAIVMIGGLLTSALLTLFVL
                     PPLYRMHGFVTERDTVAEDLVVLPEPVLDIEPVAGS"
     gene            complement(3671..>5158)
                     /locus_tag="VF195_07620"
     CDS             complement(3671..>5158)
                     /locus_tag="VF195_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017241863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX6580723.1"
                     /translation="ALGTAELLSAVFGPASSPIVAVGGAAVDATPEWLKSFAIRTFGE
                     HDKGVLLIGIGIVLGIAVAIAGAASWRRPRLAIGASVVLGAIGAAAAVTRPANDLSDA
                     VPSIAGAAAGILTSRLLMAAAGIPTGSAAEASPSPVMRSFDRRRFLRAGVAVAGIAAA
                     AGGLGRLLSSRATAAASRAAALIPAPGDIGPPPPTGADLGVPGVAPFLTPNATFYRVD
                     TALFVPAVDADSWTLRVHGMVDHEIALGFDDLLARPMIERDITIACVSNEVGGPYVGN
                     ARWIGAPLADLLREAGVHPDASQIVSRSSDGFTIGTPTSVVMDGRDAMLAVSMNGEPL
                     PLEHGFPVRMLVPGLYGYVSATKWLVDLELTTLHAYDAYWIERGWAKEAPVKTQSRID
                     TPGSGAEVAAGPVAVGGVAWAQHRGIDLVEVRVDDGAWDTAELGVEDTIDTWRQWVYR
                     WDATPGSHALAVRATDGEGETQTEETVPPFPDGATGHHTILVEVT"
BASE COUNT          855 a         1809 c         1772 g          722 t
ORIGIN      
        1 aagacgagag tctccgggtt cgtgtagacc cactcgtccc cgttcacgtc cacccagatc
       61 gcagactccg gcaccaccgt ccgaccgcca cggcccgtcg tcggcaccgt atcgagcccg
      121 atccgccggg ccgcgtcctc cttgaggacc acccgcaaca ggtccgaccc ctcgatctcc
      181 tccacggagg caggttcgta gcccacctcc tcctccgtgg cgaggtcccg gcagccgggg
      241 aggaagagga ccgcgcagat cgggatcacg accgtccatc gacgacgagg catcgttcct
      301 cccctcagct cccggccact ggttcgatgt cgagcaccgg ctccggcaag accacgaggt
      361 cttccgcgac ggtgtcgcgc tcggtgacga acccgtgcat ccggtacagc ggcggcaaca
      421 cgaacagggt gaggagcgcg gaggtcagga gcccgccgat catcacgatc gccatcggct
      481 gtgcgatctc gaggccggcg acgtcgccgg ccacgatgat gggcaccagc gcgaggatcg
      541 tgacgatgac cggcacgacc gactgttgca ggcgctcccc ggtgccgcgg agcaccagct
      601 catcgccgaa cgtctcgccc tcctcgagct cgaggtgctg gtagtgcctc accatctgga
      661 tgccgctgcg ggcggtcatc cccaggacgg cgaggagacc cgcgaaggcg ccgaggccga
      721 tggtccctcc accgatcccg gcggccacga gcccgccgac gagcgcgatg ggaagcgtcg
      781 cgaggaccag gatcgcgagc cgccagctcg tgaacgcggc ctggaggagg aggaagatcg
      841 cgagggcagc ggtgatcgcg acggccagga ccgtggcccc tgagtccacc ccgttcacgg
      901 cggcgcccct gatctccgcg tggtgctcga gcgggaactc cacagccgcg accacgctcc
      961 ggacgtcttc cagcacgccg tcggcgtcac cgcccgcgat gtcggcactg acgtcgatgt
     1021 agttggatac ggcctcgtgt cggatcacgg tcgggttctc gacgacgctg acgtcggcaa
     1081 cctcatggag gcggacccgt ccaccggtcg gtgtatcgat cagcagctgc tcgacgtcct
     1141 cgaccgaccg gcggatgtcg ggcgttcccc acacgaccac gtcgaacacc ttctgctcct
     1201 cgaacaggtt gcccacggcg agcccggaga gaaggatcgc cgacgctcgg cggacatctc
     1261 cgggcttcac gccgaactgc tgcgcgcgca ggagatcgac ctcgacctcg atggtcggct
     1321 cggtgggagc cacctcgacc tgtggtcgct cgatgccttc gatccccgac aacgctccgc
     1381 ggatctcgtc ggccttcgcc tcgagggtgg cccggtccgc gccgtacacg cgcacgacga
     1441 catcccgatc gcggccttcg agcacatcgg tgatccgctg ctccgcgtac gtggtcacgc
     1501 ggtgcgagag atccgggtag gtggcgagga tcttctcgat cgacgcgacc gaggcatcgt
     1561 gatccgtcga gtcatcgagg gtgagccaga tctccccgct gttgatgttc acgatctggt
     1621 cggagtggat cgcgcggccg acgtgggcgc ccacctgctc gacaccctcc agcgcgccca
     1681 gcctgtcgac gacgtccgcg gtcaacccgt tcatcgccgt caacgacgtg cccggagtcg
     1741 cgtcccaccg gatcaggagg tcgctctcgc gaagccgaac gttcgacgag cgatccagga
     1801 acgggaacat cgcgacgccg atgacgagcg ccaccgcgat cgcggcgtag gcccatcggg
     1861 gtctgcggac ggcgcgagtg agcagcgacc cgtgccgatc ccggaaccag tggagcaccg
     1921 gggactcgcg cgggtcggaa cggccgggca gcaacatcaa gccgaggctg gacgtgacga
     1981 cgagcgcgac cagcatcgag acaccgatca cgaccagata cgagatcagg atggggggca
     2041 ggaacgctcc cgcctgcctg tcgaggaaga atccgggcac gacggcggcg accgcgacga
     2101 aggtcccgaa gaggagggag ctccgcacgg cgacggaggc ctcgaggacc gatcgccagg
     2161 ccggcacgct ggcaccggct ccgttctcct cgccgccctt gatccgcgaa gcgatctgac
     2221 ggacgtcgtt gacggcgtcg tcgatcacga tgcccagcgc catcaccaag ccggcgatga
     2281 tcatcacgtt cagcgtgacg tcacggatga tgaggatcgc ggcggcggcc gcgaacgagg
     2341 agatgatcgc ggcggtggcg atcacgatcg agcgccactc gaacaggagt agggccagcc
     2401 ccgcgatcag gaggaggccg ccgatcgcga gcgcgacgcc gacgttccga gcggatgttt
     2461 cgacgtaatc agccgggcgg aagagggagg tgtcgatctc cagcccggac agtcccggtc
     2521 ggagcgagtc cagcgcctcc tccacgccgt ccgtgacctc acccgtgttc gcgtcgggga
     2581 acttctccac gacgagcatc aaccctgcct cgccgttggc gaagatcgca tcgccgatca
     2641 gtggctggtg gtcctcgacc aggtccgtca cgtcgccgag ctggaccgtg gccccgtcca
     2701 cgatgaccgc gttgccctcg tcgtcctcga tcgggatctc cgcgagatcg tccgccgtct
     2761 tgatcggcag ctcgtgacgg atcgagaacc gctggttcgg cgtgtcgatg aaccccgcgg
     2821 tcccgggcgt ggaggcctcc aggaacgtga gtggggagac ccagagggag ttccccgtcg
     2881 tccgcacgac ctgctggagg gtgaggtccg catcccgcag gcgctccggg tcgaccagca
     2941 cctgcagctg ccggtcgcgg aacccccaga tcgagacgtt ggccacgccc tggaccccga
     3001 gcaggcgcgg ggcgatgttc cacttggcga gctccgagat ctcgatcggc gtcagggagt
     3061 cggaggccag ccggacggtg aggacacggc tcgtggagga atacggctgc agcatctgcg
     3121 ggggtgcgga gacaccgggg agggcgacgg cggcctcggc gaccttctcc tgcaccacct
     3181 gtcgcgcatc gaggaggggc gtcccgggtt cgaagatcat gacgaccgag gagagcccgg
     3241 gcagggattc cgagtggatc tcctcgacga acgcgacgcc gttcagcagg tcctgctcga
     3301 gcggcacggt gatcagctgt tcgacctcgg tcgccgagag accgagcgcc tccgtccgga
     3361 cctccaccat cgtggggccg aactcaggca gttgatcgac cgggacatgg cgcagctgaa
     3421 caaccccgaa gacgaccagg ccggccgcga gcgcgaccac gagacggcgg aacttcaggc
     3481 tcgatgcaac gatcctgcgc atcatcgacg ggacccctcc tctcgcggtg ggttcgcggt
     3541 ggaccgttcc gggccgctcg cccgcccccc gttgcgcgac aggtcgaagg atgggccacc
     3601 tgtagcaact ccacatgcgt cgtctgacgt accgccgata cccagttggg ctacatcatc
     3661 tggcgagagg tcacgtcacc tcgacgagga tcgtgtggtg accggtcgcg ccgtccggga
     3721 acggggggac ggtctcctcc gtttgcgtct cgccctctcc gtcggtggcg cgcaccgcca
     3781 gcgcatgcga accgggcgtc gcgtcccatc gatagaccca ctgccgccac gtgtcgatcg
     3841 tgtcttccac gccgagctcg gccgtatccc aagctccgtc gtcgacccgc acctcgacga
     3901 ggtcgatccc gcggtgctgc gcccatgcca cgccccccac cgcgacgggg cccgcggcga
     3961 cctctgctcc ggagcccggc gtatcgatcc gcgactgggt cttgacgggc gcttccttgg
     4021 cccacccccg ctcgatccag taggcgtcgt aggcgtggag cgtcgtgagc tcgaggtcga
     4081 cgagccactt cgtcgcggag acgtagccgt agagccccgg gacgagcatc cgcacgggga
     4141 acccgtgctc cagcggcagg ggctcaccgt tcatcgagac cgcgagcatc gcgtcccgtc
     4201 cgtccatcac gacggaggtc ggcgtgccga tcgtgaatcc gtcggaggac cgtgagacga
     4261 tctgcgaggc atcgggatgc acgccggcct ctcgaaggag gtcggcgagg ggcgccccga
     4321 tccaacgcgc gttccccacg tagggcccgc cgacctcgtt cgagacgcag gcgatcgtga
     4381 tgtcgcgctc gatcatcggt cgcgcgagga gatcgtcgaa gccgagggcg atctcgtgat
     4441 cgaccatccc gtggacgcga agggtccacg agtcggcgtc gaccgcgggc acgaacaacg
     4501 ccgtgtccac gcgatagaag gtcgcgttcg gtgtgaggaa cggcgccacg cccggcacgc
     4561 cgaggtccgc gcccgttgga gggggcggac cgatgtcccc cggagcgggg atcagcgcag
     4621 ccgctcgcga agcagcggcg gtcgcgcggc tcgacaggag ccggccgaga ccgcccgccg
     4681 ccgccgcgat cccggcgacc gcgacgcccg cacggaggaa ccgtcggcgg tcgaagctcc
     4741 gcatcaccgg cgacggagat gcctccgcgg cgctccccgt cgggatcccg gccgcggcca
     4801 tgagcaggcg ggacgtgagg atgcccgcgg cggctcccgc gatcgacggg accgcgtcgc
     4861 tgaggtcgtt ggcgggccgg gtgacggcgg cggccgcgcc gatcgccccg aggaccaccg
     4921 aagcgccgat cgcgaggcgc ggccgccgcc atgacgcggc accggcgatc gcgaccgcga
     4981 tgccgagcac gatgccgatc ccgatcagga ggactccctt gtcgtgctcg ccgaacgtcc
     5041 ggatcgcgaa ggacttgagc cactcgggcg tcgcgtcgac cgcggcgcca cccacggcga
     5101 cgatcggaga ggaggccggg ccgaagaccg cggagaggag ctccgccgtt ccgagggc
//
LOCUS       DASUYD010000141         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_734849, whole genome shotgun sequence.
ACCESSION   DASUYD010000141 DASUYD010000000
VERSION     DASUYD010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_734849"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VF195_07625"
     CDS             <1..1063
                     /locus_tag="VF195_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580724.1"
                     /translation="DSIMANLKRTLFGYSSASVRSTLTERDASLDRASENASAAEQRA
                     ERLSSELSETQGRLAELEEQLDTAQKLSLTLNADLERAVAERESMKAEILSLRSELEE
                     VHTELRDAQEAILGQEEQLRAAQAGSASLEERLLQQAERLEQAEASESHGSLQRALEE
                     SARYKATVSAQQERLTELEVLVEGYRELVETQGAAAPPSAEPGDAQEPPTGPSTASEL
                     AAVIEVAEQAVASIMDSTRVRADEELRAVDGERERIRRDVETMTAWRDRAEPMIVSLQ
                     AAMQEIQSQGEEISLRVNEVLRPVSTAVTALGAQLASLDDLDRAPLEVSTEAAAPSEP
                     DHVTQLPEEHPASRDSSDW"
     gene            complement(1124..1513)
                     /locus_tag="VF195_07630"
     CDS             complement(1124..1513)
                     /locus_tag="VF195_07630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580725.1"
                     /translation="MTSRDAPFVESVSTMLKLAQSDPEKARVALVARGDQVLASLRDS
                     MLPEAKESQRIVALIDLYVQATFEMLVEAARSDDRIEFGEAEIKAWAVAEALDRGAEG
                     ALKRDHDALTASMRAAEAILGLDFMST"
     gene            complement(1815..2498)
                     /locus_tag="VF195_07635"
     CDS             complement(1815..2498)
                     /locus_tag="VF195_07635"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEX6580726.1"
                     /translation="MDPADLDEVPEPDNKTQGLNLSPRLNFVPPPELPTPETASIQAR
                     ARALGIDALVWLAAFLPLTILYGGISSSNGLFRIRIGGPPLLMATVLWLAYMTWMEAK
                     HGASIGKRARGLHVVMEDGEPVNPEAALTRNLMRFLDAFPYVVPYLVGAYAISNSPKM
                     QRFGDRVAETMVVVSPPAPDPLHAGLPPLGSNPPRPKTRPTRRLVVVAVTLLVVGGAA
                     AFFLMRGTS"
     gene            2820..>3221
                     /locus_tag="VF195_07640"
     CDS             2820..>3221
                     /locus_tag="VF195_07640"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6580727.1"
                     /translation="MFRRRHSEDGDGSVQPGEPGIKETLGPEMTVVGRGAQVEGTLVS
                     TESIRIDGQVKGKIAARCDVILSSHSDVEADIRAQNVVSGGTLKGTITARTRTEVAQG
                     GRVEGKIRSKELVVSEGGLFSGQADIDLDDAP"
BASE COUNT          568 a         1070 c         1118 g          465 t
ORIGIN      
        1 ggactccatc atggcgaatc tgaagcgcac gctcttcgga tacagctcgg cgagcgttcg
       61 gtcgacgctc accgagcgag acgcctcgct cgaccgggct tcggagaacg cgagtgcggc
      121 ggagcagcgg gccgaacgcc tttcctccga gctctccgag acgcagggcc ggctggcgga
      181 gctcgaggag cagctcgata ccgctcagaa gctctccttg acgctgaacg cggatctcga
      241 gcgagcggtc gctgagcgcg agtcgatgaa ggccgagatc ctctccctcc gcagtgaact
      301 cgaggaggtg cacacggagc tccgcgacgc ccaggaagcc atcctcgggc aggaggagca
      361 gctccgtgcg gcccaggcgg gatccgcgag tctcgaggag cgcctgctgc agcaggccga
      421 acgcttggag caggccgagg cctcggagag ccatgggtcg ctgcaacggg ctctcgagga
      481 atcggcgagg tacaaggcga cggtgagtgc ccagcaggag cgcctgaccg agctcgaagt
      541 gctcgtcgaa ggctatcggg agctggtcga gacgcagggc gccgccgcgc cgccatcggc
      601 cgaaccgggt gacgcgcaag agccgcccac cggcccctcg accgcgagcg agctggccgc
      661 cgtgatcgag gtcgccgaac aggcggtcgc gagcatcatg gactccaccc gggtccgggc
      721 cgatgaggaa ctccgcgccg tcgacgggga gagagagcgg atcaggcggg acgtcgaaac
      781 gatgacggcg tggcgagatc gagccgagcc gatgatcgtc tcgctgcagg ccgccatgca
      841 ggagatacag agccaagggg aggagatcag tcttcgcgtc aacgaggtcc tccgcccggt
      901 ctcgaccgcc gtgacggccc tgggcgcgca gctcgcgtcg ctggacgacc tcgatcgagc
      961 acctttggag gtctcgacgg aggcagcggc tccatcggag cccgaccacg tgacgcagct
     1021 ccccgaggag caccccgcga gccgcgactc ctccgactgg tagcccatcc ggagtcgggc
     1081 ccagagcgca tttccggcgc tgcccctcag gagcgtcgga tagctaggtc gacatgaagt
     1141 cgagacccag gatcgcctcg gccgcgcgca tcgaggccgt cagggcgtca tgatcgcgtt
     1201 tcagggcccc ttcggctccc cggtccagcg cctcggccac cgcccaggcc ttgatctcgg
     1261 cctcgccgaa ctcgatccgg tcgtccgaac gtgccgcctc gaccagcatc tcgaaggtcg
     1321 cctgcacgta gaggtcgata agagccacga tccgctgcga ttccttggcc tcgggcagca
     1381 tcgaatcgcg caaggacgcg agtacctgat ctccgcgggc caccagcgcc actcgggcct
     1441 tctcggggtc cgactgcgcc aacttcaaca tggtcgagac cgactccacg aacggggcgt
     1501 cccggctggt catgcctcct cctcactgcc cggcccacca gctctgtcgg ctggacctac
     1561 cgcatatcct ccaaccgggg cgagaccccg acaatcggcc gatggtccat attccccccg
     1621 tcggggggac cggctacatg cggcggggta ggtctttcga cggggtcggg ggtgcccgaa
     1681 ggtcgcccta gaacggggtc aagcccagtg gcgcaggcct ccggccgttt tcgagcaccc
     1741 aacgcgaagg ccccggggtc cctccgaggc ctctgcacgt ctcgtgatca ggaccgtgga
     1801 cgcttcgtga tcgcttagct cgtaccccgc atcaggaaga aggcggccgc cccgcccacc
     1861 acgagcaggg tcacggccac cactacgagc cggcgcgtgg gacgggtctt gggccgcggt
     1921 gggttcgatc cgagcggagg gaggcctgca tgcaagggat cgggcgccgg cggggagacc
     1981 acgaccatgg tctcggcgac ccggtccccg aatcgctgca tcttcggtga attcgagatg
     2041 gcgtaggccc cgacgagata ggggaccacg tagggaaacg cgtccaggaa cctcatcagg
     2101 ttgcgggtga gcgcggcctc ggggttcacc ggctccccgt cttccatgac gacgtgcagc
     2161 cccctggcac gcttgccgat cgaagctccg tgcttggctt ccatccaggt catgtacgcc
     2221 agccacagga ccgtcgccat gagcaagggg gggccgccga tcctgatccg gaagagccca
     2281 ttcgacgagc tgatgcctcc atagaggatc gtcagcggga ggaaggccgc aagccagacc
     2341 agtgcatcga tgcccagggc tctcgcgcgg gcttggatgc tcgcggtttc gggggttggg
     2401 agctcgggcg gggggacgaa gttcaggcga ggcgagaggt tgagcccttg cgtcttgttg
     2461 tccggctccg gcacctcatc cagatctgct gggtccatcc gcgcccctct cctcccgatc
     2521 acgcaggcag cagtaccgcc tggtagagca atcggaccaa gcgacccgaa cctttagacc
     2581 gtcgtgccgg gtcgggcgat ctcagcacac cccaggcgct tcgcccgaac gcaaacccgc
     2641 taaagcatgc gcgcctggtt gacgatgcca ccggagacga cgagccgtgc cgatgcggca
     2701 cgagatcgcg ggagagggtc aagggaccca gaacggttga tccggctccg tctcctaccc
     2761 gtgagcggtc gcggtcgggt cgccgcccgc gggaaccgca gacccgtcga ggaggcacga
     2821 tgttcaggcg acgacattcc gaggacggcg acggttcggt ccagccaggt gaacctggga
     2881 tcaaggagac gctcggcccg gagatgaccg tcgtgggtcg tggggctcag gtggagggga
     2941 cgctggtatc aaccgaatcg atccgcatcg acggccaggt caaggggaag atcgccgcac
     3001 gatgcgacgt gatcctctcg tctcacagcg acgttgaggc cgacatccgg gcccagaacg
     3061 tggtgtcggg cgggaccctg aagggcacga tcaccgcgcg gaccaggacc gaggtggccc
     3121 agggcggccg ggtggagggc aagatccggt ccaaggagct ggtggtgagc gaggggggcc
     3181 tgttctccgg ccaggcggac atcgacctcg acgacgcccc a
//
LOCUS       DASUYD010000142         6486 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_74100, whole genome shotgun sequence.
ACCESSION   DASUYD010000142 DASUYD010000000
VERSION     DASUYD010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74100"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VF195_07645"
     CDS             <1..1156
                     /locus_tag="VF195_07645"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6580728.1"
                     /translation="SASFLIVLSLPFWLQPHPEDDGRGIKQGLSGTTTLPDDIQTKQA
                     FLALVEYFPKAGANAPAQLAILGTPDDPEVQQGLETLTAEIADDPSLGAPAEPRVSED
                     GTVTLVEIPLTGEATDTQSEAAVLAIRELRGTYIPEAFGGSGVRVLVGGETAFITDFF
                     DVSSDYTPLIIVIVLGLSFLLLTVVFRSLVVPAKAIVMNLLSVGAAYGLIVLVFQKGG
                     PAIGKTIADAFGFQQTDAIEAWLPLFLFSILFGLSMDYHVFLLTRIREEYDKTKDNAE
                     AVTYGLRTTGGIITGAAIIMVAVFAGFAAGRLTALEEMGFGLAIAVLIDATIVRSILV
                     PSAMKLLGDRNWYLPRWLQWLPDLDVEGHVEQKPPVLVPEAEPVGVRDER"
     gene            1228..2163
                     /locus_tag="VF195_07650"
     CDS             1228..2163
                     /locus_tag="VF195_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6580729.1"
                     /translation="MEEPQGPELRGERDELRETLGRVGVWSFALESLTAAEEREAVAE
                     IESFGYGAIWFPESVESREVFSHAGWILASTERAIVVSGIANVWARDPTAMANGWRML
                     TDAYPERFVLGVGVSHAGSVARRGGTYERPYTAMRDYLDSMNRAPSSAPENDWTPRLV
                     LAALGPRMLELGAERAHGVHSYFVPVEHTAFARQRLGPGPVLAVEQTVVLESEPSEAR
                     RVARGFALDYLQSENYARNLRRMGWTGADVSGQGSDALIDAVIAWGDVDRVALRVRQH
                     LDAGADHVCVQVVAQDELDPCLQQLGELAPALLEL"
     gene            complement(2189..2881)
                     /locus_tag="VF195_07655"
     CDS             complement(2189..2881)
                     /locus_tag="VF195_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012899575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX6580730.1"
                     /translation="MSADERARDVVRALELVGDRSRLEGMARFGIDTTRAVGVTVTEL
                     RRFARDLGHDHELAVALWASGVHEARLLASLVDEPAMVSEAQMEAWVADLDSWDVCDG
                     VCGNLFDRTPFALDKAVEWSTREPEFEKRAAFALMASAAVHRKDLPDAEFASLLPVIR
                     AQAIDERNYVKKAVSWALRQIGKRSPRLNSQAVRTAEQIERIDSRAARWVARDALREL
                     QSDAVQARLRKS"
     gene            complement(2906..3178)
                     /locus_tag="VF195_07660"
     CDS             complement(2906..3178)
                     /locus_tag="VF195_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580731.1"
                     /translation="MAVFLLILLVLAAATGILGAVLKVTLVIVLSLVLSIVLLAWIGA
                     WYAKRRMRAFQRDFQLRADENRRRRQAYDIGSETRQDPARRLGEGS"
     gene            3358..4479
                     /locus_tag="VF195_07665"
     CDS             3358..4479
                     /locus_tag="VF195_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580732.1"
                     /translation="MAGGEVVLVDLVKRFADVTAVAGINLSMPSGEFFSLLGPSGCGK
                     TTTLRLIAGFERPDEGQILLDGIDMVQTPPHKRNVNTVFQNYALFPHLSVAENVAFGL
                     RYKEVSKQDAKKRVADSLELVRLQGLERRRPSQLSGGQQQRVALARALILNPAVLLLD
                     EPLGALDAKLRKALQIELKALQEEIGITFIYVTHDQEEALTMSDRLAVMSDGRVEQIG
                     TPSDVYEEPTTAYVADFLGVSNLMDATGGGAGPDGRGKVRLGEFELVAGHGDTDGRGD
                     IKIVIRPERVRLEETGARGENRVPGMVERVVYVGSIMQVIVHLAPGQTVQAWVQNQGE
                     GLPYGQGHPVSVHLPADALRVLVDNGAPIQQDLEDAAAG"
     gene            complement(4492..5415)
                     /locus_tag="VF195_07670"
     CDS             complement(4492..5415)
                     /locus_tag="VF195_07670"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580733.1"
                     /translation="MSDTTLAPERRLATAPARSTTGWLRDPWRKPRVLQAVTWGYLAW
                     SILPVVIAIIFSFNAGRSRSTWQGFSFRWWYQDAFDSVWNDPALRTAMFQTLRLAVLT
                     MLIAVPLGTAFAIGIDRWRGRPAATANFVMLFSFVMPEIILGVSLFLLFSNLLEGAVR
                     LGTTAQLLGLVTFQISYPFIIVRARLLSIGPEYEESAMDLGASPTQGLRRVLFPLLAP
                     AILASAALVFADSVDNFVTVRYLSGPASSEPLSVKIYSAARSSPTPAVNAAATIMLVT
                     TLTAIAVAWFLYRRITRSRGEAISSAGIADF"
     gene            complement(5412..6359)
                     /locus_tag="VF195_07675"
     CDS             complement(5412..6359)
                     /locus_tag="VF195_07675"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580734.1"
                     /translation="MASGIEVTPGRRRGRGVRPARSGVHYARWYWGSFTLPGTIWLAT
                     FFVLPFYVVLSVAFGTVDPLFRLPLPVYQPWWWSFATFQTTLEKFYAGDRIYFDPLLR
                     TLMYVAAASLICLVLGYAVAYYTARYAGRWKGLILVLLISPFWISYLMRIYAWQSLLQ
                     PDGYINDVGGLFGLSPVIWIGRPVTVVLGLVYGYIPFMILPLFGFLDRIDQHLLEAGR
                     DLGAGSFETFRRVTLPLSKPGILAGLVIVSLPMFGDYYTNNLLGSTNTSMYGNLIDSA
                     VGQAGQGPAAGSLVLILVAIVMIPMLYYLRSTRRTVESA"
BASE COUNT         1088 a         2145 c         2299 g          954 t
ORIGIN      
        1 ctcggccagc ttcctgatcg tcctgtcgct cccgttctgg ctccagcccc accccgagga
       61 cgacggtcgc gggatcaagc agggattgtc cgggaccacg acgctgccgg acgacatcca
      121 gaccaagcag gcgttcctcg ccctcgttga gtacttcccg aaggcgggcg caaacgcacc
      181 ggcgcagctc gcgatcctcg gcaccccgga cgacccggag gtgcagcagg ggctcgagac
      241 gctgaccgcc gagatcgccg atgacccgtc cctgggcgcg cccgcggagc cgcgcgtgag
      301 cgaggatggg accgtcacgc tcgtcgagat cccgctcacg ggcgaggcga cggacacgca
      361 gagcgaggcc gcggtgctgg cgatccgaga actccgcggg acgtacatcc cggaggcgtt
      421 cggcggctcg ggcgttcggg tgctggtggg cggcgagacc gcgttcatca ccgacttctt
      481 cgacgtctcg agcgactaca ccccgctcat catcgtgatc gtcctggggc tgtcgttcct
      541 gctgctcacg gtggtgttcc gatcgctcgt cgtgccggcc aaggcgatcg tgatgaactt
      601 gctctcggtc ggagcggcgt acgggctgat cgtcctcgtg ttccagaagg gcggcccggc
      661 gatcgggaag acgatcgccg acgcgttcgg gttccagcag accgacgcga tcgaggcgtg
      721 gctcccgctg ttcctgttct cgatcctgtt cgggctgtcg atggactacc acgtcttcct
      781 cctcacgcgg atccgtgagg agtacgacaa gaccaaggac aacgccgagg cggtcacgta
      841 cgggcttcgg acgaccggtg ggatcatcac cggcgcagcg atcatcatgg tcgccgtctt
      901 cgcaggattc gcggccggca ggctgacggc tttggaggag atgggattcg ggctcgcgat
      961 cgcggtcctg atcgacgcca cgatcgtgcg ctcgatcctg gtcccctcgg ccatgaagct
     1021 cctcggtgat cgcaactggt acctgccgag gtggttgcag tggctgcccg acctcgacgt
     1081 cgaggggcac gtcgagcaga agccaccggt cctggttccc gaagccgagc ccgtgggagt
     1141 cagggacgag cgctgatcac ggccgtcacc aggtgaggaa ggggcctccg tgcggaggcc
     1201 ccttccgcgt tgatagggtc cggcgccatg gaggaacccc agggccccga gctgcgtgga
     1261 gagcgcgacg aactgcgcga gacgttggga cgcgtcggcg tctggagctt cgcgctcgag
     1321 tcgttgaccg ccgccgagga gcgcgaggcc gtggccgaga tcgagtcgtt cggctacggg
     1381 gcgatctggt tccccgaatc cgtcgagagc cgcgaggtct tctcccacgc gggatggatc
     1441 ctcgcaagca ccgagcgcgc gatcgtggtg agcgggatcg ccaacgtctg ggcccgcgat
     1501 ccgaccgcga tggcgaacgg ttggcggatg ctcacggacg cgtacccgga gcgcttcgtg
     1561 ctgggggtcg gggtgagcca cgcgggttcg gtcgcgcgcc gtggcggcac ctacgagcgg
     1621 ccatacacgg cgatgcgtga ctacctcgac tcgatgaacc gcgcgccgtc ctcggctccc
     1681 gagaacgatt ggacgccgcg cttagtgctc gcggcgctcg ggccgaggat gctcgagctc
     1741 ggtgccgagc gcgcgcacgg cgtccattcg tacttcgtgc ccgtcgagca caccgcgttc
     1801 gcgaggcagc gcctcggtcc cggcccggtg ctcgccgtcg agcagaccgt ggtgctcgag
     1861 agcgagccct ccgaagcccg acgcgtggcg cggggattcg cgctcgacta cctgcagtcc
     1921 gagaactacg cgcggaacct gaggcgcatg gggtggacgg gcgcggacgt gtccgggcag
     1981 ggcagcgacg cactgatcga cgcggtgatc gcctggggcg acgtcgaccg cgtggcgctg
     2041 cgggttcggc agcacctgga cgcaggggcg gaccacgtgt gcgtccaggt cgtcgcccag
     2101 gacgagctcg atccgtgcct tcagcagctc ggcgagctcg cacccgcgtt gctcgagctg
     2161 taatcgagct cgcgactcgc gccggagctc aggacttccg gagacgggcc tgcaccgcgt
     2221 cgctctggag ctcacgcagg gcgtcgcgag cgacccaccg cgcggcccgc gagtcgatcc
     2281 gctcgatctg ctcggcggtt cggaccgcct gcgagttgag cctcgggctc cgcttgccga
     2341 tctgtcggag cgcccacgag accgccttct tcacgtagtt ccgctcgtcg atcgcctgcg
     2401 ctcggatcac gggcaacagc gacgcgaact cggcgtcggg caggtccttg cggtggacgg
     2461 cggccgacgc catcagcgcg aacgcggctc gcttctcgaa ctcgggctcc cgggtgctcc
     2521 actcgacggc cttgtccagg gcgaacggcg tccgatcgaa gaggttgccg cacaccccgt
     2581 cgcacacgtc ccacgaatcc agatcggcaa cccacgcctc catctgggcc tccgagacca
     2641 tcgcgggctc gtcgaccagc gacgcgagca ggcgcgcctc gtggacgccc gatgcccaga
     2701 gcgccacggc cagctcgtgg tcgtgcccga ggtctcgcgc gaaacgccgg agctcggtga
     2761 cggtgacgcc cacggcgcga gttgtgtcga tcccgaaccg cgccatcccc tcgagcctcg
     2821 agcggtcgcc caccagctcg agggctcgaa cgacgtcgcg tgcgcgctcg tccgccgaca
     2881 tcgccggaac gggttggcgt gtcgcctagc tgccctcgcc caggcgccgg gccgggtcct
     2941 gccgcgtttc ggagccgatg tcataggcct ggcggcggcg acggttctcg tcggcccgaa
     3001 gctggaagtc gcgttggaag gcgcgcatcc gccgcttcgc gtaccaggcg ccgatccagg
     3061 ccaagagcac gatcgacagg acgagggaca gcacgatcac cagcgtgacc ttgagcaccg
     3121 cgccgaggat gcccgtggcc gcggcgagca ccagcaggat cagtaggaac acagccatgc
     3181 caccatccta cgagcgtcgg cgccgaacgc gccgcccgtc ccagagaccc tcgagatccg
     3241 ggcaagcgcg ccgacgccga gccgtcgtgg accgatgggt ggctcgtacc cggcgcgtga
     3301 gcgattgcgg tcgacgaccc gaacggtgac gcggagcctc gggtatgctc cgcgaacatg
     3361 gccggtggag aggtcgtcct cgtcgatctc gtgaagcggt tcgccgacgt cacggcggtc
     3421 gcggggatca acctgtcgat gccgtccggc gagttcttct cgttgctcgg cccctccggc
     3481 tgcggcaaga cgaccacgct gcgcctcatc gccggtttcg agcgcccgga tgaaggccag
     3541 atcctgctgg acgggatcga catggtgcag acgcctcccc acaagcgcaa cgtcaacacg
     3601 gtcttccaga actatgcgct cttcccgcac ctctcggtcg ccgagaacgt cgcgttcggg
     3661 ttgcgctaca aggaggtctc gaaacaggac gcgaagaagc gtgtggcgga ctcgctggag
     3721 ctggtccggc tccagggcct cgaacgccgg cgaccctcgc agctctcggg tgggcagcag
     3781 cagcgtgtgg cgctggcgcg ggccttgatc ctgaacccgg ccgtgctcct gctcgacgag
     3841 ccgctggggg ccctcgacgc caagctccgc aaggcgctgc agatcgagct gaaggcgctg
     3901 caggaggaga tcggtatcac gttcatctac gtgacccacg accaggagga agcgctcacg
     3961 atgtccgacc ggctcgccgt gatgagcgac gggcgggtcg agcagatcgg aacgccctcg
     4021 gacgtgtacg aggagccgac gaccgcgtac gtcgcggact tcctgggcgt ctccaacctc
     4081 atggacgcga cgggcggcgg ggccggcccc gacgggcgag ggaaggtccg gctgggcgag
     4141 ttcgagctcg tggccgggca cggtgacacg gatgggcgcg gggacatcaa gatcgtgatc
     4201 cgacccgagc gcgtccggct cgaggagacg ggagcaaggg gggagaatcg ggtcccgggc
     4261 atggtcgagc gtgtcgtcta cgtcggctcg atcatgcagg tgatcgtgca cctcgcgccg
     4321 ggtcagactg tgcaggcgtg ggtgcagaac cagggcgagg gtctgcccta cgggcaggga
     4381 cacccggtgt ccgtccatct gccggccgac gcgcttcgcg tcctggtcga caacggcgcg
     4441 ccgattcagc aggacttgga ggacgcggcc gccgggtgac agcccggaga cctagaagtc
     4501 ggcgatcccg gccgacgaga tcgcctcgcc gcggctccgc gtgatccggc ggtagaggaa
     4561 ccaggccacg gcgatcgcgg tgagggtcgt gaccagcatg atggtcgcgg cggcgttgac
     4621 ggccggcgtt ggcgaggagc gggctgccga gtagatcttg acggacagcg gctccgacga
     4681 cgccggcccc gacaggtacc tgacggtcac gaagttgtcg accgagtcgg cgaagacgag
     4741 cgcggcgctc gccaggatcg cgggcgctag cagcgggaaa agcacccttc gcagcccctg
     4801 ggtgggggag gctccgagat ccatcgccga ttcctcgtat tcgggcccga tcgacaagag
     4861 ccgggcgcgg acgatgatga agggatagga gatctggaac gtcaccagcc cgagcagctg
     4921 cgccgtcgtc cccagccgga ccgcgccctc gagcaggttg gagaacagca ggaacagcga
     4981 gacgcccagg atgatctcgg gcatcacgaa cgagaagagc atgacgaaat tggccgtggc
     5041 cgccggcctc ccgcgccacc ggtcgatccc gatcgcgaac gccgtgccga gcggcacggc
     5101 gatcagcatc gtgagcaccg ccagacggag ggtctggaac atcgcggttc ggagcgcggg
     5161 gtcgttccag acggagtcga acgcgtcctg gtaccaccac cggaacgaga agccctgcca
     5221 ggtgctgcgg gacctgccgg cgttgaacga gaagatgatc gcgatcacga ccgggaggat
     5281 cgaccacgcg aggtagcccc acgtgaccgc ctgcaggacc ctcggcttgc gccacggatc
     5341 gcggagccat ccggtggtgc ttcgagcggg agccgtggcg aggcgtcgtt ccggcgccag
     5401 cgtcgtatcg ctcatgccga ctcgaccgtg cgccgtgtcg atcgcaggta gtagagcatc
     5461 gggatcatga cgatcgcgac gaggatcagc acgagcgatc ccgccgcagg cccctggccg
     5521 gcctggccca cggcggagtc gatcaggttg ccgtacatcg acgtgttcgt gctgccgagc
     5581 aaattgttcg tgtagtagtc cccgaacatc ggcagggaca cgatgacgag cccggccagg
     5641 atgccgggct tggacagcgg gagggtcacg cgccggaagg tctcgaagga tccggctccc
     5701 agatcacgcc cggcttccag caggtgttga tcgatccgat cgaggaagcc gaacaggggc
     5761 aggatcatga acgggatgta gccgtacacg agcccgagca ccacggtgac cggtctgccg
     5821 atccagatga cgggcgagag gccgaacaga ccgcccacgt cgttgatgta gccgtcgggc
     5881 tggaggaggc tctgccacgc gtagatcctc atcaggtagc tgatccagaa cggcgagatg
     5941 agtagcacga ggatcagacc cttccagcga ccggcgtatc gcgccgtgta gtaggcgacc
     6001 gcgtagccga ggaccaggca gatcaggctc gccgccgcga cgtacatcag ggtgcggagc
     6061 agcggatcga agtagatccg gtcgcccgcg tagaacttct ccaaggtggt ctggaaggtg
     6121 gcgaagctcc accaccatgg ctggtacacg gggagcggga gtcggaacaa gggatccacc
     6181 gtgccgaacg ccacggagag cacgacgtag aagggcagga cgaagaaggt cgccagccag
     6241 atggtccccg gaagggtgaa cgacccccaa taccaccggg cgtagtgaac cccggacctc
     6301 gccggccgca cgccccggcc gcggcgccgc ccgggtgtga cctcgatgcc gctagccacc
     6361 tgcctggatc tcgctccagg cgtccagcca gagcgcgtcg gcttcggcgg acaattcgcc
     6421 ctgcaggtag cccagcgtga agttctcctc gccgaggacg gcgagcgaga gcgactcggg
     6481 gacgac
//
LOCUS       DASUYD010000143         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_75837, whole genome shotgun sequence.
ACCESSION   DASUYD010000143 DASUYD010000000
VERSION     DASUYD010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75837"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1045
                     /locus_tag="VF195_07680"
     CDS             <1..1045
                     /locus_tag="VF195_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX6580735.1"
                     /translation="GDWVCIGRAEEVANPGDYLVRDLVEESIFITRNAQGELHGFYNV
                     CSHRGTKFLDDEPASGNVRKAFVCPYHSWTYDLDGRLIGTPNVREDEHFDRGQYPLHG
                     FPVDTYAGFLFANLAREPRPLLESLNDGVESVTVFDRFGMEDLRIGARIVYEVKANWK
                     IVVENYNECLHCPVVHPELVQVVPLFRFGEVWDGETRDGGNEMMDGAKSFTMTGRSEL
                     PPLPGLLDEDMTRYYGAYQFPNLLLNIHPDCVMYYIGYPKGPGHTTVVSEYLFRPETI
                     ADPAVFKPEPIVELWDLISKQDWDVCERAQIGVGSRAFTTGVYPRQDRYLFAFNESYR
                     VKMGRPKLGAARE"
     gene            1104..1595
                     /locus_tag="VF195_07685"
     CDS             1104..1595
                     /locus_tag="VF195_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580736.1"
                     /translation="MAIKRMNHAVLYVRDAEKTYEFYRDVFGFRVVMEMPGAKFLQAP
                     DSDNDHDLGLFTIGAEAGPSQAGRATVGLYHIAWEVQTLADLREIAGKLSEAGALTGA
                     TDHSTTKALYGRDPDGLEFEVCWIVPKELHTSEIEDGRARMRALDIDAEIERFGLETV
                     GGV"
     gene            complement(1599..2366)
                     /locus_tag="VF195_07690"
     CDS             complement(1599..2366)
                     /locus_tag="VF195_07690"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6580737.1"
                     /translation="MKRWASELGPPLLGIAVLVVVWAIVAAATETNSLPGPAEVWTAL
                     RGGFSDGVFVEATIKTLIRLAFSFAAALVIGTAIGLILSASELARRSVRPLVVALQIT
                     PFVAWVPLALIWFGVTERAVVFVAIAGAFPAVTLATLQAIRQVPPLYVRAGRTLGATG
                     WDLQRSVVFPAALPGYLSGVQQAWGFAWKALMAGELIVQASGASGLGHLLDRSEDVAS
                     ILAVVAVIAVIGVAVEYLIVGAIDRRIRSKRGLLESG"
     gene            complement(2363..>2639)
                     /locus_tag="VF195_07695"
     CDS             complement(2363..>2639)
                     /locus_tag="VF195_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580738.1"
                     /translation="DALARERLQDNVQRERIERAGRTTFLFATHNVREAVLLADRVLV
                     MSAAPGRLLEEFRIDAHRPRTLDDVLVARVVSEIHDLLIGQLEKSSS"
BASE COUNT          464 a          877 c          896 g          402 t
ORIGIN      
        1 cggcgactgg gtctgcatcg gccgcgccga ggaggtggcg aaccccggcg actacctggt
       61 cagggacctc gtcgaggagt cgatcttcat cacgaggaac gcgcagggcg agctccacgg
      121 gttctacaac gtgtgctcgc atcgcgggac caagttcctc gacgacgagc ctgcgtcggg
      181 caacgtgcgg aaggccttcg tctgccccta ccactcctgg acctacgatc tggacgggcg
      241 gctgatcggc acgccgaacg tgcgggagga cgagcacttc gaccgcgggc agtacccgct
      301 ccacggcttc ccggtcgaca cgtacgccgg gttcctgttc gcgaacctcg ctcgggagcc
      361 gcgaccgctc ctcgagtcgt tgaacgacgg tgtcgagagc gtgacggtgt tcgatcgctt
      421 cggcatggag gatctccgga tcggcgcgcg gatcgtgtac gaggtcaaag cgaactggaa
      481 gatcgtcgtg gagaactaca acgagtgcct ccactgtccg gtcgtccatc ccgagcttgt
      541 ccaggtggtt cccttgttcc gtttcggcga ggtgtgggac ggcgagaccc gcgacggcgg
      601 gaacgagatg atggacggag cgaagtcctt cacgatgacc ggccgctccg agctcccgcc
      661 gcttcccggt ctcctcgacg aggacatgac ccgctactac ggggcctacc agttcccgaa
      721 cctgcttctg aacatccacc cggattgcgt catgtactac atcggctatc cgaaagggcc
      781 ggggcacacc acggtcgtct cggagtacct cttccggccg gagacgatcg ccgatcccgc
      841 cgtgttcaag ccggagccga tcgtcgagct ctgggacctg atctcgaagc aagactggga
      901 tgtgtgcgag cgggcgcaga tcggcgtggg gtcgagagcg ttcacgaccg gcgtgtatcc
      961 gcgacaggat cgctacctct tcgcgttcaa cgagagctac cgggtgaaga tgggccgtcc
     1021 gaagctcggc gcagcccggg aatagacgtc ggttgtaccg tgtgacgggc gcgatccgtg
     1081 cccatgtcga agaggtgtga ctgatggcga tcaagcggat gaaccacgcc gtcctgtacg
     1141 tgcgggacgc agagaagacc tacgagttct accgggatgt gttcgggttc agggtcgtca
     1201 tggagatgcc cggggcgaag ttcctccagg ctcctgactc cgacaacgac cacgacctcg
     1261 gtctcttcac gatcggcgcc gaggcgggcc catcccaggc cggtcgcgcg acggtcggcc
     1321 tttatcacat cgcctgggag gtccagacgc tcgcggatct acgagagatc gcggggaagc
     1381 tgtcagaagc gggagcgctc accggcgcca ccgatcacag cacgacgaag gcgctctacg
     1441 gccgcgatcc cgacggcctc gagttcgagg tctgctggat cgtcccgaag gagctgcaca
     1501 cgtccgagat cgaggacggt cgcgctcgga tgcgcgcgct cgatatcgac gccgagatcg
     1561 agcggttcgg tctcgagacc gtgggcggcg tctgagggct acccgctctc gaggagtccg
     1621 cgcttcgatc ggatgcggcg gtcgatcgcg ccgacgatca ggtactcgac cgcgacgccg
     1681 atcacggcga tcaccgccac gaccgccagg atcgaggcca catcctcgga gcggtccagg
     1741 agatgtccga gaccgctcgc gccgctcgcc tgcacgatca gctcgcccgc catcaacgcc
     1801 ttccacgcga agccccacgc ttgctggacg cccgacaggt agccggggag cgcggccggg
     1861 aacaccactg agcgctggag atcccatccc gtggccccga gggtcctgcc ggcccggacg
     1921 tacaacgggg gcacctggcg gatcgcctgc agggtcgcga gcgtgaccgc cgggaacgcg
     1981 ccggcgatcg cgacgaacac caccgcccgc tcggtgacgc cgaaccagat caacgcgaga
     2041 ggcacccagg cgacgaacgg cgtgatctgg agcgcgacga cgagcgggcg cacgctcctg
     2101 cgggccagct ctgaggccga caggatcagg ccgatcgctg tgccgatcac gagcgccgcc
     2161 gcgaacgaga acgcgagccg gatcagggtc ttgatcgtcg cttcgacgaa gacgccgtcg
     2221 ctgaagccac cccgtaacgc ggtccagacc tccgcaggtc cggggagcga gttcgtctcg
     2281 gttgccgccg ccacgatcgc ccagaccaca acgagcacag cgatgccgag gagcggcggc
     2341 cccagctcgc tggcccaccg cttcacgacg agctcttctc gagctggccg atcaacaggt
     2401 cgtggatctc ggacacgacg cgggcgacca gaacgtcgtc gagcgtccgc ggccggtggg
     2461 cgtcgatccg gaactcctcg agcagccgac cgggtgcagc cgacatcacg agcacgcgat
     2521 cggcgaggag caccgcttcg cggacgttgt gcgtggcgaa gaggaacgtc gtgcggccgg
     2581 cgcgctcgat gcgctcgcgc tgcacgttgt cctggagccg ctcccgggcg agcgcgtcg
//
LOCUS       DASUYD010000144        44746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1619981, whole genome shotgun sequence.
ACCESSION   DASUYD010000144 DASUYD010000000
VERSION     DASUYD010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 44746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44746
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1619981"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="VF195_07700"
     CDS             <1..432
                     /locus_tag="VF195_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580739.1"
                     /translation="VPTAAAIPCLDALPSGWIYGGGDIHSGEASFWLNSDLAGMRAVT
                     VTLRSACDVSSAHEVPSDEAGARRFEAPSSLAPQLLNVRSYVIPGGCATYRFAFTTGA
                     PTALVFDVDEAVSFVPRSVLVAHLRETEGLALCGRGAPCPP"
     gene            492..1136
                     /locus_tag="VF195_07705"
     CDS             492..1136
                     /locus_tag="VF195_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6580740.1"
                     /translation="MPRRDTVVTAALAVATATLFALMATDAGRDLIQPIDDAFLRWVR
                     SVRTAWLTLIAKIFNLIGLTYVMTPVRIGVAAFLAIRRRWWHFIAFVSAIVVSEISIG
                     TLKALYDRPRALGSLVETSGASFPSGHAVAASITAVAAVIALFPEGSRRYRWGAAAVA
                     FSSVMGLSRAYLGAHWLSDAVTGVLLGTSIALGTALIVHRVRERRASGDVASLV"
     gene            1352..3259
                     /locus_tag="VF195_07710"
     CDS             1352..3259
                     /locus_tag="VF195_07710"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX6580741.1"
                     /translation="MLELLVSLLITILAVTLVRRLLGIERGRWVVTVLAVLVGEVFAV
                     LVLRTGVGNIRDLPPRAAFGAYALVTVFAMLGIVVVELIARPGARRLRGIPRPIRGTR
                     HLAGRAVRYVKVSVIAARHGLINPAGSDVDVRGSRLGSALSASFEDAGGLFVKLGQAM
                     AAQPHLVTRAVAAELARLQDRAAPADPAAARAVIEQELGLPEEIFTDLPSELLGSASI
                     AQTYAARLRDGREVVVKVQRPGVRESIEQDLDILARLSNRLERRTTWARSLGLKELVA
                     GFAERTREELDFHIEATRTAAFRRSLQESDRVHVPEVFEELSTGRVLVQERIVGQSVG
                     APGALEGIDPDRRRGLADGLLSMTLRQMLAGEPFHADPHPGNVFVDPDGRLALIDFGA
                     MGRLDRFERSGLIDMMRGLQTEDPSLLREAALRIGTHTTRIDKEALDRELARLLGGAI
                     KPDGTVNPDLFNDVLFVFRDFGILLPRSTTTLFRTLVTLLGTLQVIAPGHNILDAARR
                     LGGGVVAEQMAPGNLQELVLRQAMDAAPVLARLPRELDELARTLLHGELRTRLSLLSE
                     PEDVRVARGMANRLVMGLVGSALALSSSVLLVAPSPVGPGLLNILGGIGMFFSVLLLL
                     RLVVQILRERD"
     gene            complement(3283..4509)
                     /locus_tag="VF195_07715"
     CDS             complement(3283..4509)
                     /locus_tag="VF195_07715"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580742.1"
                     /translation="MSGLPPSGPSRGLSAEELANQAEVPVDFVHRLIELGGLRANRDG
                     SYPLSDVGRIRILHAWEEAGLSPEGIMELVSGGDLSISWLDAPVMTTTARLDATFEQL
                     CSEAEVPHATIRSLYESLGFAPPEPTDRIKTGDSELVDLLQAFFAAGAREGPTLRLLR
                     VYADSLRRITKAEAELYESEIEEPLRRSGRTERELLDLGARFGGEVNAQLERTLLDIY
                     HRHREHIWIEHSINHAEIALEHAGLFEKVPKPPAICFVDLTGYTRLTEERGDASAAEL
                     ASQLASLVEDISRRRGGRPIRWLGDGGMFHFKDPQAAVLAGLDMVESAPGADLPPMHI
                     GIHTGPVVFQDGDVYGRTVNLASRIASHAAGGEVLASGETVRRSTGGVRFEAIGSVAL
                     KGLTHPVDLYQAFRPR"
     gene            complement(4560..5147)
                     /locus_tag="VF195_07720"
     CDS             complement(4560..5147)
                     /locus_tag="VF195_07720"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX6580743.1"
                     /translation="MLRRPATEILDRFRAGGSSLILLWWMFMLSGLLLIPAVVLLGQA
                     LGFTGVVPLAVSIGVLAGLVQMLGLLRWVYLVPSLARMNGDLALGIGQREANIAVFSA
                     MHRYLGVGVGEHLGYLFTGIWSGLTGAAAVGSQMIPAWMGWVGIVIGAGLIVGSGEFL
                     GRDDEDGWAPAGAAIPILYAAWSVWLLAIGIALII"
     gene            complement(5166..5648)
                     /locus_tag="VF195_07725"
     CDS             complement(5166..5648)
                     /locus_tag="VF195_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580744.1"
                     /translation="MSATTVSTNEATRSEDRPGDGRGIARRLWLWPFAILVSLPIGGY
                     VADLAVDAIDSVGAALGGGLIVGAIVGVAEWLALRRWVSWVWVPATIAGMAAGLVAGA
                     ALVDYGIGRPDLVLMGAVNGLAVGVVQALVLARHRVPGAFWWAVANPPAWALGWFVSS
                     "
     gene            complement(5680..6609)
                     /locus_tag="VF195_07730"
     CDS             complement(5680..6609)
                     /locus_tag="VF195_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580745.1"
                     /translation="MASRGRSHTLIRSLTLAGVLLLMFAGPASAQEDGSGADDTDQVV
                     LTGRLLVEGDRTVDTAVIFNGPATIEGIVREDLFVLNGDTEVSGRVNGDVVVFNGDVT
                     VRSGAEIGGDLVTQGTPTVEDGATVRGSRSNITTKFDYDMAGFAGRFVWWLGYTVSVL
                     ILGLLLLAFAPRLDEAVVGTIQARLGSSIGWGIGLFFLLPVAAVLLLVTVVGIPLGLF
                     VLLGLGLIYTLGYTVATLGVGRLVMRSSSRYGAFLVGLVILRALGLIPILGGVLWLLA
                     SAWGLGLLAVAIRAGRIERTVAVAPPPPMPVAG"
     gene            7024..7512
                     /locus_tag="VF195_07735"
     CDS             7024..7512
                     /locus_tag="VF195_07735"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial transcriptional activator
                     domain-containing protein"
                     /protein_id="HEX6580746.1"
                     /translation="MATTARDLGLSDDVTVDLWDARALAHQLLTPTPASPSVDPGPEA
                     IPTLSAELLPDWYDEWALVEAEDWRQLRLHALEALADRLSAQGRYGDAAAAALAAVRA
                     EPLRESPRAALIRVHIAEGNPTEALREFARYGELMMLELGVEPTERLRALVADLWAVT
                     QP"
     gene            complement(7753..8823)
                     /locus_tag="VF195_07740"
     CDS             complement(7753..8823)
                     /locus_tag="VF195_07740"
                     /inference="COORDINATES: protein motif:HMM:NF015552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance/C4-dicarboxylate
                     transporter family protein"
                     /protein_id="HEX6580747.1"
                     /translation="MSARAATERTSPIATLFPGSFALVMATGIVAIAAYQQGFETLAR
                     GFLAVNVIAYVVLWMLTIVRLVRFPGRVRDDIATHQRGAGFLTIVAGTNVLGSGVTLL
                     TSWDGLALGLWIAGLVLWFVITYWFLTAVTVRAEKPPLESGIGGAWLLLVVSTESIAA
                     LGALLAPSLGGSRGLLLVSILAHLVGLMLYVVVIGLIFYRWTFFPLSGEQVTPPYWIN
                     MGALAITTLAGSNIVVASGSWETSVGLVPFLTGTTLLAWGFATWWIPLLLAIGVWRHG
                     VQRVPLRYDPQYWSLVFPLGMYAASTFRLEAALHVGLQSAIATTAFWIALAAWFVTAA
                     GLIGSALLPRRARDDMSVESSR"
     gene            complement(8820..9089)
                     /locus_tag="VF195_07745"
     CDS             complement(8820..9089)
                     /locus_tag="VF195_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580748.1"
                     /translation="MIGDRRRHFEVAPGPGPGIEPRANRQRQAVIVARIAIVATIVVG
                     QLWALTIALDAYLLEEMGTVRWLLAFQGVSFVLALAVWWSSPTDR"
     gene            complement(9086..9583)
                     /locus_tag="VF195_07750"
     CDS             complement(9086..9583)
                     /locus_tag="VF195_07750"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6580749.1"
                     /translation="MTRSEPTGTDVAEERRQLERITSEGLVSRRDYLRILVTISGGLL
                     AGAVAVAAGLFPRRTGTATPVLVAASLGEGEWVRFAYPTEEDRAIAMRLDGGQLVAYS
                     SICTHLSCAVIPKPGEQVLDCPCHEGRFDATDGSVVHGPPPRPLPVIELEERSDGIYA
                     VGATP"
     gene            complement(9580..10170)
                     /locus_tag="VF195_07755"
     CDS             complement(9580..10170)
                     /locus_tag="VF195_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019766142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6580750.1"
                     /translation="MMNRTLFIDPGRCIGCQACVAACRECDSHRGKSMIHLDYLEEGA
                     TVASMPTVCMHCEDPVAPCAQVCPTDAILVTADGVVQEAAKERCIGCANCVHACPFGV
                     PKLDADEVLMYKCNLCYDRTSEGLQPMCASVCPTGSIFYGTLEELLNARPNAAASDIV
                     VFGEEEVRTGAAVVVPAAPVGVPAGAAGAVPGGFTP"
     gene            complement(10167..12380)
                     /locus_tag="VF195_07760"
     CDS             complement(10167..12380)
                     /locus_tag="VF195_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014139914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HEX6580751.1"
                     /translation="MAPTELETPPGSKLVPTHCCYCGVQCGMYLKVDARGRVFGMEPR
                     DHEINRMKLCPKGVTAYQQVNHPDRLLYPLMRDARGQPLRRASWDEALDRVASEIHRI
                     QETYGRDAFAVYSGSSLATEVTYLMGKFARVALRTKNIDYNGRLCMVSAAAANKKAFG
                     IDRAANPWSDMLDTQVILVAGSNVAECFPVMSNYVWAARDRGAKLVVIDPRETPLART
                     ADLYVPLRPGTDSAFFNGVLHVVAREGWLDEPFIAERTVGWADVREAVAAYDPDRAAE
                     ICGIEPRLVEELARMWGTAERAMAFHARGIEHQIQGVENALSIINLVLATGQIGAPGK
                     GYGTITGQGNGQGGREHGQKTDQLPGQRDIEDPAARAFMSRYWGIDEADLPHTGASAV
                     ELVHLMGTEVRGLLGICNNPLVSMPNATRIAANYDALEFHVQLDFFLSETAERADMVL
                     PSAVWAEDGGVTTNAEGKVVLRHTAAEPPGEAKRDWWIVSQIAERLGVGEKFRYAGID
                     DIFEELRWATEGGNADYRGITYERLEREGAISWPCPSEDHPGTPRLFEERFSWPDGRA
                     RFNRVEWQQPHEPVDGEYPLRLTTGRTVAHFLSGNQTRRIAPLVEQAPRPWVEVHPSL
                     GFANGDPVRVVTRRGEATYPALVTEAIREDTVFVPYHWAGAASANLMTVDALHPISKI
                     PEYKVCACRVEQGDAITPAPPPPLAPGQAVDVVAARAAADDRPPTSMQGRGTGES"
     gene            complement(12430..13470)
                     /locus_tag="VF195_07765"
     CDS             complement(12430..13470)
                     /locus_tag="VF195_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018524898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6580752.1"
                     /translation="MDDRRPPSLMQAERRASVAGIVTAGLVAMAVYFGSGRLRTFDAA
                     LIGYATATLVLVFGVVYRYTIWIQQPPTRRYFLKGWRAFLSWRNFRRFPTLVPKQVVS
                     YLGFQTFLRERSRGRWLAHVAIFWGVVLAALMTFALSWGWIHFRADDVGSYQMYVWGL
                     RVLTFEPFTWFGWIVFHLLDVAAVLVIAGSGYFLWRRIRDREVRSEQRLGYDFVPLIA
                     LIAISVTGLLLTFSSVLLGGAGYEFLAILHMGVVVLSLVFIPFGKFFHVIQRPAVLGV
                     QVFKHVSATEDGVTRCRRCGEPLETAAFVRNLQETMDELDLRYRAWVETCPRCKRVQR
                     GQAYLRDVKRGF"
     gene            complement(13679..14431)
                     /locus_tag="VF195_07770"
     CDS             complement(13679..14431)
                     /locus_tag="VF195_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580753.1"
                     /translation="MPVADASSGWLEAAGAFGVLSVVAFAVTWIVTDLARVPRTPYVA
                     VLTVTTLVLGVGYVAWSGTEVADLLTDEWGWGIVGGIAAAAVVVPLVRRLPSRPHPEG
                     PRLVGRLLWEGVVYGMAEGLLLATLPVIAVWQGAEALGWTDLEVGAIASGALAIVGAL
                     IVIAVHHLGYREFRRSRKMLAGALAACGIQALAFLLTGNVLAPVLAHIVLHGQLTLRG
                     DEMPPSKASQEPGRAGRITTVEGLPRPKELVA"
     gene            complement(14711..15088)
                     /locus_tag="VF195_07775"
     CDS             complement(14711..15088)
                     /locus_tag="VF195_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HEX6580754.1"
                     /translation="METTKKVDVDKTIEDAKDAITVRRVFGEPYVKNGVTLIPAARVQ
                     GGAGGGSGEGSNGEGRGGGAGFGLSAKPVGAFVIKGDEVSWRPAVDFNRVILGAELVT
                     LVALLVARSIVRSRPSRTHDRIA"
     gene            complement(15301..15888)
                     /locus_tag="VF195_07780"
     CDS             complement(15301..15888)
                     /locus_tag="VF195_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580755.1"
                     /translation="MDRSSWDKIVSGAGAVIAVVLIALGAMAIYGGNFGQQNVRDRLE
                     PQNIVFPPAEAMSPEEKADLGEFAGEKVDTGAEAEAFSRYIGGHLVGINDGATYSETS
                     SAAREEGLDPDVAAELQGKADTLFKGETLRAILLNAYGWWTVATIALWAGFAMVAAGL
                     LLGIFSILGFRHAARAVATTTEHRVNDPEYAGSTR"
     gene            complement(16296..16670)
                     /locus_tag="VF195_07785"
     CDS             complement(16296..16670)
                     /locus_tag="VF195_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580756.1"
                     /translation="MMWRFLFIAFLVAHALVHLAIWLLPKPPDQKAPFDPNHSWLLGD
                     QRAVAVLLAVTAAVLLMAGGLGLWAHAGWWRGVAAIGLAASFGLMVVYFNPWYLFIEG
                     INAALIVGIVWLTWPSETMVGA"
     gene            complement(16678..16962)
                     /locus_tag="VF195_07790"
     CDS             complement(16678..16962)
                     /locus_tag="VF195_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580757.1"
                     /translation="MSEDLVVQTNGPYLRVVLPDFEPDWAAVWSAGDFELDEGVERAE
                     VVAPCYPDDDCLAGVRALAEQLERRGVQTVVEWEGTPPLAKAEGEQFSST"
     gene            17235..17870
                     /locus_tag="VF195_07795"
     CDS             17235..17870
                     /locus_tag="VF195_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580758.1"
                     /translation="MVYASLFGRLFKIDLVAPEVPRSSVAHRRVVDHALTNEEILFDT
                     GTQPFSKQLADAVETFWRVGVAAQSRTSRPDRGCWLLSPPRGAMSQEIPSVEVRSQED
                     ERDVDRGQGMGEPARQEDVGHGTVFGPERFGNIGQRTAEAVRGEDQTLAADHGGRDRG
                     APVGRFANPAHSRYTMHDSMENQASVWMALTATSARPALIASPLTALRGRA"
     gene            complement(17830..18285)
                     /locus_tag="VF195_07800"
     CDS             complement(17830..18285)
                     /locus_tag="VF195_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6580759.1"
                     /translation="MMNDRVEGEIVIHRPVEEVFDFVADERNEPLYNPRMLHAEQTSP
                     GPVGLGTRFSAEMRSAGRTVKMTIKNTGYERPRRLAVRTHLANMDIEGDLRFEPLADG
                     TRMRWLWHLKPRGALRLMTPLITRIGRRQEQTIWASLKRVLEARSRGMR"
     gene            complement(18472..18981)
                     /locus_tag="VF195_07805"
     CDS             complement(18472..18981)
                     /locus_tag="VF195_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX6580760.1"
                     /translation="MTVLVAYASKHGSTEGIAHAIADRLRELGEPVEVGSVDEVGDLA
                     EFRAVVLGSAVYLGSWMKEAVEFVHRFAEALAERPVWLFSSGPLGADVEDEEEQPRQL
                     GEMGGIIGPVDHRMFFGALDRSKLGFGERMMVKAVKAPEGDFRDWAAIRDWADGIAKE
                     LGASPGTGE"
     gene            complement(19031..19930)
                     /locus_tag="VF195_07810"
     CDS             complement(19031..19930)
                     /locus_tag="VF195_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580761.1"
                     /translation="MAAELVRESRRHNVQDAALDPVRRPGAKPGLRAAYWVSGAIGVL
                     MVVASATGLLVDGLYRDGPWARGALRGGDLTTIAVAAPILVASVLLARRGSRAAQAVW
                     LGALAYSLYNYAYYVFGAAFNDIFVIHIALFSLSIAALVLAVANIDVGAIAARFRDVT
                     GVGVIGAFLAVVGIALGGLWLFLAIRFAMTGELMADLPADGIHLVFAIDTSLLVPALV
                     ISGILLWRRTPQGLVFGTAMAVMGAMYQVNLLMAGVFQANADVPGVKAFPLEGIVLAS
                     GFALATLTLLLDRKADERVLDQS"
     gene            20087..21508
                     /locus_tag="VF195_07815"
     CDS             20087..21508
                     /locus_tag="VF195_07815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX6580762.1"
                     /translation="MREVELETRSLDQLEGLVRPSHLEKFRSLLGTAADRLEGRTLWH
                     VNSTSDGGGVAELLHQLLGYLIEGGIRCRWIVIEGDQDFFWMTKRLHNRLHGSGGDGG
                     PLGEEEREIYMRVLREQLSPLLDLMRRGDVAVLHDPQTAGLIGPLVDAGLGVEWVCHV
                     GVDMPTDIVRSAWAFLRPDVEQAHASVFSRRAYIWEWLDRRKVSVIPPCIDPRSPKNA
                     DLEPTDVDAILGAAGILTMPEPTEALVEIDGVGSVQVHRDSERVEEAPAPADAPLVSQ
                     VSRWDRLKDPVGVLHGFARYVRPNLGAHLLLAGPAMASVADDPEGAEVFAEALAAWQR
                     LPAEPRARIHLVNIPAEDRVENAVVVNALQRKVDVVVQKSLAEGFGLTVTEAMWKERA
                     IVASRVGGIQDQITDGRSGLLIAPTDLPALGKAISSLIADLGFAQAVGHAARQRVRSR
                     FLPPHFLGGHLDVIDRITRGALE"
     gene            complement(21523..21975)
                     /locus_tag="VF195_07820"
     CDS             complement(21523..21975)
                     /locus_tag="VF195_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6580763.1"
                     /translation="MAWSGDLDRVWPTLILGTTGAAMGMETTIFFTFWGLFPLVKDDV
                     RITGDNWMQKLLSTMDRPGLEHVKLGKMNFAGAGPAMMRKLARDHHVASPEELIDLAK
                     DLGVKLVPCQMTMDLLGLHRDDLIDGLEEPAGATTALLEAQDAITLFI"
     gene            complement(22059..22292)
                     /locus_tag="VF195_07825"
     CDS             complement(22059..22292)
                     /locus_tag="VF195_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009793110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6580764.1"
                     /translation="MSATIVKTLDMKGFACPLPIAKTAQAIRELAVGDLLEVLATDPG
                     SVPDFAAWSGSTGHELLERSVDDGVFRFLIRKR"
     gene            complement(22296..23456)
                     /locus_tag="VF195_07830"
     CDS             complement(22296..23456)
                     /locus_tag="VF195_07830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEX6580765.1"
                     /translation="MPARIVVLGGGVGGTLAANLLSKELGRDARVTVVDPTGMHVYQP
                     GFLYLALGEANGRWLARDERTLLRRDVDLAIERAVRIHPDAGSVQLERGGSLDWDYLV
                     IATGARLVPEQIPGLAEGAFGFYSLEDAERLREELRRFRGGRVKVGVAGIPYKCPPAP
                     VEFVFMLDRFLRRRGLRERSEITLLSPLNRAFTIESASKLIQPIMSARGIELETFFNV
                     ESVDASAGVVSSLEGDKAEYDLLVLVPPHRGQVAIDASHLGDPGGWVPTDRHTLQVKD
                     QERIFAIGDGTDLPISKSGSTAHFEAPVVASRIASLVRGTAPRSNYGGRVMCFLETGD
                     GRATTLRFDYEQPPVPPQPHRMWHAAKWLFNRLYWETVPQGRIPEWAMRGKE"
     gene            complement(23461..23685)
                     /locus_tag="VF195_07835"
     CDS             complement(23461..23685)
                     /locus_tag="VF195_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6580766.1"
                     /translation="MTRSIDARGMPCPGPLMSLIGAIREGSIGDEIEVLSTDAGSLTD
                     IPAWVKKASHELVSMVETDGATSFVVRKMR"
     gene            complement(23722..24132)
                     /locus_tag="VF195_07840"
     CDS             complement(23722..24132)
                     /locus_tag="VF195_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX6580767.1"
                     /translation="MDTDEDRLSLVLCSGTDDRLTGAAVLVVGAAAMERKVEILLQFW
                     ALDAFRADRIMKDHGLSPEAGTDGQTLWEWHEGTHWSEMLREAKEIGDVSIHACSLSM
                     DLLRITPDDLDPLVDDVRGVASFLAAAGGAITFV"
     gene            complement(24312..26981)
                     /locus_tag="VF195_07845"
     CDS             complement(24312..26981)
                     /locus_tag="VF195_07845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6580768.1"
                     /translation="MGDAETPTDLEKDVVLRDGRTVHIRPARSEDRGVVEDYLIGLSD
                     ESRRLRFWSASIDVTDLAAKAVRPEYPDHLTLLAVTGSDAGHVVGGAQFVRENATRAE
                     LSVSVSDELQGSGLGSLLVGNLVDAAYGAGISVLEAKVLPENLRMIDVFRQSGFPISI
                     RAKPGAVSVEISTAPTEEALTRFEEREAVAAASAVRTILEPETVAVIGASRDETSIGG
                     RLLRNLLSHPFHGTVYPVNPSAPAVQGVKSYATIEDVPGPVDVAFIAVKASLVAEVAE
                     ACGRRGVRGLVVISAGFAETGGDGPARQRELVEICRRWGMRLVGPNCMGVVNTDPRVA
                     LNGTFATIAPEAGSVGFLSQSGAVGLAVMSETRRRDIGLSSFVSVGNKADISGNDMLS
                     YWEDDARTNVILLYLESFGNPRRFSHLARRIGKRTPIVAVKSGRTQAGQRAAGSHTGS
                     LVATSDLTVDALFRENGVIRTDTLAEMFDVAMLLATQPPPAGNRVGIVTNAGGLGILC
                     ADTCEANGLVLPPLSQETVDELRTFLPSEASTGNPVDMIASATGEDYERTLIAVAGDP
                     SVDIAIVIYIPPLERHADDVARGIGEAIASVGGSTPVLTSFMSGGEVPVDPVSGRRWF
                     PSFAFPEQAAIAAARAVEYGRWRDRSPGTVPRLIDVLSDEADAILAAALSRGSGWLEP
                     EEVDGLLACYGIPVAMSRLAITPEEAGRYAVELGVPVALKAVGPLHKTDVGGVRLGLS
                     GKDAVTAAAEEMQERLGSLGGPLKGFSLQEMVEGVEMLVGVTHDDVFGPIVACGAGGT
                     TAELLKDVAVRVAPVTDTGAREMVRSLKTFPLLDGYRGAPRANVLALEEIILRVSALA
                     ADHEPVREMDCNPVMVGETRALVVDSRVRVAAP"
     gene            complement(27074..27487)
                     /locus_tag="VF195_07850"
     CDS             complement(27074..27487)
                     /locus_tag="VF195_07850"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6580769.1"
                     /translation="MLERILVATDSSEPSKRAVEFAAGLASDHGSEIIVLHAVPRIVS
                     RYGTSYLEEPDAATEVVDEVVRDLKDRGLNARGEVVRVLEGHMPRGIVESAASADVDA
                     IVMGTRGRSDFGGLFLGSVTHRVLHLSEKPVIVVP"
     gene            27710..28852
                     /locus_tag="VF195_07855"
     CDS             27710..28852
                     /locus_tag="VF195_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6580770.1"
                     /translation="MLDGSTKDGDRRLIEAGMILASELSLDAVLLRIVELAVDLTGAR
                     YGALGVLTPEGRSIEEFITVGITPEERAALGDPPTGHGLLGALITEGRPLRIPDISAD
                     PRSVGFPPNHPPMKSLLGAPITGRGKVFGNIYLTDKQGAETFDEEDERVLIVLATQAA
                     IAVENARLYDETEGKSRELQRLQVVEERERIGKELHDGVIQSLFAVGMSLQGLASSSS
                     DADTARRLEAAVEDVDHAIRDLRNYIFGLRPGILADRQLDQALKEMASEFGARSGVVT
                     VVDVDSFAASALASHAADVVQLAREALSNVSRHGAATTCRIALLRGESGLFLEIDDDG
                     RGFDVGVTSWGMGLRNLRERVESLGGVLQVDSTPGEGTTVRATFPS"
     gene            28921..29424
                     /locus_tag="VF195_07860"
     CDS             28921..29424
                     /locus_tag="VF195_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580771.1"
                     /translation="MFARYFVELPMSAEDIERALSRDPRVWLPGLAARAHHRGDLLLA
                     EVGFGEAVRVKRTVAVELGQPVRSGSKTVFPLRWAASGPGGLFPSLDADLEVATLRPG
                     HTQLAMSARYVPPFGTVGRVIDRAALSRVAEATLKDFLDRVADAIMGSGDVPVPVELG
                     GTAPASA"
     gene            29448..30122
                     /locus_tag="VF195_07865"
     CDS             29448..30122
                     /locus_tag="VF195_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008740031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580772.1"
                     /translation="MAKPKSPPLRVMLVDDHEVVRDGIRAMLASEDDIHVTTEAGTVR
                     EAIDEADRTRPDVVVMDVRLADGSGIEATREIRANHPATRVLMLTSFADDEALFASIM
                     AGASGYVLKQVRSGELVRAIRAVGAGESLLDPAMTTSVLDRLRKGKHLMRDERLSRLS
                     PQEERILTSIAEGKTNREIGDELTLAEKTVKNYVSSILSKLEVARRAEAAAYLARHTT
                     MPGSVE"
     gene            complement(30111..30554)
                     /locus_tag="VF195_07870"
     CDS             complement(30111..30554)
                     /locus_tag="VF195_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580773.1"
                     /translation="MPHEGTVLLVESDDAERERLGMALEQAGYQVIACPGPTAPDYTC
                     IGGREGYCPLLERADVVVLDPWLAGDELGVGTSSDELLALYTGSGRTVVAIDAAPLDP
                     VARGRMIRLDRRPGAETVVAAVRVAAEADGFVLRRPVGDGDGLLD"
     gene            30679..31332
                     /locus_tag="VF195_07875"
     CDS             30679..31332
                     /locus_tag="VF195_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580774.1"
                     /translation="MKLIKEVGQMETIERRALDIPALERLEPIHTKGLQRVFGALRLV
                     AGWTFLWAFLDKAFALGFSTGRILNDAGQTVKIDFFGPEAWINGASPTAGAVGFGLKG
                     PFADNIQSITNFQMTQAGPQVAGWLDWVYMGSMLLIGLGLMLGVGTRLAALGGIAWMT
                     IFYAGTAIWPEHNPVVDDHVVIAVVLVGLMLANAGRYYGLGKVWQRVGFVKDRKYLY"
     gene            31594..32163
                     /locus_tag="VF195_07880"
     CDS             31594..32163
                     /locus_tag="VF195_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEX6580775.1"
                     /translation="MTRALVVFESMFGNTQQIAEAVKEGLSSYVLADILEVGTAPDVL
                     PNGVDLIIVGGPTHAFGMSRPRTREDASRQAGGHVVSERNGLREWLTTVERTGRSLAA
                     AAFDTRIDRPRVPGSAARGAEKRLRKLGFRIACPSESFYVTGTTGPLVEGESERARRW
                     GEGLGKGSLGVLDGAEVAEVRSEDVTSGR"
     gene            32178..32906
                     /locus_tag="VF195_07885"
     CDS             32178..32906
                     /locus_tag="VF195_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580776.1"
                     /translation="MRRVTLVLISSLMASLAVLAALIVWLGWVLGAAIGLTIAIVVVT
                     LYITVVGPWQRRWGTTDAEARGPMPGDDLLRPGAPSTTRAITIDAAPRDVFPWLVQIG
                     YERGGWYSYDWIDNDGRRSIDRIDPTLQDLSVGDRIQMFPGFGPVVREIVPEHHILSG
                     GDADTWCLLLEPTAEGRTRLVSRWRQDWPKTFGTFAWALISDPGAFVMERKMLRTIRS
                     LAEARRVGGRMGRSTTSPRGSGPP"
     gene            33088..33273
                     /locus_tag="VF195_07890"
     CDS             33088..33273
                     /locus_tag="VF195_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580777.1"
                     /translation="MASGSTFGLGPTRWLSGIAFSLGVFVIVAGAERFTGNNLIVMRV
                     ATGHVKIRRLPQNWDEP"
     gene            33270..34172
                     /locus_tag="VF195_07895"
     CDS             33270..34172
                     /locus_tag="VF195_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580778.1"
                     /translation="MTGEVSIALHGLTKRYGTRRGIEDVSLTVAPGEVFGFLGPNGAG
                     KTTTIRTMLDLLRPSAGRATLLGLDSHDDAVRIHRSVGYLPGDFVAYRRMTGRRYLEF
                     FAEVRDVPFGTVEALSRRFACDLEPQIASLSHGNAQKLGVIQALMHRPRLLILDEPTQ
                     GLDPLIQRVFHELMREIAAEGRTVFLSSHVLPEVERMCDRVGIIGDGRLLGVEDVGDL
                     RAKAIRVLEFHFATRVPATVFADLPGVSDLEVHGETVRCSVRGSVDGVLKAAARYEVV
                     DLRSHEPGLEELFLEYYPTAEEAS"
     gene            34169..34963
                     /locus_tag="VF195_07900"
     CDS             34169..34963
                     /locus_tag="VF195_07900"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6580779.1"
                     /translation="MIDLVRKELRDQRRALIGWGIGLSAMVLMYSSFWPSVRDNADQF
                     ESYLENIPDALRSVIGELSLGTPVGYLQSELFSFLGPALILVFAIGAGARSLAGEEEQ
                     GTLDLLVVTPITRRMIVLQKFAAMVLTALGLGVMLWISVVVVGRLFGLDVAIPDVAAA
                     ILHLLLLGVAFGAIAMFLGASRGRRALAIGVTSALAVGTFILNAFAPTVDGFQWAARL
                     SPFFYYGDTLPLRNGLHIGHAGVLVALAAVAVIATVASFDRRDLLT"
     gene            complement(34998..35615)
                     /locus_tag="VF195_07905"
     CDS             complement(34998..35615)
                     /locus_tag="VF195_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX6580780.1"
                     /translation="MIRRPARPRGFGGGSWRQAGFASLDFETTGLDLQGDDIISFGVI
                     PIERGRILPGRSTYREVRPDRPLKPASIAVHGLRPVDLAAALPLGAVVDDLHDALAGR
                     YVLAWAAEIEAAFLARAFGRRPSWWRRHIVDVLRLALLVDRSGSRNDDFSLSAVAERL
                     GVPVEHPHHALDDALTTAEVFLVLLPDLERRGIMTPRRLLRAGRG"
     gene            complement(35612..37450)
                     /locus_tag="VF195_07910"
     CDS             complement(35612..37450)
                     /locus_tag="VF195_07910"
                     /inference="COORDINATES: protein motif:HMM:NF021824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEX6580781.1"
                     /translation="MQVRSFLVRFPPFDVLEPEALERVCAATEIEFFPRGATVLQQGG
                     PPSRFLYVVRTGGVELLDDEVVIDLLEEGEMFGHPSLVAGLPPTLSVRAFEDTICYLI
                     ERQVAEEVIGTRSGIEFLARGLARRSRQAALASETERIARSPVPLSSILHREPIGCVP
                     TLSVRDAAATMSREAISSILVRTDELLGIVTDRDLRTKVVAEGIDLSVPVARVMTTPV
                     ITVSAEAMADDALRLMLEHGIHHLPVTGSDGGIVGVVSDTDLIGLERTVPFTFLRALD
                     RAETTKEVFARMSELPAVVIPLVEIEADPVDIGHLIASAIDATTRRLIHLAIGQLGDP
                     PGPWAWLALGSEARHEQSLVTDQDHALIFVAPDRSPEEADAYFERLARRVTDGLAGAG
                     IPRCRGGVMAESAAWRADRETWIRRFREQLGLSDVGGTAFSNIALDYRRVAGALDVEA
                     TIDQLVRQAAAEGWLVRKLASSALDLRPPTGFFREFLVGWDGAHAGTLDVKHGGITPI
                     TNLARTFAVASGVASNRTRDRLRVAASLGALDPDLAVGLEEAFRLLWRIRLRHQVSQL
                     RAGDPADDHVDPRMLGPLTRRSLREAFKLIIAGQRVLSTTYGLRPR"
     gene            37690..38196
                     /locus_tag="VF195_07915"
     CDS             37690..38196
                     /locus_tag="VF195_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580782.1"
                     /translation="MGGPVVLLGFGLAIWWGTATYLEMVDDLARLHRIRIPSARVVVL
                     DEGTQILSVESGRLATFTDLRFAVVGPDRAAVAVDPYERDLRYDVPDEPGREGRAVAT
                     FEADSPGRYTVRVAGQAAAGAILAIGEDASRAALPEILGAMALMAASLVGGAILLALR
                     IARWGDGR"
     gene            38193..39515
                     /locus_tag="VF195_07920"
     CDS             38193..39515
                     /locus_tag="VF195_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6580783.1"
                     /translation="MSAPADTRAPARPSAIHRHGRLRPSAPGTSRLAAAACGAVVLYG
                     LAALVVLPEGDPSLLARLISAAALVTAASVAVGVIRWGAARAGGAVILLVGFTMMAVA
                     GGVVAKRLVDDVSLVDLLGAAASIAGVILVVAGWKLSLAGIARRWMRPTIAILATLLV
                     AQFLLLPAGVALDVTNRPRPTGSGRTPADVGLRFQDVRIPSADGTLLSAWWIAPENGA
                     AIILLPGSGSTRHTVLDHAALVARHGYGALLIDWRGHGESEGRSMEFGWGAEPDVSAA
                     VSFALGRPGVTRGVGVLGLSLGGEVGLAATALDPRIGAAVAEGISARSWADARLEPDP
                     HPVGYVNQWLTFVFVDILAPEPRPLPLIDLITRIDAPVLMISGAPPSERKLDPVYADA
                     APDVITLWPLPDTPHTRALGTHPAEYERRVIGVFDGALLDNGAVAPNG"
     gene            39693..40205
                     /locus_tag="VF195_07925"
     CDS             39693..40205
                     /locus_tag="VF195_07925"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX6580784.1"
                     /translation="METSIDVPAVPKPIGVGPEMEALWRFFRNCTWEGTIHEGGMGPG
                     TPAMRGAGRGVVEPLQDGRWIVGDFEQDQFLEDGTFVLTWQLHWVSGWDPEHGEYRAS
                     MVDNYGHAAVYRGYIDGDRLVFESMGDTGPRLRFTWDASDPDVTTWRNEMAIDEGPWF
                     LIEEYPMVPA"
     gene            complement(40711..41046)
                     /locus_tag="VF195_07930"
     CDS             complement(40711..41046)
                     /locus_tag="VF195_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013837410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580785.1"
                     /translation="MRSSGTLGEYRYVKRTWLFVAAAVVLSLAMASCAPGPNVQEGAA
                     QSGEIAGFWLGLWHGIIAPITFVISLFSDDVNVYEIHNNGNWYNFGFVLGAGVLLGGG
                     GAGSRGRRG"
     gene            41395..41907
                     /locus_tag="VF195_07935"
     CDS             41395..41907
                     /locus_tag="VF195_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580786.1"
                     /translation="MSEIATLNDRLNDLRSGLTEARGAVAALEGSLSAAEAELGAMLG
                     PTLPDGKHFGALIALGAAQQPPRLVIDIQQWFTDEEAIAAAIEDGVIEPGEIIPNGYY
                     IRNDNPRWRTIEIDPAAKVSLTTYPYGQIDDPQIVSLERFADLAIDWSEYWITMAEGK
                     VVAIGQQYIP"
     gene            42012..42338
                     /locus_tag="VF195_07940"
     CDS             42012..42338
                     /locus_tag="VF195_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018770439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580787.1"
                     /translation="MQWDLGLRGLGLLVLISLGFGVVAQLIGGKATTHWLWLIAGAAY
                     FLAGLFISEVWFGWATEKELQPNIDGLSFDEVLIGLVPGILAVLVTRKVSRSRSHPSH
                     RSRIVY"
     gene            42538..42726
                     /locus_tag="VF195_07945"
     CDS             42538..42726
                     /locus_tag="VF195_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580788.1"
                     /translation="MTGIGIPPIDPGITGGGGSIWPEIVAAIVLLVVFAAAIWTYIRN
                     RPAHTDRTVSQTELPKAA"
     gene            42830..43108
                     /locus_tag="VF195_07950"
     CDS             42830..43108
                     /locus_tag="VF195_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020251809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HEX6580789.1"
                     /translation="MSEQRQQREWTEELKVQGEALLGTVKELAHEGNVRRIIIKDAHG
                     QTLIEIPLTVGVVGALVAPVAAAVGAVAAMATDCSIVVERREDGPPST"
     gene            43340..43507
                     /locus_tag="VF195_07955"
     CDS             43340..43507
                     /locus_tag="VF195_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580790.1"
                     /translation="MSKIPLGFRSYSTDVNLSEFGELFSSYEGRDLRGSPYWITTDDG
                     KVVAIEEQLIS"
     gene            complement(43553..>44746)
                     /locus_tag="VF195_07960"
     CDS             complement(43553..>44746)
                     /locus_tag="VF195_07960"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6580791.1"
                     /translation="FAEDLASSGSRVIAFAIREGPDIEPDRPPEEGFTFLGIVGLRDP
                     VRAEAASAVADAASAGIRIVMVTGDHAGTATSVSREVGLLPPDGHVLEGHQLGEADPA
                     GVSVFARVKPDDKLAIVRRFQDRGEVVAVTGDGVNDAPALRQADIGVAMGRTGSDVAR
                     EAADMVITDDDLGTIVHAVEEGRRLVRNIRNVIDYLVAGNLSEVAVVVTVLLLFPDAG
                     ATLTPLQILWINLLTDGLPAVALGVEPGSSAQTDRVDTASLLSWRRARMLGARGMSIA
                     LMSVGTFVIVRFTFDKSFESARTAMFTILVLSHLLYAFAVHLDRPAGTPAPIRSILAA
                     RGLLIAVGGGIILQLAVVAVPSLHGVFDTTSLTASGWLLCLSAALVAPIGILVVDRAR
                     SRTSR"
BASE COUNT         7472 a        15571 c        14705 g         6998 t
ORIGIN      
        1 gtgccgacgg cggcggcgat cccctgtctc gacgctcttc cctcggggtg gatctacgga
       61 ggcggcgaca tccacagcgg cgaggcgagc ttctggctga actccgatct ggccgggatg
      121 cgagcggtca cggtcacgct caggtccgcg tgcgacgtgt cgagcgccca cgaggtccct
      181 tccgatgaag ccggcgcgcg cagattcgag gctccgtcga gcctcgcgcc gcagctcctc
      241 aacgtgcgct cctacgtgat ccctggcggg tgcgccacgt accggttcgc cttcaccacg
      301 ggcgctccca cggcgttggt gttcgacgtg gacgaggccg tgtcgttcgt cccccgatcc
      361 gttctggtgg cgcacctcag ggagacagag ggtctggccc tgtgcggccg gggcgcgccg
      421 tgtccgccgt gagtcaccgg cctgtcgaac ccatggacct cgaggacgat ccagagcatc
      481 cactgctcgg gatgccgcga cgtgacacgg tggtcacggc agcgctcgcc gtggccacgg
      541 ccacgctctt cgccctcatg gccacggacg ctgggcgcga tctgatccag ccgatcgacg
      601 atgccttcct ccgctgggtg aggtcggtcc gaacggcgtg gcttacgttg atcgccaaga
      661 tcttcaacct gatcggattg acctacgtca tgacaccggt gcggatcggc gtcgcggcct
      721 tcctcgcgat ccgcagaagg tggtggcact tcatcgcgtt cgtctccgcg atcgtcgttt
      781 cggagatctc gatcggtacg ctcaaggcgc tctatgaccg acccagagcg ctcggatcgc
      841 tcgtcgagac gagtggcgcc tcgttcccct ccggacatgc cgtcgccgct tcgatcacgg
      901 cggtcgctgc ggtgatcgcg ttgttccccg aggggtcacg ccgatatcgg tggggagcgg
      961 cggccgtcgc cttctcatcg gtgatggggc tctcgcgggc ctacctggga gcacactggc
     1021 tgtccgacgc ggtcaccggg gtcctgttgg gaacatcgat cgcgctcggg accgccctga
     1081 tcgttcaccg cgtgcgcgaa cggcgagcct caggcgacgt cgcgtcgctg gtatagcgcc
     1141 atccccacca cgatcaacag agcggtccac gtgaggaaga gtgcggcacc cacgcccgga
     1201 tcgaggggcg ggccctccgt cgtgaccgcg tctgcgtgac acgcctcccc gaccgaattg
     1261 agggtcgccg ggtttggcga gagcggcgtg gctcagaccg ccccccttct cagatgtgat
     1321 tacctccgag tggagtcctc gggagggaac gatgctcgag ctgctggtct cactgctcat
     1381 caccatcctg gccgtcacgc tcgtccgacg cctgctcggc atcgagcgcg ggaggtgggt
     1441 cgtcacggtg cttgctgtgc tcgtgggaga ggtgttcgcg gtcctggtgt tgaggaccgg
     1501 ggtcggaaac atcagagacc tcccccctcg agccgcgttc ggggcgtacg ccttggtcac
     1561 ggtgttcgcg atgctcggca tcgtcgtggt agagctcatc gccaggcccg gcgcccggcg
     1621 gctgcgaggc atcccccgcc cgatccgggg aacgcgacac cttgccgggc gggccgtccg
     1681 ctacgtgaag gtcagcgtca tcgctgcgcg gcacggcctc atcaatccag ccggcagcga
     1741 tgtggacgtg cgaggctctc gtctcggcag cgcgctgagc gcgtcgttcg aggacgcggg
     1801 cggcctgttc gtgaagctcg gtcaggccat ggccgcgcag ccccatctgg tcacccgggc
     1861 cgtggccgcc gagctcgcac ggcttcagga tcgagctgcg ccagcagatc ccgcggccgc
     1921 tcgcgccgtc atcgaacagg agctcggcct accggaagag atcttcaccg acctcccttc
     1981 ggaactcctc ggctcggcgt cgatcgctca gacgtacgcg gcccgtctcc gggacgggcg
     2041 cgaggtggtg gtcaaagttc agcgaccggg ggtgcgcgag tccatcgagc aagacctcga
     2101 catcttggcc cggctcagca accgactcga gcggcgaacc acgtgggcgc gatcgctcgg
     2161 tctcaaggag ctggtcgcgg ggttcgccga gcgcacgcga gaggagctcg acttccacat
     2221 cgaggccacg cgaacagccg cgttccggcg ctcgctccag gaatcggatc gggttcacgt
     2281 gccggaggtc ttcgaagagc tctccaccgg tcgcgtcctg gtgcaggagc ggatcgttgg
     2341 acagagcgtc ggagcgcccg gcgccctcga ggggatcgac ccggaccgaa gacggggact
     2401 ggccgacggg ctgctgtcga tgacgctgcg gcagatgctc gcgggcgagc cattccacgc
     2461 ggacccccat cctggaaacg tgttcgtcga tcccgacggg cgactcgcgc tcatcgattt
     2521 cggagccatg gggcggctcg accgtttcga gcgctccggc ctcatcgaca tgatgcgtgg
     2581 gctgcagacg gaggacccat cgctcctacg ggaggcagcg ctccgcatcg gcacgcatac
     2641 aacgcggatc gacaaggagg cgctcgaccg cgagctggcc cgcctgctcg ggggggcgat
     2701 caagcccgac ggcacggtga acccggatct gttcaatgac gtcttgttcg tgttccgcga
     2761 cttcgggatc ctcctgcctc gctccacgac tacactgttc cggacgctcg taacactgct
     2821 cggaaccttg caggtgatcg cccccggcca caacatcctc gatgcagccc gtcgactggg
     2881 tggtggcgtg gtggctgagc agatggcccc agggaacctt caagagctgg tgttgcggca
     2941 ggccatggac gcagcccccg ttctcgcgcg cctcccccgc gagctggacg agctggcgcg
     3001 aacactcctg cacggggagc tgcgaacccg attgagcctc ctgtccgaac cggaggacgt
     3061 gcgcgtggca cggggcatgg cgaatcggct cgtcatgggg ctggttggct cggccctcgc
     3121 cttgtcttcg tcggtcttgc tcgtcgctcc gtcaccagtc ggacctggtc tgctcaatat
     3181 cctcggcggg atcgggatgt tcttctcggt cctcctcctc cttcgcctcg tcgttcagat
     3241 cctgcgcgag cgggactgag gaatcgccca gtccaccggc cgtcacctcg gccggaacgc
     3301 ctgatagagg tccacggggt gggtcaaacc cttgagggcc acagacccga tggcctcgaa
     3361 gcggacacct ccggtcgacc gccggaccgt ttccccactc gccagcacct cgcctccagc
     3421 ggcatgcgac gcgatcctcg acgcgaggtt caccgtccgg ccgtacacat cgccatcctg
     3481 gaagacgaca ggaccggtgt ggatgccgat atgcatgggt ggaaggtcgg caccaggagc
     3541 actctcgacc atgtccaggc cagcgagcac ggcagcctgt ggatccttga aatggaacat
     3601 gcctccgtcg cccagccagc ggatcggtcg tccgccccgc cgacgggaga tgtcttccac
     3661 gagagaggcg agctgcgacg cgagctcagc ggcggacgca tctcctcgct cctcggtcag
     3721 tctcgtgtag ccggtgagat cgacgaagca gatcgcagga ggcttcggaa ccttctcgaa
     3781 gagcccagcg tgctcgagcg cgatctccgc atggttgatc gaatgctcga tccagatgtg
     3841 ctctcggtgg cggtggtaga tgtcgaggag cgtgcgctcc agctgtgcgt tcacctcacc
     3901 accgaaccgc gctccgagat cgagcaactc ccgctcggtc cgcccagagc gccggagcgg
     3961 ctcctcgatc tcggactcgt agagttcggc ctccgccttc gtgatgcggc gcaggctgtc
     4021 cgcgtagacc cggagcaacc gaagcgtggg tccctcccgg gctccggctg cgaagaaagc
     4081 ctgcagcagg tcaacgagtt cactgtcccc tgtcttgatg cggtcggtgg gctcgggcgg
     4141 agcgaacccc aaggactcgt acagggagcg gatcgtcgca tgcggcacct cggcctctga
     4201 gcacagctgc tcgaacgtgg catcgaggcg ggccgtcgtg gtcatgacgg gggcgtcgag
     4261 ccaagagatc gataggtctc ccccgctcac gagctccatg atgccctcgg gagaaagtcc
     4321 cgcttcctcc caggcgtgga ggatgcggat acgacctacg tccgagaggg ggtagctgcc
     4381 atctcggttc gctcggaggc cgccgagctc gatcaggcgg tgcacgaagt caacgggaac
     4441 ctccgcctgg ttcgcaagtt cctcggccga gagacctcgt gaaggaccgc tcggggggag
     4501 accggacatc tcgcgaggat agtccgacgt gacgcgtcca tcggcgctcc ttccgtcgat
     4561 cagatgatca gcgcgatccc gatcgcgagg agccacacgg accatgcggc atagaggatg
     4621 gggatcgccg ctcccgcagg tgcccatcca tcctcatcgt cgcgcccgag gaactcaccc
     4681 gatcccacga tcagtccggc accgatgacg atcccgaccc atcccatcca ggcggggatc
     4741 atctgggacc cgacggccgc ggcgccggtc aggccggacc agatgccggt gaacaggtac
     4801 ccgagatgct ctccgacacc gacaccgagg tatcgatgca tcgcagagaa cacggcgatg
     4861 ttggcttccc gttgtccgat tccgagggcg agatctccgt tcatgcgagc gagcgagggg
     4921 acgaggtaga cccatcgcag cagcccgagc atctgcacga gcccggcgag gacgccgata
     4981 ctcaccgcga gcgggacgac tcccgtgaac ccaagggcct gtccgagcag tacgacggcg
     5041 gggatcaaga ggagaccgga gagcatgaac atccaccaca ggaggatcaa tgacgacccg
     5101 ccggcacgga accgatcgag gatctcggtg gcgggtctcc tgaggatgtc cgggtagtcg
     5161 aaccgctagg agctcacgaa ccaaccgagc gcccacgcgg gcgggttcgc cactgcccac
     5221 cagaacgcac ccggtacccg atgtcgcgcg aggaccagcg cctgcaccac cccgaccgcg
     5281 aggccgttca ccgcgcccat gagcacgagg tcgggccggc cgatcccgta gtcgaccagg
     5341 gccgctcccg cgacgaggcc cgccgccatc cccgcgatcg tcgcagggac ccagacccac
     5401 gagacccacc gccgcaacgc gagccactct gccacgccga cgatcgcccc cacgatgagg
     5461 cccccgccga gcgccgctcc cacggaatcg atcgcgtcca cggcgaggtc cgcgacgtag
     5521 ccaccgatcg gcaagctcac caggatcgcg aacggccaga gccagagccg gcgcgcgatc
     5581 ccccttccgt ccccagggcg atcctcggat ctcgtggcct cgttcgtgga cacggttgtc
     5641 gcgctcatgt cgtccttccc tcccccttcg ccggttcgtt cagccagcca ccggcatcgg
     5701 cggcggcggc gcaaccgcga ccgtccgctc gattcggccg gcacggatcg ccacggcgag
     5761 cagccccagt ccccaggcgc tcgcgagcag ccagagcaca cctccgagga tcgggatcag
     5821 cccgagcgct cggaggatca ccaagccgac gaggaacgcc ccgtacctcg aggaagagcg
     5881 catcaccaga cggccgaccc cgagcgtcgc caccgtgtat ccgagcgtgt agatgagacc
     5941 gaggccgagc agcacgaaga gtccgagcgg gatcccgacg accgtgacga gcaggagcac
     6001 cgcggcgacc ggcagcagga agaagaggcc tatgccccag ccgatgctcg agcccagacg
     6061 ggcctggatc gttccgacga cggcctcgtc gagacgaggt gcgaaggcaa ggagcaacag
     6121 tccgaggatc aggacggaaa ccgtataccc gagccaccag acgaagcggc cggcgaagcc
     6181 cgccatgtcg tagtcgaact tcgtcgtgat gttgctccgc gacccacgga ccgtcgcgcc
     6241 atcttcaacc gttggggtcc cctgtgtcac gagatcgccg ccgatctcgg ctccggatcg
     6301 caccgtgacg tcgccgttga acacgaccac gtctccgttc accctgccgg agacctcggt
     6361 gtccccgttc agcacgaaca ggtcctcgcg gacgatcccc tcgatcgtgg ctggaccgtt
     6421 gaagatcacc gccgtgtcca cggtgcggtc gccctcgacc agcagccggc cggtcaggac
     6481 gacttggtcg gtgtcatctg cgcccgatcc gtcctcctgg gccgatgcgg ggccggcgaa
     6541 catcaggaga aggacccccg caagcgtcag cgatcggatc agggtgtgcg acctgcctct
     6601 ggatgccatg actcctccga tgacctggag acggtgagag cagccgcctt ctggcgcgcc
     6661 tccaccggtc tccccgaatg tgctgaccac catggcgagg gggcgttacc gtgacgtgac
     6721 acacgccgcg atgagggcgc acgctgggga ggttgtcctg gatcgggagg ggaagaaggc
     6781 gtgagtcgag atagatccgg tgtcaggatc tcgctcctcg gcggtttcca gatggagtcc
     6841 gagggacccg ccttggtcct cccagaggga tcccaacgcc tcctcgcctt cctcgcgctg
     6901 aaaggcagat tgatgcaacg acaagcggtt gccgggacgc tctggccggt cgctaccgag
     6961 gtccacgcat cgtccagcct ccgatcggca ctctcgcggc tgcgaggcag agctcgtgcc
     7021 gcagtggcaa caaccgcccg ggatctcgga ttgtccgacg atgtcactgt cgatctctgg
     7081 gacgcgcggg cgctggcgca ccaactgctc acgccgacac cagcatcacc ctcggtggat
     7141 ccggggcccg aggcgatccc gacgttgtcg gcggagctcc tgccggactg gtacgacgag
     7201 tgggctctcg tcgaggccga agactggcgc cagctccgcc tccacgctct ggaggcgctc
     7261 gccgatcgtt tgtcggctca ggggaggtac ggcgacgctg cggccgcggc tctcgcggcg
     7321 gtccgggccg agccgctcag agagagcccg cgggctgcgc tcatcagggt gcatatcgcc
     7381 gagggcaacc cgaccgaggc gcttcgggag ttcgcccgat acggggagtt gatgatgctc
     7441 gaactcggcg tggagccaac cgagcgcctg cgagcgctgg tggctgatct ttgggcggtc
     7501 acgcagccgt aacggtcggt catccatcct acgggcgtga acgaacccac gggagtagca
     7561 ctcctcacga tccgggcgtg gtgcgaggaa ggttctcact cccccctccg cgctgacgtc
     7621 cggctcgcag aggatgtcgc ctctggtttc cgttcaagcg tgaccttcat cgacacggag
     7681 tcggtcctgg aggccgttcg agccttcctc gagagcgtcc tctgtccctc acggcccctg
     7741 atcctcccgt gactagcgcg acgactccac cgacatgtca tctcgggccc ggcgcggaag
     7801 cagcgccgac ccgatcagcc cggcggctgt tacgaaccag gccgccagag cgatccagaa
     7861 cgctgtcgtt gcgatcgcgg actgcaggcc cacgtggagc gccgcctcga gccggaacgt
     7921 cgaggcggca tacatcccca gcgggaagac gagtgaccaa tactgcgggt cgtagcggag
     7981 cggcacccgc tggacgccgt ggcgccagac accgatcgcg agcagcagcg ggatccacca
     8041 cgtcgcgaaa ccccaggcca gcaacgtcgt gccggtcagg aacgggacca ggccgaccga
     8101 tgtctcccac gatccggacg ccaccacgat gttcgagccg gcgagcgtcg tgatcgccag
     8161 ggcgcccatg ttgatccagt agggcggcgt cacctgctcg ccggagagcg ggaagaacgt
     8221 ccaccggtag aagatcaacc cgatcacgac gacgtagagc atcaggccga cgaggtgcgc
     8281 gaggatcgag accagtagga gaccgcgcga tccccccaga gagggcgcca acagcgctcc
     8341 caacgccgcg atcgactcgg tcgagacgac caacaggagc cacgcgccgc cgatcccgga
     8401 ctcgagcggc ggcttctccg cgcggaccgt caccgccgtc aggaaccagt acgtgatcac
     8461 gaaccagagc acgaggccgg cgatccacag cccaagggcc aggccgtccc atgacgtcag
     8521 caacgtgacc ccgctcccga ggacgttcgt ccccgcgacg atcgtgagga accccgcccc
     8581 gcgctggtgc gtcgcgatgt cgtcacgcac gcggcccggg aatcgcacca gccgcacgat
     8641 cgtgagcatc cacagcacca cgtacgcgat gacgttcacc gccaggaacc cgcgggcgag
     8701 cgtctcgaac ccctgctggt acgccgcgat cgcgacgatg ccggtcgcca tcaccagcgc
     8761 gaaggagccc gggaacagcg tcgcgatcgg tgaggtccgt tccgtcgccg cccgtgcgct
     8821 catcgatccg tcggggacga ccaccacacg gcgagcgcaa gcacgaacga caccccctgg
     8881 aaggccagga gccatcggac cgtccccatc tcctcgagga gatacgcgtc cagcgcgatc
     8941 gtgagcgccc agagctgacc gacgacgatc gtggccacga tcgcgatccg ggcgacgatg
     9001 acggcctggc gctggcggtt cgccctcggc tcgatccccg gaccgggccc gggtgcgacc
     9061 tcgaagtgcc ggcgccggtc gccgatcacg gcgtggctcc caccgcgtag atcccgtccg
     9121 accgctcttc gagctcgatc accgggagtg gccgcggcgg cggtccgtgg acgacgctcc
     9181 cgtcggtcgc gtcgaaccgg ccctcgtggc acgggcagtc gaggacctgc tcgccaggct
     9241 tcgggatcac ggcgcacgac aggtgcgtgc agatcgagga gtacgcgacg agctgccccc
     9301 catccaggcg catcgcgatc gcgcggtcct cctcggtggg gtaggcgaat cgcacccatt
     9361 cgccctcccc gagagaggcg gctaccagga ccggtgtggc cgtcccggtc cgccggggga
     9421 ataggccggc cgcgacggcg acggccccgg ccaggagccc ccccgagatc gtgacgagga
     9481 tccgcaggta gtcacgccgt gagaccagcc cctcggacgt gatcctctcg agctggcgac
     9541 gctcctcggc gacgtccgta ccggtgggct cggaccgcgt cacggagtga atcccccggg
     9601 cacggcgccg gcggcgcccg ccggcacgcc gacgggcgcc gccggcacga cgaccgccgc
     9661 gcccgtccgg acctcctcct caccgaacac gacgatgtcg ctcgctgccg cgttcggccg
     9721 agcgttcagc agctcctcga gtgtcccgta gaagatcgac cctgtcgggc acaccgacgc
     9781 gcacatgggc tggagacctt cggacgtgcg gtcgtagcag aggttgcact tgtacatcaa
     9841 gacctcgtcg gcatcgagct tcgggacgcc gaacgggcac gcgtgcacgc agttcgcgca
     9901 tccgatgcac cgctccttcg ccgcctcctg caccacgccg tccgccgtca cgaggatcgc
     9961 gtcggtcggg cagacctgcg cgcacggcgc gaccggatcc tcgcagtgca tgcagaccgt
    10021 cggcatcgag gcgacggtcg cgccctcctc caggtagtcg aggtggatca tcgacttccc
    10081 gcgatgcgag tcgcactcgc ggcacgcggc cacgcaggcc tggcagccga tgcaccggcc
    10141 ggggtcgatg aagagcgtac ggttcatcat gactcgccgg tccccctccc ctgcatcgag
    10201 gtcggcgggc ggtcgtccgc cgctgctcgc gcggcgacga cgtcgaccgc ctggcctggc
    10261 gcgagcggcg gcggcggcgc gggcgtgatc gcatcgccct gctccacccg gcacgcgcag
    10321 accttgtact cggggatctt cgagatcggg tgcagcgcgt ccaccgtcat gaggttcgcg
    10381 gacgcggcac ccgcccagtg gtacgggacg aagaccgtat cctcgcggat cgcctcggtg
    10441 accagcgccg ggtacgtcgc ctcgccccgc cgcgtgacca cccgcacggg gtcgccgttc
    10501 gcgaacccca gcgagggatg aacctcgacc cacggccgcg gcgcctgctc cacgagcggc
    10561 gcgatccggc gggtctggtt cccggacagg aagtgcgcga cggtccggcc ggtcgtgagc
    10621 cgcagcggat actcgccgtc gacgggctcg tgcggctgct gccactccac gcgattgaac
    10681 cgtgcccggc cgtccggcca ggagaagcgc tcctcgaaca gccgtggcgt gccggggtgg
    10741 tcctccgacg ggcacggcca cgagatcgcg ccctcgcgct cgagccgctc gtaggtgatc
    10801 ccgcggtagt cggcgttgcc gccctcggtc gcccagcgga gctcctcgaa gatgtcgtcg
    10861 atccccgcgt agcggaactt ctccccgacg ccgaggcgct ccgcgatctg cgacacgatc
    10921 caccagtcgc gcttggcctc gcccggcggc tcggcagcgg tgtgacgcag gacgaccttc
    10981 ccctccgcgt tcgtcgtgac ccccccgtcc tccgcccaga ccgcgctcgg gaggaccatg
    11041 tccgcgcgct cggccgtctc ggagaggaag aagtcgagct ggacgtggaa ctcgagcgcg
    11101 tcgtagttcg cggcgatcct ggtcgcgttg ggcatcgaga cgagcgggtt gttgcagatc
    11161 ccgaggagcc cgcggacctc ggtgcccatc agatggacca gctcgacggc cgaggcgccc
    11221 gtatgcggca ggtcggcctc gtcgatcccc caatagcgcg acatgaacgc ccgggccgcg
    11281 gggtcctcga tgtctcgctg gccgggcagc tggtcggtct tctgcccgtg ctcgcggccc
    11341 ccttgaccgt tgccctggcc ggtgatcgtg ccgtacccct tccccggagc gccgatctgc
    11401 ccggtcgcca gaacgaggtt gatgatcgac aacgcgttct cgacgccctg gatctgatgc
    11461 tcgatccccc gggcatggaa cgccatcgcg cgctccgccg tgccccacat ccgggcgagc
    11521 tcttcgacga gtcggggctc gatcccgcag atctcggccg cgcggtccgg gtcgtacgct
    11581 gccacggctt cccgaacgtc ggcccacccg acggtccgct cggcgatgaa cggctcgtcc
    11641 agccagccct cccgggccac cacgtggagg accccgttga agaaggcgct gtccgtcccc
    11701 ggacggaggg gcacgtacag gtccgcggtc cgcgccagcg gcgtctcgcg cggatcgatc
    11761 acgaccagct tcgcgccccg gtcgcgagcc gcccagacgt agttcgacat cacggggaag
    11821 cactccgcga cgttcgagcc cgcgacgagg atgacctggg tgtccagcat gtccgaccag
    11881 gggttcgcgg cccgatcgat cccgaaggcc ttcttgttcg ccgccgccgc cgacaccatg
    11941 cagaggcggc cgttgtagtc gatgttcttc gtccggagcg ccacgcgggc gaacttgccc
    12001 atcaggtacg tgacctcggt ggcgagcgac gaccccgagt acacggcgaa cgcgtcccgg
    12061 ccgtaggtct cctggatccg gtggatctcg gacgcgaccc gatcgagagc ctcgtcccac
    12121 gatgcccgcc ggagcggctg tccgcgcgcg tcccgcatca acgggtacag gaggcggtcc
    12181 gggtggttca cctgctggta ggccgtgacg cctttcgggc agagcttcat ccggttgatc
    12241 tcgtggtccc gaggctccat cccgaagacc cgcccgcggg cgtcgacctt caggtacatc
    12301 ccgcactgga ccccgcagta gcaacagtgc gtgggaacga gtttcgaacc cggcggcgtc
    12361 tcgagctccg tcggggccat cgggctctcg cggaagtcgc cgacaccggg cggaaggatc
    12421 gtcgacacct cagaagcctc gcttcacgtc gcggaggtac gcctgaccgc gctggacgcg
    12481 cttgcagcgg ggacacgtct cgacccacgc gcggtaccgg aggtccagct cgtccatcgt
    12541 ctcctgcagg ttgcggacga acgccgccgt ctcgagcggt tccccgcagc gccggcaccg
    12601 cgtgacgccg tcttcggtcg cggacacgtg cttgaacacc tgcacgccca ggacggccgg
    12661 ccgctggatc acgtggaaga acttcccgaa cgggatgaac acgagcgaga gcacgaccac
    12721 gcccatgtgg aggatcgcca ggaactcgta gccggcgccc ccgagcagca cgctcgagaa
    12781 cgtgagcagc agcccggtca ccgagatcgc gatcagtgcg atgagcggca cgaagtcgta
    12841 gccgaggcgc tgctccgatc gaacctcccg gtcgcggatc cggcgccaga ggaagtatcc
    12901 ggagccggcg atcaccagga cggccgccac gtcgaggaga tggaagacga tccagccgaa
    12961 ccacgtgaat ggctcgaagg tgagtacgcg gaggccccag acgtacatct ggtacgagcc
    13021 gacgtcgtcg gcccggaagt ggatccatcc ccacgacaac gcgaacgtca tcagggcggc
    13081 gaggaccaca ccccagaaga tcgcgacatg tgcgagccat cgtcccctgc tgcgctcccg
    13141 gaggaacgtc tggaagccga gatacgaaac aacctgtttc gggaccagtg tcgggaaccg
    13201 ccggaagttc cgccacgaga ggaaggcgcg ccagcccttc aggaagtagc ggcgggtcgg
    13261 cggctgctgg atccagatcg tgtagcggta gacgacaccg aagaccagga cgagggtggc
    13321 cgtcgcgtag ccgatcagcg ctgcgtcgaa tgtccgcaga cgtccgctcc cgaagtacac
    13381 ggccatcgcg acgagaccag cggtcacgat gccagcgaca gaagctcgcc gctcggcttg
    13441 catcagcgac ggaggccgcc tgtcgtccat cgccacagag cctatcgact ggaggctggg
    13501 gagcccgctc ggccggacag gcccgaacgt catcacgcga cggtcccccg ttgcatcggt
    13561 gggctctcag atccggcacg ccgagactgc ccaacggccg tgtcccggcg cgtgatccag
    13621 gggccgtgcg tggcgctcga gggagcacag cgagtccctc gagcgcctga ggccggcctc
    13681 atgcgacgag ctccttcggg cgagggagcc cttcgaccgt cgtgatacga ccggcgcgtc
    13741 ccggctcctg actcgccttc gagggaggca tctcgtcgcc gcgaagcgtc agctgtccgt
    13801 gcaggacgat gtgcgcgaga accggggcca gcacgttccc ggtcaggagg aatgcgagcg
    13861 cctgtatgcc gcacgcagcc agcgccccgg ccagcatctt cctcgaacgg cggaattcgc
    13921 gataccccag gtggtggacg gcgatcacga tcagcgcacc gacgatcgcg agcgcccccg
    13981 acgcgatcgc tccgacctcg agatccgtcc atcccagggc ctcagcgcct tgccacacgg
    14041 cgatgaccgg gagcgtggcg aggagcagac cctctgccat cccgtacacc actccttccc
    14101 agaggagccg cccgaccaac cgaggaccct caggatgtgg cctcgagggc agccggcgaa
    14161 cgagcgggac gaccacggcc gccgccgcga ttcctcccac gatcccccag ccccactcgt
    14221 cggtgaggag atcggcgacc tcggtccccg accaggccac gtagcccaca ccgagcacga
    14281 gggtcgtcac ggtcagaacg gcgacgtacg gcgttctcgg gacgcgagcc agatccgtca
    14341 cgatccatgt caccgcgaac gcgaccaccg agaggacacc gaaggcccct gcggcttcca
    14401 accacccgga cgatgcatca gcaactggca tcagactcat ctccttctcc gaggccagca
    14461 tccatctcga ctcgccaccg aaagtaggtc tcgtggcccc ctcatcgagg gaccgatgtc
    14521 cacgagagcg gtgcttcggt cagcgagcgg cgatggagag cggttcatca cacgtcgatc
    14581 ggggacttcg tccggggatc cggagctcga tagtggacgc gccgacgcac acgagtgtcg
    14641 tcgggcccgt tccccgaccc cacacgaagc ctcttgacag ggcacgatcg cagagcgccg
    14701 tcgatggaga tcaagcgatg cggtcgtgcg tccgggacgg gcgcgaacgg acgatggacc
    14761 tcgcaaccaa cagggccacc aaggtgacga gctcggcccc gaggatcact cggttgaagt
    14821 cgaccgcggg acgccacgag acttcgtctc ccttgatcac aaaggcgccc accggcttcg
    14881 cgctgaggcc gaagccagca cctcctccac gtccttcccc gttcgaacct tcgcccgagc
    14941 cgccaccggc gccgccttgg acgcgggcag ccgggatcag cgtcacgccg ttcttcacat
    15001 acggctcgcc gaagacgcgg cggacggtga tggcgtcctt cgcatcttcg atcgtcttgt
    15061 cgacgtcgac cttcttcgtc gtttccatgg tgactccttc ctcctgtcca tcccagcatt
    15121 ccacctccag gatccgccgg ctcaggccac aggtccctga gaccacggtg ctgtctgcca
    15181 cctcaactcc gccgtcgatg tggcaggcac ctcccgcacg ccgacacggc gcaacgtgga
    15241 aggagcccgc ctgccggcgg gctcctcccg tgaagcggcc cggagctgtg gccgctcgcc
    15301 ctagcgtgtc gagccggcgt actccgggtc gttcacccgg tgctccgtcg ttgtcgccac
    15361 cgccctggct gcgtgccgga agccgaggat cgagaagatc ccgagcaaca gacccgccgc
    15421 caccatcgcg aaccccgccc acagagcgat cgtcgcgacg gtccaccagc cgtaggcgtt
    15481 cagcaggatc gccctcaggg tttcgccctt gaagagcgtg tctgccttcc cctgcagctc
    15541 cgcggcaacg tcggggtcga gcccctcttc gcgcgccgcc gagctcgttt ccgagtaggt
    15601 cgccccgtcg ttgatgccca cgaggtgtcc gccgatgtag cgggagaacg cctccgcctc
    15661 cgcgccggtg tcgaccttct cgccggcgaa ctcgccgaga tctgccttct cctccggcga
    15721 catcgcctca gccggcggga acacgatgtt ctgtggctcc aaccgatccc gcacgttctg
    15781 ctggccgaag ttgccaccgt agatcgccat cgcgccgagc gcgatgagca cgaccgcgat
    15841 cacggcacct gcgccgctca cgatcttgtc ccagctgctc ctgtccatct catcgctcct
    15901 tcgctcctgg gggccgccgc ttccgtgggg gcccgttgtg ctcatgttcg acccccagcg
    15961 cctcccttcc atgggccttc ggaccccaca ccgggtggga cctgcccagg atcaccaagg
    16021 accccgaagg acgcctcacc ctcggttccg gagatcgtga cggcatccac cctgacgaga
    16081 gagcttcgag atcctcctcg atcgacgggt tcatgccgct tccgcgacgg tcgcgggacc
    16141 atggtcaccg aggaactctt cgaggaactc ggcgactccg tatcgcgtcg cagagtgcct
    16201 ccctgcacga ccatcgtggg ctccgggacc ccggacagtc gatctggttg atggcgtgat
    16261 ccgacaggat catctccccc tctcctaccg ctcgctcacg cgccgaccat ggtctcggac
    16321 ggccatgtga gccacacgat cccgacgatc agggccgcgt tgatcccctc gatgaacagg
    16381 taccaagggt tgaagtacac gaccatcaga ccgaacgaag ccgccagccc gatcgcagcc
    16441 acgccacgcc accagccggc gtgcgcccag aggccgagac ccccagccat gagcaacacg
    16501 gccgctgtga cggcaaggag aaccgccacg gccctctggt caccgagcaa ccaggagtgg
    16561 ttcgggtcga acggtgcctt ctggtcaggg ggcttcggca ggagccagat cgccagatgc
    16621 acgagcgcgt gcgcgaccag gaatgcgatg aacaggaacc gccacatcat cctcacctca
    16681 cgtggaagag aactgctctc cttcggcctt cgccagaggc ggcgtgccct cccactcgac
    16741 aacggtctgc acaccacgtc gctccagctg ctcggcgagc gctcggacgc cggcaaggca
    16801 gtcgtcgtct ggatagcagg gagcgaccac ctcagctcgc tcgacccctt cgtcgagctc
    16861 gaagtccccg gcgctccaca ccgcggccca gtccggctcg aagtcgggca gcacgacccg
    16921 gagatacggt ccattcgtct gaacgaccaa gtcctcagac atctccgcct cctcctcacc
    16981 cgtcacatca aggatgcgcg acgcggcaca cacggggaga ggcccaacga caccggttcg
    17041 acgggacaga ggtcccgatg ggcgaggtga ggtcgcggac gagcagagcc cctcgatcga
    17101 ggtctgagac ccacgacggc ggggtcgagg cgaggtggcc actcgtgtcg aacggcgctc
    17161 tcgacgggac gagtggtact gcggcgccga cccttcccct ccgatgggtc gccacgcaag
    17221 taagggggcc aacgatggtg tacgcatcgc tcttcgggag gctgttcaag atcgacctcg
    17281 tcgccccgga ggtcccccgc tcgtcggtgg ctcatcgacg ggtggtggat cacgccctca
    17341 cgaacgagga gatcctcttc gacacgggca cccagccgtt ctcgaagcag ctcgccgacg
    17401 ccgtggaaac gttctggcgg gtcggtgtcg ccgcccagag ccgaaccagc agacccgacc
    17461 gtggatgttg gctcctatcg cccccgcgcg gagcgatgtc gcaggagatt ccttccgtgg
    17521 aggtacgcag tcaagaggat gagcgggacg tggatcgcgg gcagggcatg ggcgaaccag
    17581 cgcggcagga agatgtcggt cacggaaccg tcttcggccc cgagcgattc ggcaacatcg
    17641 gtcagcggac agcggaagcc gttcgcggcg aagatcagac tctcgccgcc gaccacggcg
    17701 gccgcgaccg cggcgcgccg gtcggtcgct tcgcgaaccc agcccacagc aggtacacca
    17761 tgcacgactc gatggagaac caggcgagcg tatggatggc cttgaccgcc acaagtgcac
    17821 ggccggcact catcgcatcc cccttgaccg cgcttcgagg acgcgcttga ggctggccca
    17881 gatggtctgc tcctgacgcc gacctatgcg agtgatcagc ggcgtcatca gccggagggc
    17941 gccgcgcggc ttcaggtgcc atagccaccg catccgggtt ccgtcggcaa ggggttcgaa
    18001 gcgcaggtcc ccctcgatat ccatgttcgc cagatgtgtc ctcacggcga gtcgccgggg
    18061 tcgctcgtac ccggtgttct tgatggtcat cttgaccgtt cgaccggccg acctcatctc
    18121 cgcgctgaac cgcgttccca gcccgaccgg cccgggcgag gtttgctccg cgtgaagcat
    18181 tcgggggttg tagaggggct cgttccgctc gtcggcaacg aagtcgaaga cctcctcaac
    18241 cggccgatgg atgacgatct caccttccac tcgatcgttc atcatcgaac cccctcgggg
    18301 cgtggtcggc cggtctcgtc aaatgagcca gggatcacca tcgatcttca ccgacactcc
    18361 cgtcagggtc catcgtcgct cttcctgcgg gccctagcgc cgctggtcat cggctcgagg
    18421 cggtttccag ttcgcggacg ttgcgggcag tgctggccag tcccgtccca gctactcgcc
    18481 ggtccccgga cttgccccaa gctccttggc gattccgtcg gcccagtcgc ggatcgcggc
    18541 ccagtcgcgg aagtcgccct cgggcgcctt caccgccttc accatcatcc gttctccgaa
    18601 cccaagcttg ctccggtcga gagcgccgaa gaacatgcgg tgatcgaccg ggccgatgat
    18661 cccgcccatc tctcccaact gccgaggctg ttcctcctcg tcctcgacgt ctgcgccaag
    18721 ggggccgcta ctgaagagcc atacgggcct ctccgcgagc gcctcggcga agcggtgaac
    18781 gaactcgacc gcctccttca tccacgaccc caagtagaca gcgctcccga gtacgaccgc
    18841 tctgaactcc gcaaggtcgc cgacctcatc gacggatccg acttccacgg gctcccccag
    18901 ctcgcgcaag cgatccgcga tcgcgtgcgc gatcccttcc gtggagccgt gcttgctcgc
    18961 gtacgccacg agcaccgtca tgccaaccac tccttccgtc tcagatcctc cgaagcccgt
    19021 ccatacaact ctaggactga tcgaggaccc gctcgtccgc cttgcgatca agcaggagcg
    19081 tcagcgtcgc cagcgcgaaa ccggacgcca ggacgatgcc ctccaggggg aatgccttga
    19141 cgcctggcac atcggcgttc gcctggaaca cacccgccat caagagattc acctggtaca
    19201 tcgcacccat gacggccatc gcagtgccga acaccaagcc ttggggtgtc cgcctccaga
    19261 ggaggatccc gctgatcacg agcgccggca cgaggagcga cgtgtcgatc gcgaacacga
    19321 gatggatccc gtcggccgga agatcggcca tgagctcacc ggtcatcgcg aaacggatcg
    19381 ccaggaagag ccacagcccg cccagcgcga tgccgaccac ggccaggaac gcgccgatca
    19441 cccctacgcc cgtcacgtcg cggaagcggg ccgcgatcgc tcccacgtcg atgttcgcga
    19501 ccgccaggac gagcgcagcg atcgagagcg agaacagggc gatgtggatg acgaagatgt
    19561 cgttgaacgc cgcgccgaac acgtagtacg cgtagttgta cagcgaatac gcgagcgcgc
    19621 cgagccagac cgcctgagcg gcgcgggagc cgcgcctcgc gagcagcacg gatgcgacaa
    19681 ggatgggggc ggccaccgcg atcgtcgtca ggtccccccc tcgcagcgct ccccgagccc
    19741 agggaccgtc tcgatacaac ccgtcgacga gcagaccggt cgccgaggcg accaccatca
    19801 ggaccccgat cgcaccggag acccagtagg cggcgcgaag accaggtttc gctcccggtc
    19861 gacgcactgg atccagtgca gcatcctgca cgttgtgccg tctcgactct cgaaccagct
    19921 ccgccgccat cgttctcacc tctcgtcatc cgtgacaacg cgaacgtagg gtccggactc
    19981 acgacgacca gagggcaacc gtcccgcttc gggcgggacc atcgcctgtt cgcgacgacc
    20041 gcccgactcg tacctgtcat catccgcagg cctggaggga tgccgtgtga gggaagtgga
    20101 gctcgagacc cgatcgctcg accagctcga ggggctggtc cggccctctc atctcgagaa
    20161 gttccgatcg cttctcggca cggccgccga tcgactcgaa ggccgaacgc tctggcacgt
    20221 gaactcgacc tctgatggcg gaggagtcgc cgagcttctc catcagctcc tcggctacct
    20281 gatcgaagga gggatccggt gtcggtggat cgtgatcgaa ggtgaccagg acttcttctg
    20341 gatgacgaag cggctccaca accggctgca cggatccggg ggtgacggcg gccccctcgg
    20401 tgaggaggag cgggagatct acatgcgcgt gcttcgtgag cagctctcgc cgctgctcga
    20461 tctcatgcgc cgaggcgacg tcgcggtcct gcacgacccc cagaccgcgg gcctcatcgg
    20521 cccgctggtg gatgcgggtc tcggcgttga gtgggtctgt cacgtcgggg tcgacatgcc
    20581 gacagatatc gtccgctccg cctgggcgtt ccttcgcccc gacgtcgaac aggcgcacgc
    20641 ctcggtgttc tcacggcgcg cgtatatctg ggaatggctc gatcggcgga aggtctccgt
    20701 gatcccaccc tgcatcgatc cgaggtcgcc gaagaatgcc gacctcgaac ccacggatgt
    20761 ggatgccatc ctcggtgcgg ctggcatcct cacgatgccg gaaccgacgg aggctcttgt
    20821 cgagatcgac ggcgtcggct cggttcaggt gcatcgggac tccgagaggg tcgaggaggc
    20881 accggccccc gcggatgcgc ccctcgtctc tcaggtgtcc cgctgggacc gattgaaaga
    20941 tcctgttggc gtcctgcacg gattcgcgcg atatgtgcgg ccgaacctcg gcgctcacct
    21001 cttgttggcc gggccggcga tggcgtcggt ggcagacgac ccggaaggcg cagaggtctt
    21061 cgccgaagcc ctcgcagcgt ggcagcggct tccagccgaa ccgcgcgccc ggatccacct
    21121 cgtgaacatc ccggccgagg accgggtcga gaacgcggtc gtggtgaacg cattgcaacg
    21181 caaggtcgac gtggtggtgc agaagagtct cgccgagggc ttcggcctca cggtgaccga
    21241 ggcgatgtgg aaggaacggg cgatcgtcgc cagtcgcgtg ggggggatcc aggaccagat
    21301 cacggatggc cgaagtggtc tcttgatcgc accgacagac ctgcccgccc tcgggaaggc
    21361 gatctcatcg ctcatcgccg atctcgggtt cgcccaagcc gttggccatg cggctcgtca
    21421 gcgggtcagg agccgtttcc tgccgccgca cttcctcggc ggtcacctcg acgtgatcga
    21481 tcggatcacg cgcggggcct tggagtaggc gacgccctcc gttcagatga acagcgtgat
    21541 cgcgtcttgt gcctcgagca gagccgtcgt ggcgcccgcc ggctcctcca ggccgtcgat
    21601 caggtcgtca cgatgcagac ccagcagatc catcgtcatc tgacacggca cgagcttcac
    21661 cccgagatcc ttcgcaaggt cgatcagctc ctccggactc gcgacatggt gatccctggc
    21721 gagcttccgc atcatcgcgg ggcccgcgcc ggcgaagttc atcttcccca acttcacgtg
    21781 ctcgagaccg ggccggtcca tcgtggagag cagcttctgc atccagttgt cgccggtgat
    21841 ccgcacgtcg tccttcacga gcgggaagag cccccagaag gtgaagaaga tggtggtctc
    21901 catccccatc gcggccccag tggtgccgag gatcaaggtc ggccacacac gatcgagatc
    21961 gccgctccag gccatgatca ccagtccgcg atgcgggacg tggcgggcct ctcgttcggt
    22021 cgcctctcgg acggcttccg ccgttgctgc catctcactc accgcttcct gatcaagaac
    22081 cggaatacac cgtcgtccac cgaacgctcg aggagctcgt ggccggtcga gccggaccac
    22141 gccgcgaaat cgggcacgga cccgggatcc gtcgccagaa cctcgagcag atcaccgacg
    22201 gcgagctccc gtatcgcttg tgccgtcttc gcgatcggca gcgggcaggc gaaccccttc
    22261 atgtccagcg tcttcacgat cgtcgcgctc atcgcttact ccttccctcg catcgcccac
    22321 tcggggatcc ggccctgggg caccgtttcc caatacaggc ggttgaacag ccacttcgcg
    22381 gcgtgccaca tccgatgcgg ctgtggcgga accggcggct gctcgtagtc gaaccgcagc
    22441 gtggtcgcgc ggccgtcgcc ggtctccagg aagcacatga ctcggccgcc gtagttcgat
    22501 cgcggcgccg tgcctcgaac cagagacgcg atgcggctgg ccaccacggg tgcttcgaaa
    22561 tgcgccgtgg acccgctctt cgagatcggg aggtcggttc catccccgat ggcgaagatc
    22621 cgttcttggt ccttcacctg gagcgtgtgg cggtctgtag ggacccaccc tcccgggtcg
    22681 cccagatggg acgcgtcgat cgctacctgt ccccgatgcg gtggcacgag cacgagcagg
    22741 tcgtactcgg ccttgtcccc ttcgagcgag ctcacgaccc cggccgaggc atccaccgat
    22801 tcgacgttga agaacgtctc gagctcgatc ccccgtgcgg acatgatcgg ttggatcagc
    22861 ttcgaggcac tctcgatcgt gaacgcccgg ttcagcgggg aaaggagggt gatctcgctc
    22921 cgctcccgca ggccgcgccg ccgcaggaac cggtcgagca tgaacacgaa ctccaccggc
    22981 gccggagggc acttgtaggg gatcccggcc acacccacct tgacccgccc tccgcggaaa
    23041 cgtcgcagct cctcgcggag gcgctcggcg tcctccagcg agtagaagcc gaacgctccc
    23101 tcggccagtc cggggatctg ctccgggacc agccgcgcgc cggtcgcgat caccaggtag
    23161 tcccagtcca ggctgccgcc ccgttccagc tggacgctgc ccgcgtccgg atggatccgg
    23221 accgcccgct cgatcgcgag atccacgtca cgccggagca acgtccgctc gtcgcgcgcg
    23281 agccatcgtc cgttcgcctc gcccagcgcc aggtagagga agccgggctg atagacgtgc
    23341 atgccggtcg gatcgaccac cgtgacccgc gcgtcccggc cgagctcctt cgagagcagg
    23401 ttcgccgcca gggtaccccc gaccccgccg ccgagcacca cgatcctcgc cggcatcggg
    23461 ttacctcatc ttccgcacga cgaagctggt cgcgccgtcc gtctcgacca tggagacgag
    23521 ctcgtggctc gccttcttca cccatgccgg gatgtccgtc agtgagcctg catcggtcga
    23581 gaggacttcg atctcatcgc cgatcgatcc ctcccggatc gctccgatca gactcatgag
    23641 cggtcccgga cagggcatcc cccgagcgtc gatggaccgg gtcacgtcgg tccctgtgtc
    23701 atcgctgcca cctccgtctc ctcacacgaa cgtgatcgca ccgccggccg cggccaggaa
    23761 cgaggccacg ccgcgtacgt catccaccag cggatccagg tcgtccggag tgatcctcag
    23821 caggtccatc gagagcgagc acgcgtggat cgacacgtcg ccgatctcct tcgcctcccg
    23881 gagcatctcg ctccaatgcg tgccctcgtg ccactcccag agcgtctgtc cgtctgtccc
    23941 tgcctcgggc gacagtccat ggtccttcat gatgcgatcg gcccggaagg cgtcgagcgc
    24001 ccagaactgc agcaggatct ccaccttgcg ctccatcgcg gcagccccga cgaccagcac
    24061 cgcagcccca gtcagccggt cgtcggtccc ggagcagagc accaaggaga gcctgtcttc
    24121 atccgtgtcc atcgcccacc tccttcgctc cccggagcat gagccgacgg gagaggcggc
    24181 gcgggggccg aacgaccggg cgatcgggtg acgttcggcc ccatggctga ggcacagacg
    24241 gcgacgacca accctcccga gcgtcctgcg ggaggtcagg cccgctgaag gcgtcaccgc
    24301 ggcgatcgga gctagggggc cgcgaccctg acgcgtgagt ccacaaccaa tgcccgcgtc
    24361 tcccccacca tcacgggatt gcagtccatc tcccggacgg gttcgtgatc agcggcgaga
    24421 gcgctgacac ggaggatgat ctcctcgaga gcgagcacgt tcgcgcgcgg cgctccccga
    24481 tagccgtcca gcaacgggaa cgtcttcagc gaacgaacca tctcccgagc gcccgtatcc
    24541 gtgaccggtg caacgcgaac ggcgacatcc ttcagaagtt ccgcggtcgt gccacccgcg
    24601 ccgcacgcaa cgatcggccc gaagacgtcg tcatgcgtga caccgaccag catctcgaca
    24661 ccttcgacca tctcctggag ggagaagccc ttcagcggtc ccccgagcga tccgagacgt
    24721 tcctgcatct cctccgctgc cgcggttaca gcgtcctttc cgctcagccc cagccgaacg
    24781 ccgccaacgt ccgtcttgtg caacgggccg acagccttga gcgccaccgg gaccccgagc
    24841 tcaacggcgt agcgtcccgc ctcctccggt gtgatcgcca acctcgacat ggcgaccggg
    24901 atgccgtagc aggcgagcag tccgtcgacc tcctcgggct ccagccagcc gctccctctg
    24961 gagagggccg cggccaagat ggcatcggct tcgtccgaca ggacgtcgat caagcggggc
    25021 acggtgccgg ggctgcggtc gcgccatcgt ccgtactcga cggcgcgcgc ggccgcgatc
    25081 gccgcctgct cggggaatgc gaaagagggg aaccatctcc ggccgctcac gggatcgacc
    25141 gggacctcgc ctcccgacat gaagctcgtg agcacgggcg tgctcccgcc gacgctcgcg
    25201 atggcctccc cgatcccccg agcgacgtcg tcggcgtgcc gctcgagagg cgggatgtag
    25261 atcacgatcg cgatatcgac actcggatcg cccgcgacgg cgatcagggt ccgctcgtag
    25321 tcctcgccgg tcgcggaggc gatcatgtcg acggggttgc cggtcgaggc ctctgacggc
    25381 aggaaggtgc ggagctcatc cacggtctcc tgggagagag ggggcagcac gagcccgttc
    25441 gcctcacagg tgtcggcgca gaggatcccc agccccccgg cgttcgtgac gatccccaca
    25501 cggttgcccg cgggtggcgg ttgcgtggcc agcagcatcg ccacgtcgaa catctcggcg
    25561 agcgtgtctg tgcggatcac cccgttctct cgaaagagcg cgtcgaccgt gaggtccgac
    25621 gtggcgacga gcgatccggt gtgcgatccc gcggcgcgct ggcccgcctg cgtacgcccg
    25681 ctcttcacgg cgacgatcgg cgtgcgcttg cctatccgac gcgcgagatg cgagaagcgg
    25741 cgcgggttcc cgaaggattc gagatacagg aggatgacgt tggtacgcgc gtcatcttcc
    25801 cagtagctga gcatgtcgtt gcccgagatg tcggctttgt tcccgacgga gacgaacgag
    25861 gatagaccga tgtccctccg gcgcgtctca ctcatcacgg cgaggcctac ggctccgctc
    25921 tgcgacagga accccaccga gccggcctcc ggagcgatgg tggcgaacgt tccgttcagc
    25981 gcgaccctcg gatcggtgtt gaccaccccc atgcaattcg gccccacgag ccgcatgccc
    26041 catcggcggc agatctcgac gagctcacgc tgtctcgccg ggccatcgcc gccggtctct
    26101 gcgaacccgg ccgagatcac gacgaggcca cgtacacccc gccgaccgca tgcttcagcc
    26161 acttccgcca cgagcgaagc cttgacggcg atgaacgcga cgtcgaccgg gccggggacg
    26221 tcctcgatcg tcgcgtacga cttgacgcct tggaccgccg gagccgacgg attcacgggg
    26281 tacaccgtgc cgtggaaggg gtggctgagc aggttccgaa ggaggcgccc gccgatgctc
    26341 gtctcgtcac gggacgcacc gatgaccgcc acggtctcgg gctcaaggat cgtgcggacc
    26401 gcgctggccg cggccaccgc ctcacgctcc tcgaagcgcg tgagcgcttc ctccgttggt
    26461 gcagtggaga tctcgacaga cacggcaccg ggcttcgcgc ggatcgagat ggggaagccg
    26521 ctctggcgga acacatcgat catccggagg ttctcgggga ggaccttggc ttcgagcacg
    26581 ctgatccccg ctccgtacgc ggcgtcgacc aggttgccga ccagaagtga tccgagaccc
    26641 gacccttgga gttcgtcgga aacactcacg ctgagctctg ccctcgtcgc gttctcccgg
    26701 acgaactgtg cgccccccac gacgtgcccg gcgtcgctac ccgtgaccgc gagcagcgtg
    26761 aggtgatccg ggtattcggg cctcaccgcc ttggcggcga ggtccgtgac atcgatcgac
    26821 gccgaccaga accgcagtct cctgctctcg tccgagagtc cgatcaagta gtcctcgacg
    26881 actccccgat cctctgacct ggcgggtctg atatggacgg tccggccgtc acggagaacc
    26941 acgtccttct cgaggtccgt cggggtctcc gcatcgccca tcgcagcgaa ccgtatctgg
    27001 gagccgggca ccgtgtcagg ggaacgcgtc cgactcccca cgtcggggag ccggacgcgc
    27061 cccaaccttg ccctcaggga acgacgatca ccggcttctc ggacaggtgc agcacgcggt
    27121 gtgtgacact gcccaggaac aggccgccga agtcggaccg gcctcgggtg cccatgacga
    27181 tcgcatcgac gtccgcggaa gccgcggact cgacgatccc acgaggcatg tgtccttcga
    27241 ggacacggac gacctcgccg cgagcgttca gccctctgtc cttgaggtcc cgcacgacct
    27301 catcgacgac ctccgtggcc gcgtcgggtt cctcgaggta gctcgtcccg taacgagaca
    27361 cgatccgggg aaccgcgtgg agcacgatga tctccgaacc gtgatcgctc gccagcccgg
    27421 ctgcgaactc gacggctcgc ttgctgggct ccgacgaatc ggtcgccacc aatatccgct
    27481 cgagcatggt ggatcacctc cttcgggatc agcctcctct ccgggacgtc atccgacgag
    27541 ggaccaacgg cccgctctcg gcgggcctct cccggcgatg gacatcggcg atggacatca
    27601 aggacgttct ccgtgaggag cacgctcacc cgatcgggga ccggtcgacc gatgggcatg
    27661 acgcccgtac cgcggggatg cgctcgggcg ggtcggctat cctcgccccg tgctcgacgg
    27721 cagcaccaaa gacggggatc ggcgcttgat cgaggcgggg atgatcctcg cctccgaact
    27781 ctcactcgac gccgtgctcc ttcgcatcgt cgagctcgcg gtcgatctca cgggcgctcg
    27841 ctacggcgcc ttgggggtcc tcacgcccga gggccgatcg atcgaggagt tcatcaccgt
    27901 tgggatcacg cccgaggaac gagccgctct cggtgaccct ccgaccggcc acggcctcct
    27961 gggggcgctc atcacggagg gacgacctct gcggatcccc gacatcagtg ccgacccgcg
    28021 ctcggtcgga ttcccgccga accaccctcc gatgaagtct ctcctcggag ctccgatcac
    28081 cgggcgaggg aaggtattcg ggaacatcta tctcaccgat aaacagggcg ccgagacatt
    28141 cgatgaagaa gacgaacggg tcctcatcgt cttggcgacc caggccgcga tcgccgtcga
    28201 gaacgcccgc ctctacgacg agaccgaggg caagagccgg gagctccagc ggctccaggt
    28261 ggtggaggag cgggagcgga tcggcaagga gctgcacgac ggcgtcatcc agtcgctctt
    28321 cgcggtcggg atgagtctcc aggggttggc atcttcgtcg agcgacgcgg ataccgctcg
    28381 tcgcctggaa gcggccgtcg aggatgtcga ccacgcgatc cgtgaccttc ggaactacat
    28441 cttcggtctc cgccccggga tcctcgcgga ccgtcagctg gatcaggccc tcaaggagat
    28501 ggcgagcgag ttcggcgcgc gctccggtgt ggtgacggtg gtcgatgtcg acagcttcgc
    28561 cgcgtcggcg ctcgcctccc atgcagccga cgtggtgcag ctcgcacggg aggccctctc
    28621 gaacgtcagc cggcacgggg ccgcaacgac gtgccgcatc gctctcctgc gtggggagtc
    28681 cggcctcttc ctcgagatcg acgacgacgg gcgaggcttc gatgtgggtg tcacctcctg
    28741 gggcatgggc ctcaggaacc ttcgggaacg ggtcgagtcc ctcggcgggg tgctccaagt
    28801 cgacagcact ccgggagaag ggacgaccgt ccgcgcgacg ttcccttcct gaccaaggtc
    28861 ccgccggacc atggaccatc ggcccctccg ccgcgatccg aacccgggca ccctggtgcc
    28921 atgttcgcca ggtatttcgt cgagctgccg atgtccgcgg aggacatcga gcgagcactg
    28981 tctcgtgacc cgcgcgtgtg gctccccggg ttggccgcgc gtgcccatca ccggggcgac
    29041 ctgctcctcg ccgaggtggg gttcggagaa gcggtccggg tcaagaggac ggtggccgtc
    29101 gagctcggac agccggtccg ttccggatcc aagacggtgt tcccactccg gtgggcggcc
    29161 tccggtccgg gggggctctt cccgtcgctg gatgccgacc tcgaggtggc aacgctccga
    29221 cccggacaca cccagctcgc gatgagtgcc aggtacgttc cccccttcgg caccgtcggc
    29281 cgggtcatcg accgcgcggc cctctcaagg gtcgccgagg cgacgctcaa ggacttcctc
    29341 gaccgtgtcg ccgacgccat catgggatcc ggggacgtgc cggtccctgt cgagctgggc
    29401 gggaccgcgc ccgcatcggc gtgagcgaag cgggctaaca tcccccgatg gccaagccga
    29461 agtcccctcc gctccgcgtc atgctcgtgg acgaccacga ggtcgtgcgg gatgggatcc
    29521 gagcgatgct cgcatccgaa gacgacatcc acgtgaccac ggaagccgga accgtcagag
    29581 aggcgatcga tgaggccgac cggacccggc cggatgtcgt ggtgatggat gttcggctcg
    29641 ccgacgggag cgggatcgag gccacgcgtg agatccgcgc gaaccatccg gcgacgcggg
    29701 tcctgatgct cacgtccttc gcagatgatg aggcgctgtt cgcgtcgatc atggcaggtg
    29761 cctccgggta cgtgctcaaa caggtccgtt ccggcgagct cgtccgagcg atccgtgcgg
    29821 tgggggccgg ggagagtctc ctggacccag cgatgacgac atccgtcctc gaccggcttc
    29881 ggaaaggcaa gcatctgatg cgggacgaga ggctctcgcg cctctctcca caggaagaac
    29941 ggatcctgac gtcgatcgca gagggcaaga cgaaccggga gatcggcgat gaactcaccc
    30001 tcgccgagaa gacggtgaag aactacgtat catcgatcct ctcgaagctc gaggtcgcca
    30061 ggcgcgcgga agctgccgcc tacctggctc gccacacgac catgcccggg tcagtcgagt
    30121 aacccgtcgc cgtctccgac aggacgccgg aggacgaacc cgtctgcctc ggccgcgacc
    30181 cggaccgccg cgacgaccgt ctccgctccg ggacggcgat cgaggcggat catacggcct
    30241 cgtgccaccg ggtccagagg cgccgcgtcg atcgccacga cggtgcgacc gctcccggtg
    30301 tacagggcca gcagttcgtc cgacgacgtt ccgacgccca gctcgtcccc cgccaaccac
    30361 ggatccagga ccacgacgtc ggcccgctcc agcagcggac agtagccctc tcgcccgccg
    30421 atgcatgtgt agtccggagc cgtcggaccg gggcacgcga tcacctggta cccggcctgc
    30481 tcgagagcca tccccagccg ctcccgctct gcgtcgtctg actccaccaa caacacggtc
    30541 ccctcgtgcg gcatggcaca cctcctcctt gccggtgagt ctccgctcac cgcccagagg
    30601 cggaacgtgc cgaaggtccc ggtcgaacag agtcggtcga ccgtaggggg gggatgcacg
    30661 aaccgggatg ctgctcaggt gaagctgatc aaggaggtgg gacagatgga gacgatcgag
    30721 agaagggctc tcgacatccc ggcgttggag cggctcgagc cgatccacac caaggggctg
    30781 cagcgcgtgt tcggtgccct ccggctcgtc gccggatgga cgttcctgtg ggcgttcctc
    30841 gacaaggcct tcgccctcgg attctcgacc gggcggatcc tgaatgacgc cgggcagacc
    30901 gtgaagatcg atttcttcgg accggaggcc tggatcaacg gcgccagccc gacggcagga
    30961 gccgtcggtt tcggcctgaa gggtccgttc gccgacaaca tccagagcat cacgaacttc
    31021 cagatgacgc aggccggccc tcaggtggcc ggatggctcg actgggtgta catgggctcg
    31081 atgctgctga tcggcctcgg cctcatgctc ggggtcggaa cgcggctcgc cgcactgggc
    31141 ggcatcgcgt ggatgacgat cttctacgcc ggcacggcga tctggccgga gcacaacccg
    31201 gtcgtggacg accacgtcgt gatcgcggtc gtgttggtcg gcctgatgct ggcgaacgcc
    31261 ggacgctact acggcctcgg caaggtctgg cagcgggtcg ggttcgtgaa ggatcggaag
    31321 tacctgtact gatcgatcca aggcgagacg ggcggggccg aggccccgcc ttttcgcgtc
    31381 ggtggacctg tccggacggt cccccaacga cgggtctttc gacccatcgc gaccgatccc
    31441 cgaggttcca ggctgtccgc acgagaggaa gcgacgcgag cgagagcgac gcgctggcga
    31501 aacggagggc ctgagatgcg cccggcttcg gagggtcacc ccttccacgg ccccgcccga
    31561 tcaggcgccg cgagatccga gggaggtagg aacatgacac gcgcgctggt tgtcttcgag
    31621 tccatgttcg gcaacacgca gcagatcgca gaggcggtga aggagggact ctcctcgtac
    31681 gtgttggccg acatcctcga ggtcggtacg gcacccgacg tccttccgaa cggcgtcgac
    31741 ctgatcatcg tgggggggcc gacccacgcc ttcgggatga gtcgaccgcg aacgagagag
    31801 gatgcgagcc gacaagcggg cggacatgtc gtgtccgaac ggaacgggct gcgagagtgg
    31861 ctcaccacgg tcgagagaac cggtcggagc cttgcggccg ctgcgttcga cacgcggatc
    31921 gacaggcctc gagttcccgg ttcggctgct cgcggggccg agaagcgtct ccggaagctc
    31981 ggcttccgga tcgcctgtcc gtcagagagc ttctacgtga cgggaacgac cggtccgctc
    32041 gtcgaaggtg aatcggagcg cgcccggcga tggggtgaag ggctcggaaa gggatccctg
    32101 ggcgtcctcg acggagccga ggttgccgag gttcgctccg aggacgtcac atccgggagg
    32161 tgactgaggt gcatacgatg cgacgggtaa ccctcgttct gatcagctcc ctcatggcga
    32221 gcttggccgt gctcgccgca ctcatcgtct ggctcggctg ggtgttaggc gcggcgatcg
    32281 gccttacgat cgctatcgtc gtcgtcacgc tgtacatcac cgtcgtcggc ccatggcaac
    32341 ggcgttgggg aactaccgac gcagaggctc gcggtccgat gcccggcgac gatctgctgc
    32401 gcccaggggc gccctcgacc actcgcgcca tcacgatcga tgccgctcct cgagacgtct
    32461 tcccctggct cgtccagatc ggttacgagc gaggtggctg gtacagctac gactggatcg
    32521 acaacgacgg tcggcgaagc atcgaccgga tcgatccaac gctccaagac ctctccgtgg
    32581 gcgaccgcat ccagatgttc cccggattcg ggcctgtggt cagggagatc gtccccgaac
    32641 accacatcct gagcggtggc gatgccgata cgtggtgtct cctcctggag cccacggccg
    32701 aggggcgaac ccgactcgtg agccgatggc gacaggattg gccgaagacc ttcggcacgt
    32761 tcgcctgggc cctgatctcc gatcccggcg cgttcgtcat ggagcggaag atgcttcgga
    32821 cgatcaggag cctcgccgag gcccggcggg tcgggggtcg gatgggaagg tccacgacca
    32881 gcccgagggg cagcggtccg ccatgatgtt gcggtgaacg gatccacgcc gcacccgctg
    32941 gccggatgcg acgcgcacgc ccggcgagat cgcgaccctg gcggggaccc gcggggtggc
    33001 gaaggccgag gcgccggcga tcacgatgtt cgttctcgcc cccgttgcgg gagcatccat
    33061 cgcgctcggc gccgtcctgt ccccacggtg gcatccgggt ccaccttcgg cctcggaccc
    33121 acgaggtggc tctccgggat cgccttctcc ctcggcgtct tcgtcatcgt cgccggcgcg
    33181 gagcgcttca ccgggaacaa cctgatcgtg atgagagttg ccaccgggca cgtgaagatc
    33241 cgacgcctcc cccagaactg ggacgagccg tgaccggcga ggtgtcgatc gcgctccacg
    33301 ggctcacgaa acgctacggg acccgccgcg gcatcgagga cgtgagcctc acggtggctc
    33361 cgggcgaggt gttcgggttc ctcggaccca acggcgcggg caagaccacg acgatccgaa
    33421 cgatgctcga tctgcttcgg ccgagcgctg gacgagcgac gctcctcggc ctcgactccc
    33481 acgacgacgc cgtgaggatc caccgatcgg tcggctacct ccccggagac ttcgtcgcat
    33541 atcgacggat gaccgggcgc cgctacctgg agttcttcgc ggaggtgcgc gacgtgccgt
    33601 tcggaacggt cgaggccctc agccggaggt tcgcatgtga tctcgaacca cagatcgcct
    33661 cgctgtccca tgggaacgcg cagaagctcg gcgtgatcca agccctgatg catcgtcccc
    33721 gcctcctgat cctcgacgag ccgacccagg gcctcgaccc actgatccag cgtgtgttcc
    33781 acgaactgat gcgggagatc gcggcggagg gtaggaccgt gttcctctcc tcccacgtcc
    33841 tgcccgaggt ggaacggatg tgcgaccggg tggggatcat cggggacgga aggttgctgg
    33901 gcgtcgagga cgtcggcgac ctccgagcca aggcgatccg ggtgctggag ttccatttcg
    33961 cgacgcgggt gccggccacc gtgttcgcgg atctcccagg cgtctccgat ctggaggtcc
    34021 acggggaaac ggtccgctgc tcggtccgcg gctccgtcga cggcgtgctc aaggcggctg
    34081 ccaggtatga agtcgtcgac ctccggagcc acgaaccggg tctcgaggag ctgttcctcg
    34141 agtactaccc cacagccgag gaggcgtcgt gatcgacctc gtccggaagg agctccgcga
    34201 ccagcggcgg gcgctgatcg gatgggggat cggcctctcg gcgatggtgc tgatgtacag
    34261 ctccttctgg cccagcgtgc gtgacaacgc cgatcagttc gagtcctacc ttgagaacat
    34321 ccccgatgcc ctccggagcg tgatcggtga gctcagcctg gggacaccgg tcggctatct
    34381 ccagagcgag ctgttctcgt tcctggggcc ggcattgatc ctcgtgttcg cgatcggggc
    34441 gggggcgcgg agcctggccg gcgaggagga gcaggggacg ttggacctcc tcgtcgtcac
    34501 gccgatcacc cgccggatga tcgtcttgca gaagttcgcc gcgatggtgc tcacggcgct
    34561 cggtctcgga gtgatgctgt ggatctccgt cgtggtggtc ggtcggctct tcggcctcga
    34621 cgtcgcgatc cccgacgtcg cggcggcgat cctacacctc ttgctcctcg gggtcgcgtt
    34681 cggcgcgatc gcgatgttcc tcggagcgtc gcgtggccga cgtgccctcg cgatcggcgt
    34741 cacctcggcc ctcgcggtgg gaacgttcat cctgaacgcg ttcgccccca ccgtcgatgg
    34801 gttccagtgg gccgcgcggc tgagcccctt cttctactac ggggacaccc ttccgcttcg
    34861 caacggcctg cacatcggtc acgcgggggt cctggtcgcc ctcgccgccg tcgccgtgat
    34921 cgcgacggtg gcatcgttcg accgacgtga cctcctgacc tgacctcgac tcgaaggtat
    34981 ggcccgggat cccgccctca cccccgaccg gcccgaagca gccgtcgcgg cgtcatgatc
    35041 ccacgccgct ccaagtcagg cagcaggacg aggaagacct ccgccgtggt cagtgcgtcg
    35101 tcgagcgcgt gatgcgggtg ctcgaccgga acccccagcc gctcggcgac ggcgctgagc
    35161 gagaagtcgt cgttgcgaga ccctgaccgg tccaccagaa gggccagccg gagaacatcg
    35221 acgatgtgcc ttcgccacca cgagggccgt cgcccgaacg ctctcgccag gaacgccgct
    35281 tcgatctccg ccgcccacgc gagtacgtac cggcccgcga gcgcgtcgtg cagatcatcc
    35341 acgaccgctc ccagtggcaa cgcggccgcg agatccaccg gtcggagccc gtgcaccgcg
    35401 atcgacgcgg gcttcaacgg tcggtcgggc cggacctcgc gataggtcga cctccctggc
    35461 aggatcctcc cccgttcgat cgggatcacg ccgaaggaga tgatgtcgtc cccctggagg
    35521 tcgaggccgg tcgtctcgaa gtcgagcgat gcgaaaccgg cctgtcgcca cgatccgcct
    35581 ccgaaacccc gagggcgcgc cgggcggcgg atcatcgggg gcggagcccg tacgtggtcg
    35641 agagcacgcg ctggccggcg atgatcagct tgaaggcctc ccgcaggctc cttcgagtca
    35701 gagggccgag catgcgggga tccacgtggt cgtccgcggg atctcccgcg cgaagctgac
    35761 tcacctgatg ccgcagcctg atccgccaca ggaggcggaa cgcctcttcg agccctacgg
    35821 cgaggtcggg atccagggcg ccgagcgagg cggcgacccg gagtcggtcg cgggtccggt
    35881 tcgaggcgac ccccgatgcg accgcgaacg tccgtgcgag gttcgtgatg ggcgtgatcc
    35941 cgccgtgctt cacatcgagc gttcctgcat gggcgccgtc ccaccccacc aggaactccc
    36001 ggaagaagcc cgtcgggggg cgaagatcga gggccgagga ggcgagcttc cggacgagcc
    36061 atccttcggc cgcggcctgc cggacgagct ggtcgatggt cgcttcgacg tcgagcgccc
    36121 ccgccactcg gcggtagtcc agggcgatgt tcgagaacgc cgtccctccg acgtcggaga
    36181 gacccagctg ttcgcggaac ctccggatcc acgtctcacg atccgctcgc cacgcggcgg
    36241 actcggccat gacgccaccc cgacatcgtg ggatcccggc cccggcgagc ccgtcggtca
    36301 cgcgtcgcgc gagccgttcg aagtacgcgt cagcctcctc cggagaccga tcaggggcca
    36361 cgaagatcag cgcatgatcc tggtccgtca cgagcgactg ctcgtgacga gcctcgctcc
    36421 cgagagcgag ccaggcccat ggtcccggcg gatccccgag ctgcccgatc gccaggtgga
    36481 tcaggcgtcg cgtggtcgcg tcgatcgcgc tggcgatgag gtggccgatg tccacagggt
    36541 cggcctcgat ctccaccaac gggatcacga cggcgggaag ttcggacatc cgagcgaaca
    36601 cctccttcgt cgtctccgca cgatccaatg cgcgcaggaa cgtgaatggg acggtccgct
    36661 cgagcccgat gagatcggtg tcggagacga cacccacgat ccctccatcc gaaccggtga
    36721 ccggcaggtg gtggataccg tgctccagca tcaaccggag cgcgtcgtcc gccatcgcct
    36781 cggcggacac ggtgatcacc ggcgtcgtca tcacccgggc gacgggcacc gagagatcga
    36841 tgccttcggc gacgaccttc gtccggagat cccgatccgt cacgatgccg agaagctcgt
    36901 ccgtgcggac caggatcgaa gagatcgctt cccgcgacat cgtcgccgcc gcgtccctga
    36961 cggaaagggt cgggacgcat ccgatcggct ccctgtgcag gatcgacgag agcgggacgg
    37021 gagagcgagc gatccgctcc gtctccgagg cgagcgccgc ttgccggctc cggcgcgcga
    37081 gcccgcgagc gaggaactcg atgcccgacc gggtgccgat gacctcctcg gcgacctgcc
    37141 gctcgatcag gtagcagatc gtgtcctcga acgccctcac actgagcgtc ggcggaagcc
    37201 cggcaacgag cgagggatgt ccgaacatct cgccctcttc caggaggtcg atcacgacct
    37261 catcgtcgag gagctccacc cctccggtcc gcaccacata gaggaacctc gatggaggtc
    37321 ctccctgctg cagaaccgtc gctccgcggg ggaagaactc gatctcggtg gccgcgcaga
    37381 cccgttcgag cgcctccggc tcgaggacat cgaagggtgg gaagcgaacg aggaacgacc
    37441 ggacctgcat cctgccgaag tatcacccac gccgaccctc ccggcccagg atgcctcggc
    37501 gaccgacccg cggaggacgg gaccttcgac acctccagac cagcgtcggg cgaacagact
    37561 cggatcagac cgacgtcagg ccagggaggc actccgatga ccgcgttgac acgaaccgaa
    37621 cgcccctctg tccgtctacc gctccagctc cctcccgcga ccgtgcgtcg cagagggaag
    37681 cccgggtggg tgggaggacc cgtcgtgctc ctcggcttcg gactcgcgat ctggtgggga
    37741 acggcgacct atctcgagat ggtcgacgac ctcgcgaggc ttcatcgcat ccggatcccg
    37801 agcgcccgcg tggtcgtgct cgacgaggga acccagatcc tctccgttga gagcggtcgg
    37861 ctggccacct tcacggacct ccgattcgcc gtcgtgggtc cggaccgggc cgctgtggcg
    37921 gtggatccct acgagaggga tctccgctac gacgtccccg atgagcctgg ccgagaagga
    37981 cgtgccgttg cgacgttcga agcggactcc ccaggccgat acaccgtccg ggtggccggt
    38041 caggcggcgg cgggcgcgat cctggcgatc ggcgaggatg catcacgtgc cgcgcttccg
    38101 gagatcctgg gcgcgatggc tctcatggcg gcctctctcg tgggcggagc gatcctcctc
    38161 gctctcagga tcgcccgctg gggagatgga cgatgagcgc cccggccgac acccgagctc
    38221 ccgcccgccc gagcgccatc caccgacacg gccggctccg cccgtccgcc ccggggacgt
    38281 ctcgcctcgc cgccgctgcg tgcggagcgg tggtcctgta cggcctcgcc gctctcgtcg
    38341 tgctcccgga gggtgatcca tccctcctcg cgcgcctcat cagcgccgcc gccctcgtca
    38401 ccgccgcctc ggtcgccgtt ggggtcattc gttggggggc tgcacgggcg ggcggggcgg
    38461 tgatcctcct cgtcggcttc acgatgatgg cggtcgccgg gggtgtcgtc gccaaacggc
    38521 tcgtcgatga cgtgtcgttg gtcgacctgc tgggagcggc cgcctccata gcaggcgtca
    38581 tcctcgtggt cgccgggtgg aaactgagcc tagccggcat cgcccgtcgg tggatgcggc
    38641 ccacgatcgc gatcctggcg acactgctgg tcgcgcagtt cctcctccta ccggcaggcg
    38701 tcgcgctcga cgtcacgaac cgtccgcgcc cgacaggatc gggtcggaca cccgcggatg
    38761 tcggcctccg gttccaggac gtgcgcatcc caagcgccga cgggacgctg ctgtcggcct
    38821 ggtggatcgc gcccgagaac ggcgccgcga tcatcctgct cccgggatcg ggatcgacga
    38881 gacataccgt gctcgaccac gccgccctcg tcgcccggca cggatacgga gcgctcctca
    38941 tcgactggcg aggtcacggc gagagcgagg gtcgaagcat ggagttcggc tggggtgccg
    39001 agcccgacgt cagcgccgcc gtctccttcg ccctaggtcg acccggtgtc acacgaggtg
    39061 tcggtgtcct cgggctgtcg ctcggaggtg aggtcggtct cgctgcgacg gcgctcgatc
    39121 cccggatcgg agcggccgtc gccgaaggga tctccgcgcg ctcgtgggcc gacgcgaggt
    39181 tggagccgga tcctcatccg gtcggctacg tcaaccagtg gctgacgttc gtgttcgtgg
    39241 acatcctcgc ccccgagcct cgtccgctcc ccctgatcga tctgatcacc cggatcgatg
    39301 cgccggtact gatgatctcc ggtgctccgc cgagcgagcg gaagctcgat cccgtctacg
    39361 cggacgccgc tccggatgtg atcacgctct ggccgctccc ggacaccccg catacgcggg
    39421 cgctcgggac gcacccggcc gagtacgaac ggcgcgtgat cggcgtgttc gacggggctc
    39481 tcctcgataa cggcgcggtc gctccgaacg gatgaggccc gggcctcgac cgatcgaacg
    39541 gcatcgagag cgaaggcgcg ggctgcaaag gtcccgggag acccgggtca tccggccgaa
    39601 gccgcgcctc acatccggtc ctaccgtcga acggcggtgt ggatcgtgga tcatcagcag
    39661 gggtgagcgg cgcttcgtgg gaagggggag tcatggaaac cagcatcgac gttcctgcgg
    39721 ttccgaagcc gatcggcgtc gggcccgaga tggaagcgct ctggcgcttc ttccgcaact
    39781 gcacatggga aggcacgatc cacgagggcg gtatggggcc gggtacgcca gccatgcggg
    39841 gcgcgggacg tggtgtcgtc gaaccgctcc aggacggacg atggatcgtc ggggacttcg
    39901 aacaggatca gttcctcgag gacgggacgt tcgtcctgac atggcagctt cactgggtgt
    39961 caggctggga cccggaacac ggcgagtacc gagcgtcgat ggtcgacaac tacggacacg
    40021 ccgccgtgta ccgcggatac atcgacggtg atcggctcgt gttcgagtcg atgggcgaca
    40081 cagggccgcg gctccgtttc acgtgggacg cgtcagaccc cgacgtcacc acgtggcgga
    40141 acgagatggc gatcgatgaa ggtccctggt tcctcatcga ggaatacccg atggtgccgg
    40201 cttgacccgg cgcgacccat acggccgtat ggacccgggc tcgagggtca ggggatcggg
    40261 aacgctggcg tcggcgacgg gggtcttcaa catcggttcg gatcgcctgg gcggcgagct
    40321 cgtccatcta ctgccaggaa ctcgctctcg agatgcggga acgccgtccg gcagggctcg
    40381 ttccggtcgc aggtagtgaa gaccgtatcg agcgccgcct ggctcctcgc ggcgatgagc
    40441 tcgaggacag ggacatcgag cagggtccca ccgtcaagca ccgccgcccg cgcgtgctcc
    40501 ccgtgcaggc gcaggtagta cagcgcaagc gtcgccccgt cggtcgccga gcgttcgctc
    40561 ggaccaggaa cggaagcccg cggccaccca tgtcccgtcc acggtcccgg agaagcctga
    40621 cggcttcagg ccgggtgtcc ctcaaggatg gagccggaaa gaccgcagag ccgtgggcgc
    40681 cgtggaccgt tctcgtgatg cggaggagac tcagcccctc cggccccgag atccggcgcc
    40741 accgccgccc agcaggacgc ccgccccgag aacgaagccg aagttgtacc agttgccgtt
    40801 gttgtggatc tcgtacacgt tgacgtcgtc actgaacaac gagatgacga aggtgatcgg
    40861 ggcgatgatc ccgtgccaca gaccgagcca gaatccggcg atctcgccgc tctgagcggc
    40921 accctcctgg acgttcgggc ccggagcaca gctcgccatc gccaacgaga gaacgacggc
    40981 agcagcgacg aatagccatg tccgcttcac gtaccgatac tcacctaggg tccccgatga
    41041 acgcaagccg gccgcctccc tccacgatcg gagcccgaag gtcccagccc caccggtcct
    41101 gtcggccctg agcgaggccg aaagcggctg gaaccatgga cccgaaaggg ggcgatcccg
    41161 atgacgcctc ttccgacgta cgacagacga caccctgtat cggttgcccc ggttgcgccc
    41221 gcgttgccca gaccctcgat cgttcggaac agcaaacctc cgatcgccct gaccgccgcg
    41281 ctgttgctgg tcgcggccct catcggagca ggctccatct tcgcatggga caggaccggc
    41341 tcccgggatc ggtcgggcac gctcgcagac gcgatcgcgc agcgagatgc agcgatgagc
    41401 gagatcgcga ccctgaacga ccgtctgaac gatcttcgtt ccgggttgac ggaagcgaga
    41461 ggtgcggtgg cagctttgga gggatccctc tcggctgccg aggcggagct gggagccatg
    41521 ctgggaccga ctctgccgga tgggaagcac ttcggtgccc tcatcgccct cggcgccgca
    41581 cagcaacctc cgcggctcgt catcgatatc cagcagtggt tcaccgatga ggaggcgatc
    41641 gccgccgcga tcgaggacgg cgtgatcgaa cccggagaga tcatcccgaa cggctactac
    41701 atccggaacg acaacccgcg ctggcggacg atcgagatcg acccggcggc gaaggtgtcg
    41761 ctcacgacgt acccgtatgg ccagatcgat gatcctcaga tcgtgagcct cgagcgattc
    41821 gcggatctgg cgatcgattg gtccgagtac tggatcacca tggcggaagg gaaggttgta
    41881 gcgatcgggc agcagtacat cccttaacac gcagaacgcg aacggaggag atcgagacga
    41941 tcgaagagga gctgatccca tgaccaagga ggtcgtggag ccggcaagtc atcggtggga
    42001 aggagagcgt catgcagtgg gatctcggac tccgagggct ggggttgctc gtcctcatat
    42061 cgcttggctt cggcgtcgtc gcccagctca tcggcgggaa ggccaccaca cactggctct
    42121 ggctcatcgc cggggccgcc tacttcctcg caggcctgtt catcagtgag gtctggttcg
    42181 gctgggcgac ggagaaggaa ctgcaaccga acatcgacgg tctgtcgttc gacgaggtcc
    42241 tcatcggcct ggtcccgggg atcctcgcgg ttctcgtcac caggaaggtg agtcgaagcc
    42301 ggtcgcatcc gtcacatcgg tcgaggatcg tgtactgaga acccgtcgac gaccacgaag
    42361 tggtcaagcg accggatgac tgttcatccc ggcggtgcct ctcgtcctga tccgggccga
    42421 cctgcacggt tcttcgcccc agggtcatcg acctggcgaa ggtcacggaa cgacagggtc
    42481 gttcgaccgt gggcctcaac cgaaggagaa ccatgctggg tgtggaggtg gatagtgatg
    42541 acaggtatcg ggatcccacc catcgatccg ggcataaccg gcggcggcgg gagcatctgg
    42601 ccggagatcg tcgcagcgat cgtgcttctc gtggtcttcg ctgcggccat ctggacatac
    42661 atccggaacc gccccgccca cacggatcgg accgtgagcc agaccgagct ccccaaggca
    42721 gcctgatcgt caccgcagcc ccgggagtcc ctcccgaggc tccttggcac cctcgccccg
    42781 ggggtcgctg gtcaggtcga ttccgcccat caggatcagg aggaagagga tgagcgagca
    42841 acggcagcag cgggagtgga ccgaggaact gaaggtgcag ggcgaagcgc tgctcggcac
    42901 ggtgaaggag ctcgcgcatg agggcaacgt ccgcaggatc atcatcaagg acgctcatgg
    42961 gcagacgctg atcgagatcc cgctgaccgt gggcgtggtc ggagccctgg tggcgccggt
    43021 cgcagccgcc gtgggtgccg tcgcagccat ggcaaccgac tgctccatcg tcgtcgagcg
    43081 acgtgaggac ggtcccccat cgacgtgagc gcggcggacc cctgatcctc ggccctacct
    43141 gacgggaagc acttcggagc cctcatcgct gtcggggcca accaggagcc gccgcggctg
    43201 gtgatcgacg tcgaacagtg gttcacggac caagcggcgt tcgacgccgc cgaagaggac
    43261 ggggtcttcc tcgatcaccc gacgtacatc cggaacgaga acccgcgctg gcggacgata
    43321 gatcgacccg gctgcgacgg tgtccaagat cccgctcggg tttcgctcgt actcgaccga
    43381 cgtcaacctg agcgagttcg gggagctctt ctcctcctac gagggtcgag accttcgggg
    43441 gtcgccgtac tggatcacga ctgacgacgg gaaggtcgtg gcgatcgagg agcaactcat
    43501 ctcgtagcca cctcgtcccg cggtcctgag ggccaggacg ctggtcccgg cctcaccgtg
    43561 acgtccgtga ccgtgctcgg tcgaccacga ggatcccgat gggagcaacg agtgccgcac
    43621 tcaagcagag gagccacccc gacgcggtga gggacgtggt gtcgaagacg ccgtggaggg
    43681 aaggaacggc caccaccgcc agctgaagga tgatgccgcc gcccacggcg atcagcaacc
    43741 cccgggcggc gaggatcgaa cggatcggcg cgggcgttcc agccggacga tcgaggtgga
    43801 cggcgaacgc atagagcaga tgcgacagga cgaggatggt gaacatcgcc gtccgtgccg
    43861 actcgaacga cttgtcgaag gtgaaccgca cgatcacgaa ggtgcctacg gacatgagcg
    43921 cgatcgacat cccccgcgcc ccgagcatcc gcgctctccg ccacgacagc aggctcgcgg
    43981 tgtcgacccg atccgtctgt gcagaggaac ccggctcgac cccgagggcc accgccggaa
    44041 gaccatcggt gaggaggttg atccagagga tctgtagggg cgtgagcgtg gcaccggcgt
    44101 cggggaacaa gagcagcacc gtcacgacga ctgcgacctc ggacagattg ccggcgacga
    44161 ggtagtcgat gacatttcgg atgttcctga cgagccggcg cccctcttcg accgcatgca
    44221 cgatcgtgcc gaggtcgtcg tcggtgatga ccatgtcggc ggcttcccgg gcgacgtcgc
    44281 tgcccgtcct gcccatcgcc acaccgatgt ccgcctgccg cagcgcagga gcgtcgttca
    44341 cgccgtcacc ggtcaccgcg acgacctccc cccggtcctg gaacctgcga acgatggcca
    44401 gtttgtcgtc cggcttgacg cgagcgaaga cgctgacgcc cgccggatcc gcctcgccga
    44461 gctgatggcc ttcgaggacg tgaccgtcgg gcgggaggag gccgacctca cgcgagaccg
    44521 aggtcgccgt accggcgtga tctcctgtga ccatcacgat ccggatgccg gcggaggccg
    44581 cgtcggcgac cgccgatgcg gcctccgccc ggacggggtc gcggagaccc acgatgccga
    44641 ggaacgtgaa cccctcctcc ggcggccgat ccggctcgat gtcaggtcct tctctgatgg
    44701 cgaaggcgat cacacggctt cccgacgatg cgaggtcctc cgcgaa
//
LOCUS       DASUYD010000145         3339 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1181616, whole genome shotgun sequence.
ACCESSION   DASUYD010000145 DASUYD010000000
VERSION     DASUYD010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181616"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF195_07965"
     CDS             complement(<1..481)
                     /locus_tag="VF195_07965"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase family protein"
                     /protein_id="HEX6580792.1"
                     /translation="MTSVTGLERFLEAADYEARRRGGSSVSRAHRPVITVSRKAGSGA
                     HVVAQELVGRLQARDPKASPPWTVFDRDLVAEVLREHHLPDRFAGSMPEDRVSEIADT
                     LDGLFGLRPSSWTLVRKTAETILHLAEIGNVIVIGRGANILTDRFADALHVRLVGSVR
                     "
     gene            1074..2549
                     /locus_tag="VF195_07970"
     CDS             1074..2549
                     /locus_tag="VF195_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006034965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6580793.1"
                     /translation="METAPFTPTDSAHAPLKRELGLPTATALVVGNMIGSGIFLLPAS
                     LAAVALVSGSGSLLAWALTGLGAMLLAAVFATLGRAYPKTGGPYVFAHRAFGNFIGFQ
                     TAWGYWIAAWAGNAAIAIAFTGYLTVFWTSLANSKIAMAGVTIALVWLLTLVNILGVR
                     ETGIVQLVTTVLKFVPLLLIGIVGLFYMQADNFTPFAPNGLGTGNGMWGGITAAAALT
                     LWAFIGLESATVPAEEVRSPEKTIPRATVLGTLVTTLVYVVATVAIIGIVPLGQLADS
                     SSPFAAAASTMFGGAWGKVVAAVALISVFGCLNGWILIQGRVPLAAANDGLFPERFAR
                     VHGQRGTPVFGLVVSSLLISALILMNYSKGLVDQFTFIILLATLTTVVPYAFAAAAEV
                     YLFITERERFVSRSVIRPTIVATLGFAYAIFAIWGIGWDIIGKGFLLLMVGIPVFVYM
                     AWRHRPAPKALATPEPTAISVSDMPPLVVSEPRRPLPSEGR"
     gene            2554..>3339
                     /locus_tag="VF195_07975"
     CDS             2554..>3339
                     /locus_tag="VF195_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6580794.1"
                     /translation="MTRRVLIMGAAGRDFHDFNVVYRDDPGSDVVAFTATQIPFIADR
                     RYPAELAGSRYPDGIAIHDESELPDLIRKLEVDDVVFAYSDVSHEYVMHKASEVLAAG
                     ANFVLLGPDATMLKATVPVVAVCAVRTGAGKSQTTRFIAELLKREGLKVVAVRHPMPY
                     GDLVAERVQRFETLEDLERAKVTIEEREEYESHIRAGTIVYAGVDYGQILERAERECD
                     GLLWDGGNNDLPFYRPDLHITVADPLRVGHERAYHPGEANVRMA"
BASE COUNT          526 a         1122 c         1116 g          575 t
ORIGIN      
        1 gccgcaccga gccaacgagc cggacgtgga gcgcgtcggc gaaccggtcg gtgaggatgt
       61 tggcgccgcg gccgatcacg atcacgttgc cgatctcggc caggtggagg atcgtctccg
      121 ccgtcttccg gacgagggtc cagctggacg gacgcagtcc gaacagcccg tcgagggtat
      181 cggcgatctc ggagacccga tcttcgggca tggatccggc gaaccggtcg ggaagatggt
      241 gttcccggag aacctcggcc acgaggtccc gatcgaacac ggtccacgga ggtgatgctt
      301 tcggatctcg agcctggagc ctgccgacga gttcctgcgc cacgacgtgc gcccccgacc
      361 ccgccttgcg cgagacggtg atcaccggcc ggtgggcgcg cgacaccgac gacccaccgc
      421 gccgccttgc ctcgtaatcc gcagcctcga ggaaccgttc caaaccggtc accgacgtca
      481 tcggcccacc tcctgtggcc tccccgccaa catacgcccg tccttgtcgg gctcttccgc
      541 cgaacgtcac ggtgtcccag gtacgaccgg ccctgtcgag cgggcgcgga gggacgggcg
      601 ggggcacccc cagggcttcg ggcagcctgc ccgtgacgct gcaacgcgga cacgcggaca
      661 cgcggacacg cggacgcgca gacacgcgga cacgcggcga ccgggtcgga ccggtggagc
      721 cagaaccgtc ggccctcatc agaggtggag tgatgcgagg ttcgcgggtc cggacgagcg
      781 tcgaccggcg catcttctgg attgcgcgcc agtcgactgg gtcattccac cgatcggtcg
      841 gtggacagca gggaccatcg atccgtctgg cgtcggcatc gctcacgtcg gttggtcggg
      901 cttcaagcaa tctcagtgtg atcctctcgc gggatcacat cggccatcgg atcgcgacgg
      961 tgccaagggt cccatgggaa gcatgacctt cggccggggc gtcgatgccc acccccgata
     1021 gcgtccgaga cgggcggagg gcatttcgcc ctcccatcca gaaggagggt gccatggaaa
     1081 cggcgccgtt cactcccacc gactcggctc acgcgccgtt gaagcgtgag ctcggcctac
     1141 cgactgccac cgcgttggtc gtgggcaaca tgatcggatc gggaatcttc ctgctccctg
     1201 cgtccctcgc ggcggttgcg ctcgtctcgg gctccggctc attgctcgcc tgggcgctga
     1261 ccggtctcgg agcgatgctc ctggccgcgg tcttcgcgac gctggggcgt gcatacccga
     1321 agaccggagg tccctacgtg ttcgcacatc gggctttcgg caacttcatc gggttccaga
     1381 ccgcgtgggg atactggatc gccgcatggg ccggcaacgc cgcgatcgcg atcgcgttca
     1441 ccggctacct cacggtgttc tggacatcgc tcgcaaacag caagatcgcg atggcgggcg
     1501 tcacgatcgc cctcgtctgg ctgctcaccc tagtgaacat cctcggggtg cgcgagacgg
     1561 ggatcgtgca gctcgtgacg accgtgttga agttcgtgcc actcctcctg atcggcatcg
     1621 tcggcctgtt ctacatgcaa gccgataact tcacgccgtt cgcgccgaac ggtctcggga
     1681 ccgggaacgg gatgtggggc ggcatcaccg ctgccgcggc gctcaccttg tgggcattca
     1741 tcggcctgga gtccgccacg gttcccgcgg aggaggtcag gagccccgag aagacgatcc
     1801 ctcgagccac ggtgctcggc acgctcgtga ccacgctcgt ctacgtcgtc gctacggtcg
     1861 ccatcatcgg catcgtcccg ctgggtcagc tggcggactc gtcgagcccg tttgccgcag
     1921 cagcgagcac gatgttcggc ggagcatggg gtaaggtcgt cgccgccgtg gctctgatct
     1981 cggtgttcgg atgcctgaac ggctggatcc tgatccaggg ccgcgtcccg ctcgccgccg
     2041 cgaatgacgg cctgttcccc gagcgattcg cccgcgtcca cggtcagcgc ggtacgccgg
     2101 tgttcggcct ggtcgtctcc tcgttgctga tctcggcgct catcctgatg aactacagca
     2161 agggtctcgt cgaccagttc acgttcatca tcctgctggc cacgctcacg acggtcgtgc
     2221 cgtacgcgtt cgctgcggcg gccgaggtct acctgttcat caccgagcgt gaacgcttcg
     2281 tttcccggag tgtgatccgg cccacgatcg tggcgacgct cggcttcgcc tacgccatct
     2341 tcgcgatctg ggggatcggc tgggacatca tcggcaaggg gttcctcctg ctgatggtcg
     2401 gtatccccgt gttcgtctac atggcctggc gtcatcggcc ggcgccgaag gcgctcgcga
     2461 cacctgaacc gacagcgatc agcgtctcgg acatgccgcc gctcgtcgtc tcggagccac
     2521 gccgcccgct cccgtcggaa ggaaggtgag gacatgacac gccgcgtctt gatcatggga
     2581 gccgccgggc gcgacttcca cgacttcaac gttgtctacc gcgatgaccc gggatctgac
     2641 gtggtcgcgt tcacggccac gcagatcccg ttcatcgccg atcgacggta tcccgccgag
     2701 ctggcaggtt cgcggtaccc ggatgggatc gcgatccacg acgagagcga gctcccggat
     2761 ctcatccgca agctcgaggt cgatgacgtc gtgttcgcgt attcggacgt cagccacgag
     2821 tacgtgatgc acaaggcgag cgaggtgctc gccgccggcg cgaacttcgt gctcctgggt
     2881 cccgacgcca ccatgttgaa ggccacggta ccggtggtgg ctgtgtgcgc ggttcgcacc
     2941 ggagcgggga agagccagac cacgaggttc atcgccgagc tgctgaagcg ggagggcctc
     3001 aaggtcgtcg cggtcaggca cccgatgccg tacggcgacc tcgtggccga gcgggtgcag
     3061 cggttcgaga ccctcgagga tctcgagcgc gcgaaggtga cgatcgagga gcgagaggaa
     3121 tacgagtcgc acatccgggc cggcacgatc gtctacgccg gcgtcgacta tggccagatc
     3181 ctcgagcggg cggagcgcga gtgcgacggc ctgctgtggg acggagggaa caacgacctt
     3241 ccgttctacc ggccggacct gcacatcacc gtcgccgacc cactccgggt gggccacgag
     3301 cgcgcgtacc acccaggcga agccaacgtt cggatggcc
//
LOCUS       DASUYD010000146         4161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1182716, whole genome shotgun sequence.
ACCESSION   DASUYD010000146 DASUYD010000000
VERSION     DASUYD010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4161
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182716"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            601..786
                     /locus_tag="VF195_07980"
     CDS             601..786
                     /locus_tag="VF195_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580795.1"
                     /translation="MIGMGVPPVEPIAGAESSIWPAIITAIVFGTLLVATVWTYLRNR
                     PAATLPHEEEHQLLKAA"
     gene            1094..1591
                     /locus_tag="VF195_07985"
     CDS             1094..1591
                     /locus_tag="VF195_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580796.1"
                     /translation="MQTETKPEPIVRCFEPTARFSRVTRIANVFVRPLLRASAGKRIH
                     ELALLCFEGRRSSNHYEVPVEYHELDGEPLILTASAWKANLRGGADVEFVHDGVRAPM
                     RAELVEDPDEVATVYERLIERIGVEKAKSTKIGLEVIGDGMPTHEQIRGAIASRRTVV
                     RLQPR"
     gene            1871..2110
                     /locus_tag="VF195_07990"
     CDS             1871..2110
                     /locus_tag="VF195_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580797.1"
                     /translation="MISAASTTPQNTDCTVGPDLVAQSHEQQIADDDLSGRNILRAPR
                     LGSNGLTGDHLVAPELGDRCRHLVARVFRVTCVLG"
     gene            complement(2440..2613)
                     /locus_tag="VF195_07995"
     CDS             complement(2440..2613)
                     /locus_tag="VF195_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580798.1"
                     /translation="MSDAVDAAQKQPADPADAGEDECEGPTTRLGEEGGSQRERPELG
                     EHGERHRYVADRP"
     gene            2748..2903
                     /locus_tag="VF195_08000"
     CDS             2748..2903
                     /locus_tag="VF195_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580799.1"
                     /translation="MPAVVLVVLLAAVWWKALRSREEDLVGVPSLVALCAVLACSPVF
                     SSSTRDG"
     gene            2900..3157
                     /locus_tag="VF195_08005"
     CDS             2900..3157
                     /locus_tag="VF195_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580800.1"
                     /translation="MTPWAAIAGRDPRRVPIASAWAIVVLTAGVSIALFQYADSLKGL
                     GAAMLLARNGLCLFLPVWFLASGRPAVAAVSIRERLRPDLA"
     gene            3481..3741
                     /locus_tag="VF195_08010"
     CDS             3481..3741
                     /locus_tag="VF195_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580801.1"
                     /translation="MAERVPDELAPCAREEEERLRLPEELNVERSALAPGLLVAHVRR
                     ELRHRRPVDVVDGYPKRLERQDIGRRATDDFERIRVERINHQ"
     gene            complement(3898..>4161)
                     /locus_tag="VF195_08015"
     CDS             complement(3898..>4161)
                     /locus_tag="VF195_08015"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580802.1"
                     /translation="LPPTHIGVQAGPVVFRDGDVYGRTVNVASRIADQARAGEILTSH
                     ETVERVNGGEVSFEQARSVELQGVERPVTLYRVVGRHDHRMRR"
BASE COUNT          639 a         1388 c         1446 g          688 t
ORIGIN      
        1 gggcggttcg gcccggcgtc ggggggccga tctggccggc ccgggctggc cgcgctcggc
       61 ggatccgcga agttggtacc ggcccgggcc cagatcccgt ccggtgaccg ggtcccgagg
      121 atatcccgag tcaggcggag ccacgagtgg ctgctgccgg cctgcatacg tctctcggtg
      181 aaggaagctg agagcttgcc atcgaccgat gccgatgatc gatggttgag ttgtcctcgg
      241 tggccgatag agtccgagcg agtgggtgac gcgcgccgtc ggcccagatg ctagtcagcg
      301 atcgcgctcg cggatgcaga cgccctcgcg gaggaggcct ttcggccgat attccgccgc
      361 tcaaggatgc ctacggtacg agcatgggcc ggagtgtccc agaggtgacg tcgagctcga
      421 cgggcttcgc ccctttcctg cggttccagc cgggggcacg gggagatgtc gtgttccggg
      481 gttcgagatg gatgaaacca cgttctcgct ggtgggacca agggcacaca tcagtagggc
      541 ccttcggcca tagatcgtcc tcgtgtcggg atacatgctg gggatggagg tgagcgagac
      601 atgatcggca tgggagtccc gcctgtcgaa cccatcgcgg gggcggagtc cagcatctgg
      661 ccggcgatca ttaccgctat cgtgttcgga accttgctcg tggcgacggt ctggacgtac
      721 cttcggaacc gtcccgccgc gacgctcccg catgaggagg agcaccagct cctcaaggcc
      781 gcttgagcga cgtcgagaag gcgtgagtca cgagggggcg gcccccgggg acgactcggg
      841 gccgccccgc cctggtgcgc gatagattcc taccgcttcc gggtgggagc atctgatgct
      901 cctcttcggc cgcgctctcg gtcgcgaact cgtcattcct cagcccgtcg cagatgtcga
      961 cgtcactcgt gcgggagacg gcccgccggt cacggggcta acggcctaag ggaccggctc
     1021 cgcggcgcgg ttgactggtg tcgggacggc gggatggcgg atggaccgcc ctccgccggg
     1081 aggtgaagat ccgatgcaga cggagaccaa gcctgagccc atcgttcggt gcttcgagcc
     1141 gactgcccga ttcagcagag tcacgaggat cgccaacgtg ttcgttcggc ccctcctgcg
     1201 ggcctcagcg ggcaagcgga tccacgagct ggcgctgctg tgcttcgaag gtcggcgcag
     1261 cagcaaccac tacgaggtgc cggtcgagta ccacgagctg gacggcgaac ccctgatcct
     1321 gacggcgagc gcctggaagg cgaatctccg aggcggggcg gacgtcgagt tcgtgcacga
     1381 cggggtccgc gccccgatgc gcgccgagtt ggtcgaggat ccggacgagg tggccacggt
     1441 ctacgagcgg ctgatcgagc ggatcggagt ggagaaggcg aagtcgacca agatcggcct
     1501 cgaggtgatc ggcgacggga tgccgacgca cgagcagatc cgcggcgcaa tcgcaagtcg
     1561 acgcacagtg gtccggctcc aaccgcggta ggcgtcaggc gggagctgcc actttgacag
     1621 ctcccgtgct gggcgacgaa cgcaccaaga gcgccaacag ctcagccagg accgccgtca
     1681 caagactcag cgcgggacat tcccgtgcga caagcgaccc aacgtggaga tcaccgaagg
     1741 gaaggtccgg ctccctgacg ggccgttccc gtcggcgcaa cccagacggt cgctcggcct
     1801 cgcacaacgg cggcaagact ccgactgacc atcgtgacgc tgttcgcact gtcgggatcg
     1861 ccagagcgtt gtgatcagcg cggcgagcac gacgccccag aacaccgact gcaccgtcgg
     1921 tcccgatctc gtcgcccagt cgcacgaaca gcagatcgcc gatgacgatc tcagcggtcg
     1981 gaacatcctt cgggctcccc gtctcggatc gaacggcctc accggcgatc atctcgtggc
     2041 ccctgagctc ggcgaccgct gccgccatct cgtggcccgc gtgttccgcg tgacctgtgt
     2101 gctcgggtga ggcagcacgc gcttcgccaa cggcatgcat cgcgtccctg tcactctgga
     2161 gacggatcgg gttccatcag cgggtcgcag atgtgctcgg ggaccgaccg gccggcgcaa
     2221 tgggaaaccg cagtcctgga cccagcggcg gagctcggcg gcaacgcggt cctcgacacg
     2281 gcggggcagc tcgagcctgc aaccctcagc ggtcgaagtc gcacggcgag cgaacgggcg
     2341 gctgccagcc ccatccaaga gctcgtcgtc gggatcggtc ggtctggctt cagccgggcg
     2401 tgcgaacccg gcataaacgc cttcgctcga cagcggaggt caaggtcgat ccgcgacgta
     2461 ccgatgccgc tcgccgtgtt cgccaagctc tggccgctcg cgctggctcc cgccttcttc
     2521 gccgaggcgc gtcgtcggtc cctcgcattc gtcctcgccg gcgtcggcgg gatcggctgg
     2581 ctgcttctgg gcggcatcga cggcgtccga caagtgctgt ccttccggtc ggccaccggc
     2641 tgggaggtcg agagcaccgt cggcgcgctc gtctgggtgt tcggcggggg cgaacccaca
     2701 tgggaacggg ggctgtccgt gtcggttcgt cgcagggcct cacgtgggtg ccggcggtgg
     2761 tgctcgtggt cctcctcgcc gccgtctggt ggaaggcact caggtcgcgc gaggaggacc
     2821 tcgtcggcgt cccttcgctc gtcgcgctct gcgcggtgct ggcgtgttcc cccgtcttct
     2881 cctccagtac gcgggatggt tgacgccgtg ggcggcgatc gcgggtcgtg acccgcgccg
     2941 cgttccgatc gcctccgcgt gggcgatcgt cgtgctcacc gcgggagtga gcatcgcgct
     3001 cttccagtac gccgacagcc tcaagggact cggggcggcg atgctgctcg ccaggaacgg
     3061 attgtgtctc ttccttcccg tctggttcct cgccagtggg cggcccgcgg tggctgcggt
     3121 gagcattcgt gagcggcttc ggcccgacct tgcgtgaagc ttctgtcttc tcatctcatc
     3181 taggcaggaa ctggatccct cacgaggtgc ggtcgcgacc ggcgaagcgc gacaggtaga
     3241 gccacgtcgg tcggaacccc cgccgttcgt acaggcggac cgcgtcgacg ttgcctggga
     3301 gaactcgatc acctgatcgt cgacgccgag cagcgcgaac tcgtcgtcga cggcgtcgag
     3361 cagcgccgag ccgatcccgg ctccgcggta ctcgggtagt accgagatcg attcgagctc
     3421 cgcgactcgg tcgccggtgc gccacgtatc gctccaccat gactcggccg agccgaggat
     3481 gtggccgagc gcgtacccga cgagctcgcc ccgtgcgcgc gcgaggaaga ggaacgtctc
     3541 cggcttcccg aagagctcaa cgtagagcgc tcggcgctcg cgccaggtct cctcgtcgct
     3601 cacgtacggc gcgagctccg gcatcgacgc cccgtggacg tggtggacgg ctacccaaag
     3661 cggctcgagc gacaggatat cggccgccga gccacggacg atttcgagcg gatccgagtc
     3721 gaaaggatca accatcaatg agcggggtcg cgcgcgtcag cccaatcgtg ggaactggcc
     3781 atggcgtcct ctcctcgccg gctggtcatc ggtcgatgcc ctttcctcca tggatctcgc
     3841 cggtcggtct tgctgagggc gggtgcagat cgctggagcg atcgacatgc gtgcgcctca
     3901 acggcgcatc cggtgatcat gccgccccac cacccggtac agggtcacgg gcctctcgac
     3961 gccctgcagc tcaacggagc gcgcctgctc gaacgacacc tcaccgccat tcacgcgctc
     4021 gacggtctcg tggctcgtga ggatctctcc ggccctggcc tgatcggcga tccgcgacgc
     4081 cacgttgacc gtgcggccgt agacgtcccc gtcgcggaag accaccgggc ccgcctggac
     4141 gccgatgtgc gtgggaggca g
//
LOCUS       DASUYD010000147         2259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_962577, whole genome shotgun sequence.
ACCESSION   DASUYD010000147 DASUYD010000000
VERSION     DASUYD010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962577"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF195_08020"
     CDS             complement(<1..379)
                     /locus_tag="VF195_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580803.1"
                     /translation="MLGTVVIVLVVAALGGLLVFRAIRGDLPSEAEKAEQIGRLKATV
                     LPVLEDLKVEYFMDAAGCAILTYRRGDFFDGDPEECRGSTGRAVQFDDVARADQQLIK
                     AALAASGTPIERVGGTFLSDGRVR"
     gene            378..578
                     /locus_tag="VF195_08025"
     CDS             378..578
                     /locus_tag="VF195_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580804.1"
                     /translation="MVLGSLKNLGFTGPEPMGASDRPRTLYADTGTLNELVGVAVRTS
                     LAFARVSGGMGASPVTRREGYI"
     gene            complement(639..1064)
                     /locus_tag="VF195_08030"
     CDS             complement(639..1064)
                     /locus_tag="VF195_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEX6580805.1"
                     /translation="MKTTTVETAERGRSPLGAHIRFLMDGPHGNMIHSTVPPGMVGRA
                     CHFRTIDEYWYVLSGEGEIWRRAPDGQESITRLTPGVCVDIPLGTAFQYRCTASVALV
                     CTVTALPPWPGDEEAVIVDGPWVPRVDSEPGGQRSDWPT"
     gene            complement(1246..>2259)
                     /locus_tag="VF195_08035"
     CDS             complement(1246..>2259)
                     /locus_tag="VF195_08035"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6580806.1"
                     /translation="ALITPWNFPLVIATWKLSPALACGNTIIVKPASLTPLSLLRLAD
                     ILAEAGLPLGTMSVLPGPGSSVGNALVTDPRVAKVGFTGSTEIGSAIMALAARNITRI
                     SLELGGKSANVVFADADMDTCVERSMWAVFGNTGQDCCARSRIFVERPAYDGFVEALS
                     ERTEGLRVGQPLSEETDLGPLISSGQRQTAVDYLEIGQKEGARLVTGGDVPDVPDGAN
                     GFYLRPAILADVDNAWRVAQEEIFGPVACVIPFDTEEEAIRLANETEYGLSGSIWTRD
                     LGRAIRVAKGIRTGVLSVNSSHSVHTEAPFGGYKRSGIGREMGMHAVNLYTEVKNVYF
                     SEG"
BASE COUNT          382 a          771 c          756 g          350 t
ORIGIN      
        1 acctgacgcg gccatcactc aagaacgtgc caccgacccg ctcgatgggg gtcccggacg
       61 ccgccagggc ggccttgatc agctgctggt cggcgcgcgc aacgtcgtcg aactggaccg
      121 cgcggccagt ggagccacgg cattcctcgg ggtctccgtc gaagaaatcg ccgcgcctgt
      181 aggtgaggat ggcgcacccg gccgcgtcca tgaagtactc gaccttcagg tcctcgagga
      241 cgggcaggac cgtggccttg aggcgcccga tctgttcggc cttctcggcc tccgacggca
      301 gatcgccgcg gatcgctcga aaaacgagga ggcccccgag agcggccaca acgaggacga
      361 tgacgacggt gccgagcatg gtcctgggca gcctcaagaa cctgggcttc accggccctg
      421 aacccatggg agcctccgat cgaccgagaa cactctacgc cgacaccggc acgctgaacg
      481 agctcgtggg cgtcgccgtg cgcacgagcc tggccttcgc ccgagtcagc gggggaatgg
      541 gcgcgtcacc agtcacgcga cgggaagggt acatctgact gacccctcgg ctgcgcgagc
      601 attcaggagt ttcctcagag aagtcggagg gcggaaggtc aggtgggcca atccgagcgc
      661 tggcccccgg gctccgagtc cacacggggt acccaaggcc cgtccacgat cacggcctcc
      721 tcgtcgcctg gccacggcgg cagggccgtg accgtgcaga caagggccac gctcgcggtg
      781 cagcgatact gaaaggctgt gccgagcggg atgtcgacac acacgcccgg ggtgagccgg
      841 gtgatgctct cttggccgtc cggcgcgcgc cgccagattt ctccctcacc cgagaggacg
      901 taccagtact cgtcgatggt ccggaagtgg caggcgcggc caaccatccc cggcggcacc
      961 gtcgagtgga tcatgttgcc gtgcgggcca tccatcagga aacggatatg agctcccagg
     1021 ggcgagcggc cgcgctcggc ggtttcgacg gtggtggtct tcactgtctg taactcccgt
     1081 caagggacag gggtaaggct agccggtcct ccactgctgc gtgtaactgc agacgcggcc
     1141 tttcgtgctc cccgcgggcg aaagcaccat ctggtgcacg agcgtgctgt cccccggggt
     1201 tcttcggctt ccaccagtcc aagcggctga acgagcgagg gtgcgttacc cctccgagaa
     1261 gtagacgttc ttcacctcgg tatacaggtt gaccgcgtgc atccccatct cgcggccgat
     1321 cccgctccgc ttgtagccgc cgaacggcgc ttcggtgtgg acgctgtgcg acgagttgac
     1381 gctcagcaca ccggtgcgga tacctttggc gacacgaatc gcccggccga gatcccgcgt
     1441 ccagatcgaa ccggagaggc cgtactcggt ctcgttcgcg aggcggatcg cctcctcctc
     1501 ggtgtcgaac gggatcacgc acgcaacggg cccgaagatc tcctcctgcg ccacccgcca
     1561 cgcgttgtcg acgtcggcga ggatcgccgg tcgcaggtag aagccgttcg cgccgtcagg
     1621 cacgtcgggg acgtcaccgc cggtcaccag ccgcgcgccc tccttctgac cgatctccag
     1681 gtagtcgacc gcggtctggc gctgcccgga cgagatcagc ggtccgaggt cggtctcctc
     1741 cgagagcggt tgccccacgc gcagcccctc ggtccgttcg gagagcgcct cgacgaaccc
     1801 gtcgtaggcg gggcgctcga cgaagatccg gctccgcgca cagcagtcct ggccggtgtt
     1861 cccgaacacc gcccacatcg agcgctccac gcatgtgtcc atgtcggcgt cggcgaacac
     1921 gacgttcgcg gacttgccgc cgagctcgag cgagatccgg gtgatgttcc gagccgcgag
     1981 cgccataatc gccgatccga tctcggtcga ccccgtgaac ccgaccttgg cgacccgagg
     2041 atcggtgacg agcgcgttgc ccaccgagga gccggggccg ggcagaacgg acattgttcc
     2101 gagaggcaac cccgcctccg ccaggatgtc cgcgaggcgt agcaacgaga gcggcgtgag
     2161 cgaagcgggt ttcacgatga tcgtgttccc gcacgcgagg gcgggcgaga gcttccacgt
     2221 cgcgatcacg agcgggaagt tccacggcgt gatgagcgc
//
LOCUS       DASUYD010000148        55928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_79507, whole genome shotgun sequence.
ACCESSION   DASUYD010000148 DASUYD010000000
VERSION     DASUYD010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 55928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55928
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79507"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..477)
                     /gene="bcp"
                     /locus_tag="VF195_08040"
     CDS             complement(13..477)
                     /gene="bcp"
                     /locus_tag="VF195_08040"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEX6580807.1"
                     /translation="MTALKVGDPAPGFSLLEQHGETVRLEDYRGKRLLVYFYPEADTP
                     GCTVQSCDLRDHRQELAGVGLDVVGISPDDPEKQLRFDEKYHLGFPLLADEDHDVATA
                     WGAWVEKHRGGHTYMGILRSSFLVDEEGRIQQVWSPVTPEDTVPNAIAAIAA"
     gene            complement(512..1147)
                     /locus_tag="VF195_08045"
     CDS             complement(512..1147)
                     /locus_tag="VF195_08045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX6580808.1"
                     /translation="MALRVESTSVTDGQPIAEIHAVATPTADGKAEMAGRDLSPHLRW
                     SGEPEGTRSFAISVVDPDVPADRTRMGVEGLSLGDDEPRVDFAHWLVADIPPSVHELA
                     EGAGGDGFVAHGKPSEPPALGAVTGQNGYRGLFEGNDDLEGTYGGWDGPFPPWNDEHV
                     HHYETTVYAIDVDSLGLGSGFMLEEFRAAIDGHVLDQGTIVPVYSLNPDLR"
     gene            complement(1230..2288)
                     /locus_tag="VF195_08050"
     CDS             complement(1230..2288)
                     /locus_tag="VF195_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HEX6580809.1"
                     /translation="MSGDPEPTRTLTTRELNRALLARQHLLERATTSVPKTIERLGGL
                     QTQHAPAGYIALRTRHEGFRRDELTRALHRKAVVQAWMMRTTIHMASRSDFWLFSAAV
                     REHRREGWIRGFRRSASEAATAARDTARFLAEGPKRRAEIVSTLGFDNATWNGVGLWL
                     DLVRVPPFGTWEQPRADLYGLASDWLGEPPAVTQEEGTAHLVRRYLAAFGPASRADVV
                     SFTGIPVRALTAAFERIPLRRFLDERGKELLDVRRAPLPDPGTPAPIRFLPAWDATLL
                     THARRTQILPERYRARVFNTKTPHSIHTYLVEGQVAGSWRHEHGRIVTEPFERLSTKT
                     RRALDDEAAYLAELFATE"
     gene            2432..2731
                     /locus_tag="VF195_08055"
     CDS             2432..2731
                     /locus_tag="VF195_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6580810.1"
                     /translation="MQLEITATGDGYRLEGELDMATAGGLTELLLAASAGDTPLVLDF
                     SGVSFMDSSGLRALLEGAELPNGSGPVVIQDPSPQVRRVLEITIPDGVPGLQVRP"
     gene            complement(2760..3761)
                     /locus_tag="VF195_08060"
     CDS             complement(2760..3761)
                     /locus_tag="VF195_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005517358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX6580811.1"
                     /translation="MPAITVDDATALARLPEARGSSVVRPVRAVVTAPGGFEGEGFPV
                     RRAFAGVELTDLDPFVHMDQMGEVEYAPGEPKGTPWHPHRGFETVTYMLDGIFEHQDS
                     NGGGGVITNGDTQWMTAGAGILHIEKPPEHLVASGGLFHGIQLWVNLPKAEKWTPPRY
                     QDLRGSEVGLLASADAGALVRVIAGDLAGWTGPGQTYTPVTMVHTTIAPGSRLELPWN
                     PSFNALAYVLSGEGTVGPDRRPIVTGQLAVLGAGDLVRLEAASSQESRSPALEVLVLG
                     GRPIREPVAWYGPFVMNTRDELVQAFEDYQAGRLGTVPAIHHTPDHVVESDPGSEPE"
     gene            complement(3857..4054)
                     /locus_tag="VF195_08065"
     CDS             complement(3857..4054)
                     /locus_tag="VF195_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580812.1"
                     /translation="MPETFGKRQRKDVKAKKAAAAEERRLARVQRRRDREAGLIEPGP
                     PVATPEEIESQLGIVPPGLAD"
     gene            4282..4932
                     /locus_tag="VF195_08070"
     CDS             4282..4932
                     /locus_tag="VF195_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580813.1"
                     /translation="MLGSIGSRLPQRSVVAIDLLVLLWTVVWLVLGITVGTFVERLGA
                     VGESVEGAGHAIHRAGDAVGRLSEVPLVGEGFEAVANEIRTIGDETVQNGRSVEQDID
                     TLALLIGVGLAAGPTLPVLAAWIPPRVSRERERRALRRSLKSGDGVALAYLANRAVAG
                     RLFRELRTASDDPVADLAAGRYEALARLELEHLALASTSWLPRSASTAREEGSRPG"
     gene            complement(4918..5598)
                     /locus_tag="VF195_08075"
     CDS             complement(4918..5598)
                     /locus_tag="VF195_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580814.1"
                     /translation="MPVEVYVEATGKRAFAGAVDWPGWCRSGKDEEAALESLLGYGPR
                     YARILKGSGVRFTTPARPSTLSIVERMEGDATTDFGAPSIAPKADATSIDRRWLTRNE
                     TILRACWASFDRAVAGVRGELRKGPRGGGREVGAIVGHVVGAEGGYLRMIAGRPPSFV
                     EDDASAAREPVRAAVLEGLERALVDGIPEKGPRGGARWSARYFVRRDAWHVLDHAWEI
                     EDRSSAGA"
     gene            complement(5608..5823)
                     /locus_tag="VF195_08080"
     CDS             complement(5608..5823)
                     /locus_tag="VF195_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580815.1"
                     /translation="MRVTFVDGPLDGREEEIPDDTLEEGHPVYWPERPETDDDTDPGQ
                     PGLEGVVEYLYDGNGRASYVGGRLEDA"
     gene            complement(6028..6414)
                     /locus_tag="VF195_08085"
     CDS             complement(6028..6414)
                     /locus_tag="VF195_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017197765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HEX6580816.1"
                     /translation="MAPSKLPKPTDEAKDFFSSVVPDHPAVSIRPMFGQLSAFVNGNM
                     FMGIFGEDVLVRLPGADRAEVLGAGGSVFEPMAGRTMKEYVVLPGAWRDDPDRIREWA
                     ARSLDHAEELPPKQPKKKPKPKKPKA"
     gene            complement(6425..7411)
                     /locus_tag="VF195_08090"
     CDS             complement(6425..7411)
                     /locus_tag="VF195_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580817.1"
                     /translation="MRRRFVRKFVVGSAILIAAIVTFAVIVGGAIAGGPGHGRPYGFV
                     GLLIVALAVFLIARAGRRVAAPVGEVMEAADRVADGDYEVRVRERGPRDVQRLGKAFN
                     AMTERLGSSEARRRQLLADVAHELRTPLAVIQANLEALIDGLYPADETHLRSVLDETK
                     VMARLLNDLQTLSTAEAGALTLHREPTEPRHLIESAVRSFAARAGEAGVRLDTRVTES
                     LPEIEVDRLRIGEVLGNLLSNALRHTAAGGSVTLEAFPAGEGVGLSVTDTGTGIPPER
                     LPHVFDRFSRAADSPGTGLGLAIARSLIEAHGGEIRAESTTAGTTISFTLPK"
     gene            complement(7545..8228)
                     /locus_tag="VF195_08095"
     CDS             complement(7545..8228)
                     /locus_tag="VF195_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580818.1"
                     /translation="MKTVLVVEDEMKIARVVRDYLTDAGFDVTVTSSGEAALASARGS
                     RPDLVVLDLGLPGIDGLDVARELRGSSHVPIVILTARGDETDRIVGLELGADDYVVKP
                     FSPKELVARVRAVLRRSSTAEMLREQPVRVAAIEIDPQRMRVTASGRPVELTPTEFQL
                     LAALAREPGRVFTRGQLLDAIHGMAIESYERAIDAHVKNIRKKLEPEPGTPRYVLTVH
                     GVGYRFADD"
     gene            complement(8306..10117)
                     /locus_tag="VF195_08100"
     CDS             complement(8306..10117)
                     /locus_tag="VF195_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6580819.1"
                     /translation="MGAALLAVICVVSLQVLRVFFPIVFDGFEDAGYVAAGAVTVGIP
                     ALGSILGSSFREVIGDRRSVLLGVGLLAAGRIALQAAGPIPFWLVAITTAAAFWALMS
                     IVATGGFAGRAPRPVQGLLLGFAVDLAIRSVFHGWDPVWQEGVLPWVVAVALPLGAMT
                     LAIVTSRTVRSDGSSTSWVPLVAIGPFLFLHAVFLQNVPFVASRSGVTSLQAVAVILA
                     LDAATIFVVGAWPRWQIPLAVMAAVGMVGLRLGPGAGVLTLFALATAGTAAMLVLALR
                     SSAGGGRVSRLLAHAGGPALLGALVVAYQKPRPLPLPGGYLLEAATITLLLLAWVGSR
                     SPPGRLAPLGRRAAFGATLLVGVPLGLWLSTPAPATRSTSAPREIRLLDYNVHSAVND
                     GQVDLEALASTIEAQRPSIVVLQEAGRGWPVTGMTDVVEWLSWRLRMPYRFGPAGDGP
                     YGNTIMYRPTLRVLATERGSLPQGGGLHPRSYLSVTFAQDPLRVVATHLDGEEDDGTR
                     LIQIDALLRAIDDADGTVVAGDLNAEIGSPELAKFQDFGFVTVQDLTGEWAATFPEEG
                     EIFDHILIGSHLTATDVAVGRTDVSDHFPVVATIRRR"
     gene            complement(10196..10576)
                     /locus_tag="VF195_08105"
     CDS             complement(10196..10576)
                     /locus_tag="VF195_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580820.1"
                     /translation="MRRGFGWGAAILVVLLLVGVGIGAYNWGVTEGLERTGEGVEVVR
                     YVGGHGFGFFPFFLFFPLFFLALFAFKGAMWRRHWDHDGGPGEHHGPGGHGPGEQGYE
                     EWHRRQHESGGGWGGQGPPEQREN"
     gene            10686..11222
                     /locus_tag="VF195_08110"
                     /pseudo
     CDS             10686..11222
                     /locus_tag="VF195_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
     gene            complement(11266..12192)
                     /locus_tag="VF195_08115"
     CDS             complement(11266..12192)
                     /locus_tag="VF195_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX6580821.1"
                     /translation="MELRTIGQIPVPLAGIGCNNFGRRIDEERTREVVDAAFEADATL
                     FDTADLYGDGHSEEFLGRALGSRRDRAVIVSKFGMRTPSDGMTGGDPRWVVRACEDSL
                     RRLGTDRIDVYLLHTPDDATPIGDTLTAMSRMVDEGKVREIGCSNFSAKQLDEAEEAA
                     SELGIRSFVTVQNEYSLLERGPRDEVLSTCRRLGLSFMPYFPLASGMLTGKYQRGEPP
                     SPGTRMAGRGDWDEFLTDERFDVVELLSRFAEAHGHTLLELAMSWLSVQPEIATVIAG
                     ATSAEQVRQNAEAIEAWRLTDDERAEIDELLT"
     gene            complement(12214..13800)
                     /gene="cimA"
                     /locus_tag="VF195_08120"
     CDS             complement(12214..13800)
                     /gene="cimA"
                     /locus_tag="VF195_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017658766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HEX6580822.1"
                     /translation="MTGPRVELYDTTLRDGAQAPGLSYSVEDRMRILHKLDQLGLPYI
                     EGGWPGANPRDTEFFRLATKETLDHAELVAFGMTRRAGEEAEDSAILRDLLDAGTETV
                     TFVGKSWDVHVEKALRTDLEEGVAMVRDTVRHLRAQGRRVFFDAEHFFDGYLGNADFA
                     IRVLEAAAEEGAERLVLCDTNGGTLPSDVARIVGEVRARVDGVPLGIHTHNDAGCAVA
                     SALAGVEAGVLQVQGVVNGYGERTGNADIVPIAANLVLKMDADCLPPGAVERLTEVAH
                     YVAEVANVAPDSRQPYAGRFAFTHKAGLHASGVARFSEAYEHVSPDAVGNRRGVVASD
                     LGGATTLRMKAAEFDFAIEDEAVPVVLEQLKEREARGYTFELADASLELLMRRASGWT
                     QPFFEIDSYRVHVEERVGDGEAPLAEATVKVRTKGSRHIESAEGHGPVGALDDALRKA
                     LTNDYPELRHIELEDYRVRVLAETHGTGAVVRVLIDSSNGEHSWTTVGVSENIIEASW
                     EALTDAYLFGLLHPREIAEP"
     gene            complement(13797..14900)
                     /locus_tag="VF195_08125"
     CDS             complement(13797..14900)
                     /locus_tag="VF195_08125"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012546078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX6580823.1"
                     /translation="MSASVATEVQPERVHSDSRPYRIGVIRGDGTGPEVVAEALKVLG
                     AVRGDVTIELVEFDLGADRYLRTGETLPDEELETMRGLDAILLGAVGDPRVPPGVLER
                     DLLLRIRFELDQYVNLRPVIRFPGVQTLVPSHTEDDVNMVVIRENSEGLYSGVGGFHR
                     KGTRHEVAVQSSVNTRLGVERIVRFAFDHAMLPGRRRHLTLVHKTNVLTFAGDLYQRV
                     VNEIAPEYPDVTTDYVHVDAACIYFLDQPSRFDVIVTDNMFGDIITDLGAMIQGGMGI
                     AAGGNINPSGVSMFEPIGGTAPSETGKGTINPLAAIGATGMLLAQLGEERAAHRIDAG
                     IRFACTKLASMRAGEMGYSTREVGDLVVEAVSS"
     gene            complement(14998..16608)
                     /locus_tag="VF195_08130"
     CDS             complement(14998..16608)
                     /locus_tag="VF195_08130"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX6580824.1"
                     /translation="MAAERGQGAAVVSDPDVVRFFDTTLRDGEQAPGIALTKDEKVEI
                     AEQLARLQVDILEAGFAISSDGEFEAVREIATSVRGPVIASLARVVPGDIERAAEALK
                     GADRFRIHVFISTSQIHMEDMLRMSHRQVLDGIEEGVKLAAGYTEDVEFSAQDATRTE
                     LDFLLECFDVAVNAGATTINVPDTVGYATPAEFGELVRIVRDRTPEHVIISTHCHNDL
                     GLAVANSLAGVENGARQVEVAVNGIGERAGNCSLEEMAMILRTRREALGVRHGLNLKE
                     IVRTSRLVSALTGYSVQPNKAVVGASAFAHESGIHQHGVLANRATYEIMDPVEIGLEG
                     NQIVLGKHSGRHAFADALDKVGISIDDDHMHRAFARFKELADRKIQITDQDLAAIVSE
                     EVGSGKEDLLVLESLASGGGTHLAPTATVRLRRGDEVIEESAMGDGMIDAACGAIQRA
                     AGVEAKLITFNVSSVTGGSDALGDVVVQLEIDGRRVTGRGVATDVVEASARAYLAAVN
                     RAAMVADASIAASGGETAMSEPNTEVTP"
     gene            complement(16572..17585)
                     /gene="ilvC"
                     /locus_tag="VF195_08135"
     CDS             complement(16572..17585)
                     /gene="ilvC"
                     /locus_tag="VF195_08135"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX6580825.1"
                     /translation="MATIYHEQDTDVSALAGQKIAILGFGSQGHAHAQNLKDSGYDVR
                     VGLRPGSSSRTKAEEAGLRAVDTAEAVADADVVMVLLPDTSHGKVYAEEIAPHLNAGD
                     MLMFAHGFSIHFGTVTPPPDVDVTMIAPKGPGHLVRRTYEEGIGTPALVAIHHDATGK
                     ARQRALAYGHGIGAARAGIIETTFKEETETDLFGEQTVLCGGLSELIRSGFDTLVAAG
                     YQPEIAYFECLHEVKLIVDLIYEGGLSWMRHSISDTAEWGDYVSGARVIDDGTRETMR
                     QLLAEIQDGSFAKRWIAEADGGFPEFLAKRREARASQLEDVGRQLRTMMPWLRSEDKV
                     PPS"
     gene            complement(17611..18174)
                     /gene="ilvN"
                     /locus_tag="VF195_08140"
     CDS             complement(17611..18174)
                     /gene="ilvN"
                     /locus_tag="VF195_08140"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX6580826.1"
                     /translation="MSLHTISVLVEDKPGVLTRVAGLFSARGYNIDSLAVGPTEAEGL
                     SRMTIVVNVESKSLEQVTKQLNKLINVIKILEHEPGSAVERELMLAKVRAKGDARARV
                     MEIAEVFRVSIVDVTKTTLTVEASGKPEKLEALRELLSEFGIVELSRTGRIALARGDR
                     GIREKSGLRVAKAVGAEDSPEWTGGGV"
     gene            complement(18171..19916)
                     /locus_tag="VF195_08145"
     CDS             complement(18171..19916)
                     /locus_tag="VF195_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018178952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEX6580827.1"
                     /translation="MKVSGAQALFKALEGEGVDIVFGIPGGAILPAYDPLIESGVRHV
                     LCRHEQGAGHAAEGYAWATGKVGVAIATSGPGGCNLVTALADAKMDSVPIVAITGQVP
                     MPAVGNDAFQEADITGITMPVTKHNFLVTDPGEVAETIREAFHIARTGRPGPVLVDLP
                     KDVLVNETTWSWPDQVDLPGYRPTTKGNMRQVVEATKLILEAKRPVLYVGGGVIKAEA
                     TEELYELATVGHLPVVTTLMARGAFPDAHELCLGMPGMHGVYTAVTAMQKADLLVAVG
                     ARFDDRVTGKLATFAPDAKIVHIDIDPAEIGKNRRADVPIVGDCRDVTAKLVAELTRR
                     QDEAGIPDRSPWLEQLATWKAEQPLRYDQEPDGPLKPQYAIERLRAHSDGDTIVVAGV
                     GQHQMWASQFWSFEKPRTWINSGGLGTMGFAVPAALGAAAGQPDRRVIAIDGDGCFQM
                     TSQELATSTTEKIPFVTAIINNANLGMVRQWQELFYEERYSQVFLGYDVPDYVKLAEA
                     YGAVGLRAERPEEVDAVIEKALSVDDRSVVIDFRCDANEMCFPMVPAGASNDDIITGP
                     EGLQPQDDPAEAPIA"
     gene            complement(19913..20209)
                     /locus_tag="VF195_08150"
     CDS             complement(19913..20209)
                     /locus_tag="VF195_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580828.1"
                     /translation="MRYEFEKDGVTVASVLWEGPGQVSVETDDPSTRTTLEGYLSSEV
                     TYLSGFGGEELQSRRRDWTPWEFERACRNLARRLGATVKRVETGPVEERQAVAG"
     gene            20473..20703
                     /locus_tag="VF195_08155"
     CDS             20473..20703
                     /locus_tag="VF195_08155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580829.1"
                     /translation="MEPLPDEESPVPRVHPLEIPFVWFGLQLVEYAVVWLLITTFLPE
                     TMPGPVGWTLLLVLVIGLTILNYRIRRRFIPR"
     gene            complement(20716..21054)
                     /locus_tag="VF195_08160"
     CDS             complement(20716..21054)
                     /locus_tag="VF195_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580830.1"
                     /translation="MSVVKSLWASVQGDPVFMRRLNGWLTIFWILMIPTSILTGWISS
                     VTYVAALSLWALVSGHWSAWQAARVEVEQAKEAEKREKEKQDLPSEIVERILEQTDLQ
                     RVRAASSSAG"
     gene            complement(21116..22510)
                     /locus_tag="VF195_08165"
     CDS             complement(21116..22510)
                     /locus_tag="VF195_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017973285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX6580831.1"
                     /translation="MSDRVSSGSERLNDMLGGGLPDNGINLIIGLPGSGKTILAQQYA
                     FHNATRERPAIYLSTVSEPLEKILRFGQTLSFFDRGAIGRSVFYEDLGGTVNDEGLQG
                     VLERISAFLKERRPGMIIIDSFKALHPYARRDEEFRRFLHELAGRLSAFPVTSFWVGE
                     YDQAEIAGAAEFAVADSILSLSVERVGGRELRVFQVLKLRGSDFLSGKHAYRLSGDGV
                     QFFPRLADPVDQAPYELEDVKISSGIKILDEMLGRGYWPGASTLVAGPSGSGKTLMGL
                     HFIFEGARGGEPGVIATLQENAAQLERVANGFGWSLDEEGVGLMYRTPVDLYLDEWVH
                     ELLGTVEATGARRIMIDSLGDLQAASTDQIRFREYMYSLLQRCSRQGVSVMMTQEVGD
                     LFGVSRLSEFGISHLSDNVVLLQFLRGESRVKRGITVMKTRASAHDAQIREFEISADG
                     FVVGDHFAADQDLA"
     gene            complement(22507..23157)
                     /locus_tag="VF195_08170"
     CDS             complement(22507..23157)
                     /locus_tag="VF195_08170"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6580832.1"
                     /translation="MTAAIYSIGAVARMLGVPTATLRTWEERYGVVMPDRSPGGHRLY
                     TRDHVEQLRFVLRSIEQGMQPADAHRLLTDRLGDDPDAQVAITGSETRLVIMLAERDP
                     HAAELAEYFLRTEGYETIMALEAREAEAIFEEQSPHLAVVDLLISGGAGPDLCRTFRE
                     RGALVLAISPLESRDEALEYGADAFLQKPLDPLQLVSTVKDLLGQSAFLRTEVLSP"
     gene            23342..25156
                     /locus_tag="VF195_08175"
     CDS             23342..25156
                     /locus_tag="VF195_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX6580833.1"
                     /translation="MQESREREHDSARTTSERLVKENRRYDPGTGGGGSNNNGASAEA
                     RYRALFESAGDAILIVDREGLCIDANPRATELLGYERRELLSMPVLDLVAAEPGWADE
                     EFRHLIDQERWTGELELRREDGSLVPVDVSATRVDIGSGPAIYLAAMRDLSDRKQLIE
                     ERAELVSAEAEAQTQAEAARQRVRFVSDVSELLAVSLDYPATLVRLAEVVVREIADLC
                     VIDLVQESGTFERVAVVHASSAKQALAEQLGGYAPRPGSSDPVARVFATGEPQVFSDQ
                     SPGSLRAITQDEGHLRIARELGVQSFLSVPLIARGRTLGVISLVSTNPDLRYGESDVR
                     LAEEIARRAAVRIDNARLYDERDHTAKVLQRSLLPPSLPSIPHVELAARYLPVGEGNE
                     VGGDFYDVFDCGDGNWAAAVGDVCGKGPEAAAVMGLARHTLRAVARFERKPSALLREL
                     NRALLEQSVEERFCTVCHLRLRPSRDHLRITLCSAGHPLPLLLRRNGTLSSVGESGQL
                     LGVFPEVSLVDSVVDMDPGDTLLIYTDGATEQRREAVAGEERLRDAFAASLGRDAEAT
                     TAGVEEALRAARGSSPQRDDVALLVIRAAESDPRPSES"
     gene            25207..26127
                     /locus_tag="VF195_08180"
     CDS             25207..26127
                     /locus_tag="VF195_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase family protein"
                     /protein_id="HEX6580834.1"
                     /translation="MVTDVSDPALDAVIHAIPSWEGKELEIIPIAEGRTNRNFRVTAG
                     DDAFFLRLSEKSTELLGIDRTAEQDSVRAAAQAGVGPEVFVYLPELGCLVTRWIDAEA
                     LSEGDLERQEILAPVVDAIQAIHAAPQLSWSFNPFRIVEDYRRISEERGVIVPDAFDG
                     AHEKASRIEAAFTSDPMPLVPCHNDLLEANFLLRDGHVWVVDHEYAGMGDPFFDLGNL
                     SINNALSDDAQERLLTLYFGEPNDAHRARLKLMRIMSDFREAMWGVVQQGLSTLDIDY
                     VEYATKHFDRLSTTMADPRFDDWLETARGI"
     gene            26131..28560
                     /locus_tag="VF195_08185"
     CDS             26131..28560
                     /locus_tag="VF195_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020547985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6580835.1"
                     /translation="MADDRARVVVIGGGVAGTSIAYHLTRLGWTDVLLVDRGELTSGS
                     TFHSAGLVAQLRNSVSHTRMMMYGAELYPTLAEETGVDPGWHQVGTLHLASSPYRMEA
                     LARQAGWAKSFGLPVEIVSTEEAVERFPLIDASRVLGAQFVPTDGHIDPTGLTLSFAA
                     GAKAAGGRVRTGERVEEITVRDGRVAGVVTDHGAIEADVVVNAGGIWAHELGRLAGVE
                     IPVVPMEHQYLITRPIEGVRADFPTLRDPDNLVYVREEVGGLVVGGYERNPDPWHVSD
                     PIPRDFNHRLLPEKWERFESIADGAFRLIPPLQTIEINRFINGPEAFTPDDDFILGET
                     EVAGFFVAAGFCAHGVTGAAGIGRWTAEWIVEGEPSIDLSKMDVRRFGRHYASRDYAL
                     AKAYEIYAKHYDVRYPGEENEAGRPLRMAPTYSRLEKLDAEFGEKAGWERANWFRSNE
                     DPAHEERRPRGWAGEQWSTAIVAEHLAVRARAALFDETSFAKFEVSGPGALDLLQRLC
                     ANDVDVRVGRIVYTQMLNRRGGIESDLTVTRLADDRFRLVTGTASGSRDLAWIRRHQE
                     GHDAHVRDVTSGLACLGIWGPAAREILASVCDDDLSNEGFRYLTARDIEVADVPCFAA
                     RVTYVGELGWELYPSTEFAVRLWDRLIEAGAPHGLLPAGYRAIDSLRLEKGYRSWGAD
                     ITPDDSPLEGGLGFAVAFDKDADFLGRDAVRRLRDEGVTRRLRCLTLADPRSSTLGNE
                     PVRAGDEIVARVTSGGLGYTVGKSIAFAYLPDGLDGLGTKVSIEVFGEWVDAEVVDDT
                     LWDPSGERIRA"
     gene            28560..29324
                     /locus_tag="VF195_08190"
     CDS             28560..29324
                     /locus_tag="VF195_08190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020736610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX6580836.1"
                     /translation="MEQPIFELDGQVALVTGAGRGIGRDLVVALTGAGARVAAGIRSP
                     GDGGSVVDEARVGGGDAEAVTLDVTDRGSIERAVEETVERFGRIDILVNNAGLGTNHD
                     ALDATEEEWDELFAVNVRGLFFASQSAGRRMVEQRHGRIINMASQAGLVGIPRHAAYS
                     ASKGAVIALTKVLALEWAPFGVTVNSVAPTFIRTPGTAERLDSPEFLADVMERLPVGR
                     VGTTTDVAGAVIYLASPAASLVTGTVLVVDGGWTAQ"
     gene            complement(29329..29685)
                     /locus_tag="VF195_08195"
     CDS             complement(29329..29685)
                     /locus_tag="VF195_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580837.1"
                     /translation="MKLVVSERAAAYIAERGGAAWVWLDPHRGLVGSHIYLEAHAEPP
                     RSSRRTSFTRSSRRPHRFEAMTAEGITLHYDFGRMEPPAELHLDRKGWRAGTHRLEAY
                     WNGSIFAGDDIPAPGA"
     gene            complement(29741..31381)
                     /locus_tag="VF195_08200"
     CDS             complement(29741..31381)
                     /locus_tag="VF195_08200"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6580838.1"
                     /translation="MSVHGDQVARDTADLERFGYKQELKRELHTFSSFAVAFSYISPS
                     TGIFTLFFLGMAALGGFLFWTWPVVAAMQFVVALNFAELSSHFPVAGSVYQWTKYLAN
                     RGYAWFTGWFYLIAGILTVASVCATLPLALFPMLNIMFGWNLTADFSTGGHVDQAIAA
                     LVTLALITILNIYGVKLVAIVNNTGVFFEVLGMVVFAIFLAVTQNNSGAGVIVDSAKT
                     GNIFQFPADVTLGFFLVGMFMSLYVIYGFDTASTLSEETHNPRQEAPKAVLASVIGAF
                     VIGAVFLWGVLIAVPDMGEAVAGFFGPTTIIDAVLSDALTVIYLLVVVASIFVCCMAI
                     LTSTIRLAFGMARDDQLPFSKSMAKVNPRLHTPVATCIIVGALSAIPFIQFAGASTIA
                     VGATASIYFSYLLGNLAFMRARARGWPKTRAPFSLGSWGKVVNVVAILWGAAMLLNFL
                     TPSSATSAFDPNASDANYLRIFSNPKPIQTDYFVEGEQLVDFKIGFLNEIPVIWTVFA
                     LVSIIGAIYYFAVQRNKPFVPVRPPEEEDLAGIAPAEA"
     gene            complement(31486..32724)
                     /gene="thrC"
                     /locus_tag="VF195_08205"
     CDS             complement(31486..32724)
                     /gene="thrC"
                     /locus_tag="VF195_08205"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX6580839.1"
                     /translation="MTAAITSPAALQCRMCGARYNVEPLTICEECFGPLEPSYDLDAI
                     DGVDFRKQVEAGPASLWRYELLLPGGPGLDRVDLGAGFTPLRRADRLAAELGLRDLWI
                     KDDSVNPSYSFKDRVVSVAATMARAFAFEALSCASTGNLANAAAAHAAHAGMPCYVFV
                     PDDLERAKILATETYGAKVVAVKGNYDDVNRLCAEVADTLPWAFVNVNMRPYYAEGSK
                     TLGFEVAEQLGWRVPDHVLVPIASGALLTKVHRAFQELMAIGAVEEKAYRVSGAQPEG
                     CRPVAQAFQDGAEEVTPVKPDTIARSLAIGSPADGYFALQVARETGGAIEWCTEAEIL
                     EGIRLLASTEGVFTETAGGVTIANLKRLVEQGVIGPDEEVVAFVTGNGFKTVEAFEGS
                     VEPSFHVAPDLDEFLAALAR"
     gene            complement(32747..34429)
                     /gene="ilvD"
                     /locus_tag="VF195_08210"
     CDS             complement(32747..34429)
                     /gene="ilvD"
                     /locus_tag="VF195_08210"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEX6580840.1"
                     /translation="MEREHDRPRSWEVTEGAHRAPARAMLRAVGFSEDDFGKPQVAVA
                     SSWNEVTPCNYHLGKLAALAKEGVRQGGSVPVEFGTIAVSDAIAMGHEGMKASLMSRD
                     LIADSVELVMHAERFDALVGIAGCDKSEPAMMMVMARLNLPSVYLYGGTILPGTYGGR
                     EITIQDVFEAVGAHAKGTIDESELLGIERAACPGTGSCAGMYTANTMAAVGEAVGLSL
                     PGAAGPPAVDYRREVLARESGIRAAELVAAGGPLPRDILTKEAFENAITVVMALAGST
                     NAVLHLLAIAREAEVDLQLDDFDRISRATPHLVDVRPAGKFVMSDLDRVGGVPVVMKE
                     LLDLGLLHGDCLTVTGRTQAENLAEVAPPAPDGDVVHPGTNAIHPWGGIAILRGNLAP
                     NGAVVKSAGAEGLVFEGVARPFDSEQDAFAAVTSGDIVAGDVIVVRYEGPKGSPGMPE
                     MLAVTAAVAGAGLGKDVALITDGRFSGATKGYSVGHIAPEAFIGGPIALVQNGDRIRI
                     DADARSLELLVDDDTLATRHAAWVAPEPRYTKGALAKYAKLVGGAEHGAITG"
     gene            34497..35909
                     /locus_tag="VF195_08215"
     CDS             34497..35909
                     /locus_tag="VF195_08215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6580841.1"
                     /translation="MTTVPPDAGRSWWLREALADDPGEPASPLRGDLTTDVVILGGGY
                     TGMWTALHLKELDPGIDVAIVEQDIVGGGASGRNGGFVNSFWGDLDYLAHHFGDRATI
                     ALCEAGEESVKAIGRFASDQGIDAWYRADGEYVVAASDEQFGRWADGLITADRLGVSQ
                     HFQVLSADELRERVASPVFRGALFRTVGATVHPGRLARGMRRVLLERGVRIFEDSPVT
                     RFGAGDPARAETPGGTVQAGVAVVALNAWAHHWKRFRRTVVVRGSYIVMTEPAPDRLK
                     EIGWTDGSGVADMRSAVHYVRTTPDGRISFGLGGVQPNLARRIGPRFAYDPRAIEIAI
                     ADLHRMFPGFVDVPIEAAWGGPIDVSGHHLPTFGTLGKGNVHYGHGYTGNGVGPAHLG
                     GRIMANRILGKADPVLDLPLVDLEPLRFPPEPVRSPGALVANTAIRRKDEAEDDGEEP
                     NPLIDFVARLPRRLGYNLGP"
     gene            complement(35906..37294)
                     /locus_tag="VF195_08220"
     CDS             complement(35906..37294)
                     /locus_tag="VF195_08220"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6580842.1"
                     /translation="MLEELSLAVRDGQLSAEELVRTSLERIERLNPPLNAVISVREQA
                     VDDARALDARVTAGEEVGPLAGIPLLVKDMEDVEGVPSTYGSVVFADAPAAAADGLIP
                     RRLKAAGAIVVGKTNQPEFAFAGFTDNLLYGPTRNPWNPEASPGGSSGGSGAAMSAGI
                     APIATATDGGGSIRIPAGWSGLVGLKPTNGVVGRQPIPEWIDLSTFGPLGHTVTDVRL
                     LLGIEAGPVPGDPTALPMPLEMVAEMPTRAFASPRLVDYGPLPESVQELFDVAVASFE
                     RDVGVPVERIDADRIFRAGHVSEDWLLICAMEHVHHFGMDFCEENLERFSQVFRGVVE
                     FARRASFEDYMAARRRRFAYVRELDLLLGDDAVLLTPTNCVAWIRADGTNPETGEVGD
                     SPDAFNTDPQNMTGHPAISVPAGVTPDGVPFGLEITAPRFRDGMALTAAEAWERTNPW
                     PLVAPGYEPFGA"
     gene            complement(37353..38213)
                     /locus_tag="VF195_08225"
     CDS             complement(37353..38213)
                     /locus_tag="VF195_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580843.1"
                     /translation="MSDVPGGPTPPTPPPPPPMMGGPQGGIPPKTVGDILSAAFNIYK
                     ANASQLILVVAVVVVPLSLIAALFSGVVFAPERIRVTNTGGVVAFDYAGRGLGVLLLV
                     GAIGALIAVLISAILQAAILRAAAQATIGDPVDVEASYRYGLKRLWSVILVSLLVGLI
                     VAVGFVLLVIPGIIFLVLLSVSIPVLIVEGRRGTEAMGRSWNLVKGHFWHALGVIVVA
                     ALIVGIISGVIGGIGGDNWAVRWIFTAIAQILTVPFASLVSVLLYLDLRARSEALFAE
                     TLRRELASGT"
     gene            38269..38607
                     /locus_tag="VF195_08230"
     CDS             38269..38607
                     /locus_tag="VF195_08230"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580844.1"
                     /translation="MPITSTRSSTEEHSCHGPSTLGTLRSHESLVLDVRLDRRDAGAV
                     AQFWEAVTGHPKEQVHQPGNDHWVVSPPDGTLPRLVFVTVPQEKSIKDRMHLDLLANH
                     SIQEDEVALG"
     gene            38644..39822
                     /locus_tag="VF195_08235"
     CDS             38644..39822
                     /locus_tag="VF195_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6580845.1"
                     /translation="MDLRLSAADRDLQQKARDFTDQVLVPLEDECEANDGLTPESHAA
                     AKQAVLDWDFHAINHAEADGGRGHDLFAQMLIEEQWGRATGALWDIPWRPSIPLAGGT
                     EEQKERFLRPACRGERRDAYAITEEGAGSDPTMVETTAGRDGDDWVLEGEKWHVTSGD
                     VADFFLLHTHVDGDPAKATIFLVDKDAPGVRLVRTPKYMHTFVFEHPIFAFESVRVGG
                     DRILGQIGRGFELTKDWFVEERLMIGARTVGAATRALELSLDFAKDRRQFGRPIVDFQ
                     AVEFMLADMAAELMAAKSMLYRVCWHAGRGGADRKELHAMAAAVKLVCSETAGRVADK
                     AVQIHGGRGYMREHPVERLWRELRVDRIWEGTSEIQRVVIGNELRKRGAGRYTGWPTE
                     "
     gene            complement(39824..40189)
                     /locus_tag="VF195_08240"
     CDS             complement(39824..40189)
                     /locus_tag="VF195_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580846.1"
                     /translation="MAGVFRSSGRHTNPLGAISVVTGLAAALASAVLWIYRVQPDSKI
                     LGQYSAEVAAGGQLADQLVALAAILGLMAVLSSIMTSSSGEGGGSYFMGLVLGLVGLS
                     YPLLDWLNVMSEPLRPNIL"
     gene            complement(40189..40839)
                     /locus_tag="VF195_08245"
     CDS             complement(40189..40839)
                     /locus_tag="VF195_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580847.1"
                     /translation="MTKRMIAVALLLFLVACEGEPEPFPPPPLPTSASPFPSTPTLAS
                     PTPPALPPDPLPTSGDASETCAEGWVSPGADSALFEKPLRIIRRSVQLPGEPTVVEMR
                     YFTGPESPPSDKGYLSVVERWYVKLYTEDDLRFQGRFLVESREFGDGVAAVAPYDTEG
                     FRSPDWSGFQWADTEPSPYPGLPGEWSGTRYDFVKGGEGLDIPGLPTEVRGCLDTA"
     gene            40895..42307
                     /locus_tag="VF195_08250"
     CDS             40895..42307
                     /locus_tag="VF195_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6580848.1"
                     /translation="MDTGPYGHTRDERHPMPLSRRRFLRTSAMAVAGGVLFSCTGGTR
                     IPKVDDTIPETDTRWPIKRVMYVMLENRSFNNLFGKFPGVRGGTTIGVENGVEKPLIR
                     CPEWLPGDIPHDLAAHRACVNNGKMDGFGTGIYGSTYGYTIFDEEQIPNYWHWAREYS
                     LSDTFFASAAGPSYPNHFYFVAGSSGGVTDNPENIRTRPLEEGRLFKSWGCDAVGDDV
                     FVLVTDEAGNVTKHNTCFEFPTVGEQLTRAGVDWAYYSAVPGQPGYFWNAYNGISNLI
                     HNPDLWHQHVRSVDTLLDDIDASALPPVTWVTPRFQLSDHPPFSTGHAHNWVTEIVNK
                     VMRSDMWEHTAIFLTWDEWGGFYDPVVPPSVDHLGLGIRVPLLTISPYTRRGVIDDER
                     GEFSTPLRFISDNWGLEPLTDRIRDTHNLEHVFDFAGGPRAPELGTERAPTFSPSPWV
                     FPRDTYTGWEPGTEPVEEPL"
     gene            complement(42311..42457)
                     /locus_tag="VF195_08255"
     CDS             complement(42311..42457)
                     /locus_tag="VF195_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016826775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580849.1"
                     /translation="MKRERIEKPKPRRPRELEMILPLDPRDPDVVRAKKGWVWRYADR
                     RWAV"
     gene            complement(42626..44728)
                     /locus_tag="VF195_08260"
     CDS             complement(42626..44728)
                     /locus_tag="VF195_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX6580850.1"
                     /translation="MTEDERTPLQRMLEARSVALVGASVKEGSLGLQMLLELHRGGYD
                     GAIYPVNPGYDEVLGHPCVPSIGEIPEPVDLAIIGVANQRIEQAVREAGHAGAGSVVT
                     FSSLYEEEPPEPGMAPLGERVAAIAREHGMALCGGNGMGFVHADANLRATGFLTPDDL
                     RSGGVTFISHSGSGWAAFLFNDRQIRFNLLVSSGQEIVTTMDEYLSYALDLDSTEVVA
                     LLLETVRQPDGFVAALAKAAERGVPVFALKVGRTEGSKQMVVAHSGALAGEHGAYEAV
                     FEAYGVHECRDLEEMADALELFSSPRRVTDGTGIASLHDSGGERVLFVDLATDTGVPL
                     AAISEDTKTKIADVLDPGLVAENPLDAWGTGIDADRIYVESFQALHDDPETAAVAFVV
                     DLTRQGEPYDVGYLQVARDMWERTTKPFCVISNLPATIADDEVGILREAGIPVLEGTV
                     TGLRALKHLLADATGRSRLAVDPAPTSSPGVEAEWRGRLAEAGDFSEVAALGLLAAYD
                     IPVVDALPARSLDEAEEAADRINYPVAIKTAVPGISHKSEVRGVRVGIMDVGGLRETY
                     RDLADRLGPEVTVAAMAPPGVEVALGIVRDPTFGPLVLAAAGGVLVEVLRDRKLALPP
                     IGVDAARRLIDGLAIRPLLDGVRGAPRCDVDALADAVSRLSLLAIDLGDQIAELDVNP
                     VIVSPSGCVAVDALVIPA"
     gene            complement(44725..46347)
                     /locus_tag="VF195_08265"
     CDS             complement(44725..46347)
                     /locus_tag="VF195_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX6580851.1"
                     /translation="MATNTYDAIVIGGGHNGLTAGAYFARHGAKTVVLEARNKTGGAA
                     DTSAPFADHPEINVTTYSYVMSLMPPTIINELQLKKHGYDVTPFGPYFQAFPDGRAIM
                     VYADEAERSRDSIAQFSKKDADALPEWEAWLKGVADILGPLLLQSPPRAGSLAIGDLL
                     ETARTAWKVRKLGVRGVGDVTRLFTMSITDLLDDWFESDAIKGMLAVNGIIGTWAGPD
                     EPGTAYVMLHHSIGDVGDGHLGSWGYQQGGMGAVSDSIRRSAEAFGAEVRTNARVKSM
                     LTSGGRFHGVVLEDGTELRAPIGVTCVHPKIGFLDMIPPTDLPADFVTDIQRWKTRSG
                     VVKINVAMSELPDFTAMPGKELQEHHTGSVELCFSPRYAERAFQDSHMDRKASVAPFV
                     DGVIPTTMDKKLAPEGTHVFSMFSQWVPDDWVNEPHREELDAYAKRIFDLYDELAPNF
                     KDAIIDYQVIGPYDMEQELGLIGGNIFHGELSVDQLFHMRPAPGYADFRTPIKGLYHG
                     SSATHAGGGVNGIPGWKAYRAAVKDRALKKRS"
     gene            46547..47197
                     /locus_tag="VF195_08270"
     CDS             46547..47197
                     /locus_tag="VF195_08270"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6580852.1"
                     /translation="MAKERAKAKPGVKVGKDRRQEILAAAAQVITDRGLAETRIQDIA
                     ETCGVSTGLILYYFESKDRLLVEALTYANDQFYLRLSRELRKNPSARAQLDRLIELSV
                     PGLLPEFSFLDEWALWVEIWVRALRDPEMAKEHEVLNRRWRRSIADIVRHGRSTGEFP
                     EGLDADELGIQIGAVVDGLAIQVLMDHGDVTPENMLRVSREVSAKLIGFALEPAGA"
     gene            47238..48236
                     /locus_tag="VF195_08275"
     CDS             47238..48236
                     /locus_tag="VF195_08275"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX6580853.1"
                     /translation="MAPRPTILDVDTGVDDALALLYAVASPEVELIGATSVMGNITVE
                     QATENTLAVLEAAGRGDVEVAQGAARPLGRDHVPFPVVHGERGLGRAAPPPPAAEPST
                     RSAVEMIVGSARERPGEVLLIATGPLTNVAFALREEPHLPDLLGGFALMGGAFARGGN
                     VTPAAEANIWMDPLAADEVFTAFSGADDARLPICVGLDVTERAVMRRPDLDAVCAPAP
                     DSPVGQLIEGATSFYMDFYAAVVGEDGCRLHDPLAVAIAIDESLAQLMTTRVEVETEG
                     RWTMGETVADLAGVRGSPWPVGWEPEANARVALEVAAEGFMERFVERVRSLVEARA"
     gene            48233..49105
                     /locus_tag="VF195_08280"
     CDS             48233..49105
                     /locus_tag="VF195_08280"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017983560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX6580854.1"
                     /translation="MTASVVVIGAINVDLVISGAPLPAPGQTVTGGVFAQHHGGKGGN
                     QAVAASRALGGQGVVLVGAVGNDALGHDALEALRAEGVATSVAMRPGVPTGVALICVG
                     PDGENQISVAPGANAGLTAADVTSALGQVHEIGVVLASLEVSQAAVRSAAAWCSEHET
                     PLVVNPAPVQPWVADMALHAAYVTPNEHELEALGSLPDGVVVVETRGPEGVLIRRGGE
                     ETRVEAPKVETVDTTGAGDCFNGVFAAGLAEGMDVTDAAERGAAAASLSVTMVGAREG
                     MPTHAELDAFLRTT"
     gene            49167..49937
                     /locus_tag="VF195_08285"
     CDS             49167..49937
                     /locus_tag="VF195_08285"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX6580855.1"
                     /translation="MNVVVLTKWVPEPEGTPSLGDDGLLVREGADGALDPGDEYGLEA
                     ALQLVEAAGDGEITLLTMGPEIAQAALQRGLAMGAHKGMRITDDSLRGADSLVTARVL
                     AAAIERQPFDLVIGGVESTDGYTGIVPAAIAELLGLPSLTFARELHLSDDTLTIERQT
                     AAGYDVVVAPIPVVITVTAGATEPRYPSLKGVMAAKSKPVETLTISDLGLSGDDVTAT
                     QTVAEVNDAPPKQGGETVEFDETTAGRIADLLAEAKVI"
     gene            49939..50901
                     /locus_tag="VF195_08290"
     CDS             49939..50901
                     /locus_tag="VF195_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEX6580856.1"
                     /translation="MKIWVWADVTQDGPAPSALELLTKARELGDDVAAVALGPGATAS
                     ADALGEHGATKVFASDDEVFSNHLAQPAVHALAGLVEEHSPDLILFPASYDARDVAGR
                     LHARIGSTLMANATDLFGADTARTEILGGTKVVDVELKGPTPRLVIVRPQSFAAEPSG
                     GSAEIVEVKVDVPDELTTAERTERHEEAASGPRLESAKVVVAGGRGLGGPDAFAQLGT
                     LAAAIGNAAVGASRAAVDAGWVPYSYQIGQTGKTVKPEVYICAGISGALQHLVGMKGA
                     KRIVAITKDPDAPILKIADLGVVGDLFQVVPALTEEIEKRRTGS"
     gene            50940..52829
                     /locus_tag="VF195_08295"
     CDS             50940..52829
                     /locus_tag="VF195_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012784145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX6580857.1"
                     /translation="MLFVRRVRPRVALVANARPAGRGAPTADRVTRELTHVLLQTKLL
                     QKLGPGLMHAFIFWGFIVLLPTIAEAAVAIVDKDSSLPLLGGASWFAFLSDVFATLVV
                     VGIAMAFFIRKVLKPDRFRGSHMGEADRILLLILAIVVTFLLWNASRIALGIAEVPDS
                     RPVANLLSGSFGSGGGTDIAERILVWAHLLVILGFLVFLPGSKHLHIATAPANVWLAK
                     DGPTGRLEPNRIDLEAPEEDIRFGVATQKDLSKKQMLDLFSCTECGRCQEACPAWATG
                     KPLSPKLLIMDLRDRVVAEAAGEVELQPLVPNAVIDQVVWDCVTCGACVRECPVDIEH
                     VDTIVDLRRNLVMAESRFPQEASPMLRGVENQENPWGQPASARLDWAKDLDVRVLQEG
                     DPAPEVLFWVGCAGAFDERAQKTTQSVARLLAAAGIDAAVLGPREKCTGDPARRMGHE
                     YLFQMLAEQNVETLNGAGVRKIVASCAHCFNTLANEYPDYGGTYEVVHHTQLLSELIA
                     AGRLQPRTGDGKITYHDACYLGRHNGEFDAPRSVVGGEIEEMPRNRERSFCCGAGGAR
                     MWMEEDAPRINDTRFEEAAATGAETVATACPYCLVMLDDAAKGKASEIRIADAATLLA
                     ESTLD"
     gene            52899..53204
                     /locus_tag="VF195_08300"
     CDS             52899..53204
                     /locus_tag="VF195_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580858.1"
                     /translation="MDVDRIASRYQPDAPYRSHPFRDVSTARGYITEAFSEEELVRCW
                     FGEPVVDGDRAAVEYWAILRSPDGGDVTIAGTFAPPLRRERPGGRASGRLDSAGRRG"
     gene            complement(53252..54589)
                     /locus_tag="VF195_08305"
     CDS             complement(53252..54589)
                     /locus_tag="VF195_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580859.1"
                     /translation="MEVLPLLLREGAGHTLAGHVRELVDRQALSDAEEHRRSMTADAR
                     RGPFDRLRPAAAVAAVHALAVVIWIAFGTELPGGRWLAVHLFTLGVVTNLILALSDHF
                     ARTLTRQPGDAPIAQLIAANVGAVAVFVGVANGRPWLVAAGATVISGVVFVSSWRLRR
                     LRRTSLGARFRWVVRTYERAHGAFLFGALLGALVGTGVLADGWVGAARTGHLHVNLLG
                     WAGLTLLATIVFFGPTIARTRIREGADVRAARALRIGAYGLGIGVLGLVGTGFGGAPG
                     AVARGIAAAGLAAFAWSVTVVSVPVVRASTSSKAPERWSVLAVAAWFVFAAWSDVLVV
                     AAGGWRLLEPVGAAMLLGVLGQAIVVSLGYVAPRLLRLDPATAYTRTEAWAAARALAW
                     NAGVALIVASGVAGSAAAPGFSTAGSVGWVLVLLAAAARALVVGRAALSVRAR"
     gene            complement(54664..55767)
                     /locus_tag="VF195_08310"
     CDS             complement(54664..55767)
                     /locus_tag="VF195_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019355336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX6580860.1"
                     /translation="MRGVAREWVQRRILPEVEDWYEKGEFPARELAKELGELGFFLMQ
                     LDTEWTLGLNSVAYGLVELEIEAGDSGLRSFVSVQSALCGHAIHAFGSEEQKNEWLPK
                     MASGVAIACFGLSEPDSGSDPGSMRTSAKKDGDDWILNGTKMWITNGGCSDIAIVWAQ
                     TDDGVRGFIVPTDSPGFTANDIHRKLSLRASVTSELVMDGVRLPADAMLPEVRGLRGP
                     LSCLNEARYGIVWGVMGAARSCFETALEYSKTREQFDKPIGSFQLTQKKLTDMALELY
                     KGTLLAYHLGRLKDEKGLKPEQVSVGKLNNTREALEIARTSRGILGANGITLEYPIMR
                     HMTNLESVITYEGTEEVHTLVIGEQLTGLKAFT"
BASE COUNT         8679 a        19581 c        19156 g         8512 t
ORIGIN      
        1 ccgcgtcggg cactacgcgg cgatcgcggc gatcgcgttc ggaacggtgt cctccggcgt
       61 caccggcgac cacacctgct ggatccgccc ctcctcgtca accaggaacg aggagcggag
      121 gatgcccatg tacgtgtggc ccccgcggtg cttctcgacc cacgcgcccc aggccgtcgc
      181 cacatcatgg tcctcgtcgg cgagcaacgg gaacccgaga tggtacttct cgtcgaacct
      241 gagctgcttc tcggggtcgt ccggcgagat ccccaccacg tcgaggccga cgcccgcgag
      301 ctcctgccga tggtcgcgga gatcgcacga ctggacggtg caccccggcg tgtccgcctc
      361 cgggtagaag tacacgagga gtctcttgcc gcggtagtcc tcgagccgaa ccgtctcacc
      421 gtgctgctcg agaagcgaga acccgggggc agggtcgccc accttgaggg ccgtcatccc
      481 tgtcactcta tgcgcggacc gagcgatagc gtcatcggag gtccgggttc agcgaataga
      541 ccgggacgat cgtgccctga tcgagtacgt gcccgtcgat cgcggcgcgg aactcctcga
      601 gcatgaaccc ggacccgagg ccgagcgagt cgacgtcgat cgcgtagacg gtcgtctcgt
      661 agtgatggac gtgctcgtcg ttccacggag ggaacggccc gtcccacccc ccgtaggttc
      721 cctcgaggtc gtcgttaccc tcgaagagcc cgcggtaccc gttctgtccc gtgaccgccc
      781 cgagcgcggg cggctcggac ggcttgccgt gagcgacgaa gccgtcgccg ccggcaccct
      841 cggccagctc gtgcacgctg ggagggatgt cggccacgag ccagtgcgcg aagtcgacgc
      901 gcggctcgtc gtcaccgagc gagagaccct cgacgcccat ccgcgttctg tccgcgggca
      961 cgtcgggatc gacgaccgag atcgcgaagg aacgcgtccc ttcgggctcg cccgaccacc
     1021 ggagatgcgg gctcaggtcg cggccggcca tctcggcctt gccgtcggcg gtcggcgtgg
     1081 cgaccgcgtg gatctccgcg atcggctggc cgtccgtgac gctcgtgctc tcgacccgaa
     1141 gcgccatcgc gtgcctcctc cgtgtgtcgt tggggggaag gctcgcaccc gcgcgcgcct
     1201 accgacaacc cacccccggc tcctgagcgt cactcggtgg cgaagagctc ggccaggtac
     1261 gcggcctcgt cgtcgagcgc gcggcgcgtc ttcgtcgaca ggcgctcgaa cggctccgtc
     1321 acgatccgcc cgtgctcgtg acgccagctc cccgccacct gcccttccac gaggtacgtg
     1381 tggatcgagt ggggggtctt cgtgttgaag acgcgcgccc ggtagcgctc ggggaggatc
     1441 tgggttcgtc gcgcatgggt caggagggtg gcgtcccacg cggggaggaa ccggatcggc
     1501 gccggggttc cggggtcggg cagcggtgcc cgtcggacgt cgagcagctc cttacctcgc
     1561 tcgtcgagga agcggcggag cgggatccgc tcgaacgcgg cggtgagcgc gcgcacaggg
     1621 atgcccgtga agctgacgac gtccgcgcgg gaggccggcc cgaaggcggc gaggtaccgg
     1681 cgcaccagat gcgcggtccc ttcctcctgg gtcacggcgg gcggttcgcc gagccagtcg
     1741 gacgcgagcc cgtagagatc ggcgcgcggc tgctcccagg taccgaacgg agggacccgg
     1801 acgaggtcga gccagagccc gacgccgttc cacgtggcgt tgtcgaaccc gagggtcgag
     1861 acgatctcgg cgcggcgctt gggcccctcg gctaggaacc gcgcggtatc ccgggccgcg
     1921 gtggccgcct cgctcgccga tcggcggaag ccccggatcc acccctcccg gcgatgctcc
     1981 cggaccgcgg ccgagaacag ccagaagtcg ctcctcgatg ccatgtggat cgtcgttcgc
     2041 atcatccacg cctggacgac ggccttccgg tgcagcgctc gggtgagttc gtctcgccgg
     2101 aagccttcgt ggcgggttcg cagggcgatg tacccggcgg gggcgtgttg ggtctggagg
     2161 cctccgagcc gctcgatcgt cttcggaacg ctcgtcgtcg cccgctccaa caggtgctgt
     2221 cgagccagga gggctcggtt gagctctcgc gtggtgaggg tccgcgtcgg ttccgggtct
     2281 ccgctcaccg ggaggagcct agcccggcgt cgtgcaagtg cttgtcgctt ccctgagccc
     2341 ctcggtaggg tcctcgacgg acccgtcgga tgtctggcgg ggaacgaccc ccgctgtgat
     2401 cgaagcggcc ggaaaccagg gagaacatcc gatgcagctc gagatcacgg cgaccggcga
     2461 cggctatcgc ctcgaggggg aactcgacat ggcgaccgcg ggtgggttga ccgagctcct
     2521 gctcgctgcc tccgccggcg atacgccgct cgtcctcgac ttctccgggg tgtcgttcat
     2581 ggacagcagt ggcctgcggg ccctcctcga gggagccgag ctcccgaacg ggagcggacc
     2641 cgtcgtgatc caggacccct caccccaggt gcgccgcgtc ctcgagatca cgatcccgga
     2701 cggggtgccg gggctgcaag tccgtccctg aggccttcga tccgttcgtg ctcgcccggc
     2761 tactcgggct ccgatcctgg atccgactcc acgacatggt ccggtgtgtg gtggatcgcg
     2821 ggcacggttc caagccgacc ggcctggtag tcctcgaacg cctggacgag ctcgtcacgc
     2881 gtgttcatca cgaacgggcc gtaccaggca accggctcgc ggatcgggcg gccgccgagc
     2941 accagcacct cgagggccgg cgatcggctc tcctgcgagg aggcggcctc caggcgcacc
     3001 aggtctccgg cgcccagcac ggccagctga ccggtgacga tcggacggcg atccggtccg
     3061 accgtgccct cgcccgacaa cacgtacgcg agggcgttga acgatgggtt ccacgggagc
     3121 tcgagccgtg agccgggggc gatcgtcgtg tggaccatcg tcacaggggt gtaggtctgc
     3181 ccggggccgg tccagccggc gaggtcaccc gcgatcaccc ggacgagcgc accggcgtcc
     3241 gccgacgcca gcaatccgac ctcactgccc cggaggtcct ggtaccgcgg aggggtccac
     3301 ttctcggcct tgggcaggtt gacccagagc tggatcccgt ggaacaggcc tccgctggcg
     3361 accaggtgct ccggtggctt ctcgatgtgc aggatcccgg cgccggccgt catccattgc
     3421 gtgtcgccgt tcgtgatcac gccgccgccg ccgttcgagt cctggtgctc gaagatcccg
     3481 tcgagcatgt aggtgaccgt ctcgaagcca cggtgcgggt gccagggcgt gcccttcggc
     3541 tcgcccggcg cgtactcgac ctcgcccatc tggtccatgt ggacgaaggg atcgaggtcg
     3601 gtgagctcga cgccggcgaa ggcccggcgg accgggaacc cctcgccctc gaaccccccc
     3661 ggggcggtga cgaccgcgcg gaccgggcgg accaccgacg atccacgggc ctcggggagg
     3721 cgcgcgagcg cggtggcgtc gtcgacggtg atcgcgggca tgtcttcccc cgaacctcag
     3781 taccggtgtg cgtattccgg cccggccgcg agcgtgcgcg ccgcgcctcg gcggatcctc
     3841 cgcctgccgg cgccggtcag tcggcgagac cgggcggaac gatccccagc tgcgactcga
     3901 tctcctcggg cgtggccacg gggggacccg gctcgatcag accggcctct cgatcccggc
     3961 gtcgctgcac gcgagccagc cgccgttcct ccgccgccgc cgccttcttc gccttcacgt
     4021 ccttgcgctg tcgcttcccg aaggtctccg gcatggtcat ctcctctgtc tcgttcgtgc
     4081 gccgcccggc gaacgcggcg atgcccgcgt tggagccgtg tgccaggcga tccgtcgatg
     4141 gctgcgcacc gttccgcgcg ccgaccgata tccatcagga tacccgcaag ctgctcgttc
     4201 tcccgcaccc ggggctcggg agagcccccc ggcaactcga aagcgtttcc gtcaccctgc
     4261 gcctgggcaa ggtgttctcg catgctcgga tcgatcggat cacggctccc gcaacgatcc
     4321 gtcgtcgcga tcgacctgct cgtgctcctg tggacggtgg tctggctcgt gctcgggatc
     4381 acggtgggca cgttcgtcga acggctcggc gccgtcggcg aaagcgtcga gggggccggc
     4441 cacgcgatcc atcgggcggg tgacgccgtc ggtcgcctct ccgaggtgcc gctcgtcggc
     4501 gaggggttcg aagccgtcgc gaacgagatc cgcaccatcg gggacgagac ggtccagaac
     4561 ggcagatccg tcgagcagga catcgacacg ctcgcgttgc tgatcggggt cggcctggcc
     4621 gcgggaccga cgcttccggt gctcgcggcg tggatccccc cgcgcgtttc ccgcgaacgg
     4681 gagcgccgcg cgctgcggag atccttgaag agcggcgacg gcgtcgcgct ggcctacctc
     4741 gccaaccgcg ccgtggccgg ccggctgttc cgtgagctcc gcacggcgag cgacgatccc
     4801 gtcgccgacc tggcggcggg ccggtacgag gcgctcgcga ggctcgagct cgaacacctg
     4861 gccctggcgt cgacgtcgtg gctcccccgc tcggcgagca cggctcgcga agagggctca
     4921 cgccccggct gacgagcgat cctcgatctc ccacgcgtgg tcgaggacgt gccatgcgtc
     4981 ccggcgcacg aagtacctgg cggaccaccg cgcgccgccg cggggtccct tctcggggat
     5041 gccgtcgacg agcgcacgtt cgagcccctc gaggacggcg gctcggaccg gttcccgcgc
     5101 cgccgatgca tcgtcctcca cgaagctcgg cggcctcccg gcgatcatgc gcaggtagcc
     5161 cccctcggcg ccgacgacgt gaccgacgat cgctcccacc tcccgcccgc cgccccgcgg
     5221 ccccttgcgg agctcacccc tcaccccggc gacggcccgg tcgaaggacg cccagcacgc
     5281 ccggaggatc gtctcgttcc gcgtgagcca gcgtcgatcg atcgacgtgg cgtcggcctt
     5341 cggcgcgatc gacggggccc cgaagtcggt ggtggcgtcg ccttccatcc gctccacgat
     5401 cgaaagcgtc gaggggcgcg ccggcgtcgt gaagcggacg ccggatccct tcaggatgcg
     5461 cgcgtagcgc gggccgtacc cgaggagcga ctcgagcgcg gcttcctcgt ccttaccgga
     5521 ccggcaccat cccggccagt cgaccgcgcc ggcgaacgcg cgctttccgg tggcctcgac
     5581 gtacacctcg accggcatcc ggcgaggtta cgcgtcctcc agccggccgc cgacgtagct
     5641 cgcgcggccg ttcccgtcgt acaggtactc cacgaccccc tcgagtcccg gctgccccgg
     5701 atccgtgtcg tcatccgtct ccggacgttc cggccagtac acggggtgtc cctcctcgag
     5761 cgtgtcatcg gggatctctt cctcacgccc gtccagcggg ccgtcgacga acgtcacacg
     5821 catcgcctat ccctcctggc agtccgggtg tccacacagg cacggctgag gcccggcgga
     5881 tggtcccgcg gcagcgacgc tccgacaatg gtcggagcag taggcgcccg tctcctcgac
     5941 ctggcagcga cacgcgtcat gcgcacactt ctccatctgc gatcacctcc gggagccccc
     6001 atacccgcct cgggatgggc ggaagcctca ggccttcggc ttcttcggct tcggcttctt
     6061 cttgggctgc ttgggcggga gctcctcggc atgatcgagc gaccgagccg cccactcgcg
     6121 gatacggtcc gggtcgtcgc gccaggcgcc cggcagcacg acgtactcct tcattgtgcg
     6181 gccagccatc ggctcgaaga cgcttccacc cgcaccgagg acttcggcgc gatcggcccc
     6241 cgggagccgc acgaggacat cctcgccgaa gatccccatg aacatgttgc cgttcacgaa
     6301 cgcggagagc tggccgaaca tcggacggat cgacaccgcc gggtgatcgg gcacgacgga
     6361 cgagaagaag tccttcgcct cgtcggtcgg cttcggcagc ttcgaaggag ccacgtgtgc
     6421 aaccctactt ggggagcgtg aacgagatcg tcgtgccggc ggtggtgctc tcggctcgga
     6481 tctcgccgcc gtgtgcctcg atgagtgacc tcgcgatcgc gagtccgagg ccggtgccgg
     6541 gcgagtccgc agcccgcgag aaccggtcga acacgtgcgg gagccgctcc ggcgggatcc
     6601 ccgtgccggt gtcggtgacc gagagaccga caccctctcc cgcggggaac gcctcgagcg
     6661 tgaccgagcc cccggctgcc gtgtgccgga gcgcgttgga caacaggttg ccgagaacct
     6721 cgccgatgcg cagtcggtcg acctcgatct ccggcaggga ctccgtcaca cgggtatcga
     6781 gccggacgcc ggcctcgccg gcccgggcgg cgaacgatcg cacggcgctc tcgatcagat
     6841 ggcgtggctc ggtgggctcg cggtgcagcg tgagcgcgcc ggcctcggcc gtcgagagcg
     6901 tctgcaggtc gttcagcagt cgcgccatga ccttcgtctc gtccaggacg gaccgcaggt
     6961 gggtctcgtc ggccgggtag aggccatcga tgagcgcctc gaggttcgcc tggatcacgg
     7021 caagcggcgt ccgcagctcg tgcgcgacgt cggcgaggag ctgccgacga cgcgcctcgc
     7081 tcgaaccgag ccgctcggtc atcgcattga acgccttgcc gaggcgctgg acgtcgcgcg
     7141 gcccccgttc ccggacgcgg acctcgtagt cgccgtcggc gacgcggtcg gccgcctcca
     7201 tcacctcgcc gaccggcgcc gcgacacgac gtccggcccg ggcgatcagg aagacggcga
     7261 gcgccacgat caggagcccg acgaatccgt agggccgccc gtgtcccgga ccgccggcga
     7321 tcgcaccgcc gacgatcacc gcgaacgtga cgatcgccgc gatcaggatg gccgagccga
     7381 ccacgaactt ccggacgaag cgtcgtcgca tcccgcgcca tgcttcggga ccgtgtggcg
     7441 gccagtcctc gtcctccggc caccagggcg gctgtcctcc gccccagggt ggaccgcccc
     7501 cccacggggg gccatggggg tgctcgtgcc ggtgctggtg cggctcagtc atcggcgaac
     7561 cggtatccca cgccgtggac cgtcaggacg taccggggtg tgcccggctc gggctcgagc
     7621 ttcttccgga tgttcttcac gtgcgcgtcg atcgcgcgct cgtaggactc gatcgccatc
     7681 ccgtggatcg cgtccagcag ctgccctcgc gtgaacacgc gccccggctc gcgggcgagc
     7741 gcggcgagga gctggaactc cgtgggcgtc agttcgacgg gccggccact cgccgtgacc
     7801 cgcatccgct gcggatcgat ctcgatggct gccacgcgga ccggctgctc tcgcaacatc
     7861 tccgcggtgg aggaacggcg gaggacggcg cgcaccctcg ccacgagctc cttcggactg
     7921 aacggcttca cgacgtagtc gtcggcgcct agctcgaggc cgacgatccg gtcggtctcg
     7981 tcgccgcggg ccgtgaggat cacgatcggg acgtgcgagg agccgcggag ctcgcgcgcc
     8041 acgtcgagtc cgtcgatgcc gggaagaccg agatcgagca cgacgaggtc gggtctcgat
     8101 ccacgggcgc tcgcgagggc ggcctcaccg ctcgaggtca cggtcacgtc gaagccggcg
     8161 tcggtgaggt agtcgcgcac gacgcgcgcg atcttcatct cgtcctcgac gacgagcacg
     8221 gtcttcatcc cggcgactct acgccgggtc tatgaaggac ccatgaacga acggcgcgag
     8281 accgtgcgct cacggtccgt gctcgtcagc ggcggcggat cgtcgcaacg accgggaagt
     8341 gatcggacac gtcggtccgc ccgaccgcca cgtcggtggc cgtcaagtgt gacccgatca
     8401 ggatgtgatc gaagatctca ccctcctcgg ggaacgtggc ggcccattct cccgtcaggt
     8461 cctgaacggt gacgaagccg aagtcctgga acttcgccag ctccggagat ccgatctccg
     8521 cgttcaagtc gccggccacg acggtgccgt ccgcgtcgtc gatcgcccgc aagagggcgt
     8581 cgatctgtat cagacgggtt ccatcgtcct cctcgccatc gagatgggtc gcaaccacgc
     8641 ggagcggatc ctgggcgaag gtcacggaga ggtagctccg cgggtgcaga cccccacctt
     8701 gtgggagcga cccccgctcc gtcgcgagga cccggagcgt cggccggtac atgatcgtgt
     8761 tcccgtaggg cccgtccccc gccggcccga atcggtacgg catgcggagc cgccacgaaa
     8821 gccactcgac cacatcggtc atccccgtca cgggccagcc acggcccgcc tcctgcagga
     8881 ccacgatgga tggcctctgt gcctcgatcg tcgacgcgag ggcctcgagg tcgacctgac
     8941 cgtcgttcac ggccgaatga acgttgtagt cgagcagcct gatctcccga ggagcgctcg
     9001 tggatcgggt cgcgggggcg ggcgtcgaca accagagacc caacggcacg ccgacgagga
     9061 gggtggcacc gaaggcagca cggcgaccga ggggagcgag gcggcccgga ggggatctcg
     9121 aacccaccca tgccagcagg aggagcgtga tcgtcgcggc ctcgaggaga tacccgccgg
     9181 gcagcggaag cggccgcggc ttctggtagg cgaccaccag cgccccgagc agagcgggcc
     9241 cgccggcgtg agccagcagc cgggagacgc ggccgcctcc cgcagacgat cggagggcga
     9301 ggacgagcat cgccgcggtg ccagccgtcg cgagtgcgaa gagggtcagc actccggcgc
     9361 cgggtcccag ccggaggccg accataccca ccgccgccat caccgccagc gggatctgcc
     9421 agcgtggcca cgcgccgacc acgaaaatcg tcgcggcgtc gagcgcgagg atgacggcaa
     9481 cagcctggag cgaggtcacg ccggatcgag aagccacgaa cggaacgttc tgcaggaaga
     9541 ccgcgtgcag gaacaggaag gggccgatcg cgaccaaggg gacccacgac gtgctcgatc
     9601 cgtcgcttcg caccgtgcgc gacgtcacga tcgcaagggt catcgctccc aagggaagcg
     9661 cgaccgccac gacccatggc agcaccccct cctgccacac gggatcccaa ccgtggaaga
     9721 cgctccggat cgcgaggtcg acggcgaagc cgagcagaag gccctgtacc ggccggggtg
     9781 ctcgtccggc gaagccgccg gtggccacga tcgacatgag cgcccagaac gcagcggcgg
     9841 tcgtgatcgc cacgagccag aacgggatcg gtcccgcggc ctggagagcg atccggcccg
     9901 ccgccagcag gccgacgccc aggaggacgg agcgcctatc gccgatcacc tcgcggaacg
     9961 acgaaccgag gatcgacccg agcgcgggga taccgaccgt caccgccccc gctgccacat
    10021 accccgcgtc ctcgaagccg tcgaagacga tcgggaagaa cacccgaaga acctggagcg
    10081 agacgacgca tatcaccgcc agaagcgcgg ctcccatcga gggactatgg gggaacagcg
    10141 agagaccgtc acgcggggag caggccccgg ctccgagacc tgctctcccg catacttagt
    10201 tctctcgttg ctcaggcgga ccctgtccgc cccatcctcc tccactctcg tgctggcggc
    10261 ggtgccattc ctcgtatccc tgctcccccg ggccgtgacc gcccgggccg tggtgctcac
    10321 cgggcccgcc gtcatgatcc cagtgccggc gccacatcgc gcccttgaac gcgaacagcg
    10381 ccaggaagaa gagcgggaag aagaggaaga acgggaagaa gccgaaccca tggccgccga
    10441 cgtaccggac gacttcgacc ccctcgccgg ttcgttccag cccttccgtg acgccccagt
    10501 tgtaggcgcc gatcccgacc ccgaccagca gcaggaccac caggatcgcg gcgccccatc
    10561 cgaacccgcg tcgcatctcg aaccttcctt tgccggatgg gtccctccca tccctgagtg
    10621 cagcctcgcg ccgtggtgtg aaggggccgt gaatgaggtg tggagggttc acgtaggctc
    10681 cgttcatgct cgagggcgac cgggtcgtgc tgcgtccgat ccaggaggga gaccgcgagc
    10741 gtctctacga gctggtggag acgatcgagt tgcgcgcgct ctccacccac gatccgccct
    10801 tgccgatctc cctcgaggag atcgaggccc gcgatcggcg atggctcgag gagcgtcaca
    10861 cggactcggc ctggttcgcg atcgatgtcg acgacgagac gatcgggatg tgcgggctgc
    10921 attcgatcga ccactaccag cagtgagcgg acatcgggat caggctcgga aaggactact
    10981 ggggcaaggg gtacgggcag gacgccgtcc gcacgttgtt ggcctacggc ttccgccatc
    11041 tcaacctcgg gaggatctcc ctccgcgtcc tcgcggacga cgagcgagcc gtgggtgcct
    11101 atcgcaaggc cgggttcgtg gaggagggcc ggctccgcgc gcatgcctgg tacgacggcg
    11161 ctcggcacga cgagctcgtg atgagcgtgc tccgggagga gtggacaccg cccgccccct
    11221 agcgaggcga gtcaacccgc gcggcgttct gcgggccgcg gctggctagg tgagcagctc
    11281 gtcgatctcg gcgcgctcgt catcggtgag ccgccacgcc tcgatggctt cggcgttctg
    11341 ccgcacctgc tcggcgctgg tcgccccggc gatcaccgtg gcgatctccg gctggacgct
    11401 cagccaggac atcgcgagct cgaggagcgt gtgcccgtgc gcctccgcga accggctcag
    11461 gagctcgacg acgtcgaagc gctcgtcggt gaggaactcg tcccagtcgc cacggccggc
    11521 catccgggtc ccgggcgacg gtggctcgcc ccgctggtac ttccccgtca gcatccccga
    11581 cgcgagcggg aagtagggca tgaagctcag accgagccgg cgacaggttg agagcacctc
    11641 gtcccgcggc ccgcgttcca ggaggctgta ctcgttctgg acggtgacga acgagcggat
    11701 cccgagctcg ctggctgcct cctccgcctc gtcgagctgc ttcgcggaga agttggagca
    11761 gccgatctcg cggaccttcc cctcgtccac catcctcgac atcgcggtca acgtgtcgcc
    11821 gatcggcgtc gcgtcgtcgg gcgtgtggag caggtagacg tcgatccggt cggtcccgag
    11881 gcggcggagg ctgtcctcgc acgcccgcac cacccagcgt gggtcgccgc ccgtcatccc
    11941 gtccgacggc gtccgcatcc cgaacttcga gacgatcacg gcccggtcgc gccgagagcc
    12001 gagggcccgc ccgaggaact cctccgaatg cccgtcgccg tagaggtcgg cggtgtcgaa
    12061 cagcgtggcg tcggcctcga aggccgcgtc gacgacctcc cgcgtccgct cctcgtcgat
    12121 ccgccgcccg aagttgttgc agccgatgcc cgccagcggc accgggatct ggccgatcgt
    12181 gcgcagctcc atcagcccat gttcccagca gagctagggc tcggcgatct ccctgggatg
    12241 cagcagtccg aacagatagg cgtccgtgag ggcttcccac gacgcctcga tgatgttctc
    12301 gctgaccccg accgtggtcc aagagtgctc gccgttggac gaatcgatca gcacgcggac
    12361 caccgccccc gtcccgtgcg tctcggcgag cacgcgcacg cggtagtcct cgagctcgat
    12421 gtgccggagc tccgggtagt cgttggtgag cgccttgcgg agcgcgtcgt ccagcgcgcc
    12481 gactggcccg tggccctccg cggactcgat gtgccgcgag cccttcgtgc gaaccttcac
    12541 ggtggcctcg gcgagcgggg cctcgccgtc gccgacgcgc tcctccacgt ggacgcggta
    12601 gctgtcgatc tcgaagaacg gctgcgtcca tccggacgcc cgtcgcatca ggagttcgag
    12661 ggatgcgtcc gcgagctcga acgtgtagcc gcgggcctct cgttccttca gctgctcgag
    12721 caccacgggt accgcctcgt cctcgatcgc gaagtcgaac tcggccgcct tcatcctcag
    12781 cgtggtggcg ccgccgaggt ccgacgcgac cacgccgcgc cgattgccca cggcgtccgg
    12841 agagacgtgc tcgtacgcct cgctgaaccg ggccacgccg ctcgcgtgca ggcctgcctt
    12901 gtgcgtgaac gcgaagcggc ccgcgtaggg ctgccgtgaa tcgggcgcga cgttcgcgac
    12961 ctccgcgacg tagtgcgcga cctccgtcaa ccgctcgacc gcgccgggcg gcaggcagtc
    13021 ggcatccatc ttcagcacga ggttcgcggc gatcggcacg atgtcggcgt tgccggtgcg
    13081 ctcgccgtag ccgttcacga cgccctggac ctggaggacg ccggcctcga cgcccgcgag
    13141 ggcggacgcc accgcgcacc cggcgtcgtt gtgcgtgtgg atcccgagcg gcacgccgtc
    13201 cacgcgggcg cggacctccc ccacgatgcg cgcgacgtcc gacggcagcg tgccgccgtt
    13261 cgtgtcgcag agcaccaggc gctcggcgcc ctcctcggcc gccgcctcga gcacgcggat
    13321 cgcgaagtcc gcgttcccca ggtagccgtc gaagaagtgc tcggcatcga agaagacgcg
    13381 gcggccctgg gcccggagat ggcggacggt gtcacggacc atcgccacgc cctcctcgag
    13441 atcggtccgg agggcctttt cgacgtggac gtcccagctc ttgcccacga acgtgaccgt
    13501 ctcggtcccg gcgtcgagga gatcgcgcag gatcgcgctg tcctccgcct cctcgcccgc
    13561 tcgccgggtc atcccgaacg cgacgagctc cgcgtggtcg agcgtctcct tcgtggcgag
    13621 gcggaagaac tccgtgtcgc gcgggttcgc gcccggccaa ccgccctcga tgtagggcag
    13681 gccgagctgg tcgagcttgt ggaggatccg catgcggtcc tccaccgagt aggacagacc
    13741 cggcgcctgt gcgccgtcgc gcagggtcgt gtcgtagagc tcgacgcgag gaccggtcac
    13801 gaggacaccg cctccacgac gaggtcgccg acctcgcggg tcgagtagcc catctcgccc
    13861 gcgcgcatcg acgcgagctt cgtgcaggcg aaccggatcc cggcgtcgat gcggtgggcg
    13921 gctcgttcct caccgagctg ggcgagcagc atgccggtcg cgccgatcgc ggcgagcggg
    13981 ttgatggtcc ccttgccggt ctcggacggc gccgtgccac cgatcggctc gaacatcgac
    14041 acgccgctcg ggttgatgtt cccgccggcg gcgatgccca tccctccttg gatcatcgcg
    14101 ccgaggtccg tgatgatgtc gccgaacatg ttgtcggtga cgatcacatc gaagcgcgat
    14161 ggctgatcca ggaagtagat gcacgcggcg tccacgtgca cgtagtcggt cgtcacgtcc
    14221 ggatactccg gcgcgatctc gttcacgacc cgctggtaca ggtcgcccgc gaacgtcagg
    14281 acgttcgtct tgtggacgag cgtgaggtga cggcgcctgc cgggcagcat cgcgtggtcg
    14341 aacgcgaaac ggacgatccg ctccacgccg agccgcgtgt tcaccgagga ttggacggcc
    14401 acctcgtgac gcgtcccttt ccgatggaac ccgcccacgc cgctgtagag cccctcggag
    14461 ttctcgcgga tcacgaccat gttcacgtcg tcctcggtgt gggacggcac gagcgtctgc
    14521 acgccgggga accggatcac gggacgcagg ttcacgtact ggtcgagctc gaaacggatc
    14581 cgcagcagca ggtcgcgttc gagcacgccg ggggggacgc gcgggtcgcc caccgcaccc
    14641 agcaggatcg cgtcgagacc gcgcatcgtc tcgagttcct cgtcgggcag ggtctccccg
    14701 gttcggaggt agcggtccgc gccgaggtcg aactccacga gctcgatggt gacgtccccg
    14761 cgcacggcgc cgaggacctt gagggcctcg gcgacgacct ccgggccggt cccgtcccca
    14821 cggatcacgc cgatccgata tggccgagag tcggagtgga cccgttcggg ttgaacctcg
    14881 gtcgcgaccg acgcgctcac gacggcactc ctggcaaggg ttgttccaca caccggaacg
    14941 cagttccacc agacggaaca ccggtatcgg cggagccgac tgggagcgcc cacctcctca
    15001 cggggtgacc tccgtgttcg gttcgctcat ggcggtctct cccccggacg ccgcgatcga
    15061 cgcgtcggcc accatcgccg cgcgattgac cgcggcgagg taggccctcg cgctcgcctc
    15121 caccacatcc gtggcgaccc cgcggcccgt gaccctccgc ccgtcgatct ccagctggac
    15181 gacgacatct cccaaggcgt ccgaaccacc cgtgaccgac gacacgttga acgtgatcag
    15241 cttcgcctcc acgcccgccg cgcgctggat cgcgccgcag gcggcgtcga tcatcccgtc
    15301 gcccatcgcg gattcctcga tcacctcgtc accgcggcgg agcctcacgg tcgccgtcgg
    15361 cgcgaggtgc gtgccgccgc ccgaggccag ggactcgagg acgagaagat cctccttccc
    15421 gctgccgacc tcctcggaca cgatcgcggc gagatcctgg tccgtgatct ggatcttgcg
    15481 gtcggcgagt tccttgaagc gcgcgaacgc gcgatgcatg tggtcgtcgt cgatcgagat
    15541 gcccaccttg tcgagcgcgt cggcgaacgc gtggcgcccc gagtgcttcc ccaggacgat
    15601 ctggttgccc tcgagcccga tctcgaccgg gtccatgatc tcgtaggtcg cccgattcgc
    15661 gagaaccccg tgctggtgga tcccgctctc gtgcgcgaac gcgctcgccc ccaccaccgc
    15721 cttgttcggc tgcaccgagt acccggtgag cgcggagacg agccgcgagg tccgcacgat
    15781 ctccttcagg ttcagcccgt gccgcacgcc gagcgcctcc cggcgtgtcc gcaggatcat
    15841 cgccatctcc tcgagcgagc agttcccggc acgctcgccg atcccgttca cggcgacctc
    15901 cacctggcgc gcaccgttct ccacgccggc gagcgagttc gcgaccgcga gcccgaggtc
    15961 gttgtggcag tgggtggaga tgatcacgtg ctccggggtt cgatcccgca cgatccgcac
    16021 gagctcgccg aactccgcgg gcgtggcgta gccgaccgta tcggggacgt tgatggttgt
    16081 cgccccggcg ttcaccgcga cgtcgaagca ctcgagcagg aagtccagct cggtgcgggt
    16141 cgcgtcctgg gcgctgaact ccacgtcctc cgtgtagccg gccgcgagct tcacgccctc
    16201 ttcgatcccg tcgagcacct ggcgatggga catccggagc atgtcctcca tgtggatctg
    16261 cgacgtcgag atgaacacgt ggatccggaa ccggtcggct cccttcagcg cctccgccgc
    16321 gcgctcgatg tcgcccggca ccacgcgagc gagcgacgcg atcaccggac cccgcacgct
    16381 cgtcgcgatc tcgcggacgg cctcgaactc tccgtcggac gagatcgcga acccggcctc
    16441 gaggatgtcg acctgcagcc gggcgagctg ctccgcgatc tcgaccttct cgtccttggt
    16501 cagcgcgatg ccgggagcct gctctccgtc gcgcagggtg gtgtcgaaga accggactac
    16561 gtcggggtcg ctcacgacgg cggcaccttg tcctcgctcc gcagccacgg catcatcgtc
    16621 cgcagctgcc ggccgacgtc ctcgagctgg gacgctcgcg cctcgcgccg cttggccagg
    16681 aactccggga acccgccgtc ggcctcggcg atccatcgct tcgcgaagga accgtcctgg
    16741 atctccgcga ggagctgccg catcgtctcg cgcgtgccgt cgtcgatcac ccgcgcgccg
    16801 gagacgtagt caccccactc ggcggtgtcc gagatggagt ggcgcatcca ggagaggccg
    16861 ccctcgtaga tcaggtcgac gatgagcttc acctcgtgga ggcactcgaa gtaggcgatc
    16921 tccggctggt agccggccgc cacgagcgtg tcgaagccgg agcggatcag ctcggacagc
    16981 ccaccgcaca gcaccgtctg ctcgccgaac aggtcggtct cggtctcttc cttgaacgtc
    17041 gtctcgatga tgcccgcgcg ggcggcgccg atcccgtggc cgtacgcgag cgcgcgctgg
    17101 cgagctttcc cggtggcgtc atggtggatc gcgacgagcg cgggcgtccc gatgccctct
    17161 tcgtaggtgc ggcgcacgag atggccgggt cctttcggcg cgatcatcgt gacgtcgacg
    17221 tcgggcggcg gggtgaccgt cccgaagtgg atcgagaagc cgtgcgcgaa catcagcatg
    17281 tcgccggcgt tcaggtgggg tgcgatctcc tccgcgtaca ccttcccgtg cgaggtgtcg
    17341 gggaggagga ccatcacgac gtcggcatcg gcgaccgcct ccgcggtatc gaccgcccgg
    17401 aggccggcct cctccgcctt ggttcggctc gacgagccgg gtcgcaggcc cacgcgcacg
    17461 tcgtaaccgg agtccttcag gttctgcgcg tgcgcgtgac cctgcgaccc gaacccgagg
    17521 atcgcgatct tctgccccgc cagcgcggat acgtcggtgt cctgctcgtg gtagatcgtc
    17581 gccatcgttg gtcttctccc gtcccttggc ctagacgccc ccgccggtcc actccggcga
    17641 gtcctccgcg cccacggcct tcgcgacccg cagaccgctc ttctccctga tcccgcggtc
    17701 gccgcgcgcg agcgcgatcc gcccggtccg cgagagctca acgatcccga actcggacag
    17761 cagctcccgg agcgcctcga gcttctcggg cttgccggag gcctcgaccg tgagcgtcgt
    17821 cttcgtcaca tcgacgatgc tcacgcggaa cacctcggcg atctccatga ccctcgctcg
    17881 cgcgtcaccc ttcgcccgaa ccttggcgag catcagctcc cgctccaccg ccgagcccgg
    17941 ctcgtgctcg aggatcttga tcacgttgat cagcttgttc agctgcttcg tcacctgctc
    18001 cagggacttc gactcgacgt tcacgacgat cgtcatccgg gagaggccct ctgcctccgt
    18061 gggtcccacg gcgagcgagt cgatgttgta gccacgcgcc gagaacaggc cagcgacgcg
    18121 cgtcaggacg ccgggcttgt cctcgacgag aacggagatc gtgtgcaggc tcatgcgatc
    18181 ggcgcctccg ccgggtcgtc ctgcggctgc aggccctccg gccccgtgat gatgtcgtcg
    18241 ttcgaggcac cggccgggac catcgggaag cacatctcgt tcgcgtcgca acggaagtcg
    18301 atcaccacgc ttcggtcgtc gaccgacagc gccttctcga tcaccgcgtc gacctcctcg
    18361 ggccgctcgg ctcgcagacc gacggctccg tacgcctcgg cgagcttcac gtagtcgggc
    18421 acgtcgtagc ccaggaacac ctgggagtag cgctcctcgt agaagagctc ctgccactgc
    18481 cgcaccatgc ccaggttggc gttgttgatg atcgcggtga cgaacgggat cttctccgtc
    18541 gtcgaggtgg cgagctcctg cgaggtcatc tggaagcagc cgtcgccgtc gatcgcgatc
    18601 acgcgccggt cgggctgtcc ggccgccgcg ccgagcgccg ccggtaccgc gaagcccatc
    18661 gtgccgagcc cacccgagtt gatccacgtg cgcggcttct cgaaggacca gaactgcgag
    18721 gcccacatct gatgctggcc cacgccggcc acgacgatcg tgtcgccgtc gctgtgcgcg
    18781 cggagacgct cgatcgcgta ctgcggcttg agcggtccgt ccggctcctg gtcgtagcgg
    18841 agcggctgct cggccttcca cgtggcgagc tgctcgagcc acgggctcct gtccgggatg
    18901 ccggcctcgt cctgtcggcg cgtgagctcc gcgacgagct tcgccgtcac gtccctgcaa
    18961 tcccccacga tcgggacatc ggctctgcgg ttcttgccga tctccgctgg atcgatgtcg
    19021 atgtgcacga tcttcgcgtc gggcgcgaac gtggcgagct tgccggtcac acggtcgtcg
    19081 aagcgagccc cgaccgcgac gagcaggtcg gccttctgca tcgcggtcac cgccgtgtag
    19141 acgccgtgca tccccggcat cccgaggcac agctcgtggg cgtccgggaa cgcgccgcgc
    19201 gccatcaggg tcgtgacgac cggcaggtgt cccaccgtcg cgagctcgta cagctcctcg
    19261 gtggcctccg ccttgatcac gccgccgccg acgtagagga ccggtcgctt cgcctcgagg
    19321 atcagcttcg tggcctccac cacctggcgc atgttgccct tggtggtggg ccggtagccc
    19381 ggcagatcga cctgatcagg ccagctccag gtcgtctcgt tcaccagcac gtccttcggg
    19441 aggtcgacga ggaccgggcc gggccggccg gtgcgcgcga tgtggaacgc ctcgcggatc
    19501 gtctcggcca cctcgccggg atcggtgacg aggaagttgt gcttggtgac cggcatcgtg
    19561 atgccggtga tgtccgcctc ctggaaggcg tcgttgccga cggccggcat ggggacctgt
    19621 ccggtgatcg cgacgatcgg gaccgagtcc atcttcgcgt ccgcgagcgc ggtcacgagg
    19681 ttgcagccgc ccgggcccga cgtggcgatc gcgacgccga cctttccggt ggcccaggcg
    19741 tagccctccg cggcgtgccc ggctccttgc tcgtgccggc acagcacgtg gcggacgccg
    19801 gactcgatca gcgggtcgta ggcgggcagg atcgcgccgc ccgggatccc gaagacgatg
    19861 tcgacgcctt cgccctcgag ggccttgaac agcgcctggg cgccgctgac cttcatcccg
    19921 cgaccgcctg ccgctcctcg acggggccgg tctcgacccg cttcacggtg gcacccaggc
    19981 ggcgggccag gttgcgacag gcacgctcga actcccacgg ggtccagtcg cgacgcctgc
    20041 tctgcagctc ctccccgccg aagccgctca ggtaggtgac ctcggaggac aggtagccct
    20101 ccagggtcgt cctcgtgctc ggatcatcgg tctcgacgct cacctggccc ggtccctccc
    20161 acagcacgct ggcgacggtg acgccgtcct tctcgaactc gtagcgcatc ggatgctcct
    20221 ttcgtcttct cggtcagctc tgttctcgtg ttcggctggt cgtgggcaga aaaaaacccc
    20281 cgcgtggcga gggtgtcggc gcgggacgcg ggctctcagg cccggccgcg cctagcggat
    20341 aagtacgagg acggtgctcg gcgtggtcct cgcgctcttc atggttgaat cagtgtcggc
    20401 gctcgcccga ggagtgtcaa cccccggacg gccgatggcc tctgcgccgg ggggaccaga
    20461 taccatcacc ccatggagcc gctccccgac gaggagtcgc ccgtcccccg agtccatccc
    20521 ctcgagatcc cgttcgtgtg gttcgggctc cagctggtcg agtacgcggt cgtctggctc
    20581 ctgatcacga ccttcctccc ggaaacgatg cccgggccgg tagggtggac gctcctcctc
    20641 gtgctcgtga tcggcctcac gatcctcaac tacaggatcc ggcgccgatt catccctcgg
    20701 tagtcgcccg caccgtcagc cggcggaact cgacgcggcg cggacccgct ggagatcggt
    20761 ctgctcgagg atccgctcga cgatctcgct cgggagatcc tgtttctcct tctcccgctt
    20821 ctcggcctcc ttggcctgct ccacctccac gcgagcggcc tgccacgcgg accagtggcc
    20881 ggagaccagg gcccagagcg agagggctgc gacgtaggtc acgctgctga tccagccggt
    20941 gaggatcgat gtcgggatca tcaggatcca gaagatcgtc agccagccgt tcaaacggcg
    21001 catgaacacc ggatcacctt ggacgctcgc ccacaaggac ttgaccacgc tcatgggcgg
    21061 acacataccc ttctccatga actcccaagc gtctgagcgg cgacgtaacc cccgctcacg
    21121 cgagatcctg atcagcggcg aagtgatctc cgacgacgaa cccatccgcg ctgatctcga
    21181 attcacggat ctgcgcgtcg tgggcgctcg ccctcgtctt catcacggtg atcccgcgct
    21241 tcacccggga ctccccccgg aggaactgca gcagcacgac gttgtcggag aggtgagaaa
    21301 taccgaattc ggataagcgc gagacaccga agaggtcgcc tacctcctgc gtcatcatca
    21361 cgctcacccc ttgacgggag caccgctgca ggagggagta catgtactca cggaaacgga
    21421 tctgatcggt ggacgcagcc tgcagatcgc cgagactgtc gatcatgatg cgacgcgctc
    21481 ccgtcgcttc caccgtgccc aggagctcat gcacccattc gtccaagtac agatcgacag
    21541 gcgtgcgata catgagcccg acgccctctt cgtccagaga ccagccgaat ccgttggcga
    21601 cccgctcgag ttgggcggcg ttctcctgca gcgtcgcgat cacaccaggt tcgccgcccc
    21661 gagccccctc gaagatgaaa tgcaggccca tcaaggtctt gcccgatccg gacggaccgg
    21721 ccacgagagt cgaggccccg ggccagtacc cacgcccgag catctcgtcg aggatcttga
    21781 tacccgacga gatcttgacg tcttccagct cgtacggtgc ttgatcgacc gggtcggcca
    21841 atcgaggaaa gaactgaacc ccgtcgccgg agagccggta ggcatgcttg ccagaaagga
    21901 agtcgctccc acggagcttg agcacctgga agacacggag ctcgcggccc cctactcgct
    21961 cgacggagag cgacaggatc gagtcggcga ccgcgaactc ggcggccccg gcgatctcgg
    22021 cctggtcgta ctcgccgacc cagaacgacg tcacagggaa cgctgatagc cgaccggcga
    22081 gctcgtggag gaatcgtcgg aactcctcgt ccctgcgtgc gtacggatgc agcgccttga
    22141 agctgtcgat gatgatcatc ccggggcgtc gctccttgag gaacgcgctg atcctctcga
    22201 gaaccccctg gaggccttcg tcgttcacgg tcccgcccag atcctcgtag aacaccgacc
    22261 tgccgatcgc cccgcgatcg aagaagctca gcgtctgacc gaatcgcagg atcttctcga
    22321 ggggctcgga gaccgtggag aggtagatcg ccggccgctc ccgcgtcgcg ttgtggaacg
    22381 cgtactgctg agcgaggatc gtcttccccg atccagggag tccgatgatg aggttgatcc
    22441 cattgtcggg cagacctccg cccaacatgt cattcaaccg ttccgagccg ctggacaccc
    22501 ggtcgctcaa ggcgagagca cctcggttcg gaggaacgcg ctctggccga gcaggtcctt
    22561 gacggtcgac accagttgca ggggatcgag gggtttctgg aggaacgcgt cggcgccata
    22621 ttcgagcgcc tcgtcgcgcg attccagcgg cgatatcgcg aggacgagcg ctccccgttc
    22681 ccggaaggtt cggcagagat ccggccctgc accgcctgag atcaagaggt cgaccaccgc
    22741 gagatgaggc gactgctcct cgaagatggc ctcggcctcg cgcgcctcga gcgccatgat
    22801 cgtctcgtag ccctcggtcc tgaggaagta ctcggcgagc tcagccgcat gcgggtcgcg
    22861 ttcagccaac atgatgacca accgggtctc ggaccccgtg atcgcgacct gcgcatcggg
    22921 atcgtcaccc agacgatccg tgaggagccg gtgtgcatcg gctggctgca tgccctgctc
    22981 gatgctcctg aggacgaagc gcagctgctc gacgtggtcc cgcgtgtaga ggcggtggcc
    23041 gccagggctc cgatcgggca taacgacgcc gtaccgctct tcccaggtgc gcaaggtggc
    23101 ggtggggaca ccgagcatcc tggcgaccgc cccgatgctg tagatggcag ctgtcacagc
    23161 acgggagcct agttgaatgt gaatgagttt gccagtgcga atctcctcag aacaccttca
    23221 acgtttctgc aggtggccgg gccgagcatc tgaacggtct ccatcagcat caccttcgag
    23281 cttgacctgc gcggccatga ggaggtagct tcttcaaccc tgaggcaggg tcaggacgct
    23341 gatgcaggaa agccgggaac gcgagcacga ctcagctcga accacgtctg agcgactcgt
    23401 caaggaaaac cgcagatacg accctggaac gggcggcggg ggatcgaaca acaacggtgc
    23461 ctccgctgag gcgaggtacc gagctctctt cgagagcgcg ggtgacgcga tcctcatcgt
    23521 ggaccgagaa ggcctctgca tcgatgcgaa cccgagagcg accgagctcc tcgggtacga
    23581 gcgacgggag ctgctctcga tgcccgtcct cgatctggtt gcggccgaac cgggatgggc
    23641 ggatgaagag ttccgacacc tcatcgatca ggagcgttgg acgggagagc tggaactgcg
    23701 gcgcgaggat gggtccctcg tcccggtgga cgtgtccgcc acgagggtgg acatcggatc
    23761 cggtccggcc atctatctgg cagcgatgcg cgacctcagc gatcggaagc agctgatcga
    23821 agagcgcgcc gagctcgtca gcgcggaggc ggaggcgcaa acacaagcgg aagccgcgag
    23881 gcaacgcgtc cgcttcgtct ccgacgtcag tgagctcctc gccgtttcgc tcgactaccc
    23941 ggcgaccctc gttcgcctcg ccgaggtggt ggtgcgggag atcgctgatc tctgcgtcat
    24001 cgacctcgtc caagagtcgg gcaccttcga gcgggtcgcg gtggtgcacg ccagctcggc
    24061 gaagcaagcc ttagccgagc agctcggtgg ctacgcgcct cggccgggga gctcggatcc
    24121 tgtcgcccga gtcttcgcga ccggggagcc gcaggtgttc tcggatcagt cacctggatc
    24181 cttgcgggcg atcacccagg atgaggggca cctgcggatc gctcgggaac tcggtgtcca
    24241 gtccttcctc tccgttccgc tgatcgctcg gggtcgcacg ctcggggtca tcagcctggt
    24301 gtccacgaac ccagacctcc ggtacggcga gtcggacgtg cgactggccg aggagatcgc
    24361 ccgacgtgcc gccgtcagga tcgacaatgc gcggctctac gacgaacggg atcacacggc
    24421 gaaggtgttg cagcggagcc tgctgccccc cagccttccc tccatcccgc atgtggagct
    24481 cgcagcgcgc tacctgccgg tgggcgaggg gaacgaggtc ggcggcgact tctacgacgt
    24541 cttcgactgc ggcgacggga actgggcggc ggcggtgggt gacgtctgcg gcaagggacc
    24601 ggaggcggcc gctgtcatgg gactcgctcg acacacattg cgtgcggtgg cccgcttcga
    24661 acgcaaaccg agcgccttgc tcagagagct gaaccgagcg ctcctcgagc agagcgtcga
    24721 ggagcggttc tgcaccgtct gtcatctgcg cttgcggccg agccgagatc atctccgcat
    24781 cacgctgtgc tctgcgggcc accctctccc cttgctcttg cgacgaaacg ggaccttgtc
    24841 atcggtgggg gaatcggggc agctcctcgg ggtgttcccc gaggtctcgc tggtggactc
    24901 cgtcgtcgac atggatccgg gcgacacgtt gctgatctac acggacggtg ccaccgaaca
    24961 gcgccgcgag gctgtcgcgg gggaggagcg tctgcgcgac gcgttcgctg cctcgctcgg
    25021 tcgagacgcc gaagcgacca cggcgggagt ggaggaggct ctccgtgccg ctcgcggatc
    25081 gtcaccgcag cgcgacgacg tggccctgtt ggtcatccgg gctgcggagt ccgatccgcg
    25141 tccgtcggag tcatagcagc ctccggttcg accctcggac agcaccggtg ctagcgttcg
    25201 gccgccatgg tcacggacgt ctccgatccc gcgctcgacg ccgtgatcca cgcgatcccg
    25261 tcgtgggaag gcaaggagct cgagatcatc ccgatcgccg agggccgcac caatcggaac
    25321 ttccgcgtca cagccggcga cgacgccttc ttccttcgac tctcagagaa gtccaccgaa
    25381 ctgctcggca tcgaccggac cgccgagcag gattccgtcc gggcggcggc ccaggccggc
    25441 gtgggacccg aggtgttcgt gtatctcccg gagctcggct gcctcgtcac ccgctggatc
    25501 gacgccgagg cgctctccga gggtgacctc gagcggcagg agatcctcgc tcccgtcgtc
    25561 gacgcgatcc aggcgatcca tgcggccccg cagctctcgt ggtcgttcaa cccgttccgg
    25621 atcgtggagg actaccggcg catctccgag gaacgcggcg tgatcgtgcc cgacgcgttc
    25681 gacggcgcgc acgagaaggc gtcccggatc gaagccgcct tcacgagcga cccgatgccg
    25741 ctggtcccct gccacaacga cctgctcgag gcgaacttcc tcctgcgcga cggccacgtg
    25801 tgggtcgtgg accacgagta cgcggggatg ggcgacccgt tcttcgacct cgggaacctg
    25861 tcgatcaaca acgcgctctc cgacgatgcg caggagcggc tgctcaccct ctacttcggg
    25921 gagccgaacg acgcgcaccg agctcgcctg aagctgatgc ggatcatgtc cgacttccgc
    25981 gaggcgatgt ggggcgtcgt gcaacagggc ctgtccacgc tcgacatcga ctacgtggag
    26041 tacgcgacga agcacttcga ccggctctcc acgacgatgg cggaccctcg cttcgacgac
    26101 tggctcgaga ccgcgcgagg gatctgagcg gtggcggacg atcgcgcgcg cgtcgtcgtg
    26161 atcggcggcg gcgtggcggg gacgagcatc gcctaccacc tgacccgcct cggctggacc
    26221 gacgtgctgc tggtggatcg cggggagctc acgagcggct ccacgttcca ctcggccggc
    26281 ctcgtcgccc agctcaggaa ctcggtgagc cacacgcgga tgatgatgta cggcgcggag
    26341 ctgtatccga ccctcgccga ggagaccggc gtcgatcccg ggtggcacca ggtgggcacg
    26401 ctgcacctcg cgtcctcgcc gtaccggatg gaggcgctcg cccgtcaggc gggttgggcg
    26461 aaatcgttcg gcctgcccgt cgagatcgtg tcgaccgagg aggccgtcga gcggttccca
    26521 ctgatcgacg cgtcccgcgt cctcggcgcc cagttcgtcc cgaccgacgg gcacatcgat
    26581 cccaccgggc tcacgctctc gttcgcggcc ggcgcgaagg ccgcgggcgg gcgcgtgcgg
    26641 acgggcgagc gcgtggagga gatcacggtg cgggacggcc gcgttgcggg cgtggtgacc
    26701 gaccacggcg cgatcgaagc cgacgtcgtc gtgaacgccg gagggatctg ggcacacgag
    26761 ctcgggcggc tggcaggcgt ggagatcccc gtggtcccga tggagcacca gtacctgatc
    26821 acgcggccga tcgagggcgt gcgcgccgac ttccccacgc tccgggaccc cgacaacctc
    26881 gtctacgtcc gggaggaggt gggcggcctc gtggtcggcg gttacgagcg gaacccggac
    26941 ccgtggcacg tcagcgatcc gatcccgcgc gacttcaacc accggctcct gccggagaag
    27001 tgggagcggt tcgagtcgat cgccgacggc gccttccggc tgatcccgcc gctgcagacg
    27061 atcgagatca accggttcat caacggcccc gaggcgttca cgcccgacga cgacttcatc
    27121 ctgggcgaga ccgaggtggc gggattcttc gtggcggccg gcttctgcgc gcacggtgtg
    27181 acgggcgcgg cgggcatcgg acggtggacg gccgagtgga tcgtcgaggg tgagccctcg
    27241 attgacctgt cgaagatgga tgtccggcgc ttcgggcgcc actacgcgag ccgtgactac
    27301 gcgctcgcga aggcctacga gatctacgcg aagcactacg acgtccggta tccgggcgag
    27361 gagaacgagg cgggacggcc gctccgcatg gcgccgacgt actcgcgcct ggagaagctg
    27421 gacgcggagt tcggcgagaa ggccggttgg gagcgggcga actggttccg gtcgaacgaa
    27481 gacccggcgc acgaggaacg gcggccgcgc gggtgggccg gagagcagtg gtcgaccgcg
    27541 atcgtcgccg agcacctggc cgtccgcgca agggcggcgc tgttcgacga gacgagcttc
    27601 gcgaagttcg aggtctcggg tcccggggcc ctcgacctgc tgcagcgcct gtgcgcgaac
    27661 gacgtggacg tgcgcgtcgg ccgcatcgtg tacacgcaga tgctgaaccg gcgaggtggg
    27721 atcgagtcgg atctcaccgt cacgcggctc gccgacgatc ggttccgact cgtcaccggg
    27781 accgcctccg ggagccgcga cctcgcctgg atccgccgac atcaggaagg tcacgacgcc
    27841 catgtccgtg acgtcacgtc gggcctcgcc tgtctcggga tctggggtcc ggcggcgcgc
    27901 gagatcctcg cgtcggtctg cgacgacgac ctgtcgaacg agggcttccg atacctcacc
    27961 gctcgcgaca tcgaggtcgc ggacgtgccc tgcttcgcgg cgcgcgtgac ttacgtcggc
    28021 gagcttggct gggagctgta cccatcgacc gagttcgcgg tgcggctgtg ggatcgtctg
    28081 atcgaggccg gagcgccgca cggcctcttg cccgccggct atcgggcgat cgactcgctc
    28141 cggctcgaga aggggtaccg gtcgtggggc gccgacatca cccccgacga ctccccgctc
    28201 gagggggggc tgggcttcgc ggtcgcgttc gacaaggatg cggacttcct ggggcgggat
    28261 gcggtgcgac ggctccgcga cgaaggcgtc acgcggcgcc tccgatgcct gacgctcgcg
    28321 gaccctcgct cgtccaccct cgggaacgag cccgtgcgcg ccggcgacga gatcgtcgcg
    28381 cgggtcacgt cgggcgggct cggctacacc gtcgggaaga gcatcgcgtt cgcgtacctt
    28441 cccgacgggc tcgacgggct cggcacgaag gtctcgatcg aggtgttcgg ggagtgggtc
    28501 gacgccgagg tcgtcgacga cacgctctgg gacccttccg gcgagaggat cagggcgtga
    28561 tggagcaacc gatcttcgag ctcgacgggc aggtggcgct cgtgacgggc gccgggcgcg
    28621 gcatcggtcg cgacctcgtc gtggcgctga cgggggccgg cgccagggtg gcggccggca
    28681 tccgctcgcc cggcgatggg gggtcggtgg tggacgaggc gcgcgtcggc ggcggcgacg
    28741 ccgaagcggt cacgctcgac gtcaccgacc gcggctcgat cgagcgagcc gtggaagaga
    28801 ccgtcgagcg gttcggtcgc atcgacatcc tcgtgaacaa cgcgggcctc gggacgaacc
    28861 acgacgccct cgacgccacg gaggaggaat gggacgagct gttcgccgtg aacgtccgcg
    28921 ggctgttctt cgcgagccag tcggccggac gccgcatggt cgagcagcgc cacggccgca
    28981 tcatcaacat ggcttcgcaa gccggcctcg tggggatccc gcggcacgct gcctacagcg
    29041 ccagcaaggg cgcggtgatc gcgctgacga aagtgctcgc gctcgaatgg gcgccgttcg
    29101 gagtcacggt gaacagcgtg gcgccgacgt tcatccggac gcccggcacg gccgagcgcc
    29161 tcgatagccc ggagttcctc gccgacgtca tggagcggct gcccgtcggg cgcgtcggga
    29221 ccacgacgga cgtcgccggc gcggtgatct acctcgcgtc gccggcagcg agcctcgtca
    29281 ccggaaccgt gctggtcgtc gacggcggct ggacggcgca gtaaagcgtt acgcgcccgg
    29341 tgcggggatg tcgtcgccgg cgaatatcga gccgttccag tacgcctcga gccgatgggt
    29401 cccggcgcgc caccccttgc ggtcgaggtg gagctccgca ggcggctcca tcctcccgaa
    29461 gtcgtagtgg agcgtgatgc cttccgcggt catcgcttcg aaccgatgcg gccgccggga
    29521 ggaccgtgtg aagctcgtgc ggcggctcga tcggggcggc tccgcgtggg cctccaggta
    29581 gatgtgcgag ccgaccagcc ctcggtgggg gtcgagccac acccacgcgg cgccacctcg
    29641 ctccgcgatg taggcggcgg cgcgttcgga gacgaccagc ttcatcgccc gatgatggca
    29701 cgacgggcgg ggccgccgtc ccggcggccc cgccccaccg tcaggcttcc gcgggggcga
    29761 tccctgccag atcctcttcc tctggcggcc tcacggggac gaacggcttg ttgcgctgca
    29821 ccgcgaagta gtagatcgcc ccgatgatgc tgacgagggc gaagaccgtc cagatcacgg
    29881 ggatctcgtt caagaacccg atcttgaaat cgaccagctg ctctccctcg acgaagtagt
    29941 cggtctggat cggcttcggg ttcgagaaga tccggaggta attcgcgtcc gaggcgttgg
    30001 ggtcgaacgc ggaggttgcc gaggatggcg tcaggaagtt caggagcatc gcggcgcccc
    30061 agaggatcgc gacgacgttc acgaccttgc cccacgagcc cagggagaac ggcgcccgcg
    30121 tcttcggcca tcccctggcc cgcgcgcgca tgaacgcgag gttgccgagc aggtagctga
    30181 agtagatcga tgccgtcgca ccgacggcga tcgtgctcgc accggcgaat tggatgaacg
    30241 ggatcgccga gagcgctccc acgatgatgc acgtggccac cggtgtgtgc agccttgggt
    30301 tgaccttcgc catcgacttc gagaagggca gttggtcgtc cctcgccatc ccgaacgcga
    30361 gccggatcgt cgacgtgagg atcgccatgc agcacacgaa gatggacgcc acaacgacga
    30421 gcagatagat gacggtgagg gcatcggaca ggacggcgtc gatgatcgtg gtcgggccga
    30481 agaacccggc cacggcctcc cccatgtccg gtacggcgat caggacaccc cacaggaaga
    30541 ccgcgccgat cacgaacgct ccgatcacgg acgccagcac cgccttcggt gcctcctgcc
    30601 gcgggttgtg cgtctcctcg gagagggtcg acgccgtgtc gaagccgtag atcacgtaga
    30661 ggctcatgaa catcccgacc aggaagaagc cgagagtgac gtcggcgggg aactggaaga
    30721 tgttccccgt cttggcggag tcgacgatca ccccagcgcc cgaattgttc tgcgtgacgg
    30781 ccaggaagat cgcgaagacg accatcccga gcacctcgaa gaacaccccg gtgttgttca
    30841 cgatcgcgac gagcttcacg ccgtagatgt tcaggatcgt gatcagcgcc agcgtcacga
    30901 gcgccgcgat ggcctggtcg acgtgtccac cggtgctgaa gtccgccgtg aggttccaac
    30961 cgaacatgat gttcagcatc gggaagagcg cgagcggcag ggtcgcgcac acggaggcga
    31021 cggtgaggat cccggcgatc agatagaacc aacccgtgaa ccaggcgtac cctcggttgg
    31081 ctaagtactt ggtccactgg tacaccgagc cggcgaccgg gaagtggctc gagagctccg
    31141 cgaagttcaa cgccacgacg aactgcatcg ccgcgaccac gggccacgtc cagaacagga
    31201 agccaccgag ggcagccatc ccgaggaaga acagcgtgaa gatgccggtc gagggcgaga
    31261 tgtagctgaa cgccaccgcg aacgagctga acgtgtggag ctctcgcttc aactcctgct
    31321 tgtacccgaa ccgttcgagg tccgccgtat cgcgggcgac ctgatcgccg tgcaccgaca
    31381 tccgctcccc ctccctgcgc cacgctcggt ccgcccgtgc ttgctgcggg ccgccgcgag
    31441 tgtgaacccg acggcgcgag gacgcaaatc ggttgggagg gagcctcagc gggcgagcgc
    31501 cgcgaggaac tcgtcgaggt ccggcgcgac gtggaagctc ggctccacgg aaccctcgaa
    31561 cgcctcgacg gtcttgaaac cgttcccggt gacgaaggcc acgacctcct cgtcgggccc
    31621 gatcacgccc tgctcgacca ggcgcttgag gttcgcgatc gtgacgcccc cggcggtctc
    31681 ggtgaacacc ccttcggtcg aggccagcag gcggatcccc tccaggatct cggcttccgt
    31741 gcaccactcg atcgcgccgc cggtctcccg ggcgacctga agcgcgaagt agccgtccgc
    31801 gggtgagccg atcgccaggg agcgagcgat cgtgtccggt ttgaccggcg tgacctcctc
    31861 ggcgccgtcc tggaaggcct gagcgaccgg ccggcaaccc tcgggctggg caccgctcac
    31921 ccggtaggcc ttctcctcca ccgccccgat cgccatgagc tcctggaacg cacgatggac
    31981 cttcgtgagc agcgcgccgc tcgcgatcgg tacgaggacg tgatcgggta cgcgccagcc
    32041 gagctgctcg gccacctcga atccgagggt cttggagccc tcggcgtagt aggggcgcat
    32101 gttcacgttc acgaacgccc acgggagcgt gtccgcgacc tcggcgcaga gacggttcac
    32161 gtcgtcgtag ttgcccttca ccgcgacgac cttggccccg tacgtctcgg tggcgaggat
    32221 cttcgcgcgc tccaggtcgt ccgggacgaa cacgtagcac ggcatcccgg catgtgcggc
    32281 gtgcgcggcg gccgcgttcg cgaggttgcc cgtggaggcg caggagagcg cctcgaaagc
    32341 gaacgcgcgc gccatcgtgg cggcgaccga gacgacccgg tccttgaacg agtacgaggg
    32401 gttcacggag tcgtccttga tccagagatc ccggaggccg agctcggccg ccagccgatc
    32461 ggcgcggcgg agcggcgtga agccggcgcc gaggtccacc cggtcgagac ccggaccgcc
    32521 gggcaggagc agctcgtagc gccagaggct ggcgggcccg gcctcgacct gtttccggaa
    32581 gtccacgccg tcgatcgcgt ccaggtcgta ggaaggctcg agcggaccga agcactcctc
    32641 gcagatcgtg agcggctcca cgttgtaccg ggcaccgcac atccggcact gcagggccgc
    32701 cgggctcgtg atcgccgccg tcacgtcgcg atcaccatcg cgcgattcac ccggtgatgg
    32761 ctccgtgctc ggcgccgccg acgagcttcg cgtacttggc gagcgcgccc ttcgtgtacc
    32821 gaggctcggg ggccacccag gcagcatgcc gggtggcgag cgtgtcgtcg tccacgagca
    32881 gctcgaggga ccgcgcgtcc gcgtcgatcc ggatccgatc gccgttctgc acgagtgcga
    32941 tcggtccgcc gatgaacgct tcgggcgcga tgtgcccgac gctgtagccc ttcgtcgcgc
    33001 cggagaaccg gccgtcggtg atcagcgcca cgtccttgcc gagaccggcc cccgccaccg
    33061 ccgcggtgac agcgagcatc tccggcatcc ccggtgagcc cttcggcccc tcgtagcgga
    33121 ccacgatcac gtcgcccgcc acgatgtcgc cggacgtgac cgccgcgaag gcgtcctgct
    33181 cggagtcgaa cggacgggcg acgccttcga agacgaggcc ctcggcaccc gcggacttca
    33241 ccacggcgcc gttcggggcg agattgcccc ggaggatcgc gatgccgccc cacgggtgga
    33301 tcgcgttcgt gcccggatgg acgacgtcgc cgtcgggcgc gggcggagcg acctcggcga
    33361 ggttctcggc ctgtgttctg ccggtcacgg tgaggcagtc cccgtggagc agcccgaggt
    33421 cgagcaactc tttcatcacg acggggactc cgcccacgcg atcgaggtcg ctcatcacga
    33481 acttccccgc gggccggacg tcgaccaggt ggggtgtggc ccgggagatc cggtcgaagt
    33541 cgtcgagctg cagatccacc tccgcctcgc gcgcgatcgc gaggaggtgg agcacggcgt
    33601 tcgtcgagcc cgcgagcgcc atcacgaccg tgatcgcgtt ctcgaacgct tccttcgtga
    33661 ggatgtcgcg cggcagcggc cctcctgcgg cgacgagctc cgcggcgcgg atcccgctct
    33721 cgcgcgcgag cacctcgcgc cggtagtcca cggcgggcgg gcccgccgcg cccggcagcg
    33781 agagcccgac cgcctcgccg accgccgcca tcgtgttcgc ggtgtacatc cccgcgcacg
    33841 acccggtgcc ggggcaggcg gcgcgctcga tccccagcag ctccgactcg tcgatcgttc
    33901 ccttcgcgtg cgcgccgaca gcctcgaaca cgtcctggat cgtgatctcc cgcccgccgt
    33961 aggtaccggg gaggatcgtc ccgccgtaca ggtagaccga cggcaggttc agccgggcca
    34021 tcaccatcat catcgcgggc tcggacttgt cgcagccggc gatccccacg agcgcgtcga
    34081 atcgctccgc gtgcatcacg agctcgacgg agtccgcgat cagatcgcgg ctcatcagcg
    34141 acgccttcat cccctcgtgg cccatcgcga tcgcgtcgga gacggcgatc gtcccgaact
    34201 cgacgggaac cgaccctccc tggcgcacgc cctccttcgc gagcgcggcg agcttgccga
    34261 ggtggtagtt gcacggggtc acctcgttcc acgacgacgc cacggcgacc tgcggcttgc
    34321 cgaagtcgtc ctcggagaac ccgacggcgc ggagcatcgc gcgggccggc gcgcggtgcg
    34381 ccccctcggt gacctcccag ctccggggac ggtcgtgctc gcgttccatg tgcgtaccct
    34441 ccgtgaggaa ctgcgaagat gcgaagcttg agcctagcaa ggaggatgag ccgcccgtga
    34501 cgaccgttcc ccccgacgcc ggccgcagct ggtggttgcg agaggcattg gccgacgacc
    34561 ccggcgaacc cgcctcgccg ttgcgaggag acctcacgac cgacgtcgtg atcctgggcg
    34621 gcggctacac ggggatgtgg accgcgctcc acctgaagga gctcgatccc gggatcgacg
    34681 tcgcgatcgt cgagcaggac atcgtcggcg gcggcgcgag cggccgcaac ggcggcttcg
    34741 tgaactcgtt ctggggcgac ctcgactacc tcgcgcacca cttcggtgac cgagccacga
    34801 tcgcgctctg cgaggcgggt gaggagagcg tgaaggcgat cggccgcttc gccagcgatc
    34861 aggggatcga tgcctggtac cgcgcggatg gggagtacgt ggtggccgcc tccgatgagc
    34921 agttcggccg atgggcggac ggcctgatca ccgccgaccg cctcggcgtg agccagcact
    34981 tccaggtcct gtcggccgac gagctgcgcg agcgggtcgc ctcgcccgtg ttccgcggcg
    35041 ccctcttccg gacggtcggc gccaccgtgc atcccggtcg cctcgcgcgc gggatgcggc
    35101 gtgtgctcct cgagcgtggg gtgcggatct tcgaggactc ccccgtcacc cggttcggtg
    35161 ccggcgaccc ggcgcgagcc gagacgccgg gtggcacggt gcaggcgggc gtcgcggtgg
    35221 tggcgctgaa cgcctgggcc caccactgga aacggttccg caggaccgtg gtcgtccgcg
    35281 gctcctacat cgtgatgacg gaaccggcgc ccgatcgact caaggagatc gggtggacgg
    35341 acggctccgg cgtcgcggac atgcgctccg cggtccacta cgtgcgcacg acgcccgacg
    35401 ggaggatctc cttcggcctg ggcggcgtgc agccgaacct cgcccggcgg atcggtccga
    35461 ggttcgcgta cgacccccgc gcgatcgaga tcgcgatcgc ggatctccac cggatgttcc
    35521 ccgggttcgt ggacgtgccg atcgaggcgg cgtggggtgg gcccatcgac gtctccggcc
    35581 accacctgcc gacgttcggg acgctcggca aggggaacgt ccactacggg cacggctaca
    35641 ccggcaacgg cgtgggaccg gcgcacctcg gcggtcgcat catggcgaac cggatcctcg
    35701 ggaaggcgga cccggtcctc gacctgccgc tcgtggatct cgagcccttg cggttcccgc
    35761 cggagcccgt ccgctcgccc ggcgccctgg tcgcgaacac ggcgatccgc cggaaggacg
    35821 aagccgagga cgacggcgag gagccgaacc cactgatcga cttcgtcgcg aggctccccc
    35881 gccgcctcgg ctacaacctc ggcccctagg cgccgaacgg ttcgtagccg ggtgcgacga
    35941 gcggccacgg gttcgtccgc tcccatgctt cggcggcagt gagtgccatg ccgtcacgga
    36001 accgtggcgc cgtgatctcg aggccgaacg gcacgccgtc cggggtgacc cccgccggga
    36061 cgctgatcgc cggatggccg gtcatgttct gcggatcggt gttgaacgcg tccggtgaat
    36121 caccgacctc acccgtctcc gggttcgtcc catcggcccg gatccacgcc acgcagttcg
    36181 tcggcgtgag cagcacggcg tcgtcaccga ggaggagatc gagctcgcgg acgtacgcga
    36241 agcgcctgcg tcgcgcggcc atgtagtcct cgaaggatgc cctccgtgcg aactccacga
    36301 cgccgcggaa gacctgggag aagcgctcga ggttctcctc gcagaagtcc atgccgaagt
    36361 ggtggacgtg ttccatcgcg cagatgagca gccagtcttc gctcacgtgg ccggcccgga
    36421 agatccgatc cgcgtcgatc cgctccaccg ggacaccgac gtcacgctcg aacgaggcga
    36481 cggcaacgtc gaacagctcc tgcacggact cgggcaacgg gccgtagtcc acgagtcgcg
    36541 gcgacgcgaa ggcgcgagtg ggcatctccg cgaccatctc cagcggcatc ggcagcgcgg
    36601 tcgggtcgcc ggggaccgga ccggcctcga tcccgaggag caggcgcacg tctgtgacgg
    36661 tgtgtccaag cgggccgaac gtggacagat cgatccactc ggggatcggt tgccggccga
    36721 cgacgccgtt cgtgggcttc agcccgacga gacccgacca gccggccggg atccggatcg
    36781 acccgccgcc gtcggtcgcc gtggcgatcg gggcgatgcc tgcggacatc gcggcgcccg
    36841 acccgccgga ggacccaccc ggggacgcct cggggttcca ggggttcctc gtcgggccgt
    36901 agaggaggtt gtcggtgaac ccggcgaacg cgaactcggg ctggttcgtc ttgcccacga
    36961 cgatcgcgcc ggcggccttg aggcgccgag ggatcagacc gtcggcggcg gccgccggcg
    37021 cgtcggcgaa caccacggag ccgtaggtgc tcggcacccc ttcgacgtcc tccatgtcct
    37081 tgaccagtag ggggatcccg gcgagagggc cgacctcctc cccggcggtg acgcgcgcat
    37141 cgagcgcgcg cgcgtcgtcg accgcctgtt cccggaccga gatcaccgcg ttcagcggcg
    37201 ggttcaggcg ctcgatacgc tcaagcgagg tacgcacgag ctcctcggcg gacagctgcc
    37261 catctcgcac ggccagtgag agctcctcga gcatcggccg agcgtagtcg acaccacccg
    37321 atggcggcgg cacgagcccc ggcgccagga ggtcaggtgc ccgaagccag ctcccgccgc
    37381 agggtctctg cgaaaagcgc ctcgctccgc gcgcggaggt ccaggtagag aaggaccgag
    37441 acgagcgacg cgaacggaac cgtcaggatc tgtgcgatgg cggtgaagat ccatcgcacg
    37501 gcccagttgt cgccgccgat ccccccgatg accccggaga tgatcccgac gatcaacgct
    37561 gcgaccacga tcacgccgag cgcatgccag aagtgtccct tcaccaggtt ccacgatcgt
    37621 cccatcgcct cggtgccgcg ccggccctcg acgatcagca ccgggatcga caccgacagg
    37681 agcacgagga agatgatccc ggggatcacc agcagcacga aacccacggc gacgatcaga
    37741 cccaccagaa gcgacacgag gatcacgctc cacaaccgct tgagcccata tcggtagctc
    37801 gcctcgacat cgaccgggtc accgatcgtg gcctgcgcgg cggcgcgcag gatggcggcc
    37861 tgcaggatgg ccgagatgag caccgcgatc aatgcgccga tcgccccaac gaggagcaag
    37921 acaccgaggc cgcgcccggc gtaatcgaac gcaaccacgc ctcccgtgtt ggtcacccgg
    37981 atccgctcgg gggcgaacac cactccggag aacagagcgg cgatcagcga cagcgggacg
    38041 accacgaccg cgacgaccag gatcagctgc gacgcgttcg ccttatagat gttgaaggct
    38101 gcagacagga tgtcgcccac cgtcttcggc gggatgccgc cctgcggtcc gcccatcatc
    38161 ggcggtggtg gagggggagt cggcggcgtt ggcccgccag ggacatcgct catgcgtggt
    38221 cacctttccg ctcgtgtagg tgaagcctag cctgtgagtg ggctgtgagt gcccatcacg
    38281 tccacgcgct ccagcacgga ggagcactct tgtcacgggc catccaccct tggcacgctg
    38341 agatctcatg agtctctcgt tctcgacgtc aggctcgatc gccgggatgc tggcgcggtt
    38401 gcccagttct gggaggcggt caccggacat cccaaggagc aggtgcatca gccgggcaac
    38461 gaccactggg tggtgagccc gcccgatggg accttgccgc ggctggtctt cgtcaccgtg
    38521 ccccaggaga agtcgatcaa ggatcggatg cacctggacc tgctggcgaa ccactcgatc
    38581 caggaagacg aggtcgccct cggttgaccc tcgttggacg cgccgcctcc tacactccgc
    38641 cggatggatc ttcgactcag cgccgccgac agggacctcc agcagaaggc gcgtgacttc
    38701 accgaccagg tcctcgtccc gctcgaggac gagtgcgagg ccaacgacgg actcacgccg
    38761 gagagccacg cggccgcgaa gcaggccgtc ctcgactggg acttccacgc gatcaaccac
    38821 gccgaggccg acggcggccg cgggcacgac ctcttcgccc agatgctgat cgaggagcaa
    38881 tggggtcgcg cgaccggcgc cctctgggac atcccgtggc ggccctcgat ccccctcgcg
    38941 ggaggcaccg aggagcagaa ggagcgattc ctccgacccg cgtgtcgcgg cgagcgccgg
    39001 gacgcctacg cgatcaccga ggagggcgcc ggctccgacc cgacgatggt cgagacgacg
    39061 gcggggcgcg acggcgacga ctgggtgctc gagggcgaga agtggcatgt gacctcggga
    39121 gacgtggcgg acttcttcct cctccacacg cacgtggacg gggatcccgc gaaggcgacg
    39181 atcttcctcg tggacaagga cgcgcccggt gtgcggctgg tccgcacacc caagtacatg
    39241 cacacgttcg tgttcgagca cccgatcttc gcgttcgaga gcgtgcgcgt aggcggcgac
    39301 cggatcctcg gccagatcgg ccgagggttc gagctcacga aggactggtt cgtcgaggag
    39361 cgcctgatga tcggcgcgcg gacggtcggc gccgccacgc gcgccctcga gctctccctg
    39421 gacttcgcga aggaccgccg ccagttcggc cggccgatcg tcgacttcca agccgtggag
    39481 ttcatgctcg ccgacatggc cgcggagctg atggccgcga agtcgatgct ctaccgggtg
    39541 tgctggcacg cgggacgggg cggcgccgat cgcaaggagc tgcacgcgat ggcggccgcc
    39601 gtgaagctcg tctgcagcga gacggccggc cgagtggcgg acaaggccgt gcagatccac
    39661 ggtggacgtg ggtacatgcg cgaacatccc gtggagcggc tctggcgcga gctccgcgtc
    39721 gaccggatct gggaggggac gtcggagatc cagcgcgtgg tgatcggcaa cgagctgcgc
    39781 aaacggggtg ccggtcgcta cacgggctgg cccacggagt gatctacagg atgttcgggc
    39841 ggagcggttc cgacatgacg ttgagccagt ccagcaacgg gtaggacagc cccaccagac
    39901 cgaggacgag ccccatgaag tagctgccgc caccctcccc gctgctcgac gtcatgatcg
    39961 aggagaggac ggccatcaga ccgaggatgg cggccagggc gacgagctga tcggccagct
    40021 ggccgccggc cgcgacctcc gcggagtact gcccgaggat cttgctgtcc ggctggaccc
    40081 ggtagatcca gaggacggca ctcgcgaggg cggccgccag gccggtgacg accgagatcg
    40141 cccccaaagg gttcgtatgc ctgcccgaac ttctgaagac ccccgccatc aggcggtatc
    40201 caagcaccct cggacttcgg tgggcaagcc ggggatgtcg aggccctcgc cacccttcac
    40261 gaagtcgtac cgcgtgcccg accactcacc cggcagtcct gggtacggcg aaggctccgt
    40321 gtccgcccac tggaatccgc tccaatcggg cgagcggaac ccttcggtgt cgtagggcgc
    40381 cacggcggcg acaccgtcgc cgaactcccg cgactcgacg aggaatcgtc cctggaaccg
    40441 aaggtcgtcc tcggtgtaca gcttcacgta ccagcgctcg acgaccgaga gatatccctt
    40501 gtcagagggc ggcgactcgg gaccggtgaa gtaccgcatc tccacgacgg tcggctcgcc
    40561 gggaagctgc acgctccggc ggatgatgcg taggggcttc tcgaacagcg ccgaatccgc
    40621 gccgggcgag acccagccct ccgcacaggt ttccgacgcg tcgccgctcg tcggtagcgg
    40681 gtccgggggg agcgccgggg gtgtgggaga cgcgagcgtc ggcgtcgacg ggaacggcga
    40741 tgccgacgtc ggcaagggag ggggcgggaa cggctccggt tcgccctcgc acgcaaccag
    40801 gaagagcagc agcgcgactg cgatcatgcg tttcgtcatg agatgacccc tcggggaccg
    40861 accgaggata gcgggcttga tagaaagggg ggcgatggac accggaccct acgggcacac
    40921 gcgcgacgag cgccacccga tgccgctcag ccgtcgacgc ttcctacgga cgagcgccat
    40981 ggccgtcgcc ggcggggtcc tcttctcgtg caccggcggc accaggatcc cgaaggtcga
    41041 cgacacgatc ccggagacgg acacccgatg gccgatcaag cgcgtcatgt acgtgatgct
    41101 cgagaaccgg agcttcaaca acctgttcgg gaagttcccc ggcgtccgag gggggaccac
    41161 gatcggggtc gagaacggcg tggagaagcc gctgatccgc tgcccggagt ggctgcccgg
    41221 cgacatcccc cacgatctcg ccgcgcaccg ggcctgcgtg aacaacggga agatggacgg
    41281 gttcggcacg gggatatatg gctcgacgta cggctacacg atcttcgacg aggagcagat
    41341 ccccaactac tggcactggg cgcgtgagta cagcctctcg gacacgttct tcgcgtccgc
    41401 agcgggcccc agctacccga accacttcta cttcgtcgcg gggtcctcgg gcggcgtcac
    41461 cgacaacccc gagaacatcc ggacgaggcc actcgaggag ggaagactct tcaagagctg
    41521 gggctgcgac gcggtggggg acgacgtgtt cgtcctggtg acggacgagg ccgggaacgt
    41581 gacgaagcac aacacgtgct tcgagttccc cacggtcggc gagcagctca cccgggccgg
    41641 cgtcgactgg gcctactact ccgcggtccc gggacagccc gggtacttct ggaacgccta
    41701 caacggcatc tccaacctga tccacaaccc cgatctctgg caccagcacg tccgatcggt
    41761 cgacacgctc ctcgacgaca tcgacgcgag cgccctgccg cccgtcacct gggtgacccc
    41821 gcggttccag ctgtccgacc atccgccgtt ctccacgggc cacgcgcaca actgggtcac
    41881 ggagatcgtg aacaaggtca tgcgctccga catgtgggag cacacggcga tcttcctgac
    41941 gtgggacgag tggggtggct tctacgatcc cgtcgttccg ccctcggtcg atcacctcgg
    42001 cctgggcatc cgcgtcccgc tgctcacgat ctcgccgtac acgcgccggg gcgtgatcga
    42061 cgatgagcga ggcgagttct cgacaccgct gcggttcatc tcggacaact gggggttgga
    42121 gccgctcacc gaccggatcc gcgataccca caacctcgag cacgtcttcg acttcgccgg
    42181 cggaccacgg gcgccggagc tcgggaccga gcgggcgccg acgttctcgc cctcgccgtg
    42241 ggtgttcccg cgcgatacct acaccggctg ggaaccgggg acggagccgg tggaggagcc
    42301 cctgtagccg ctagacagcc cagcggcgat ccgcgtaccg ccagacccaa cccttcttgg
    42361 cgcgaacgac atcgggatcg cggggatcga gggggaggat catctcgagc tcgcgagggc
    42421 ggcgaggctt cggcttctcg atccgttcgc gcttcattgc cgcccacctc ccgtgctccg
    42481 ccggatccgt cgcgagtggt tcgcgtccgg tggcctggcg gatcgcgtct caggcatacc
    42541 ccctcgatga gagttgtatc ccaggcttcc cgagccgggc catgaccggc atcacagctc
    42601 gggtgtgacc ttcgcccgag acgggtcagg ccgggatcac gagcgcgtcg acggcgacgc
    42661 agcccgaggg cgacacgatc accggattga cgtcgagctc cgcgatctgg tcaccgaggt
    42721 cgatcgcgag gagggacagc cgagagacgg cgtccgcgag ggcgtcgacg tcgcaccgcg
    42781 gcgcgccgcg aacgccgtcc aggagggggc ggatcgccag gccatcgatc agacggcgtg
    42841 cggcgtccac accgatcggg gggagcgcga gcttgcggtc gcgcaggacc tccaccaaga
    42901 cgccccccgc cgcggcgagc acgagcgggc cgaacgtcgg gtcgcgcacg atgccgagcg
    42961 ccacctccac acccggcggc gccatcgccg ccaccgtgac ctccgggccg agccggtccg
    43021 ccaggtcccg gtaggtctcg cggagcccgc cgacgtccat gatgcccacg cgaacgcccc
    43081 ggacctcgga cttgtgcgag atccctggca ccgccgtctt gatcgcgacg ggatagttga
    43141 tccggtccgc cgcctcctcc gcctcgtcga gcgaccgggc cgggagcgcg tcgaccacgg
    43201 ggatgtcgta cgcggccagc aggcccagcg ccgcaacctc tgagaagtcg ccggcctcgg
    43261 ccagccgccc gcgccactcg gcttcgaccc ccggtgagga cgtgggcgcg ggatccacgg
    43321 ccagccgact ccgccccgtg gcatcggcga gcaagtgctt cagcgcccgg aggccggtca
    43381 cggtgccctc gagcacgggg atccccgctt cgcggaggat cccgacctcg tcgtcggcga
    43441 tcgtcgccgg caggttcgag atcacgcaga acggcttcgt cgtccgctcc cacatgtcgc
    43501 gcgcgacctg gaggtacccg acgtcgtacg gctcgccctg tcgagtgagg tccaccacga
    43561 acgcgacggc cgcggtctcc gggtcgtcgt ggagcgcctg gaagctctcg acgtagatcc
    43621 ggtcggcgtc gatcccggtc ccccaggcgt cgagcgggtt ctcggccacg agcccgggat
    43681 ccagcacgtc ggcgatcttc gtcttcgtgt cctccgagat cgcggcgagc ggcacacccg
    43741 tgtccgtggc gaggtcgacg aagagcactc gctccccgcc ggaatcgtgg agcgacgcga
    43801 tcccggtccc gtccgtcacg cggcgcggac tggagaagag ctcgagcgcg tcggccatct
    43861 cctcgaggtc gcggcactcg tgcacgccgt acgcttcgaa gacagcctca taggcgccgt
    43921 gctcacccgc cagcgcgcca gaatgcgcca ccaccatctg cttcgagccc tcggtgcggc
    43981 cgaccttgag cgcgaacacc ggaacgccgc gttccgcggc tttcgcgagg gcggcgacga
    44041 acccgtccgg ctgacggacc gtctcgagga gcagggccac gacctcggtg gagtcgaggt
    44101 ccagcgcgta cgagaggtat tcgtccatcg tggtgacgat ctcctggccg ctcgacacca
    44161 gcaggttgaa gcggatctgc cggtcgttga acaggaacgc cgcccagccg gacccggagt
    44221 gcgagatgaa cgtcacgccc cccgagcgca ggtcgtcggg ggtcaggaac ccggttgccc
    44281 gcaggttcgc gtcggcgtgc acgaacccca tcccgttccc gccgcagagg gccatcccgt
    44341 gctcccgggc gatggccgcg acccgctctc cgaggggagc catcccgggc tccggcggct
    44401 cctcctcgta gagcgaagag aacgtcacca cgctgccggc gcccgcatgc ccggcttcac
    44461 gcacggcctg ctcgatccgc tggttcgcga cgccgatgat cgcgaggtcg acgggctcgg
    44521 ggatctcgcc gatcgacggg acgcatggat gcccgagcac ctcgtcgtag ccggggttca
    44581 ccgggtagat cgcgccgtcg tacccgccac ggtgcagctc gagcagcatc tgcagaccga
    44641 gcgacccctc cttcacggag gcgccgacga gcgcgaccga gcgcgcctcc agcatccgct
    44701 ggagcggcgt ccgttcgtct tcggtcacga ccgcttcttg agtgccctgt ccttcacggc
    44761 cgcacggtac gccttccacc cggggatacc gttgaccccg ccgccggcgt gggtggccga
    44821 ggagccgtgg tacaggccct tgatcggcgt gcggaagtcc gcgtaccccg gcgccgggcg
    44881 catgtggaac agctgatcca ccgacagctc gccgtggaag atgttgcccc cgatcaaccc
    44941 gagctcctgc tccatgtcgt acggaccgat cacctggtag tcgatgatcg cgtccttgaa
    45001 gttcggcgcg agctcgtcgt agaggtcgaa gatccgcttc gcgtacgcgt cgagctcctc
    45061 gcgatgcggc tcgttgaccc agtcgtcggg cacccactgg ctgaacatcg agaacacgtg
    45121 cgttccttcg ggggcgagct tcttgtccat cgtcgtcggg atcacgccgt cgacgaacgg
    45181 ggccacggac gctttgcggt ccatgtgcga gtcctggaac gcgcgctccg cgtatcgcgg
    45241 tgagaagcac agctccacgg agcccgtgtg gtgctcctgc agctcctttc cgggcatcgc
    45301 cgtgaagtcc ggcagctccg acatcgcgac gttgatcttc acgacgccgg agcgggtctt
    45361 ccagcgctgg atgtccgtga cgaagtcggc cggcagatcc gtgggcggga tcatgtcgag
    45421 gaatccgatc ttggggtgca cgcacgtcac gccgatcggg gcgcggagct cggtcccgtc
    45481 ctcgagcacg accccgtgga accgacctcc ggacgtgagc atcgacttca cgcgtgcgtt
    45541 cgtgcggacc tcggcgccga acgcctccgc ggagcgccgg atcgaatcgg agacggcgcc
    45601 cataccgccc tgctggtagc cccaggagcc gaggtgaccg tcgccgacgt cgccgatcga
    45661 gtgatggagc atcacgtacg ccgtgccggg ctcgtccggc cccgcccacg tcccgatgat
    45721 gccgttcacc gcgagcatgc ccttgatcgc gtcggactcg aaccagtcgt ccagcaggtc
    45781 cgtgatgctc atcgtgaaca gccgtgtgac gtcgccgacg ccacggaccc cgagcttgcg
    45841 caccttccac gcggtgcgcg ccgtctcgag caggtcgccg atagcgaggg atccggcgcg
    45901 gggcgggctc tgcaggagca gcggtcccag gatgtcggct acgcccttca gccacgcctc
    45961 ccactcgggg agcgcgtccg cgtctttctt cgagaactgc gcgatcgagt cgcgcgagcg
    46021 ctcggcctcg tcggcgtaga ccatgatcgc gcggccgtcg gggaacgcct ggaagtaggg
    46081 gccgaacggc gtgacgtcgt agccgtgctt cttgagctgg agctcgttga tgatcgtcgg
    46141 cggcatcagc gacatcacgt aggagtaggt ggtgacgttg atctccgggt gatccgcgaa
    46201 cggagcgctc gtgtcggcgg cgccgcccgt cttgttcctc gcctccagca ccacggtctt
    46261 cgcgccgtgc cgggcgaagt aggcccctgc cgtcaatccg ttgtggcccc cgccgatcac
    46321 gatcgcgtcg taggtgttcg tagccatccg ccttcctttc tctggccgcc gtcgtgaccg
    46381 agggccggct ccgcgaatga tgccctattc ctgaacggtg agtcaaacgc cctcgtggga
    46441 gccccgcctt tgatttccgc tgctcaacgg ggtacggttc gcacggccac cggggtgccg
    46501 gtgggctacg acccgcatga tctctgcaca ccgttcaggg agttcgatgg cgaaggaacg
    46561 agcgaaagcg aagccgggcg tcaaggtcgg gaaagaccgc cgacaggaga tcctcgccgc
    46621 cgccgcgcag gtcatcacgg atcgcggcct cgccgagacg aggatccagg acatcgccga
    46681 gacgtgcggc gtgtccaccg gcctgatcct ctactacttc gagtcgaagg atcgcctcct
    46741 ggtcgaggcg ctcacgtacg cgaacgacca gttctacctc cggctctcgc gcgagctccg
    46801 caagaacccg tccgcgcggg cgcagctcga ccggctgatc gagctctccg tcccgggtct
    46861 gctccccgag ttctctttcc tcgacgagtg ggcgctctgg gtggagatct gggtccgagc
    46921 gctccgcgat ccggagatgg cgaaggagca cgaggtgttg aaccgccggt ggcggcgctc
    46981 gatcgcggac atcgtgcggc acggccgctc gaccggcgaa ttcccggagg gactcgacgc
    47041 agacgagctc gggatccaga tcggcgccgt ggtcgacggg ctcgcgatcc aggtgctgat
    47101 ggaccacggc gacgtgacgc ccgagaacat gctgcgcgtg agccgtgagg tctcggcgaa
    47161 gctgatcggc ttcgccctcg agccagccgg cgcgtagcgt catcagcctg ttcgcccctg
    47221 agtaccctgt gcgcgcgatg gcgcctcgcc ccacgatcct cgacgtcgac accggagtgg
    47281 atgacgccct cgccttgctg tacgcggtgg cttcaccgga ggtcgagctg atcggagcca
    47341 ccagcgtgat ggggaacatc acggtggagc aggcgacgga gaacacactc gcggtgttgg
    47401 aggcggcggg tcgcggcgac gtcgaggtcg cgcagggggc ggctcggccg ctcggccgcg
    47461 accacgtccc gttcccggtc gtccacggcg aacgagggct cggccgagcc gccccgccac
    47521 caccggccgc cgagccctcg acccgctcgg cggtcgagat gatcgtcggc tcggcccggg
    47581 agcgacccgg cgaggtgctg ctcatcgcca cgggcccgct cacgaacgtc gccttcgcgc
    47641 tccgggagga gcctcacctc cccgatctgc tcggaggctt cgcgttgatg ggtggggcct
    47701 tcgcgcgggg cggcaacgtc accccggccg ccgaggcgaa catctggatg gatccgctcg
    47761 ctgcggacga ggtgttcacg gcgttctccg gcgccgacga cgcgcggctc ccgatctgcg
    47821 tcggcctcga cgtcacggaa cgggcggtga tgcgccggcc ggacctcgat gcggtctgcg
    47881 cgccggcgcc cgactcgccc gtcgggcagc tgatcgaagg cgcgacgagc ttctatatgg
    47941 acttctacgc agcggtcgtg ggcgaggacg gctgccgcct acacgacccg ctcgcggtag
    48001 ccatcgcgat cgacgagtcg ctcgcgcagc tcatgacgac gcgcgtcgag gtcgagacgg
    48061 aaggtcgttg gacgatgggc gagacggtgg cggacctggc aggggtgcgt gggagtccct
    48121 ggcccgttgg ctgggagccc gaggcgaacg cgcgcgtggc actcgaggtc gcggcggagg
    48181 gattcatgga acggttcgtc gaacgcgtgc gctcgctcgt cgaggcgagg gcatgacggc
    48241 ttcggtcgtc gtgatcggcg cgatcaacgt cgatctcgtc atctccgggg ctcccttgcc
    48301 ggcgcccggc cagacggtca ccggcggcgt gttcgcgcag catcacggcg gtaagggcgg
    48361 gaaccaggcc gtggcggcgt cgcgggcgct cggtggccaa ggtgtcgtgc tcgtgggggc
    48421 cgtggggaac gacgcgctcg ggcatgacgc gctggaggcg ctgcgagctg agggcgtggc
    48481 gacgtcggtc gcgatgcgac ccggcgttcc cacgggcgtg gcgctgatct gcgtgggtcc
    48541 cgacggcgag aaccagatct cggtggcgcc gggggcgaac gcggggctca ccgccgccga
    48601 tgtgacctcg gccctcggac aggtgcacga gatcggggtc gtcctcgcga gcctcgaggt
    48661 ctcccaggcg gcggtacgat cggcggccgc gtggtgcagc gagcacgaga cgcccctggt
    48721 cgtgaatccc gcgccggtgc agccctgggt cgcagacatg gcgttgcatg ccgcctacgt
    48781 caccccgaac gagcacgagc tcgaggcgct ggggtcgctt cccgacggtg tggtggtggt
    48841 ggagacacga gggcccgagg gtgtgctcat ccggcggggc ggcgaggaga cgcgggtgga
    48901 ggcgccgaag gtcgaaacgg tggacacgac cggcgcggga gactgcttca acggcgtctt
    48961 cgcggcgggc ctggccgagg ggatggacgt cacggatgcc gccgagcgag gcgccgcggc
    49021 cgcttcgctc tcggtcacga tggtcggcgc acgcgaaggg atgccgactc acgccgagct
    49081 cgacgcgttc ctgcgcacca cgtgatgccg accctcacgc ggagcgggta cggtgctggc
    49141 gtgagcgagg aacggcggcc ggtggcgtga acgtcgtcgt cctgacgaag tgggttcccg
    49201 agcccgaggg cacgccgagc ctcggcgacg acgggctcct cgtccgcgaa ggcgccgacg
    49261 gcgctctcga tcccggtgac gagtacggcc tcgaggccgc cctgcaactg gtggaagctg
    49321 cgggcgacgg cgagatcacc ctcctcacga tgggtcccga gatagcgcag gcggccctcc
    49381 agcggggcct cgcgatgggc gcccacaagg gcatgcggat caccgatgac tccctccgcg
    49441 gcgccgattc gctggtcacg gctcgtgtgc tcgcagccgc gatagagcgc cagcccttcg
    49501 acctcgtgat cggcggcgtc gagtcgaccg acggctacac ggggatcgtg cctgccgcga
    49561 tcgcggagct cctcggcctg cccagcctca cgttcgcccg ggagctgcac ctctcagacg
    49621 acacgctgac gatcgagcgg cagaccgcgg cgggctacga cgtcgtggtg gccccaatcc
    49681 cggtcgtcat cacggtgacc gcgggggcca ccgagccccg ctacccgtcg ctgaagggcg
    49741 tgatggccgc gaagtcgaag ccggtcgaga cgctgaccat ctcggatctc gggctgtccg
    49801 gagacgacgt gaccgcgacg cagacggtgg ccgaggtcaa cgacgccccg ccgaagcagg
    49861 gtggtgagac cgtcgagttc gacgagacca ccgccggccg gatcgcggac ctcctcgccg
    49921 aggcgaaggt gatctgacat gaagatctgg gtgtgggccg acgtcacgca ggacgggccg
    49981 gcgccgtccg cgctcgagct cctgacgaag gcgcgcgagc tcggcgacga cgtggccgcc
    50041 gtggccctcg gtcccggggc gaccgcctcc gccgacgcgc tcggcgagca cggcgccacg
    50101 aaggtgttcg cctccgacga cgaggtgttc tcgaaccacc tcgcgcagcc tgctgttcac
    50161 gcgctcgccg gcctcgtgga ggagcactcc cccgacctga tcctgttccc cgcctcctac
    50221 gacgcccggg acgtcgccgg gcgcctgcac gcccgcatcg gctcgaccct gatggcgaac
    50281 gccacggacc tgttcggcgc ggacacggcc cggacggaga tcctcggagg cacgaaggtc
    50341 gtcgacgtcg agctgaaagg cccgacaccg aggctcgtga tcgtccggcc gcagtccttc
    50401 gcggccgagc cgtcgggcgg gtccgccgag atcgtggagg tgaaggtgga cgtcccggac
    50461 gagctcacga cggccgaaag gaccgaacgg cacgaggagg cggcgagcgg accgcgcctc
    50521 gagagtgcga aggtcgtcgt cgccggcgga cggggtttgg gcggaccgga cgcgttcgcg
    50581 cagctgggta cgctcgccgc cgcgatcggg aacgccgccg tcggcgcctc gcgtgcggcc
    50641 gtcgacgccg gctgggtgcc gtacagctac cagatcggcc agaccggcaa gaccgtgaag
    50701 cccgaggtct acatctgcgc cgggatcagc ggcgcgctcc agcacctcgt cggcatgaag
    50761 ggggcgaagc ggatcgtggc gatcacgaag gatcccgacg cgccgatcct gaagatcgcg
    50821 gacctgggcg tggtgggcga cctgttccag gtcgtcccgg ccctcaccga ggagatcgag
    50881 aagcgacgga cgggctcgta gccgctgcga tcctggcggc cgtcgtcgcc gtggcgatca
    50941 tgctcttcgt gcggcgcgtc cggccccgcg tggcgctcgt cgcgaacgcc cgtccggccg
    51001 ggcgaggtgc tcccaccgcc gatcgcgtca cccgcgagct cacccacgtc ctcctccaga
    51061 cgaagctgct ccagaagctc ggccccgggc tgatgcacgc gttcatcttc tgggggttca
    51121 tcgtgctgct cccgacgatc gccgaagccg cggtggcgat cgtcgacaag gactcgtcgc
    51181 tcccgctgct cggcggcgcg tcctggttcg cgttcctctc ggacgtcttc gcgaccctcg
    51241 tcgtcgtcgg catcgcgatg gcgttcttca tccgcaaggt cctgaagccc gatcgcttcc
    51301 gcgggagcca catgggcgag gccgaccgga tcctgctcct gatcctcgcg atcgtcgtga
    51361 cgttcctgct ctggaacgcg tcgcggatcg cgctcgggat cgccgaggtg ccggactcac
    51421 ggccggtcgc caatctgttg tccggctcgt tcggatccgg aggcggcacc gatatcgccg
    51481 agcgcatcct cgtgtgggcg cacctcctcg tgatcctcgg cttcctcgtg ttcttgcccg
    51541 gttcgaagca cctccatatc gccaccgcgc cggcgaacgt ctggctcgcc aaggacggcc
    51601 cgaccggtcg gctcgagccg aaccggatcg acctcgaggc gcccgaggag gacatccgct
    51661 tcggcgtcgc gacgcagaag gacctctcga agaagcagat gctcgacctg ttctcgtgca
    51721 ccgagtgcgg ccgctgccag gaggcgtgcc cagcctgggc gaccggcaag ccgctctcgc
    51781 cgaagctgct gatcatggac ctccgcgacc gcgtggtcgc cgaggccgcc ggcgaggtcg
    51841 agctccaacc gctcgtcccg aacgccgtga tcgaccaggt cgtctgggac tgcgtgacgt
    51901 gcggcgcgtg cgtgcgcgag tgcccggtcg acatcgagca cgtcgacacg atcgtcgacc
    51961 tccgccggaa cctcgtgatg gccgagtccc ggttcccgca ggaagcctcc ccgatgctcc
    52021 gcggcgtcga gaaccaggag aacccgtggg gccagccggc gtcggcgcgg ctcgactggg
    52081 cgaaggacct cgacgtgcgg gtgctccagg agggcgaccc ggcgccggag gtcctgttct
    52141 gggtcggctg cgccggcgcg ttcgacgagc gcgcccagaa gacgacgcag agcgtggcca
    52201 ggttgctggc cgccgcgggg atcgacgccg cggtgctcgg gccccgcgag aagtgcacgg
    52261 gcgatccggc gcgacggatg ggccacgagt acctgttcca gatgctcgcc gagcagaacg
    52321 tcgagacgct gaacggcgcc ggcgtgagga agatcgtcgc gagctgtgcg cactgcttca
    52381 acaccctcgc gaacgagtac cccgattacg gcgggacgta cgaggtggta caccacacgc
    52441 agctgctgtc cgaactgatc gcagccggcc ggctccagcc gcggacgggc gacgggaaga
    52501 tcacgtacca cgacgcctgc tacctcggcc gccacaacgg cgagttcgac gccccgcgct
    52561 cggtggtcgg cggcgagatc gaggagatgc cacggaaccg tgagcggtcc ttctgctgcg
    52621 gcgccggtgg cgcccggatg tggatggaag aggacgcgcc gaggatcaac gacacgcggt
    52681 tcgaggaggc cgccgcaacg ggagctgaga cggtggcgac cgcgtgcccc tactgcctcg
    52741 tgatgctcga tgacgccgcg aagggcaagg cgtccgagat ccgtatcgcg gacgcggcca
    52801 cgttgctggc cgagtcgaca ctcgactgag aaggtcgatg gacatgcaca ccgcggcccg
    52861 cgcgtggatc gaggcctggg gaggaaggct ggccgaccat ggacgtcgac cggatcgcgt
    52921 cccggtacca gcccgacgct ccctaccgct cgcatccgtt tcgcgacgtg tccaccgcgc
    52981 gcggatacat caccgaggcg ttcagcgagg aggagctcgt ccgctgttgg ttcggcgagc
    53041 cggtcgtgga tggcgatcgc gcggcggtcg agtactgggc gatcctgcga tcgcctgacg
    53101 ggggcgacgt cacgatcgcg gggacattcg cacctccgct tcgacgagaa agacctggtg
    53161 gtcgagcatc gggacgactg gactcagcgg gacggcgcgg ttgagccacc ggctggttgg
    53221 ggaacggcct gaacgatcca tcgggtcgag atcaccgcgc ccgcacggag agggcggccc
    53281 ggcccaccac cagcgcacgg gcggccgccg ccaataggac gagtacccat ccgacggagc
    53341 ccgccgtcga gaagccgggg gcggcggcag aacccgcgac gccggacgca acgatgagtg
    53401 cgacgcctgc gttccacgcc agcgcccgtg ccgcggccca cgcctccgtg cgcgtgtacg
    53461 ccgtcgccgg atcgaggcgc aggagtctcg gcgcgacgta cccgagcgac acgacgatcg
    53521 cctgcccgag gacgcccaac agcatcgccg cccccaccgg ctccagcagg cgccagccgc
    53581 ccgccgccac gacgaggacg tccgaccacg cggcgaacac gaaccaggcg gctactgcga
    53641 ggacggacca tcgttccggc gctttcgacg acgtgctcgc cctgaccacc gggacggaca
    53701 cgaccgtgac cgaccatgcg aacgccgcaa gcccggccgc ggcgatcccc cgcgcgaccg
    53761 ctccaggcgc cccaccgaac ccggtcccga cgagcccgag caccccgatc ccgagcccgt
    53821 acgcgccgat ccgcaaggcc cgagccgcgc ggacgtccgc gccttcccgg atcctcgtgc
    53881 gggcgatcgt gggaccgaag aagacgatcg tcgcgagcaa cgtgagtccg gcccagccaa
    53941 ggaggttcac gtggaggtga ccggtccgtg ccgcgccgac ccatccatcg gcgaggacgc
    54001 ccgtcccgac gagcgcaccc agcagcgcgc cgaacaggaa cgcgccgtgc gcccgttcgt
    54061 acgtccggac gacccagcgg aaccgggcac cgagcgacgt ccgacggaga cggcgaagcc
    54121 gccaggagga gacgaatacc accccggaga tcacggtcgc gccggccgcg accaaccacg
    54181 ggcggccgtt cgcgacaccc acgaagacag cgaccgcgcc gacgttggcc gcgatcagct
    54241 gtgcgatcgg ggcgtccccc ggttgacgcg tcagcgtcct cgcgaaatgg tcgctgagcg
    54301 ccaggatcag gttggtgacg acccccaggg tgaacaggtg cacggcgagc catcgaccac
    54361 cgggaagctc ggtgccgaac gcgatccaga tgacgacggc cagcgcgtgg accgcggcga
    54421 ccgccgccgc cggccggagc cggtcgaacg gaccgcgtcg agcgtcggct gtcatcgaac
    54481 gacggtgctc ttcagcgtct gaaagggcct gccggtcgac gagctcccgg acgtggcccg
    54541 cgagcgtgtg acccgcaccc tcacggagga ggagtggtag tacctccacg agagctgctc
    54601 gccggcgacg tagcctctcg gcccgctgat cacgacggtg tggttcttct tcctcgctca
    54661 ggatcaggtg aacgctttga gccccgtgag ctgctccccg atcacgagcg tgtggacctc
    54721 ctcggtcccc tcgtaggtga tcacggactc gaggttcgtc atgtggcgca tgatcgggta
    54781 ctcgagcgtg atcccgttcg ctccgaggat cccgcgtgag gtccgcgcga tctcgagcgc
    54841 ctcgcgcgtg ttgttcagct tcccgacgct cacctgctcc ggcttgagac ccttctcgtc
    54901 cttcagccgg ccgaggtggt acgcgagcag cgtccccttg tagagctcga gcgccatgtc
    54961 cgtgagcttc ttctgcgtga gctggaagga gccgatcggc ttgtcgaact gctcgcgggt
    55021 cttcgagtac tcgagcgccg tctcgaagca cgagcgcgcc gcacccatca cgccccaaac
    55081 gatcccgtag cgcgcctcgt tcaggcagga gagcgggcca cgcagcccgc ggacctcggg
    55141 cagcatcgcg tcagccggca accgcacgcc atccatcacc agctccgacg tcaccgaggc
    55201 ccggagcgag agcttcctgt ggatgtcgtt cgcggtgaag ccgggcgaat cggtcggcac
    55261 gatgaaccct cggaccccgt cgtcggtctg cgcccacacg atcgcgatat cggagcagcc
    55321 gccgttcgtg atccacatct tcgtgccgtt caagatccag tcgtcgccgt ccttcttcgc
    55381 ggacgtacgc atcgagcccg ggtcgctgcc cgagtccggc tccgacagac cgaagcacgc
    55441 gatcgccacg ccgctggcca tcttcgggag ccactcgttc ttctgctcct cgctcccgaa
    55501 cgcatggatc gcgtgaccgc acaaggcgct ctgcaccgaa acgaacgacc gcaggccgct
    55561 gtcgccggcc tcaatctcga gttccacgag tccgtacgcc acggagttca gtccgagcgt
    55621 ccactcggta tcgagctgca tcaggaagaa gccgagctcg ccgagctcct tcgcgagctc
    55681 gcgtgccggg aactcgccct tctcgtacca gtcctcgacc tcggggagga tccgacgttg
    55741 cacccactca cgagcgacac cgcggatctg cttctcctcg tcggagagga ggtggtcgat
    55801 gtcgatcaga tccgtgggct gtaggtcaga gggactcacg actccgaacg taccaccgga
    55861 gccgaaggcc accgtgacta ctccgccggc ttctcccaga ccgacacgtg ggatcggctc
    55921 tcgctcgt
//
LOCUS       DASUYD010000149        12301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1844031, whole genome shotgun sequence.
ACCESSION   DASUYD010000149 DASUYD010000000
VERSION     DASUYD010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12301
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1844031"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF195_08315"
     CDS             complement(<1..236)
                     /locus_tag="VF195_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580861.1"
                     /translation="MRPVPISLACTISDRTRPILDGRVPIEGCEVKAITGQPEDLFAR
                     ALRRQEFDVTELSLSSYLVVLARATSPYIAVPVFP"
     gene            complement(289..801)
                     /locus_tag="VF195_08320"
     CDS             complement(289..801)
                     /locus_tag="VF195_08320"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX6580862.1"
                     /translation="MGSIPLITRSEGLDADARSAYERIVESRGELTLPFQVLLHSPAI
                     AAQVAELGHVIRFGSHLSDADRELVTLATGRSIGCAFVWESHLAAAIATGVRQEAIAA
                     LESGPRRHGQREGILVAFVDELCSTDAVSDATFAAALDLLGIPGTVDLVSTVGYYTML
                     GRIMGACGMC"
     gene            complement(801..1145)
                     /locus_tag="VF195_08325"
     CDS             complement(801..1145)
                     /locus_tag="VF195_08325"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX6580863.1"
                     /translation="MSQLGHRVKDISDTGAHVAAIAVTATFSQMAFAEHLGVDLPLLS
                     DWGGATADAYGVRYDVWKGHEGLAKRSLFVIDREGVIRYRWVTEDALVLPDLDEPLEV
                     LQGLGRRREEEG"
     gene            complement(1155..1304)
                     /locus_tag="VF195_08330"
     CDS             complement(1155..1304)
                     /locus_tag="VF195_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580864.1"
                     /translation="MTRSDAPEVGDRAPEFRLRRSFEESVSLSDLLADGPLLLAFYVF
                     DFGPV"
     gene            complement(1310..2017)
                     /locus_tag="VF195_08335"
     CDS             complement(1310..2017)
                     /locus_tag="VF195_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX6580865.1"
                     /translation="MEGLEIEQQGATIVATIERGDQNSFSSEMIDALADAIAEATQDG
                     STRFVRIRARGPVFCVSRDRAGTTPEELRAEAERIVRINETLRTTPLISIAEVQGDAA
                     GFGGGIVAACDIAVASEEARFWFPEIAIGLAPTVVISWASKLLPTKRAFDMVVTGRPI
                     DAATAASVGLITEAVPSGQVAARVEARIAGMEALDAASVREIKRFFVHVRTMDAPSAA
                     QASIAPLVLGSLRLQGR"
     gene            complement(2041..2841)
                     /locus_tag="VF195_08340"
     CDS             complement(2041..2841)
                     /locus_tag="VF195_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017729107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6580866.1"
                     /translation="MDLRLEGRRAVVTGGSRGIGRAVATKLAAEGVRLAICARTQEPL
                     ESAAAQIADRTGSEVIPVVADVRDAASVEAFVATAAERLGGLEIVVSSAARVGAGSVP
                     EDLEHVAEGTILGDFEEKFLGALRICRAALPHLRQAGWGRIVTISGLTARVAGQVSAG
                     ARNAALVHLTRTMSVELGRDGITVNAIYPAVTVTEGLTERLTARAEREGTSSEQLLTN
                     LSKKSAIGRLVTADEVADVVVFLASERSAGITGEAIAVSGGTGVSVSY"
     gene            complement(2825..3250)
                     /locus_tag="VF195_08345"
     CDS             complement(2825..3250)
                     /locus_tag="VF195_08345"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6580867.1"
                     /translation="MTGLEVGLTIVAGVLTGILSAVFGTGGGQISIPFMILVLGFSQH
                     LAEGTSLFIIVPTAAVGAWAHSRRGYVRWRPAFWLSIAGLGGAAVGALLAVRLDELLL
                     RRFFAAFVLYAAYKFLRPKKRAAATKEDVSAEESDGSEA"
     gene            complement(3247..3636)
                     /locus_tag="VF195_08350"
     CDS             complement(3247..3636)
                     /locus_tag="VF195_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX6580868.1"
                     /translation="MGEASPPVSQPAYTLPALVAIGAATGLLSGLLGIGGGTVAVFGL
                     AVLMHWPQHPAHATALAAIPPLAAVGAIVFAANGHVDLRAGFLLIAGGFAGALIGARI
                     MARMPELLLRRAFGILMLAVGIRLLLP"
     gene            complement(3734..4108)
                     /locus_tag="VF195_08355"
     CDS             complement(3734..4108)
                     /locus_tag="VF195_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580869.1"
                     /translation="MTRRSASGIATLGRDPAPPERIGRWVALIVGLLFGAQLVEDETR
                     AGRASGIGMTLGFAGAIVMPPAFGATVEATGSHAAPFLAVAVGVGAASIGALCLAGRS
                     TGAGPMRSGGRSTARRPPSPAG"
     gene            complement(4113..4802)
                     /locus_tag="VF195_08360"
     CDS             complement(4113..4802)
                     /locus_tag="VF195_08360"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6580870.1"
                     /translation="MALVDLSQDIYQGMMVYPGHLKTVLFDHVSHEETAPRFTSGFSF
                     QTKGFLLNDNGPTHVDSFSHLDPDPSALTIDQMSLDLFYGDAICVDVTGAEPRTDIRV
                     SRVEEGLASAGLELHPGDIILFRTGTYERHGQTKAYLSEFPGLGADVANWIVERKVKA
                     FGVDTCTPDNPSSTIYPIHMMGRRHHITHYENLANLDRVVGTRFTFVGFPLKLQGAHG
                     GPTRAVAIVPD"
     gene            complement(4816..5577)
                     /locus_tag="VF195_08365"
     CDS             complement(4816..5577)
                     /locus_tag="VF195_08365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6580871.1"
                     /translation="MVVVTGAGRGIGREIALAFARAGSNVVLAGRDPATLKTTAIDAQ
                     SHGATALVEPTDVTEPDQVDRLAGRTLDAFGRVDGLVANSGIGGPSGPLWTLTPQEWD
                     STFAVNVTGVFLCCKAFLPPMLERGAGAVVVMGSMTGKRPLWGRSPYAASKLALVGLV
                     RTLALEAAPSGVRVNLISPGTVAGERIEWVFRSQAEGRGIPVERVREEFLAQIPLGRL
                     VEPEEVADATVYLTLHAPPAITGIDLNVSAGMVTY"
     gene            complement(5628..6944)
                     /gene="hisD"
                     /locus_tag="VF195_08370"
     CDS             complement(5628..6944)
                     /gene="hisD"
                     /locus_tag="VF195_08370"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEX6580872.1"
                     /translation="MPRYVKRPNGISDESRREVVRTVSEILGAVEHEGIDAVRRFSAR
                     FDGWSPPEFRVGSDEIDRATTSIPDELKAHIAFTQEQVRNFAELQRSTLVDAEWETLP
                     GVTLGQRFIPVESVGAYSPGGLYPLIASSIMTVAVPRVAGVGRIVGCAPPRDAGGMHP
                     PQLYAMATSGADEIFCIGGVQAFAAMAFGIEGLEPVDMLVGAGNAYVAEAKRQLFGTV
                     GIDLLAGPTEVMVLADDSADPEVVAADLLGQAEHGPTSPATLLTTSPELAEAVARAVE
                     HWLEGEWPTTEVAGVAWRDHGTIVVCEDEEEAASVADDMAPEHLEIHTGDPDWYLKRL
                     GSYGTLFLGTHATVAYSDKAVGTNHILPTRRAARYTGGLWVGKFLKTVTYQRLTPEGS
                     ARVAPTAAAISDAELMFGHALTARLRLDPVRFPDAMHAIPPEGVGS"
     gene            7026..8009
                     /locus_tag="VF195_08375"
     CDS             7026..8009
                     /locus_tag="VF195_08375"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX6580873.1"
                     /translation="MLTGPGQIELRDVDEPTAGSGETLVRIRAVGICGTDLSIFKGKI
                     PVTYPRIMGHEMVGEVLVSPGGSPGPGTRVIVDPNVACRRCDRCREGRANLCAAGWLL
                     GRDRDGALCERVVAPAENLYRVPEDLQDHLVPLIQVLTTCVHGQRLVPIFPGDGTVVV
                     GLGVTGLLHVQLAKLRGADPVVGITRSPRALELASELGADVTIAADDPAVEDRVKEAT
                     GGGGDVVIEAAGTVATLGRAVRVARSGGRVLAYGTISERGGDFPYYDLYYKELVLSSP
                     RAAQAEDYPVAIGAIASGSVRLDPLISRRVGLVGLSKTLDDPVGLKTIVEL"
     gene            8137..9180
                     /locus_tag="VF195_08380"
     CDS             8137..9180
                     /locus_tag="VF195_08380"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580874.1"
                     /translation="MRRNVGFRGAAALLMVFALVAVACGDDDGGGGGGGATGEEGVIR
                     FTFAPDPVWDYLKDSGIREEMEEESGIRILEQSTWDEFGIYAGGHADVVSIASFEVPL
                     LEEETGRPATVFGKYNIDRSVLVVPADSDAQTLEDLQGGKIAVWDAVSSTVIWGVLAN
                     EMYGLDFQTGGGDFELVLTDLGNTGALAANHEVDGALVLPDFNVPPLLNGDIRVLYDG
                     RTAADLYAEVAGAPDHEGPMINIFMAPTDWYDSHPDEVAFFLELWERGIQEWQANQDT
                     IIESYPQHFAVETPEEIQFMKDYLAAHDWFVETVYLDDAWVETESLIFPMLADAGLSE
                     SEESPRFDVVPAP"
     gene            9209..10033
                     /locus_tag="VF195_08385"
     CDS             9209..10033
                     /locus_tag="VF195_08385"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580875.1"
                     /translation="MANPATAIDPRAGGGLSVAGRRRVRGLLGFVVGVGGFLLLWALL
                     SRFIVGPFLLPPPTEIGAEMWRILSSGEFFDQFVPTITRLAYGFGLALLIGFPVGYLM
                     GTSRYWRAFFHDMVMVAGSIPGITYAVMSLVVLGISLLGPVVSVGLVAMPYIAINVAE
                     GLDSVDRRLVQMSDAFGRNRSRVVRHVLVPSVAPFAFAGVRLSFALAWKVEQLTEVFG
                     SSSGIGFQIRVEFEDFSIPGMLAWVLLFIIFMLLIERFGLVRLERYLFRWRLEDQA"
     gene            10030..10914
                     /locus_tag="VF195_08390"
     CDS             10030..10914
                     /locus_tag="VF195_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6580876.1"
                     /translation="MSVAIERPATPGGAPGAVRRALTSDTGARIASYLSLLLLWQLIA
                     MYEDRFPGPGEVFGFLWDEVTAGSHGGVVQGEFLEHFAATLPRFSFGIGVSLAVGLVV
                     GVLIGASKFADAVLKDTLLVLLALPAIIWAFLTQMWFGLGDRAVVITIVLSAFPFVAV
                     NIVQGVHAIPRELRRMTSAFHVTTYRTFRHLQVPAVTGYFFAGLRFAVIVGWNALLLA
                     EWFSSSEGVGWRSRYWYDANRYRGFIAWVIIFILFIVLLDRLVLVRLQRRAFRWRDAH
                     LTEIEREVEEVDIGVRGA"
     gene            10934..11719
                     /locus_tag="VF195_08395"
     CDS             10934..11719
                     /locus_tag="VF195_08395"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580877.1"
                     /translation="MATLKVDRLSKVFPAIERGSSPTTAIDELSFELSGHVFVSVVGP
                     SGCGKSTLLNIVSGIEAPTSGAVDVAEGDRPARLGYVFQDPRLLPWRSVLDNMLYVLD
                     GHGEGARERARSYLGMVGLEDAGGKFPSQLSGGMQQRVGIARAFSVEPDLLLMDEPFS
                     HLDAITARALREQLQEIWVQTQKTVLFVTHDVMEAVQLSDRIVVLAPGGRLYADIPIE
                     LPRPRRASDTDVATAQAQILQRFEAMQEEVERMHGATDVSARS"
     gene            11716..>12301
                     /locus_tag="VF195_08400"
     CDS             11716..>12301
                     /locus_tag="VF195_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003898298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6580878.1"
                     /translation="MIEQTGTERSRADDLLDTIEIPRRLLIGGEWLDAASGDELPVID
                     PATGRELTRVACAGSADVDRAVAAALATFEEGAWSRMPIAERARALQRFADTIEARIE
                     DLYRIESVNNGRPITETRAQLSRLPEWYRYNAALLLADRTDVISMPGPYHTYTTRFPI
                     GVAGILSPFNHPMMIGSKSIAPALATGNSVVLKPS"
BASE COUNT         1865 a         4230 c         4264 g         1942 t
ORIGIN      
        1 gggaacaccg gcacggcgat gtaggggctg gtggctcgag ccagcaccac gagatagctg
       61 ctgagcgaga gctccgtgac gtcgaactcc tgtcgccgca gggctcgcgc gaacagatcc
      121 tcgggctgcc ccgtgatcgc ctttacctcg cacccctcga tgggaactcg gccgtccagg
      181 atcggcctcg ttcgatccga gatcgtgcag gcgagcgata tcggaaccgg tctcaccgcg
      241 catccgatcc ggccaagcga agttcgacct catggccatc gcccgtcatc agcacatgcc
      301 gcaggcgccc atgatccgcc cgagcatcgt gtaatagccg accgtcgaga caagatcgac
      361 cgtccccggt atgccgagca gatccagagc ggcggcgaac gtcgcatccg agacggcgtc
      421 cgtgctacag agctcgtcga cgaatgcgac gaggatccct tctcgttgcc cgtgccggcg
      481 cggaccactc tcgagcgccg cgatcgcctc ctgccgaacc cccgtcgcga tcgcggccgc
      541 caggtgcgac tcccacacga acgcgcaacc gatggatcgt cccgtcgcca gggtgacgag
      601 ctcacggtcc gcgtcggaca ggtgggatcc gaaccggatg acgtgaccca gctccgcgac
      661 ctgcgctgcg atcgctggcg aatgcagcag cacctggaac ggtagcgtga gctcaccccg
      721 gctctcgacg atgcgctcgt acgccgatcg cgcgtcggcg tcgagccctt cgctcctggt
      781 aatcagcgga atcgaaccca tcaaccctcc tcctcgcggc gtcgacctag gccctggagc
      841 acctcgagcg gctcgtcgag gtcgggaagg accagggcgt cttcggtcac ccaccggtag
      901 cggatgacgc cctcgcggtc gatgacgaag agcgagcgct tcgccaggcc ctcgtggcct
      961 ttccatacgt cgtaacggac cccgtacgcg tcggcggttg ccccacccca gtcggacagg
     1021 agcgggagat cgacgccgag gtgctcggcg aacgccatct gcgagaacgt tgcagtgacg
     1081 gcgatcgccg cgacgtgcgc gcccgtgtcc gagatgtcct tcaccctgtg tcccaactgg
     1141 gacaactctt ccgttcagac cggtccgaag tcgaagacgt agaacgcgag caggagcggg
     1201 ccgtccgcga gcaggtcgga cagggatacc gactcctcga acgagcgtcg cagcctgaac
     1261 tccggtgctc ggtcgcccac ctcgggagca tccgatcggg tcatctccgt cacctccctt
     1321 gcaggcgcag cgacccgagg accagtgggg cgatcgacgc ctgggccgcg gacggtgcgt
     1381 ccatcgtacg aacgtgcacg aagaagcgct tgatctcgcg gacgctggcg gcgtcgagtg
     1441 cctccatgcc cgcgatccga gcctcgacgc gtgctgcgac ctggccgctc gggaccgcct
     1501 ccgtgatcag gccaacggat gcggcggtgg cagcgtcgat cggccggccg gtcaccacca
     1561 tgtcgaacgc ccgcttggtc gggaggagct tgctcgccca gctgatcacg acggtggggg
     1621 cgagaccgat cgcgatctcc gggaaccaga accgagcctc ctcggaggcc accgcgatgt
     1681 cgcaagcagc gacgatcccg ccgccgaatc cggccgcgtc accctgcacc tcggcgatcg
     1741 agatgagcgg cgtcgtccgc agtgtctcgt tgatccgcac gatccgctcg gcctcggcgc
     1801 ggagctcctc cggcgtggtg cccgcacggt ctcggctcac gcagaagacc ggtcctcggg
     1861 cgcggatccg cacgaaccgc gtcgagccgt cctgcgttgc ctcggcgatc gcgtcagcga
     1921 gcgcgtcgat catctcgctg ctgaacgagt tctggtcacc gcgctcgatc gtcgcgacga
     1981 tcgtggcacc ctgctgttcg atctccagac cctccatcgt tcctcctcga tccttcccgc
     2041 tcagtacgaa acggatacgc ccgtccctcc gctgaccgcg atcgcctcac cggtgatgcc
     2101 cgccgatcgc tcggacgcca ggaacacgac gacatcggcg acctcgtcgg ccgtgacgag
     2161 gcgaccgatc gcgctcttct tcgaaaggtt cgtgagcagc tgctcgctcg aggtcccctc
     2221 tcgctccgca cgagcggtga gacgctccgt gaggccctcg gtcacggtga cggcggggta
     2281 gatcgcgttg accgtgatgc cgtcgcggcc gagctccacg gacatcgtcc gggtgaggtg
     2341 cacgagggcg gcgttgcgcg cgcccgcgct cacctgcccg gcgacgcggg cggtgagccc
     2401 actgatcgtg acgatccggc cccatccggc ttgtcggagg tgggggagcg ccgcccggca
     2461 tatgcggagc gcaccgagga acttctcctc gaagtcgccc aggatggtcc cctcggccac
     2521 gtgctcgaga tcctcgggga cgcttcccgc ccccacccgg gcagcggagc tcacgacgat
     2581 ctccaggccg ccgagtcgct ccgccgcggt cgccacgaag gcctcgaccg atgccgcgtc
     2641 ccgcacgtcg gcgaccaccg ggatgacctc gctgccggtc cgatcggcga tctgggcggc
     2701 cgcggactcg aggggttcct gcgtgcgcgc gcagatcgcc agccgcacgc cctcggcggc
     2761 gagcttggtc gcgaccgccc tcccgatgcc tcggctccct cccgtcacga ccgcccggcg
     2821 gccctcaagc ctcagatcca tcgctctcct ccgcggagac atcttccttg gtggccgcgg
     2881 cccgcttctt gggccgcagg aacttgtatg ccgcgtacaa cacgaaggcg gcgaagaagc
     2941 gacgaagcag cagctcgtcg agccggacag cgagcagtgc ccccaccgcc gcgccgccca
     3001 gaccggcgat cgaaagccag aacgcgggtc gccaacgcac gtacccgcgg cgggagtgcg
     3061 cccaggctcc caccgcagcg gtcgggacga tgatgaacaa cgaggtcccc tccgcgaggt
     3121 gctgcgagaa cccgaggacg aggatcatga acgggatcga gatctgcccg cctccggtgc
     3181 cgaagactgc ggacaggatc ccggtgagta cgccggcaac gatggtgagg ccgacctcaa
     3241 ggccggtcat ggcagcagca ggcggatgcc gacggcgagc atgaggatcc cgaaggctcg
     3301 tcgcaggagc agctccggca tccgggccat gatcctggcc ccgatcaggg cgcccgcgaa
     3361 cccgccggcg atcagcagga agcccgcccg caggtccacg tggccgttgg ccgcgaacac
     3421 gatcgcgccg accgcggcca gaggcgggat cgcggccagg gcggtggcgt gcgccgggtg
     3481 ctggggccag tgcatcagga ctgcgagccc gaataccgct accgtccccc cgccgatccc
     3541 gagcaatccc gacagcaggc ccgtcgctgc cccgatggca acgagcgccg gcagcgtgta
     3601 cgcgggctgc gagaccggcg gcgacgcttc tcccaaaggc tccctcctgg atggaagatt
     3661 gcacactata tgcggttggc ccgggttgac cgtggcggcg gcgacccgtc cagggcctga
     3721 cgcagggtga gcgtcacccg gccggcgacg gagggcgcct cgccgtcgag cgccccccgg
     3781 aacgcatggg acccgctcct gttgaccgac cggcgaggca gagggcgccg atcgacgcgg
     3841 caccgacccc cacggcaacg gccaggaagg gcgcggcgtg cgaaccggtg gcctcgaccg
     3901 tggcgccgaa cgcgggcggc atgacgatcg cgccagcgaa ccccagcgtc atgccgatcc
     3961 cggatgcgcg gccagcgcgc gtttcatcct cgaccagctg cgcgccgaac aggagcccga
     4021 cgatcagggc aacccaacgc ccgatccgct cagggggtgc cgggtctcga ccgagcgtgg
     4081 cgataccgga cgccgagcga cgcgtcacgg cctcagtcgg ggacgatcgc gacggcgcga
     4141 gtgggacctc cgtgcgcgcc ctggagtttc agcgggaacc cgacgaaggt gaaccgcgtg
     4201 cccacaaccc gatcgaggtt ggcgaggttc tcgtagtggg tgatgtgatg ccggcggccc
     4261 atcatgtgga tcgggtagat cgtgctcgag gggttgtcgg gcgtgcaggt gtcgaccccg
     4321 aacgccttca ccttccgctc gacgatccag ttcgcgacgt cagcgcccag tcccgggaac
     4381 tcggacaggt aggccttcgt ctggccgtgg cgctcgtacg tccccgtgcg gaacaggatg
     4441 atgtcgccgg ggtgcagctc cagcccggcg gacgccaggc cctcttccac gcgggacacg
     4501 cggatatcgg tccgcggctc cgcacccgtc acatcgacgc agatcgcgtc cccgtagaac
     4561 aggtcgagcg acatctggtc gatcgtgagc gcggacggat ccggatcgag gtggctgaac
     4621 gagtcgacgt gggtgggacc gttgtcgttc aacaggaagc ccttcgtttg aaacgagaac
     4681 ccgctcgtga accgcggggc ggtctcctcg tgcgagacgt ggtcgaacag cacggtcttg
     4741 aggtgaccgg gatagaccat catcccctgg tagatgtcct gcgacaggtc gacgagcgcc
     4801 atccgttcct ccttcctagt acgtgaccat cccggcactg acgttcaggt cgatgcccgt
     4861 gatcgcgggc ggggcgtgca gcgtgaggta cacggtcgca tcggcgacct cctccggttc
     4921 gacgagacgc ccgagcggga tctgcgccag gaactcctca cgcacccgct cgacggggat
     4981 gccgcgtccc tcggcctggg agcggaagac ccactcgatg cgctcgccgg ccaccgtccc
     5041 cggtgagatc aggttcaccc ggaccccgga gggcgcggcc tcgagggcga gcgtgcggac
     5101 gagccctacg agcgccagct tgctcgccgc gtacggcgag cggccccaga gcgggcgctt
     5161 gccggtcatc gaccccatca cgacgacggc gcctgccccg cgctcgagca tcggaggcag
     5221 gaacgccttg cagcacagga agaccccagt gacgttgacg gcgaaggtgg aatcccactc
     5281 ctgcggcgta agcgtccaca ggggtccgga cgggccgccg atcccgctgt tggccaccag
     5341 cccgtccacg cgcccgaacg catccagggt ccgccccgcc aggcgatcga cctgatcggg
     5401 ctcggtgacg tcggtgggtt cgaccagcgc ggtcgcgccg tggctctgtg catcgatggc
     5461 cgtcgtcttc agcgtcgcgg gatcgcgccc ggcgagcacg acgttcgatc cggcgcgcgc
     5521 gaacgcgagc gcgatctccc gtccgatccc gcgtcctgcg ccggtcacga cgaccacgga
     5581 gcgtccctcc gaggaccggg gggcggaacc ggtcatctcc tgggcgctca gctgcctact
     5641 ccttcgggcg ggatggcatg catcgcatcg gggaagcgga ccgggtcgag gcggagtcgg
     5701 gcggtgagcg cgtgaccgaa catcagctcc gcgtccgaga tcgcggccgc tgtcggcgcc
     5761 accctggcgg acccctccgg ggtgagccgc tggtacgtga cggtcttcag gaacttgccg
     5821 acccacagac ctcctgtgta gcgagccgcg cgccgcgtcg ggaggatgtg gttggtcccg
     5881 acggctttgt ccgagtacgc caccgtcgcg tgcgttccga ggaacaaagt gccgtaggag
     5941 ccgaggcgct ttaggtacca gtccggatcc cccgtgtgga tctccagatg ctcgggggcc
     6001 atgtcgtcgg cgacgctcgc cgcctcctcc tcgtcctcgc agacgacgat cgtcccgtgg
     6061 tcccgccacg cgacgccggc cacctccgtc gttggccact cgccctccaa ccaatgctcg
     6121 accgcccgcg cgaccgcctc ggcgagctcg ggcgaggtcg tgaggagggt cgcgggcgag
     6181 gtcggaccat gctcggcctg gcccaggagg tcggcggcga cgacctccgg gtcggccgag
     6241 tcgtcggcca ggaccatcac ctccgttggc ccggccagca ggtcgatccc gaccgtgccg
     6301 aacagctggc gcttggcctc ggcgacgtag gcgttcccgg cgccgacgag catgtcgacg
     6361 ggctcgagcc cctcgatccc gaacgccatc gcggcgaagg cctgtacgcc gccgatgcag
     6421 aagatctcgt ccgcacccga cgtcgccatc gcgtacagct gcggtgggtg catgccccct
     6481 gcgtccctgg gtggcgcgca tccaacgatc cgcccaaccc ccgccacacg gggaacggcg
     6541 acggtcatga tcgacgatgc gatcaggggg tacaggccac cgggggagta ggcgcccacc
     6601 gactccacag ggatgaagcg ctggcccagg gtgacgccgg ggagcgtctc ccattcggcg
     6661 tcgacgagcg tcgagcgctg gagctcggcg aagttgcgga cctgctcctg ggtgaaggcg
     6721 atgtgggctt taagctcgtc ggggatcgag gtggttgcgc gatcgatctc gtccgagccc
     6781 acccggaact ccggcgggct ccacccgtcg aagcgggcgg aaaatcgccg aacggcgtcg
     6841 ataccctcgt gctccacggc gccgaggatc tcgctcaccg tccggaccac ctcgcgccgg
     6901 gactcatcgg atatcccgtt cggccgtttc acgtatctcg gcatggccca cctcctacag
     6961 atcgattgtg tattgtgtgc aggcttccgc gtccgacgct accgggggga tccctgcagg
     7021 ctgccgtgct gaccgggccg gggcagatcg agctccggga cgtcgatgag cccactgccg
     7081 ggtcgggcga gacgctggtt cggatccggg ccgtcgggat ctgcgggacg gacctcagca
     7141 tcttcaaggg gaagatcccg gttacctacc cgcggatcat ggggcacgag atggtgggcg
     7201 aggtgctcgt gtcccccggc ggaagcccgg ggcccggcac ccgggtgatc gtggacccga
     7261 acgtggcctg ccgccggtgc gaccggtgcc gcgagggccg ggcgaacctg tgcgccgccg
     7321 gatggctcct ggggcgcgat cgcgacggcg ccctgtgcga gcgggtggtc gcgcccgcgg
     7381 agaacctgta ccgggtcccc gaagacctcc aggatcacct cgtaccactg atccaggtcc
     7441 tcacgacttg cgtgcacggg cagcgcctcg tcccgatctt cccgggggac ggcacggtcg
     7501 tcgtcgggct cggcgtcaca gggctgctgc acgtccagct cgcgaagctc cggggcgccg
     7561 atccggtggt ggggatcacc cggagcccac gggcgctcga gctggcgagc gagctgggcg
     7621 cggacgtgac gatcgcggcg gacgatcccg ccgtcgaaga ccgggtcaag gaggcgaccg
     7681 ggggcggagg ggacgtcgtg atcgaggccg cgggcacggt cgccacgctg ggccgcgccg
     7741 tccgcgtcgc ccggtccggg gggcgcgtgc tcgcgtacgg gacgatctcg gagcgtggcg
     7801 gggacttccc ctactacgac ctctactaca aggagctggt gctctcgagc ccgcgggcgg
     7861 cacaggccga ggactacccc gtcgccatcg gcgcgatcgc ctcgggcagc gtgcggctgg
     7921 atccgctgat ctcgcgccgg gtaggcctgg ttggcctctc gaagacgctc gacgatccgg
     7981 tcgggctcaa gacgatcgta gagctttgac ccccccacga atggttgggt tgaccgtcca
     8041 ttgggtgggg ggtagcgtgg ggcaaacggc gcattatata caatgcgtcg gatcggtttc
     8101 ctgggggcga tcgccccgga ggaaggggga cggcggatga gacggaacgt cgggttccgt
     8161 ggtgcggcgg cgttgctcat ggttttcgcc ctcgtcgcgg tcgcgtgtgg cgacgacgac
     8221 ggcggcggcg ggggtggtgg tgccaccggt gaagagggcg tcatccgctt cacgttcgcc
     8281 cccgatcccg tctgggacta cctgaaggac tccggcatcc gagaggagat ggaggaggag
     8341 tccggcatcc ggatactcga gcaatcgacg tgggacgagt tcggcatcta cgccggcggg
     8401 cacgccgacg tcgtctcgat cgcgtcgttc gaggtcccgc tcctcgagga ggagaccggg
     8461 cgacccgcga ccgtcttcgg caagtacaac atcgaccgaa gcgtgctggt cgtccctgcg
     8521 gactcggatg cgcagaccct cgaagacctg cagggcggca agatcgcggt gtgggacgcc
     8581 gtgtcctcca cggtgatctg gggcgtccta gccaacgaga tgtacgggct ggacttccaa
     8641 acgggcggcg gcgacttcga gctggtgctc accgacctcg gtaacaccgg cgcgctcgcg
     8701 gcgaaccacg aggtcgatgg cgcgctcgtg ctgcccgact tcaacgtgcc tccgctgctg
     8761 aacggcgaca tccgggtgct ctacgacggg cggaccgccg cagacctgta tgcggaggtc
     8821 gccggcgccc ccgaccatga ggggccgatg atcaacatct tcatggcgcc tacggactgg
     8881 tacgactcgc acccggacga ggtcgccttc ttcctcgagt tgtgggagcg cgggatccag
     8941 gagtggcagg ccaaccagga cacgatcatc gagagctacc cgcagcactt cgccgtggag
     9001 acgcccgagg agatccagtt catgaaggac tacctcgcgg cccacgactg gttcgtcgag
     9061 acggtctacc tcgacgacgc gtgggtcgag acggagtcgt tgatcttccc gatgctcgcg
     9121 gacgcggggc tctccgagag cgaagagtcg ccgcggttcg acgtcgtccc ggctccatga
     9181 tccggtagcg gcgagcggcg taggcggcgt ggcgaacccg gcgacggcga tcgacccccg
     9241 cgcgggggga gggctatcgg tcgccgggcg ccgccgcgtc cgagggttgc tgggcttcgt
     9301 cgtcggcgtc ggcggtttcc tgctgctttg ggcgctgctg tcccgattca tcgtcggccc
     9361 gttcctgctc ccgccgccca ccgagatcgg cgcggagatg tggcggatcc tgagttcggg
     9421 ggagttcttc gaccagttcg tgcccacgat cacccggctc gcgtacggct tcgggctggc
     9481 cctgctgatc ggcttccccg tcggctacct gatggggacg agccgctact ggcgcgcctt
     9541 cttccacgac atggtgatgg tcgccgggag catcccgggg atcacctacg cggtgatgtc
     9601 cctggtggtg ctcggcatct ccctgctcgg gcccgtggtc tcggtgggac tggtggcgat
     9661 gccctacatc gcgatcaacg tcgcggaggg tctggactcg gtcgaccggc gcctggtgca
     9721 gatgagcgac gccttcggcc gcaaccgctc gagggtcgtc cgccatgtgc tcgtcccgtc
     9781 ggtcgcgccc ttcgccttcg caggggtgcg gctctcgttc gccctggcct ggaaggtcga
     9841 gcagctcacc gaggtcttcg ggtcctcgag cggcatcggg ttccagatcc gggtggagtt
     9901 cgaagacttc tcgatcccgg gcatgctcgc ctgggtgctc ctgttcatca tcttcatgct
     9961 cctgatcgag cggttcggcc tggttcgcct ggagcggtac ctgttccgct ggcggctgga
    10021 ggatcaggca tgagcgtcgc gattgagcgg ccggcgacgc ccggaggggc gccaggcgcg
    10081 gtccgccgcg cgctgacctc cgataccggc gctcgaatcg cgagctacct gtccctcctg
    10141 ctcctgtggc aactgatcgc gatgtacgag gaccggttcc cgggcccggg cgaggtgttt
    10201 gggttcctct gggacgaggt caccgcgggt agccacggag gcgtggtgca aggcgagttc
    10261 ctcgagcact tcgccgccac cctcccgagg ttctccttcg ggatcggcgt ctcgctcgcc
    10321 gtcgggctgg tggtgggggt gctgatcggc gcctcgaagt tcgccgatgc ggtcctcaag
    10381 gataccctcc tcgtcctgct cgccctgccc gcgatcatct gggcgttcct cacgcagatg
    10441 tggttcgggc tgggcgaccg ggctgtcgtg atcacgatcg tgctgtccgc gttccccttc
    10501 gtcgcggtga acatcgtgca gggggtgcat gcgatcccac gcgagctccg ccgcatgacg
    10561 tccgctttcc atgtgacgac gtaccggacg ttccgtcacc tgcaggtccc tgccgtcacc
    10621 ggctacttct tcgccggcct gcggttcgcc gtgatcgtgg gatggaacgc cctgctcctc
    10681 gccgagtggt tctcctcgag cgagggggtg gggtggcgca gccgctactg gtatgacgcg
    10741 aaccggtacc gtgggttcat cgcgtgggtc atcatcttca tcctgttcat cgtgctgctc
    10801 gatcggctgg tgctcgttcg gctccagcgt cgcgccttcc gctggcgaga cgcgcacctg
    10861 acggaaatcg agcgcgaggt ggaggaggtc gacatcggag tgcggggcgc gtgaggatcc
    10921 ggaggccgac gacgtggcga cgctgaaggt cgatcgcctc tccaaggtgt tccccgcgat
    10981 cgagcggggt tcctcgccga ccacggccat cgatgagctc agcttcgagc tgagcggtca
    11041 cgtgttcgtg tcggtggtgg ggccctccgg ctgcgggaag agcacacttc tgaacatcgt
    11101 gtcggggatc gaggcgccca catcgggagc ggtcgatgtc gccgagggcg accggccggc
    11161 ccggctcggg tacgtcttcc aggaccccag gctcctgccg tggcgctccg tcctcgacaa
    11221 catgctgtac gtgctggacg gccacggcga gggtgcgcga gagcgcgcac gctcctacct
    11281 cgggatggtc ggactcgagg atgcgggggg gaagttcccc tcgcagctct cgggcggcat
    11341 gcagcagcgg gtcggcatcg cccgggcgtt ctcggtcgaa cccgacctgc tgctgatgga
    11401 cgagcccttc agccacctgg acgccatcac ggcgcgtgcg ctccgcgagc agctgcagga
    11461 gatctgggtg cagacgcaga agacagtcct gttcgtgacg cacgacgtga tggaagccgt
    11521 gcagctttcc gatcggatcg tcgtgcttgc gcccggcggt cggttgtacg ctgacatccc
    11581 gatcgagctc ccccgtccgc ggcgcgcctc cgacacggat gtggcgacgg cgcaagcgca
    11641 gatcctccaa cggttcgagg cgatgcagga ggaggtggag cggatgcacg gagcgacgga
    11701 cgtctccgcc cggtcgtgat cgagcagacc ggcaccgagc gctcacgcgc ggacgatctc
    11761 ctggatacga tcgagatccc acgacggctc ctgatcggcg gtgaatggct cgacgccgcg
    11821 agtggggacg agctccccgt gatcgacccc gcgacgggac gcgagctcac tcgggtcgcg
    11881 tgcgcgggtt cggcggacgt ggaccgggcg gtcgccgcgg cgctcgcgac cttcgaggag
    11941 ggcgcttgga gccgcatgcc catcgcagag cgcgcccgtg ccctccagcg cttcgccgac
    12001 acgatcgagg ctcggatcga ggacctgtac cggatagaga gcgtcaacaa cggccggccg
    12061 atcaccgaga cccgtgcgca gctctcgcgg ctgccggagt ggtaccgcta caacgccgcg
    12121 ctcctgctcg ccgatcggac cgacgtgata tcgatgcccg gcccgtatca cacctacacg
    12181 actcgcttcc cgatcggcgt cgccgggatc ttgagcccgt tcaaccaccc gatgatgatc
    12241 gggtccaaga gcatcgcgcc ggcgctggcc accggcaaca gcgtggtcct caagccgtcc
    12301 g
//
LOCUS       DASUYD010000150         2484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_79719, whole genome shotgun sequence.
ACCESSION   DASUYD010000150 DASUYD010000000
VERSION     DASUYD010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2484
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79719"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..608
                     /locus_tag="VF195_08405"
     CDS             150..608
                     /locus_tag="VF195_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein FlaR"
                     /protein_id="HEX6580879.1"
                     /translation="MPVIHLDLHFWKPGWVAPSEPEWREKQSGLLAGEAWIADGNYHE
                     TLDLRLERADTVVFLDTPWWVCAGRAFLRGLRKPVGEMPEGCDDSSWRRLRDEWRLAI
                     VIWRTRRSELEREHEIISQYGQHAVLHALGSKRAAKEFLDGLDGRVHGGR"
     gene            831..1232
                     /locus_tag="VF195_08410"
     CDS             831..1232
                     /locus_tag="VF195_08410"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX6580880.1"
                     /translation="MSDRHGLLRALREPRSANELLLARLTVLVMLTVILVLAGAFAFY
                     RLERNAPGSEVTTYGDALFWTSSQITTISSSLANPISTGGRILAVLTDLVSIAVVSLL
                     FGTIAQHIHIVSPKRERSFHRTAEEDDAAEP"
     gene            complement(1926..2108)
                     /locus_tag="VF195_08415"
     CDS             complement(1926..2108)
                     /locus_tag="VF195_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580881.1"
                     /translation="MLLGGISVWGKYGGPTGGQFQRWLDRTITSAFPDLSSGRLSTTG
                     RFLAGNIGLWLLIALR"
     gene            complement(2312..>2484)
                     /locus_tag="VF195_08420"
     CDS             complement(2312..>2484)
                     /locus_tag="VF195_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015655521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HEX6580882.1"
                     /translation="RPVSIGSNVWIGAAAVVLPGVSVGDDSIIGAGSVVTRDIPSGVV
                     AVGSPCRVVRAL"
BASE COUNT          430 a          837 c          809 g          408 t
ORIGIN      
        1 tgctcagttg atgcgcctga tacagcactt gagggacact gacgtcgatg agcgatgcag
       61 ccttggggat aggccgccgc gtactcgtga ctgggatggc tgggtcgggg aagagcacct
      121 tctcgcgctc gcttgctgcg aagaccggct tgccggtcat ccatctcgac ctccatttct
      181 ggaagcccgg ctgggttgcg ccgtcggagc ccgagtggcg cgagaagcaa agcggcctgc
      241 tcgccggcga ggcgtggatc gccgacggca actaccacga gacactcgac cttcgcctcg
      301 aacgcgcgga cacagttgtg tttctcgaca cgccctggtg ggtgtgcgcg ggacgcgcgt
      361 tcctgcgcgg cctgcggaag ccggttgggg agatgcccga agggtgcgac gattcgtcct
      421 ggcgacggtt gcgcgacgag tggcgtttgg ccatcgtcat ctggcggact cgccgctccg
      481 agctcgagcg cgaacacgag atcatctcgc agtacgggca gcatgccgtc cttcacgcgc
      541 tcgggtccaa acgggcagct aaggagttcc tcgacgggtt ggacggtcga gtacacggag
      601 gccgatgacg gctgacgagc tccgaggttc cccctcatta ggttccccag cctccaggta
      661 catccgcgaa ccctcgggca tcctagtgtg gagtcccagg acacggctat gtgccccctc
      721 agctaccctc ccgtcccgga cggcttcagg tgtggtgtcg ccacatcggc caatcagatc
      781 gagggttcga cggtcgtgcg tcagtgatcg tcatccgcta gcttcgccct atgagcgatc
      841 gacacggtct cctccgagcg ctgcgcgaac cccgatcggc gaacgaactc ctcttagcac
      901 ggctgaccgt cctcgtgatg ctcacggtga tccttgtcct cgcgggcgcc ttcgcgttct
      961 atcgactcga gcgaaacgcg ccgggctctg aggtcacgac atacggtgac gccctgttct
     1021 ggaccagctc gcagatcacg acgatctcct cctccctggc gaacccgatc tcgaccgggg
     1081 ggcgcatcct cgccgtactg accgacctcg tctcgattgc cgtggtgtcg ctgctgttcg
     1141 gaacgatcgc ccaacacatc cacatcgtga gccccaagcg cgagcggtcg tttcaccgaa
     1201 cggccgaaga ggatgacgcg gccgagccgt gaatcgtcgt cggtgcgccg gagcgacagc
     1261 gtcgggatgt gccaccgcat actggatgaa tctcggcgat ggaccgaccg ctgccgagct
     1321 cgacgtgact ctatccctgc acacgtcata gaccccccat ctctcgtcag gcaacattcc
     1381 cgaacactcg ggcaccagcg ggtgctgttc atcgacgagt tcaccgagtt ccgccggcac
     1441 ctggtcgagg ggttgcggat gcgtgtatcc cgcggcggag cgcgattcga tcgcggcgcc
     1501 gcggtcccca ccgatgtcca ggtgcgcggt ccgtcccgat cgataggtca ccgggaagac
     1561 cgcttcggga gcgaagacac ggaagcccag gaaggggatc gagaatccga tgatgaattc
     1621 gatcccgatc tccgacggcg tatacgttcg ggtcaggatc gagccgaccg caaggaccgc
     1681 gcacaacgct accccgatcg cgagagcccg tcgacgcggc cgccccgcgg gaacgagtcc
     1741 gtagacgaga cccatgacgg tgacggcgag ggaggcagcc gcgtaggccg gatgcttcgt
     1801 gatgcccgac tcagcgatgc ccgcggtcgg gaaccatcgg acgctcaccg tcacgagcac
     1861 caccgatccc acgaagacgg cgaggtgccg ccagcgcctg tacgcgatca acgccaggcg
     1921 gtcccctacc ggagcgcgat caggagccag aggccgatgt tgcctgcaag gaacctgccc
     1981 gtcgtcgaaa gtcggccgct gctgaggtcg gggaaggcgg aggtgatggt gcgatcgagc
     2041 cagcgttgga actgaccgcc cgtcggtccg ccatacttcc cccagacgga gatcccgccg
     2101 agcaacaaga ccgtcaggat ccaaagggtg gcggtgccgc tgagatcgcg tggcagaggc
     2161 ggcggttctc caacgggccg ccgccgccga cgcgggcttg tacctggggc ggccgaccct
     2221 ccctcgacct tgtggggcca tccggtcctc cggcgtgatg ctacgccgct ctggtggagt
     2281 gggccatctc ggttgcgatc ccgagcagcc ctcagagcgc gcgcacgacg cggcacgggg
     2341 aacccaccgc caccacccca ctcgggatgt cgcgcgtgac cacgctgccc gccccgatga
     2401 tggagtcgtc ccccaccgag actcccggga gcacgacggc cgccgcgccg atccatacgt
     2461 tcgatccgat cgagacgggc cgag
//
LOCUS       DASUYD010000151         3452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1184067, whole genome shotgun sequence.
ACCESSION   DASUYD010000151 DASUYD010000000
VERSION     DASUYD010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184067"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..577
                     /locus_tag="VF195_08425"
     CDS             164..577
                     /locus_tag="VF195_08425"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580883.1"
                     /translation="MKAERLRKVPLFEGLTDHQYERLERWTDEIDVPTNKHLVNQGAY
                     PHEFMVIESGTAEVTHDGTHLADLGPGDFFGEMALLLNVPRTATVAATSDVTLVVMHD
                     RNFRAMEEEIPVIAQRVNAIMEERRAKSADQGIET"
     gene            complement(667..1095)
                     /gene="tadA"
                     /locus_tag="VF195_08430"
     CDS             complement(667..1095)
                     /gene="tadA"
                     /locus_tag="VF195_08430"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX6580884.1"
                     /translation="MRLALEEARAAASAGDVPVGAVVARGDEVVGRAGNAREREQDPT
                     AHAEILALRQAARALGSWHLEGCTLYVTLEPCAMCAGALVLARIDRLVLGAPDPKAGF
                     AGSLGDIVRDGRLNHEVEVTVGVLAEECGEVLRAFFAERR"
     gene            complement(1147..1494)
                     /locus_tag="VF195_08435"
     CDS             complement(1147..1494)
                     /locus_tag="VF195_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEX6580885.1"
                     /translation="MVDREHFEELVMEALDSLPAWVHERLENIEMIVEDRPPRDDPNL
                     LGRYHGVPLTKRGQFHSGPTFPDTITLYKRTIERVSRDDEHLRQVIAHTVEHEVAHFF
                     GISDDRLREIDRY"
     gene            complement(1497..2525)
                     /gene="speB"
                     /locus_tag="VF195_08440"
     CDS             complement(1497..2525)
                     /gene="speB"
                     /locus_tag="VF195_08440"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEX6580886.1"
                     /translation="MSEPPDFRLEQSELRSEDEFDEFGYEDLEYYVDFRGTFDKLPLC
                     PDDDSLAASGADVAIVGAPLDDGTSGRPGARFGPRAIRSAPTTWGSDAWSIQLQVEPY
                     ARLNIVDAGDAPVVASRHARGLRVIHEKVLRVASSGALPFVLGGDHSITYPSAAAVAR
                     HVWPRKLGVVHFDAHADAASSVGGSLISHGTPMRRLIEEGWVEGRNFVQVGLRGYWPE
                     KATFDWMAEQGFRWHTIVDVEERGVDAVIDDAIAEALDGPDLVYLSVDIDVVDPGMAP
                     GTGTPEPGGMSARELLRAIRRVARAVDLAGMDVVEVSPPYDQSEVTALLAYRVVMEAL
                     SALAARRG"
     gene            complement(2578..3315)
                     /locus_tag="VF195_08445"
     CDS             complement(2578..3315)
                     /locus_tag="VF195_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580887.1"
                     /translation="MLWLRRVRLLRSRSVLVLVVVAATVVSACDHGADAPVEEPRTRL
                     LTLEDLWFASPATITYRTTERDPGEATSPPQCLRQLVEVDVQTGLRICSGVGEMRLAW
                     DPPDRWRMDGASPDGGFTLLSTPDGDVRCRGTEVAASTCFATETSGPFASVVEAPERI
                     LAELGASGGSVVSAGPRRTIAGIPAECFSAIGDSAETSHRLEWCFSRGGLLLYLFDGV
                     EEGRVTTVEATDVSEVVADRDFVVPST"
BASE COUNT          572 a         1253 c         1118 g          509 t
ORIGIN      
        1 cgacaggatt cgaacctgtg aggggcggat taggccccta accgctttcg aggcggtccc
       61 gttcgtccgc tccggcacgc ctccggggcg agtctagcaa gcagcttcgg gccggcctgt
      121 gcgaggattt cggcgcggac cgcgaggaac ggaggatgcc ccgatgaagg ccgaacggct
      181 gcggaaggtc cccttgttcg agggcctgac cgatcaccag tacgagcgtc tggagcggtg
      241 gacggacgag atcgacgtcc cgacgaacaa gcacctggtg aaccaggggg cctatcccca
      301 cgagttcatg gtgatcgaat ccgggacggc cgaggtgacc cacgacggga cgcacctggc
      361 ggatctcggc ccgggtgatt tcttcggcga gatggcgctg ctgctgaacg tgcctcggac
      421 cgccaccgtc gcggcgacct cggacgtcac gctcgtcgtc atgcacgatc ggaacttccg
      481 ggccatggag gaggagatcc ccgtgatcgc ccagcgcgtg aacgcgatca tggaagagcg
      541 ccgagccaag tccgccgacc aggggatcga gacgtagggg atcgcgtttc ggcgagcgac
      601 cgatccccca cgaccggcga taccctcccc ggtatcgccg gccaccctcg ggcgacctgg
      661 accaacctag cgccgctcgg cgaagaacgc tcgtagcacc tcgccgcact cctcggcgag
      721 cacgcccacc gtcacttcca cctcgtggtt gagacgcccg tcgcgcacga tgtcccccag
      781 cgatccggcg aacccggcct tcgggtccgg cgcgcccaac accagccggt cgatccgagc
      841 cagcacgagc gcgccggcac acatcgcgca cggctcgagc gtgacgtaca aggtgcagcc
      901 ctcgaggtgc cagctcccca aggctcgcgc cgcctgccgg agcgccagga tctcggcgtg
      961 ggcggtgggg tcttgttcgc gctcgcgagc gttgcccgcg cgtcccacga cctcatcgcc
     1021 tcgggcgacg acggccccga ccggcacgtc acccgccgag gccgccgctc gagcctcctc
     1081 gagcgccagc cgcatcaacc ggtcgtccgg atcccccgaa gcgcccaccg ccgaggcgtc
     1141 caccggtcag taccggtcga tctcacgcag gcggtcgtcg ctgatcccga agaagtgcgc
     1201 cacctcgtgc tcgaccgtgt gcgcaatgac ctgtcgcagg tgctcgtcgt cccgcgacac
     1261 ccgttcgatc gtccgcttgt acagggtgat cgtgtcggga aacgtcggcc ccgagtggaa
     1321 ctgacctcgc ttggtcagcg ggacaccgtg gtagcgcccg agcaggttcg gatcgtcccg
     1381 cggcgggcgg tcctcgacga tcatctcgat gttctcgagg cgctcgtgca cccatgcggg
     1441 cagcgagtcg agggcctcca tcaccagctc ctcgaagtgt tcgcggtcca ccatcatcaa
     1501 cctcgccgcg cggccaacgc cgagagcgcc tccatgacca cacgataggc gagcagcgcc
     1561 gtgacctcgc tttggtcgta cggcggcgac acctccacca catccatccc ggcgagatcg
     1621 acggccctcg cgaccctgcg gatcgccctc agcagctcgc gggccgacat gccgccgggt
     1681 tcgggcgtcc ccgttccggg agccatcccg gggtcgacga cgtcgatgtc caccgagagg
     1741 tagacgagat cggggccgtc gagcgcctcg gcgatcgcgt cgtcgatgac cgcgtcgacc
     1801 ccgcgctcct ccacgtcgac gatcgtgtgc cagcggaacc cctgctccgc catccagtcg
     1861 aacgtggcct tctcgggcca gtacccgcgc aggccgacct ggacgaagtt ccgaccttcg
     1921 acccatcctt cctcgatcag ccgccgcatc ggcgtcccgt gcgagatcaa cgacccgccg
     1981 accgaactcg cggcgtccgc gtgcgcgtcg aagtggacca cgccgagctt ccgaggccac
     2041 acgtggcgcg cgacggcggc cgccgacggg tacgtgatcg agtggtcgcc gcccaacacg
     2101 aacggcaacg ctcccgacga agcgacccgc agcaccttct cgtggatcac ccgcaggcct
     2161 cgggcgtggc gcgacgcgac gacgggcgcg tcgcccgcgt ccacgatgtt cagccgcgcg
     2221 tacggctcga cctggagctg gatcgaccag gcatcgctcc cccaggtcgt cggcgccgaa
     2281 cggatcgctc gcgggccgaa ccgggccccg ggtctccccg acgtcccgtc gtcgagcggc
     2341 gcgcccacga tggccacgtc ggcgccgctc gccgcgaggc tgtcgtcgtc cgggcaaagc
     2401 gggagcttgt cgaacgtgcc tcggaagtcg acgtagtact cgagatcctc gtacccgaac
     2461 tcgtcgaact cgtcctcact gcgcagctcg ctctgctcga gacggaagtc ggggggctcg
     2521 ctcatcgcta cggcggacgc tacccgagcc ggcctcggag cgtcagtgtt ccctcaatca
     2581 ggtggacggc acgacgaagt ctcggtcggc cacgacctcc gatacgtcgg tagcctcgac
     2641 cgtcgtcacg cgaccttctt cgaccccatc gaagagatag agcaagagtc cgccccggga
     2701 gaagcaccac tccaatcggt gagacgtctc cgccgaatct ccgatcgcac tgaagcactc
     2761 ggcagggata cccgcgatcg tccgccttgg tcctgcggac accaccgatc cgccggaagc
     2821 gccgagctcg gcgaggatgc gttcgggggc ttcgaccacc gacgcgaacg gtccgctggt
     2881 ctcggtggcg aagcaggtgg acgctgcaac ctcggtccca cgacaacgaa cgtcgccatc
     2941 tggggtagag agcagtgtga agcctccgtc cggggacgct ccgtccatgc gccatcggtc
     3001 gggcgggtcc cacgcgagtc gcatctcgcc gacgccggag cagatcctca agccggtctg
     3061 gacatcgacc tcgacgagct ggcgcagaca ctgaggcgga gatgttgcct ctccagggtc
     3121 gcgttcggtc gtgcggtacg tgatcgtggc cgggctcgcg aaccacaggt cttccaacgt
     3181 caggagtcgc gtgcgcggct cttcgaccgg cgcatctgca ccgtggtcgc aggctgaaac
     3241 gaccgtcgcc gcgacgacga cgagcacgag cacgcttcga gatcgcagca agcggacacg
     3301 gcgaagccat agcatccgac catcatggcc gagacctcgc tcgaccatgc gcaggtcacg
     3361 cgctcgggta gccttcggcc atggcgccga gcgggctgct gaccgacctc tacgagctca
     3421 acatggccgc cagctacctc cagcggggga tg
//
LOCUS       DASUYD010000152        13255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_80504, whole genome shotgun sequence.
ACCESSION   DASUYD010000152 DASUYD010000000
VERSION     DASUYD010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13255
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80504"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..905
                     /locus_tag="VF195_08450"
     CDS             219..905
                     /locus_tag="VF195_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014923168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX6580888.1"
                     /translation="MTRGTGTAKKAPLSKERIVQAAVALADAGGFDSLSMRSLAQDLS
                     TAPMSLYRHFANKEGLLDGMIDVVFGQMYSPAIRGAWKTELRRRGMAAREALRRHPWA
                     VGLMETRMNPGPASAEHHNAVMGCLREGGFPFRDAVHAYNVLDAYMYGFALQEKTIPF
                     VTAEQSAQMAEITVGERGSEYPYLAEVVHEFATSGGYDYAEEFEFGLDLVLDGLERLK
                     RTLRSGRTSG"
     gene            complement(988..2130)
                     /locus_tag="VF195_08455"
     CDS             complement(988..2130)
                     /locus_tag="VF195_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015309457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6580889.1"
                     /translation="MSGDARILVVDDVAPNVRLLEAVLVPRGYEVVSATDGASALELV
                     ASANPDLVMLDVVMPQPDGYAVCRAIREREETAMLPVIMITASTSERTEAIRAGADDL
                     IAKPFDQDELLARVRSLLRIKRYHDTITSQASELTELNRTLEERVQTQVVELERIRKL
                     RRFLSPQLVDAIVSSGDDTILRSHRRKVAMFFADLRGWTSFVDAVEPEELMQVLGEFH
                     RVIGGLVRRFDATVGFLEGDGVQLFFNDPHEAPDAPLRAIRLGCALREEMAELTPAWR
                     KRGYELDFGAGIALGYATCGEVGFEGRSDYAAIGAVTNLAARLADEATAGQVLITQRL
                     HAEVEDDVDVEPIGELTLKGFRTPIPALNVIAVHEPAENVSAVQGY"
     gene            complement(2127..2507)
                     /locus_tag="VF195_08460"
     CDS             complement(2127..2507)
                     /locus_tag="VF195_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6580890.1"
                     /translation="MTGERILVVEDNEKNMKLFRDVLVATGYRTLEATTGGEAVDMAS
                     EHTPDLVLMDIQLPDLDGVEALHRLRADERTATIPVLALTAQAMRGDRERFLAEGFDG
                     YVSKPVNVREFLGTVRQHCEGRLG"
     gene            complement(2504..4138)
                     /locus_tag="VF195_08465"
     CDS             complement(2504..4138)
                     /locus_tag="VF195_08465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX6580891.1"
                     /translation="MAEELGTRVAAERRQRIDPLVRAVARLPVTVRTKLLIAFVGTSL
                     LLVAVGLLGQVVLGQSNDRVASVRALQERAVEYSRLKAAAESLRDVLAQNVGDDFNVI
                     WPDAAPPGIRGTYSLAVDLSAVDAAIGVASKTTPDQLGFTPPPGDQLVLQEIEGTAQR
                     LRRLIQQRLIPLYAGPTIAIDDEATIEQMLPIRARAEDYASDLATGATGLANGTRHEI
                     QDLIARNKSSFASSRALFIGVAAGAIVLALLLGFVLSWSVIGPIQNIDTRLAAIASGD
                     FSGHVEVGNRDELGALGANVNRMNDELRRLYTELEAASRHKSEFLANMSHELRTPLNA
                     IIGFSQVLRDEMVGPVNEKQAEYLDDISSSGNHLLSLINDVLDLSKVEAGQVELQMHP
                     FSLREALERGVVMVRERATEEGVRVAFAADPEVDVVEGDERRIKQVIFNLLSNAVKFT
                     PAGGEIDVSATRVNGEVRVSVADTGPGVAPEDRERIFEEFQQSETGVGHREGTGLGLA
                     LSKRFVELHGGRIWLESELGHGSTFTFALPAESSTS"
     gene            complement(4143..4649)
                     /locus_tag="VF195_08470"
     CDS             complement(4143..4649)
                     /locus_tag="VF195_08470"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6580892.1"
                     /translation="MRRLVIGGGLGVFVLSVVLVACGGSTTGSSGDQARRFQDMWEID
                     QIEKRFHEAQTKKDIDLMMSLYAPDATMTVGPGVTGSGVDEIRRFWLEDSAPFAAENE
                     WISDHPAYKLEITVDGDRGTLHFECHYVDAETREVVSATAGEFDVARIAGKWLITNFV
                     GATTVLEV"
     gene            complement(4808..5179)
                     /locus_tag="VF195_08475"
     CDS             complement(4808..5179)
                     /locus_tag="VF195_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6580893.1"
                     /translation="MTSVLVVDDNDRNRKLALEVLSAAGFRTFGAATAVQGIALAREH
                     VPDVILMDLRLPDTDGVEATRKLAAHERTALIPVVAMSASPLEGSEDWLEEAGFAGWL
                     EKPIHVSTFPEQVRRYSLKTE"
     gene            5197..5862
                     /locus_tag="VF195_08480"
     CDS             5197..5862
                     /locus_tag="VF195_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6580894.1"
                     /translation="MPIRLVIADDHLLVREGVRRLLETDPDIEVAAVCGDLDSLLAAV
                     EAEHPDVVVTDIRMPPDNTDEGIQAADRLRTTDPDVGVVVLSQYATPSYALALLEGGS
                     AGRSYLLKERVQEPDQLVSAIRAVAAGGSVIDPKVVEALVAEDVRGERSPLKSLTERE
                     REVLREMAAGKSNLAIGESLFLAERSVEKVIHSIFLKLGLTWEPSVNKRVKAVILYLA
                     ESD"
     gene            complement(5865..6473)
                     /locus_tag="VF195_08485"
     CDS             complement(5865..6473)
                     /locus_tag="VF195_08485"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6580895.1"
                     /translation="MRREVDELRASRKRLALAADGERRGIERALHEGVQQDLVGLAAN
                     LEVAAGSVDSDPAAAKALLEELQREARRAITEMQELASRIFPALLEAAGLVPELRAAA
                     SRAGVPVELAADVHVSVPPELAGAVYFCALDAFERAPDGTPVSVSVRGEEDELAFEIE
                     VDGDLGSERRAPHDRVEGLGGQVMITAASGRTTVAGSMPIPR"
     gene            6715..7293
                     /locus_tag="VF195_08490"
     CDS             6715..7293
                     /locus_tag="VF195_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580896.1"
                     /translation="MNRRIVLSIAVGLVALASAAYGKTFGAWGTPVNAESVAGASSDL
                     NTPYNDGCPIQSPDGLSLYIASNRPGGLGGQDIWVAHRDEPHGPYGAPVNLGAPVNSA
                     VDDFCPSPTGGRRLFFVSARSGGCGGPDIYVTRLDLDGWRAPRNLGCTINSAAGEASP
                     SLFRDDTATRSSISRAHVREASRREAPTPTST"
     gene            7290..7643
                     /locus_tag="VF195_08495"
     CDS             7290..7643
                     /locus_tag="VF195_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580897.1"
                     /translation="MSVDFGPAQLAPGLNTANEDARPNVRKDGLEVVFDSTRAGGLSA
                     LPDIWTASRSNDAAAWSSPVHLDAPINSEASETRASLSWEGVTMVFGSTRPGSEPAPD
                     GLTISNDVYVVTRST"
     gene            7985..8485
                     /locus_tag="VF195_08500"
     CDS             7985..8485
                     /locus_tag="VF195_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6580898.1"
                     /translation="MKKSVFVLTATLLTLGAIGIGMLPLAAQEPPLPIATEVLSPRSV
                     FPDDIDMKVKINTGGETEVVKVVDPSRTVTARFTIQPGAHFPWHSHAGPVIVNVVQGT
                     LVFVEADCGETAYPAETAFVDAGHGHVHSAYNPTDGETVIVATFFESPATGSLLIPAD
                     TPACAA"
     gene            complement(8792..11104)
                     /locus_tag="VF195_08505"
     CDS             complement(8792..11104)
                     /locus_tag="VF195_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6580899.1"
                     /translation="MFAKLGRWCFRNRGKVALLWLAALIVFGGISSMVGSAYSTEFAL
                     PDVESRRGFDILDEHFADSGAGGEGGTIVFRAEQGVTDPEVQRAMTAFFESAGQIEGI
                     TIISPYTPEGAQQIASQGPEAGLIAYAQIGLPRDITLEEATAIGAELKELMPEIAGVQ
                     IEIGGAILAEFEVPSSELLGLAFAMIVLILAFGSVLAMGLPVGIALAGIGVGTILAGF
                     LSHVLSIPDFATTIAVMLGLGVGIDYALFIVTRFRENLHKGASIEDATVVALDTAGRA
                     VAFAGTTVVISLLGMVLMGLAFVTGLAAASATVVAVTAVASLTLLPALLGFAGERIEL
                     TRWRGLIAALLVAVGLVGVALTIQPLALVGISLAAAVLLASFAFTPLRREVPRRKPRP
                     LRETIPYRWSRFIQHRPWVAAISGASILIVLAIPFFSQRLAFSDEGNYPEETTTRKAY
                     DLLAAGFGPGFNGPLLLSTEIPEGTDPASLETVTEALAGDPGTAFVTPAVPNDPEEPT
                     AALWRLFPTTAPQDESTTDLVYRLRDEVLPNATQGTELDIAVSGAVAAAVDFSEYLTA
                     RLPLFIGVVLGLSFLLLMLVFRSLLVPLKAVVMNLLSIGAAYGVVTAIFQWGWLGDVI
                     NIESAPIEPFVPMMMFAILFGLSMDYEVFLLSRIREEYVRTGDSRLSVADGLASTARV
                     ITAAAAIMVVVFGSFIGESDRIVKLFGVGLSIAVLLDATIVRMLLVPATMELLGDANW
                     WLPRWLDRAMPRVAVEGHPLEVRPPELETV"
     gene            complement(11942..12511)
                     /locus_tag="VF195_08510"
     CDS             complement(11942..12511)
                     /locus_tag="VF195_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6580900.1"
                     /translation="MGFVSTVASFGIGYAAGTISGRQGIEQLSGRVRQALSSRGRSGD
                     ATSTLDVREIRQVMTTPPDTVRKTASLQEAARLMKTKDIGDVLVEDEQGRLAGIITDR
                     DVAIRATAEGADPKTTKVEKAYTQDVTALAPTDTVHDAVRLMRSKDVRRLPVVEAGKA
                     VGIVSLGDISVQTAPRSLLADISTAAPDR"
     gene            complement(13022..13093)
                     /locus_tag="VF195_08515"
     tRNA            complement(13022..13093)
                     /locus_tag="VF195_08515"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13059..13061),aa:Val,seq:gac)
     gene            complement(13117..13187)
                     /locus_tag="VF195_08520"
     tRNA            complement(13117..13187)
                     /locus_tag="VF195_08520"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13153..13155),aa:Cys,seq:gca)
BASE COUNT         2229 a         4524 c         4312 g         2190 t
ORIGIN      
        1 accccccagg ccactcacga agagcaatct ccccgcgatc cccgtgatga acgtgagcag
       61 gaacaggatg cctgccgtcc ttgcgatctt ctgcgtatcc atcggtctcc ctctgtccgc
      121 ctcgatcgga ggctctccca gtgcgtacac cgtacgcaac gcgtgctaac ctatgcgtac
      181 ggcgtacgct ctgtcaaccg gggatccgag gaggtcgagt gactcgagga acgggcaccg
      241 ccaagaaggc tcctctgagc aaggagcgga tcgtccaggc agccgtggct ctcgcggacg
      301 cgggcggctt cgactccctc agcatgcgca gcctcgcgca ggatctgagc accgcgccga
      361 tgtccctcta tcggcacttc gcgaacaagg aggggctcct cgacggcatg atcgacgtcg
      421 tcttcggcca gatgtactcc cccgccatcc gaggtgcgtg gaagacggag ctgcgcaggc
      481 gcgggatggc cgctcgtgag gctctgcggc gccatccctg ggcggtcggc ctcatggaga
      541 cgcgcatgaa ccccggcccg gcgagcgccg agcaccataa cgccgtgatg gggtgcctgc
      601 gggaaggggg gttccccttc cgagacgcgg tccatgcgta caacgtgctg gacgcctaca
      661 tgtacggctt cgcgctgcag gagaagacga tcccgttcgt gacggccgag cagtccgcgc
      721 agatggccga gatcaccgtg ggcgaacgag ggtccgagta cccctacctc gcggaggtcg
      781 tgcatgagtt cgcaacgtcg ggcgggtacg actacgccga ggagttcgag ttcggcctcg
      841 acctcgtcct cgacgggctc gaacgcctca agcgaacact ccgttccgga cggacatcag
      901 gctgagcagc catcatcgag tccttccgaa acgatcagac gatcggttcg atccccatca
      961 ccaggaagag gtgggcggag tcacgcctca gtagccctgc acggcggaca cgttctcggc
     1021 cggctcgtga acggcgatga cgttgagtgc cgggatcggc gtacggaacc ccttgagcgt
     1081 gagctcaccg atgggctcaa cgtcgacgtc gtcctcgacc tccgcgtgga gtcgctgggt
     1141 gatcaggacc tggccggcag tggcttcgtc ggcgagccgc gctgcgaggt tggtcaccgc
     1201 gccgatcgcc gcgtagtcgg agcggccctc gaatccgacc tcgccgcacg tcgcgtagcc
     1261 gagcgcgatg ccggcaccga agtccagctc gtagccgcgc ttgcgccacg ccggcgtcag
     1321 ttccgccatc tcctctcgga gcgcgcatcc cagccggatc gctcggagcg gagcgtcagg
     1381 ggcctcgtgg gggtcgttga agaagagctg gaccccgtcg ccctcgagga accccacggt
     1441 cgcatcgaag cgtcgcacga gaccgccgat cacacggtgg aactccccga gcacctgcat
     1501 cagctcctcg ggctcgaccg cgtcgacgaa gctggtccat ccgcggaggt cggcgaagaa
     1561 catcgcgacc ttgcgacgat ggctgcggag gatcgtgtcg tcacccgacg agacgatcgc
     1621 gtctacgagc tgcggagaga ggaaccgccg gagcttccgg atccgctcga gctccacgac
     1681 ctgagtctgc acccgctcct cgagcgttcg attcagctcg gtcagttccg aggcctgcga
     1741 cgtgatcgtg tcgtggtagc gcttgatacg caggagcgac cggacccgcg cgagcaactc
     1801 gtcctggtcg aacggcttgg cgatcagatc gtccgcaccc gcccggatcg cctcggtcct
     1861 ctccgaggtg ctggccgtga tcatgatcac cggcagcatg gcggtctcct cgcgctcccg
     1921 tatcgcgcga cacaccgcgt acccgtccgg ctgcggcatc acgacgtcga gcatcacaag
     1981 gtccgggttg gccgacgcga cgagctcaag cgcgctcgcg ccatcggtcg cggatacgac
     2041 ctcgtagccg cgcggcacga gcacggcttc gagcagccgc acgttcggag cgacgtcatc
     2101 cacgacgagg atccgggcgt cgccgctcat ccgagccgtc cctcgcagtg ctgccgaacg
     2161 gtcccgagga actcccgaac gttcacgggc ttcgacacgt agccgtcgaa gccttccgcc
     2221 aggaaccgct cgcgatcgcc tcgcatcgct tgggcggtca gtgcaaggac ggggatcgtc
     2281 gccgtccgct cgtccgcccg caatcgatgg agcgcttcca cgccgtcgag gtccgggagc
     2341 tggatatcca tcaagacgag gtccggcgtg tgctcggatg ccatatcgac cgcctcgccg
     2401 ccggtcgtcg cctcgagcgt tcgatatccc gttgcgacca ggacatcgcg gaacagcttc
     2461 atgttcttct cgttgtcctc gaccacgagg atccgttcgc ccgtcatgag gtgctcgact
     2521 ccgcgggcaa cgcgaacgtg aaggtgctcc catgaccgag ctcgctctcg agccagatcc
     2581 gtccgccgtg gagctccacg aaccgcttcg agagcgcgag gcccaggccg gttccctcgc
     2641 gatgcccgac ccccgtctcg ctctgctgga actcctcgaa gatccgttct ctgtcctcgg
     2701 gagcgacccc cgggccggta tcggcgacgg agaccctgac ctcgccgttg acccgcgtcg
     2761 cgctcacgtc gatctcccca ccggcgggcg tgaacttcac cgcgttcgaa agcagattga
     2821 agatgacctg cttgatccgt cgctcgtcgc cctcgaccac gtcgacctcc ggatcggcgg
     2881 cgaacgcgac gcggacccct tcctccgtcg cacgctcgcg gaccatcacc acaccacgct
     2941 cgagcgcctc tcgtagagag aacgggtgca tctggagctc gacctgtccg gcctcgactt
     3001 tggagaggtc gaggacgtcg ttgatcagcg agaggaggtg attgcccgaa gagctgatgt
     3061 cgtcgaggta ctccgcctgc ttctcgttca cgggcccgac catctcgtcg cgcagcacct
     3121 gcgagaagcc gatgatcgcg ttcagcggtg tccgaagctc atgggacatg ttggccagga
     3181 actccgactt gtgccgactc gccgcctcga gctccgtgta gagacgccgc agctcgtcgt
     3241 tcatccggtt cacgttggca cccaacgcgc cgagctcgtc gcggttcccg acctccacgt
     3301 ggcccgagaa atcgccggat gcgatcgcgg cgaggcgcgt gtcgatgttc tggatcggcc
     3361 cgatcaccga ccaggagagt acgaacccaa gcagcagcgc gaggacgatc gcccccgccg
     3421 cgacgccgat gaacaacgcc cgggagctcg cgaacgagct cttgttccgg gcgatcagat
     3481 cctggatctc atgtcgcgtc ccgttggcca gtcccgtggc tccggtagcg aggtcgctcg
     3541 cgtagtcctc cgctcgcgcg cggatcggca gcatctgctc gatcgtcgcc tcgtcgtcga
     3601 tcgcgatcgt tgggcccgcg tagagcggga tgagcctctg ctggatgagc ctccgaaggc
     3661 gttgggcggt gccctcgatc tcttgcagga ccaactgatc acccggaggc ggcgtgaaac
     3721 cgagctgatc cggagtcgtc ttcgaggcga ccccgatggc cgcgtcgacg gcagaaaggt
     3781 ctaccgcgag agaatacgtc ccgcggatcc cgggaggcgc tgcgtccggc cagatcacat
     3841 tgaagtcgtc gcccacgttc tgcgccagga catcacggag ggactcggct gcggccttga
     3901 gcctgctgta ctcgacggcc cgctcttgga gcgccctgac gctcgcgaca cgatcattcg
     3961 actgcccgag cacgacctgc cccagcagac cgaccgcgac caagagcagt gacgtgccga
     4021 cgaacgcgat cagcagcttc gtccgaaccg tgaccggcaa gcgggcgacg gcccgaacga
     4081 gtggatcgat ccgttgccgc cgctcggcgg cgacccgcgt ccccagctct tcggccaaga
     4141 cctcagactt ccaagaccgt cgtcgcaccg acgaagttcg tgatcaacca cttcccggcg
     4201 atccgggcga cgtcgaactc gccggccgtg gccgacacga cctcgcgagt ttcggcgtcg
     4261 acgtagtggc actcgaagtg cagcgtgccc cggtcgccgt cgaccgtgat ctcgagcttg
     4321 tacgcggggt gatccgagat ccactcgttc tcggccgcga acggcgccga atcctccagc
     4381 cagaatcgcc tgatctcatc cacgccggat cccgtcaccc cgggacccac cgtcatcgtg
     4441 gcgtcgggcg cgtacaggct catcatgaga tcgatgtcct tcttcgtctg ggcctcgtgg
     4501 aaccgcttct cgatctgatc gatctcccac atgtcctgga atcgtcgggc ctggtccccg
     4561 gacgagccgg tggtcgagcc gccgcaggcg acgagcacga ccgagagaac aaagactcca
     4621 agtccgccac cgatcacgag cctccgcatg tccgtccccc ttctgctcag gccatccggc
     4681 cgtcgatgct cggcgatgtt cacaccgccc agccgatccg tccagaggct agggacaaca
     4741 atggtctcgg cccacctggt caaccgcctt tgatcggggt gccagcacgg tactggcgtc
     4801 gacctcgtca ctccgtcttc aggctgtagc ggcggacctg ctcggggaac gtgctcacgt
     4861 ggatcggctt ctcgagccat ccggcgaagc ccgcctcctc cagccagtct tcgcttcctt
     4921 cgagcggcga tgcgctcatc gcgaccaccg ggatgagcgc cgttcgctcg tgtgctgcga
     4981 gcttccgcgt ggcttctacg ccgtccgtgt cggggagccg gaggtccatc aggatcacgt
     5041 ccggcacgtg ttcccgcgcg agcgcgatcc cctgcacggc tgtcgccgca ccgaacgtcc
     5101 ggaaccccgc cgcactcagg acctcgaggg cgagcttccg gttccggtcg ttgtcgtcga
     5161 cgacgaggac gctggtcacg ggcgtatcct cgcgccatgc cgatccgact cgtgatcgcc
     5221 gacgatcatc tcctcgtgcg cgagggcgtg cgccggctgc tcgagaccga tcccgacatc
     5281 gaggtggcgg cggtctgcgg cgatctcgac tccctgctgg ccgcggtgga ggccgagcat
     5341 ccggacgtgg tcgtcaccga catccgcatg ccgcccgaca acacggatga ggggatccag
     5401 gcggcggatc gactccgtac cacggatccc gacgtgggcg tcgtcgtgct gagccagtac
     5461 gcgacgccga gctatgccct cgcgctcttg gagggcggca gcgccggccg ctcctatctc
     5521 ctgaaggagc gcgtccagga gcctgaccag ctcgtgtcgg cgatccgcgc ggtcgcggcc
     5581 ggtggctcgg tgatcgaccc gaaggtcgtc gaggcactgg tcgcggagga cgtgcgcggc
     5641 gagaggtcgc cgctcaagag cctgaccgaa cgcgaacgcg aagtgctgcg cgagatggct
     5701 gccggcaaga gcaacctggc gatcggtgag tccctcttcc tcgccgagcg gtcggtcgag
     5761 aaggtgatcc attcgatctt cctcaagctc gggctcacct gggagccgtc ggtcaacaag
     5821 cgggtcaagg ccgtgatcct ctaccttgcg gagagcgact agcgtcatcg cgggatcggc
     5881 atggatccgg ccacggtggt ccggccgctc gccgccgtga tcatcacctg gccgcccagc
     5941 ccctcgacac gatcatgcgg ggcgcggcgt tccgatccca ggtcgccgtc cacctcgatc
     6001 tcgaacgcga gctcgtcctc ctcgcctcgg acgctcacac tcaccggagt gccgtcgggg
     6061 gcgcgctcga acgcgtcgag cgcgcagaag tagacggcgc cggcgagctc cggcggcacg
     6121 gagacgtgca cgtcggcggc gagctccacc ggcacgccgg cgcggctcgc cgccgcgcgg
     6181 agctccggga ccaaccctgc ggcctcgagc agcgcgggga agatccggct ggccagctcc
     6241 tgcatctccg tgatcgcccg ccgcgcttcg cgctgcagct cctccaggag tgccttcgcc
     6301 gcggcgggat ccgagtccac cgatcccgcg gcgacctcga ggttggcggc gagccctacg
     6361 agatcctgtt gcacgccctc gtgcagcgct cgctcgatac cgcgacgttc gccatcggcg
     6421 gccagggcta gccgtttccg agaggctcgg agctcatcga cctcccggcg caacccatcg
     6481 agggtcggct gatcgtcggt ctgatcgatc tccagcggat cccctcctgc gcctggatac
     6541 ccggcagttg tccgtcatcc ctcggacggc gtcaagcggc cagcaccgta tcggggcgga
     6601 tggcagcatc tcggtcttcg ccaggccgcc accgtagagc cggcgggaac gacctcctac
     6661 cgtcgcgatg aggtgatcgg gacctgaccc gatccggatc ggaaggggga ggagatgaac
     6721 cgtcgcatcg tgctcagcat cgccgtgggg ctcgtggcgc tcgcgtcggc cgcctacggg
     6781 aagacgttcg gagcgtgggg aacgccggtg aacgccgagt cggttgccgg cgcgagctcc
     6841 gacctgaaca ccccctacaa cgacggctgc ccgatccagt caccggacgg cttgagcttg
     6901 tacatcgcct cgaaccgtcc cggcggtctg ggcggacagg acatctgggt ggcccatcgc
     6961 gatgagccac acggtccgta tggcgcgccc gtgaacctgg gcgctccggt caacagcgcc
     7021 gtcgacgact tctgcccttc gccgaccgga gggcggcggc tcttcttcgt gagcgcgcga
     7081 tccggcggct gcggcggtcc cgacatctac gtcacacgct tggacctcga cggatggcgc
     7141 gccccccgca acctcggctg cacgatcaac agcgcggccg gggaggcgag cccgtccctc
     7201 ttccgcgacg acacggccac tcggtcctct atttctcgag cacacgtccg ggaggcttcg
     7261 aggcgggagg caccgactcc gacatctact tgagcgtcga cttcggccca gcgcagctgg
     7321 cgccagggct gaacacggcg aacgaggacg cgcgtccgaa cgtccggaag gacgggctgg
     7381 aggtcgtgtt cgactccacg cgagcgggcg gcctcagcgc cctgcccgac atctggacgg
     7441 catcgcgttc gaacgatgcc gccgcgtggt cgtcgccggt ccacctggat gccccgatca
     7501 acagcgaggc cagcgagacc cgagcgtccc tctcctggga aggggtcacg atggtcttcg
     7561 gctcgacccg cccagggagc gaaccggcac ccgacgggct gacgatctct aacgacgtgt
     7621 acgtcgtcac gcgatcgacc tgagccgccg cctcgatgct ccgaggagcc cggtcgccgc
     7681 aggcgaccgg gcttcggccc agcgtgccgc tcgaaggtgg cgatctgacg cgggcttgag
     7741 atgaaaggtc gacgctgaac cgtcggcggg atccgtgtcc gtcatcgctc ggacgacgtc
     7801 aagcggccca gcaccgtatc ggaccggaag gcagcatccc cccacatcgc ccggccgcca
     7861 ccgtggagtc gccgggaacg acctcctagc gtcgggatgg acgagcgctc atcgggaatc
     7921 gagagagagg aggaggggtg atgaggctca gacaggtgat gcgaggaacg ggaggagacc
     7981 gcagatgaag aagtcagtgt tcgtgttgac agcaactctg ctcaccctgg gtgcgatcgg
     8041 catcgggatg ttgccgctcg ccgcccagga gccgccgttg ccgatcgcga ccgaggtcct
     8101 gtcgccgcga agcgtgttcc cggacgacat cgacatgaag gtcaagatca acaccggtgg
     8161 cgagaccgag gtcgtgaagg tggtcgatcc ctcgcggacg gtgacggccc ggttcacgat
     8221 ccagccggga gcgcatttcc cgtggcacag ccacgcagga cccgtgatcg tcaacgtggt
     8281 ccagggcacg ctcgtgttcg tcgaggccga ttgcggggag acggcatatc cagcggagac
     8341 cgccttcgtc gatgccggcc acgggcacgt ccattccgcc tacaacccga ccgatggaga
     8401 gaccgtgatc gtggcgacgt tcttcgaatc tccggccacg ggatcgctgc tgatcccggc
     8461 ggacacgccg gcgtgcgccg cgtaggaata ccgagggtct cacgaaccac gtctcggatg
     8521 gtggggttgc ttcggcagcc ccaccttccg cgatgaggcg cgtaggtcgg cgcgagcgcg
     8581 ttccgtcccg tcgtcgggcc gctcggcaac atggaccggt gcactccgat ccttcgagtt
     8641 cgaccgcgga cgtcgttcga gtgacccgcc tcgatcagtc ctggatgagg cgatccggcg
     8701 acacgtctcg ggacgaagcc tcgacgcgcc ttcgccacct cgcggagccc acaggtgatc
     8761 agggctcgat gccgagaccc tcatcgcgaa ctcacacggt ctcgagctcc ggcggtcgca
     8821 cctcgagggg gtggccctcg acggcgaccc gcggcatcgc ccggtcgagc cacctcggga
     8881 gccaccagtt ggcgtcgccg agaagctcca tcgtggcggg cacgagcagc atccggacga
     8941 tcgtcgcatc caggagcacg gcgatggaga gacccacgcc gaagagcttg acgatgcggt
     9001 cgctctctcc gatgaagctg ccgaacacca ccaccatgat ggcggctgcc gcggtgatca
     9061 cccgtgccgt ggacgccaga ccatcggcca cggagagcct gctgtcgccg gtacgcacgt
     9121 attcctctct gatacgggag agcaggaaca cctcgtagtc catggacaac ccgaagagga
     9181 tggcgaacat catcatcggc acgaacggct ctatgggcgc ggactcgatg ttgatcacgt
     9241 ccccgagcca gccccattgg aagatcgcgg tgacgacacc gtaagccgct ccgatcgaga
     9301 gcaggttcat caccaccgcc ttgagcggaa ccagcaggga ccggaacacg agcatcagca
     9361 gcaggaagga aagaccgagc acgacaccga tgaagagcgg cagacgcgcg gtgaggtact
     9421 cggagaagtc gaccgcggcc gccaccgcgc ccgagacggc gatgtccagc tctgttccct
     9481 gtgtcgcgtt cggcaggacc tcgtcgcgta ggcgatacac caggtcggtg gtcgattcgt
     9541 cctgcggggc ggttgtcggg aagagccgcc acaacgctgc cgtgggctct tcgggatcgt
     9601 tcgggaccgc aggcgtgacg aaggctgtcc cggggtcgcc tgccagggct tcggtaaccg
     9661 tttcgaggct cgcgggatcc gtcccctcgg ggatctcggt gctgagcagc agcggcccgt
     9721 tgaagcccgg tccgaagccg gcggccaaca gatcgtatgc cttgcgggtg gtcgtctcct
     9781 ccgggtagtt gccctcgtcg gagaaggcga gccgctgact gaagaagggg atcgcgagca
     9841 cgatgaggat cgatgcgccg ctgatcgccg cgacccacgg gcggtgctgg atgaagcggc
     9901 tccatcggta ggggatcgtc tcgcgcaatg gcctcggctt gcggcgaggg acctcacgcc
     9961 gaagcggtgt gaacgcgaag ctcgccagga gcacggcggc ggccagcgat atcccgacga
    10021 gagccagcgg ctggatcgtg agggcgacgc cgacgagacc caccgcgacc aagagagcgg
    10081 cgatgagtcc ccgccatcgg gtcaattcga tccgttcacc ggcgaagccg aggagagcgg
    10141 ggagcagggt caacgaggcg accgcggtga cggcgacgac ggtggccgag gcggcggcga
    10201 gccccgtcac gaacgccagg cccatgagga ccatgccgag cagtgagatg accacggtgg
    10261 tgcccgcgaa cgcgacggcc cggccggcgg tatcgagggc aacgacggtt gcgtcctcga
    10321 tcgaggcccc cttgtgcaga ttctcccgga atcgggtcac gatgaagagc gcgtagtcga
    10381 tgccgacacc gaggccgagc atcacggcga tcgtcgtggc gaagtcgggg atcgacagaa
    10441 cgtggctcag gaatccggcg aggatcgttc ccaccccgat gccggcgagg gcgataccga
    10501 cggggaggcc catggcgagc accgatccga acgcaaggat gagcacgatc atggcgaagg
    10561 ccaggcccag cagctccgag ctcggtactt cgaactccgc gaggatcgct cccccgatct
    10621 cgatctggac acctgcgatc tccggcatca gctccttcag ctctgcgccg atggcggtcg
    10681 cctcctccag cgtgatgtca cgagggagtc cgatctgggc ataggcgatg agcccggcct
    10741 ccggaccctg tgaggcgatc tgctgcgcgc cttctggggt gtacgggctg atgatcgtga
    10801 tcccctcgat ctgacccgcg ctctcgaaga aggcggtcat cgctcgctgc acctcggggt
    10861 cggtgacgcc ctgctcggcg cggaacacga tggttccgcc ctcgccgccc gcgcccgagt
    10921 cggcgaagtg ctcatcgagg atgtcgaacc cgcggcggct ctccacatcg ggaagtgcga
    10981 actccgtgct gtacgcgctg ccgaccatgc tgctgatgcc gccgaacacg atcagtgcgg
    11041 ccagccacaa caaggcgacc ttgcctcggt tacgaaagca ccaacgcccg agcttggcga
    11101 acatgactgc tcctctgtga ccgccaggag gctagaagga tagtcgacca catccgcctc
    11161 ggagatggtc ctcggccggt ctcacctgaa cggatcggcc aatgtcgcgc tgagttggcc
    11221 ggccgtggtc cgcccgtctg ttggtgggcg ccagagtgcg cggaggtgag gggatatgaa
    11281 cccctggcct cttccgtgtc aagaagcccc acgtagggct ctaacctgca tcttcgcagc
    11341 tgaggcccca tttcagaacg tctcaggagt tccgtctggt accgcttggt tcggtcggct
    11401 cctggacctg gtgttgacca aacggggccc atgcttcgct acccaagctg ggcgccggga
    11461 ctgtgtcgac cctctcgcat ggtcggcctc caaggctacg gccgttccac tgctcatcga
    11521 ggatccgggc ggcgggtgat tcgaagtcag cgacgccgtc cgctcacacc gggctcgtcg
    11581 tctctcgcgg agtctctcat ggggggtttg ccgcccagag ctccgtgtgg ccggtctccc
    11641 acgccttgag tcgatcgttg aagaacgcgg tgtcgtcgat caccaccccg acgagcatcc
    11701 gatagaactc ctcggtgtcg atccggtgtg aacgttcaac cttcccggtc gacctcgggg
    11761 tggccggctt gacgtagacg ttggccagcc cccggcatcc aggacgtggt agtggaactg
    11821 cgacccgaac tccgttccct tgtcggtctg gaccacgtcg acacggaacg ggagcttctc
    11881 gaaagacgta gtcgatgaac cggatcgccg tctgttggtt gagccggttc gatgcagaga
    11941 ctcagcggtc aggagctgcc gtgctgatat ccgcgagcag cgagcggggc gcggtctgca
    12001 ccgagatatc gccgagcgag acgatcccaa cagccttccc ggcctccacc accggcaggc
    12061 gccgcacgtc cttggatcgc atcagccgca cggcatcgtg caccgtatcc gtaggggcga
    12121 gggcggtgac gtcttgcgtg taggccttct ccaccttcgt cgtcttggga tccgctccct
    12181 cggccgttgc gcggatcgcg acgtcccggt cggtgatgat gccggccagc cggccctgct
    12241 catcctcgac cagcacgtcg ccgatgtcct tcgtcttcat caagcgagca gcctcctgca
    12301 gactggcagt cttccggacc gtatcgggag gcgtcgtcat cacctggcgg atctcccgaa
    12361 cgtccaacgt ggaagtcgcg tccccagagc gaccacgtga cgacaatgct tgccgtacac
    12421 gaccagacag ctgttcgatg ccctggcggc ccgaaatcgt ccccgccgcg tatccgatgc
    12481 cgaagctcgc aaccgtgctg acgaacccca tctcgcttcc tccccagatc tccttgatcg
    12541 ctccgtaccc acggcacgtc ctttcaacgc acggactgga gaaccgcccc ggctcgtggg
    12601 gtcatcgaga gccaatggct ccaatcaccg gataaccgcg gcgatacgag atcaccagct
    12661 agatccggtc cacgggctgc catcccgtag ctatggccct tgtctttgac tcgcggtgga
    12721 ccttctcgcc gcggaggaag tcggagacat tgggagaagt agcgcaaacg gcgttcgtct
    12781 tctgcggtcg aacatcaggc tgggggttct gcagatccct gctggcgatc tgggttgcag
    12841 gccagaacgc ggctgacaca agaccgctcc ggcattctcg accccttcga aaaggccctg
    12901 actacgcgct cgcgcatccg ccaggcatcg tggtgcctgt ttggttcctt ttcgggttcg
    12961 gcgctgaatc ttatggcgac ctccacgagc gaataggccc cgtgaccagg cctttctctt
    13021 gtgggcgcta gaggatttga acctctgacc tcttccgtgt caaggaagcg ctctccccct
    13081 gagctaagcg cccgagccct catcgtagcg gcctcgaggc ggggaccgga atcgaaccgg
    13141 tgtgcgaggc tttgcaggcc tctgcctaac cactcggcca ccccgccgga cgaggggcca
    13201 gtgtagccgc ggctcgcggc aggcttgccc ctcgaagggt gcagggtaca gaaac
//
LOCUS       DASUYD010000153         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_303215, whole genome shotgun sequence.
ACCESSION   DASUYD010000153 DASUYD010000000
VERSION     DASUYD010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303215"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF195_08525"
     CDS             complement(<1..912)
                     /locus_tag="VF195_08525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX6580901.1"
                     /translation="MPITEVEKIWMDGELVDWGDAKIHVLSHAVHYGSGVFEGIRAYE
                     TARGPAVWHLDEHLKRLFRSAKLYHMDIPYSIEAITDATKEVIRANGLNACYVRPLVV
                     RGYGEMGVNPLNAPVNVIIAVWPWGAYLGEDALENGVRIKISSWRRNSQNALPSSAKA
                     TGQYINGVLAKIEGLKAGYDEAVMLNEQGFITDGSGENLFIVRDGKLTTPPIQAGCLD
                     GITRGTVLTIARDLGYDVTEENLVRTDLYNADECFFSGTAAEITPIREVDDRTVGEGH
                     RGPVTKELQGAFFAATKGETERYASWLT"
     gene            complement(1021..1800)
                     /locus_tag="VF195_08530"
     CDS             complement(1021..1800)
                     /locus_tag="VF195_08530"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6580902.1"
                     /translation="MSESIRFDSAADHYDRTRAISDETMARTIALLASELATRGRVLE
                     VGVGTGLLALRLHEAGFSLAGLDLSAPMLGKLVEKAGGEPVFPLVLGDATRMPFGDGT
                     FDGAYLRWVLHLIPDWRSALAEMVRVVHPGGLLLVCLGAFDQVSSAIRARFTEVTGLS
                     TDPVGLMWGDHEALDAEIARSGAKLRILPAIPEEEEETLGAFLDEVEEGRWSWTWHVP
                     EDVRRDAVREIRPWAEERFGPLDRIERYEVESVWRAYDLPG"
     gene            complement(1812..>2205)
                     /locus_tag="VF195_08535"
     CDS             complement(1812..>2205)
                     /locus_tag="VF195_08535"
                     /inference="COORDINATES: protein motif:HMM:NF020253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6580903.1"
                     /translation="EDTPLHVSGLERFVEWDQGADFLGRRRLEEIRDGDLLDRKLVGI
                     EIGGAPMTDEGALNDFWPLVQGDARVGKITAGAWSPRLEKNIGYAWVPIRWEAPGSTF
                     EAETPRGRVAVTVASLPFVDPTKDIPKS"
BASE COUNT          332 a          779 c          724 g          370 t
ORIGIN      
        1 ggtgagccag gaggcgtacc gttcggtctc gcccttggtg gcggcgaaga acgcgccctg
       61 cagctccttg gtgacggggc cgcggtgacc ctcgcccacc gtgcggtcgt ccacctcacg
      121 gatcggcgtg atctcggcgg cggtgccgga gaagaagcac tcgtcggcgt tgtagagatc
      181 ggtccggacc aggttctcct cggtcacgtc gtacccgagg tccctggcga tcgtgagcac
      241 cgtcccgcgc gtgatgccgt cgagacaccc cgcctggatc ggtggtgtgg tgagcttccc
      301 atcgcgcacg atgaagaggt tctcccccga tccgtcggtg atgaaccctt gctcgttcag
      361 catcaccgcc tcgtcgtacc ccgccttgag gccctcgatc ttcgcgagca cgccgttgat
      421 atattggccc gtcgccttgg ccgaggacgg cagcgcgttc tgcgagttcc ggcgccagga
      481 cgagatcttg atccggacac cgttctcgag agcgtcctcg ccgaggtacg cgccccacgg
      541 ccacaccgcg atgatcacgt tgaccggcgc gttcagcggg ttcacgccca tctcgccgta
      601 cccgcggacc acgagcggac ggacgtagca ggcgttcagc ccgttggcgc ggatgacctc
      661 tttggtcgcg tccgtgatcg cctcgatcga gtacgggatg tccatgtggt acagcttcgc
      721 cgagcggaac aagcgcttca gatgctcgtc caggtgccac accgcgggcc cccgagccgt
      781 ctcgtacgcc cggatcccct cgaagacccc gctgccgtag tgcacggcgt gggagagcac
      841 gtggatcttg gcatcccccc aatccacgag ctcgccatcc atccagatct tctcgacctc
      901 ggtgatcggc atctcggggc ctcctcggag cgggtcgtgg gtcgggtctt gttttcgtgt
      961 cttggcccgg gtgaggacag gatagcggct ggtcagggcg ccgggctcgg ttcagctctc
     1021 tcacccgggc aggtcgtatg cccgccagac gctctcgacc tcgtaccgct cgatgcgatc
     1081 gagcggcccg aagcgctctt cggcccaggg ccggatctcg cgaacggcgt ctcggcgaac
     1141 gtcctcgggt acgtgccagg tccaggacca gcgaccctcc tccacctcgt cgaggaaggc
     1201 cccgagcgtc tcctcctctt cctcagggat ggcgggcagg atgcggagct tggctcccga
     1261 tcgtgcgatc tcggcgtcga gcgcctcgtg gtcgccccac atcaggccga ccggatccgt
     1321 cgagaggccc gtgacctccg tgaaccgagc gcggatcgcc gagctcacct ggtcgaacgc
     1381 acccaggcag acgagcagga ggcctccggg gtggactacc cgaaccatct cggcgagcgc
     1441 cgacctccaa tcggggatca gatggagcac ccaccgcagg tacgcgccgt cgaaggtccc
     1501 atcaccgaac ggcatccggg tggcatcgcc caggacgaga gggaagaccg gttctcctcc
     1561 agccttctcg acgagcttgc cgagcatcgg cgcggagagg tccagacccg cgagcgagaa
     1621 gcctgcctcg tgaagccgta aggcgagcag ccccgtaccg acgccgacct ccaggacgcg
     1681 gccacgcgtc gcgagctcgg aggccagcag cgcgatcgtg cgggccatcg tctcgtcgga
     1741 gatcgcccgc gtccggtcgt agtggtcggc cgcgctgtcg aaccggatgg actcgctcac
     1801 gacgtcggag gctacgactt cgggatgtcc ttggtggggt cgacgaacgg caggctcgcg
     1861 accgtcacgg ccacacggcc tcgcggggtc tcggcctcga acgtcgaccc gggcgcctcc
     1921 catcggatcg gcacccatgc gtagccgatg ttcttctcga gtcggggcga ccatgcgccg
     1981 gccgtgatct tccccacacg cgcgtcgccc tggaccagcg gccagaagtc gttcagggcg
     2041 ccttcgtcgg tcatcggcgc accgccgatc tcgatgccca ccagcttgcg gtcgagcagg
     2101 tcgccgtcgc ggatctcctc caggcggcga cggccgagga agtcggcgcc ctggtcccac
     2161 tcgacgaacc gctcgagccc gctcacgtgc agcggcgtgt cctcg
//
LOCUS       DASUYD010000154        34208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1186248, whole genome shotgun sequence.
ACCESSION   DASUYD010000154 DASUYD010000000
VERSION     DASUYD010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 34208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1186248"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..765
                     /locus_tag="VF195_08540"
     CDS             <1..765
                     /locus_tag="VF195_08540"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HEX6580904.1"
                     /translation="LGEPPIEADRGRPMDTQKIARTAGILFLLTFITGIAGRLLFVSG
                     LGGTWDSLNFIPGAGSDTAIYWGSLAEFGVIATNIATAVVLYPILKRQSEIGAVGYLS
                     ARFVECAFILVGLISLLSVVTVRHDLTGAAGAAAGTALVGTYEWAFLFGPGLVAGLGN
                     GLLLGWLMYRSGLVPRRMAVLGLIGGPALAVGFVCVLFGVIDTGSVGQGAATAFEFVW
                     ELSLGIYLTIKGFRPSPITRSVDLREETPVPSLATV"
     gene            1047..1445
                     /locus_tag="VF195_08545"
     CDS             1047..1445
                     /locus_tag="VF195_08545"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6580905.1"
                     /translation="MFDRIVLAVDGSEHAGRAVGVAADLAKGGGEVHAIHVHEVGIVA
                     SIETSSEAKKLVDGVVGELQNAGVRASGSAIAARAGSVAPAILDAAKTFDANLIVLGT
                     RGLSDFAGLLLGSVAHKVIHHAECPVLVVR"
     gene            complement(1601..1981)
                     /locus_tag="VF195_08550"
     CDS             complement(1601..1981)
                     /locus_tag="VF195_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6580906.1"
                     /translation="MDVNTIYRDEVFSITGADTLEEAARLMHAEEIGSLVVMEGHRCV
                     GIITERDLTRAVAEGEDTHRVRVSGYMTIRPITVTPDTGVREAAAEMLETEVRHLPVG
                     VGGELIGMVSLRDLLNVVVEDENL"
     gene            complement(1997..4456)
                     /gene="gltA"
                     /locus_tag="VF195_08555"
     CDS             complement(1997..4456)
                     /gene="gltA"
                     /locus_tag="VF195_08555"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019190155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HEX6580907.1"
                     /translation="MGRNGAEQMGLRGPPSGSGYPIVARAQYGPTTFLLDVFAPDLAK
                     ACEPGQFVMVRIDETGERIPLTISDYDRDRGTVTIVVQAVGKTTFEMLALEAGERLLD
                     VVGPLGCASHVERRSRVVLVGGGLGVAPIFPLLRRHAKLGSGTISIVGFRSADRAFWT
                     DRFEALSDELRVATDDGSLGTMGTVADVLEQVLAEHDDVDEVVAIGPLAMMRACAEVT
                     RPRGIPTVVSLNSIMVDGTGMCGSCRVTVGGRMRFACVDGPDFDGHDVDFDELALRMG
                     RFAYEERACLDRYEEERRRLAELGIGGANPAELAARTRAFPMAGLPVPEPIPEALRPT
                     ATKNVNTISATRMPMPVLDASTRVQGFDEVALGYDLDAALVEADRCILCKNPKCIEGC
                     PVRIDIPGFIGALAAKDLRASYLILKASNTLPAVCGRVCPQEIQCEARCVVGKKRDPV
                     AVGRLERFVADFGAGRGWDEPPAGARSGTRVAVVGSGPAGLACAGDLARAGVEVTVYE
                     ALHVAGGVMKYGIPEFRLPNDTIDIEIEALEALGVRFELDAIVGQLYSLPQLLGEMGF
                     DAVFIGTGAGTPRFMGIPGESMNGVVSANEFLTRVNLMQGFRQPLYDTPVGMGKRVAV
                     IGAGNTAMDASRVALRMGAERVFLVYRRTANESPARAEELHHAIEEGIECRWLTNPTL
                     ILGDDDGWVTGLEVIRMELGEPDASGRPSPHPVPGTEELLEVDMVIEALGTAPNPTIS
                     RMTHDLETNERGYLVVDPSTQMTSVPGIFAGGDIVTGSATVILAMGAGRRAAAGILGF
                     LGVAEGKPTQHERIEAMSGAR"
     gene            complement(4810..5577)
                     /locus_tag="VF195_08560"
     CDS             complement(4810..5577)
                     /locus_tag="VF195_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6390 family protein"
                     /protein_id="HEX6580908.1"
                     /translation="MQTERLTGGGALLFARYAHPPNLLGYCGPADHLEFFEQATEGVV
                     DRGLHELTHAFDGAWPYLELIASANGIRDPLDHAVVHAYWIGNPLLERVDLSALGGSV
                     EARFRRRVGRGWDHLSTAILAGAVPHHSFHVLLVSPWIGLMRAGASAHPLHVMDRCRI
                     RTGRVIEVTGDVATVDVPALRWDGRELRVGSADPELATVKTDGRGFLPDLRPGDLVSL
                     HWDWVCDLLSAREARSLDGYTARHLSLANARTSVIPR"
     gene            complement(5577..6554)
                     /locus_tag="VF195_08565"
     CDS             complement(5577..6554)
                     /locus_tag="VF195_08565"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6580909.1"
                     /translation="MKLIDTRGPSSASPRRPSPFPHTPASGLALAFGTALVSGVAIFA
                     NGYGVTAFGSGGGVYTTAKNLVAAVVLMLVAGVATRARSEEGFTRPRTAAQWTGLLVV
                     AVIGGSVPFLLFFEGLARASSVQAAFLHKTLLIWVALLAWPLLRERIGPLHIVAIALL
                     LAGQAVLQGGVGEIGVGSGEAMILGATLLWSIEVIVAKRLLGSLSSLTVGTTRMALGC
                     VVLLAYLAVTGGVDDLLAVSATQWAWAALTGGLLAAFVATWYLALARARAIDVTTVLV
                     FGAVVTALLSMSVQGTEPQAAALALLALGTAIVAVAARRRGVSEPIRTG"
     gene            complement(6551..6793)
                     /locus_tag="VF195_08570"
     CDS             complement(6551..6793)
                     /locus_tag="VF195_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013611.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX6580910.1"
                     /translation="MCLGAIGVIREVFEENGLPMARVGGDVRTPEVCLLYVPAARPGD
                     HVLVELGFAVEVLDPAEAAEALHLRRASAGQDQEVS"
     gene            complement(6793..7263)
                     /locus_tag="VF195_08575"
     CDS             complement(6793..7263)
                     /locus_tag="VF195_08575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX6580911.1"
                     /translation="MNRAGAGGVTVIGIGNRFRRDDAAGLEVARRVRMLVDGDVRILE
                     LEGEPIDALDVWTAADEVILADAVAGTGAAGTVHLLDASEAPIPAPFRVKGTHAFSLA
                     DVVELARATELLPRSLVVYGIEGGDFSTGEGLTEPVARAVEDSVTEIVRRVGTR"
     gene            complement(7260..8573)
                     /locus_tag="VF195_08580"
     CDS             complement(7260..8573)
                     /locus_tag="VF195_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007168195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HEX6580912.1"
                     /translation="MTHGSVPSADAAIRVPGLARVEGEGGLEIHVRDGEVVEARLNIY
                     EPPRFFEAFLRGRRHTEPPDITARICGICPIAYQMSACRAIEDACGVTVDGPIATLRR
                     LIYLGEWIESHALHVYLLHAPDFLGYESAVHMAADHREVVERGLAMKKAGNRLMDVIG
                     GRSVHPINVRVGGFYRAPTRDELRTVLEPLERAREIAVETARWVSGFAFPSFERDYGF
                     VSLRHPTDYPITEGRVVSSDGLDIAIAEYDEHFVEEHVPHSTALHSRLRSTGNHYLVG
                     PLARFNLNADRLRPLARRAAEEAEIADGCRNPFQSIVVRAVELVQACDEAIDMIGSYE
                     PPDPPAVPVPPKAGAGTGATEAPRGLLYHRYVLDADGLIQDARIVPPTSQNQGTIEED
                     LVRFTDERLSLDREELTWQCEQAVRNHDPCISCATHFLDLRVVDA"
     gene            complement(8560..9336)
                     /locus_tag="VF195_08585"
     CDS             complement(8560..9336)
                     /locus_tag="VF195_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX6580913.1"
                     /translation="MAAPPRLAVWKFASCDGCQLTLLNCEDELLPVAGALEIASFPEA
                     SRAVIEGPYDLSLVEGSITTPSDAERIQEVRRQSRFLVTIGACATSGGIQALRNFKDV
                     DDFMRIVYAQPEYIETLQTSTAIADHVPVDFELRGCPIDRAQLLEVVGAFLNGRRPEV
                     PNHSVCIECKRRGTVCVMVAQGTACLGPVTHAGCGALCPTYDRGCYGCFGPMETPNTT
                     SLGHRFLQLGVREDDVVRVFRTFNANAEAFREESEARDAR"
     gene            complement(9336..10145)
                     /locus_tag="VF195_08590"
     CDS             complement(9336..10145)
                     /locus_tag="VF195_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX6580914.1"
                     /translation="MTPVPFTVRRSTRETADTVTLELDPGPSGFAFEAGQFNMLYALG
                     IGEVPISISGDPAIGAPLEHTVRSVGAVSAALCALRDGDAVGVRGPFGTDWRVAAAEG
                     SDVVIVAGGIGLAPLRPAIRGLLARREAYGRISVLYGARTPADLLFRDEVEGWRGRFD
                     VDVEVTVDAAGADWRGNVGVVPRLLARARFDAAGAVAMVCGPEVMMRFTVQALLDGGV
                     VPDRILVSLERNMQCAIKQCGHCQLGPYFVCADGPVMPYGLVAPWLRPREM"
     gene            complement(10191..10658)
                     /locus_tag="VF195_08595"
     CDS             complement(10191..10658)
                     /locus_tag="VF195_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6580915.1"
                     /translation="METLERLLAEHELFRGLDERYVTLLTGCAANVRFDAGAFLFREG
                     EPADRFFVLREGRVALEIDAPGRGPIVIQTLGEDDVLGYSWLFPPYRWRFDGRAIEPT
                     RALALDGQCLRGKCDDDPVLGYEMMKRFAGIALERLQATRLQLLDVYGHVGAR"
     gene            complement(10645..11778)
                     /locus_tag="VF195_08600"
     CDS             complement(10645..11778)
                     /locus_tag="VF195_08600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012699120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6580916.1"
                     /translation="MVVRAGDLNLLFEALRDRGYTVVGPTLRQEAIVYDELPSAAALP
                     VGWTDEQAPGSYRLKRREDDALFGYAVGPHSWKRFLFPPVTPLFSASRDGQAVLTEDP
                     EPPAPMYAFIGVRACELAAIDVQDRVFVGPGAQDPVYGARRSGAFLVAVNCGEAGGTC
                     FCVSMDTGPKVTQGYDLALTELLSEDEHLFLVEVGSDRGAEVIGDLPRAPATDEHLMA
                     ADQVVERTAASMGRSMETDGIKDLLYRNLEHPRWDDVAARCLACGNCTLACPTCFCSS
                     VEDRSDLAGDEAGRQRVWDSCFTLDHSYLHGGSVRGSTRSRYRQWMTHKLATWIDQFG
                     TSGCVGCGRCITWCPVGIDITEEVAAIRATDRGDREEASFGDA"
     gene            complement(12042..12692)
                     /locus_tag="VF195_08605"
     CDS             complement(12042..12692)
                     /locus_tag="VF195_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012920132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580917.1"
                     /translation="MSRPHVVVHVAVSLDGATTGFPVDVARFYELARAWREDVTLAGA
                     DTILVQESELAKAPRPGPALDAPILAVVDSRGRVREWEALRDCGYWSGVIALRSEATP
                     GQGATAVPELVVGADRVDLAAALEALADREGTRLVRVDSGGALIGALLRNHLVDELSF
                     LIHPCTVGSAGERRWHGPEPVGALRLTRTSVESLDDGLVWLRYRGSGSAASSPPRA"
     gene            complement(13578..14756)
                     /locus_tag="VF195_08610"
     CDS             complement(13578..14756)
                     /locus_tag="VF195_08610"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580918.1"
                     /translation="MNGGVRRAWLVTRREWDQRVRSRAFAIATAISIALVLAIILVPD
                     LVGSGRSRTVGLAGERSPGLPSIIRETGDQLGVSVEIRVFEDEKAGRTALRSDDVAVV
                     LVDQKELVWKSDVDEQLGAAVAEAVQSVERTRTADTLGLTPEQVERVLRPTNLTTTTL
                     EPATEEQTEREELAMIGVGVLLMAIAFYGGFLVVGVVEEKSSRVVEVVLAHLRPSELF
                     VGKIAGIGLAGLAQLGLIAVAALVALAFSANPSAETAPTTILWIVLWFILGYAFYAVL
                     YGTVGSLISRQEEAESIQFPVTAVLLVAYFLSMEAASSPDGSVALVASFVPFTAPMVM
                     TVRIAHGGVALWETILSLALMATTIVAMVQLAARVYTGAILRIGRRVRLREAWHGAEG
                     "
     gene            complement(14753..15652)
                     /locus_tag="VF195_08615"
     CDS             complement(14753..15652)
                     /locus_tag="VF195_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6580919.1"
                     /translation="MLEIRDLSKRYGNVVALDGTSFRVAPGRIVGFLGPNGAGKTTTM
                     RAIFGLVRPDHGEVLWRGTSVGPEARARFGYMPEERGLYPKMKVGDQLSYFARLSGLT
                     PAAANEATAAWLQRLGLLDRQGARLEELSHGNQQRVQLAAALVHDPVLAVLDEPFSGL
                     DPLGVEVLGEMLVETAATGVAVVFSSHQLDLVEDICQDVVIIDHGRVVLDGVVEDLKA
                     ASPRRSLEIAVNGDPWLPVLPEESMWVERNGRERVLVDAAVNLGEVLASARRAGDVTT
                     FSFEPPSLSDLFHAAVSDGGDGR"
     gene            complement(15791..16465)
                     /locus_tag="VF195_08620"
     CDS             complement(15791..16465)
                     /locus_tag="VF195_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HEX6580920.1"
                     /translation="MSAIFIPIQVLVFLTWPPPLDGTAVDWFELFGENRLVGLLDLDL
                     LLIADNVFLVSILLAMYVLLRRVNGPLMMIATSFGFLGIVFFVASNPAFEMLTLSERY
                     AAAATQAQRAAFLAAGEAMLATWEGTAFQMYYILGSITGIAIGAVMLRSAIFSNLAGW
                     MAILGNAVGLGLYIPTVGVYISVASVLFLEVWYILIARRLLLLGRRTIGAYRDREDAA
                     TATRMA"
     gene            complement(16768..17379)
                     /locus_tag="VF195_08625"
     CDS             complement(16768..17379)
                     /locus_tag="VF195_08625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580921.1"
                     /translation="MITTRTAEELSAPLGTEEEGYLVFSRGGSHLTIGVDGSMEDLFR
                     ASFDGKVPHTSVDRGTLTVKYRPTLHPPRGEMTLSGKIPWAIKARMGMSDVVADLADL
                     QLTDLEISGGASKIAVKLPRPNGTVRIRFGGGASNVELVRPAGVPVRLRIGAGTSRLE
                     VDDLRIGAAGGKTDWRSPAYDDAVDGRYDIEIGAGASKVTIRT"
     gene            complement(17412..17930)
                     /locus_tag="VF195_08630"
     CDS             complement(17412..17930)
                     /locus_tag="VF195_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HEX6580922.1"
                     /translation="MDGPVVRAAIRALEERDVDIVLPFVKAEGEEEVRRAFELVVAAR
                     EQGARAREAADRYLFDTVVRIHRAGEGAPFTGVKPAGLSVGPVIPVAERAIETGSAHE
                     LLELLGDALRDEVKERLDRVLELQIHADGPVAEAREYVEAMLGLQVWAHAVHGAIRSV
                     PHGTSEDREHVH"
     gene            complement(18089..19261)
                     /locus_tag="VF195_08635"
     CDS             complement(18089..19261)
                     /locus_tag="VF195_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580923.1"
                     /translation="MSAPKAIAIAGVNLRRLTRDRTGMFFVFVFPFLIILAIGAAFGA
                     GFTPRLGVVAAGSGPLGQDLRGRLTASEHVTIRGFADDEALRTAVERAEVEGGVVIPG
                     DYDARVRAGETVEVTYLARPTDTSRELEVAVRAVLDEQGATIRAARFALEEGSVATFE
                     EGLERARRVASAIPRVSVDARTPGGTAGLGTFATGAAQELVLFMFLTSLSASSMLIET
                     RRFGITRRMLASPTPVRSILVGEALGRYAIALVQGILIVVGTAILFQVEWGNLATTTA
                     VVLLFGLAATGAAMVMGSVLRSAAQAGAMGVFLGLVFAALGGSMVPLEIFPPVMARIA
                     HLIPHAWAIEALTASITDGTGPAGVATQLLVLAAYAVGLLAVATVLLRRAITSHAT"
     gene            complement(19258..20415)
                     /locus_tag="VF195_08640"
     CDS             complement(19258..20415)
                     /locus_tag="VF195_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6580924.1"
                     /translation="MRTALAIAGKDLRRRIRDRTAVLIAIILPFGLAWIFSLTLGAAG
                     SGDFEATLGVANEDGGGHLPEAFGELVARIDSVTLREVGSAAEAEHLIRAGTVDTAVV
                     FPSGFTAGVESGRGGRIDVIGSPDASIASLITVSLARSFASGLDAVSLSIATSSTPGT
                     EPDPAFVERARAVAPPATIGSSAAADTAASPSTFFAIGMAVFFLFFTVEFGVRGLLEE
                     REGGTLARLLVAPVRPSSVLAGKALASLAMGLVSTTLLVLASSWLLDADWGDPLGVVL
                     LVLGGVLAAVGVTSLVATLATTTAQAGAYVSIVAVVGGLLGGTFFPISEAGVFGAIRF
                     LSPQGWLMEGFQTLSYGGSLGEIRAPLLGVTAIAIATGAIASIRARGLVAR"
     gene            complement(20418..21407)
                     /locus_tag="VF195_08645"
     CDS             complement(20418..21407)
                     /locus_tag="VF195_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6580925.1"
                     /translation="MTRLQEVSIERTMDTHEPVLVAEALTKRYGDRLVVDGVGFTIDP
                     GETYGLLGPNGAGKTTTISMVCGLLSRDAGTVRLHGEELDTRTIDVKAGIGFVPQEIA
                     VYPDLTARENLRFFGKLYGLSGGPLTSRIGRVLETIGLTGRADDRADTYSGGMQRRLN
                     IGLGMLNDPTLLILDEPTVGVDPQSRNAILESVENLSAEGMAVLYTTHYMEEAERLCD
                     RVGILDEGDLKAEGTRRELIALVGEHDRLRVDARGDLPAAAARAATVPGVQEAVPREG
                     GIDVLARDVRGSLASLVRAIDQTGTTIVSMAVDEPNLEAVFLHLTGKALRDEG"
     gene            complement(21523..22026)
                     /locus_tag="VF195_08650"
     CDS             complement(21523..22026)
                     /locus_tag="VF195_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6580926.1"
                     /translation="MADEWASFREEHSHLRAGIESLRTVGDDVGRLDVAVLRYEVEDV
                     YRFLSHRLLAHIAVEEEVLYPAIARLNGYGAAAGIMRRDHAEVSDAVRELYALRQELE
                     RGTFGETEAAALRRVLYGLYQLLKVHMANEEEICLPALDQALSEGQVRELAEEIELYD
                     AAAQAAE"
     gene            22196..23179
                     /locus_tag="VF195_08655"
     CDS             22196..23179
                     /locus_tag="VF195_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018352489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX6580927.1"
                     /translation="MGDAESNVDDSPLGTVAEHVTTKVPIVAPTTSAPEARRSLVGER
                     FDAAAAIAVCEDAILRGVIRLEVLLAAPDEATAQDLMDAAPPVVAPGTDQEHAAWTAV
                     RRGEATLAVVDEAGRFLGFVPPHRLVAVLLEEHDEDLARIGGFLKDASSAQLASRERI
                     LRRYWHRLPWLLIGLAGALLASVIVGSFERQLSANVMIAYFIPGIVYMADAVGTQTET
                     LIVRGLSVGVSIREVVRRELLTGLLVGVTLAVLFLPVALWWWGDAELVVGTSIALLFA
                     CSIATIVAMFLPWLIHRIGWDPAFGTGPLATVIQDLLSVLIYFVVVFMLVG"
     gene            complement(23415..24740)
                     /locus_tag="VF195_08660"
     CDS             complement(23415..24740)
                     /locus_tag="VF195_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6580928.1"
                     /translation="MRPRAIVVGSGAGGATIAKELQGTFDVTVLEAGGDFRPLRFDLQ
                     TIERIKRSRLLFDPRLIRVPFPAMRARSTPQGIVLVHGTCVGGTTTVATGNGLRMDDD
                     LRAIGLDLDEEFDEINHEIPVSTAHRRGWHATTRRLFGVFEDMGLEPEPMPKMGDPER
                     CRHCGRCILGCPEGVKWDARRFVEDAALLGARVITRSPVERIVIESGAATGVIVRRGL
                     RERYVPADLVLLAAGGLGTPSILERSGIRCDPTLFVDPVLTIAGRRARAWQCKEIEMP
                     FVAQRDGFILSPYFDHLSFLLEPTWEVPARDLVGVMIKIADTNVGAVTMGGVEKGLTA
                     RDVTRIQEGVETCEEILRRFGVEDGKTIRGTLNAGHPGGTVPLTGADVRSMHPARLPE
                     NVYVADASLLPRALGNPPILTIVAMAKRIATLCTEHVTRPVRRQALARS"
     gene            complement(24737..25285)
                     /locus_tag="VF195_08665"
     CDS             complement(24737..25285)
                     /locus_tag="VF195_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580929.1"
                     /translation="MSMRLALLRFHLPALVRRAILRELISAIARAFERPCPPTSGLSS
                     EELLACAIDASHRWSEDALAGAADRSEVEERLFSEAFALGRRAKRRLRIGTEEEGLSA
                     AGVIYEAIGVELRAGGAGAIVIPRCAFARVYGPAACELMSAMDSGLIAGLTGAAGLRF
                     TERLTEGASACRAIVLHTGGVP"
     gene            complement(25282..28092)
                     /locus_tag="VF195_08670"
     CDS             complement(25282..28092)
                     /locus_tag="VF195_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX6580930.1"
                     /translation="MGRVIFLTGGAGFIGGRVAERLVRDPDTELVVLVLAPDGAPEGR
                     TPERVLARSWWDMPDLVSQIGRNVRPIFGDVRSARLGMRDDVHRELVRRVDVVIHAAA
                     DLRLEAPIDDLRVTNVEGVRHVLELAGEIDRDHGLDRLVHLSTAYVAGERRGTIEEGP
                     PTDRFGFASAYERSKYEGEMLVRAAMDELPIAIARPAMVVGDSRSGAITTFNTVYAPL
                     RLYMTGRLRVFPMRPDLRANIVPVDHVADAIVRMASDPTAAGATYHLTAPADSLPTGR
                     ELVDAVRIWSLEHLGVRLPRPIFAPILRPLLRGLRRSRGGVDPVLALLPYFQEHRTFV
                     RDNTDRSFGRYRFEWRACLPNLLAYAARYGFLHRTDRTVHEQILHRLTSERMPITYHD
                     VVRGRHRARDGADLRRDALAAVRSLRRIGVARGDRVAIVGPNGTRYLILDLAIGLMGA
                     VAVPLYPTSPPAEIEGMVIDSGARALFVGSPEVLARLPRLPDHVRIVSFARDVGASTA
                     TIQVVEAWHDFLAGSTDVGSAADDARAPVGPDDVATIRYTSGTTGAPKGVEFDHRAIR
                     WMGETMASLVPWTARNRPARYVSFLPMSHVVEGILATYGPAYLPAPVDVWFVEDLRDL
                     PRVLPTVRPVVFFGVPRVYEKLWTRFTASSAGRRYLARGRFARSLIRPLVRRGLLRAS
                     GLDRCAQLLVGSAPASERLLGAFRELGIEVHEAYGLTEAPLVALNPVGRNRIGTVGEP
                     LPDTRVRIAEDGEVLARGPQVTRGYVGDVDQPFHDGWLATGDLGRLTEARALVIEGRK
                     KDLLKTSYGKYVRASRIEGSLRDLPGVDEAMVVAEGRPYCTALLWSNEAHPSGPSRSS
                     LDAAIVSLNRDLPHPEQIKRWAILDDPPSVATGDLTVNSKLRRAVVLRRYAAEIDALY
                     GDGSARRSRVVIGEAPRTEMSV"
     gene            complement(28080..29366)
                     /locus_tag="VF195_08675"
     CDS             complement(28080..29366)
                     /locus_tag="VF195_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004029208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HEX6580931.1"
                     /translation="MSPQPPAASVDLEVIVMDAPRVLLVGYNGANNTGAEALLRADID
                     DVRAVLGDRVHITIPSLNPSNLRRYIREGPELEIVHIPTVFFRAMRRLVRDTDVVVLV
                     EGSAYMDTWTPALLWFFLWATHCAHAEGKPCLAYAVDAGTLSAVNRALVRRVASSTDL
                     IVTRSHAAANRLTSWGVTAPMRVTADNALTFTTDPADEGWVAREWPEAERAPVGLATV
                     DFFRFPVVIRPWGSADDCYRWPYYFSHSRSRRRAGEVLAAGYARTADRVVERHDRPVA
                     LIAMEELDDTLSGAILREMRHPGRARIFSAREYDGSQLTVLLRGLELLVTSRYHAAIL
                     SLAGRVPQIAVGHDLRLASLYDELRLDDVFLRATSPAVFSDLDRCIDFLIADPHHQDG
                     LLRAGHRALLDRAGRNRGLLRAFLAAHGYEAEPWAA"
     gene            complement(29506..29787)
                     /locus_tag="VF195_08680"
     CDS             complement(29506..29787)
                     /locus_tag="VF195_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2TM domain-containing protein"
                     /protein_id="HEX6580932.1"
                     /translation="MSARIEDQQGLRHRAEESLNKKLEFWTHLLAYVLINGVIVAIWA
                     MTGADFFWPAFPIAGWGIGLAFHAWDVYIERPPSEERIRREMDRLARQH"
     gene            complement(29793..30146)
                     /locus_tag="VF195_08685"
     CDS             complement(29793..30146)
                     /locus_tag="VF195_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008355893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6580933.1"
                     /translation="MGRPVTHFEVAVRDLDRAASFYEELFGWTVEDERMEGYRLVRTT
                     DEGIGGGLLKLPEGVFPYVTVYVGVDDLMASLHRAEELGGKVVVEPMPIPGVGSFAMF
                     QDPDGVMMGLFEEQG"
     gene            30475..30807
                     /locus_tag="VF195_08690"
     CDS             30475..30807
                     /locus_tag="VF195_08690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580934.1"
                     /translation="MNKRSAVLIALGLVLTLGIGGLAVSLGLTGPSPVSAASAQREPV
                     MRVQRKTVTVHRKAAAPSAVSLSVVAPSSKVEDDPSEDFEDEQGDEMENESEDHEDLD
                     EFETEADG"
     gene            30804..31106
                     /locus_tag="VF195_08695"
     CDS             30804..31106
                     /locus_tag="VF195_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580935.1"
                     /translation="MTGAPRRLASTGFVRLTAWASAAAAFLAAWGVLGTLPKPTASAA
                     VPETRPRQVVAVKKILRRVIIHEAAPAAAPPIVTYTPSSSGSSSPPAPTSTGGSAP"
     gene            31103..31978
                     /locus_tag="VF195_08700"
     CDS             31103..31978
                     /locus_tag="VF195_08700"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX6580936.1"
                     /translation="MRSWSFRAMGTTVRVLTSDDAPTDVVSASARRILRTFEREEGRF
                     SRFRSDSELSLVNAAAGRSMRVSIPFASVVRSALDAAASTEGLFDPTVLGAMDAIGYD
                     RDFDELLAGARGALHPIHPCGRWRDVTLEDDHLTLPAGVGLDLGGLVKGWTADLAAIR
                     GVDLGLRWVLVNAGGDLRIAGDAPRIDIAIEDPDDPRTELGRLWVRGGGAATSSTRSR
                     SWGDGFHHVIDPRTGQPSMTDLVQVTVVATTCAEAEIRATEVLVRGSDAAGEAAVSVT
                     SSGHVLLTVPIEEAA"
     gene            31975..32574
                     /locus_tag="VF195_08705"
     CDS             31975..32574
                     /locus_tag="VF195_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580937.1"
                     /translation="MTWLLARAAGIGAYLMLYLAVAWGLFATTSIVTKRVSKRSSILF
                     HGFVASAGLLLLVLHLTGLVLDRFVRFDLLDLLVPYRATYRPFALTMGIVSMYATVIV
                     LASSWSRKRLRPSVWRAIHLLAVPAFATALLHGVLAGSDSARVGITSLYWITGSSVLF
                     LVIVRGLTARPPRVRASQSSRRAGTFDRAAPRSRASLPG"
     gene            complement(32688..32858)
                     /locus_tag="VF195_08710"
     CDS             complement(32688..32858)
                     /locus_tag="VF195_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580938.1"
                     /translation="MSRDPVLRDARGRHLDEPAARAIHALFVGNGQAADGTFCFWDPL
                     VAALAIEPDPAS"
     gene            complement(32870..33280)
                     /locus_tag="VF195_08715"
     CDS             complement(32870..33280)
                     /locus_tag="VF195_08715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6580939.1"
                     /translation="MSLEEDHVKGIMSSPVVHVAPHATVAQALRAMIDRNIGAVVVVD
                     GASAVGVFTERDLTKGLLDDRDLLDREIGEVMSTPVTTVSPSDEVVFIFHLMTEKGIR
                     RLPVIDDGQLVGIVTERDLLRWVDAVATSPPASQ"
     gene            complement(33349..>34208)
                     /locus_tag="VF195_08720"
     CDS             complement(33349..>34208)
                     /locus_tag="VF195_08720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580940.1"
                     /translation="LTSVLVALIALRSSRRSAAPPTGSPLTVSASHVVAVTIEIDEAD
                     PGSPAVRRLVHETVLGIFRTAPDAREVVVRTRAGTILGRMSRETPMPRELTVSPMLHE
                     PHVRRTHVPDVLGGLYGSEPTPRPDRDRHTPGELAPPHRSLMDQFDLPEVVRNGIGDP
                     DDPIDLVRVILEAARVPCEIDGDLIRVGESALVILRASGDVVIGHEALNHAYLRIVRS
                     GAQRGLVIALGFVDPREIKRREMLAPQVLHAGPDGVQRMADAVCLGVDPLRFVAAIPV
                     SPGRPRLSP"
BASE COUNT         5400 a        12144 c        11516 g         5148 t
ORIGIN      
        1 ctgggagagc ctccgatcga ggcggacaga gggagaccga tggatacgca gaagatcgca
       61 aggacggcag gcatcctgtt cctgctcacg ttcatcacgg ggatcgcggg gagattgctc
      121 ttcgtgagtg gcctgggggg tacgtgggac agcctgaact tcatccccgg agcaggttcc
      181 gatacggcca tctattgggg ctctctcgcc gagttcgggg tcatcgccac gaacatcgcg
      241 accgcggtcg ttctctaccc gatcctcaaa cggcagagcg agatcggcgc tgtcggctac
      301 ctcagcgcac gcttcgtcga gtgcgcgttc atcctggtcg gcctcatcag cctcctgtcc
      361 gtcgtgaccg tgcgccacga cctgaccggg gctgccggag cggcggccgg caccgcgctc
      421 gtgggcacgt acgaatgggc gttcctgttc ggtcccggcc tcgtggccgg cctcgggaac
      481 ggcctcctgc tcggctggct gatgtatcgc tcgggactcg tgcctcgtcg gatggctgtg
      541 ttggggctca tcggtggtcc ggcgctcgcg gtcggcttcg tctgcgtcct gttcggagtg
      601 atcgacactg gatccgtcgg ccaaggcgca gcgaccgcgt tcgagttcgt atgggagctt
      661 tcactcggca tctatctgac gatcaagggc ttcaggccga gcccgatcac gaggtcggtg
      721 gatctccggg aggagacacc ggtgcccagc ttggcgacgg tctgacaagg ccgctgactt
      781 cgtcgtccgt tcatgcgctc ggcgtgagcg aaacgtcaag atgtccgatc ctcggcgcta
      841 tgtcttacga gcgccctgat gagcgcccgt cctttcacgc gaagaactgg ccgaccaggc
      901 tgaagtcgac cctgcatccg gcgaccgcct gatcgagatc gatgcgctcg gagaggatcg
      961 gcccttcagc tcttccgggc gccccgagtc cgacacgtct tggcgcgcga tgggataggt
     1021 tcttcacgtc aaaggggggt gatggcatgt tcgacaggat cgtcctagcg gtggacggct
     1081 cggagcacgc gggaagggct gtcggtgtgg ccgccgatct cgccaaagga ggcggcgagg
     1141 tgcacgccat ccacgtgcat gaggtgggga tcgtcgcgtc gatcgagacg tcgagcgagg
     1201 cgaagaagct cgtggacggc gtcgtcggcg agcttcagaa cgcgggggtg agggccagcg
     1261 ggtccgcgat cgcggcgcgt gccggatcgg ttgcacccgc gatcctcgac gccgcgaaga
     1321 ccttcgacgc gaatctcatc gtgttgggaa ctcgagggct atctgacttc gccggattgc
     1381 tgctcggaag cgtcgcacac aaggtgatcc atcacgccga gtgccccgtc ctcgtcgtgc
     1441 gctgaccggc gatccggatc gcggccctga tcctgcgcgc cgtgtcggtg ccggccggct
     1501 gcgggcgtcg acgacgacgc ggccgagccg ccccgacggc tccacgcgag gaccgccccg
     1561 cggaacgcgt cctcgctcgg ccctctcacc tcggagtggg ctagagattc tcgtcctcga
     1621 ccacgacgtt cagcaggtcg cgcagcgaaa ccatcccgat cagctcgccg ccgaccccca
     1681 cgggcaggtg gcgcacctcg gtctcgagca tctccgccgc cgcctcgcgg acccccgtgt
     1741 ccggggtcac cgtgatcggc cggatcgtca tgtagcccga gaccctgacc cggtgcgtgt
     1801 cttcgccctc ggcgaccgcc ctggtgaggt cccgctcggt gatgatcccc acgcaccgat
     1861 ggccctccat caccacgagc gacccgatct cctcggcgtg catgaggcga gccgcctcct
     1921 cgagggtgtc agcacctgtg atcgagaaca cctcgtcccg atagatcgtg ttgacgtcca
     1981 tccctgccac ctcctcctat ctcgcaccgc tcatcgcttc gatccgttcg tgttgcgttg
     2041 gcttcccctc agcgacgccg aggaagccga ggatcccggc agcggctcgc cgccccgcac
     2101 ccatggcgag gatcaccgtc gcggagccgg tcacgatgtc gccgcccgcg aagatcccgg
     2161 gtacggacgt catctgggtc gacgggtcga cgaccaggta cccccgctcg ttcgtctcga
     2221 ggtcgtgcgt catccgagag atcgtcgggt tcggcgccgt tccgagggcc tcgatcacca
     2281 tgtcgacctc caggagctcc tcggtcccgg gcaccgggtg cgggctcgga cgcccagacg
     2341 cgtccggctc gccgagctcc atccggatca cctcgagccc ggtgacccag ccgtcgtcgt
     2401 cgcccaggat cagagtcggg ttcgtgagcc aacggcactc gatgccctcc tcgatcgcgt
     2461 ggtgcagctc ctcggccctg gccggcgact cgttcgcggt ccgccggtag acgaggaaca
     2521 cgcgctcggc ccccatccgc agcgcaaccc ggctcgcgtc catcgcggtg ttccccgccc
     2581 cgatcaccgc gacgcgcttg cccatcccga ccggcgtgtc gtacagcggc tgccggaacc
     2641 cctgcatcag gttcacgcgc gtaaggaact cgttcgccga cacgacgccg ttcatcgact
     2701 ccccggggat ccccatgaac cgcggcgttc cggcgccggt cccgatgaac accgcgtcga
     2761 agcccatctc gccgaggagc tgcggcaacg agtagagctg gccgacgatc gcgtcgagct
     2821 cgaaccggac gccgagggcc tcgagggcct cgatctcgat gtcgatcgtg tcgttcggca
     2881 gccggaactc ggggatcccg tacttcatca ccccgccggc gacgtggagg gcctcataga
     2941 ccgtgacctc gacgccggcg cgcgcgaggt ctccggcgca cgccagtccc gcaggcccgg
     3001 agccgacgac cgccacgcgc gtgccggacc gcgcgcccgc cggcggctcg tcccagccgc
     3061 gcccggcgcc gaaatcggcc acgaagcgtt cgagccgacc gaccgcgacc ggatcacgct
     3121 tcttcccgac cacgcaccgc gcctcacact ggatctcctg cgggcagacg cgcccgcaca
     3181 cggccggcag cgtgttcgag gccttcagga tcaggtagga cgcccggagg tccttcgcgg
     3241 cgagtgctcc gatgaatccg gggatgtcga tccggaccgg acagccctcg atgcacttcg
     3301 ggttcttaca gaggatgcag cggtcggcct cgacgagcgc cgcatcgaga tcgtagccca
     3361 acgccacctc gtcgaacccc tgcaccctgg tcgacgcgtc gaggacgggc atcggcatcc
     3421 tggtcgccga gatggtgttc acgttcttcg ttgcggtggg gcggagcgcc tcggggatcg
     3481 gctcgggcac cggcagcccg gccatcggga acgcgcgggt cctcgcggcg agctcggccg
     3541 ggttcgcgcc gccgatcccg agctcggcga gccgccggcg ctcctcttcg taccggtcga
     3601 ggcacgcgcg ctcctcgtac gcgaaccgcc ccatccgcag ggcgagctcg tcgaagtcga
     3661 cgtcgtggcc gtcgaaatcc ggaccatcga cgcacgcgaa gcgcatgcgc ccgccgaccg
     3721 tcacccggca gctgccgcac atccccgtgc cgtcgaccat gatcgagttg aggctcacga
     3781 cggtggggat cccgcgcggg cgcgtgacct cggcgcaggc ccgcatcatc gcgagcgggc
     3841 cgatcgcgac gacctcgtcc acgtcgtcgt gctcggcgag gacctgctcg aggacgtcgg
     3901 ccaccgtccc catcgtcccg agcgagccgt cgtccgtggc gacccgcagc tcatcggaga
     3961 gtgcttcgaa acggtcggtc cagaacgcac ggtccgccga gcggaagccc acgatcgaga
     4021 tcgtgccgga gccgagcttc gcgtgacgcc gcaacagcgg gaagatcggc gcgactccga
     4081 ggccgccgcc gacgagcacc acgcgcgatc ggcgctcgac gtgcgatgcg cagccgagcg
     4141 gtcccacgac gtcgaggagc cgctcgccgg cttcgagcgc cagcatctcg aacgtggtct
     4201 tcccgaccgc ctgcacgacg atcgtgaccg ttccccggtc tcggtcgtag tccgagatcg
     4261 tgagcgggat acgctcgccg gtctcgtcga tccggaccat cacgaactgg ccgggctcgc
     4321 aggccttcgc cagatcgggc gcgaagacgt cgaggaggaa ggtcgtcggg ccgtattgcg
     4381 ccctcgcgac gatcgggtag cccgatcccg atggcggccc cctcaggccc atctgctcag
     4441 ctccgtttcg tcccatgacg tccttaggtc agggtgcgca ggacccagtg ggggtcacta
     4501 gggccgaacg acccctccga gacggaccga acgacgtgcg gggcagcccg gaaccggtgg
     4561 gccaggatgg gtggcgcggt ctggaagagc ccgtgggggt caccggacgg ctgccgtcgg
     4621 ggacctcctg ggccaggctg cggatggatc gatcgcaggc cgccgcgcgt cccgcacccg
     4681 ggttatggga gccctcgctc agacgtgttg gtcgacgtag atcgtgtccc cgtcaggccc
     4741 gtcacgatga agcctggccc agtggacgat tcgatcccga tgagggcgcc cagggacgac
     4801 cgggcggctt caacgcggga tgacgctcgt tcgcgcgttc gcgagggaca gatgcctagc
     4861 cgtgtaccca tcgagcgaac gagcttcccg ggcgctcagc aggtcgcaca cccagtccca
     4921 gtggagcgat acgagatcgc caggccggag gtcggggagg aacccacggc cgtcggtctt
     4981 gaccgttgcg agctccgggt cagccgagcc gacccggagc tcgcgaccgt cccatcggag
     5041 cgcgggcacg tcgaccgttg ccacgtcacc cgtgacctcg atcacgcgcc ccgttcggat
     5101 ccggcatcga tccatcacgt ggagcggatg cgccgaagct ccggcccgca tcaggccgat
     5161 ccacggcgac accagcagga cgtggaaact gtggtgcggc acggcgccgg ccaggatggc
     5221 cgtcgacagg tggtcccacc ctcgacccac gcgccggcgg aaccgcgctt cgacagagcc
     5281 tccgagcgcc gacaggtcga cgcgttcgag tagcgggttg ccgatccagt acgcgtggac
     5341 gacggcgtgg tccagcggat cgcggatccc gttggccgac gcgatcagct cgagataggg
     5401 ccacgcgcca tcgaacgcgt gcgtcagctc gtgcaaccca cggtcgacca caccctcggt
     5461 ggcctgctcg aagaactcca ggtggtcggc gggtccgcag tatccgagga ggttcggcgg
     5521 gtgcgcgtag cgtgcgaaga gcagcgctcc gcctcccgtc aaccgctctg tctgcatcag
     5581 cccgtccgga tcggctccga cacgcctcgc cgcctcgccg ccaccgcgac gatcgccgtc
     5641 ccgagcgcga gcagcgccag tgcggccgcc tgtggctcgg tgccctgcac gctcatgctc
     5701 aggagcgcgg tgacgacggc tccgaacacg agcacggtcg tcacgtcgat cgcccgcgcc
     5761 cgtgcgagcg cgaggtacca cgtcgcgacg aaggcggcga gcagtccgcc cgtcagcgcg
     5821 gcccacgccc attgcgtggc cgacaccgcg agcaggtcgt cgaccccgcc cgtcaccgcg
     5881 aggtacgcga gcagcacgac gcagccgagg gccatgcgcg tggtcccgac ggtgagcgac
     5941 gagagggatc cgagaagccg cttcgcgacg atcacctcga tcgaccacag cagcgtcgcc
     6001 cccaggatca tcgcctcgcc gctgccgacg ccgatctcgc cgacaccacc ctgcagcacc
     6061 gcctggcccg ccagcaggag cgcgatcgcg acgatgtgga gcggtccgat ccgttcgcgg
     6121 aggagaggcc aggcgagcag cgccacccag atgagcagcg tcttgtggag gaacgccgcc
     6181 tgcaccgacg aggcgcgtgc gagcccctcg aagaacagta ggaacggcac gctgccgccg
     6241 atcacggcga cgacgagcag accggtccac tgcgccgcgg tgcgtgggcg cgtgaagccc
     6301 tcctccgagc gcgctctcgt cgccacccca gcgacgagca tcaggacgac ggcggccacg
     6361 aggttcttcg cggtcgtgta gacgccgccg cccgagccga acgccgtcac gccgtaaccg
     6421 ttcgcgaaga tcgcgacccc gctgacgagg gccgtcccga aggcgagcgc gagcccgctc
     6481 gccggcgtgt gggggaacgg ggacgggcgc cgcgggctcg cgctcgaggg cccccgcgta
     6541 tcgatgagct tcatgagacc tcctgatcct gtcctgccga ggctctgcgc aggtgcagcg
     6601 cctccgcagc ctccgccggg tcgagcacct cgaccgcgaa gcccagctcg accagcacgt
     6661 ggtcgccggg ccgggccgcc ggcacgtaca gcaggcacac ctcgggcgtg cggacgtcac
     6721 cgcccacgcg cgccatcggc agcccgttct cctcgaacac ctctcggatc accccgatcg
     6781 ctccgaggca catcagcgcg ttcccacccg gcgaacgatc tcggtcaccg aatcctcgac
     6841 cgcgcgcgcc accggctctg tgagcccctc gccggtggag aagtcgccgc cctcgatccc
     6901 gtacacgacg agggagcgcg ggagcagttc ggtggcccgc gccaactcga cgacgtcggc
     6961 gaggctgaac gcgtgggtcc ccttcacgcg gaacggcgcg gggatcggcg cttccgaggc
     7021 gtccagcaga tgcacggtgc cggccgcgcc ggtaccggcc accgcgtcgg caaggatgac
     7081 ctcgtcggcg gccgtccaca cgtccaacgc gtcgatcggc tcgccctcca gctccaggat
     7141 gcgcacgtcg ccgtcgacga gcatccgaac ccttcgggcc acctccaagc ccgcggcgtc
     7201 gtctcgccgg aaccggttgc cgatcccgat cacggtgacg ccgccggcgc cggcccggtt
     7261 catgcatcca ccacccggag gtcgaggaaa tgcgtcgcgc acgagatgca cgggtcgtgg
     7321 ttccgaacgg cctgctcgca ctgccaggtg agctcctcgc gatcgagcga gagccgctcg
     7381 tcggtgaagc ggacgaggtc ctcctcgatc gtgccctgat tctgggaggt cggcgggacg
     7441 atccgcgcgt cctggatcag accatccgcg tccaggacgt accggtggta cagcaggccg
     7501 cggggcgcct cggtggcgcc ggtgccggcg ccggccttcg gcggcaccgg gaccgccggt
     7561 ggatccggcg gctcgtacga tccgatcatg tcgatcgcct cgtcgcacgc ctgcacgagc
     7621 tcgacggcgc gcaccacgat gctctggaag gggttccggc acccatccgc gatctcggcc
     7681 tcctcggccg cgcggcgggc gagcggccgg agacgatccg cgttcaggtt gaagcgcgcc
     7741 agcggaccca cgaggtagtg gttcccggtc gaccgtagtc gcgagtggag ggccgtcgag
     7801 tgcggcacgt gctcctccac gaagtgctcg tcgtactcgg cgatcgcgat gtcgaggccg
     7861 tcgctcgaga cgacccggcc ctcggtgatc gggtagtccg tgggatgtcg gagcgagacg
     7921 aagccgtagt cccgctcgaa ggaggggaag gcgaaccccg agacccaccg ggccgtttcc
     7981 accgcgatct cgcgcgcccg ttccagcggc tcgagcaccg tccgcagctc gtcgcgggtc
     8041 ggagcgcggt agaacccgcc gacccggacg ttgatggggt gaacggagcg cccgccgatc
     8101 acgtccatca ggcggttgcc ggccttcttc atcgcgagtc cccgctcgac cacctcgcgg
     8161 tgatcggcgg ccatgtggac cgcgctctcg tacccgagga agtcgggggc gtgcaggagg
     8221 tacacgtgca gggcgtggct ctcgatccac tcgccgaggt agatcagccg ccggagcgtc
     8281 gcgatcggtc catccaccgt caccccgcac gcgtcctcga tcgctcggca ggcgctcatc
     8341 tggtacgcga tcgggcagat cccgcagatc cgcgcggtga tgtccggcgg ctcggtgtga
     8401 cgccgtcccc ggaggaacgc ctcgaagaac cggggcggct cgtagatgtt cagccgagcc
     8461 tccaccactt cgccgtcgcg gacgtggatc tcgaggccgc cctcgccttc cacgcgggcg
     8521 aggccgggga cgcggatcgc ggcgtcggcg ctcggcacgc taccgtgcgt cacgggcctc
     8581 gctctcctcg cggaacgcct ccgcgttcgc attgaacgtg cggaacacgc gcacgacgtc
     8641 gtcctcacgg accccgagct ggaggaagcg atgccccagc gacgtcgtgt tcggcgtctc
     8701 catcggcccg aagcatccgt agcagcctcg gtcgtacgtg gggcagagcg cgccgcagcc
     8761 ggcgtgcgtg accggaccga ggcatgcggt cccctgcgcg accatcacgc agacggtccc
     8821 gcgccgcttg cactcgatgc acacgctgtg gttcgggacc tcgggtcgtc gaccgttcag
     8881 gaacgccccg accacctcga gcagctgggc ccggtcgatc gggcatccgc ggagctcgaa
     8941 gtccaccggc acgtgatctg cgatcgcggt cgaggtctgg agggtctcga tgtactcggg
     9001 ctgcgcgtac acgatccgca tgaagtcgtc gacgtccttg aagttccgca gcgcctggat
     9061 cccgccggac gtggcgcacg ccccgatcgt gacgaggaac cgcgactgcc ggcggacctc
     9121 ttggatccgc tcggcgtcag aaggggtcgt gatcgaaccc tcgacgagtg agaggtcgta
     9181 cgggccctcg atcacggcgc gcgatgcctc ggggaacgag gcgatctcga gcgcgccggc
     9241 gaccgggagg agctcgtcct cgcagttcag cagcgtcagc tggcacccgt cgcacgaggc
     9301 gaacttccac acggccaacc ggggtggcgc cgccatcaca tctcccgcgg gcggagccag
     9361 ggcgcgacga gcccgtaggg catcaccggg ccgtcggcac acacgaagta cgggccgagc
     9421 tggcagtgac cgcactgctt gatcgcgcac tgcatgttcc gctcgagcga gacgaggatc
     9481 cggtcgggga ccacgccgcc gtccagcaac gcctggaccg tgaaccgcat catcacctca
     9541 ggtccgcaca ccatcgccac ggcaccggcc gcgtcgaatc gtgccctcgc gagcagccgg
     9601 ggcaccaccc cgacgttgcc tcgccagtct gctccggccg cgtcgaccgt gacctcgacg
     9661 tccacgtcga accgtcctcg ccaaccttcg acctcgtcgc ggaacaggag gtccgccggc
     9721 gtccgagcgc cgtagaggac cgagatccgg ccgtaggcct cgcgacgggc gaggagcccg
     9781 cggatcgctg gccggagcgg cgcgaggccg atcccgccgg cgacgatcac gacgtccgac
     9841 ccttccgccg ccgcgacgcg ccagtccgtg ccgaacggcc cgcggacgcc gaccgcgtcg
     9901 ccatcgcgaa gagcgcagag tgcggccgaa acggcgccga cgctccggac cgtgtgctcg
     9961 agcggcgcgc cgatcgccgg gtcgccgctg atcgagatcg ggacctcacc gatccccagg
    10021 gcgtacagca tgttgaactg ccccgcctcg aacgcgaatc cggagggacc cggatccagc
    10081 tcgagcgtca cggtgtcggc cgtctcgcgc gtcgagcggc gaaccgtgaa cggcaccggt
    10141 gtcatcggat cggggcgatc gacgatcgac gtgccgacgg agaccatcgc tcagcgcgct
    10201 cccacgtgtc cgtacacgtc gaggagctgg agtcgggtcg cctgcaagcg ctcgagcgcg
    10261 atccccgcga agcgtttcat catctcgtag ccgagcaccg ggtcgtcgtc gcacttgccg
    10321 cgcaggcact gcccgtccaa cgcgagcgcc cgagtgggct cgatcgcgcg gccgtcgaag
    10381 cgccaccggt acggggggaa caaccacgag tacccgagca catcgtcctc gcccagcgtc
    10441 tggatcacga tcgggccgcg gcccggcgcg tcgatctcga gcgccacccg cccctcgcgc
    10501 agcacgaaga acctgtcggc gggctcgccc tcacggaaca ggaaggctcc ggcgtcgaag
    10561 cggacgttcg ccgcgcagcc cgtgagcaac gtcacgtatc gctcgtcgag gccgcggaag
    10621 agctcgtgct cggcgagcag gcgctcaagc gtctccaaag gacgcctcct cccgatcgcc
    10681 ccgatccgtc gcgcggatcg cggcgacctc ctcggtgatg tcgatcccga cagggcacca
    10741 cgtgatgcag cgcccgcatc cgacgcaccc actggtcccg aactgatcga tccacgtcgc
    10801 gagcttgtgc gtcatccact ggcggtagcg cgagcgcgtg ctgccgcgaa cgctgccgcc
    10861 gtgcaggtag gagtggtcga gcgtgaagca cgaatcccag acacgctggc gccccgcctc
    10921 gtcgcccgcg agatcgctcc ggtcctccac cgaggagcag aagcaggtgg ggcaggcgag
    10981 cgtgcaattg ccgcacgcca ggcagcgagc ggcgacgtcg tcccagcggg ggtgctcgag
    11041 gttccggtag agcaggtcct tgatgccgtc ggtttccatc gagcggccca tgctcgcggc
    11101 ggtgcgctct acgacctggt cggccgccat gaggtgctcg tcggtggccg gcgcgcgggg
    11161 aaggtctccg atcacctccg cgccgcgatc gctccccacc tcgacgagga acaggtgctc
    11221 gtcctccgag agcagctcgg tgagggcgag gtcgtaccct tgggtgacct tcgggccggt
    11281 gtccatcgag acgcagaagc acgttccgcc agcctcgccg cagttcaccg cgaccaggaa
    11341 cgcgccgctc cgccgggcgc cgtagacggg atcctgcgct cccgggccga cgaacacccg
    11401 gtcctggacg tcgatcgcgg caagctcgca cgcacggacg ccgatgaacg cgtacatcgg
    11461 cgccggcggc tccggatcct cggtgagtac cgcctgtccg tcgcgcgacg cggagaacag
    11521 gggcgtgacc ggtgggaaca ggaaccgttt ccacgaatgc gggccgacgg cgtagccgaa
    11581 gagggcgtcg tcctcgcggc gcttcagtcg gtagctgccg ggagcctgct cgtccgtcca
    11641 gcccaccggg agcgcggcgg cagaaggcag ctcgtcgtag acgatcgcct cctgtcggag
    11701 cgtcggtccg accaccgtgt acccgcggtc acgaagggcc tcgaacagga ggttcaggtc
    11761 gccagcacgg acgacgatcg gtgactcggt gcgtaccgaa gcatctccgc ggctgtgcac
    11821 ggatccctcg tcatcgcgac gttgcccgcc catccgtatg ccctcccgag cgcggtccga
    11881 gccactggct gccctccgac gtggagcgta ccccttccta ccgcggagcc ggtcccgacg
    11941 gggaccaagc gccgaaaggc accaccacca gagctacccg tccgccacgg aacaggccgc
    12001 ctgagctgcg aaggtatggt cagccggtgc attcgctgtg ctcaggcccg tggcggtgac
    12061 gacgcggccg atccgctgcc gcggtagcgg agccagacga gcccgtcgtc gagcgactcc
    12121 acgctcgtcc gtgtcaaccg aagggcgccg acgggttcgg ggccatgcca tcgacgctcg
    12181 ccggcagacc cgaccgtgca ggggtggatc aggaagctca attcgtccac gagatggttc
    12241 cggagcagtg caccgatcaa cgctccgcca ctgtccacgc gcaccagccg tgttccctcc
    12301 cggtccgcga gcgcttcgag cgcagcggcc aggtccaccc gatccgcgcc cacgacgagc
    12361 tcggggacgg ctgtcgctcc ctgaccgggc gtggcctcgg agcgcagcgc gatgacgccc
    12421 gaccagtagc cgcagtcccg cagcgcttcc cactcgcgga ctcgccctcg gctgtcgacc
    12481 accgcgagga tcggagcgtc gagcgcgggc ccgggtcggg gcgccttggc cagctcgctc
    12541 tcctggacaa ggatcgtgtc ggcgccggcg agcgtgacgt cctcccgcca ggcccgggcg
    12601 agttcgtaga agcgcgccac gtcgaccggg aagcccgtgg tcgcaccatc cagcgaaacg
    12661 gcgacgtgga ccaccacgtg cggacggctc atctgacgac ccccctcgca gcgtcaggct
    12721 cccaggcgac ggcacggtgt gcgacgatca tcgcatcgca ccgtcctcgg agaggatcac
    12781 gaacacggcg agtcggatgc cgcccgagcc gcagcgagcg agatcatcgt gacggcgtcg
    12841 acgttcggcg tctcggtcag cagaagacat ggtgcgggag cgggccttgg ccttcgcgat
    12901 cggctccgtg tcgtgccctg caactaccga gccccttgcc gttcttgaga tgtcatcccc
    12961 tgagcgattc tgagatcggc atgccgaccg cgagcgcccc gaggaggaga ttggcgaaga
    13021 gccctaccca gccgccacgg aagcctctcg agacggctcg gacaccgaga taggttccga
    13081 ccgcgaacgg agccatcatc atggctccca ggacggaacc cttccagagc tcccaatgac
    13141 ctgtgctccg aggttgaaga cccttcccgc cagggcgaat cccacgaacg gagccatgaa
    13201 cacccacccg acgaaatacc gggagagtct cgcctcgcga gcacctggag tcgactcgac
    13261 cgatgacgac gaccgcgatc cgcgactcgg aacacgagta gttgccatct gacctcccgt
    13321 cgatccctcc ttccaggaga tcacgatccg ggggtcatct gaaccgacgc acgtcccgct
    13381 tctatcaggc ccttcggcca cgagatccgc gaacgaccga ccatgttcct cacgcgtgac
    13441 ggagcatgtc cgcggatgcc ggccggccgt tccaggagag cgccgatcgg acagccctac
    13501 caccccgcga gatcgtcggc tcaggtggtc gaagccggcc gccggcatgg atcaccccgg
    13561 tcggcggagc gtgggctcta tccttccgcg ccgtgccacg cctctcgcag gcggacccga
    13621 cggccgatgc ggaggatcgc acctgtgtag acccgtgcgg cgagttgcac catcgcgacg
    13681 atcgtggtcg ccatcagcgc gagcgagagg atcgtttccc acagggcgac gccaccgtgc
    13741 gcgatgcgga cggtcatcac catcggagcc gtgaagggaa cgaaggacgc gacgagcgcc
    13801 accgaaccgt cgggcgagct cgcggcctcc atggagagga agtacgcgac caggagaacg
    13861 gccgtgacgg ggaactggat gctctccgct tcttcctgcc gcgagatcag cgagccgacc
    13921 gtcccataca gcaccgcgta gaaggcatag cccaggatga accagaggac gatccacagg
    13981 atcgtggtgg gagcggtctc ggccgacgga ttcgccgaga acgccaacgc cacgagcgcg
    14041 gcgaccgcga tcaggccgag ctgcgccaat cctgcgagcc cgatgccggc gatcttgccg
    14101 acgaagagct ctgacggacg gagatgagcg agtacgacct ccaccacgcg gctggacttc
    14161 tcttcgacga cgccgacgac gaggaagccg ccgtagaagg cgatcgccat gagcagcaca
    14221 cccacgccga tcatcgcgag ctcctcgcgc tcggtctgct cctccgtggc cggttccagc
    14281 gtggtcgtcg tgaggttcgt gggtcgcagc acgcgctcga cctgctccgg cgtgaggccg
    14341 agcgtatcgg ctgtccgcgt gcgctcgacg ctctgcaccg cttccgccac tgccgcgccg
    14401 agctgctcgt cgacgtcgga cttccagacg agctccttct ggtcgacgag aaccaccgcc
    14461 acgtcgtccg atcggagcgc ggtcctgcct gccttctcgt cctcgaatac ccggatctcg
    14521 accgagacgc cgagctggtc gccggtttcg cggatgatgg agggcagtcc gggggatcgt
    14581 tcgccggcca gacccacggt ccgggatcgt cctgagccga cgaggtccgg cacgaggatg
    14641 atggccagga cgagagcgat cgagatggcg gtcgcgatcg cgaacgcccg cgatctcacc
    14701 cgctgatccc attcccggcg ggtgaccagc cacgcgcgcc gcacgccgcc gttcatcgtc
    14761 cgtcccctcc atccgacacc gccgcgtgga agaggtcgct cagcgacggt ggctcgaaac
    14821 tgaacgtcgt cacgtccccg gcccgtctgg cactcgcgag gacctctccc aggttgaccg
    14881 cggcatcgac gagcacccgc tctcgcccgt tccgctcgac ccacatcgac tcctcgggta
    14941 ggaccgggag ccaggggtcc ccgttcaccg cgatctcgag cgatcgacga ggggaggccg
    15001 ccttcaggtc ctcgacgacg ccgtcgagca cgacccggcc atgatcgatg atcaccacgt
    15061 cctggcagat gtcctcgacg aggtcgagct gatggctcga gaacacgacc gcgacacccg
    15121 tcgcggcggt ctccaccagc atctcgccga ggacctccac accgagcggg tcgagaccgc
    15181 tgaacggctc gtcgaggacc gcgagcacgg gatcgtggac gagggccgca gccagctgca
    15241 cccgctgctg attgccgtga gacagctcct cgagccttgc accctgccgg tccaggaggc
    15301 cgaggcgctg gagccacgcc gccgtggcct cgttcgcggc ggccggcgtc agccccgaga
    15361 gtctggcgaa gtagctgagc tggtcgccga ccttcatctt cgggtacagc ccgcgttcct
    15421 ccggcatgta tccgaatcgg gctcgcgcct ccgggccgac ggatgtcccg cgccacaaga
    15481 cctctccgtg gtccggacgc accagcccga agatcgcccg catcgtggtg gtcttccccg
    15541 cgccgttggg gccgaggaac cccacgatcc ttcctggcgc cacgcggaaa ctcgttccat
    15601 ccagcgcgac gacgtttccg taccgcttcg agaggtctcg gatctcgagc atcttcgtcg
    15661 ctccctcacg ccatcatgcg gactcggcat ccgaccgccc gagccgttcg tcctgtcttc
    15721 gaagggacgt tcgccctcga cgctcggtca tcgctccgcg aagtccgctt ggggcccggg
    15781 taccagcggc tcaggccatc ctcgtcgcgg tggcggcgtc ctcgcgatct cggtaggcac
    15841 cgatcgtgcg acgtcccagc agcagcagcc gccgagcgat caggatgtac cacacctcca
    15901 ggaacagcac ggaggcaacg gagatgtaga cgccgaccgt cgggatgtac agacccaacc
    15961 ccacggcatt ccccaagatg gccatccatc ccgccaagtt gctgaagatg gcgctccgga
    16021 gcatcaccgc cccgatcgcg atgccggtga tcgatccgag gatgtagtac atctggaaag
    16081 ccgtcccctc ccaggtggcg agcatggctt cgccggccgc taggaaggca gcacgctggg
    16141 cttgggtcgc cgccgccgcg tatcgctcgc tcaacgtcag catctcgaag gccgggttcg
    16201 acgcgacgaa gaagacgatt ccgaggaatc cgaaggacgt cgcgatcatc atgaggggac
    16261 cgttgacacg cctgagcaag acgtacatcg cgaggaggat cgagacgagg aacacgttgt
    16321 ccgcgatcag caagaggtcg aggtcaagga gcccgaccag ccggttctcg ccgaagagct
    16381 cgaaccagtc gacggcggtg ccgtcgagcg gtgggggcca ggtgaggaac accagcacct
    16441 ggatcgggat gaatatcgcc gacatcaacg ccccgactcc ggcgatctcg tagaggcccc
    16501 tccagcgtgg atcgcgggag tccgcctctc gatccggatc cgtgtcggcc cctccgcttc
    16561 catgacgtgc tcgaggcgat cctctctcgt cacgtccgtg ggggtcctgg accgagtcga
    16621 tccgccggtc gacgtcaact ctcatctcgc cacctcccac gctgaccgta ggacggggat
    16681 ccgcggcaga gctgggctgg aggacccggg atccggggga ccgacggcgg ctccttccgc
    16741 cgccgccggc cttccctctg aagaccctca ggtccggatc gtgaccttgc tcgctcccgc
    16801 cccgatctcg atgtcgtatc gaccgtcgac cgcatcgtcg taggcggggc ttcgccagtc
    16861 cgtcttccct ccggcggccc cgatccgaag atcgtcgact tcgagtctcg aggttccggc
    16921 cccgatgcgc agccggacgg ggactcccgc cggccggacg agctccacgt tgctcgcacc
    16981 gccgccgaac cggatccgca ccgtgccgtt cgggcggggc agcttcacgg cgatcttcga
    17041 ggcgcctccg gagatctcca gatccgtgag ttgcaggtcc gcgaggtctg cgacgacgtc
    17101 agacatcccc atccgcgcct tgatcgccca cgggatcttg cccgagaggg tcatctcacc
    17161 gcgcggtgga tggagggtgg ggcggtactt gacggtcagg gtgcctcgat cgaccgaggt
    17221 gtgaggcacc ttcccgtcga agctcgcccg gaacagatcc tccatcgatc cgtccactcc
    17281 gatcgtcagg tgcgagccgc cgcgggagaa gaccaggtac ccctcttcct ccgtccccag
    17341 gggcgcgctc aactcttctg cggttctcgt tgtgatcacc ttcgtcatcc cctttctcgc
    17401 cgatgatgag gtcagtggac gtgctcccgg tcctcggacg tgccgtgtgg gaccgagcgg
    17461 atcgcgccgt gcacggcgtg cgcccagacc tgcagcccga gcatcgcctc gacgtactcg
    17521 cgggcctcgg caaccgggcc gtccgcatgg atctggagtt ccagcacgcg atcgagccgt
    17581 tccttgacct cgtcgcgcag ggcgtccccg agcagctcga gcaactcgtg ggcagaacct
    17641 gtctcgatcg cgcgctcggc gaccgggatc accggaccga ccgacagccc cgccggcttc
    17701 acgccggtga acggggctcc ctcgccggca cgatggatcc gcacgaccgt gtcgaagagg
    17761 taccggtcgg cggcctctcg agccctagcc ccctgttccc gcgcggcgac cacgagctcg
    17821 aaggcacgcc gcacctcctc ctctccctcc gccttcacga agggaaggac gatgtcgacg
    17881 tcccgctcct ctagtgctcg gatggcggcc cgaacgacgg gcccatccat cgaatcgcaa
    17941 tgcggtggca tctcgatgca cctcctcgag agaacgttgt ctcagctccg acggtacggc
    18001 gcccgctggc gggcggcttg ggccgttcgg cccacgttcg cagggtcgcc cggagatgac
    18061 ggtcacgccg cggacatccg aacgagcgct acgtcgcgtg cgaggtgatc gcccgccgga
    18121 ggagcaccgt agccacggcg agcagcccca cggcgtacgc tgccagcacg aggagctgcg
    18181 tggcgacgcc ggcgggtccc gtgccgtccg tgatcgaggc ggtcagcgcc tcgatcgccc
    18241 aggcgtgggg gatcaggtgc gcgatccgcg ccatgaccgg cgggaagatc tcgagcggca
    18301 ccatgcttcc tccgagcgcc gcgaagacca gcccgaggaa gacgcccatc gcgccggcct
    18361 gggccgcgct gcgaagcacc gagcccatca ccatcgcggc gccggtcgcg gcgaggccga
    18421 agagcagcac gacggcggtc gtggtggcca ggttccccca ctcgacctgg aagaggatcg
    18481 ctgtaccgac cacgatcaag atgccttgga cgagggcgat cgcgtaccgg ccgagtgctt
    18541 cccccacgag gatcgaacgc accggcgtgg gcgacgcgag catccggcgg gtgatcccga
    18601 accggcgggt ctcgatcagc atcgaggacg ccgagagcga cgtgaggaac atgaacagga
    18661 cgagctcttg ggccgcgccg gtcgcgaacg tgcccagccc cgcggtcccg ccgggtgtcc
    18721 gggcgtcgac cgagacacgc gggatcgccg acgcgacccg cctcgcccgc tcgagcccct
    18781 cctcgaacgt cgcgacgctt ccctcctcca gcgcgaagcg ggcggcccgg atcgtggcgc
    18841 cttgctcgtc gaggaccgcc cggaccgcga cctcgagctc ccggctggtg tcggtgggcc
    18901 gcgcgaggta ggtgacctct acggtttcgc cggcccggac acgggcgtcg tagtcgccgg
    18961 ggatcacgac gccgccctcg acctctgcgc gttcgacggc ggtccggagt gcctcgtcgt
    19021 cggcgaaccc ccggatcgtg acgtgctcac tggcggtgag ccgcccgcgc aggtcctggc
    19081 cgagcggacc ggacccggcg gccacgacgc cgagcctcgg ggtgaacccg gctccgaagg
    19141 cggctccgat cgcgaggatg atcaggaagg ggaacacgaa cacgaagaac atccccgtgc
    19201 ggtcgcgcgt caggcggcgg aggttgacgc cggcgatcgc gatcgccttc ggggcgctca
    19261 tcgtgccacc agcccccggg cacggatcga ggcgatcgcg ccggtggcta tcgcgatcgc
    19321 cgtcacgccg aggagcggcg cccggatctc gccgagcgag ccgccgtacg acagcgtctg
    19381 gaagccctcc atcagccacc cttgtgggga gaggaaccgg atcgctccga agacccccgc
    19441 ttcggagatc gggaagaacg tcccaccgag cagcccgccg acgaccgcca cgatggacac
    19501 gtaggccccc gcctgcgcgg tcgtcgtcgc gagcgtggcg acgagcgacg tgacgccgac
    19561 cgccgcgagg acgccgccga gcacgaggag cacgacaccg agcggatcgc cccagtcggc
    19621 gtcgagcagc cagctgctcg ccagcacgag tagcgtcgtg ctcacgaggc ccatcgcgag
    19681 gctcgccagg gccttccccg cgaggacgga cgacggacgg accggcgcga ccagcaggcg
    19741 cgcgagggtc ccgccctcac gctcctcgag caggccgcgg acaccgaact cgacggtgaa
    19801 gaagaggaag aacaccgcca tgccgatcgc gaagaacgtc gagggggagg cggcggtgtc
    19861 cgcggcggcg ctcgagccga tcgtcgccgg aggcgcgacg gctcgggcac gctcgacgaa
    19921 tgcggggtcc ggctccgtcc cgggagtcga tgacgtggcg atcgataggg acacggcgtc
    19981 gagccccgat gcgaaggacc gggcgaggga caccgtgatc agcgacgcga tgctcgcgtc
    20041 cggcgagccg atcacgtcga tccgaccgcc acgtccgctc tccacaccgg cggtgaagcc
    20101 ggatgggaac accacggccg tatccaccgt gccggcgcgg atcaggtgct ccgcctccgc
    20161 ggcggatccg acctcgcgga gcgtgacgga gtcgatccgc gcgacgagct cgccgaacgc
    20221 ctcggggagg tgcccgccgc cgtcttcgtt cgcgaccccg agggtcgcct cgaagtcacc
    20281 ggagccggcg gcaccgagcg tgaggctgaa gatccacgcg aggccgaacg ggaggatgat
    20341 cgcgatcagc acggcggttc gatcgcggat ccgtcggcgg aggtctttgc ccgcgatggc
    20401 cagcgccgtg cgcatcgcta cccctcgtcc cgcagcgcct tgcccgtgag atgcaggaac
    20461 acggcctcga ggttcggctc gtcgaccgcc atcgagacga tggtcgtccc ggtctggtcg
    20521 atcgcgcgga cgagcgaggc gagcgaaccc cgaacgtcac gcgccaggac gtcgatgcca
    20581 ccctcgcgcg ggacggcttc ctggacgccc ggcaccgtcg cggcacgcgc cgcggccgcg
    20641 gggaggtcgc cgcgcgcgtc cacccggagc cggtcgtgct ctccgaccag cgcgatcagc
    20701 tcccgcctgg tgccctcggc cttcaggtct ccctcgtcga ggatcccgac gcgatcgcag
    20761 agccgctcgg cctcttccat gtagtgcgtc gtgtagagga ccgccatccc ttccgcggac
    20821 aggttctcga ccgactcgag tatcgcgttg cggctctgcg ggtcgacgcc gaccgtcggc
    20881 tcgtcgagga tcaagagggt cgggtcgttc aacatgccga ggccgatgtt cagccggcgc
    20941 tgcatcccac ccgagtacgt gtccgcgcgg tcgtcggcgc gtccggtgag gccgatcgtc
    21001 tcgaggacgc gcccgatcct cgatgtgagc ggcccgcccg acagcccgta cagcttcccg
    21061 aagaaccgca ggttctcccg ggcggtgaga tccgggtaga ccgcgatctc ctgcggtacg
    21121 aagccgatcc cggccttcac gtcgatggtg cgggtgtcga gctcctctcc gtggagcctc
    21181 accgtgccgg catcgcggct cagcagaccg cagaccatcg agatcgtcgt ggtcttcccc
    21241 gcgccgttcg ggccgaggag cccatacgtc tcgccggggt cgatcgtgaa cccgacgccg
    21301 tcgaccacga ggcggtcccc gtaccgcttc gtgagggctt cggcgacgag cacgggctcg
    21361 tgggtgtcca tggtccgctc gatgctcacc tcctggagcc tcgtcacgat acgcccccct
    21421 ctgcctccat cggcaccacc cccggtcgga gcggtcgcgg tgtcggacgc cgtccgggtc
    21481 gtgggtctcg gctggacgag gatcgcagcg ccggcgtcct gttcactcgg cggcctgcgc
    21541 cgctgcatcg tagagctcga tctcctcggc gagctcccgg acctgtccct cggacagggc
    21601 ctggtcgagc gcggggaggc agatctcttc ttcgttcgcc atatgaacct tgaggagttg
    21661 atagagcccg tagagcacgc ggcggagcgc tgcggcctcg gtctcgccga acgtcccgcg
    21721 ctcgagctct tgcctcaagg cgtagagctc ccggacggcg tctgagacct cggcatggtc
    21781 ccgtctcatg atccccgccg cggccccgta gccgttcagc cgggcgatcg ccgggtacag
    21841 aacctcctct tccacggcga tgtgggcgag gagccgatgc gagaggaagc gatatacgtc
    21901 ctcgacctcg tacctcagca ccgccacgtc cagccgaccg acgtcatccc cgacggttcg
    21961 gaggctctcg atgccggctc ggagatgcga gtgctcttcc cggaacgacg cccactcatc
    22021 cgccacgcgg tctcatctcc tctgagatcg atccaccccc agcgtacgga cggacctctt
    22081 cgggcacagg gccgatggga ccggatcacg agggaccttg cgcggctcag cccttgcgga
    22141 gatgtcggcg aaccgccagg gtctcggtga ctcgacagcg aaacggaggt acctgatggg
    22201 cgatgcggag tcgaacgttg atgacagccc gctgggcacc gttgcggagc atgtgaccac
    22261 gaaggtgccg atcgttgccc ccaccacgtc cgctcccgaa gcccgtcgtt cgctcgtcgg
    22321 cgagcggttc gatgccgccg cggccattgc cgtctgcgaa gacgcgatcc tgcgaggcgt
    22381 gatccggctg gaagtgctcc tcgcggctcc cgatgaggct acggcgcagg acctgatgga
    22441 cgccgccccg ccggtcgtcg cccccggaac cgatcaggag cacgctgcct ggacggctgt
    22501 acggcgaggc gaggcgacgc tcgccgtcgt cgacgaggcc ggccgattcc tggggttcgt
    22561 cccaccgcac cgcttggtgg ccgttctcct cgaggagcac gacgaggacc tggcccggat
    22621 cggtggcttc ctgaaggacg cgtcctccgc acagctcgcc agccgcgaga ggatcctccg
    22681 ccgctactgg catcgcctgc cctggttact gatcgggctc gcaggtgcgc tgctcgcctc
    22741 cgtcatcgtc gggtcgttcg aacggcagct ctctgcgaac gtgatgatcg cgtacttcat
    22801 ccccgggatc gtctacatgg ccgacgccgt gggcacgcag accgagacgc tcatcgtgcg
    22861 cggactgtcg gtcggcgtgt cgatccgaga ggtggtccgt cgcgagctgc tcaccgggct
    22921 gctcgtcggc gtcacgctgg ccgtactgtt cctcccggtc gccctctggt ggtggggcga
    22981 tgccgaactg gtggtgggga cgtcgatcgc cctgttgttc gcgtgctcga tcgccacgat
    23041 cgtcgcgatg ttcctgccgt ggctgatcca tcgcatcggc tgggatccgg cgttcggaac
    23101 ggggccgctg gccacggtga tccaggacct cctgtcggtg ctgatctact tcgtggtcgt
    23161 gttcatgctg gtcggctgat cccggacgag cgtgcgctca tcgctcaccg cgcttggggc
    23221 cacaggagca ccagacggcc ctacaggaga tcttcggcag gcgcgaccgc cttcctcgat
    23281 ccggtcgtgc cgatgccgta cggaggctgg gcgaccggcc gcgatccgac ccgacctcgg
    23341 ccgacgacgc gatcgacgga tgccctcaca accgtcgacg accggtcacg tcgcgtggcg
    23401 acgatgccgg cgggctagga gcgagcgagg gcctgtcggc ggacggggcg tgtcacatgt
    23461 tcggtgcaga gcgtcgcgat gcgcttcgcc atggctacga tcgtgaggat cggcgggttg
    23521 ccgagtgccc tggggagcag cgaggcatcc gcgacgtaca cgttctccgg taagcgcgcc
    23581 ggatgcatcg accggacgtc tgctcccgtc agcgggacgg tcccgccggg gtgcccggcg
    23641 ttgagcgtcc ccctgatcgt ctttccgtcc tcgacgccga agcgccgcag gatctcctcg
    23701 caggtctcga cgccttcctg gatccgtgtg acgtctctcg ccgtgaggcc cttctcgacg
    23761 ccgcccatcg tcaccgcccc cacgttcgtg tccgcgatct tgatcatcac cccgacgaga
    23821 tccctcgctg gcacctccca ggtcggctcg agaaggaagc tcaggtggtc gaagtagggc
    23881 gacaggatga agccgtcccg ctgtgccacg aacggcatct cgatctcctt gcactgccac
    23941 gcgcgcgccc gacggcccgc gatcgtgagg acggggtcga cgaagagcgt cggatcgcac
    24001 cggatgcccg agcgctccag gatcgacggt gttccgagcc cgcccgcggc cagcagcacg
    24061 aggtcggccg gcacgtaccg ctcgcgcaac ccgcgccgaa cgatcacgcc ggtcgctgcg
    24121 ccactctcga tcacgatccg ctcgacgggg gaccgcgtga tcacacgagc cccgagcagg
    24181 gcggcatcct ccacgaaccg cctcgcgtcc cacttcaccc cctcggggca accgaggatg
    24241 cagcggccgc agtgccggca gcgctcgggg tcgcccatct tcggcatcgg ctcgggctcg
    24301 aggcccatgt cctcgaagac tccgaacagc cgacgcgtcg tggcgtgcca gccacggcgg
    24361 tgcgccgtcg agacggggat ctcgtggttg atctcgtcga actcctcgtc gaggtcgagg
    24421 ccgatcgcac ggaggtcatc gtccatccgg agaccgttcc cggtggcgac cgtggtcgtc
    24481 ccgccgacgc acgtgccgtg cacgaggacg atcccctgcg gggtcgaccg ggcgcgcatc
    24541 gccgggaacg gcacgcggat cagcctgggg tcgaagagga gacgcgagcg cttgatccgc
    24601 tcgatcgtct gcaggtcgaa gcgcagcggt cggaagtcgc cgccggcttc cagcaccgtg
    24661 acgtcgaagg tgccctggag ttccttggcg atcgtcgccc ctccggcgcc gctcccgacc
    24721 acgatcgcac gcggcctcac gggacccctc ccgtgtggag gacgatcgcg cggcaggccg
    24781 acgcaccttc cgtgagtcgc tcggtgaagc ggaggccggc ggcaccggtg agaccggcga
    24841 tcaggccgga atccatcgcg gacatgagtt cgcaggcggc tggcccgtac acgcgagcga
    24901 acgcgcaccg gggtatcacg atcgctccag cacccccggc gcggagctcg accccgatcg
    24961 cttcgtagat cacgccagca gcggagagcc cttcctcttc cgtgccgatc ctcagacggc
    25021 gcttcgcgcg ccgaccgagc gcgaacgcct cggagaagag acgctcctca acctcggatc
    25081 ggtcggcggc tccagcgagc gcgtcctcgc tccaccggtg ggatgcgtcg atcgcgcacg
    25141 cgaggagctc ttcggacgag aggcccgacg tgggtgggca aggccgctcg aaggcccgcg
    25201 cgatcgccga gatcagctcg cgcaggatcg cgcggcgcac cagcgccggc agatggaacc
    25261 gcaagagcgc cagccgcatg ctcataccga catctccgtc cgaggtgcct cgccgatcac
    25321 cacgcgcgac cgtcgtgccg atccgtcgcc gtacagggcg tcgatctccg cggcgtaccg
    25381 tcggaggacg acggcccgtc gcagcttcga gttcacggtc aggtcgcccg tcgcgacgga
    25441 tggcgggtcg tcgaggatcg cccaccgctt gatctgctcc ggatggggga gatcacggtt
    25501 caaggagacg atcgccgcgt cgagggacga gcgcgacggg ccagaagggt gggcttcgtt
    25561 cgaccagagg agagccgtgc agtacggccg cccctccgcg acgaccatcg cctcatccac
    25621 gcccggcagg tcccggaggc tcccctcgat ccgcgatgcc cgcacgtact tgccatagga
    25681 cgtcttgagc aggtccttct tgcggccctc gatcacgagc gcgcgtgcct ccgtcagtcg
    25741 cccgagatca ccggttgcga gccagccgtc gtggaacggc tggtcgacgt cgccgacgta
    25801 cccgcgggtc acctgcggtc cgcgggcgag cacctcaccg tcctcggcga tcctcacgcg
    25861 ggtgtccggc aacggctccc cgacggtccc gatccggttc cgaccgaccg ggttcagcgc
    25921 caccagcgga gcctcggtca gcccataggc ctcgtgtacc tcaatcccga gttcgcggaa
    25981 ggcccccagc agccgttcgc tcgccggggc cgatccgacg agcagctgcg cgcaccggtc
    26041 gagtccggac gctcgcagga ggccacggcg gacgagcggg cggatcagcg atcgcgcgaa
    26101 ccgtccccga gccaggtacc gacgtcctgc cgacgatgcc gtgaacctgg tccagagctt
    26161 ctcgtagacg cggggaactc cgaagaacac gaccggccgg accgtcggca gcacgcgtgg
    26221 gaggtcgcgg aggtcctcga cgaaccagac gtcgaccggc gccggcagat acgcgggacc
    26281 gtacgtcgcg aggatcccct cgacgacgtg actcatcggg aggaaggaga cgtaccgcgc
    26341 gggcctgttc cgagccgtcc acggcacgag cgaagccatc gtctcgccca tccagcggat
    26401 cgctcggtgg tcgaactcca cacccttcgg agcgccggtc gttccggacg tgtaacggat
    26461 cgtcgcaacg tcgtcgggtc cgacgggcgc cctggcgtcg tcggctgccg agccgacgtc
    26521 ggtggacccg gcgaggaagt cgtgccacgc ctcgacgacc tggatcgtgg ccgtcgaggc
    26581 gccgacgtcc cgagcgaacg acacgatgcg gacgtgatcc ggaagccggg gcagccgcgc
    26641 gagcacctcc ggagagccca cgaagagcgc ccgcgcgccg ctgtcgatca ccatcccctc
    26701 gatctccgcc ggcggactcg tgggatagag cggaaccgcg acggctccca tgagaccgat
    26761 cgcaaggtcg aggatgaggt accgggtccc gttcggtccc acgatcgcca cgcgatcgcc
    26821 gcgtgcgacc ccgatacgcc ggagcgaccg caccgcggcg agagcgtccc gtcggaggtc
    26881 ggccccatcc cgcgcgcgat gccgccctcg caccacgtcg tggtacgtga tcggcatccg
    26941 ttcgctcgtg aggcgatgca ggatctgctc atgcaccgtc cggtcggtgc gatggaggaa
    27001 gccgtaccga gccgcgtagg cgagcaggtt cgggaggcac gcgcgccact cgaaccggta
    27061 ccgcccgaac gatcggtcgg tgttgtcacg aacgaacgtc cggtgctcct ggaagtaggg
    27121 gagcagcgcg agcaccgggt cgaccccgcc cctcgagcgt cggaggccac ggagcagggg
    27181 ccgtaggatc ggtgcgaaga tcggtcgcgg aagtcgcacc ccgaggtgct cgagcgacca
    27241 gatcctgacg gcgtcgacca gctcccgacc cgtcgggagc gagtccgctg gagcggtcag
    27301 atggtaggtc gccccggctg cggtcgggtc gctcgccatg cggacgatcg cgtcggccac
    27361 gtgatcgacc ggaacgatgt tcgcgcggag gtcggggcgc atcgggaaga cccggagccg
    27421 ccccgtcatg tagaggcgca gcggtgcgta gaccgtgttg aaggtcgtga tcgcgccgct
    27481 cctggagtct ccgacgacca tcgcggggcg ggcgatcgcg atcgggagct cgtccatcgc
    27541 ggctcgaacg agcatctcgc cctcgtactt gcttcgctcg taggcactcg cgaacccgaa
    27601 ccgatccgtg ggcgggccct cctcgatcgt tcccctgcgc tctccggcga cgtatgcagt
    27661 cgagagatgc acgagccggt cgaggccatg gtctcggtcg atctcgccgg cgagctcgag
    27721 gacgtgacgc accccttcca cgttcgtgac ccggaggtcg tcgatcggtg cctcgagccg
    27781 caggtcggcc gccgcatgga tcaccacgtc gacccgacgg accagctcac gatgaacgtc
    27841 gtcgcgcatc ccgaggcggg cggaccgcac gtcaccgaag atcgggcgca cgttccggcc
    27901 gatctgcgag acgaggtccg gcatgtccca ccacgaccga gccagcaccc gttcgggtgt
    27961 ccggccttcg ggggcaccat cgggcgcgag aacgaggacg acgagctcgg tgtcgggatc
    28021 acgcaccagc cgttccgcga cacggccccc gatgaatccc gccccacccg tgaggaagat
    28081 cacgcggccc atggctcggc ctcgtacccg tgcgcggcga ggaacgcacg gaggagccct
    28141 cgattacgcc cggcgcgatc gaggagtgct cggtgtccgg ctctgagcag gccgtcttgg
    28201 tgatgcggat ccgcgatcaa gaagtcgatg cagcgatcga ggtcggagaa gaccgccggg
    28261 gacgtcgcgc gcaggaagac gtcgtcgagt cggagctcgt cgtagaggct cgcgaggcgg
    28321 aggtcgtggc cgaccgcgat ctgcggcacc cggcccgcca acgacaggat cgccgcgtga
    28381 taccgcgacg tgaccaacag ctcgaggccc cgcaacagaa cggtgagctg tgacccgtcg
    28441 tactcgcgag cggagaagat ccgagcgcgc ccagggtggc gcatctctcg caggatcgcg
    28501 ccggagagcg tgtcgtcgag ctcctccatc gcgatcaacg cgaccggacg gtcgtgacgc
    28561 tccacgaccc ggtcggcggt cctggcgtag ccagccgcga gaacctcacc cgcccggcgc
    28621 cgcgaacgtg agtgggagaa gtagtagggc cagcgatagc agtcgtcggc acttccccac
    28681 ggacggatca ccacggggaa ccggaagaag tccacggtcg cgaggcccac cggcgcccgt
    28741 tcggcctcgg gccactcgcg agcgacccac ccctcatctg ctggatcggt cgtgaacgtg
    28801 agcgcgttgt cggcggtaac tcgcatcggt gccgtgacgc cccagctcgt gaggcggttc
    28861 gccgccgcgt ggctcctggt cacgatgagg tctgtcgagc tggccacgcg ccggacgagt
    28921 gcccggttca ccgccgagag ggtgccggca tccaccgcat aggcgaggca cggcttcccc
    28981 tcggcgtggg cgcagtgggt cgcccagagg aagaaccaga ggagcgccgg cgtccaggtg
    29041 tccatgtagg cgctcccttc gacgaggacg acgacgtccg tgtcgcggac cagccgccgc
    29101 atcgccctga agaacacggt cgggatatga acgatctcga gctccggccc ttcacggatg
    29161 taccgacgca gattggacgg gttcagcgat gggatcgtga tgtgaacacg atcaccgagc
    29221 accgccctca cgtcatcgat gtcggcacgc aggagggctt cggcacctgt gttgttcgct
    29281 ccgttgtatc cgaccaacag gacccgaggg gcatccatca cgatcacctc caggtccacg
    29341 ctcgccgcag gcggctgcgg cgacaccggc cggatgaccc cccgcctgag ggacctgcgg
    29401 cgtcccgtcg cggatgagac agcggccggt cgccgtgggt cacgccaggc agcagtcgag
    29461 ggcgggcagc ggagctgtcc gccctcgaac gtgcaggcgc ctgcgctagt gctgcctcgc
    29521 cagccgatcc atctcccgcc ggatccgctc ctcggacggt ggccgctcga tgtagacgtc
    29581 ccaggcgtgg aacgccaatc cgatgcccca gcccgcgatc gggaacgccg gccagaagaa
    29641 gtccgcgccg gtcatcgccc agatcgccac gatgaccccg ttgatgagga cgtacgcgag
    29701 gaggtgagtc cagaactcca acttcttgtt caggctctct tccgctcggt ggcgcagccc
    29761 ttgctgatcc tcaatccgtg cgctcatctt cctcacccct gttcctcgaa cagccccatc
    29821 atgaccccgt ccggatcctg gaacatggcg aacgaaccca cgccggggat cggcatcggc
    29881 tcgacgacca ccttcccgcc cagctcctcg gccctgtgca gggacgccat gaggtcgtcc
    29941 acccccacgt agaccgtgac gtaagggaac acgccctccg gcagcttcag aaggccaccg
    30001 ccgatgcctt cgtccgtcgt acgaacgagg cgatacccct ccatccgctc atcctcgacc
    30061 gtccagccga acagctcctc gtagaacgag gccgctcggt ccagatcccg gaccgcgact
    30121 tcgaagtgcg tcactggccg tcccatctct tcctcctcgt gtgctctgat gtgatgttct
    30181 cgccgggcca gtcgccacct cagggccgaa cgtcccggga tccgcccgac ttccaggctg
    30241 tccgaacggt gaaggtgccg gccaccacac ttcgggggcg atcgacgagc aactcacgtc
    30301 ctcggggagc cgcgtgatcg cgttcgccgt tcgagaggga acggacgtcg atcccacgtc
    30361 cgccggagga cgtctgcctt ctggttagtg acgtcttaac gcccctttgg cagggccttt
    30421 gacggggcgt ggctagcctg gctgcccggt acatccgtcc gaaggaggag aaccatgaac
    30481 aagcgctcgg cggtactgat cgctctcggt ctcgtgctca cgttgggcat cggagggctc
    30541 gcggtgtcgc tcggcttgac cggcccgtcg cccgtcagtg cggcgtcggc ccaacgcgag
    30601 ccagtgatga gggtgcagcg gaagaccgtg acggttcatc ggaaggccgc ggcgccatcg
    30661 gctgtgtcgt tgtccgtggt ggctcccagc tcgaaggtcg aagacgaccc ttccgaggac
    30721 ttcgaggacg agcagggaga cgagatggag aacgagagcg aagaccacga ggacctcgat
    30781 gagttcgaga ccgaggccga cggatgaccg gagctcctcg ccgcctcgcg agcacggggt
    30841 tcgtgcggct gacggcatgg gcctccgccg cagccgcctt cctcgccgca tggggcgtct
    30901 tgggaacgct gcccaagccg acagcctccg ccgccgtccc tgagacccgt ccgcggcagg
    30961 tcgtggctgt caagaagatc cttcggcgcg tgatcatcca cgaggcggcg cccgcagctg
    31021 ctcctccgat cgtgacctac acgccgtcgt cgtccggctc gtcctcgccg ccggcaccga
    31081 cctccacggg gggttcggcg ccatgaggag ctggagcttc cgtgcgatgg gcacgaccgt
    31141 gcgggtcctg acgagcgacg acgcacccac cgacgtcgtc tcggcgtctg cgcggaggat
    31201 cctgcgaacg ttcgagcgag aggagggccg cttctcgcgg ttccgctccg acagcgagct
    31261 ctccctcgtc aacgctgcgg cgggacggtc gatgcgggtc tctatcccgt tcgcttcggt
    31321 ggttcgatcc gcgctcgacg cggccgcctc caccgagggc ctgttcgacc ccaccgtgct
    31381 cggagcgatg gatgcgatcg ggtacgatcg cgacttcgac gagctcctgg cgggagctcg
    31441 gggggcattg catccgatac atccctgtgg gcgctggcgc gacgtgaccc tcgaggacga
    31501 tcacctgacc ctcccggccg gcgtcggcct cgacctcggg gggttggtca aggggtggac
    31561 agccgatctc gctgcgatcc gtggcgtcga cctgggactc cggtgggtgc tcgtcaacgc
    31621 cggaggcgat ctgcggatcg cgggcgatgc gccccggatc gacatcgcga tcgaggatcc
    31681 agatgacccc cgcacggagc tcggtcgact ctgggtgcgc ggaggcgggg ccgccacctc
    31741 ctcgacccgt tcgcgatcgt ggggagacgg gttccatcac gtgatcgacc cccggacggg
    31801 tcagccctcc atgaccgacc tcgtgcaggt caccgtcgtc gcgacgacct gcgccgaggc
    31861 ggagatccgg gcgacggagg tgttggtgcg gggatcagat gcggcggggg aggcggccgt
    31921 atcggtcacc tcctcggggc acgtcctcct gacggtcccg atcgaggagg cggcatgacc
    31981 tggcttctcg cgcgggcggc gggcatcggt gcctacctca tgttgtatct ggcggtcgcc
    32041 tggggcctct tcgcgaccac gtcgatcgtg acgaaacgcg tatcgaagcg gtcttccatc
    32101 ctcttccacg gcttcgttgc gagcgccggg ctgctgctcc tggtgctcca cctcacgggg
    32161 ttggtgttgg atcggttcgt ccggttcgac ctgttggatc tcttggtccc gtatcgagcg
    32221 acgtatcgcc ccttcgcgct cacgatgggg atcgtctcga tgtacgcgac ggtgatcgtc
    32281 ctggcttcct cgtggtcgcg gaagcggctg cggccatccg tgtggcgcgc gatccacctc
    32341 ctggctgtcc ccgcgttcgc gacggcgctc ctccatggcg tgctcgccgg cagcgattcg
    32401 gcgcgggttg gcatcacatc gctctactgg atcaccggat cgtccgtgct cttcctcgtg
    32461 atcgtgcgcg ggctcaccgc gcgaccgccc agagtgcgag cctcgcagtc ctcccgccgc
    32521 gcggggacgt tcgatcgagc ggctccgagg agccgtgcga gccttcccgg gtgaaaccgg
    32581 cacaggacgg gccgagccgt cagggcccgt cgccgatcac gcacctgcgt cggtcgaagg
    32641 tcaccacgag atccgggacc ggtcgggcga ccgagacgat cctcctctca ggaagccgga
    32701 tcgggttcga tcgccagggc tgccaccagt gggtcccaga agcagaaggt cccgtcggcg
    32761 gcctgtccgt tgccgacgaa gagtgcgtgg atcgctcgag ccgccggttc gtcgaggtgt
    32821 cggccccgtg catcacgaag aacggggtcg cgggacatac atgcccgatc tactgcgacg
    32881 ccggtgggct cgtggcgacg gcgtcaaccc atcggaggag gtcgcgctcg gtcacgatcc
    32941 ccaccagctg accatcgtcg atgacgggga gtcgtcggat ccccttctca gtcatgagat
    33001 ggaagatgaa caccacctcg tcggaggggc tcaccgtcgt gacaggggtc gacatgacct
    33061 cgccgatctc acgatcgagg agatccctgt catcgagcaa acccttggtc aagtcgcgct
    33121 cggtgaagac gccgaccgcc gacgctccgt cgacgacgac cacggcgccg atgttgcgat
    33181 cgatcatggc gcggagcgcc tgcgcgaccg tggcatgggg agccacatga accaccggac
    33241 tgctcatgat ccctttcacg tgatcttctt ccagtgacat actcgccacc tttcggtcca
    33301 cggtcgggtg catcatgcca cccggtccga ttcccgcgct cggtcctctc agggagacag
    33361 gcggggtcga ccggggctga cgggtatcgc ggcgacgaag cgcagggggt ccacaccgag
    33421 gcacaccgca tccgccatgc gctggacccc atcgggcccg gcgtggagga cctgcggagc
    33481 cagcatctcc cgtcgcttga tctctcgagg atccacgaag ccgagcgcga tgaccaagcc
    33541 tcgttgcgcc ccgctccgga cgatgcggag gtaggcgtgg ttcagagctt cgtgcccgat
    33601 gacgacgtcg ccggaagcac gcaggatcac cagggccgac tcacccaccc gtatcagatc
    33661 accgtcgatc tcgcagggaa ctctcgcggc ctccaggatg actcgaacga gatcgatcgg
    33721 gtcatccggg tcaccgatcc cgttgcgaac gacctcagga aggtcgaact gatccatgag
    33781 ggagcggtga ggtggcgcga gctcgcccgg ggtgtgtcgg tcgcgatccg gtctcggcgt
    33841 cggttcggat ccgtagaggc cgcccaacac gtcgggaacg tgcgtccgcc ggacgtgcgg
    33901 ctcgtgcagc atcggggaga ccgtgagctc gcgcggcatc ggtgtctccc tgctcatccg
    33961 acccaggatc gtcccggctc gcgtccgaac cacgacttca cgggcatcgg gagcagtcct
    34021 gaagatcccg agaaccgtct cgtggaccag gcgccgtacg gcgggggaac ctggatcggc
    34081 ttcgtcgatc tcgatcgtca cggcgacgac gtgcgatgca ctcaccgtca ggggcgagcc
    34141 ggtcgggggc gcggccgacc gcctggacga tctgagcgcg atgagcgcca ccagcacaga
    34201 ggtcagaa
//
LOCUS       DASUYD010000155        24128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_525798, whole genome shotgun sequence.
ACCESSION   DASUYD010000155 DASUYD010000000
VERSION     DASUYD010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 24128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24128
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525798"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VF195_08725"
     CDS             complement(<1..651)
                     /locus_tag="VF195_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX6580941.1"
                     /translation="MPEDLPRGGRSSRLVDSAIYVDGERVASPASLPETLETLRRHPE
                     GMAWIGLYRPEEREVQSLASEFQLHPLAVEDAINAHQRPKIERHDDTLFVVLRPARYL
                     DDIEEVEFGEVHVFVGPRFVLTVRHSEAPEFGSVRDRLEREPDVLRRGPEAVLYAILD
                     KVVDGYYPVVAGVHHDIDEIEVEVFSGDPKVSRRIYELSREVADFLRATRPLIDVIT"
     gene            complement(878..1273)
                     /locus_tag="VF195_08730"
     CDS             complement(878..1273)
                     /locus_tag="VF195_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580942.1"
                     /translation="MKKMKGQLNVKTRAEHIAAVDDERRSDIAALDAVIRKHAPKLEP
                     VIRGGMLGYGPFRHRYASGREGDACRLDRQQRLVYLAVLLRRRREGLPRRAVRRPAPE
                     GQHRQGLVRFKKLADLDEKALVARIKETA"
     gene            complement(1434..4388)
                     /locus_tag="VF195_08735"
     CDS             complement(1434..4388)
                     /locus_tag="VF195_08735"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6580943.1"
                     /translation="MTLASGPIVGRVEELGSFERALDELDRGRPGALEVAGEAGIGKT
                     RLLKELAARAEARGHLVLVGAASELEHQLPFSVFVDALDEYVAGLESDRLTVVDEDVQ
                     AELAHVFPSLSGLAGDLEVAPQHERYRAHRAVRELLEQLAAPAPLVLVLDDVHWADAA
                     SVELLNALLRRPPTTSVLIALALRPRQAPERLWASLERADREAALTRIELGGLTLAEA
                     RVLLGEALQATEAAALYEEAGGNPLYLQELARALDRATGMAPVAPELSLAIGVPSAVA
                     AALTEELAPLTRRARAVLEGAAVAGDPFEPELAAAAAATSETDVLDAIDELLQLDLIR
                     QTEVPRRFRFRHPLVRRAVYETAPGGWRLGAHERCADALAARGAAVAARAHHVELSAR
                     QGDLDAVELLRKAGEATARLAPGTAARWFGGALRLLPDAESSGDRALLLLARARSLAA
                     AGHFDDSYEALLEATAVVPEGSSALGTSVATACAGMERFLGRYEESHARLEKTLRAQP
                     EPASVESVGLLIELTLNEFYRSRFEAMHEWAGRAVTAAKVLEDPALLAAALAMPALAE
                     AMTGPTGTARAHRAEAAALVDGLSDEELSLRLDGAGWLAAAELYLDLYAEADAHASRA
                     YRLARATGRSDPFGLYQILPRVWCVRGKLAEAAELLDGAIEAGRLLGSPPALAGNLFN
                     RSVVALAVGDLDTALATAEEGVELTRDLDEGFVTAWAGVRLASVLLGSGQPERAVELL
                     LGRAGGEELALIPGGWRAYCLELLTRCWLALDRRKEAERAVADAGAAATAMRLPLAAA
                     WADRAAAAVALFEGDSAHAAERALVSAGAAGEVGAPIEAALSRTLAGRAFAQAGQADR
                     AVEELVGAAATFDACGALYYRQAAERELGKLGHRPHRRTGSGKKGRGTESLTERERQV
                     ARLVVDRRTNLQIAAELFLSEKTVESHLRNIFHKADVRSRVELARLVEREEGRDGSRQ
                     GSLIGQRLDSGP"
     gene            4568..4810
                     /locus_tag="VF195_08740"
     CDS             4568..4810
                     /locus_tag="VF195_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580944.1"
                     /translation="MSRYLLHHRHDPDECGVVFTSFKGHESALRHRATLASCRSGGHS
                     IWWTVDAGSERDALRLLPPYVAERTTITSVSEVQIP"
     gene            5007..5636
                     /locus_tag="VF195_08745"
     CDS             5007..5636
                     /locus_tag="VF195_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580945.1"
                     /translation="MSLISVGLLGGGGAALWADLSHRDEAGYVTTDAHTFSTAGSALA
                     TEPIELGDPGVGWLYSTVILGEVRIRIAPADPDSTLFVGVGPSARVEGYLAGVSQTII
                     SDYWTERLEATGGGTPGSAPPTQDFWVAAASGRGPQTLTWDPANGSWTVVVMNADAQA
                     GVNVRTDLGATMPTLMAIAVGSLVVGGLFLIGGVLLIMGAIRRSRARTA"
     gene            5718..6092
                     /locus_tag="VF195_08750"
     CDS             5718..6092
                     /locus_tag="VF195_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580946.1"
                     /translation="MSQFEVRAMLRVRDGRLEGFKHQAAEMMRHTKEKDVGTLAYDWF
                     LSEDGTRCEVREAYVDADALVDHAFHVREARDTMFDRFAYDHKVAFYGESSPRLDELI
                     ERIGVNVTRFTPLQTLETADVH"
     gene            6105..6575
                     /locus_tag="VF195_08755"
     CDS             6105..6575
                     /locus_tag="VF195_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6580947.1"
                     /translation="MQSVKEAATSFLANKRVAVTGVSRRPKDHGSNTVYKRLRDRGYE
                     VFAVNPNADEVEGDPCYQDLKSIPGGVEAVVIGTRPEIAEDTMRECADLGIDHVWMHR
                     GPGAGSVSATATEYGRQHGITVIDGGCPCMFDPTADFGHKIMRLVFAGHVPKHV"
     gene            6636..6962
                     /locus_tag="VF195_08760"
     CDS             6636..6962
                     /locus_tag="VF195_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEX6580948.1"
                     /translation="MLITTTPTVEGRAIGQYDGLVTGEAIMGANVFKDMFASVRDIVG
                     GRSAAYEEELKKAKDLALGEMAQQAEELGSNAVISVDLDYETVGQGSMLMVSASGTAV
                     RLADQA"
     gene            6965..7822
                     /locus_tag="VF195_08765"
     CDS             6965..7822
                     /locus_tag="VF195_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6580949.1"
                     /translation="MQVDTDTGSVSADGCDLYYEVTGRGVPILLIHPSGATASTWGSA
                     IEGLARLGLVIAYDRRGYARSEGELVRSVSRHASDAAAILESLRQAPAVVVGTSSGAA
                     IAVDLAVRRPDLVRAAIAHEFPWRMNRHLPTASQLAVFAKMGSFVLRGRQADAAEVLL
                     RAAYTYRDGGSAWDAFPEEWRQAGRENAKATLRDFVSSIGNHPSAGDLATVRVPVVCS
                     HGSRSPDFMVRLMRSLASAIPTARTEVIEGAAHAAPFDAATNFVRLIGDTIEEGNRWG
                     AERDEHVRS"
     gene            8243..9589
                     /locus_tag="VF195_08770"
     CDS             8243..9589
                     /locus_tag="VF195_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6580950.1"
                     /translation="MGKIETSTPGGERSGWRLRLQRLRLRFRDPALESKYRADRFRHD
                     LGNIRFGFLAGIAIWVAWGLLLRSYMLALSDQRLDVLMRFGVFIPMLIVGLALTYTRF
                     FGRVWEWVAVAIAAATLLFWVFYVSRISTLPAEYGYIGVILITAFTYALLRLRFVLVV
                     LVTVIGIGAYLPYAITAKYIQDVSLVLAMLYLVSFSMLGGLAAYRTERFTRDLFLRER
                     QLDEERNRSDGLLFNMLPGAIVEQLKTSSGGRIAQNLDQVSVFFADAVGSTDEAARSS
                     PEAFADALDALFRHFDMIADRHGLEKIKTIGDAYMAVAGAPTPMADHADAAVRMAVDI
                     LGDAGTIRWPSGNPIVVHGGIATGPAVAGVIGDRRFAYDLWGDAVNLASRLEESAEPG
                     CVLVSETTARGLTDRYDFGPSETLDVKGKGPTTIRVLGARPRSDVPVTAPLVSEPE"
     gene            9709..10515
                     /locus_tag="VF195_08775"
     CDS             9709..10515
                     /locus_tag="VF195_08775"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6580951.1"
                     /translation="MIVVGVSGFFSENQIVAEMYAQVLEHAGYTVERQLDLRSREVSQ
                     NALEAGVIDVKPEYLSSLLLFVDPNAEASSDADDVARQTAQLLRSEGLTLLTPSGAED
                     TNQFVANAETAQRFDLTTVSSLAPVAGQLTFGGPPECPQRPLCLPGLHEVYGILFDDF
                     TPLDAGGPLTVDALQRDAVQVGLLFSTDPRIERNGFVPLADDKHLQNAENLTPVIRSE
                     KLNDEVRSLLDAVSAQLSTETMTELVGQVVIDGQDVATVATAFLASRDLL"
     gene            complement(10800..11369)
                     /locus_tag="VF195_08780"
     CDS             complement(10800..11369)
                     /locus_tag="VF195_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015575782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6580952.1"
                     /translation="MRSVTYSMGVSLDGFIVGPDGGFDWTAPDEEVFRFWIDEIRGVG
                     VHLLGRRLYETMLYWETADQDPSLGDPELEWAALWNPLPKVVFSTTLSAVQGNARLAS
                     GSLAEEIERLRADPGAGEIAIGGATLAAEAAARDLIDEYRAMVYPVLVGGGTHYFPQR
                     ERRVDLELVETRTFSSRFVYLHYRVARQP"
     gene            complement(11467..11895)
                     /locus_tag="VF195_08785"
     CDS             complement(11467..11895)
                     /locus_tag="VF195_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580953.1"
                     /translation="MSFSASPILWVILSVVILGALGSWLWIRRSRTSRGPSARPAVFS
                     LIGDVTETVLPTGWADEQIVVLVGDVRMDLRDRPPGEGAILRVFHLIGDVRLQVAPGT
                     HVITSGITLLGDQRVDVSAGEGPEFEVRTWGLIGDVKVSS"
     gene            12307..13368
                     /locus_tag="VF195_08790"
     CDS             12307..13368
                     /locus_tag="VF195_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580954.1"
                     /translation="MSGRTARWLAWGTWGLVLAQLVLVLVLGALNGGTVTAEEFFFVP
                     LVLLAVMTSGTVGALVASRQPRNPLGWLFLGLSLCVVLGVMGEDYPIYAIRTNPGSLP
                     APEWVSWVSGWTFGVAGATFPLIILLFPSGSVPSRRWRAVPWLLVGATLWAVAWYAVE
                     PRHLSPTPGLDIPNPVAIEGIRGIASPLITVGTVVAILAAIACFVGLILRFRRSKGEE
                     RQQLRWLAYVGLFCLVMFLALFATDLLPSGAQVVEDVLWSLFVASLAVGIPAACGIAI
                     LKFRLYDLDVVIKKTVVFAVLAGFVTLAYVGVVIGIGTVVTGAETGLNLLVFGAIALV
                     ALVLQPLRHWARRLADRLV"
     gene            13393..14427
                     /locus_tag="VF195_08795"
     CDS             13393..14427
                     /locus_tag="VF195_08795"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6580955.1"
                     /translation="MLSQFTERIGETLSVEDVLARMTQLITAGTGADRAQVWLRVGRE
                     LLLEAGSPPREDDERPGLSLEADDEPPEIPEATEVIPVRHRGELLGAIAVSVPATDPL
                     TPEQERMLSELAAQAGLVLRNVGLTAELRVRLEDLQRSRERLVAAQDEERRRIDRNLH
                     DGAQQQLIALAVKARLAQQLADRDTNKSASLLRDIQRDAESALDDLRDLARGIYPPLL
                     ADQGLGAALEAQARKAPIRVTVDVAVDARYPQAVEAAVYFCCLEALNNVAEYANATRA
                     SVRIARDNGQLRFEVADDGEGFDVGSTTHGTGLQGMADRLDALGGRFEVASAPGTGTT
                     ISGTLTLGET"
     gene            complement(14474..14544)
                     /locus_tag="VF195_08800"
     tRNA            complement(14474..14544)
                     /locus_tag="VF195_08800"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14509..14511),aa:Val,seq:tac)
     gene            complement(14692..14922)
                     /locus_tag="VF195_08805"
     CDS             complement(14692..14922)
                     /locus_tag="VF195_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580956.1"
                     /translation="MPAQQERARADGVAAWAAGVGVGLIALMLTWIVGNRIAMVVWDA
                     PIGPTVAFIGAILVGIVVTVVAGVRLVRSGRR"
     gene            complement(14931..15332)
                     /locus_tag="VF195_08810"
     CDS             complement(14931..15332)
                     /locus_tag="VF195_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580957.1"
                     /translation="MTTTDPGGRGASPLDAPEVDALHLDERPDGPGAAAMMAAGIGIF
                     VTGLFTVLSEASVPIHDWLESLDFGRGVGPLAGKTILGTVGFFASWIVLGMAWRTKDI
                     DLRRWFWIALAVGVVGALLLLPQVFQAFAPE"
     gene            complement(15345..15923)
                     /locus_tag="VF195_08815"
     CDS             complement(15345..15923)
                     /locus_tag="VF195_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580958.1"
                     /translation="MFDLSDREFWTVVHGLVLGTIFLLLFGGGLAGLWSLRPAFLTRV
                     GVQERMRRLRIGMWATAVTAWATVITGTWIVYPWYREALAVRGEDDYAGCAGAVLPTG
                     TCSPRDFLKSNVSGDTESWHNFGMEWKEHIAWAAPFLATAVAFIVAYYGARLIKRPWL
                     RAATLVMFVGAFAAAAIGGFFGAFLNKIAPIV"
     gene            complement(16365..16595)
                     /locus_tag="VF195_08820"
     CDS             complement(16365..16595)
                     /locus_tag="VF195_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580959.1"
                     /translation="MVETGATLFVAEGIWQGVVTVKAVGGSDVAFGVDGVDFRELEAQ
                     DLIQPTSGHGYELTNIGRIEYEQRKNAHGPRL"
     gene            complement(16823..16990)
                     /locus_tag="VF195_08825"
     CDS             complement(16823..16990)
                     /locus_tag="VF195_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580960.1"
                     /translation="MEREPMGGGQEGGGQEGGTQGGGQGGGGGSQEGGQDQEREGQEG
                     GTQGAGLGGGA"
     gene            17158..17286
                     /locus_tag="VF195_08830"
     CDS             17158..17286
                     /locus_tag="VF195_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580961.1"
                     /translation="MSSDELPPDERGELRTEVGQSLILVGMMAVTIIAPLLIGLAF"
     gene            complement(17325..17741)
                     /locus_tag="VF195_08835"
     CDS             complement(17325..17741)
                     /locus_tag="VF195_08835"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6580962.1"
                     /translation="MGWRTEPAPGAPRSRSTELRAGPRTPAEARSFLSEAIEDLDVDV
                     DRAAAILMISEVASNAARHGKEPIHISVSAEEEGFRVSVFDRGAGFDPEDLSFDGGLS
                     VEPLAEGGWGLQVVHNLSSEWGVKRPDDGTEVWFRL"
     gene            complement(17824..18444)
                     /locus_tag="VF195_08840"
     CDS             complement(17824..18444)
                     /locus_tag="VF195_08840"
                     /inference="COORDINATES: protein motif:HMM:NF015799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HEX6580963.1"
                     /translation="MEEQSVPRLRVLVTDGPGVRLDEATLTVAGLGHDVIARESSLPE
                     VGRITATENPDVALVIVHEGNSKGLEMIDRLVHEATCPVIAVLDVQDPAFIQEAAKRG
                     IFAYIAHGEDPQEMQSSIDIVLRRFAEYHNLEGAFGRRALTERAKGILMERHAIDEQA
                     AFDMLRDEARRTNRRVVDLAEAVVVSRSMLPVQASHPEVPGENEDP"
     gene            18619..19023
                     /locus_tag="VF195_08845"
     CDS             18619..19023
                     /locus_tag="VF195_08845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6580964.1"
                     /translation="MRTSQALTTRIDARNDVTSIALSGELDMATVPILNDQLTALEQD
                     GSKVILLDLRDLNFIDSSGLHAFVQAYSRSQQNGHRFLLVGASPIAQRLFEITGTEFL
                     LDGAGNGTATRPLHGTVTDGEHEGEVGAEPHV"
     gene            19016..19351
                     /locus_tag="VF195_08850"
     CDS             19016..19351
                     /locus_tag="VF195_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580965.1"
                     /translation="MSDWRDQEATTQTIFREINEWTEEGNDARFGVGPRIDSYLCECS
                     DRRCTESVQVTRLEYELIRAEPLRFLIALNHENPEIDRLVEENERFGTVDMFHGFPVR
                     IARKTYPRR"
     gene            19387..19713
                     /locus_tag="VF195_08855"
     CDS             19387..19713
                     /locus_tag="VF195_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX6580966.1"
                     /translation="MPNFGIVSDSPRMFFLSGELDMATTPLMDTAIKDAVSKGGPITL
                     DFGDLTFVDSSGVGAVLKAVKALPSGCIVLHGVRNPVQKVVDLMGVNRLPNLHVIPCE
                     VPVAAP"
     gene            complement(20029..20502)
                     /locus_tag="VF195_08860"
     CDS             complement(20029..20502)
                     /locus_tag="VF195_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006435569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX6580967.1"
                     /translation="MDKAGLAAPITGPVTLLGVGLGGLFDGIVLHQILQWHHMLTSQG
                     DFPASTVSGLERNTLWDGLFHATTYVLIVVGLFWLWDRARAGGVKSSWKRVLGWMLVG
                     WGLFNLVEGVIDHHILQIHHVRTGPNELAWDIAFLALGAVLFVGGWTLARSDEHW"
     gene            complement(21025..21498)
                     /locus_tag="VF195_08865"
     CDS             complement(21025..21498)
                     /locus_tag="VF195_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6580968.1"
                     /translation="MDVDRPMETLEESIEVEAPVTAAYNQWTQFEEFPRFMEGVESVT
                     QLDDKRVHWVAEVAGKRKEWDAEITRQVPDREIDWVGLGDPDNRGRVVFESLDGDTSK
                     VTMMLDYDPEGIVEEIGDALGLVKRRVRGDMERFKEFIEVRGQETGGWRGQIGAN"
     gene            complement(21498..22085)
                     /locus_tag="VF195_08870"
     CDS             complement(21498..22085)
                     /locus_tag="VF195_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013393555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580969.1"
                     /translation="MERAQLEELLYQSLETEQGGVRIYEKAVGCAQNDDLKKEWGEYL
                     EQTRNHEVIMLRVLDAFGLSPQNETPGRMIIRTKAQTLVDSMDAAKADPAAAQLVAAE
                     CVVEAESKDHMNWELIGEVAAKLTGEEKKTLEEAHSEVEEEEDEHLYHTMGWARELWL
                     ESLGLPAVLPPPEERKHTKSAIGAERAKQARKEMT"
     gene            complement(22802..23329)
                     /locus_tag="VF195_08875"
     CDS             complement(22802..23329)
                     /locus_tag="VF195_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580970.1"
                     /translation="MQRSELEERATRDRDGDPRVIRDRRESRSSTAALTLALGGLVLV
                     VGTFLDWIDGRELALTDPNGFQIPDGRLALGIGVALLLIGILMATNRRVGSWFDADLL
                     GFALSTVALVTVVSLWIYLASDERSPDVGIYVATAGAAFAMVGSLMALLKSASDRATL
                     DSDGDGDIGRNRRLA"
     gene            complement(23525..23716)
                     /locus_tag="VF195_08880"
     CDS             complement(23525..23716)
                     /locus_tag="VF195_08880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580971.1"
                     /translation="MGKVEEQLKELEVGQATQAATLAGAQATQAAAHAGTWSTMLAGA
                     VGLVVGMFLAIALGAARRR"
BASE COUNT         4081 a         8170 c         7795 g         4082 t
ORIGIN      
        1 cgtgatgacg tcgatcaagg ggcgagtggc gcgcaaaaag tccgccactt cccgtgacag
       61 ctcgtagatg cggcgggata ccttcggatc gccgctgaac acctcgacct cgatctcgtc
      121 gatgtcgtga tggacaccgg ccacgaccgg gtagtagccg tcgacgacct tgtcgaggat
      181 cgcgtagagc acggcctcgg gccctcgacg caggacgtcc ggctcccgct caagccggtc
      241 gcgcaccgaa ccgaactccg gcgcctcgct gtgacgcacg gtcaggacga agcgaggacc
      301 gacgaacacg tggacctcgc cgaactcgac ctcctcgatg tcgtccaggt agcgagcggg
      361 ccgaagcacc acgaacaggg tgtcgtcatg acgctcgatc ttcgggcgct gatgagcgtt
      421 gatcgcgtcc tcgacggcga gaggatggag ctggaactcg gaggccagcg actggacctc
      481 ccgctcctcc ggtcggtaca ggccgatcca tgccatcccc tcggggtgcc ggcgaagggt
      541 ctcgagcgtc tccggcagcg aagcgggaga ggcgacccgt tcgccgtcca cgtagatcgc
      601 gctgtcgacg agccgcgagc tccggccgcc ccgaggcagg tcctcgggca ccgataggcc
      661 gtcatcgcgg tcggcctcgc ggcttcggtc gctcgatctc cgacgtacga gggaacggat
      721 cggcgaccgg cggtgctcgg ctgggctcac gcccagagcg taacccggcc acatcggcgc
      781 gctcgttggg gctggacgca ggatgcttcg gctgacggac cgcgagagat gccggtcgca
      841 cgcgcggcgt cgcgcgggct caggcgacga ggcccatcta cgccgtctct ttgatgcgcg
      901 ccacaagcgc cttctcgtcc aggtccgcga gcttcttgaa ccgcaccagg ccttgccgat
      961 gctggccttc gggagccggc cgacgtaccg ctcggcgagg tagcccttcc cgtcggcggc
     1021 gaagcagtac agcgagatat acgaggcgtt gctggcgatc gagcctgcaa gcgtcgccct
     1081 cccggccgct cgcatagcgg tggcgaaacg gcccgtagcc aagcatgccg cccctgatga
     1141 ccggctcgag cttcggcgcg tgcttgcgga tcaccgcatc gagtgccgct atgtcggacc
     1201 ggcgctcgtc gtccaccgcg gcgatgtgct cggcccgggt cttgacgttc agctgtccct
     1261 tcatcttctt catcgcgtcc ctcgggactc gggcgtcacc agaccggcag cgcccgtcgg
     1321 cccttccatc gatggctgtc gcacgacggg gtcctcacct gtgagtaccc aggagcgctc
     1381 gcgcgtcgtg gtgcaagagt ggtgcaggcg gtgccggacc gatccgcccg accctatggg
     1441 cccgagtcaa ggcgctgccc tatcagcgaa ccctgacggc taccatcgcg accttcctca
     1501 cgttcgacca gtcgcgcaag ctcgacgcgc gatcgcacgt cagccttgtg gaagatgttc
     1561 cgtagatggc tctcgaccgt cttctcgctg aggaacagtt cggcggcgat ctgaagattg
     1621 gtcctgcggt cgacgacgag gcgcgccacc tgcctttcgc gttccgtgag cgactcggta
     1681 ccacgtccct tcttgcctga cccagttctc cggtgaggac gatggccgag cttcccgagc
     1741 tctcgctcgg ccgcttgccg gtagtacagc gcgccgcagg cgtcgaacgt agccgccgca
     1801 cccacgagct cctccacggc tcggtctgcc tggccggctt gggcgaacgc tcggcccgcg
     1861 agcgttctcg acagagcggc ctcgatcgga gctccgactt caccagcggc accagccgat
     1921 acgagtgcgc gttcggcggc gtgagcggag tccccctcga acagagccac ggcggccgct
     1981 gctcgatctg cccacgcggc ggcgagtgga agccgcatcg cggttgcggc ggctccggca
     2041 tcggcgactg ctcgctccgc ctccttccgg cgatccagcg caagccagca gcgggtgagc
     2101 agctcgaggc agtacgctcg ccaacccccg gggatgaggg ccaactcctc gccccccgca
     2161 cgtcccagga gcagctcgac ggctcgttcc ggttgcccgg atcccagaag gacgctggcg
     2221 agcctcaccc cagcccaagc cgtgacgaag ccctcgtcga gatccctcgt gagctcgacg
     2281 ccctcctctg ccgtggcgag tgcggtatcg agatcgccga ccgcaagcgc gaccactgat
     2341 cgattgaaga gattcccggc gagagccggc gggctcccga gcaggcggcc cgcctcgatc
     2401 gcaccatcca gaagttcggc ggcctctgcc agcttgccac ggacgcacca gacacggggc
     2461 aagatctgat agaggccgaa gggatcgctt cgcccggtcg cccgagcgag tcggtaggca
     2521 cggctcgcgt gcgcgtccgc ctccgcgtag aggtcgagat agagttcagc cgcggcgagc
     2581 cacccggcac cgtccaggcg aagcgacaac tcctcgtcgg agagcccgtc cacgagggcc
     2641 gcagcttcgg cccggtgggc tcgcgcggtt ccggtgggtc ccgtcatggc ctccgcgagg
     2701 gcgggcatgg cgagcgccgc tgccaacaag gcgggatcct ccagcacctt ggcggcagtc
     2761 acggctcgtc cagcccactc gtgcatcgcc tcgaatctcg accgatagaa ctcgttcagc
     2821 gtgagctcga tcagcagccc gaccgactcg accgaagccg gctcgggctg cgcgcggagg
     2881 gtcttctcga gtcgagcatg ggactcctca tatcgcccca ggaaacgttc catcccggca
     2941 cacgccgtcg cgacgctcgt gccgagggcg ctcgaccctt cggggaccac cgcggtcgcc
     3001 tccaggagcg cctcatagct gtcatcgaag tgtcccgccg ccgccaacga cctcgcacgt
     3061 gcgagcagca gcagagcacg gtctcctgac gactcggcgt ccgggaggag ccgcagcgcc
     3121 ccgccgaacc atcgcgcagc ggtcccgggt gccaaccgcg ccgttgcttc gccagccttg
     3181 cgcaggagtt cgaccgcgtc caggtcgccc tgacgcgctg agagctcgac gtggtgggcg
     3241 cgcgctgcca cggccgcacc tcgtgccgcg agagcatcgg cgcatcgttc gtgcgcgcca
     3301 agccgccagc caccaggcgc ggtctcgtag accgcccgtc gtacgagtgg gtgccggaac
     3361 cggaaccgcc gcggcacctc cgtctggcgg atcagatcga gctgtaacag ttcgtcgatc
     3421 gcgtcgagca cgtccgtctc ggaggtcgcc gctgcagccg cagccagctc aggctcgaac
     3481 ggatcacctg cgaccgccgc gccttcgagc accgcgcgcg cgcgcctggt cagcggcgcc
     3541 agctcctcgg tcagtgcggc ggcgacggcc gacgggacgc cgatcgccag cgagagctcg
     3601 ggggcgaccg gagccatccc agtcgcgcgg tcgagcgccc gtgccaactc ttgaagatag
     3661 agagggttac cccccgcctc ttcgtacaga gccgccgcct cggtcgcctg gagagcttcg
     3721 ccgaggagga cgcgagcctc tgccaacgtc aacccgccca gttcgatccg ggtcagcgcg
     3781 gcctcgcgat ccgcccgctc gagcgatgcc cacagtcgct ccggcgcctg acgcggccgc
     3841 aacgccagag cgatcagcac ggaagtggtc ggcggtcgac gcaacaatgc attcagcagc
     3901 tcgaccgacg ccgcgtcggc ccagtggaca tcgtccagaa cgagcacgag cggcgccggc
     3961 gccgcgagct gctccaggag ctcgcggacg gcccggtggg cccggtaccg ttcgtgctgg
     4021 ggcgccacct caaggtctcc agcgagcccc gacagcgacg ggaacacgtg cgcgagctca
     4081 gcctgtacgt cctcgtcgac gaccgtcagt cgatccgact ccaggcctgc gacgtactcg
     4141 tcgagcgcat cgacgaagac ggagaacggc agctggtgct cgagctccga agctgccccc
     4201 acgagcacga gatgcccgcg agcttcggcg cgagcggcga gctccttcag gagccgcgtc
     4261 ttgccgatcc ccgcttctcc ggccacctcg agcgcgccgg gacgacctcg atccagctcg
     4321 tccagggccc gctcaaagga cccgagttcc tcgacccgtc cgacgatggg cccgctagcc
     4381 agcgtcacga tccgaccctg gttccatcga aactcgctgg ccagggtcgc ccggcccggg
     4441 caaggagggc cgaaaggtcc tgctcccccc tcgcacacgg gccaggtcct tggaggcgaa
     4501 ggaatcaggg tcaggctcag ggtggtcccc cgatgttcgc tcgggtccca ggccgcacgc
     4561 tggtgccatg tctcgctacc tcctccatca ccgtcacgac ccggatgagt gcggcgtggt
     4621 gttcacgtcc ttcaaggggc acgagagcgc gctccggcat cgtgcgacct tggcctcgtg
     4681 tcgctccggc gggcactcga tctggtggac cgtggacgcc gggagcgaac gagacgcact
     4741 ccggttgctg cccccctacg tggccgagcg caccaccatc acgagcgtca gcgaggttca
     4801 gatcccatga gcaccgaggc acggaagggg tggtaccgat gaatgaaaca ccgacgtcaa
     4861 cgacgaccac aaccactggc atcccaccgc aaccaccacc cgcagccgcc ggaggcccag
     4921 cgacgcggcc cgggtcggca gccgctcccc actcgtcggg caggtggacc gcgggccgga
     4981 tcacggcact cgtgatcgga gccctgatga gcctcatctc ggttggcctg ctcggcggcg
     5041 ggggagctgc cctttgggcc gacctgagcc accgcgacga ggccggctat gtcacgaccg
     5101 acgctcacac cttctccacg gccggatcgg ccttggcgac cgaaccgatc gagctcggag
     5161 acccgggggt cggttggctc tactcgaccg tcatcctcgg cgaggtccgg atccggatcg
     5221 cgcccgcgga tccagattcg acgctcttcg tgggggtcgg gccatccgcc cgcgtcgagg
     5281 gctacttagc cggagtgagc cagacgatca tctcggatta ctggacggaa cggctggagg
     5341 cgaccggtgg cggcacgcct ggatccgctc cccctaccca ggacttctgg gtcgcggcgg
     5401 ccagcgggag aggtccccag accctgacat gggatccggc gaacggatcg tggaccgtcg
     5461 tggtgatgaa cgccgacgcg caggcagggg tcaacgtgag gacggatctg ggagccacca
     5521 tgccgacgtt gatggcgatc gctgtcgggt cgttggtggt cggcgggctc ttcctgatcg
     5581 gtggggtgct cctgatcatg ggcgccatcc gccggagccg cgcgagaacc gcatgaggat
     5641 gccgatgtcg aggcggaggt gatggcgagg gcgctgatgc gccaccgccg agacatgagt
     5701 cgagaggagg aacgacggtg agccagttcg aagtacgcgc catgctgagg gttcgcgatg
     5761 gaaggttgga ggggttcaag caccaggcgg ccgagatgat gcggcacacg aaggagaaag
     5821 acgtcgggac actcgcctac gactggttcc tcagtgaaga cgggacgaga tgcgaggtcc
     5881 gcgaagccta cgtggatgcg gatgctctgg tggatcacgc gttccacgtc cgcgaagccc
     5941 gggacaccat gttcgaccgg ttcgcctatg accacaaggt ggccttctac ggcgagtcgt
     6001 caccccggct cgacgagttg atcgaaagga tcggggtgaa tgtcacgcgg ttcacccccc
     6061 tgcagacgct cgagacggcg gacgtccact aggaaggagg agttatgcag agtgtgaagg
     6121 aagccgcaac ctcgttcctg gccaacaagc gagtggcggt cacgggtgtg tcccggaggc
     6181 ccaaggacca cggaagcaac accgtgtaca agcgcctccg cgatcgtggc tatgaggtct
     6241 tcgcggtcaa cccgaacgcg gacgaggtcg aaggggatcc gtgctaccag gatctgaagt
     6301 cgatcccagg cggtgtcgaa gcggtggtga tcggaacccg gccggagatc gccgaggaca
     6361 cgatgcgcga gtgcgccgac ctcgggatcg atcacgtgtg gatgcatcgg gggccgggcg
     6421 caggcagcgt gtcggccacc gccaccgagt acggccggca acacgggatc accgtgatcg
     6481 acggaggctg cccctgcatg ttcgacccta ccgcggactt cggccacaag atcatgcgtc
     6541 tcgtgttcgc cgggcacgta ccgaagcacg tgtgatgggg gcgcgctcga gcggtgcgaa
     6601 ccgttcggcg cgacctcggg agaagggaga acgcgatgtt gatcacgaca acgccgaccg
     6661 tcgagggaag ggcgatcggg cagtacgacg gtctcgtaac gggtgaggcg atcatgggcg
     6721 ccaacgtctt caaagacatg ttcgccagcg tccgcgacat cgtcggcggc cgttcggcgg
     6781 catacgagga ggagttgaag aaggcgaagg acctcgcgct gggcgagatg gctcaacagg
     6841 cggaagagct cggtagcaac gccgtgatct cggtcgacct cgactacgag acggtgggtc
     6901 aaggaagcat gctgatggtg agtgcgagcg gcaccgcggt ccgcctcgcc gaccaggcgt
     6961 agggatgcag gtggataccg ataccggttc cgtgagtgcg gacggatgcg acctgtacta
     7021 cgaggtgacg ggcagaggag tacccatcct gctgatccat ccttcgggag caaccgcatc
     7081 gacgtggggc tcggcgatcg aggggctcgc gcggctcgga ctggtgatcg cctacgaccg
     7141 tcgagggtac gcccggtccg agggcgagct ggtgcgctcc gtctcgagac acgcgtccga
     7201 cgccgcagcg atcttggagt cgctgcgcca ggcaccggcc gtcgtcgttg gcacgagctc
     7261 cggagcggcg atcgccgtcg acctcgcggt gcgtcggccc gatctcgtgc gagcggctat
     7321 cgcccacgag ttcccatggc ggatgaaccg tcatctcccc accgcgtcgc agctcgcggt
     7381 gttcgccaag atgggatcgt tcgttctccg cggtcggcag gccgacgccg ccgaggtctt
     7441 gcttcgcgcc gcctacacct atcgtgacgg tggaagcgcc tgggacgcat ttcccgagga
     7501 gtggcggcag gcgggccggg agaacgcgaa ggcaaccttg cgggacttcg tgagctcgat
     7561 cggcaaccac ccgtcggcgg gtgatctcgc gacggtcagg gttcccgtcg tgtgcagtca
     7621 cggctcgcgg agccccgatt tcatggtccg gttgatgcga tcgctggcct ccgcgatccc
     7681 gacggcgaga acggaggtga tcgaaggagc agcacacgcg gcgcccttcg atgcggcgac
     7741 gaacttcgtg cgactgatcg gcgacacgat cgaagaaggg aacagatggg gggcggaacg
     7801 cgatgaacac gtacgttcgt gagacacaga gttcagggtc ctgggcgacg gcgatgagct
     7861 ggttgcccag ccgagcagga acccgtcgtg gccacgcaag agtcgagtcg aatgagcgtc
     7921 gctccggaga cgccgttgac acgaacgctg gggtggatcg gagcgccacg agcggtgggc
     7981 gctgagggac tcgccccccc aaccttcgtc tcgtgagggc gaccgaaaca tacaggtcag
     8041 ggcatcgacc agcgagaacg ttgtgatgct gagtacctcg tggcgacaca tcggaccctg
     8101 ctgtcgcgcg tctcagcatc gccggcccgt tgacaggacc cagcaagagg ctcgccggtg
     8161 acccttcgct cgcccgggtc tcccctgctg acaagcgggc gcccagcgcc catcatctcc
     8221 ggcgggaggt cggaagaggc agatgggcaa gatcgagaca tcgacacctg gtggagagcg
     8281 atctgggtgg cggcttcggc tgcagcggct gcggctgcgg ttccgcgatc cggccctcga
     8341 gtcgaagtat cgcgccgacc gtttccggca cgatctgggc aacatccgct tcggcttctt
     8401 ggctgggatc gccatctggg tcgcgtgggg cctgctcctg cggtcctaca tgctggccct
     8461 ttccgaccag cggctcgacg tgctcatgcg gttcggcgtc ttcatcccca tgttgatcgt
     8521 cgggctcgcc ctcacgtaca cgcggttctt cgggcgcgtc tgggaatggg tggcggtcgc
     8581 catcgcagcg gcgacgctcc tgttctgggt cttctacgtc tcgcgcatct cgacgttgcc
     8641 tgcggagtac ggttacatcg gcgtcatcct gatcacggcg ttcacctacg cgctgctgcg
     8701 cctgcggttc gtcttggtcg tgctggtcac ggtgatcggc atcggcgcat atcttccgta
     8761 cgccatcacg gctaagtaca tccaggacgt gagtctcgtg ttggcgatgc tctacttggt
     8821 ctcgttcagc atgctgggcg ggctggccgc ctatcggacg gagcggttca ctcgagacct
     8881 gttcctccgc gaacgccagc tcgatgagga acggaatcgg tccgacgggc tcctgttcaa
     8941 catgctcccc ggggccatag tcgagcagct caagacctcg tccggtggac ggatcgccca
     9001 gaacctcgat caggtaagcg tgttcttcgc cgacgccgtc ggatccacgg acgaggccgc
     9061 gcggtcatcc ccagaggcat tcgccgacgc actggatgcg ctcttccgcc atttcgacat
     9121 gatcgcggat cggcacggcc tcgagaagat caagacgatc ggggacgcgt acatggcggt
     9181 cgccggggca ccgaccccca tggccgatca cgccgacgca gccgttcgga tggcggtcga
     9241 catcctcggc gacgccggga cgatcaggtg gccatcgggt aacccgatcg tggtacacgg
     9301 cgggatcgcc acggggccgg cggtcgcggg ggtgatcggg gatcggaggt tcgcgtacga
     9361 cctgtggggc gatgccgtga atctcgcgag ccggctcgag gagagcgcag aacccggttg
     9421 cgtcctggtc tccgagacga ccgccagagg actgaccgat cggtacgact tcgggccctc
     9481 cgagaccctc gacgtgaagg gtaaggggcc cacgacgatc cgggtcctcg gtgcgcgccc
     9541 ccgatcggac gttcccgtca cggcgccact cgtctccgag cccgaatgag acgcctcggt
     9601 cgcggcctct tcagcacagt agccgtcgtg gtcgtgtgca cactcgtggc cgggtgcacc
     9661 gactccggtg ggggcttgtc ggggcttccg tccaccggag gcgatggggt gatcgtcgta
     9721 ggcgtgtcgg ggttcttctc tgagaaccag atcgtcgcgg agatgtacgc gcaggtcctg
     9781 gagcatgctg gctataccgt cgagcgacag ctcgacctac ggtctcgaga ggtctcgcag
     9841 aatgccctcg aagcgggggt catcgacgtg aagcccgagt acctgtcgtc gttgctgctg
     9901 ttcgtcgacc cgaacgcgga ggcgtcctca gatgctgacg atgtcgcccg gcagaccgcc
     9961 cagctgctcc gctccgaagg gctcacgctg ctcacaccat cgggggccga ggacaccaat
    10021 cagttcgtgg cgaacgcgga gacggctcag cggttcgacc tgacaacggt gagctccctc
    10081 gcccccgtgg ccggtcagct gacgttcggg gggccgccgg aatgccccca acggccgctc
    10141 tgcctcccgg gattgcatga ggtctatggg atcctcttcg acgacttcac gccgttggac
    10201 gcaggagggc cgctgaccgt cgacgcgctc caacgcgacg cggtgcaggt cggcctcctg
    10261 ttctccacgg acccgaggat cgaacggaat gggttcgtcc ccctcgcgga cgacaagcac
    10321 ctgcagaacg cggagaacct cacgcctgtg atccgttcgg agaagctgaa cgacgaggtt
    10381 cgaagtttgc tcgatgcggt gagcgctcag ctctccacgg agacgatgac cgaactcgtg
    10441 gggcaggtcg tgatcgacgg gcaagatgtg gccaccgtgg ccacggcatt cctggcctcg
    10501 agagacctgc tgtgacacgc ttgccggccc gggtccccgc cgaggtggtc tgagcagggc
    10561 ttccctggtg ggcgctgaaa gactcgaacc cccggccttc gcctgaggag ggcggagctc
    10621 ggtgcatctc tgcagctcac gcgccatcct cccggtcgcg catcagtcgt gcaccacggc
    10681 gcacccagtt gtccggcctg accctcgcac ggctcgcctt ccatcggtcg gggcggccag
    10741 ctgcggttcc cacggacgtt gttgacatag gcgacagccc cccgctccga tccgggccct
    10801 tagggctggc gcgccacgcg gtagtggagg tagacgaatc tcgagctgaa ggtgcgggtc
    10861 tcgacgagtt cgagatccac ccggcgctca cgctgcggga agtagtgagt gccaccgcca
    10921 accaacaccg ggtagaccat cgcccgatac tcgtcgatca gatcccgcgc ggccgcctcg
    10981 gcggcgagag tcgcaccgcc gatcgcgatc tcgcccgccc ccgggtcggc tcgcagccgc
    11041 tcgatctcct ccgccaggct gccggaggcc aggcgggcat tgccctgcac cgccgacagc
    11101 gtggtggaga acaccacctt ggggagcgga ttccagagcg cggcccactc gagctctggg
    11161 tcgcccagcg atggatcctg atctgcggtc tcccagtaca gcatcgtctc gtacaggcgt
    11221 cgtcccaaca ggtggacgcc gacccctcgt atctcgtcga tccagaagcg aaagacctcc
    11281 tcgtcgggtg ccgtccagtc gaagccgccg tccggcccga cgatgaagcc gtcaagtgag
    11341 acgcccatcg aataggtcac gctgcgcatc agaggtcctc ctcggttacg agtacgacga
    11401 tacgaccgcc ggacgccgca ggactcatca cggctcgcgg gatctcagat cgagcggccc
    11461 tcgcagctag ctgctgacct tgacgtcgcc gatgagtccc caggttcgga cctcgaactc
    11521 ggggccctcg cctgcgctca cgtcgacgcg ctggtccccg aggagcgtga tcccactcgt
    11581 tatcacgtgg gttccgggag cgacctgcag tcgaacatcc ccgatcagat ggaacacgcg
    11641 aaggatggct ccctcgccgg ggggtcgatc gcgtagatcc atgcgcacgt cgccgacgag
    11701 aaccacgatc tgctcatccg cccatccggt gggaaggacg gtctcggtca cgtcgccgat
    11761 gagcgagaac accgctggtc gcgccgaagg gcccctggat gtccgagaac gcctgatcca
    11821 gagccagctc cccagtgccc cgaggatcac gacggagagg atcacccaga ggatcgggct
    11881 ggcggaaaag ctcacgtccg cctgcatgtc gccgagtgtg gtccggcggg cccggtacgt
    11941 caaaagcccc aagcggtctg tcggtccagc cggttcgagt cgcggaagac agatcccccc
    12001 gtgatgagga tgccgacaag cgcaccgtac ccgaggtcgg tgaccgggat ggcgggaccg
    12061 tccgcaggcc tcgaaccatc ccaacacgag agacgtcgca ccgaagaacg cgatgcccag
    12121 catcagcgag acgaaccaca ccctggcgag gaacgcgata cgccgggtgg atgagacgcg
    12181 atgagactgg aagctgagct cctccccgta ataggaagga atcacgccat tggggcgaga
    12241 tgcgcacgcg cagcccttag acgggcagcc ctttctctct ttggccgtct gaggcaccat
    12301 ctccacatgt cagggcgcac cgcccgatgg ctggcctggg ggacgtgggg actggtcctc
    12361 gctcagctgg tgttggtcct cgtgctgggc gccctgaacg ggggcaccgt cactgccgag
    12421 gagttcttct tcgtcccgct cgtgctgctg gcggtgatga cgtccggcac ggtcggtgcc
    12481 ctcgtcgcct cccggcagcc gcggaaccca ttgggctggc tcttcctagg attgtcgcta
    12541 tgcgtggtcc tcggcgtgat gggagaggac tacccgatct acgcgatccg cacgaacccg
    12601 ggctcccttc ctgcgccgga gtgggtgtcg tgggtgagcg ggtggacgtt cggcgtcgcc
    12661 ggagccacct tcccgttgat catcttgctc ttcccctccg ggagcgtgcc gagccgtcgt
    12721 tggcgcgccg tcccctggct cctcgtcggc gcgacgctct gggcggtcgc gtggtatgcg
    12781 gtcgaacctc gccacttgtc cccgacccca gggctcgata ttccgaatcc ggtggccatc
    12841 gaggggatcc gtggcatcgc cagccctctg atcactgtcg gcacggtggt cgcgatcctg
    12901 gccgcgatcg cctgcttcgt ggggctgatc ctccggttcc gccgatccaa aggcgaggag
    12961 cgccaacagc tccgatggct ggcctatgtc ggcctcttct gcctcgtcat gttcctcgcg
    13021 ctgttcgcga ccgacctgct cccgagcggt gcccaagtag tcgaagacgt tctgtggagc
    13081 ctcttcgtcg cgtccctggc ggtcggcatc cccgccgcgt gcggcatcgc gatcctgaag
    13141 ttccggctct acgacctcga cgtcgtgatc aagaagaccg tcgtcttcgc ggtcctcgcc
    13201 ggcttcgtta ccctcgcgta tgtcggcgtc gttatcggca tcgggaccgt cgtgacgggc
    13261 gcggagacgg ggctcaacct cctggtgttc ggggcgatcg ccctcgtcgc cctggtcctg
    13321 caaccgctgc gtcattgggc gcgccgcctc gccgaccggc tcgtctaggg gaagcgagcg
    13381 acgccctacg aggtgctatc gcagttcacg gaacggatcg gggagacgtt gtcggtcgag
    13441 gacgtcctcg cccgcatgac gcagctcatc acggccggaa cgggcgccga ccgagcgcag
    13501 gtctggctgc gcgtcggtcg cgagctcctc ctcgaggccg gttcaccgcc ccgggaggat
    13561 gacgagcggc ccggactgtc tctcgaggcc gacgacgaac cgcccgagat ccccgaggcg
    13621 acggaggtga tccccgtccg tcatagagga gagctcctcg gcgcgatcgc ggtctccgtc
    13681 cccgcgacgg acccgctcac cccggagcag gaacgcatgc tctcggagct ggccgcacag
    13741 gcgggactcg tccttcggaa cgtcgggctc acggcggagc tccgtgtgag gctcgaggat
    13801 ctgcaacgat cgcgggaacg gctcgtcgcc gctcaagacg aggagcgacg gcggatcgac
    13861 cgcaacctcc acgacggagc ccagcagcag ctgatcgcct tggccgtcaa ggcgcgactc
    13921 gcgcaacagc tcgccgatcg ggacaccaac aagagcgcaa gcctgcttcg cgacatccaa
    13981 cgggacgcag aatctgcgct cgatgatctt cgggacctcg cgcgcgggat ctatcctccg
    14041 ctcctcgccg accagggact cggcgcggcc ctcgaggcgc aggcccgcaa ggctccgatc
    14101 cgtgtcacgg tcgatgtcgc cgtcgacgct cggtatcccc aggcggtcga ggccgccgtg
    14161 tacttctgct gcctcgaggc gctgaacaac gtcgcggagt acgcgaacgc cacccgagcg
    14221 agcgttcgga tcgcgcggga caacggccag cttcggttcg aggtggcgga cgatggggag
    14281 ggattcgacg tcggatcaac cacccacgga accggcttgc aaggaatggc cgatcgcctc
    14341 gatgccctag gtgggcgatt cgaggtcgcg agcgcgcctg gaaccggaac cacgatctcc
    14401 gggacgctca cgctgggcga gacgtagacc atcaccagac cgaccatgcg gtcgatgggc
    14461 gggttggaga gtgggcgatg agggactcga acccccgacc ttcgccttgt aagggcgaag
    14521 ggacatcttg catcactgcg ccttgtaagg gcgaagggac atcttgcatc actgatggag
    14581 cgttgtgacg agtcgcacat gcccccgccc gaggccccgt tgcgcggcaa gcgagaggtc
    14641 ccatggcgtc gtagggttcg cgccgatcga gttccgctga aagacccgac gctatcgacg
    14701 gcccgatcgg accagtcgga cccccgcgac caccgtaacg acgatcccga ccaggatcgc
    14761 gccgatgaac gccacggtcg gtccaatcgg cgcatcccac acgaccatcg cgatccggtt
    14821 cccgacgatc cacgtcagca tcagggcgat gaggccgacg ccgaccccgg ccgcccacgc
    14881 cgcgacgccg tccgcacgcg cgcgctcctg ctgcgccggc atcgttgcac ctactccggc
    14941 gcgaacgcct ggaagacctg cgggagcaac aacagcgcgc cgactacccc cacggcgagt
    15001 gcgatccaga accaccgacg gaggtcgata tccttggtcc gccacgccat ccccagcacg
    15061 atccacgacg cgaagaagcc caccgtgccg aggatcgtct tgcccgcgag cggcccgacc
    15121 ccgcgtccga agtccagcga ttcgagccag tcgtggatcg ggacgctcgc ctcggagaga
    15181 acggtgaaca agccagtcac gaagatgccg atccctgcgg ccatcatcgc ggccgcgccg
    15241 ggtccgtccg ggcgctcgtc cagatgcagc gcgtccacct cgggggcgtc gaggggcgag
    15301 gcgcccctgc cccctggatc cgtcgtcgtc atctctcctt cacctcacac gatcggcgcg
    15361 atcttgttca ggaatgcacc gaagaagccc ccgatcgccg cggcggcgaa cgccccgacg
    15421 aacatcacca gcgtcgctgc tcggagccat gggcgcttga tgagcctcgc cccgtagtac
    15481 gcgacgatga acgcgaccgc cgtcgcgagg aagggcgcgg cccacgcgat gtgctccttc
    15541 cactccatcc cgaagttgtg ccacgactcc gtgtcaccgg acacgttcga tttgaggaag
    15601 tcgcggggtg agcaggtccc ggtcggcagc accgctccgg cacagccagc gtagtcgtct
    15661 tcccctcgaa ccgcgagcgc ctcccgatac caggggtaga cgatccaggt gcctgtgatc
    15721 acggttgccc acgcggtgac cgccgtcgcc cacatcccga tccgcaagcg acgcatccgc
    15781 tcctggaccc ccaccctcgt caggaaggcc ggccgcaggc tccagagacc cgcgaggccg
    15841 cccccgaaca agagcaggaa gatcgttccg agcaccaggc cgtgaacgac cgtccagaac
    15901 tcccgatccg ataggtcgaa catcgccacc actccccggg cgtgccatca ctccccggct
    15961 gattgtcctc cgcacgtcgg ttcgaggcaa actggagtct cggtgcctgg ctatggaagg
    16021 gtcgtgatcg agacatctgg ctctgactcg ttcgataggg ttctgagctg caggtttccc
    16081 ggtaggcgct gagggactcg aacccccgac cttcgccttg taagggcgac cgaaacatgc
    16141 aggtcagcca cctgaccagc gcaaaggctg tgactcccag tacctgggac tacctcggag
    16201 tacctgtgtc tcgtgatgca agagtggtgc agagtgggtg ggggacaaga tgcggggcag
    16261 agcgtcgcga ggcgggtacg ggagcgaaga gtggctagtc gttggggccg tcggaggctt
    16321 cgcaaggtag cgcgacttcc tcgaatattc tcgctgaacg cgacctatag ccgtgggccg
    16381 tgcgcgttct ttcgctgctc gtattcgatc cgaccgatgt tcgtgagttc gtacccgtga
    16441 cccgatgtgg gctggatgag gtcctgcgcc tccaactccc ggaaatcgac gccatcgacg
    16501 ccgaacgcca catccgaacc gccgaccgct ttgactgtca cgactccttg ccagatgcct
    16561 tcagcgacga acagggtcgc tcctgtttcg accatcttca tgaacaagtc caactgcggt
    16621 tgggtcaaag aaaccgtgaa ctcaggctag caatatcgtc gaaacggtga gaccagacct
    16681 gcatgcaaga tctgacgccc aacggatgtc tgcaggcgtc gagatcgagc cgaacagatg
    16741 agggaccgga cgggatcatc gcacccgtcc ggccccgacg gtcgaaagca cgctacgacc
    16801 cgagttatgg cgccttcgcg ccctaggcgc cgcctccgag acccgcaccc tgggtgccgc
    16861 cctcctggcc ctcacgctcc tggtcctggc caccctcctg gctgccgccc ccgccgcctt
    16921 ggccgccgcc ctgagtgcca ccctcctggc cgccgccctc ttggccgcca cccatcggct
    16981 cacgctccat gtcctgccct tctcgttcga cctcccttcg ttgcggacgc attacccggc
    17041 gccaatcggc ctgaagccga gcgcgggacg accagatcta ttgagccaaa gggtccagcg
    17101 caggcgagtc gtagaaggtg cgaatgggta cgaggttggc aggaggaact tctccctatg
    17161 agcagcgacg aacttccacc cgatgagcga ggcgagttgc ggaccgaggt cggccagagc
    17221 ttgattctgg tcgggatgat ggccgttacc atcatcgctc cgttgctgat cggactcgcg
    17281 ttctgagttc gacgcgtcgg cctgagatcg atcgtctccc cgatctacag gcggaaccaa
    17341 acctctgtcc cgtcgtcggg tcgcttcacg ccccattcag acgacaggtt gtgaacgacc
    17401 tggaggcccc atccgccttc cgccaagggc tcgacgctga ggcctccatc gaagctgaga
    17461 tcttccggat cgaagcccgc gccgcggtcg aagacggaga cacggaagcc ttcctcctcc
    17521 gctgacacgc tgatgtggat cggttcctta ccgtgccggg cggcgttcga ggcgacctcg
    17581 gaaatcatga ggatggcggc ggccctatcc acgtcgacgt cgaggtcctc gatagcctcc
    17641 gagaggaacg agcgggcctc cgccggcgtc ctaggccccg cacggagctc cgtcgatctg
    17701 gaacgcggcg caccgggggc cggttccgtc ctccatccca tcctacggag gagatacccg
    17761 aggtcttggg ctgcgacact tcccttttga gaggtcgtcc ttggctacag ccggtagagg
    17821 tccctacggg tcctcatttt cccctggtac ttcgggatgc gacgcttgga cggggagcat
    17881 cgagcgactc accaccactg cctcggcgag gtccacgacc ctccggttgg tgcggcgcgc
    17941 ctcgtctcgg agcatgtcga acgctgcctg ttcgtcgatc gcgtgccgtt ccatcaagat
    18001 gcccttggct cgctcggtaa gcgcccttcg gccgaaggca ccttcgaggt tgtggtactc
    18061 ggcgaaccgt cggaggacga tgtcgatcga gctctgcatc tcctgggggt cttccccgtg
    18121 agcgatgtag gcgaagatac ctcgcttggc ggcctcttgg atgaacgcgg gatcctgcac
    18181 atcgaggacg gcgatcaccg gacaggtggc ctcgtgcacg aggcgatcga tcatctccaa
    18241 gcccttgctg ttgccttcgt gcacgatcac gagggccacg tccgggttct cggtcgccgt
    18301 gatccgaccg acctcaggga gggaagactc ccgagcgatg acgtcatggc ccaatccggc
    18361 caccgtgagg gtggcctcat cgaggcgcac tcccggtcca tcggtgacca ggacacgcag
    18421 acgggggacg ctctgttctt ccatagcccg tcccggaagg acccacggat gatagcaagg
    18481 ggctgttcgg gcgagagcga aaggagtaca ctgacgttgc cgagcccaag aaagacggtt
    18541 ccggcaggtc agaggcccca acccacggtc ctcgcacacg gacggcgcgt cgagtcgcgc
    18601 cagcgaggag tgggtcctat gaggaccagc caggcactca ccacacggat cgacgcgcgg
    18661 aacgacgtca cgagcatcgc cttgtcgggc gaactcgata tggccaccgt gccgatcctg
    18721 aacgatcagt tgacagcctt ggagcaagac ggctcgaagg tgatcctgct cgaccttcgg
    18781 gacctgaact tcatcgactc gtcaggcctt catgccttcg tccaggccta ctcgcgctct
    18841 cagcaaaacg ggcaccgttt cctgctcgtg ggcgcgagcc cgatcgctca gcgattgttc
    18901 gagatcacgg gaacggagtt cctcctcgat ggcgcaggga acggcacagc tacgcggccg
    18961 cttcacggga cggtcacgga cggggaacac gaaggtgaag tgggcgctga gccacatgtc
    19021 tgactggagg gatcaggagg cgacgacgca gaccatcttc cgggagatca acgaatggac
    19081 cgaagagggc aacgacgcgc gattcggggt cgggcctcgt atcgattcgt acttgtgtga
    19141 gtgttccgac cgtcgctgca cggagtccgt ccaggtcacg cgcttggagt acgagttgat
    19201 ccgggccgag cctctccggt tcttgatcgc actgaaccat gagaacccgg agatcgatcg
    19261 gctcgtcgag gagaacgagc ggttcggtac cgtcgatatg ttccatggct tccccgtccg
    19321 gatcgcccgc aagacctatc cccgtcgcta gcgatcgttc gagctatatg tcgagaaggg
    19381 cacatcatgc cgaatttcgg gatcgtgtcg gacagtccgc ggatgttctt cctctcgggg
    19441 gaactcgata tggccacgac gccgctcatg gacacggcca tcaaggacgc ggtgtccaag
    19501 ggcggaccca tcaccctgga cttcggggat ctcaccttcg tggattcatc cggggtcggc
    19561 gcagtcctca aggccgtgaa ggctttgccg tcgggatgca tcgtcctgca tggcgtccgc
    19621 aacccggtgc agaaggtcgt tgacctgatg ggcgttaatc ggctgccgaa cctccacgtc
    19681 atcccgtgcg aggttcccgt cgccgcgcca tgacgggtga agactcgctg gctccgggtg
    19741 cctcgccgcc ttcacgctag cggtcgcccc ctccgccgtc ttcgttgaag cggccccgaa
    19801 cacggactca cctgggagga cgatccacca tcgggcttgc tgggttacga agacggtcca
    19861 gatctcgacg atgtccggac tattccctgt gcctgtttgt gcatggtcac gccgagatgc
    19921 aggcgctgac tcaaccaatc agccggctcg cagtgcgctc ttcctcggag gcgatacatc
    19981 gccaccagga aggaaggcca ggccatcgcg acgactcgag cgaaggccct accagtgctc
    20041 gtccgagcgg gccaatgtcc acccgcccac gaaaagcacc gcgccgagcg cgaggaacgc
    20101 gatgtcccac gcaagttcgt tcgggccggt ccgaacgtgg tggatctgga ggatgtggtg
    20161 gtcgatcaca ccctcgacca ggttgaagag accccagccg accagcatcc agccgaggac
    20221 tcgcttccag gacgacttca cgcctccggc acgagcccta tcccacagcc agaagagacc
    20281 cacgacgatg agcacgtacg tcgtggcatg gaacagcccg tcccacaggg tgttccgctc
    20341 caggccgctg accgtggacg ccgggaagtc cccttgagag gtcaacatgt gatgccactg
    20401 gaggatctgg tgaaggacga tgccgtcgaa gaggcctccg agcccgacgc caagcagcgt
    20461 gacgggcccg gtgatcggtg cagctagtcc agccttgtcc atgatcatgc cccgcactgg
    20521 tatcccactc ccccggcagc aatcatccgt gtgagcccga cgaccacact gcccagcagc
    20581 tcagcgttgc tggctgtcgc cttccttcgg tgctggcttg gacgcttcag tcttctcgcg
    20641 tcctgtggcc ttcctgcaag ctggctcccg agcggtcgtc ttgcgagcgg ccatcttccg
    20701 gcggcctgga gccgattcag ggcttacggc cctcgtcatt cctcctctct tgtggcaccc
    20761 gttacgggct cgcgatatcc gagacgacga ccgagcgtca tgttcgggcg ccctcggcgc
    20821 agtcggaggt gcccgtcagg tccccggcct caggacgacc agtaagacgg cgatggcagc
    20881 ggccagcacg gtgacgacga cgaccgtgcg tacgaatggg ctcacttgaa gggactccct
    20941 ctgccatcgg cggtggcatc tcctatcaac agcgcacaga gcgacacact gaccgtcgag
    21001 cgacgaccac cgcagccagt acggctagtt cgcgccgatc tgcccccgcc agccgccagt
    21061 ctcctgaccc cgcacctcga tgaattcctt gaacctctcc atgtcgcctc ggacacggcg
    21121 cttcacgagc ccgagcgcgt ctccgatctc ctcgacgatg ccctcagggt cgtagtcgag
    21181 catcatggtg accttgctcg tgtcaccatc gagcgattcg aagacgaccc gaccacggtt
    21241 gtccggatca cccaggccga cccagtcgat ctcacgatcg ggcacctgcc gggtgatctc
    21301 cgcgtcccat tccttgcgct tcccggccac ctcggcgacc cagtggaccc gcttgtcgtc
    21361 gagctgcgta accgattcca ctccctccat gaagcggggg aactcctcga actgcgtcca
    21421 ctggttgtag gcggcagtca cgggcgcctc gacctcgatc gattcctcca acgtctccat
    21481 cggtcgatcg acgtccatca cgtcatctcc ttccgggcct gcttcgcgcg ctccgcaccg
    21541 atcgcgcttt tcgtgtgctt cctctcttcc gggggaggca ggaccgccgg cagcccgagg
    21601 gactcgagcc agagctcccg agcccacccc atcgtgtggt acaggtgctc gtcctcttcc
    21661 tcctcgacct ccgagtgcgc ttcctccagg gtcttcttct cttcacctgt gagcttggcc
    21721 gccacctctc cgatcagttc ccagttcatg tggtccttgc tctcggcctc gaccacgcac
    21781 tccgcggcga cgagctgcgc ggcggccgga tccgctttcg ctgcatccat ggaatcgacg
    21841 agcgtctgcg ccttcgtccg gatgatcatc cggccggggg tctcgttctg gggactgagc
    21901 ccgaaggcgt ccaagacccg aagcatgatc acctcgtggt tgcgggtctg ctccaggtac
    21961 tcgccccatt ccttcttcag atcgtcgttc tgggcgcatc ccaccgcttt ctcgtagatc
    22021 cgaacgcctc cctgctctgt ttcgagcgac tggtacaaga gctcctccaa ctgtgccctt
    22081 tccatgattc cccttcccta gccgccgcca ccgacgtgat cccatccgga ctacccctac
    22141 ccgtgaggct gcaaaccgat ctttcttcgc tcgattgcga gaggggcccg ggctcctgcg
    22201 gtgtccgggc cctccccatc tggcgcatag gcgaccaggc cactggcgac ctatccggga
    22261 tggttggggg gagcttcttc ctcgggtcgt cggcatcccg acgccactcg gaagctcgca
    22321 ggccagcctt ccggaccatc ctacccagcg gctctggatc ccgaaaccaa gttggggtga
    22381 agtgccgaca acgcgcctgg gcgcgtcaaa ggatcgcttt gaggcagtgt gggactgagg
    22441 atcagactga agtattgccg cattcctatt gggccggagg acaacgctcg gtcttgatcg
    22501 atggcgtcct cgagcgatga cggacggggt ttcgtttgca tggccgtcaa gcggcgacca
    22561 gccgcgtcgt tgccggcgga actccgtccc tgtcatctct aacaaccgct gcgcgatcgc
    22621 tcgcgccaac gagcaggaat cctacggtcg cgaatccggt cggtccgtcc ccccagttcg
    22681 ggcggcgctc gccgaccgcg gactcgtggt cgttgggtac tcagcagtcc gttgacacca
    22741 gagaccgccg ggatctccta ctcccggcgg tctcggtgac aacgctcccg tgcctagaag
    22801 ctcaggcgag gcgtcggttc cttccgatgt cgccatcacc atcgctatcc agcgtggctc
    22861 tgtcggatgc gctcttgagc agcgccatca gcgatcccac catggcgaac gccgccccgg
    22921 cagtggccac gtagatcccg acatctggag agcgctcgtc cgaggcgagg tagatccaga
    22981 ggctgacgac cgtcacgagc gcgaccgtgg agagcgcgaa gccgagcagg tccgcgtcga
    23041 accaactgcc caccctcctg ttggtcgcca tcaggatgcc gatgaggagt agcgcgacac
    23101 cgatgccgag cgcaaggcgg ccatccggga tctggaagcc attcgggtcc gtgagcgcca
    23161 gttcgcgacc gtcgatccag tcgaggaacg ttcccacgac gaggaccagg cctccgagcg
    23221 ccagcgtcag tgctgcggtg ctgctgcggc tctcccgtcg atcccggatc acacgtggat
    23281 caccatcgcg atcgcgcgtt gcgcgctctt cgagttctga tcgttgcatc aaccttccct
    23341 ctcttccgat tggttcacga acttcggtcg gacccacacc caactggccc gcaggccagg
    23401 acctggttgg ggatggaggg cggcacccgt tatcggatgc gttctcccct cttgctgccc
    23461 cggtggaacg ggtaggggaa tccactaccc gttccaccag gtacttccct ggcttgttgt
    23521 tggctcagcg gcgtcgagcg gcgccaaggg cgatcgcgag gaacatgccg acgacaagtc
    23581 cgacggcccc tgcgagcatg gtgctccagg ttcccgcatg cgcagcggcc tgtgtggcct
    23641 gagctcctgc gagcgtggca gcctgagttg cctgcccaac ctccagctcc ttcaactgct
    23701 cctcgacctt ccccatgcca tcagtggtcg ttgacatttc ttccctcccg tgttgttccg
    23761 ctcttcgagc gggcgatgag agagaggaca aggcgtacaa cccttcctct cgtctcgact
    23821 ggtcttcact cagtaccccc acccaaatgg acgcaaaccg aaggacttcg atcgagacgg
    23881 caatcgctga aatctttcgt ggaccgcacc gatgtttgaa atccctggac gggcgacccg
    23941 ccttcgctgg agaggatcgc cggagatcga ctcgtgcagc gaggcgaact agcttccggc
    24001 aggttgttcg gtcacggttc gctcttgccg tgggctgtga ggggagcacc gcacgaccag
    24061 cacaaaggca tccgcgatgg agacgagctg cgatgggtcc ggcgagcctg atcgcgaagc
    24121 atgtggaa
//
LOCUS       DASUYD010000156         2340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_525962, whole genome shotgun sequence.
ACCESSION   DASUYD010000156 DASUYD010000000
VERSION     DASUYD010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525962"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF195_08885"
     CDS             <1..196
                     /locus_tag="VF195_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580972.1"
                     /translation="VERTNRELDRVDVMLEATGEIVGRVGRISGLVEEAVSGPLVKLI
                     SVGVGLRKAASRFGGKKDGS"
     gene            193..420
                     /locus_tag="VF195_08890"
     CDS             193..420
                     /locus_tag="VF195_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580973.1"
                     /translation="MRRLFWLALGLGAGAASAIMATRFVRRQTAKVAPATLAREARGS
                     VLDLAKLVSESLAEGREAMHQKEDELRSAPR"
     gene            505..>2340
                     /gene="alaS"
                     /locus_tag="VF195_08895"
     CDS             505..>2340
                     /gene="alaS"
                     /locus_tag="VF195_08895"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6580974.1"
                     /translation="MDGDRIREGFLRFFEERGHKRVPSSSLIPPPESGLLLTNAGMNQ
                     FIPYFLGHAPAPFVRATTDQRCFRALDIDNVGHTARHLTFFEMLGNFSFGDYFKPEAV
                     AWAYELVTERYGIDTDRLWVTVLEEDTEAEAAWDAVGLPASRIVKRGKFDEHGEFANF
                     WSTHAPGPCGPCSEIFVDRGPRFGPDGGPDVDEERFMEIWNLVFIQDETDDGVNVVGE
                     LPAKNIDTGSGLERVATVLQDVDSVFETDLLRPLLEVAESLAGRTHGRDERDDVSLKV
                     IAEHGRATTFLIADGVQPSNEGRGYVLRRMLRRVVSHARRLGIEKDVMPPLVGRTVEL
                     FGRAHPELVENRAYVEQVAASEEARFAGTLRQGMTLFETAIGRGAESGRLPGEVVFKL
                     HDTFGFPKELTRELASEAGVEIDDERFDALMAEQRERAKRSAKKVPAEEELATIAGEA
                     GKTEFVGYETLASDGRLLALIGPNGRTPVASEGEEVRFVLDRTPFYAESGGQVGDRGI
                     LRAPGGSIDVLDAQFGPGGLIVHTGVVGSGEIRESEEVHGEVDAHRREATARAHTATH
                     IVHWTLRHVLGDHARQAGSLVAPGRLRFDFPHHSAVPRDVLEQAEE"
BASE COUNT          376 a          766 c          840 g          358 t
ORIGIN      
        1 ggtcgagcgg accaaccgcg agctcgaccg cgtggacgtc atgctcgaag cgaccggtga
       61 gatcgtcggt cgggtcgggc ggatctcggg cctggtggag gaggcggtct ccggtccgct
      121 ggtcaagctc atctcggtcg gcgtgggcct ccgcaaggcc gcgagccggt tcggaggcaa
      181 gaaggacggc tcgtgagacg gctgttctgg ctcgcgctcg ggctcggagc cggggccgcg
      241 agcgccatca tggccacgcg cttcgtccgc aggcagaccg cgaaggtcgc gccggcgacc
      301 ctcgcccgcg aagcgcgcgg gagcgtgctg gatctggcca agctcgtctc cgagtcgctg
      361 gccgaaggcc gcgaggcgat gcaccagaag gaggacgagc tccgctcggc accgaggtag
      421 cggtcgtcga cgatcggatg cgccggacgt gcaggctcac cttctgacag gtcgggcgca
      481 ggcacgcggc tagactgtgc cgtcatggac ggcgaccgga tccgcgaggg gttcctgcgc
      541 ttcttcgagg agcgagggca caagcgcgtc ccgtcttcct ctttgatacc cccacccgag
      601 tccggtctgc tcctcacgaa cgccggcatg aaccagttca tcccatactt ccttggccac
      661 gctccggctc cgttcgtccg ggccacgacc gaccagagat gcttccgagc actggacatc
      721 gacaacgtgg gacacaccgc gcgccatttg acgttcttcg agatgctcgg caacttctcg
      781 ttcggtgact acttcaagcc cgaggccgtg gcgtgggcgt acgagctggt gacggagcgg
      841 tacgggatcg acaccgaccg tctgtgggtg accgtcctcg aggaggacac cgaagccgag
      901 gccgcatggg atgcggtcgg actccccgcc tcacgcatcg tcaagcgcgg caagttcgac
      961 gagcacggcg agttcgcgaa cttctggtcg acccatgcgc ccgggccgtg cggtccctgt
     1021 tcggagatct tcgtggatcg gggtcctcgg ttcggtcccg acgggggtcc cgacgtcgac
     1081 gaagagcgat tcatggaaat ctggaacctc gtgttcatcc aggatgagac cgacgacggc
     1141 gtcaacgtcg tgggtgagct cccggcgaag aacatcgata ccggttcggg cctggagcgc
     1201 gtggccaccg tcttgcagga cgtcgacagc gtgttcgaga ccgacctgct ccgtccgctg
     1261 ctcgaagtcg ccgagtcgtt ggccggacgg acccacggtc gggacgagag agacgacgtc
     1321 tcactgaagg tgatcgccga gcacgggcgc gcgacgacct tcctgatcgc cgacggggtt
     1381 cagccgtcga acgagggccg cggctacgtc ctacgtcgga tgcttcgccg cgtcgtctcg
     1441 cacgcccggc gccttgggat cgagaaggac gtgatgcccc cgctggtcgg ccgaaccgtc
     1501 gagcttttcg gccgagccca tcccgagctc gtcgagaacc gcgcgtacgt cgagcaggtc
     1561 gcggcgtcgg aggaggcgcg gttcgccggc accctccgtc aagggatgac gttgttcgag
     1621 accgcgatcg gtaggggcgc agagtccggg cggctcccgg gggaggtcgt gttcaagctc
     1681 cacgacacct tcggcttccc gaaggagctg acgcgcgaac tggccagcga ggccggcgtc
     1741 gagatcgacg acgaacggtt cgatgccctg atggccgaac agcgcgagcg cgccaagcga
     1801 tcggcgaaga aggtgcccgc ggaggaggag ctcgcgacca tcgcgggcga agcgggcaag
     1861 accgagttcg tcggctacga gaccctcgcg tccgacggcc gactgctcgc gctcatcgga
     1921 ccgaacgggc gaacaccggt cgcgagcgag ggtgaggagg tccggttcgt tctggaccgg
     1981 acgccgttct acgccgagtc cggcgggcag gtcggagatc gggggatcct ccgcgctccc
     2041 ggtggatcga tcgacgtgct ggacgcgcag ttcgggccgg gaggcctgat cgtgcacacc
     2101 ggcgtcgtgg ggtccggaga gatccgcgag agcgaggagg tccacggtga agtggacgcc
     2161 catcgacgag aggcgaccgc ccgcgcccac accgcgacgc acatcgtgca ctggacgctc
     2221 cgccacgtgc tcggcgacca cgcgcggcag gcgggctcgc tcgtcgcgcc cgggcgcctg
     2281 cggttcgact tcccgcatca ctcggccgtc ccccgtgacg tcctcgagca ggcggaggag
//
LOCUS       DASUYD010000157        25633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1629087, whole genome shotgun sequence.
ACCESSION   DASUYD010000157 DASUYD010000000
VERSION     DASUYD010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629087"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..399
                     /gene="yajC"
                     /locus_tag="VF195_08900"
     CDS             55..399
                     /gene="yajC"
                     /locus_tag="VF195_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013885286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEX6580975.1"
                     /translation="MLAILAQAQAQPNAIVSFLPLLLIGVVFYFLLIRPQNQRRRAQM
                     EMQSSIEVGDDIVTTAGIYGTITEIDDDFGIVTVEVAPGVEIRLARAAVATRLADDDE
                     FEDEVEGAEPGS"
     gene            474..1193
                     /locus_tag="VF195_08905"
     CDS             474..1193
                     /locus_tag="VF195_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX6580976.1"
                     /translation="MPVEPVPTHPAPTPAGPRDRATVTLDDVQGSEELSLYIASADRV
                     MEAMGYTEHGFRHANLVASIAYQVLSRLDFTEREATLASVAGYLHDVGNSLARDAHGQ
                     TGAILVYQSLRDSVHGADLMPILAAIANHEETEGVAVSPISAAVILADKSDVHRSRVR
                     RSGRIELDVHDRVNHAVERSFLSVDSGPRTVSLELTIDTDISKVMEYFEIFLGRMQLC
                     RHAAELLDARFRLVINGAELA"
     gene            1190..3364
                     /gene="secD"
                     /locus_tag="VF195_08910"
     CDS             1190..3364
                     /gene="secD"
                     /locus_tag="VF195_08910"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX6580977.1"
                     /translation="MRRNRALWASIIFVLVLVVASAAGFLGGSLRPTLGLDLQGGASV
                     ILTAPEGTPQDVMEQALENIRNRVDAFGVGEPDIALSGNTIEVQIPGSADATVRRRTA
                     DLLCLEGEDLIHGCSGSEEDVRDVLESLEVQDQPSEVCVVDGEGEEIECFSSRAQADT
                     FLAGVQVGPKPEPTPSTSATPSPSATASPSAGPSPAASAYCLTRATGERIRCFETRAK
                     AEDAKDALEVTVTERSYCILPAAPEPSPTPSPSPSGSPSASPSASASASPPPSPSSAP
                     ATFADLDLSEAEPLPCEVGSREEAAGALEAIEIVHEEERFCVVSSVGEQLGCSVRRDE
                     AVDLQRQSGQERLLRVIGETARLEERAVLEIVPASDPRPVTCGTQEERDRPPCAHDAL
                     EDEEVVYPGPIGQGQVAKFVLGPTVITGEDFDSASAVINQSQLGTSDWTVNFQLDGDG
                     ADRFAEATTAAVGAPEPTNQIAIVVDRVVISSPTVQSAITGGTGEITGGFAEQEAKDL
                     ATQLNAGALPVELTRQSVRTVSPTLGEESLEQGILAGAGGLVLLFLYLLFYYRLLGVV
                     AWLGMTIWAVLAVALISIAGDQFGYALSLAGVAGLVISLGVTADSYIVFFERLKDEVR
                     SGKSPRAAVQPAFARAYKTIVAADIVTGLAAAVLYLTAVSSVRGFALTLGVATLLDLF
                     VVWFFKRPTVFLIAKNEKLVNLRGFGLTSGVAGEVTRQGAGL"
     gene            3361..4476
                     /gene="secF"
                     /locus_tag="VF195_08915"
     CDS             3361..4476
                     /gene="secF"
                     /locus_tag="VF195_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX6580978.1"
                     /translation="MNVRHALDTYRGHTIPHVPWVERRNRWFALSGLLVAISLAGLLF
                     GDLNFSIDFEGGARITYTFARPVDAEQVQATLEEQGIDGAEVQIVNDEAVSIRTPSLT
                     GEGISESEVIRRELAEQAGIEPVEVNIEDVGPTWGGEISRKAAIGLVIVLAAIMGYIT
                     LRFELKMAVGAIIALIHDVVITIGVYALTGREVTPETVIAVLTILGFSLYDTVVIYDK
                     IKENLETQSLVGRLGSDGTIDLSLNQVLMRSVNTSLVVLLPILALLLFGGETLKDFAF
                     AMFIGTAIGVYSSIFLAAPLLAVLKKREARFRQLEQKLTDRERVVARRATAAPSATPG
                     ASTGEAAAARATARIATTGRPGQKSKRRKPPAKRKRR"
     gene            4481..5002
                     /locus_tag="VF195_08920"
     CDS             4481..5002
                     /locus_tag="VF195_08920"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEX6580979.1"
                     /translation="MEIEQIRALVRDVPDFPEPGIVFKDITPVLAHADAMSTIIDLIV
                     VHFGRGTVDKVVGIEARGFILAAPVAYHFNAGFVPIRKQGKLPWETASEEYELEYGTA
                     TLELHRDAIAQGERVLIVDDVLATGGTAQAAASLVERCGGVIRGISCLIELEFLKGRS
                     KLEGYEVHTFIQY"
     gene            5076..7370
                     /locus_tag="VF195_08925"
     CDS             5076..7370
                     /locus_tag="VF195_08925"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019523768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX6580980.1"
                     /translation="MGSTEQDVPVERVDDEPQPVTPERGRRLPAIPRPRLRRTESGPP
                     NRLAPILKKVRSYNPKADTKLLERAYRFAEDAHDGQFRLSGEPYVEHPIAVTGILADL
                     RLDTTTLTAALLHDTVEDTEVGLGDIEDGFGNEVARIVDGLTKLDKLEFRSGELAQAE
                     NVRKMIVAMAGDIRVLLVKLADRLHNMRTLAPLSEERRRKIANETLEIHAPLANRLGV
                     QELKWELEDLAFKTLNPGPYREIANLVESRRGERQQLLEGVKGALRAKLKELGVKADV
                     EGRPKHLYSIYEKMVIRGKEFNEIHDLVGLRVLVPSLRDCYGALGAVHALWKPIPGRF
                     KDYIAMPKSNMYQSLHTTVVGPKGIPLEIQIRTQDMHRTAVFGIAAHWRYKEGSKSSK
                     EAQDMAWLGQMMDWLKDMADPQEFMEGLRIDLSGGRVFCFTPKGDVQNLPQGATPVDF
                     AYSIHTEVGHRTVGAKVNGKLVPLDYELRTGDTVEILASKAQGEGPSQDWLQFVKTPR
                     ARTKIRQWFSRGRREDAMESGRDAVKTFVRRQDQTFKRLASEETIARVAGDLKYPDLE
                     ALYVAVGEGHVSPQSIVARMSRLVSGTPDEDVTEVPLARPVRIGKPDISKGVVVRGLG
                     DVWVKLGRCCTPVPGDAIVGFVSRGQGVSVHRDTCPNLRTLQRREPERIIDVDWAEGK
                     PTSFSVAIQVEALDRTRLLSDVATVLSDSHVNILAATSSTGRDRITRLRFTFEMADVT
                     HLSSLLAAVKRVESVYDAYRVVPS"
     gene            7641..7970
                     /locus_tag="VF195_08930"
     CDS             7641..7970
                     /locus_tag="VF195_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580981.1"
                     /translation="MQAVDLPPADLVLASFSLPFCPPEAFPDLWVRIRGSLRPDGRFA
                     GELFGDRDTWASDPEMTFHDVDAARALFHGLELESFVEEEEDGEAFDGPKHWHVFHVI
                     VKRTDAG"
     gene            7967..8422
                     /gene="dtd"
                     /locus_tag="VF195_08935"
     CDS             7967..8422
                     /gene="dtd"
                     /locus_tag="VF195_08935"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015346723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX6580982.1"
                     /translation="MRVVLQRVLRASVVVDGERVSEIGRGYLLLVGVGRGDPADEPGR
                     LAEKIVNLRLFADDAGKTNLSLSEVHGEALVISQFTLYADLRKGRRPSWGHAEDPVVA
                     SERVEALAAALEARAVRVGRGVFGATMEVGLLNDGPFTLVLDGASLSSA"
     gene            complement(8409..8813)
                     /locus_tag="VF195_08940"
     CDS             complement(8409..8813)
                     /locus_tag="VF195_08940"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF393 domain-containing protein"
                     /protein_id="HEX6580983.1"
                     /translation="MEGHVVIYDSDCGFCRWALDRLLRWDRRGRLRATPIQSEEGDRL
                     LADLSEEDRLASWHLVTPDGRRYSGGVAAGPLARLLPAGAPVAILAETFPRTTDRVYR
                     WVARNRDSLGRRLGEEACAVDPSRRPGRSTPN"
     gene            8888..9508
                     /locus_tag="VF195_08945"
     CDS             8888..9508
                     /locus_tag="VF195_08945"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX6580984.1"
                     /translation="MYQDVFDRNPFGTNCWLLGADGREDAVLVDPGFEPDELRAVLER
                     AGRRPAAILLTHAHLDHAYAAGVLAGDDLPVFVHEADAVAFDDIDAWNPGFDNPLEPV
                     KDLRRVVDGDELSFAGMSIGVRHTPGHTPGHCCFVTDATVVAGDLVFAGSIGRSDFPN
                     SSPQAMRASLRWFLSRDDADDVLPGHGPRTTVGRERSSNPFLVNVA"
     gene            9508..10794
                     /gene="hisS"
                     /locus_tag="VF195_08950"
     CDS             9508..10794
                     /gene="hisS"
                     /locus_tag="VF195_08950"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX6580985.1"
                     /translation="MDLRPARGTLDLLPPQGSRMRALYDRAATLARLYGYRYVETPGF
                     EATELFSATSGETSDVVTKEMYTFDDRKGRSLTLRPEGTAPVMRAYLGAMQEQPSRFK
                     AYYLTRMYRYARPQEGRYREHRQFGIEVFGSEAPGADAEVIAVGDAFLRGLGLARIEL
                     QLNSIGDEVCRPAYRDELVVYLRANRDRLNDEHRDRFEDNPLRVLDCKDEACRAVAQG
                     APRITDRLCDPCRAHFDGVRSGLKDAGLSWTLEPTLVRGLDYYTRTAFEFVSPGLSPQ
                     QATLFGGGRYDGLAEVLGGPRVAGVGFGMGLERVLLALEQEGIEAPEEPGLAVFVVGV
                     GDAGHGRAHDLARELRAVGVAADVAFEDRPLKAQLKMADRTGATYAAIVGERELEAGV
                     VTLRRLSDGDQKEIAAGSLAEAIAPTPRDGPVGGTA"
     gene            10791..12596
                     /gene="aspS"
                     /locus_tag="VF195_08955"
     CDS             10791..12596
                     /gene="aspS"
                     /locus_tag="VF195_08955"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX6580986.1"
                     /translation="MKESIYRTAMRTNACGELTAEHADAEVTLCGWVASRRDHGGVTF
                     IDLRDREGIVQVVFHPEDAADAHTAAQRLSAEDVVQVTGRVRTRPPGMANPQLPTGEV
                     EVAAASLEILSEAETPPFPVEDRIEAGEELRLRYRYLDLRRHEMTEALRIRHRINAIT
                     RDHMDSLGFLEVETPLLTRSTPEGARDFLVPSRLWPGSFYALPQSPQQLKQLLMVAGQ
                     DRYYQIVRCLRDEAPRADRSFEFTQLDVEMSFVDQEDVFAVIEPLYARYVLDIHGVEV
                     PTPFPRMTYDEMLGRFGSDKPDLRYGLELADLRTVFAGTGFNAFASVLATEDGAIKGL
                     AAPGGGQLSRKELDQLVQDAKGRGAAGLVWIVVEGDGVRSPVEKHLSKDELDDVLNET
                     GASEGDLVCIVADQADRVHVALDGARRHLADRLGLIPEGEWRYIWYTDPPLFDWSDEE
                     GKWVSDHHPFTAPASDDLDPTTARSKGYDLVLNGYEVGGGSIRIHRPEVQEQVFAVLG
                     LTEAEIEEKFGHLIRAFRYGAPPHGGIAMGLDRIVMLMAGKETLREVTAFPKAQSGAD
                     PLTGAPAPVDVAQLREVGLQVLAPTKEQRARAGEE"
     gene            12599..13000
                     /locus_tag="VF195_08960"
     CDS             12599..13000
                     /locus_tag="VF195_08960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6580987.1"
                     /translation="METLHADDVELLTGKNFAHLAVVRPDGTPHVTVTWIDAAGGTVL
                     VNSAVGRVKDRYLRIDPRVSVTVHDEADPYRWLRVDGVVEGFVTGPEAEEHIDTLHQR
                     YHDGERWTFKEGQERVIYRIRPDRILRRYDG"
     gene            13184..14533
                     /locus_tag="VF195_08965"
     CDS             13184..14533
                     /locus_tag="VF195_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX6580988.1"
                     /translation="MRPRTFQEFVGHHHLLGEGKPLTKLIDGGHLPSTILWGPPGTGK
                     TTLAHLLAVAVGADFQQLSAVSSGVSDARRVMDHSKTTLFRTVLFVDEVHRWNKAQQE
                     ILLPAIEDGSITLIGATTENPYHSLVGPLLSRCLLLRLEPLEPKEIRGLLELAVIDAE
                     RGMGALGVTLTDDALDHLVELSAGDARAALTALEAAVLLADAGETLIDGARVADAVQK
                     RIVAYDRGDSHFDVVSAFIKSMRGSDPDAALFWLGRMIHAGEDPRYIVRRMMIFASED
                     VGLADPLALQVAVAAAHALEHVGMPEARFNLAHAALYLARAPKSDSVTEALDKALSDA
                     DSTDPVPPHLRDAHYAGAAKLGHGEGYEYPHEFEGHHVEQQYRPERFEDEVYYESTGQ
                     GRDVEVEPGTGVPLIDHTVQDPAPQDPAPEESAPQEPAPEEDPAPKRTRHPKRNRPQ"
     gene            14604..15035
                     /locus_tag="VF195_08970"
     CDS             14604..15035
                     /locus_tag="VF195_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEX6580989.1"
                     /translation="MTLLALTGGETALIVLAAFWGLLVLFLCLVLLNTFRVLEATRLT
                     VDAMREETVPLLREIKGSVERTNGHLDRVGGVLDSVGGIVGRVERFTGLLEHAASSPI
                     VKFLSVGAGVKKAAESFGRRSGQRTGKKRSKGSAAPGAPAP"
     gene            15037..15306
                     /locus_tag="VF195_08975"
     CDS             15037..15306
                     /locus_tag="VF195_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580990.1"
                     /translation="MRRSFWVALGLGAGATGAVVASRWARRQANKVKPATLAREARGG
                     LMDLSKLVAESIEEGKRAMEERERELRAELDSSKRGPDPDEPTAA"
     gene            15351..17987
                     /gene="alaS"
                     /locus_tag="VF195_08980"
     CDS             15351..17987
                     /gene="alaS"
                     /locus_tag="VF195_08980"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX6580991.1"
                     /translation="MEGERIRELFLSFFEERGHKRVPSSSLIPPPESGLLLANAGMNQ
                     FIPIFLGQAEPSYRRAVTVQKVMRTNDIENVGTDARHLTFFEMLGNFSFGDYFKADAI
                     AWAHQLVTEGFGIDHDRLWVTVYEEDDEAVQGWVDGVGLSPDRIVRRGMTDAEGELAN
                     YWHTHAAGPGGPCSEIFVDRGAKYGPEGGPDVDEERFMEIWNLVFIQDRIDERLRFVE
                     PLPAKNVDTGSSLERVAVVLQNVDSVFETDLFRPLLEVAESLSGRTHGQDPHDDKSLK
                     IIAEHGRATAFLIADGVQPSTEGRGYVLRRMLRRMVSHARALGIERAVMDPIIDVVVE
                     RFGDAYPSLRDNEAFVRQVAGSEEERFAGTLRQGLVLFDDAKGRARDGRLKGDDAFKL
                     SDTFGFPLQLTEELATEAGLSVDVDRFAALLEEQRDRARRAAKKVPIGLDAGAAPPTT
                     FVGYETLESDATIVTMLDPESRELPVAEEGQDVRIFLDRTPFYAEGGGQVGDRGLIRT
                     SSGVIRVVDTVPAGDRSILHTGVVGSGEVRASTEAHAEVDADRREATARAHTSTHVVH
                     ATLRELLGDHARQAGSLVEPGRLRFDFPHPSAVSSEQLDEAELLANRRLTADDVPRDF
                     ETSMEEAERLGAVMLFGEKYGDVVRVVEIGDYSRELCGGTHVPRTGHIGVIRFLHEGS
                     IGAGMRRVEALVGPDALREINAERALLHDLVEAIGSTDPRSAIDHARKVIEENKRLRS
                     ALGALRKGDRDAVIAALVDGVLEVDGLGLVVSEVAGEDASGLRELAQRIRDRFQGRAA
                     AVVLGGGVDGKAQLVAASTPEAVAKGVTAPELLRDAATVIGGGAGGKDILAMAGGRDG
                     AAVGRALGGIPARVRELLAATS"
     gene            17984..18415
                     /gene="ruvX"
                     /locus_tag="VF195_08985"
     CDS             17984..18415
                     /gene="ruvX"
                     /locus_tag="VF195_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010838865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX6580992.1"
                     /translation="MTAPGPVLGLDPGDARIGVAISDPDRRVAVPVGTVHVGRPPGEL
                     LAIAELVRERGASLIVVGLPLSLRGEHGTRAGHADAFADALADVVAVPIELQDERLST
                     VEAERALAAAGIPGRGRRRVVDASAATVILQAWLDARGHNG"
     gene            18444..19508
                     /gene="mltG"
                     /locus_tag="VF195_08990"
     CDS             18444..19508
                     /gene="mltG"
                     /locus_tag="VF195_08990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX6580993.1"
                     /translation="MSADPSLRRVGRGSRPRRLSLVVALLLFLGVVGAGAWALNHYRT
                     CQEPPPGPRRAVTFTVEEDTPARQVMEGLHDEGVIACGGFVGSLLLRGTGKADQIRAG
                     SFELTTGMTLDAAIDVLTTPPPKVPTVELVIPEGFRLTQIAEQVQDDLGIPAKRFLKE
                     VESGRYVLPPYLPAGSATPEGFLFPKSYEFIEEGLSARVVAEELLEQFGTEADALPFD
                     RTEQLGVSPYELVIIASMIEEEARVDRDRRLIAGVIYNRLRIGMALGIDATLLYDDPT
                     PDGELSSDDLAHDTPYNTRINGGLPPTPIASPGEKSLQAALDPAETDFLYYVLCGADG
                     HHKFALTLGEHNRNVDACLG"
     gene            19492..20355
                     /gene="aroE"
                     /locus_tag="VF195_08995"
     CDS             19492..20355
                     /gene="aroE"
                     /locus_tag="VF195_08995"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEX6580994.1"
                     /translation="MRVSAEGGIGGATHVAGIVGWPVEHSLSPAIHNAAFAAAGLDWA
                     YVPLPVPPEALSEALAGLAPLGLRGVNVTMPHKTETAELLEELSEDARLLRAVNTILV
                     RGDVLEGHNTDAPGFGRFLRRDAGFDPAGAEALMYGAGGAARACALALARAGVGRLTV
                     AVRDEARAAALVSIVESVGVPLVVVRPRDVEGSVADLVVNATPVGAPGEEPAHPALPR
                     LGPGVLVVDLLYDPPTTDLQRRAREAGATAVGGLGHLLEQAALSFELWTGTPAPLDVM
                     SAAALAVLADR"
     gene            20407..21105
                     /locus_tag="VF195_09000"
     CDS             20407..21105
                     /locus_tag="VF195_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6580995.1"
                     /translation="MSDYSALAQRVSLSIAGIRGCLMLSRDGSILGAHPEGEPEADLR
                     TAWVRFAGVGDPDRSYVEFPDQIWAFVRRGSYAAFAVAQVGVRPGVLVDTLEQALLSA
                     EQERVVDREAMRLPEALSAPSGKPRTSLHMADRQPTAEPEPAIGRAEAADGASAAESP
                     RADEVASTPPPDVADPPAAEPDPPAAEPDPPADDVPEAQEGKEEETEVDRILLAKEFA
                     GLLQVPREDDEASR"
     gene            21156..22862
                     /locus_tag="VF195_09005"
     CDS             21156..22862
                     /locus_tag="VF195_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6580996.1"
                     /translation="MTTVKRKSKQLGQILIELGLITSEQLDAALAEHQKTPKALGRVL
                     IDLGMIKEADLVRALAEQVGLEFVDLDDTQIDPASTALLPEALARRYRALPIGERDGK
                     LLVAMSDPANVYALDDIRTITGREVQPVVATSADVEQAIQKFAGLDSQVEQLAAEAAE
                     GSEGEELDLEAALEDAPIVKLVNAIMTQAVAERASDVHIEPGERDVRIRFRVDGVLHE
                     PMRPQPKNLQGGLISRLKVMADLNIAEKRVPQDGRISMKVGTRQLDMRVATLPTVHGE
                     KVVIRVLDKSNAMLDLADLGFLEDSYHRFATAFSKPYGAILVTGPTGSGKSTTMYSTL
                     NILNEESKNIITVEDPVEYRMAGVNQIQVNPKAGLTFASALRSILRADPDIVLIGEIR
                     DKETATIAIEAALTGHLVLSSLHTNDAPSAISRLVEMDVETFLVASSIDAVVAQRLAR
                     ILCDKCKQPYAPDVAELQAAGYAEDSWADVGEVFRAPDNRIAKNDCKACSGTGYRGRI
                     GLYEVMLRSEELERLTVERASADAIRTVAIQQGMTTLREDGLAKVRAGITSIEEVARV
                     VK"
     gene            22864..24048
                     /locus_tag="VF195_09010"
     CDS             22864..24048
                     /locus_tag="VF195_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEX6580997.1"
                     /translation="MSSVVQQDEDVQIPVPELLGALLEKGGSDLHLTAGSPPMVRVHG
                     ELEPAGEYPSLTPRALQGMVYAILPQKLRERFEQELELDMSYSLPGRSRFRVNVFQQR
                     DAVGAVFRVIPFEIKNMDDLGIPNVVMDLARFQRGFVVVTGPTGSGKSTTLAAMVDIV
                     NRERAGHIMTVEDPIEFLHRHKSCVVNQREVGADTHGFAQALKHVLRQDPDVILVGEM
                     RDLETIGTAITAAETGHLVFATLHTQDAPQTIDRIIDVFPPHQQQQVRVQLSTTLQGV
                     VTQQLVPTTDGLGRVAAVEVLVCTPAVRNLIREGKTHQIYSSMQAGGRYGMQTMDMSL
                     AQHIKAGRVSQQVAFERCHDPEELQRLLGGSFTSGPSDGYDSPAAFGSSYEGSGGMLM
                     GG"
     gene            24050..25258
                     /locus_tag="VF195_09015"
     CDS             24050..25258
                     /locus_tag="VF195_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6580998.1"
                     /translation="MADTFQYKVRDRSGAVTTGSLIADSEALVLARLREQGFTPLDVK
                     RKKKSIGQIEFGGKKVKLKEVAIFSRQFATMVNSGLPILRAIAILAEQSSNKELARVL
                     NEARLDVEQGSSLSGALAKHDHVFNNLYIAMVRSGETGGSLDTTLIALADMIEREVRL
                     RGKIKSAMTYPVAIVALVILIMSAMLLFVVPQFESIFSQLGGTLPLPTRGLMFMSDAF
                     KSYWYLVAGSAVGARFLFRRYKRTEQGREVVDSIKLRAPVFGTLFHKTALSRFSSTLA
                     MLMKAGVPILQALEIVSDTVNNKVIGKAITEVQLSVRDGESIAKPLTKYPVFPPMVVQ
                     MISVGEETGQVDQMLEKIAQFYDQEVEAAVDALTSLIEPILIAVIGACVGGAVVALYL
                     PMFNIIKLIQ"
BASE COUNT         4085 a         8648 c         9029 g         3871 t
ORIGIN      
        1 ctgccgctct agaggggcga ggtagactcc ctcgtcccct caatggaggt ctgcatgctc
       61 gccatcctcg ctcaagctca ggcgcagccg aacgccatcg tgagcttcct cccgctcttg
      121 ctcatcggtg tcgtcttcta cttcctgctg atccgccctc agaaccagcg ccggcgggca
      181 cagatggaga tgcaatcgtc gatcgaggtt ggcgacgaca tcgtgaccac ggcggggatc
      241 tacggcacga tcaccgagat cgacgacgac ttcggcatcg tgacggtcga ggtcgctccg
      301 ggcgtcgaga tcaggctcgc ccgcgccgcc gtcgcgacgc gcctggccga cgacgacgag
      361 ttcgaggacg aggtggaagg cgccgaaccc ggctcgtgag cgacccggtc cagcctcgcg
      421 atcctcagga ccctgcagcg gcggttcgtg aacccgacga gccggctccc cggatgccgg
      481 tggagccggt ccccacccat ccggcaccca cgcccgccgg accccgggat cgggcaaccg
      541 tcacgctcga cgacgttcag ggcagcgaag agctctcgct ctacatcgcg tccgccgatc
      601 gcgtcatgga ggcgatgggc tacaccgagc acgggttccg tcacgcgaac ctcgtcgcga
      661 gtatcgccta ccaggtgctg agccgcctcg acttcacgga gcgggaagct acgctcgcat
      721 cggtcgccgg gtacctgcac gacgtgggga attcgctcgc ccgtgacgcg cacgggcaga
      781 ccggcgccat cctcgtctac caatcgctcc gcgactcggt gcacggcgcg gatctgatgc
      841 cgatcctcgc cgcgatcgcg aaccacgagg agaccgaagg ggtcgccgtg tcgccgatct
      901 ccgccgccgt gatcctcgcc gacaagtcgg atgttcacag gagccgcgtg cgcaggtcgg
      961 gtcggatcga gttggacgtt cacgatcgcg tgaaccatgc cgtcgagcgt tccttcctct
     1021 ccgtagactc cggaccccgc accgtcagcc tcgagctgac gatcgacacc gacatcagca
     1081 aggtcatgga gtacttcgag atcttcctcg gacgcatgca gctctgccgt cacgccgcgg
     1141 agctgctcga tgcccgtttc cgactggtga tcaacggggc agagctggcg tgagacgcaa
     1201 tcgcgccctc tgggcgtcca tcatcttcgt cctggtcctc gtggttgcct ccgcagcggg
     1261 attcctcggc gggagcctgc gcccgacact cgggctcgac ctccagggtg gcgcctccgt
     1321 gatcctgacc gcccccgaag gcacgccgca ggacgtcatg gaacaggcgc tcgagaacat
     1381 ccggaaccgg gtggacgcgt tcggggtggg cgaacccgac atcgcgctgt cggggaacac
     1441 gatcgaggtg cagatcccgg gaagcgccga cgcgacggtg cgccgtcgaa cggccgacct
     1501 cctctgcctg gaaggcgagg acctgatcca cgggtgctcc gggtccgagg aggacgtccg
     1561 agacgtcctc gagagcctgg aggtccagga ccagccgtcc gaggtctgcg tcgtcgacgg
     1621 ggagggcgag gagatcgagt gcttctcgag ccgggcacag gcagacacgt tcctcgcggg
     1681 cgtccaggtc ggaccgaagc cggaaccgac gcccagcacg tccgccaccc cgagtccgag
     1741 cgccacggcg tccccttccg ccggcccctc gcccgctgcg agcgcgtact gcctgacacg
     1801 ggcgaccggc gagcggatcc ggtgcttcga gacccgcgcg aaggccgagg atgcgaagga
     1861 tgcgctcgag gtcacggtca ccgaacgttc gtattgcatc ctcccggccg cacccgagcc
     1921 ttcgccgacc ccgtcgccct cgccgtccgg atctccgagt gcatcgccgt cggcatccgc
     1981 ctccgcgtcg ccccccccat ccccatcctc ggccccggcc acgttcgcgg atctcgatct
     2041 ctcggaggcc gagccgctgc cgtgcgaagt cggatcccgc gaggaggccg ccggggcgct
     2101 cgaggcgatc gagatcgtgc acgaggagga gcggttctgc gtcgtgagct cggtggggga
     2161 gcagctgggg tgctcggtgc gacgggatga agcggtcgac ctccagcgtc agtcgggtca
     2221 ggagcggctg ctccgcgtga tcggggagac ggcgcgcctg gaggaacgag ccgtgctcga
     2281 gatcgtcccg gcctccgacc cgcggcccgt cacctgcggc acgcaggagg agcgcgatcg
     2341 cccaccttgc gctcacgatg ccctggaaga cgaggaggtc gtgtacccgg gaccgatcgg
     2401 gcaggggcag gtggcgaagt tcgtcctcgg cccgaccgtg atcaccgggg aggatttcga
     2461 cagcgcatct gcggtgatca accagagtca gctcggcaca tcggattgga ccgtgaactt
     2521 ccagctggat ggggacggcg ccgaccggtt cgcggaggcg accacggcgg ccgtcggcgc
     2581 gccggaaccg acgaaccaga tcgcgatcgt cgtggatcgg gttgtgatct catcgcccac
     2641 ggtgcagtcc gcgatcaccg gcgggaccgg cgagatcacc ggcgggttcg cggaacagga
     2701 ggccaaggac ctcgcgacgc agctgaacgc gggcgctctc cccgtggagc tgacgaggca
     2761 gtcagtgcgg acggtgagcc cgacactcgg cgaggagtcg ttggagcaag ggatcctcgc
     2821 cggcgcgggt ggcctcgtcc tcttgttcct ctacctgctc ttctactacc ggctcctcgg
     2881 ggtcgtcgcg tggctcggca tgacgatctg ggccgtcctc gccgtcgcgt tgatctcgat
     2941 cgcgggcgac cagttcgggt acgcgctctc gctcgccggc gtggctgggc tcgtgatctc
     3001 gctcggcgtc accgccgact cctacatcgt gttcttcgaa cggctgaaag acgaggtccg
     3061 ctccggcaag tcgcctcgcg ccgcggtcca gcccgccttc gcccgtgcgt acaagacgat
     3121 cgtcgccgcc gacatcgtga cggggctcgc ggccgcggtc ctgtacctga cggcggtcag
     3181 ctccgtccga gggttcgcgc tgacgctggg cgtggcgacc ctgctcgatc tgttcgtcgt
     3241 gtggttcttc aaacggccga cggtcttcct gatcgccaag aacgagaagc tcgtgaacct
     3301 ccgcgggttc ggtctcacca gcggggtcgc cggcgaagtc acgcggcagg gagcggggct
     3361 gtgaacgttc gacacgcgct cgatacctac cgcggtcaca cgatcccgca cgtgccgtgg
     3421 gtggagcgcc gcaaccgctg gttcgcactc tcggggttgc tggtcgccat ctcgctcgcg
     3481 ggactgctct tcggcgatct gaacttctcg atcgacttcg agggaggcgc gcgcatcacg
     3541 tacacgttcg cgcggccggt cgacgccgag caggtgcagg cgacgctcga ggagcagggg
     3601 atcgacggcg ccgaggtcca gatcgtgaac gacgaggcgg tgtcgatccg aaccccttcg
     3661 ctcaccgggg aagggatcag cgaatcggag gtgatccgtc gggagctcgc cgagcaagcg
     3721 ggcatcgagc cggtggaggt gaacatcgag gacgtcggtc cgacgtgggg cggggagatc
     3781 tctcggaagg ccgcgatcgg gctggtgatc gtgctggccg ccatcatggg ctacatcacg
     3841 ctccggttcg agctgaagat ggcggtcggg gcgatcatcg cgctgatcca cgacgtcgtg
     3901 atcacgatcg gcgtgtacgc gctcacgggg cgggaggtga ccccggagac ggtgatcgcg
     3961 gtgctcacga tcctcgggtt ctccctctac gacacggtcg tgatctacga caagatcaag
     4021 gagaacctcg agacgcagtc actggtgggg agactggggt cggacgggac gatcgatctc
     4081 tcactcaacc aggtgctgat gcgatcggtg aacacgtccc tcgtggtcct gctcccgatc
     4141 ctggccctgt tgctcttcgg cggcgagacg ctcaaggact tcgcgttcgc gatgttcatc
     4201 ggtacggcga tcggcgtgta ctcgtcgatc ttcctcgccg cgccgctcct cgcggtgctg
     4261 aagaagcggg aggcgcgatt ccgacaactc gagcagaagc tcacggaccg ggaacgggtg
     4321 gtggcgcggc gggcgacggc cgctccgtcg gcgacccccg gcgcgagcac cggcgaggcg
     4381 gccgccgccc gcgcgaccgc ccggatcgcc acgaccgggc gacccggaca gaagtcgaag
     4441 cggcgcaagc cgccggccaa gcgcaagcgg aggtgacgtc gtggagatcg agcagatcag
     4501 ggcgctggtc agggacgtgc ccgacttccc cgagccgggc atcgtcttca aggacatcac
     4561 gccggtcctg gcgcacgccg acgcgatgtc gacgatcatc gatctgatcg tcgtccattt
     4621 cggccggggc accgtcgaca aggtcgtggg gatcgaggct cgggggttca tcctcgcggc
     4681 cccggtggcg taccacttca acgccggctt cgtgccgatc cgcaagcagg gcaagctccc
     4741 ctgggagacg gcgtccgagg agtacgagct cgagtacgga acggccacgc tcgagctcca
     4801 ccgggacgcg atcgcgcagg gcgagcgcgt cctgatagtc gacgacgtct tggccaccgg
     4861 cgggacggcg caggcggcgg cctcgctcgt ggaacgctgc ggaggggtga tccgcggtat
     4921 ctcgtgcctg atcgagctcg agttcctgaa aggacgctcg aagctcgagg gctacgaggt
     4981 ccacacgttc atccagtact aggcctggca gggatcgacc ccgtgctccc gagctccaca
     5041 gacggcgtcc cctacacttg gtgcaggagg cgagcgtggg cagcaccgag caggacgtac
     5101 cggtcgagcg ggtcgatgac gagccgcagc ccgtcacgcc cgagcgcggc cggcgcctcc
     5161 cggcgatccc gcgcccgcgg ctgcgacgaa ccgagtccgg accgccgaac cgtctcgcgc
     5221 cgatcctgaa gaaggttcgc tcctacaatc ccaaggccga cacgaagctc ctcgaacgcg
     5281 cctatcggtt cgccgaggac gctcatgacg gacagttccg cctctcgggc gagccgtacg
     5341 tcgagcatcc catcgccgtg acggggatcc tcgcggatct ccggctcgac accacgaccc
     5401 tcaccgcggc cctcctccat gacaccgtcg aggacacgga ggtcgggctc ggggacatcg
     5461 aggacgggtt cggcaacgag gtcgcgagga tcgtcgacgg cctcacgaag ctcgacaagc
     5521 tcgagttccg gagcggcgag ctcgcgcagg ccgagaacgt tcgcaagatg atcgtggcga
     5581 tggccggcga catccgggtg ctgctcgtca agctcgccga ccggctgcac aacatgcgga
     5641 cgctcgcgcc actctcggag gagcgccgac gcaagatcgc gaacgagacg ctcgagatcc
     5701 acgcgccgct cgcgaaccgc ctcggggtgc aggagctgaa gtgggagctc gaggacctcg
     5761 cgttcaagac gctcaacccg ggtccctatc gtgagatcgc gaacctcgtc gagagtcgca
     5821 ggggagagcg gcagcaactc ctggagggcg tcaaaggggc cctccgggcg aagctcaaag
     5881 agctcggcgt gaaggcggac gtcgaggggc gcccgaagca cctgtattcc atctacgaga
     5941 agatggtgat ccgcggcaag gagttcaacg agatccacga cctcgtcggt ctccgtgtct
     6001 tggtgccgtc cctgcgcgac tgctacggcg cgctcggtgc cgtccacgcg ctctggaagc
     6061 cgatcccggg ccgtttcaag gactacatcg cgatgccgaa gtcgaacatg taccagtcgt
     6121 tgcatacgac cgtcgtggga ccgaagggca tccccctgga gatccagatc cggacgcagg
     6181 acatgcatcg cacggccgtg ttcggcatcg ccgcgcactg gcgctacaag gaggggagca
     6241 agtcctcgaa ggaagcacag gacatggcct ggctcggcca gatgatggat tggctgaagg
     6301 acatggccga cccgcaggag ttcatggagg gtctgcggat cgacctgtcg ggcgggcggg
     6361 tcttctgctt caccccgaag ggcgacgtgc agaacctgcc acagggcgcg acgccggtgg
     6421 acttcgccta ctcgatccac acggaggtgg gccaccgcac ggtgggggcc aaggtgaacg
     6481 ggaagctcgt cccgctcgac tacgagctcc gcaccgggga taccgtcgag atcctcgcgt
     6541 cgaaggcaca gggcgaggga ccgagccagg actggctcca gttcgtgaag acgccgcggg
     6601 ccaggacgaa gatccgccaa tggttcagcc gcggccgccg cgaggacgcg atggagtccg
     6661 ggcgcgacgc cgtcaagacg ttcgtgcgcc ggcaggatca gaccttcaag cgcctggcgt
     6721 cggaagagac gatcgcgcgc gtcgcagggg atctgaagta cccggacctc gaggccctct
     6781 acgtcgcggt cggggagggg cacgtgtctc cgcagtcgat cgtcgcgcgg atgtcacgcc
     6841 ttgtctccgg gacaccagac gaggacgtga cggaggttcc actcgcccgg cccgtgcgga
     6901 tcgggaagcc cgacatctcg aagggcgtcg tggtgcgagg tctgggggac gtctgggtga
     6961 agctcggacg gtgctgcacc ccggtgccgg gggacgcgat cgttggattc gtctcccgcg
     7021 gccagggcgt ctccgtccac cgcgatacgt gtcccaatct ccggacgctc cagcggcgcg
     7081 agccggagcg gatcatcgac gtggactggg cggagggcaa gcccacgtcc ttctcggtgg
     7141 cgatccaggt ggaggccctc gaccggacgc ggctgctctc cgacgtcgcc accgttctgt
     7201 ccgacagtca cgtcaacatc ctcgccgcca cctccagcac ggggcgggac cgcatcacgc
     7261 gcctccggtt cacgttcgag atggccgatg tgacgcatct ctcctcgctg ctcgcggcgg
     7321 tgaagcgggt ggagagcgtg tacgacgcct accgcgtcgt cccgagctga ggtggcctcc
     7381 ggagactgga ccggctacta cgacgcccag gaaggtcgcg aaccgcggga gctgctgatg
     7441 gaggtgctcc gggcgttcga tgccgaacac cgggtgggga gcgcagtcga tatcgggtgc
     7501 ggcgacggta ccgactcgct ggccctcctc gagcgagggt ggcacgtgct gtcgatcgac
     7561 gcccaggacg acgcgatccg gcgtcttcgc gcgcggctca cggacgaagt ggccgcgcgc
     7621 tcgacgatcg tcactccccg atgcaggccg tcgatctacc cccggccgac ctcgtgctcg
     7681 cgagcttcag cctcccgttc tgcccgccgg aggcgttccc ggacctgtgg gtccggatcc
     7741 gggggtcgct ccgcccggat ggccgcttcg cgggcgagct cttcggcgac cgcgacacgt
     7801 gggcgtccga tcccgagatg acgttccacg acgtcgacgc agctcgtgcg ctgttccacg
     7861 ggctcgagct cgagtccttc gtcgaagagg aggaggacgg cgaggccttc gacggaccga
     7921 agcattggca cgtgttccac gtgatcgtga aacggacaga tgccggatga gggtcgtgct
     7981 gcagcgggtg ctccgcgcgt ccgtggtggt cgacggagag cgagtctcgg agatcgggcg
     8041 cgggtacctg ctgctggtcg gcgtggggcg cggcgatccg gccgatgagc ccgggcggct
     8101 ggcggagaag atcgtcaacc tccggctgtt cgccgacgac gccggcaaga cgaacctgtc
     8161 gctgtcggag gtgcacggcg aggccctcgt catctcgcag ttcacgctct acgccgatct
     8221 gcggaagggt cgacgcccgt cctgggggca cgcggaggat ccggtggtcg catcggagcg
     8281 ggtcgaagcg ctcgccgcgg cgctggaagc ccgcgcggtg cgcgtgggac gcggtgtgtt
     8341 cggtgcgacg atggaggtgg ggctcctcaa cgacggcccg ttcacgctgg tgctggacgg
     8401 cgcgtcgctc agttcggcgt agatcgcccg ggtcgccggc tcggatcgac ggcgcacgcc
     8461 tcttccccga gccgccggcc gagcgagtcc cggttccgcg ccacccaccg gtaaacgcgg
     8521 tcggtggtcc gagggaacgt ctcggccagg atggcgacgg gagcgcctgc cggcaggagc
     8581 cgagcgagcg ggccggccgc cacgccgccg gagtagcgcc gcccgtcggg cgtgacgagg
     8641 tgccaggacg ccagccggtc ctcctcgctc aggtcggcca ggagccgatc gccctcctcg
     8701 ctctggatcg gagtggcccg gagtcggccg cgccggtccc accgcagcag acggtcgagc
     8761 gcccatcggc agaagccaca gtcgctgtcg tagatgacga cgtggccctc catggccaca
     8821 gcgtacggtg ccacgaggcg gagccgatcg gggcggccgc gccggatcga ccggtacgat
     8881 gcgccagatg taccaggacg tcttcgatcg gaacccgttc ggcacgaact gctggctcct
     8941 cggcgccgat ggcagggagg atgccgtact cgtcgacccc ggtttcgagc cggacgagct
     9001 ccgagcggtc ctggagcgcg ccgggcggcg accggcggcg atcctcctca cgcacgcaca
     9061 tctcgaccac gcctacgccg cgggcgtgct cgccggcgac gacctccccg tcttcgtcca
     9121 tgaggcggac gccgtcgcgt tcgacgacat cgatgcctgg aaccccgggt tcgacaaccc
     9181 cctggagccc gtgaaggacc tgcgccgcgt cgtcgacggt gacgagctct cgttcgccgg
     9241 gatgtcgatc ggcgtccgcc acacgccagg acatacgccc ggtcactgct gcttcgtgac
     9301 cgacgccacc gtggtcgccg gcgacctggt gttcgccggt tcgatcggcc gctccgattt
     9361 cccgaactcc tcgccccagg cgatgcgggc gagcctccgt tggttcctgt cgagggacga
     9421 cgccgatgac gtccttccgg ggcacggtcc acggaccacc gtggggcggg agcgctcgtc
     9481 caacccgttc ctggtgaacg tggcctgatg gacctgcgac cggcacgagg cacgctcgac
     9541 ctgctcccgc cgcagggcag ccgcatgcgc gcgctctacg atcgcgcggc gaccctcgct
     9601 cgcctctacg ggtaccgcta tgtggagacg ccgggcttcg aggccaccga gctcttctcc
     9661 gcgacgtcgg gtgagacgag cgacgtcgtc acgaaggaga tgtacacgtt cgacgatcgg
     9721 aaggggcgct cgctgacgct ccgtccggag ggaaccgcgc cggtgatgcg cgcctacctc
     9781 ggcgcgatgc aggagcagcc gtctcggttc aaggcgtact acctcacgcg gatgtatcgg
     9841 tacgcccggc cgcaggaagg ccgctaccgc gagcaccggc agttcggcat cgaggtgttc
     9901 gggagcgagg cgcccggcgc cgacgccgag gtgatcgcgg tgggcgatgc attcctccgc
     9961 gggctcggac tcgcccgcat cgagctgcag ctcaactcga tcggggacga ggtctgccgc
    10021 cccgcgtacc gggacgagct cgtggtgtac ctgcgcgcca accgggaccg cctgaacgac
    10081 gagcatcggg accgcttcga ggacaacccg ctccgcgtcc tcgactgcaa ggacgaggcg
    10141 tgtcgggcgg tggcgcaggg ggccccgcgc atcaccgacc ggctgtgcga cccgtgccgg
    10201 gcgcacttcg atggtgtgcg gtccggtctg aaggacgccg gtctctcgtg gaccctcgaa
    10261 ccgacgctgg tccggggcct cgactactac acgcggacgg cgttcgagtt cgtgagcccg
    10321 ggtctctcgc cgcagcaggc cacgttgttc ggcggcggcc ggtacgacgg tctggccgag
    10381 gtgctgggcg ggccgcgcgt cgccggcgtc gggttcggga tgggtctgga acgcgtcctc
    10441 ctcgcgctcg agcaggaggg gatcgaggca ccggaagagc cgggactcgc cgtgttcgtg
    10501 gtcggggtcg gcgacgccgg ccacgggcgg gcgcacgacc tggctcgcga gctgcgggcg
    10561 gtcggcgtgg cggcggacgt cgcgttcgag gaccgaccct tgaaggccca gctgaagatg
    10621 gccgaccgca ccggtgccac gtacgcggcg atcgtggggg agcgcgagct cgaggccggg
    10681 gtcgtgacgc ttcgccgcct ctcggacggt gatcagaagg agatcgccgc gggatcgctc
    10741 gccgaggcga tcgctccgac gcctcgcgac gggccggtcg gaggaacggc atgaaagaga
    10801 gcatctatcg caccgcgatg cgcaccaacg cgtgcggcga gctgaccgca gagcacgccg
    10861 acgccgaggt gacgctctgc ggctgggtcg cgagccgccg cgaccacggc ggcgtcacgt
    10921 tcatcgacct ccgcgatcgc gagggcatcg tgcaggtcgt cttccatccg gaagacgcgg
    10981 cggacgccca caccgccgcc cagcgactga gcgccgagga cgtcgtgcag gtcaccggcc
    11041 gcgtgcgcac ccgcccgccc gggatggcca acccgcagct ccccaccggc gaggtagagg
    11101 tcgcggcggc ctcattggag atcctatcgg aggccgagac gcccccgttc ccggtcgagg
    11161 atcggatcga agcgggcgag gagctccgct tgcgctaccg ctacctcgac ctccgccggc
    11221 acgagatgac ggaggcgctc cgcatccgcc atcggatcaa cgcgatcacg cgcgatcaca
    11281 tggattccct cggcttcctc gaggtcgaga cgcccctgct cacgcgctcg acgccggagg
    11341 gcgcgcggga cttcctcgtg ccgtcgcgac tctggccggg gtctttctac gcgctcccgc
    11401 aatccccgca gcagctgaag cagttgttga tggtggcggg acaggatcgc tactaccaga
    11461 tcgtccggtg cctccgggac gaggcgccgc gtgcggaccg gagcttcgag ttcacccagc
    11521 tcgacgtgga gatgtcgttc gtcgaccagg aggacgtgtt cgcggtgatc gagcccctgt
    11581 acgcgcgcta cgtgctcgac atccacgggg tcgaggtccc cacgccgttc ccgcggatga
    11641 cctacgacga gatgctgggg cgcttcggca gcgacaagcc ggacctccgc tacggcctgg
    11701 agctcgccga tctcaggacg gtgttcgccg gcacgggctt caacgcgttc gcgtccgtgc
    11761 tcgccacgga ggacggcgcg atcaagggac tcgcggcacc cgggggcggc cagctctctc
    11821 gcaaggagct cgaccagctc gtccaggatg cgaagggccg cggcgccgcc ggtctcgtct
    11881 ggatcgtcgt ggagggcgac ggcgtgcgct ctcccgtgga gaaacacctg tcgaaggacg
    11941 agctcgacga cgtgctcaac gagaccggtg cctccgaggg agacctggtg tgcatcgtgg
    12001 ccgatcaggc cgatcgagtg cacgtcgcgc tcgacggcgc gagacggcac ctggccgatc
    12061 gactcggtct gatccccgag ggagagtggc gttacatctg gtacacggac cccccgctgt
    12121 tcgattggag cgacgaggag gggaagtggg tatccgacca ccaccccttc accgcgccgg
    12181 cgagcgacga cctcgacccg acgacggcca ggtcgaaggg atacgacctc gtcctcaacg
    12241 gctacgaggt gggtggcggg agcatccgga tccaccggcc cgaggtgcag gagcaggtct
    12301 tcgcggtgct ggggctgacc gaggccgaga tcgaagagaa gttcggccat ctgatccgcg
    12361 cgttccgcta cggcgctccg ccccacggtg ggatcgcgat gggtctcgac cgcatcgtga
    12421 tgctcatggc ggggaaggag accctccggg aggtcaccgc gttccccaag gcgcagtccg
    12481 gcgccgaccc cttgacggga gcgccggccc cggtagatgt cgcgcagctc cgcgaagtcg
    12541 gcctgcaggt gttggcgcct acgaaggagc agcgagctcg ggcaggagag gagtgaccgt
    12601 ggagaccttg cacgccgacg acgtcgagct gctcaccggg aagaacttcg cccacctcgc
    12661 ggtggttcga ccggacggca caccgcatgt gacggtcacc tggatcgatg ccgccggcgg
    12721 gacggtgctc gtgaactccg cggtgggccg ggtgaaggac cgctacctgc ggatcgaccc
    12781 ccgcgtctcg gtgaccgtgc acgacgaggc cgatccgtac cggtggctcc gggtcgatgg
    12841 ggtcgtggag ggcttcgtga ccggaccgga ggccgaggaa cacatcgata cgctgcacca
    12901 gcggtaccac gacggcgagc gatggacgtt caaggagggt caggagcgcg tgatctaccg
    12961 gatcaggccg gatcggatcc tcaggcggta cgacggctga gcttgctgcg cagccgctcc
    13021 tgagcgtatc ggtgtcttcc ggcacatcgc cctagggccg aaggcggttc cctcagccat
    13081 cccggaggtc ctatcatgct ggcctccggc tgtgacgggg ccggagactc gggctggcag
    13141 gaaaggacat tcctctctca tggatcgacc gctcgcaact cggatgcgtc cgcgcacctt
    13201 ccaggagttc gtcggacatc accatctctt gggcgagggg aagccgctca cgaagctgat
    13261 cgacggcgga caccttccct ccacgatcct gtggggccct cccgggaccg gcaagacgac
    13321 cctcgcgcac ctgctggcgg tcgcggtcgg cgccgacttc caacagctgt ccgcggtgag
    13381 ctccggcgtg tcggacgccc gccgggtgat ggaccactcc aagacgacgt tgttccggac
    13441 cgtcctgttc gtcgacgagg tacaccgatg gaacaaggcg cagcaggaga tcctcctccc
    13501 ggcgatcgag gacgggtcga tcacgctgat cggcgccacc accgagaacc cgtaccacag
    13561 cctggtgggt ccgctgctct cacggtgcct cctgctccgt ctcgagcctc tcgagccgaa
    13621 ggagatccgc gggctgctcg agctcgccgt gatcgacgcc gagcggggga tgggtgcgct
    13681 cggcgtcacg ctcaccgacg acgcgctcga ccacctcgtg gagctgtccg ccggcgatgc
    13741 ccgcgcggcg ctcaccgctc tcgaggccgc cgtcctgctg gccgacgccg gcgagacgct
    13801 gatcgatggg gcgcgggttg ccgatgcggt ccagaagcgg atcgtcgcct acgaccgtgg
    13861 cgacagccac ttcgacgtcg tctccgcgtt catcaagagc atgcgcgggt ccgacccgga
    13921 cgcggcgctg ttctggctcg ggcggatgat ccacgccggg gaggacccac ggtacatcgt
    13981 gcggcggatg atgatcttcg cgtccgagga cgtggggctc gccgatccgc tagcgcttca
    14041 ggtcgcggtg gccgccgcgc acgcgctcga gcacgtcggc atgcccgagg ctcgtttcaa
    14101 cctcgcgcac gcggccctct acctggcacg ggcgccgaaa tcggattccg tcacggaggc
    14161 gctggacaaa gcgctgtctg atgccgactc gaccgacccc gtgccgcctc atctacgcga
    14221 cgcgcactac gccggcgccg cgaagctcgg ccacggggag gggtacgagt atccccacga
    14281 attcgagggc caccacgtgg agcagcagta tcggccggag cgattcgagg acgaggtgta
    14341 ttacgagtcg acgggtcagg gccgcgacgt cgaggtcgag cccggaaccg gcgtgcccct
    14401 gatcgaccac accgtccagg acccggcacc gcaggacccg gcaccggagg agtcggcacc
    14461 gcaggagccc gcaccggagg aggacccggc accgaagagg acccggcacc cgaagaggaa
    14521 ccggcctcag taggactcag cgtctccggg cccatgggta tgctgggcga actgcccacc
    14581 ccacggaatt tcggaggtaa cggatgacgc tccttgccct caccggcggc gagaccgccc
    14641 tgatcgtgct cgccgcgttc tggggactcc tggtcctgtt cctgtgcctc gttctgctga
    14701 acacgttccg tgtgctcgag gcgacccgtc tcaccgtgga cgcgatgcgg gaggagacgg
    14761 tcccgctcct ccgagagatc aagggctccg tggagcgaac caacgggcat ctcgatcgcg
    14821 tgggcggcgt cctcgattcc gtcgggggca tcgtgggacg cgtcgagcgg ttcacgggac
    14881 tgctggagca cgccgcctcc agcccgatcg tgaagttcct gagcgtcggc gccggcgtga
    14941 agaaggcggc cgagtcgttc ggccggagaa gcggccaaag aaccgggaag aagcggtcga
    15001 agggatccgc ggcgccgggc gcgccggcgc cctgacatgc gccgcagctt ctgggtggcg
    15061 ctcggcctcg gcgcgggagc aacgggtgcc gtcgtcgcgt cgcgatgggc caggcgccag
    15121 gcgaacaagg tcaagccggc gacgcttgcg cgcgaggcac gcggcggcct gatggatctc
    15181 tccaagctcg tcgcggagtc gatcgaggag ggcaagcggg cgatggagga gcgggaacga
    15241 gagcttcgcg cagagctcga ctcgtcgaag cgcggacccg atcccgacga gccgaccgcg
    15301 gcatgaggct cggtccgagg ggtgcggaga cccaccggta gaatccgccc gtggagggcg
    15361 agcggatccg cgagctgttc ctgtcgttct tcgaggagcg agggcacaag cgcgtaccga
    15421 gttcgtccct gatcccgcct cccgagtctg gtctgctcct cgcgaacgcg gggatgaacc
    15481 agttcatccc gatcttcctc ggacaagccg agccgtccta caggcgggcc gtcaccgtcc
    15541 agaaggtgat gcgcacgaac gacatcgaga acgtcggcac ggacgctcgt cacctcacgt
    15601 tcttcgagat gctcggcaac ttctcgttcg gcgactactt caaggccgac gcgatcgcct
    15661 gggcgcacca gctcgtcacc gagggattcg ggatcgatca cgaccggctc tgggtgacgg
    15721 tgtacgagga ggacgacgag gccgtgcaag gttgggtcga cggagtgggc ctgtctccgg
    15781 accgcatcgt ccgccggggg atgacggacg cggagggtga gctcgcgaac tactggcaca
    15841 cccacgcggc cgggccgggg ggtccctgca gcgagatctt cgtcgaccgc ggcgccaagt
    15901 acgggcccga gggcgggccg gacgtcgacg aggagcgctt catggagatc tggaacctcg
    15961 tcttcatcca ggatcggatc gacgagcggc tccggttcgt cgagcccctc cccgcgaaga
    16021 acgtcgacac gggctcgtcg ctggagcgcg tggcggtggt gctccagaac gtggacagcg
    16081 tcttcgagac cgacctgttc cggccattgc tggaggtggc ggagtccctg tccggtcgca
    16141 cgcacggcca ggatccacat gacgacaagt cgctgaagat catcgccgaa cacggccggg
    16201 ccaccgcgtt cctgatcgcg gacggtgtgc agccgtcgac cgagggtcgg ggatacgtct
    16261 tgcggcggat gctccgccgg atggtgtcgc acgcccgggc gctcggcatc gagcgcgccg
    16321 tgatggatcc gatcatcgat gtggtggtgg agcggttcgg cgacgcgtac ccgtcgctcc
    16381 gggacaacga ggcgttcgtg cggcaggtgg ccgggtcgga ggaggagcgg ttcgccggca
    16441 cgctccggca agggctcgtc ctgttcgacg acgcgaaggg gagagcccgt gacgggcggc
    16501 tcaagggaga cgacgccttc aagctctcgg acacgttcgg gttcccctta cagctcacgg
    16561 aggaactcgc gacggaagcg ggcctgtcgg tcgacgtcga tcggttcgcg gcgttgctcg
    16621 aggagcagcg cgatcgggca cgacgcgccg ccaagaaggt gccgatcggc ctcgacgccg
    16681 gggcggctcc tcccaccacg ttcgtgggct acgagaccct ggagtccgac gccacgatcg
    16741 tgacgatgct cgatccggag tcccgcgagc tgcccgtggc cgaggaggga caggatgtcc
    16801 ggatcttcct cgaccgcacc cctttctacg cggaaggggg tggccaggtg ggcgatcgcg
    16861 gcctgatccg aacgtcctcg ggcgtgatca gggtcgtcga caccgtgccc gcgggcgatc
    16921 gttcgatcct gcacacgggc gtggtggggt cgggcgaggt ccgggcgagc acggaggcac
    16981 acgccgaggt cgacgccgat cgtcgcgagg ccacggcccg agcgcacacc tccacgcacg
    17041 tcgtccacgc cacgctccgg gagctgctcg gagaccatgc tcgccaggcg gggtcgctcg
    17101 tcgagccggg acgcctccgc ttcgacttcc cgcatccctc ggcggtctcg tcggagcagc
    17161 tcgacgaggc cgagctactc gcgaaccgcc gactgacggc ggacgacgtt ccgagagact
    17221 tcgagacctc gatggaggag gcggagcgcc taggtgccgt gatgctcttc ggagagaagt
    17281 acggcgacgt cgtccgcgtg gtcgagatcg gggactactc gcgcgagctc tgcggcggca
    17341 cccacgtgcc gcggacgggg cacatcgggg tgatccggtt cctccacgag ggatcgatcg
    17401 gcgccgggat gcgccgcgtc gaggcgctcg tcgggccgga cgccctccgg gagatcaacg
    17461 ccgaacgggc gctcctgcac gatctggtgg aggcgatcgg tagcaccgat ccacggagcg
    17521 ccatcgacca cgcccggaag gtgatcgagg agaacaagcg gctccggagc gcgctgggcg
    17581 cgctccggaa gggggaccgg gacgcggtca tcgcggccct cgtggacggc gtgctcgagg
    17641 tcgacggcct cggcctcgtc gtctcggagg tggcggggga ggatgcgagc ggcctccgag
    17701 agctcgccca gaggatccgc gaccggttcc agggccgtgc ggccgccgtc gtgctcggcg
    17761 gcggggtcga cgggaaggcc cagctggtgg cggcgtcgac gcccgaggcc gtcgccaagg
    17821 gtgtcacggc gcccgagctc ctccgcgacg cggcgaccgt catcggcggc ggagccggcg
    17881 gcaaggacat cctggcgatg gccggtggcc gggacggggc cgccgtcggc cgggcgctcg
    17941 gcgggatccc cgcgcgcgta cgggagctcc tcgcggccac ctcgtgaccg cacccgggcc
    18001 ggtcctcggg ctcgatccgg gcgacgcccg gatcggggtc gcgatctccg atcccgatcg
    18061 gcgggtcgcg gtgccggtcg gtaccgtgca cgtcgggcga ccgcccggcg agctgctcgc
    18121 gatcgccgag ttggtacggg agcgaggggc gtcgctgatc gtggtcgggc ttccgctctc
    18181 cctccggggc gagcacggga ctcgtgccgg gcacgcggac gcgttcgcgg acgcgctcgc
    18241 ggatgtcgtg gcggttccca tcgagctcca ggacgagcga ctgtcgaccg tggaagccga
    18301 gcgggcgctc gccgccgcgg ggatccctgg gcgggggcgc cgccgcgtgg tcgacgcctc
    18361 cgccgccacc gtgatcctcc aggcctggct ggacgcgcgg gggcacaacg gctgaagacg
    18421 gggggcccgg tatcctcgcg gtcatgtcgg cggacccgag cctccggcgc gtggggcgag
    18481 gcagccgccc gcgccggctg tccctcgtcg tcgccctcct cctgttcctc ggcgtcgtgg
    18541 gtgccggcgc gtgggcgctg aaccactacc ggacctgcca ggagcctccg cccggtccgc
    18601 ggcgagccgt cacgttcacc gtcgaggagg acacccccgc acggcaggtc atggagggtc
    18661 tccacgacga gggggtgatc gcgtgcgggg ggttcgtggg gagcctcctc cttcggggca
    18721 ccggcaaggc agaccagatc cgcgctggct ccttcgagct cacgaccggg atgacgctcg
    18781 atgcggcgat cgacgtcctg acgacgcccc cgcccaaggt gcccaccgtc gagctcgtga
    18841 tccccgaagg gttccgtctc acgcagatcg cggaacaagt ccaggacgac ctcgggatcc
    18901 cggcgaagcg gttcctgaag gaggtggaga gcggtcgcta cgtccttccg ccgtacctgc
    18961 cggccggatc ggcgacgccc gaggggttcc tcttccccaa gagctacgag ttcatcgagg
    19021 aggggctgtc cgctcgcgtg gtagcggagg agctcctcga gcagttcggg accgaagcgg
    19081 acgccctgcc gttcgaccgg accgagcagc tcggcgtgtc cccttacgag ctcgtgatca
    19141 tcgcgtcgat gatcgaggaa gaggcccggg tcgaccggga ccgaaggttg atcgccggcg
    19201 tgatctacaa ccgcctgcgc atcgggatgg cgctcgggat cgacgccacc ctcctgtacg
    19261 acgatcccac gcccgacggg gagctctcgt cggacgatct cgcccacgac accccctaca
    19321 acacccggat caacggcggg ctcccaccca cgccgatcgc cagcccagga gagaagtcgc
    19381 tccaggcggc cctcgacccg gcggagacgg acttcctcta ttacgtgctc tgcggagcgg
    19441 acggtcacca caagttcgcg ctcacgctcg gcgagcacaa taggaacgtc gatgcgtgtc
    19501 tcggctgagg gcgggatcgg cggcgccacc cacgtcgccg ggatcgtcgg gtggccggtg
    19561 gagcacagcc tgtcgcccgc gatccacaac gcggccttcg ccgcggccgg gctcgattgg
    19621 gcctacgtgc cactgcccgt gcccccggag gctctctcgg aggccctggc cggcctcgcc
    19681 ccgctcgggt tgcggggggt caacgtcacg atgccgcaca agaccgagac cgccgagttg
    19741 ctcgaggagc tgtcggagga cgcgcgtctc ctgcgggcgg tcaacacgat cctcgtgcgc
    19801 ggtgacgtcc tcgagggcca caacaccgac gcgcccgggt tcggacggtt cctccgtcgg
    19861 gacgcgggat tcgatccggc aggtgccgag gccctgatgt acggcgcggg cggcgccgcc
    19921 cgcgcttgcg cgctcgccct cgcccgagcg ggagtgggac ggctcaccgt ggcggtgcgt
    19981 gacgaggccc gagcggcggc gctcgtgtcg atcgtcgagt cggtgggcgt tccgctcgtg
    20041 gtggtgcgtc cgagagatgt cgagggctcg gtggccgacc tcgtcgtgaa cgccacaccg
    20101 gtcggcgcac ccggggagga accggcccat cccgccctgc ctcggctcgg tccgggcgtc
    20161 ctcgtggtgg atctcctcta cgacccaccg accaccgacc tgcagcgacg ggcccgggag
    20221 gcgggggcga ccgcggtcgg gggcctcggt cacctcctcg agcaggcggc gctgtccttc
    20281 gagctgtgga ccggcacgcc cgctccgctg gacgtgatgt cggcggccgc cctcgcggtc
    20341 ctcgcagacc gatagcccca cgcggttcaa gcgcgttggc cgcacggtcg atgcctccga
    20401 ggagggatga gcgactactc ggcactcgca cagcgggtct cgttgagcat cgcggggatc
    20461 cgcggctgtc tgatgctgtc tcgcgacggc tcgatcctcg gtgcccatcc cgagggtgag
    20521 cccgaagcgg atctccggac cgcgtgggtg cgcttcgcgg gggtcggtga tcccgatcgg
    20581 tcctacgtcg agttccccga ccagatctgg gcgttcgtcc gacgtggctc ctatgcagcc
    20641 ttcgcggtgg cccaggtcgg cgtccggccg ggggtcctcg tcgacacgct cgagcaagcc
    20701 ctcctgtccg ccgagcagga gcgggtggtc gaccgagaag cgatgcgcct gcccgaagca
    20761 ctttcggctc cctcgggcaa gccacgcacc agcctgcaca tggccgaccg tcagccgacg
    20821 gccgagcccg agccggcgat cggtcgagcc gaggcggccg acggcgcctc cgcggccgag
    20881 tcgccacgcg cggacgaggt cgcctcgaca cccccgccgg atgtcgctga ccctccggcg
    20941 gcggagcccg accctccagc ggcggagccc gaccctccgg ccgacgacgt cccagaggcc
    21001 caggagggca aggaagaaga gacggaggtc gatcggatcc tcctcgcgaa agagttcgcc
    21061 ggcctgcttc aggtcccgcg ggaggatgac gaagcaagca ggtagctgca tctgcacgac
    21121 catccggcgg aagacctaga gcggacggac cggcgatgac caccgtgaag cggaagtcga
    21181 agcaactcgg gcagatcttg atcgagctcg gattgatcac gtctgaacag ctcgacgcgg
    21241 ccctggcgga gcaccagaag acaccgaagg cgctcggccg ggtcctgatc gacctcggca
    21301 tgatcaagga ggccgatctc gtccgtgcgc tcgccgagca ggtgggactg gagttcgtcg
    21361 atctcgacga cacccagatc gaccccgcct ccaccgccct cttgccggaa gcgctcgctc
    21421 gccggtaccg agccctcccg atcggcgaac gcgacgggaa gctcctcgtc gcgatgtccg
    21481 accccgccaa cgtctacgcg ctcgatgaca tccgcacgat cacgggtcgc gaggtccagc
    21541 ccgtggtggc gacgtccgcc gacgtcgagc aggcgatcca gaagttcgcc ggccttgact
    21601 cccaggtcga gcagctggcc gcagaggccg cggagggcag cgagggggag gagctcgacc
    21661 tcgaggccgc cctcgaggac gcgcccatcg tcaagctcgt caacgcgatc atgacccagg
    21721 cggtcgccga gcgggcgtcg gacgtccaca tcgagccggg agagcgcgac gtgcgcatcc
    21781 gcttccgggt cgacggcgtg ctccacgagc cgatgcggcc gcagcccaag aacctccagg
    21841 gcggactgat ctcccggctc aaggtcatgg ccgatctgaa catcgcggag aagcgcgtgc
    21901 cccaggacgg gcggatctcg atgaaggtcg gcacccggca gctcgacatg cgtgtcgcga
    21961 cgcttccgac cgtgcacggc gagaaggtcg tgatccgcgt cctcgacaag agcaacgcga
    22021 tgttggacct cgccgatctc ggcttcctgg aggactccta ccatcggttc gccacggcgt
    22081 tctcgaagcc gtacggcgcg atcctcgtga ccgggccgac cgggtcggga aagtcgacca
    22141 cgatgtactc gaccctcaac atcctgaacg aggaatccaa gaacatcatc acggtggagg
    22201 acccggtcga gtaccggatg gccggcgtca atcagatcca ggtcaatccc aaggcgggac
    22261 tcacgttcgc ctctgcgctg cgatcgatcc tgcgcgccga ccccgacatc gtcctgatcg
    22321 gggagatccg ggacaaggag accgccacga tcgcgatcga agccgccctg accggtcacc
    22381 tcgtgctctc ctccttgcac acgaacgacg ccccgtcggc gatcagccgc ctcgttgaga
    22441 tggacgtgga gacgttcctg gtcgcgtcct cgatcgacgc cgtcgtcgcc cagcgtctcg
    22501 cgcggatcct gtgcgacaag tgcaagcagc cgtacgcgcc ggacgtggcc gagctccagg
    22561 cggccggcta cgcggaggac tcgtgggcgg acgtcggcga ggtcttccgg gcacccgaca
    22621 accggatcgc gaagaacgac tgcaaggcct gttccggcac gggctaccgc ggccggatcg
    22681 ggctctacga ggtgatgctc cgaagcgagg agctcgaacg actcacggtg gagcgcgcat
    22741 ctgccgatgc aatcaggacc gtcgccatcc aacaggggat gacaaccctg cgggaggacg
    22801 gtctggccaa ggtccgagca gggatcacat cgatcgagga agtcgcgagg gtggtgaagt
    22861 gagatgagct cagtggtcca gcaggatgag gacgtacaga tcccggtgcc ggagttgctc
    22921 ggtgcgctcc tggagaaggg aggttccgac ctgcacctca cggcgggttc cccgccgatg
    22981 gtgcgggtcc acggggagct ggagccggcg ggggagtacc cgtcgctcac accgagggct
    23041 ctgcagggca tggtctacgc gatcttgccg cagaagctcc gggaacggtt cgagcaggag
    23101 ctcgagctcg acatgtcgta ctccctgccg ggacgatctc gattccgtgt caacgtgttc
    23161 cagcagcgcg acgccgtcgg cgccgtgttc cgcgtgatcc cgttcgagat caagaacatg
    23221 gacgacctcg ggatccccaa cgtcgtcatg gacctcgctc gcttccagcg cggcttcgtc
    23281 gtcgtcaccg gccccacggg gtcggggaag tcgacgacgc tcgccgcgat ggtcgacatc
    23341 gtgaaccgtg agcgagccgg gcacatcatg accgtcgagg acccgatcga gttcctccat
    23401 cgccacaaga gctgcgtcgt caaccagcgc gaggtgggcg ccgacaccca cggattcgcg
    23461 caggcgctca agcacgtgct gcggcaggat cccgacgtga tcctcgtcgg tgagatgcgc
    23521 gacctcgaga cgatcgggac cgcgatcacc gcggccgaga cgggtcatct cgtcttcgcc
    23581 acgctgcaca cgcaggatgc gccccagacg atcgaccgca tcatcgacgt gttcccgccc
    23641 caccagcagc agcaggtgcg ggtccagctc tcgaccaccc tccagggcgt ggtgacgcag
    23701 cagctcgtcc cgaccacgga cgggctcggt cgtgtcgccg cggtcgaggt cctcgtgtgc
    23761 acccccgccg tccgcaacct aatccgagag ggaaagaccc accagatcta ctcgtcgatg
    23821 caggcgggcg ggcgctacgg gatgcagacg atggacatgt cgctcgccca gcacatcaag
    23881 gcgggcaggg tgtcacagca ggtcgcgttc gagcgctgcc acgatcccga agagctccag
    23941 cggctcctgg gcggctcgtt cacgagcggt ccgtccgacg gctacgacag ccccgccgct
    24001 ttcgggtcct cctacgaggg ctccggcggc atgctgatgg gaggctgaga tggcagatac
    24061 cttccagtac aaggttcgcg atcggtccgg ggccgtcacg accggctcgc tgatcgccga
    24121 tagcgaggca ctcgtcctcg cgcgcctccg cgagcagggc ttcacgccgc tcgacgtcaa
    24181 acgcaagaag aagagcatcg gccagatcga gttcggcggc aagaaggtca agctcaagga
    24241 ggtcgcgatc ttctcccgcc agttcgcgac gatggtcaac tccgggctgc cgatcctgcg
    24301 ggcgatcgcg atcctggccg agcagagcag caacaaggag ctcgcgcggg tgctgaacga
    24361 ggcccgcctg gatgtggagc aagggtcctc cctctccgga gcactggcga agcacgacca
    24421 cgtcttcaac aacctctaca tcgcgatggt ccgctcgggt gagaccggtg gctcgctcga
    24481 cacgacgctg atcgccctcg cggacatgat cgagcgagag gtacgcctcc gcgggaagat
    24541 caagtcggcg atgacctacc ccgtcgccat cgtcgctctg gtgatcttga tcatgtcggc
    24601 gatgctgttg ttcgtcgtcc cgcagttcga gtcgatcttc agccagctcg gcgggacgct
    24661 cccgctgccg acccgcggcc tgatgttcat gtcggacgcg ttcaagtcct actggtacct
    24721 cgtggcaggg agcgcggtcg gcgcccggtt cctcttccgg cgctacaaga ggacggagca
    24781 gggccgcgag gtcgtcgact cgatcaagct ccgggcgccc gtgttcggaa cgctgttcca
    24841 taagacggcg ctctcgaggt tctcgagcac gctcgcgatg ctgatgaagg ccggcgtgcc
    24901 gatcctccaa gcgctcgaga tcgtcagcga caccgtgaac aacaaggtga tcggcaaggc
    24961 gatcaccgag gtacagctgt ccgtgcgcga cggcgagtcg atcgcgaagc cgctcacgaa
    25021 gtacccggtc ttcccgccga tggtcgtcca gatgatctcg gtgggtgagg agacgggcca
    25081 ggtcgaccag atgctggaga agatcgcgca gttctacgac caagaggtcg aggcagcggt
    25141 cgatgcgctg acctccctga tcgagcccat cctgatcgcg gtcatcggtg cctgcgtcgg
    25201 gggcgcggtc gtggccctct acctcccgat gttcaacatc atcaagctga tccagtaggg
    25261 gctcgagcag cgagcgagca agcgcgagcg tagccccagg aagtcagaag agctccgtca
    25321 tcgccggacg gagatggtgg ccggcggcgc ttcggcgccg ccggccccgt catgttcggg
    25381 tcgcggagcc caccaccctt gacgcccggt cgctgcggga atccatccgg ggctcgctcc
    25441 ggttcgccat gcctcctagt cattccgggg tatttcgatg ttcctgctcc ctggggcttc
    25501 agttcgggcc gggggacgcc gaaatcagtg gggaacgcgc accgctgtgg tgcgccgact
    25561 caaaggaagg gaggcgaagg cagaatgctc aagcgtatgc gtgagcgtga ggaggggttc
    25621 accctcatcg agt
//
LOCUS       DASUYD010000158        32013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1849857, whole genome shotgun sequence.
ACCESSION   DASUYD010000158 DASUYD010000000
VERSION     DASUYD010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 32013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32013
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1849857"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF195_09020"
     CDS             complement(<1..741)
                     /locus_tag="VF195_09020"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420 hydrogenase/dehydrogenase beta
                     subunit N-terminal domain-containing protein"
                     /protein_id="HEX6580999.1"
                     /translation="MAVEKTQWRDLYDEVVLTGLCTGCTSCIVACPFHVLGYEDNVPV
                     QLQEEGPDGCSHGDKGCDICTRACPRFREWESEIDELFFGQTRKPEEIIGQYRDIYLA
                     RATGQESLMHGQDGGAVSALLVWGLENGEIDGACTSKLSDERTWDAEPTVVTDAEGVL
                     ATAGSRYTYSANPMALLKAAEMGLSKVALVGMGCQSSVTGALEFRRVNKWRRKIAWTF
                     GLLCSKSFTYDGLMVEIAQKELGLDLDHL"
     gene            860..1249
                     /locus_tag="VF195_09025"
     CDS             860..1249
                     /locus_tag="VF195_09025"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6581000.1"
                     /translation="MHSRWMRLSGAVFALTLLATACGGDEETPPDQNGGTSTESPSDA
                     GDGGGGADLTTVDFGFSPTELSVTEGQTISVTNIGETSHTFTTDDEAVDETIGAGETV
                     EIPLTGVTSGGFHCRFHPQMVGTLTVG"
     gene            complement(1280..1627)
                     /locus_tag="VF195_09030"
     CDS             complement(1280..1627)
                     /locus_tag="VF195_09030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX6581001.1"
                     /translation="MDAPVTDVISVTDKASRRIAALALKEGHALPVLRVRVTAGGCSG
                     FTYELSFADGPTEHDAVIEAADGVRVLVDPQSAPIVRGSTLEFNDSLLGGGLKMLNPQ
                     ATHECACGDSFSI"
     gene            complement(1638..3008)
                     /locus_tag="VF195_09035"
     CDS             complement(1638..3008)
                     /locus_tag="VF195_09035"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6581002.1"
                     /translation="MGSVDVFVIGGGGTGSEVAFSLARSSGLSVALAERDKLGGECNH
                     YGCVPTKVMLRSARIAKLARDGGRFGLRIPDVSVDFPAVRQRIRDVIAAQSGAGTAPF
                     ERLGVRVFLQEARLVGEHRVELADGTVIEAGKVVLATGTDAAVPPIAGIEDGPFWTNR
                     EAIWEPATPPRSLAVIGTGAVGIEFAQIYARFGAQVTALEALPHVLPQEDEEAAASIL
                     PALEADGISMRSGVRIEGARHDGTAWTLAIEGGAPVIADELLVATGRRPVFEPHDLGA
                     AGIELDDGGNPVLSETLRTRNPDVWVAGDATGDLLFTHVGSYEAEVVVKDVLGHPEAR
                     DYRVVPRVTFCDPEVASVGLTERQARAEGRDVITSVMRFSDNERANIDGSTHGLVKVV
                     ADRGTGELLGGHIVGEEAGALIHEIVVLMAARTPATAVHRAIHAYPTLSESVKGALLG
                     IAEQVG"
     gene            complement(3014..4225)
                     /locus_tag="VF195_09040"
     CDS             complement(3014..4225)
                     /locus_tag="VF195_09040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX6581003.1"
                     /translation="MVGEDVDGPSSSVATAAAAAVDPDEVLRHARALLAAPSENPGGT
                     EDLAAEVATEILEALGAEIRIVRSEEGRPSVIARFGSGARPRLAWNGHLDTVPTGDPS
                     SWSSGPFDGEVVDGRLVGRGACDMKGPIASAIGAIAAIRRAGIRLGGSLELHLVADEE
                     HAGVHGTRVLRDQGLLDQDAAIVGEPTEMEIALAERGGAWVTAVAHGKAAHGSQPHRG
                     VNAILTMSRFLLRLPEVLPELIHPLVGAPTVNVAMVAGGSAPNVVPDRCEVEIDRRIV
                     PGESDRDAVLAPFDRLVDDLVAEQRDTSIEITLREWTEAAETTGDSAIAAIVRDAIAA
                     DTGGPPPFVGFTGITDARFYINDAKIPTVIAGPGSLSVAHTADESIAVEEMVTAARSY
                     ARIFVGYLGTV"
     gene            4234..4818
                     /locus_tag="VF195_09045"
     CDS             4234..4818
                     /locus_tag="VF195_09045"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX6581004.1"
                     /translation="MLHAPSLQETLRSTLEQTPTYAPASGDRLAAVLAPLVETPEPSL
                     IFTVRSDSLSRHAGEVSFPGGLVDEGESPADAARREAFEEIGLDPTLPRLVGALPPVH
                     TYVSAILVIPFVGLLHLPPDLFPAEAEIKEVVRVPLARLATHEEPMELPRADGSIWHG
                     WSYPLEGHTVWGATGLMVHSFLEILRAEASWTLN"
     gene            4803..5270
                     /locus_tag="VF195_09050"
     CDS             4803..5270
                     /locus_tag="VF195_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015845376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX6581005.1"
                     /translation="MDAELIARRNELRSLLGEARVIAIVGISSKKWRPSHEVASYLQD
                     HGYRIIPINPNETEVLGEPAYPTLLDIPEGVRVDVVDVFRRPEQTPDVARDAVMIGAR
                     TLWLQEGIVSEEAARIAREGGLDVIMGVCIKKVRQRLMAAQDPTDERQGGTDR"
     gene            5267..5428
                     /locus_tag="VF195_09055"
     CDS             5267..5428
                     /locus_tag="VF195_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004008716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEX6581006.1"
                     /translation="MKWEYFVAPLLEHNPGEILNSFGEDGWELVTILQQQTPAGGVSM
                     VAYLKRQVA"
     gene            5436..6086
                     /locus_tag="VF195_09060"
     CDS             5436..6086
                     /locus_tag="VF195_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581007.1"
                     /translation="MDGTPFSRIDHAVLAVRDLDASAGRLREEHGLRFAPGGRHPQWG
                     TANMIAPLGDDYLELLGVVDEDVGTSTVLGRTLLELSADGDRWFSICLADDDIEATAG
                     RLGLTVQPGSRTRPDGTDVRWRGAGIEERGPDLWLPFFISWDVPAALHPGAPLAEHLV
                     AVERIAWAEVGGDEEGLREWLGGADAPIRVVDDGGDRRVRAVALAVSDGDEIVLRS"
     gene            complement(6161..7285)
                     /locus_tag="VF195_09065"
     CDS             complement(6161..7285)
                     /locus_tag="VF195_09065"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX6581008.1"
                     /translation="MRFETAAIHVGQDPDERYGAVNVPIYQTSTYAQPSVGKPKVFDY
                     ARGGNPTREAFQTALAALEGGERAFAFGSGMAAETTLLLSLRPGDHVVLPDDVYGGTY
                     RLLSRVLEPWGLSYSIVDLTDLEDLRDALRDETRVVWLETPSNPMLRIVDVAAVAQVA
                     HEAGARVVVDNTFATPALQRPLELGADAVVHSVTKYIGGHSDLIGGAVVTSDTDWIER
                     LGFLANAVGAVPGPMDCYLGLRGLKTLALRMGKHSENAGSIASWLSGDPRVRRVYFPG
                     LPDHPGHDVASRQMSAFGGIVSFDAASAEEAIAIAERTELFFLAESLGGVESLIEVPG
                     PMTHASVAGSPCEVPAQLVRLSVGLEHVDDLIADLDRALG"
     gene            complement(7312..8166)
                     /locus_tag="VF195_09070"
     CDS             complement(7312..8166)
                     /locus_tag="VF195_09070"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HEX6581009.1"
                     /translation="MLALGIDVGVGKGLDLVAVDEQRMPVHVLPKVSLEQVADSIREL
                     EPDVIAIDAPPAWAREGRSRLTERILAERNIRTFATPTRARGKGNPFYGWMEVGFRVF
                     RAAARIGFPRYTAGDPRGTAIEVFPHASATVLGGFLRPKGIGKKAWREGILVAQGVRT
                     DRLTSMDLIDAGLCALSGLLALEGKHFAPGDPKEGVIVLPVVTLPARPFAPSRPDTSA
                     APEPETSMLFRYCGCGAPGCQELTRGEFAPGHDAKRKSMLWRLTRDGRDAVDELRRRG
                     WRMPPEIG"
     gene            complement(8176..9549)
                     /locus_tag="VF195_09075"
     CDS             complement(8176..9549)
                     /locus_tag="VF195_09075"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010473711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX6581010.1"
                     /translation="MPVLDSFLDATGNTPLVRLHSVARGVKPTILAKLEMLNPGSSVK
                     DRIGLRMIEAAEREGLLKPGGTIVEPTSGNTGHGLAIAAAVRGYRCIFVMPDKMSQEK
                     ISLLRAYGAEVVICPTAVAPESPESYYRVADRLAAEIPGAFQPNQYHNPVNPQTHYDT
                     TGPEIWEQTEGRLTAFVCGVGTGGTISGAGRYLKERDPDVLVVGADPEGSIYSGDTPR
                     PYLVEGIGEDFMPSTFDPSVVDRYIRVSDRDSFLTARAITRQEGILVGGSSGTAVFAA
                     LAIGRELNDPEATIVVLLPDSGRQYMSKLYSDTWMLQHGMLERPDMVRVEEVLSAKSR
                     EIPPLITVNAHDKVRQAVEILQEHGISQAPVVREESIDVSQFMGSIRDRELLERVFRD
                     PDALQSDVAEVMAAPIPMVEWDAPVEVAFGELERTPAVVVTKAGQALGVLTRSDLLEF
                     LARQRRA"
     gene            9681..10724
                     /locus_tag="VF195_09080"
     CDS             9681..10724
                     /locus_tag="VF195_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581011.1"
                     /translation="MMQTGTTGEHVTDDETPQTAGAPTELETGPGAVVDPSGGGESLL
                     VVDDDPFIARLLEIELKAAGYDVRVASDGEQALSAAQERPPQLVLADVMMPNMDGFEL
                     TRRLRQDPRTATVSVIMLTARGLSADKLEGFAIGADDYIVKPFDTPELLARIRGVLNR
                     ASDKRSLSPMTGLAGNVAIEDEIEGLVSSEEPFSVLYVDLDRFKAYNDRYGFLRGDEA
                     IRETGRVIGEAARQIVGAETFVGHAGGDDFVVVVPPDQATAVAQQIVQAFDELAPTLY
                     DLDDQTNGYIEVTNRRGDLQRFPPLTLSIGIASTEKRMFRHYAEAITVAMEMKQYTKE
                     TGASSWSIDRRSS"
     gene            10774..11214
                     /locus_tag="VF195_09085"
     CDS             10774..11214
                     /locus_tag="VF195_09085"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581012.1"
                     /translation="MEVSDTMPGPPEVVWTLITDWEHQDDWMLEASGFEVIGDQREGV
                     GVEAKATVRIAGIKTRDTVRVSMWEPPRILVIDHLGWVKGSGEIQLQPVSEGTRMRWK
                     ESLFPPPRLGPIGRVGLRLARPLMRRVFLRDLRVLRGLVRMATT"
     gene            complement(11219..12412)
                     /locus_tag="VF195_09090"
     CDS             complement(11219..12412)
                     /locus_tag="VF195_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6581013.1"
                     /translation="MASDGTILHLRDQNDPIRVLLADDEPGLRVALTELLSHEERVEL
                     VGTAADAEEAIRIAESNLPDVALVDVRMPEGGGPHAARGITRVSPGTRVIALSAYEDR
                     TNVLEMLRAGAVGYLVKGTAADELVQGIEKVAQGGTSLSAEVMSGVVAELATQLRREE
                     IERDADLALRGDIERFVSGHGVWMVYHPIMDLETREAIGVEALARFGSIPVKPPDRWF
                     AEATALELGLQLELRAMKEALEGLSRIPDGAFLSINCSHRTAASLELAEQLDGMEKRI
                     VLEITEHEAIDDYGVLAEALEPLRARGLRVAVDDVGAGYASLRHALQLAPDMVKMDIS
                     LTHDIDRDAGRRALAAALISFAAETDMTIVAEGIETAGELHALRQLGVRYGQGFYLAR
                     PAPLE"
     gene            12547..14208
                     /gene="hutU"
                     /locus_tag="VF195_09095"
     CDS             12547..14208
                     /gene="hutU"
                     /locus_tag="VF195_09095"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX6581014.1"
                     /translation="MDGRGTREVRAPRGSELSCRSWPQEAALRMLMNNLDPEVAERPE
                     DLIVYGGTGRAARSWEAFDAIVRALRQLGDDETLLVQSGKPVGVFRTHEMAPRVLISN
                     AMLVPKWADWDTFRELEHAGLTMYGQMTAGSWIYIGTQGILQGTYETLAECAAQRFGG
                     TLAGTITLTGGLGGMGGAQPLAVTMNGGVALCIEVDPARIERRIKTRYLDRETDSVDE
                     AIGWAEEARGAREQLSIGLLGNCAEVEPELLARGFRPEIVTDQTSAHDPLAGYVPVGL
                     SLEEAATLRGRDPEGYQERSRTSMAEHVEAMVGFLDAGSVVFDYGNNLRAGAEAGGFD
                     RAYAYPGFVPAFVRPMFCEGKGPFRWAALSGDPEDIEKTDRAVAELFPDDDRLLRWLD
                     MAEERVAFQGLPARICWLGYGQRAKAGLRFNEMVASGELSAPIVIGRDHLDSGSVASP
                     YRETEDMKDGSDAIADWPILNALINTAAGAHWVSVHHGGGVGIGMSIHAGMVVVADGS
                     ENAAERLERVLTTDPGTGVIRHADAGYERALEVAAARGVQLPWLG"
     gene            14248..14601
                     /locus_tag="VF195_09100"
     CDS             14248..14601
                     /locus_tag="VF195_09100"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX6581015.1"
                     /translation="MKPARVGPIIERLRLEAGLTQGDLASRIGTTQAAISKIETGRTI
                     PGLELLDRIATALGRPIILTLGEPPEPPSVEERRRRVRRALGDFEFDPWARNPSSAER
                     DSLLADGLTRERFSH"
     gene            14582..15208
                     /locus_tag="VF195_09105"
     CDS             14582..15208
                     /locus_tag="VF195_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581016.1"
                     /translation="MSASRTDALLREAGRTDDRLRRHLLVAAALREALDGEPVVVGGT
                     AEEFQSGAPYHGTDLDLVGVVTADDRGALRSLGFRKRGRHWYHDTIHVAVEFPDDRID
                     GDVDRIERVDLGPGTVAIIGVDDLYLDRLKQATAPISSVEGSIEFHSALAVATGAFER
                     LDRRYVLRRIREVEQREGRIGERMRRLDRRIRHLAKRSMDDDSDRQRR"
     gene            15218..16168
                     /locus_tag="VF195_09110"
     CDS             15218..16168
                     /locus_tag="VF195_09110"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014559597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEX6581017.1"
                     /translation="MKVIEHLANATKPLVSVEIIPPRRGRNVERIYEAVSSLLPYEPP
                     FIDITSHAGETVWDEVPGGTFRRRVTRKAPGTFGLCAAIKYRFDIDPVPHVLCNGFTR
                     EETEDALIELNYLGIENVLTIRGDGERREPGEGRSANEGALELVGQVAAMNRGEYLEE
                     LVESTPTDFCIGVAAYPEKHAEAPNLERDIAVLVEKERAGAAYAVTQLFYDNEVYFRF
                     VKTARDQGVTIPIIPGLKILTRRSHVTSIPRTFNVSVPEALVADVAAVDDDGVPDAGI
                     RWAFEQTVQLFEQGVPWAHFYVMQDTGPLVSLLEMLKRAL"
     gene            16214..17740
                     /gene="hutH"
                     /locus_tag="VF195_09115"
     CDS             16214..17740
                     /gene="hutH"
                     /locus_tag="VF195_09115"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEX6581018.1"
                     /translation="MTVAIGEALTLADVAAVAAGADVAFPISARQRVATARTVVDDAV
                     ASDDVVYGVTTGFGALANVRVDPSKASDLQHGIVRSHATAVGRPLPRDEARAMLLLRA
                     HVLALGHSGVRPELIDLMVAMLNADVIPVVPEQGSLGASGDLAPLANLALPLIGAGQV
                     LTETGVEPATDAFRRTHLAPLRLDAKEGLALVNGTQGMLAIGAIAAERLSLLARTADV
                     VAAMTIEAALGTDAPFDFRLQRLRPHPGQAVSASNLRLLLEGSPILASHRDSEHLVQD
                     AYSLRCAPQVHGATRDVLAFARGVLEIELNAVSDNPIVLSDDGDIVSGGNFHGQPVAV
                     AMDALALATVGLSSISERRLYRLLDPALSNGLPAFLVPESGLNSGFMLVQYTAASLVS
                     ESKSLAHPASVDSIPSSAGQEDHVSMGMTAARHAREVVANAETVAALEALGAAQALDL
                     RAPLEPGPAALAVLHALRAAVPFYDADREFGPDIAAAVNLVRAGELAAAAETVTGPLG
                     "
     gene            complement(17676..18965)
                     /gene="hutI"
                     /locus_tag="VF195_09120"
     CDS             complement(17676..18965)
                     /gene="hutI"
                     /locus_tag="VF195_09120"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEX6581019.1"
                     /translation="MARGAVVAATLATARGPLPARGVAATAAVFAPGGVAWDDDGIVT
                     FVGDADDLPSEPVTLGRVDEGTIVPGFVDCHVHLPFFGWRADEFEARLAGVSYRDLHG
                     REGGIYRSARMLAEAEDDDVLAFSKALTLEMAALGTTATELKTGYGLSIEGELRQLRL
                     ARRLAEAAPQTCRVTLLACHAVPEGWSREDWVRAACEELLPAAAAEGLADAVDIYVED
                     IAFSRDDLQRVGTAASAADLPLRVHADQLGQSGAAETAAGLGARSADHLNHVSDAGIA
                     ALGGGGTAAVLLPASTFTLRAAPAPVAALRDAGAALAIATDLNPGTSPISSMPEAVAF
                     ACSLYGMTPLEALTAATANPAWVLGLDAELGTLEPGKRADLVLLDGPSFAHVPYRPGH
                     DPTTATVIGGEPVDGSASRAVRSRSPRPRRARRRARG"
     gene            complement(19010..19333)
                     /locus_tag="VF195_09125"
     CDS             complement(19010..19333)
                     /locus_tag="VF195_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581020.1"
                     /translation="MKTLDIRDLVAFSDDQPRRRTLVEGAHLWSEVICLQGAQGFGPV
                     RDDGADGLLVVLAGEVATQVGKGRARMKQWEAVDVPAGQDLTVRNASDDPSVVLLVVA
                     PPPDA"
     gene            19420..20889
                     /locus_tag="VF195_09130"
     CDS             19420..20889
                     /locus_tag="VF195_09130"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX6581021.1"
                     /translation="MTTFSFSTDAPAEVKAGVLILPVFQGPEFGPGVKETGLQQAYKD
                     AKLTGKKGESLLVTKRNGDRFAAGAVLLHGVGERRNFDAAAMRKALGRVASSVGRFGH
                     AATTFPQAVKGETGDLVQAAAEGLGLGAYRFDRYKSKNEHTPLKKVTLVGTGKADAKA
                     GRQAARRAEVLVDAVSWARDLVNTPAGDLPPAGLAREAQKMAKAEGLTCKVWTEAELK
                     KRGFGGILGVGQGSVNPPRLIELTYTGDGASTPIALTGKGIAFDSGGLSIKDAKGMET
                     MKIDMGGAASILATMKAIARLKPKINVVAAIPSAENMPGGAAQKPGDVIRHYGGTTSE
                     VLNTDAEGRLILADALALLAEKKPACIIDTATLTGACMVALGTDITGAMGNDDALVDE
                     IVKAGRSTGDWIWPLPLHEEYRRLIDSNVADIKNIGDRWGGAITAAWFLAEFVGDVPW
                     VHLDIAGPAFSEKGNDLGPKGATGVPVRALVRFVLDRAA"
     gene            20886..21605
                     /locus_tag="VF195_09135"
     CDS             20886..21605
                     /locus_tag="VF195_09135"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HEX6581022.1"
                     /translation="MSQDEPASRTGPVLGVFAHPDDAEICAGGILAKWAAAGREVHLL
                     ILTNGDRGSQDAELDRAELAATRLRETDEAARVLSLTSVRVGSVHDSELENTEEVREG
                     VVRRIREVRAETVVACDPTTVFFENRYYNHSDHRVSGFVALDASYPGSGNPHFFPEQL
                     HDLQVQDVFDIWLGWSHEPNHAEDITGFLATKVDALAKHVSQLEEGIRHFEEFLGKDA
                     VEAGERIGVEHAEEFRVLDLS"
     gene            complement(21608..22402)
                     /locus_tag="VF195_09140"
     CDS             complement(21608..22402)
                     /locus_tag="VF195_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX6581023.1"
                     /translation="MDDWRTYDSVAELYGRVHAPRFAEAAQDLVRALEVVEGDRVLDV
                     GTGTGVGVEAATNAAATAVGVDRSLAMLAVGHREHPSLPLLAAQVVDLPFRDRTFDVV
                     MGNFVLAHFVKVETALFDVRRVLRPDGRVGFTAWADGKDAFQDAWLELVENVVPRDML
                     APAYAAAAPGHERLKHPEAIVETLRQAGFRRVRAERRRYQWTYARDELVDGLGTWTVG
                     RFVREMLGESGWASLMERTRAVFADRFPDPLNDFRDVLVTVGSLPG"
     gene            22479..23273
                     /locus_tag="VF195_09145"
     CDS             22479..23273
                     /locus_tag="VF195_09145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581024.1"
                     /translation="MTGVAGVMALVLAEAVAGATVFLWVGPLWTEVKPGFFKLLGAIL
                     TVLAVLTWMCVEAAVVGRFPSGDIVRATTVVAGAAIVLWTALLFLRQPAVARVVGLLS
                     VPAWLAVLVWMGGAGRQSLGLAVFQLAAGAALLGASTDGLLLGHWYLTDRRLPRRPID
                     RMTRALLASVVAAAVAVISAGFTGVETSTAINPLLTVGALAPWIALGMVAATGLIAGF
                     VHAVLKGERASAVQSATGFYYLAVVTAFTAEVAVKTRFLDTSFGPG"
     gene            23273..23887
                     /locus_tag="VF195_09150"
     CDS             23273..23887
                     /locus_tag="VF195_09150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX6581025.1"
                     /translation="MVAAVVAGGVLAQAAMWWLISARRVPFWPATSLTFAVLGIAAVL
                     VGVPGSIDVSSLLIGAGSGAALYGATRVVVAGPLERIPSFASSVSDVYGRSLEVPGTA
                     VWVLTLAIAIPGEELFWRGLVLSELQGITSVVVGAGLAWFGYVVANAVLRNLSVLAAA
                     VVGGALWTVLGSVRDVGAAVASHLVWTSLMLLWPPRAARGKVPS"
     gene            23884..24831
                     /locus_tag="VF195_09155"
     CDS             23884..24831
                     /locus_tag="VF195_09155"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6581026.1"
                     /translation="MSFLSTAVQNVLEQGSFCAVATTGPTGPHCTPLVFAYSGGRLWF
                     TTSRRSVKTRAWKADPSAAGLVRHGELAVAFTGTAKLYDALDRNTWGDAVAGATSIAR
                     ATAAFSRKNARFFAGYAIDAKQVPFAWAPPGRVFVGIELARTALVDAEGVQEGKSRWA
                     GTSTSHGTFRRSAKGDDPLAVLPREVRQLLGAHGEGALTLTGDRGPVVVPARWRADEH
                     ALYAALPAETLALAVAGPDAPAALTADEASSWRARDMVGAMLQGTASIYLLDELGSGA
                     KTARGLATEIDPDAGALVRLAPSRAVWWRGWSSGSADVA"
     gene            24828..25268
                     /locus_tag="VF195_09160"
     CDS             24828..25268
                     /locus_tag="VF195_09160"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581027.1"
                     /translation="MSTAEFSVEIEASPERVWEVVSDPANIPHWERHVVSVQVPDEGL
                     EEDSTYSVVMAFMGVRVRVRGEILRWEPPSHATIRLRGPLDATIETSIARLPRERSLL
                     RHEVTYRFRGPLGRLVAAGLNAVGGAQLGIKHGTLGQKRQIEGG"
     gene            complement(25265..25561)
                     /locus_tag="VF195_09165"
     CDS             complement(25265..25561)
                     /locus_tag="VF195_09165"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEX6581028.1"
                     /translation="MRREPDDRNDAVPEGKPSFDYRREGLGGKATALIAAGILLLIFF
                     LQNLDAANIDFLFWDWDMAIALAIGIAAVLGFVLGWGFAWIRRRAKGDRTPSRD"
     gene            25630..26247
                     /locus_tag="VF195_09170"
     CDS             25630..26247
                     /locus_tag="VF195_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6581029.1"
                     /translation="MKAGRIVWSGLLAAGGGLIGHAALRTEDGREFDENLFAAANAEH
                     GERADRFFGGVTELGSLYAAAAAAGALALAGRRREGARALAGAGATWLLLQGLKRVVD
                     RPRPFHSNPEGTRRMIAVPHGTSWPSSHPAVLTTFTRVAARELGIGVIGRVALTGLDA
                     SVAASRVSLGVHYPSDVASGFLFGRAVARLWPGAGVGLRGRTGRG"
     gene            26257..27231
                     /locus_tag="VF195_09175"
     CDS             26257..27231
                     /locus_tag="VF195_09175"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6581030.1"
                     /translation="MPASLASVGWPVLDRFRFGDSIAISPHGLGIAVGFMVGAWLFTK
                     LAVRRGVPVEAANSVVFWSLIGAVVGARVAYVIAHVSEFESPLEWVQIWKGGISLLGG
                     IAGATIANAVNIKRQAYRFRFFQIADAIVPGLALGISVGRIGDLIIGDHLGKPTSWLL
                     AWTYEGGTLAPPFSCTPEIADTGGRCTAVLQGTFTQTIDRTQAVLRDGGEVVARGIGV
                     HQTALYDMLLAGLLFAFLWWFIGSPRREGVATLVFALSYGCVRLLEDSLRIDKRFGPF
                     TGSQWTALTVAIVAAGFLIVWAASKPPGATTPGRGDSIPDDVEPVSTS"
     gene            complement(27235..28239)
                     /locus_tag="VF195_09180"
     CDS             complement(27235..28239)
                     /locus_tag="VF195_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX6581031.1"
                     /translation="MDVLSYRPDPSEFAWTFGGVPPVRTVRPGDILELWTEDAFGGKV
                     RGPDDHVSRVIEFPFVNPQTGPFFVEGAEPGDTLAIHFISIEPSRDWAASCTVPLFGA
                     LTTTARTAMLHDPLPERVWIYEVDRNARTVAYRATEGGHTATLPLDPMHGTVGVAPAA
                     FEARSSLVPDAHGGNMDTPEMRAGVTCYLGVNVEGALFSIGDGHCRQGEGETCGVAVE
                     AAMDTVVAVEVVKGRPTPWPRLESDTHLMSTGSARPLEDAFRIAHADLVSWMGDEFGL
                     EALDAYQLLTQVSESPVANVVDPNYTFLTKVRKEYLPRVDTYDGVHARLRDTAVRYRG
                     "
     gene            complement(28323..31193)
                     /locus_tag="VF195_09185"
     CDS             complement(28323..31193)
                     /locus_tag="VF195_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX6581032.1"
                     /translation="MKRATRSGVTFVMLATMLGLVIGGSLPASGQSNGGPRRVAAASL
                     QRLRRDAVGDVTLRVDRGTRYVGFARVANGGDLFPGSRSATPQGKARGFFAEYAGLFG
                     IRDASAELRLASATTDAQGFTHVSYEQTYEGLPVFAGLVRAHLDRGNKLTSVNGTFVP
                     NVELNTDARLSPAQAAQKAIADVLARPPATEENADGAATAAPTGLRAASTKLEIYRMG
                     LVRNVPGSNQLVYEVEVTNGADVREFVFVHANAGKIVNRYSGVHDDLFRRVFESSFDP
                     DSQVWEEGDPFPGALTVDQQNIVNFSGHSYHFFSNAFGRDSYDGEGAEMQTVNNDPRI
                     NCPNANWNGITTNYCTGVTGDDTVAHEWGHAYTEFTHNLIYQWQSGALNESYSDIWGD
                     VVDLINGAGQDTPGGNRAVGTCSSQTSHKNWPTVIVNQPASLGRCAAAPAGFGPRLNP
                     TGVTANVVLSNDGKGSPTDGCTSPNNAQAIDGNIALVDRGTCTFTAKVANMQKVGATG
                     VMIVNNIIGGPTIMPGVDPSITIPSVMISRTAGASVKQALPEVNVTMRARATSGSSFR
                     WLSGEDDPAFDGAIRDMWDPTCIGDPGKVTDQEYFCAGDDNGGVHSNSGVPNHGFALL
                     VDGGTYNGRTVAGIGLTKAAHLYWRAGSVYQTPSSDFPDHADALLASCDDLVGVRLPN
                     LGTGSSPPGGARQAITSADCAAVGAMTEAIELRTDPTTQCDFRPILEGDAPPVCGGQT
                     ANVIYTEDFETGLDGWTLTNQGVFEGWPDLDWTTDASLPAGEAGSAAFGADPDIGDCG
                     AGAGDVSGVMRMESPSVTLPGGTMRLSFDHYIATEQGWDGGNVSMSVNGRAHALIPAS
                     AFLFNPYNATLQTAAAGNTNPLAGQPGFTGTDAGELVSSWGTSIIDLGATGARIRPGD
                     TVRFRFDMGMDGCTGIDGWYVDDVTVSVCGTPAATKEDAASV"
     gene            complement(31398..>32013)
                     /locus_tag="VF195_09190"
     CDS             complement(31398..>32013)
                     /locus_tag="VF195_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEX6581033.1"
                     /translation="PVGFNDMILPLEAHGVKVISMGHFVPEETPVIWRGPMLHKAIQQ
                     FLGDVWWGDLDFLLCDLPPGTGDVSISLASFIPGASMLVVTTPQDAARKVAERAGKMA
                     ERTNLRPLGVIENMSWYTCPHCGERETIFGEGGGREAAETLGIPLMAQVPLVPALRAG
                     GDEGVPIVVSDPDSPAAVALRDAAEAVVRATKSKVGKPLQLMAR"
BASE COUNT         4774 a        11028 c        11305 g         4906 t
ORIGIN      
        1 gaggtgatcg agatcgaggc cgagctcctt ctgcgcgatc tccaccatca ggccgtcgta
       61 ggtgaaggac ttggagcaga gcagtccgaa ggtccacgcg atcttgcgac gccacttgtt
      121 cacccgccgg aactccagcg cgccggtcac gctggactga cagcccatgc cgaccagggc
      181 caccttggac agacccatct cggcggcctt caagagcgcc atcgggttcg cggagtaggt
      241 gtaccgagag ccggccgtcg cgaggacccc ttcggcgtcg gtgaccacgg tcggctcggc
      301 gtcccaggtc cgctcgtccg agagcttcga ggtgcacgca ccgtcgatct cgccgttctc
      361 gaggccccac accaggagcg cggacacggc cccgccgtct tgaccgtgca tcagcgattc
      421 ctggccggtc gcacgggcca gatagatgtc ccggtactga ccgatgatct cctcgggctt
      481 ccgggtctga ccgaagaaca gctcgtcgat ctccgactcc cactcgcgga agcgcgggca
      541 ggcgcgggtg cagatgtcac agcccttgtc cccgtggctg cacccgtcgg gtccctcctc
      601 ctggagttgg accgggacgt tgtcctcgta ccccagcacg tggaacgggc acgcgacgat
      661 gcacgaggtg cagccggtgc acagtcccgt gagcaccacc tcgtcgtaga ggtcccgcca
      721 ctgggtcttc tcgacggcca cgggcgggag tctagtgggc accgtcgtcg cggaggcgaa
      781 gccggttcgc ccgctcaccc gtagtaccgt gcgggcccgg gagaccgggc atcacgtcgg
      841 agagggagag ggagggacga tgcactcacg gtggatgcgc ctatcggggg cggtcttcgc
      901 gctcacgctg ctcgcaacgg cctgcggtgg ggatgaggaa acgccacccg atcagaacgg
      961 tggcacctcg acggagagtc cctccgatgc gggtgacggt ggcggagggg cggatctcac
     1021 gaccgtggac ttcggcttct cgcccaccga gctctccgtg accgagggac agacgatcag
     1081 cgtgaccaac atcggagaga cctcgcacac gttcacgacg gacgacgaag cggtcgacga
     1141 gacgatcggt gcgggcgaga ccgtggagat cccgctcacc ggcgtgacga gcggcgggtt
     1201 ccactgccga ttccacccgc agatggtggg aacgctcacg gtcggctgac ggaagggccc
     1261 cgggcgacgt cgcatcgagt cagatcgaga aggagtcgcc gcaggcgcat tcgtgcgtgg
     1321 cctgtggatt cagcatcttc agcccgcctc ccaggagcga gtcgttgaac tccagggtgg
     1381 agccgcgcac gatcggtgcg ctctgcggat cgacgaggac gcggacgccg tccgcggcct
     1441 cgatcacggc gtcgtgctcg gtcggcccgt cggcgaacga cagctcgtag gtgaagccgg
     1501 agcagcctcc ggcggtcact cggacgcgga ggacgggcag cgcatggccc tccttgaggg
     1561 cgagcgcggc gatcctccgc gaggccttgt cggtgaccga gatgacatcg gttacgggcg
     1621 catccatcac ccccaggcta cccgacctgc tcggcgatgc cgagcagggc ccctttcact
     1681 gactcggaca gggtgggata ggcgtggatc gcgcggtgga cggccgtcgc gggcgttcgc
     1741 gcggccatca ggaccacgat ctcgtggatc agcgcgccag cctcctcgcc gacgatgtgg
     1801 ccaccgagga gctcgcccgt ccctcggtcg gcgaccacct tcacgagccc gtgcgtgctc
     1861 ccgtcgatgt tcgcgcgctc gttatcggag aaccgcataa ccgaggtgat cacgtctcgg
     1921 ccttcggcgc gagcttgccg ctcggtgaga ccgacgctcg ccacctccgg atcgcagaac
     1981 gtcactcgtg ggaccacccg gtagtcgcgc gcctcggggt gtcccaggac gtccttcacc
     2041 acgacctcgg cctcgtagga gccgacatgg gtgaagagca ggtcgccggt ggcgtccccg
     2101 gccacccaca catcggggtt gcgggtgcgg agcgtctccg acaggacggg gttccctccg
     2161 tcgtcgagct cgatgccggc ggccccgagg tcgtgcggtt cgaacaccgg gcgtcgaccc
     2221 gtcgccacga gcagctcgtc cgcgatcacg ggggcgccac cctcgatcgc gagggtccag
     2281 gccgtgccgt cgtgcctggc gccttcgatc cggacaccgg accgcatcga gataccgtcg
     2341 gcctcgaggg cgggaaggat cgacgcggcg gcctcctcgt cctcctgtgg caggacgtgc
     2401 gggagggcct ccagggcggt gacctgtgcg ccgaaccgcg cgtagatctg agcgaactcg
     2461 atccccaccg cgcccgtccc gatcacggcg agcgaccgcg gcggcgtcgc cggctcccag
     2521 atcgcctcgc ggttcgtcca gaacggcccg tcctcgatcc cggcgatggg aggtacggcc
     2581 gcgtcggtcc ctgtggcgag taccaccttg cccgcctcga tcacggtccc gtccgccagc
     2641 tccacgcgat gctcgcccac cagccgcgcc tcctggagga acacccgcac cccgagtcgc
     2701 tcgaacggcg cggtgcccgc tcccgactgc gccgcgatca cgtcgcggat ccgctgtcgg
     2761 accgccggga agtcgacgga gacgtcgggt atccggagac cgaagcgacc gccgtcgcgc
     2821 gcgagcttcg cgatcctcgc ggatcgcaac atcaccttcg tggggacgca gccgtagtgg
     2881 ttgcactcac cgccgagctt gtctcgctcg gcgagcgcga cggacagtcc tgaggagcga
     2941 gcgagcgaga acgcgacctc ggacccggtg ccgccacccc cgatcacgaa gacgtcgacg
     3001 gagcccacgc gctctagacg gtcccgaggt acccgacgaa tatccgtgcg tacgagcgcg
     3061 ccgcggtgac catctcctcg acggcgatcg actcgtccgc cgtgtgcgcg acggacagcg
     3121 accccggtcc cgcgatcacg gtggggatct tcgcgtcgtt gatgtagaag cgggcatcgg
     3181 tgatcccggt gaaaccgacg aatggcggcg gtccgccggt gtcggcggcg atcgcgtcgc
     3241 ggacgatcgc cgcgatcgcg gagtcccccg tggtctcggc cgcctctgtc cactcccgca
     3301 gggtgatctc gatgctcgtg tcccgctgct ccgcgacgag gtcgtcaacc aatcggtcga
     3361 agggggcgag gaccgcatcg cggtcgctct caccgggcac gatccgacgg tcgatctcca
     3421 cctcgcaccg atcggggacg acattggggg cgcttccccc ggcgaccatc gcgacgttga
     3481 ccgtgggggc gccgacgagg ggatggatca gctccgggag cacctcgggg agccggagca
     3541 ggaaccgcga catcgtgagg atcgcgttca cgccgcggtg cggctgggac ccgtgggcgg
     3601 ccttcccgtg cgccacggcg gtgacccagg cgccgccgcg ctcggccagt gcgatctcca
     3661 tctcggtggg ctctccgacg atcgcggcgt cctggtcgag cagaccctga tcgcggagga
     3721 cccgggtgcc gtgcaccccg gcgtgttcct cgtcggcgac caggtggagt tccaagctcc
     3781 cccccagcct gatcccggcg cggcggatcg ccgcgatcgc gccgatcgcc gacgcgatcg
     3841 gccccttcat gtcgcaggcg ccgcgcccca cgagcctccc gtcgacgacc tcgccgtcga
     3901 aggggccgct cgaccagctc gacgggtccc ccgtggggac ggtgtcgagg tggccgttcc
     3961 acgccaggcg gggtcgggca cccgacccga agcgggcgat gacgctgggg cggccctctt
     4021 cggaacgcac gatccggatc tccgctccga gggcctcgag gatctcggtg gcgacctcgg
     4081 cggccaggtc ctcggtcccg ccgggattct cggacggggc cgcgagcagg gcgcgggcgt
     4141 ggcggaggac ctcatccgga tcgacggcgg ccgccgccgc ggtggccacg gaggacgatg
     4201 gtccgtcgac gtcctcgcct accatggctg cggatgctac acgcgccctc gctacaggag
     4261 accttgcggt ccacgctgga gcagacgccg acgtacgcgc ccgcgtcagg ggaccgtctc
     4321 gccgccgtcc tcgcgccgct cgtcgaaaca cccgagccct cgctgatctt cacggtgcgt
     4381 tcggactcgc tatcgcgcca cgcgggcgag gtctcgttcc ccggcggact cgtggacgag
     4441 ggggaatcgc ccgccgatgc cgctcgacgg gaggccttcg aggagatcgg gctcgacccc
     4501 acgctgccgc gattggtcgg cgccctccct ccggtgcaca cgtacgtgtc ggcgatcctg
     4561 gtgatcccct tcgtaggatt gctgcacctg ccgccggacc tattcccggc ggaggcggag
     4621 atcaaggagg tcgtgcgcgt gccgctcgcg cgactggcga cgcacgagga gccgatggag
     4681 ctcccgcgcg cggacgggag catctggcac gggtggtcgt atcccctcga agggcacacg
     4741 gtctggggcg cgacagggct catggtgcac tccttcctcg agatcttgcg agcggaggcg
     4801 tcgtggacgc tgaactgatc gcccgacgga acgagctcag atccctcctg ggcgaagcgc
     4861 gcgtgatcgc catcgtgggg atctcctcga agaagtggcg tccctcgcac gaggtggcgt
     4921 cctacctgca ggaccacggg taccggatca tcccgatcaa cccgaacgaa acggaggtgc
     4981 tcggggagcc ggcctacccc acgctcctcg acatccctga gggcgtccgg gtcgacgtcg
     5041 tcgacgtgtt ccgccggccc gagcagacgc ctgacgttgc gcgggacgcc gtgatgatcg
     5101 gggcccgcac cctgtggttg caggagggca tcgtgagcga ggaggcggcc cggatcgccc
     5161 gggagggtgg cctcgacgtg atcatgggtg tctgcatcaa gaaagtgcgt caacggctga
     5221 tggcggccca ggatccaacg gacgaacgac agggagggac tgaccgatga agtgggagta
     5281 cttcgtggcg ccgttgctcg agcacaaccc gggcgagatc ctcaacagct tcggggagga
     5341 tggttgggag ctcgtgacga tcctgcagca gcagacgccc gcgggaggcg tgtccatggt
     5401 cgcctacctg aagcgacagg tggcctagtc agcgcgtgga cgggacaccg ttctcgcgca
     5461 tcgaccacgc cgtgctggcc gtccgcgacc tcgatgcgtc ggcggggcgc ctgcgggagg
     5521 agcacgggct gcggttcgct ccgggcgggc gacatccgca gtgggggacg gcgaacatga
     5581 tcgccccgct cggcgacgac tacctggagc tcctcggtgt cgtcgacgag gacgtgggca
     5641 cgagcacggt cctcggtcgg acgctgctcg agctctccgc cgacggcgac cggtggttct
     5701 cgatctgtct cgccgacgac gacatcgagg cgaccgccgg gaggctgggg ctcacggtgc
     5761 agccgggctc acgcacccgc ccagacggca ccgacgtgcg gtggcgcggc gccgggatcg
     5821 aggagcgagg tcccgatctc tggctgccgt tcttcatctc ctgggacgtg cccgcggcgc
     5881 tccatccggg cgcaccgctc gccgagcacc tcgtcgcggt ggagcggatc gcatgggccg
     5941 aggtcggcgg ggacgaggag gggctgcgcg agtggctcgg gggcgcggac gcgccgatcc
     6001 gcgtggtgga cgacggaggt gaccggcgcg tccgcgccgt ggcgctcgcg gtgtcggacg
     6061 gggacgagat cgtcctccgt tcctagcgag cgtcccttcc tgaggggcgt cgatctcctg
     6121 gcgagcgtcg atcagcgtcc cggatctgtt cgctcgctcg tcatccgagc gcgcggtcga
     6181 gatccgcgat caggtcgtcg acgtgctcca acccgacgga caggcgaacc agctgcgccg
     6241 gtacctcgca gggcgatccc gcgacgctcg cgtgcgtcat cgggccgggg acctcgatca
     6301 gcgactccac accgccgagc gactcggcga ggaagaacag ctcggtccgc tccgcgatcg
     6361 cgatcgcttc ctcggcggac gcggcgtcga aggacacgat ccccccgaac gccgacatct
     6421 gccggctcgc gacgtcgtgg ccgggatggt cggggagccc cgggaagtac acgcgccgca
     6481 ccctcggatc gccggagagc cacgacgcga tcgacccagc gttctccgaa tgcttcccca
     6541 tccggagcgc gagcgtcttc aacccgcgga gacccaggta gcagtccatc ggccccggga
     6601 ccgcgccgac cgcgttggcg aggaacccga gccgttcgat ccaatcggtg tcgctcgtga
     6661 cgacggcacc tccgatcaga tcggagtgac caccgatgta cttcgtcacg ctgtggacga
     6721 cggcgtcggc gccgagctcg agcgggcgct gcagcgcggg ggtcgcgaac gtgttgtcga
     6781 cgacgacccg tgcgccggcc tcgtgcgcta cctgggcgac cgccgccacg tcgacgatcc
     6841 ggagcatcgg attcgacggt gtctcgagcc agacgacgcg ggtctcgtcg cggagggcgt
     6901 cccgcaggtc ctcgagatcg gtgaggtcga cgatcgagta cgacagcccc cagggctcga
     6961 ggacccgcga cagcagccgg taggtaccac cgtagacgtc gtccggcagc acgacgtggt
     7021 cgccgggacg caacgagagc agcagcgtgg tctccgccgc catcccgctc ccgaacgcga
     7081 atgcgcgctc gccgccctcc agggcggcga gcgcggtttg gaaggcctcg cgcgtggggt
     7141 tgcccccacg cgcgtagtcg aacaccttgg gcttgccgac ggacggctgc gcgtacgtgg
     7201 acgtctgata gatcgggacg ttgaccgcgc cataccgctc atctgggtcc tggccgacgt
     7261 ggatcgccgc ggtctcgaac ctcatccgac ctccgttcat ccgatctccg ttcacccgat
     7321 ctctgggggc atcctccatc ctcgtctcct cagctcgtcg acggcgtctc gcccgtcgcg
     7381 ggtgagacgc cacagcatgg acttgcgctt ggcgtcgtga cccggcgcga actcgccccg
     7441 cgtgagctcc tgacatcccg gggcgccgca gccgcagtag cggaacagca tggacgtctc
     7501 gggctccggg gccgccgacg tgtccgggcg ggacggcgcg aagggccgag ccggcaacgt
     7561 gacgacggga aggacgatca cgccttcctt ggggtctccg ggcgcgaagt gcttgccctc
     7621 tagggccagg aggccgctca gcgcgcagag cccggcgtcg atgaggtcca tcgacgtgag
     7681 ccgatccgtc cggacgccct gcgccacgag gatgccttcc cgccaggcct tcttcccgat
     7741 gcccttgggg cggaggaacc ctcccaagac cgtggcgctc gcgtgcggga acacctcgat
     7801 cgccgtccca cgaggatccc ccgccgtgta gcgcgggaac ccgatacgcg ccgcagcacg
     7861 gaacacccgg aagccgacct ccatccagcc gtagaacggg ttccccttgc cacgtgcgcg
     7921 cgttggcgta gcgaacgtgc ggatgttgcg ctccgcgagg atccgctcag tcagacgcga
     7981 ccgtccctcc ctcgcccacg cgggtggcgc gtcgatcgcg atcacatcgg gctcgagctc
     8041 ccggatcgag tcggcgacct gttcgaggct gaccttcggc agcacgtgca ccggcatgcg
     8101 ctgctcgtcg accgcgacga gatcgagccc cttgccgacg ccgacgtcga tccccagcgc
     8161 gagcacgggc gcagactacg cccggcgctg acgagcgagg aactcgagga ggtcgctccg
     8221 cgtcaagacg ccgagggcct gcccggcttt cgtcacgacg acggccggcg tgcgctcgag
     8281 ctcgccgaac gcgacctcga caggcgcatc ccactccacc atcgggatcg gcgccgccat
     8341 cacctcggcc acatccgact gcagggcgtc ggggtcgcgg aacacccgct ccagcagctc
     8401 acggtcccgg atcgacccca tgaattgcga gacgtcgatc gactcctccc ggacgaccgg
     8461 cgcctgggag atcccgtgtt cctgcaggat ctccaccgcc tgacgcacct tgtcgtgcgc
     8521 gttcacggtg atcaggggcg ggatctcccg ggacttcgcg gagaggacct cctcgacacg
     8581 caccatgtcg ggacgttcca gcatcccgtg ctggagcatc caggtatcgg agtagagctt
     8641 cgacatgtac tgccggccgg agtccgggag cagcacgacg atcgtcgcct ccggatcgtt
     8701 cagctcgcgg ccgatcgcca acgccgcgaa caccgcggtg ccgctggagc cgcccacgag
     8761 gatgccctcc tggcgggtga tcgcgcgtgc ggtcaggaac gagtcgcgat cggagacccg
     8821 gatgtagcga tcgacgacgg acggatcgaa cgtggagggc atgaagtcct cgccgatccc
     8881 ctcgaccaga tacgggcgcg gggtgtcgcc cgagtagatc gatccttccg gatccgcccc
     8941 gaccacgagc acgtcggggt cccgctcctt caggtagcgc ccggcgccgg agatcgtccc
     9001 gcccgtcccc acgccgcaga cgaaggccgt gagccgtccc tccgtctgct cccagatctc
     9061 gggaccggtc gtgtcgtagt gggtctgggg gttcaccggg ttgtggtact ggttcggctg
     9121 gaacgcaccg gggatctccg ccgcgagccg gtcggccacg cggtagtagc tctccgggga
     9181 ctcgggcgcg acagccgtcg gacagatcac gacctccgcc ccgtacgcgc ggagcagcga
     9241 gatcttctcc tggctcatct tgtccggcat cacgaagatg caccggtagc cgcggaccgc
     9301 cgcggcgatc gcgagcccgt ggcccgtgtt gccggacgtc ggctcgacga tcgtcccgcc
     9361 cggcttgagg aggccctcgc gctcggccgc ctcgatcatc cgcagcccga tccggtcctt
     9421 cacggagctg cccgggttca gcatctccag cttggcgagg atcgtgggct tgacgcctcg
     9481 ggccacggag tgcaggcgaa ccaggggtgt gttgcccgtg gcgtcgagga acgagtcgag
     9541 caccggcatg gtgtccccct ctcgtctgcg cttctggccc cgcggaggcc cgccccatcg
     9601 tgccacgcgt gggcgtgaac gtctcacatc cggttacccg ggcggctcca cctctggcgg
     9661 tagagtgccg atgtccagag atgatgcaga ccgggacgac gggagagcac gtgacggacg
     9721 acgagacgcc gcaaacggca ggggctccga cggagctgga gacgggtccc ggagcggtcg
     9781 tcgatccgag tggtggaggc gagagcctct tggtcgtcga cgacgacccg ttcatcgcac
     9841 gcctcctcga gatcgagctg aaggccgccg gctacgacgt tcgcgtcgcg agcgacggcg
     9901 agcaggctct ctcggctgcg caggagcgtc ccccccagct cgttctggcc gacgtgatga
     9961 tgcccaacat ggacggcttc gagctgacgc ggcgtctgcg gcaggatccc cggacggcca
    10021 ccgtctccgt gatcatgctg accgctcgcg ggctctcggc cgacaagctc gagggtttcg
    10081 cgatcggagc ggacgactac atcgtgaagc ccttcgacac gccggaactc ctggcgagga
    10141 tccgcggagt cctgaaccgc gcgagcgaca agcgatccct ctcgccgatg accggcctgg
    10201 ccggcaacgt cgcgatcgag gacgagatcg aaggtctcgt gtccagtgag gagccgttct
    10261 cggtcctcta cgtggatctc gaccggttca aggcatacaa cgaccggtac gggttcctgc
    10321 gcggagacga agcgatccgg gagacggggc gggtgatcgg cgaggcggcg aggcagatcg
    10381 tcggcgccga gacgttcgtc ggccacgccg gcggtgacga cttcgtggtc gtggtgccgc
    10441 cggaccaggc caccgcggtg gcgcagcaga tcgtgcaggc gttcgacgag ctggcgccga
    10501 cgctctacga cctcgacgac cagacgaacg gctacatcga ggtcacgaac cggcggggcg
    10561 atctccagcg gttcccgccg ctcaccctgt cgatcgggat cgcctccacg gagaagcgga
    10621 tgttccgcca ctacgccgaa gcgatcaccg tcgcgatgga gatgaagcaa tacacgaagg
    10681 agacgggcgc ctcctcgtgg tcgatcgatc gccgctcgag ctgagccact gaacgccgag
    10741 gaacgagccg cgaagcgtcg gcgcccgatc gtcatcgagg tctccgacac gatgccgggc
    10801 ccgcccgagg tcgtctggac cctgatcacg gactgggagc atcaggatga ctggatgctc
    10861 gaggcctccg gcttcgaagt gatcggcgac caacgagaag gggtcggggt cgaggcgaag
    10921 gccacggtcc ggatcgccgg tatcaagacc agggacacgg tgcgcgtgag catgtgggag
    10981 ccccccagga tcctcgtgat cgatcacctc gggtgggtca aggggagcgg cgagatccag
    11041 ctccaacccg tctccgaggg aacccgcatg cgttggaagg agtcgctgtt ccctccaccg
    11101 cggctcggtc cgatcggccg ggttggtctg cgcctcgccc gccccctgat gcgccgtgtc
    11161 ttcctgcggg atctccgtgt gctccgcggg ttggttcgga tggccacgac ctagggcgtc
    11221 actccaacgg cgcgggccgg gcgaggtaga agccctggcc gtagcggacg ccgagctgcc
    11281 ggagcgcgtg cagctcacca gcggtctcga tcccttcggc cacgatcgtc atgtcggtct
    11341 cggccgcgaa cgagatcagc gcggcggcga gcgctcgacg ccccgcgtcc cggtcgatgt
    11401 cgtgcgtgag cgagatgtcc atcttcacca tatcgggggc gagctggagg gcgtgtcgca
    11461 gactcgcgta cccggccccg acgtcgtcga cggcgacccg gagcccgcgc gcccgtagcg
    11521 gctcgagcgc ctcggcgagt accccgtagt cgtcgatcgc ttcgtgctcc gtgatctcca
    11581 agacgatgcg tttctccatg ccgtccagct gttcggcgag ctcgagggag gcagcggtgc
    11641 gatgggagca gttgatggac aggaacgcgc cgtcagggat cctcgagagt ccttccagcg
    11701 cctccttcat cgcgcggagc tccagctgga gaccgagctc cagggcggtt gcctcggcga
    11761 accagcggtc cggcggcttc acggggatgg agccgaaccg cgccagcgcc tccaccccga
    11821 tcgcctcgcg ggtctcgagg tccatgatcg ggtggtacac catccagacg ccatgtccgg
    11881 acacgaaccg ttcgatgtct ccccggagcg ccaggtcggc gtcacgctcg atctcttccc
    11941 ggcggagctg cgtggcgagt tcggcgacga ccccgctcat cacttcggcg gacaggctcg
    12001 tgcctccttg ggcgaccttc tcgatcccct ggacgagctc gtcggcggca gtgcccttca
    12061 cgaggtagcc gacggcaccg gcccgcagca tctcgagcac gttcgtgcgg tcctcgtacg
    12121 cggagagggc gatcacccgg gtcccgggcg acaccctcgt gatgccgcga gcggcgtgcg
    12181 gcccaccgcc ctccggcatc cggacgtcga cgagggcgac gtcgggcagg ttcgactcgg
    12241 cgatccggat cgcctcctcg gcatccgccg ccgtgccgac cagctcgacc cgttcctcat
    12301 ggctgaggag ctccgtgagt gcgacccgga ggccgggctc gtcgtctgcg aggagcaccc
    12361 ggatcggatc gttctggtca cgaagatgga ggatggttcc gtcagaggcc atcgcagcgg
    12421 gacgctacaa gcgaggaggg ggctgctcaa gcacccgggt agcatctttg ccgtagctcg
    12481 ggtgggggtt acccggatgg gggtatcggc caagatggtg cccaggatgg ggccaggatg
    12541 acccagatgg atggtcgcgg cacgagagag gtgcgagcac ctcgtggatc cgagctcagc
    12601 tgccgctcgt ggccgcagga agccgcgctg cggatgctga tgaacaacct cgatcccgag
    12661 gtcgccgagc gccccgagga tctcatcgtc tacggcggga ccggtcgcgc ggcgcgctcc
    12721 tgggaggcgt tcgacgccat cgtgcgggcg ctccggcagc tcggcgacga cgagaccctc
    12781 ctcgtgcagt ccgggaagcc ggtcggcgtg ttccgcaccc acgagatggc gccgcgggtc
    12841 ctgatctcca acgcgatgct cgtaccgaag tgggcggatt gggacacgtt ccgcgagctc
    12901 gaacacgccg gtctcacgat gtacggccag atgacggcgg ggtcgtggat ctacatcggg
    12961 acgcagggga tcctccaggg cacgtacgag acgctggcgg agtgcgccgc gcagcggttc
    13021 ggtggcacgc tcgccggcac gatcacgctg accggcgggc tcggagggat ggggggcgca
    13081 caaccgctcg cggtcacgat gaacggtggc gtggccctct gcatcgaggt cgaccccgca
    13141 cggatcgagc ggcggatcaa gacgcgatac ctggatcgcg agacggactc tgtcgacgag
    13201 gcgatcgggt gggcggagga agcgcgaggc gcgcgcgagc agctatcgat cggactgctc
    13261 ggcaactgcg cggaggtgga gcccgagctg ctcgcgcgcg gcttccggcc cgagatcgtg
    13321 acggaccaga cgagcgcgca cgatccgctg gccggctacg tcccggtggg cctgagcctc
    13381 gaggaggcgg cgacgcttcg gggtcgcgat cccgagggtt accaggagcg ctcccggacc
    13441 tctatggcgg aacacgtcga agcgatggtc ggattcctcg acgcaggctc tgtggtcttc
    13501 gactacggga acaatctgcg agcaggcgcg gaggcgggcg ggttcgatcg ggcgtacgcc
    13561 tacccggggt tcgtgccagc gttcgtccgc ccgatgttct gcgagggcaa ggggccgttc
    13621 cgctgggccg cgctgtcggg agacccggaa gacatcgaga agaccgaccg ggcggtcgcc
    13681 gagctcttcc cggacgacga ccggttgctg cggtggctcg acatggccga ggagcgggtc
    13741 gcgttccagg gcctccctgc acggatctgc tggctgggat acgggcaacg ggccaaggcc
    13801 gggctccgct tcaacgagat ggtggcgagc ggggagctgt cggcgccgat cgtgatcggt
    13861 cgcgaccatc tcgacagcgg gtctgtcgcc tcgccgtacc gggagaccga ggacatgaag
    13921 gacggctcgg acgcgatcgc cgactggccg atcctgaacg cattgatcaa cacggccgcc
    13981 ggcgcgcact gggtgagcgt ccatcacggc gggggtgtgg ggatcgggat gtcgatccac
    14041 gcaggcatgg tggtcgtggc cgacggatcg gagaacgcgg ccgaacggct ggaacgcgtg
    14101 cttacgacgg acccgggaac tggcgtgatc cgacacgcgg acgccgggta cgagcgagcg
    14161 ctcgaggtag cggccgcccg aggcgtccag ctcccgtggc tcgggtgaca ttgcaagata
    14221 gcaatatcac ctggtacagt cctgagcatg aagcccgcac gtgtcggtcc catcatcgag
    14281 cgcctccggc tggaggcggg cctgacacag ggcgacctgg caagccggat cgggaccacg
    14341 caggcggcga tctcgaagat cgagaccggt cggaccatcc ccgggctgga actcctcgat
    14401 cgcatcgcga ccgcccttgg gcgccccatc atcctgacgc tgggtgagcc gcccgaaccg
    14461 ccgtctgtcg aggaacggcg ccgccgcgtc cgacgggcgc tcggcgactt cgagttcgac
    14521 ccgtgggcca ggaacccgtc ctcagccgag cgggatagct tgctcgccga tgggctgacg
    14581 cgtgagcgct tctcgcactg acgcgctcct ccgggaggca ggccggaccg atgatcggtt
    14641 gcggcgtcac cttctcgtgg ctgccgcgct ccgagaggcg ctcgacgggg agcccgtggt
    14701 tgtcggcggg accgctgagg agttccagtc gggggctccg tatcacggga ccgacctcga
    14761 cctcgtcggt gtcgtgaccg cggatgatcg gggcgcgctg cggtcgctcg gattccggaa
    14821 gcgagggcgc cactggtacc acgacacgat ccatgtggcg gtcgagttcc cggacgatcg
    14881 gatcgacggg gacgtcgatc gcatcgagcg ggtcgacctc gggcccggca ctgtcgcgat
    14941 catcggcgtc gacgatctgt acctcgaccg gttgaaacaa gcgaccgcgc cgatctccag
    15001 cgtcgagggg agcatcgagt tccactccgc actcgccgtc gcaacgggcg ccttcgagcg
    15061 gctcgaccgg agatacgtgc ttcggcggat ccgcgaggtt gagcagcggg agggccggat
    15121 cggtgagcgg atgcgccggc tcgatcggcg gatccggcac ctcgcgaagc gttcgatgga
    15181 tgacgactcc gatcgacagc gccgctaggc tcccctcatg aaagtcatcg agcacctcgc
    15241 gaacgcgacg aagccgctgg tgagcgtcga gatcatcccg ccgcgtcgag gccgcaacgt
    15301 cgagcgcatc tacgaggccg tatcgtcgct gctgccctac gagccgccgt tcatcgacat
    15361 cacgagccat gcgggtgaga cggtctggga cgaggtgccc ggagggacgt tccgccgacg
    15421 tgtgacgagg aaggcgccag gcacgttcgg gttgtgcgcc gcgatcaagt atcggttcga
    15481 catcgatccg gtgccgcatg tcctctgcaa cgggttcacg cgagaggaaa ccgaggacgc
    15541 gctgatcgag ctgaactacc tcgggatcga gaacgtgctc acgatccggg gcgacggtga
    15601 acgccgcgag ccaggggagg ggcgaagcgc gaacgagggt gccctggagc tcgtcggaca
    15661 ggttgcggcg atgaaccggg gcgagtacct cgaggagctc gtcgaatcca ctccgacgga
    15721 tttctgcatc ggcgtggcgg cgtatccgga gaagcacgcg gaggcgccga acctcgagcg
    15781 ggacatcgcc gtgctcgtgg agaaggagcg ggccggggcc gcgtacgccg tgacgcagct
    15841 cttctacgac aacgaggtct acttccggtt cgtgaagacc gcgcgcgacc agggcgtgac
    15901 gatcccgatc atccccgggc tgaagatcct caccaggcgg tcccacgtga cgagcatccc
    15961 tcgcacgttc aacgtctcgg tgccggaggc gctcgtcgcc gacgtggctg cggttgacga
    16021 tgacggcgtg cccgacgccg ggatccggtg ggctttcgag cagacggtgc agctcttcga
    16081 gcagggcgtg ccgtgggcgc acttctacgt gatgcaagac accggcccgc tcgtgtcgtt
    16141 gctcgagatg ctcaagcgcg ccctctagcg ctcggaccat cggcgcggac ctccggtagt
    16201 agccttcgca gccgtgacgg tcgcgatcgg ggaggcgctc acgctggcag acgtggccgc
    16261 ggtcgcggcg ggcgccgatg tcgccttccc catcagcgcg cgtcagcgag ttgccaccgc
    16321 tcggaccgtc gtcgacgacg ccgtcgcctc ggacgacgtc gtctacggcg tcaccaccgg
    16381 cttcggcgcg ctggccaacg tgcgggtcga cccctcgaag gcatccgacc tgcagcacgg
    16441 catcgttcgg tcgcacgcga cggcggtagg gcgtccgctc ccgcgagacg aagcgcgagc
    16501 gatgctcctc ctccgcgccc atgtcctggc gctcggtcat tccggtgttc gccccgaact
    16561 catcgatctg atggtggcga tgctgaacgc cgatgtgatc ccggtggtgc cggagcaagg
    16621 atccctcggt gcatccggag acctggcgcc gctggcgaac ctcgcgctgc cgttgatcgg
    16681 cgccgggcag gtattgaccg agaccggcgt cgagcccgcg acggacgcgt tccgacgaac
    16741 acacctggcg cccctgcggc tcgacgcgaa ggagggcctc gccctcgtga acggtactca
    16801 gggcatgctc gcgatcggag cgatcgcggc cgagcggctc tcgctgctcg cccggaccgc
    16861 agacgtggtc gccgcgatga cgatcgaggc ggccctcggc acggacgccc ccttcgactt
    16921 tcggctgcaa cgactccgtc cccatccggg gcaggccgtg agcgcctcga acctccggtt
    16981 gctcctagag ggttcgccga tcctggcgtc acaccgcgac agcgagcacc tcgtgcagga
    17041 cgcgtactcg ctccgctgcg cgccacaggt ccacggggcc acgcgtgatg tactcgcgtt
    17101 cgcgcggggc gtgctcgaga tagagctgaa cgccgtgtcg gacaacccga tcgtgctgtc
    17161 cgacgacggc gacatcgtgt cgggagggaa cttccacggt cagccggtcg cggtcgcgat
    17221 ggatgcgctg gcgctcgcga ccgtcgggct ctcgagcatc agcgagcgtc gcttgtatcg
    17281 attgctcgat cccgcgctct cgaacgggct gccggccttc ctcgtgccgg agagcggact
    17341 gaacagcggg ttcatgctcg tccaatacac ggcggcctcg ctcgtctctg agtcgaagtc
    17401 gctcgcgcac ccggcgtcgg tcgactcgat cccgtcgtcg gccggtcagg aggatcacgt
    17461 ctcgatgggg atgacggccg cccggcacgc gagggaggtc gttgcgaacg ccgagacggt
    17521 cgcggcgctc gaggcgctgg gggccgcgca ggcgctcgac ctacgggcgc ccctcgaacc
    17581 gggtccagcc gcgctcgccg tgctacacgc gctgcgagcg gcggtccctt tctacgatgc
    17641 ggaccgcgag ttcggtcccg acatcgcagc cgcggtcaac ctcgtgcgcg ccggcgagct
    17701 cgccgcggcc gcggagaccg tgaccggacc gctcggctag cgctcccgtc gaccggctcg
    17761 ccgccgatca ccgtggccgt ggtcggatcg tggccgggcc ggtacggcac gtgggcgaac
    17821 gacggaccgt cgagcagcac caggtcggcg cgcttgcctg gttccaacgt cccgagctcc
    17881 gcatcgagac cgagcaccca tgccgggttc gcggtggccg cggtcagggc ctcgagcggg
    17941 gtcatcccgt agagcgaaca cgcgaacgcg accgcctcgg gcatcgagga gatcggtgag
    18001 gtgcccgggt tgagatcggt ggcgatcgcg agagcggcgc cggcgtcccg gagcgcagcg
    18061 acgggcgccg gagccgcccg gagggtgaac gtggaagcgg gcaagaggac cgccgccgtg
    18121 ccaccgccac cgagggcggc gatccccgcg tcgctcacgt gattcaggtg atcggcgctc
    18181 cgcgccccga ggcccgcggc ggtctcggcc gccccgctct gacccaactg gtcggcgtgc
    18241 acgcggagcg gcagatcggc ggccgaggcc gccgtcccca cccgttgcag gtcatcgcgg
    18301 gagaacgcga tgtcctcgac gtagatgtcg accgcgtcgg cgagcccctc cgccgcggcg
    18361 gcggggagca gctcctcgca cgccgcacgc acccagtcct cgcgggacca gccctcgggc
    18421 accgcgtgac acgcgagcag cgtgacccgg cacgtctgcg gtgccgcttc ggcgagccga
    18481 cgagcgagcc ggagctgacg gagctcaccc tcgatcgaca gcccgtatcc ggtcttgagc
    18541 tcggtcgcgg tcgtgccgag cgcggccatc tcgagggtga gggccttcga gaacgcgagc
    18601 acgtcgtcgt cctcggcctc ggccagcatg cgagcgctgc ggtagatccc gccctcccgt
    18661 ccgtggagat ctcgatacga cacgccggcg agacgggctt cgaactcgtc ggcgcgccag
    18721 ccgaagaacg gcaggtgcac gtggcagtcg acgaagcccg ggacgatcgt tccctcgtcg
    18781 accctgccca gcgtcacggg ctccgacgga aggtcgtccg cgtccccgac gaacgtcacg
    18841 atcccgtcgt cgtcccacgc cacgccgccg ggcgcgaaga ccgcggcggt ggcggcgacg
    18901 cctctcgcgg ggagcggccc tcgggcggtc gcgagcgtcg ccgccaccac cgccccgcgc
    18961 gccatcgcgg aagcctagcc ccgccgtcgc tggacgggag cccgccagct catgcgtccg
    19021 ggggcggagc gaccaccagc agcacgacgg atgggtcgtc ggaggcgttg cgcacggtca
    19081 gatcctgtcc ggccggcacg tcgacggcct cccactgttt catccgagca cgccccttcc
    19141 cgacctgcgt cgccacctcg ccggcgagca ccacgaggag cccgtctgcg ccgtcgtcgc
    19201 ggaccggccc gaagccctgc gccccctgga ggcagatgac ctcggaccag aggtgagctc
    19261 cttccaccag cgttcgtcgc cgcggctggt cgtccgagaa cgccaccagg tcccggatgt
    19321 cgagggtctt cacgagcgcc agggtaccga tgtccccggt tgcgttcgct ggctacactg
    19381 ccgctcctgc tctaggccga acgaaggagg ctgcgcgttg tgaccacgtt ctcgttctcc
    19441 acggacgcac cggcggaggt gaaggcgggg gtcctgatcc tgccggtctt ccaggggccg
    19501 gagttcggtc ccggtgtcaa ggagaccggt ctccagcagg cgtacaagga cgcgaagctc
    19561 accgggaaga agggtgagtc gctcttggtc acgaagcgga acggtgatcg gttcgccgcc
    19621 ggcgccgtgc tgctccacgg cgtgggtgag cgccggaact tcgacgcggc cgcgatgcgg
    19681 aaggcgctgg gccgggtcgc ctcctcggtc ggtcggttcg ggcacgcggc cacgacgttc
    19741 cctcaagcgg tgaagggcga gaccggggac ctggtccagg ccgcagcgga ggggctcgga
    19801 ctcggcgcct accgattcga ccggtacaag tcgaagaacg agcacacccc gctcaagaag
    19861 gtcaccctgg tcgggacggg caaggccgac gcgaaggcgg gcaggcaggc cgcccgccgc
    19921 gccgaggtcc tcgtggacgc ggtgtcatgg gcgcgtgacc tcgtgaacac gccagccggc
    19981 gacctccctc ccgccgggct cgcccgcgag gcgcagaaga tggcgaaggc ggaagggctc
    20041 acctgcaagg tgtggacgga ggccgagctg aagaagcggg ggttcggagg gatcctcggt
    20101 gtggggcagg ggagcgtgaa cccgccacgg ctgatcgagc tgacgtacac gggagacggc
    20161 gcttccaccc cgatcgccct caccggcaaa gggatcgcct tcgattccgg aggtctgtcg
    20221 atcaaggacg cgaagggtat ggagaccatg aagatcgaca tgggcggcgc ggcctcgatc
    20281 ctcgccacga tgaaggcgat cgctcggctg aagccgaaga tcaacgtggt cgcggcgatc
    20341 ccgtcggcgg agaacatgcc gggcggtgcg gcgcagaagc ccggcgacgt gatccgccac
    20401 tacgggggaa cgacctcgga ggtcctgaac accgacgccg agggacggct gatcctcgcg
    20461 gacgccctcg cgctgctcgc cgagaagaag cccgcctgca tcatcgacac cgcgacgctc
    20521 accggcgcgt gcatggtcgc gctcggcacg gacatcacgg gcgcgatggg caacgacgat
    20581 gcgctcgtgg acgagatcgt gaaggcgggc cgctcgaccg gcgactggat ctggccgctc
    20641 ccgctccacg aggagtaccg ccgcctgatc gactccaacg tcgccgacat caagaacatc
    20701 ggcgatcgat ggggcggcgc gatcacggcg gcatggttcc tcgcggagtt cgtgggcgat
    20761 gttccctggg tccacctcga catcgccggg ccggccttct ccgagaaggg caacgacctg
    20821 ggtccgaagg gcgcgacggg cgtccccgtc cgcgcgctcg tccggttcgt gctcgaccga
    20881 gcggcgtgag ccaggacgag ccggcgtcgc ggacggggcc ggtcctcggc gtcttcgctc
    20941 acccggacga cgcggagatc tgcgccggcg ggatcctggc gaagtgggcc gccgccggac
    21001 gcgaggtgca cctgttgatc ctgaccaacg gcgatcgcgg ctcgcaggat gcggagctgg
    21061 atcgagccga gctggcggcg acgcggctgc gggagaccga cgaggccgcg cgggtcctca
    21121 gcctgacgag cgtccgggtg gggtcggtcc acgatagcga gctcgagaac acggaggagg
    21181 ttcgtgaagg ggtcgtccgc cggatccgcg aggtcagggc cgagacggtc gtcgcatgcg
    21241 atccgaccac cgtgttcttc gagaaccggt actacaacca ctcggaccat cgtgtctcgg
    21301 gcttcgtcgc gctcgatgcc tcctaccccg ggagcggcaa tccgcacttc ttcccggagc
    21361 agctgcacga cctgcaggtg caggacgtgt tcgatatctg gctcggttgg tcgcacgaac
    21421 cgaaccacgc ggaagacatc accggcttcc tcgccacgaa ggtggacgca ctggcgaaac
    21481 acgtcagcca gctggaagaa gggatccgcc acttcgagga gttcctgggg aaggacgccg
    21541 tcgaggcggg ggagaggatc ggcgtcgagc acgccgagga gttccgggtc ctcgacctca
    21601 gctgagctca gcccgggagg ctaccgaccg ttacgagcac gtcccggaag tcgttcagcg
    21661 gatccgggaa acgatccgcg aacaccgcac gggtgcgctc catgagcgaa gcccagccgc
    21721 tctcgccgag catctcgcgg acgaagcggc ccacggtcca tgtgccgagt ccgtccacga
    21781 gctcgtcccg cgcgtaggtc cactggtacc gcctccgctc ggctcggacg cggcggaacc
    21841 ccgcctggcg gagggtctcc acgatcgcct cgggatgctt gaggcgctcg tggccggggg
    21901 cggctgccgc ataggcgggt gccagcatgt cccgcgggac cacgttctcc acgagctcga
    21961 gccacgcgtc ctggaacgcg tccttcccgt cggcccacgc cgtgaagccc accctcccgt
    22021 ccggtcgcag cacgcggcga acgtcgaaca acgccgtttc caccttcacg aagtgcgcca
    22081 ggacgaagtt gcccatcacc acgtcgaacg ttcggtcgcg gaaggggagg tccaccacct
    22141 gtgcggcgag gagggggagc gagggatgct cgcggtgtcc caccgcgagc atcgcgagcg
    22201 atcgatcgac gccgacggcg gtggctgccg cgttcgtggc ggcctcgacg ccgacgccgg
    22261 tcccggtgcc cacgtcgagg acgcggtcgc cctcgacgac ctcgagagcg cgtacgaggt
    22321 cctgggccgc ctccgcgaat cgaggtgcgt gcacgcgacc gtagagctcc gccacgctgt
    22381 cgtacgtgcg ccagtcgtcc atcgacgtgg gagcctactc tcgagccggt ccaacgcagg
    22441 ggcgccgata gactccgcgc gatgcgaacg ggcggatcgt gaccggggtc gcgggcgtca
    22501 tggcactcgt ccttgccgag gcggtggcgg gagccacggt gttcctgtgg gtggggccgc
    22561 tctggaccga ggtcaagcca gggttcttca agctgctcgg cgcgatcctg acggtgctcg
    22621 ccgtcctcac gtggatgtgc gtcgaggcag ccgtcgtggg acggttcccc tcgggagaca
    22681 tcgtgcgggc gacgacggtc gtggcgggtg cggcgatcgt gctctggacc gcgctgctgt
    22741 tcctccggca gcccgccgtc gcgcgcgtcg tcgggctgct gagcgtcccc gcgtggctcg
    22801 ccgtgctggt gtggatgggc ggcgccggcc gccagtcgct cggcctcgcc gtgttccagc
    22861 tcgcagccgg cgcggcgctg ctgggagcat cgacggatgg cctgctcctc ggccactggt
    22921 acctgacgga tcggaggctc ccccggcgtc cgatcgaccg catgacgagg gcacttctgg
    22981 cctccgtcgt ggccgcggcc gtcgcggtga tcagcgcagg attcacgggt gtggagacct
    23041 cgacggcgat caacccgttg ctcacggtgg gtgcgctcgc accctggatc gccctgggga
    23101 tggtggcggc gacggggctg atcgcggggt tcgtgcacgc cgtgctgaag ggcgaacgtg
    23161 cctcggccgt gcagtccgcc accggcttct attacctcgc cgtcgtgacg gcgttcaccg
    23221 ccgaggtggc cgtgaagacg cgattcctcg acacgtcctt cgggccgggg tgatggtcgc
    23281 cgcggtcgtc gctggggggg tgctcgcgca ggcggcgatg tggtggttga tctccgctcg
    23341 gcgcgtgccc ttctggcccg ccacctcgtt gacgttcgcg gtcctgggga tcgccgcggt
    23401 gctcgtcggc gtcccgggat cgatcgacgt ctcgtcgctc ctcatcggag ccggctccgg
    23461 tgccgctctg tacggcgcga cccgcgtggt ggtggcgggc cccctggagc ggatcccctc
    23521 gttcgcttca tcggtgagcg acgtctacgg acggtcgctg gaagtccctg ggacggcggt
    23581 ctgggtgctg accctcgcga tcgcgatccc gggcgaggag ctcttctggc gaggcctcgt
    23641 gctctcggag ctgcagggga tcacctcggt cgtggtcggt gccggtctgg cctggttcgg
    23701 ctacgtcgtc gccaacgcgg tcctccggaa cctctcggtc ctcgcggccg ctgtcgtcgg
    23761 cggtgcgctc tggaccgtgc tcggatcggt ccgcgatgtc ggtgctgccg tggcgagcca
    23821 tctggtgtgg acgagcctca tgctcctgtg gccgccgcgg gcggcgcgtg gcaaggtgcc
    23881 ctcatgagct tcctgtccac cgcggtccag aacgtcttgg agcaaggttc gttctgcgcc
    23941 gtggcgacga cgggaccgac cggtccccac tgcacgccgc tcgtgttcgc ctactcgggc
    24001 ggccgcctct ggttcacgac ctcgcggcga tcggtgaaga cccgcgcctg gaaggcggac
    24061 ccctcggcgg ccgggctcgt ccgccacgga gagttggcgg tggcgttcac cggcacggcg
    24121 aaactctacg acgcgctcga ccggaacacg tggggcgacg ccgtggccgg tgccacgtcg
    24181 atcgctcgag cgacggcagc gttcagccgg aagaacgcgc ggttcttcgc gggctacgcg
    24241 atcgacgcca agcaggtgcc gttcgcctgg gcaccgccgg gtcgggtctt cgtgggcatc
    24301 gagctcgcac gaacggcact ggtcgatgcc gaaggggtgc aggaggggaa gagccgctgg
    24361 gcggggacat cgacgtccca cggcaccttc cgcaggtccg ccaagggcga cgacccgctc
    24421 gccgtgttgc cgcgcgaggt gcgacagctc ctcggcgcgc acggggaagg ggccctgacc
    24481 ttgaccggag accgcggccc cgtcgtggtc ccggcgcgat ggcgggccga cgagcacgcg
    24541 ctctacgccg cgctccctgc cgagacactc gcgctcgcgg tcgcgggccc cgacgcgccg
    24601 gcggcgctca cggcggacga ggcctcatcg tggcgcgccc gggacatggt gggcgcgatg
    24661 ctgcagggca cggcgtcgat ctacctcctc gacgagctcg ggagcggggc caagaccgcc
    24721 cgtgggctcg cgacggagat cgatcccgac gccggcgccc tggtgcggct ggcgccatcg
    24781 cgcgcggtgt ggtggcgcgg ctggtcgagc ggcagcgccg acgtcgcatg agcaccgcgg
    24841 agttctctgt cgagatcgag gcgtcgccgg agcgcgtctg ggaggtcgtg agcgatcccg
    24901 ccaacatccc gcactgggag cggcatgtgg tgagcgtgca ggtacccgac gagggcctcg
    24961 aagaggattc tacctactcg gtggtgatgg ccttcatggg ggtgcgggtg agggtccgcg
    25021 gagagatcct ccgttgggag cccccttccc acgccaccat ccgactgcgg ggtcccctcg
    25081 atgcgacgat cgagacctcg atcgccaggc tgccgcgcga gcggagcctc ctccgccacg
    25141 aggtcacgta ccggttccgg ggacccctcg gcaggctggt ggccgcgggc ctgaacgcgg
    25201 tcggcggggc gcagctgggg atcaagcacg ggacgctcgg ccagaagcgg cagatcgaag
    25261 gcggctaatc tcgcgagggt gtccggtccc ccttggctcg tctccggatc cacgcgaagc
    25321 cccacccgag gacgaagccg agcacggccg cgatgccgat cgccaacgcg atcgccatgt
    25381 cccaatccca gaagaggaag tcgatgttcg cagcgtcgag gttctggagg aagaagatca
    25441 gcaggaggat cccggcggcg atgagcgcgg tcgccttgcc tcccaagccc tcacggcggt
    25501 aatcgaagga gggctttcct tccggcaccg catcgttccg atcatccggc tcgcgtcgca
    25561 cgggcgtcgg tcctcctccc aaggtcgatg gagtcgtcga cgagccgatt gtcgcatgat
    25621 gggcggccga tgaaggccgg acggatcgtg tggtcggggc tgctcgccgc gggaggcggg
    25681 ctgatcgggc atgccgccct ccgcaccgag gacgggcggg agttcgacga gaacctgttc
    25741 gcggcggcga acgcggagca cggcgagcgc gccgaccggt tcttcggagg ggtcacggag
    25801 ctgggatcgc tgtacgcggc cgcggccgcg gccggcgcgc tcgcgctcgc cggccggcgc
    25861 cgcgagggcg cccgcgccct ggcgggcgcg ggcgccacct ggctcctcct ccagggtttg
    25921 aagcgggtcg tggatcggcc acgtccattc cattcgaacc cggaggggac acgccggatg
    25981 atcgcggtgc cccacggaac gtcgtggccc tccagccacc cggcggtgct caccacgttc
    26041 acgcgggtcg cggcccgcga gctcgggatc ggcgtcatcg ggcgcgtggc gctcacgggt
    26101 ctggacgcgt cggtcgccgc gtcccgcgtc tcgctcggcg tccactaccc gagcgacgtg
    26161 gcgagtggct tcctgttcgg ccgagccgtc gccaggctct ggccaggcgc cggagtgggc
    26221 ctccgcggcc gaacaggccg tggatagact tcgtcgatgc ccgcctccct cgcatccgtc
    26281 ggctggcccg tgctcgaccg tttccgcttc ggcgactcga tcgcgatctc cccgcacggg
    26341 ctcggcatcg ccgtcggctt catggtgggt gcctggctct tcacgaaact cgcggtccgt
    26401 cgcggcgtcc ccgtggaggc cgcgaactcc gtggtgttct ggtccctgat cggggccgtg
    26461 gtcggcgcgc gtgtcgccta cgtgatcgcg cacgtctccg agttcgagag tcccctcgag
    26521 tgggtccaga tctggaaagg cgggatctcg ctgctcggcg gcatcgccgg agccacgatc
    26581 gcgaacgccg tcaacatcaa acgtcaggcg tatcgcttcc ggttcttcca gatcgccgac
    26641 gcgatcgtgc cgggactcgc gctcggcatc tcggtgggtc gtatcggcga cctgatcatc
    26701 ggcgaccacc tgggcaagcc gacgtcgtgg ctgctcgcgt ggacgtacga gggtggcacc
    26761 ctcgccccgc cgttctcctg cacgccggag atcgccgaca cgggcggtcg atgtaccgcg
    26821 gtgctgcagg gcaccttcac gcagacgatc gaccgcacgc aggcggtcct ccgcgacgga
    26881 ggcgaggtcg tcgcacgcgg gatcggcgtc caccagacgg ccctctacga catgctgctc
    26941 gccggcctcc tgttcgcgtt cctgtggtgg ttcatcggct cccctcgccg ggagggcgtc
    27001 gccacgctcg tgttcgcgct gtcctacggc tgcgtccggc tgctcgagga ctcgctccgg
    27061 atcgacaagc gcttcggtcc cttcaccgga tctcaatgga cggcactcac ggtggccatc
    27121 gtcgcggcgg ggttcctgat cgtgtgggcc gcctcgaagc cacccggcgc taccacgccc
    27181 ggccgggggg actcgatccc cgacgacgtc gagcccgtct cgacgtcgtg aacctcagcc
    27241 ccggtagcgg acggcggtgt ctcgtagtcg agcgtggaca ccgtcgtagg tatccacgcg
    27301 gggcaggtat tccttccgca ccttcgtgag gaacgtgtag ttcgggtcca ccacgttggc
    27361 gacgggcgat tcgctgacct gcgtcagcag ctggtaggcg tcgagggcct cgagcccgaa
    27421 ctcgtcgccc atccaggaca cgaggtcggc gtgggcgatg cggaacgcgt cttcgagagg
    27481 acgggccgag cccgtcgaca tgaggtgggt gtcgctctcg agtcgaggcc agggcgtcgg
    27541 acggcccttc acgacctcga ccgcgacgac cgtgtccatc gcggcctcca ctgccacgcc
    27601 gcaggtctct ccttcgccct gccggcagtg tccatcgccg atcgagaaca gcgccccctc
    27661 gacgttcacc ccgaggtagc acgtgacccc tgcccgcatc tccggcgtgt ccatgttccc
    27721 gccgtgcgcg tccgggacga gcgaggaccg ggcctcgaac gccgccgggg cgacacccac
    27781 cgtgccgtgc atcggatcga ggggcagggt ggcggtgtga ccgccttcgg tcgcgcggta
    27841 tgccaccgta cgcgcgttcc ggtccacctc gtagatccag acgcgctccg ggagcgggtc
    27901 gtgcagcatc gctgtccgag cggtggtcgt gagcgccccg aacagcggca ccgtgcagga
    27961 tgcggcccag tcgcgggacg gctcgatcga gatgaagtgg atcgccaggg tgtcacccgg
    28021 ctcggctccc tccacgaaga acggtccggt ctgcggattc acgaagggga actcgatcac
    28081 gcgcgagacg tggtcgtccg gtccgcggac cttgcccccg aacgcgtcct ccgtccacag
    28141 ctcgaggatg tcgcccggcc tgaccgtccg caccggcggc acgccgccga aggtccacgc
    28201 gaactccgac gggtccggac ggtacgagag cacgtccatc gcgcgagcat acggagttcc
    28261 ggtgggccgg tgcatcgggc gccggcggcc cggagtccgg accgccggcg ccctcgctac
    28321 gatcagacgc tcgcggcgtc ctccttggtc gccgcaggcg tcccgcagac ggagacggtc
    28381 acgtcatcga cgtaccagcc gtcgatgccg gtgcagccgt ccatccccat gtcgaaccgg
    28441 aaccggacgg tgtccccggg ccggatgcgc gcacccgtcg ctcccaggtc gatgatcgag
    28501 gttccccagg agctcaccag ctctcccgcg tcggtgccgg tgaaccccgg ctgaccggcc
    28561 aaggggttgg tgttccccgc cgcggcggtc tggagcgtcg cgttgtacgg gttgaacagg
    28621 aaggcggacg ccgggatcag cgcgtgggcc cgcccgttga cgctcatcga gacgttgcca
    28681 ccgtcccatc cctgctccgt cgcgatgtag tgatcgaacg agagccgcat cgtgccaccg
    28741 ggcaacgtga ccgatgggct ctccatccgc atcacgcccg acacgtcgcc ggcccccgcg
    28801 ccgcagtccc cgatgtcggg atcggcaccg aacgccgccg acccggcctc gccggccggc
    28861 agggatgcat cggtggtcca gtccaggtcc ggccagccct cgaacacgcc ttgattcgtg
    28921 agcgtccagc cgtccagtcc cgtctcgaag tcctcggtgt agatcacgtt ggccgtctgg
    28981 ccaccgcaga ccggaggcgc gtcaccttcc aggatcggcc ggaaatcgca ctgcgtcgtc
    29041 gggtcggtgc ggagctcgat cgcctccgtc atcgcgccga ccgccgcaca gtcggccgac
    29101 gtgatcgcct gccgagcgcc gccgggcgga gagctccccg ttccgagatt gggcagtcgc
    29161 acccccacga ggtcgtcgca ggaggcgagg agcgcgtccg cgtgatcggg gaagtcgctg
    29221 gacggggtct gatagacgga tcccgcccgc cagtacaggt gggcggcctt cgtcagcccg
    29281 atgcccgcga cggtccggcc gttgtacgtc ccgccgtcga ccagcagggc gaacccgtgg
    29341 ttcgggacgc ccgagttgga atggacgccg ccgttgtcat cgccggcgca gaagtactcc
    29401 tgatccgtca ccttcccggg gtctccgata caggtcggat cccacatgtc gcggatcgcg
    29461 ccgtcgaacg cggggtcatc ctctccggag agccaccgga aggagctgcc cgaggtggcg
    29521 cgcgccctca tcgtgacgtt cacctccggg agcgcttgtt tgaccgacgc gcccgcggtc
    29581 cgggagatca tcaccgaggg gatcgtgatc gagggatcga cccccggcat gatcgtcgga
    29641 cccccgatga tgttgttcac gatcatcacg ccggtcgctc cgaccttctg catgttcgcg
    29701 accttggccg tgaacgtgca cgttccccga tcgaccagag cgatgttgcc gtcgatcgcc
    29761 tgggcgttgt tggggctggt gcaaccgtcg gtgggcgagc ccttgccgtc gttcgacagc
    29821 acgacgttcg ccgtcacgcc ggtcgggttc agtcgcgggc cgaaccccgc gggggcggcc
    29881 gcgcacctgc cgagcgatgc cggctggttg acgatcacgg tcggccagtt cttgtggctg
    29941 gtctggctcg agcaggtgcc gaccgctcgg ttgccgccgg gcgtgtcctg tccggcgccg
    30001 ttgatcaggt cgaccacgtc gccccagatg tccgagtacg actcgttcag ggcgcccgac
    30061 tgccactggt agatcaggtt gtgggtgaac tcggtgtagg cgtggcccca ctcgtgggcg
    30121 accgtgtcgt caccggtgac gcccgtgcag tagttcgtcg tgatgccgtt ccagttcgcg
    30181 ttcgggcagt tgatgcgggg gtcgttgttg accgtctgca tctcggcgcc ctcgccgtcg
    30241 taggagtctc ggccgaacgc gttggagaag aagtgatacg agtgtccgct gaagttgacg
    30301 atgttctgct ggtccacggt cagcgcgccc gggaaggggt cgccttcctc ccacacctgg
    30361 ctatcgggat cgaagcttga ctcgaagacc cgccggaaca gatcgtcgtg cacccccgaa
    30421 tagcggttca cgatcttgcc ggcgttcgcg tggacgaaga cgaactcgcg gacatcggct
    30481 ccgttcgtga cctcgacctc gtagacgagc tggttcgagc cgggcacgtt cctgacgagc
    30541 cccatccggt agatctcgag cttggtcgag gccgcccgaa ggccggtggg agcagccgtc
    30601 gcggccccgt cggcgttctc ctccgtggcc ggcggacggg ccaggacgtc ggcgatcgcc
    30661 ttctgtgccg cttgggccgg cgagagcctg gcgtccgtgt tcaactcgac gttcggcacg
    30721 aacgtgccgt tgacggacgt cagcttgttg ccgcggtcga ggtgcgcccg gacgaggccc
    30781 gcgaacacgg ggaggccctc gtaggtctgt tcgtacgaca catgcgtgaa cccctgcgca
    30841 tccgtcgtcg ccgacgccag ccgcagctcc gccgatgcgt cccggatccc gaacagcccc
    30901 gcgtactcgg cgaagaagcc acgcgccttg ccctgcgggg tcgcgctcct gctcccgggg
    30961 aagaggtcgc caccgttcgc cacgcgggcg aagccgacgt accgcgtgcc tcggtcgacg
    31021 cgcaacgtca cgtcaccgac cgcatcacgg cggagccgtt ggagcgacgc cgctgcgacg
    31081 cgcctcgggc cgccgttcga ctgaccgctc gccggcaggc tcccgccgat caccaggccg
    31141 agcatcgtgg cgagcatcac gaaggtgacg cccgaacggg ttgctcgttt catccggtct
    31201 ctcctctcgt gccaacgacc accgatccga caggcgcgtc gtggtcgttg ccaggcccag
    31261 agagacctcg ccgcccaccc cccgatgagc gaatgcgttc gcgcttcgac gccgatcgaa
    31321 acttagctgc ggttccttgc cccgtcaagc cacggttcgg gctgcgtcgc agcgctcctt
    31381 cgatcgcatc cgtgccccta gcgagccatc agctggagcg gcttgccgac cttcgacttc
    31441 gtcgcgcgga cgaccgcctc ggctgcgtcg cgcaacgcga cggcggcggg ggagtccgga
    31501 tctgagacca cgatcggcac gccctcgtcg ccacccgccc ggagcgcggg cacgagcggc
    31561 acctgcgcca tcagcgggat cccgagcgtc tcggcggcct cgcgaccacc gccctcgccg
    31621 aagatcgtct cgcgctcgcc gcagtggggg cacgtgtacc acgacatgtt ctcgatgacg
    31681 cccagcggtc ggaggttcgt gcgctcggcc atcttgccgg cgcgctcggc gaccttgcgc
    31741 gccgcatcct gcggcgtcgt caccacgagc atcgacgcac cggggatgaa cgaggcgagc
    31801 gagatcgaca cgtcgccggt gcccggcgga aggtcgcaga gcaggaagtc gaggtcgccc
    31861 caccagacgt caccgaggaa ctgctggatc gccttgtgca acatcgggcc gcgccagatc
    31921 accggcgtct cctcgggcac gaaatggccc atcgagatca ccttcacccc gtgggcctcc
    31981 agcggcagga tcatgtcgtt gaatccgacg ggt
//
LOCUS       DASUYD010000159        11283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_969649, whole genome shotgun sequence.
ACCESSION   DASUYD010000159 DASUYD010000000
VERSION     DASUYD010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969649"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(196..345)
                     /gene="rpmH"
                     /locus_tag="VF195_09195"
     CDS             complement(196..345)
                     /gene="rpmH"
                     /locus_tag="VF195_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX6581034.1"
                     /translation="MAKGKRTYQPNTRRRAKTHGFRLRMRTRAGRSILAARRRKGRSR
                     LSVSG"
     gene            649..2022
                     /gene="dnaA"
                     /locus_tag="VF195_09200"
     CDS             649..2022
                     /gene="dnaA"
                     /locus_tag="VF195_09200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX6581035.1"
                     /translation="MLDRARAELPETTFLMWFTDVRPITLRDQVLSLAVPSQFVRERL
                     QHNHLGLIEETAEEAFGHAVKIEMDVDEGIRHEEGPLVDITTAPPAAYAAIGAPATPP
                     TMGKPLGAPGLPFPNYTFDAFVPGPSSRFAHAAAMAVAEAPLSTAYNPLFIYGGTGLG
                     KTHLLVAIGHHIWRLMPDTRIKYVTSEQFVTEFIKAVRERQGDAFRHKYREVDILLVD
                     DIQFLAKREETQTEFFHTFNHLHGAGRQIVIASDRPPHELSGLEERLVSRFRWGLCVD
                     VQPPDLETRIAILQLKAERENIPAVVPEVIEFVASKFDQSVRELEGALVRVVAWSELT
                     RQPIDIELAERALQDLIPQTEHEIPPPLILDETATYFQLSTGDLVSKSRSRPLTQARH
                     IAMYLMRECTGLSLVKIGEIFGGRDHTTALHGIKKVEDEMRSRDDTFRQVQDLTRIIR
                     GRVRGLV"
     gene            2246..3346
                     /gene="dnaN"
                     /locus_tag="VF195_09205"
     CDS             2246..3346
                     /gene="dnaN"
                     /locus_tag="VF195_09205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX6581036.1"
                     /translation="MKFRCDRDDLSEALQIVQRGVSSRPGIPALTGVLLGAADDGTLT
                     LTTTDLEVSARLSIAVQVQEAGAALVPARLLTDTVKSLSEAPVDFETDQSQAHIRCAA
                     YEGALRLLPVDDFPGLQEPSGTAISVEPAAFAEAVAQVSRAASKDEARPVLTGVLLEI
                     SREGVVLVATDSYRLAVRDLVATADGEAKAIVPERALTEAGRAAVDQKAGVEASVNAS
                     QIAFRVGSLTLTSRLIEGEFPNYRQLLPETVESRLTISRQQLLEAVRRVGLLARDTTP
                     VRLEFNALGVKLSSSSPDLGQAVETVEARYEGEDLVAAFNPQYLVDGLTAATGESVRI
                     DVRDGLKPGVIRGEGDELTYLVMPVRLPAAVG"
     gene            3369..4457
                     /gene="recF"
                     /locus_tag="VF195_09210"
     CDS             3369..4457
                     /gene="recF"
                     /locus_tag="VF195_09210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HEX6581037.1"
                     /translation="MHLEWLELRDFRNHQRTAVDRVTEGLTVVVGPNGEGKTNLLEGM
                     FFLYALRSPRSSTNAPLVREGADVAYARGEFTGIEGKALVEVEIPRKGASRVKLNKSP
                     LRRKRELRRQVRSILFGPFDLPVVIGDPSKRRDLMDDAIVALWPLREGLSTAYERTLR
                     QRNRLLKEWDGHRVGPSGLDAWDEELVRTGVPVMEARRDAVAHLATGASVEFEALSGY
                     ELLVTYDPNVTGASEEAFREQLATRRPDELLRRTSLVGPHRDDLTLAVRDLGARAFGS
                     HGETWAAALCLRLGLAAAVAAETGEPPLLLVDDPFSALDPDRRDRIAQRLADRGGQVV
                     ISVADEADVPPESSAVWDVAGGTVAVRG"
     gene            4457..4798
                     /locus_tag="VF195_09215"
     CDS             4457..4798
                     /locus_tag="VF195_09215"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEX6581038.1"
                     /translation="MPSAKGFPRRKDGPSEEGVELGEVIDNLLSEQVFARGLPVARLA
                     ASWTSVVGERLASETAPAALEGGVLTVTATSGPWGAQARFLHEEIRRRADEALGGDRI
                     TAIRIVVRNRR"
     gene            4937..6868
                     /gene="gyrB"
                     /locus_tag="VF195_09220"
     CDS             4937..6868
                     /gene="gyrB"
                     /locus_tag="VF195_09220"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEX6581039.1"
                     /translation="MATAKAKRDTYDAHSIQVLEGVEHVRLRPGMYIGTTSSRGLHHL
                     VYEVVDNAVDEAMAGRASKIVVTLHEDNSVSVQDDGAGIPVKPIPKARDRRPAVEVAL
                     TTLNAGGKFGGGGYRISGGLHGVGVSVVNALSEKMTADIMRDGFAWSQGYARGRSTSK
                     LAKGRASKKTGTTIRFWPDPEIFTEDIDFKRTELTERLRELAFLNAGLEIHLIDEREK
                     PVHKDVFRFNGGLVDFVKHLATGKDAITRVIPLGEKAEDAEVELALQWTNGYSESVYS
                     FANTINTHEGGMHEEGLKKALTNVLNRYARSKGLLKEKEENLIGEDVREGLVAILSVK
                     LREPQFEGQTKTKLGNATMRSFVETTVNAQLAEWLEEHPTEARRIIHKSQQAAKARMA
                     AKQARDLTRRKSFLEGGSLPGKLADCQLSDPAACELYIVEGDSAGGSAKQARDPATQA
                     ILPIRGKILNVEKARLHKILENLEIQALITAIGTGIGDEFELDKARYHRIVLMADADV
                     DGAHIRTLLLTYFFRHMQQLIDAGYVYLAQPPLYLLKEGKAEHYFYTEREWDSFRKKN
                     GNGSKKLDPQRFKGLGEMDAHQLWDTTMDPERRTLLKVTLDDAATADRLFTVLMGEDV
                     PSRKEFIESNAKYVQNLDV"
     gene            6884..9331
                     /gene="gyrA"
                     /locus_tag="VF195_09225"
     CDS             6884..9331
                     /gene="gyrA"
                     /locus_tag="VF195_09225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX6581040.1"
                     /translation="MAQEGLFAGRVEPVQLEEEIQRSYLDYAMSVIVGRALPEVRDGL
                     KPVHRRILWSMLESGLRPDRPHRKSVAAVGEVMKKYHPHGDAPIYDALVRMAQDFSLR
                     HPLVDPHGNFGSVDGDPPASMRYTEARLAPIAMELLRDIDSDTVDFVPNFDGYEQQPV
                     VLPARFPNLLVNGSGGIAVGMATNIPPHNLGEVIDAVIHTLDHPEATPKELMKFVKGP
                     DFPTGAVIMGRDGIRDAYETGRGSIKIRAVCSVEEGSGGRARIVVTELPYQVNKARLA
                     EKIAELVRSGKVREIADLKDHSSGRAGMRLVIDLKRGANPQVVLNQLYKHTQLQESFG
                     VIMLALVDGIPRTLNLAEAIGYYVDHQVDVVTRRARFDLRKAEDRDHIVQGLLIALDH
                     LDEVIKIIRASQDAERARTKLMSTFKLSEIQANHILDMPLRRLTKLARTELEQEHKEL
                     LDRIKYLKALLKDPKKIRGVIKEEILEIKKKHADPRRTQIRADEGSFDIEDLIAEEDV
                     VITVSRAGYVKRVPIDTFRRQGRGGKGVRGATLKEEDVVTNVFTTTTHHWLLFFTTKG
                     KVYRVKVHEVPDSSRTARGLYAANLPGVAIAGDERVSAVLDMKLYEDGRYLLFATKKG
                     MVKKTPLPEYASSRTGLAAINLKPGDELIDVKLTDGKDEVFLVSRKGQAIRFKEPLVR
                     PMGRSAAGVIGMRLAEDDEVISLSLSSDGEEMISVSQQGYGKRSPLRDYPLKGRSGKG
                     VIGHQLTKKTGQLAGAFVGSKEQDMFVISSSGIVIRVPASEIRRVGRSSQGVRTMRVE
                     EGAHVVALAPVIMQMEE"
     gene            9407..10330
                     /locus_tag="VF195_09230"
     CDS             9407..10330
                     /locus_tag="VF195_09230"
                     /inference="COORDINATES: protein motif:HMM:NF023511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HEX6581041.1"
                     /translation="MNIGLGLLVEAAAFLAAVYVVAQLRPRLPRQTADLVLGSIGCVM
                     GAGALLFQRDVSLVGGVLAPLFAAVIVVGHVRGLDLMGVRLPAWVQIPALGRRGAYAI
                     DPAETRTIEEPVQQPRSATEPEPASGFDPEPLPDDEPFPREDELVPPLGPVAPEATWP
                     STIVRPPAETRLPRRGVRRTRVEIRRLGPLSVLKFSLIFYFCIFLVIYLSLAIIWGIL
                     SASGAIDSLEELLGVIFPSAGAQSPTGELSTGDAPPLEIVTGQLFTWLFIGGCVGVAI
                     WSCINVFVAIMYNLISDIVGGIEVTLADRRD"
     gene            10433..10507
                     /locus_tag="VF195_09235"
     tRNA            10433..10507
                     /locus_tag="VF195_09235"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10468..10470,aa:Ile,seq:gat)
     gene            10560..10733
                     /locus_tag="VF195_09240"
     CDS             10560..10733
                     /locus_tag="VF195_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581042.1"
                     /translation="MGDDTEGTEKKGGFFRKLLKLAFIGAIVAAVMQFMKRRRGEDVD
                     DVEWQELPPPAGG"
     gene            10815..>11283
                     /locus_tag="VF195_09245"
     CDS             10815..>11283
                     /locus_tag="VF195_09245"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6581043.1"
                     /translation="MLEPGTPWDLAHTAARAANVKLRPLTSLAQFDQTLEVMVATWGA
                     FQLPPREMMTALAHSGNVPFGAYDGDRLVGFVLGWAGVDHGGLHVHSHMLAALPDRRH
                     RGVGYALKLGQRAQALEQDIHVARWTFDPLVARNAWLNLGKLGAVADGFGRAFY"
BASE COUNT         1940 a         3766 c         3851 g         1726 t
ORIGIN      
        1 gcggcgcgac ctcgcggatc gctgcccgaa ggacccgccg ggcccggttc cgctgcaccg
       61 cgccgccgat cttcttgccg gcgatcagcg cgaactcccc cgagccggga gcgacaaaca
      121 gtaccacccg cttcccatgc accggctccg cgtcttggaa gacgcgctgg atcgcccggg
      181 tgcgcaccgt tgcggtcagc cggagacgct gaggcggctg cggcccttgc gtcggcgagc
      241 ggcgaggatc gaccggcctg cacgggtccg catccgcaga cggaagccgt gcgtcttcgc
      301 acggcggcgc gtgttcggct ggtacgtacg ctttcccttg gccatcctcg gattccttcg
      361 cggaaggaag gccggcaacg ccgccggccg aagggaaagt atacggaaac atccctgggt
      421 ggaggggttg acaacagaaa gtgcgtcaag acggttccgg ccactcgctt ggggaaaaac
      481 cccggtgcta tagtccgccc cgcacttccc cggggtgatg gggcccccgg cgtccccgac
      541 cccgtgacct ccccggattt gggttatcca ccggtgtgga aaggctctgt ggaatctcag
      601 atggccgtcg ctttgcccga tcaagaggcg agaagagcct ggtcggccgt gctcgatcgc
      661 gctcgggccg agctgccgga gaccacgttc ctgatgtggt tcaccgatgt ccggccgatc
      721 acgctccgtg atcaggtgct ctccctcgcg gtcccgagtc agttcgtccg ggaacgcctc
      781 cagcacaatc accttgggct catcgaagag accgccgagg aggcgttcgg tcacgccgtc
      841 aagatcgaga tggacgtgga cgaaggcatc cgtcacgagg aaggcccgtt ggtggacatc
      901 accacggcgc ccccggccgc ctacgccgcg atcggcgcac cggcgacgcc gccgacgatg
      961 ggcaagcccc tcggcgctcc cggcctgccg ttccccaact acacgttcga cgcgttcgtc
     1021 cccggcccgt cgagccggtt cgcgcacgcc gccgccatgg cggtggcgga ggcaccgctg
     1081 tccaccgcgt acaacccctt gttcatctac ggaggcacgg gcctcggcaa gacgcacctc
     1141 ctggtggcga tcgggcatca catctggcga ttgatgcccg acacgcgtat caagtacgtg
     1201 acctccgagc aattcgtcac ggagttcatc aaggccgtcc gcgaacgaca gggtgacgcg
     1261 ttccggcaca agtaccgaga ggtcgacatc ctgctcgtcg acgacatcca gttcctcgcc
     1321 aagcgcgagg agacgcagac cgagttcttc cacacgttca accatctcca cggcgccggg
     1381 cggcagatcg tgatcgcgtc ggaccggccg ccgcacgaac tctccggcct cgaggaacgg
     1441 ctggtgagtc ggttccggtg gggtctgtgc gtggacgtgc agcccccgga cctcgagacg
     1501 cgcatcgcga tcctccagct gaaggccgag cgggagaaca tccccgccgt cgtgccagag
     1561 gtgatcgagt tcgtcgcttc gaagttcgac cagtccgtcc gtgagctcga aggcgccctg
     1621 gtgcgcgtgg tcgcgtggtc cgagctcacc cgccagccga tcgatatcga gctcgcggag
     1681 cgagccctgc aggacctgat cccgcagacc gagcacgaga tcccaccacc gctcatcctc
     1741 gacgagaccg ccacctactt ccaactctcg acgggggacc tggtgtcgaa gtcgcgctcg
     1801 cgaccgctca cgcaggcgag acacatcgcc atgtacctga tgcgggaatg cacgggcctt
     1861 tccctcgtca agatcggcga gatcttcggt gggcgagacc acaccaccgc cctccacggc
     1921 atcaaaaagg tcgaggacga gatgcggtct cgcgacgaca cgttccgtca ggtccaggac
     1981 ctcacgcgga tcatccgcgg ccgtgtccgg gggttggtgt gatatcggtg gacaacttcg
     2041 cgccgatggc cgttctcgat cacggaacga cgggtcccgg aagagcgcca aggacggtcc
     2101 accggccgtc cccatgtcgg acacccacgc tacgctgcgc gaacacccca cttctccact
     2161 tctccaccgc ccctactacc gctcttcatg gataagaacc aagaagcaac agtcagggaa
     2221 cagccacgac cgggaggttg atgagatgaa gttccgatgc gaccgtgatg acctgtcgga
     2281 ggcgttgcag atcgtccagc gcggcgtgtc ctcgcgccca gggatcccgg cactcaccgg
     2341 cgtgttgctc ggggctgcgg acgacggcac cctgacgctc accacgacgg acctggaggt
     2401 ctcggcacgg ctctcgatcg ccgttcaggt ccaggaggcc ggcgccgcat tggtacctgc
     2461 tcgtctgctg accgacaccg tgaagtcact ctcggaggca ccggtcgact tcgagacgga
     2521 ccagtcccag gcgcacatcc gctgcgcggc gtacgagggg gccctccggc tgctgccggt
     2581 cgacgacttc ccaggactgc aggagccgtc ggggaccgcg atctcggtcg agcctgccgc
     2641 gttcgccgag gccgtcgccc aggtatcccg ggcggcctcg aaagacgaag ccaggccggt
     2701 gctcacggga gtgctgctcg agatcagccg ggagggcgtg gtcctcgtcg caacggactc
     2761 gtaccgactc gcggtccgag atctcgtcgc caccgcagac ggcgaggcga aagcgatcgt
     2821 tcccgagcgc gcgttgacgg aggccggccg tgcggccgtc gaccagaagg ccggggtgga
     2881 ggcatcggtc aacgcttcgc agatcgcgtt ccgcgtcggc tccctcaccc tgacgtctcg
     2941 cctgatcgag ggggagttcc ccaactaccg ccagctgctg ccggagaccg tcgagagccg
     3001 actgacgatc tcgcgccaac agctcctcga agccgtccgt cgagttgggc tgctcgctcg
     3061 tgacacgacc cccgtgcgat tggagttcaa cgcgctcggc gtgaagctgt cgtcgtcgag
     3121 cccggacctc ggtcaggccg tcgagacggt cgaggcccga tacgaggggg aggacctcgt
     3181 cgcggcgttc aacccgcagt acctcgtcga cggcctgacc gcagccaccg gcgagagcgt
     3241 gcggatcgac gtgcgcgacg ggctcaagcc cggcgtgatc cgcggcgagg gtgacgagct
     3301 gacctacctc gtgatgccgg tccggctccc cgccgcggtc ggctgaaccg cggcgggcgc
     3361 ggacccggat gcacctggag tggctcgagc tccgggattt ccggaaccac caacgcacgg
     3421 ccgtcgatcg cgtcacggaa ggtctgacgg tggtcgtggg tccgaacggc gaggggaaga
     3481 ccaacctgct ggagggcatg ttcttcctgt acgcgctccg ctcaccccgc tcctccacga
     3541 acgcgccgct cgtccgcgag ggggcggacg tggcgtacgc gcggggtgag ttcacgggca
     3601 tcgagggcaa ggccctggtc gaggtggaga tcccgcggaa gggtgcgagt cgcgtgaagt
     3661 tgaacaagag cccgctccga cgcaagcgcg agctgcgccg tcaggtgcgc tcgatcctct
     3721 tcggcccctt cgacctgccc gtggtgatcg gagacccctc gaagcgacgg gacctcatgg
     3781 acgacgcgat cgtggcgctg tggccgttga gggaagggtt gtccaccgcg tacgaacgaa
     3841 cgctccgtca gcgaaaccgg ctgctcaagg aatgggacgg gcatcgcgtc ggcccgtccg
     3901 ggctcgacgc ctgggacgag gagcttgtcc ggacgggcgt gccggtgatg gaggcacgcc
     3961 gggatgccgt cgcacacctc gccaccgggg cctcggtcga gttcgaggcg ctctcgggct
     4021 acgagctcct cgtgacgtac gacccgaacg tgacgggcgc ctcggaggag gcgttccggg
     4081 agcaactcgc cacgcggcgg cccgacgagc tgctccgcag aacgtccctg gtcgggcccc
     4141 atcgcgacga cctcaccctc gccgtgcgcg acctcggagc tcgtgcgttc ggttcgcacg
     4201 gtgagacgtg ggccgccgcc ctctgtctgc gcctcggcct cgcggccgcc gtcgccgccg
     4261 agacgggcga gccgccgctc ctgctcgtcg acgatccctt cagcgctctc gaccccgatc
     4321 gccgcgatcg gatcgcgcaa cggctggcgg atcgaggggg ccaggtcgtg atcagcgtgg
     4381 cggacgaggc cgacgtgccg ccagagtcgt ccgcggtgtg ggacgtggca ggcggaaccg
     4441 tggcggtacg gggctgatgc cgagcgcgaa ggggttcccg cgacggaagg acggaccgag
     4501 cgaggagggc gtcgagctcg gcgaggtgat cgacaacctc ctgtccgagc aggtcttcgc
     4561 ccggggcttg ccggtggcgc ggctcgccgc ttcgtggacg tcggtcgtcg gcgagcggct
     4621 ggcgtcggag accgcgccgg cggcactcga aggaggtgtc ctgaccgtca cagcgacctc
     4681 cgggccgtgg ggcgctcagg cccgattcct ccacgaggag atccgccgtc gggcggacga
     4741 ggcgctcggc ggcgatcgga tcacggcgat ccgcatcgtg gtgcgaaacc gcaggtagac
     4801 agccgatttc cgcgaccgga atcgccgttc ccgtcacccc tgattgatag aatccaaggg
     4861 tacgtgcgac ggccccgcgc acggccccag ctccgttgcg tggggcggcc tgcgcggggc
     4921 accgacagga ggacgattgg ccaccgcgaa ggcgaagaga gatacctacg acgcacatag
     4981 catccaggtc ctcgagggcg tcgagcacgt ccggctccgc ccggggatgt acatcggcac
     5041 caccagctca cgcggcctgc atcacctcgt ctatgaggtc gtcgacaacg ccgtcgacga
     5101 ggcgatggcg ggccgcgcgt cgaagatcgt cgtcacgctc cacgaggaca acagcgtgtc
     5161 ggtgcaggac gacggcgccg ggatcccggt caagccgatc ccgaaggcga gggatcgccg
     5221 ccccgccgtc gaggtggctc tcaccacgct gaacgccggc ggcaagttcg gcgggggtgg
     5281 gtaccggatc tcgggcggac tgcacggcgt cggggtctcc gtcgtgaacg cgctctccga
     5341 gaagatgacc gccgacatca tgcgcgacgg gttcgcctgg tcgcaggggt acgcccgcgg
     5401 ccgatccacc tcgaagctcg ccaagggcag ggcgagcaag aagaccggga ccacgatccg
     5461 cttctggccc gatcccgaga tcttcactga ggacatcgac ttcaagagga cggagctcac
     5521 cgagcgcctc cgcgagctcg cgttcctgaa cgccggtctt gagatccacc tgatcgacga
     5581 gcgcgagaag ccggtgcaca aggacgtgtt ccggttcaac ggcggcctgg tcgacttcgt
     5641 gaagcatctc gccaccggga aggacgcgat cacgagggtc atccccctcg gtgaaaaggc
     5701 cgaggacgcc gaggtcgagc tcgcgctgca atggacgaac ggctactcgg agtccgtcta
     5761 ctccttcgcg aacacgatca acacccacga gggcgggatg cacgaggagg gcttgaagaa
     5821 ggcactcacc aacgtcctga atcggtacgc ccgctccaag gggctcctca aggagaagga
     5881 ggagaacctg atcggcgagg acgtgcggga ggggctcgtc gcgatcctgt cggtgaagct
     5941 gagggagccg cagttcgagg gacagaccaa gaccaagctc ggcaacgcca cgatgcgatc
     6001 gttcgtcgag accacggtga acgcccagct cgcggagtgg ctcgaggagc accccaccga
     6061 ggcgcggagg atcatccaca agagccagca ggccgccaag gcgcggatgg cggccaagca
     6121 ggcgcgcgac ctcacgcgcc gcaagtcctt cctcgagggc ggctcgctcc cgggcaagct
     6181 cgcagactgc cagttgagtg atccggcggc ctgcgagctc tacatcgtgg agggagactc
     6241 ggcggggggg agcgccaagc aggcgcgtga cccggccacg caggcgatct tgccgatccg
     6301 cgggaagatc ctcaacgtcg agaaggcccg gctccacaag atcctcgaga acctcgagat
     6361 ccaggccctg atcaccgcga tcgggacggg gatcggcgac gagttcgagc tcgacaaggc
     6421 gcgctaccac cgcatcgtgc tgatggcgga cgccgacgtc gacggcgcgc acatccgcac
     6481 gctgctgctc acgtacttct tccggcacat gcagcagctg atcgacgcgg gctacgtcta
     6541 cctcgcacaa ccgccgctgt acctcctgaa ggagggcaag gccgagcact acttctacac
     6601 ggagcgagag tgggactcgt tccggaagaa gaacggcaac gggagcaaga agctcgaccc
     6661 gcagcggttc aaggggctcg gcgagatgga tgcgcaccag ctctgggaca ccacgatgga
     6721 ccccgagcgt cggacgctgc tcaaggtgac gctcgacgac gcggcgacgg ccgaccggct
     6781 cttcaccgtg ctgatgggcg aggacgtccc gtcgcgcaag gagttcatcg agagcaacgc
     6841 gaagtacgtc cagaacctgg acgtctgagg gggcacagga ccgatggcac aggaggggct
     6901 gttcgccggg cgggtcgagc ccgtccagct cgaggaggag atccagcggt cctacctcga
     6961 ctacgcgatg tccgtcatcg tgggccgggc gttgccggag gtccgcgacg gcctcaagcc
     7021 tgtccaccgg cggatcctct ggtcgatgct cgaatcgggc ctccgacccg accgcccgca
     7081 ccgcaagagc gtcgcggcgg tgggcgaggt catgaagaag taccacccgc acggggatgc
     7141 accgatctac gacgcgttgg tgcgcatggc gcaggacttc tcgctcaggc accctttggt
     7201 ggatccgcac gggaacttcg ggtcggtcga cggcgaccca ccggcgtcga tgcgctacac
     7261 ggaggcgcgc ctcgcgccga tcgcgatgga gctgctccga gacatcgact ccgacaccgt
     7321 ggacttcgtc ccgaacttcg acggctacga gcaacagccc gtggtgctcc cggcgcgctt
     7381 ccccaacctc ctggtcaacg gctcgggcgg tatcgcggtc ggcatggcga cgaacatccc
     7441 gccgcacaat ctcggcgagg tgatcgatgc cgtgatccat acgctcgacc atcctgaggc
     7501 cacgccgaag gagctcatga agttcgtcaa aggacctgac ttcccgacgg gcgccgtcat
     7561 catgggccgc gacggcatcc gggacgcgta cgagaccggg cgcggctcga tcaagatccg
     7621 cgcggtgtgc tcggtcgagg aggggtcggg cggccgggct cgcatcgtcg tgaccgagct
     7681 gccctaccag gtgaacaagg cgcgcctcgc agagaagatc gctgagttgg tacggagcgg
     7741 caaggtgagg gagatcgccg acctcaagga ccactcctcc gggcgggcgg ggatgcggct
     7801 cgtgatcgac ctgaagcgcg gcgccaaccc ccaggtcgtg ctcaaccagc tctacaaaca
     7861 cacgcagctg caggagtcgt tcggcgtgat catgctggcg ctcgtcgacg ggatcccacg
     7921 cacgctgaac ctggccgagg cgatcgggta ctacgtggac caccaggttg acgtcgtcac
     7981 gcgccgagcc cggttcgatc tccgcaaggc ggaggaccgc gatcacatcg tccagggcct
     8041 gctgatcgcc ctcgaccacc tcgacgaggt gatcaagatc atccgagcgt cccaggatgc
     8101 cgagcgggcg cgcacgaaac tgatgagcac gttcaagctg tcggagatcc aggcgaacca
     8161 catcttggat atgccgctgc gtcggctcac caagctcgcg cgcaccgagc tcgagcagga
     8221 gcacaaggag cttctcgaca ggatcaagta cctcaaggcc ctcctgaagg atccgaagaa
     8281 gatccgcggc gtgatcaagg aggagatcct cgagatcaag aagaagcacg cggacccgcg
     8341 gcgtacgcag atccgggcgg acgagggctc gttcgacatc gaggacctga tcgcggaaga
     8401 ggacgtcgtc atcacggtca gccgggccgg ctacgtgaag cgcgtcccga tcgacacgtt
     8461 ccgacggcag gggcgcggcg gcaagggggt ccgcggggcc acactgaagg aggaggacgt
     8521 cgtcacgaac gtcttcacca ccaccaccca ccactggctg ttgttcttca ccacgaaggg
     8581 caaggtgtat cgggtgaagg tgcacgaggt cccggattct tcgcgcacgg cccgggggct
     8641 ctacgccgcg aacctccccg gagtggcgat cgccggcgac gagcgcgtca gcgccgtgct
     8701 cgacatgaag ctgtacgaag acggccgcta cctgctgttc gccaccaaga aggggatggt
     8761 gaagaagacg ccgctccccg aatatgcgtc gtcgcggacc ggcctggccg cgatcaacct
     8821 gaagcccggc gacgagctga tcgacgtcaa gctcacggac ggcaaggacg aggtcttcct
     8881 ggtctcgcgg aagggacagg cgatccggtt caaggagccg ctggttcgcc ccatgggtcg
     8941 atccgccgcg ggcgtgatcg ggatgcgcct ggccgaggac gacgaggtga tctccctcag
     9001 cctctcctcc gacggcgagg agatgatcag tgtctcgcag caggggtacg gcaagcgctc
     9061 gccgctgagg gactacccgt tgaaggggcg tagcggcaag ggcgtcatcg gacaccagct
     9121 cacgaagaag accggccagc tcgctggcgc gttcgtcgga tcgaaggaac aggacatgtt
     9181 cgtgatctcc tccagcggta tcgtgatccg cgtgcctgcg agtgagatcc gccgtgtcgg
     9241 tcgttcgagc cagggcgttc gcacgatgcg cgttgaagag ggggcgcacg tcgtcgccct
     9301 tgcacccgtg atcatgcaga tggaggagta gcgcactccg agagacggat gcggaaggga
     9361 accgcagcgc atcgccgggc gttactgcga tgatgtcgga actcccatga acatcggact
     9421 cggcttgctc gtcgaagctg ccgcgttcct ggcggccgtc tacgtcgtgg cgcagttgcg
     9481 gccgcgtctc ccgcggcaga ccgcggatct cgtgctcggg tcgatcggct gcgtgatggg
     9541 cgccggcgcc ctgctgttcc agagggacgt gagtctcgtc ggtggtgtgc tcgcgcctct
     9601 gttcgcggcg gtcatcgtgg tggggcacgt gcgcgggttg gacctcatgg gtgtccggct
     9661 gccggcctgg gtgcaaatcc ccgcgctggg gcgccgaggg gcgtacgcta tcgacccagc
     9721 cgagacccga acgatcgagg agcctgtgca gcaaccacgg tcagcgacgg agcctgagcc
     9781 ggcatccggg ttcgatcccg agccgttgcc cgacgacgag cccttcccgc gcgaggatga
     9841 gctcgtccct ccactcggcc cggtggcccc ggaagcgacg tggccctcca cgatcgtccg
     9901 gccgcccgcc gagacgcggc tgccgcggcg cggtgtgcgc aggacacgcg tggagatcag
     9961 acgactcggc ccgctgtcgg tgctgaagtt ctcgctgatc ttctacttct gcatcttcct
    10021 cgtgatctat ctctcgctcg cgatcatctg gggcatcctg tccgcgagcg gcgcgatcga
    10081 ctcgctggag gagctgctcg gcgtcatctt cccgtcggcc ggcgcccagt cgccaaccgg
    10141 cgagctgagc accggcgatg cgccgcccct cgagatcgtc acgggccagc tcttcacctg
    10201 gctcttcatc ggcggatgcg tcggcgtagc gatctggtcc tgtatcaacg tgttcgtggc
    10261 gatcatgtac aacctgatca gcgacatcgt cggagggatc gaggtcacgc tggcagaccg
    10321 gcgcgactga ggccgtctgg gacggtgacc tcacccggga gcccgcgaag tggtttcgat
    10381 tgacaccgtt tccggaccgt gactaccctg cggcgagggg ctcgcggccc tcgggcctgt
    10441 agctcagctc ggttagagcg cttccctgat aaggaagagg tcgatggttc aagtccatcc
    10501 aggcccacgt tcttggccag gcccactctg tccacccact cgataggagg ctgtgcgcga
    10561 tgggagacga caccgaaggc acggagaaga agggcggttt cttccggaag ctcctgaagc
    10621 tcgccttcat cggggccatc gtggccgccg tcatgcagtt catgaagcgc cgccgcggtg
    10681 aggacgtcga cgacgtggag tggcaggagc tgccgccgcc cgcgggcggg tagcccacct
    10741 gatctccgac gtggaaggcc cgccacacgg cgggccttct ccgtgtccga agctctcgct
    10801 ctagactcgc ccgcgtgctc gagcccggaa cgccctggga tctcgcccac acggcggccc
    10861 gggcggcgaa cgtgaagttg cgccccctga cctcgctggc gcagttcgac cagacactcg
    10921 aggtcatggt ggccacgtgg ggcgcgttcc agctcccgcc tcgcgagatg atgacggcgc
    10981 tcgcccacag cgggaacgtt ccgttcgggg cctacgacgg cgatcgcctg gtgggattcg
    11041 tcctcggatg ggcaggcgtg gatcacggcg ggctccacgt ccattcccac atgctggccg
    11101 cgctccccga tcggcgccat cggggggtcg gctacgcgtt gaaactcggg cagcgcgccc
    11161 aggcgctcga gcaggacatc cacgtggcgc gctggacctt cgacccgctg gtggcacgca
    11221 acgcgtggct caaccttggc aagctcggcg cggtggcgga cggcttcggc cgggcgttct
    11281 acg
//
LOCUS       DASUYD010000160         5332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1631244, whole genome shotgun sequence.
ACCESSION   DASUYD010000160 DASUYD010000000
VERSION     DASUYD010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5332
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1631244"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF195_09250"
     CDS             <1..379
                     /locus_tag="VF195_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX6581044.1"
                     /translation="IKRVALATEFHPARFEQHRRITSHRSVTASFNCRPVVTPLGPGD
                     DYSQHAYGLAVDVNPLQNPYVTTDGFVRNRVAEPYTVRSRTLEGMIHEGDVVVRSFAA
                     IGWEWGGNWSGDKDYMHFSQSGT"
     gene            519..947
                     /locus_tag="VF195_09255"
     CDS             519..947
                     /locus_tag="VF195_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009205019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX6581045.1"
                     /translation="MKWVTRARPKTDRIACPWLIRRFIDSDAEILYVPADQVLDVADR
                     EAARSFDAPGAEFTHRNGKCSFEVLIDEFGLGKNPALARLARIVHAADVSEDRESDAL
                     GAGLEAIGAGGVLAEADDQVLLEKGSFVYDALYAWCEQHV"
     gene            1030..1197
                     /locus_tag="VF195_09260"
     CDS             1030..1197
                     /locus_tag="VF195_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581046.1"
                     /translation="MSPANVLAPITGKTFHRKRHSPTNPTAATIVANQSTLDHTGEGT
                     SNRPSNSVVLT"
     gene            complement(1258..1623)
                     /locus_tag="VF195_09265"
                     /pseudo
     CDS             complement(1258..1623)
                     /locus_tag="VF195_09265"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
     gene            complement(1554..2159)
                     /locus_tag="VF195_09270"
     CDS             complement(1554..2159)
                     /locus_tag="VF195_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6581047.1"
                     /translation="MYLGWLMHRVKGGLLAGVMFVLPSVFIMFGLSWLFAAHGDVTWV
                     NAIFAGLAPAVIALVAWATIRIGSKSLANGLMLAVAIVAFLAIFVVHVPFPLIVLAAL
                     VIGFVGGAVRPDLFDVGVAEELEEQERIALRDDAPRPPHTVPTRARALKTLVVGLVVW
                     LVPSRLSPPGAARTTPSPRWAGSSLVRRSSRSAGRTRSWPT"
     gene            complement(2041..2304)
                     /locus_tag="VF195_09275"
     CDS             complement(2041..2304)
                     /locus_tag="VF195_09275"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX6581048.1"
                     /translation="MNFGGPAGQIAIMHEEIVDRRKWVSNARFLHALQLLHAAAGAGG
                     AAASDLPRMVDASRQRGASRGRHVRAAQRIHHVRPELALCGPR"
     gene            complement(2736..3005)
                     /locus_tag="VF195_09280"
     CDS             complement(2736..3005)
                     /locus_tag="VF195_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581049.1"
                     /translation="MPQIIVTAAVNDVEEWLKFKAEMVPSMSAVASDGASYVAIDGSN
                     QVASTWDVPDMEAFQAVQSSFSPELAAAAERAGMIPSTMVVYIKK"
     gene            complement(3503..4375)
                     /locus_tag="VF195_09285"
     CDS             complement(3503..4375)
                     /locus_tag="VF195_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581050.1"
                     /translation="MNDTDVRDLLARVADEVPATPVDPEPLLRRGYRRMARTAVAGAL
                     GIACAIVVAVGGVGLIRSSAPTTMPADEGQPTGPPETVTGETAGTSTSPCFDSQDGPP
                     GQVCLGPLDEGTYTSQRFEPALTFTVPAGWNNPWDTRGAFTLWTPGWSPGNTLFMEPG
                     LHVRRDPQPYEGCVDAVDTTIGTSAIELATWVANHPAIATELPSPVEVGGLSGYQLDV
                     SLADTWQKSCRERAPGAAVWLFEDLYLNENPGFTTMRLILLDLPDGSVLIAIDGTQVD
                     AAMPVVRSFTFDLN"
     gene            complement(4372..4905)
                     /locus_tag="VF195_09290"
     CDS             complement(4372..4905)
                     /locus_tag="VF195_09290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6581051.1"
                     /translation="MEHDRTGVVSAVDRKAGLRSRLADAYVRSAPGGIRLAYLLTGDR
                     AVAEDLVQEAFVRFVGRLRFLRDPDAFEPYLRRTIVNLSKNHFRRRAVERAYLEREGR
                     RIEDVSTDPDVATSDTLRSALLRLPLRQRTALVLRYFEDLPDATIAEILGCRQATVRS
                     LVARGLEALRTTPEVIT"
     gene            complement(5113..>5332)
                     /locus_tag="VF195_09295"
     CDS             complement(5113..>5332)
                     /locus_tag="VF195_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006378515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX6581052.1"
                     /translation="GQDVRIGGGPTTLRDFLAAGLVDHAHIVVVPILLGRGVRLWDGL
                     ESLEKDYQVEATSSPSGVTHVMFTRTAL"
BASE COUNT          951 a         1789 c         1782 g          810 t
ORIGIN      
        1 catcaaacgg gtcgcgctcg cgaccgagtt ccaccccgct cggttcgagc agcatcggcg
       61 gatcaccagc catcggagcg tgaccgcgtc gttcaactgc cgtccggtgg tgaccccgct
      121 cggcccgggc gacgactact cccagcatgc gtacggtctc gccgtcgacg tcaacccgct
      181 ccagaacccg tacgtgacca ccgacggctt cgtacgcaat cgcgtggccg agccgtacac
      241 ggtccggagc cggaccctcg aagggatgat ccacgagggc gacgtcgtcg tccgctcgtt
      301 cgccgcgatc ggctgggaat ggggcgggaa ctggagcggc gacaaggact acatgcactt
      361 cagccaatcg ggaacgtagc aggtcagagg ccgtatttcc atccggaatg ggcacaccgt
      421 gtgcccagag cgaaagaggc ggcatggtcg cgtgcacctg ctgccgtccg gaaggcgcga
      481 ggttgcgcgt tcatcgggca tgctggagat tcgctcccgt gaagtgggtc acgcgcgcac
      541 ggcccaagac cgaccggatc gcctgccctt ggttgatccg ccgattcatc gactcagacg
      601 ccgagattct ctacgtgcca gctgaccagg tcctggacgt agccgatcgg gaggctgcgc
      661 ggtccttcga tgcgcctggc gctgagttca cccaccggaa cgggaagtgc tcgttcgagg
      721 tcctaatcga tgagttcggg ctgggcaaga atcccgctct tgctcgcctt gcccgaatcg
      781 tccatgccgc cgacgtctcg gaggaccgag agtccgacgc gcttggagct ggactcgaag
      841 caatcggcgc ggggggcgtc ctcgcggagg ccgatgacca ggtcctcctg gagaagggct
      901 cgttcgtcta cgacgccctc tacgcgtggt gcgagcaaca cgtctagacg aacgggcagc
      961 acccgcgacg ctagagccga caacccaacc tggcgtcgca gaggttaatg cccaaacaac
     1021 ccgtaccaga tgagccccgc caacgtgctc gccccgatca ccggcaagac cttccaccgg
     1081 aagcgccaca gtccgacgaa cccgaccgcc gcgacgatcg tcgcgaacca gtcgacactc
     1141 gaccacacgg gagaggggac ctcgaaccgg ccgagcaact cggtcgtgct gacctgatcg
     1201 aaagagcgtg gagatggcga acgtgaccgt aaggttggcg attaccccga cgaccgccgc
     1261 ggtcacggtc tgaagggccg ctccgaggcg tcggttcccg cgcaggctct cgacgtacgg
     1321 ggctccgagg aagatccaca ggaagcaggg tgcgaacgtg gcccaaacgg ccacgaacgc
     1381 gccgagcgtg ccggccacga gtgggtcgag atcgccgggg ttctgatacg cgcctacgaa
     1441 tccgacgaac tccaagacca tgatcagcgg tccaggcgtg ctctcggcga gaccgaggcc
     1501 gacgaccatc tgacccggca agagccaccc gtaggtctgc acggcggcga cgttcacgta
     1561 ggccaggacc gcgtacgccc cgccgaacgt gatgagcgcc gcacgagaga agaaccagcc
     1621 catctcggtg agggtgtcgt ccgggccgcg ccaggcggcg ataagcgcga tgggacgagc
     1681 cagacgacga gcccgacgac gagcgtcttc agggctcggg cgcgcgtcgg caccgtgtgg
     1741 ggaggacggg gcgcatcatc tcggagcgcg atccgctcct gctcttccag ctcctcggcc
     1801 actcccacgt cgaagaggtc aggccggacc gctccaccga cgaagccgat gaccaaggcc
     1861 gccagcacga tgagggggaa ggggacgtgc acgacgaaga tcgcgaggaa ggcgacgatc
     1921 gccaccgcga gcatcagccc gttcgcaagc gacttgctcc cgatgcggat cgtcgcccat
     1981 gcaacgagtg cgatcacggc cggagccagc ccggcgaaga tcgcgttgac ccacgtcacg
     2041 tcaccgtggg ccgcaaagag ccagctcagg ccgaacatga tgaatacgct gggcagcacg
     2101 aacatgacgc ccgcgagaag cccccctttg acgcgatgca tcaaccatcc gaggtagatc
     2161 gctagctgct gcgcctccgg ccccggcagc agcatgcaga agttgaagtg catgcagaaa
     2221 ccgggcgttc gagacccact tccgtcgatc gacgatctcc tcatgcatga tggcgatctg
     2281 gccggcaggg cctccgaagt tgatgaaacc gagcttggtc cagaaacgag cggcctcgcg
     2341 gaaggggacg tatgtaggga cggctcaggc gaggtcatgc gcgaacccta gtcgggaccg
     2401 cggccccgct ctccagcggc attagagggg cgctaagaaa attggtctgc acccgcgagc
     2461 cgtccggaag tcgcgcgtcc acccaaggcg acgttgcgtc gacccggagg ccgagcggga
     2521 ccacgatccg ttcgatcacg tggagggccg cctcctcccc ctcaaacaga cggtccgcca
     2581 ccctctcgat ccgcctacaa ggctccttgt gcgaacaccg gggttgcctg gcctcgaggg
     2641 ggtggaccat tcgcggatgg agccatggat cgggtcgcac aagcaacgct ccagagtgcg
     2701 cgtacgctcg cctgtgcgtc gcggacgcgg ccggtctact tcttgatgta cacgaccatc
     2761 gtcgatggga tcatcccggc tcgctccgcc gccgccgcca gttccggaga gaaggagctc
     2821 tgaactgcct ggaaggcctc catgtcaggg acgtcccacg tgctagcgac ctggttgctg
     2881 ccatctatgg cgacgtagct ggccccgtcc gacgcgaccg ccgacatcga ggggaccatc
     2941 tctgccttga acttcaacca ctcctcgaca tcgttgaccg ccgcggtgac gatgatctgg
     3001 ggcatgacaa gtctccttcg ggacgccgac cggctcaggc ggcgagtata gagaccctgg
     3061 agttcgtacg gacgttcccc ttcccccgtg agggcgacta gcttgtcccg ttaggagccg
     3121 ccggacatcg ggacgttttc gtctgactcc tgacgcttcc tccggtcagg acgtctggac
     3181 acaagctacg cactctcaag ataaggagcg acaagcgagt gtcgtgctcg ccatcgaaga
     3241 acaactgacc cccgctacgt gaagctccag cggtggttcg aagacgaaac cgttcgcctc
     3301 atggcgccgc ctggtcccag tcgtccccgc gttcgccccg cccggccgca gcgaggctga
     3361 ccgtgctggg gcgccgggca cgagacaaag gggtgccaag aaatctggtc tgcacctgcc
     3421 tcccggcgag tgctcggttg cggctccgct tcgtcgctcc cttccaccag aggcggtccc
     3481 tgcagaagaa cgcgccgggg cctcagttca ggtcgaacgt gaacgagcgc acgaccggca
     3541 tcgcggcatc cacctgcgtc ccgtcgatcg cgatcaggac gctcccgtcc ggcagatcga
     3601 gcaggatcag ccgcatcgtg gtgaatccgg gattttcgtt gaggtagaga tcctcgaaca
     3661 gccacaccgc ggcccctgga gcccgctcac gacagctctt ctgccacgtg tccgcgagcg
     3721 acacgtcgag ctggtagccg gagagcccgc cgacctccac gggcgacggt agctccgtcg
     3781 cgatcgccgg atggttggcc acccaggtcg cgagctcgat cgcggaggtg ccgatcgtgg
     3841 tatcgacggc atcgacgcac ccctcatagg gttgcggatc gcggcggacg tgcaggcccg
     3901 gctccataaa cagggtgttg cccggggacc acccaggggt ccacagggtg aacgcgccgc
     3961 gcgtgtccca cgggttgttc catccggccg ggacggtgaa ggtgagggcg ggctcgaagc
     4021 gctgcgacgt gtacgtcccc tcgtcgagcg gtccgaggca cacctggccc ggtggtccgt
     4081 cctgcgaatc aaaacacggg ctggtcgacg tgccggccgt ctcgccggtg accgtctccg
     4141 gcggcccggt cggctgtccc tcatcggccg gcatcgtcgt cggcgcgctc gaacggatca
     4201 ggcccacgcc cccgacggcg acgacgatcg cgcaggcgat ccccagcgcg ccggcgacgg
     4261 ccgtccttgc catccgacgg tagccgcgac ggagcagcgg ctccggatcc acgggcgtgg
     4321 ccggcacctc gtcggcgacg cgcgcgagca ggtctcggac gtccgtgtcg ttcatgtgat
     4381 cacctctgga gtcgtgcgaa gtgcctcgag accacgggcg acgagcgagc ggaccgtggc
     4441 ctggcggcag ccgaggattt cggcgatcgt ggcgtccggg aggtcctcga agtagcggag
     4501 gacgagcgcc gtgcgctggc ggagcgggag ccggaggagc gccgagcgga gcgtgtcgga
     4561 cgtggcgacg tccggatcgg tgctgacgtc ctcgatcctc cggccctcgc gctccaagta
     4621 cgcgcgctcg accgctcggc gccggaagtg gttcttcgac aggttgacga tcgtgcgtcg
     4681 caggtatggc tcgaaggcgt ccgggtcgcg taggaaccga agccgaccca cgaagcggac
     4741 gaacgcctct tggacgaggt cctcagcaac ggcccggtcg ccggtcagca ggtacgcgag
     4801 ccggatgcct ccgggcgccg accgcacgta cgcgtcggcg agccggctgc ggagacccgc
     4861 ctttcgatcc acggccgata ccaccccggt ccgatcgtgc tccatgtcct tctgacacgc
     4921 gagcgcgtcg ttctgtggca gcccggtggc aactctctgg cgagcacgaa ctgaagggtg
     4981 tccccgaccg ctggcacctc taccgggtcc gggggtaggc ggtccggccg aaaaggggcg
     5041 caaagaaatc gggtctgggc ggcggtgccg ttgaccccgc atgcgcccct cgggctttgt
     5101 cgaccaggcg ggtcagagag cggtacgggt gaacatcacg tgcgtgactc cgctgggtga
     5161 ggaggtggcc tcgacctggt agtccttctc gaggctctcc agtccgtccc agaggcgcac
     5221 gcctcggccg agcaggatcg ggaccaccac gatgtgcgcg tggtcgacga gcccggcggc
     5281 aaggaagtcg cggagcgtgg ttggacctcc accgatgcgt acgtcctggc cg
//
LOCUS       DASUYD010000161        12413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1412291, whole genome shotgun sequence.
ACCESSION   DASUYD010000161 DASUYD010000000
VERSION     DASUYD010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12413
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1412291"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..747)
                     /gene="hisA"
                     /locus_tag="VF195_09300"
     CDS             complement(46..747)
                     /gene="hisA"
                     /locus_tag="VF195_09300"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEX6581053.1"
                     /translation="MIVIPAIDLRRGRCVRLSRGDVDAETVYAEEPAEVARRFEADGA
                     RRVHVVDLDAARGEGSNRGVVKDICRRVAVPVQLGGGLRTLAAVETALEDGAARAILG
                     TAAALDPRFVSQCVDRVADAVVVAVDVRDGRVMTHGWQELGPAVEWAVRTLEHAGAPR
                     FLVTSIARDGTMEGPDLALYERVLELTDRPVIASGGIRAVADVWALRDLGLEGCVVGK
                     AMYSGGLRMREVVRG"
     gene            complement(744..1346)
                     /gene="hisH"
                     /locus_tag="VF195_09305"
     CDS             complement(744..1346)
                     /gene="hisH"
                     /locus_tag="VF195_09305"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX6581054.1"
                     /translation="MIAILDYGMGNLRSVARAIEHVGGKPEVTEDALSALGADGMVVP
                     GVGHFGACVRSLRDRGLDRVVIEFASSGRPMFAVCVGMQILFEGSEEDAEAGLGLFEG
                     VSRRLPDEVKVPHMGWNSVRWTGRHPYVREVPTGTLFYFVHSYAPGVAVGTTVGATEH
                     GRPFAAAVARDNVFATQFHPEKSGEPGLRLYEAFVLEAGS"
     gene            complement(1346..1927)
                     /gene="hisB"
                     /locus_tag="VF195_09310"
     CDS             complement(1346..1927)
                     /gene="hisB"
                     /locus_tag="VF195_09310"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006544426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX6581055.1"
                     /translation="MTRAATVVRETNETTVRVELELDGVGSATADTGLPFFDHILQQL
                     GAHAGFVLTVTAKGDLEVDGHHTVEDVGLALGQALKEALDDKQGIRRFASTTVPLDEA
                     AVEVALDLSGRPFVVHEVDVAAETIGTYDTGLTEDFVRAFATAAEMTIHVRLVAGRSP
                     HHVVEAEFKAIATCLRDAVRVHGEGVPSTKGTL"
     gene            complement(1944..2987)
                     /gene="hisC"
                     /locus_tag="VF195_09315"
     CDS             complement(1944..2987)
                     /gene="hisC"
                     /locus_tag="VF195_09315"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX6581056.1"
                     /translation="MIAPRPGLRDIAPYESPQVDVHARLNTNECPYPLPDGFADDLAK
                     AVRDLSLNRYPDREAAALRAGLADVADHPFEGTWAANGSNEILLQLLQSYGGPGRRVV
                     VFEPTYALHSRLAWVTRSDVVAVRLEPPWRIDARHVAEAWAADPSVVFVCSPNNPTGN
                     VQTEETIRELVARQPTLVVVDEAYIEFGGDSVASLVRRHANLAVVRTLSKAFALAGAR
                     VGYCLASRDLVDDLRRVALPYHLSSLNQAAGVVALAHSHDAVRILDAVREQRDRILAT
                     LPSMGAEAFPSSANFVLFRPPRHAREVWRGLLDRGVLVRDFTDLVPECLRVTAGTPEE
                     VDRFLSALEEVLA"
     gene            complement(3163..3414)
                     /locus_tag="VF195_09320"
     CDS             complement(3163..3414)
                     /locus_tag="VF195_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HEX6581057.1"
                     /translation="MLRSATDATTVVEVEGATVREALTAAIEIHPDLQARLFDEHGQL
                     RRFLNVFVDEEDIRFERGLETPVAAGQTVSLLPAVAGGR"
     gene            complement(3473..4453)
                     /gene="thrC"
                     /locus_tag="VF195_09325"
                     /pseudo
     CDS             complement(3473..4453)
                     /gene="thrC"
                     /locus_tag="VF195_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017305309.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
     gene            4557..5195
                     /locus_tag="VF195_09330"
     CDS             4557..5195
                     /locus_tag="VF195_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX6581058.1"
                     /translation="MSNTKDYLVPVVVEQTSRGERSFDIYSRLLKERIVFLGTPIDDN
                     VGNLIMAQLLHLESEDPDKDISLYINSPGGDITSLFAVYDTMQYVKPDVSTIVMGQAA
                     SAAAVLVLAGTKGKRYALPHSRILLHQPHGGAEGQAVDIEIQAREITRYRKLLEELIA
                     FHTGQPLERVSKDTDRDFILTAEGAVEYGVVDEVITTRKITPELAAVGGGGS"
     gene            complement(5995..6918)
                     /locus_tag="VF195_09335"
     CDS             complement(5995..6918)
                     /locus_tag="VF195_09335"
                     /inference="COORDINATES: protein motif:HMM:NF018755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1353 domain-containing protein"
                     /protein_id="HEX6581059.1"
                     /translation="MEVDEQALSQLQDLAVRSGHPLPRLVHDAVDSLHRSLVGSSSIQ
                     LHLPFKKRWSELDAEILLRQVSATGFELDEPFRFEEPGHQDIVVRRGDATDLASVPSF
                     LTWLVPRYGRHTLPALLHDQLVVGGMDPNDREKADATFRDAMGGTRVPLVMRWVMWAA
                     ISLATHLLRAWRWKVLIAAWVALYGLAGLDSLLAVLGWRPVSALSSAAVLALIWVSPV
                     LLSVIWGRRYRFGLIVAYSLLVLPASVISVAATIILYRLLEVVAQVALLLMRRRGASV
                     QVNPVELSNLEYTQRGEVPPGMPPPEQMTGT"
     gene            complement(7113..7412)
                     /locus_tag="VF195_09340"
     CDS             complement(7113..7412)
                     /locus_tag="VF195_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581060.1"
                     /translation="MNEPVAVVGGPVAAAGVERTTKTSGMALKSANTARRAFIAASLS
                     TEPPTPMRASGLSKTGGSLYRIGLNHSNDDDLAIDFVTILIFSSHFVASAARESD"
     gene            complement(7846..8718)
                     /locus_tag="VF195_09345"
     CDS             complement(7846..8718)
                     /locus_tag="VF195_09345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017154581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX6581061.1"
                     /translation="MPRMHGDELEIDEPLVRRLLADEFPEWVSLPLRRVEPAGTDNAI
                     FRLGEELAVRLARRKGPTRSGGKEFDWLPRLAPLVPLEIPVPVAQGRPTDEYPWFWDV
                     FTWVDGAPTPIEAIDPTFAARDLALFVAALQGVDPSGAPMGRGIPLAERDEEIRYWLA
                     RFDGDPAVVGEWERALAAPAWQGPPVWHHGDLDVRNWLVRDGRICAVIDWEAMGVGDP
                     ACDVMVAWKLHSGEARDAFREALPTDDATWERARGWALSQAVAALAYYTPENNPTLYR
                     EAEAWLELVLSERG"
     gene            8927..9244
                     /locus_tag="VF195_09350"
     CDS             8927..9244
                     /locus_tag="VF195_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581062.1"
                     /translation="MNTNVAHGGAVLGHQLKVLRRHVRRPRFRPFDRVFLAAAAHVLP
                     RDTWASFLVTLQTLLRWHRELVRRKWTYRTASKPDNESGRPHRPLSLSLSPSAFSLGR
                     LSC"
     gene            complement(9241..9714)
                     /locus_tag="VF195_09355"
     CDS             complement(9241..9714)
                     /locus_tag="VF195_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581063.1"
                     /translation="MKRFAVVTVTTLAVLGLLAQQALADSPHFVQGPTTSVTVSGNEV
                     DLNLSFKAAGLGNATAYATWSLDGSGTLFSRCYNRGGNKPQADNKQETVDIGATFDTA
                     VNHGNTTFNGTVATVTSTLTCPGNQVVRIESFSATGTLSLVGGTLTADLSWTFPS"
     gene            10239..10712
                     /locus_tag="VF195_09360"
     CDS             10239..10712
                     /locus_tag="VF195_09360"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6581064.1"
                     /translation="MAAHLPRRVLTAPATTGVSHQLMSTAGALGDLATALSIEWDGID
                     DDARIALVRKIQQLALHLGHLAERSGRPSLEPTDRQSGSPWFDGLSFLTPREHEILRA
                     LALGASTGRIGERFGIRPATVRSHVKSLLVKLGVHSRIEAVSVFLKWDSRQEPPV"
     gene            complement(11187..11837)
                     /locus_tag="VF195_09365"
     CDS             complement(11187..11837)
                     /locus_tag="VF195_09365"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009942573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX6581065.1"
                     /translation="MRLVDLSHVIRHGMVTYPGLPGPEIGEHLSWEASRERYAPGTEI
                     RIAQISMVANTGTYLDTPAHRYRDGWGIDDLPLEMVAAVPGIVVDDTGPEIGPDALKG
                     LEVAQRAVLVRTGWSRHWGTDRYGEQGHPYLTAEAAEALVAGGATLVGIDSVNIDGTH
                     TGDRPVHTILLAAEIPIIEHLTGLETLPAEGFDLFAVPQRVEGMGTFPVRAFASVR"
BASE COUNT         2098 a         4280 c         4004 g         2031 t
ORIGIN      
        1 gtccggcacc gacgtccagg cacggcacga tgcgcttggc gagggtcatc cgcggacgac
       61 ttcccgcatg cgcagccccc ccgagtacat ggccttgccg acgacgcacc cctcgaggcc
      121 gaggtcccgc agggcccaca cgtcggcgac cgcgcggatc ccgccgctcg cgatcaccgg
      181 gcggtcggtg agctcgagca cgcgctcgta cagggcgagg tcgggaccct ccatcgtccc
      241 gtctcgcgcg atggacgtga cgaggaacct gggcgccccc gcgtgctcga gtgtgcggac
      301 ggcccactcg accgccggcc ccagctcctg ccacccgtgc gtcatcacgc gcccatcccg
      361 cacgtcgacc gcgaccacca ccgcgtccgc cactcgatcg acgcactggg acacgaagcg
      421 cggatcgagt gccgcggccg tcccgaggat cgcgcgcgcg gcaccgtcct cgagggccgt
      481 ctcgacggcg gcgagcgtcc ggagaccgcc gccgagttgc accggcacgg caacacgccg
      541 gcagatgtcc ttcacgacgc cgcggttcga cccctcgccc cgcgccgcgt ccaggtccac
      601 gacgtggacg cgccgggcgc cgtccgcctc gaagcgccga gccacctccg ccggctcctc
      661 cgcgtagacg gtttcggcat cgacgtcgcc gcgagacagg cggacgcagc gcccccttcg
      721 gaggtcgatc gcggggatga cgatcacgac ccggcctcga gtacgaacgc ctcgtacagc
      781 cgcaggccgg gctcgcccga cttctccgga tggaactgcg tggcgaacac gttgtcacgc
      841 gccacggcgg cggcgaacgg gcggccgtgt tcggtcgcgc ccacggtggt cccgacggcc
      901 accccgggcg cgtacgagtg gacgaagtag aagagggtgc ccgtcgggac ctcgcgaacg
      961 tacggatgtc gccccgtcca gcgcaccgag ttccagccca tgtgcggcac cttcacctcg
     1021 tccggcaacc gccgcgagac gccttcgaac aggccgaggc ccgcctccgc atcctcctcg
     1081 ctgccctcga acaggatctg catgccgacg cagacggcga acatgggccg gccgctcgac
     1141 gcgaactcga tcaccacccg gtccagcccg cgatcccgaa ggctccgcac gcacgcgccg
     1201 aagtgcccga ccccggggac caccatgccg tcggccccaa gcgccgacaa agcatcctcg
     1261 gtcacctccg gcttgccccc gacgtgctcg atcgcccgcg cgacgctgcg gaggttgccc
     1321 atgccgtagt cgaggatcgc gatcatcaca gcgtcccctt cgtactcggg acgccctcgc
     1381 cgtggacgcg aaccgcgtca cgcaggcacg tcgcgatagc cttgaactcg gcctccacga
     1441 cgtggtgggg agagcgcccg gccacgaggc gcacgtggat cgtcatctcg gcggcggtcg
     1501 cgaacgcgcg cacgaagtcc tccgtcaggc cggtgtcgta ggtgccgatc gtctcggccg
     1561 caacgtcgac ctcgtggacg acgaacggcc gccccgacag atcgagcgcg acctcgacgg
     1621 ccgcctcgtc gagcggcacc gtggtcgagg cgaagcgccg gatcccttgc ttgtcgtcga
     1681 gcgcctcctt caacgcttgc ccgagcgcga ggccgacgtc ctcgacggtg tggtggccgt
     1741 cgacctcgag atctcccttg gcggtcaccg tgaggacgaa cccggcgtgc gccccgagct
     1801 gctgcaggat gtggtcgaag aacgggagcc ccgtgtcggc ggtcgccgat ccgacgccgt
     1861 ccagctcgag ctcgacgcgg acggtcgttt cgttcgtctc gcggaccacc gtggccgcgc
     1921 gcgtcatgcc gggacctcct cgctcatgcg aggacctcct cgagggccga caggaaccgg
     1981 tcgacctcct ccggcgtgcc ggcggttacg cgaaggcatt ccggcactag gtcggtgaag
     2041 tcgcggacga ggacgcctcg gtcgagcagg ccccgccaga cctccctggc atgccttggc
     2101 ggtcggaaca gcacgaagtt cgccgacgac gggaaggcct cggcgcccat cgacggcagc
     2161 gtcgccagga tccggtcgcg ctgctcccgg acggcgtcca agatgcggac cgcgtcatgg
     2221 gaatgggcga gggctacgac ccccgccgcc tggttgagcg agctcaggtg gtacgggagg
     2281 gcaacgcgcc ggaggtcgtc gacaaggtcg cgggacgcga ggcagtagcc gacgcgagcg
     2341 ccggcgagag cgaatgcctt cgagagcgtt cggacgaccg ccaggttcgc gtgccgtcga
     2401 acgagcgatg cgaccgagtc cccgccgaac tcgatgtatg cctcgtcgac gacgaccagc
     2461 gtgggctggc gggcaacgag ctcgcggatg gtctcctcgg tctggacgtt cccggtgggg
     2521 ttgttcggtg agcagacaaa caccaccgat ggatcggccg cccacgcctc ggcgacgtgg
     2581 cgagcgtcga tcctccacgg cggctcgagc cgtaccgcta ccacgtccga acgggtgacc
     2641 catgccaggc gcgagtgcag cgcgtacgtc ggctcgaaga cgacgacgcg tcggcccggg
     2701 ccgccgtatg actggagcag ctgcagcagg atctcgttgg agccgttggc cgcccacgtt
     2761 ccctcgaacg ggtgatcggc gacgtcggcg agcccggcgc gcagcgccgc cgcctcccgg
     2821 tctgggtatc ggttcagcga gaggtcgcga acagcctttg ccaggtcgtc ggcgaacccg
     2881 tccggcagcg ggtaggggca ctcgttggtg ttcaggcggg catggacgtc gacctggggc
     2941 gactcgtagg gcgcgatgtc gcgcagtccg gggcgggggg cgatcacgac ctggtcgtcc
     3001 gctcatcggt ccggacctcg acggcccgcg aggccgggga acgtagcatc tgcacatgat
     3061 gacacggccc catacaggct ctggtcgcgg cgtgggagac agatgcccag gctagcctgt
     3121 gctcagatgc agccagacgc cggtcacata tcgttcgcgt cgctatctcc cgccggccac
     3181 cgccgggaga agcgagaccg tctggcctgc cgcgacaggc gtttccaagc ctctctcgaa
     3241 ccggatgtct tcttcgtcga cgaacacatt gaggaagcgc cgtagctgac cgtgttcgtc
     3301 gaacaggcgc gcttggagat cggggtgaat ctcgatcgcc gcggtgagcg cctcacgaac
     3361 ggtggcgccc tcgacctcta ccaccgtcgt ggcgtcggtg gcgctccgca gcaccgtggg
     3421 gatcttcagg cggatcatgc ctgcaccacg agtggtatct gggcctgtcg gacgcggctt
     3481 cgaacgactc gatcgtggga cggacctcca gcgccggaca tcgtcgcctc gagcgcatcc
     3541 acggtcttca ggccgttgcc cgtcacgtag gcgaccgttc gctctcgctc gctgatcacg
     3601 ccgcgctcgc gcagccttcg cagcaccgcg atggtcacgc cgcccgccgt ctcggcgagg
     3661 atgccctcgg tccgggccag cagcgtgatc ccctcacgga tctcctcgtc ggacacgtcc
     3721 tcgaccgcgc ccttggtttc gcgcacggca gacagtgcgt acgccgtcgg ccggatcgcc
     3781 gatggccagg gaccgggcga tggttgccgg ccgctgcggg tggatctcgt ccgagccctc
     3841 gcggaacgcc tgcgacacgg gcgagcagcc ggtggcctga gcccccgaga cgcggacaac
     3901 agagggctgc acaaggccgg cttcggccag ctcctcgaag gcctgtcgga tcttggtgag
     3961 gagagcacca gatgcgatcg gcacgacgac atggtccggc agcgtccatc cgagctgctc
     4021 ggcggtctcg aacgcgagcg tcttcgagcc ctcggcgtag taggggcgca ggcccacgtt
     4081 cacgaacgcc cacgggtagg ccccggcgat ctccgagcag agtcgattgg tctggtcgta
     4141 ggagccgtcc accgccacga ccgtcgcgcc ataagcggcg gccgaaacga tcttggccgt
     4201 ttcgaggtct cgtggtacga acacgtagca acgcattccc gccgccgccc cggcgaggtt
     4261 cccagtggag gcgcacgcga ccgtgtcgaa ccccgaacac tcgagccacc gaaagggcca
     4321 cggatgccac atggtccttg aaggagccgg tggggttgac ggtgtcgttc ttgatccata
     4381 ggtcggacag gccgagggca tcgcctaggc gggatgcgcg gcgaagaggc gtccatccgg
     4441 cgccgaggtc cacgcgcggc ggtccggcag gtcgtaaccc tgggcgctga tatcatcgga
     4501 cgaactgagg caagaacgtc cgcgcctccc gaagacgtgc gagcggaggg cgaaccatga
     4561 gcaacaccaa ggactacctc gtccccgtcg tcgtcgagca gaccagccgc ggtgagcgat
     4621 cgttcgacat ctactcgcgg ctactcaagg agcggatcgt gttccttggc acgccgatcg
     4681 acgacaacgt cggaaacctg atcatggccc agctcctgca cctggagagc gaggaccccg
     4741 acaaggacat cagcctttac atcaactcgc ccggcgggga catcacgtcg ctgttcgcgg
     4801 tctacgacac gatgcagtac gtcaagcccg acgtgtccac gatcgtcatg ggacaggcgg
     4861 cctcggctgc ggcggtgctc gtgctggccg gcaccaaggg caagcgctac gcgctgcccc
     4921 actcccgcat cctgctccac cagccgcacg gtggtgcgga ggggcaggcc gtcgacatcg
     4981 agatccaggc cagggagatc acgcggtacc gcaagctcct cgaggagctg atcgcctttc
     5041 acaccggcca acccctggag cgggtgtcca aggacaccga ccgcgacttc atccttaccg
     5101 ccgagggcgc cgtggagtat ggggtcgtgg acgaggtcat caccacccgg aagatcaccc
     5161 ccgaacttgc agcggtgggg ggcgggggct cctagaggat cgagtgaggt cgtggagtgg
     5221 atccgcgaag ggaaggtgcg gagctgagcc ccgtgatcgc gctcactgtg cgggttcgtt
     5281 cccgttagga ggccgtcgct cgccggtggg agatccgctc cagcacaaga aggccggccc
     5341 aagaacaatc acaatccacg gcgccgtgct ccaccagtgc gatggcgtct cggtgattcc
     5401 tcttgcgagg tcccggacgt gtatccggcg ccgaacacgc ctaccactac gagggacagg
     5461 ccgccgatca acaacacccg cgtgcgcatt cgggccccct tccgttcgac atccacgata
     5521 tgaccttgcg tagggaaggg ccagtggtgt cgcgtcgcga ttccgtcgca atctcgctgt
     5581 cgtgcgagcg aaggaagcct cggcccgcct gaacgagcct ctcgcggccg atcgggcgca
     5641 tcggacgtcg acccggtcct cgagccgtcg agacctccga ggacgccgag cggctgcgat
     5701 catcggctcg cagcttcgcc ccctccgctc ccacgcgtcg gcctacgacc gttgggcggt
     5761 ctggatcgcg cggagtgctc gccagttgac cccctcacga aggaaggaaa gactcgagcg
     5821 ccgaaactgg cgccctcgat cgcgcgcaac cggggagact cgaacctaat ctcctgatcc
     5881 gtagtccgga ggccttgctc ctatttcggg ctactgcgtg tagcgacggc aagtgcggac
     5941 gagccgcgaa gctggagggg tcggtttcgg gttggccatc gcaggaggat cgagtcaagt
     6001 gccggtcatc tgctccggtg gcggcattcc cggcggaact tctccacgct gcgtgtactc
     6061 gaggttgctc aattcgaccg ggttgacctg cacggaggcg ccgcgccgac gcatcaggag
     6121 caaggccacc tgagccacga cttccaagag gcggtacagg atgatcgtgg cggccacgct
     6181 gatgaccgag gccggtagca ccaggaggct gtaggcgacg atcaacccga accgatatcg
     6241 tcggccccag atcacagaca gaaggacggg cgacacccag atgagggcga ggacagccgc
     6301 cgaggaaagg gcggagaccg gccgccagcc gaggacggcc aagagcgaat cgagtccagc
     6361 taggccgtag agcgcgaccc aagcggcgat gaggaccttc cacctccagg cccgcaggag
     6421 atgggtggct aggctgatcg cggcccacat gacccagcgc atcaccagcg gaacccgggt
     6481 tccccccatg gcgtcccgga aggtcgcatc ggccttctct cggtcattcg gatccatgcc
     6541 gcccacgacg agctggtcat gcagcaaggc cggcaatgtg tggcggccat accggggaac
     6601 gagccacgtg aggaagctcg gtaccgaagc cagatctgtc gcgtcgcctc ttctgacaac
     6661 gatgtcctga tgccctggtt cctcaaaccg gaatggttca tcgagctcga atcccgtcgc
     6721 tgacacctgc cgaagcagga tctcggcatc gagttcggac caccgcttct tgaagggcag
     6781 gtggagttgg atggacgaac ttcccaccag cgatcggtgc aagctgtcaa ctgcatcgtg
     6841 aaccagccgc gggagcgggt ggccgcttcg caccgccaag tcctggagct gactcagagc
     6901 ctgctcgtcg acctccaccg tgtacgtcac gcaccaattc tgcgtacgcc accacaaccg
     6961 gtcaagagac agtgtgccct cccccaggcg gagcatcact ggttcgctaa gagagctgat
     7021 gaccggtccg ggatcttgga gcgggatcgc cttcaggcgg cttgaggact cccgccttac
     7081 cgaaacccac ccgtgacacg catgccctcg gatcaatctg attcacgggc tgccgatgcg
     7141 acgaagtgag aggagaagat caagatcgtc acgaagtcga tcgccaagtc gtcgtcattc
     7201 gaatggttga gacctatcct gtacaggctt cctcctgtct ttgacagccc tgacgcgcgc
     7261 attggcgtcg gaggttcggt ggacaaggag gccgctatga acgcacggcg ggcagtgttc
     7321 gcgctcttca gagccatccc gctggtcttg gtcgttctct ccacacccgc ggcggctacg
     7381 ggcccaccga ccactgcgac tggctcgttc acctaactga gtttcgatct gtcgaacgaa
     7441 cgcatcgcgg gagtcttcct gttcgatttc actgagaccg acagcttctc cggcacaagt
     7501 gtcctcgagg gcagctgcgt cgtgactcgg cttggtaagc acctgtcaag ggattgagac
     7561 tgcactcgcc tctccatcac gactccatca gggctgacgg gttgtggatg ttcagagact
     7621 ttggaatctt gtggatgttc agaggcttcg gcgaggtgcc gaaggaagat cgcccacgtc
     7681 cgcgggtctc gcgatgggca gagctgaccc gtcgtcacat ctttcgtctt cgcgtgtcac
     7741 ccattcccga gccgggacca acccgatcgg cgatatcact gtgtcgtgcg cgacgcgtcg
     7801 ggcagtgagt tccagatcgc tgacgatccg ccggcgcgga gccgctcaac cccgctccga
     7861 gagcacgagc tcgagccatg cctcggcttc ccgatacagc gtcggattgt tctccggcgt
     7921 gtagtaggcc agggctgcga cagcctgcga gagggcccac ccgcgcgcac gctcccacgt
     7981 tgcgtcgtcg gtcggcagcg cctcgcggaa ggcgtcgcgg gcctcgcccg aatgcagctt
     8041 ccacgcgacc atcacgtcgc aggccgggtc gccgacgccc atggcctccc agtcgatgac
     8101 ggcgcagatg cgcccgtccc gcacgagcca gttccgaacg tcgaggtcgc cgtggtgcca
     8161 gacgggcggg ccctgccacg ccggcgccgc gagcgcgcgc tcccactccc ccaccaccgc
     8221 gggatcgccg tcgaacctgg ctagccagta gcggatctcc tcgtcgcgct cggccagggg
     8281 gatgccgcgg cccatcggcg cgcccgaggg atccaccccc tgcagggcgg cgacgaacag
     8341 agcaaggtcg cgagcggcaa aggtcgggtc gatggcctcg atcggggtcg gcgcgccatc
     8401 cacccatgtg aagacgtccc agaaccacgg gtactcgtcg gtcggacggc cctgggcgac
     8461 cggaacgggg atctccagtg ggaccagcgg cgccagcctc ggcagccagt cgaactcctt
     8521 gccgccggac cgcgtcggtc ccttccggcg tgcgagccgc accgccaact cctcgccgag
     8581 gcggaagatc gcgttgtccg tccctgcggg ctcgacccgg cgaagcggca ggctgaccca
     8641 ctcgggaaac tcatcggcca gcagccggcg cacgagtggc tcatcgatct cgagctcatc
     8701 gccgtgcatc ctcggcatcc gtccgacccc gggtcgatcg cgactcacgt cccccagtcg
     8761 gccgcatgat acggctcgat cggggcgggt cctcgcgcga ctgatggttt cgacctcaga
     8821 tgagttcata cctctttcgg cacaacacgc ggtcctactt tctgaatgtc gcagccagga
     8881 gtcgctgcca cgtctgccca tactgcagcc catgtgctga tgtgccgtga acaccaacgt
     8941 cgcgcatgga ggagcggtgc ttgggcacca gctgaaggtc ctgcgccgcc acgtccggcg
     9001 gccgaggttc cgccccttcg atcgcgtctt cctcgccgcc gcggctcacg tccttccgcg
     9061 ggacacgtgg gcttccttcc tcgtcacgct ccagacgctg ctccggtggc accgcgaact
     9121 cgtccggcgg aagtggacct accgcaccgc gagcaagccg gacaacgaga gcgggcgccc
     9181 gcaccgcccg ctctcgctat cgctatcgcc ttcggctttc agcctcgggc gcctctcctg
     9241 ttaggacggg aaggtccagc tgaggtctgc agtgagcgtc cctcccacga gcgagagcgt
     9301 cccggttgcc gagaacgact cgatcctaac cacctgattc cccggacacg tcagcgtcga
     9361 tgtgaccgtg gctaccgtgc cattgaaggt cgtgttgccg tggttgacgg cggtatcgaa
     9421 ggtcgcgccg atgtcaactg tctcctgctt gttgtccgcc tgcggcttgt tgccaccacg
     9481 gttgtagcag cgcgagaaga gcgtcccgct gccgtcgagg ctccacgtcg catacgcggt
     9541 ggcgttcccg agccccgccg ccttgaacga caggttcagg tcgacctcgt tcccgctcac
     9601 ggtgacggac gttgtcgggc cctggacgaa atgcgggctg tccgccagcg cctgctgagc
     9661 caacaggccc aaaacggcga gtgtcgttac tgttaccaca gcaaatcgtt tcatgggtca
     9721 tctccttgtt ctcgacattt cccgacggat cggatccttt tgtccaatca ggggagcgga
     9781 tccgggagcc aattcagcga atatgcctcc tttctgtgcg cctgccgcct acttgacggc
     9841 gacgcacgga tacggactag tgaggagcaa cctcaggctc actggccgtc tacgaaacca
     9901 catcgatgca gtccgccccc ccttcagccc tagggaatcc gacctaggcc taatggtcca
     9961 ctcaacagcc agttgtggaa taccgcatcc ccaatttggg ggggacggtg ggagttttcc
    10021 gggtgggaat ccgactgacg tgtatgcagt tgatgtgcag ctgacgatgc tcgtcgacac
    10081 cgaggctgaa gagcgcaaaa ccattgactc ggctcccgaa cagggccgcc atcggaccct
    10141 aggaccgccg tgggtctccc aggacgtgct acgagtcacg ctgatcgtgt ggcgcgcatc
    10201 acctatttcg cctgagcaat ccacaagagt caggagtcat ggcggcccat cttccccgga
    10261 gagtgctcac ggcccccgcc accacggggg tgtcgcacca gctcatgagc acggcgggtg
    10321 ccctcgggga tctggccacc gcgctctcaa tcgaatggga cggcattgac gacgacgctc
    10381 gcatcgccct cgtgcggaag attcagcagc tcgcgctgca cttggggcac ctcgcagagc
    10441 gcagcgggcg accaagcttg gagcccaccg accgccagtc aggttctccc tggttcgacg
    10501 gcctttcctt cctcacccca cgcgagcacg agatcctgcg ggccttggcc ctgggagcgt
    10561 ccacggggag gatcggggag cggttcggca tccgcccagc aacggtgagg agccatgtga
    10621 aaagcctctt ggtcaagctc ggggtccact cccggatcga ggcggtctcg gtgtttctga
    10681 aatgggattc ccgccaggag ccccctgtct gatgggaagc ctgccgccgc cagcccctcg
    10741 gtacgaaaac tggatcatgg cgccctcatg tactcgtgga cgagcccgcc gaggagatct
    10801 cgccgttcga tgtcggggca cgccctcggc ctcttcgagg gggcgaggat ctcgagcact
    10861 tgcagctgca atggattccg cgccgccatc cgcagatgag ctatgggtcc ggtatggccg
    10921 ggcaatgttg agaaaacagg tgtctggtcc attggacagc ctggattcct cgtcgtggcc
    10981 gccgaggccg agcaagtcga gcgaacttga agaaagacct ggtcacggga gtgatccgag
    11041 ttcttggccc tacgggattg tcggactccc taggctggcc gcgggggcga atcggggttt
    11101 cgcgccccac aggccatttc agttccgcta gcaacccggg cattgggctc gtacccgccc
    11161 ctgccccgtc gcaggtcagg ttaaggtcat cgcaccgatg cgaaggcccg aacggggaag
    11221 gtgcccatcc cttcaacccg ctgcggaacg gcaaagagat cgaacccctc cgcaggaagc
    11281 gtttcgaggc ccgtgaggtg ctcgatgatc gggatctcgg ccgcaagcag gatcgtgtgc
    11341 acgggccggt ctcccgtatg cgtcccgtcg atgttcaccg aatcgatccc gaccagcgtt
    11401 gctccacccg cgaccagggc ctccgctgcc tcggcggtca ggtaggggtg gccctgctca
    11461 ccgtagcgat cggtccccca gtgacgtgac cagccggtcc gcacgagaac tgcccgctgt
    11521 gcgacctcga gtcctttgag ggcgtccggc ccaatctcgg gacccgtgtc gtcgacgacg
    11581 atcccgggga ccgccgcaac catctccagg ggaaggtcgt cgattcccca cccatcacgg
    11641 taacgatgcg ccggggtgtc gagatacgtt cccgtgtttg ccaccatcga gatctgggcg
    11701 atgcggatct ccgtcccggg cgcgtaacgc tccctggagg cctcccagct caggtgctct
    11761 ccgatctcgg gtcccgggag tccggggtac gtcaccatcc catggcggat cacgtgactg
    11821 agatcaacca accgcatgca ccgctcgcct ccttggccaa gtccccgcga ccttctcgtt
    11881 tggtccggcg atgaaaaccg tccacgaaca tcgcgatcga tccccacccc actgtggctc
    11941 ttgggccgct cctctgttcg accctgtgac cgttgaggag tcgatgagcg ctcccggttc
    12001 cttcgggagg ccgatcctta ttccggcggc caacctcgct catccctccc acgtgaacct
    12061 gtgggggtca aaaactccga tcacccttgt gacctgcggc ttcgcccgat ctggccgccg
    12121 gtcagatcga cgcgaatcgc cttccctaaa gcccgagtga gaaccccgcc gccaaccgag
    12181 ttggcctgag atctgagtcc acgggactca agtcctgcac ggagcggctc cgcactcggg
    12241 cagttcggca gccaaggtag gcgaatcgaa tgggcggaca ctcgcttccc gagcttcctt
    12301 agagtcgctg gctcgtcttc gccgcccgct gcgaatccgc aggtcgagcg cttgatcgga
    12361 gtttctgacc cctacacccg agcctctttg aaccgttccg ccgctcgccc gcg
//
LOCUS       DASUYD010000162        12548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_533596, whole genome shotgun sequence.
ACCESSION   DASUYD010000162 DASUYD010000000
VERSION     DASUYD010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12548
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533596"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VF195_09370"
     CDS             <1..366
                     /locus_tag="VF195_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581066.1"
                     /translation="DGGEEDFTAADFVEQAIGLLQGQPDMEDLIADRIGDALEDDEVE
                     GVDLALVAQAQEAFDEGMAGDTLELLGRSIGEEVGPVLHQPEVGGGLEAPEGTAGPVL
                     IAVAAVLILSGAFIAAKVR"
     gene            368..1432
                     /locus_tag="VF195_09375"
     CDS             368..1432
                     /locus_tag="VF195_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX6581067.1"
                     /translation="MSTEAQPPGAVRPGWGRRFFDALDERLGLRGLAYPVPEHANKLA
                     YSLGGLSLIAFLLLLVTGIALAQFYDPEPATAHESVRRIITEVTLGGFLRGFHYWMAM
                     AMVVLVGLHLLRVFVSGSFKRPREGNWIVGVVLAGITATFFFSGTVLKWDQESIEALE
                     HNIEIGRLLGRFGYWLSPELGGIPLLHRLYTLHIAILPAVFFLVIAVHLLLVKRHGMA
                     PSPFRTGPVPEPTEHFSRHLARLGGFALILIGIVTVLAVIAPPVHGPAPVEGIEVTKP
                     PWPMLWLYPVENWVGVSGILWATLVIFAALLIVPFVDRGEERDPSKRLWVVIPAGIVV
                     LAVLGLIVYALVTPVTTHIG"
     gene            1456..1659
                     /locus_tag="VF195_09380"
     CDS             1456..1659
                     /locus_tag="VF195_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581068.1"
                     /translation="MIFWTAVAVAVFLLGGGVATLRRVVAGGTVEDYIVLSLALVGLA
                     VALLVAGRIALASARAQRRARRS"
     gene            2011..3117
                     /locus_tag="VF195_09385"
     CDS             2011..3117
                     /locus_tag="VF195_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581069.1"
                     /translation="MARTVPVGRRNLLSDRRRVAVSLLGVGLAVALMLLIQGLWSGTL
                     DRITAYEDNVGAQLFVAEPGTRSFQSDVSAIPPSVEDQIREVPGVQAADGVAARQLIL
                     ELHGTKMPLTLIGTRTGGLGGPWALEAGRAVTADDEIVIDAGLAADHGYRLGEQFELL
                     GVRLEIVGLTPDSRALGNGGYVFVSLATAGLVLGAPDATSFVLVRTSDPVAVASEIES
                     RTGREALTAEEIARGDRALYDDTMGSVIRVMLLIAFAAGTLIAALSVYSSVIDRIREY
                     GIVKALGASRRRLLGIVLAQTGALALAGGVAGLGLFLAAKVAVGAWLPQFHIELPASI
                     LVAAFVVVAAMALVAAILPARRLGRLDPASVYRG"
     gene            3123..4226
                     /locus_tag="VF195_09390"
     CDS             3123..4226
                     /locus_tag="VF195_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6581070.1"
                     /translation="MGARVAVGRRNLFSERRRGLLGVAGTAAALLLVLAVGAVFDGAM
                     RQITRYIDTQPAQVFVSQLGVRNLHMASSSVPLSAIEETGSVTGVTSVAPILFAQDGL
                     ETDDGRRLSYVIGYEPGRAGGPAEIETGDGPGPGEIVLDTATAASLGVDIGDQVGALG
                     TSWRVSGLVSNLSNVANGAAFVRLDDFAVARGLPDTASYLLVTTDRDPASVAAAIERA
                     SGLSATTREAFSRSERELVSDMIGDVLRIVTGAAWLVGFAVVALTLYAATRSRLREIG
                     VMKALGAGRGALSRVVLAQAAWSVGAAVVLAAALTFGLGSLLARTSSTVTLAIEPGAL
                     VRVAAISVIVGAAASVAPLIKVSRVDPASVFRR"
     gene            4229..5065
                     /locus_tag="VF195_09395"
     CDS             4229..5065
                     /locus_tag="VF195_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6581071.1"
                     /translation="MQALMRAEGLERVFGEGDVQVRAVRGVDLEVRPGEVVLIMGPSG
                     SGKTTLLSMLGAMLRPSAGRVEVDGVDLASLAEKRLPAFRARRFGFIFQDFNLLSALT
                     VIENVELALNLAGVTGRRARERAEELLRGLGLGERLEFLPDKLSGGEKQRVAIARALA
                     NEPAVILADEPTANLDSKIGHEIARLLRRIAGEEGRSVVIVSHDTRLKDIADRVLWLE
                     DGEFRSMSEMATDPVCGMAVEREATPHFVHDGQTWFFCSTACRDEFASAPDRFSAGAR
                     VA"
     gene            5080..5346
                     /locus_tag="VF195_09400"
     CDS             5080..5346
                     /locus_tag="VF195_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6581072.1"
                     /translation="MEPRHEIVTRLRRVKGQVAGVGRMYEEGRYCIDVLDQLAAARAG
                     LESAALLVLENHVAGCVPDAMEGEGGTEKARELDSAVRRFVMSV"
     gene            5365..5814
                     /locus_tag="VF195_09405"
     CDS             5365..5814
                     /locus_tag="VF195_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581073.1"
                     /translation="MKRYEVYSRFARRHLDTRQERAVYLTLVGQEADSWSARELAERT
                     HLDPGETERVLEAFRADGVADAIDAPKGRRYRWRSDMNYLFGEHGGSPEWVDPVCGMP
                     VLAKSPYVAYDTYRRPRKFCSSLCLATFLASPSAFSGPASVEARGAV"
     gene            complement(6285..7457)
                     /locus_tag="VF195_09410"
     CDS             complement(6285..7457)
                     /locus_tag="VF195_09410"
                     /inference="COORDINATES: protein motif:HMM:NF019262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome domain-containing protein"
                     /protein_id="HEX6581074.1"
                     /translation="MKPSVRIAATGAIAILALVVLPATAWGHGGMEEEPGQEQGVMES
                     GHGAEEGAGEEAEVPLIELGVPEGAELGSRVTLRATLTDPHDGRPIADASIVFQAPAV
                     WGEEIRGWMVLGAATTDRKGTASLTTQVRTSGEVEIRAVFLGGEGFAPASGSAHLDVL
                     GDAQLYEPAVGIRVPWLNLWLLAAVIGLVWGMYLLVGRRVLAIARPERAVVAVGEGST
                     PPSSGATSRRSFLARALPFGAQAGIAVMGTGLVALVARSPRTHGNLMAPPATLDYHRT
                     AVARVGESMEMQPMPPALDRPVSFSREVLPIFLASGGPHVVAPEHSPPPGGLRLDSYE
                     GVMGKEGVVVPGRPEESELVEHLLSVGMQMPPSVPPLPGEQIQLIVTWIAQGASDN"
     gene            complement(7454..8854)
                     /gene="nrfD"
                     /locus_tag="VF195_09415"
     CDS             complement(7454..8854)
                     /gene="nrfD"
                     /locus_tag="VF195_09415"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6581075.1"
                     /translation="MADVVDRSTREGRSPELLEETALAPLRGTSPGYYLLIIALLALV
                     GLGAYAYSIQLRQGLAVTGMAATANKVMWGLYITNFVFFIGISHAGTLISAILRISNA
                     GWRTPITRMAEFITVVALSVGALFPIFDLGRPDRLLNVIQYGRWPSPILWDFFAISTY
                     LVGSLLYLYLPLIPDLATCRDRLAGSLSRRKAWLYRTLSLGWTGSREQRRNLAAALGT
                     MMIVIIFVAVSVHTVVSWIFAMTLRTGWNTAVFGIYFVAGAIFSGIATLIIVMAILRR
                     VYHLERYVTERQFVNLGYLLAALAMIMLYFNVSELVTTGYKLEGSSPVYLGQIFTGSF
                     APMYWFYIFAGLLLPGLVMLFRRTRTITAVVIIAILVDVSMWLERFIIVVASLRVPQM
                     PYPSPVMYFPTWIELAISIGSLALFALLIAIFVKIFPVLSIWEIREHEGIVSTPMVVP
                     VPARIGSPSGPEVVGP"
     gene            complement(8847..9827)
                     /locus_tag="VF195_09420"
     CDS             complement(8847..9827)
                     /locus_tag="VF195_09420"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6581076.1"
                     /translation="MPISRRDLLKGAGAVGATLALLPDPVLAHGGEGEEMGGEEHRGD
                     ESESQRVRRWMMVIDLRACDGCAGLGVPPQCTQGCNWARFVPEGQQWIEVYELEEGHL
                     EGIGSHFMPAPCMQCQNAPCVNVCPVGATFHSPEGTVLIDQERCIGCRLCMAACPYDR
                     RFFNWGEPVQPPQVRSAPYDILTQVPAIEGTVMKCDLCTDRMAAGGLPTCVEMCPRGA
                     IWVGDLEEGIATNGRRMASVESLMSENGVGRYKEELGTEPRVYYIPGHGQDAATEGEE
                     AEAFGEWQTPGVGLMKDRLEWPWRKINEELAKLETVPAATGTAMEAMGDG"
     gene            complement(9889..10545)
                     /locus_tag="VF195_09425"
     CDS             complement(9889..10545)
                     /locus_tag="VF195_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581077.1"
                     /translation="MTVSLLIVDDHEVVRVGLATLLGRRDDLRLAGVAASGAEGVALA
                     SKTRPDVVVMDVRMPGESGIEACREIRSCLPGTRVLMLTSYADEDAVLASILAGASGY
                     LLKDTTGQDLAEAILRVAAGASLLDPAVTGLVLSRLKADSGLAEGALTDRERQILELI
                     ALGKTNREIGEVLYLAEGTVRNLVSGILAKLGVGTRAEAAARFARHVPDGGSARPRNS
                     "
     gene            complement(10542..11738)
                     /locus_tag="VF195_09430"
     CDS             complement(10542..11738)
                     /locus_tag="VF195_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX6581078.1"
                     /translation="MAREAYMHLRRIKVLSILVPLAGLLGFEVVRHWLIQPAMGETAQ
                     HLDEHLISGVVLFVAVVAFSFAIFRLLERLHDQLVALNEAAIAVTGDLSVDYVLERVA
                     ELARTVADASYASVDVRAGRERTVDSGTRPGEGESLALPIVVRGARLGELVLAGPRDG
                     RFRNSDRRALETFATQAGIALENARLFEQEQELAATRERVRIGMDLHDGVIQELYGLR
                     LKAEDAAQLATADASEAAALLQDIHSSLGHVIGEVRTYIYGLRDGDRSVELHPALESL
                     VAEFSTARTELRLGVRGDARLPAATVAHLLHVAREAVANSLRHSGASRVIVDVEASGD
                     VVRLTVSDDGSGFEPDAPSMGLGMRDMRDRARWCGSELLVESAPGCGTTVSIAVPADG
                     QKAVLQ"
     gene            complement(11918..>12548)
                     /locus_tag="VF195_09435"
     CDS             complement(11918..>12548)
                     /locus_tag="VF195_09435"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX6581079.1"
                     /translation="DTIAVWNGGMSLLGGLIGAVLVGGFIARREKLTVLPLLDMAAPG
                     IALGIAIGRVSDLIIGDHLGKATTLPWGFRYVGGVEPLAGVPPVGTVVHPVALYDLVL
                     TIVLLVVLIRFIRSPRATGSAIALFTLWYASGRILTDFLRTDPRRLLGMTGSQLTAIA
                     AVVAVSLALWWRARRGVGARVEDPPGGAAPPHQVRWKGQGVPEAEAAPR"
BASE COUNT         1947 a         4328 c         4306 g         1967 t
ORIGIN      
        1 gacggcggcg aggaggactt cacggcggcg gacttcgtgg agcaagccat cggactcctg
       61 cagggccagc cggacatgga ggatctgatc gccgacagga tcggcgacgc cctggaggac
      121 gacgaggtcg agggggtcga tctggcgctg gtggcgcagg cccaggaggc cttcgatgag
      181 ggcatggcgg gtgacacgct cgaactgctc gggcgatcga tcggcgagga ggtcggcccg
      241 gtgctgcacc agcccgaggt gggagggggc ctcgaggccc ccgaggggac cgcgggtccg
      301 gtcctgatcg cggtcgccgc cgtgctgatc ctgtccggag cgttcatcgc ggcgaaggtg
      361 cgctgatatg agcacagaag ctcagcctcc gggtgccgtt cgtcccggct ggggacggcg
      421 cttcttcgac gccctggacg agcgcctcgg cctgcgaggc ctcgcctacc cggtgcctga
      481 gcacgccaac aagctcgcct acagcctcgg agggctctcg ctcatcgcgt tcctgttact
      541 gctcgtaacc ggtatcgcgc tcgctcagtt ctacgatccg gagcccgcca cagcgcacga
      601 gagcgtccgc cggatcatca ccgaggtgac gctcggcggc ttcctacggg gctttcatta
      661 ctggatggcc atggccatgg tggttctcgt cgggctgcac cttcttcggg tcttcgtgtc
      721 cggatcgttc aagcgccccc gcgagggcaa ctggatcgtc ggcgtggtgc tcgcggggat
      781 caccgcgacc ttcttcttct ccggcaccgt gctgaagtgg gaccaggagt ccatcgaggc
      841 cctggaacac aacatcgaga tcgggcggct gctcggccgt ttcggctact ggctctcgcc
      901 cgagctcggc ggcatcccgc tgcttcaccg cctctacacc ctgcacatcg ccatcttgcc
      961 cgccgtgttc ttcctagtga tcgccgtcca cctgctgctg gtgaagcggc acggcatggc
     1021 gccctcgccg ttccgcaccg ggcccgtgcc cgagcccacc gaacacttct cgcggcacct
     1081 cgcgaggctc ggcgggttcg ccctgatcct aatcgggatc gtgaccgtgc tggcggtgat
     1141 cgcaccaccg gtgcacgggc cagccccggt cgagggcatc gaggtcacga aacccccttg
     1201 gccgatgctg tggctctatc ccgtcgagaa ctgggtcggc gtgtcgggga tcctgtgggc
     1261 gaccctcgtg atcttcgctg ccctgttgat cgtgcccttc gtcgaccgcg gcgaggagcg
     1321 cgatccctcc aagcgcctgt gggtggtgat ccccgcaggg atcgtcgtcc tcgcggtgct
     1381 cggtctgatc gtctacgcgc tggtcacgcc tgtgaccacg cacatcgggt gaccgtgatg
     1441 cccaggcgca ccgtaatgat cttctggacc gcggtcgcgg tggccgtttt cttgctcggc
     1501 ggaggggtcg ccacgctgcg gcgcgtcgtg gccggcggca cggtcgagga ctacatcgtg
     1561 ctgtcgctcg ccttggtcgg gttggcggtc gccctcctcg tggcaggtcg gatcgcgctc
     1621 gcatcggccc gggctcagcg tcgggctcgc cggagctgag gcggaacttc gaacgctcac
     1681 cgaatcttca agcagccttc accccgcctg catctcgaag tcctacggtt ctcatggact
     1741 acccccccag ggtatccatg gaacgcggaa ccaggaggtg caacgtgaac gcgagcgatg
     1801 ccggagtcgg cggccgactc acgcacccgg gacgggaacg aggactcctt gcgcatgggt
     1861 cttcggccac ttcggcaaga gcgcctcgct cccccagggc gacctcgtcg ccggtggagt
     1921 tcctaccgac ggaggccgga tccacgagtt cacgtgccgg ttgtgatgtt ccgcggtcga
     1981 tcggccgtgg ggcgtgagga ggtaacaggg atggcccgga ccgttcccgt gggacggagg
     2041 aacctgctct cggaccgtcg gcgggtggcc gtgagcctgc tcggcgtcgg gctcgccgtc
     2101 gcgctcatgc tgctgatcca gggcctatgg agcgggacgc tggaccggat cactgcctac
     2161 gaggacaacg tcggtgcaca gttgttcgtg gcagagcccg gaacgcggag cttccaatcc
     2221 gacgtctcgg ccatcccgcc ctcggtggaa gaccagatcc gcgaggtccc cggcgtccaa
     2281 gccgcggacg gggtcgcggc gcggcagctg atcctcgagc ttcacggtac gaagatgccg
     2341 ctcacgctga tcggcacccg gaccggaggg ctgggcggcc cgtgggccct cgaggccggt
     2401 cgcgcggtaa ctgccgacga cgagatcgtg atcgacgccg gcctcgcagc cgaccacggc
     2461 taccgcctgg gcgagcagtt cgagctgctc ggcgtccggc tggagatcgt gggcctgacg
     2521 cccgactccc gggccctcgg gaacggtgga tacgtgttcg tctcgctggc gacggccggc
     2581 cttgttctcg gggcgccgga cgcgacgagc ttcgtgctcg tgcggacgtc ggaccctgtc
     2641 gccgtcgcga gcgagatcga gtcgcggacc ggccgggagg cgctcacggc cgaggagatc
     2701 gcgcgcgggg accgagccct gtacgacgac acgatggggt cggtgatccg cgtgatgctg
     2761 ctgatcgcat tcgccgccgg cacgttgatc gcggcactat ccgtctactc ctcggtcatc
     2821 gaccggatcc gcgagtacgg gatcgtgaag gcgctcgggg cgagccggcg ccggctgctc
     2881 gggatcgtgc tcgcgcagac cggggcgctc gctctcgccg gcggcgtcgc cgggctcggc
     2941 ctgttcctgg ccgcgaaggt ggcggtcggc gcctggctcc cccagttcca catcgagctg
     3001 ccggcctcga tcctggtggc ggccttcgtg gtggtggccg cgatggcgct cgtcgcggcg
     3061 atcctgccgg cccggcgcct cgggcgcctg gatccggcgt cggtgtatcg ggggtgagga
     3121 cggtgggtgc tcgcgttgcg gtagggcgta ggaacctctt ctcggagcgg cggcgcggcc
     3181 tgctcggggt ggccggcacc gccgccgccc tgctgctcgt gctcgccgtc ggggccgtct
     3241 tcgatggagc gatgcggcag atcacccgct acatcgacac ccagcccgcg caggtgttcg
     3301 tgtcccagct cggcgtgcgg aacctgcaca tggcctcgag ctccgttcct ctctctgcga
     3361 tcgaggagac cgggtcggtg acgggtgtga cgtcggtcgc cccgatcctg ttcgcgcagg
     3421 acgggctcga gacggacgac ggtcgccggc tctcgtacgt gatcggctac gagccggggc
     3481 gcgcgggcgg gcccgccgag atcgagaccg gtgacgggcc cggtccgggc gagatcgtcc
     3541 tggacaccgc gaccgccgca tcgctgggtg tggacatcgg cgaccaggtg ggcgcgctcg
     3601 ggacgtcgtg gcgcgtctcg ggtctcgtga gcaacctgtc gaacgtcgcc aacggggccg
     3661 ccttcgtccg actggacgac ttcgccgtcg ctcgcggcct ccccgacacg gccagttacc
     3721 tgctcgtgac aaccgatcgt gaccccgcga gcgtcgccgc tgcgatcgag cgagcgtccg
     3781 gtctgtcggc gaccacgcgc gaggcgttct cgcgatcgga gcgcgagctc gtgtccgaca
     3841 tgatcggcga cgtcctgcgg atcgtgaccg gcgcggcctg gctcgtcggc tttgccgtcg
     3901 tcgcgctcac gctgtatgcg gccacgaggt cgcggctccg tgagatcggc gtgatgaagg
     3961 cgctgggcgc cggcaggggt gccctgtcgc gcgtcgtcct cgcgcaggcg gcgtggagcg
     4021 tcggcgccgc ggtcgtcctc gcagcggcac tgacgttcgg gctcgggtcg ttgctcgcgc
     4081 ggacgtcgtc gaccgtcacg ttggcgatcg agcccggcgc gctggtccgc gtcgcggcga
     4141 tctcggtgat cgtcggcgct gcggcctcgg tcgcgccgct gatcaaggtc tcgcgggtcg
     4201 accccgcgtc cgtcttcagg aggtaaccat gcaggcattg atgcgggccg aagggctcga
     4261 gagggtgttc ggcgaaggag acgtgcaggt ccgagccgtg cgaggcgtgg acctcgaggt
     4321 ccggcctggc gaggtcgtcc tcatcatggg tccttcgggg agcggcaaga cgacgctgct
     4381 ctcgatgctg ggtgcgatgt tgcggccgtc ggccggccgt gttgaggtcg acggggtgga
     4441 tctcgcctcc ctggccgaga agaggctccc ggccttccgc gccaggcggt tcgggttcat
     4501 cttccaggac ttcaacctgt tgtccgcact caccgtgatc gagaacgtcg agctggcgct
     4561 gaacctcgcc ggcgtcaccg gaaggcgcgc gcgcgagcgc gccgaggagc tgctgcgggg
     4621 tctcgggctc ggcgagcgac tcgagttcct cccggacaaa ctgtcgggcg gcgagaaaca
     4681 gcgcgtggcg atcgcacggg cgctcgcgaa cgagccggcg gtcatcctcg ccgatgagcc
     4741 gacggcgaat ctggactcga agatcggcca cgagatcgct cggctgctgc gccggatcgc
     4801 gggcgaggag ggtcggagcg tcgtcatcgt gagccacgac acgcgcctga aggacatcgc
     4861 cgaccgggtg ctgtggcttg aggacggaga gttccgctcg atgagcgaga tggcgacgga
     4921 tccggtctgc gggatggcgg tggagcgcga agcgacaccg cacttcgtcc acgacgggca
     4981 gacctggttc ttctgctcga ccgcctgccg cgacgagttc gcttcggccc cggaccggtt
     5041 ctcggccggc gcgagggtgg cctgaggagg ttgaggctgg tggaacccag acacgagatc
     5101 gtgacccgac tccggcgcgt gaaggggcag gtcgccggcg tcggccggat gtacgaggag
     5161 gggaggtatt gcatcgacgt actcgatcag ctcgccgccg cgcgcgccgg gctcgagtcc
     5221 gcggcgctac tggtactcga gaaccacgtc gcgggatgtg tcccggacgc gatggaaggc
     5281 gagggcggta cggagaaagc gcgcgagctc gactcggccg ttcgcaggtt cgtcatgagc
     5341 gtgtgaggag gaaccttgtc cagcgtgaaa cgctacgagg tgtactcgcg gttcgctcgc
     5401 aggcacctgg acacacgtca agagcgggcc gtgtacctga ccctggtcgg tcaggaggcc
     5461 gactcctggt cggcccgcga actcgcagag cgcacccacc tggatccagg tgagactgaa
     5521 cgggtcctgg aggcgttccg ggccgacggt gtggccgacg cgatcgacgc tccaaaaggt
     5581 cgtcgctacc ggtggcgatc ggacatgaac tacctgttcg gcgagcacgg cggctccccc
     5641 gaatgggtcg accccgtgtg cggtatgccc gtgctcgcga agagcccgta cgtggcctat
     5701 gacacgtatc ggaggcccag gaagttctgc tcttcgctct gcctcgcgac gtttctggca
     5761 tcgccaagcg ctttcagcgg gccggcgtct gtcgaggccc ggggggcggt gtagacgagt
     5821 gagtccggct tatgtcgttc gcacattccc ccgacctcgc ggccggccgc gtcctcatca
     5881 aggggtgaca tggtgagcgg gcttggatcc ttgacgccta tcgaacgtgc gaggcgattc
     5941 tggacggcac acggccggtc cgccgacgac ctgactgtcc tgcacgcctt tctgcaacaa
     6001 ccccaccgtg tcttggagcc cggagggttt gtccatgtgg tacgggatcc ggatcgaccg
     6061 ggctcatcgg atcgtcgagg agctcgcccg gtgcagcatc gtgagacccg cacgacccac
     6121 gaagcgtggc taccgatgga acgacgcgtt ggactgggcg gtccccgcac cgcagccacc
     6181 cggcggatcg tgcaggaacg ttgggctgcg aaagctggat gcacggtcag tgctgtctac
     6241 ggtggcatgt ccgacggcgt cgctacgatc tcgggaggac gacgttagtt gtcactcgct
     6301 ccctgggcga tccacgtcac gatcagctgg atctgctccc ccggcagggg cggcacagaa
     6361 gggggcatct gcatccccac ggagaggagg tgctcgacga gctcgctctc ctcgggcctg
     6421 ccgggcacca ccacgccctc cttgcccatg acgccctcgt aggaatcgag gcgcaagcct
     6481 cctggcgggg gcgagtgctc gggggcgacg acgtgcggtc cgccgctggc caggaagatc
     6541 ggaaggactt cccgagagaa gctgaccggc cgatcgagcg ccgggggcat cggttgcatc
     6601 tccatcgact ccccaacccg cgccaccgcg gtgcggtggt agtcgagcgt cgcgggtggg
     6661 gccatcaggt tcccgtgggt ccgcggcgag cgcgccacca gcgccacgag cccggtgccc
     6721 atcacggcga tgcccgcttg cgcgccgaac ggcagggccc tggcgaggaa cgagcgacgc
     6781 gaggtggccc ccgacgacgg tggcgtcgac ccctccccca cggcgacgac cgcacgttcc
     6841 ggtcgggcga tcgccaggac ccgacggcca acgagcaggt acatgcccca cacgaggccg
     6901 atcacggcgg ccaggagcca caggttcagc cacgggaccc ggatgccgac cgccggctcg
     6961 tagagctgcg cgtccccgag gacgtccagg tgggcggatc cggaggcggg cgcgaaacct
     7021 tcaccaccga ggaacaccgc tctgatctcc acctcacccg aggtgcgcac ctgtgtcgtc
     7081 aggctcgcgg tccctttacg gtcggtggtg gctgccccga gcaccatcca accacggatc
     7141 tcttctcccc agaccgcagg ggcctggaag acgatcgacg cgtccgcgat cgggcggccg
     7201 tcgtgaggat cggtgagggt ggcccgaagc gtgacgcggc ttccgagttc tgccccctcc
     7261 gggaccccga gctcgatcag aggaacctcg gcttcttccc ctgccccttc ctccgccccg
     7321 tgcccagact ccatgacgcc ttgctcctgc ccgggctcct cctccatgcc gccgtgcccc
     7381 caggcggtgg ccggaagcac gacgagcgcc aggatcgcga tcgcgccagt ggcggcgatc
     7441 cgcaccgagg gcttcatggc ccaaccacct cgggtcccga ggggctcccg atccgcgcgg
     7501 ggaccgggac gaccatgggt gtggacacga tcccctcgtg ctctcggatc tcccagatcg
     7561 acagcacggg gaagatcttc acgaagatgg cgatgaggag tgcgaagagc gcaagggatc
     7621 cgatggagat cgccagctcg atccaggtcg ggaagtacat cacggggctc gggtacggca
     7681 tctgcgggac ccgaaggctg gccacgacga tgatgaacct ctcgagccac atcgagacgt
     7741 ccacgaggat cgcgatgatc acgactgcgg tgatcgtccg cgtccggcgg aacagcatga
     7801 ccaacccggg aaggagcaac cccgcgaaga tgtagaacca atacatgggc gcgaacgacc
     7861 cggtgaagat ctggcccagg tagacggggg agctcccctc gagcttgtac cctgtggtca
     7921 cgagctcgga gacgttgaag tagagcatga tcatcgccag ggccgcgagc agatagccga
     7981 ggttcacgaa ctgccgctcg gtgacgtagc gctccaggtg gtacacgcga cgcaagatgg
     8041 ccatgacgat gatcagcgtt gcgatcccgg agaagatcgc gcccgcgacg aagtagatcc
     8101 cgaagaccgc cgtgttccac ccggtccgca acgtcatcgc gaagatccac gagacgaccg
     8161 tgtggaccga caccgccacg aagatgatga cgatcatcat cgtcccgagg gcggcggcga
     8221 ggttccgcct ctgctctcgg gatcccgtcc accccaacga cagggtccgg tagagccagg
     8281 ccttgcgacg cgagagcgaa cccgccagcc ggtcccggca cgtcgccaga tccgggatca
     8341 gcggaaggta caggtacagc aggctgccca ccaggtaggt ggagatcgcg aagaagtccc
     8401 acaggatcgg cgagggccac ctaccgtact ggatgacgtt cagcagccga tcgggccgcc
     8461 cgaggtcgaa gatcgggaac aaggcgccga ccgacagggc cacgacggtg atgaactcgg
     8521 ccatacgggt gatcggcgtc cgccagccgg cgttgctgat gcgaaggatc gccgagatca
     8581 gcgtgccggc gtggctgatg ccgatgaaga agacgaagtt ggtgatatag aggccccaca
     8641 tcaccttgtt ggcggtggcg gccatccccg tcacggcgag accctgccgg agctgaatcg
     8701 agtacgcgta cgcgcccagg cccaccagcg caagcagggc gatgatcagc aggtagtagc
     8761 cggggctggt cccgcgaagg ggagccaacg cggtctcctc gaggagctcg ggggaccgcc
     8821 cttcgcgcgt cgatcgatcg acgacgtcag ccatcgccca tcgcctccat cgccgtcccc
     8881 gtcgccgcgg gcacggtctc gagcttggcg agctcctcgt tgatcttccg ccacggccat
     8941 tccagacgat ccttcatcaa gccgactccc ggggtctgcc actcgccgaa cgcctcggcc
     9001 tcctctccct ccgttgcggc atcctggccg tgccccggga tgtagtagac gcgaggctcg
     9061 gtcccgagct cctccttgta ccgcccaaca ccgttctcgc tcatgagact ctccaccgag
     9121 gccatccgtc gcccgttcgt ggcgatcccc tcctcgagat cacccaccca gatcgccccg
     9181 cgcggacaca tctcgacgca cgtcggcaga ccgccggccg ccatgcggtc cgtgcatagg
     9241 tcgcacttca tgacggttcc ctcgatcgcc ggaacctggg tcaggatgtc gtacggagcg
     9301 gatcggacct gcgggggctg cactggttcg ccccagttga agaaccggcg gtcgtaggga
     9361 caggcggcca tgcagagcct gcacccgatg caccgctcct ggtcgatcag gaccgtgccc
     9421 tccggcgagt ggaacgtggc gcccaccgga cagacgttca cgcatggcgc gttctggcac
     9481 tgcatgcacg gcgcaggcat gaagtggctg ccgatgccct cgaggtgccc ctcctcgagc
     9541 tcgtagacct ctatccactg ctggccctct gggacgaaac gggcccagtt gcacccctgg
     9601 gtgcactgcg gtgggacccc caggccggcg cacccgtcgc atgccctgag gtcgatgacc
     9661 atcatccacc ggcggacgcg ctgcgactcg ctctcgtcgc cccggtgttc ctcgcccccc
     9721 atctcctcgc cctcgccgcc gtgcgccagg acgggatccg gaaggagcgc gagggtggcg
     9781 ccgacggcgc cggcaccctt cagcaggtca cgtctggaga tcggcatctc gaggcaagcg
     9841 tgcgtgcgcg cggacggatg cgccagggcc acccctcagg gccgattgtc acgagttcct
     9901 gggacgggcg gatccgccgt caggcacgtg cctggcgaag cgcgcggcgg cctccgcacg
     9961 agtgccgacg ccgagcttgg cgaggatccc actcaccagg ttccggaccg ttccctccgc
    10021 gagatacagg acctctccga tctcacggtt cgtcttgccc agggcgatga gctccaggat
    10081 ctgccgctcc cggtccgtca gggcgccctc ggccagtccg ctgtccgcct tgagccggga
    10141 cagcaccaga cccgtgacgg cggggtcgag gagcgaggcg cccgccgcca cccggaggat
    10201 ggcctccgcc agatcctgcc ccgtcgtgtc cttcagcaga tacccagagg cacccgcaag
    10261 gatcgaggca agcacggcgt cctcgtcggc gtacgaggtc agcatgagca cccgggtgcc
    10321 gggcaaacac gaccggatct cccggcacgc ctcgatgccg ctctcgcctg gcatccgaac
    10381 gtccatcacg acgacgtcgg gtcgcgtctt cgaagcgaga gccacgccct cggctccgct
    10441 cgcggccacg cccgccaacc gcagatcgtc gcgcctgccg agcagcgtcg ccaggcccac
    10501 ccgaacgacc tcgtggtcgt cgacgatcaa cagcgagacc gtcactggag caccgccttc
    10561 tgcccatcag caggcactgc gatgctcacg gtcgtcccac atcccggcgc cgactccacg
    10621 aggagctccg aaccgcacca gcgggcgcgg tcccgcatgt cgcgcatccc gagccccatc
    10681 gagggggcat ccggctcgaa accggacccg tcatcgctca ccgtgagccg cacgacgtcg
    10741 ccgctggcct cgacatcgac gatcactcgg gaggcgcccg aatgtcggag cgagttcgcc
    10801 accgcctccc gagccacgtg gaggaggtgg gccacagttg cggcgggaag cctcgcatcg
    10861 ccgcgcacgc ctagcctcag ttccgtccgc gcggtggaga actccgcgac gaggctctcg
    10921 agcgccggat ggagctccac agaccggtcc ccgtcgcgaa ggccgtagat gtaggtccgg
    10981 acctcgccga tcacatgacc gagcgacgag tggatgtcct gcaacagggc tgcggcctcg
    11041 gacgcgtcgg cggtggcgag ctgagccgcg tcttcagcct ttagccgaag cccgtagagt
    11101 tcctggatca ctccgtcgtg caggtccatc ccgatccgaa ctcgctccct cgtcgcggcc
    11161 agctcctgtt cctgctcgaa caggcgggcg ttctccagcg cgatccccgc ctgggtggcg
    11221 aacgtctcca gggcccttcg atctgagttt cggaaccggc cgtcccgcgg cccggcgagc
    11281 acgagctcac cgaggcgcgc gccccgcacg acgatcggga gggcgaggct ctcgccctct
    11341 cccggacgcg tccccgagtc caccgtccgc tcgcgccctg ctcgcacgtc gacagaggcg
    11401 taggaagcgt ccgctactgt ccgggcgagc tctgccaccc gctcgagcac gtaatcgacg
    11461 gagagatcac cggttacggc gattgcggcc tcattcaggg ccacgagctg atcgtggagc
    11521 cgctcgagga gtcggaagat ggcgaaggag aacgccacga ccgcgacgaa cagcacgacg
    11581 cccgagatca gatgctcgtc gagatgctgc gcggtctcgc ccatcgcggg ctggatcagc
    11641 cagtgccgca ccacctcgaa accgagcaac cccgcaagcg ggaccaggat cgagagaacc
    11701 ttgatgcgtc gaagatgcat atacgcctcg cgagccatga aacggctcgg cgctcgaccc
    11761 gagtagggtc tgtcgccccc tcgggagcgg tgccaaggtc cctgagccca tcgatcgtgg
    11821 gtgcctgccc gcccccggcc gatcccgagg ttcaacgtct cggtccccgc cggcaaccgt
    11881 gacgaagccg atcgccgact cggtgcggcc gcagccccta ccggggcgcc gcttccgcct
    11941 cgggcacgcc ctgtcccttc cagcggacct gatgtggggg agccgcccct ccagggggat
    12001 cctcgacccg cgcgcccacg cccctacggg cgcgccacca cagggcgagt gaaacggcca
    12061 caaccgccgc gatcgccgtc agctgactcc ccgtcatgcc gaggagccta cgcggatcgg
    12121 tcctcaggaa gtcggtgagg atccgaccgc tcgcgtacca cagggtgaac agcgcgatgg
    12181 cggatccggt cgctctgggc gaccggatga atcggatcag aacgacgagc aggacgatgg
    12241 tgaggaccaa gtcgtacagc gccaccgggt gcaccaccgt gccgaccggc ggcacgccgg
    12301 cgagcggctc cacaccgccg acgtaacgga acccccacgg aagcgtcgtc gccttgccca
    12361 gatggtcacc gatgatcagg tcgctcacgc ggccgatggc gatcccgagc gcgatccccg
    12421 gcgccgccat gtccaggagc ggcagcaccg tgagcttctc ccggcgggcg atgaaccccc
    12481 cgaccagcac cgcgccgatc aggccgccca gcaggctcat ccccccgttc cagacggcga
    12541 tcgtgtcc
//
LOCUS       DASUYD010000163         9659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_534732, whole genome shotgun sequence.
ACCESSION   DASUYD010000163 DASUYD010000000
VERSION     DASUYD010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9659
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534732"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="coxB"
                     /locus_tag="VF195_09440"
     CDS             <1..1135
                     /gene="coxB"
                     /locus_tag="VF195_09440"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEX6581080.1"
                     /translation="SSEELRETEGTVRKRLLWATAGIGLLLLLSGCAPDATQDTLQPQ
                     GPYAQKLKDLFIPVFWVAVFVFIVVEGGIVWIAIRYRHRKGRERMPAQIHGNTRLEIG
                     WTIAPALILAGVMVPTVATIWDLARKPPDDAINVTVRGLQWWWDFEYTDDDMRTTYGD
                     QASIRTADVLVIPEDRVIYLSLEGAGGDIGGEDDEAKDYMVIHSFWIPELAGKQDVVP
                     GRTNHILMQADHPGMYEGQCAEFCGLQHGKMLVRVRALSAEDWQAWVENQKRASAEPT
                     GLAGDGMDLFLAPLSDGRGSCVTCHAVGGTEATALAGPDLTHFADPTHECFAGCNWET
                     TDREALEAWLRNPDAVKLGSKMPNYHLREDEIDALVAYLYSLT"
     gene            1168..3063
                     /gene="ctaD"
                     /locus_tag="VF195_09445"
     CDS             1168..3063
                     /gene="ctaD"
                     /locus_tag="VF195_09445"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEX6581081.1"
                     /translation="MATVSSSGRRGGILRRPTALTGFSSWFTTIDHKKIGFLYGATAF
                     VFFIVGGVEALLIRAQLAQPDGTLLNADQYSEVFTMHGITMIFLFVMPMAAAFFNYFI
                     PLMIGARDVAFPRLNAYSYWVFLLGGLFLYSSFVLGGAPNGGWFGYAPNSTTDPSIGM
                     SFYAIGLIITGIASTAGAVNLAVTVINMRAPGMSLFRVPVFVWMGLVVQLLLAFSLPI
                     ITVALFQLLFDRELGTHFFDPMAGGDPILWQHLFWLFGHPEVYVLILPAFGIFSEVLP
                     VFSRKPLFGYQFVVFSGIAIGFIGFGVWAHHMFAAGLGPVANTAFGVTTAIIAVPTGV
                     KIFNWMGTIYGGDIRFTSAMLFTLGGIMMFVIGGLSGVTHSIVPSDYQQTDTYYIVAH
                     FHYVIFGGGVFAFFSAMFYWWPKVFGRLLNERLGVIEFVLMTIGFNLTFGPMHILGLQ
                     GMIRRTYTYPESLGLTFWNEAATVGAFLIALSVLVFIVNVVISHAKAKPSDVADPWDA
                     RTLEWMTTSPPPVHNFDEVPTVHALDEFWHRKYVEDERTGRLVPAQAGAANGDADGRG
                     TDGHGAKHGIHLPSPSYWPLVAALGMPVIGYGVLFSWWLVGVGFVIALIGFYGWAMEP
                     SVSPEGV"
     gene            3066..3710
                     /locus_tag="VF195_09450"
     CDS             3066..3710
                     /locus_tag="VF195_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-copper oxidase subunit III"
                     /protein_id="HEX6581082.1"
                     /translation="MADVAIAHGDAHGSEHPETTTGLPHTKLAMWIFLASECMLFGAL
                     ITTYVLYRGRPGQPPPHPNEIFDIPYTSVSSFVLLASSLTMVLALSAAQERDTGRMRL
                     WLLTTALLGMTFVGGQVYEFTAFYNEGLRLSTNLFSTTFYVLTGFHGVHVTVGVLMLL
                     SLVGLSSARRLPEDPSFPIEMVGLYWHFVDIVWIVIFTVVYLIPTPNPGGPGGP"
     gene            3700..4029
                     /locus_tag="VF195_09455"
     CDS             3700..4029
                     /locus_tag="VF195_09455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX6581083.1"
                     /translation="MALEQREVEVPAEHAGRHGGHPSPKEYVRIGVILGVLTALEIGA
                     SYAGVSTGILIPTLFILAIVKFALVVMWFMHLRFDDRRYARFFVMGLAGASVLYLIVL
                     ISLRVFA"
     gene            4029..4859
                     /locus_tag="VF195_09460"
     CDS             4029..4859
                     /locus_tag="VF195_09460"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEX6581084.1"
                     /translation="MALPPFHVHLDVLATLGGLAAVYLLAAGRHERTTGERLEPGQRH
                     RFLGGVAVLLMGATWPIHDLAESYLYSMHMVQHMLFTFVAAPLLLTGTPAWMWRAIFP
                     PGPLRTVWALLTRPLVALIVANGVLLFMHWPEIVSLSVRSELAHFSLHTLLLGSAIVM
                     WWPVLSPLPELPSLSRPGQLLYLFFQSLAPTIPASFLTFGTEPLYPIYATFPRIWGMN
                     ALTDQLVAGLVMKIAGGAILWVVIAAIFFRWGKEETTEGWDALKFRNVEREIRAGMGR
                     "
     gene            4856..5833
                     /locus_tag="VF195_09465"
     CDS             4856..5833
                     /locus_tag="VF195_09465"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6581085.1"
                     /translation="MSDRGERQSLLLPILVPIGALAAIVLVLFTFSRVLLSLKPNAAT
                     ATALVAAAGIMAVAAFVASRRSVTGTSLGAFVGASAGIAMLAGGIAIAVIGPPEDEVE
                     PFHATIAAPEGAIESGFSTDALTFEADVPVELEFDNADPGVGHNVQIFDGPDESAPSL
                     FDGTAITGPDAIPYAIAPLAPGEYFFHCRLHPGTAMQGTITVQKGAGGITVVTKDTAF
                     DTDEIRLPAETPSTLTLDNEDPFDHNLSIYEDDTASGEPLFTFEPFAGPASRPFDVPA
                     IPEGEYYFHCDIHPTMNGTVVVAPGPPPGEGGGAPPGEGSPSASPDEGG"
     gene            5857..6384
                     /locus_tag="VF195_09470"
     CDS             5857..6384
                     /locus_tag="VF195_09470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6581086.1"
                     /translation="MPRPNAPILILALLALLALLALLGSCRADDSVFGVAEQDAPLPR
                     LAGETVDGDHLDAGSYADRSVLVINVWADWCAPCRREQPQLVRLADRYEEEGVRFLGI
                     NYQDDRDAAKAWIEEFGVPYPSLFDPSGRSAVDLGFPALPDTYVVDRSGTIRWVVYGE
                     TDERELASLIDDVLA"
     gene            complement(6389..8206)
                     /locus_tag="VF195_09475"
     CDS             complement(6389..8206)
                     /locus_tag="VF195_09475"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF003349.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEX6581087.1"
                     /translation="MVFTHSRFRSLALVTAIATIALFAIGGLVRGTGSGLGCSTWPAC
                     EPGHLFPSGTIHSLIEFSHRTMAFLVAVLAALTGVTAIKTARERPRLLWPAVLAFPLV
                     LAQAGVGAVVVATELDPWWVTVHFVAALLLIADVTYVAAAVFPGVETRNEVGTGARPF
                     SRLALPLATIVGALLLVGTYVRASDAQLVFTDWPLMDGRLVPTLGGAATAMFLHRGLA
                     AIAMLLVLWTAIRARTDPGRRDVLVRLSSIALVLFAAQILVGAANVWTRLRPWAVVAH
                     VALSVLIWATVVALATASRLSDASTEPEPGPAGQATGNRPSLRDTVTAYYRLTKPRIV
                     LLLLITTVPAMLLAAEGLPSPWLILATLSGGALAAGSANAINMYLDRDIDAIMRRTRQ
                     RPLPAHAIAPEHALRFGFVLGAIAFYFLAVAVNVLAAVLALSAIAFYVFVYTMWLKRT
                     TAQNIVIGGAAGAVPALVGWAAVTGGLAWPAIVLFAIVFVWTPPHFWALAMRFSGDYA
                     AAGVPMLPVVRGEDETRRQILLYSLVLFATTLVLVPVGHMGPIYTGAAVLLGGSFVYR
                     SLRVWRSAEEARTRRLFSFSILYLAGLFGAVGADALLHA"
     gene            8266..9132
                     /locus_tag="VF195_09480"
     CDS             8266..9132
                     /locus_tag="VF195_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6581088.1"
                     /translation="MTGDLRSRVDAVLTTFLDERLRAVDRLDPAARPPIQEIGRLLAA
                     GGKRIRPVFCYWGFRAAGGEDRGTRDEPIVHAAAALELLHTMALVHDDLIDGAKERRG
                     VPSTAAWFAERAEELEAKGEPTAFGEAMAVLVGDLSAVLADHLLLESGFSPDALADAL
                     AVYHPMREEMAIGQFLALAGSGHAVAPDRARRVADLKGGGYTVEGPLRIGAALAGGTD
                     AAGACLSRFGRPLGEAFQLRDDLTDGDAAPGVTLETVNERIADAKAALDPEVLVAEAV
                     PILRGLADEVAA"
     gene            9129..9554
                     /locus_tag="VF195_09485"
     CDS             9129..9554
                     /locus_tag="VF195_09485"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6581089.1"
                     /translation="MTEDLEMLRRLFRDQPACRVATVRPHGGPHVATRWFVWMPDALY
                     VATRVGDTTWEALEHDARVSLLVDRGREWNDLSGVRIEGVAELLPAEHPDLRQPMSAW
                     HEKYRTLFAGDGFERFTAAIPKLGFLRVVPAVVDAWDHG"
BASE COUNT         1455 a         3263 c         3361 g         1580 t
ORIGIN      
        1 gtcctcagaa gagctccggg agaccgaagg aaccgtgcgg aagaggttgc tctgggcgac
       61 ggccgggatc ggcctcctcc tgctgttgag cggctgtgca cccgacgcca cgcaggacac
      121 cctccagccg caggggccgt acgcccagaa gctgaaagac ctcttcatcc ccgtcttctg
      181 ggtggcggtc ttcgtcttca tcgtcgtcga gggcggcatc gtgtggatcg cgatccggta
      241 ccggcatcgc aagggccgcg agcgcatgcc ggcgcagatc cacggcaaca cgcgcctcga
      301 gatcggctgg acgatcgctc cggcgctgat cctggccggc gtcatggtac ccaccgtcgc
      361 gacgatctgg gatctcgcac ggaagccgcc ggacgacgcg atcaacgtca cggtgcgcgg
      421 gctccagtgg tggtgggact tcgagtacac ggacgacgac atgcgcacaa cgtacgggga
      481 ccaggcgtcg atcaggacgg cggacgtcct ggtgatcccg gaggatcggg tgatctacct
      541 gtccctcgag ggagccggcg gagacatcgg cggggaggat gacgaggcca aggactacat
      601 ggtgatccat tccttctgga tcccggagct cgccggcaaa caggacgtcg tgcccggtcg
      661 aacgaaccac atcctgatgc aggcggacca cccgggtatg tacgagggac agtgcgccga
      721 gttctgcggt ctccagcacg ggaagatgct cgtgcgggtg cgcgcgctct cggccgagga
      781 ctggcaggcc tgggtcgaga accagaagcg agcgtcggcc gagccgaccg gtctcgccgg
      841 ggacgggatg gatctgttcc tcgcaccact gtccgacggt cgcggttcct gcgtcacgtg
      901 tcacgccgtg ggggggaccg aggccaccgc cctcgcggga ccggacctca cgcacttcgc
      961 cgatcccacg cacgagtgtt tcgcgggctg caactgggaa acgacggatc gcgaggcgtt
     1021 ggaggcctgg ctcaggaacc cggacgcggt gaagctgggg tcgaagatgc ccaattacca
     1081 tctgagggaa gacgagatcg acgccctcgt ggcctacctg tacagcctga cgtgagcacg
     1141 cacgcgagac gacggagtgg atgacggatg gcgaccgtga gcagcagcgg gcgacgcggt
     1201 gggatcctgc gacgcccgac ggcgctgacc gggttctcga gctggttcac gaccatcgac
     1261 cacaagaaga tcgggttcct ctacggggcg acggcgttcg tgttcttcat cgtcggaggt
     1321 gtcgaggcgc tgctgatccg cgcccagctc gcgcagcccg acggcacgct gctgaacgcc
     1381 gaccagtaca gcgaggtctt cacgatgcac gggatcacga tgatcttcct gttcgtgatg
     1441 ccgatggcgg cggcgttctt caactacttc atcccgctga tgatcggcgc gcgggacgtc
     1501 gcgttcccac ggttgaacgc gtacagctac tgggtgttcc tgctcggcgg gctcttcctc
     1561 tactcgagct tcgtcctggg gggagcgccg aacggtggct ggttcggcta cgcgccgaac
     1621 tcgaccaccg atccgtcgat cgggatgagc ttctatgcga tcgggctgat catcaccggt
     1681 atcgcctcga cggcgggagc cgtcaacctc gccgtcacgg tcatcaatat gcgggcgccg
     1741 gggatgtccc tgttccgcgt gcccgtgttc gtgtggatgg ggctggtggt gcagctgctt
     1801 ctcgccttct ccctgccgat catcacggtg gcgctgttcc agcttttgtt cgaccgtgag
     1861 ctggggaccc acttcttcga tccgatggcg ggtggtgatc ccatcctgtg gcaacacctc
     1921 ttctggctct tcgggcatcc cgaggtctac gtgctgatcc tccccgcctt cgggatcttc
     1981 tcggaggtgc tgccggtctt ctcgcggaag cccctgttcg ggtaccagtt cgtggtgttc
     2041 tcggggatcg cgatcggctt catcgggttc ggcgtctggg cccaccacat gttcgcggcc
     2101 gggctggggc cggttgccaa caccgccttc ggcgtcacga cggcgatcat cgcggtgccc
     2161 accggcgtca agatcttcaa ctggatgggc accatctacg gtggcgatat ccgcttcacg
     2221 tcggcgatgc ttttcacgct gggcgggatc atgatgttcg tgatcggcgg gctgtccggc
     2281 gtgacgcact cgatcgtccc gtccgattac cagcagaccg acacctacta catcgtcgct
     2341 cacttccact acgtgatctt cggaggtggt gtcttcgcct tcttcagcgc gatgttctac
     2401 tggtggccca aagtgttcgg gcgcctcctg aacgagcggc tcggcgtgat cgagttcgtc
     2461 ctgatgacga tcgggttcaa cctgacgttc gggccgatgc acatcctcgg cctgcagggg
     2521 atgatccggc ggacctacac gtacccggag tcgctcggtc tcacgttctg gaacgaagcc
     2581 gcaacggtcg gcgcgttctt gatcgccctc tccgtcctcg tgttcatcgt caacgtcgtg
     2641 atcagccacg cgaaggcgaa gccctccgac gtcgccgatc cctgggacgc ccgaaccctc
     2701 gaatggatga cgacctcccc gccacccgtc cacaacttcg acgaggtgcc cacggtccac
     2761 gcgctggacg agttctggca ccgcaagtac gtcgaggacg agcggacggg ccggctcgtc
     2821 ccggcccagg ccggtgccgc gaacggcgac gccgacgggc gcggaacgga cggacacggc
     2881 gcaaaacacg gcatccacct gcccagcccc tcgtactggc cgctcgtggc ggcgctcggg
     2941 atgccggtga tcggctacgg cgtgctcttc tcatggtggc tcgtcggagt cgggttcgtg
     3001 atcgcactga tcggcttcta cggatgggcg atggagccct cggtgtcgcc ggagggcgtg
     3061 tagcgatggc tgacgtcgcg atcgctcacg gtgacgccca cggcagcgaa cacccggaga
     3121 ccaccactgg gctgccgcac acgaagttgg cgatgtggat cttcctcgcg tccgaatgca
     3181 tgctcttcgg cgccctgatc acgacgtacg tgctgtatcg agggcggcct gggcaaccgc
     3241 cgccccaccc gaacgagatc tttgacatcc cctacacgtc ggtctcctcg ttcgtcctgc
     3301 tcgcgagctc gctcacgatg gtgctcgcgc tgtccgcggc gcaggagcgc gacaccgggc
     3361 ggatgcggtt gtggttgctc acgaccgcgc tgctcggcat gacgttcgtc gggggtcagg
     3421 tctacgagtt cacggccttc tacaacgagg gcctcaggct ctcgacgaac ctcttctcga
     3481 cgaccttcta cgtcctgacc gggttccacg gggtccacgt caccgttggc gtcctgatgc
     3541 tgctctcgct cgtcgggctg tcgtcggccc ggcgcctgcc ggaggatccc tcgttcccga
     3601 tcgagatggt gggcctgtac tggcacttcg tcgacatcgt ctggatcgtg atcttcacgg
     3661 tcgtctacct gatcccgacg ccgaacccgg gaggccctgg tggcccttga gcaacgagag
     3721 gtcgaggtac cggccgagca cgcggggagg cacggtggcc atccgagccc caaggagtac
     3781 gtccggatcg gcgtgatcct cggcgtcctc acggccctcg agatcggcgc gtcgtacgcg
     3841 ggggtgagca cgggcatcct gatcccgacg ttgttcatcc tggcgatcgt gaagttcgcc
     3901 ctggtcgtga tgtggttcat gcacctgaga ttcgacgatc gccgctacgc acgcttcttc
     3961 gtgatgggtc tggccggcgc gtcggtgctc tacctcatcg tgctgatctc gctccgggtc
     4021 ttcgcctgat ggccctgcct ccgttccacg tgcacctcga cgtgctcgcg acgctcggcg
     4081 ggctcgccgc cgtctacctg ctggccgcgg ggcgccacga gcggaccacg ggagagcggc
     4141 tggagcccgg gcagcggcac cgcttcctgg gaggtgtggc cgtgctgctg atgggtgcga
     4201 cctggccgat ccacgatctc gccgagagct acctctacag catgcacatg gtgcagcaca
     4261 tgctgttcac gttcgtggcc gcaccgctgc tgctcacggg gacgccggcc tggatgtggc
     4321 gcgcgatctt cccacccggt ccgttgcgga ccgtgtgggc gctgctcacc cgtccgctcg
     4381 tggccctgat cgtcgccaac ggcgtgctcc tcttcatgca ctggcccgag atagtctccc
     4441 tctccgtccg ctccgagctc gcgcacttct cgctgcatac gctgctcctg ggctccgcga
     4501 tcgtgatgtg gtggccggtc ctgtcgcccc ttcccgagct cccctcgctg tcgcgaccgg
     4561 ggcagctcct ctacctgttc ttccagtccc tcgcgccgac catcccggcc tcgttcctga
     4621 cgttcggcac ggagccgctc tatccgatct acgccacctt cccccggatc tggggcatga
     4681 acgcgctcac cgatcagctc gtggccggcc tcgtgatgaa gatcgccggc ggcgccatcc
     4741 tctgggtcgt gatcgcggcg atcttcttcc gttggggcaa ggaggagacg accgagggct
     4801 gggacgcctt gaagttccgc aacgtcgaac gagagatccg cgcggggatg ggtcgatgag
     4861 cgatcgcggc gaacggcaga gcctgctcct gccgatcctg gtcccgatcg gggcgctcgc
     4921 ggcgatcgtc ctcgtcctgt tcacgttctc ccgagtgctg ctgtcgctca agccgaacgc
     4981 cgccacggcc accgcgctcg tcgcggccgc ggggatcatg gccgtcgccg cgttcgtcgc
     5041 gtcgcgcagg agcgtcaccg ggacgtccct cggcgcgttc gtgggcgcct ccgccggcat
     5101 cgcgatgctc gccggcggga tcgcgatcgc ggtgatcgga ccgccggagg atgaggtcga
     5161 gccgttccac gcgacgatcg cagcccctga gggagcgatc gagagcgggt tctcgacgga
     5221 cgcgttgacg ttcgaggccg acgtccccgt cgagctcgag ttcgataacg cggacccggg
     5281 cgtgggacac aacgtgcaga tcttcgacgg gcccgacgag tcggcgccct cgctgttcga
     5341 cggcaccgcg atcaccgggc ccgacgcgat cccgtacgcg atcgccccgc tcgctccggg
     5401 ggagtatttc ttccactgcc gactgcaccc ggggacggcg atgcagggga cgatcaccgt
     5461 ccagaagggt gcgggcggaa tcacggtcgt gacgaaggac accgcgttcg acaccgatga
     5521 gatccggctg ccggcggaga cgccgtcgac gctcactctg gacaacgagg atccgttcga
     5581 ccacaacctg tcgatctacg aggacgacac ggcatccggt gagcccctgt tcacgttcga
     5641 gccgttcgca ggtccggcgt ccaggccgtt cgatgtgccg gcgatcccgg agggcgagta
     5701 ctacttccat tgcgacatcc accccacgat gaacggaacg gtcgtggtgg cgccgggtcc
     5761 gccgccggga gagggtggtg gggctccgcc cggcgagggg agtccgagcg cttcgcccga
     5821 cgagggcggc tgagacccac tgatagcgtg gccgccgtgc cacgtccgaa cgctcccatc
     5881 ctgattctcg cgctcctcgc gctcctcgcg ctcctcgcgc tcctcggatc ctgcagggcg
     5941 gacgactcgg tgttcggcgt cgccgagcag gatgcgccgc tgccgcgact cgcgggcgag
     6001 accgtcgacg gcgaccacct ggacgcgggg tcctacgcgg accgcagcgt cctcgtgatc
     6061 aacgtgtggg ccgactggtg cgccccgtgc aggcgggagc agccgcagct cgtgcgactg
     6121 gcggatcgat acgaggagga gggcgtgcgg ttcctgggga tcaactacca ggacgatcgc
     6181 gacgcggcca aggcatggat cgaggagttc ggggtgccgt atcccagcct gttcgacccc
     6241 tcgggccgct cggccgtcga cctcgggttc ccggccctgc cggacaccta cgtcgtggac
     6301 cgcagcggaa cgatccgatg ggtggtgtac ggtgagacgg atgagcgcga gctcgcgagc
     6361 ctgatcgacg acgtgctggc ctgacacctc acgcgtggag caaggcgtcc gcgccgacag
     6421 cgccgaagag tccggcgagg tacaggatcg agaacgagaa caagcgtctc gtccgggctt
     6481 cttcggcgct tcgccacacc cgcagcgagc ggtagacgaa cgagcctccg agcaggacgg
     6541 cggcgccggt gtagatgggc cccatgtgtc cgaccggcac cagcaccagc gtcgtcgcga
     6601 agaggacgag cgagtagagg aggatctgcc gccgggtctc gtcctcgccg cggacgaccg
     6661 gcagcatcgg gacgcccgcc gcggcgtaat cgcccgagaa ccgcatcgcg agcgcccaga
     6721 agtgcggcgg cgtccagacg aagacgatcg cgaacaggac gatcgccggc caagcgaggc
     6781 ccccggtgac ggccgcccat ccgacgagcg ccggcacggc gccggccgcg ccgccgatca
     6841 cgatgttctg cgccgtcgtg cgcttcagcc acatcgtgta cacgaagacg tagaaggcga
     6901 tcgccgacag cgcgagcacc gcggccagca cgttcaccgc gacggcgagg aagtagaagg
     6961 cgatcgcgcc caggacgaag ccgaaacgca gggcgtgctc gggggcgatc gcgtgggcgg
     7021 ggagcggccg ctgccgcgtc cgtcgcatga tcgcgtcgat gtcccggtcg aggtacatgt
     7081 tgatggcgtt cgcggagccg gccgcgaggg cccctcccga cagcgtggcc aggatcagcc
     7141 acggcgaggg gagtccctcg gcggcgagca gcatcgccgg caccgtcgtg atcagcagca
     7201 gcaggacgat gcgcggcttc gtgagccggt agtaggcggt cacggtgtcg cggagcgagg
     7261 gacggttgcc ggtcgcctgc cccgccgggc cgggttccgg ttcggtggaa gcgtcgctca
     7321 gcctcgacgc ggtggcgagc gccacgaccg tggcccagat caggacggac agcgccacgt
     7381 gcgccacgac cgcccagggc cggagccgcg tccacacgtt ggccgcgccg accaggatct
     7441 gagcggcgaa gagcaccagg gcaatcgagg agagcctgac gaggacatcg cgacgtccgg
     7501 ggtccgtccg tgcccggatc gcggtccaga gcacgagcag catcgcgatc gcggcgagcc
     7561 cgcggtgcag gaacatggcc gtggccgcgc cgccgagcgt cggcacgagc ctgccgtcca
     7621 tcagcggcca gtccgtgaac acgagctgtg cgtcgctagc gcgcacgtac gtgccgacga
     7681 ggagcaacgc cccgacgatc gtcgcgagcg gaagcgcgag ccgcgagaac ggtcgggcgc
     7741 cggtccccac ctcgttccga gtctcgacgc ccgggaacac ggcggccgcg acgtacgtga
     7801 cgtcggcgat caacaggagc gccgccacga agtgcaccgt gacccaccac gggtcgagct
     7861 cggtcgcgac gacgaccgcg ccgacccccg cctgcgccag cacgagcggg aaggcgagga
     7921 ccgccggcca caggagccgg ggtcgctctc tcgcggtctt gatcgcggtc acgccggtga
     7981 gcgctgccag caccgcgacg aggaacgcca tcgtgcgatg ggagaactcg atcagtgaat
     8041 ggatcgtccc cgaggggaac aggtggccgg gctcacaggc cggccaggtg gagcatccga
     8101 gcccggagcc cgtcccgcgg acgagtccac cgatcgcgaa cagcgcgatc gtcgcgatcg
     8161 ccgtgacgag cgcgagggac cggaagcggg agtgggtgaa gaccatcgcg acgtcggcat
     8221 gatagtcgcg gcccggtgcg ccctcggcct tgcgatactg ccgcggtgac cggggatctc
     8281 cgctcgaggg tcgatgccgt gctcaccacc ttcctcgacg agcggctccg cgccgtggac
     8341 cggctcgacc cggcggctcg cccgccgatc caggagatcg ggcgtctcct ggcggctggc
     8401 gggaaacgca tccggcccgt gttctgctac tggggcttcc gcgcggcggg cggcgaggat
     8461 cgcgggaccc gcgacgaacc gatcgtgcac gccgcggcgg cgctcgagct cctgcacacg
     8521 atggcgctcg tccacgacga tctgatcgac ggggcgaagg agcggcgggg ggttccctcg
     8581 acggccgcgt ggttcgcgga gcgagcggag gagctcgagg cgaaggggga gcccaccgcg
     8641 ttcggcgagg cgatggcggt gctcgtcgga gacctgtcgg ccgtgctcgc ggatcacctg
     8701 ctgctggagt cggggttctc accggatgcg ctggcggatg cgctcgccgt gtaccacccg
     8761 atgcgggagg agatggccat cggccagttc ctcgcgctgg cggggtcggg ccacgccgtg
     8821 gccccggacc gggctcgccg cgtcgccgac ctcaagggcg gcgggtacac cgtggagggc
     8881 ccgttgcgga tcggggccgc ccttgccggc gggaccgacg ccgcgggggc gtgcctttcg
     8941 cggttcgggc gtccgctcgg cgaggccttc cagctccggg acgatctcac ggacggcgac
     9001 gcggcgccgg gcgtcaccct cgagaccgtc aacgaacgca tcgccgacgc gaaggctgcc
     9061 ctcgatccgg aggtcctcgt cgcggaggcg gttcccatcc tccgtgggct cgcggacgag
     9121 gtggcggcgt gaccgaagac ctcgagatgc tgcggcggct cttccgcgac caaccggcgt
     9181 gccgggtggc gacggtgcga ccccacggcg ggccgcacgt cgcgacccgg tggttcgtgt
     9241 ggatgccgga tgcgctctac gtcgcgaccc gtgtcggcga cacgacatgg gaggcgctcg
     9301 agcacgacgc ccgcgtctcc ctcctcgtcg atcgcggccg cgagtggaac gacctctccg
     9361 gcgtgcggat cgaaggcgtc gccgagctcc tgcccgccga gcatcccgac ctgcgccagc
     9421 cgatgtccgc ttggcacgag aagtaccgga ccctcttcgc gggcgacggg ttcgagcgat
     9481 tcaccgccgc gatcccgaag ctcgggttcc tccgggtcgt ccccgccgtc gtggacgcgt
     9541 gggaccacgg ctgagacctc cgtgcgatag ggtgcttgtg aaaggtttca cgagcgcccg
     9601 acggggctcc tgaggggagg cgcgcgtgcc cgagcggatc gagaccgacg tcctgatcg
//
LOCUS       DASUYD010000164         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_756081, whole genome shotgun sequence.
ACCESSION   DASUYD010000164 DASUYD010000000
VERSION     DASUYD010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756081"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1443
                     /locus_tag="VF195_09490"
     CDS             325..1443
                     /locus_tag="VF195_09490"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6581090.1"
                     /translation="MGPRLGGGAVPVGRRNLLADRRRLTISVGGVAAAIALILLLQGL
                     WSGFQVQISAYEDNVGADLFVGEPGTANFLGVPSVLPLETARAIRAVPGVERADPIAV
                     RFAVLDLHDRKVFMFLVGSRPEGMGGPWDLAAGKPARADDEVVMDETVADQHGIALGD
                     RVEVMGELFKVVGLSTDTRSWMAGFVFTTHHAIERLLRMPGTTSYILVRAADSEAVAG
                     SIRDQTGLAVLTPQRLGDNDRALLGRIMNGAIGLMLVIAFAAGTLIIALTVYSAIVER
                     LREYGIAKAMGARGARLFRIVLGQTLTLSALGTLAGYVLFRGASWLVVMVRPQFWVRV
                     SWTGVAGALLAAGLMAFLAAVVPTRRITRLDPASVYRG"
     gene            1448..2554
                     /locus_tag="VF195_09495"
     CDS             1448..2554
                     /locus_tag="VF195_09495"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581091.1"
                     /translation="MRAVPIGRRNLFSDRRRAILGVAGVAVALLLTLALDGIFAGAMR
                     QVTRYIDESGADLFVSQRGVRTMHMSSSAIPLTSVEEIRGMPGVRWADPILYDTGAVL
                     ASGKRQLTYLIGYVPGRRGGASRLAAGTEPGDGEIVLDEDAAATLGVGVGGRVEILER
                     TWRVSGLATQMTNIVNSVSYVPFQDFADARGVTDVASYVLVGAQGSIESVRAAVEAVT
                     GLTAQTRVAFASQERQGIKDMSTDLLQIMTFAAFLIGIAVIGLTLYAATLSRLHEIGV
                     MKALGGTSMLLSRSVLSQAAWTVGLALAAGWLAALGFGWLLGRTGGSFPIVIEAGSVV
                     RAGIGAMLLAALGAVVPLIKVSRVDPATVFRRKS"
     gene            2551..>3155
                     /locus_tag="VF195_09500"
     CDS             2551..>3155
                     /locus_tag="VF195_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013882194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581092.1"
                     /translation="MNDIAIKAEKLEKVYGEGDVQVRAVRGVDLEVRRGEVILIMGPS
                     GSGKTTLLSMLGAMLRPTSGQVEVDGVDLAALPERRLPEFRALKFGFVFQDFNLLSAL
                     TARENVEFALNLAGTTGARARDRAEEILQAFGLSARLHFRPGKLSGGEKQRVAIARAL
                     VNEPAVILADEPSANLDSKIGHEIARLLRRAAEEGRSVIIV"
BASE COUNT          449 a         1030 c         1140 g          536 t
ORIGIN      
        1 cgacgtgggt cccagcatgg cggtccagat gcctggagac ggcatgctga aacgatcttc
       61 gagcgaggca tacgtcgaag ccctggagca gcacacccgg acgtacaaca agatggccgg
      121 gctcacgccg taggcgagcc cgtcgccggc gagcctggat tcttccgtcg ctcctcaacg
      181 gagatctccc tcgctccgaa gggggcgtcc gtgacccatg tccgaccgcg gaccggacgg
      241 cccgtgggag tggggacctt cgtacctatg agccgcgagt gcccccctgt agccttaccc
      301 ccgtagggta tagaagggaa gacgatgggc ccgagacttg gtggtggagc ggttccggta
      361 ggccggcgga acctgctcgc cgaccgacgt cgcctgacga tcagcgtggg cggcgtcgcc
      421 gcggcgatcg ctctgatcct cctgctccaa ggtctgtgga gcggcttcca ggtgcagata
      481 tccgcgtacg aggacaacgt cggggccgat ctcttcgtgg gtgaaccggg cactgccaac
      541 ttcctgggtg tgccatcggt cctgcccctg gagaccgctc gggcgatccg ggccgtccct
      601 ggggtggagc gcgccgaccc catcgcggtg aggttcgcgg tgctcgacct ccacgatcgc
      661 aaggtcttca tgttcctggt cggctcccgt ccggagggga tgggcgggcc ctgggatctg
      721 gccgcgggca agccggcccg ggccgacgac gaggtggtca tggatgagac ggttgccgac
      781 cagcatggga tcgccctcgg tgaccgggtc gaggttatgg gggagctgtt caaggtggtg
      841 gggctctcca cggacacgcg ctcgtggatg gctggcttcg tgttcaccac gcaccatgcg
      901 atcgagcggc tgcttcggat gccggggacc acgagctaca tcctggtccg ggccgcagac
      961 tccgaagccg tcgccgggag catccgcgac cagacggggc tcgccgtcct cacgccccag
     1021 cggctcgggg acaacgaccg ggcgctcctc ggccggatca tgaacggtgc gatcggattg
     1081 atgctggtca tcgccttcgc cgcgggcacc ctgatcatcg ccctgaccgt gtactcggcg
     1141 atcgtggagc gactccgcga gtacgggatc gccaaggcga tgggggcgcg aggggcacgg
     1201 ctgttccgga tcgtccttgg ccagacgctc accctctcgg cgctcgggac cctcgccggc
     1261 tacgtgctgt tccgcggcgc ctcgtggctg gtcgtcatgg tccgtcccca gttctgggtg
     1321 cgggtctcgt ggacgggagt tgccggcgcg ctgctggccg ccggtctgat ggcgttcctg
     1381 gcggcggtcg ttcccacgcg ccggatcacg cggctcgatc cggcctcggt gtaccggggg
     1441 tgatgatgtg cgagcggtac cgatcggacg gcggaacctc ttctccgatc gccggcgggc
     1501 gatcctcgga gtggcaggtg tggcggtcgc cctcctcctg acgttggctc tcgacggcat
     1561 cttcgccggg gccatgcgcc aggtgacccg gtacatcgac gagtcgggtg ccgacctgtt
     1621 cgtctcccag cgtggcgtac gcacgatgca catgtcatct tccgcgatcc ccctcacgtc
     1681 ggtcgaggag atccgcggga tgcccggtgt ccgttgggcg gacccgatcc tgtacgacac
     1741 cggcgccgtc ctggcttcgg ggaagcgcca gctcacgtat ctcatcggtt acgtgccggg
     1801 ccgccggggc ggtgcctcca gactcgccgc ggggacggag cccggtgacg gggagatcgt
     1861 cctcgacgag gatgcggcgg ccaccctcgg ggtcggcgtg ggcggccgcg tggagatcct
     1921 cgaacggacg tggcgtgtct cggggctcgc gacccagatg accaatatcg tcaactccgt
     1981 ctcctacgtg ccgttccagg acttcgctga tgcgcgagga gtgaccgatg tagcgagcta
     2041 tgtcctggtt ggggcgcaag gctcgatcga gtcggttcgg gccgccgtcg aggccgtcac
     2101 cggccttacc gcccagaccc gcgtcgcctt cgcctcgcag gaacggcagg gcatcaagga
     2161 catgagcacc gatctgttgc agatcatgac cttcgccgcg ttcctcatcg ggatcgccgt
     2221 gatcggactc acgctgtacg cggccacgct gtcgcggctg cacgagatcg gcgtgatgaa
     2281 ggcgctcggc gggacctcga tgctgctgag ccggagcgtg ttgtcgcagg cagcctggac
     2341 cgtcgggctc gcgctggccg ccggatggct cgccgcgctc ggcttcggat ggctgctcgg
     2401 gcgcaccggc gggagcttcc cgatcgtgat cgaggccggg tccgtggtga gggccgggat
     2461 cggcgcgatg ctccttgccg ctctgggggc cgttgttccc ctcatcaagg tgtcgcgggt
     2521 cgaccccgcg accgtcttcc ggaggaagtc atgaacgaca tcgcgatcaa ggcggagaag
     2581 ctcgagaagg tgtacgggga aggtgacgtg caggtgaggg cggtgcgcgg cgtcgacctg
     2641 gaggtccgcc gtggagaggt gatcctcatc atgggaccgt cgggaagcgg gaagacgacg
     2701 ctgctctcga tgctcggcgc gatgctccgc ccgacgtccg ggcaggtgga ggtggacggg
     2761 gtggacctgg ccgcgctccc ggaacggcgc ctccccgagt tccgcgcgct caagttcggg
     2821 ttcgtcttcc aggacttcaa cctgctgtcg gcgctcacgg cacgggagaa cgtggagttc
     2881 gccctcaacc tcgccggaac gaccggagcg agggccaggg accgcgccga ggagatcctc
     2941 caggcgttcg gcctcagtgc gcgcctccac ttccgacccg gcaagctctc gggcggcgag
     3001 aagcagcgcg tcgccatcgc ccgagcgctc gtgaacgagc cggccgtcat cctcgccgac
     3061 gagccgagcg cgaacctcga ctcgaagatc ggccacgaga tcgcccgcct cctgcggcgg
     3121 gctgcggagg aggggcggag cgtgatcatc gtgag
//
LOCUS       DASUYD010000165         8552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1860250, whole genome shotgun sequence.
ACCESSION   DASUYD010000165 DASUYD010000000
VERSION     DASUYD010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8552
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1860250"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..199
                     /locus_tag="VF195_09505"
                     /pseudo
     CDS             1..199
                     /locus_tag="VF195_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182253.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(403..1152)
                     /locus_tag="VF195_09510"
     CDS             complement(403..1152)
                     /locus_tag="VF195_09510"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEX6581093.1"
                     /translation="MKSIIGIERFLEGPKYELRRRPESPAPAAHRPVITISRQAGSGA
                     HVVAEALVAGLQASDPQASPPWTMFDRDLVERVLQDHKLPDRLAEFMPEDRVSGIADT
                     IDEILGVHPPRWTLVRKTADTILRLAEIGNVVVIGRGGNVITDRLAYALHVRLVGSVP
                     RRIQHLQDYLHVGPAEAAEYLRTRDAARKRYVAKYYDRDIDDPLLYHLVVNTDRVTYG
                     KAARLILGAVHERHDRTARAGATPRAVAPAI"
     gene            complement(2120..2428)
                     /locus_tag="VF195_09515"
     CDS             complement(2120..2428)
                     /locus_tag="VF195_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6581094.1"
                     /translation="MPGDPDLQLCVWIRLRHLRGDDEESDRGCRRPPGRALVEARAEA
                     REVQIETIAAEGQPAKVLVEVAKGANLLVVGSRGFGGFQELLLGSVSQQCANTPRAQS
                     "
     gene            complement(2364..2537)
                     /locus_tag="VF195_09520"
     CDS             complement(2364..2537)
                     /locus_tag="VF195_09520"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6581095.1"
                     /translation="MSGRVVVGVDGSETARKAVRWAAREAKLRGMKLELVSAWRSRPT
                     AMCLDTASSSPRR"
     gene            complement(2875..3168)
                     /locus_tag="VF195_09525"
     CDS             complement(2875..3168)
                     /locus_tag="VF195_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581096.1"
                     /translation="MYPPAGRIGFLSQSGALALAVTQHAIKRGLGPSSFVPMGNKADI
                     SGDDLLAHWSLDEATDVSLLYLESFGNARRFARLARKIGRPPARPLAARSGMD"
     gene            complement(3512..4027)
                     /locus_tag="VF195_09530"
     CDS             complement(3512..4027)
                     /locus_tag="VF195_09530"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEX6581097.1"
                     /translation="MSFVTEYLEARGIPYEVIHHDPSTTSLEEARSLHMDADHVLKAV
                     LIDTRGRHAVAVIPASRRLDMYLVRGALDDQAARLATEDELQGDFPSIELGATPVVGL
                     LLDAETLVDPSLVACDTVVFAAGTRTESVRVRTRDLFRDEHAAFVPLAAELEPSETLP
                     QSEDGSSISCS"
     gene            4227..5654
                     /locus_tag="VF195_09535"
     CDS             4227..5654
                     /locus_tag="VF195_09535"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018385733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX6581098.1"
                     /translation="MPSVPLVQEEPVRWRIQRHGAMRVPGIVFASKTLLPDVLSDRSL
                     TQVVNVAALPGIVEASYAMPDIHWGYGFPIGGVAATDVERGGVVSPGGVGFDISCGVR
                     LLASPFDRDELAPSFEALMDELDETIPRGLGGGGVWRLRDEELNEILVGGSRFAVEQG
                     FGVERDLERCEDGGAVGGADPGHVSQRAMQRGFRQVGSLGSGNHFLEVQVVEDVHDPA
                     VAAAFGLRAAQVCVMIHCGSRGLGHQICTDHVRTMLAAMPRYGIEVPDRQLACAPVDS
                     PEGRAYLGAMAAAANYGRANRHLLAEAARDAFASATGRRELDLVYDVSHNLAKPEEHV
                     VDGERRLLCVHRKGATRALPPGDRDLPPSLRAAGQPVLIPGSMGTASYVLTGVAGGGA
                     FHSTCHGAGRTMSRHAAKRRVQGATLRAELEARGIAVRARSIRELPEEAPFAYKDIDE
                     VVSTCEQAGLCARVARLSPIGVIKG"
     gene            complement(5661..6095)
                     /locus_tag="VF195_09540"
     CDS             complement(5661..6095)
                     /locus_tag="VF195_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX6581099.1"
                     /translation="MNERPSPACGHRVLPHTADVIIEAWGTSRLECLEEAVRALVDSF
                     ARVAVAATKPVPIEFDEHADEALLVSLLDEVIYVIDVLGGVPADLALDQTEDGRVVGV
                     IDLAPLSAVEIHGSPPKGVSLNELSITREDEGRWRCRATIDV"
     gene            complement(6543..7574)
                     /locus_tag="VF195_09545"
     CDS             complement(6543..7574)
                     /locus_tag="VF195_09545"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX6581100.1"
                     /translation="MEGSSGPRAPLRVGKLPARLLADLLADLPVPPADVLLGPHIGED
                     ACAIQVPGGVLVAATDPITFTAREIGRFSVIVNANDVAVMGVRPRWFLAVILLPSGST
                     EAMVRDVFADMRDGLAHVGASLVGGHTEVTPAVTQPLVVGQFLGIAEDGKFVTTGGMR
                     PRDVLLQVGPAPIEGAGVLAREAADRLTGLDRGDLDKALVAPDHPGISVVEPALLAAE
                     LGASAMHDPTEGGLAAGLHEMAEASGVRIRVDLSSVLWFSPGRAICDALAADPMATLA
                     SGALLAAFPPDRAGPAMRALARHGYTASDIGRAGDGSGVCDADGRPLAWPERDEVARL
                     LAGETDAHG"
     gene            8374..8529
                     /locus_tag="VF195_09550"
     CDS             8374..8529
                     /locus_tag="VF195_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581101.1"
                     /translation="MRLMAAGRCHWRTPIPITYQTDLGKGYAQFEILALYFSDVKIVD
                     VSGRRAD"
BASE COUNT         1390 a         2911 c         2849 g         1402 t
ORIGIN      
        1 ggaaggtcga gcggtcccac cggatcgatg ccgaggagtt ctatcggatg ctcgacgggg
       61 gttgtgatcg atgacaccgg cctgttcaac actcgcctga aagagtggga ggacttctac
      121 aacttccatc gaccgcatgg acctcggggg ccagacgccg tatgagaggc tgcgggagaa
      181 gacgacagcc cgggcgtgaa cggacccgtc agctgcacag ccagccgagg aacttcactg
      241 cgtgctgccc gagcgagacc tttgcggcct ctcgcccagg cgcggccggc gctcctcgag
      301 gttgactcgc cgtcgcggag ccatccgcta cgtgtccctc cgagcgggtt tcacgcgcgg
      361 ttcgcgagac gcagcggtcc ccggagccga ccggtcacgc tcctagatcg ccggtgcgac
      421 ggcacgaggg gtcgcaccgg cacgtgcggt tcggtcgtgg cgctcgtgca ccgccccgag
      481 gatcagtcga gcggccttcc cgtacgtgac tcgatcggtg ttgacgacca ggtgatacag
      541 gagcgggtca tcgatgtccc tgtcgtaata cttcgccacg tatcgcttcc gcgcggcatc
      601 tctggtacgg aggtactccg ccgcttccgc aggaccgacg tgaaggtagt cctgcaggtg
      661 ctggatccgt cttgggaccg agcccacgag gcgaacgtgc agcgcgtagg cgagtcgatc
      721 ggtgatgacg ttgccgcccc gaccgatcac gacgacgttg ccgatctcgg cgagccgcaa
      781 gatcgtgtcg gccgtcttgc gaaccagcgt ccacctgggg ggatggaccc ccaggatctc
      841 gtcgatcgta tcggcgatgc cggagacgcg atcctccggc atgaactcgg ccaaccgatc
      901 ggggagcttg tggtcctgga gcaccctctc cacgaggtcg cggtcgaaca tggtccacgg
      961 cggggaggcc tgcggatcgc tggcctggag ccccgcgacg agcgcctccg ccacgacgtg
     1021 cgccccggaa ccagcctggc gggagatggt gatcacgggg cggtgggcgg caggggctgg
     1081 cgactccggt cggcgtcgca gctcgtactt cggtccctcg aggaagcgct ctattccaat
     1141 gatggacttc atcgttgcca ccctttcctg atcgcgagcg accgatcccg tcggcccaac
     1201 cgctcgactg ccgtgacgtc aagcattcac cgcctaccgc aacgataggt cgctgcatct
     1261 gggcaggcct ccggtgaacg tcccggtggc tgggggtcat ccggcccgcg ccgaaccgac
     1321 gtcacgaaga gtgacgtttc cccggcgccg cgatggtgcc tcggatgcga gaggtcctcc
     1381 cccaggggtg accttcggtc ggaggcgttc gggtggtggc gttgcacact agtaagcgga
     1441 ggtgatggcg atgacgctga ccgtgatcgg aggattgctc ttcttggcgc tcatcgtggt
     1501 ctccgtgatc gcgagacgcg agggccacac caaggcctcc tgaactcgga aacgatagtc
     1561 cctccgaggc actgagctcg ccgttgggag aaccccgcta cggccgtggt tgctggcccg
     1621 gcgcacccgg gaaaccggcc tgcacagtgc ggagccagcg cgtcagccga gggaaggtcc
     1681 aatccccctc cgcaaggctg gacctgctat cgcggagatg gtgcgcggct cttccagcgg
     1741 agcccagaac gcgtccggcc gcatggagaa gccagtaccg tccggccttc ggcaagcttg
     1801 tcatggcgtt ggtgccgtga tcttcgcgca gtcggagcag gcctccgcca gcgctccgag
     1861 gccccccgtt ctcgcggttc gtgatcatcc cggcccgcgg ctccgcatca ttgaacgcac
     1921 tcgcccgagg agcggacggg ccgaggtcct ggaagtggtc agggacaccg cgctgacgcc
     1981 acttcgaggt cggtgtgagt gaaccggatc gagccccccg gctcccgcat gctgcccgaa
     2041 ggcagtggcg cctcgcgggt ggcgaccgaa agcctgggcg accaggcagc tcaggccccc
     2101 tcctccaggt gtctcacgat cacgactggg cacgtggcgt attggcgcac tgctggctca
     2161 ccgagccgag aagcagctcc tggaacccgc cgaagccgcg cgagccgacg accagtagat
     2221 tcgcgccctt cgcgacctcc acaagcacct tggcaggttg cccttccgcc gcgatggtct
     2281 ctatctggac ttcgcgagcc tccgcccggg cttcaaccag agctctgcca ggtggccttc
     2341 ggcacccgcg gtcagactct tcgtcatctc ctcggagatg acgaagccgt atccagacac
     2401 atagctgtag gtcgggatct ccaggcagag acgagttcga gcttcattcc ccgcagcttc
     2461 gcctctctcg ccgcccaacg gactgccttc ctcgcggtct cggacccgtc aactcctact
     2521 accactcgcc cgctcatcgg ttcctcctga ccactttgac tcctcttgtc gacttcatct
     2581 tcggattcga acggcagacg ctactgagcc atccggtccc ctctcgacgg gacgttcgtg
     2641 ctactggcgg gcaagcttct cgtccttcag cgggtgcttg ccccgtcgag ccgggtagag
     2701 gacggcacct gtgacggccg aatcgagcag gcactcgcct tcaccgaccg tgcggatcgc
     2761 cccggacgag ctcgccgctc ttgacccatc ttgcccatcc tccggagaag ggactcggct
     2821 gcgagcacgg ccccgcgaca tacatctggg tgcggaagct cgtgcgcgac gggctcagtc
     2881 catgccgctc ctcgcggcca agggcctggc gggcggtcgt ccgatcttcc tggcgaggcg
     2941 agcgaacctc ctcgcattcc caaaggactc gaggtagagc aggctcacgt ctgtagcctc
     3001 gtcgagggac caatgagcca gcagatcgtc gccggagatg tcggccttgt tgcccatcgg
     3061 gacgaacgag gacggtccga ggccgcgctt aatcgcgtgc tgcgtcaccg ccagtgcgag
     3121 cgcaccagac tgcgacagga agccgatccg tccagccggt ggatacacgg tcccgaaggc
     3181 ccattcagtc ggacgtcctc ggcggtgttc acgataccta tgcagttcgg accgatcagc
     3241 cgcatgccga acgaacggta gatctcgagg agcctggtct gccgctgcgc gccctggcca
     3301 ccaacctcct cgaactcaac ggagataagg acgagtccgt ggataccctt ccgaccaaat
     3361 tgctgcgcat ctcctcgacc tctgccgcgg gaacggcgat aaacgcgacg tcgacatcac
     3421 ccggaacgtc gaccatcgtc ggatacggga cgacaccgtg gacccacatc cgccttcggg
     3481 ttgatcggac acacgggacc gtggaactcg atcatgagca gcttatggag gagccgtcct
     3541 ccgattgtgg aagagtctcg ctaggttcaa gctcggccgc aagtgggacg aacgcggcgt
     3601 gctcgtcacg gaacaggtcg cgcgtcctaa cccgtacgga ctcggtccgg gtgccggccg
     3661 cgaacacgac cgtgtcgcac gccactaggg aggggtcgac gagggtctcg gcgtcgagca
     3721 gcaatccgac gaccggcgtg gcgccgagct cgatggacgg gaagtccccc tggagctcgt
     3781 cctccgttgc aagacgggcc gcttggtcgt cgagcgcccc ccgcaccaga tacatgtcca
     3841 gccgccggga agccggtatg acggcgaccg cgtgccgtcc ccgtgtgtcg atcaagacgg
     3901 ccttgaggac gtggtcggcg tccatgtgga ggctgcgggc ctcctcgagc gaggtcgtcg
     3961 acgggtcatg gtggatcacc tcgtaaggga tcccgcgtgc ctccaggtat tcggtgacga
     4021 acgacatggc gaacctcctt gtcgcctcga ggttaggtgg cacacgcggg cgccgaaagg
     4081 gccgaacgtc ctgatcgttc cgggcacgag acccggtggt gcaaggggtc ccggacgcgc
     4141 agggccggtc agccgcgacg cgggatggcc cccgcggcta cggtgatcgt ggcctgggag
     4201 gccctgtgat ccgatcggac gtgtcgatgc cgtcggttcc tctcgtgcag gaggaacccg
     4261 tgcgctggcg gatccagcgt catggggcta tgcgggttcc cggcatcgtc ttcgcgtcga
     4321 agaccctgct cccagacgtg ctctcggatc gatcgcttac tcaggtcgtc aacgtcgccg
     4381 cgctgcccgg catcgtcgag gcctcctatg cgatgccgga catccactgg gggtacgggt
     4441 tcccgatcgg cggcgtagca gccacggatg tggaacgcgg gggtgtcgtg tcccccggtg
     4501 gcgtcgggtt cgacatctcg tgcggtgtca ggctgctcgc ctcgccgttc gaccgcgacg
     4561 agctggcgcc ctccttcgag gccctgatgg acgagctgga cgagacgatc cctcgggggc
     4621 tcggcggcgg aggcgtctgg cgcctccgcg acgaggaact gaacgagatc ctggtcggcg
     4681 ggtcgcgttt cgccgtcgag cagggattcg gcgtcgagcg ggacctcgaa cgctgcgagg
     4741 acggcggtgc ggtcggtggt gcggatcccg gccacgtcag tcagcgtgcc atgcagcgtg
     4801 ggttccggca ggtgggcagc ctcggatccg gcaatcactt cctcgaggtc caggtcgtgg
     4861 aagacgtcca cgacccggca gtggccgcgg cgttcggtct gcgtgccgct caggtctgcg
     4921 tgatgatcca ctgcgggtct cgcggccttg gtcatcagat ctgcaccgac cacgttcgga
     4981 cgatgctcgc agcgatgcct cgatacggga tcgaggttcc cgaccggcag ctggcctgtg
     5041 cgcccgtgga ctcacctgag gggagggcgt acctgggcgc gatggcggcc gcggccaact
     5101 atggccgcgc gaatcgtcat ctcctggcgg aggccgcgcg ggacgccttc gcgagcgcga
     5161 ccggtcgccg cgaactggac ctggtctacg acgtttcgca caatctcgcg aagccagaag
     5221 agcacgtcgt ggacggcgag cgccgtctgc tctgcgtcca ccgcaagggc gcgacccgcg
     5281 cccttccgcc gggcgatcgg gatctcccac cgtccctgcg agcggcgggg cagccggtgc
     5341 tcatcccggg atcgatgggg acggcgtcat acgtgctcac cggcgtcgcc ggtggcggcg
     5401 cgttccactc cacgtgtcac ggagcggggc ggaccatgag ccgtcacgcg gcgaagcgcc
     5461 gcgtccaggg agccacgctc cgcgccgagc tcgaggcccg cgggatcgcg gtccgcgccc
     5521 gatcgatcag agagctgccg gaggaagcgc ccttcgccta caaggacatc gacgaagtgg
     5581 tttcgacctg tgagcaggca ggcctgtgcg cccgggtcgc gcgactgagt cccataggag
     5641 tcatcaaggg ctgactcgga tcacacgtcg attgtggctc ggcaacgcca ccggccctcg
     5701 tcctcacgag tgatgctcag ctcgttgagc gagacacctt tcggcgggga gccgtggatc
     5761 tccaccgccg acaagggtgc aaggtcgatg accccgacga cgcggccgtc ctcggtctgg
     5821 tccagagcca ggtccgcagg gacgcctcct agaacgtcga tcacgtagat gacctcatcg
     5881 agcaacgaca ccaagagggc ctcgtctgca tgctcgtcga actcgatcgg aacgggcttc
     5941 gtggcggcaa ctgcaacgcg tgcgaacgag tcgacgagcg cgcgcaccgc ctcctccagg
     6001 cactccagcc tgctggtccc ccacgcctcg atgatgacat cggccgtgtg gggaagcacg
     6061 cggtgcccgc acgccggcga cggacgctcg ttcatccgct ctcgagatcg gcatgccact
     6121 ggtccatcgc gctctcgttc actcacgcta ctgctcgacc atctgatcgt gcccgatgcg
     6181 gagccgaccc tccgggaagt cccgcgccaa cctgcacgat cccccgggct gtgggcaggg
     6241 ccgttggtgc cccgtttcgt ggccgaacag ccctgaccgc tccccggtga ggcatcggtc
     6301 ccgcgtcggg agaccttgcc actgttacgg agacgcgccg gcgcccgagg tcccgtttcg
     6361 acgggtcgtc cggcggtctc ccgccatgcg ccggtcgacg aacaatcttg atcccctcca
     6421 ttagagacgg ctcgctggat ctcctcgcct tcagccctat acggccgtgc acctcggtca
     6481 ccggaccaac gccccccttt ccacaggtcc cgggagtgat gaggggttcg accgatcggt
     6541 ggctagccgt gcgcgtcggt ctcgcccgcc aggagccggg ccacctcgtc gcgttcgggc
     6601 caggccagcg gccgaccgtc ggcgtcgcag actcctgagc cgtccccggc ccgaccgatg
     6661 tccgaagccg tgtacccgtg ccgagccagc gcccgcatgg ccggccccgc tcgatcgggc
     6721 gggaaggcgg ccagtagggc gcccgaggcg agcgttgcca tcgggtcggc ggccagcgca
     6781 tcgcagatcg cccgcccggg cgagaaccac agcaccgagg acagatccac ccggatgcgg
     6841 acgcccgagg cttccgccat ctcgtggagc ccggctgcga gcccgccttc ggtcgggtcg
     6901 tgcatcgcgc tggctccgag ttcggcagca aggagcgcgg gttcgaccac ggagatgccc
     6961 ggatggtccg gggccaccag cgccttatcg aggtcgccgc gatccagccc ggtcaggcgg
     7021 tcggccgctt cgcgggccag gacccctgca ccctcgatcg gtgcggggcc aacttggagg
     7081 agcacgtccc gcggccgcat cccgcccgtc gtaacgaact tcccgtcctc ggcgatcccg
     7141 aggaactggc cgacgacgag cggctgcgtg accgccggcg tcacctcggt atgaccgccg
     7201 accagggagg cgccgacgtg agccaggccg tcccgcatgt cggcgaagac gtcacgaacc
     7261 atcgcctcgg tacttccgga aggaagcagg atgacagcca ggaaccaacg cggccgaaca
     7321 cccatcacgg ccacgtcgtt ggcgttcacg atgaccgaga agcgcccgat ctcgcgcgcg
     7381 gtgaaggtga tggggtccgt tgccgctacg aggaccccgc cgggaacctg gatggcgcac
     7441 gcgtcctcac cgatgtgagg ccccagcagc acgtcggccg gcggaaccgg aagatcagcc
     7501 agcagatcgg caaggagcct cgccggcagc ttgcctacgc gaagcggtgc tcgcggaccc
     7561 gagctgcctt ccaccgctgc agcctagaag gtcatctcac tccttggaac tggtccgtgc
     7621 cccccgtgcc gtgggtcctc gagcgaaggg gtccccaggc ctctactggt cgtatgccgg
     7681 ccgcttaggt tcggattcga aaggagccgt catgacgatg catggacacg gatctatcgt
     7741 gcaggcgcat ccgcacccgc acgcggtcca ttaccagcgg cacgggacga cctgggagca
     7801 tctcaccaca cccatggtca cgagcacgac cacccgccgt tgagcagccg tctgtaatga
     7861 atacccggcg gttggggggg tcgtgatcgg accatctcgg tcgtcaacag tcagttggtc
     7921 gcatccaacg tgggttgctc tacggggact ggcggatgac gccggggcgg cttcacacga
     7981 ccctagtcga gcctacgtgc tcgcgcgacc gggcgggctt ttcggcgatc cccgttgatt
     8041 gatataagca gctgccaagc gcccctcgcc ggagaggaga cctcgatgcg cggtggagga
     8101 tcgaccacgt gcaacatgcc cgaggtcgct ggcgagctcg aagaggcccc cgcgaaccgc
     8161 gccatcggaa cgacgcggtg gttcgagacg agcatgttcg ggtctgggag gtccgcctcg
     8221 agcctgcaca gcgtgcccgt tccacgggca cgctcagacc tactgctgga cctgcatcgt
     8281 gatcgaaccg agcgagatcc agcggatcgt cgagacactc ggtcgggccc tgaccgagtg
     8341 tgtggcagac cccaccaccg ggcggcggac ctgatgcgcc tgatggcggc cggtcgctgc
     8401 cactggcgaa ccccgatccc gatcacgtac cagaccgacc tggggaaggg ctatgcgcag
     8461 ttcgagatct tggccctcta cttcagcgac gtgaagatcg tcgacgtcag cggtcgccgt
     8521 gccgactaac cgcccgaagg ggttcgccta cg
//
LOCUS       DASUYD010000166         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1640936, whole genome shotgun sequence.
ACCESSION   DASUYD010000166 DASUYD010000000
VERSION     DASUYD010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1640936"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF195_09555"
     CDS             <1..280
                     /locus_tag="VF195_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581102.1"
                     /translation="VEKKGFMASMMERAEQQRQQRGSATKPVAKKPGSSPKPGSSPKP
                     GSNPKPGSNPKPGSNPKPGSNPKPGSNPKQSGSGGNGSGGDPKKRPKR"
     gene            246..914
                     /gene="jag"
                     /locus_tag="VF195_09560"
     CDS             246..914
                     /gene="jag"
                     /locus_tag="VF195_09560"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HEX6581103.1"
                     /translation="MEEIRRSAPSVEEAVEAALGELGLTEQEVDVDVLQEPRPGVFGV
                     GAQDAIVVVRSRAKPAGDLGEEDLDEQGEIAAEFMEGMLERMGITATVELAIEDGTMY
                     VDVIGPEPDDEDMGLLIGRHGQTLEAIQELTRVVIGQRTGLPSRVVVDIEDYRKRQIE
                     RLSARVREIAVRVSRTGRAETLEPMNPFLRKMVHTAVAEVDGVDSSSEGEGAERRVVI
                     SRAR"
     gene            963..1571
                     /locus_tag="VF195_09565"
     CDS             963..1571
                     /locus_tag="VF195_09565"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX6581104.1"
                     /translation="MSEAEILLGQAAELGVTLYASQAATLLRYEDLLSRRAVPLGAVA
                     HSDATRIRDRHILDCLRAAPLVHEDDVACDLGSGAGLPGIVVAVAEPLVRVLLVESRA
                     KRAALLEFAVQELRLSNAQVVVGRAEELRRGVDACFARAFAPLPLAWSVARGILRPGG
                     RLVYFAGAELGDPAVPDGAALEAVVRTPVLESAGPLVIMTRQ"
     gene            1664..>2587
                     /locus_tag="VF195_09570"
     CDS             1664..>2587
                     /locus_tag="VF195_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016699007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX6581105.1"
                     /translation="MQRSSKASRNAPETSGSAPAASRPARIIAVANQKGGVGKSTTAV
                     SLGASLAELGHRVLVVDLDPQGNASTGLGIRHEQREVTVYDVLADEAPIEQAIVPTPI
                     DNLFAIPSTIDLAGAEIELVSQFSRELRLKKAIEPLKHGAYDYVFLDCPPSLGLLTVN
                     ALAAAEELIVPIQCEYYALEGLGQLLRNVRLVQQNVNTGLRLTGIVMTMYDPRTKLSE
                     QVVAEVRRYFGDRVYQTVIPRTVRLSEAPGFGQPINVYDPTSKGARSYRDLAREVAAR
                     LPDERPLPNVDDVPSVTIPRPEQPPSRAPRPL"
BASE COUNT          444 a          861 c          892 g          390 t
ORIGIN      
        1 ggtcgagaag aagggcttca tggcttcgat gatggagcgc gcggagcaac agcgccagca
       61 acggggctcg gcgaccaagc ccgttgccaa gaagccaggg tccagcccca agccggggtc
      121 cagccccaag ccggggtcaa accccaagcc ggggtcaaat cccaagccgg ggtcaaaccc
      181 caagccgggg tccaacccca agccggggtc gaatccgaag cagtcagggt ccggaggcaa
      241 cggcagtgga ggagatccga agaagcgccc caagcgttga ggaggccgtc gaggccgccc
      301 tcggcgagct cggcctgacc gagcaggagg tcgacgtcga cgtccttcag gagcctcgtc
      361 ccggggtctt cggggtcgga gcgcaggacg ccatcgtagt cgtccgctcg cgagcgaaac
      421 ccgcggggga cctcggcgaa gaagatctgg acgagcaggg cgagatcgcc gcggagttca
      481 tggagggcat gctcgagcgg atgggcatca cggcgacggt cgaactggcg atcgaggacg
      541 ggacgatgta cgtggacgtg atcggtcccg aacccgatga cgaagacatg gggctcctca
      601 tcggtcgaca cgggcagacg ctcgaggcga tccaggagct gaccagggtc gtgatcggcc
      661 aacgcacggg cctcccctcc cgggtagtgg tcgacatcga ggactaccgg aagcgacaga
      721 tcgaacggct ttcggcacgt gtgcgcgaga tcgccgtgcg cgtctcccgg acggggagag
      781 cggagacgct cgagccgatg aacccgttcc tgcggaagat ggtccatacg gccgtggccg
      841 aggtcgatgg ggtcgacagc tcgagcgagg gcgaaggagc cgagcgacgc gtcgtcatca
      901 gccgagcgag gtagccgcac ggagtgaccg gccgtcaagg tgtcgtttca cgtgaaacat
      961 cgatgagcga agcggagatc ctgctggggc aagccgcgga gctcggagtc acgctttacg
     1021 cctcgcaggc cgctaccctc ctccggtacg aggatctcct gtcccgtcga gcggtcccgc
     1081 tcggtgccgt cgcgcattcg gacgctactc ggatccggga tcgccacatc ctcgattgcc
     1141 tccgcgcagc gccgctggtg cacgaggacg atgtcgcctg cgatctcggg tccggagcag
     1201 ggctgccggg gatcgtcgtc gccgtcgcgg agcctttggt ccgggtcctg ctcgtcgaga
     1261 gcagggcgaa gcgagccgcc ctcctcgagt tcgccgtcca ggagctgcga ctgtcgaacg
     1321 ctcaggtggt ggtgggacga gccgaggagt tgcgtcgggg ggtcgatgcc tgcttcgccc
     1381 gggcgttcgc cccccttccg ctggcctggt cggtggctcg gggcatcctc cgcccggggg
     1441 gtcgcctcgt ctacttcgcc ggcgccgagc ttggggaccc cgcggtcccc gacggggccg
     1501 cgctggaagc ggtcgtacgc acgcccgtgc ttgaaagcgc tggtcccctg gttatcatga
     1561 ctcgtcagtg acgaagccct cctcttcccg ggcagctcgt tcgtcctcca agcccaagcc
     1621 agtcaaacga gcaagcgagg cttcggggac gcaaccgact gccgtgcaac gctcttcgaa
     1681 ggcgtcccgc aacgcgcccg agacttcggg ttccgctccc gcggcctcta gaccggcgcg
     1741 gatcatcgcg gtcgcgaacc agaagggcgg tgtcggcaag agcacgacgg ccgtgagttt
     1801 gggagcgtcg ctcgcggagc tcggccaccg ggtcttggtc gtcgatctgg acccgcaggg
     1861 gaacgcctcc acggggctcg gcatccggca cgagcagcgc gaggtcaccg tgtacgacgt
     1921 gctcgccgac gaggctccga tcgagcaggc gatcgtcccc acgccgatcg acaacctttt
     1981 cgcgatcccc tcgaccatcg accttgcggg cgctgagatc gagctggtga gccagttctc
     2041 acgtgagctc cggctgaaga aggccatcga gcccctgaag cacggcgcct acgactacgt
     2101 cttcctcgat tgtccgccgt cactcgggct cctcacggtg aacgccctcg ccgccgcgga
     2161 agagctcatc gtgccgatcc agtgcgagta ctacgccctc gaaggtctgg gacagctcct
     2221 gcgcaacgtc cgcctcgtgc agcagaacgt caacacgggc ctgcgcctga ccggcatcgt
     2281 gatgacgatg tacgacccga gaaccaagct gtccgagcag gtggttgccg aggtccggcg
     2341 ctacttcggc gatcgcgtct atcagacggt catcccgcgt accgtcaggc tttccgaggc
     2401 gccgggattc gggcagccga tcaacgtgta cgacccgacc tcgaagggcg ccaggagcta
     2461 tcgcgatctc gcccgggagg tggctgcacg cctccccgac gagcgaccgc tcccgaacgt
     2521 ggacgacgtg ccgagcgtca cgatccctcg ccccgagcag ccaccgagcc gggcgccacg
     2581 tccgttg
//
LOCUS       DASUYD010000167        11269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1641908, whole genome shotgun sequence.
ACCESSION   DASUYD010000167 DASUYD010000000
VERSION     DASUYD010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11269
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1641908"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="VF195_09575"
     CDS             complement(<1..334)
                     /locus_tag="VF195_09575"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581106.1"
                     /translation="MSKARRASEPVEDPIRVLVADDEETVVDVLRALIGSDPSLRFVG
                     AAHTAEDAIEMTARERPDVVLLDVRMPGGGGLRATREITKRCAPTKVVALSAHQDSDT
                     VIGMISAGA"
     gene            825..1478
                     /locus_tag="VF195_09580"
     CDS             825..1478
                     /locus_tag="VF195_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581107.1"
                     /translation="MKWVGRSTLVVMSTMAIVATGAATAQAACPVLDPLCVVEEAVDD
                     GVGLVDDTVGGIETPVDDVLDPLEEVVDPVVDTIFGTVDDVIGGGGGEQPEPPEGIGS
                     DDGAQPGVHQGGPGPSGPPGRETVDIVGPRVHERPEIGGVFAPVVAPRPDADATGSPM
                     EGSGRVGVAIGAVARSLAIVLALFGLTLGFAAIQDRLDRNDPRLSLAPIRSDLVTFA"
     gene            1505..3358
                     /locus_tag="VF195_09585"
     CDS             1505..3358
                     /locus_tag="VF195_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581108.1"
                     /translation="MPTQAVELLSLTERSRYLSGLRLGFAGILIATTLASDDVASGQV
                     SVAVVLYLACSALGAGIVGRGGRAAGPTLNGSLLVDGIFLAIVIARTGGTAEALLLLP
                     GVHIVGVTLLLSYQTGLKIALWHTLLFLLVVEAARVGFVQGQVGAALGRGAGLVVVGL
                     WALALGTAFFSAVSERELRRQKHDLFRLSVMVAELDTEPDVHAIAQTFLDELCSTFGF
                     SRGVILASPGGDLEVLAWIGEGSSTDVSVGVDRTVSRAWAERTSQLVRSIDPETDPRI
                     ASLIPGARNAVIVPLLRDRRQGLGAVIVERGDDHPTERRWVIEMMEQFAEHGALALHN
                     AWLTEERESQLRVIQGLEQELRAHNVTLEATVAKRTRELQGLVRELQEIGEQRKQLLE
                     HVVRAAEDERTRISHDIHDDPVQKMVALKMRLELLRRSHPEPAEMDEALAVMRVAIKS
                     MRTLLFDLSPPTLEEQGLASALRFMLEQSAWPFEWSVDEGADDDFPIWSSLILYRIAQ
                     EALTNARKHSGAERVSVSLERREGGTWMQIADDGVGFRPQDALVAAPGHLGLAAMKER
                     AEMAGGWCKLRSLPDEGTTLEVWLPDVETPHPAPSLGDGTVTELLPLPGRL"
     gene            3627..3848
                     /locus_tag="VF195_09590"
     CDS             3627..3848
                     /locus_tag="VF195_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581109.1"
                     /translation="MGETFHVVPVDDWCGHEEVQDSCVCGPHAEFIAGGKVIVHHALD
                     GRHADDPSWPRRRASEVAIEIFLAEEEIA"
     gene            complement(3910..4707)
                     /locus_tag="VF195_09595"
     CDS             complement(3910..4707)
                     /locus_tag="VF195_09595"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581110.1"
                     /translation="MGWIAAIDWEGGLEDAWDAIIVFVPKLLGFLLILGIGYFVAKAI
                     EKVLDKLLERVGFDRIVERGGVKRALSRSRLDASSILSRIVFYIAFLFVLQLAFSVFD
                     PNPITDLLEGVIAFLPKLFVAVIIVVITAAIATGAKTIIESMLGGMSYGPMLATIAAV
                     AIWFIGIAAALNQIEVAPAIVNGLFYAVLALVVGVGIVAIGGGGISPMRERWQRALTR
                     LDQEGPRLKQQAAGGSEVVREKMDEVRREVGDARTEQTSSTTETRSL"
     gene            complement(4878..5603)
                     /locus_tag="VF195_09600"
     CDS             complement(4878..5603)
                     /locus_tag="VF195_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581111.1"
                     /translation="MRDAEGSAFVPRDNSLWLADDNGRAIYEVNPVTGTLKRRISASS
                     FESANRFGGGATAGADRVRDFESLAYDEANDILYVFSGPCCTSSVLPTAFRLLRGGGG
                     TLQVDSYQPLGSGANYTGAGWNSADGKTYVGVGAKLRSYDFVTNTAGPIFKVPNLSGI
                     TGMAFSSTDLYVTTNAEKLRRVDWASKTLVAGWTFDLTPFQIRDSRAVDLIDDRFYVL
                     DGYDGRSSGDPLRHAVFVFDVLP"
     gene            complement(5890..7407)
                     /locus_tag="VF195_09605"
     CDS             complement(5890..7407)
                     /locus_tag="VF195_09605"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6581112.1"
                     /translation="MGGTSRVSRSALKRLAWAGMVLSLTAAFGLPTAAFAAASTVTLV
                     GDPGTVTVTADDLANGINLNDSAGYVTVVDTIQGVTPGAGCEVDPNVVGGVRCLKPAG
                     GVSIIDVILGAGNDGLLSAGLDEPLHVLGGDGNDTIDGGSQADVLEGDAGDDTLDGKG
                     LDDVLTGGADIDTADYDGAPDRIVSLDGVANDGAVGENDNVDTENVTTANGNDTVLGD
                     ANANRIVSGAGNDTLTGGPGGDILNGGADVDTVDYSSAGGSVVVTIGGGANDGEAGEL
                     DNVSISVENITGGPGDDTLTGSGVANRLLGGVDPDPTLNDGDTGNDTLIGGLGDDALS
                     GLDGNDELFGEDGADTLNGGVGADILDGGLGSGDSVGYPGRTSGVSVNQNKPGGDGQG
                     GSAEGDDVRASVERVTGTAFADTLVGGKVNASILIGGGGNDVLNGGNGHDDIVNGGAG
                     NDRINDNGGRDSVLGSAGKDTFHTSDGARDLINCGKGSDTSSDRDPIDVRKASCE"
     gene            7715..8035
                     /locus_tag="VF195_09610"
     CDS             7715..8035
                     /locus_tag="VF195_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581113.1"
                     /translation="MRRWVIAGIGSIVITAAVGSVIASANSDSEGGVTGRQAERAIHA
                     ALEATGGGIANAVERDSENGAYWEVEISTPDGRVGDVRLSRDYSVVVIEGDSEAADTG
                     DGGS"
     gene            8159..9067
                     /locus_tag="VF195_09615"
     CDS             8159..9067
                     /locus_tag="VF195_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581114.1"
                     /translation="MRRTTRAFMLGALTCAILLPALPAHAVWEGRVVAQDSLKYPNTP
                     IPDPTGIAYNGKKGTFFISDAEVDETPSLWHKRNLFIVGRGGNLKAARRLRMTTEPEG
                     IAWWGAKGILFVADDDQDLILTYRKGRDGAIGTGDDKVRKLINTRRWGSLDPEGLTFS
                     PKGAVLIWVDASNQKVYKLRRGNDLRFGTADDKIAKFGTAKFGFTEAESVAVHPVSNH
                     IFVVNSNVNEIVETTFKGALVKRIVIPSSECPPACNLTGLVFAPGTDGSRRRLYLTDK
                     GVDGDFNPDPVQVNDGQLYQMRLVNV"
     gene            complement(9693..9938)
                     /locus_tag="VF195_09620"
     CDS             complement(9693..9938)
                     /locus_tag="VF195_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581115.1"
                     /translation="MAIIAFMVILVMAAAARPGRGWRLGATLAAAAAGGVGVASLVAG
                     SAASTVPKVGAVAAIAWGLAGLWLSVRHGVAARNIPG"
     gene            complement(9914..10546)
                     /locus_tag="VF195_09625"
     CDS             complement(9914..10546)
                     /locus_tag="VF195_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581116.1"
                     /translation="MERLEQPLSRGRTRAVRIVAVLGLLLFGSFFSIVALNTLGVVSS
                     GAEGSPDDPPPSAEARFGAAHPGEALHAVGAVAVVVIGASGLVAMIVRPERPGHAYQV
                     LAAMTGVLVTLSIVGDPDNVGGQASPIDPSLLLVTVPAVVAALLAAPWRRRGSDDRWR
                     THLLALAAVGAIPAARYGIDQALIQRNTFPPTADPHHTLTGGPWRSSPSW"
BASE COUNT         1931 a         3763 c         3735 g         1840 t
ORIGIN      
        1 tcgcccccgc gctgatcatg ccgatcacgg tgtcggagtc ctggtgcgcc gagagcgcga
       61 cgaccttcgt cggcgcgcat cgcttcgtga tctcgcgggt cgcccgcagg cctccaccgc
      121 ccggcatccg cacgtcgagc aggaccacgt ccgggcgttc gcgcgccgtc atctcgatcg
      181 cgtcttcggc ggtgtgggct gcccccacga agcgaaggga cggatccgat ccgatgagcg
      241 cacggaggac gtcgacgacc gtctcctcgt catcggcgac caggacacgg atcggatcct
      301 cgaccggctc cgaggcacgc cgcgctttgc tcacttgctc tccttccgtc cggcgacccc
      361 gaagcgtttc tgaggccgga tctgaagctc ccacatctac ccggaacgag gtatcggtat
      421 gcgcgtgacc tgggaggatg gttccttcgc gtcaatctca cgtggctcgc cctgaccaga
      481 cgtcccgtgc atccaaccct cactcgtccg accgacaggc gcgcatcggt cggggggcga
      541 aagaccatcc ccgggcgcgc cggcgcgcac gaacgacaca ccgatcacgt atctccgcac
      601 acgttcgtcg atgggccgag aaggggaccg gctccggtcg gcgccctcgg gcggcctcgg
      661 tgccctactg cgatggatcg gcgatgcggc tcggggatga catgagatga accccgatgg
      721 ctattggcta cggacgtact ggggccgata tagagggaga ccgaaaggaa cgccgtgaca
      781 gaggcaggtt ccatcgcagc aggtcgaccc tcggaggtga caccgtgaag tgggtcggtc
      841 gttcgacctt ggtcgtgatg tcgaccatgg cgatcgtcgc tacgggggcc gcgacggcgc
      901 aggcagcgtg tccggtcttg gacccgctgt gcgtggtcga ggaagccgtg gatgacggtg
      961 tgggcctggt ggacgacacg gtgggtggta tcgagacccc cgtggacgac gtgctcgatc
     1021 ctctcgaaga ggtcgtggac ccggtcgtcg acacgatctt cggcacggtt gacgacgtga
     1081 taggtggggg cgggggagag cagcccgaac cgccggaggg catcggctcc gatgatggag
     1141 ctcagcccgg ggtgcaccag ggcgggccgg gaccttcggg acccccgggt cgagagacgg
     1201 tggacatcgt gggtccgcga gtccacgaga gaccggagat cggcggggtc ttcgcaccgg
     1261 tcgtggcgcc gagaccggac gccgacgcga ccggatctcc catggaggga agcggacgtg
     1321 tgggagtcgc catcggcgcc gtcgctcgaa gcctggccat cgtgctcgcg ctgttcggat
     1381 tgaccctcgg gttcgctgcg atccaggatc ggctcgatcg caacgatccg cggctctccc
     1441 tggccccgat cagatccgat ctggtgacgt tcgcgtgatc gcccttcctc gaacccgtcg
     1501 gaggatgccc acccaggcgg tggagctgct gtcgctcacg gagcggagcc gctacctctc
     1561 gggactccgc ctcggcttcg ccggcatcct catcgcgacc accctcgcct ccgacgacgt
     1621 ggcgagtgga caggtctccg tggccgtggt gctctatctg gcgtgctccg ccctgggcgc
     1681 agggatcgtc ggacggggcg gccgcgccgc cggcccgacc ctcaacgggt cgttgctcgt
     1741 ggacgggatc ttcctggcga tcgtgatcgc ccggacgggc gggaccgcgg aggcgctcct
     1801 cctcctgccc ggagtgcaca tcgtcggcgt cacgctgctg ctctcgtatc agaccgggct
     1861 gaagatcgcc ctctggcaca ccttgctgtt cctcctcgtc gtcgaagccg ctcgcgtcgg
     1921 cttcgtccag ggacaggtgg gggcggccct gggacgaggg gcagggctcg tggtcgtggg
     1981 cctctgggcc ctcgccctcg gcacggcgtt cttctcggcc gtcagcgagc gggagctccg
     2041 gcgacagaag cacgacctct tcaggctgtc cgtgatggtc gccgagctgg acaccgagcc
     2101 cgatgtccac gccatcgcgc agaccttcct ggacgagctc tgttccacgt tcggcttctc
     2161 gcgcggcgtg atcctcgcgt cgcccggcgg cgacctcgag gtcctcgcct ggatcggtga
     2221 aggctcgtcc accgacgtct ccgtcggcgt cgatcggacc gtgtctcggg cgtgggcgga
     2281 gcgcacgagt cagctggtga gatcgatcga cccggagacg gaccctcgca tcgcatcgct
     2341 gatccccggt gctcggaacg cggtgatcgt cccgctcctc cgtgaccgcc gccaaggact
     2401 gggcgccgtg atcgtggagc gtggcgacga ccatcccacc gagcgccgat gggtgatcga
     2461 gatgatggaa cagttcgccg aacacggagc gctcgcgttg cacaacgcct ggctcaccga
     2521 ggaacgggaa tcccagctcc gggtcatcca ggggctcgag caagagctgc gggcacacaa
     2581 cgtcaccctc gaggccacgg tggcgaagcg aacccgcgag ctgcaaggct tggtcaggga
     2641 actgcaggag atcggcgagc aacggaagca gctcctcgaa cacgtggttc gggcagccga
     2701 ggacgagcgc acccggatct cccacgacat ccacgacgac cccgtccaga agatggtggc
     2761 gctcaagatg cggctcgagc tgctccgcag gagtcatccc gagccggcgg agatggatga
     2821 ggcgctcgcc gtcatgcggg tcgcgatcaa gagcatgcgc acgctcctct tcgatctcag
     2881 cccgccgacg ctcgaggagc agggactggc gtcggcgctt cgcttcatgc tcgagcagtc
     2941 agcctggccg ttcgagtggt cggtcgacga gggcgcagat gacgacttcc cgatctggag
     3001 ctccctcatc ctgtaccgca tcgcgcagga ggcactgacg aatgcgcgca agcactcggg
     3061 tgcggagcgc gtctctgtga gcctggagcg gcgagaaggc ggcacgtgga tgcagatcgc
     3121 cgacgacggg gtcgggttca ggccacagga tgccttggtg gcggcacctg gccatctcgg
     3181 cctggccgcg atgaaagagc gggctgagat ggcggggggt tggtgcaagc ttcggagcct
     3241 cccggacgag ggaaccacgc tcgaggtctg gctcccggac gtggagacac cgcatcccgc
     3301 cccttcgctc ggtgacggga ccgtcaccga actcctgccg ctgcccggca ggctctgagc
     3361 cgcgctactc tcgctggaag gcgagtcgga ggccgagtgc tatcaggcca cgtgttccgg
     3421 gccaggagga tcatgtcagg gttccgggtc acgaccgcga gcgtggcgac cgcgatgaac
     3481 cccgcgagat ccaccatgcg agggtagcgt gactcgacgg tggcgaggtc gcccttcctg
     3541 gaaccacgac gacggatccg atgcccttcg ccgtgatccg ggagcccggc tacaggagat
     3601 ccccttccgg tccgatgcga tgatgcatgg gcgaaacctt ccatgtcgtt ccggtcgacg
     3661 attggtgcgg ccatgaagag gtccaggact cgtgcgtctg cggtccgcac gccgagttca
     3721 tcgctggggg caaggtgatc gtccatcacg cgctcgacgg ccgtcatgcg gacgacccct
     3781 cgtggccccg ccgccgagcc tccgaagtcg cgatagagat cttcctggcg gaagaggaga
     3841 tcgcgtgacc ggaccgcagc ggaggaaggg ggcgggctga gagcccgccc cccacggcaa
     3901 cgtcttgcgt cagagcgaac gggtttcggt ggttgagctc gtctgctcag tgcgagcatc
     3961 gccgacctct cgtcgcacct catccatctt ctctcgaacc acttcggacc cgcccgcagc
     4021 ctgctgcttc aggcgtggcc cttcttgatc cagccgggtc agcgcgcgct gccaccgctc
     4081 gcgcatagga gagatcccac ctccgccgat cgcgacgatg cccacgccca ccaccagggc
     4141 gagcaccgcg tagaacaacc cgttcacgat cgcaggtgcc acttcgatct gattcaatgc
     4201 cgccgcgatc ccgatgaacc agatcgcgac cgctgcgatc gtggccagca tcgggccata
     4261 gctcatgccg ccgagcatcg actcgatgat cgtcttcgct cccgttgcga tcgcagccgt
     4321 gatcaccacg atgatgaccg ctacgaacag cttggggagg aaggcgatga cgccctcgag
     4381 gagatccgtg atggggttcg gatcgaagac actgaacgcg agttggagaa cgaagaggaa
     4441 cgcgatgtag aacacgatcc tcgacaggat cgagctcgcg tccagtcgcg accgcgagag
     4501 cgcgcgcttc acaccgcctc gctcgacgat gcgatcgaat cccactcgct ccaggagctt
     4561 gtccaagacc ttctcgatcg ccttggcgac gaagtacccg atccccaaga tcaggaggaa
     4621 ccccaggagc ttcggaacga agacgatgat cgcgtcccac gcatcctcca ggcctccttc
     4681 ccaatcgatc gctgctatcc agcccatgat ggtctccttc cagatcgtga cggtcagtag
     4741 cctctcgata cccgcacgtc gcgtaccgca atcgggatgt gaagaactgg gggctctggc
     4801 cccgaacgga tggaacgaga ccgcttggcg cccgccctac acgtccctcg accacgttca
     4861 gcggtcgagc gcgaggttca ggggaggacg tcgaagacga aaacggcgtg cctgagcggg
     4921 tcgccgctcg agcgaccgtc gtagccgtcg agtacgtaga aacgatcgtc gatcaggtca
     4981 accgcccggc tgtctctgat ctggaacgga gtgaggtcga aggtccaccc cgcgacgagg
     5041 gtcttgcttg cccagtcgac acgacgcagc ttctccgcgt tcgtcgtcac gtacaggtca
     5101 gtggaggaga aggccatgcc cgtgatcccc gagaggttcg gcaccttgaa gatcgggccc
     5161 gcggtattcg tcacgaagtc gtaggagcgc agcttcgcgc cgacacccac gtaggtcttc
     5221 ccatcagcgc tgttccatcc agcgcccgtg tagttcgccc cacttcccag aggttggtac
     5281 gagtccacct ggagggtccc gccgccaccg cgcaggaggc ggaaggccgt cgggaggacg
     5341 gaggaggtgc agcaaggacc cgagaagacg tagaggatgt cgttcgcttc gtcgtaggcg
     5401 agactctcga agtcccgaac gcgatcggca cctgcagtcg ctccacctcc gaaccggttg
     5461 gctgattcga aggagctggc gctgatcctc cgcttcaggg ttcccgtgac cgggttcact
     5521 tcgtagatcg cgcggccgtt gtcgtcggcc agccagaggg agttgtccct gggaacgaag
     5581 gcgctccctt ccgcgtctcg catcgagatg gatgacccca cgaaggggga ggttcggatc
     5641 gtcctctcga gttggagctc cggatcggac gaggtgggaa gggacgcgag tgccgggccg
     5701 gctgagaaca acaggagccc gagaagcggc aacagcagtc cacgctgcca cgaacagacg
     5761 cgcccccgcc gcgagctcac ccggacaccc cgactctccc gtgatcccac tttcaggcgc
     5821 tacccgagac gttccgaatg caaacgccac tcgaccgctg gtcgtgcggc ggcacacgac
     5881 cagggaagat cattcgcagc tcgccttccg aacatcgatc gggtcgcgat cggacgaagt
     5941 atcggacccc ttgccgcagt tgatgagatc ccttgcgccg tcgctcgtgt ggaaggtgtc
     6001 tttcccggct gagcccagga cgctgtcgcg gccgccgttg tcgttgatcc tgtcgttgcc
     6061 ggccccgcca ttgacgatgt catcgtggcc gttccctccg ttcaggacat cgttgccgcc
     6121 gcccccgatc aggatgctgg cattgacctt cccaccgacc aaggtgtcgg cgaacgcggt
     6181 gccggtcacg cgttcgacgc tcgcccgcac gtcatcgcct tccgcgctcc cgccttgccc
     6241 gtctccgcca ggcttgttct ggttgacgct gaccccactg gttcggcccg ggtagccgac
     6301 gctgtcaccc gagccgagtc cgccgtcgag gatgtcggct ccgacccctc cgttcaaggt
     6361 gtccgcgccg tcttcgccga acaactcgtc gttcccatcg agcccgctca gcgcgtcgtc
     6421 cccgagccca ccgatcagcg tgtcgttgcc cgtgtcgccg tcgttcagcg tcgggtccgg
     6481 atccacaccc ccgagcaagc ggttggcgac gcccgatccg gtgagggtgt catcgcccgg
     6541 tcctccggtg atgttctcga cggagatgga cacgttgtcg agctcgcccg cctcgccgtc
     6601 gttggcgcct ccaccgatcg tgacgaccac cgagccccct gcgctggaat agtccaccgt
     6661 gtcgacatcc gcgccaccgt tcaggatgtc cccgccgggg ccaccggtca gcgtgtcatt
     6721 gccggcgcca ctgacgatcc tgttcgcgtt cgcgtcgccc agcacggtgt cattgccgtt
     6781 tgccgtggtg acgttctccg tgtcgacgtt gtcgttctcg ccgacggcac cgtcgttggc
     6841 gactccgtcc aatgagacga tccgatccgg ggcgccgtca taatcggcgg tgtcgatgtc
     6901 tgccccgccg gtgaggacgt catccagacc cttgccgtcc agggtgtcgt ctcccgcatc
     6961 gccctccagc acgtcggcct gagatccccc gtcgatcgtg tcgttcccgt caccaccgag
     7021 gacgtggagg ggctcatcca gtcccgcgga caggagaccg tcgttccccg cgccgaggat
     7081 gacgtcgatg atcgagaccc ctccggctgg tttcaggcac cgaaccccac ccacgacgtt
     7141 cgggtcgacc tcgcatcctg cgcccggggt gacgccctgg atcgtgtcga cgaccgtcac
     7201 gtagccggcg gagtcgttca ggttgatgcc gttcgcgaga tcgtccgcgg tcaccgtgac
     7261 cgtccccgga tccccgacga gcgtcaccgt tgacgcggcg gcgaaggccg ccgtcggcaa
     7321 accgaatgcg gccgtcagcg agagcaccat ccccgcccat gcgagccgct tcagcgcaga
     7381 tctggaaacg cgtgaggtac cacccatcga ccaagcctcc cagagagaga tccaacggcg
     7441 agacggcccc accgtcgaga acaccgacac ccagcgggtg cgtgcctcct tgctaccacg
     7501 tgcgctcgct ccgtgagaac gggcgaacgg cccgaaatgc ccaccacaag cccaagaaga
     7561 cgacactggt gagcaccgtt ccgagcgtac aagcccgcgc gcttcttggc cggtgttctt
     7621 cccgggttcc ccgaggaccg catccatcgc gttcaggctg gtttcatctt cgcgggatta
     7681 cgttcgctcc atgccaccgg aaaggaggcc caccgtgcgg agatgggtca tcgcaggcat
     7741 cgggtcgatc gtcatcaccg cggcggttgg gagtgtgatc gcgtccgcga acagcgactc
     7801 cgaaggcggg gtcaccggac gacaggcgga acgagcgatc cacgccgccc tcgaggcgac
     7861 gggaggtgga atcgccaacg cagtcgagcg cgactcggag aacggggcct actgggaagt
     7921 ggagatcagc acccccgatg ggcgtgtggg ggacgttcgc ctgagtcgtg actactccgt
     7981 ggtcgtcatc gaaggggata gcgaggcagc ggatacgggc gacggcggct cgtgatcacg
     8041 gccggagatg agctccgcga aacaaactcc ggaccggagg cggctccgca ggccgggtga
     8101 gacttgctac cgcgaacgcg tggggtcaga ggtgcccgag cgtcggtagg atgccgggat
     8161 gagacgaacg actcgtgcct tcatgttggg cgcactgacg tgcgcgatcc ttctgcccgc
     8221 actgcccgcg cacgctgtct gggagggacg tgtcgtggcg caggacagcc tcaagtatcc
     8281 gaacacgccg atcccggatc ccacgggcat cgcgtacaac ggcaagaagg gcacgttctt
     8341 catctccgac gcggaggtgg acgagactcc ctcgctctgg cacaaacgga atctcttcat
     8401 cgtcggcagg ggaggcaacc tcaaggcggc gagaaggctg aggatgacga ccgagcccga
     8461 ggggatcgcc tggtggggag ccaagggcat cttgttcgtg gccgacgacg accaggacct
     8521 catactcacg tacaggaagg gccgggacgg tgcgatcggt accggcgacg acaaggttcg
     8581 gaagctgatc aacacccggc gctggggttc gctggatccc gagggactca cgttctcccc
     8641 caagggagcc gtgctgatct gggtggacgc cagcaaccag aaggtctaca agctgcgtcg
     8701 gggcaatgac ctccgcttcg gcaccgctga cgacaagatc gcgaagttcg gaacggcgaa
     8761 gttcggcttc accgaagccg agagcgtggc ggtccaccct gtgtcgaacc acatattcgt
     8821 cgtgaacagc aacgtgaacg agatcgtcga gaccaccttc aagggtgccc tcgtgaagag
     8881 gatcgtcata ccgagcagcg agtgcccccc ggcctgcaac ctcaccggtc tcgtcttcgc
     8941 accgggcacc gacggctcgc gtcggcggtt gtacctcacg gacaagggtg tcgacgggga
     9001 cttcaaccct gatccggtcc aggtcaacga cgggcagctg tatcagatgc ggctcgtcaa
     9061 cgtgtagcaa ggagtgttcc atcggttccg ggcgagatct ccaactcggc gccgagaccc
     9121 ttcggccaga gatcagcact gacctaagga acgccctcgg ccggggagtg gccattcgtc
     9181 ccggacgctg gcgggcttcc ggggtccctc cggccgaggg cgctcgaggc caggatagcc
     9241 cgaagcggga acactcgtcg gattcgtagt agacccggga cgaagacacg gtcgtccacc
     9301 cgagtggaca gcactcgagg agatgtccgc aggtagaggg gcatgtgctc cgtctgagtg
     9361 ccccctcacg ccgagcttcg acgcgacggc tatcgaatag gcgggagccg gtgaggcgcg
     9421 gcacggcctc gctccacccc cacgaccaga agaccggcac gaaccgagat ccgaacagat
     9481 ggtcgccttg agcggcactg tgacccccga cgtggtcgga gtccatgttc ggattgaatc
     9541 cacgcacgtg ggagagccga tctcatcgag gagcgcgatc ggcgtggaac cccggtcctc
     9601 aggttcccga accgtcggga cgtggtgatg attgcctccc cgcccttcac caccgtgccc
     9661 aaccggtcgg gcgctatcgc gcggatgctg cgtcagcctg ggatgttccg cgcggccaca
     9721 ccgtggcgca cgctcagcca cagccccgcg aggccccagg cgatcgcggc gacggccccg
     9781 accttcggga cagtcgaggc cgcagagccc gcgacaagtg aggcgacgcc gacaccgccg
     9841 gcagcggctg cggcgagggt tgccccgagc cgccagccac ggccgggtcg cgccgcggcg
     9901 gccatcacga ggatcaccat gaaggcgatg atcgccatgg cccaccagtg agcgtgtggt
     9961 gcggatcggc cgtcggcggg aacgtgttcc gctgaatgag ggcctggtcg attccgtacc
    10021 gcgccgcggg gatcgctccg accgcggcga gtgccagaag gtgtgtgcgc catcgatcgt
    10081 cggaacctcg gcgacgccag ggcgccgcga gcagcgccgc tacaacggcc ggcacggtga
    10141 ccagcagcag ggacgggtcg attgggctgg cctggccccc gacgttgtcc ggatcgccca
    10201 cgatggacag ggtgaccagc acgccggtca tcgccgcgag cacctggtac gcgtgcccgg
    10261 gccgctcggg ccggacgatc atcgctacga gacccgacgc tccgatcacg acaaccgcga
    10321 cggctccgac agcatggagc gcctcgccgg gatgagccgc cccgaaccgg gcctcggccg
    10381 acggaggcgg atcgtctggc gacccctcgg cacccgatga cacgacgccc agcgtgttca
    10441 gcgcgacgat cgagaagaag gacccgaaaa gcagcagacc gagcaccgcg acgatccgca
    10501 cggcccgtgt gcgaccgcgt gagagcggct gttccaatcg ctccatccct acatcatctc
    10561 cccgcgccgt ggagtgtctt acgttcggga tcgggttcga ctcctggtgt gacccggact
    10621 cccaagaatc gagcggccac tgcaggacca gcgatcggct agcccaggtg gaggaatcgg
    10681 agagactccg cgaattctct ccagactccc gtgcccccaa ggatgtgccc ccaggagtct
    10741 gagagttcac cggatagtgg acagatgtgt cggagcatcg tggacaccga tgacgggggt
    10801 ctgcgatgga tctggctggc tacgtggtgg atgcggtggt gctcgaggtc gcagctaccg
    10861 tgaggtggcg gcggcccacg gcgtgtccaa gagctacgtg ggcaagctgg tcgggcggtt
    10921 ccgagacggc ggctacggag cgatcgagcc gcggtctcga gcggcaaacg gatcccgttg
    10981 ttgatcgaag ggctcgacgt tcgcgtgatc tccgaggaag gggactgctt cgacacctca
    11041 cgctcgatcc gacccgggac taccaacccc aagggtcgtg acgctcgtct acgatgcgcc
    11101 gacacatccg gccacgatgg tccgacacat ctgtgaagtc tcaccacccg tgtcccggaa
    11161 tggctccggc acctatgcga catctcgggc caccttcctc ggcctgcctc caggattggc
    11221 gagcgccccg aactccttga gcttgtcatg gcgggctcgg tgagtgcgc
//
LOCUS       DASUYD010000168         3273 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_982603, whole genome shotgun sequence.
ACCESSION   DASUYD010000168 DASUYD010000000
VERSION     DASUYD010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_982603"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF195_09630"
     CDS             <1..646
                     /locus_tag="VF195_09630"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX6581117.1"
                     /translation="HVYLSDFGLTKRSASDSGITGTGQFVGTLDYAAPEQFKGGTPDA
                     RTDVYSLGCVLFECLTGSPPFTSENDAGLMYAHLQEPPPSLTARNAGLPREVDGVVAK
                     AMAKAPADRYPAAGALADEASRALGLSVGGVAAAYGRTRSRRFAPALAVAVALVVGVV
                     ASSILQGDATPADGGGAPASALAASRARSPLRSGPSSAPCPMPKSNCSPTFRKT"
     gene            643..2244
                     /locus_tag="VF195_09635"
     CDS             643..2244
                     /locus_tag="VF195_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581118.1"
                     /translation="MSSDCLPLDRDAPIHGERATIVCRTADVEVLYELFQTRDEMDDA
                     FQVNANNKAAPGGDCATDHLAVGTYTIGGEEAGRVLCYTVKRGSFTEELPPQSHIEWT
                     DENASIYAHAIRNDLGDLSLYEWWLTSAGPSSPEGSVPSKEAPRVTAGPRLKDGIFLA
                     APPGGCAGFDGETCALYIEEGSYKLMIKPLDTPANPAETGSLLLQKPDAVVFSPATGY
                     CFQGAQFGPPRSPRPAAYRWSGSSDAITFEKTEGGDCAGPQKMARAHVPWTRAPDGLV
                     AVEEGGEIELVDTGGSLERSTTQAETNPNSWPDWSPDASKIVYAGVAGADGFDLYVMN
                     LDGTATDQITDVLGDERTPAWAPEGDRIVFAFDDFGEPDYRSGLATVDPADSSWTEIF
                     VRENEDVDTPIWSPDGTRIAFTIFTNGVPAPYVIDADGGGLVKLRDDPGVVLAWTPDG
                     RRILVGGDGSFLTVRPDGSGERVFLEDPPEDGVLAVDWSPDGRWIVMSPPSGLGSTLY
                     LMRADGSQLFRIGFGTEPSWRPETP"
     gene            2250..>3273
                     /locus_tag="VF195_09640"
     CDS             2250..>3273
                     /locus_tag="VF195_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018562863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX6581119.1"
                     /translation="MRATDPLQEGVSASPGITLEELQLAQRNHGLHLEGLRYDVTPVG
                     MHYLLIHFDVPETDDRTWSLSIDGLVREPLELSMTDLRARPSATFPVTMECAGNGRAR
                     LSPRPISQPWLTEAIGTAEWTGTPLRPLLEEAGIRDEAVELVFAGADRGIQGEVEQNY
                     ERSLTLDDAMRDDVILAYAMNRGPIPPQHGFPLRLLVPGWYGMTSVKWLTRITAVAEP
                     FEGYQQLAYRFRQRPEDVGTPVTRMSPRSLMIPPGIPEFMTRRRLVAVGKQVLRGRAW
                     SGWGPIERVEVSADGGATWVDARLGESESPYAWFPWTFAWWASIGEFELCTRATDDAG
                     NTQPLEQ"
BASE COUNT          527 a         1098 c         1146 g          502 t
ORIGIN      
        1 gcacgtctac ctctccgact tcggcctcac gaaacgctcg gcgagcgatt cggggatcac
       61 cgggaccggg cagttcgtcg ggacgctcga ctacgcggcg cccgagcagt tcaagggggg
      121 cacgcccgac gcgcgcaccg acgtgtactc gctcgggtgc gtgctgttcg aatgtctcac
      181 aggctcgccg ccgttcacat ccgagaacga cgcgggcctg atgtatgcgc acctgcagga
      241 gccgcctccg tcgctcaccg cacggaacgc gggcctgccg cgcgaggtcg acggggtcgt
      301 ggcgaaggcg atggccaaag cgccagcaga ccggtatccg gccgcgggag ccctcgcgga
      361 tgaggcttca cgcgcactgg ggttgagcgt cgggggcgta gcagccgcat atggtcgaac
      421 gcgatcccgc aggttcgccc ccgcgctggc ggtggcggtc gcgctggtag tcggggtcgt
      481 cgcgtcgtcg atcctccaag gcgacgccac gccggcggac ggtggggggg cgcccgcttc
      541 ggcgctggcg gcatcgcgag cccggtcccc cctacgttcc ggaccgtcga gcgcgccctg
      601 tccgatgccg aagagcaact gctcacctac gttccggaag acgtgagctc cgactgcctg
      661 ccgctcgatc gtgacgcacc gatccacggc gagcgagcga ccatcgtctg ccgaaccgcc
      721 gacgtcgagg tgctgtacga gctgttccag accagggacg agatggacga cgcgttccag
      781 gtcaacgcga acaacaaagc agcccccggc ggggactgcg ccaccgacca tctcgccgtc
      841 ggcacgtaca ccatcggcgg cgaggaagcc ggccgggtcc tgtgttacac ggtgaagcgt
      901 ggctcgttca ccgaggagtt accgccccaa tcccacatcg agtggacgga cgagaacgcg
      961 tcgatctacg cgcacgcgat ccggaacgac ctcggggacc tcagcctcta cgagtggtgg
     1021 ttgacgtcgg cgggcccctc ctcgcccgag ggctcggtcc catcgaagga ggcgccgcga
     1081 gtcacggccg gtccccggct caaggacggc atcttcctcg cggccccgcc ggggggatgt
     1141 gcgggtttcg acggcgagac gtgtgccctg tatatcgagg agggctccta caagctcatg
     1201 atcaagcccc tcgacacgcc ggcgaatccc gcggagaccg gcagccttct cctgcagaag
     1261 cccgacgccg tcgtgttctc gcccgccacc ggttactgct tccagggtgc gcagttcggc
     1321 ccaccccggt ctccgcggcc cgcggcctat cggtggagcg ggagcagcga cgcgatcacg
     1381 ttcgagaaga cggagggagg agattgtgcc ggccctcaga agatggccag ggctcacgtg
     1441 ccgtggacgc gcgcgcccga cgggctggtg gccgttgagg agggcggcga gatcgagctc
     1501 gtggatacgg gaggctctct cgagcggtcg accacgcagg ctgagaccaa cccgaacagt
     1561 tggccggact ggtctcccga cgcctcgaag atcgtctatg ccggcgtcgc cggcgcggat
     1621 ggattcgatc tctacgtgat gaacctggac ggcaccgcca ccgaccagat caccgacgtg
     1681 ctcggcgacg agcgcacgcc tgcatgggcc ccggaagggg accggatcgt cttcgcgttc
     1741 gacgacttcg gcgaacccga ctatcgctcg gggctggcga ccgtcgaccc ggccgattcg
     1801 tcgtggacgg agatcttcgt aagggagaat gaggatgtcg atacccccat ctggtccccg
     1861 gacgggacgc ggatcgcatt cacgatcttc acgaacgggg tgcccgcgcc gtacgtcatc
     1921 gacgcggacg gcggcggcct cgtgaagctg cgcgatgacc ccggcgtcgt cctggcctgg
     1981 acgccggacg ggcggcggat cctggtcgga ggcgacgggt cgttcctgac cgtgcggccg
     2041 gacggatccg gcgagcgcgt gttcctggaa gacccccccg aggatggtgt gctcgctgtc
     2101 gattggtcgc cggacggacg ttggatcgtc atgtcgccac cctccggcct cgggtctacc
     2161 ttgtacctca tgagggcgga cgggagccaa ctgttccgga tcggtttcgg aacggaacca
     2221 tcctggagac ccgagacgcc gtgaccgaca tgcgggcgac cgatccgctg caggagggcg
     2281 tctccgcctc gccgggcatc accctcgagg aactgcagct cgcgcagcgg aaccacgggc
     2341 tgcacctgga aggcctccgc tacgacgtga cgcccgtagg gatgcactac ctcctgatcc
     2401 atttcgacgt gcccgagacg gacgaccgta cgtggtcgct ctcgatcgac ggactcgttc
     2461 gggagcctct cgagctctcg atgaccgacc tccgcgcccg tccgagcgcg acgttcccgg
     2521 tgacgatgga gtgcgccggc aacgggcgcg cgcgactatc gccacggccg atctcgcagc
     2581 cgtggctgac ggaggccatc ggcacggccg agtggaccgg gacgccgcta cgaccgctgc
     2641 tcgaggaagc cggtatccgg gacgaggcgg tcgagctcgt gttcgccgga gccgatcggg
     2701 gcatccaggg cgaggtcgaa cagaactacg agcgcagcct gaccctcgac gacgccatgc
     2761 gcgatgacgt gatcctcgcc tacgcgatga accgtggacc gatccccccg cagcacggtt
     2821 tcccgttgag gctcctggtc cccggttggt acggcatgac gagcgtgaag tggctcaccc
     2881 ggatcacggc ggtcgcggaa ccgttcgagg ggtatcagca gcttgcctat cgcttccgtc
     2941 agcggccgga agacgtcggg accccggtga cgcggatgag tccccgctcc ctgatgatcc
     3001 cgccagggat cccggagttc atgacgcggc gccggcttgt cgccgtcggt aagcaggttc
     3061 ttcgagggag agcatggtcg gggtgggggc ccatcgagcg cgtcgaggtc agtgccgacg
     3121 gcggcgcgac ctgggtggac gcgaggctag gtgaatccga gtcgccgtac gcgtggttcc
     3181 cgtggacgtt cgcgtggtgg gcgtcgatcg gagagttcga gctctgcacc cgagccacgg
     3241 acgatgcggg gaacacccag cccctcgagc agc
//
LOCUS       DASUYD010000169        15317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_541138, whole genome shotgun sequence.
ACCESSION   DASUYD010000169 DASUYD010000000
VERSION     DASUYD010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15317
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541138"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..966)
                     /gene="pth"
                     /locus_tag="VF195_09645"
     CDS             complement(391..966)
                     /gene="pth"
                     /locus_tag="VF195_09645"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004006136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX6581120.1"
                     /translation="MAWLVAGLGNPDDRYARTRHNLGRMVVDELAREEGERFRKVRFL
                     PVESAEIRVADGRVILARSTRYMNESGPSYASLAKKQGIEPADVIAVHDELDILAGTI
                     RIKRGGGNSGHNGLRSLDQAFRTPDYLRVRVGIGRPPGRQDPSDFVLQPVGAREETDL
                     ALLVSHSADAVRALITDGLERTQDRFNRSTP"
     gene            complement(970..1716)
                     /locus_tag="VF195_09650"
     CDS             complement(970..1716)
                     /locus_tag="VF195_09650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX6581121.1"
                     /translation="MEAILQAEPRTETGKGPAHRLRASGRVPAVLYGAGVDATPIHVS
                     ALDLLHVFHAMGQASVLVDLKLNGNEYLTIAREIQRDLIHNRFIHIDFMAVNRDEKIT
                     VSVELHEVGDSEGVHAGGVIEHHLREVEIECLPANVPPRVVFDISKLKIGDSVRIGDL
                     VPPEGVEFLTDPDTMILAVVEPAVMDTELDLAVAGEEAPEEAAEAAEEAEGEEAAEGE
                     EAPEGEGAEGGEGTGADGGGQAPTEGDGES"
     gene            1789..3147
                     /locus_tag="VF195_09655"
     CDS             1789..3147
                     /locus_tag="VF195_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6581122.1"
                     /translation="MRPARAVGALLVSAVLAATCQDPPRARDTPDLLPPGRPERTDAS
                     AFETRWPIKHVVFVIKENRTTDHLFGRFPGVNGVTEGYDGDDLRPLTPPPDRLPDDIP
                     HCYECGLESWNEGRMNRFARNDISDRFAYTQMQGPENLPNYWRWAEEFALSDNFFSSA
                     QGPSFPNHLYTIAAQSGGSHHNPFQDQARATLFTQETGLAKSWGCDTLEEVYVEVVDS
                     EGEVERVPPCFDFQTEADLLDRADIPWGYYAAWNDQRGYVWSAFSAIERVRMNPEIWQ
                     RHIFPVDNFVADVDEGRLAPVTWVTPRYELSEHPEWSFCHGENWTTEIVNAVMRSRFW
                     KDTAIFITWDDYGGFYDHVPPPQVDDFGFGFRVPLLTISPFAKPGYIDSQLGEFSSVL
                     RFIEDNWGLSQLTHRDRDAFNLSYLFDFDQRPREPFELPLRDDCVGPRLHKLDSVDPD
                     GE"
     gene            3227..3727
                     /locus_tag="VF195_09660"
     CDS             3227..3727
                     /locus_tag="VF195_09660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6581123.1"
                     /translation="MAELKGELADLYVRHVPAANRLAFLLTGDGSHAEDLVHDAFVRC
                     VGRFGHLRAHEAFDAYLRRTIVNLHTSRLRRVRVEREFLSKEAGRRARGETATMADVD
                     AREDMWRRLGTLPPRQRAVLVLRYYEDLSERETADALRCSVAAVKSLTARATSALREG
                     IRGEDG"
     gene            3724..5697
                     /locus_tag="VF195_09665"
     CDS             3724..5697
                     /locus_tag="VF195_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581124.1"
                     /translation="MTPRIDHGDFENELRDLFREKAGEAPLATPSLPASAPRQVLRRG
                     RIHQLGTVLGSAIVVVALIVGSVAGLTRILGEGEKDRVGSDDYEVFPRTATIEAFIVG
                     SPSDWYLVNEWPLSMLIAVEGSGGSSSACGAVPGDGVEECIDTVEETSSPIPVPSGLP
                     MLQLSNVDLGLGTNACEGELPADAAVLYVAYDQDVAANPRAAPSPDPFPPGERGLPPV
                     GSGPCGPGRYAHFTVNGEPFFTWIGVGARVTADDREIVETSYEMMSAIPDWELSPPAE
                     TTPAYVIDGGLTQAGTDWRLELRPSQQNVALSLVGVARSSDFVIPPSVIEWAATDPIF
                     GAISKQAAAVEFRGKDDPSIAIPGTIVPLPPSMPFDFDLFFIEGTAGIEGEPVALGPD
                     GEPIEGPSSVGTVRDDVVRLEGSTLGHDWMARFLGAFEDDTACIDVTIDDEGSEPLCP
                     SPIATSLAGAQPSLHVVNTADLAVVVGSVPPEVVEIRFTSDSGSTTPSRFPCQMGPVG
                     WTDPDRSVCALALPPQDGGTFEYLGSDGDVLFEEGMAWFSAQGEPVAPTPVDPVHGGT
                     YWAVYPWLGAAGSPDAENVSALLLEDFGIEAFPGDLSCDDGAAEALGTDAPQGIGVYF
                     ETEDEANAFADQAGLLGHEADPVIARVTTYCLD"
     gene            5731..6657
                     /locus_tag="VF195_09670"
     CDS             5731..6657
                     /locus_tag="VF195_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016188331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX6581125.1"
                     /translation="MDDHARRQSWFGLKRFVNRFLTLRVPNAFLRLLSKGAPSLRSGR
                     LPAPSRLTEVAGRVGGVDFVMLDPARCENAKELFWGRGRRPNAHDRLALDTVAALARG
                     SDTLLDVGAYTGLFTMAVTAYAPHVDVHAFEIVPAVADALEANAHRNGVEDRVVVHRE
                     GIGDPDKTMRVPSGEGGSALPSFYSSRMRFEGGKEVDFRSLDSVAALIPEASRVVMKV
                     DVEGTEDAVFRFGQAFLRTCRPHILCEVLHDVADGAALDGLLAGARLHKYLVTDTRLA
                     RIDRIVPHPAFRDWLFTSLTEDDLRGLDLPVG"
     gene            complement(6654..7634)
                     /locus_tag="VF195_09675"
     CDS             complement(6654..7634)
                     /locus_tag="VF195_09675"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0044249 - cellular biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX6581126.1"
                     /translation="MEIITKKRMMLFSGTIHPALGSEIAECLGIPVSRSELRRFASGE
                     IYFRAEESVRGADVFVVQSHYEPVNEALMEQLIMIDAMKRASAKRINAVIPFYGYSRQ
                     DHKALSREPISAKLVADLLSTAGADRVISVDLHSGQIQGYFDFPFDHLTALPILSDHF
                     KDELGLHGDDLVVVAPDAGRIKTSEKLREYLHADLAFLYKRRSRTEAHKIEEMAVVGE
                     VEGRPCVIVDDMIDTAATISKGVQVLAEIGAGPIFVGATHGLFSGKARQLLEEAPIEQ
                     VVVTNTVPIPEERTFAKLKVLSIAPLIANGLRAVFEDASVSAIFGGDNQV"
     gene            complement(7644..9068)
                     /gene="glmU"
                     /locus_tag="VF195_09680"
     CDS             complement(7644..9068)
                     /gene="glmU"
                     /locus_tag="VF195_09680"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX6581127.1"
                     /translation="MAASTEKDARRSLAAVILAAGQGKRLRSGIPKVLHPACGRPILW
                     HTLQVVRAAKPSRIVVVVGYGADDVREAVESWGIEPAPVFVEQAERLGTGHAVQLAED
                     AIGEAADVLVANGDFDPVTVADVRKLLSSHRRSGAAVTLGSTVLDAPGGYGRVVRDGR
                     RVVDVVEGRDAPPAVRKIHEVATNWIAFRRDLLYATLPLLDRENRQREYYLNRAISIL
                     LDKGERVDAVVCDTGGVLGANTRAGLAGLETLIRRRTNDFHMAAGVRLIDPSATYIDV
                     EVRLGKDTVVYPNTFLERGVRIGAGCRIGPSTAITRSRVGDRSIVWFSVLEDARVGKD
                     CEVGPFARLRPGAILQDGALVGNFVEVKGSRIGRGSKAKHLTYIGNAELGVGVNVGAG
                     TVVVNYDGYDKHPTVIGDGARIGSDTMLVAPLKVGKGAVTGAGSVITKDVPAGALAVE
                     RSDQKNVKGYRKRADAKHRSKGEH"
     gene            complement(9279..12413)
                     /locus_tag="VF195_09685"
     CDS             complement(9279..12413)
                     /locus_tag="VF195_09685"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6581128.1"
                     /translation="MRTCQTCGTSNPDTARFCNACGERLAAQEATPRRRIVAALFCDL
                     VGSTELAERTDPEVLKRIMDRYFGTMRASIERHGGTVEKFIGDAVVGAFGIPTSHEDD
                     ALRAVRAALEMRAAADELDRDVGIDARIRVRIAIDAGETFAEDGAAIEGRVAGDTFNT
                     AARLQSNANVGDVLVSGPAERLARRSLRTERLPPLTLKGKTEPVDVFRVLSIYEAPSL
                     TQTTFLGRSRAITSLEHALEDAIEAESCVLVTVLAPPGVGKSRLAETFADTVDGRARV
                     LTAQTPSYGEGVTFSPLIELLADAAGIQAADAEAVASSLRRRLAGEPDGAAVADRLAQ
                     ILGVHEALAADASWAVRRLLEALSRDRPLVVILDDTHWAEEPMLDLVDTVVDRFHGPL
                     LVVCLARPELLDRRPTWAAGKSRAVTTTLPPLEPPDARRLAETLLPDAPGSVIDRVCE
                     AAEGNPLFLEQLVAMLIDRGSLVEGRWLGAEDDEIDIPSTVQALLAARIGDLEPAARG
                     ILEHASVEGRRFRMAAVRALAEGVDEESFDHGLVLLERRGFVDLDDEAAGRWRFAHAL
                     IAEAAYRGISKELRATLHERLAAWLLSEEAEHPDVDESAARHLERAFHLYEELGVRNE
                     AADALAERAGVLFAEAGGRAFAGVDLIAARDLLGRAARLLPQDSSRRLDLLPNLGVAL
                     TETGRPEETEALLVGAVEQARSAGADAAALRASIQLLANRVYRSPTQPEIVAAAADAH
                     AAAEALEALGDRVGLAEAAIAIEYLGWMRGDLEEHRVWGVLALRHGLAAGRPREAAQG
                     AADAVLATAFGRTPFDGFPSVADEIDSIAKHPLTMSASAALRAMAALGAGDDDAFDRH
                     ERDWRDVLDHHGLSWVSAAHGLVMAALEMWVGRAAIAEQRLREARDVLAAAGDVWWID
                     SIDALLGSALATQGRRREFLTHVDAFENSNPVPDRDSLVRRPLLRSRAFLMRGSTADA
                     EDAARSAVSAAAGSDLVLSLAEANLALADVLEARGRPDDAEAARGHAAGILEAKGFRA
                     ALNHVAGADQGTRT"
     gene            complement(12563..12633)
                     /locus_tag="VF195_09690"
     tRNA            complement(12563..12633)
                     /locus_tag="VF195_09690"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12599..12601),aa:Gln,seq:ttg)
     gene            complement(12619..13509)
                     /gene="ispE"
                     /locus_tag="VF195_09695"
     CDS             complement(12619..13509)
                     /gene="ispE"
                     /locus_tag="VF195_09695"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEX6581129.1"
                     /translation="MPAHAKVNVFLRVIGRRDDGYHDIETLLLPISLADHVRVEPADE
                     LSVSVEGSAAAGLPTDDANLALRAARALAAEVGGASPVGAHITIDKRIPVAAGLGGGS
                     ADAAATLLLLDELWSTGLGRDGLSRLAAEIGSDVPALLLGEPAYAHGRGERVAAVLVQ
                     STTWVVKPFAFGVSSADAYAWWDEEPATGPDAGALIAAAEAGNDALVGSALTNDLQGP
                     VAARHPEIVATVETFLDTGAHGAVMTGSGPTVVALCSFAVAEDVAGSVPGAYVVEAPP
                     RAPTPARIDHGSEAGPSGVV"
     gene            complement(13536..14402)
                     /gene="rsmA"
                     /locus_tag="VF195_09700"
     CDS             complement(13536..14402)
                     /gene="rsmA"
                     /locus_tag="VF195_09700"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013839615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEX6581130.1"
                     /translation="MSTAEHASFGAGALRALAERHGIRPKRSLGQHFLIDPNLARAIA
                     ADAGVGPGDRCVEIGAGLGSLTRALAEAGAEVLAVEVDPSLISALEESVAGLDRVRVL
                     HTDATDPEWQDALDGDGWVLAANLPYNIATHIVLDTLRDVPRVRRLVVMVQREVGERL
                     VAAPGEPAYGIPSVRVAYRAEGTLERRVPPSVFWPRPKVESVIVRLVRRDEPAVAVDE
                     HRLWAVVDAAFGERRKTMRNALRRLGLEGEVADELLEAAGVDPSARGETLSLEEFARI
                     AESFPEDTAPPR"
     gene            complement(14918..>15317)
                     /locus_tag="VF195_09705"
     CDS             complement(14918..>15317)
                     /locus_tag="VF195_09705"
                     /inference="COORDINATES: protein motif:HMM:NF013213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX6581131.1"
                     /translation="KPMVVHVRDAWPDVLRVLDEGSAERVVIHCFSGDAAIAKECAER
                     GYWISFAGNITYPKNEHLRQAARAVDIDRILVETDAPFLAPQTLRGTDNEPANVIHTI
                     AEVARIRDEDVRSVSEATSRNADAVFFGLS"
BASE COUNT         2328 a         5403 c         5226 g         2360 t
ORIGIN      
        1 cagccggcac ccaccgccat cgcgagggcg gccagccaag cgagcgcgta cgcgatcttg
       61 cggagtcgca tgcgtctctc cttcgtggtc ggacctgcgt cagcagggct tccagcgagc
      121 gtcgacggag acctggggaa ccgtaacggt gcgcatctcg tcgacctcct tgtccatcga
      181 ggtcgaggat gaccgtcgca cgggggtcgg gtcatcgggc gacggtcccg agtttccatc
      241 ggccgggcac ccccgacctt cgtcgggggt ctgctcccat cgcgggtgat gcggataggc
      301 cgatggccca acccccatcg caccgaaggg gagggtgggt agtcctcccg aaccggtgtc
      361 agccgtcttg ggggtgaggc tcccgtcggc tcaaggggtc gagcggttga accgatcctg
      421 ggtgcgctcg agcccgtccg tgataagcgc gcggacggca tcggcggagt ggctcacgag
      481 cagcgcgaga tccgtctcct cccgcgcccc caccggctgc agcacgaaat ccgatgggtc
      541 ctgccgtccc ggcgggcggc cgatccccac gcgcacacgc aggtagtcgg gggtgcggaa
      601 ggcctggtcg agcgaacgga ggccgttgtg gccgctgttg ccgccccctc gcttgatccg
      661 gatcgtgccc gcgaggatat ccagctcgtc atggacggcg atcacatcgg cgggctcgat
      721 cccctgcttc ttggccagcg aggcgtagga cggccccgac tcgttcatgt accgcgtgga
      781 gcgcgcgagg atgaccctcc cgtccgccac acggatctcc gcggactcga cggggaggaa
      841 acgcaccttc cggaaccgct cgccttcctc gcgagccagc tcgtcgacga ccatccggcc
      901 gaggttgtga cgcgtcctcg cgtagcgatc gtccgggttt ccgaggccgg cgacgagcca
      961 ggccatcggc tagctctcgc cgtccccctc ggtgggggct tggccgccgc cgtcggcccc
     1021 ggtcccttcg ccaccctcgg cgccctcgcc ctcgggagcc tcttcgccct cggcggcctc
     1081 ttcgccctcc gcttcctcgg ccgcctcggc cgcctcttcc ggggcctctt cgccggcgac
     1141 cgcgaggtcg agctccgtgt ccatgaccgc gggttccacg accgcgagga tcatcgtgtc
     1201 ggggtccgtg aggaactcga caccttcggg cgggacgagg tcaccgatgc gcaccgaatc
     1261 gccgatcttc agcttggaga tgtcgaacac gacccgcgga ggcacgttcg ccggaaggca
     1321 ctcgatctcc acctcgcgga ggtgatgctc gatcacaccg ccggcgtgca cgccctcgga
     1381 atccccgacc tcgtgcagct cgacgctcac ggtgatcttc tcgtcacggt tgaccgccat
     1441 gaaatcgatg tggatgaaac ggttgtggat caggtcgcgc tggatctcgc gggcgatcgt
     1501 caggtactcg ttcccgttga gcttcaggtc gacgaggaca ctcgcctggc ccatcgcgtg
     1561 gaagacgtgc aggaggtcga gggcgctcac gtggatcggc gtggcatcga cgccggcgcc
     1621 gtacaggacc gcggggacgc gaccgctggc gcggagacga tgcgcgggcc ccttcccggt
     1681 ctcggtgcgg ggctcggcct gaagtatggc ttccatggca ggtcagaggt ccttctcgaa
     1741 ggggtcgatc cggctggtca tcgtaccagc ggctagcatc ccgcttcgat gcgacctgcc
     1801 cgcgccgtgg gcgcgctgct cgtctcggcc gtgctggcgg cgacctgtca ggacccgccc
     1861 cgcgcgaggg acacgccgga cctcctccct cccgggagac cggagcgcac cgacgcctcc
     1921 gccttcgaga cgcgttggcc catcaagcac gtggtgttcg tgatcaaaga gaaccggacg
     1981 acggatcacc tgttcggtcg gttcccagga gtgaacggtg tcaccgaggg gtacgacggc
     2041 gacgacctgc gaccgctcac gcctccgccg gatcgtctgc ccgacgacat ccctcactgc
     2101 tacgagtgcg gactcgagtc ctggaacgag gggcggatga acaggttcgc gaggaatgac
     2161 atcagcgacc gcttcgcgta cacgcagatg caggggcccg agaacctccc caactactgg
     2221 cgatgggccg aggagttcgc gttatcggac aacttcttct catccgccca gggcccgtcg
     2281 ttcccgaatc acctgtacac gatcgccgcg cagtcgggcg gctcgcacca caaccccttc
     2341 caggaccagg cccgagcgac gctcttcacg caggagaccg gcctcgcgaa gtcgtgggga
     2401 tgcgacacgc tcgaggaggt ctacgtcgag gtcgtcgaca gcgaggggga ggtggaacgg
     2461 gtgccgccgt gcttcgactt ccagaccgaa gccgacctcc tggatagggc ggacatcccg
     2521 tggggctact acgccgcgtg gaacgatcag cgaggctacg tctggtcggc gttctcggcg
     2581 atcgagcgcg tgcggatgaa cccggagatc tggcagcgcc acatcttccc ggtggacaac
     2641 ttcgtcgccg acgtcgacga ggggcgtctg gccccggtca cgtgggtcac accgcgctac
     2701 gagctgagcg agcatcccga gtggagcttc tgccacgggg agaactggac cacggagatc
     2761 gtcaacgccg tgatgcggag ccggttctgg aaggacaccg cgatcttcat cacgtgggac
     2821 gactacggcg gcttctacga ccacgtgccc ccgccacagg tcgacgactt cgggttcggc
     2881 ttccgcgtcc cgctgctcac gatcagcccc ttcgcgaagc cgggctacat cgactcgcag
     2941 ctcggtgagt tctcgagcgt ccttcggttc atcgaggaca actgggggct ctcccagctc
     3001 acccaccggg accgggatgc gttcaacctg agctacctct tcgacttcga ccaacggcct
     3061 cgggagccgt tcgagcttcc tcttcgcgac gactgcgtgg gcccgaggct ccacaagctg
     3121 gacagcgtgg atccggacgg agagtgatcc ggcgatacct tccgggcgcc tcgtgcgtct
     3181 aggaggtatg acaaggatcc tcctcggggc gcaggaagga acggcggtgg ctgagctgaa
     3241 gggcgagctg gcggacctgt acgtccgtca cgtgcccgcg gcgaaccggc tcgcgttcct
     3301 cctgaccgga gacggctcgc acgctgagga cctcgtccac gacgcgttcg tgcggtgcgt
     3361 cggtcggttc ggccatctcc gtgcgcacga agcgttcgac gcgtacctgc ggcgcacgat
     3421 cgtgaatctc cacacgtcga ggctgcgccg cgtccgcgtc gagcgcgagt tcctctcgaa
     3481 ggaggcgggg cggcgtgccc gcggcgagac ggcaacgatg gcggacgtcg acgctcgaga
     3541 ggacatgtgg cgacgactcg gaacgctgcc accgcgccag cgagcggtgc tggtgctccg
     3601 ctactacgaa gacctgtcgg agcgggagac ggccgatgcg cttcggtgtt cggtcgccgc
     3661 cgtgaagtcg ttgaccgcgc gcgccacgtc ggcgctccgc gaagggatca gaggtgagga
     3721 cggatgacgc cacggatcga tcacggggac ttcgagaacg agctcaggga cctgttccgc
     3781 gagaaggccg gcgaggcgcc gctcgcgacg ccgagcctcc cggcgtccgc gccgcggcag
     3841 gtgctgcgcc gcggccggat ccatcagttg gggacggtgc tcggctccgc gatcgtcgtc
     3901 gtcgcgctga tcgtcggctc ggtggccggg ctcacccgca tcctgggtga aggcgagaag
     3961 gaccgcgtcg ggagtgacga ctacgaggtc ttcccacgca ctgcgacgat cgaggcattc
     4021 atcgtgggaa gcccatccga ctggtacctg gtgaacgagt ggcccctctc gatgctgatc
     4081 gccgtcgagg ggagcggcgg ttcctcctcg gcctgcggcg cggtgccggg tgacggggtc
     4141 gaggagtgca tcgacacggt cgaggagacg tcgtcaccga tccccgtgcc ttccggcctg
     4201 ccgatgctcc agctctcgaa cgtcgacctg ggcctgggga cgaacgcatg cgagggtgaa
     4261 ctcccagcgg acgccgccgt gctctacgtc gcctacgatc aagacgtcgc cgcgaacccc
     4321 cgggctgctc cctccccgga cccgttcccc ccaggcgagc gtgggcttcc ccccgtcgga
     4381 agcggcccgt gcgggcccgg gcgatacgcc cacttcaccg tgaacggtga gcccttcttc
     4441 acctggatcg gagtcggagc gagagtcacc gccgacgacc gagagatcgt cgagacctcg
     4501 tacgagatga tgtcggcgat ccccgactgg gagttgtcgc ctccggccga gacgacccct
     4561 gcctacgtga tcgacggggg tctcacgcag gccgggaccg actggcggct cgaattgcgc
     4621 ccctcgcagc agaacgtggc gttgtccctg gtcggcgttg ctcgatccag cgacttcgtg
     4681 atcccgccgt ccgtgatcga gtgggcggcc accgacccga tcttcggcgc gatctccaag
     4741 caggccgccg ccgtggagtt ccgcggcaag gacgatccat ccatcgcgat cccgggaacg
     4801 atcgtgccct tgccgccaag catgcccttc gacttcgatc tgttcttcat cgaaggcacc
     4861 gcgggcatcg agggcgagcc cgtcgcgctg gggccggacg gcgaaccgat cgagggcccc
     4921 tcgagcgtcg ggaccgtgcg ggacgacgtc gtccgcctcg aaggatcgac gctcggacac
     4981 gactggatgg cgcgattcct cggcgccttc gaggacgaca ccgcgtgcat cgacgtcacg
     5041 atcgacgacg agggaagcga gccgctctgt ccgagcccca tcgcgacgtc gctcgcaggt
     5101 gctcagccgt ccctgcacgt cgtgaacacc gccgacctcg cggtggtcgt cggctcggtt
     5161 ccgccagagg tggtggagat ccgattcacg agcgacagcg ggagcaccac gccttcccgg
     5221 ttcccgtgcc agatgggacc ggtcggctgg accgatcccg atcgcagcgt gtgcgctctc
     5281 gccttgcccc cgcaggacgg agggaccttc gagtacctcg gctcggacgg cgatgttctg
     5341 ttcgaggaag ggatggcgtg gttctccgct cagggtgagc cagtggctcc gacgccggtc
     5401 gatcccgtcc acggcggcac ctactgggcc gtgtatccat ggctcggggc tgcgggatct
     5461 cccgacgcgg agaacgtgag cgccctgctc ctcgaggatt tcgggatcga ggccttcccc
     5521 ggcgacctct cgtgcgatga cggggcggcg gaagcgctcg ggacggacgc tccgcagggc
     5581 atcggcgtct acttcgaaac ggaggacgag gcgaacgcgt tcgcggacca ggctggactg
     5641 ctcggacacg aggcagatcc cgtgatcgcg cgcgttacca cgtactgcct ggactgaccg
     5701 accgctcacg cggtttggca ggatggctgt gtggacgatc acgcccgccg gcagagctgg
     5761 ttcggtctga agcgcttcgt caatcgcttc ctcacgcttc gggtgcccaa cgcgttcctt
     5821 cggctcctct cgaaaggagc gccgtccctg cgctcgggtc gcctccccgc gccgtctcgc
     5881 ctcacggaag tagccggtcg cgtgggcgga gtcgatttcg tcatgctcga cccagcgcgc
     5941 tgcgagaacg cgaaggagct cttctggggt cgcggccgtc ggccgaacgc ccacgatcga
     6001 ctggcgctgg acacggtggc cgcgctcgct cgaggatccg acacgttgct cgacgtgggc
     6061 gcgtacacgg ggctgttcac gatggcggtg acggcctacg cgccgcacgt cgacgtccac
     6121 gcgttcgaga tcgtcccggc cgtggccgat gcattggagg cgaacgcaca tcggaacggg
     6181 gtcgaggatc gcgtcgtggt ccatcgggaa ggcatcgggg atcccgacaa gacgatgcgc
     6241 gtcccgtcgg gcgaaggggg ttcggcactc ccctccttct actcgtcgag gatgcgattc
     6301 gagggaggca aggaggtcga cttccgatcg ttggactccg tggccgccct gatcccggag
     6361 gcttcgcggg tcgtgatgaa ggtggacgtg gagggaacgg aggacgcggt cttccgcttc
     6421 ggtcaggcgt tcctgaggac ctgccgtccg cacatcctgt gcgaggtgct ccacgatgtc
     6481 gccgacgggg cggccctcga tgggctgctc gctggtgcga ggctccacaa gtacctcgtc
     6541 accgacaccc ggctcgcgcg gatcgatcgc atcgtgccgc acccggcgtt ccgcgactgg
     6601 ctgttcacct ctctcaccga ggatgatcta cgcggtctcg atctcccggt cggctagacc
     6661 tggttgtcgc cgccgaagat cgccgagacc gaggcatcct cgaagaccgc tcggaggccg
     6721 ttcgcgatca acggcgcgat cgagaggacc ttcagcttgg cgaacgtgcg ctcctcgggg
     6781 atgggcaccg tgttcgtcac gacgacctgc tcgatcggcg cctcctccag cagctgacgc
     6841 gccttgccgc tgaacaggcc gtgggtcgcc cccacgaaga tcggcccggc gccgatctcg
     6901 gcgaggacct ggacgccctt cgagatcgtg gccgcggtgt cgatcatgtc gtcgacgatc
     6961 acgcacggcc tgccctcgac ctcaccgacg accgccatct cctcgatctt gtgggcctcc
     7021 gtgcgggagc ggcgcttgta gaggaacgcg aggtccgcgt ggaggtactc gcggagcttc
     7081 tccgaggtct tgatccgccc ggcgtcgggc gccaccacga ccaggtcgtc gccgtgcaac
     7141 cccagctcgt ccttgaagtg gtccgacagg atcgggagcg ccgtgaggtg gtcgaagggg
     7201 aagtcgaagt agccctggat ctgccccgag tgcaggtcga ccgagatcac ccggtcggcg
     7261 cccgcggtgg acaggagatc ggcgacgagc ttcgcactga tcggctcgcg ggacagggcc
     7321 ttgtggtcct ggcgggagta gccgtagaac gggatcacgg cattgatccg cttggccgac
     7381 gctcgtttca tggcgtcgat catgatcagc tgctccatca gtgcttcgtt caccggctcg
     7441 tagtgggact gcaccacgaa cacgtcggcc ccccgcacgc tctcctcggc acggaagtag
     7501 atctcccccg acgcgaagcg ccggagctcg ctgcgactca ccgggatgcc gaggcactcg
     7561 gcgatctcgc tccccagcgc cggatggatc gttccggaga acagcatcat ccgcttcttg
     7621 gtgatgatct ccacggtcgc cccctagtgc tcgcccttcg accgatgctt ggcgtcggcg
     7681 cgcttccggt accccttcac gttcttctgg tcggaccgct caacggccag cgcgcccgcc
     7741 ggcacgtcct tcgtgatcac cgatccggcc cccgtgacgg cgcccttgcc gaccttcagc
     7801 ggcgccacca gcatcgtatc cgacccgatc ctcgccccgt cgccgatcac ggtggggtgc
     7861 ttgtcgtagc cgtcgtagtt cacgacgacg gtcccggccc cgacgttcac ccccaccccg
     7921 agctcggcgt tgccgatgta cgtgaggtgc ttggccttcg accccctgcc gatccgcgag
     7981 cccttcacct ccacgaagtt gccgacgagg gcaccgtcct gcaggatcgc cccgggccgt
     8041 agacgcgcga acggaccgac ctcgcagtcc ttcccgaccc gggcgtcctc gaggaccgag
     8101 aaccacacga tcgatcggtc gccgacccgg gagcgggtga tcgcggtgga cggaccgatc
     8161 ctgcagccgg cgccgatccg gacccccctc tccaggaacg tgttcgggta gacgaccgtg
     8221 tccttgccca gacggacctc gacgtcgatg taggtggcgg agggatcgat cagacggacc
     8281 ccggcggcca tatggaagtc attggtccgg cgacggatca gcgtttcgag accagcgagc
     8341 ccggcccggg tgttcgcgcc caggaccccg cccgtgtcgc agacgacggc gtcgacacgt
     8401 tcgcccttgt cgaggaggat cgagatcgcg cggttcaggt agtactcgcg ctggcggttc
     8461 tcacggtcca gcagcggcag cgtcgcgtac aggaggtccc ggcggaacgc gatccaattc
     8521 gtcgcgacct cgtggatctt ccggacggcg ggcggcgcat cgcggccctc gaccacgtcg
     8581 acgacgcgcc gaccgtcgcg tacgaccctc ccgtagccgc cgggcgcgtc gaggaccgtg
     8641 gagccgaggg tgacggcagc gccggagcgc cgatgcgagg acaggagctt ccggacgtcg
     8701 gcgacggtga ccggatcgaa gtccccgttc gccaccagga cgtccgcggc ctcgccgatg
     8761 gcgtcctcgg cgagctggac cgcatgaccg gtcccgagcc gctcggcctg ctcgacgaag
     8821 acgggcgctg gctcgatgcc ccacgactcg acggcctcgc ggacgtcgtc ggcgccgtag
     8881 ccgacgacga cgacgatcct cgagggcttc gcggctcgca cgacctggag ggtgtgccac
     8941 aggatcggac ggccacaggc cgggtggagc accttgggga tgccagaccg cagccgcttc
     9001 ccctggccgg cagcgaggat cacggcggcg agcgatcgtc gggcatcctt ctcggtggag
     9061 gcggccactg ctcatccttc ggcgtcgcgg ggggggaagg tcgcgacctc atcgtcccac
     9121 acgccgacga cgcccgcccg cccccggccc ttgctcggtc acacgaaggc gtggaggcgc
     9181 aaggcggcga ggtgatcgga ctgcgagggg acgaggcgct cgccgtgttc gagagcgcgc
     9241 cgcggcgctc cgggccgaga cggggtcccg accctggcct acgtccgcgt gccctggtcc
     9301 gctccagcca catggttcag cgccgctcgg aaccccttgg cttccaggat gccggccgcg
     9361 tgtccccgag cggcctccgc gtcgtcggga cgaccgcggg cctcgagaac gtcggcgagc
     9421 gccaggttcg cctccgccag tgaaaggacg aggtcggacc ccgcggccgc ggagacagcg
     9481 cttcgcgccg cgtcctcggc gtcggccgtc gagccgcgca tgaggaacgc gcgcgagcga
     9541 agcagcggac gccgcaccaa cgagtcgcga tcgggaaccg ggttggagtt ctcgaaggcg
     9601 tccacgtgcg tcaggaattc ccggcgacga ccctgtgtcg ccagtgcact tcccaacagc
     9661 gcgtcgatcg agtcgatcca ccagacgtcg ccggccgccg ccagcacgtc tcgcgcctcg
     9721 cgcaatcgct gttcggcgat cgcggcacgt ccgacccaca tctccagggc cgccatcacg
     9781 agcccgtgag ccgccgagac ccacgacagg ccgtgatgat cgagcacgtc gcgccagtcg
     9841 cgctcgtggc ggtcgaacgc gtcgtcgtcc ccggcaccca gcgccgccat cgcgcgcaag
     9901 gcggccgacg cgctcatggt gagtggatgc ttcgcgatcg agtcgatctc gtcggcgacc
     9961 gaggggaagc cgtcgaacgg cgtccggccg aacgccgtcg cgaggaccgc atccgcagcc
    10021 ccctgcgccg cctcgcgcgg tcgtccggcg gcgagaccgt gccggagcgc cagcaccccc
    10081 cacacccgat gctcctcgag gtcgccgcgc atccacccga gatattcgat ggcgatcgcc
    10141 gcctcggcga gcccgacccg gtccccgagc gcctcgagag cctccgccgc cgcatgcgcg
    10201 tcggcggcag ccgccacgat ctcgggctgg gtcggtgacc ggtacacgcg gttcgccagg
    10261 agctggatgc tcgcccgcag cgctgcggcg tcggcgccgg cggaacgcgc ttgctccacg
    10321 gcgccgacca gcaacgcctc cgtctcctcg ggccgacccg tttcggtgag agcgaccccg
    10381 aggttcggga ggaggtcgag tcggcgagac gagtcctgcg gcagcagccg tgccgcgcgt
    10441 ccgagcagat cgcgcgccgc gatcaggtcc acacccgcga acgcgcgccc ccctgcctcg
    10501 gcgaagagca ccccggcgcg ctccgcgagc gcatccgccg cctcgttccg gacgcccagc
    10561 tcctcgtaca ggtggaaggc gcgctcgaga tggcgggcgg ccgactcatc gacgtcgggg
    10621 tgctcggctt cctcggacag gagccatgcc gcgagccgct cgtgaagcgt tgcccggagc
    10681 tccttcgaga tgccccgata cgccgcctcg gcgatcaggg catgcgcgaa ccgccaccgt
    10741 ccggccgcct cgtcgtcgag gtcgacgaat ccgcggcgtt ccagcagcac gagtccgtgg
    10801 tcgaacgact cctcgtcgac accctcggcg agcgctcgca ccgcggccat gcggaaccgt
    10861 cggccttcga ccgacgcgtg ctccaatatc cctcgggccg caggctcgag gtcaccgatc
    10921 cgggccgcga ggagcgcctg cacggtcgaa gggatatcga tctcatcatc ctccgcgccg
    10981 agccatcgac cctccacgag ggatccacga tcgatcagca tcgcgaccag ttgctcgagg
    11041 aagagcggat tgccttcggc cgcctcgcag acgcggtcga tgacggaccc gggggcgtcg
    11101 ggaagcagcg tttccgccag ccgccgagcg tcggggggct cgagtggagg gagcgtcgtc
    11161 gttacggcgc gcgacttccc ggctgcccat gtcggtcgtc gatcgaggag ctcgggacgg
    11221 gcgagacaga ccacgaggag cgggccgtgg aacctgtcca ccaccgtgtc gacgaggtcg
    11281 agcatcggtt cctctgccca atgcgtgtca tcaaggatca cgacgagcgg ccgatcccgc
    11341 gacagcgctt cgagcaggcg gcgaacggcc cacgacgcat cggcggcaag cgcctcatga
    11401 acgccgagga tctgggcgag tcgatccgca acggcggcgc cgtcgggctc gcccgccaga
    11461 cgtcgtcgga ggctcgaggc gaccgcctcg gcgtcggccg cctgtatgcc ggcggcatcg
    11521 gcgaggagct cgatcagcgg cgagaacgtg acgccttctc cgtaggacgg cgtctgggcg
    11581 gtcagaacgc gggcgcgccc gtcgaccgtg tccgcgaacg tctcggcgag cctcgacttg
    11641 ccgaccccgg gcggagcgag gaccgtgacg aggacgcagg actcggcttc gatcgcgtcc
    11701 tccagggcat gctcgagcga ggtgatcgcg cgcgagcggc cgaggaacgt ggtctgcgtc
    11761 agtgatggcg cttcgtagat gctcaggacg cggaagacat cgaccggttc ggtcttgccc
    11821 ttcaaggtga gaggtggcag ccgctcggtt cgcagggaac gtctcgcgag ccgctctgcc
    11881 gggcccgaga cgaggacgtc accgacgttg gcgttcgact ggagccgcgc cgcggtgttg
    11941 aacgtgtcgc cggcgacccg gccctcgatc gcggcgccgt cctccgcgaa cgtttcgccc
    12001 gcatcgatgg cgatgcgcac ccggatgcgt gcgtcgatcc cgacgtcgcg atcgagctcg
    12061 tcggcagccg ctcgcatctc caaggcagcc cggaccgcac ggagcgcatc atcctcatgc
    12121 gaggtcggga tcccgaacgc gccgacgacg gcgtcaccga tgaacttctc gacggtaccc
    12181 ccgtgccgct cgatcgacgc gcgcatcgtc ccgaagtagc ggtccatgat ccgcttcagg
    12241 acctcggggt ccgtgcgctc ggcgagttcc gtcgatccga cgaggtcgca gaagagcgcg
    12301 gctacgatcc ttcgacgggg cgtcgcttcc tgcgccgcga gtcgttcgcc gcatgcgttg
    12361 cagaacctgg ccgtgtccgg gttcgaggtg ccgcatgtct gacacgttcg cacggaaggg
    12421 agtgtcgagg acggcggcaa ctgccgcaac gtgcctcacc ggctcgagcg cagcagcaga
    12481 ccacgtcgtg acggggacaa cccaagaccc aagtgacgtg cctactacat gcccgaacgt
    12541 gctgaaccgc gggatctcac cgttccgggg ggaggagtcg aacctccata ccaggctcca
    12601 aaggcccgtg tcttgccgtt agaccacccc ggagggacct gcttcgcttc cgtgatcgat
    12661 tctggcaggc gtcggcgcgc gagggggcgc ctccaccacg tacgccccgg ggaccgatcc
    12721 cgccacgtcc tcggccaccg cgaacgaaca gagcgcgacc accgtcgggc cggatcccgt
    12781 catcacggcg ccgtgcgcgc cggtgtccag gaacgtctcg accgtcgcca cgatctcggg
    12841 atggcgtgcg gcgaccggcc cctggaggtc gttggtgagc gcgctcccga cgagggcgtc
    12901 gttgccggcc tccgccgcgg cgatcaacgc gcccgcgtcc gggcccgtcg ccggttcctc
    12961 atcccaccac gcgtaggcgt cggctgacga cacaccgaac gcgaacggct tcacgaccca
    13021 tgtggtcgac tgcacgagga ccgccgcgac ccgctcgccg cgtccgtgcg cgtacgcggg
    13081 ctccccgagg agcagggcgg gaacgtcgga tccgatctcc gctgcgagac gggagaggcc
    13141 gtcgcggccg aggcccgtcg accacagctc gtcgagcagc agcagcgtcg ccgctgcatc
    13201 ggccgacccg ccgccgaggc cggcggccac ggggatccgt ttgtcgatcg tgatgtgcgc
    13261 gcctaccgga cttgcgccgc ccacctcggc ggcgagcgcc cgcgccgccc gaagcgccag
    13321 gttcgcgtcg tccgtgggca gccccgcagc ggcggatccc tcgacggaca cggagagctc
    13381 gtcggccggc tccacccgga catggtcggc cagcgagatc ggcaggagca gcgtctcgat
    13441 gtcgtggtag ccgtcatccc gacggccgat cacgcgcagg aacacgttga ccttcgcgtg
    13501 ggcgggcatc gtcagccccc gcgtctcgga tcgcctcatc tcggtggcgc cgtgtcctcg
    13561 gggaaggact cggcgatccg cgcgaactcc tcgagtgaga gcgtctcgcc ccgggccgag
    13621 ggatcgacgc cggcggcttc cagcagctcg tccgcgacct cgccctccag accgagccgg
    13681 cggagggcgt tccgcatcgt cttccgccgc tcgccgaacg cggcatccac caccgcccag
    13741 agccggtgct cgtcgaccgc gaccgccggt tcgtcccggc gcacgagccg cacgatcacg
    13801 gactccacct tcgggcgcgg ccagaagacc gacggaggca cgcggcgctc gagggttccc
    13861 tctgcccggt aggccacacg gacgctcggg atcccgtagg cgggctcgcc cggcgccgcc
    13921 accagtcgct cgccgacctc gcgctgcacc atcacgacga ggcggcgcac ccgagggacg
    13981 tcccgaagcg tgtcgagaac gatgtgggtg gcgatgttgt acggcaggtt cgcagccagc
    14041 acccatccat cgccgtcgag ggcgtcctgc cactcaggat ccgtcgcatc cgtgtggagg
    14101 acccgcaccc ggtccagccc ggcgaccgat tcctccagcg ccgagatcag cgaaggatcg
    14161 acctccaccg cgagcacctc tgcgccggct tccgcgagcg ctcgcgtgag cgagccgagt
    14221 ccggcgccga tctcgacgca ccggtcgccc ggccccaccc cggcatccgc ggcgatcgcg
    14281 cgcgcgaggt tcgggtcgat caggaagtgc tgacccagcg agcgcttcgg tcggatgccg
    14341 tggcgttcgg cgagggcgcg cagcgcgccc gcgccgaacg aagcatgttc agcggtgctc
    14401 acgcggggag cgtaacgggg ccggaacggg ttcggtcccg ggcgaaggcc cgggaccgaa
    14461 cgtgaagggg ggcgctggct gtcgtccgga cgccgtcagc gaggggcacg atccccatgg
    14521 ccgagaggag ctccccggtc ggatccgtgg tcgtcgagcc ctcgcgcacc ttccccgaac
    14581 gcgagaaccc gcacggactc ggccgaggat gtcccgacgc cgccggcgga aaccgaggtc
    14641 tcggcggtga ggcccatgcg cttccaaacg ggcatcaccg ctccgtccac caaccagtcc
    14701 cccacacctc ggggacgatc gttcgatgac gacgtcacac gccgaacccg cggatcctgc
    14761 cgagtcgtcg tccgaacgtc gcgcagcttc cgtcccgctc cctccgagga ccggtgtccc
    14821 cgcgggacgc gagtgacctc ggaagctgtt gtggaaaacg cacgctagca aaccctcaac
    14881 tctcactgca accttgagcg tcgaacgtat gttcggccta ggagagaccg aagaacaccg
    14941 cgtcggcgtt gcgcgacgtc gcctccgaca cgcttcgcac gtcctcgtcg cggatgcggg
    15001 cgacctcggc gatcgtgtgg atgacgttcg ccggttcgtt gtccgtcccc cggagggtct
    15061 gcggggcgag gaagggcgcg tccgtctcca cgaggatgcg gtcgatgtcg acggcgcgcg
    15121 ccgcttgccg gagatgctcg ttcttcgggt acgtgatgtt gcccgcgaag ctgatccaat
    15181 acccgcgctc ggcgcactcc ttcgcgatcg cggcgtcgcc gctgaagcag tggatcacga
    15241 cgcgctccgc cgaaccctca tccaggactc gaaggacatc gggccacgcg tcccggacat
    15301 gcaccaccat gggcttg
//
LOCUS       DASUYD010000170         5984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_99502, whole genome shotgun sequence.
ACCESSION   DASUYD010000170 DASUYD010000000
VERSION     DASUYD010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99502"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VF195_09710"
     CDS             <1..212
                     /locus_tag="VF195_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013873062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581132.1"
                     /translation="LESVLHTLSEREKKVIQLRFGLLDGHPRTLEEVGREFGVTRERI
                     RQIESKTLSKLRHPSRSQKLRDYLE"
     gene            complement(238..498)
                     /locus_tag="VF195_09715"
     CDS             complement(238..498)
                     /locus_tag="VF195_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581133.1"
                     /translation="MRFKTGLVVGGVIGYIVARELTKQDPDDERPPGVRAAMARHPSN
                     RIAVQGRRIAEKAGWKSVEAIQRARANIQRRLDPDVDDISLN"
     gene            549..624
                     /locus_tag="VF195_09720"
     tRNA            549..624
                     /locus_tag="VF195_09720"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Asn,seq:gtt)
     gene            874..1053
                     /locus_tag="VF195_09725"
     CDS             874..1053
                     /locus_tag="VF195_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581134.1"
                     /translation="MQRIQVRRQGPSYRDRELRTEVLRLDPRDEDVVRVKNGGILTAR
                     FGEALAPVTRERRGR"
     gene            1050..2258
                     /locus_tag="VF195_09730"
     CDS             1050..2258
                     /locus_tag="VF195_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX6581135.1"
                     /translation="MSSPSDADRPDADVPVRPEMHIASNQALPARRHLRGPFIWFAAV
                     LAIVLVATLVSFGLGDRQRSQTNSTDAFAATARSLVQSEEAAPRSPATKTIASVLPSV
                     VNVRVVKVAASASGQVGEARAEGSGVVIAPNGVIVTNNHVVQGAVQVRVVFTDGHPTM
                     PGTVVGTDPQHDIAVVKVDASGLDPATLGRSSELALADTVYAIGFPLDLGVTVTRGIV
                     SGLDRSVAVQGESTVEHLVGLLQTDAAINPGNSGGPLVDTSGHVVGIDTAGASAASAD
                     NVGFSIAIDDALPVIKQILTQPTARQAWLGVEALSVNTAASAAALGLDPSIRGAAVVG
                     VLPSSPAAAAGIQAGDVIVSLDDRSIASAPALTEALATLAPGQDVTIELVTMSGRQTV
                     DVRLGQRPPA"
     gene            2264..2575
                     /locus_tag="VF195_09735"
     CDS             2264..2575
                     /locus_tag="VF195_09735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581136.1"
                     /translation="MQRGVVIPVMWVLQAVVVGGLVFASVAARRGAKRLERVVEDLEL
                     ERAVFLGILGIARRLVSRYEQLEALVDEVSYGRIDCGMQVVDGEGRVIGTVLIPPPVL
                     N"
     gene            2862..3191
                     /locus_tag="VF195_09740"
     CDS             2862..3191
                     /locus_tag="VF195_09740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581137.1"
                     /translation="MQIVYVMEVIDPLESPNGARYVRRFTYETREQAWARYSEAIRFG
                     LEAYVDEVGRMHVTNQTTAHEHVSRSSVAAWRAPHDSAVGERRRSGERSNPGGGDALG
                     LSGSASA"
     gene            complement(3306..3740)
                     /locus_tag="VF195_09745"
     CDS             complement(3306..3740)
                     /locus_tag="VF195_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581138.1"
                     /translation="MRRNVLAFVVTLVLGVSGVATAAVFAGTGSPSGAPPGRSDQAEA
                     NETDAGVHGGPNERFHDAASCDLTALASLPGNWTHGDYVSAVAAGANPALIRQAAHSD
                     CGKPMVSVGHGGGPPVALEHKAAGQADAGGEPDDAGTDAIGS"
     gene            complement(4456..5334)
                     /locus_tag="VF195_09750"
     CDS             complement(4456..5334)
                     /locus_tag="VF195_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581139.1"
                     /translation="MTYEIINGWIVGKLPGPNGDLIPVKHFPQGPYERRQRNKPLLCL
                     HTTETDGYVEDLRFPTEFQVGEDVVGQHRPLWARGAAVDEHDHDLLQIEIVGFSKLER
                     WLPKPSSLSPLVALMALLHRRGFVVTALRRPSRWPVPLDRLPAAVDTYYRRLEVWDHA
                     FVYGHVEIPGDEHWDPGSLDHPVLFSMVRDVLAPKEVDREMLSDNQLEGIQFANGMRR
                     YLDSPENEPAEPGPHRQGFRFAQRITTHIEGEPPPAAFEPSRSSTQGMQLSPDETHPG
                     RQEETQSRGGTEGSSD"
     gene            complement(5462..5749)
                     /locus_tag="VF195_09755"
     CDS             complement(5462..5749)
                     /locus_tag="VF195_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581140.1"
                     /translation="MTPCLAEITDDGVGFMAPGTLQSGRGHLGLSTMRERAEMAGGWC
                     HLISLRGAGTTVEIWVPGSAPPPPRHLEGSEGDIAAERETAALEFAAADDR"
     gene            5812..>5984
                     /locus_tag="VF195_09760"
     CDS             5812..>5984
                     /locus_tag="VF195_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX6581141.1"
                     /translation="MIEPIADVEDLRRHLQWAVEVEHSTIPPYEYAMWSVVDPDSAAA
                     TSIKYVAREEMLHA"
BASE COUNT          970 a         1996 c         2036 g          982 t
ORIGIN      
        1 agctcgaatc cgtgctccac acactgtcgg agcgggagaa gaaggtcatc cagctccggt
       61 tcggcctgct cgacggtcac ccccggactc tggaagaggt cggccgtgag ttcggggtca
      121 cgcgcgagcg gatccggcag atcgagtcga agaccctgtc gaagctgcgc cacccgtcac
      181 gctcccagaa gctgcgcgac tacctcgagt agcgttcggc ggcgggcgac ccgaaggtca
      241 gttcagcgag atgtcgtcga cgtcagggtc gagccgtcgc tggatgttcg ctcgagcccg
      301 ctggatggcc tcgacgctct tccagccagc tttctcggcg atgcgccgcc cctgcacggc
      361 gatccgattg gacggatgcc gggccatcgc cgcgcgaacg ccgggaggcc gctcgtcgtc
      421 cgggtcctgc ttggtgagct cgcgggcgac gatgtagccg atcactcctc ccaccacgag
      481 accggtcttg aaccgcatcc cctgagccta gtccaatcgg aggccgttgg tagactgcct
      541 cggctccatc cggggtagct caattggcag agcggcggac tgttaatccg taggttgtgg
      601 gttcgagtcc cacccccgga gccaccgtca gctacacacc tgcgcggacc cgaagaccgc
      661 ctgtctccgg agggctgagc gggccgcggg cgcggagggc cagcgcccga ccagcacgcc
      721 cggaggtcac ggcgtctcga acccctgcta ccgggacagt cgatcttgct gcctgccgac
      781 aggtcggttg ggtgctgccc ctggcgacgg ctcggtcgcc gaggagcagc atcagccgag
      841 gacagacggt cgagccgagc aaaggagaca gacatgcaac ggatccaggt ccgccggcag
      901 ggaccatcgt accgagaccg agagctgagg accgaggttc tccgtctgga tccacgtgac
      961 gaagacgtcg tgcgggtcaa gaacggaggg atcctgacgg cgcgattcgg cgaagccctc
     1021 gcgccggtca cgcgagagcg gaggggtcga tgagcagccc atcagacgcc gatcgcccgg
     1081 acgccgatgt ccccgtgcga cccgagatgc acatcgcgtc gaaccaagcg ttgcccgcga
     1141 gacgacacct ccgtgggccc ttcatatggt tcgccgccgt actcgcgatc gtcctcgtgg
     1201 cgacgctcgt ctcgttcggg ttgggggatc ggcagcgttc gcagacgaac tcgaccgatg
     1261 cgttcgcggc gaccgctcga tccctcgtgc agagcgagga ggcggcgccg cgatcgcccg
     1321 ccacgaagac gatcgcctcg gtcctgccct ccgtcgtcaa cgtgcgcgtc gtgaaggtcg
     1381 ccgcgtcggc gagcggccag gtcggggaag ccagggccga ggggagcggc gtcgtgatcg
     1441 cgccgaacgg cgtgatcgtg accaacaatc acgtcgttca gggggccgtc caggttcgcg
     1501 tcgtgtttac ggacggtcat ccgaccatgc ccggaaccgt cgtcggaacg gacccacagc
     1561 acgatatcgc cgtcgtcaag gtcgacgcct ccgggctcga cccggcgacg ctggggcgat
     1621 cgagcgagct cgcgctcgcc gacaccgtct acgcgatcgg attccccctc gacctgggtg
     1681 tgacggtgac acgcggcatc gtctccggtc tggaccggag cgtggcggtt cagggtgaat
     1741 cgaccgtcga acacctcgtc ggcctgttgc agaccgacgc ggcgatcaac ccggggaact
     1801 cgggcggtcc gctcgtcgac acatccgggc acgtcgtcgg catcgacacc gcaggcgcct
     1861 ccgcggcgtc ggccgacaac gtcggattct cgatcgcgat cgacgacgcc cttcccgtga
     1921 tcaagcagat cctgacacaa ccgacggcgc ggcaggcgtg gctcggagta gaggccttgt
     1981 cggtgaacac cgcggcgagc gcggccgcgc tcggactcga tccgtccatt cgcggcgccg
     2041 ccgtcgtcgg cgtgctgcca tcgagcccgg ccgccgccgc cgggatccag gctggtgacg
     2101 tgatcgtgtc gctggacgac aggtcgatcg cgtcggcgcc cgctctcacc gaggcgctcg
     2161 ccacgctcgc gcccggtcag gacgtcacga tcgagctcgt caccatgtcc ggtcggcaaa
     2221 ccgtcgacgt tcgcctcggc cagcgccccc cggcgtgagg cccgtgcaga gaggggtggt
     2281 gatcccggtg atgtgggtgc tgcaagcagt cgtcgtcggt gggctggtct tcgcgagcgt
     2341 cgctgctcgc cgaggggcca agaggctgga gcgggtggtc gaggacctcg agctcgagcg
     2401 ggccgtcttc ctcggcatcc tcggcatcgc tcgccgcctc gtctcgaggt atgagcagct
     2461 cgaggcgctc gtcgacgagg tttcctacgg ccgcatcgat tgtgggatgc aggtcgtcga
     2521 cggtgagggg cgcgtcatcg ggaccgtcct catcccgccc cccgtactca actgagctcg
     2581 cccgtgatgc aggagaggac gaggaccatg tggaatcacg agtcgttggc tcccaccaca
     2641 actcctggtc gacgcggccc ctctcttgag acggccccac acactcttgg gggacctcct
     2701 catcgggcaa acccacggga agattcgggc tttcgacgca ttcaccccaa ctccgaccct
     2761 gtcgatattg acgtcgcggg ccggcaacga ggccgcccgt ggacatcact ccggaggcgc
     2821 tgtgtccagc ctgatcccga cacgcagctg acgaggacga gatgcagatc gtctacgtga
     2881 tggaggtcat cgaccctctc gagagcccca atggggcgag gtacgtccga cgcttcacgt
     2941 atgagacgcg cgagcaagcg tgggcgaggt acagcgaggc gatccggttc ggcttggagg
     3001 catacgtcga cgaggtcggt cgaatgcatg tcacgaacca gacgactgcg cacgaacacg
     3061 tcagcagatc atcggtggcg gcttggcgcg cgccgcacga tagcgccgtc ggcgaacgac
     3121 gccgcagcgg cgagcgctcg aaccccggcg gcggcgatgc cctcggcctc tcgggatcgg
     3181 cgagcgcgta gccgtcactg ctgaccccat cgcgactgtc gaccgcgatc gcgctcggat
     3241 tcctggacct cgaacgtggc gcggccaagg ggatctgcgc tcccgctggg cctcgccagc
     3301 aacggctagc tgccgatagc gtccgtaccc gcgtcgtcgg gctcgccgcc cgcgtccgcc
     3361 tggccggccg ccttgtgctc gagcgcgacc ggtggcccgc cgccgtggcc gacagagacc
     3421 atcggcttgc cgcaatcgct gtgggcggct tggcggatca aggccgggtt agcgcctgcg
     3481 gcgacggcac tcacgtagtc gccgtgcgtc cagttgcccg ggagcgaggc caaggccgtc
     3541 agatcgcacg acgcggcgtc gtggaaccgt tcgttcggcc caccgtggac gccggcatcc
     3601 gtctcgttcg cctcggcctg gtccgaccga ccgggaggtg cgcctgaggg cgacccggtg
     3661 cccgcgaaga cagcggccgt ggcgacgccc gacacaccca ggacgagggt gaccacgaac
     3721 gctagaacgt ttcgtctcac gcgagcctcc ttcgacgctt cggacaagcg tgggtcaaac
     3781 cgagcgtcgg gtcaatcgac tgatcggcca tggccaggac cggacgacgt aggccgctgc
     3841 gcggatgtgc gagacgacca tcgtgttgag ggacgaagcc tgagccgatc gggcgatcgc
     3901 agctaggcag cttgcaaagc ccgatggcga ccggcggaag ccggtcctgg tgcggggcgc
     3961 cgacgcttca aggggtcgta gcaccgggcc gggtagccgt cgtttcgacg tcgtcgcgcc
     4021 tggtcgagat cgacgacaat cacgacaagg gctccaggag catgacacga cggtgctgtt
     4081 cgggatcgtg gtgacaccag tcgtcgggat ccacggaccc acccaactgc cccgcgttga
     4141 atctgagggt tgtggcgtgg gccgtgttcg ggtgggtctc cacgatgtgg cgagtcagga
     4201 tgcagagcac gacgctgtcg tcgggcccag gaccgatcca ccccacccag cagaccgggc
     4261 acctggcggt ggcatgtggc agaggacatg ggacgtcgtc gcttatgggg cctgaagctt
     4321 cgccgtgtac ccggcaaagg gcgtcgtctt gagagtcctt cggttctccc atctccctcc
     4381 tactcatcgc catatcgtgc ggtgaggttg agcgaggtgc tgctcgttgt ccgcgccgca
     4441 cggagtggcc gcgtgctagt cagaggaccc ttcggtgccc ccgcggctct gggtctcctc
     4501 ctgccgtccg gggtgcgtct cgtcgggtga cagctgcatg ccctgggtcg acgatcggct
     4561 aggttcgaac gccgctggcg ggggctcacc ctcgatgtgc gtcgtgatcc gctgcgcgaa
     4621 ccggaatccc tgacggtgcg ggccgggttc tgcaggctcg ttctccgggc tgtccaggta
     4681 ccggcgcatc ccgttcgcga actggatacc ctcgagctgg ttgtcggaca gcatctctct
     4741 atcgacctcc ttcggggcga gaacgtcgcg gaccatcgag aacaacaccg gatggtcgag
     4801 cgaacccggg tcccaatgct cgtcgcccgg gatctccacg tgaccgtaga cgaacgcatg
     4861 gtcccagact tccaagcgac ggtagtaggt gtccacggca gccgggagtc gatcgagtgg
     4921 gaccggccac cgcgaaggac gtcgcaacgc ggttacgacg aagccgcggc gatggaggag
     4981 cgccatgagc gcgacgagag gggataggct cgatggtttc ggcagccatc gctcgagctt
     5041 cgagaacccc acgatctcga tctggagaag atcatggtcg tgttcgtcga cggccgcgcc
     5101 tcgcgcccac agcggtcggt gctggccgac gacgtcctcc ccgacctgga actcggtcgg
     5161 gaagcggaga tcctccacgt aaccgtccgt ctcggtcgtg tgcaggcaga gcaggggctt
     5221 gttccgttgg cgccgctcgt acggcccctg agggaagtgt ttgaccggga tcaggtctcc
     5281 gttcggcccc gggagcttcc cgacgatcca gccgttgatg atctcgtacg tcatcgatgt
     5341 aggaagcgtg cgggatccac ctgcggtcgt ggttccagag gtggcgatcc cctcgattcc
     5401 accggttcat ggtagttcgt aattccgacg cgcagcccga tccctctggt cgatctttcg
     5461 tctagcgatc gtcagcggcc gcgaattcga gggctgcagt ctcgcgctcg gcggcgatgt
     5521 cgccttcgct tccctcgaga tgcctcggcg gtggtggtgc ggatccgggg acccagatct
     5581 ccacggtcgt gccggcgccg cgcagactga tcaggtggca ccaaccgcct gccatctcgg
     5641 cccgctcgcg catcgtcgaa agcccgagat gacctcggcc cgactggagg gtccccggcg
     5701 ccataaaccc cacaccatcg tcggtgatct cggcgagaca tggggtcaag gtggattatc
     5761 ccaccagccc gccgcagatc tcgacgcgat agcatccggg aaccgggaac catgatcgaa
     5821 ccgatcgccg acgtcgagga cctgcgccgc cacctccagt gggcggtcga ggtcgagcac
     5881 agcacgatcc cgccctacga gtacgcgatg tggagcgtcg tggatcccga ttccgcggcc
     5941 gccacctcga tcaagtacgt ggctcgcgag gagatgctgc acgc
//
LOCUS       DASUYD010000171         3947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_985276, whole genome shotgun sequence.
ACCESSION   DASUYD010000171 DASUYD010000000
VERSION     DASUYD010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985276"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF195_09765"
     CDS             <1..247
                     /locus_tag="VF195_09765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581142.1"
                     /translation="PDGTKLASVGLDGILRVYVLPMDELVRLARGRVTRGFTERECRQ
                     YLHLAACTAALARPPRPSVDSGSSAATDPVGPEGPIA"
     gene            complement(644..2227)
                     /locus_tag="VF195_09770"
     CDS             complement(644..2227)
                     /locus_tag="VF195_09770"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581143.1"
                     /translation="MSIERSSSADGLDSAQVTVSEEPLPRGLTRRELDVLTLLGGGLN
                     NKDIAARLKTSVRTVSTHVEHLLTKLSQRSRAGAGAVAVDQGLVRLPIPGGGQGLEGL
                     TVGLLDRAIADDRRVASAPLAEPLRPRGAARRPFLIGSALPLTGPAKGDGLEMRNGAA
                     LAIAEINARGGVAGRRLELVVVPVDVFDAASIRSAFETLTAADVDAITSLYVFAEDVA
                     IERAADYGAPYLHAMTSEHLAQSVAEDPVRYGNVFQVCPSEVHYGRGFVRFIDDLTAR
                     GLWRPQQRSVLFIETVLQSSRMATAETLEAAEASGWRIGGVHYVPAEGTDWGSVVELI
                     HRTDPGAIMVTDFLPAELAGFQRRYAEAPTDALVFAVYSPSVPEFLESARGAAEGLVW
                     STVTGTYGDEMGDRFTRHFGGFHGRPPGRSHAGIAYDEIHLLARAWSDVDNPRDFRAV
                     SDRLRRVPFRGVNGAYFLDNERQSGLAYPDMTPDPSLSQAHLVLQIQEGRHRILSPAP
                     YVEAGFRPPSWWATERASA"
     gene            2606..3943
                     /locus_tag="VF195_09775"
     CDS             2606..3943
                     /locus_tag="VF195_09775"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581144.1"
                     /translation="MGQREGGGRITRRQLLTRAGAAAVGGLVVGGVGGYVIGSSGDDD
                     GGSGGATGGGGGGGGEGTIKIGSASPTSGPYSGDGQEMTRGQELAIEDINANGGVLGR
                     QLEIVVADVEDLAPEKMVNAARRLTNEGVAATFSGYTSTTSAEFDVYAQYGAPMFHLN
                     TFQPNADYVADNQIPNIYHSCPTEVWYGPGFILFMQRLIDAGAWTPSSESAAVITSND
                     PYSISIAQTVEDELRNLGWEIVVSEEVTAPLTEWGPVLARVRQNPPGLIFHSDYIPGD
                     LASFQQQFRSDPTPSLMYQQYGPSIPEYLELAGTDADGVIWSTVIGTLPDEIGTAFKD
                     AYREKYDAEAGLSQAGAQYDLVHLWAQSAGIAGNETDFDAVNANVKRSTYRGVSGTYR
                     YKPGELTAIPYPDEVNDPSLGMPHLTFQIQSQEQVLVDPIPYDQGEFQLPAWL"
BASE COUNT          689 a         1318 c         1348 g          592 t
ORIGIN      
        1 ccccgacggg acgaagctgg caagcgtggg actcgacggc atcctgcggg tctacgtgct
       61 cccgatggat gagctcgtgc ggctcgcccg tggccgcgtg acacggggat tcaccgagcg
      121 ggaatgccgc cagtacctgc acctggccgc ctgcaccgcg gcgctcgctc gacccccaag
      181 accctccgtg gactccggtt cgtcggcggc gaccgatccg gtggggcctg aggggcctat
      241 cgcttgacga tcgaccccgc tgatctccaa caccacccgc cattctcgga ctcggacacc
      301 gagatgtacg tgggcgagta cacgctcagc cttgtgggag gtaggtgagt acctaccagg
      361 aagggccgaa gggcggaatg tacgacacct ccggcaccta cgccgtcgac ggcggcacga
      421 tcaacgttca cagaccgata cgacctcggt ggttcggcac gtcgtggagg ggccggtgga
      481 cgctcgaagc agtggagctg tcgttcaatc cgcgtgcaca agtagaggtg tgcgtgaggt
      541 tcgccacggg ccaccgacac atggtccgcc tgtccgtcgg tcggttagcg agtgggcgac
      601 tcgtcgtgaa cccatcgctc gcaccgcggc ctgcgacatg ggctcaggca ctagcgcgtt
      661 cggtcgccca ccatgacggc ggacgaaatc ccgcctcgac gtacggcgcg gggctcaaga
      721 tccggtgcct cccctcctgg atctgcagca cgaggtgcgc ctgactgagg gatgggtcgg
      781 gagtcatgtc cgggtacgcc agcccgctct gtcgctcgtt gtcaagaaag taggcgccat
      841 ttactccccg gaacgggacg cgccggaggc gatctgacac ggcgcggaag tcgcggggat
      901 tgtcgacatc ggaccacgcg cgcgccaaca ggtggatctc gtcataggcg atccctgcat
      961 gcgagcgccc cggaggccgg ccgtggaatc cgccgaagtg acgcgtgaat cgatcgccca
     1021 tctcgtcgcc gtacgtcccc gtcactgtgg accacacgag gccttcggca gcgccgcgtg
     1081 cgctctccag gaactccgga acggacgggg agtagacggc gaagacgagc gcatctgtcg
     1141 gcgcttccgc gtaccgacgc tgaaaccccg cgagctcggc ggggaggaag tcggtgacca
     1201 tgatcgcgcc gggatcagtg cggtggatca gctcgacgac cgatccccag tcggtaccct
     1261 ccgcggggac gtaatggacg ccgccgatgc gccacccgga cgcttctgcg gcctcgagcg
     1321 tctccgccgt cgccatgcgg ctgctctgca gcaccgtctc aatgaacagt accgagcgct
     1381 gctgcggacg ccacaggcct cgagcggtga ggtcgtctat gaagcgcacg aagccgcggc
     1441 cgtagtgaac ctccgagggg cagacctgga agacgttgcc gtagcgcacg gggtcctcgg
     1501 cgaccgattg ggcgagatgc tccgacgtca tcgcatggag atacggggcg ccgtagtcgg
     1561 cggcgcgctc gatcgccaca tcctccgcga agacgtacaa gctcgtgatc gcgtcgacgt
     1621 cggcagcggt cagggtctcg aaggcagagc ggatgctcgc cgcgtcgaag acgtcgaccg
     1681 ggacgaccac gagctcgagc cgccggccgg cgacgccgcc ccgcgcgttg atctcggcga
     1741 tcgcgagggc cgcaccattg cgcatctcca ggccgtcgcc tttcgccggt ccggtgagcg
     1801 gcagcgcgga tccgatcagg aagggccgcc gagccgctcc gcgcggtcgc agcggctcgg
     1861 cgagcggtgc tgatgccacc ctccgatcgt ccgcgatcgc cctgtcgagc aagccgaccg
     1921 tgagtccttc gagaccctgg ccaccgccag ggatcgggag acggaccagg ccctgatcaa
     1981 ccgcgaccgc ccctgcgccg gcccgcgatc gctgcgacag cttcgtcagc aggtgctcca
     2041 cgtgcgtcga caccgtgcga acgctcgtct tcagccgcgc ggcgatgtcc ttattgttga
     2101 gaccaccgcc gagcagtgtg aggacgtcga gctcacgacg ggtcaagcca cgcggaagcg
     2161 gctcctcgga cactgtcacc tgggcggagt cgagaccgtc ggcgctgctc gaacgctcga
     2221 tggacacgcg gtaccacgcg tcgtcctggc gccagaagaa gtgagcccga agtcgatcgg
     2281 tcagtaggag cgcgatcgcg tacgccgtga tctccgggcg cccctcgagc catcgtcgcc
     2341 ccgcggcacc gtcgacctca tcgagtgtgc cgtccgcgtg aacccgggcg gatggtgggt
     2401 acgtgttcgt ggacgtcgtg gaccctccga tggggtcggg ccttcaccga atcgtgccag
     2461 acccggtcct gagccgtcca ggacgcactc ggacgcaacc cctcgcgtcg gtcagatgac
     2521 ggatgtggtg ccacgggctc ccttcgtatc gtggctgagc gcggccttga gccgcgtact
     2581 cacctgcacg aggaggaggg ggctgatggg gcagcgcgaa ggcggcggaa ggatcacgcg
     2641 acggcagctc ctcacgcgag ccggcgcggc cgcggtcgga gggctcgtcg ttgggggcgt
     2701 cggcgggtac gtcatcggct cctcgggcga tgacgacggc ggcagcggcg gcgcgacagg
     2761 aggaggcgga ggcggaggcg gcgagggcac catcaagatc ggctccgcaa gtccgacgag
     2821 cggaccgtac tcgggcgacg gccaggagat gacgcggggg caagagctcg cgatcgagga
     2881 catcaacgcc aacggcggcg tcctcggccg gcagctcgag atcgtggtcg ctgacgtgga
     2941 ggacctcgcg cccgagaaga tggtcaacgc cgcgcggcgg ctcacgaacg agggcgtcgc
     3001 ggcgaccttc tcgggttaca cctccacgac gtcggccgag tttgacgtat acgcgcagta
     3061 cggcgcgccc atgttccacc tgaacacgtt ccaaccgaac gccgactacg tcgcggacaa
     3121 ccagatcccg aacatctacc actcgtgtcc gaccgaggtg tggtacggac cggggttcat
     3181 cctgttcatg cagcggctca tcgacgccgg agcatggacg ccgagctcgg agagcgccgc
     3241 ggtcatcacg agcaacgacc cgtacagcat ctcgatcgcc caaaccgtcg aggacgaact
     3301 ccggaacctg ggatgggaaa tcgtcgtgtc ggaggaagtt acagcgcccc tgaccgagtg
     3361 gggacctgtc ctcgcgaggg tccgccagaa cccgccgggc cttattttcc actctgacta
     3421 catccccggc gacctcgcct cgttccagca gcagttccga tccgacccga cgccctcgct
     3481 gatgtaccag cagtacggcc catcgatccc cgagtacctc gagctcgcgg gcaccgacgc
     3541 cgacggcgtc atctggtcga ccgtgatcgg gacgctgccc gatgagatag gaacggcctt
     3601 caaggacgcg taccgcgaga agtacgatgc cgaagcgggt ttgagccagg cgggcgccca
     3661 atacgacctc gtgcacctgt gggcgcagtc cgccgggatc gctgggaacg agacggactt
     3721 cgatgccgtg aacgccaacg tcaagcggtc cacgtaccgg ggggtcagcg gcacgtaccg
     3781 atacaagccc ggcgagctga cggcgatccc ctatccggac gaagtgaatg atccgagcct
     3841 cgggatgccg catctgacgt tccagatcca gagccaggag caggtgctgg tcgacccgat
     3901 cccatacgac cagggcgagt tccagctgcc cgcgtggctg tagaggt
//
LOCUS       DASUYD010000172         5828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_324322, whole genome shotgun sequence.
ACCESSION   DASUYD010000172 DASUYD010000000
VERSION     DASUYD010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324322"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VF195_09780"
     CDS             <1..610
                     /locus_tag="VF195_09780"
                     /inference="COORDINATES: protein motif:HMM:NF025109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HEX6581145.1"
                     /translation="ELSGMSEKSATEAVAGWLERLHLGDRADARLEELSHGNQQRVQL
                     AAAIVHSPELAVLDEPFSGLDPLGVESLAELLKQIAAMGVAVVFSSHQLDLVEDVCED
                     VVIIDHGRIVMTGVVEELKAASPHRSLEVTVNGEPWVPILPGGAVTTKPGARLRLLVD
                     ATADVEEILATARQAGEVTSFTFEPPSLTDLFLDVISEGRAA"
     gene            607..1794
                     /locus_tag="VF195_09785"
     CDS             607..1794
                     /locus_tag="VF195_09785"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581146.1"
                     /translation="MNGVLQRVWLVTRREWNQRARTRAFRISTLISIGIVVVLIMAPE
                     IYGGGDGNRRTIGLVGASSAQLPEVLEASGDQLGLSVKTRRFADEPAGRVALRSGDVS
                     VLLVEQARLVWEADPDEQLQGIVISAVGTLERQRAIEDLGLTSEEAQRLLRAPELRST
                     SLERATEEQTARADLGRIGVVLLFVAIAFYCGFVLVGVVEEKSSRVVEVLLSRVRPTE
                     LFAGKILGIGLVGLAQFALVVVSALVALSVADNTLAPDTTPSTLGWIVFWFVLGYAFY
                     AVLYAAAGSLVSRQEETQSLQLPMTGVLFVAYILAFVATESPDGAAALMGSFIPPTAP
                     MVMIVRIAHGAVPWWQIVLSVGLMVATIYGIVQVAGRIYAGGVLRFGGRVRLKEAWRG
                     AES"
     gene            2124..2753
                     /locus_tag="VF195_09790"
     CDS             2124..2753
                     /locus_tag="VF195_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581147.1"
                     /translation="MTRTGTVQELAAPLGSVDVGQLVFTRGAAHLTIGVDGSMDDLYR
                     ARFEGKVPDIRVDGGTITVKYRIGLRPPRGEIVLSGRVPWAIRARMGMSHVVADLEGL
                     ELRGLEISAGASHVEVRLPRPEDTTRVRIGGGASDVELIRPVGVPVRVHIGGGVSTLA
                     IDEFRLGSAGSKTDWRSPNYEQVAGRYDVEIGAGASNVIIRTSEQVVDR"
     gene            complement(2974..3816)
                     /locus_tag="VF195_09795"
     CDS             complement(2974..3816)
                     /locus_tag="VF195_09795"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX6581148.1"
                     /translation="MSRSISLTVAVLVGGLLMSSCGADSGSEDGGFHTTSEVVSNEAT
                     KDMLVFAPDAEGRWPVVVAFHGIGGTGQDMAEIATRLAREGSVVFAPTYGTDITTQEG
                     IDRAAVDAECDYRFARSIAAEYGGDLDQPVTFVGWSLGASAALGIGLTEEIDPTGEFV
                     SCFGQVPRPDIIVAMSGCHYEGGQLDLVDTEAWGNKEAEILLLAGEKDTNCPPWETED
                     AAAEMRSAGYEVNLLMLDGADHFAPVFHDLQDGQFVVVANDPAGERTVELILDAIAAK
                     QHRT"
     gene            complement(3888..4343)
                     /locus_tag="VF195_09800"
     CDS             complement(3888..4343)
                     /locus_tag="VF195_09800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6581149.1"
                     /translation="MSVRSPEREIAPDRAAASAPADVARIPGKYLSITSFRRNGSGVA
                     TPVWFATEGDRLFVMTALGSGKVRRIRRNPFVSIAACSARGRLRSEPMTARAEILPPT
                     KEEHVKQLIARKYRFDLLFIRPIRALQGLFHPERRHEAAAVLAITPVSS"
     gene            4857..5573
                     /locus_tag="VF195_09805"
     CDS             4857..5573
                     /locus_tag="VF195_09805"
                     /inference="COORDINATES: protein motif:HMM:NF042511.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HEX6581150.1"
                     /translation="MHDHVSGIEVPAPVGRYIETAYADGVPGVGTASMEGSGRFRQRP
                     LPWLPFANTIWLRPGVDRVSDMFVRIGPITVMKVLDAYVDGRGITKFLSRTDMGPRVD
                     QGAMHPMLCESLMFPSCWSRTPGFAWEPVDADTARVHMPFEAGFEVASVGFDPDTGFP
                     ATFETMRFKGKDGPKVRWRITMLGWRRFGEVTIPERIMVRWADEPGPWLKLRFERVTI
                     GVDLDEPLTRARNAIATASA"
     gene            complement(5588..>5828)
                     /locus_tag="VF195_09810"
     CDS             complement(5588..>5828)
                     /locus_tag="VF195_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581151.1"
                     /translation="VFLGTVHAGHPGGMLPLTEASARTMHDDRLPENAYVADATLFPK
                     SLGNPPILTIIAMAKRIAAICAARVGPAHLARSRR"
BASE COUNT          885 a         1873 c         2089 g          981 t
ORIGIN      
        1 cgagctgtcc gggatgagtg agaagtcggc gacggaggct gtcgccggct ggctcgagcg
       61 cctccacctg ggcgaccgag cggacgcgcg tctggaggag ctctcccacg ggaaccagca
      121 gcgcgtgcag ttggccgccg cgatcgtgca cagcccggag ctcgcggtgc tggacgagcc
      181 gttcagcggg ctggatccgc tcggggtcga gtcgctcgcc gagctgctga agcagatcgc
      241 cgcgatgggg gtcgccgtgg tgttctcgag ccaccagctc gatctggtgg aggatgtgtg
      301 cgaggacgtc gtgatcatcg accacgggag gatcgtgatg acgggtgtgg tcgaggaatt
      361 gaaggccgca tcgccccacc ggtcgctgga ggtgaccgtg aacggcgagc cgtgggtgcc
      421 gatcctgccc ggcggcgccg tcaccaccaa gccgggcgcc cggttgcgcc tgctggtcga
      481 cgcgacggcc gacgtggagg agatcctcgc gaccgcgcgc caggcgggcg aggtgacgag
      541 cttcaccttc gagccgccct cgctcaccga cctgttcctc gatgtgattt cggaaggacg
      601 ggccgcatga acggcgtgct gcaacgcgta tggcttgtga cccgtcgcga gtggaaccag
      661 cgagcccgaa cgcgggcgtt tcggatctcg acgctgatct ccatcgggat cgtcgtagtc
      721 ctgatcatgg cgcccgagat ctatggggga ggcgacggaa acaggcggac gattggcctc
      781 gtcggcgcga gctcggcgca gctcccggag gtcctggagg cctcgggtga ccagctcggc
      841 ctgtcggtca agacccgcag gttcgccgac gagcccgccg gtcgggtcgc cctgcgatcg
      901 ggggatgtgt cggtcctgct ggttgagcag gcgcggctcg tgtgggaggc ggatcccgac
      961 gagcaactcc agggcatcgt gatctctgcg gtcgggacgt tggagagaca gcgggcgatc
     1021 gaggatctcg gtctcacctc cgaggaggcg cagcggctat tgcgggctcc ggagctgcgg
     1081 tcgacgagcc tcgaacgggc caccgaggag cagacggcac gtgcggatct gggccggatc
     1141 ggtgtggtgc tgctgttcgt agcgatcgcg ttctactgcg gcttcgtcct ggtcggcgtc
     1201 gtggaggaga agtccagccg cgtggtcgag gtgcttctgt ctcgggttcg gccgacagag
     1261 ctgttcgccg gcaagatcct cgggatcggc ctcgtgggcc tcgcgcagtt cgccctggtc
     1321 gtggtgtcgg ccctcgtcgc gctgtcggtc gcggacaaca ccctggcgcc ggacacgacg
     1381 ccgagcacgc tcgggtggat cgtgttctgg ttcgtcctcg ggtacgcgtt ctacgcggtc
     1441 ctgtacgcag cggccggctc gctggtctcg cggcaggagg agacccagag cctccagctt
     1501 ccgatgacag gggtcctgtt tgtcgcgtac atcctggcgt tcgtggcaac cgagtcgccg
     1561 gacggcgccg cggcgctcat ggggtccttc atcccaccca cggctccgat ggtgatgatc
     1621 gtccgtatcg cgcatggcgc cgtcccgtgg tggcagatcg tcctctcggt tgggctgatg
     1681 gtggcgacga tctacggcat cgtgcaggtg gccggccgca tctacgccgg gggcgtgctt
     1741 cggttcggcg gccgggttcg gttgaaggag gcctggcgcg gcgccgagtc gtgacgttcg
     1801 gcggtgctgg aggaacgaac tggcgtcaaa cgctgggccc cccaggttgc gacaagatgg
     1861 tctgtgctcc cctcaacgga cacaggacgt ctagctcccg aacgttctct ggagagagaa
     1921 gggtcgcaat cgacaggtgt acgccgaccg ggtgtgaggg tgacctgaag aagtcgtgac
     1981 ctcgccccga tcccacaagc aactccattc ctcgctcgtg ggtcccgaaa cgagagtgac
     2041 catcggccct gagggcactc cgccacggtg cgtagcgtgg catcgagcca tccgggcatc
     2101 gcgtgagagg agatgagatt gagatgacca gaaccggaac cgtgcaggag ctggccgccc
     2161 cactgggttc ggtggacgtc gggcaactgg tgttcacgcg aggcgccgcc cacctcacca
     2221 tcggggtgga cgggtccatg gacgacctct atcgggcgcg gttcgagggc aaggtgccgg
     2281 acatccgggt cgacggaggc acgattaccg tcaagtaccg cattggcctg cgccccccgc
     2341 gcggcgagat cgtcctgtcc ggccgggttc cctgggcgat ccgggcccgc atggggatgt
     2401 cccacgtcgt ggccgacctc gagggcctgg aactcagagg gctggagatc tccgcagggg
     2461 cctcccatgt cgaggtgagg ctgcctcgcc ccgaggacac gacgcgcgtt cggatcgggg
     2521 gcggagccag cgacgtcgag ctgatccggc cagtcggtgt gcccgttcgc gtccacatcg
     2581 ggggtggggt gtcgacgctc gccatcgacg agttccggct cgggtccgcc ggcagcaaga
     2641 ccgactggcg gagccccaac tacgagcagg ttgcaggtcg gtacgacgtc gagatcggcg
     2701 ccggagccag taacgtcatc atccgaacct cagaacaggt cgtggatcgt tgacctcctc
     2761 cggctggatc gccggctcgc cgcgcggcgc ggggctcccg gtccgcccag tgacccagtc
     2821 gtgatcacga gaatcgttga ctcagtggac gtcgagactg cacttcgagc agttgagcga
     2881 caggttccgg agtcgcgttc gaggtcgggt tttcaggttc agtagccccg gccggctcgt
     2941 agtgcggcgc tggccccggt gcgaggtgtc caatcaggtt cggtgctgct tggcggcgat
     3001 ggcatccagg atcagctcga cggtccgctc ccccgcagga tcgttcgcga ccacaacgaa
     3061 ctggccgtcc tgaaggtcgt ggaagaccgg ggcgaaatgg tccgctccgt cgagcatcag
     3121 gagattgact tcgtacccgg ccgaccgcat ctcggcggcg gcgtcctcgg tctcccacgg
     3181 agggcagttg gtgtccttct cgccggcgag gaggaggatt tcggcctcct tgttccccca
     3241 cgcctcggtg tcgacgaggt ccagctgccc gccctcgtag tggcagccgg acatggccac
     3301 gatgatgtcc ggacgaggca cctggccgaa gcacgagacg aactctcctg tcgggtcgat
     3361 ctcctcggtc agaccgatcc ccagggccgc gctagctccc agggaccatc cgacgaaggt
     3421 cactggctga tcgaggtcac cgccgtactc ggccgcgatg ctgcgagcga agcggtagtc
     3481 gcattcggca tcgaccgctg cccggtcgat gccttcttgg gtggtgatgt cggtcccgta
     3541 ggtcggggcg aacaccacgg acccttcgcg agcgagacgg gtcgcgatct cggccatgtc
     3601 ctgaccggtc cccccgatcc cgtggaaggc caccacgaca ggccaccgcc cttcggcatc
     3661 gggtgcgaac accagcatgt ccttcgtagc ctcgttcgac acgacctcgg acgttgtgtg
     3721 gaacccgccg tcctccgagc ctgagtccgc cccgcacgaa gacatcagca gaccaccgac
     3781 cagaacagca acggtaagag agatcgaccg actcatccat gcctcctcgt ggccgacctt
     3841 ttgcccctag cgtgcccgac aactgatcaa cccggagccg agcatcgtca ggacgagact
     3901 ggggtgatcg cgagcacggc ggccgcctcg tgtcgacgtt ccggatggaa caggccttgc
     3961 agcgcgcgga tcggcctgat gaagagcaga tcgaaccggt acttgcgcgc gatgagttgc
     4021 ttgacgtgct cctccttggt cggcggcagg atctcggccc gggccgtcat cggctcacta
     4081 cggagccgac cgcgggccga gcacgcggca atgctcacga acgggttccg gcggatccgc
     4141 ctgaccttgc cggatccgag cgccgtcatg acgaagagcc ggtcaccctc ggtggcgaac
     4201 cacaccgggg tcgcgacgcc cgagccgttg cggcggaagc tcgtgatgct caggtacttg
     4261 cccgggatcc gcgcgacgtc ggccggcgct gacgcggcgg ctcgatcagg cgcgatctcc
     4321 ctctcggggc tgcgaacaga catcgtgctc acctcccgac cggccgggac tgccgtccgg
     4381 tcacccctca tcgtcggcga gcgccgccgc cgcgacctgg gtcggacgac ccggttccga
     4441 taggccatcg ggatctggtc ggacgctcga gcgaaacccg atccccggag ttcggggtat
     4501 tgccggttgc cggaggggcg gtcgcgcggt tcggggagaa ccactgcgcc cgccgctccc
     4561 cgagcgagag caagttgaca gcccggaata cccgcggtgg ctcctggagc agggcctggc
     4621 cgcagggcgg ccgttgtgag ctcgctgccg ccgggccgtt cgggatggct tctcaggacc
     4681 gaccgggcct agcggcggag tggtgtctgg agcaggttag gtggcggagg cagggctgcg
     4741 tgaccccgta gcccgctcgg ccgaaaccgt ccggggctgt ggcctcgggt gggagtgcct
     4801 ttatggtcta cgaggtgtta accctcgccg actgaacggg gcgggaggtg gtctcgatgc
     4861 atgatcacgt gtccgggatc gaggtaccgg cgccagtcgg ccggtatatc gagacggcgt
     4921 acgcggacgg tgtccctggg gtaggcaccg cctcgatgga gggctccggc cgcttccggc
     4981 aacgcccgct cccgtggctc ccgttcgcga acaccatctg gctccggcct ggcgtggacc
     5041 gcgtctcgga catgttcgtc cgaatcgggc cgatcacggt gatgaaggtg ctcgacgcgt
     5101 acgtcgatgg tcggggcatc acgaagttcc tgagcagaac ggacatgggc ccgcgggtcg
     5161 accagggggc gatgcacccg atgctatgcg agtcgctcat gttcccctcg tgctggtcgc
     5221 ggacgccggg tttcgcgtgg gaacccgtgg acgcggacac ggcgcgcgtg cacatgccgt
     5281 tcgaagccgg gttcgaggtc gcctcggtgg ggttcgaccc ggacacgggg ttcccggcaa
     5341 ccttcgagac gatgcgcttc aagggcaagg acggacccaa ggttcgctgg cggatcacga
     5401 tgctcgggtg gcgacggttc ggagaggtca cgatccccga gcgcatcatg gtccgctggg
     5461 ccgacgagcc cggcccgtgg ctcaagctgc gattcgagcg ggtcaccatc ggggtcgacc
     5521 tcgacgaacc cctgacgcgc gcacgcaacg cgatcgccac tgcgagcgcg tagtcggcgg
     5581 cgcgccctta ccgtctcgac cttgccaggt gtgccggccc gactcgggca gcgcagatcg
     5641 cggcgatccg cttcgccatc gcgatgatcg tgaggatcgg cgggttgccg agcgacttcg
     5701 ggaagagcgt ggcgtcggcg acgtaggcgt tctcgggcag tcggtcgtcg tgcatcgttc
     5761 gcgcggacgc ctcggtgagc gggagcatgc cgcccgggtg acccgcgtgc accgtcccga
     5821 ggaacacc
//
LOCUS       DASUYD010000173         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1645938, whole genome shotgun sequence.
ACCESSION   DASUYD010000173 DASUYD010000000
VERSION     DASUYD010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1645938"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF195_09815"
     CDS             complement(<1..186)
                     /locus_tag="VF195_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581152.1"
                     /translation="MSKGTTGAAAGGGALYGLGIFGALLYYWQQADSFWEYVWAIFPK
                     AIFWPAYMVYEGFRALGA"
     gene            complement(562..1170)
                     /locus_tag="VF195_09820"
     CDS             complement(562..1170)
                     /locus_tag="VF195_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581153.1"
                     /translation="MTGTGTAQELASPLGSVDLGRLVFTRGASHLTIGVDSSMQELYR
                     ARFEGKVPEIRTDGGSVTIAYRMSLRPPRGEIVLSGRIPWDIKASWGISDVVADLEDL
                     DLVGLEISGGSSQVEVRLPRPKQAVRVRIGGGASKVELIRPTGVPVQVHIGSGVSTLV
                     VDDFRLASAGGKTDWRSPDYDQTAARYDIQIGAGASKVTIRS"
     gene            complement(1749..3062)
                     /locus_tag="VF195_09825"
     CDS             complement(1749..3062)
                     /locus_tag="VF195_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6581154.1"
                     /translation="MKRRAVIVGSGAGGATVAKELQGECDVTVVEAGKDFRRATLDRR
                     SIERLRRSRLLVDPRLVRLVYPPIRFRRTPDMWLVNGIGTGGTTTMATGNGIRMDEDL
                     RALGLDLEREFDEIAREIPITTAHQRRWHPTTRRLLRTFDDLGLNPVVLPKMGDAERC
                     RHCGRCMLGCPYGRKWDSRAYLDKAVRRGARLMTGSTVTEVVIEDGRATGVVTRTKAL
                     HGFVPADLVVLAAGGFGTPAILERSGIGCEPTLFVDPVLTVAARIPNAWQCNEIEMPF
                     VVQREGFILSPYFDWISSLFHPEWRHRLQDIVGIMIKLADESVGSVSGRKVHKILTPT
                     DRDRLDEGVGIATEILAAFGADPDEVFLGTVHAGHPGGMLPLTEASAPTMHDDRLPEN
                     VFVADATLFPRSLGNPPILTIIAMAKRVARICTAAGAVSRLSQCG"
     gene            complement(3059..3607)
                     /locus_tag="VF195_09830"
     CDS             complement(3059..3607)
                     /locus_tag="VF195_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581155.1"
                     /translation="MSLRLRALELHVPDVVARSALRRLFEATVAAFGCEPEDVDGLDH
                     RALLERYASFTSTCAERALAGHTDLDAVSRRMWAHAYALGAALRRGLGVRTRADALRA
                     ARIAYRMIGIDLRADEHGDVTVDRCAFAAGYSPEVCRLMSALDAGLFAGLSDGGRLAF
                     SERITEGRPRCLARISWQEASP"
     gene            complement(3604..>3820)
                     /locus_tag="VF195_09835"
     CDS             complement(3604..>3820)
                     /locus_tag="VF195_09835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013826673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581156.1"
                     /translation="AEMNRRLSHPEQLKRWAVLPNDLSIEGGELTANLKLRRHEVEAR
                     FHGEIERLYEEHSTAPIRNAVGAGARP"
BASE COUNT          623 a         1333 c         1222 g          642 t
ORIGIN      
        1 cgctccgaga gccctgaacc cttcgtagac catgtacgcg ggccagaaga tcgccttcgg
       61 gaagatcgcc cacacgtact cccagaacga gtcggcttgc tgccagtagt acaacaacgc
      121 gccgaagatc ccaaggccgt aaagagcgcc gccaccagcg gcagcacccg tggtgccctt
      181 cgacatcgct cacccctcca gtcgaccgtc ctggggacct gtaccgaagg ctactctcgc
      241 gtttcctaga gctggtcatc tacgtcgaag ggacgtggcc tggtccggtc tgccggatcg
      301 ctcaacgccc gccagttggc agctctggtg aggcgctggg cgaccgggcg catcatcaga
      361 acgatcggcc aagcgaagag atcacccttc ttgagcgaaa ggccgacagc ctcccaaccc
      421 tgcgtgccca gttcgttcag ccgctccggt ctcaagtccc tctgacttgt tcttgaggcc
      481 acccgcttgt tccaccggga cagccaagta ctccctcttc tcgatcttcc tcatctccca
      541 ttcgtcgctc gcgcagcctc ttcaggatcg gatggtgacc ttgctggctc cggcgccgat
      601 ctggatgtcg taccgggccg ctgtctggtc atagtccgga cttcgccagt ccgtcttccc
      661 gccggcggac gcgagtcgga agtcatcgac gaccagcgtc gagaccccac tcccgatgtg
      721 cacctgaacg ggtacaccgg ttggacggat cagctcgacc ttgctcgccc cgccgcctat
      781 ccggacgcgg actgcctgct tggggcgggg cagcctcacc tcgacctggg agctcccccc
      841 ggagatctcc aggcctacga gatccaggtc ctcgagatca gcgacgacgt ccgagatccc
      901 ccagcttgcc ttgatgtccc acggaatccg gccggacagg acgatctcgc cgcgtggggg
      961 acggaggctc attcggtacg cgatggtcac gctgcctccg tccgtgcgaa tctccggcac
     1021 cttgccctcg aaccttgcgc gatagagctc ctgcatcgaa ctgtccaccc cgatggtgag
     1081 gtgggatgcg cctcgcgtaa acaccaggcg cccgagatcc accgaaccca gcggcgaggc
     1141 cagctcctgc gcagttccgg ttccagtcat cccgctctca tctcctctca cgcgatgatc
     1201 cgatttcacg ttacggagac cggcggagtg accccagggc cgatggtcct cctaagggca
     1261 gtccgaccgg ggcatcccct gaggacgtcc ccatcatggc gggacgttgg tcctccatat
     1321 gcgcttgctg ggagggcgac agtgaaatgt gggcgtcaga ttcgttcttg gaggaaaacc
     1381 gatggaatcg agagatcagc gaatagccgg cctgtcgatc gtgcggttta cgggcgaacc
     1441 atggtcgttg cggtgctccc cttcggggtc accacccgat ggagtagccg atcgggagca
     1501 tcgtgacgtc aatcgccgga gcgctgatgg cagccgtttg gtcggtacgt cgccccacgc
     1561 ggcatcctga gccgggagcc accttcagga ccccagatac cgcatatccg gacgtggaat
     1621 cgggaagtcc tgtcgccgag gagccgaacc ggtcctccac actccgccga tccgccagga
     1681 tgacgaccca ggcggatctt cgtagtcctt gaaagcaggt ctgacgaggc tgggtaatcc
     1741 ccgaacgtct agccgcattg cgagagacgc gagacggctc ccgcggccgt gcagatcctc
     1801 gccacgcgct tcgccatcgc gatgatcgtc aggatcggtg ggttaccgag cgaacgcggg
     1861 aagagcgtgg cgtcggcgac gaagacgttc tcgggcagcc gatcgtcgtg catcgtggga
     1921 gcggatgcct cggtgagcgg gagcatgccg ccggggtgac cagcgtgcac cgtgccgagg
     1981 aacacctcgt ccgggtcggc gccgaacgcg gcgaggatct cggtggcgat cccgacacct
     2041 tcatcgaggc ggtcgcgatc cgtaggggtg aggatcttgt gcaccttcct gcccgacacg
     2101 gatcccacgc tctcgtccgc gagcttgatc atgatgccca cgatgtcctg gaggcggtgg
     2161 cgccattcgg gatggaacag gctgctgatc cagtcgaaat acggcgaaag gatgaagccc
     2221 tcacgctgca cgacgaacgg catctcgatc tcgttgcact gccacgcgtt cgggatgcgg
     2281 gccgcgaccg tgaggaccgg gtcgacgaag agcgtcggct cgcacccgat tcccgagcgt
     2341 tcgaggatcg cgggggtccc gaatcctccg gcggccagca ccacgaggtc ggcggggacg
     2401 aagccgtgga gcgcctttgt ccgcgtcacc accccggtcg cccggccgtc ctcgatcacg
     2461 acctcggtca ccgtgctccc ggtcatcagc cgggcgccgc gccgaacggc cttgtcgagg
     2521 tacgcgcggc tgtcccactt gcgtccatac gggcatccga gcatgcaacg gccgcagtgc
     2581 cggcatcgtt ccgcgtcgcc catcttcggc aggacgaccg ggttcaggcc gaggtcgtcg
     2641 aacgtgcgga gcagacgtcg cgtcgtggga tgccaccggc gctggtgcgc ggtcgtgatc
     2701 gggatctcgc gagcgatctc gtcgaactcc cgctcgaggt cgagcccgag cgcgcggaga
     2761 tcctcgtcca tgcggatccc gttcccggtc gccatcgtcg tcgtcccgcc ggtgccgatc
     2821 ccgttcacga gccacatgtc tggggtccgc cgaaatcgga tgggcgggta aacgaggcgc
     2881 acgagccggg ggtccacgag cagccggctc cggcgcagcc gttcgatcga ccgtcgatct
     2941 agggtggccc gccggaagtc cttgccggct tccacgacgg tcacgtcgca ctcgccctgg
     3001 agttccttgg cgacggtggc gcccccggcg ccgctcccga cgatgaccgc ccgcctcttc
     3061 acggcgatgc ctcctgccag gagatcctgg cgaggcaccg cggtcgtccc tcggtgatcc
     3121 tctccgagaa cgcgagccgg cctccgtccg agaggccggc gaacaggccg gcatcgagcg
     3181 cggacatcaa ccggcagacc tccggcgagt accccgctgc gaaggcacat cggtcgaccg
     3241 tcacatcgcc gtgctcgtcc gcccgtaggt cgatgccgat catccggtag gcgatccggg
     3301 ccgcccgtag cgcgtcggcc cgcgttcgga cgccgagccc gcggcggagc gccgcaccaa
     3361 gggcgtatgc gtgcgcccac atgcggcggg agacggcgtc gagatccgtg tggccggcga
     3421 gcgcccgctc cgcgcacgtg ctcgtgaacg acgcgtacct ctcgaggagt gcgcggtgat
     3481 ccaacccgtc tacgtcctcg ggctcgcatc cgaacgcggc gactgtcgcc tcgaagagac
     3541 gtcgcagcgc cgaacgcgcc acgacgtcgg gcacgtggag ctcgagcgcc cgcaggcgga
     3601 ggctcatggc cgcgctcccg cacccaccgc gttccggatc ggagccgtcg aatgctcctc
     3661 gtacagccgc tcgatctcgc cgtggaacct cgcctccacc tcgtggcgcc ggagcttcag
     3721 gttcgcggtg agctccccgc cctcgatcga caggtcgttc ggcagcacag cccaccgctt
     3781 cagctgctcc ggatgggaca accggcggtt catttcggcc
//
LOCUS       DASUYD010000174         4974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_102347, whole genome shotgun sequence.
ACCESSION   DASUYD010000174 DASUYD010000000
VERSION     DASUYD010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102347"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF195_09840"
     CDS             <1..543
                     /locus_tag="VF195_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011597341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HEX6581157.1"
                     /translation="AGVHHDIDEIEVEVFSGDPKVSRRIYELSREVADFLRATRPLID
                     VITALRGGFAKYDVDERLQDHLRDVEDHLIQIIERVDEFRVALRDMLTVNATLVAQRQ
                     NEEMRNLAEAANSQNEEVKKISAWAAILFAPTLIGTVYGMNFVHVPELDWILGYPFAL
                     SLMVVTCVGLYALFKRRGWL"
     gene            643..1116
                     /locus_tag="VF195_09845"
     CDS             643..1116
                     /locus_tag="VF195_09845"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6581158.1"
                     /translation="MSQSGPDRELISEEDRLWTELHDLVGSMPADKVEEPGYFAEGWS
                     AKDLVAHVGCWLAEAGVVLDRLRSGTYRPEEIDIDAMNAQFYGSMRDVAFHDVRAQAF
                     TARSRMLRAWRSLPVGSPEADRWIRKSGPEHYAEHIPRLREWVREIGREDPGPRG"
     gene            1122..2288
                     /locus_tag="VF195_09850"
     CDS             1122..2288
                     /locus_tag="VF195_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581159.1"
                     /translation="MEIPERYVRLCLRVGRHLDGFVDSFIGPSEMEQTVEAEEAVDPK
                     GLRDEAQLLLDGLGDADLSEDRRRWLRGQLESLACVTDRLAGEEITWADEVERCLGVR
                     PSRTDTSVLEEVHGRLDAALQGNGSLRERYNAWDANNAVPREKLVPALERLQQVLGPR
                     AHAFAPMPAEESVSYELVSDVPWIAYNRYEGRHHSRVEVNADLPVSVVLLVSLAAHEA
                     YPGHHTERVAKDVHLYRHLGRMETSVAISAPESLVSEGIATNALDEALGREPFAVVAD
                     VLAGIDLRFDPVEAHEVHEAELALYAPAVNAAFMLHEEGASTDDVEGFLREWALESNE
                     RTARTVAFLTDPSSRAYVSAYPDGRRLCRDFADRAPGNFTRLLTEQLTATDLLP"
     gene            2338..3522
                     /locus_tag="VF195_09855"
     CDS             2338..3522
                     /locus_tag="VF195_09855"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX6581160.1"
                     /translation="MTDFPSTVEHMYGSFASTDLDDGGLIGVADALNRHIASAQVALL
                     GVIAEVDRRRAWQDSGARDVAHWLSIRYGMSWWKADRWIKAAAALHDLPGITGALENG
                     VLGIDKVVELCRFANAENEGELIGWASDATCATIRRRADLEVRAAREETREFERNRSL
                     RYWYEDGGRRFGLEARMPSAQGAIVAKALERVIDQVPHMPAEVEGILIEARRADALVA
                     LCSQRIAADADADRATVVVHASLDALAGRRNAETDHGAVLPPEALQRLACDARVQVVA
                     ENDAGDAISLGRTRREPSTSMIRQLRHRDRGCRFPGCGSTVFANAHHIVWWSRGGATD
                     LSNLLLICGFHHRLLHEHGWTVERADDGRVRWFRADGSRYRGGPERATTHPHDDEFLD
                     TG"
     gene            3597..3809
                     /locus_tag="VF195_09860"
     CDS             3597..3809
                     /locus_tag="VF195_09860"
                     /inference="COORDINATES: protein motif:HMM:NF021471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEX6581161.1"
                     /translation="MARTRFEVVPEGDRWVVREGKRSTPPLDTMDLAVSAATRRAQES
                     APSEVVIVHEDGTLEEARTFGDADAS"
     gene            3874..4548
                     /locus_tag="VF195_09865"
     CDS             3874..4548
                     /locus_tag="VF195_09865"
                     /inference="COORDINATES: protein motif:HMM:NF014997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEX6581162.1"
                     /translation="MSTPTDPAGLPVIGGEQSPRARGWIHRVSFLVAIPAGLVLLVLA
                     PSAAARVAVAIYAASLVTVFWVSSTYHLGVWSAEESTRWQRRDHAAIFLLIAGSYTPY
                     CVLALEGPWRVWMLAAVWVGAGLGILVKVYRVDLHVLSGFMYLGLGWAAVIAFPRFVR
                     ALGPAPVILTVVGGLLYSLGALVLAMHRPKLFPRTFGYHEVWHAATVAAAACHYTAVL
                     LMVIEA"
     gene            complement(4603..>4974)
                     /locus_tag="VF195_09870"
     CDS             complement(4603..>4974)
                     /locus_tag="VF195_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581163.1"
                     /translation="NGGGGGNGGGGGNAGGGGNAGGGGNGGGGGNAGGGGNAGGGGNA
                     GGGGNGGGGGNGGGGIEDSGGGDEGAGEPGEVEPGNGGPGEGGGPPEDPGNGNGGGNG
                     GGPPEDPGNGNGGGNGEGKDR"
BASE COUNT          760 a         1694 c         1683 g          837 t
ORIGIN      
        1 gccggtgtcc atcacgacat cgacgagatc gaggtcgagg tgttcagcgg cgatccgaag
       61 gtatcccgcc gcatctacga gctgtcacgg gaagtggcgg actttttgcg cgccactcgc
      121 cccttgatcg acgtcatcac ggcgctccgt ggaggattcg ccaagtacga cgtcgatgag
      181 cgactccagg accatctccg cgatgtcgag gaccacctga tccagatcat cgagcgcgtc
      241 gacgagttcc gggtggccct ccgcgacatg ctcacggtga acgccacgct cgtcgctcaa
      301 cgacagaacg aggagatgcg caatctagcg gaggctgcga attcccagaa cgaggaggtc
      361 aagaagatct ccgcctgggc cgcgatcttg ttcgcgccaa ccctcatcgg gaccgtctac
      421 gggatgaact tcgttcacgt gcccgagctc gactggatcc tcggctaccc gttcgcgctc
      481 tcgctcatgg tcgtgacgtg tgtcggcctc tacgccttgt tcaagcggag gggttggctc
      541 taggcgtgtg tcatcccact caccgaggcg ctcgtcgagc gtagagctcc gcgacgaggg
      601 gctccgtcgt cggaggggtc gcgcgtcgtc atactgaccc gcgtgtcgca gtcgggtccg
      661 gaccgggagc tgatctccga agaggatcgg ttgtggacgg agctgcacga cctcgtcggc
      721 tcgatgcccg cggacaaggt cgaggaaccc ggctacttcg ccgagggatg gtccgccaag
      781 gatctcgtcg cacacgtcgg gtgctggctc gccgaggccg gggtcgtcct ggaccggctc
      841 cggtccggca cgtatcgacc cgaggagatc gacatcgacg cgatgaacgc gcagttctac
      901 ggctcgatgc gcgacgtcgc gttccacgat gtgcgggcgc aggcattcac ggcccggagc
      961 cggatgctgc gggcgtggcg ctcactcccg gtgggatcgc ccgaagccga tcgctggatc
     1021 cggaagtcgg gccccgagca ttacgccgag cacatcccac gactccggga gtgggtgagg
     1081 gagatcggcc gggaggaccc cggtcctcga gggtgacggc cgtggagatc ccggagcgat
     1141 acgtgcggct ctgcctgcgc gtgggccggc atctcgacgg gttcgtcgac tcattcatcg
     1201 gcccctcgga gatggagcag actgtcgagg ccgaggaggc cgtcgatccg aagggcctcc
     1261 gcgacgaggc gcagctcctc ctcgacggcc tcggcgacgc cgacctctcg gaggaccgcc
     1321 ggcgatggtt gcgtggccag ctcgaatccc tcgcatgcgt caccgaccgg ctcgccggtg
     1381 aggagatcac ctgggcagac gaggtcgaac gatgcctcgg tgtccgccct tcacggaccg
     1441 acacgagcgt gctggaagag gtccacgggc gactggacgc agcccttcag gggaacggct
     1501 cgctccgcga gcgatacaac gcctgggatg cgaacaacgc ggttccacgc gagaagctcg
     1561 tccccgcgct cgagcgcttg cagcaggtcc tgggtccccg ggcgcacgcg ttcgcgccga
     1621 tgcctgccga ggagtccgtg agctacgagc tcgtctcgga cgtaccgtgg atcgcctaca
     1681 accggtacga gggcagacac cacagtcgag tcgaggtcaa tgccgatctc ccggtctccg
     1741 tcgtgctgct cgtgtccctc gccgcccacg aggcgtatcc aggtcatcac accgagcggg
     1801 tggcgaagga tgtccacctc taccgacacc tcggccgtat ggagacgagc gtcgcgatct
     1861 ccgcccccga gtcactcgtc tccgaaggga tcgcgaccaa tgccctggat gaggcgctcg
     1921 gtcgtgagcc cttcgcggtc gtcgccgacg tcctggcagg catcgacctc cggttcgacc
     1981 ctgtcgaggc gcacgaggtc cacgaagcag agctcgccct gtacgcgccg gcggtgaacg
     2041 ccgccttcat gctccacgag gagggcgcgt ccacggacga tgtggaggga ttcctgcgcg
     2101 agtgggcgct cgaatcgaac gagcgaacgg cgcggaccgt cgcgttcctc accgatcctt
     2161 cgtcccgcgc gtacgtgtcg gcgtacccgg acgggcggcg actctgccgg gacttcgccg
     2221 atcgggcgcc cggcaacttc acaaggctcc tgaccgagca gctcaccgcc accgaccttc
     2281 ttccctgagc cagccttccg agacgatctc gccagatgtg cggtagatca ctgcgcggtg
     2341 acggacttcc ccagtacggt cgaacatatg tacgggtcct tcgcgagcac cgatctggac
     2401 gacggcggat tgatcggcgt cgccgatgcg ctgaaccgcc acatcgcctc ggcgcaggtg
     2461 gcgctgctcg gcgtcatcgc cgaggtcgat cgacggcgag catggcagga ctcgggcgct
     2521 cgagacgtgg cgcattggct ctcgatccgt tacggcatgt cctggtggaa ggcggaccgg
     2581 tggatcaagg cggctgcggc gctccacgat ctcccgggca tcaccggcgc gctcgagaac
     2641 ggcgtgctcg ggatcgacaa ggtggtcgag ctgtgccggt tcgccaacgc cgagaacgag
     2701 ggtgagctca tcggctgggc gagtgatgcc acgtgcgcca cgatccgtcg tcgcgcggac
     2761 ctcgaggtgc gggctgcgcg ggaagagacc agggagttcg agcggaaccg atccctgcgg
     2821 tattggtacg aggatggcgg acgtcggttc ggcctcgaag cgcgcatgcc gtccgcgcag
     2881 ggtgccatcg tcgcgaaggc gctcgagagg gtcatcgatc aggtcccgca catgccggcc
     2941 gaggtcgaag ggatcctcat cgaagcgcgt cgcgccgacg ccctcgtggc gttgtgctcc
     3001 cagcgcatcg cggccgacgc cgacgcggac cgggcgaccg tggtggtcca tgcgtcgctc
     3061 gatgccctgg cgggacgacg caacgcggag accgatcacg gagcggtctt gccgcccgag
     3121 gcgctccagc gtctcgcgtg cgacgctcgg gtccaggtcg ttgccgagaa cgatgccggc
     3181 gatgcgatct cgttgggtcg cacgagacga gagccctcca cctcgatgat ccggcagctt
     3241 cgccatcgcg accgaggatg ccggttcccc ggatgcggct cgaccgtgtt cgccaacgcg
     3301 caccacatcg tctggtggtc acgaggaggc gccacggacc tcagcaacct gctcctcatc
     3361 tgtggcttcc atcaccggct cctccacgag cacggatgga cggtcgaacg ggccgacgat
     3421 gggcgagttc gatggttccg ggctgacggc tctcgctacc ggggtggtcc cgagcgcgcg
     3481 acgacgcacc cgcatgacga cgagttcctc gacaccggtt gaggccctct gcttgcctcg
     3541 attaccccgg ggcatcgagg ggtaaggcgg aaccgacgcc gaaggaggga gtcgtcatgg
     3601 cgagaacccg gttcgaagtc gttcccgagg gcgatcggtg ggtcgtgcga gaaggcaaga
     3661 ggtcgacgcc gccgctggac acgatggacc tagccgtgag cgccgcgacc cgacgcgcac
     3721 aggagtcggc accgtccgaa gtcgtcatcg tccacgagga tggcaccctc gaggaagccc
     3781 ggacgttcgg cgacgccgac gcttcctgac gcggctctgg acatcgtctc gtccggatgg
     3841 gcggaagctt ctccatgcgg tagaagttct gaggtgagca cccccacgga tccggcgggt
     3901 ctgccggtca tcggagggga gcagtcgccg cgggcacgtg gctggatcca tcgggtgtcg
     3961 ttcctggtgg cgatcccggc cggtctcgtc ctgctggtgt tggcaccgtc ggcagccgct
     4021 cgcgtcgcag tcgccatcta cgctgcgagt ctcgtcacgg tcttctgggt cagctcgacg
     4081 taccacctcg gcgtgtggtc cgcggaggag tccacccgat ggcagcggcg ggaccacgcg
     4141 gccatcttct tgctgatcgc cgggtcctac acgccgtact gcgtgctcgc gctggaggga
     4201 ccctggaggg tctggatgct cgctgcggtc tgggtcggtg ccgggctcgg gatcctggtg
     4261 aaggtgtatc gagtcgacct gcacgtcctc tctgggttca tgtacctggg tctcgggtgg
     4321 gcggcggtga tcgcgttccc gcgattcgtc cgggcgctgg gacccgctcc ggtcatcctg
     4381 accgtggtgg gcggactcct ctactcactc ggagcgctcg tcctggcgat gcacagaccg
     4441 aagttgttcc cacgcacgtt cggataccac gaggtctggc acgcagcgac cgttgccgcc
     4501 gcggcgtgtc actacaccgc agtactcctg atggtgatcg aggcgtgaag ccggatcgcg
     4561 cggtacagcc gacgggatca tgaacgtcgc aactcgccgc ggctagcggt ccttcccttc
     4621 gccgttcccg ccaccgttgc cgttgcccgg gtcctcgggt gggccgccac cgttcccgcc
     4681 accgttgccg ttgcccgggt cctcgggtgg gccgccaccc tcacctggac cgccgttccc
     4741 tggttcgacc tcacccggct ccccggctcc ttcgtccccg cctcctgagt cctcgatgcc
     4801 accgccgccg ttgccaccac cgccgccgtt gccaccaccg ccggcgttgc caccgccacc
     4861 ggcgttgcca ccgccgccgg cgttgccacc gccgccgccg ttgccaccac caccggcgtt
     4921 gccaccgcca ccggcgttgc caccaccgcc gccgttgcca ccaccgccgc cgtt
//
LOCUS       DASUYD010000175         3487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1868574, whole genome shotgun sequence.
ACCESSION   DASUYD010000175 DASUYD010000000
VERSION     DASUYD010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3487
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1868574"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF195_09875"
     CDS             <1..437
                     /locus_tag="VF195_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012399203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEX6581164.1"
                     /translation="MHVGIRGPLYSSRDMVDDASFGFRTIRTEDVHREGVDGVVQRIR
                     ERVGDHPLYISIDIDSLDPAHAPGTGTPEIGGLTSRELLMILRGLVGLRLVSADVVEV
                     SPPYDHADLTSLAAATIVYELVCLLASGPPFASAETNMTRDG"
     gene            437..1315
                     /locus_tag="VF195_09880"
     CDS             437..1315
                     /locus_tag="VF195_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HEX6581165.1"
                     /translation="MFLWPEGKRAALAVTFDMDAEAAVLAVDEKYARRPSIMTHQQYG
                     PVTAVPRLLKLLDALEIRVSFFIPGFSAERHPWTVKAIVSAGHEICHHGYLHRPPGLI
                     DAVTERTELERGLEALDRTAGTRPTVFRAPWWETSDVTLDLLAEYGFTCDSSLFDRDE
                     PYRLAVGSSELVEVPISWALDDWEKYAFLPDPPTGSGVIEPPARVLETWWEEVEAYRE
                     VGGCCVLTMHPFLSGRPARAIALRRLLERALALPDLWIATIGEIAAHTAASDVPARTL
                     SLPTIGEGPAEPVPGS"
     gene            1327..2769
                     /locus_tag="VF195_09885"
     CDS             1327..2769
                     /locus_tag="VF195_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HEX6581166.1"
                     /translation="MDTNREEHLAYEGESGNPVYGLERRGVDYVPESERHMRLRNLAV
                     FWVGTNLYPFNIVLGVLAYTLGLSVWLTLLTLAAGVLLSYWLVGLTSIPPARAGIPTM
                     AVSRAPFGLRFARINAALAWLVGIFFEIINASIGVFAVLALFAFLGWDDSGTSGKLIG
                     LAFVYALSVLLAFLGHGTVVHVQRFFSIALGVISIVVLIEALPDLDVGASLGGDYDLW
                     GTLSAMAIILGIVIAGHVAYVIAAADYPRYLPSDTPAKDIVRTVLWSAGGSGLLLGIV
                     GILLASQTDADLVVDTFGGVESLMPSWLYLPFLLAAIGGSLTNLMLTMYSAGLAAQAA
                     GLPIKRYHAVMVDAVLATIGLVYVLFFDEALLTTINDIVVNTIIWIGPWGVIWIYDAW
                     RRNWNIDPVAAHGGPESPYWGSGGVYWPGAIALIAGMVAAWTSISVPRYAGPISENLL
                     NFTDLSWLVGPLVALAVFIPLDRAQRPHVG"
     gene            2771..3457
                     /locus_tag="VF195_09890"
     CDS             2771..3457
                     /locus_tag="VF195_09890"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6581167.1"
                     /translation="MAGVAVITGAASGLGAATARRLRQEGLAVASVDLYAAADANLSF
                     ELDVTDADAIEDAVERTEEQLGPVEYLMSAAGVYELARLGEIGPEGWKRMFSIHIDGT
                     ANACRSVYRRMMERGAGSIVTIGSELGLMGDPEAAHYAAAKGAIHAFTKSLAMEAVRH
                     GVRVNCVAPGPADTPLLKQDMRSPQYVNSLVLQRIVRPEEVADTVAFLMLERHNFVGA
                     VLSPNAGAVV"
BASE COUNT          485 a         1174 c         1220 g          608 t
ORIGIN      
        1 cgatgcacgt gggcatccgt ggtcctctct acagctcgcg ggacatggtc gacgacgcga
       61 gcttcggctt ccggacgatc cggaccgagg acgtccaccg cgaaggggtc gacggggtcg
      121 tccagaggat ccgcgagcgg gtcggggatc atccgctcta catctcgatc gacatcgact
      181 cgctcgatcc ggcccatgcc cccgggacgg ggacgcccga gatcggtggg cttacgtcgc
      241 gcgagctcct gatgatcctc cgcggtctgg tgggtctccg tctcgtgtcg gccgacgtcg
      301 tggaggtctc cccgccctac gatcacgccg acctcacctc gctcgctgcc gcgaccatcg
      361 tgtacgagct cgtgtgcctg ctcgcgagcg gcccgccgtt cgcgagcgcc gagacgaaca
      421 tgacgcggga cggctgatgt tcctctggcc cgaggggaag cgcgcggcgc tcgcggtgac
      481 gttcgacatg gacgccgaag cggccgtcct cgccgtcgac gagaagtacg cccgacgtcc
      541 ctcgatcatg acgcaccagc agtacggacc cgtgacggcg gtcccccgtc tcctgaagct
      601 gttggacgcg ctcgagatcc gggtcagctt cttcatcccg ggcttctcgg cggagcgcca
      661 tccgtggacc gtgaaggcca tcgtgtcggc gggacacgag atctgccacc acggctacct
      721 ccatcggccg cccgggttga tcgacgccgt gacggaacgg acggagctgg agcgtgggct
      781 cgaggcgttg gatcgcaccg cgggcacccg tcccacggtc ttccgggcgc cctggtggga
      841 gacgtcggac gtgaccctcg atctcctggc cgagtacggc ttcacctgtg actcgagcct
      901 cttcgatcgg gacgagccgt atcggctcgc cgtcggctcg tccgagctcg tggaggtacc
      961 gatctcatgg gccctcgacg actgggagaa gtacgccttc ctgccggacc ctcccaccgg
     1021 ctccggtgtg atcgagccgc ccgcccgggt cctcgaaacc tggtgggagg aggtcgaggc
     1081 ctatagggag gtcggcggct gctgcgtgct gacgatgcat ccgttcctgt ccgggcgacc
     1141 ggcgcgggcg atcgcgctgc gccgtctcct cgagcgtgcg ctcgcgctcc cggacctgtg
     1201 gatcgccacg atcggcgaga tcgccgcgca caccgcagcc tcggacgtgc cggcacgaac
     1261 cctgtcgttg cccaccatcg gagaggggcc ggccgagccg gtccccggtt cgtagagggg
     1321 agacacatgg acacgaaccg cgaggagcac ctggcgtacg agggcgagag cggcaacccc
     1381 gtctacggcc tggaacgtcg cggggtcgac tacgtccccg agagcgagcg gcacatgcgt
     1441 ctcaggaacc tcgccgtctt ctgggtcggg acgaacctct acccgttcaa catcgtcctg
     1501 ggcgtgctgg cgtacacgct cggcctgtcg gtctggctca ccctgttgac cctcgcggcg
     1561 ggcgtgctgt tgagctactg gctcgtgggg ctcacgtcga tccccccggc gagggccggg
     1621 atccccacga tggcggtgag tcgcgctccg ttcggcctgc ggttcgcccg gatcaacgcc
     1681 gcgctcgcct ggctcgtcgg catcttcttc gagatcatca acgcctcgat cggcgtgttc
     1741 gcggtgctcg cgctgttcgc tttcctcggc tgggacgact ccggaaccag cgggaaactg
     1801 atcggactcg cgttcgtcta cgccctctcc gtgctcctcg cgttcctcgg ccacggaacc
     1861 gtggtccacg tgcagcggtt cttctcgatc gcgctcggcg tgatctcgat cgtggtcctg
     1921 atcgaggcgc tgcccgatct ggacgtcggc gcgagcctgg gcggcgacta tgacctgtgg
     1981 ggcaccctct cggcgatggc gatcatcctg gggatcgtga tcgcgggcca cgtcgcgtac
     2041 gtgatcgcgg ccgccgacta ccctcggtac cttcccagcg acacccccgc gaaggacatc
     2101 gttcgcacgg tgctgtggtc cgccggtggg tcggggctgc tgctcggcat cgtcgggatc
     2161 ctgctggcgt cgcagacgga cgcggacctc gtggtggaca cgttcggtgg cgtcgagtcc
     2221 ctgatgccgt cgtggctcta cctgccgttc ctgctcgccg cgatcggtgg gtcgctgacg
     2281 aacctgatgc tcacgatgta ctccgcgggc ctcgctgccc aggctgcggg gctgccgatc
     2341 aagcgctacc acgcggtgat ggtcgacgcg gtcctggcca cgatcggtct cgtctacgtg
     2401 ctcttcttcg acgaggccct cttgaccacg atcaacgaca tcgtggtcaa cacgatcatc
     2461 tggatcggtc cgtggggcgt gatctggatc tacgacgcgt ggcgccggaa ctggaacatc
     2521 gatccggtgg cggcgcacgg cggtccggag agcccctact ggggctccgg cggggtgtat
     2581 tggccggggg cgatcgcgct gatcgccggg atggtggccg catggacctc gatcagcgtg
     2641 ccacggtacg cgggcccgat ctccgagaac ctcctgaact tcacggacct gtcctggctg
     2701 gtggggccgc tcgtggcact ggcggtgttc atcccgctcg accgggcaca gcggcctcac
     2761 gttggctgac gtggccggcg ttgccgtcat caccggcgcg gcctccggcc tgggggcggc
     2821 gaccgcgagg aggctccggc aggagggcct cgcggtcgcg agcgtggatc tctatgcggc
     2881 ggccgacgcc aacctctcgt tcgagctcga cgtgaccgac gcggacgcga tcgaggacgc
     2941 cgtcgagcga acggaggagc agctcggtcc ggtcgagtac ctgatgagcg cggccggggt
     3001 gtacgagttg gctcgcctcg gcgagatcgg ccccgaaggg tggaagcgga tgttctcgat
     3061 ccacatcgac gggaccgcga acgcgtgccg cagcgtctac cggcggatga tggagcgcgg
     3121 cgcgggttcg atcgtcacga tcgggagcga gctcggattg atgggcgatc cggaggcggc
     3181 gcattacgcg gcggccaagg gcgcgatcca cgcgttcacg aagagcctgg cgatggaggc
     3241 ggtccgccat ggggtcaggg tcaactgcgt ggcgcccggg cctgccgaca caccgttgct
     3301 caagcaggac atgcgctcgc cccagtacgt gaactcgctc gtcctccagc gcatcgtgcg
     3361 gccggaggag gtcgccgaca cggtggcctt cctgatgctc gagcgacaca acttcgtggg
     3421 ggcggtcctg tctcccaacg ccggggcggt cgtctgacat gcgctacgag ggcatcgagg
     3481 gacgcgt
//
LOCUS       DASUYD010000176         6407 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_545643, whole genome shotgun sequence.
ACCESSION   DASUYD010000176 DASUYD010000000
VERSION     DASUYD010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6407
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545643"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF195_09895"
     CDS             <1..183
                     /locus_tag="VF195_09895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Phosphotransferase system, enzyme I; transfers the
                     phosphoryl group from phosphoenolpyruvate (PEP) to the
                     phosphoryl carrier protein; part of the
                     phosphoenolpyruvate-dependent sugar phosphotransferase
                     system (PTS), a major carbohydrate active-transport
                     system; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX6581168.1"
                     /translation="TAGGSVGAHLFEVARSLGVPAVTGVELSGAAEGSLAAIDGDAGT
                     VFVLPLARARTAEAGA"
     gene            224..1249
                     /locus_tag="VF195_09900"
     CDS             224..1249
                     /locus_tag="VF195_09900"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX6581169.1"
                     /translation="MSAQTERANRTSTIDIHCHVMSLESEELVRPHFTPDKDPFFRYS
                     GPSSDAYNQRHFSEIVPKLSLPEERLRDMDRMGVDIQAISVAPPQYYYWAEPDLGARA
                     SRIQNDNLARIVREHPDRFVGLGTLPLQDVDASLVELDRLVHELGFNGVEICTSVNGA
                     DFDDRRFEPLLARFEELDLLVVMHPNGFSHGERLAAYYLINTVGMPLDSTVSISRMIF
                     GGVLERHPDLKICVVHGGGYVPFYPARFDHAYHAREDCREFISRPPSTYLRQLYFDTM
                     VFDPEDLAMLIERYGAEHVLLGSDYPYDMGEEDPVGLVKSVDGLGNKELALVLGGNAA
                     RLLKLDV"
     gene            1259..2239
                     /locus_tag="VF195_09905"
     CDS             1259..2239
                     /locus_tag="VF195_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009720124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HEX6581170.1"
                     /translation="MGIVVVTDHVFANLDQEREILAAAGHQLRFERDVKVREEVLEAV
                     RSADAVLNCYMPIPAEVISSMTRCRIIARYGIGLDTIDIPAASEQGILVSNVPDYCID
                     EVSDHALGLILALGRGIARLDRSVRSGTWDVMIARPLHRLRGKTLGLVGFGRIARRLA
                     EKSLPIGFRVLATDPFVDAEAIRGAGVEPVDLPTLLAGSDVVSMHAPLGEDTRHLIGV
                     PELAMMRPGAFLVNTSRGPLVDPDAVLQALEAGALAGVALDVLEQEPPDLSHPLLHRE
                     DVVVTPHAAFYSEEAMVEQQRKAAEQVVDALAGRTPPYAVNARGIAERGS"
     gene            complement(2252..3037)
                     /locus_tag="VF195_09910"
     CDS             complement(2252..3037)
                     /locus_tag="VF195_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6581171.1"
                     /translation="MPPVVNLEGVVVRVDGRRVLGPIDLRVRAGERWVLLGPNGSGKT
                     TLLTVAGARRQPSEGNVQVLGVTFGRGDLRSLHPRIGHCGHTLTEMMPPNLTVLDVVL
                     TGRRSTLVTWFDPIDAEDRERARSRLAEAGVVELSGQAFARCSQGERQRVLLARALYG
                     GRELLILDEPSAGLDLPARERLIAALEAAASASGSPVTLLATHHLEEVPPSTTHAALL
                     RSGRLLASGPVDRILTPERLSECFDVEVEVGRRNGRWWAASPT"
     gene            complement(3050..3748)
                     /locus_tag="VF195_09915"
     CDS             complement(3050..3748)
                     /locus_tag="VF195_09915"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6581172.1"
                     /translation="MARKQELLDRDTTAKVVAERLRDEIQHGTLQPGTRLRQNEVARR
                     FGVSTTPVREAFAQLQAEGLVRIDPHRGAVVFHPTAEDLIEFYEIREVLESFAVVRAL
                     PGLRPDVVKDLEALIDRMRRTDDARRWLKLNDEFHLKLYSCAEMPRLASLIENLRDAS
                     TPYIHMFVAKRPPSERANEEHQRILDACVRGDPAEAELAVREHLRSASSDLVRGLGGT
                     SASELTDDALPSAG"
     gene            complement(3823..4860)
                     /locus_tag="VF195_09920"
     CDS             complement(3823..4860)
                     /locus_tag="VF195_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX6581173.1"
                     /translation="MRAMAVIAYGDPLSLIDLPEPEVPAGYALLEVLTCGVCFSDVKI
                     SRGQMPFSEELPLPHVPGHEIFGRVLRTNPPGLLEEGTTGTVFHYWPCGRCASCRRGD
                     ETLCSQLVGWAGFTHAGGFRERITVPVDRLIRVPSSIDPVLAAPMSCALGTAYRSVVV
                     RGGVRAGSSVAILGLGGVGIHAAQVARAAGARVVGFDVHEPTLAAARDLGLDARRSDD
                     GAIGAALGEIAPEGMDVVLDTVGHDETLAAADRLVRTGGRIVGVGYTPSTALSVRTPR
                     LVLGELELVGSRYAHRDDLEHAIGLVASGQVRPIVTLVRPLEEVNEVFAALGSGDVVG
                     RAVLDVAGSSG"
     gene            5142..6194
                     /locus_tag="VF195_09925"
     CDS             5142..6194
                     /locus_tag="VF195_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581174.1"
                     /translation="MRRNTVWFVATMSALILVAAACGGDGDAEEDGGRPVVRFAFSPD
                     PVIDWMNDQGIIPEYEDKWNVRLVTTSTWDEFTFFAGGHGDIVSMATYETPLLEQETG
                     IETVTFGAYNNLRVPVFVLADNPADTLDDLAGATIAVPSTVSSTIVWGMFSKDWYGLD
                     FCIERPDGSDCADYDLIEGDHFENMDLLLRGEIDACVCIPEAAFPYLRTGEVKALYDP
                     THAPWQLYEQEYAAGHKGLNGNNFVARADWFDSHPNEVGFFLDLWERGLQEWAENKEE
                     IIRTYPQHFAVESEEDVDFAVQYMEDYDFFAPTVYLDEEWVANETAVYDLMKSTGWMD
                     ADAEIPRFEPVAPSEP"
BASE COUNT         1009 a         2165 c         2261 g          972 t
ORIGIN      
        1 acggccgggg gcagcgtcgg tgcccacctg ttcgaggttg cgcgatccct cggtgttccc
       61 gctgtgaccg gggttgagct gtccggcgcg gccgagggat cccttgcggc gatcgacggc
      121 gacgccggga ccgtgttcgt cttgcctttg gctcgggcgc ggacggcgga ggcgggggcg
      181 tgagccggtc ggtcgcgcga tcgggacgga acggaggaag acgatgagcg cacagaccga
      241 gcgggcgaac aggacctcga cgatcgacat ccactgtcac gtgatgtcgc tcgagagcga
      301 ggagctcgtg cggccccatt tcacgcctga caaggacccg ttcttccgct actcgggtcc
      361 ctcgagcgac gcctacaacc agcggcattt ctccgagatc gtccccaagc tctccctgcc
      421 cgaggagcgg ttgcgcgaca tggaccggat gggtgtcgac atccaagcga tctcggtcgc
      481 tccaccccag tactactact gggccgaacc cgacctcggc gcccgcgcct cacgcatcca
      541 gaacgacaac ctcgcgcgga tcgtgcggga gcatccggac cggttcgtcg ggctcgggac
      601 cctgccgctc caggacgtcg acgcctcgct cgtcgagctg gaccgtttgg tccacgagct
      661 cggtttcaac ggcgtcgaga tctgcacgag cgtgaacggc gccgacttcg acgaccggcg
      721 gttcgagccg ctgctcgccc ggttcgagga gctggacctg ctcgtcgtga tgcaccccaa
      781 cggcttctcg cacggggagc ggctggccgc ctactacctg atcaacacgg tcgggatgcc
      841 gctggactcc acggtttcga tctcgcggat gatcttcggc ggcgtactcg agcgccaccc
      901 cgatctcaag atctgcgtgg tgcacggcgg cgggtacgta ccgttctacc cggcgcggtt
      961 cgatcacgcg taccacgccc gcgaggactg ccgcgagttc atctcccgcc caccgagcac
     1021 gtacctgcgc cagctctact tcgacacgat ggtcttcgac cccgaggatc tggccatgct
     1081 gatcgaacgg tacggggcag agcacgtgct gctcgggagc gactacccct acgacatggg
     1141 cgaggaggat ccggtcgggc tcgtcaagag cgtcgacggg ctcgggaaca aggaactggc
     1201 gctcgtattg ggcgggaacg ccgctcggct gctgaagctc gacgtctgag gaggcgccat
     1261 ggggatcgtg gtcgtgaccg atcacgtctt cgcgaacctg gaccaggagc gcgagatcct
     1321 ggcggccgcg gggcaccagc ttcgcttcga gcgggacgtc aaggtacgtg aggaggttct
     1381 tgaggccgtc cgctccgcgg acgccgtcct gaactgctat atgccgatcc cggccgaggt
     1441 gatctcgtcg atgaccaggt gtcgcatcat cgctcggtac gggatcgggc tcgacacgat
     1501 cgacatcccg gcggcgagcg agcaggggat cctggtcagc aacgtccccg actactgcat
     1561 cgatgaggtc tcggatcacg cgctggggct gatcctcgcg ctcggacgcg gcatcgcgcg
     1621 gctggatcga tcggtccgct cgggaacgtg ggacgtgatg atcgcccgcc cgttgcatcg
     1681 tttgcgcggc aagacgctcg ggttggtcgg gttcgggcgt atcgcccggc gtctcgccga
     1741 gaagagcctg ccgatcggat tcagggtcct ggccacggat ccgttcgtcg acgccgaggc
     1801 gatccgcggg gccggcgtcg agcccgtcga cctgccgacg ctgctggcgg gatccgacgt
     1861 ggtgtccatg cacgcgccac tcggcgagga cacccgccat ctcatcggcg tgcccgaact
     1921 ggcgatgatg cgtccggggg cgttcctggt gaacacgtct cgcggaccgc tcgtggaccc
     1981 ggacgccgtc ctgcaggcgc tcgaggcggg ggccctggcc ggggtcgcgc tcgacgtgct
     2041 cgagcaggaa ccgcccgacc tctcccaccc gctcctgcac cgcgaggacg tcgtggtgac
     2101 gccccacgcg gcgttctaca gcgaggaggc gatggtcgag cagcagcgca aggccgccga
     2161 gcaggtcgtc gatgccctgg ccgggcggac gccgccgtac gcggtcaacg cgcgggggat
     2221 cgcggagcgg ggctcctagc gcgtctcgcc atcacgtcgg ggacgcggcc caccagcgtc
     2281 cgttgcgtcg gcccacctcc acctcgacgt cgaagcactc gctgaggcgt tcgggcgtga
     2341 ggatccggtc gaccggtccg gaggcgagca ggcgcccgga tcgcagcaag gccgcgtggg
     2401 tggtcgaggg cggaacctcc tcgaggtgat gggtcgcgag gagcgtcacg ggtgatccgc
     2461 tggcggacgc ggccgcctcg agtgcggcga tcaaccgctc gcgggccggg agatccaggc
     2521 ccgccgacgg ctcatccagg atcaggagct ctcgcccgcc gtagagcgct cgcgcgagca
     2581 gcacgcgctg ccgctcgccc tggctgcagc gggcgaaggc ctgacccgag agttcgacga
     2641 ccccggcctc ggccaggcgg gaccgcgctc gctcgcggtc ctcggcgtcg atcgggtcga
     2701 accacgtgac cagggtcgag cggcgtccgg tcagcacgac gtcgagcacc gtgaggttcg
     2761 ggggcatcat ctccgtgagc gtgtggccgc agtgaccgat gcgcgggtgc agcgaccgaa
     2821 ggtcgccgcg cccgaacgtg acgccgagca cctggacgtt cccctcggaa ggctgcctcc
     2881 gcgccccggc gacggtgagc aatgtcgtct tgccgctccc gttcggcccg agcaggaccc
     2941 agcgctcgcc ggcgcggacc cgaaggtcga tcggaccgag cacgcgtcgc ccgtcgacgc
     3001 ggacgacgac gccttcgagg ttcaccaccg gcggcacggg ggaacttcct cagccggccg
     3061 aaggcagggc gtcgtccgtc agttccgagg cggacgtgcc gcccaggccg cggacgaggt
     3121 cgcttgaggc cgaacgcagg tgctcccgga ccgccaactc ggcctcggcg ggatccccgc
     3181 ggacgcacgc atccaggatg cgctggtgct cctcgttggc ccgctcggac ggcggtcgtt
     3241 tcgcgacgaa catgtggatg tacggcgtgg aggcgtcgcg gagattctcg atgagcgagg
     3301 ccagtcgcgg catctccgcg cacgagtaca gcttgaggtg gaactcatcg ttcagcttga
     3361 gccagcgccg ggcgtcatcc gtgcggcgca tccgatcgat cagggcctcc agatccttca
     3421 cgacgtcggg ccggagcccg ggcagcgctc gcacgacggc aaaggattcc aggacctcgc
     3481 ggatctcata gaactcgatc aggtcctccg cggtggggtg gaagaccact gcgccacggt
     3541 gcgggtcgat ccggacgagc ccctcggcct ggagctgcgc gaacgcctcc cgcaccgggg
     3601 tcgtgctcac gccgaaccgg cgggcgacct cgttctggcg caacctggtc cccggttgga
     3661 gggtcccgtg ctggatctcg tcccggaggc gctctgcgac caccttcgcg gtggtgtctc
     3721 ggtcgagcag ctcctgcttc cgggccacgt cggctcgccc tcctcggtcg cggacacgat
     3781 atatgatgcg tggagccttt cacggcagcg caacccttct cctcagcccg aggaccccgc
     3841 gacgtcgagg acggcgcgtc cgacgacgtc gccggacccc agggcggcga acacctcgtt
     3901 cacctcctcc agcggtcgga cgagagtgac gatcggcctg acctgccccg atgcaaccaa
     3961 gccgatcgcg tgctcgaggt cgtcacggtg tgcgtacctg gatccgacca gctccagctc
     4021 ccccaggacg agccgaggcg tccggacgct gagcgcggtg ctcggcgtgt accccacacc
     4081 gacgatgcgg ccgccggtgc ggaccaggcg atctgccgcc gcgagcgtct cgtcgtggcc
     4141 gacggtgtcc aggacgacat ccatcccctc gggcgcgatc tccccgagcg cagcgcctat
     4201 cgcaccgtcg tcgctcctcc gggcgtcgag cccgagatcc cgcgccgccg ccagggtcgg
     4261 ctcgtgcacg tcgaagccca cgacccgggc gcccgctgcg cgggcgacct gtgccgcgtg
     4321 gatcccgacg cccccgagcc cgaggatcgc gaccgaggag cccgcccgaa cgcctcctcg
     4381 cacgaccacg gagcggtacg cggtcccgag cgcgcacgac atcggggcgg ccaggaccgg
     4441 atcgatcgag gacgggaccc tgatgaggcg gtcgacgggc accgtgatcc gctcccggaa
     4501 gccccccgca tgggtgaagc cggcccagcc caccagttgc gagcacaggg tctcgtcgcc
     4561 gcgccggcag ctcgcgcacc gcccgcacgg ccagtagtgg aacacggtcc ccgtcgtccc
     4621 ctcctccagc aggccgggcg ggttggttcg cagcacgcgc ccgaagatct cgtgaccggg
     4681 cacgtggggg agagggagtt cctcgctgaa cggcatctgc ccccgtgaga tcttcacgtc
     4741 gctgaagcag acgccacacg tcaggacctc cagcagcgcg tacccggccg gcacctcggg
     4801 ctcggggagg tcgatgaggc tcaaaggatc gccgtacgcg atgactgcca tggcgcgcat
     4861 cgctcttcct ccggctgagc gtcgggggcc cgagcgggct tgaccgaaca tagtacataa
     4921 tcgacaggcg gcgcgcgcct ggccgttgct tgactgggat tcgatattgt gtacaaactt
     4981 gagacccttg tacgaacgca ggagggcgac gacgaggccc gacggttgca gtcgccggga
     5041 tcggcggccc cgaacccggg aagagcgtgg gcttcgtaga aatcagatgc acggcgcggt
     5101 cgagggggcg cgccggggga tcggagcaga ggaggcggcg gatgcgacgg aacacggtgt
     5161 ggttcgtagc cacgatgagt gcgctgatac tcgtcgcggc ggcatgcgga ggtgacggcg
     5221 acgcggaaga ggatggtggt cgtccggtcg tccgattcgc cttctcgccc gacccggtca
     5281 tcgactggat gaacgaccag gggatcatcc ccgagtacga ggacaagtgg aacgttcggc
     5341 tggtcacgac ctcgacgtgg gacgagttca cgttcttcgc gggcggccac ggtgacatcg
     5401 tgtcgatggc gacctacgag acaccgctgc tcgagcagga gacgggcatc gagacggtga
     5461 cgttcggcgc gtacaacaac ctgcgggtgc cggtcttcgt cctggcggac aacccggccg
     5521 atacgctcga cgacctagcg ggcgcgacga tcgccgtccc cagcacggtg tcatcgacga
     5581 tcgtgtgggg gatgttctcc aaggactggt acgggctcga tttctgcatc gagcggccgg
     5641 atgggagcga ttgcgcggac tacgatctga tcgagggcga ccacttcgag aacatggatc
     5701 tgctcctgcg cggtgagatc gacgcctgcg tgtgcatccc cgaggcggcc ttcccgtacc
     5761 tgcgcacggg cgaggtcaag gccctgtacg acccgacgca cgctccatgg cagctctacg
     5821 agcaggagta cgcggcgggt cacaaggggt tgaatgggaa caacttcgtg gcccgcgccg
     5881 actggttcga cagccacccg aacgaggtgg gcttcttcct cgacctgtgg gagcggggcc
     5941 tccaggagtg ggcggagaac aaggaagaga tcatccggac gtatccgcag cacttcgcgg
     6001 tcgagagcga ggaggacgtg gacttcgcgg tccagtacat ggaggactac gacttcttcg
     6061 caccgaccgt ctacctggac gaggagtggg tggcgaacga gaccgccgtc tacgacctga
     6121 tgaagtcgac cggctggatg gacgcggacg cggagatccc gcgcttcgag cccgtcgcgc
     6181 cgtccgagcc gtaaggaagg agatggccgg cgccgggacg agctgctcgg cgcaacgaga
     6241 caccccggat cggggggcct gactgccccc ggtcgcgtct tgcagatcgg agttcgtctc
     6301 aggtggcgac acggacggca cctccgcagg cggccatgcc tgcccctcgc gtcgagagcc
     6361 gcgcgcaacc gcgcaagccg cgcctgtggg cgtggcggct ggctggg
//
LOCUS       DASUYD010000177         9453 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_989091, whole genome shotgun sequence.
ACCESSION   DASUYD010000177 DASUYD010000000
VERSION     DASUYD010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9453
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989091"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VF195_09930"
     CDS             <1..880
                     /locus_tag="VF195_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581175.1"
                     /translation="AITGQPEDLFARALRRQEFDVTELSLSSYLVVLARATSPYIAVP
                     VFPSRVFRHSAVYVRTDRGIDRPENLAGKTVGVPEFQQTAALWVRGILADRHGVAPSD
                     IRWRSGGLEQPSDGERIAIHVREGIDLRPIEPESTLSGMLAEGRLEGLVSPRPPSCFE
                     QEHPMVRRLWPDHREAERRYYRETGLFPIMHVVAVRRDLSERHPWLAPSVFDAFSEAK
                     RLAIWELEQINFLRVTLPWVDLDGVRDLMGRDFWAYGIEANRAELEAVTRWSFEEGLA
                     GRRLEPSDLFHPSTVG"
     gene            complement(891..1823)
                     /locus_tag="VF195_09935"
     CDS             complement(891..1823)
                     /locus_tag="VF195_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6581176.1"
                     /translation="MARILVADPLLLTEEFGIDAHWAGPEHEVVIPTGFELDELRQHL
                     GQAAGIVTAHHPVTADMIALAPNLRVIAKPGAGVDNIDMAAAVERGVVVTNVPNARGR
                     AVAEHALFMMLYLARHAWMRGDPAWQGTTSVQLGAKTLGIVGFGAIGGHLAGFGTGLG
                     MHVIAHTRTRDRSGSAEPVKFVDRDELLRRADFVVLCVPLTDDTRWMIDRRALASMKE
                     DAYLINVARGPVVATEDLLEEMRSGRLAGAGLDVTDPEPLPADHGLRRLTNVLISPHL
                     AGRTHESQAEALDRMRQNVRAVLAGRSPLDPVEP"
     gene            1918..2226
                     /locus_tag="VF195_09940"
     CDS             1918..2226
                     /locus_tag="VF195_09940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX6581177.1"
                     /translation="MPTDPIVVFASFHPVPGKADELQDLLSWMVENTRTEPGCERYDL
                     YRQDGTAEVFHLFERYRDQGALQAHRAAGYYVDYRRRVADLIEGDVGVIILHPLDVVG
                     "
     gene            complement(2400..3668)
                     /locus_tag="VF195_09945"
     CDS             complement(2400..3668)
                     /locus_tag="VF195_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX6581178.1"
                     /translation="MTATEAARLIAEGSLTSESLVRACLEQIEARESFVQAWTFLDPE
                     LALAQAKVRDAMLAVGPLHGVPVGVKDTFDTYDMPTEYGSPLYAGHRPRRDAATVALA
                     RGDGAVVIGKTVTTEFGFFAPGKTRNPHDRSRTPGGSSSGSAAAVADCMVPVAFGAQT
                     AGSIIRPAAYCGVVGLKPSFGRISRSGVWPFAGSLDTVGTIGRTVADAALLAASASQR
                     ASMRGAESAQPPPRIGMYKTHDWERAAPESRAAVEDAARILDQAGARLTDVPFSDSFG
                     ALVPAHSLVMAYEASHTFAHEVREDRDSLSTELVTLLDQGLATSPDEYDSALRERDAA
                     IPQLARILEDVDVLLTPSVIGEAPLGLGSTGDPVFNRAWTFLGAPAVHLPAFTGPNDM
                     PVGVQLVGPVGGDDRLLTAAIWAEEQLSPR"
     gene            4999..5928
                     /locus_tag="VF195_09950"
     CDS             4999..5928
                     /locus_tag="VF195_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581179.1"
                     /translation="MKRREALASVAVIAVTATLVLAPSAHATYPARNGLIAFMADTGS
                     GYQIYTVRADGRDLRQITHVNGEALNPDWSPDGRKIAFALDECSIAIMRRDGSHIRVI
                     PHAAPHASDVCEADPSFTPDGSRLVFERYDPILDEDAVWSMNLDGGDRVRITDAGAPD
                     PNVSPNGQTVSFLGGTPDGLTALFTAGLDGSQGFQVTTLYGIAFKSDWAPDGQHLVVT
                     DNADDLSRPANIATIAPDGTDLTYLTDYTSPKLRAYVGSYSPDGRWIVFRLEDHGRFS
                     LFRMRPDGGEVRAILGPSKFMPRSIDWGPAASG"
     gene            6014..6940
                     /locus_tag="VF195_09955"
     CDS             6014..6940
                     /locus_tag="VF195_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581180.1"
                     /translation="MRRTLFIATIALSMLMPFANPAAEAKYPGPNGQILFARNEPPDN
                     DLTPFTINPDGTHEFRVLPGIAAECPRWSPDGSKISVPGATVNADGTGFTPMAPSSDP
                     LLNLGCNTWSPDGSRLAVEGFNDDHPEVTGIYTVRASDGGDLMRVTPVGATPTVVIGD
                     YSPDGNRIVFLGEPAHGRRGSLAVFTVNTDGTGLRQITPGVANCCPGPSWSPDGTELL
                     FGSTRGKLLVVHPDGTGLGQIPIRTGGGFSFAFGPGWSPDGKNIVFSLFLETKGQVDI
                     YTARADGTQLVQVTNTPDNEEFADWGAHPLAT"
     gene            7022..7552
                     /locus_tag="VF195_09960"
     CDS             7022..7552
                     /locus_tag="VF195_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigE family RNA polymerase sigma factor"
                     /protein_id="HEX6581181.1"
                     /translation="MRSGAERTEPVAVTSSERFEDFYRREFAAVVGLAYALSGSRWAA
                     EDLAQEAFIAAHRNWERLGTYDQPGAWVRRVVANLSVSAFRRRAVEAKALARIVLGER
                     PAVPELAAEDPEFWHAIRSLPRRQAQVVALYYLEDRPIAEVAEILDVAPGTVKKHLYN
                     GRRSLARRLGIEEGTA"
     gene            7549..8643
                     /locus_tag="VF195_09965"
     CDS             7549..8643
                     /locus_tag="VF195_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581182.1"
                     /translation="MTLDQRARRAAQGFRRGVESMRTTDPASPERFERYRARKQRNQR
                     IGAVLVAGTVAVAALVLLTKTFPRTERNRPAEPSSNGPIVFGRAVPGVDGIRLFTVNA
                     DGTGETALPVTFTDCAAWSPDGTKMQVTGSDFPGAPLRPAIVNADGSGLRVLDVPAPK
                     ELSLGCGAWSPDGTRLALEGFTETGPPELSGLYTVRASDGGDLRKVSDVAGANPEYSP
                     DGTQIVSIGGDPTRDLKPSAGALFVVNTDGSGLRRLTEWGIAQRSGGSWSPDGEWILF
                     QRTGGGLMMVRPDGSDLHEIPIEGLPQGAHATEPRWSPDGTMIVFGVVANGQEDLYIV
                     RSDGTELMQLTDTPDVDERRPAWGPSIVPN"
     gene            complement(9266..>9453)
                     /locus_tag="VF195_09970"
     CDS             complement(9266..>9453)
                     /locus_tag="VF195_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581183.1"
                     /translation="EEKAVWWSPLPEAPPRCERGDEAEISQLLQPVLTSPERSEVQGL
                     DLVRGEDLVLEQLRRIA"
BASE COUNT         1583 a         3206 c         3205 g         1459 t
ORIGIN      
        1 ggcgatcacg gggcagcccg aggatctgtt cgcgcgagcc ctgcggcgac aggagttcga
       61 cgtcacggag ctctcgctca gcagctatct cgtggtgctg gctcgagcca ccagccccta
      121 catcgccgtg ccggtgttcc catctcgggt gttccggcac tcggccgtgt acgtccggac
      181 cgaccgaggg atcgaccgcc cggagaacct ggccggcaag accgtcggcg tgccggaatt
      241 ccagcagacc gcggccctat gggtgcgcgg gatccttgcc gaccggcacg gggtcgctcc
      301 ttccgacatc agatggcgga gcggaggcct ggagcagccg agcgatgggg agcggatcgc
      361 gatccacgtt cgcgaaggga tcgacctccg accgatcgaa cctgaaagca cgctctcggg
      421 gatgctcgcc gaggggcggc tcgaggggct cgtctcaccg cgtccgccat cgtgcttcga
      481 gcaggagcac ccgatggtgc gtcgcctatg gcccgatcac cgcgaggcgg agcgtcgcta
      541 ctaccgcgag acgggcttgt tcccgatcat gcacgtcgtc gcggtgcgac gcgacctgag
      601 cgagcgacac ccgtggctgg caccgagcgt tttcgacgcc ttttccgaag ccaagcgact
      661 tgccatctgg gagttggagc agatcaactt cctgcgggtc acgctgccgt gggtcgacct
      721 cgacggggtc agggatctga tgggacgcga cttctgggcg tacggcatcg aggcgaatcg
      781 agccgagctg gaggccgtaa cccgatggtc gttcgaggag ggccttgccg gaagacggct
      841 ggagccgtcg gatctcttcc acccaagcac ggtcgggtga gccgagcggc ctacggctcc
      901 accggatcga gaggcgaccg gccggccagc accgcccgaa cgttctgacg catccggtcc
      961 aacgcctcgg cctgggactc gtgcgtgcga ccggcgaggt gcggcgagat caagacgttc
     1021 gtgagccgtc ggaggccgtg gtcagcaggg agcggctcgg gatcggtcac gtcgagcccg
     1081 gcccccgcga gccgacccga tcgcatctcc tcgagcagat cctccgtcgc cacgacgggt
     1141 ccgcgggcga cgttgatgag gtaggcgtcc tccttcatcg atgcgagcgc ccgacgatcg
     1201 atcatccagc gggtgtcgtc ggtcaacgga acgcacagca ccacgaagtc ggctcgacgc
     1261 aggagctcgt cgcgatcgac gaacttcacc ggctcggcgg acccgctccg atcacgggta
     1321 cgggtgtggg cgatgacgtg catccccagt cccgtgccga agccggcgag gtgcccaccg
     1381 atggcgccga acccgacgat cccaagcgtc ttcgccccga gttggaccga ggtggtgccc
     1441 tgccaggccg ggtccccgcg catccacgcg tgacgggcga ggtacagcat catgaacaga
     1501 gcgtgctccg ccacggcccg cccacgcgcg ttcgggacgt tggtcaccac gacgccgcgc
     1561 tccacggcgg ccgccatgtc gatgttgtcg acacccgcgc caggcttggc gatgacgcgg
     1621 aggttcggag cgagcgcgat catgtcggcc gtcacgggat ggtgggccgt tacgatcccc
     1681 gccgcttgcc cgaggtgctg ccggagctcg tccaactcga acccggtcgg gatcacaacc
     1741 tcatgttcgg gcccggccca gtgggcatcg atgccgaact cctcggtgag caagagcggg
     1801 tcggcgacca ggatccgcgc cacgctcacc accaccgagg ccgacgtcgg tacactgcgc
     1861 caacactatc ggacgagggt ccccgatctc gggacccggc ccacgcgtga ggtgctcatg
     1921 ccgaccgacc cgatcgtcgt gttcgcgtcc ttccatcccg taccgggaaa ggcggacgag
     1981 ctccaggact tgctgtcgtg gatggtcgag aacacacgaa ccgaacccgg ctgcgagcgc
     2041 tacgacctgt accggcagga tggaacggcc gaggtcttcc acctgttcga gcgctaccgg
     2101 gaccaggggg ccctccaggc gcatcgcgcg gctggctact acgtcgacta ccgacgccgt
     2161 gtcgccgacc tgatcgaagg tgacgtgggg gtgatcatcc tccacccgct cgacgtggtg
     2221 ggctgacgtc tcgggcagcc cggcctgcca ttccccctcc agatgcgaga tgccgggcgt
     2281 agatcggggc gcctctcggg ggtagcggag atcggcctcg cgttgcggtg gcccaaagcc
     2341 acctcgccgc cggcgaggga cgcctgcctc gccgtcgatg acaggacgac cgcgcagcgt
     2401 tagcgagggg aaagttgctc ctcggcccag atggctgccg tgagcagacg gtcgtcgccg
     2461 cccaccggac cgacgagctg cacgccgacc ggcatgtcgt taggcccggt gaacgcaggg
     2521 aggtggacgg ctggcgcgcc caggaacgtc caggctcggt tgaagacggg gtcaccggtc
     2581 gatccgagcc ccagcggcgc ctcgccgatc acgctgggcg tgaggaggac gtcaacgtcc
     2641 tcgaggattc gggcgagttg cgggatggcc gcatcgcgct ctcgcaacgc ggagtcgtac
     2701 tcatccggcg aagtggccag gccctgatcc aagagcgtta ccagttccgt gctgagcgag
     2761 tctcgatcct cccggacctc gtgcgcgaac gtgtgggatg cctcgtaggc catgacaagg
     2821 gagtgggctg ggacgagagc gccgaacgag tcggaaaatg ggacatccgt cagccgtgca
     2881 cctgcctgat cgagtatccg agcggcatct tcgaccgctg cgcgggactc cggagcggct
     2941 cgctcccagt catgggtctt gtacatcccg atcctcgggg gtggttgggc cgactccgca
     3001 ccgcgcatcg atgcgcgctg gctcgccgag gccgccagca gcgcggcatc cgctacggtc
     3061 ctcccgatcg tcccgacggt gtccagggat ccggcaaacg gccatacccc cgagcgggag
     3121 atgcgaccga aggacggctt tagccccaca acgccgcagt acgctgccgg gcggatgatc
     3181 gagcctgctg tttgggctcc gaaggccacg gggaccatgc agtctgcaac ggcggcggct
     3241 gatccgctcg aggatccacc gggcgttcgg gatcggtcgt gcggattgcg ggtcttgccc
     3301 ggagcgaaga acccgaactc ggtcgtcacg gtcttgccga tgacgacggc accatcacct
     3361 cgggcgagcg ccacggtggc tgcgtcgcgc cggggacggt gcccagcgta cagtggtgag
     3421 ccgtactcgg tcggcatgtc gtaggtgtcg aaggtgtcct tcacgcccac cgggactccg
     3481 tgaagcgggc cgacggcgag catcgcatca cggaccttcg cctgggccag ggccagctcg
     3541 gggtccagga acgtccaggc ctggacgaag gactcgcggg cctcgatctg ctcgaggcag
     3601 gcgcggacca ggctctccga agtcagcgac ccttcggcga tcaggcgcgc cgcctctgtg
     3661 gccgtcaact cgtgaagctc tgtcctcacg acaggggctc ccagcaggac agggggcgcc
     3721 gcatggcctt ggcggtcttg gccagcggtc tcggcgcgag cgcgtcatcc ttccccagca
     3781 cacgtgcagg cttcccctcg atgtgaggac ccaagaagtc cgaaccgtgc ccgatcgcgg
     3841 ctcgggctcg ccccgcctcg tggcctcgca gcggcgagcc gccgatctgc gtccctgccc
     3901 ggccgctgga ccttgagacc gcgggggcag gtcctcgttc agaggcccgt ccactgggtc
     3961 ccatgcgtgg aggttgcgac ttgctcactc gatcctcgtt tccctcaacc agtctcacct
     4021 tcccacgtcc cgcgtcgcta gtgggcagtc tgcataatcg gatagttcgc gcatgtcgcg
     4081 ctcggagagc ggcaaaaggc cgtcatcccg cgatcatcgc gtttcggcgc caggatccca
     4141 tctcgtttcc cggcggcgtg tcggtcatga atcggaccca ggaaccggac gtccgaaagc
     4201 gaactccacg aggagaagct ggcgaagctg gatggccttc gccatgggat cgatccggcg
     4261 ctcatcggtg aggccatcga ccgactatcc aagaacgaga gtcgcgtccg tgacgaactc
     4321 agccggatga accgagagcc cgacgagcag agcctcctgc gaggagcgcg tcccagatag
     4381 acagtgcccg gggccggacg ccgacagaat cctcatgtac gggagctccc cttgagctgg
     4441 tggttcaggt gccgtggcgc cggcgccatt agattctgca tgacggccgc ggcccatacc
     4501 ggagcccgcg tccctcgtcg gttcacacga ctgctgaaac gtagaggcat cggcgtaccg
     4561 ctccttgtct gcccgaacgc ggtcttcgga tgggcacagc ggtggctctg gatgtgcaca
     4621 gacgctgatc aactcacagg tcggccgctc cgcggggccg ctttcgctcc gagagatcgc
     4681 tgagtggctc gcaccgaccc gtcgagcgag cgcctcctgg gagatcccag agcggcgcga
     4741 tgccatgacc aggtcgcacg aaccggggct gagagcgttc cgctgaccgc gtcccaccca
     4801 ccggacgaag accggttaac cgggaaacgc acagctctca ttcgagtgat tcccacatcg
     4861 gtgcgctcat cgacgcctct acgggatgga ggaataggta aaccccgggg cggccccagc
     4921 gcgagtgaca aatgacgtct tggggagcgc cgtggaccga tgcacgttcg cgaggcgcgc
     4981 gacggagagg agccgaacat gaagaggcgg gaagcactcg cgagcgtggc ggtcatcgcg
     5041 gtgacggcga cgttggtgct ggcgccgtcg gcgcacgcca cgtaccccgc ccgcaacggt
     5101 ctgatcgcgt tcatggccga tactgggagc gggtaccaga tctacacagt ccgcgccgac
     5161 ggccgcgacc tgcgacagat cacccacgtc aacggcgagg cgctcaaccc ggattggtct
     5221 ccggacggtc ggaagatcgc cttcgcgctc gacgagtgct cgatcgccat catgaggcga
     5281 gatggcagcc acataagggt gatccctcac gccgccccgc acgcctcgga cgtctgcgag
     5341 gcggatccgt cgttcacacc cgatgggtcc aggctggtct tcgagcggta cgacccgatc
     5401 ctcgatgagg acgcggtgtg gagcatgaac ttggacggcg gcgaccgggt gcggatcacg
     5461 gacgcgggtg cccccgatcc caacgtgtcc cccaacggac agacggtcag cttcctgggc
     5521 ggcaccccgg acggtctcac cgccttattc accgcgggtc tcgacggtag tcaaggcttc
     5581 caagtcacga cgctctacgg catcgcgttc aagtccgact gggcccccga cgggcaacac
     5641 ctcgtagtga ccgacaacgc ggacgacctc tcccggccgg cgaatatcgc cacgatcgcg
     5701 ccggacggga ccgacctgac ctacctcacg gactacacct cccccaagct ccgggcctac
     5761 gtcgggtcgt actcgcccga tgggcggtgg atcgtgttcc gcctcgagga tcacgggagg
     5821 ttcagcctgt tcaggatgcg tcccgacggt ggcgaggtgc gcgccatcct cggcccttcg
     5881 aagttcatgc cccgatcgat cgactgggga cctgcggcga gcgggtaaac cggcaacgcc
     5941 caccgttcgt gtaaccctca gcgccgcgct gtcacgacca gatgttccga gacgaacgcg
     6001 aggaggccac acgatgcgcc gaactctgtt catcgcgacg atcgccctct ccatgctcat
     6061 gcccttcgcc aacccggcgg cggaggcgaa gtaccccggc ccaaacggcc agatcctgtt
     6121 cgcgcgaaac gagccacctg acaacgacct gacacctttc acgatcaacc cggacgggac
     6181 acacgagttt cgcgtcctgc ccggcattgc tgctgagtgt ccgcgctggt cgcccgacgg
     6241 aagcaaaatc agcgttcctg gggcgacggt caatgcggac gggaccgggt tcacgccaat
     6301 ggccccctcc tccgacccgc tgctgaacct cggttgcaac acatggtccc ccgacggctc
     6361 ccgactcgct gtcgagggct tcaacgatga tcaccccgag gtgaccggca tctacacggt
     6421 ccgggcctcg gacggagggg acctcatgcg cgtcaccccc gttggcgcga caccgaccgt
     6481 cgtcatcggc gactactcac cggatgggaa ccggatcgtg ttccttgggg agcccgccca
     6541 tggccggcgc ggctccttgg ctgtgttcac ggtgaacacg gacggaaccg gtctcaggca
     6601 gatcacgcct ggcgtggcga attgctgccc gggtccgagc tggtcgccag acggaacgga
     6661 gcttctgttc gggtccacga gggggaagct cctcgtcgtc catccggacg gcaccggtct
     6721 ggggcagatc cccatcagga ccggtggcgg cttctcgttc gccttcggtc cgggctggtc
     6781 gcccgacggg aagaacatcg tgttctccct tttccttgag acgaagggtc aggtggacat
     6841 ctatacggca cgcgcggacg gcacgcagct cgtccaggtc acgaacacgc ctgacaacga
     6901 ggagttcgcg gactgggggg cccatcctct cgcgacataa ggcgctgagg acgaccggac
     6961 gcatcgctca cccgcaacga cctggcgcgt cggaacccat cgcgtcagga tgtcgctcag
     7021 catgcggagc ggtgccgagc ggaccgagcc ggtcgcggtc acctcctcgg agcggttcga
     7081 ggacttctac cggcgcgagt tcgcggcggt cgtgggcctc gcgtacgcgc tctccggcag
     7141 ccggtgggcg gccgaggatc tggcgcagga ggcgttcatc gcggcgcacc gcaactggga
     7201 gcggctcggg acctacgacc aacccggtgc gtgggtgcgc cgggtcgttg ccaacctgtc
     7261 ggtctcggca ttccgacgcc gcgcggtgga ggcgaaggct ctcgcgcgga tcgtcctcgg
     7321 agaacggccc gcggtgccgg agttggccgc cgaggatccg gagttctggc acgccatccg
     7381 ttcgctccct cgccggcagg cccaggtggt cgcgctgtac tacctggagg accggccgat
     7441 cgccgaggtc gccgagatcc tggacgtggc gccgggcacg gttaagaagc atctgtacaa
     7501 cggacggcgc tcgctcgctc gccgtctcgg catcgaggag ggcacggcat gacgctcgat
     7561 cagcgcgccc gtcgcgccgc gcaggggttc cggcgcggcg tcgagtcgat gcggacgacc
     7621 gaccccgcct cgcccgagcg cttcgagcgg taccgtgcgc gcaagcagcg gaaccaacgg
     7681 atcggcgcgg tcctcgtggc cggaacggtc gcggtcgccg cgctcgtcct gttgaccaag
     7741 accttcccgc gcacggagag gaatcgaccg gccgaaccct cgtccaacgg cccgatcgtg
     7801 ttcggccggg cggtcccagg cgtcgacggc atccgcctgt ttacggtaaa cgccgacggc
     7861 acgggcgaga ccgccctccc ggtcaccttc accgactgcg ccgcctggtc cccggacggg
     7921 accaagatgc aggtcaccgg aagcgacttt cccggcgcgc cgctccggcc cgccatcgtc
     7981 aacgccgacg gctccgggtt gagggtgctc gacgtccccg cccctaagga actcagcctc
     8041 ggctgcggcg cctggtcacc cgacgggacg aggctggcgc tcgaagggtt taccgagacg
     8101 ggtcccccgg agctgagcgg tctctacaca gttcgtgcct ccgacggagg ggatctcagg
     8161 aaggtcagcg atgtcgcagg cgcgaacccc gagtactcac cggacggcac gcagatcgtc
     8221 tccatcggtg gcgatcccac ccgcgacctc aagccgagcg ccggcgcgct cttcgtcgtt
     8281 aacaccgacg ggagcgggct ccgacggctc acggagtggg ggatcgcgca gcgatccggc
     8341 ggtagctggt cgccggacgg cgagtggatc ctgttccaac gaaccggagg cggcctcatg
     8401 atggtccggc ccgacggttc ggacctgcac gagatcccta tcgaggggct tccgcaaggc
     8461 gctcacgcga ccgaacctcg ttggtccccc gacggaacaa tgatcgtgtt cggcgtggtc
     8521 gcgaacggcc aggaagacct gtacatcgtg cgaagcgacg ggaccgagct catgcaactc
     8581 acggacacac cggacgtgga cgaacgccgt ccggcttggg gcccgagtat cgttccgaac
     8641 tgacacgcag gcacgggcgc ggcgtcgact ccctcctccc gcactctcga cctatggaag
     8701 cgccaggcct gcttcgtcaa cggggagctc gttcagggcc gggcgcgctg cgctgcgaga
     8761 tgatcgcgag ccttcgtgtg agccatgtgc ttcctctccc gggacggctc tgggtcctat
     8821 tcaccatgcc gcgcggagcg cgctcgagcg ggacccgctc gcggcggacg gagaccgaac
     8881 gcctgcacag gcagcgcgag ggcgaggcgg cgccaacgcg gacggtttgg cgggatgcga
     8941 tcccggccga acgcctgccg gagcatgccg gcgcggcgag caaaggaggt gatcaccggc
     9001 acgaactgaa catcctctga aagcggcagg tcggggcgcg cgccttggcg cgagctcgcg
     9061 cccgtccaag ggaaccgggt ggttcccgcc gccagcggcg cgtgggcggc gcgagagcgc
     9121 agggtgcgag gtcgaaaagc cgcaggtcac ggggctgttt ggccgaaggc cacccaccga
     9181 aggagggggg agggctgtgc ccagaatatg gttcgagaag aaagaaacga cattcgcgcg
     9241 cgcgcgtggt cctcgatgag cgagatcacg cgatcctcct cagctgctcg agcacaaggt
     9301 cctcaccacg gaccaggtca aggccctgta cttcggatct ctcaggagat gtcagcaccg
     9361 gctgaaggag ctgagagatc tcggcctcat cgcctcgttc acaacgagga ggagcttcgg
     9421 ggaggggcga ccaccagact gccttttcct cac
//
LOCUS       DASUYD010000178         4766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1431267, whole genome shotgun sequence.
ACCESSION   DASUYD010000178 DASUYD010000000
VERSION     DASUYD010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4766
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431267"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF195_09975"
     CDS             complement(<1..930)
                     /locus_tag="VF195_09975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6581184.1"
                     /translation="MDPQNPRTIARVFDWQFKGMRWWFRRSVGLAAILGLSLVILAPG
                     AVAQDDDGPDSDDHVILTGGLVVPEGETAKTAVVFNGDVLIDGTVSETLVVFNGRTEI
                     TGTVGEDVVVFNGSVVLRSGARVGGDVVSRQTPQIEDGATVEGSIDDLQKRWDYWDIT
                     FVGRLAWWLAYTVSTLVLGLVLLMLARGLDPASIRALRDRLGATIGFGLLWFVLLPIV
                     AVLLLVTIVGIPLGLFLLFGLGLVYSIGYVVGGLALGRLVVKEPTSRYLAFLAGWGAL
                     RVLALVPFLGGIAWLVGTVLGLGTLWVAARAPSG"
     gene            1417..1653
                     /locus_tag="VF195_09980"
     CDS             1417..1653
                     /locus_tag="VF195_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581185.1"
                     /translation="MKEATRLDPVQADIADWNREIADAAAGKNIAHPPVDVDAFVLET
                     KQVMVEENRAEAKQAALGGTVGRQRGPRSRARPG"
     gene            complement(1718..2245)
                     /locus_tag="VF195_09985"
     CDS             complement(1718..2245)
                     /locus_tag="VF195_09985"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6581186.1"
                     /translation="MNRIRLEGELVILRPLSMDEFDQEWANRLEMDPSVQPIMPTRVA
                     LRVRFERSGIMEGGALDLAIEVAGRMIGGIQTYVPPDRTLPSGAFEIGIVIDEPSMRG
                     KGYGTEAVMLLVGWLFSEAEATQIHMPTVPDNVAMRTVAERLGFETDGTIRYEGQEFD
                     FYVLTRERWVDGGFG"
     gene            complement(2247..3599)
                     /locus_tag="VF195_09990"
     CDS             complement(2247..3599)
                     /locus_tag="VF195_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6581187.1"
                     /translation="MIPQTRYTKASDGTYLAYQAFGEGDQDLLWVPGAATHLEVFWEY
                     PPAARFLRRLATFARVIWFDKRGTGLSDRVSRLPGLETMMDDVGVVLDTAGSERAILF
                     GEGGDGGGVCALFAASFPQRSTALIWWDATPRSAWAPDYPWGMTEEQFAASEAFMEEA
                     WGSNEGIAELVRQVGGASIADDTEAKQWWAKLYRYAATPAAAVALGRMWFETDVRNIL
                     PAIHVPSLVVSSHHREDFANTASSIPGAVLAEVPSPDRPPWRGDQDALFSVLGRFLDS
                     VRDERTAFDRVLATVLFTDIVGSTDKVSSLGDRVWKELVEKHHALVRGLLIRFGGREV
                     DTAGDGFFATFDGPARAIRCATAIAESVRRFGLEVRTGIHTGEVETIAGKTGGIAVVI
                     GARIAGIAAPSEVLVSATVKDLVAGSGLAFEPRGEHELKGVPDHWRLYRVMREPSAGP
                     "
     gene            4047..4577
                     /locus_tag="VF195_09995"
     CDS             4047..4577
                     /locus_tag="VF195_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581188.1"
                     /translation="MTSILIRANGRVDFLTRNEDDDVHFHRCVGGPPQIIALPGGPDG
                     ETTWSCAVSPEATSFAPKPLSITAEGSISGISSADGGGYLIGVFLADSVPAGPPPATL
                     DFTKGRDFASLSPELAQTFFIGDGRTTAGRRQRFRAPPGATRLYLGLADAWMFQGPPG
                     FYADNSGSFKVAVRFH"
BASE COUNT          855 a         1654 c         1495 g          762 t
ORIGIN      
        1 cccgctcggc gcccgagcgg cgacccaaag cgtcccaagc ccgagcaccg tccccacgag
       61 ccaggcgatc ccgccgagga acgggacgag ggcgagcacc cgcaatgccc cccatccggc
      121 gaggaacgcg aggtaccgag aagtcggctc cttgacgacg agccggccga gggcgagacc
      181 gccgaccacg tagccgatcg agtacacgag cccgagcccg aagagtagga acagccccaa
      241 ggggattcca acgatcgtga cgagcagcag gacggccacg atcggcagca ggacgaacca
      301 caagagcccg aacccgatcg tcgcccccaa gcggtctcgg agagcccgga tgctcgcggg
      361 atcgagtccc cgagccagca tgagtagaac cagaccgagc acgagcgttg acacggtgta
      421 cgcgagccac caggccaggc gaccgacgaa cgtgatgtcc cagtagtccc accgcttctg
      481 aaggtcatcg atgcttcctt cgacggtggc cccgtcctcg atctgcggag tctgtcggct
      541 gacgacgtct ccccccaccc gcgcgcccga gcgcagcacc acgctgccgt tgaacacgac
      601 gacgtcctca ccgaccgtcc cggtgatctc ggtccggccg ttgaatacga cgagtgtttc
      661 gctgaccgtg ccgtcgatca gcacgtcccc gttgaagacg acggcggtct tggccgtctc
      721 gccctcgggc accacgagac ctcccgtcag gatcacgtgg tcgtcgctgt ccgggccgtc
      781 gtcatcctga gccaccgccc cgggcgcgag gatcaccaag ctgaggccga ggatggctgc
      841 gagaccgacc gatctacgga accaccaccg catgcccttg aactgccagt cgaagactcg
      901 tgcgatcgtt cgcggattct gagggtccac ctggcgctcc tctccaagag gccaacgggt
      961 tgctctattt ggtaactcag atgcgcacca cggtcaaatc gaccgtgggg cgacctgggg
     1021 aggttcggga atggcttccg gaagtcgtcc aggggagcag ccgcatcaag gacgagatcg
     1081 gcctggagtc ctcgaaatcc ggcgcaggaa aatccgatct gcacctggcg gcagagctcg
     1141 gcggcaagta cgaggcccta gggccgaaaa agggttccag gaatcgggtc tacactcgag
     1201 aaccgcccgg aagtcgcgcg tccacccaag gtgacgttgc gtcgacccga agacccagag
     1261 gcgcaacgag gcgctcgatc acgtggacga ccgcctcctc cccctcaaac agacggtccg
     1321 ccaccctctc gatccgccta ttacatgcac ttctcgttat tagggcgctg acctctgact
     1381 cccgtcccga gcaaccctcg gaccctcgtt agccaggtga aggaagcgac acggctcgac
     1441 cccgttcagg ccgacatcgc cgattggaac cgggagattg ccgacgctgc ggccggcaag
     1501 aacattgcac atccgcctgt tgacgtggac gctttcgtcc tggagaccaa gcaggtaatg
     1561 gtcgaggaga accgcgccga ggccaagcag gcggccctgg gagggacggt tggacgtcaa
     1621 cgtggtcctc gatcccgtgc ccgtccgggt tgacacaagg atgagatgtg gcatctggcg
     1681 atcaagggtc accgatgcga tcggcgcact cgaacggtca tccgaacccg ccatccaccc
     1741 accgctcgcg ggtgaggacg tagaagtcga actcctgtcc ctcgtatcgg atcgtgccat
     1801 ctgtctcgaa cccgagccgc tccgcgaccg tgcgcatggc cacgttgtcg gggaccgtgg
     1861 gcatgtgtat ttgcgtggct tccgcttccg agaacagcca tccgacgagc agcatgaccg
     1921 cctcggtgcc gtatccctta ccccgcatgg agggctcgtc gatgacgatc ccgatctcga
     1981 aggcgccgga tggaagcgtt cgatccgggg gaacgtaggt ctggatcccg ccgatcatcc
     2041 gaccggcgac ctcaatggca agatccagcg ctccgccctc catgatcccc gagcgctcga
     2101 accggactcg aagcgcaaca cgcgtcggca tgatcggctg cactgacgga tccatctcga
     2161 gtcggttcgc ccactcctga tcgaactcgt ccatcgagag cgggcggagg atcacgagct
     2221 ccccctcgag ccggatcctg ttcacctcaa ggtcccgcgg aaggctcgcg catcacccgg
     2281 tacagccgcc agtgatccgg gacgcccttc agctcgtgct cgcctcgagg ctcgaacgct
     2341 agccccgacc ccgcgaccag atccttcacg gttgccgaca cgaggacctc cgagggcgcc
     2401 gcgatgcccg cgatccgggc tccgatcacg acggcgatcc ctcctgtctt cccggcgatc
     2461 gtttcgacct cgccggtatg gatgccggtg cggacttcga gcccgaaccg ccgcacgctc
     2521 tcggcgatgg cggtcgcgca gcggatggcg cgggcaggcc cgtcgaacgt ggcgaagaac
     2581 ccgtcgccgg ctgtatcgac ttcccggcca ccaaagcgga tgagcaggcc gcgaaccagc
     2641 gcgtggtgct tctcgacgag ctccttccac acgcgatcgc cgaggctcga aaccttgtcc
     2701 gtcgagccga cgatgtcggt gaagagcacc gtggcaagga cccggtcgaa cgccgtcctc
     2761 tcgtcgcgga ccgaatcgag gaaccgcccg agcacggaga agagcgcatc ttgatcaccg
     2821 cgccatggcg gccggtcggg cgatgggacc tcagccagca ccgcaccggg gatcgagctg
     2881 gcggtgttcg cgaagtcctc ccgatggtga ctagagacga cgagcgaggg gacgtggatg
     2941 gccggaagga tgtttcgcac gtccgtctcg aaccacatcc gcccgagcgc tacggcggcg
     3001 gctggggtcg ccgcataacg gtagagcttc gcccaccact gcttggcctc ggtgtcatcg
     3061 gcgatcgatg cgccaccgac ctgacgaacc agctcggcta tgccttcgtt ggatccccag
     3121 gcctcctcca tgaaggcttc ggacgctgcg aactgctctt cggtcatgcc ccacgggtaa
     3181 tcgggggccc acgcgcttcg aggggtggcg tcccaccaga tgagggcggt cgatcgctgg
     3241 gggaacgatg ccgcgaagag cgcgcagacg ccacctccat cgcctccctc gccgaagagg
     3301 atggctcgct cggagccggc agtgtcgagc accacaccaa cgtcgtccat catcgtctcg
     3361 aggccgggca gacgggagac gcgatccgac agaccggtcc ctcgcttatc gaaccagatg
     3421 acgcgggcga acgtcgccag ccgccgcagg aacctggcgg cgggtgggta ctcccagaac
     3481 acctcgaggt gggtcgcggc tcccgggacc cacaggagat cttggtcacc ctcgccgaac
     3541 gcctggtacg cgaggtaggt cccgtcactc gccttcgtgt atcgggtttg gggaatcacg
     3601 cccgtagtct gccccgttcg cggcccgcac gcctcgaacg ggtgctgcgg cgtttacagc
     3661 gagcccgcgg ttaggcagaa gccgggtgac ggacgacacc gcgcaccgcc atgcctcgct
     3721 ctagcgtgac ggccgtgcag catcgcgaga tgagcgtgtc cagtaaaggt ggacactcga
     3781 gcgtccatcg gccgctcgcg gcggtcgcga ttgcgtgtct cctgacggtg ggctgcaccg
     3841 gcaagagcga tcaccaggtg cccccctcga acgctcccac gatcagcaca aacgcggagc
     3901 cggaacccgt cgagccctgt ggatccgtag tcaggcttga ggtgcccggt acctcgaaca
     3961 tcttcggcgc gggcatcgcc gatccgccgg ctcccgcggg tggtggagct gggacagcac
     4021 cgccctgtgt gtccctccca tcgacggtga ccagcatcct catccgagct aacgggaggg
     4081 tggacttcct cacacgaaat gaggacgacg acgtgcactt ccatcgttgc gtcggtggac
     4141 cgcctcagat catcgcgctg ccggggggac cggacggtga gacgacgtgg tcgtgcgccg
     4201 tctctcccga ggcgacgtcg ttcgccccca agccgttgtc gatcaccgcc gagggctcga
     4261 tctcggggat ctcgtccgcc gacggcggcg ggtacctcat cggcgtcttc ctcgccgata
     4321 gcgtgcccgc cggaccaccc cctgcgaccc tcgacttcac gaagggtcgc gacttcgctt
     4381 cgctttcacc cgagctggcc cagaccttct ttatcggcga tggtcggacc accgccggta
     4441 ggcggcagcg gttccgtgcg ccaccaggag ccacgcggtt gtacttgggt ctcgcggacg
     4501 cctggatgtt ccagggtccg ccgggcttct acgcggataa cagcggatcg ttcaaggtgg
     4561 ccgtccgctt ccattagcgc caaggcttct cgcgcggcca gaccttgccc cacgccgcgc
     4621 gccttgtcga tcccacaagg cgactacgca ccttggaatc gtcctgggct catcggtcaa
     4681 cctctcgcaa catcccgaca agcaccgctc ggagcgtcca gtcctcctcg cagtcgttct
     4741 gccttcaggt tgcctcgtgt ctttgc
//
LOCUS       DASUYD010000179        44091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_330176, whole genome shotgun sequence.
ACCESSION   DASUYD010000179 DASUYD010000000
VERSION     DASUYD010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 44091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44091
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330176"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1111)
                     /gene="gap"
                     /locus_tag="VF195_10000"
     CDS             complement(104..1111)
                     /gene="gap"
                     /locus_tag="VF195_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX6581189.1"
                     /translation="MSVKVGINGFGRIGRNFLRAVRLTGADVDFVGVNDITDAKTLAH
                     LLKYDSVLGRLDAEVAVSDAGLSVDGDELRVFAERDPAQLPWKEVGAEIVIESTGLFT
                     DREKAAAHLEAGAQKVVISAPAKGEDITIVMGVNDDVYDPTTHHVISNASCTTNCVVP
                     LAKVLEDSFGIDRALMTTVHAYTNDQNLLDLPHKDLRRARAAAINIVPASTGAAKATS
                     LALPQLKGKMDGIAMRVPVPDGSITDLVAIVNRDVTVEEVNDAYRTASETDRLKGKLV
                     YTEDPIVSSDIVGSPASCTFDSLSTMAMGNMVKVMGWYDNEWGYSNRLVDLVNLVASK
                     L"
     gene            1204..1482
                     /locus_tag="VF195_10005"
     CDS             1204..1482
                     /locus_tag="VF195_10005"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6581190.1"
                     /translation="MGDGVDARNRASADRLAALGARLSDDELRTEIDPPWTAAGLFAH
                     IAFWDRFVLERWRLAAERGERTPMAVDGGLMDRINDASLRQWMSIPSR"
     gene            1479..1652
                     /locus_tag="VF195_10010"
     CDS             1479..1652
                     /locus_tag="VF195_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581191.1"
                     /translation="MTVEECLSSAAALDAFVEDVADDVRGELAAEGRERLVDRSLHRA
                     DHLGTLEAAFPGG"
     gene            complement(1659..2555)
                     /gene="rapZ"
                     /locus_tag="VF195_10015"
     CDS             complement(1659..2555)
                     /gene="rapZ"
                     /locus_tag="VF195_10015"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX6581192.1"
                     /translation="MARAVKRSARETAAPGFLLITGLSGAGRSEAARSLEDLGYFVVD
                     NLPPALLPKMAELAASPGGPGRVAIVVDARGGVFFGELSKALEELREQRVPSRIIFLD
                     ASDEDLVNRYEATRRRHPLAPGDRVGEGIRKERLMMESLRADADLVIDTTGLTPHDLR
                     DRMRETFADTPPEAGLLVSIVSFGFKYGSPRDADLVMDVRFLPNPHWVEELRPLPGSN
                     EKVRQYVEGQEEYGSFMTRLEGLLDVVVPGYVDEGKSYLTIAIGCTGGRHRSVVVADD
                     LADWFREHGHRVSVSHRDVDRD"
     gene            complement(2555..4432)
                     /gene="uvrC"
                     /locus_tag="VF195_10020"
     CDS             complement(2555..4432)
                     /gene="uvrC"
                     /locus_tag="VF195_10020"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX6581193.1"
                     /translation="MSFTKPASSTIPDAPGAYLFRDEDGRVVYVGKAGSLRKRLANYW
                     SRPLHPRTEAMVDAAASVEWIVASGEVDALMLEFNLIQQYLPRFNIRYRDDKSYPYLA
                     LTVGERWPRAQVLRGTKRKKVRYFGPYGHAWAIRDTLDALTRVFPIRTCTNHFFDQRA
                     RAGRPCLYYDIGRCSGPCVPAATGITEEAYRSDVDALGDFLAGNSKPVLRRLEEQMAE
                     ASGREEYEQAARFRDQLQAARRALESQEMVLVQPESLDVVGLAEDDLEAAFQVFTVRG
                     GRVLGRKGWIVDRVEDLDRPELVASFLRQLYMEREDVPPRILVPTEPAEREVLEAWLS
                     ARRGSHVTIAVPARGAKRRLMEVVGSNAGEAFHRHKLRRASDFGARSRALSELADQLG
                     LEQAPLRIECYDISNLGPSDTVGSMVVFEDGLPKRADYRRFEIKGVPGQDDLASMEEM
                     LTRRFSRLLKEKAEPPGGRRRFSYPPALVVVDGGRGQLSVATRVLEDLGLHIPHIGLA
                     KRLEEIYFPDRPDPLSIARGSEALFVLQHIRDEAHRFAITYHRQRRDRRALSSPLDEI
                     PGVGPSRKRALMNRFGSLTKIRQAGAVELAATPGVGPRLADVIHRRLHPVSVTDRRES
                     A"
     gene            complement(4481..6133)
                     /locus_tag="VF195_10025"
     CDS             complement(4481..6133)
                     /locus_tag="VF195_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581194.1"
                     /translation="MSRPSPRSLALALVVLAAACGSIPPSEVEAPQGRQFIPMVPDSI
                     DDVGLTPSVAVDSQGLPTISYYGFPAQLEEGEIPIARPVGSPFLTTEDGDDAGAVLMV
                     SFNPDQIWNRGAIAQPRDTPSGVPVPFEPAAEPSLSSLTKPRAAGTDVAIAGSDVHAV
                     WTTDRGVWYGIGPTPFEIGAVEETPEASAPSVVVDGTGAPIVAYTIAGAQPEVRVAER
                     VGEDWRVTTVATLSRCGEGCPPATQLALLGGEPLVVTADPLSGEVIAARRQGGAWTTE
                     VVATDAAGGASVSSAGDTAAIAYYTETGVALATGRPGSWSAEEVAALATGGGDRSADV
                     PTPPSTGVAVDQQGTVWVAWQNGDGIHLASQADGPIEEVELPDTSSGVNPSLAITEDG
                     SSVYLAWYDANEGDLRLGTFGEISDLLIAAPTPLPSPAPAGPPEDCGGDGEPVLEVTA
                     PTTAFEPTCLVAPAEEGFTITFNNEDEGVTHNLAIRPDADGENITATDLKPGTYTDEL
                     PVDPQEEGDLYFLCEAHPDVMFGTLAVVAGGGGNGGGGGGGG"
     gene            complement(6178..9033)
                     /gene="uvrA"
                     /locus_tag="VF195_10030"
     CDS             complement(6178..9033)
                     /gene="uvrA"
                     /locus_tag="VF195_10030"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX6581195.1"
                     /translation="MGSERLVIHGAREHNLKNVTLDLPRDQLIVFTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSSYARQFLGQMEKPDVDFIEGLSPAISIDQKSTSRNPRSTVGT
                     ITEVYDYLRVLFARIGHPHCYNCGRPIGRQTPDQIVDQVLQLPEGTRFQVLAPVIRGR
                     KGEYEKLLQELARKGFPRARIDGEVRELSESIRLPKNFKHTIEVVVDRLVAKQGIRRR
                     VADSIETALELAEGIASIAVTTHEGHEELQTFSQKLACTFCGLSFDELAPRNFSFNSP
                     YGACSTCDGLGTRLEVDADLVVPDPALSINEGALAPWASNTLEYWYRVLEAVGEAHGF
                     SLDTPWKKLPKRARDVVLFGSDEEIYVRYKNRYGRQRSYYTSYEGVLPNIERRHEETD
                     SDASREKLEQFMRETPCRSCTGARLRPETLAVTVDGLHISALTDLSIRKTLSFLESME
                     LSEREEMIAERLLKEIRARLGFLVDVGLDYLTLSRPAGTLAGGEAQRIRLATQIGSGL
                     VGVLYILDEPSIGLHQRDNRRLLDTLIRLRDLGNTLIVVEHDEATIIEADHIVDIGPL
                     AGEHGGRIVYSGDLKGLLGSDESLTGAYLSGRRSIPLPSSRRAPKRWLRLEGVRQHNL
                     KDITVKIPLGVMVCVTGVSGSGKSTLVQDVLLRALMQKVYRSRVLPGKHRRMVGWEDV
                     DKVIDIDQSPIGRTPRSNPATYTGVWDHVRKLFAQVPEARLRGYQPGRFSFNVRGGRC
                     ENCAGDGQIKIEMHFLPDVYVTCEVCKGRRYNRETLEVKYKGRTVSDVLEMSVDEALE
                     FFQNIPPIKRHMQTLSDVGLGYIKLGQPAPTLSGGEAQRIKLSSELHKRATGNTVYIL
                     DEPTTGLHFEDIRKLLQVLQRLVATGNTVLVIEHNLDVVKTADWLIDLGPEGGDQGGR
                     IVAEGPPEAIAANADSHTGRFLEEVLGVLPLGVGASA"
     gene            9165..11144
                     /locus_tag="VF195_10035"
     CDS             9165..11144
                     /locus_tag="VF195_10035"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6581196.1"
                     /translation="MDRDLRSSPLYQEIEEHFRKVYEPGFGKVSEASDPRPSPDGRWV
                     AFTGSIWEELEGTPKTRVCLALAGAEDGSARQVTAGPERDAGARWSPDGRRLSFLSDR
                     RGKGRMQLYALDADALGEAEALPEVEGTIEEHAWSPDGSRILVRTAGMHADGAGADGS
                     GALAGEKDLPDWVPAVDSWEDRQVWRRLWVIEASSGEVRALSREGLNVWEAEWCGDGA
                     VVAVASEDPGESAWYAAPVVLIDTETGEDRVLATSDVQFGLPVGSPDGSRVAAVEAPC
                     SDRQLVSGRAVVVDVQSGERRVVEMEDADITWLGWRADGVLSYAALRRGDTVFGDIDP
                     ASGNRTETWSTSEAVGAWAPYAAPLGADGFAFIRQGFDRPIEVAVVEGGVDRTVASFR
                     HEGHDHARGSVGRAERVTWNAPDGLEIDGFLLTPPGDGPHPLILHVHGGPVSSYMESF
                     PRPSFAWLVSRGYAILLPNPRGSTGRGRAFLEAVIGDMGGADARDDLAGVDALVERGV
                     ADPGRIGVTGGSYGGFMSCWLPVVDQRFKASVAVSPVTDFYSQHWNSNIGAWDAWFLG
                     GEPQDDAPQYRERSPVFFADRVTTPTLLTAGTEDRCTPPGQAIEFSRALRARGVPVEV
                     AIYPGEGHGVRKLPAYLDLVARTTAWFERFMPARG"
     gene            11144..12289
                     /locus_tag="VF195_10040"
     CDS             11144..12289
                     /locus_tag="VF195_10040"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX6581197.1"
                     /translation="MDLTAWRDEFPVLERKTYLISASLGPVSRLSRAFLEEYLDVWGS
                     KGAPDLVWFEDIFPRMAALKRSFSALAGCDPDEVALTTNISIALSTIASCLDLSGERR
                     TVVLSELDFPTDGQVWIAWARKAGAEIRWLRSPDGLTIPLEEYDRAIDERTALVMVNR
                     VLYRSSAIVDAKALCTIARDRGALSFVDDYHGLGIVPLDLHDLGCDLYTAGVLKWMLG
                     GPGLVFLYARRDLLPSLEPAITGWFGQREPFAFDAERLDYHPTARRLEHGTPPAPVFF
                     IAQGGIDIISKVGPAAIRARQGELTDHVIARADGAGLEVRTPRDREARGGVVNVKVGP
                     EAEKICHELLARDVCTDFRGDGLRISPHFFNTGEDIDRCFDELRQLV"
     gene            complement(12294..13367)
                     /locus_tag="VF195_10045"
     CDS             complement(12294..13367)
                     /locus_tag="VF195_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HEX6581198.1"
                     /translation="MVQTDTTTWQGVTQKELGQAARNHGMQLEGLRYDVTPIGMHYLV
                     IHWDIPAADETAWTVDVDGLVDRPLTLSMDDIRSRPAVTRPVTMECAGNGRALMPPRA
                     ESQPWLLEAVGNAEWMGTPLPPILEDAGLRPEALELVFTGADHGIQGDVEQDYQWGFS
                     LADIVKNDVLLAYELNGQPLPPQHGYPIRLLVPDWYGMCSVKWLRRITAVADPFDGFQ
                     LSAYQRRQEPEGPGERVTRKAVRALMLPPGFSDFPARQRFVDAGGHELIGRAWSGWGH
                     IDRVEVSIDGGATWSDAELGPEPESSSSWRSWSFDWDARPGERELLVRATDEAGNVQP
                     MDPPWNYGGFMNNMPQRVPVVVR"
     gene            complement(13414..14445)
                     /locus_tag="VF195_10050"
     CDS             complement(13414..14445)
                     /locus_tag="VF195_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6581199.1"
                     /translation="MNRLSVRAPAGWLGPGGFLEDAQIACEGGRITYAGAATGVEASD
                     REIVVDGFLMPAVADRHVHIELSDPAAVLAGGVTAVRDLAWPVERIFSLADASEMPSF
                     EGPLIKAAGPMLTGPGGYPTQDPWAPPGTGREIKGTDDATAAVEELARLGAAAIKVAL
                     NTDAGPTPTDGELSSICDAAHAADLPVTAHAQGKGQVERALGAGVDELAHTPWTRLSD
                     QVIGSAAAQMRIVSTLDISSFGRDTPEIRTALDNLRRFHVAGGTIVYGTDLGNGSIPP
                     GIHTREALLMAEAGLEPEEVLEAMIRAPLAPDAPADLIALRQSPLEDLRALDDVVLVI
                     RAGRVVAGG"
     gene            complement(14442..16466)
                     /gene="uvrB"
                     /locus_tag="VF195_10055"
     CDS             complement(14442..16466)
                     /gene="uvrB"
                     /locus_tag="VF195_10055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX6581200.1"
                     /translation="MPPFKITSEFRPSGDQPEAIEQLVDGVQAGQEHVTLLGATGTGK
                     TFTVAHLVERVQRPTLVIAPNKSLAAQLANEFREVFPENAVEYFVSYYDYYQPEAYVP
                     QTDTYIEKDSSINDEIERLRHSATAALLSRRDVLIVASVSCIYGLGSPEQYEGQILRT
                     VRGTDLPMEFAMQRLVDIQYKRNNATRARGTFRVQGDTLEVFPSYEQVGYRIEWWGET
                     IEKISRFDPLTGELLTDAIVDLTTFPASHYVSSEDRMQQAIVTIEEELQARLAELESQ
                     SKLLEAERLRMRTTYDLEMLREVGFCSGIENYSRHLDGRAAGSAPYTLLDYFPDDYLV
                     VIDESHVTVPQLHGMYEGDISRKSTLVDFGFRLPSAIDNRPLRFEEFQERVHQIVYMS
                     ATPGPWELKVSDRIVEQIVRPTGLIDPEVEIRPTKGQVDDLIEEIRRRAEAEQRVLVT
                     TLTKKMAEDLTDYLIEMGIRVRYLHSEVDTIQRIEIVRDLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRSDTSLIQTIGRAARNVDGKVLMYADAVTDSMKTAISET
                     QRRRMIQAAYNTEHGIDPTTIRRRVHDILLSISEDPAKRATSPRKERRQRLARPEMPQ
                     EELERLLLELEEEMHQAAKDLRFEYAARLRDEVVDLRKELKALRAAGVGA"
     gene            complement(16506..17252)
                     /locus_tag="VF195_10060"
     CDS             complement(16506..17252)
                     /locus_tag="VF195_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019060487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6581201.1"
                     /translation="MTEAVRIQGVRKAFGELEVLRGIDLVVEDHEVVGLIGASGSGKS
                     TLLRCIDLLERVDAGRIWIEGVEITASRVDENAVRRRVGIVFQAFNLFPHMSVIRNVM
                     LAQEKALGRSQAEARPKAVELLERFGLADKADDYPDRLSGGQQQRVAIARALALDPDV
                     MLLDEVTSALDPVLVAEVLDTIRELAAAGMTMVIATHEMGFARDVANRVAFLEEGRIL
                     EEGPPERMFSEPREEATRRFLDRIIAAGRM"
     gene            complement(17249..18121)
                     /locus_tag="VF195_10065"
     CDS             complement(17249..18121)
                     /locus_tag="VF195_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017884839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6581202.1"
                     /translation="MTEPMEALPGHDRRARMLGNRGVLISTASTILFFGVLAVIVVSA
                     PGFEALRESFFNLEAMKESFLGVPEEGLPGIGRALLLNIEVFLVAEVLILILALVVAV
                     VRGLRGPVFFPFRLIAIVYTDLFRGTPLLLVIYLFGFGLPALGIRGLSTQSEVVYATG
                     ALVLSYSAYVAEVYRAGIESVHPSQIAAARSLGLSRLQSLRFVVLPQAIRRVIPPLLN
                     DFVSLQKDTALLAVLGVVEAARAAQIYSAYRFNYSSFVVAAFLFVLMTIPLARLTDWL
                     IERDRRRRQAGVTT"
     gene            complement(18138..19013)
                     /locus_tag="VF195_10070"
     CDS             complement(18138..19013)
                     /locus_tag="VF195_10070"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581203.1"
                     /translation="MRRWILALGVLALVAGACSEGTVSGDDGTPSSASPSEEMTASAC
                     AESHSADFFEEGTLTVGTDFDFMFPPWILNRKPESGRGFESAVAYEVATRMGFSGDDV
                     HWVPIPFNRSYAPGPKDFDFDINNISVTPERDQAVDFSDGYYDLTQALMVLKGSSIEH
                     ATTMEEIKDAIFGAQIGTTSLAFINTVIQPTNDAKVFDSTSDAKAALRNGTVDGLVLD
                     LPTAYFEAYINTPNGALVGQFPSAGEHLGLLFEEGSPLVACANLAIAEMTADGTLEQL
                     QNKWLADYLAVPVIQ"
     gene            complement(19064..19858)
                     /locus_tag="VF195_10075"
     CDS             complement(19064..19858)
                     /locus_tag="VF195_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581204.1"
                     /translation="MSVVPQVGRQFRFTNKAFWRNPASAFFTFAFPLMFLVIFTALLG
                     QGQVELQRFELDTTTYYVGAMAAFGVVSACFTNIAIQTTFNRDAGILKRLRGTPLPAG
                     AYMAARVLHAMVVGAILVVITLVFGRIVYDSPLPSGGPLLEFTVTFLVGSLSFAALAL
                     ALTAVIPNADAAPPIVNAAILPLLFLSGIFIPLDDEAPQWITTVADVFPVKHFADAMR
                     AGFLGNIEVAGQRVFVFDWLDVAIVAAWGIAGLILAVRFFSWEPRR"
     gene            complement(19855..20769)
                     /locus_tag="VF195_10080"
     CDS             complement(19855..20769)
                     /locus_tag="VF195_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018543772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581205.1"
                     /translation="MTTERVIDVRGLRKSYGDVEAVAGVDLHVDRGEVFALLGPNGAG
                     KTTAVEILEGYRDRSDGEVSVLGHDPARTDADLKRRIGIVLQSTGVDPYLTVRETVAL
                     YAGFYPSPRDVDEVVDLVGLAEKRNTRVSKLSGGQQRRLDVAVALAGDPELLFLDEPT
                     TGFDPNARRNAWEIVKNLTAIGKTIFLTTHFMDEAQYLANRVAVITSGSIVAEGTPAT
                     LGGRDQMRTRVRFLLPAGGPTPPGHATTARRPDGTIDIRTENPTRVVHELTAWALEHD
                     LVFDLLEVTQPSLEDVYLELTGGEEGRE"
     gene            complement(20774..21145)
                     /locus_tag="VF195_10085"
     CDS             complement(20774..21145)
                     /locus_tag="VF195_10085"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HEX6581206.1"
                     /translation="MTEERLFSIADADAELEELRARLPRLREARQRLIDTGERITSAV
                     ELDGGGTEGSDWFRAQETLKRELLWLAGRGILLRDPDAGLVDFPAERDGERVFLCWRL
                     GEERVGWYHSERSGLSSRKPL"
     gene            complement(21142..21846)
                     /locus_tag="VF195_10090"
     CDS             complement(21142..21846)
                     /locus_tag="VF195_10090"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6581207.1"
                     /translation="MRFRAVFFDVGETLVHVDPSFADLFVTVLADAGHERSAQDVREA
                     SAHVYARFSEAARDGSMWTTSPERSRAFWTSVYERMLDELGVADGDGLATSLHGEFTR
                     MENYVLFDDVLPTLATLRETGVVLGVVSNFEAWLEDWFGVHELLELFPVRVISGIEGV
                     EKPDERIFRLALDRASVSAEESAYVGDNPEFDVDPPAALGMFPVLVDRRGRFPHHAGA
                     RITDLRELPSILGATA"
     gene            complement(21915..22517)
                     /gene="coaE"
                     /locus_tag="VF195_10095"
     CDS             complement(21915..22517)
                     /gene="coaE"
                     /locus_tag="VF195_10095"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019984078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEX6581208.1"
                     /translation="MLLVGLTGGIGSGKSTVARLLERRGAVVIDADHLAREAVARGTT
                     GFDRVVDTFGPGVLKPDGDLDRVRLAAIIFADPERRAALEAIVHPEVAKRFGERVEEL
                     RDSGRVVVYVSPLLVELGLAPAFDVVVIVTASPHLRVSRVASDRDLSPEDVRARMAAQ
                     ATDEQRMEVADVLVDNDGSLAELEPQIDRLWSDLEARARV"
     gene            complement(22528..24132)
                     /gene="rpsA"
                     /locus_tag="VF195_10100"
     CDS             complement(22528..24132)
                     /gene="rpsA"
                     /locus_tag="VF195_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007928412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEX6581209.1"
                     /translation="MSETETTTESVPDGTVETSEPIVDEEAVSEIDTEAAGDMAPPAT
                     PTDEVPLVATLEPEGEDYTPEELVRAMEASLRDFKDGDIMEGVVVKIDRDEILLDIGY
                     KSEGVIPSKELSIRHDVDPNEIVKVGDRIEALVLQKEDKEGRLILSKKRAQYERAWGR
                     IEETMASGQTIKGPVIEVVKGGLILDIGLRGFLPASLVDLRRVRDLQPYIGTELECKI
                     IELDRNRNNVVLSRRAFLEESQSEGRKKFLENLEKGERRKGTVSSIVNFGAFVDLGGV
                     DGLVHVSELSWKHVDHPSEVVTVGQEVEVQVLDVELDRERVSLSLKATQEDPWKEFER
                     LHKAGEVIAGQVTKLVPFGAFVRVAQGIEGLVHISELSHEHVDTPESVLSVGDEVQVK
                     VVDVDVSRRRVSLSMRQVTPAPAKTTEIEVEHEQPERAAEPLVQTVQIPQAVLDAVAE
                     AETDEPAATAAPTVEPAAVEPTAEPEAAPEPAPEAAPDEPADAAAVAPDVAAQPEDAT
                     EEPASDEEVSLEAILEDLKRREGRSE"
     gene            complement(24272..26938)
                     /gene="polA"
                     /locus_tag="VF195_10105"
     CDS             complement(24272..26938)
                     /gene="polA"
                     /locus_tag="VF195_10105"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEX6581210.1"
                     /translation="MAPPSDPTDDQPRRLFILDGHSLAYRAFFALPSTLATTTGQVTN
                     AVYGFTSMLIKLLAEERPDLIAVAFDVGKPTARLAMDADYKAGRRETPEDFRPQLGLI
                     QEVVEALRIRMLLVTDHEADDAIGTLAIRAAEQGIEATIVTADRDFFQLVRPGLRVLF
                     NRRGISDIALMDEAAVEERYGIPPSKYLEYVALKGDTSDNIPGVPGVGDKTAAKLVQQ
                     FGSVEELLGRTAELKGKLKENIEAAGDRLALNKNLARIVTDVDLDFTPDDAVMGEWDV
                     EEVRRLFTSLEFRSLFDRLMELAKDVKPKVEVAELDLRRVEPEDLAGILADGPVGVAI
                     HEEDDVLVGLAVSAGGAQAAYAALPGPGPIADRLADASTRKWAHDAKALERVTLRAGA
                     VLEGVAFDTMLAGYLLEPGTADYPLAALCERYLGTDLFGVEGEAATETEMQLFTEEPW
                     RHVASEAAAVSLLAPVLEEQIDRQGLRPLLEDVELPLASVLARMEARGVRLDVPYLED
                     MGGSVRDRMATLHAEVFRHAGQEFNLNSPPQLRAILYDQLGLQPGKKTPKGELSTDAS
                     VLEKLRDAHPIVDALLSWRELDKLNSTYLEALPRLVDAKDGRLHTSFNQTVAATGRLS
                     SSNPNLQNIPIRSELGRQIRRAFIPGAYDQVLLVADYSQIELRILAHLSGDAGLREAF
                     DSGLDIHAATAAKVFDLPPEHVDAEMRRRAKAVNYGLAYGMNAWGLATRLGITADEAQ
                     EFIDAYFDGFPAIKDYLQNQVDRATVEGFTETLLGRRRYIPELQAANPRVRDLGRRQA
                     LNAPIQGSASDVFKVGMIGVDGALRERPELDCHMLLTVHDELVFEVAKDRAEEAADLI
                     RDRMEGAVELVVDLRADIGVGPNWSEAAPEGH"
     gene            27133..28026
                     /gene="efeU"
                     /locus_tag="VF195_10110"
     CDS             27133..28026
                     /gene="efeU"
                     /locus_tag="VF195_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEX6581211.1"
                     /translation="MGATFVIALREGIEAALIVSIVLAYLKQLGASDRARLVWWGTGL
                     AVALSAAVGTGIFVAGAEFEGAAEEVFEGLVTLAAVGVLTWMIFWMRRQGSRIRSELQ
                     EKVDTAIVSGGLALAGLAFFAVLREGIETALFLFAAAKGTAVEGTAVAPATQVTGAVI
                     GLAIAVVLGVLLYRGGIRMNLRSFFRVTGLILIVVAAGLFAYSLHELQEAGWLPVLEA
                     HAFDLSASLPDDAGAGAILRGLVGYNADPTWLEVVGWAAYLVVVGGLFLRRPGVPLAN
                     SRPEHETSVTGATHVETDASR"
     gene            complement(28335..29102)
                     /locus_tag="VF195_10115"
     CDS             complement(28335..29102)
                     /locus_tag="VF195_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015336823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6581212.1"
                     /translation="MSGRDGQPVVRVENLHKSFGNLEVLRGIDIEVDAGEVIVVFGRS
                     GSGKSTLLRCINFLEEPTEGTIEVSGLRIEGGHRIRAKREQIRQLRMRCGMVFQQFNL
                     FPHMTALQNVMEGPATVKGIEEGSASRVAMEFLDRVGLSDKAGEYPIRLSGGQQQRVA
                     IARALAMEPDVMLFDEPTSALDPELIGEVLGVMKALAMEMHMTMMVVTHEMGFAREVA
                     SRMAFFHEGAILEEGTPRQIFEETRSPETRRFLDAVL"
     gene            complement(29099..30169)
                     /locus_tag="VF195_10120"
     CDS             complement(29099..30169)
                     /locus_tag="VF195_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6581213.1"
                     /translation="MDTGEAGAAARPPLAPIQRRRDRLALASAGVSFRVKVAAVWLVI
                     LALLGFLFWASSFDNQWIREHIGFIARGLVFTLGMAAGGIILAVVLALLGALGRLSRN
                     PVAYGVSGFYTSFFRGTPLIVQLFLFYLALPRLGINLTARFPDLPGGLVDILTRPAFQ
                     VGLLVLGLNYGAYMTEIFRAGIQSVSHGQSEAAEALGMTYRQKMRRVVLPQAFRVIIP
                     PTGNEFIAMMKDTALVSVLGQSIESMELFRRAQLIGNADVRPMEALITAAGLYWALTA
                     IFTYFQSRLERRVNKGYVRAVAGPKVRERRKVIVTGAGGGGEHGAAAGYVVDAPEEEV
                     GLVGHAHGGALPTVPEPDEDPP"
     gene            complement(30191..31114)
                     /locus_tag="VF195_10125"
     CDS             complement(30191..31114)
                     /locus_tag="VF195_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6581214.1"
                     /translation="MKVGFRRIAVLVGVLALIAAGCGEDPAPPAGGNGDDPADETVCA
                     TYDKEAGDLLAKICTEGVIRVSTDPAYPPASELNPETGEYEGFDIDTANEIAARLGVK
                     VEWETPDWEVLTAGSWNDRWDMSVGSMTPTNDRQEVLNFTEPYAFVPAVVVVHEENTT
                     ISDLTTDLDGKRIGVCADCTYQFFIEKTLEISGFEFDFVIDDATVAGYDTDTTALQDL
                     ALGDGTRVDAVMTASSIAQEFVEAGNPVKIVGEPLFGEPLSVAFDKSSELDGTTLVET
                     VDGIVADMHEDGTLSSFSETWYPGADVSEVA"
     gene            complement(31206..32423)
                     /locus_tag="VF195_10130"
     CDS             complement(31206..32423)
                     /locus_tag="VF195_10130"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581215.1"
                     /translation="MRPARLPVPPSRPGRSRVAGQRIRASQRIRRGLAALLALSVAAC
                     TGGLPPRADPSRPTIAYLFDGSPPDAELVSASALAGVELAAHEAGAVEIEPVNLGLDR
                     TEVMSTLGALAEDSAVVAAVIAPWTAPPAGAIDLLAAHGLPVVSLSWAWGPPSDGDGV
                     WLSLAAGRAQEAVLLLSTADVAPEDATLCLAGDEHPTSRALLATAAELARAAGDPDIV
                     MAGTVAEGDVPIADPVADRIEEERCPVLAWTGGTTGAEALLSRLPESPALVVGASRIG
                     TDDGLALATAGRDVITVCACVSVNLSLEPDHMRFVHDLQAASGAPPGPYSVEAYDAGR
                     FLVDLVAGAGSDDPRGILSAALEEPAEIPGLAGSYRFEADGSRAPETLRVGAWRATGS
                     RWLPVEPLTGAPA"
     gene            32387..32470
                     /locus_tag="VF195_10135"
     tRNA            32387..32470
                     /locus_tag="VF195_10135"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32421..32423,aa:Leu,seq:caa)
     gene            complement(32548..33480)
                     /locus_tag="VF195_10140"
     CDS             complement(32548..33480)
                     /locus_tag="VF195_10140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016433733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEX6581216.1"
                     /translation="MGAELRLIGADELHARLPMVAAIDALETAFRTLDPGTGPLRTSL
                     ETPAGSLLVMPAFGEAGVGVKLVTLTSSNPGRGLPYIQAVYVLFDTVTQSPEAVLDGT
                     ALTALRTAAVSGLATRHLAREDAARLVVFGAGVQARAHVDAMCAVRPVTELVVVSRTP
                     GPAGSLVQAGLERGLAARLGEPASVAQAALVCTCTTSREPLFDGSLLTPGVHVNAVGS
                     HRPDARELDTETVRRGRVVVETREVALAEAGELAIPIADGAIEAGHVAADLAEAVRGA
                     EVRRSTEDLTVFKSVGMAFEDLVVARAIVDAPST"
     gene            complement(33484..34224)
                     /locus_tag="VF195_10145"
     CDS             complement(33484..34224)
                     /locus_tag="VF195_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581217.1"
                     /translation="MTAEAAVRGAPILELRNVSTHYGLVAVLRNVDMDIYAGEMVCLL
                     GGNASGKTTTLKTILGYVTPSEGEVVLDGNVVSGMSTTEVVRRGVSMVPENRRLFPDM
                     TVEENLRLGAYLRRDRSSVEAARETVFETFPRVRERLKQKAGTLSGGEQQMVAMGRAL
                     MADPKVLLMDEPSMGLAPILVEQVFEIIKKIRELGKTVFIVEQNANMALSIADRGYVI
                     QTGQVVLADTAENLLQNPLMREAYLGEL"
     gene            complement(34221..35030)
                     /locus_tag="VF195_10150"
     CDS             complement(34221..35030)
                     /locus_tag="VF195_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581218.1"
                     /translation="MSDAASSGSADAVLEVRGLEKHFGGLYALSGLDVEVHRGEIVSV
                     IGPNGAGKSTLFNVITGIYEPDAGDIRYQGSTIVGLRPHQVVKLGIARTFQSVRLFAN
                     MTILENAMVGQHCRSKGGVFGSIVRTPGVRAEEERIRQRAEEALSFFGKRLSGYRLEQ
                     PAFSLSYANRRRLEMARAMATDPQLLLLDEPTAGMNPRETLELRDHIVRMRDELGLTV
                     VVIEHDMRVVKGVSDRVVACDYGQKIAEGTYEEVANDERVIEAYLGTKAAG"
     gene            complement(35027..36811)
                     /locus_tag="VF195_10155"
     CDS             complement(35027..36811)
                     /locus_tag="VF195_10155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6581219.1"
                     /translation="MTAARRWARTGALAGVVTIFLALVGLIGGFTDVFLIGDEITFAG
                     VMLLLPAFLAGFVAAAPRIQGGERREMPLGEAAVAAAVAAASAGVVFALAVLLADLIG
                     DERIRPIFLNVSPSLMDFVAFGQSTVVGAVILVALSTIAGVLGGLLRVAPPVVRRSVT
                     AAVTVTLLFGFLQRVIPIALDQLSLERDWLYHPIHDGLTWIGAAVVAAVSAGVSLLNT
                     RYGGRVRGSLSSGIGGRSGPAMSPVRLIASVAAVALLIAAPLLLGSVISEVLGTVMVF
                     ALLGIGLNIVVGYAGLLDLGYVFFFALGAYSLALLTGATLNAYGIAPEPAISGGLNFY
                     VAIPIVMLIAAGAGVLVGAPVLRLRGDYLALVTLGLGEMVTVLVQSPWLVPLVGGPSG
                     MRGITDAAIGGFAFRDPQRFYYLALAFVALAAFLSWRLATSRIGRAWTAMREDEQTAD
                     AMGISTTRFKLLAFAIGGAIGSLGGALFAVKIGSLTPASFQVIVSIQVLGLVILGGLG
                     SIPGVLVGALVLVGLPGLLREFEEYRFLAYGAAVVAVMVLRPQGLVPNVRRSRELQDE
                     ERAQDEWAKAVEEGETPGELGPPVEVTT"
     gene            complement(36808..37887)
                     /locus_tag="VF195_10160"
     CDS             complement(36808..37887)
                     /locus_tag="VF195_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX6581220.1"
                     /translation="MAVARLGRFERLPWRRIILVVLGIVVGGVVVLGAWRTLGLDRAQ
                     GGYSAGAWRDFIVQGAAQGAIFAMIALGYSLVYGILRMINFAHGEVFMAGAFGSFFFA
                     DAYHKSGFLNRSPLLALAIVTIVAVAISVGTAVLLERLAYRPLRNAPRLVPLITAIGA
                     SLFLQNAFRGFFGPQPYGYPKPTVLAGHVTVLGVPVQRTQLLVLVVALICMIALQWFV
                     ATTRTGRSMRAVAQDREIASLMGINVDRVIVVTFAIGGVLAGIAGVLFSLTFEQVQFT
                     MGFRPGIAAFTAAVLGGIGSIGGAALGGFVIGLLQAVGPPLILTGFGIPSVFSLRDAF
                     VFLVLVFVLVFRPGGLLGTGEAEKV"
     gene            complement(37991..39310)
                     /locus_tag="VF195_10165"
     CDS             complement(37991..39310)
                     /locus_tag="VF195_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6581221.1"
                     /translation="MKHRIWFKALALVAVLTIVATACADDDGGGDGGPGAGEVDCATV
                     EFGCVEVGADEPINLGAAQVISGADASLGQDQVNAINLAIDYLDESFDQTPGTLLDHP
                     VELTVEDELCSAEGGQTAGTALAADTSIVAVVGTSCSSAALGVADTILGDKGITLISG
                     SNTSPALTDPELHNPFYARTAHNDRIQGAIGAEFALGEDLGATTAATIADESPYTQGL
                     VGAFEANFEAGGGTLTGSEQVNSEDTDFKPVLRSLAEQDPDVLYFPIFVAACTLIIRQ
                     AQDIMPDAELIVADGCLSSDTLKNAGPAADGVWASSPDLSAFQTNEFYSGEFIPAYQE
                     AFGTEPLAVFHAHSYDAANVVFAAIEEVAIDNGDGSLSIPRQALRDAIFATDGYEGVT
                     GTISCLDTGDCATDVTIGLFLAPGWPVEGGTDDPAVYTDTKSLDEVL"
     gene            complement(39430..40200)
                     /gene="trpA"
                     /locus_tag="VF195_10170"
     CDS             complement(39430..40200)
                     /gene="trpA"
                     /locus_tag="VF195_10170"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEX6581222.1"
                     /translation="MGRLEDALRARRDAGGQAFVPYVTGGYPGVDAGLLRRLAAAGAD
                     AIEVGIPFSDPVMDGGVIQEASAEALRAGARPGDVLATIREAAIEVPVAVMTYVNPVV
                     RRGISSFLDEAVAAGVTGVIVPDLPVDESSDLGRESAARGVDAVLLAAPGTHEGRMAE
                     IGRRSRGFVYCVATYGVTGARDRVEETAGAVVAALRLHTDLPLLVGVGIGTPAQAAEV
                     CAFADGVVVGSALMHRIVGGDAEGAIQLATAFREAIPS"
     gene            complement(40201..41391)
                     /gene="trpB"
                     /locus_tag="VF195_10175"
     CDS             complement(40201..41391)
                     /gene="trpB"
                     /locus_tag="VF195_10175"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX6581223.1"
                     /translation="MTATTLPDARGRFGTYGGRYVPEVLIPALDELAEAWAELREDAG
                     FRAELSTLSETFVGRPTPITRADRLSTQLGYEIWLKREDLAHTGAHKINNALGQALLA
                     KRLGKERIIAETGAGMHGVATATACALLGLPCVVYMGEEDTRRQAPNVSRMKLLGAEV
                     VPVTAGSRTLKEAVNEALRDWAASVRTTHYIIGSVVGPHPFPVLVRDLQRVIGDEARP
                     QFSDATGGADPDVVAACVGGGSNAIGMFTAFVGAPGIRLIGVEAGGRGPGLGEHCSSL
                     TQGHPGILHGAMSYLLQDDDGQVAQTHSISAGLDYPGVGPEHAYLRDAGLAEYVSASD
                     GDALDGFQALSRSEGIIPALEPAHVIGWLLHRPLEQGTSVLVCLSGRGDKDLDTVRAS
                     LGIS"
     gene            complement(41388..42173)
                     /locus_tag="VF195_10180"
     CDS             complement(41388..42173)
                     /locus_tag="VF195_10180"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005247490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEX6581224.1"
                     /translation="MGFLTDLVDRLRQDLREHPLDDAMLMARAAAMPPTRDFEGALRG
                     STPPALIAEVKRASPSAGAIAKDANASVLARGYEAGGAAAVSVLTEPRHFQGSLADLQ
                     AVRSSVSIPVLRKDFVVHPAQVIESRASGADAVLLIVAVLTDAELRALLEAAADLGLG
                     TLVETHSDEELRRVLDTDAPVIGVNARDLETLDVDVEGALARLGGLPADRLGVLESGV
                     STRGHVDAALAAGASAILVGETLMRAEDPARAVRKLLWRELRT"
     gene            complement(42200..42910)
                     /locus_tag="VF195_10185"
     CDS             complement(42200..42910)
                     /locus_tag="VF195_10185"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEX6581225.1"
                     /translation="MFEVIPAIDVKDGALAHLTPTGLMRIDAFAGDPVAAAAAMVEAG
                     ATRLHVVDMDLAFDGEPRNLDVVSAIVSLRVRVQAGGGVRTAEEVDALLSVGVERVVL
                     GSAALADEVLARDLLGVHGPRLIVGIEVGDGDTIRSRGRDPVDLPLMDTLGWLSAARA
                     QGFLVTAVARVGSSIGPDVGAVKRVVRAGRPVIAAGGIHTVDDLRALRAAGALGAVVG
                     RAALEGTLDLAEARSALA"
     gene            complement(42975..43937)
                     /locus_tag="VF195_10190"
     CDS             complement(42975..43937)
                     /locus_tag="VF195_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6581226.1"
                     /translation="MRNVGDAAVQQVMDARAAKGRFGSFSDFCRKVDPSVLTKRALES
                     MMLAGAFDSLGYQRRALLIGHDKVSGPIVAERKAEAAGQFSLFGGDSDVGQIDESVLE
                     GEEFDKPELLRFEKEMLGSFVSDHPLLEVADDLRAQSTHEIGDLEGADDGELVTVAGI
                     IGGVSRKYTKRGEPYAQFRLEGLSAGVDVVAFPSVYEADPDLIASDRIVLVVGRIDRR
                     GRELQIRANDVREPILTGSAPSAGGESVVIDLTAAACTDAVLSKMAELLRSRPGSAPV
                     RVRFHSIDGVRPLDMGEFLVDPSGLMGELAGLLGSGAARVERVT"
BASE COUNT         6713 a        15513 c        15050 g         6815 t
ORIGIN      
        1 tcgtccgcca cgcggccgcc gtcgagaggg acgttgaagt cgacgcggac gaagacccgg
       61 gagccggcga cgtcgccgag gtcgtcgagc gtccggagcg ccactagagc ttcgaggcga
      121 ccaggttcac gaggtcgacc aggcggttcg agtaccccca ctcgttgtcg taccagccca
      181 tcaccttgac catgttcccc atcgccatcg tcgagaggga gtcgaaggtg cacgaggccg
      241 gcgaccccac gatgtcgctc gagacgatcg ggtcttcggt gtagacgagc ttgcccttca
      301 gccggtcggt ctcggaggcg gtccgatacg cgtcgttgac ctcttcgacg gtgacgtcgc
      361 ggttgacgat cgccacgaga tcggtgatcg atccgtcggg cacgggcacg cgcatcgcga
      421 tgccgtccat cttccccttc agctgtggga gcgcgagcga ggtcgccttc gccgccccgg
      481 tggacgccgg gacgatgttg atcgcggcgg cgcgcgcccg tcgcagatcc ttgtggggaa
      541 ggtcgagcag gttctggtcg ttcgtgtacg cgtgcaccgt ggtcatcagg gcgcggtcga
      601 tcccgaagga gtcctcgagc accttcgcga gcggcacgac gcagttggtc gtgcaggaag
      661 cgttcgagat cacgtgatgc gtggtcgggt cgtagacatc gtcgttcacg cccatcacga
      721 tcgtgatgtc ctcacccttg gcgggggcgc tgatgacgac cttctgggcg ccggcttcca
      781 ggtgcgcggc ggccttctcc cggtccgtga acagaccggt cgactcgatc acgatctcgg
      841 cgcccacctc cttccacggg agctgtgcgg ggtcccgttc cgcgaacacg cgaagctcgt
      901 cgccatcgac ggagagaccc gcgtccgaga cggcgacctc tgcgtccagg cgacccagga
      961 ccgagtcgta cttcagcaga tgcgccaggg tcttcgcgtc cgtgatgtcg ttgacgccga
     1021 cgaagtcgac gtccgcgccc gtgagcctga cggcgcggag gaagttgcgg ccgatccgtc
     1081 cgaaaccgtt gatgccaacc ttcacgctca tcgtggagaa cctcctgggg gcctcgttgg
     1141 cgcctcggtc cctccatcct acgagcggcc tcgcgagctg tccggccgtg tagcgtggcg
     1201 ccgatgggcg acggcgtgga cgcacggaac cgggcgtccg ccgaccgctt ggcggcgctc
     1261 ggcgcgaggc tctccgacga cgagctgagg accgagatcg acccgccctg gaccgctgcc
     1321 ggactcttcg cgcacatcgc cttctgggac cgcttcgtgc tggagcgctg gaggctggcg
     1381 gccgagcgtg gcgagcgaac gccgatggcc gtcgacggcg gtctgatgga tcggatcaac
     1441 gacgcctcgt tgcgccagtg gatgtcgatc ccctctcggt gaccgtcgag gagtgcctga
     1501 gctccgcggc cgctctcgat gcgttcgtcg aggacgtcgc ggatgacgtc cggggggagc
     1561 tcgccgccga gggtcgggaa cggctcgtcg atcgctcgct ccatcgcgcc gaccacctgg
     1621 gtaccctcga ggcggccttc cccggaggat gaacctcgtc agtcgcggtc gacgtcgcgg
     1681 tggctcacgg agacgcgatg cccgtgctcg cggaaccagt cggcgaggtc gtccgcgacc
     1741 acgacggagc ggtgccggcc cccggtgcag ccgatcgcga tcgtcagata cgacttccct
     1801 tcgtccacgt agcccgggac gacgacgtcc aggaggccct cgagccgcgt catgaaggac
     1861 ccgtactcct cctgcccctc gacgtactga cgcaccttct cgttgctgcc cgggagaggg
     1921 cggagctcct cgacccagtg cgggttgggg aggaatcgca cgtccatcac gaggtcggcg
     1981 tcgcgcgggc tcccgtactt gaagccgaag gacacgatgg agacgagcag gccggcctcg
     2041 ggaggtgtgt cggcgaacgt ctctcgcatc cggtcgcgga ggtcgtgcgg cgtcagtcct
     2101 gtggtgtcga tcacgaggtc cgcgtcggcc cgcaggctct ccatcatcaa gcgctccttg
     2161 cggatcccct cccccacccg atcacccgga gccagcgggt gacgccggcg ggtcgcctcg
     2221 taccggttga cgaggtcctc gtccgaggcg tcgaggaaga tgatcctgga tgggacccgt
     2281 tgctcccgca gctcctcgag tgccttcgac agctcgccga agaagacgcc gccccgcgcg
     2341 tccacgacga tcgccacccg cccgggaccg cccgggctcg cggccagctc ggccatcttc
     2401 ggcaggagcg ccggcggcag gttgtcgacg acgaagtatc caaggtcctc gaggctccgc
     2461 gcggcctcgg agcgcccggc cccggacagc ccggtgatca acaggaaccc gggggcggcg
     2521 gtctcccgcg ccgatcgctt cacggcccgt gccatcacgc gctctcccga cgatccgtga
     2581 ccgataccgg atgcagccgg cgatggatca cgtccgcgag ccggggcccg acccccggtg
     2641 tggcggcgag ctcgaccgct cccgcttgac ggatcttcgt caaggagccg aagcggttca
     2701 tcagcgcgcg cttccgggac ggccccaccc cggggatctc gtcgagcggc gaggagaggg
     2761 cccgacggtc gcgccgttgg cggtgatacg tgatcgcgaa gcggtgggcc tcgtcacgga
     2821 tgtgttggag cacgaagagc gcctccgatc cgcgcgcgat cgacagcgga tccgggcggt
     2881 cggggaagta gatctcctcg agtcgcttcg cgagcccgat gtgcgggatg tgcaagccga
     2941 ggtcctccag gaccctcgtc gcgacgctca gctggccgcg accgccgtcc accaccacga
     3001 gcgccggtgg gtacgagaag cgtctccgac cccccggcgg ctcggccttc tccttcagca
     3061 gccgcgagaa ccgccgggtg agcatctcct ccatgctcgc gagatcgtct tgcccgggca
     3121 cgcccttgat ctcgaaccgg cggtagtcgg cccgcttcgg gagaccgtcc tcgaagacca
     3181 ccatggatcc caccgtgtcg ctcggcccga ggttggagat gtcgtagcac tcgatgcgga
     3241 ggggcgcctg ctcgagcccg agctgatcgg cgagctcgga cagcgcccgg gaccgcgccc
     3301 cgaaatccga agcgcgccgg agcttgtgcc gatggaacgc ttcgcccgcg ttgctcccca
     3361 cgacctccat caggcgccgc ttggcgccgc gcgcaggcac ggcgatcgtg acgtgggaac
     3421 cccgacgggc ggagagccag gcttccagca cctctcgctc cgccggctca gttggcacca
     3481 ggatccgcgg cggcacgtct tcgcgctcca tgtacagctg ccggaggaac gacgcgacga
     3541 gctcgggccg gtcgaggtcc tccacgcgat cgacgatcca tcctttcctg ccgaggaccc
     3601 gtccgccgcg aaccgtgaag acctggaacg ccgcctcgag gtcgtcctcg gcgaggccca
     3661 cgacgtccag gctctcgggc tggacgagga ccatctcctg gctctccagc gcccggcgcg
     3721 ccgcctgcag ctggtcgcgg aaccgcgccg cctgctcgta ctcctcccga cccgacgcct
     3781 cggccatctg ctcttcgagg cgccggagca cgggcttcga gttccctgcg aggaagtccc
     3841 cgagcgcgtc gacgtcgctg cggtacgcct cctccgtgat cccggtggcg gccgggacgc
     3901 aggggccgga gcaccggccg atgtcgtagt acaggcaggg ccgcccggcg cgggcgcgct
     3961 ggtcgaagaa gtggttcgtg caggtgcgga tggggaacac tcgcgtcagg gcgtccaggg
     4021 tgtcccggat cgcccaggcg tgtccgtacg gcccgaagta ccggaccttc ttccgcttcg
     4081 tcccgcggag gacctgggct cgcggccacc tctcccccac cgtgagcgcg aggtagggat
     4141 acgacttgtc gtcgcgatag cggatgttga accgcggcag gtactgctgg atcaggttga
     4201 actccagcat caacgcgtcg acctcgcccg acgcgacgat ccactcgacg ctcgccgccg
     4261 cgtccaccat ggcttcggtc cggggatgga gcggtcggga ccagtagttc gccagccgct
     4321 tcctgagcga gcccgccttc ccgacgtaga cgaccctgcc gtcctcgtcc cggaagaggt
     4381 aagcgccggg agcgtccggg atggtggacg atgccggctt ggtgaagctc acccacccat
     4441 cgtagcgccg ccgggtgacc cggctgggaa cgtggtgcgg ctaaccgccc ccgcccccgc
     4501 cgcccccgtt cccgcctcca cccgcgacca ccgccaaggt gccgaacatc acatcggggt
     4561 gagcctcaca gaggaagtag aggtcgcctt cctcctgggg gtcgaccggc agctcgtcgg
     4621 tgtaggtgcc gggcttcagg tccgttgcag tgatgttctc cccgtccgcg tccggacgga
     4681 tggcgaggtt gtgcgtcacg ccctcgtctt cgttattgaa ggtgatcgtg aatccctcct
     4741 ccgccggcgc cacgagacag gtgggctcga aagcggtcgt gggagctgtc acctccaaga
     4801 ccggttcccc atcgccgccg caatcctcgg gtggtcccgc aggggccggg ctcggcagcg
     4861 gtgtcggggc ggcgatcagc aggtctgaga tctcgccgaa cgtgccgagc cggaggtcgc
     4921 cctcgttcgc gtcgtaccag gcgagataga ccgatgagcc gtcctcggtg atggcgaggc
     4981 tcgggttcac tccgctgctc gtgtcgggga gctcgacctc ctcgataggg ccgtcagcct
     5041 gtgaggccag atggatgcca tcgccgttct gccacgcgac ccagacggtc ccttgttggt
     5101 cgaccgcgac tccggtgctg ggtggcgtcg gcacgtcggc ggaccggtcc cctccgcccg
     5161 tcgcgagcgc ggcgacctcc tcggccgacc acgacccggg acggccggtg gcgagcgcca
     5221 ccccggtctc cgtgtagtag gcgatcgcgg ccgtatcgcc cgccgaggag accgacgcac
     5281 cgcccgcggc gtcggtggcc accacctcgg tcgtccaggc ccctccctgc cgccgtgccg
     5341 cgatcacctc gccggaaagc ggatcggcgg tcaccacgag cggttcgccg ccgaggaggg
     5401 cgagctgcgt tgccggcggg catccctcgc cgcaccggga gagggtggcc accgtggtga
     5461 ccctccagtc ctcgccgaca cgctcggcca cccgaacctc cggctgggcc ccggcgatcg
     5521 tgtaggcgac gatcggtgcg cccgtgccgt cgaccacgac ggagggtgcg ctggcctcgg
     5581 gggtctcctc cacggcaccg atctcgaacg gcgtggggcc gatcccgtac cagacgcctc
     5641 ggtcggtggt ccagacggcg tggacgtcgc tgcccgcgat cgcgacatcg gtccccgctg
     5701 cccgcggctt cgtcagactc gagagggaag gttcggcggc gggctcgaac ggcacgggga
     5761 ccccgctcgg cgtgtccctc ggctgggcga tcgcgcctcg gttccagatc tggtcggggt
     5821 tgaagctcac catcagcacc gcgccggcgt cgtcaccgtc ctcggtcgtg aggaacggtg
     5881 agcccacggg tcgcgcgatc gggatctccc cctcttccag ctgcgccggg aacccgtagt
     5941 aggagatggt ggggagtcct tgcgagtcga cggccaccga cggcgtaagc ccgacgtcgt
     6001 cgatcgagtc ggggaccatc ggtatgaact gccgtccctg cggggcctcc acctccgacg
     6061 gcgggatgct tccgcatgcc gcggcgagga cgaccagggc cagggccagg gagcgcggag
     6121 acggtctcga cacgacgggc cgcatcctag caagcatcca cagccgcact gtgacgctca
     6181 cgcagaggcg ccgaccccga gcggcaggac ccccaggacc tcctcgagga accggccggt
     6241 atgggagtcc gcgttggccg cgatggcctc cgggggacct tcagccacga tgcggccgcc
     6301 ctggtccccg ccttcgggac cgaggtcgat cagccagtcc gcggtcttca cgacgtcgag
     6361 gttgtgctcg atcacgagca ccgtgttccc ggtggcgacc aatcgctgga ggacctgcag
     6421 cagcttcctt atgtcctcga agtggaggcc ggtggtgggc tcgtcgagga tgtagacggt
     6481 gttgccggtg gcgcgcttgt ggagctcgct cgacagcttg atccgctgtg cctccccccc
     6541 ggagagcgtg ggggcgggct gcccgagctt gatgtagccc agtccgacgt cggacagcgt
     6601 ctgcatgtgc cgcttgatcg gcgggatgtt ctggaagaac tcgagcgctt cgtcgacgct
     6661 catctcgagg acgtcggaaa ccgtgcgacc cttgtacttc acctcgagcg tctcgcggtt
     6721 gtaccgccgg cccttgcaga cctcgcacgt cacgtagacg tcggggagga agtgcatctc
     6781 gatcttgatc tggccgtcgc cggcgcagtt ctcgcaccgc cccccgcgga cgttgaacga
     6841 gaagcggccg ggctggtagc cgcggagtcg ggcctccggg acctgggcga agagcttgcg
     6901 gacgtgatcc cagacccccg tgtaggtggc cgggttggac cgcggcgtgc gaccgatcgg
     6961 cgactggtcg atgtcgatca ccttgtcgac gtcttcccac ccgaccatcc gtcggtgctt
     7021 gccgggcaac acccgcgagc gatacacctt ctgcatcagc gcgcggagca ggacgtcctg
     7081 gacgagggtg gacttgccgc tcccgctcac gccggtcacg cacaccatca caccgagcgg
     7141 gatcttcacc gtgatgtcct tcagattgtg ctgccggaca ccctcgaggc ggagccaccg
     7201 cttcggcgcg cgccgtgacg acggcagggg gatcgatcgt ctcccggaga gatacgcacc
     7261 ggtaagcgac tcatcggatc cgagcagtcc cttcaggtcg cccgagtaca cgatccgtcc
     7321 accgtgctcc cccgccagcg ggccgatgtc cacgatgtgg tcggcttcga tgatggtggc
     7381 ctcgtcgtgc tcgacgacga tcagcgtgtt cccgaggtct cggaggcgga tcagggtgtc
     7441 gagcagccgg cgattgtccc gctggtgcag accgatcgat ggctcgtcga ggatgtagag
     7501 cacgccgacc agccctgagc cgatctgggt cgcgagccgg atcctctgcg cctcccctcc
     7561 cgccagcgtg cccgccggcc gggagagcgt caggtagtcc aggccgacgt cgacgaggaa
     7621 gccgagccgg gcgcggatct ccttcagcag ccgctcggcg atcatctcct cgcgctcaga
     7681 gagctccatc gactcgagga aggacagcgt cttcctgatc gacaggtcgg tgagcgcgga
     7741 gatgtggagc ccgtcgaccg tgaccgcgag cgtctccgga cggagccgcg caccggtgca
     7801 cgaccggcag ggtgtttcgc gcatgaactg ctcgagcttc tcacgcgagg cgtccgagtc
     7861 cgtctcctcg tgtcgccgtt cgatgttcgg gagcacgccc tcgtagctgg tgtagtacga
     7921 gcgctgtcgc ccgtagcggt tcttgtagcg gacgtagatc tcctcgtcgg atccgaacag
     7981 cacgacgtct cgcgcccgct tgggaagctt cttccatggt gtgtcgagcg agaagccgtg
     8041 ggcctcgccg accgcctcga ggacccggta ccagtactcg agggtgttgc tcgcccacgg
     8101 cgccaacgcc ccttcgttga tggagagggc ggggtcggga accacgagat cggcgtccac
     8161 ctcgagacgg gtgccgagcc cgtcacaggt ggaacacgct ccgtacggcg agttgaacga
     8221 gaagttgcgg ggtgccagct cgtcgaacga cagcccgcag aacgtgcatg cgagcttctg
     8281 cgagaacgtc tggagctcct cgtggccttc gtgggtggtc accgcgatcg acgcgatccc
     8341 ctccgccagc tcgagggcgg tctcgatgct gtccgcgaca cgccgccgga tcccctgctt
     8401 cgcgacgaga cggtcgacga cgacctcgat cgtgtgcttg aagttcttgg ggagccggat
     8461 cgactccgac agctcccgta cctcgccgtc gatccgcgcg cgagggaagc ccttgcgcgc
     8521 gagctcctgg agcagcttct cgtactcgcc cttgcgaccg cggatcacgg gcgcgaggac
     8581 ctggaagcgg gtgccctcgg gcagctggag aacctgatcc acgatctggt cgggcgtctg
     8641 tcggccgatc ggacggccgc agttgtagca atggggatgt ccgatgcgcg cgaacagcac
     8701 acggaggtag tcatagacct ccgtgatcgt gccgacggtg gaccgggggt tgcgtgacgt
     8761 ggacttctgg tcgatcgaga tcgcgggcga gagcccctcg atgaagtcga cgtccggctt
     8821 ctccatctgg ccgaggaact gacgggcgta cgacgagagg gactccacgt agcggcgctg
     8881 gccctctgcg tagatcgtgt cgaaagccag ggaggatttc ccggagccgg acagccccgt
     8941 gaacacgatc agctggtcac gcggcaggtc gagcgtgacg ttcttgaggt tgtgctcacg
     9001 ggcgccgtgg atgacgagtc tctcggagcc cacgggtcga gtgtaccggc ggctcgcgac
     9061 acgctctgtc cgttggcgcc gctcccggtc atggcgccgc taccggcccc tcccgggatc
     9121 ggcggcaccc gacgacctgc atcctccccg cctatccttc gcccatggac agagacctgc
     9181 gttcgtcgcc gctgtaccag gagatcgagg agcacttccg gaaggtctac gagccggggt
     9241 tcgggaaggt ctccgaggcg tccgaccctc ggccctcccc cgacgggcgg tgggtggcgt
     9301 tcacggggtc catctgggag gagctcgagg ggacgccgaa gacccgggtc tgcctcgcgc
     9361 tggcaggtgc ggaggatggg agcgccagac aggtcaccgc cgggcccgag cgtgacgccg
     9421 gggcacgctg gtcgcccgac ggccggcggc tctccttcct ctccgaccgg cgaggtaagg
     9481 ggcggatgca gctctacgcg ctcgacgcgg atgcgctcgg ggaagccgag gccctgcccg
     9541 aggtggaggg gacgatcgag gagcacgcct ggtctccgga cggctcccgg atcctcgtgc
     9601 ggacggccgg gatgcacgcc gacggggccg gagcggacgg ctcgggggcg ctcgcgggtg
     9661 agaaggacct cccggactgg gtccccgccg tggactcctg ggaggaccgg caggtctggc
     9721 gacggctctg ggtgatcgag gcgtcctccg gcgaggtccg agccctctcc cgggagggcc
     9781 tgaacgtctg ggaggccgag tggtgcggtg acggagccgt ggtcgctgtg gcgtcggagg
     9841 atcccggtga gtccgcctgg tacgccgcgc cggtcgtgct gatcgatacc gagacaggcg
     9901 aggatcgcgt cctcgccacg agcgacgtcc agttcgggct cccggtcggc tctcccgacg
     9961 gctcgcgcgt ggcagcggtc gaggcgccct gcagtgaccg gcagctcgtg tccggtcgcg
    10021 ccgtggtggt cgacgtgcag agcggcgagc gtcgggtcgt cgagatggag gacgccgata
    10081 tcacgtggct gggatggcgc gcggacggcg ttctgtcgta tgccgcgctc cggcgagggg
    10141 acacggtgtt cggggacatc gaccccgcat ccggcaaccg gaccgagacg tggtcgacga
    10201 gcgaggccgt gggcgcctgg gctccctacg cggcaccgct cggcgccgac gggttcgcct
    10261 tcatccggca gggcttcgat cggcccatcg aggtggcggt cgtggagggc ggggtggacc
    10321 ggacggtcgc ttctttccga cacgaagggc acgatcacgc ccgcggctcg gtcggccgag
    10381 cggaacgggt cacgtggaac gcccccgacg gcctcgagat cgacggcttc ttgctgacgc
    10441 cgccggggga tggacctcat ccgttgatcc ttcacgtgca cggcgggccg gtgtcgtcgt
    10501 acatggagtc gttcccgcgc ccatccttcg cgtggttggt ctcccgcggc tacgcgatcc
    10561 tcttgccgaa cccccggggg tcgacgggac gcggtcgcgc gttcctcgaa gcggtgatcg
    10621 gcgacatggg gggcgcggac gcccgcgacg atctcgcggg cgtcgatgcg ctcgtcgagc
    10681 gcggtgtcgc cgatccggga cggatcggcg tcaccggtgg gagctacggg ggcttcatgt
    10741 cgtgctggct cccggtggtc gaccagcggt tcaaggcgtc ggtcgcggtc tcccccgtga
    10801 ccgacttcta cagccagcac tggaacagca acatcggcgc gtgggacgcg tggttcctcg
    10861 gtggtgagcc gcaggacgat gcgccgcagt accgggagcg cagcccggtc ttcttcgcgg
    10921 accgcgtaac gacccccacg ctcctgaccg ccgggacgga ggatcgctgc acgcccccgg
    10981 ggcaggcgat cgagttctcc cgcgcgctgc gggcgcgagg ggtccccgtc gaggtggcga
    11041 tctatcccgg cgaagggcac ggagtgcgga agctccccgc gtacctcgat ctcgtggctc
    11101 gcacgacggc ctggttcgaa cggttcatgc cggcgagggg ctagtggacc tcaccgcctg
    11161 gcgggacgag ttccccgtgc tggagcggaa gacgtacctg atcagcgcgt ccctgggtcc
    11221 cgtctcccgg ctatcgaggg ccttcctcga ggagtacctg gacgtgtggg gctccaaagg
    11281 tgcgcccgat ctcgtctggt tcgaggacat cttcccgcgg atggcggcgc tcaagcgttc
    11341 gttctcggcg ctcgcgggct gcgaccccga tgaggtggcg ctcaccacga acatctcgat
    11401 cgcgttgtcg acgatcgcgt cgtgcctcga cctgtccggc gaacgccgca ccgtggtcct
    11461 gtccgagctc gacttcccga ccgacggcca ggtctggatc gcgtgggctc gcaaggcggg
    11521 ggccgagatc cgatggctgc gcagtcccga cggcctgacg atcccgctcg aagagtacga
    11581 ccgggcgatc gacgaacgga ccgcgctcgt catggtgaat cgggtcctct atcggtccag
    11641 cgcgatcgtg gacgcgaagg ccctgtgcac gatcgcccgc gatcggggcg cgctctcgtt
    11701 cgtcgacgac taccacgggc tcgggatcgt cccactcgac ctgcacgacc tcggctgcga
    11761 cctctacacg gccggcgtcc tcaagtggat gctcggcggg ccggggctcg tgttcctcta
    11821 cgctcgacgt gacttgctcc cctcgctcga gcccgcgatc acgggttggt tcggccaacg
    11881 cgagccgttc gccttcgatg cggagcgtct cgactatcac cccacggccc gccgcctgga
    11941 gcacggcacg ccgccggccc ccgtgttctt catcgcgcag ggcgggatcg acatcatctc
    12001 gaaggtggga ccggccgcga tccgcgcgcg ccagggcgaa ctcaccgacc acgtcatcgc
    12061 gcgcgcggac ggggccggcc tcgaggtgcg cacgccgcgc gaccgggagg cacggggagg
    12121 cgtcgtgaac gtgaaggtcg gacccgaagc ggagaagatc tgccacgagc tcctggcgcg
    12181 cgacgtctgc acggacttcc gcggcgacgg cctgcggatc agtccgcact tcttcaacac
    12241 cggagaggac atcgaccgat gcttcgacga gctgcggcag ctcgtctgaa ccgtcagcga
    12301 acgacgacgg ggacccgctg gggcatgttg ttcatgaagc ccccgtagtt ccagggcggg
    12361 tccatcggct ggacgttgcc ggcctcgtcg gtcgcacgga cgaggagctc gcgctccccc
    12421 ggccgggcat cccagtcgaa ggaccaggac cgccacgagg aggacgactc gggctccggt
    12481 cccaactccg cgtccgacca cgtcgccccg ccatcgatgc tcacctcgac gcggtcgatg
    12541 tgtccccatc cggaccaggc gcggccgatc agctcatgac ctcccgcgtc gacgaaccgc
    12601 tgcctcgcgg ggaaatcgct gaagccgggt ggcagcatca aggctcggac ggccttcctc
    12661 gtcacacgct cgcccgggcc ctccggctcc tggcggcgct gataggcgga gagctggaag
    12721 ccgtcgaacg ggtcggcgac ggcggtgatc cggcggagcc acttcacgct gcacatcccg
    12781 taccaatccg gcaccagcag ccggatcgga tacccgtgct gcggtgggag cggctggccg
    12841 ttcagctcgt aggccaacag cacatcgttc ttgacgatgt cggccaacga gaagccccac
    12901 tggtagtcct gctcgacgtc tccctggatc ccgtggtcgg ctccggtgaa cacgagctcg
    12961 agggcctcgg ggcgcagccc ggcgtcctcg aggatcgggg gcagcggcgt gcccatccac
    13021 tcggcgttgc cgacggcctc gaggagccac ggctggctct cggcccgcgg aggcatgagg
    13081 gctcggccgt tcccggcgca ctccatcgtc acgggacgcg tgaccgccgg ccgtgagcgg
    13141 atgtcatcca tcgacagcgt caagggacga tcgacgaggc catcgacgtc cacggtccat
    13201 gccgtctcgt ccgccgcggg gatgtcccag tggatcacga ggtagtgcat cccgatcggc
    13261 gtgacgtcgt accgcagccc ctcgagctgc atcccgtggt tccgtgcagc ttggccgagc
    13321 tccttctgtg tcacgccctg ccacgtcgtc gtgtcggtct ggaccatccc gatcacctcc
    13381 cgagcgtcat cgtgccatga agcggccggc gggtcagcct ccggcgacga cccgcccggc
    13441 gcggatcacc agcaccacgt cgtcgagcgc tcggagatcc tcgagcgggc tctggcggag
    13501 agcgatcagg tccgcggggg cgtcgggtgc gagcggcgcc cggatcatcg cctcgaggac
    13561 ctcctccggc tccaagcccg cctccgccat gagcagcgcc tcccgcgtgt ggatccccgg
    13621 tgggatcgag ccgttcccga ggtccgtccc gtagacgatc gtcccccccg cgacgtggaa
    13681 gcggcggagg ttgtcgagcg ccgtgcggat ctcgggcgtg tcgcggccga agctcgagat
    13741 gtcgagggta gagacgatcc gcatctgcgc cgccgcggat ccgatcacct ggtccgacaa
    13801 ccgcgtccac ggcgtgtggg ccagctcgtc cacccccgcc ccgagcgcgc gctcgacctg
    13861 ccccttgccc tgcgcgtgcg cggtgaccgg gaggtcggcg gcgtgggcgg cgtcgcagat
    13921 cgacgacagc tctccatcgg tgggcgtggg accggcgtcc gtgttcagcg ccaccttgat
    13981 cgcggcggcg ccgagccgcg cgagctcctc cacggctgca gtggcatcgt ccgtgccctt
    14041 gatctcgcgg cccgtcccgg gcggcgccca gggatcctgg gtgggatatc cgccgggacc
    14101 cgtgagcatc ggcccggcag ccttgatcaa cggcccctcg aaggacggca tctcggacgc
    14161 gtcggcgagc gagaagatcc gctcgaccgg ccacgcgagg tcgcggaccg cggtgacgcc
    14221 gccggcgagc accgccgccg ggtccgacag ctcgatgtgg acgtgacgat ccgccacggc
    14281 cggcatcagg aagccgtcga cgacgatctc ccgatcggac gcctcgaccc ccgtggccgc
    14341 gcccgcatac gtgatccggc ctccctcgca ggcgatctgg gcgtcttcga ggaacccgcc
    14401 cggtccgagc cacccggccg gggcgcggac ggagaggcgg ttcacgctcc gaccccggca
    14461 gcgcggaggg ccttgagctc cttgcggagg tcgaccacct cgtcgcgcag ccgagcggcg
    14521 tactcgaagc ggaggtcctt cgccgcttgg tgcatctcct cttcgagctc gagcaggagg
    14581 cgctcgagct cctcctgcgg catctccggc cgggccaggc gctgtcgccg ctccttccgc
    14641 ggcgaggtgg cgcgcttggc cgggtcttcg gagatcgaga gcaggatgtc gtgcacgcgc
    14701 ctccggatcg tcgtgggatc gatgccgtgc tccgtgttgt aggccgcctg gatcatccgt
    14761 cggcgctggg tctcgctgat cgcggtcttc atcgagtcgg tgacggcgtc cgcgtacatc
    14821 aggaccttgc cgtcgacgtt gcgcgcggcc cgtccgatcg tctgtatcag cgaggtgtcg
    14881 gagcgcagga acccctcctt gtccgcatcg aggatcgcca ccaaggacac ctcgggcaga
    14941 tcgagaccct cgcggagaag gttgatcccc acgaggacgt cgaactcccc gagccggagg
    15001 tcgcggacga tctcgatccg ctggatcgtg tcgacctccg aatggaggta gcgcacgcgg
    15061 atgcccatct cgatcaggta gtccgtgagg tcttccgcca tcttcttggt gagggtcgtc
    15121 accagcaccc gctgctccgc ctcggcacgg cggcggatct cctcgatcag atcgtcgacc
    15181 tggcccttgg tcggccggat ctcgacctcc gggtcgatca agcccgtggg ccggacgatc
    15241 tgctccacga tccggtcgga caccttcagc tcccaggggc ccggcgtggc ggacatgtac
    15301 acgatctggt ggacccgctc ctggaactcc tcgaaacgga gcggtcggtt gtcgatcgcg
    15361 ctcggcagcc ggaacccgaa gtcgacgagc gtggacttcc gtgagatgtc gccctcgtac
    15421 atgccgtgga gctgcggcac cgtcacgtgc gactcgtcga tcacgaccag gtagtcgtcc
    15481 gggaagtagt cgaggagggt gtacggcgcg ctgccggcgg cacggccgtc gaggtgccgc
    15541 gagtagttct cgatccccga gcagaaccca acctcgcgga gcatctcgag gtcgtacgtg
    15601 gtgcgcatcc ggagccgttc cgcctcgagg agtttgctct gcgactcgag ctccgccagc
    15661 cgcgcctgta actcttcctc gatcgtcacg atggcctgct gcatccgatc ctcgctcgat
    15721 acgtagtggg aggcggggaa cgtggtcagg tcgacgatcg cgtccgtgag gagctcgccc
    15781 gtgagcgggt cgaaccggct gatcttctcg atggtctcgc cccaccactc gatccggtac
    15841 ccgacctgtt cgtagctggg gaacacctcg agggtgtcgc cctggacccg gaacgtcccg
    15901 cgcgcgcgcg tggcgttgtt ccgcttgtac tggatgtcca cgagccgctg catcgcgaac
    15961 tccatcggga ggtccgtgcc gcggacggtc cgcaggatct gcccctcgta ctgctcggga
    16021 gagccgaggc cgtagatgca cgagacgctc gcgacgatca ggacgtcgcg ccgggacagc
    16081 agcgcggccg tcgccgagtg ccggaggcgt tcgatctcgt cgttgatcga cgagtccttc
    16141 tcgatgtagg tgtcggtctg cgggacgtag gcctcgggct ggtagtagtc gtagtagctc
    16201 acgaagtact cgaccgcgtt ctccgggaag acctcgcgga actcgttcgc cagctgtgcc
    16261 gcgagcgact tgttgggggc gatcacgagc gtgggtcgct ggacccgttc gaccaggtgg
    16321 gcgacggtga acgtcttgcc cgtgcccgtg gcccccagca gggtgacgtg ttcctgaccc
    16381 gcctgcacgc cgtcgacgag ctgttcgatc gcctccggtt ggtcgccgga cgggcggaac
    16441 tcggacgtga tcttgaacgg cggcatgggc cgccagtgta ggggggctcg gagacaagcc
    16501 cggatctaca tccggcccgc ggcgatgatc cggtccagga accgccgggt ggcctcctcg
    16561 cgcggctcgg agaacatccg ttcgggcggc ccctcctcca ggatccggcc ctcctcgagg
    16621 aacgcgaccc gattggcgac gtcacgggcg aaccccatct cgtgcgtcgc gatcaccatg
    16681 gtcatcccgg ccgcggcgag ctccctgatc gtgtccaaca cctcggcgac gaggacggga
    16741 tcgagggcgc tggtgacctc gtccaggagc atcacgtcgg ggtcaagcgc cagggctctg
    16801 gcgatcgcga cccgctgctg ctgaccgccc gacagacggt cggggtagtc atccgccttg
    16861 tcggccaagc cgaaccgctc caacagctcc accgccttag gcctcgcctc tgcctgcgac
    16921 cgaccaaggg ccttctcctg agccagcatc acgttgcgga tcaccgacat gtgcgggaag
    16981 aggttgaagg cttggaacac gatccccacc cgacgccgga ccgcgttctc gtccacacgc
    17041 gaggcggtga tctcgactcc ctcgatccag atgcgcccgg cgtcgacccg ttccaggagg
    17101 tcgatgcaac ggagcagtgt cgacttgccg gaaccactcg ccccgatcag ccccacgacc
    17161 tcgtggtcct cgaccacgag atcgatgccc cgaagcactt cgagctcccc gaacgccttg
    17221 cgcacgccct ggatgcgaac ggcttcggtc acgtcgtcac ccccgcctgc cgtcgacggc
    17281 gatcccgttc gatcaaccag tcggtcaggc gggccagcgg gatcgtcatg aggacgaata
    17341 ggaacgccgc gaccacgaag ctcgagtagt tgaagcggta cgcggagtag atctgcgccg
    17401 ctcgcgccgc ttcgacgaca ccgagcaccg ccagcagagc cgtgtccttc tggaggctca
    17461 cgaaatcgtt caggagcgga gggatgaccc tgcggatcgc ctgcgggagc acgacgaagc
    17521 ggagcgattg caggcgggac aggccgagcg atctcgccgc cgcaatctga ctggggtgca
    17581 cggattcgat ccccgctcgg tacacctccg ccacgtacgc cgaataggac aggaccaggg
    17641 ccccggtcgc gtacaccacc tccgattgcg tcgagagacc gcggatgccg agggctggaa
    17701 ggccgaaccc gaagaggtag atcacgagca acaagggggt gccgcggaac aggtccgtgt
    17761 acacgatcgc gatcaggcgg aacgggaaga acacgggccc gcggagccca cggaccacgg
    17821 cgaccacgag ggcgaggatg aggatcagga cctccgccac caggaagacc tcgatgttca
    17881 gcaggagcgc ccgcccgatc ccggggaggc cttcctcggg cacaccgagg aaggactcct
    17941 tcatcgcctc gaggttgaag aagctctccc tgagagcctc gaagccggga gccgagacga
    18001 cgatgacggc cagcacgccg aagaacagga tcgtgctggc cgtggagatc aggacgccac
    18061 ggttgccgag catccgcgct cgtcgatcgt gcccgggcag agcctccatc ggctccgtca
    18121 tggacaggcc gggtcgacta ctggatcacg gggacggcca agtagtccgc caaccacttg
    18181 ttctggagct gctccagcgt gccgtcggcc gtcatctcgg cgatcgcgag gttcgcacat
    18241 gccacgagcg ggctcccttc ctcgaacaac aggcccaggt gctcgcccgc gctcgggaac
    18301 tgtcccacca gcgcgccgtt cggcgtgttg atgtacgcct cgaagtaggc ggtcgggaga
    18361 tcgaggacga gtccgtcgac cgtcccgttc cggagcgccg ccttcgcgtc gctcgtcgag
    18421 tcgaacacct tggcgtcgtt cgtcggctgg atcaccgtgt tgatgaacgc gagactcgtg
    18481 gtaccgatct gcgccccgaa gatcgcgtcc ttgatctcct ccatcgtcgt tgcgtgctcg
    18541 atcgaggagc ccttcaacac catgagggct tgggtgaggt cgtagtaccc atcgctgaag
    18601 tcgaccgcct ggtcccgttc cggcgtgacg gagatgttgt tgatgtcgaa gtcgaagtcc
    18661 ttcggacccg gcgcgtagga acggttgaac gggatcggga cccagtgcac gtcatcgccg
    18721 gagaagccca tccgggtcgc gacctcgtac gccaccgcgc tctcgaatcc cctgccggac
    18781 tccggcttcc ggttgaggat ccacggcggg aacatgaagt cgaagtcggt tccgacggtc
    18841 agggtgcctt cctcgaagaa gtcggcggag tgggactcgg cgcaggcgga cgccgtcatc
    18901 tcctccgagg gggacgcgct ggatggtgtg ccgtcgtctc cggagacggt tccttctgag
    18961 cacgcccctg cgaccagggc caggacgccc agcgccagga tccatctccg catcggaacc
    19021 ccacccctcg ttgccgtgga agcgcggagc gtagcacagc cccctagcgg cgaggctccc
    19081 agctgaagaa gcgcaccgcg aggatcaggc cggcgatgcc ccaggccgcc acgatcgcga
    19141 cgtcgagcca gtcgaagacg aagacgcgct gtcccgcgac ctcgatgttc ccgaggaagc
    19201 ccgcccgcat cgcgtccgcg aagtgcttca cggggaagac atcggccacg gtcgtgatcc
    19261 actggggcgc ttcgtcgtcc agcgggatga agatcccgga gaggaacagc agaggcagga
    19321 tcgcggcgtt cacgatgggc ggtgcggcgt cggcgttcgg gatcacggcc gtcaacgcga
    19381 gcgcgagcgc ggcgaacgac agggacccga cgaggaacgt cacggtgaat tccaggaggg
    19441 ggccgccgct cgggaggggc gagtcgtaca cgatcctccc gaacacgagc gtgatcacca
    19501 cgaggatcgc gccgacgacc atcgcgtgga gcacccgggc cgccatatag gcgccggccg
    19561 gcagcggcgt gccccggagc cgtttcaaga tcccggcatc gcggttgaac gtcgtctgga
    19621 tcgcgatgtt cgtgaagcac gcggacacga cgccgaacgc tgccatcgcg cccacgtagt
    19681 acgtggtcgt atcgagctcg aaccgctgga gctccacctg gccctggccg agcagcgccg
    19741 tgaagatgac gaggaacatc agcgggaacg cgaacgtgaa gaacgccgat gccgggttgc
    19801 gccagaacgc cttgttcgtg aaccggaact ggcgcccgac ctgcgggacc acgctcactc
    19861 ccgcccctcc tcgccgccgg tcagctcgag gtagacgtcc tcgaggctgg gctgtgtcac
    19921 ctcgagcaga tcgaagacga gatcgtgttc gagcgcccag gccgtgagct cgtggacgac
    19981 cctcgtcggg ttctccgtcc ggatatcgat cgtaccgtcg ggccgcctgg cggtggtggc
    20041 gtggccgggt ggggtcggtc cccccgccgg caagaggaat cggacccgcg ttcgcatctg
    20101 atcgcgtcca cccaaggtcg ccggtgttcc ctcggccacg atcgacccgg aggtgatcac
    20161 ggcgacgcgg ttcgcgaggt actgcgcctc gtccatgaag tgcgtcgtga ggaagatcgt
    20221 cttcccgatc gccgtgaggt tcttcacgat ctcccacgcg ttgcggcgcg cgttgggatc
    20281 gaagcccgtc gtcggctcgt ccaggaacag gagctccgga tcccccgcca gggcgaccgc
    20341 cacatccagg cgtcgttgtt ggccaccgga gagcttgctc acccgcgtgt tccgtttctc
    20401 ggccagcccc acgaggtcga ccacctcgtc cacgtcccgg ggggatggat agaagccggc
    20461 gtacagggca accgtctccc ggaccgtgag atacgggtcc acgccggtcg actgcaggac
    20521 gatgccgatc cggcgcttga gatcggcgtc cgttcgcgcg ggatcgtgac cgaggacgga
    20581 gacctcgccg tcgctccgat cgcgatagcc ctcgaggatc tcaacggcgg tggtcttgcc
    20641 ggcgccgttc gggccgagga gcgcgaacac ctccccccgg tcgacgtgga gatcgacgcc
    20701 ggcgacggct tcgacgtcgc cgtaggactt gcggagaccc cggacgtcga tcacgcgctc
    20761 ggtcgtcacg cgatcacagg ggcttcctgc tcgagagccc ggaccgttcg gagtgatacc
    20821 acccgacgcg ctcttccccg agccgccaac agaggaacac ccgttccccg tccctctcgg
    20881 ccgggaaatc tacgaggccc gcgtcgggat ccctcaggag gatgcctcgg cccgcgagcc
    20941 agaggagctc ccgcttgagc gtctcctgtg cccggaacca gtcgctgccc tcggtcccgc
    21001 caccatcgag ctcgacggcc gaggtgatgc gttcgccggt gtcgatgagt cgctgccgag
    21061 cctcccgcag cctcgggagg cgcgcgcgga gctcctccag ctcggcgtcg gcgtcggcga
    21121 tggagaacag ccgctcctcg gtcatgccgt ggctccgagg atcgacggga gctcgcgaag
    21181 gtccgtgatg cgcgcccccg cgtgatgggg gaaccggccc cggcggtcga cgaggaccgg
    21241 gaacatgccg agtgccgccg gcgggtcgac gtcgaactcg gggttgtcgc cgacgtacgc
    21301 ggactcctcc gccgacacgg acgcccggtc cagcgcgagt cggaagatcc gctcgtccgg
    21361 cttctccacg ccctcgatcc cggagatcac acggacgggg aacagttcga ggagctcgtg
    21421 gacgccgaac caatcctcga gccatgcctc gaagttcgac accacgccga ggaccacgcc
    21481 ggtctcgcgg agcgtcgcca gcgtcgggag cacgtcgtcg aacaggacgt agttctccat
    21541 ccgggtgaac tcgccgtgca gcgaggtcgc gagaccgtcg ccgtcggcga ccccgagctc
    21601 atcgagcatc cgctcgtaca ccgaggtcca gaaggcgcgg gatcgctcgg gcgacgtcgt
    21661 ccacatcgag ccatccctcg ccgcctcgga gaaccgtgcg tacacgtgtg ccgaggcctc
    21721 ccgcacgtcc tgcgcggacc gctcgtggcc cgcgtcggcg aggacggtca cgaagaggtc
    21781 ggcgaaggag ggatcgacgt gcacgagggt ctcaccgacg tcgaagaaga cggcgcggaa
    21841 ccgcatgccc cgaggatagg gcttctgatc actcggctgg tccctcggct gttcctccga
    21901 ctgatccacc gggctcacac gcgagccctg gcctcgagat cgctccagag ccggtcgatc
    21961 tgcggctcca gctccgcgag cgaaccgtcg ttgtccacca ggacgtcggc cacctccatc
    22021 cgctgctcgt ccgtcgcttg ggcagccatc cgagcgcgca cgtcctcggg cgagaggtca
    22081 cgatccgacg ccacccgtga tacccggagg tggggtgacg ccgtgacgat cacgaccacg
    22141 tcgaaggcgg gcgcgagtcc cagctccacg aggagcggac tgacgtagac gaccacacgg
    22201 ccgctgtcgc gaagctcctc gacccgctcg ccgaaccgct tcgcgacctc ggggtggacg
    22261 atcgcctcca gcgccgccct ccgctcggga tcggcgaaga tgatcgcggc gagccgtacc
    22321 cggtcgaggt ccccatcggg cttcaggacg cctggaccga aggtgtcgac gacgcgatcg
    22381 aagcccgtcg tacctcgagc cacggcctcg cgcgcgaggt ggtccgcatc gatcacgacg
    22441 gccccacgcc gctcgagcag gcgggcgacc gtggacttgc cggatccgat cccgcccgtc
    22501 aggccgacca ggagcacgat ccagccccta ctcggatctc ccctcgcggc gcttcagatc
    22561 ctcgaggatc gcctcgagcg agacctcttc gtccgatgcg ggctcctcgg tggcatcctc
    22621 cggttgcgcg gcgacgtcgg gcgcgacggc ggcggcgtcc gccggttcgt cgggtgctgc
    22681 ctcgggcgcc ggctccggtg cggcttccgg ctccgccgtg ggctcgaccg ccgccggttc
    22741 cacggtgggt gctgcagtag ccgccggctc gtccgtctcc gcctccgcga cggcgtcgag
    22801 caccgcctgc gggatctgca cggtctggac gaggggctct gcggctcgct cgggctgctc
    22861 gtgctcgacc tcgatctccg tggtcttggc cggagcgggg gtgacctgcc gcatcgagag
    22921 gctcacgcgc cggcgcgaca cgtccacgtc gacgaccttc acctggacct cgtcgccgac
    22981 ggacagcacc gactccggtg tgtccacgtg ctcgtgggag agctcagaga tgtggaccaa
    23041 tccctcgatc ccctgggcga ccctgacgaa ggcgccgaac ggcacgagct tcgtcacctg
    23101 gccggcgatc acctcgccag ccttgtgcag ccgctcgaac tccttccagg ggtcctcctg
    23161 cgtcgccttg agggagagcg agacacgctc gcggtcgagc tccacgtcca ggacctggac
    23221 ctcgacctcc tggccgacgg tgacgacctc gctcgggtgg tcgacgtgct tccacgagag
    23281 ctccgacacg tggaccagcc cgtccacccc gcccaggtcc acgaaagctc cgaagttcac
    23341 gatcgaggag acggtcccct tgcgtcgctc gcccttctcc aggttctcca ggaacttctt
    23401 gcgaccctcc gactgcgact cctcgaggaa ggcgcgccgg gacagcacga cgttgttgcg
    23461 gttgcggtcg agctcgatga tcttgcactc gagctccgtc ccgatatacg gctggaggtc
    23521 gcgcacccgc cggaggtcga cgagggaggc gggcaggaac ccgcggaggc cgatgtcgag
    23581 gatcaggccg cccttgacca cctcgatcac aggtcccttg atcgtctggc ccgaagccat
    23641 cgtctcctcg atccggcccc aggcgcgctc gtactgcgcg cgcttcttgg acaggatcaa
    23701 gcggccctcc ttgtcctcct tctgcaggac cagggcttcg atccgatccc ctaccttgac
    23761 gatctcgttc ggatcgacgt cgtggcggat cgacagctcc ttggacggga tcacgccctc
    23821 cgacttgtag ccgatgtcga ggaggatctc gtcgcgatcg atcttgacga cgacgccctc
    23881 catgatgtcg ccgtccttga agtcgcgaag cgacgcctcc atcgcgcgca cgagctcctc
    23941 gggcgtgtag tcctcgccct ccggctcgag cgtggcgacg aggggcacct cgtccgtcgg
    24001 cgtcgcgggc ggggccatgt cgccggccgc ctcggtgtcg atctcgctga ccgcttcctc
    24061 gtcgacgatc ggctcagagg tctcgacggt tccgtcgggg acgctctcgg tggtggtctc
    24121 ggtctcgctc atgcggtggt tctccttctg gcacgcgggg ctcaccgcat cgcgggttgg
    24181 gatttgcgac gcggcacgcg aactctccgc gggccgggtg gaagggtagg tgagcagcgc
    24241 aaacgacgtc aatgagcaag ctgcgagacg gtcagtggcc ctcgggagcc gcctccgacc
    24301 agttcgggcc gacgccgatg tccgcccgga ggtcgacgac gagctccacg gccccctcca
    24361 tccggtcgcg gatcaggtcc gcggcctcct ccgcgcggtc cttcgcgacc tcgaagacga
    24421 gctcgtcgtg gaccgtgagc agcatgtggc agtcgagctc cggccgctcc cggagggccc
    24481 cgtcgacgcc gatcatcccc accttgaaca cgtcggacgc gctcccctgg atcggcgcgt
    24541 tcagcgcctg ccgtctaccg agatcgcgca cccgcgggtt ggccgcctgg agctcgggga
    24601 tgtaccggcg ccgtccgagg agggtctcgg tgaacccctc caccgtcgcc cggtcgacct
    24661 ggttctgaag gtaatccttg atcgcgggga agccgtcgaa gtaggcgtcg atgaactcct
    24721 gcgcctcgtc cgccgtgatc ccgaggcggg tggcgaggcc ccaggcgttc atcccgtagg
    24781 cgaggccgta gttgaccgcc ttcgcgcgcc ggcgcatctc cgcgtcgacg tgctcgggcg
    24841 ggaggtcgaa caccttcgcc gccgtcgccg cgtggatgtc gagccccgag tcgaaggcct
    24901 cacgcagccc ggcatccccg gacaggtgcg cgaggatgcg gagctcgatc tgcgagtagt
    24961 cggcgacgag gaggacctgg tcataggcac ccgggatgaa cgctctccgg atctgccggc
    25021 cgagctccga gcggatcggg atgttctgga ggttcgggtt cgaggaggag agcctccccg
    25081 tcgcggcgac ggtctggttg aacgacgtgt ggaggcggcc gtccttcgcg tcgaccagcc
    25141 tggggagcgc ctccaggtac gtggagttga gcttgtccag ctcgcgccac gacagcagcg
    25201 cgtcgacgat cgggtgcgcg tcacggagct tctccaggac gctggcgtcg gtggacagct
    25261 cgcccttggg cgtcttcttc cctggttgga ggcccagctg gtcgtagagg atcgcccgga
    25321 gctgcggcgg cgagttcagg ttgaactcct gcccggcgtg ccggaacacc tctgcgtgca
    25381 gggtggccat ccgatcgcgg accgaccccc ccatgtcctc gaggtacggc acgtcgagtc
    25441 ggacgccgcg cgcctccatc cgcgcgagga cggacgcgag cggcagctcc acgtcctcca
    25501 acagcggccg gagaccctgg cggtcgatct gctcctccag gaccggcgcg aggagggaca
    25561 ccgcggcggc ctccgacgcc acgtgtcgcc agggctcttc cgtgaagagc tgcatctccg
    25621 tctcggtggc tgcctccccc tccacgccga agaggtcggt ccccaggtag cgctcgcaga
    25681 gggccgccag cgggtagtcg gccgtccccg gttccaggag gtaccccgcg agcatggtgt
    25741 cgaacgccac gccctcgagc accgcgccgg cgcggagggt gacgcgctcg agcgccttcg
    25801 cgtcatgggc ccatttccgc gtcgaggcgt cggcgagacg gtcggcgatc gggccgggtc
    25861 cgggaagggc ggcgtaggcc gcctgggctc cgcccgccga gaccgccagc ccgaccagca
    25921 cgtcgtcctc ctcatggatc gcgacaccca ccgggccgtc ggcgaggatg ccggcgaggt
    25981 cctccggctc cacgcgccgg aggtcgagct ccgccacctc gaccttcggc ttcacgtcct
    26041 tggccagctc catcagccgg tcgaagaggc tccggaactc gagcgaggtg aacaggcggc
    26101 ggacctcctc gacgtcccac tcccccatca ccgcgtcatc gggcgtgaag tcgagatcca
    26161 cgtccgtgac gatccgcgcg aggttcttgt tcagggcgag ccgatccccg gcggcctcga
    26221 tgttctcctt cagctttccc ttgagctctg cggtccgacc gaggagctct tcgacggagc
    26281 cgaactgctg gacgagcttc gccgccgtct tgtcccccac ccccgggaca ccggggatgt
    26341 tgtcggacgt gtcccctttc agcgccacgt actcgaggta cttcgacggc gggatgccgt
    26401 accgttcctc gacggccgcc tcgtccatga gcgcgatgtc ggagatcccc cggcggttga
    26461 acaggacccg gaggccgggg cggacgagct ggaagaagtc gcggtcggcg gtgacgatcg
    26521 tggcctcgat cccctgctcg gccgcccgga tggcgagcgt gccgatcgcg tcgtcggctt
    26581 cgtgatcggt gaccaggagc atccggatcc gcaaggcctc cacgacctcc tggatcagcc
    26641 ccagctgggg ccggaagtcc tccggcgtct cccggcgccc ggccttgtag tcagcgtcca
    26701 tcgccagccg ggcggtcggc ttacccacgt cgaaggccac ggcgatcaga tcgggtcgct
    26761 cctccgcgag gagcttgatc agcatcgacg tgaagccgta cacggcgttc gtgacctggc
    26821 ccgtggtggt ggcgagcgtg ctcggaaggg cgaagaacgc ccggtacgcg agcgaatggc
    26881 cgtcgaggat gaagagccgg cggggctggt cgtccgttgg gtccgagggg ggcgccacgg
    26941 cgtcatgcta accgggggcc ccgacgcgcc ggacgggacc gcgaccggga tcggaaaccg
    27001 aggccgagca acagcgccga aggcaagatg attcggtacc gaatcggatt gacgtgaatc
    27061 cgacctcctg actaggattc gcggcgggga caccggacgc gggaagccgc gcgacccgac
    27121 gggaggggat ccatgggagc gaccttcgtc atcgccctcc gcgagggcat cgaggcagcg
    27181 ctgatcgtct ccatcgttct cgcgtacctc aagcagctag gtgcgagcga ccgtgcccga
    27241 ctcgtgtggt ggggcacggg cttggcggtc gcgctgtccg ctgccgtcgg cacggggatc
    27301 ttcgtcgccg gcgcggagtt cgagggcgcg gcggaagagg tgttcgaggg gctcgtcacg
    27361 ctcgcagctg tcggcgtgct gacgtggatg atcttctgga tgcgccggca gggatctcgg
    27421 atcaggtcgg agctccagga gaaggtcgac accgcgatcg tctcgggcgg actcgcactc
    27481 gcgggcctcg ccttcttcgc cgtcctccgc gagggcatcg agaccgcgct cttcctgttc
    27541 gctgccgcga agggcaccgc ggtcgagggg acggcggtcg cgccggccac gcaggtgacc
    27601 ggcgcggtca tcgggctggc gatcgcagtg gtcctcggag tgttgctgta ccgcggcggc
    27661 atccgcatga acctcaggtc gttcttccgg gtgaccgggc tgatcttgat cgtcgtcgcg
    27721 gcggggctgt tcgcctactc cctccacgag ctgcaggagg cggggtggct gcccgtcctc
    27781 gaggcgcacg cgttcgatct gtccgcgagc ctcccggacg acgccggggc aggtgcgatc
    27841 ctgcgcggtc tggtcgggta caacgccgac cccacctggc tcgaggtcgt gggatgggcc
    27901 gcgtacctgg tggtggtggg cgggttgttc ctgcggcgac ccggcgtgcc gctggcgaac
    27961 tctcgacccg aacacgagac ttctgtgacc ggtgcgacgc acgtggagac cgacgccagc
    28021 cgctgagcca accgctggaa cgcgtcgagt cgtgtcactc tcattgcgtg cccgcgacct
    28081 gcggcgatga gaatggaacc ttgctccgca aggattctcg ccgtgcgtcc ctcgggttga
    28141 catccctcaa cctcccgttt acagtccgcg acggttctct acgggacgcg agccccgacc
    28201 ggttccgacc ggccgaggtg gcggtccgag gagaccatcg caacgggccg cgtcaccatg
    28261 aagcggcgct cgcgtatggg gaaggcgcga gcgccacgga ccggagacgc ggcccgttgt
    28321 cgttccccgg atgtctacaa gacggcgtcg aggaaccttc gggtctcggg agagcgggtc
    28381 tcctcgaaga tctgccgagg ggtcccttcc tccaggatcg cgccttcgtg gaagaatgcc
    28441 atccggctcg caacctcccg cgcgaacccc atctcgtggg tgacgaccat catcgtcatg
    28501 tgcatctcca tcgcgagggc cttcatgacc cccagcacct caccgatcaa ctcggggtcc
    28561 agcgccgacg tcggctcgtc gaacagcatg acgtccggtt ccatcgcgag cgcgcgcgcg
    28621 atcgcgacgc gctgctgttg tcctcccgat agccggatcg gatactcccc ggccttgtcg
    28681 gacaggccca cgcggtcgag gaactccatc gcgactcggc tggcggagcc ttcttcgatc
    28741 cccttgaccg tcgccggtcc ctccatcacg ttctgcagcg cggtcatgtg ggggaacagg
    28801 ttgaactgct ggaagaccat gccgcagcgc atccggagct gtctgatctg ctcccgtttg
    28861 gcgcggatcc gatggcctcc ttcgatgcgg agccccgaca cctcgatcgt cccttccgtg
    28921 ggctcctcca ggaagttgat gcaccgcagc agcgtggatt tgcccgagcc ggagcgcccg
    28981 aagaccacga tgacttcgcc cgcgtcgacc tcgatgtcga tccccctgag gacctcgagg
    29041 ttaccgaagg acttgtggag gttctccacc cgaacgaccg gctgaccatc gcgaccactc
    29101 atggcggatc ctcgtccggc tccggcaccg tcggcagagc acctccatgc gcatgaccta
    29161 cgaggccgac ctcctcctca ggtgcgtcca cgacgtagcc ggccgccgcg ccgtgctcac
    29221 caccgccccc cgcccccgtg acgatcacct tccgccgctc tcgaaccttg gggcccgcga
    29281 ccgcgcggac gtaccccttg ttcacgcgac gctcgagccg ggactggaag taggtgaaga
    29341 tggcagtaag tgcccaatag agccccgcag ccgtgatcag tgcctccatg ggcctcacgt
    29401 ccgcgttccc gatcagctgg gcccgacgga agagctccat cgattcgatg gattggccca
    29461 ggaccgacac cagggcggtg tccttcatca tcgcgatgaa ctcgttgccc gttggcggga
    29521 tgatgacgcg gaatgcctgt ggcaggacca cgcgtcgcat cttctgccgg tacgtcatcc
    29581 ccagggcctc ggccgcctcg ctctgtccgt gactgaccga ctggatgccg gcgcggaaga
    29641 tctcggtcat gtaggcgccg tagttcaagc cgagcacgag gagcccgacc tggaaggcag
    29701 ggcgcgtgag gatgtccacg aggccaccgg ggaggtcggg gaacctcgcc gtgaggttga
    29761 tcccgaggcg cgggagcgcc aggtagaaca ggaacagctg gacgatcagc ggggtaccgc
    29821 ggaagaacga ggtgtagaag ccgctgacgc cgtacgcgac cgggttccgc gacaatcgcc
    29881 cgagcgctcc gaggagagcg agcacgacgg ccaggatgat gccgcccgcc gccatcccga
    29941 gcgtgaagac gaggccccgg gcgatgaagc cgatgtgctc tctgatccat tggttgtcga
    30001 acgacgaggc ccagaagagg aacccgagca aggcgaggat cacgagccag acggcggcga
    30061 ccttcacgcg gaaggaaaca cccgcgctcg cgagagcgag tcgatccctc cgtcgttgta
    30121 tgggagcgag cgggggccgc gccgcagccc ccgcctcgcc cgtgtccatc tcacgccctt
    30181 cgtcgaggtg ctacgcgacc tcggagacgt ccgctcccgg ataccaggtc tcggagaagc
    30241 tcgacagcgt cccgtcctcg tgcatgtccg ccacgatgcc gtccacggtc tccaccaggg
    30301 tggtcccatc cagctccgat gacttgtcga acgcgaccga cagcggttcc ccgaacagcg
    30361 gctcccccac gatcttcacc gggttgccgg cttcgacgaa ctcctgggcg atggagctcg
    30421 ccgtcatcac cgcgtccact cgcgtcccgt ccccgagcgc gaggtcctgc aaggcggtgg
    30481 tgtccgtgtc gtagccggcg acggtcgcgt cgtcgatcac gaagtcgaac tcgaaaccgg
    30541 agatctccag ggtcttctcg atgaagaact gataggtgca gtcggcgcag acgccgatcc
    30601 gcttgccgtc caggtcggtc gtgagatcgg agatcgtcgt gttctcctcg tgaaccacga
    30661 ccacggccgg gacgaaggcg tagggctcgg tgaagttcag gacctcctgg cgatcgttgg
    30721 tcggcgtcat cgacccgacg ctcatgtccc agcggtcgtt ccagctcccg gcggtgagga
    30781 cctcccagtc cggggtctcc cactccacct tgacgccgag acgggccgcg atctcgttcg
    30841 cggtgtcgat gtcgaacccc tcgtactcac ccgtctcggg gttcaactcg gacgccggag
    30901 gataggcggg atcggtcgag acgcggatca cgccttcggt gcagatcttc gcgagcaggt
    30961 caccggcctc cttgtcgtag gtggcgcaga cggtctcgtc ggccgggtca tctccgttcc
    31021 cgcctgccgg cggggcgggg tcctcgccgc agccggcagc gatgagcgcc aggaccccca
    31081 cgagcaccgc gatccgtcgg aaaccgacct tcatgagatc ccccttgggt cgatgagtgg
    31141 gcgccctcga tcgcgcgcgc ccttcggccg gactctaggg caggaccggt cgccgggcaa
    31201 cgcagtcagg cgggtgctcc ggtgagaggt tcgaccggca gccagcgaga tcccgtggca
    31261 cgccacgcgc ccacccggag ggtttccggt gccctcgatc cgtccgcctc gaagcggtag
    31321 ctgcccgcga ggccggggat ctcggccggt tcctcgagcg cagccgacag gatcccccgc
    31381 ggatcgtcgc ttcccgctcc ggcgacgagg tccaccagga accggccggc gtcgtacgcc
    31441 tccaccgagt acggaccggg cggggcgccg ctcgccgcct ggagatcgtg cacgaaccgc
    31501 atgtggtccg gctccaggga caggttcacg ctcacacagg cgcacacggt gatcacgtca
    31561 cggccagcgg tggcgagcgc cagtccgtcg tccgttccga tcctcgaggc accgaccacg
    31621 agcgccgggc tctcgggcaa ccgggagagg agcgcctccg cgccggtggt cccgcccgtc
    31681 cacgcgagga ccggacatct ctcctcctcg atccggtcgg cgaccgggtc tgcgatcggc
    31741 acgtcccctt cggcgaccgt gccggccatc acgatgtccg ggtcgcccgc cgctcgagcc
    31801 agctccgcgg ccgtcgcgag gagggcgcgg ctggtcgggt gctcgtcgcc ggcgaggcac
    31861 agggtcgcgt cctccggagc cacatcggcc gtcgacagca ggagcacggc ctcctgtgcc
    31921 cgccccgccg ccagcgagag ccagaccccg tctccgtcgg agggtggacc ccacgcccac
    31981 gagagcgaga cgacagggag gccgtgcgcg gccagcaggt cgatcgcccc ggccgggggc
    32041 gctgtccacg gcgcgatcac ggcggcgacg acggcgctgt cctccgcgag agctcccagc
    32101 gtgctcatca cctcggtgcg atcgaggccc aggttcaccg gttcgatctc gacggcgccg
    32161 gcctcatggg ccgcgagctc cacgcccgcc agtgccgacg cgctcacgag ctcggcgtcc
    32221 ggaggcgacc cgtcgaacag gtacgcgatg gtgggccggg agggatctgc tctcggcgga
    32281 agacctcccg tgcacgccgc gaccgagagc gcgagcagcg ccgccaggcc ccggcggatc
    32341 ctctggctcg ctcggatcct ctggcccgct accctagacc ggcccggccg ggatggcgga
    32401 acaggcagac gcgcgggtct caaaaacccg tgggcttcgg cccgtgaggg ttcgaccccc
    32461 tctcccggca cccagaccct cccgctccgt gccgtgcacc tgcgcgcccg catctcctgg
    32521 gattatcgat gcgatcgctc gtgtcgatca ggtggatggc gcgtcgacga tcgcgcgggc
    32581 gacgaccagg tcctcgaacg ccatccccac gctcttgaag acggtcaggt cctcggtcga
    32641 gcggcggacc tccgcacccc ggactgcttc cgcgaggtcg gcggccacgt gtcccgcctc
    32701 gatcgccccg tcggcgatcg ggatcgccag ctcgcccgcc tcggcgagcg ccacctcccg
    32761 ggtctcgacc acgaccctcc cccgtcgcac ggtctcggtg tcgagctccc tcgcgtcggg
    32821 acgatgggat ccgaccgcat tcacgtggac gccgggcgtg agcagcgatc cgtcgaacag
    32881 cggttcccgg gaggtcgtgc acgtgcagac cagagccgcc tgggcgaccg acgccggctc
    32941 ccccagtcgc gccgccaatc ctcgctcgag gccggcctgg acgagggatc ccgccggtcc
    33001 gggggtacgc gagacgacga cgagctccgt gaccgggcgg accgcacaca tcgcgtcgac
    33061 gtgcgctcgc gcctgcacgc ccgccccgaa caccacgagc cgcgccgcgt cctcccgtgc
    33121 gaggtgccgc gtcgccaggc ccgagaccgc cgctgtccgg agcgccgtga gagccgtccc
    33181 gtcgaggacg gcctccggtg actgcgtgac ggtgtcgaac agcacgtaga cggcctggat
    33241 gtacgggagc ccgcgcccgg ggttggacga cgtgagcgtg accagcttca cgccgacccc
    33301 cgcctcgccg aaggccggca tcacgaggag cgaccctgcc ggcgtctcga ggctggtgcg
    33361 gagggggccg gtccccggat cgagcgtgcg gaacgccgtc tcgagggcgt cgatcgcggc
    33421 gaccatcggg agccgggcgt ggagctcgtc cgcgccgatc agccgcagct cagcgcccac
    33481 ccctcacagc tcgccgaggt aggcctcgcg catcaacggg ttctgcaaca ggttctcggc
    33541 cgtgtccgcc agcaccacct ggccggtctg gatcacgtag ccgcgatcgg cgatcgacag
    33601 ggccatgttg gcgttctgct ccacgatgaa gaccgtcttc ccgagctcgc ggatcttctt
    33661 gatgatctcg aagacctgct cgaccaggat cggcgcgagc cccatcgagg gctcgtccat
    33721 gagcaggacc tttgggtcgg ccatcagggc gcggcccatc gccaccatct gctgctcccc
    33781 gccggagagc gtgcctgcct tctgcttcaa tcgctcgcgg acgcggggga acgtctcgaa
    33841 cacggtctcg cgtgcggcct cgaccgatga acgatcacgg cgcaggtacg ccccgagccg
    33901 gaggttctcc tcgacggtca tgtccgggaa cagtcgccgg ttctccggaa ccatcgagac
    33961 cccccgccgc acgacctcgg tcgtcgacat gcccgacacg acgttcccgt cgaggaccac
    34021 ctcgccctcc gacggtgtca cgtagccgag gatcgtcttc agggtggtcg tcttcccgct
    34081 ggcgttgcca ccgaggaggc agaccatctc tccggcgtag atgtccatgt cgacgttccg
    34141 taggaccgca accagcccgt agtgggtcga gacgttccgg agctccagga tcggtgcccc
    34201 gcgcacggcc gcctcggccg tcatccggcc gccttggttc cgagataggc ctcgatcacc
    34261 cgctcatcgt tggcgacctc ttcataggtg ccctcggcga tcttctgtcc gtagtcgcac
    34321 gcgacgaccc ggtcggagac gcccttgacc acccgcatgt cgtgctcgat cacgaccacc
    34381 gtgagcccga gctcgtcccg catgcgcacg atgtgatcgc gaagctcgag cgtctcgcgc
    34441 gggttcatgc cggccgtggg ctcgtccaag aggaggagct gggggtcggt cgccatcgcg
    34501 cgagccatct ccagccggcg gcggttcgcg taggagagag agaacgcggg ctgttccaga
    34561 cgatagccgg agaggcgttt gccgaagaag ctgagtgcct cctcggcgcg ctgccgtatc
    34621 cgctcctcct cggcgcggac gcccggcgtg cggacgatgg acccgaagac gccgcccttg
    34681 ctccggcagt gctggcccac catggcgttc tcgaggatcg tcatgttcgc gaagagccgg
    34741 acgctctgga acgtcctcgc gatcccgagc ttcacgacct gatggggtcg cagtcccacg
    34801 atggtcgagc cctgataccg gatgtcgccc gcgtccggct cgtagatgcc cgtgatcacg
    34861 ttgaagagcg tgctcttccc ggcgccgttg gggccgatca ccgacacgat ctcgccgcgg
    34921 tgcacctcca cgtccaggcc ggagagcgcg tacaggcctc cgaagtgctt ctcgagacca
    34981 cggacctcca gaacggcgtc cgcgctcccc gatgaggcgg cgtcgctcac gtcgtcacct
    35041 ccaccggcgg cccgagctca ccgggggtct ccccctcttc cacggctttc gcccattcgt
    35101 cctgggcacg ttcctcatcc tgcagctcgc ggctgcggcg tacgttgggg accaagccct
    35161 gcggtcgaag gaccatcacg gccacgacgg ccgcgccgta cgcgaggaac cggtactcct
    35221 cgaactcccg caggagtccc ggcagtccca ccaggacgag tgctccgacg agcacaccgg
    35281 ggatgctgcc caggccgccg aggatcacca gcccgagcac ctggatcgac acgatcacct
    35341 ggaagctcgc cggcgtcagc gatccgatct tcacggcgaa gagcgcccct ccgagcgagc
    35401 cgatcgcgcc gccgatcgcg aacgcgagga gcttgaagcg cgtcgtgctg atccccatcg
    35461 cgtcggcggt ctgctcgtcc tcccgcatcg cggtccacgc ccgcccgatc cgtgacgtcg
    35521 cgagtcgcca cgacaggaac gccgccaggg ccacgaacgc gagcgcgagg tagtagaagc
    35581 gctgcgggtc gcggaacgcg aaccctccga tcgcggcatc cgtgatcccg cgcatcccgc
    35641 tcggtccccc cacgagcggg acgagccagg ggctctgtac gagcaccgtc accatctcgc
    35701 cgaggccgag cgtcaccagc gcgaggtagt ccccgcgcag ccgcagcacc ggggcgccga
    35761 ccagcacccc cgcccccgcc gcgatcagca tcacgatcgg gatcgcgacg tagaagttca
    35821 ggccacccga gatggcgggc tcgggcgcga tcccgtatgc gttcagggtc gctcccgtga
    35881 gcagcgcgag cgagtaggca ccgagcgcga agaagaacac gtagccgagg tcgagcaggc
    35941 ccgcgtaccc gacgacgatg ttcagaccga tcccgaggag ggcgaacacc atcaccgtgc
    36001 cgagcacctc ggagatcacg gatccgagca ggagcggcgc ggcgatcagg agggcgacgg
    36061 cggccacgct cgcgatcagc cgtacggggc tcatcgccgg cccgctccga cccccgatac
    36121 ccgacgagag gctcccgcgg acccgtccgc cgtaccgcgt gttcagaagg ctcacacccg
    36181 cgctgactgc ggccaccacg gcggcgccga tccaggtcag tccgtcatgg atcgggtggt
    36241 acagccagtc acgctccagc gacagctggt cgagggcgat cgggatgacc cgttggagga
    36301 aaccgaacag gagcgtcacc gtcacggccg cggtgaccga ccggcggacg accggcgggg
    36361 ccacgcggag gaggccaccg agcacgcccg cgatcgtcga cagcgcgacg aggatcacgg
    36421 cgccgaccac cgtcgactgc ccgaaggcca cgaagtccat caggctcggc gacacgttga
    36481 ggaagatcgg acggatgcgc tcgtccccga tcaggtccgc caggagcacc gcgagggcga
    36541 acacgacgcc ggcggatgcc gccgcgacgg ccgcggcgac ggccgcctcc ccgagcggca
    36601 tctcgcgccg ctcgccgccc tggatccggg gcgccgcggc cacgaacccc gcgaggaacg
    36661 cggggaggag caacatcacc ccggcgaacg tgatctcgtc gccgatcagg aacacgtcgg
    36721 tgaagccccc gatcaggccg acgagggcga ggaagatcgt gaccacgccg gcgagcgccc
    36781 cggtccgggc ccagcgtcgc gccgccgtca caccttctcc gcctcgccgg tgccgaggag
    36841 tccccccggc cggaagacca ggacgaacac gagcacgagg aacacgaacg cgtcccgcaa
    36901 cgagaagacg ctcgggatcc cgaaccccgt gaggatcagc ggcggcccga ccgcctggag
    36961 cagcccgatc acgaacccgc cgagggcggc gccgccgatg ctgccgatgc cccccaggac
    37021 ggcggcggtg aacgccgcga tccccggccg gaagcccatc gtgaactgca cctgctcgaa
    37081 cgtgagcgag aagagcactc cggcgatccc cgccaggacc cctccgatcg cgaacgtgac
    37141 gacgatcacc cggtccacgt tgatgcccat caggctggcg atctcgcgat cctgggcgac
    37201 cgctcgcatc gatcgccccg ttcgcgtcgt cgccacgaac cactgcaggg cgatcatgca
    37261 gatcagcgcg acgacgagta cgagcagctg ggtgcgttgg accggcaccc ccaggaccgt
    37321 gacgtggccg gccagcacgg tgggcttcgg atacccgtac ggctgcgggc cgaagaaccc
    37381 gcggaacgcg ttctggagga agagcgaggc accgatcgcg gtgatcaagg ggacgagccg
    37441 gggcgcgttc cggaggggac ggtacgccag ccgctccagc aggaccgccg tccccacgga
    37501 gatcgccacc gccacgatgg tcacgatcgc cagcgccagg agggggctcc ggttcaggaa
    37561 accgctcttg tggtaggcgt cggcgaagaa gaacgatccg aaggcacccg ccatgaagac
    37621 ctcgccgtgg gcgaagttga tcatccggag gatcccgtac acgaggctgt agccgagcgc
    37681 gatcatcgcg aagatcgcgc cttgcgccgc gccctgcacg atgaagtcgc gccacgcgcc
    37741 ggccgagtat ccgccctggg cccgatcgag cccgagggta cgccatgcgc cgaggacgac
    37801 cacgccgccg acgacgatcc ccaggacgac gaggatgatg cgccgccacg gcaatcgctc
    37861 gaagcgaccg agccgagcta ccgccacgtg cggggcctcg gtgcccgcca gggttccccc
    37921 tctcagggaa ggggccacgg gcgagcctct cggctcggcc cgcggcccct ccgccgtggt
    37981 tcgtggatcg ctagaggact tcgtccagcg acttggtgtc ggtgtacacg gccggatcgt
    38041 ccgtgccgcc ctcgaccggc cagccgggag cgaggaacag gccgatcgtg acgtccgtcg
    38101 cgcaatctcc cgtatcgagg cacgagatcg tgcccgtgac gccctcgtag ccgtccgtgg
    38161 cgaagatcgc gtcacgcagt gcctggcgag ggatcgagag cgatccgtcg ccgttgtcga
    38221 tcgcgacctc ctcgatcgct gcgaacacga cgttcgccgc gtcgtaggag tgcgcgtgga
    38281 acaccgcgag cggctcggtc ccgaacgcct cctgatacgc agggatgaac tcacccgagt
    38341 agaactcgtt cgtctggaac gccgacaggt ccggcgacga tgcccagacg ccatccgccg
    38401 caggtcccgc gttcttcagg gtgtcgctcg acaggcaccc gtcggccacg atcagctcgg
    38461 cgtcgggcat gatgtcctgc gcctgcctga tgatgagcgt gcacgctgcc acgaagatcg
    38521 ggaagtacag gacgtccgga tcctgttcgg ccaacgacct gaggaccggc ttgaagtcgg
    38581 tgtcttcgga gttgacctgc tccgatccgg tgagggtgcc acctccggcc tcgaagttcg
    38641 cctcgaacgc accgacgagt ccctgggtgt aggggctctc gtccgcgatc gtcgcagccg
    38701 tggtcgcgcc gaggtcttcg cccagcgcga actcggcgcc gatcgcaccc tggatccggt
    38761 cgttgtgggc ggtccgggcg tagaacgggt tgtgcagctc gggatcggtc agcgcggggc
    38821 tcgtgttcga gcccgagatg agcgtgatgc ccttgtcgcc gaggatcgtg tccgcgacgc
    38881 cgagcgccgc gctcgagcag ctcgttccga ccaccgcgac gatcgaggtg tcggcggcga
    38941 gcgccgtgcc cgccgtctga ccgccttcgg ccgagcacag ctcgtcctcg accgtgagct
    39001 cgaccgggtg gtcgagcaac gtgcccgggg tctggtcgaa cgactcgtcg aggtagtcga
    39061 tcgcgagatt gatcgcgttc acctggtcct gaccgagcga ggcatccgcc ccgctgatca
    39121 cctgcgcggc gcccaggttg atcggctcgt ccgcgcccac ctcgacgcat ccgaactcga
    39181 cggtggcaca gtcgacctcg cccgcgcccg gccctccgtc gccaccgccg tcgtcgtcgg
    39241 cgcacgccgt cgcgacgatc gtgagcactg ccacgagggc gagcgctttg aaccatatgc
    39301 ggtgcttcat ggagcccccc ttctcctcgg tctcccgaga ttggatgtga ctatcacatc
    39361 gcggccacgg ttggccaatc gccgccgtgt ccgcaagggc atacgtccac accgggaggc
    39421 ggagatgcct caggagggga tcgcctcgcg gaacgcggtc gccagctgga tcgccccttc
    39481 ggcgtcccca cccacgatcc tgtgcatcaa cgcgctcccc acgaccacgc cgtccgcgaa
    39541 cgcacacacc tccgcggcct gtgcgggcgt gccgatcccg acgcccacga gcaacggcag
    39601 gtccgtgtgg aggcggagcg cggcgacgac cgcccccgcg gtctcctcca cgcggtcacg
    39661 ggcaccggtc acgccgtacg tggccacgca gtagacgaag ccgcgtgacc tccgcccgat
    39721 ctccgccatc cgcccctcgt gggtccccgg cgccgccagg agcacggcgt cgacgccgcg
    39781 agcggcggac tcccgcccga ggtcgctcga ctcgtcgacg gggaggtccg ggacgatcac
    39841 gccggtcacg ccggcggcaa cggcctcgtc gaggaaggac gagatcccgc ggcgcaccac
    39901 cggattcacg tacgtcatga ccgcgacggg cacctcgatc gcggcctcgc ggatcgtggc
    39961 gagaacgtcg ccgggccggg cgcccgcgcg cagggcctcc gccgaggcct cctggatcac
    40021 gccgccgtcc atcacgggat cggagaaggg gatgccgacc tcgatcgcgt ccgctccggc
    40081 ggccgcgagt cggcggagga gaccggcgtc cacgcccgga tacccgccgg tcacgtacgg
    40141 gacgaacgcc tgtcccccgg cgtcgcgccg ggcccgcagc gcatcctcga gccggcccat
    40201 ctacgagatc ccgagactcg cacgcaccgt gtcgaggtcc ttgtcgccgc gccccgagag
    40261 gcacacgagg accgacgtgc cctgctccag cggtcggtgc agcagccacc cgatcacgtg
    40321 cgccggctcg agcgccggga tgatgccttc gctcctggag agcgcctgga acccgtcgag
    40381 cgcgtccccg tcggaggcgc tcacgtactc ggcgagtccg gcgtcgcgga ggtaggcgtg
    40441 ctccggtccc acgcccgggt agtcgagccc ggccgagatc gaatgggtct gcgcgacctg
    40501 gccgtcgtcg tcctgcagca ggtagctcat cgccccgtgg aggatcccgg gatgcccctg
    40561 cgtgagcgac gagcagtgct cgccgagccc gggaccgcgg ccgccggcct ccacgccgat
    40621 cagccggatc cccggtgcac cgacgaacgc ggtgaacatc ccgatggcgt tgctgccgcc
    40681 gccgacgcag gccgcgacca cgtcggggtc ggcgcctccg gtcgcgtcgc tgaactgggg
    40741 gcgtgcctcg tcgccgatca cgcgttggag atcgcgcacg agcaccggga acgggtgcgg
    40801 ccccacgacg gagccgatga tgtagtgggt cgtccggacc gatgccgccc agtcccgaag
    40861 cgcctcgttc accgcctcct tgagggttct ggagcccgcc gtgaccggga cgacctcggc
    40921 gccgaggagc ttcatccgcg acacgttcgg cgcctgtcgg cgcgtgtcct cctcccccat
    40981 gtacacgacg cacggaagcc cgagcagggc gcacgcggtc gcggtcgcga cgccgtgcat
    41041 gccggcgccg gtctcggcga tgatccgctc cttccccagc cgtttcgcga gcaacgcctg
    41101 tccgagggcg ttgttgatct tgtgcgcgcc ggtgtgcgcg aggtcctcgc gcttcagcca
    41161 gatctcgtag ccgagctgtg tggacagccg gtccgctcgc gtgatcggtg tggggcggcc
    41221 gacgaaggtc tccgacagcg tcgagagctc cgctcggaag ccggcgtcct cccgcagctc
    41281 ggcccatgcc tcggcgagct catcgagcgc ggggatcagc acctcgggga cgtagcgccc
    41341 gccgtaggtg ccgaaccgac cgcgtgcatc cgggagcgtg gtggcggtca cgtgcgcagt
    41401 tccctccaga gcagcttccg cacggcacgg gcagggtcct ccgcccgcat cagggtttcc
    41461 cccaccagga tggcagacgc cccggcggcg agcgcggcgt ccacgtgccc gcgggtcgag
    41521 acaccgctct cgagcacgcc caggcgatct gcaggcagcc cccccagacg tgcgagcgcg
    41581 ccctccacat ccacgtcgag cgtttcgaga tcgcgggcgt tcacgccgat caccggcgcg
    41641 tccgtgtcga ggacgcgccg gagctcctcg tccgaatgcg tctcgacgag ggtcccgagc
    41701 cccagatctg ccgccgcctc caggagcgcg cggagttcgg catccgtgag cacggccacg
    41761 atcagtaaga ccgcgtccgc tcccgatgcg cgtgactcga tgacctgtgc gggatgcacg
    41821 acgaagtcct tgcggaggac ggggatcgac actgacgaac ggaccgcctg cagatccgcg
    41881 agcgatcctt ggaagtggcg aggttcggtg aggacggata cggccgccgc gccgcccgcc
    41941 tcgtagccgc gggccagcac gctcgcgttc gcgtccttcg cgatggcacc ggcggacggg
    42001 gacgcccgct tcacctccgc gatcagcgcg ggcggggtgg atccccgcaa cgcaccctcg
    42061 aagtccctcg tgggaggcat ggccgcggcg cgggccatca gcatcgcatc gtcgagcgga
    42121 tgctcgcgga ggtcctgtcg cagccggtcg acgaggtcgg tcaggaagcc catggggcgg
    42181 cgagtctaga cgtggtcgct caggcgaggg ccgagcgcgc ttcggcgaga tccagggtgc
    42241 cctcgagggc ggctcgcccg accaccgcac ccagcgcgcc ggccgcccga agggctcgga
    42301 gatcgtcgac ggtgtggatc cctcccgcgg cgatcaccgg gcggccggcg cgcacgaccc
    42361 tcttcacggc tccgacgtcg ggcccgatcg acgaacccac cctggccacc gccgtgacga
    42421 ggaacccctg agctcgggcg gcggagagcc atccgagcgt atccatcagc ggcaggtcga
    42481 cggggtcgcg gccccgcgat cggatcgtgt cgccgtcacc gacctcgatc ccgacgatca
    42541 gccgcggccc gtgcactccg aggaggtccc gtgccaggac ctcgtccgcg agcgccgccg
    42601 atccgagcac gacgcgttcg acacctacgg agaggagggc atcgacctct tcggcggttc
    42661 gaacacctcc gcctgcttgc acgcggaccc ggagagagac gatcgccgac acgacgtcga
    42721 ggttccgtgg ctcgccgtcg aacgcgagat ccatgtcgac cacgtgcagc cgagtcgcgc
    42781 ccgcctcgac catcgcggcc gccgccgcga ccgggtcgcc ggcgaacgcg tcgatccgca
    42841 tcagccccgt cggcgtcaga tgggcgagcg ctccgtcctt gacgtcgatc gcggggatca
    42901 cctcgaacac gcgggcacga tagagcctgc gccgtcgggc gatgggtccg tgcggcctgg
    42961 gcgccgggct gggctcaggt gacgcgttcg acgcgggcgg cgccggagcc cagcagtccg
    43021 gccagctccc ccatcaatcc ggacggatcc acgaggaact cgcccatgtc cagcgggcgg
    43081 acgccatcga tcgaatggaa ccggacccgg accggggccg agccgggacg agaccgcagg
    43141 agctccgcca tcttcgaaag caccgcgtcc gtgcacgcag ctgccgtgag gtcgatcacg
    43201 accgactccc cgcccgccga cggggccgac cccgtcagga tcggctcgcg cacgtcgttc
    43261 gcgcggatct ggagctcccg accgcgccgg tcgatccgcc ccaccaccag cacgatccga
    43321 tcggaggcga tcagatccgg gtccgcctcg tagacggagg ggaaggcgac gacgtcgacg
    43381 ccggcggaga ggccttcgag gcggaactgc gcgtacggct ctcctcgctt ggtgtacttc
    43441 ctggagacac cgccgatgat cccggcgacg gtcaccagct ctccgtcgtc cgcgccctcg
    43501 aggtcgccga tctcgtgcgt gctctgggcc cggaggtcgt cggcgacctc cagcagcgga
    43561 tggtccgaga cgaacgaccc gagcatctcc ttctcgaaac gcaagagctc cggcttgtcg
    43621 aactcctcgc cctcgaggac tgactcgtcg atctggccca cgtccgagtc gccgccgaag
    43681 agcgagaact gtccggcggc ctcggccttc cgctcggcga cgatcggacc ggacaccttg
    43741 tcgtgcccga tcaggagggc ccggcgttgg tagcccaggg agtcgaaggc acccgcgagc
    43801 atcatggact cgagcgcgcg cttcgtgagg acggacgggt cgaccttccg gcagaagtcg
    43861 gagaaggacc cgaacctgcc cttggcggcc cgcgcgtcca tcacctgctg cacggccgcg
    43921 tcgcccacgt tgcgcaccgc cgacagcccc tagcggatcg cctgatcgtc gtcggtggcg
    43981 ggggcgaagt cgagcgtgga gtcgttcacg tccgggggga gcacctcgat gcccatcgca
    44041 cggcatgcat acaggtagta gggcttgcga tccttgtcgt ccttcacgga t
//
LOCUS       DASUYD010000180         7415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_330253, whole genome shotgun sequence.
ACCESSION   DASUYD010000180 DASUYD010000000
VERSION     DASUYD010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330253"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            367..882
                     /locus_tag="VF195_10195"
     CDS             367..882
                     /locus_tag="VF195_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581227.1"
                     /translation="MRRTIGLSIAGALLMIAGFVGPVEAAGVQRSTTGDGRQLTGAGV
                     GFRTFAFAVVEHNDGRVTGEASVMNPVIGSDRVFEISCLTVIIGNDGSPIAIASGPVV
                     AAEDPSLIGRNGIFAVQDLGEGHDPSDRMTLGVLLSPVRCADVTSEEALRSVFPRGLL
                     AIDDGNIQVRA"
     gene            1033..1584
                     /locus_tag="VF195_10200"
     CDS             1033..1584
                     /locus_tag="VF195_10200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581228.1"
                     /translation="MLNEPAAPGIHAAVQTDDNVVDLHGERETSATPLSFDDLFLDHH
                     DRLYRALYFITGSSADAEELMQDAFLKLWERWDRIHTIDDPVAYLFRIAMNGFRMRAR
                     RARVAARRIVHLEPTRDPYDDIELEDDVRRMLLGLPPRQRAALVLTEIFGYSSEQAAR
                     ILGIRATTVRVLASQGRATLRAR"
     gene            1595..2371
                     /locus_tag="VF195_10205"
     CDS             1595..2371
                     /locus_tag="VF195_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581229.1"
                     /translation="MPDVKELYELVTKQRPPDTGAFERQRTRQIRTTRNRKVGAFAVV
                     AAIGVAAAAVILGTPRSSEPKREVGGRPPVSPVEVATSFVEAYGALDVDTVIGLLGDD
                     ADISPLIDWVNLRTGGPLEELRMTFSLLEAQGYSHTRDSCEEVSRSASNTSLRCTFEL
                     HWLHSDEIGRGPFAGSYFDITVRDGEITRVSNYVEIEEFSPQMWEPFAAWVSTNHPRD
                     VLDMYEDETQSLERVTEESIRLWDRYTRDYVEEVNQGVIG"
     gene            2415..3083
                     /locus_tag="VF195_10210"
     CDS             2415..3083
                     /locus_tag="VF195_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581230.1"
                     /translation="MERITREPRTAGCLSSPLATSSRVARILIISVILTAGLPASAGV
                     EAGLADPAGDAEHGAPEFMDIVGAEASKSGQVFRFQMSLAAPIPAVPPPTPPGTNQIE
                     WDWSLNTDPTTFPAGSPFPGGPGQGRPAEFIIHVVWDGRSFSAHLTDRRPLLSGGEAF
                     ITPLVFTSSGTTTWVDLSASVLDKPSTFLWGAVTFYWSSPPGGTAGGHFVDSLEPFYN
                     PFPA"
     gene            complement(3278..5767)
                     /locus_tag="VF195_10215"
     CDS             complement(3278..5767)
                     /locus_tag="VF195_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEX6581231.1"
                     /translation="MFERFTDRARRVVVLAQEEARMLNHNYIGTEHILLGLIHEGEGV
                     AAKALESLNISLEAVRQQVEEIIGQGQAAPTGHIPFTPRAKKVLELSLREALQLGHNY
                     IGTEHILLGLIREGEGVAAQVLQKLGADLNRVRQTVIQLLSGYTAGKGEQVGASQEGG
                     GQGSMVLDQFGRNLTQLARDGKLDPVIGREKEIERVMQVLSRRTKNNPVLIGEPGVGK
                     TAVVEGLSQDIVREEVPETLKGKQIYTLDLGALVAGSRYRGDFEERLKKVLKEIKTRG
                     DIVLFIDELHTLVGAGAAEGAIDAASILKPMLARGELQTIGATTRDEYRKHLEKDAAL
                     ERRFQPIVVEEPTVSHTIEILKGLRDRYEAHHRVSFTDDALVAAANLADRYIADRSLP
                     DKAIDLIDEAGSRTRIRRMTAPPDVREVDEKIAEVRLRKESAIDAQDFEGAASLRDEE
                     RQLQEERQRREQRWKAGEMDVLSEIGEEEIAEVLSVWTGIPVFKLTEEETEKLLRMEE
                     EIHKRIISQHESISAVSRAMRRTRSGLKDPRRPSGSFIFLGPSGVGKTELSKVLAEFL
                     FGDEDALIQLDMSEYMEKHTVSRLIGSPPGYVGYEEGGQLTEAVRRKPFAVVLFDEIE
                     KAHPDVFNTLLQILEDGHLTDAQGHKVDFKNTIIIMTSNLGTRDIAKGVGIGFTAARD
                     DAVTYTKMKENVTEELKRSFRPEFLNRIDEVIVFHSLTPDDVKQIVDLLMRRVEEQLK
                     SKDVDIVLTDAAKTLLAERGYDKALGARPLRRTIQQLVEDPLAEKLLYKEFRAGETII
                     VDAHDGEITFEHGAGIMPPDTPPVELAGSTE"
     gene            6187..6573
                     /locus_tag="VF195_10220"
     CDS             6187..6573
                     /locus_tag="VF195_10220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581232.1"
                     /translation="MEKESETVRVLVVDDHPAFRKALASALQLVGDIEIAGEASGGED
                     ACVGAKELQPDVVLMDLSMPDISGIEAMQRIHSSTPELPVVILTAHADEGVERAARGA
                     GASGFVAKGRGLQDILRALHEVTAAR"
     gene            6596..6883
                     /locus_tag="VF195_10225"
     CDS             6596..6883
                     /locus_tag="VF195_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011936201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581233.1"
                     /translation="MGCLIVLFVLLLPRTIMVALWLFSSYLSTAFGTWVWPLIGFFVL
                     PTTTLAYAVAQNRYGGVRGLGLVLVILGVLVDLGALGGGGRGAGKRWAGDR"
     gene            complement(7131..>7415)
                     /locus_tag="VF195_10230"
     CDS             complement(7131..>7415)
                     /locus_tag="VF195_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX6581234.1"
                     /translation="FDLIIGGVELVTAFSELTDPLEQRAKLELQQQLKAAGVEETHPL
                     DEDFLRALEHGMPPAGGLGLGIDRLLMVLTDTASLRDLIMFPAHRPEALD"
BASE COUNT         1150 a         2451 c         2495 g         1319 t
ORIGIN      
        1 ggacctgtgg accgcgtgga cgttcgccga cctggtccca gaggaactcc gggccactct
       61 ggaggtgggt cctccggccg gttgatgccg gaggagcgac caacgtcaac gtcgttcgca
      121 tgtctgacgg agcgggatcg agcgacgccg tgtcttgaca ggcgtcgaag gggctcgacg
      181 ccggttcacc acatccacgc cggcctggtg gccggcgcga cgacctccta aacgaatccc
      241 aagcaccggg catccatggg cggtgcagac cgggtgacgg gactgctgga tcccatggcg
      301 atgcatccgg ggttccacga ccgtcgaggt tcccgttcct cgaggctcgg aaggagagga
      361 gggtccatgc gcaggaccat cgggctatcg atcgcaggtg cgctcctgat gatcgcaggc
      421 ttcgtcggac ccgtcgaagc tgccggcgtt cagcggtcca cgacgggcga cgggcgtcag
      481 ctcacgggtg cgggcgtcgg cttccgcacg ttcgcgttcg ctgtcgtcga acacaacgac
      541 ggcagggtca ccggagaagc gagcgtgatg aatccggtga tcggctcgga tcgcgtgttc
      601 gagatctcct gtctgaccgt gatcatcggg aacgacggct cgccgatagc gatcgcgagt
      661 ggacccgtcg tcgccgccga ggatccttcg ttgatcggtc gcaacgggat cttcgccgtc
      721 caggatctcg gcgagggaca tgacccctct gaccggatga ccctcggcgt cctcctgagc
      781 cccgtccggt gcgcggacgt cacttcagag gaggcactcc ggagcgtgtt cccgcgtggt
      841 ctcctcgcca tcgacgatgg gaacatccag gtgcgagcgt agtcggggct cgcgaggccc
      901 cgatgtcctg tctgtaggtg ctcgggtcgt gctcgcgtct cgagcgcatc gacacaagct
      961 cgccggaccg agagatggtc atcggccgcg gcataggctc ctcccatgga gtcgcctgga
     1021 cgagttcggg gcgtgctaaa cgaacccgca gcgccgggca tccatgcggc cgtgcagacc
     1081 gatgacaacg tcgtggatct ccacggggag cgcgagacgt ccgcgacccc gttgagcttc
     1141 gacgacctgt tcctcgatca tcacgatcgc ctgtaccggg ctctctactt catcaccggg
     1201 agctccgccg atgccgagga gctgatgcag gacgcgttcc tgaagctctg ggagcgctgg
     1261 gacaggatcc acacgatcga cgacccagtg gcctacctgt tccggatcgc gatgaacggc
     1321 ttccggatgc gcgctcgacg ggctcgtgtc gccgcgcggc ggatcgtgca cctggagccg
     1381 acacgcgacc cgtacgacga catcgagcta gaagatgacg tccgccgcat gctgctgggc
     1441 ctcccgccca ggcagcgcgc ggcgctcgtg ctcacggaga tcttcggata cagctcggag
     1501 caggccgccc ggatcctcgg gatccgagcg acgaccgtgc gcgtgctcgc ctcgcaaggg
     1561 cgagcgaccc tcagggcgag atgaggagag ctggatgcca gacgtgaaag aactctacga
     1621 gctggtcacg aagcagaggc cgccggacac cggcgcgttc gagcgtcaac ggacccggca
     1681 gatccggacg acgcggaacc gcaaggtcgg cgcgttcgcc gttgtggcgg cgatcggtgt
     1741 cgcggcagcg gccgtgatcc tggggacacc acgaagctcc gaaccgaagc gggaggtcgg
     1801 cggtcgtcca ccggtgagtc cggtggaggt cgccacgagc ttcgtcgagg cctacggcgc
     1861 cctggacgtc gacacggtga tcggcctcct gggcgacgac gccgacatct cgcctttgat
     1921 cgactgggtg aacctccgga ccggagggcc gctggaggaa ctccgcatga cgttctccct
     1981 gctcgaagcc cagggctatt cgcacacgcg cgactcctgt gaggaagtga gccgctcggc
     2041 ttccaacacg agtcttcgct gcacgttcga gctccattgg ctccactcag acgagatcgg
     2101 gcgaggtccg ttcgcgggga gctacttcga tatcaccgtt cgggacggag agatcacacg
     2161 cgtatcgaac tacgtggaga tcgaggagtt ctcacctcag atgtgggaac cgttcgccgc
     2221 ctgggtgtca acgaatcacc cgagagacgt ccttgacatg tacgaggacg agacgcagtc
     2281 cttggagcgg gtcaccgagg agtcgatccg cctctgggat cggtacacac gcgactacgt
     2341 agaggaagtg aaccaagggg tcatcggata gtggcacggg aggcctctcc ccagccacga
     2401 gcgagggagg cacgatggag cgcatcactc gagagccgcg gacggccggg tgcctgtcgt
     2461 cgcccttggc cacatcgagc cgggtggcaa ggatcctgat catctcggtc atcctgactg
     2521 caggcttgcc cgcgtcagcc ggggtcgaag cgggattggc tgaccccgcg ggggatgccg
     2581 aacacggcgc accggaattc atggacatcg tcggtgccga ggcctcgaag agcggacagg
     2641 tgtttcgctt ccagatgagc ctggccgcgc cgatccctgc agttccccct ccgacaccgc
     2701 caggaacgaa ccagatcgag tgggattggt ccctcaacac cgatccgacg accttcccgg
     2761 caggttcccc cttcccggga ggaccgggcc agggaaggcc tgctgagttc atcatccacg
     2821 tcgtgtggga tgggaggtcc ttctcggctc acctgaccga ccgcagacca ctgctgagcg
     2881 gtggggaggc attcatcacg ccgcttgtct tcacgagctc gggcacgacc acgtgggttg
     2941 acctgagcgc gagcgtgctc gacaagccgt cgactttcct ctggggggcg gtgacgttct
     3001 attggtccag cccgccgggc gggaccgcag gcggtcactt cgtggattcc cttgagccgt
     3061 tctacaaccc attcccggca tagcggcgag gtatcagacg cgatctgggt ggcggccggc
     3121 ctagggttgg agatctctca cctctgacat ccgtcggcgg cgatctcgag gttggtctcg
     3181 aagagcatga gttgctgccg aggagcccgg tctccgacgg accgggcttc ggccttgccg
     3241 ccgcgctccg acgcgcggcg cgtgacgcgg gttcgaccta ctcggtcgag cccgcgagct
     3301 ccacaggcgg cgtgtcgggc ggcatgatgc cggcgccgtg ctcgaacgtg atctcaccgt
     3361 catgcgcgtc cacgatgatc gtctcccccg cacggaactc cttgtagagg agcttctcgg
     3421 cgagcggatc ctccaccagc tgctggatcg tgcggcggag cggtcgggcg ccgagggcct
     3481 tgtcgtaccc gcgctcggcg agcagcgtct tggcggcatc ggtgaggacg atgtccacgt
     3541 ccttggactt cagctgctcc tccacccggc gcatcagcag atcgacgatc tgcttgacgt
     3601 catcgggcgt gagggagtgg aagacgatga cctcgtcgat gcggttcagg aactccggcc
     3661 ggaaggagcg cttgagctcc tcggtcacgt tctccttcat cttcgtgtac gtaaccgcgt
     3721 cgtcgcgcgc cgccgtgaac ccgatcccca cgcccttggc gatgtcccgg gtgccgaggt
     3781 tcgaggtcat gatgatgatc gtgttcttga agtccacctt gtggccctgt gcatcggtga
     3841 ggtgtccgtc ctccaggatc tgcagcaagg tgttgaacac gtccgggtgc gccttctcga
     3901 tctcgtcgaa cagcaccacg gcgaacggct tgcggcgaac ggcctcggtg agctgtccgc
     3961 cctcctcgta gccgacgtat ccggggggcg agccgatcag gcgggagacc gtgtgcttct
     4021 ccatgtactc ggacatgtcg agctggatca gcgcatcctc gtccccgaag aggaactcgg
     4081 ccagaacctt ggagagctcg gtcttcccga cgcccgaggg cccgaggaag atgaacgagc
     4141 ccgagggacg gcgggggtcc ttcagcccgc tccgcgtccg gcgcatcgcg cgggagaccg
     4201 cggagatcga ttcgtgctgg ctgatgatcc gcttgtggat ctcctcctcc atgcggagca
     4261 gcttctcggt ctcttcctcg gtgagcttga agacggggat cccggtccag acggagagca
     4321 cctcggcgat ctcctcctcg ccgatctccg ataggacatc catctcgccg gccttccacc
     4381 gctgctcgcg acgctggcgc tcctcctgga gctgccgttc ctcgtcgcgg agcgatgccg
     4441 caccctcgaa gtcctgcgcg tcgatcgccg attccttccg cagacggacc tcggcgatct
     4501 tctcgtcgac ctcacggacg tcgggcggtg ccgtcatccg gcggatccgc gtgcgcgacc
     4561 cagcttcgtc gatcaggtcg atcgccttgt ccggcaggga acggtccgcg atgtagcggt
     4621 ccgcgaggtt ggctgccgcc acgagcgcgt cgtcggtgaa gctcacccgg tggtgggcct
     4681 cgtagcgatc ccggagacct ttcaggatct cgatggtgtg ggagacggtg ggctcctcga
     4741 ccacgatcgg ctggaacctc cgctcgagcg ccgcgtcctt ctcgaggtgc ttgcggtact
     4801 cgtcgcgtgt ggtggcgccg atcgtctgca gctcaccgcg cgcgagcatc ggcttgagga
     4861 tgctggcggc gtcgatcgcg ccttccgcgg cacccgcgcc caccagcgtg tgcagctcgt
     4921 cgatgaacag gacgatgtcg cctcgggtct ttatctcctt gaggaccttc ttcaggcgct
     4981 cctcgaagtc gccccggtac cgggagccgg cgacgagcgc gcccaggtcg agcgtgtaga
     5041 tctgcttgcc cttcagcgtc tcgggcacct cttcacggac gatgtcttgc gacaggccct
     5101 cgacgacggc ggtcttgccg acccctggct cgccgatcaa caccgggttg ttcttggtcc
     5161 ggcgcgacag gacctgcatg acccgctcga tctccttctc acggccgatc acggggtcga
     5221 gcttgccgtc gcgggccagc tgggtgaggt tccgaccgaa ctgatcgagg accatcgacc
     5281 cctggccgcc gccctcctgg gaagccccca cctgctcgcc cttgccggcc gtgtagccgg
     5341 acaggagctg gatgaccgtc tgacggacac ggttcaggtc ggcgccgagc ttctggagca
     5401 cctgcgcggc gaccccttcg ccttcgcgga tcagcccgag caggatgtgc tcggtgccga
     5461 tgtagttgtg tccgagctgg agcgcttcgc ggagcgagag ctcgaggacc ttcttggcgc
     5521 gcggggtgaa cgggatgtgg ccggtgggcg cggcttgccc ttgcccgatg atctcctcga
     5581 cctgttggcg aacggcttcg agagagatgt tcaacgactc gagcgccttg gccgcgacgc
     5641 cttcgccttc gtggatcaga ccgagcagga tgtgctcggt gccgatgtag ttgtggttga
     5701 gcatcctcgc ttcttcttgg gcgaggacga ccacccgccg tgctcggtcg gtgaatcgct
     5761 cgaacatgcg ctacatccct ccaggcggcg acccgtccgt ccggccccgg ggatcttcgg
     5821 actccgtcct acccccgagg tgcatgaggc gccgctccct cgcggtggca gcgcctctct
     5881 gcccgcagta tacccagcgc ccccgatacg ccagcacccc gcccaacttc cgctccgttc
     5941 actcagggag gatctcgtcc agagcaactc tcatctcagt cttcggaaga accgagcccg
     6001 gggatgagtc cggacgacct ttccgagggg tgagacgcgg catagacctt tcggaccaga
     6061 aggaccaccc ggaaggccgt tggcatcccg gggctcgggc tgtcagcatc gtggcaacgc
     6121 tcctcgtgtc ccccacatgg gagcctcccc agcgttcgga ggtccggcct tccccatgtc
     6181 gacaccatgg agaaagagag cgagacagtg cgcgtgctcg tcgtcgatga tcatcccgcg
     6241 ttccggaagg ccctcgcatc ggccctgcaa ctcgtcggcg atatcgagat cgcgggggag
     6301 gcctcgggcg gcgaggacgc ctgcgtcggc gcgaaggagc tgcagcctga cgtcgtcctg
     6361 atggatctct cgatgcccga catctccggc atcgaggcga tgcagcggat ccattcgtcg
     6421 accccggagc tccccgtcgt gatcctcacc gcccatgcgg acgagggcgt cgagcgagcg
     6481 gcgcgggggg ccggcgcctc ggggttcgtc gcgaagggac gagggctcca ggacatcctc
     6541 cgagcgctgc acgaggtgac ggccgcgcgc tgagcctcgc gatagggtcc cccgcgtggg
     6601 atgcctcatc gtcctgttcg tccttctcct gccccgcacg atcatggtcg ccctgtggct
     6661 gttctccagc tatctctcga ccgcgttcgg gacctgggtc tggccgttga tcggcttctt
     6721 cgtgctcccg acgaccacgc tcgcctacgc ggtggcccag aaccgctacg gcggcgtgcg
     6781 tggcctcggg ctcgtgctgg tgatcctcgg tgtcctggtt gacctcggcg cgctcggagg
     6841 cggaggcagg ggcgccggga agcgctgggc cggagatcgc tgagggccga acggaggggg
     6901 ccggctcgcc ttcggcgagc cggccctcac ggatgccccg ggtgcgtcgg ggccaccccc
     6961 tgtttcccca gccgcgtcgc gtcgatcgct cggcgctgcg cttccgggcc atccgggtcg
     7021 ttcgagaccc acccgaacgt atccacggag ctccgcgagg gatgtaacgc acgtgacaac
     7081 cgcggggtgc tgttcgtcac cggccgaagg tgacgtaggt cacgtccgga tcagtcgagc
     7141 gcctctgggc ggtgggcggg gaacatgatg aggtcgcgga gcgatgcggt gtccgtcagg
     7201 accatgagca ggcggtcgat gccgagaccc agccctccgg ccggcggcat cccgtgctcg
     7261 agcgcccgga ggaagtcctc atcgagcgga tgcgtctcct cgacgcccgc cgccttcagc
     7321 tgctgttgga gctcgagctt cgcacgctgc tcgagcgggt cggtcagctc ggagaacgcc
     7381 gtgacgagct cgaccccgcc gatgatcagg tcgaa
//
LOCUS       DASUYD010000181         6334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_108186, whole genome shotgun sequence.
ACCESSION   DASUYD010000181 DASUYD010000000
VERSION     DASUYD010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108186"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /locus_tag="VF195_10235"
     CDS             complement(<1..791)
                     /locus_tag="VF195_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6581235.1"
                     /translation="MAGRILSTSHGTHRGSFAAVDWVLFAFIGTIWGSSFLFMAIGLE
                     AFRPGLVTWLRILFGAALLWTLPGVRTRIDRADLVPLLILAVSWVAIPFTLFPLAQGS
                     VNSAIAGMLNGAAPIFTVVVATILFRRAPGSRQLLGIPIGFLGVAAIALSAAGEGRSQ
                     EVGVVLILIATVGYGVAFNVATPLQQKYGALPVMARILAIGAVLTAPIGLTSVPGSRF
                     AWSALLAVAAVGMLGTGVAFVLMGRLVGRVGATRASIVGYLIPVVA"
     gene            complement(812..1699)
                     /gene="deoC"
                     /locus_tag="VF195_10240"
     CDS             complement(812..1699)
                     /gene="deoC"
                     /locus_tag="VF195_10240"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX6581236.1"
                     /translation="MAVRTTAVDAVGLEERAASLAKRSIKRDAKLWALDLAIRSMDLT
                     TLEGSDTVGKIVAICAKAIRPNPLDPSIPPVAAVCLYPQLVPVALEQLHGTNVKVASV
                     AGGFPSGLGPLDARLQEIRDVAAAGAHEIDIVLNRSLFLGGRYAQAFEELVAAREAAG
                     PAHLKVILETGELGSYDRVRQASMLAMAAGADFIKTSTGKIGTNATLPVALCMLEAAR
                     DFHRETGRAIGIKVAGGVRTGKQAIQYLVLVHETLGTDWMTPDRFRIGASSLLNDVLM
                     QIDKERTGRYAGPDYFTID"
     gene            1804..2169
                     /locus_tag="VF195_10245"
     CDS             1804..2169
                     /locus_tag="VF195_10245"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6581237.1"
                     /translation="MGTLPDMRIARVLLVALAAMVPACEGETAPPEPCTDPAPATSVQ
                     LADFAFRPDCLSADPAATIALDNTGDAPHTFTVEGTDVDVNVDAGSSGEADLGTVGPG
                     TYPVTCTFHPQMEATLTVG"
     gene            complement(2173..3036)
                     /gene="era"
                     /locus_tag="VF195_10250"
     CDS             complement(2173..3036)
                     /gene="era"
                     /locus_tag="VF195_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013117428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX6581238.1"
                     /translation="MVAVVGRPNVGKSTLVNALVGRKVSIVSDKPQTTRRGLHAVLTT
                     DGAQAVFTDTPGFHKPRTELGARLNDMVGDAVEGVDVILHVLDARSGIGHGDAFVYEH
                     RVRPHACPKIAVVNKIDVMGQHGVVPQLAAAAEIGDYDEIVPVSAKAGKGVGMLRDMV
                     LAMLPEGPALYPEETISDQPLETRLAEMVREKALHVTRQELPHSITVTIEELDITDAL
                     VKVHASLIVERDSQKGIVIGKGGRTLRTIGTRAREEMELVLGTKVFLDLRVKVVKEWQ
                     RDPKALERLGF"
     gene            complement(3063..3476)
                     /locus_tag="VF195_10255"
     CDS             complement(3063..3476)
                     /locus_tag="VF195_10255"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEX6581239.1"
                     /translation="MTSIGPDRIDELVAAAREIRAAAYAPYSGFHVGAALLAGERIFT
                     AVNMENASYPLSVCAERNAVAMAVAAGETSLRVVAVVTDAETKPTPPCGGCRQVLWEF
                     GEGMAVIAESASGAERRYWALADLLPDAFGSGDLR"
     gene            complement(3473..4771)
                     /locus_tag="VF195_10260"
     CDS             complement(3473..4771)
                     /locus_tag="VF195_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX6581240.1"
                     /translation="MSWLWLVALLLVLFGTVLAAAEASLTRTSRIRALALEEDGRRNA
                     ETLVKIESDPPRYLNAVYLTVMFAQNGSAILVAILAENDFGPEDGSGPWVGVVSFVFT
                     ILYFVVVEAMAKTFAVLHTDRVALTLAPVVWFLGRVLELPTRFLIGLANVLLPGKGLK
                     QGPFVSEEEIRSMAEVGSEEGSIDQGEKELIHSIFEFGDTIAREVMVPRPDIVAIEDD
                     KTLRDVQALVLQHGYSRIPVYHESLDDDDDGRGVKGVVFAKDVLKALHQGRQDIPLSE
                     IVRPARYVPESKKVADLLKEMQQEKFHQALVYDEHGSVTGIVSLEDLLEELVGEIADE
                     YDREEPEVLDLGDGMYRVSGKASIDDVNELLDAELPDEEWDTVAGLMLDLLGRMPEAG
                     EEVTFRGLQLKADKVQGRRIASVLITRSLEENGDKPEVAT"
     gene            complement(4768..5268)
                     /gene="ybeY"
                     /locus_tag="VF195_10265"
     CDS             complement(4768..5268)
                     /gene="ybeY"
                     /locus_tag="VF195_10265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX6581241.1"
                     /translation="MADEDHLADPEPATRPGDGWPHVLISNRQHDPIDEEGMRSLARE
                     TLLAEGIEHAELSVSFVDADEIAELHERFMDEPGPTDVLSFPLDDVDGAGGRAEGREG
                     VSFLGDVVVAPTEAVRNNPHDPAAELRLLLVHGILHLLGYDHEDDDERTRMWERQERY
                     SGVQAP"
     gene            complement(5268..6227)
                     /locus_tag="VF195_10270"
     CDS             complement(5268..6227)
                     /locus_tag="VF195_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEX6581242.1"
                     /translation="MVALLGQQDAFLKLIEAGFSSDILVRGNEITITGPEHEAEQLAH
                     LFEELLALLEKGHQLSDSSVGQTIELIKGGDEWAEGDDGTVRMRPSEVLGDTLLTARG
                     TSVAPKTVGQKRFVDAMRRATVTFAIGPAGTGKTYLAVATAVRALQDHQVSRLILTRP
                     AVEAGERLGFLPGTLYEKIDPYMRPLYDALFEMTGAESLSRLMERGTVEVAPLAYMRG
                     RTLNDAFIILDEAQNTTPEQMKMFLTRIGFGSKAVVNGDDTQVDLATGQRSGLLVIEE
                     ILSGIRGVSFCRLGGRDVVRHKIVQEIVEAYDRYGQQRLGMDD"
BASE COUNT          991 a         2249 c         2107 g          987 t
ORIGIN      
        1 gccaccacgg ggatcaggta gccgacgatc gaagcgcgcg tcgctcccac ccgccccacg
       61 agccgaccca tcagcacgaa cgccacgccg gtcccgagca tgccgacggc ggccaccgcg
      121 aggagcgcgg accatgcgaa ccgggagccc gggaccgagg tcagcccgat cggcgccgtg
      181 aggacggcgc cgatcgcgag gatccgcgcc atgacgggga gcgccccgta tttctgctgg
      241 agcggggtcg ccacgttgaa ggcgaccccg tagccgaccg tcgcgatcag gatcagtacg
      301 acgccgacct cctggctccg gccttcacct gcggcggaga gcgcgatcgc ggcgacgccg
      361 aggaaaccga tcgggatccc gaggagctgg cgtgaccccg gtgcccggcg gaacaggatc
      421 gtggcgacga ccaccgtgaa gatcggcgcg gcgccgttca gcatcccggc gatcgccgag
      481 ttgaccgagc cctgggcaag ggggaagagc gtgaacggga tcgcgaccca cgacacggcg
      541 aggatcagta gcggaacgag gtcggcgcgg tcgatccgtg tccggacccc gggcagcgtc
      601 cacagcaggg ccgcgccgaa caggatgcgg agccacgtaa ccagcccggg ccggaaggcc
      661 tccagcccga tcgccatgaa caggaaggac gagccccaga tcgtgccgat gaaggcgaac
      721 agcacccaat cgacggccgc gaacgagcct cgatgggtgc cgtgcgaggt cgacaggatc
      781 cgcccagcca tcgcctgaac aacgaccaac gtcagtcgat cgtgaaatag tccggacccg
      841 cgtagcggcc cgtgcgctcc ttgtcgatct gcatcagcac gtcgttcagg agggacgacg
      901 cgccgatccg gaaccgatcc ggcgtcatcc agtccgtccc aagcgtctcg tgcacgagca
      961 cgaggtactg gatcgcctgc ttcccggtcc gcacgccacc ggcaactttg atgccgatcg
     1021 cccggcccgt ctctcgatgg aagtcccggg ccgcctccag catgcagagc gcgaccggga
     1081 gcgtggcatt cgtgccgatc ttgcccgtgg acgtcttgat gaagtcggct ccggcagcca
     1141 tcgcgagcat gctcgcctgg cggacccggt cgtaggagcc gagctcaccg gtctcgagga
     1201 tcaccttgag gtgcgccggc ccggcggcct cccgagcggc gaccagctcc tcgaacgcct
     1261 gtgcgtagcg ccccccgagg aagagcgagc ggttcagcac gatgtcgatc tcgtgggcgc
     1321 ccgccgccgc gacgtcccgg atctcctgca gccgtgcgtc gagcgggccg aggccgctcg
     1381 ggaagccgcc ggccacgctc gcgaccttca cgttggtgcc gtggagctgc tccagtgcca
     1441 ccggcacgag ctgcgggtag aggcatacgg ccgcgaccgg cgggatcgag gggtcgagcg
     1501 ggttcggccg gatcgccttc gcgcagatcg ccacgatctt gccgaccgtg tcgctcccct
     1561 ccagtgtcgt gaggtccatc gaccggatcg cgaggtcgag cgcccagagc ttggcgtcgc
     1621 gcttgatcga tcgcttcgcg agcgacgccg cgcgctcctc gagcccgacc gcgtcgaccg
     1681 cggtcgtccg caccgccatc cgcacggcct caccggaggc gaccggcaac ggccggctcg
     1741 gctcgatctg cgctgcttgg gccatggctc gatggtagcc cacccggtcg tcacgaggtc
     1801 cgggtgggta ccctccccga catgaggatc gcccgtgtgc tcctggtcgc cctcgccgcg
     1861 atggtccccg cctgcgaagg cgagaccgct ccgccggagc cgtgcacgga tccggccccg
     1921 gcaacctcgg tgcagctggc ggacttcgcg ttccgcccgg attgcctgtc ggccgacccc
     1981 gcggcgacga tcgcgctcga caacaccggc gatgcgccac acacgttcac ggtggagggc
     2041 acggacgtcg acgtgaacgt cgacgccgga tcctcgggcg aggcagacct cgggaccgtg
     2101 ggtccgggga cctatccagt gacgtgcacg ttccatccgc agatggaggc aaccctcacc
     2161 gtcgggtgac ggtcagaacc cgagtcgctc gagcgccttc gggtcgcgct gccactcctt
     2221 cacgaccttc acgcggaggt cgaggaacac cttcgtcccg aggaccagct ccatctcctc
     2281 gcgggcccgc gtgccgatcg tccggagcgt ccgccccccc ttgccgatca cgatcccctt
     2341 ctgggaatcg cgttcgacga tcagcgatgc atggaccttc acgagcgcgt ccgtgatgtc
     2401 gagctcctcg atcgtgaccg tgatcgagtg cgggagctct tggcgggtca cgtggagcgc
     2461 cttctcgcgg accatctccg cgaggcgggt ctcgagcggc tgatccgaga tcgtctcctc
     2521 cggatagagc gccggacctt ccgggagcat cgccagaacc atgtcccgga gcatgccgac
     2581 acccttcccg gccttggcgg acaccgggac gatctcgtcg tagtcgccga tctccgcggc
     2641 cgcggcgagc tggggcacga cgccgtgctg acccatgaca tcgatcttgt tcaccaccgc
     2701 gatcttgggg cacgcgtggg ggcgaacccg gtgctcgtag acgaacgcgt cgccgtggcc
     2761 gatcccggac ctcgcgtcga ggacgtgcag gatcacgtcc accccctcga ccgcgtcgcc
     2821 gaccatgtcg ttgagtcggg cacccagctc cgttcgcggc ttgtggaacc cgggggtgtc
     2881 ggtgaaaacg gcctgcgcgc catccgtcgt caggaccgca tggagcccgc gacgtgtcgt
     2941 ctgcggcttg tcggacacga tcgacacctt gcgtccgacg agcgcgttca cgagcgtgga
     3001 cttgccgaca ttgggtcggc ccacgacggc gaccatgccg gaacgcgtaa ccgcgaggtc
     3061 gatcaccgga ggtcgcccga accgaaggcg tcgggcagca ggtcggccaa ggcccagtac
     3121 cggcgctcgg cgcccgatgc cgactcggcg atcacggcca tcccttcccc gaactcccac
     3181 agcacctgac gacacccccc gcatggcggg gtgggcttcg tttcggcgtc cgtgacgacg
     3241 gcgaccaccc gcagcgatgt ctcgcccgct gcaacggcca tggcgactgc gttgcgttcc
     3301 gcgcacaccg acagcgggta gctggcgttc tccatgttca cggccgtgaa gatgcgttcg
     3361 ccggccagca gagccgcgcc cacgtggaag ccgctatacg gagcgtacgc cgcggcccgg
     3421 atctcgcgtg cggcggcgac caactcgtcg atgcggtccg gcccgatcga ggtcacgtcg
     3481 ccacctccgg cttgtcgccg ttctcctcga gcgagcgcgt gatcagcacg ctcgcgatcc
     3541 ggcgtccctg gaccttgtcc gccttgagct gcaggccacg gaacgtgacc tcctcaccgg
     3601 cctccggcat ccggccgagc aggtcgagca tcagaccggc gacggtgtcc cactcctcgt
     3661 ccggcagctc ggcgtccagg agctcgttca cgtcgtcgat cgaggccttc ccggagaccc
     3721 ggtacatccc gtcgccgagg tccagcacct ctggctcctc gcggtcgtac tcgtcggcga
     3781 tctccccgac cagctcctcg aggagatcct cgagtgacac gatcccggtc accgagccgt
     3841 gctcgtcgta cacgagcgcc tggtggaact tctcctgctg catctccttc aggaggtccg
     3901 cgaccttctt cgattccggg acgtagcgcg cggggcggac gatctccgag agcgggatgt
     3961 cctgccggcc ctggtgcagg gccttcagca cgtccttcgc gaacacgacg cccttgacac
     4021 ccctcccgtc gtcatcgtcg tccagggact cgtgatacac ggggatccgg gagtagccgt
     4081 gctgcaggac gagcgcctgc acgtcgcgga gcgtcttgtc gtcctcgatc gccacgatgt
     4141 cgggacgcgg caccatcacc tcgcgcgcga tcgtgtcgcc gaactcgaag atcgagtgga
     4201 tcagttcctt ctcgccctgg tcgatcgagc cctcctccga tcccacctcc gccatcgatc
     4261 ggatctcctc ctccgagacg aacggcccct gcttgaggcc cttgcccggg agcagcacgt
     4321 tcgccaggcc gatgaggaac cgtgtcggga gctcgaggac gcggccgagg aaccacacga
     4381 ccggggccag cgtgagcgcc acacgatccg tgtgcaggac cgcgaacgtc ttcgccatcg
     4441 cctcgacgac gacgaagtac aggatcgtga acacgaacga gacgacaccg acccaggggc
     4501 cgctgccgtc ctcggggccg aagtcgttct ccgcgaggat cgcgacgagg atcgccgagc
     4561 cgttctgcgc gaacatcacc gtgaggtaga ccgcgttcag gtagcgcggc ggatcggact
     4621 cgatcttcac gagcgtctcc gcgttgcgcc gaccgtcttc ctcgagggcg agcgcgcgga
     4681 tccgtgacgt ccgtgtgagc gacgcctcgg cggccgcgag gacggtcccg aacagcacca
     4741 gcaggagcgc gacgagccag agccagctca cggagcctgc accccgctgt accgctcctg
     4801 ccgctcccac atccgtgtgc gctcgtcgtc gtcctcgtgg tcgtagccga ggagatgcag
     4861 gatgccgtgg acgagcagga gccgaagctc ggccgcgggg tcgtgaggat tgttccgaac
     4921 ggcctcggtg ggagcgacga cgacatcccc gaggaagctg acgccctctc gtccctcggc
     4981 tcgtcccccg gctccatcga cgtcgtcgag cgggaacgac aacacgtcgg tcgggccggg
     5041 ctcgtccatg aatcgctcgt gcagctcggc gatctcgtcg gcatcgacga acgagacgga
     5101 cagctccgca tgctcgatgc cttccgcgag gagggtctcg cgggcgagcg accgcatgcc
     5161 ctcctcatcg atggggtcgt gctgccggtt cgagatcaac acgtgcggcc atccgtcgcc
     5221 ggggcgagtc gccggctcgg ggtcggcgag atggtcctcg tcggccatca gtcgtccatc
     5281 ccgaggcgct gctgaccgta gcggtcgtat gcctcgacga tctcctgaac gatcttgtgc
     5341 cgcacgacgt cgcgcccgcc gagccggcag aacgagacac cgcggatccc cgagaggatc
     5401 tcctcgatca cgaggagacc ggagcgctgc ccggtggcga ggtccacctg agtgtcgtcg
     5461 ccgttcacga cggccttcga tccgaacccg atccgcgtga ggaacatctt catctgctcc
     5521 ggcgtggtgt tctgggcctc gtcgaggatg atgaacgcat cgttcaaggt cctgccgcgc
     5581 atgtaggcga gtggcgcgac ctcgacggtg ccccgttcca tcagccgcga gaggctctcg
     5641 gcgccggtca tctcgaacag cgcgtcgtag agcggacgca tgtacggatc gatcttctcg
     5701 tagagcgtcc ccggcaggaa cccgagccgc tctcccgcct ccacggcggg acgggtgagg
     5761 atcagccgcg agacctggtg atcctggagc gcgcgcaccg cggtggcgac ggccaggtag
     5821 gtcttgcccg tgccggcggg tccgatcgcg aacgtcacgg tcgcgcgccg catcgcgtcg
     5881 acgaaccgct tctgcccgac cgtcttgggc gcgaccgagg tcccccgcgc ggtgaggagc
     5941 gtgtcgccca gcacctcgct ggggcgcatc ctgacggtcc cgtcgtcgcc ctccgcccac
     6001 tcgtcgccgc ccttgatcag ctcgatcgtc tggccgacgg aggagtcgga gagctgatga
     6061 cccttctcga gcaacgcgag gagctcttcg aagagatgcg cgagctgttc ggcctcgtgc
     6121 tcggggccgg tgatcgtgat ctcgttgcct cggacgagga tgtcggacga gaacccggct
     6181 tcgatcaact tcaggaacgc gtcctgctgg cccaggagcg ccaccatcgg ctggtggccg
     6241 gggacgagga tcttcacttg ggtggtggcg gctgccacgt gtcgcgctct cgggggtgac
     6301 cccgttcctc ctgttgtcga gagcgggacc cgtc
//
LOCUS       DASUYD010000182         3962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_550471, whole genome shotgun sequence.
ACCESSION   DASUYD010000182 DASUYD010000000
VERSION     DASUYD010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550471"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF195_10275"
     CDS             complement(<1..189)
                     /locus_tag="VF195_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581243.1"
                     /translation="MGRPITHFEIASPNLESATEFYRQLFDWSVGEELIDGYRLVQTA
                     EGSIGGGLLRTPEGVFPYV"
     gene            complement(974..2602)
                     /locus_tag="VF195_10280"
     CDS             complement(974..2602)
                     /locus_tag="VF195_10280"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6581244.1"
                     /translation="MRSSDPGAGLRWTLPVFTSLAGYRRAWLARDLVAGVLVVAIAIP
                     LSMGMAEVAGMPPIAGLYSCVLPLLAYALFGSSPQLVIALDASTAAMVAAAVAPLAAG
                     DQIRYAELAGAAALIVGGILIVAGPLRVGFVANLLSRPVLLGYQAGLAFVVMFSQLPK
                     LLGLEVVEDRPLPRAIEIVRSLGDASSQVVVIGMACLGVVLMFAAKKSHIPGALFAIV
                     GATFLVELFGSALGDVPVVGSLPAGLPPIRLPRVSTGDIVALLPASAAIAFIAAADTI
                     VSSTAFAHRNHYEVDANRDLVGLGAANLASGVSGGITTSASAARTAVVEMVGGRSQIA
                     SVTAALVMVTVLLFLTRPLENVPTAALAAVVVGAVVRLIEIRSLRALWRIRRTEFAIA
                     MATIVGAVAIGLLEGIIIGVSLSVLDHLVRVALLRRGGERPGRTGPVLIATAPTANEI
                     LVLVDGLLFFANAHQLKDDVRARVGEQPAATRVVVDASRLTDADATAAEMLAQLGDDL
                     ATHGVMLELTELRPPVRDVLKEGAVRGLRETMSV"
     gene            complement(2796..>3962)
                     /locus_tag="VF195_10285"
     CDS             complement(2796..>3962)
                     /locus_tag="VF195_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6581245.1"
                     /translation="TEVEMEFLGLVGMADPVRPEVPDAVAIARRAGIRIVMITGDHPA
                     TAMSVARDAGIPAHTVMIGSELPVSDAALGALLSTHELVLARIAPEQKLRIARALQER
                     GEVVAMTGDGVNDAPALRRSDIGIAMGKTGTDVAREAADLVLLDDNFAHIVEAVREGR
                     AAFDNIKRFLTYHLTDNVAELTPFAVWALSAGAIPLMISVLQVLALDIGTDLLPALAL
                     GAEKAEPGIMQRSPRSPNARLLDRGVLARAFGFLGPVEAVLAMAMLPIGAALFFGWPG
                     EPLPSAGANKQFLSTMVFASIVAMQMANAFECRSNPASLFAIGPLANRLLLGAVAIEA
                     LTLLAFVYVPPIRDLLGQQPLSALAWIPILVAPCVFMAAEETRKAIVRARPRRR"
BASE COUNT          704 a         1396 c         1317 g          545 t
ORIGIN      
        1 cacgtacggg aagacgccct caggcgttcg cagcaggccg cccccgatgc tcccctcggc
       61 cgtttggacc agccggtagc catcgatcaa ctcctcgccc acactccagt cgaagagctg
      121 ccgatagaac tcggttgccg actcgaggtt cggtgatgcg atctcgaagt gcgtgatcgg
      181 ccgtcccatc gtgggccctc ctcacttgat cacggtgtaa tgctctcgcc ccgtcgacct
      241 gtggcagagg gtcgaacgtc ccctgatccg cgcgaccttc acagctcatc gcgctgccac
      301 cccgtcggtg aggccgtcag gatcctcggc cgtgggcgtg cccacgacga cggacacgcc
      361 cgtcgactcg tcggtgcggg catctaccca ggtgcaccgg acgggcagca acagacgggg
      421 tcgaggccga acacccttga gcgagctcgg tggtcagcct gtgagggact ccgcgatcgg
      481 catgccgatc gccagcaccc ccaggaagag gttcgtggcg aaacccaccc agcgccacgg
      541 aagcccttca ggacggaacg gagaccgaag tatgcaccga cagcgaacgg agccatcagc
      601 agcgacccca ggagcgcagc cttccagagt tcccaatccc cctcgccccc gaggtcgaag
      661 accgcttctg ccagggtgaa accgacgaat ggcgctgccg gcagccacaa caggaagtac
      721 cgcgacagct tggactcgcg ggctcctggt atctgcctgg ttggaggcga agcgctcgcc
      781 gcaccagaac ggacacgaat ggcactcatg acgcttctcc tgtgactctc gctctacgct
      841 ccaacgctaa atgcggcccg gtccggccga agggtcttag gtcccgcttc ggctgggccc
      901 cacccctgcg tctatcgatc ccggggacca cagttgaagc tcgaacgttc cgaaacgtcc
      961 agccgtctgc acctcagacg ctcatcgtct cgcgcaaccc tcggacggcg ccttccttca
     1021 gcacatctcg caccggtggc cgcagctccg tgagctcgag catcactccg tgggtggcga
     1081 gatcgtcccc cagctgggcg agcatctccg ccgcggtcgc gtccgcatcc gtgagccgcg
     1141 aagcgtcgac gaccactcgt gttgcggccg gctgttcgcc cacgcgagct cgaacatcat
     1201 ccttgagctg gtgagcgttc gcgaagaaca gcaacccgtc gacgaggacg aggatctcgt
     1261 tggcggtcgg ggcggtcgcg atcagtacgg gcccggtcct gcccgggcgc tcgccacccc
     1321 gacggagcag ggcaacacgc acgaggtgat ccagtacgga caggctcacg ccgatgatga
     1381 tcccttcgag gaggccgatg gccacggcgc cgacgatggt cgccatggcg atcgcgaact
     1441 cggttcgacg gatccgccag agcgcgcgga gcgaccggat ctcgatcagt cgtaccaccg
     1501 cccccacgac gacggcggcg agcgccgccg tcggaacgtt ctcgaggggc cgggtcagga
     1561 agagcagcac cgtgaccatc accagcgccg ccgtcacgct cgcgatctgt gatctgccac
     1621 cgaccatctc cacgacggcc gtgcgagcgg cgctcgcgct cgtcgtgatg ccgcccgata
     1681 ccccagatgc gaggttcgcc gctcccagtc ccaccagatc gcggttcgcg tcgacctcgt
     1741 aatggttccg gtgcgcgaac gctgtggagc tcacgatcgt gtccgccgct gcgatgaagg
     1801 cgatcgccgc ggatgccgga aggagcgcga cgatgtcgcc ggtggacaca cgcgggaggc
     1861 ggatcggcgg caaccccgcc ggcagacttc cgaccaccgg cacgtccccc agggcggagc
     1921 cgaacaactc gaccaggaag gtcgccccga cgatcgcgaa cagggccccg gggatgtgcg
     1981 acttcttcgc ggcgaacatc aagaccaccc cgaggcaagc catcccgatc acgacgacct
     2041 gcgagctggc gtctcccagg ctgcgcacga tctcgatcgc tcgcggcaac ggtcggtctt
     2101 cgaccacctc gagcccgagg agcttaggga gctggctgaa catcacgacg aaggcgaggc
     2161 cggcctggta gccgagcagc accggccggg acaagaggtt cgccacgaac ccgacccgga
     2221 gcggccccgc tacgatcaag attccgccga cgatgagcgc agcggctccc gcgagctctg
     2281 cgtagcggat ctgatctccc gccgcgagcg gggcgacggc cgccgccacc atcgccgcgg
     2341 tggaggcgtc gagcgcgatc acgagctggg gcgacgatcc gaacagggca taggcgagga
     2401 gcgggagcac gcagctatag agcccggcga tgggcggcat cccagcgacc tccgccatcc
     2461 ccatgctgag cgggatcgcg atcgcgacga cgaggacgcc ggccaccaga tcacgcgcca
     2521 accacgcccg gcgatagccc gccaaggacg tgaagacggg cagcgtccac cgcagccccg
     2581 ctcccgggtc cgaagacctc atcgcctcga gcgtctcgcc gaagcagctg gaagactgga
     2641 gccgttcgtc cgggccgtga tgggaccttc ggcgggaccg cacgacggcg ccggcgctgc
     2701 ggtcgaacct cgccgatctc accgaaccga agcagctggt tcgtggcccc gggagctcgg
     2761 ctgagcctcg gggcacacat gccgcgtcgc gtgagtcagc gccgtcgagg ccgagccctg
     2821 acgatcgcct tccgcgtctc ctcagcggcc atgaacacgc aaggagcgac gaggatgggg
     2881 atccacgcga gcgcgctcag gggttgctgg ccgagcaggt cgcggatcgg cggtacgtag
     2941 acgaacgcga ggagcgtgag cgcttcgatc gcgacggcgc cgagcaagag ccggttggcc
     3001 agcggaccga tcgcgaacag cgacgcgggg ttcgagcgac attcgaacgc gttggccatc
     3061 tgcatcgcga cgatggaggc gaagaccatc gtcgacagaa actgcttgtt cgcgcccgcc
     3121 gatgggagcg gctcccccgg ccagccgaag aacagcgcag cgccgatggg cagcatcgcc
     3181 atcgcgagga ccgcctccac gggtccgagg aagccgaacg cacgcgcgag cacgccccga
     3241 tcgagtagtc gcgcgttcgg cgaccggggc gaacgctgca tgatccccgg ctcggccttc
     3301 tcggcgccga gcgcgagggc cgggagcagg tccgtgccga tatccagggc gagcacctgg
     3361 agcaccgaga tcatcagcgg gatcgcgccg gccgacaggg cccagacggc gaacggcgtc
     3421 agctcggcca cgttgtccgt gaggtggtag gtcaggaacc gcttgatgtt gtcgaacgcc
     3481 gctcggccct cgcggaccgc ctccacgata tgcgcgaagt tgtcgtcgag aagcacgaga
     3541 tccgccgcct cacgagcgac gtcggtcccg gtcttgccca tcgcgatgcc gatgtcggaa
     3601 cgacgcaggg ccggcgcgtc gttgacaccg tcgccggtca tcgcgaccac ctcacctcgc
     3661 tcctgcaggg cgcgcgcgat ccggagcttc tgttcgggcg cgatgcgggc caggacgagc
     3721 tcgtgcgtcg acaggagggc cccgagcgcc gcatctgaga cggggagctc ggatccgatc
     3781 atcaccgtat gtgcggggat gccggcgtca cgggcgaccg acatcgcggt cgccgggtgg
     3841 tcgcccgtga tcatcacgat ccggatcccc gcccgccgag cgatcgcgac cgcatccggg
     3901 acctcggggc ggacggggtc ggccatcccc acgaggccga ggaactccat ctcgacctcg
     3961 gt
//
LOCUS       DASUYD010000183         4024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_769526, whole genome shotgun sequence.
ACCESSION   DASUYD010000183 DASUYD010000000
VERSION     DASUYD010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769526"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..468)
                     /gene="rplI"
                     /locus_tag="VF195_10290"
     CDS             complement(19..468)
                     /gene="rplI"
                     /locus_tag="VF195_10290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX6581246.1"
                     /translation="MKVILQKTVERLGDPGDVADVADGYARNFLIPRGLAVRAEKGTV
                     RHAESLKRAHQSRSKAEKGEFEAIAARIIQTPVVVTARAGEEGKLFGSVTAADIAGAL
                     SAETGIAVDRRDIHLDEPIRSVGTHEVTVHLHPEVDPVITIDVRAQE"
     gene            complement(479..763)
                     /gene="rpsR"
                     /locus_tag="VF195_10295"
     CDS             complement(479..763)
                     /gene="rpsR"
                     /locus_tag="VF195_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011438874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX6581247.1"
                     /translation="MAGRGGPKERGPKRRREKDDKGWQKRGKRKYCQFCKDKVDRVDY
                     KDVMTLRKFVSDRGKIRARRVSGNCVQHQRDVASAVKNAREMALLPYSSR"
     gene            complement(753..1214)
                     /gene="ssb"
                     /locus_tag="VF195_10300"
     CDS             complement(753..1214)
                     /gene="ssb"
                     /locus_tag="VF195_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX6581248.1"
                     /translation="MATDNQVTLVGNLTDDPELRYTPNGAAVCKFRIAVNRRFQDANG
                     QWKDGDTSYFTINAWRTMAENAAESLTRGTRVIVAGRLNYRTWENQDGDKRSAIEVEA
                     DEVAPSLKWATASVTRQARSGGASSSGDWGEKVAAPVGGGTEPEETPLDGG"
     gene            complement(1221..1586)
                     /gene="rpsF"
                     /locus_tag="VF195_10305"
     CDS             complement(1221..1586)
                     /gene="rpsF"
                     /locus_tag="VF195_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX6581249.1"
                     /translation="MREYESMLILPAAADEAVVGTATDRIATVAAKHSGAIRNIERWG
                     RRRLAYEIDDQNEGYYVVVTFSAEPSVQAELERTLNLADEILRYKVIVLPSPRGQKKK
                     QMASSPASAAGGDETPQGE"
     gene            1749..2531
                     /gene="panB"
                     /locus_tag="VF195_10310"
     CDS             1749..2531
                     /gene="panB"
                     /locus_tag="VF195_10310"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX6581250.1"
                     /translation="MTTIHDLKAWKADGRRFTMLTAYDFPTAQILDRAGVPLLLVGDS
                     VGNNVLGYEDTLPVTMAEMLHHTRAVARGAERAMVIGDLPFLSYQVSIEEGVHNAGRL
                     VKEGGAHAVKLEGPHLDLVHRLVDIGIPVMAHIGLTPQSVHAMGGYRVQGRGEAAHRV
                     ADEAEQLEKAGAFSIVLEGMPAELAGDITGSLEIPTIGIGAGPSCDAQVLVIHDLLGI
                     NERVPRLAKKFADLRGVMTDAVEAFMREVDAGRFPDEDHSYS"
     gene            2619..3341
                     /locus_tag="VF195_10315"
     CDS             2619..3341
                     /locus_tag="VF195_10315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581251.1"
                     /translation="MTGDDERDDTLAERAALVSKLTHEIRGPVSTIRGLAGTTLAHYD
                     RLDDGERREFLELIRHEAERLERTVEQVALALKLDAGTLPFDIRMQDLGVIVRAAADA
                     AETGDHPIEVQTHEQVDAAVDGIQVAFVIRQLLENAAIFSPTGSPISVRLTSEGDHAL
                     IRVTDRGPGIPEGERDGVFERFAGWRPTGYEDRPGTGLGLFICRAIVEEHGGEASIVD
                     GPTEGTMLVVRLPLEARMAGSR"
     gene            3338..3994
                     /locus_tag="VF195_10320"
     CDS             3338..3994
                     /locus_tag="VF195_10320"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581252.1"
                     /translation="MTTLLICDDHKVLTDALSTVVGLDEDLQLVAAPVHDPETAIELS
                     AEHLPDVVLMDIVFKGGMSGIEATRRIKEISPATKVVIMTAHDEDRLLVEAVEAGASG
                     FLGKGEAVEEVLAAAKAAAEGEVLIDPATLTRLLAQVAREREAHREASMLLDDLTDRE
                     REILQLLAEGMRNEGIATKLFISPQTVQTHVRNILGKLRVHSKLEAVAFAVKHGAIVV
                     "
BASE COUNT          614 a         1391 c         1350 g          669 t
ORIGIN      
        1 ggcccacacg acgggcgcct actcctgagc tcgaacgtcg atcgtgatga cggggtcgac
       61 ctcggggtgc aggtggacgg tcacctcgtg cgtcccgacg gagcggatcg gctcgtcgag
      121 atggatgtcg cgccggtcga cggcgatacc cgtctccgcc gagagcgccc ccgcgatgtc
      181 ggcggccgtg accgatccga acagcttgcc ctcctcgccc gcccgcgccg tcaccacgac
      241 cggggtctgg atgatgcggg cagcgatcgc ctcgaactca cccttctccg ccttcgaccg
      301 cgactgatgg gcgcgtttga ggctctcggc gtggcgcacc gtccccttct cggctcgcac
      361 ggcgaggccg cgagggatca ggaagttccg cgcgtagccg tcggcgacgt ccgcgacatc
      421 gcccggatcg ccgaggcgtt cgaccgtctt ctgcaggatg accttcatcg cagcggtcct
      481 atcggctcga gtacggcagc agcgccatct cgcgagcgtt cttcacggcc gacgcgacgt
      541 cgcgctggtg ctgcacgcag ttccccgaaa cgcgccgagc gcggatcttg cctcggtcgg
      601 acacgaactt gcggagcgtc atcacgtcct tgtagtcgac gcgatcgacc ttgtccttgc
      661 agaactggca gtacttgcgc ttgccgcgct tctgccagcc tttgtcgtcc ttctcgcgac
      721 gccgcttggg gccgcgctcc ttcggtcctc ccctacccgc catcgagagg agtctcctcc
      781 ggttcggttc cgccgccgac cggtgcggcg accttctcgc cccagtcgcc ggagctcgac
      841 gccccgccgg agcgtgcctg gcgcgtcacc gatgccgtcg cccacttcag gctcggggcg
      901 acctcgtcgg cctcgacctc gatcgccgag cgcttatcac cgtcctggtt ctcccaggtc
      961 cggtagttga gccggcccgc gacgatcact cgcgtgccac gcgtgaggga ctccgccgcg
     1021 ttctccgcca tcgtccgcca ggcgttgatc gtgaaatacg acgtgtcgcc gtccttccac
     1081 tgaccgttcg cgtcctggaa ccgacggttg acggcgatcc ggaacttgca caccgcagcc
     1141 ccgttcgggg tgtagcggag ctctgggtca tcggtcaggt tgccgaccag ggtcacttgg
     1201 ttgtccgtcg ccatgttcgt tcactcccct tggggtgtct cgtctccgcc agccgcggag
     1261 gcgggggaag atgccatctg cttcttcttc tgcccacgtg gtgagggcag gacgatcacc
     1321 ttgtacctca ggatctcgtc cgccaggttc agcgtccgct cgagctctgc ctggacggaa
     1381 ggctccgccg agaacgtgac cacgacgtag tagccctcgt tctggtcgtc gatctcgtag
     1441 gcgaggcgac gccggcccca gcgctcgatg ttgcgtatcg ccccggaatg cttcgcggcg
     1501 accgtcgcga tccggtcggt cgccgtgccg accacggctt cgtcggccgc agcgggcagg
     1561 atgagcatgc tctcgtactc cctcaaaccc cacctcctct ggtctggacg gcccccctcg
     1621 aaggggagca ggaagtcctg agggtcgtcc agcagtgtac gggacctgcg cggacggtag
     1681 caccgaggta cgacatcggg tcggctagaa tcgctcgact gccgtgcact tcagaggagg
     1741 cgcgagcgat gaccacgatc cacgacctca aggcgtggaa ggcggacggg cggaggttca
     1801 cgatgctcac cgcctacgac ttccccaccg cccagatcct cgatcgagcc ggggtcccgc
     1861 tgctgttggt gggtgactcg gtggggaaca acgtcctcgg ctacgaggac accctgccgg
     1921 tcacgatggc ggagatgctc caccacaccc gagccgtcgc gaggggtgcc gagcgggcga
     1981 tggtgatcgg ggacctgccc ttcctctcct accaggtctc gatcgaggaa ggggtgcaca
     2041 acgccggccg cctcgtgaag gagggcggcg cgcacgccgt caagctcgag ggaccgcacc
     2101 tcgacctcgt ccatcggctg gtcgacatcg gcatcccggt gatggcgcac atcggcctga
     2161 cgcctcagtc ggtccatgcc atgggcgggt atcgggtaca gggccggggc gaagcagccc
     2221 accgcgtcgc ggatgaagcc gagcagctcg agaaggcagg cgcgttctcg atcgtcctgg
     2281 aaggtatgcc ggcggagctg gccggcgata tcaccggatc gctggagatc cccacgatcg
     2341 ggatcggggc gggtccgtcc tgcgacgccc aggtgctcgt gatccacgac ctcctgggga
     2401 tcaacgagag ggtgccgagg ctcgccaaga agttcgccga tctccgcggc gttatgaccg
     2461 atgccgtgga ggcgttcatg cgcgaggtcg acgcgggcag gttcccggac gaggaccact
     2521 cctattcgta ggcacccatc tgtgggtgct catccgtagg cgcctatcct tgacgccgtt
     2581 ccgactctgg ggcgccgttc ctccctagac tcgcgggcgt gaccggcgac gacgagcggg
     2641 acgacaccct cgcggagcga gcggctctcg tctcgaagct cacacacgag atccggggcc
     2701 ccgtctcgac gatccgcggc ctcgcgggca cgacgctcgc acactacgac cgactcgatg
     2761 acggcgagcg acgggagttc ctggagctga tccgccacga ggcggaacgg ctcgagcgca
     2821 ccgtcgaaca ggtggccctc gcgctgaaac tcgacgccgg gaccctccca ttcgacatcc
     2881 gcatgcagga tctcggcgtg atcgtgcggg ctgccgcgga cgccgcggag accggcgacc
     2941 atcccatcga ggtgcagacg cacgagcagg tcgacgcagc cgtcgacggc atccaggtcg
     3001 cgttcgtgat ccgccagctc ctcgagaacg ccgcgatctt ctcgccgacc ggttcgccga
     3061 tctcggtccg cctgacgtcg gagggcgacc acgcgctgat ccgcgtcacc gatcgcgggc
     3121 ccgggatccc ggagggcgaa cgggacggcg tcttcgagcg cttcgccgga tggcgcccga
     3181 ccggctacga ggatcgaccg ggaacaggac tcgggctgtt catctgccgc gcgatcgtcg
     3241 aggaacacgg gggcgaggct tcgatcgtgg acggccccac ggagggtacg atgctcgtcg
     3301 tccggcttcc cctggaggca cgaatggcgg gctcccggtg accacgctcc tgatctgcga
     3361 cgaccacaag gtcctgacgg acgccctgtc gacagtcgtc ggcctcgacg aagacctgca
     3421 gctcgtggcc gcgcccgtcc acgatcccga gacggcgatc gagctctcgg ccgaacatct
     3481 gcccgacgtg gtgttgatgg acatcgtctt caaggggggg atgtccggga tcgaggcgac
     3541 ccgccggatc aaagagatct cgcccgcgac gaaggtcgtg atcatgacgg cgcacgacga
     3601 ggatcgcctg ttggtggagg cggtcgaggc cggcgcctcg gggttcctcg gcaagggcga
     3661 ggccgtggag gaggtcctcg cggccgcgaa ggccgcggcc gagggtgagg ttctgatcga
     3721 tcccgcgacg ctcacgcggc tcctcgccca ggtcgcgcgc gagcgtgagg cgcaccggga
     3781 ggcctcgatg ctgctcgacg atctcaccga tcgtgagcgg gagatcctcc agctcctcgc
     3841 cgagggcatg cggaacgagg gcatcgccac gaagctgttc atcagccccc agacggtcca
     3901 gacgcacgtc cggaacatcc tcggcaagct ccgagtgcac tcgaagctcg aggcggtcgc
     3961 cttcgccgtg aagcacggcg cgatcgtcgt ctgagcccgc tcgcggtcgg gaactcctgc
     4021 ccgg
//
LOCUS       DASUYD010000184         5965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_109169, whole genome shotgun sequence.
ACCESSION   DASUYD010000184 DASUYD010000000
VERSION     DASUYD010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109169"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..689)
                     /locus_tag="VF195_10325"
     CDS             complement(<1..689)
                     /locus_tag="VF195_10325"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581253.1"
                     /translation="MSRAATRTRDHPAGAPAASTISVRELGMTRTRPSGEQVPAFEGL
                     TFDVARGEFVSLIGPSGSGKTSTLGVIAGLERPTHGEVLVDGRPITGPGPDRAVLFQE
                     PALFPWLSVRGNMDLALRLANVPPGERQERAATWLARVGADRAADRQPHELSAGMRQR
                     VALARALACDPPILLGDEPFGALDALARERLQDNVQRERIERAGRTTFLFATHNVREA
                     VLLADRVLVMSA"
     gene            complement(686..880)
                     /locus_tag="VF195_10330"
     CDS             complement(686..880)
                     /locus_tag="VF195_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010191865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YezD family protein"
                     /protein_id="HEX6581254.1"
                     /translation="MTDETPRSSSHDEPIDGEELTDEERRVLANVAGILRKIKYGTVV
                     LVVQDGKVVQIEMAEKFRLR"
     gene            1204..2253
                     /locus_tag="VF195_10335"
     CDS             1204..2253
                     /locus_tag="VF195_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0043199 - sulfate binding [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6581255.1"
                     /translation="MFLRSKHLAVLGIVPLLALSACGSNPAGAAADCAGLPDTPVISF
                     AAYSTPREVYGKIIPAFRDQWKAEHNGQDVIFQESYGASTSQAANVIAGYEADVVALS
                     LGPDVDEIADAGLITSDWTDSPDGGMVSTSVVVFDVRPGNPSGIEDWDDLAQPGVEVL
                     TPDPASSGGARWNLVSAWGAALRGHGGVSDGDEAGAAALLQGVLGNVVAFDKSARESI
                     KNFESGNGDVAITYENEVLTAQEAGLDDEAVYPPSSVLIENPVAIVDQNVDRHCVREV
                     AEAFVEFLHTPEAKEMYTDVGFLRSTDVQEAQAGGEGFPEIEDLFTVEDLGGWDALDD
                     ELFGDAGVVTEAMAG"
     gene            2256..3134
                     /gene="cysT"
                     /locus_tag="VF195_10340"
     CDS             2256..3134
                     /gene="cysT"
                     /locus_tag="VF195_10340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEX6581256.1"
                     /translation="MSGAAQTLSPSSGGSRSATVRSRDRTVAALRHLGLRGAAIVYLG
                     AFVVVPVAAVVTRGFGEGFTSLREALAIPHAWHAIWLTLITSGLAAILNAVMGVALAW
                     VLVRFRFRGRHTLSSLVDLPLAIPTLVTGLMIATLYGPNGAIGRELDGIGLQVIFTPV
                     GILLALCVVTLPLVVRNVQPVLQELDPAEEEAAATLGASGWTSFRRVVFPAIRPAVIG
                     GTLLTFARCIGEFGSVVLVSGNRPGETLTAPVFIFQLTGQFRPEEAAAVATLMFGISF
                     VLVFVTARLLRRREAA"
     gene            3131..4015
                     /locus_tag="VF195_10345"
     CDS             3131..4015
                     /locus_tag="VF195_10345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit"
                     /protein_id="HEX6581257.1"
                     /translation="MSVETPTAPPALAVEPRSGRRSYWRAGIAAICIAYVAVLLLLPL
                     IGIVITALDPGLSVVTETFSRPEVLHAFWLTFVITLVTVVVTTFFGLCVAWVLVRQRF
                     RGRALMNALVDLPFALSPVTVGLACVLLFGTGGWFADFFTARGIQIIFALPSMVLVTV
                     FICIPFTIREVVPVLEEIGLEEEDAARTLGASIWQTFRRVTFPNIRWALGYGIALTVA
                     RAIGEIGAVLIVSGLIQGSTETATIYIFNALEERQDAQAYVVALALAGISVVLLVLIE
                     GFRRHTQRGREAAGGIEV"
     gene            4012..5118
                     /locus_tag="VF195_10350"
     CDS             4012..5118
                     /locus_tag="VF195_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEX6581258.1"
                     /translation="MSTPIVVENLTKRFGDFEAVTDVSFTARAGSITALLGPSGSGKS
                     TVLRVIAGLERPTSGRILVGEEEVTAKSVQERAVGFVFQHYALFRHMTVIENVAFGLK
                     VRKEAAGTRDARVEELLELVQMSRFADRYPEQLSGGQRQRIALARALAPRPEVLLLDE
                     PFGALDARVRQDLRRWLDELHRELGVTSILVTHDQEEALELANQVVVMHEGRVEQAGS
                     PAEVYEDPLTAFVAGFVGAANVLHGRVIDGHVHLGTFRVPGAGHLADGADAAAYVRPH
                     DVRVSTEETGVRGTIERMATLGWLARLSIRLPGGETLVAHVPNEELGAVHEGDEIWLD
                     LRSPKAFGLGSGMQAPDLDVAAGVDDRTPDVVGA"
     gene            5136..5875
                     /locus_tag="VF195_10355"
                     /pseudo
     CDS             5136..5875
                     /locus_tag="VF195_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718833.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
BASE COUNT          847 a         2026 c         2105 g          987 t
ORIGIN      
        1 gccgacatca cgagcacgcg atcggcgagg agcaccgctt cgcggacgtt gtgcgtggcg
       61 aagaggaacg tcgtgcggcc ggcgcgctcg atgcgctcgc gctgcacgtt gtcctggagc
      121 cgctcccggg cgagcgcgtc gagcgccccg aacggctcgt cgcccagcag gatcggcggg
      181 tcgcacgcca gcgcccgcgc gagggccacc cgctgccgca tcccggccga gagctcgtgc
      241 ggctggcgat cggcggcgcg atccgctccc acccgggcga gccacgtcgc ggctcgctcc
      301 tgacgctcgc cgggcgggac gttcgcgagg cgcagcgcga gatccatatt cccccgcacc
      361 gagagccagg ggaacagcgc cggctcctgg aagagcaccg ctcggtcggg gcccgggccg
      421 gtgatcggcc ggccatcgac gagcacctcg ccgtgggtcg ggcgttccag cccggcgatc
      481 accccgagcg tcgaggtctt cccggagcca ctcggcccga tcaggctgac gaactccccg
      541 cgtgcgacgt cgaacgtgag gccttcgaac gcaggcacct gctcgccgga tggtcgtgtg
      601 cgggtcatcc cgagctcgcg cacggagatc gtcgaagcgg ccggagcgcc ggcgggatgg
      661 tcccgggttc gcgtcgcggc tcggctcatc ggagtcggaa cttctccgcc atctcgatct
      721 gcacgacctt cccgtcctgc acgacgagga cgaccgtccc gtacttgatc ttccggagga
      781 tgcccgcgac gttcgcgagg actcgccgct cctcgtccgt cagctcctca ccgtcgatcg
      841 gctcgtcgtg tgaggaggat cgtggcgtct cgtcggtcac ggctcgcatc ctagaagcgt
      901 cgaggccgcg gcgcgttcgc gccacggcct ccggtcgtcc ggtcagctgt cgatccgagt
      961 ctagcccgag atgttcgtcg gcgccgcgag ccagcgggta ccgcgcgcgg gttgtgacgg
     1021 aatcgtgacg agtggtgctt cctggcggca gatgtttgac acgacccctc cccgggcggt
     1081 atccatgcgg gcacgtaccg cccgcgacgc gggcgatgtc ctttcggttg accggaccac
     1141 cggagaccga cccacagcaa ccggtgggcg gctcacgtcc cggttcgatc gaaaggagct
     1201 tcgatgttcc tccgatccaa gcacctagca gttctcggca tcgtgcccct tctcgcgctg
     1261 tccgcgtgcg gctcgaaccc cgccggcgcg gccgccgatt gcgccggcct cccggatact
     1321 cccgtcatca gcttcgcggc gtacagcacg ccgcgcgagg tctacggcaa gatcatcccg
     1381 gcgttccgtg accagtggaa ggcagagcac aacggccagg atgtgatctt ccaggagtcc
     1441 tacggtgcgt ccacgtcgca ggctgcgaac gtgatcgccg gttacgaggc cgacgtcgtg
     1501 gcgctgtcgc tcggcccgga cgtcgatgag atcgccgacg cgggcctgat cacgagcgat
     1561 tggaccgact cacccgacgg cgggatggtc tcgacctccg tcgtggtgtt cgacgtccgt
     1621 cccggcaacc cgtcggggat cgaggactgg gacgacctgg cccagcccgg tgtggaggtg
     1681 ctcacacccg accctgcatc gagcggcggc gcacgatgga acctcgtctc cgcctggggt
     1741 gccgcgctcc gcggccacgg cggcgtgagc gacggtgacg aagccggcgc cgcggccctc
     1801 ctccagggcg tgctgggcaa cgtcgtcgcg ttcgacaaga gcgcccgcga gtcgatcaag
     1861 aacttcgagt ccggcaacgg cgacgtcgcg atcacctacg agaacgaggt gctcacggcg
     1921 caggaggccg gtctggacga cgaggccgtg tacccgccgt cgagcgtgtt gatcgagaac
     1981 ccggtcgcga tcgtggacca gaacgtcgac aggcactgcg tccgcgaggt cgcggaggcg
     2041 ttcgtggagt tcctgcacac tcccgaggcg aaggagatgt acaccgacgt cggtttcctc
     2101 cggtccacag acgtgcagga agcgcaagcc ggcggcgagg gcttcccgga gatcgaggac
     2161 ctgttcacgg tggaggacct cggtggctgg gacgcgctcg atgacgagct gttcggggac
     2221 gccggcgtcg tgaccgaggc gatggcgggc tgaggatgag cggcgcagcg cagacccttt
     2281 cgccttcttc tggcggatcc cgcagcgcga cggtgcggtc ccgtgaccgc accgtcgccg
     2341 ccttgcgaca cctcgggctg cgcggcgccg cgatcgtcta cctgggcgcc ttcgtcgtgg
     2401 tccccgtggc cgccgtcgtg acccgggggt tcggcgaagg cttcacctcg ctgcgagagg
     2461 cgttggcgat cccgcacgcc tggcacgcga tctggctcac cctgatcacg tcgggcctcg
     2521 ccgcgatcct gaacgccgtg atgggcgtgg cgctcgcctg ggtcctcgtc cgcttccggt
     2581 tccgcggccg gcacacgctc tcctcgctcg tcgatctccc gctggcgatc cctacgctgg
     2641 tgaccgggct gatgatcgcc acgctgtacg gaccgaacgg cgcgatcggc agggagctcg
     2701 acgggatcgg gcttcaagtc atcttcacgc cggtggggat cctcctcgcg ctctgcgtcg
     2761 tgacgcttcc tctcgtcgtt cgcaacgtcc agccggtcct ccaggagctc gaccccgcgg
     2821 aggaggaggc cgccgccacg ctcggcgcga gcggctggac gtccttccgg cgcgtggtct
     2881 tcccggcgat ccgcccggcg gtgatcggcg ggacgctgct cacgttcgcc cgatgcatcg
     2941 gcgagttcgg gagcgtcgtg ctggtctcgg ggaaccgccc gggggagacg ctgaccgccc
     3001 ccgtgttcat cttccagctg accggccagt tccggcccga ggaggcggcg gcggtcgcga
     3061 ccctgatgtt cggcatctcg ttcgtgctcg tcttcgtcac cgcgaggctg ctccggcgga
     3121 gggaggcggc atgagcgtcg agacccccac cgccccaccg gccctcgcgg tcgagcctcg
     3181 aagcggtcgg cgctcgtatt ggcgcgccgg catcgccgcg atctgcatcg cctatgtggc
     3241 cgtccttctc ctgctgcccc tgatcgggat cgtgatcacc gcgctcgatc ccgggctctc
     3301 ggtcgtcacg gagacgttct cgcgtcccga ggtgctgcac gccttctggc tcacgttcgt
     3361 gatcacgctc gtcacggtcg tcgtgaccac gttcttcggg ctgtgcgtgg cctgggtgct
     3421 cgtccgccaa cggttccggg gacgcgcact gatgaacgcg ctcgtcgacc ttccgttcgc
     3481 gctctcgccg gtcaccgtgg gattggcgtg cgtgctcctg ttcggcaccg gcgggtggtt
     3541 cgcggacttc ttcacggcgc gcgggatcca gatcatcttc gcgctgccct cgatggtcct
     3601 cgtcacggtc ttcatctgca tcccgttcac gatccgcgag gtcgtgccgg tgctggagga
     3661 gatcgggctc gaggaggagg acgccgcgcg caccctcggt gcctcgatct ggcagacgtt
     3721 ccgccgcgtg acgttcccga acatccggtg ggcgttgggg tacggcatcg cgctcacggt
     3781 cgctcgcgcg atcggggaga tcggcgccgt cctgatcgtg agcgggctga tccagggcag
     3841 caccgagacg gcgacgatct acatcttcaa cgcgctcgag gagcgacagg acgcgcaggc
     3901 ctacgtcgtg gcgctggcgc tcgccgggat ctcggtcgtg ctgctcgtcc tgatcgaggg
     3961 gttccgcagg cacacccaac gaggccgtga agcggcagga ggcatcgagg tatgagcaca
     4021 cccatcgtgg tcgagaacct gacgaagcgg ttcggggact ttgaggcggt gaccgatgtg
     4081 tcgttcaccg cgagagccgg ctcgatcacc gcgctgctcg ggccttcggg gtcgggcaag
     4141 agcacggtgc tgcgggtgat cgccggactt gagcgcccga cctcgggccg gatcctggtg
     4201 ggcgaggagg aggtcaccgc caagagcgtg caggagcgcg cggtcggctt cgtgttccag
     4261 cactacgcgc tgttccggca catgaccgtg atcgagaacg tcgcgttcgg tctcaaggtc
     4321 aggaaggaag ccgcagggac gcgcgacgcg cgcgtggagg agctcctgga gctggtccag
     4381 atgtcgcggt tcgccgatcg gtatccggaa cagctctcgg gaggacagcg gcagaggatc
     4441 gcgttggcgc gcgccttggc accccggccc gaggtgctcc ttctggacga gccgttcgga
     4501 gccctcgacg ccagggtccg ccaggacctc cggcgctggc tcgacgagct ccaccgcgag
     4561 ctcggcgtca cgagcatcct ggtcacccac gaccaggagg aggcgttgga gctcgcgaac
     4621 caggtcgtgg tgatgcacga gggtcgcgtg gagcaggccg gctcgcccgc cgaggtctac
     4681 gaggatccgt tgaccgcgtt cgtggccggc ttcgtcggcg ccgccaacgt gctccacggc
     4741 cgggtgatcg acggccacgt gcacctcggg accttccgcg tgccgggagc cggccatctc
     4801 gccgacggcg ccgacgcggc cgcctatgtt cgtccgcacg acgtccgggt ctccaccgag
     4861 gagaccggcg tgcgcggcac gatcgagcgg atggcgaccc tcggctggct cgcgcgactc
     4921 tcgatccgtc tccccggtgg agagacgctc gtcgcccacg tccccaacga ggagctcggc
     4981 gcggtccacg agggagacga gatctggctc gatctccgga gcccgaaggc cttcggtctc
     5041 ggatccggga tgcaggcgcc ggatctcgac gttgccgcag gtgtcgacga tcgaacgccc
     5101 gatgtggttg gcgcatagcc gatagattcg ggcgcgtgca cggcgacggc tacttcgacg
     5161 agcgcgtggc ggagcgattc gacgagaggt acgcgaatct cgccagcccc gccatcgtcg
     5221 atcccatagt cgccttccta gcggatctgg cacgaggcgg tggggcgctc gagctcgggg
     5281 tcgggaccgg ccgcatcgta ctcccgctcg ccgagcgcgg ggtgccggtg cgggggatcg
     5341 agctgtctac ggcgatggtc gaacggctgg gcgccgaagc ccgatagcga ggacatcggt
     5401 gtgacgatcg gcgacttcgc gacgacccgg ttggaagggt cgttttccct cgcgtaccta
     5461 gtcgccaaca cgatcgggaa cctgacgacg caggagggcg caggtcgcgt gcttccgcaa
     5521 cgcggccgcg cacctcgacc acggtggaac cttcgtgatc gaggtcggca tgccggagct
     5581 gcgccggctc ccgcccggcg agcggggcaa cgtcttccgt atagacgagg atcgctgggg
     5641 catcgacgag tacgacgtcg cgacgcaggg actgatctca catcatttct ggatccagga
     5701 gggtcgggcg gagcacttct cgatgccgtt ccggtacgtc tggcccgccg agctcgatct
     5761 gatggcgcag ctcgccggga tgacgctgcg agaacgatgg gaagggtgga cgaaggagcc
     5821 ctttacgagc gagagccgat cccacgtgtc ggtctgggag aagccggcgg agtagtcacg
     5881 gtggccttcg gctccggtgg tacgttcgga gtcgtgagtc cctctgacct acagcccacg
     5941 gatctgatcg acatcgacca cctcc
//
LOCUS       DASUYD010000185         2664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_552167, whole genome shotgun sequence.
ACCESSION   DASUYD010000185 DASUYD010000000
VERSION     DASUYD010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552167"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VF195_10360"
     CDS             <1..736
                     /locus_tag="VF195_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX6581259.1"
                     /translation="FLAEGLITDVVRIVKTGKEASVHLCLANRSTTGEDLLALKVYHP
                     LDRRDFRDESIYRDGEWIEERRIRAALEKKSRFGREVQGGLWVSREWETLRELSASGA
                     PVPRPVASTGDAILMSFVGDHERAAPQLRSCKLDAVEAQDLFEQVLRAIERMLYGNVI
                     HGDLSAYNVLVWEGRATIIDLPQAVDPRKNRHAPALLERDVQRICEHFARSGVRSAPA
                     ELAGDLWTSWQFADLVPEELRTGIEM"
     gene            1073..1717
                     /locus_tag="VF195_10365"
     CDS             1073..1717
                     /locus_tag="VF195_10365"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6581260.1"
                     /translation="MEQLNMSRRWFEDLESLSRRVLTTVLFTDVVDSTGRLLELGDRM
                     WTELLGSHHTRFLERLGRFGGREVDAAGDGFLAAFDVPTYAVAFACSFHDDVIELGLE
                     VRTGVHTGECELEDGRLRGVAVHVGARLAGIARPGEVLVSATVRDVVAGGELRFADRG
                     LHELRGVPGQRRLFAALWNGTDASVQERRGRSRAAVGVSHRRQPKQLMDTADVG"
     gene            complement(1755..2135)
                     /locus_tag="VF195_10370"
     CDS             complement(1755..2135)
                     /locus_tag="VF195_10370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581261.1"
                     /translation="MLLGNEECWAVETSRDGSQWRWLGKAWKSPNESVLMHVSVRFIR
                     FRQLVPTESKAWSEPLETEGRLPMTMVRMEDEQREDLWPGDEHLGLPMLLPGGETGRL
                     LGFEYAPDGSSWRYTLEFRGAREG"
     gene            complement(2344..>2664)
                     /locus_tag="VF195_10375"
     CDS             complement(2344..>2664)
                     /locus_tag="VF195_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015097816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-hexose 4-ketoreductase"
                     /protein_id="HEX6581262.1"
                     /translation="DVKQIVDLLMKRVTEQLKAKDLEIELTDAAKTVLAEKGFDPSLG
                     ARPLRRTIQRFVEDPLSEKLLWKEFRAGQTIIVDARDGEIVFEGAGVPPDVPPVELAG
                     SSEA"
BASE COUNT          416 a          897 c          897 g          454 t
ORIGIN      
        1 cttcctggcc gagggcctga tcaccgacgt cgtccgcatc gtgaagaccg gcaaggaagc
       61 ctccgtccac ctgtgcctgg ccaaccgttc gaccaccgga gaggacctcc tcgcgctgaa
      121 ggtctaccac ccgctcgacc gtagggactt ccgcgacgag tcgatctacc gcgacggcga
      181 gtggatcgag gagcggagga tccgcgcggc cctcgagaag aagtcgcgat tcggccgaga
      241 ggtccagggc ggcctctggg taagccgcga gtgggagacg ttgcgcgagc tgtcggcctc
      301 gggggcaccc gtgccgaggc ccgtcgcgtc caccggcgat gccatcctga tgagcttcgt
      361 cggcgaccac gagcgggcgg cgcctcagct gcgttcgtgc aagctcgacg ccgtcgaagc
      421 gcaggacctg ttcgagcagg tcctgcgggc catcgaacgc atgctgtacg gcaacgtgat
      481 ccacggcgac ctgtccgcgt acaacgtgct ggtgtgggag ggtcgcgcga cgatcatcga
      541 cctgccccag gcggtcgacc ctcgcaagaa ccggcacgct cccgcgctgc tcgaacgcga
      601 cgtgcagcgc atctgcgaac acttcgcccg atccggcgtg cgctccgctc cggcagagct
      661 ggccggcgac ctgtggactt cgtggcagtt cgccgacctc gtgcccgagg agctgcggac
      721 cgggatcgag atgtgaccgt cgtgtgatcg tgctgaagga gcccttgcat cttggtgcgc
      781 gaaggcgtaa ggttcgcgat tcaccgacgt cgaaaggagg gcaagcgatg gatgtgactt
      841 ccaccatccc gaatatcgtc gcgtccctcc cgatgtcgtt ctcggcctga gccaaggtcc
      901 ggccccgggg aacgtggcga cctgttgtat cccggatttg gctgctcctc gcacaacccg
      961 gaaacacccg acttggaggc cgtcaacgaa cgtgtatccg tcacacctgg cacgcccgca
     1021 ccgcggatcg acgcatccgc ggtcgatgga gagaaggggg gtctgttccg agatggaaca
     1081 actgaacatg tctcgaaggt ggttcgagga cctcgagtcc ctctctcggc gcgtgctcac
     1141 gacggtcctg ttcacggacg tggtcgactc gaccggacgg ttgctggagc tcggtgaccg
     1201 gatgtggacc gagttgcttg gatcgcacca tacgcggttc ctggaacggc tcgggcggtt
     1261 cggagggcgc gaggtcgacg ctgcgggcga tggtttcctg gctgcgttcg acgtcccaac
     1321 ctacgccgtg gcgttcgcgt gctcgttcca cgacgacgtc atcgaactcg gtctcgaggt
     1381 tcgcaccggt gtccacacgg gcgagtgtga gctcgaggac ggcaggctcc gtggcgtggc
     1441 ggtccacgta ggtgctcgct tggcgggcat cgcacgcccc ggcgaggtcc tcgtctcggc
     1501 aaccgttcga gacgtggtgg cgggaggcga gctgcggttc gcggatcgtg gcctgcacga
     1561 gctacgcggc gtcccgggac agcgcaggct cttcgcggcg ctctggaacg ggaccgatgc
     1621 gtccgtgcag gagcggcgtg gacgatcgag ggcggcggtc ggagtgtcgc atcgtcggca
     1681 gccgaaacag ctgatggata ccgccgacgt cggatgaagg gcgtgcgggc tccggatcac
     1741 ccgggctcgc acgcctagcc ctcgcgggcg ccgcggaact ccagcgtgta cctccagctc
     1801 gagccgtcgg gtgcgtactc gaacccgagg agcctgcccg tctcgccgcc cgggagcagc
     1861 atcggcaggc cgaggtgctc gtcgccgggc cacaggtcct cccgttgctc atcctccatg
     1921 cgcaccatcg tcatcgggag ccttccctcg gtttcgagcg gctcactcca tgccttgctc
     1981 tcggtgggca cgagctgtcg gaatcggatg aaccgcaccg agacgtgcat caggacgctc
     2041 tcgttcggcg atttccatgc cttgccgagc caacgccact ggctgccgtc ccgcgaggtc
     2101 tcgaccgccc agcactcctc gttcccgagc aacattggcc gagagtaacc gagtctcctg
     2161 aggaacctca ggcagaggcc agccgtgggt cgatcgccgg ttcgggatcg acgatcacaa
     2221 gctcctgacc cgacgtgtga gccgacccgg gacaccgaac ggcccggcag ccacatggtt
     2281 gccgggccct cggtctttcg gggttcggcg acgtggcgcc ggacccactg gtctgctacc
     2341 cgctcaggcc tccgacgaac ccgcgagctc gaccgggggg acgtctggcg ggacgcccgc
     2401 gccctcgaac acgatctcgc cgtcccgcgc gtccacgatg atcgtctggc cggcgcggaa
     2461 ctccttccac aggagcttct cggacagcgg atcctcgacg aagcgctgga tcgtccgccg
     2521 tagcggccgg gcgccgagcg acggatcgaa ccccttctcg gccaggaccg tcttcgcggc
     2581 gtccgtcagc tcgatctcga gatccttggc cttcagctgc tcggtgaccc gcttcatcag
     2641 caggtcgacg atctgcttca cgtc
//
LOCUS       DASUYD010000186         4023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1434559, whole genome shotgun sequence.
ACCESSION   DASUYD010000186 DASUYD010000000
VERSION     DASUYD010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1434559"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..627)
                     /locus_tag="VF195_10380"
     CDS             complement(<1..627)
                     /locus_tag="VF195_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain-containing protein"
                     /protein_id="HEX6581263.1"
                     /translation="MTGGSGQGWFEADALRRLVPIVALTVALVAVVTQPSSPADLLLA
                     GVPVAAFYVWAWVPKAPLLVLSVAVIVPVVVAQRSGELEPLMFEASVLAFVVGRWSPS
                     LRSAVPLGLLAAATPVLVALMQDPSEVAVAIWIMGIAFPWAMSRAFVRQEQLADQLED
                     TRRELAQQALVDERRQIARDVHDFVGHGLAAVMLQVTSARHVLRRDPGA"
     gene            complement(711..1505)
                     /locus_tag="VF195_10385"
     CDS             complement(711..1505)
                     /locus_tag="VF195_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6581264.1"
                     /translation="MRTGWIVTASLMLMGSACGGEQRAEPPTVPSSSPAPSEAATSRF
                     PAPDRADLLAMSEPVRLHGDGGVRLEGRLFGSGDVGVAPAHMGGGDQSQWFGLAGLLA
                     DNGYRVLTYNRRGSCPGGELGCSGGHDVGGWRDLAFVVERLREAGAQRVVVGGASLGA
                     MESLYALSRGLDAEGLIWVAGVDLYGGLTVSEQTDHLRIPKLFMAGEVDGEAGDLLQV
                     FDQTAPEPMEVVTLDTGEHGTDILDYADVAVADAFRQAVLDFLARI"
     gene            complement(1555..1938)
                     /locus_tag="VF195_10390"
     CDS             complement(1555..1938)
                     /locus_tag="VF195_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6581265.1"
                     /translation="MEEHLQPRVVRAQQRMRQAALQTAGVLREQAFSTADVWIGIVTT
                     EPGAMSAWHHHGDHDTYAYVVSGLKRIEYGPGGTGSLVARPDDFIHLPKGLMHREGNP
                     SEEVSYSIAFRLGRGPATINVEGPP"
     gene            2039..3100
                     /locus_tag="VF195_10395"
     CDS             2039..3100
                     /locus_tag="VF195_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX6581266.1"
                     /translation="MRWIALPVGIVIVVWTLLDVFRTLVMPRAARGRVRLARILFRPI
                     WRPWRWIGVRGKTTQARERILSAAAPFLFFVLLIGWVSLALLGYALILWSPAFVDGIG
                     SGGDSFSNALYVSGTSLFTLGSVAGITGWTRAIVVAEAATGLGLFAVVIAYLPVLYQA
                     FNRREVGVLLLDARAGSPPSGPELLHRMGSAGAASSLHGLFAEWERWVADVLESHMSY
                     PLLTLFRSPHDNSSWVTSLGSVLDAATLLLTAVEDEPDERAILMYGTGVHAVEDLFYY
                     FRLTERETVIQRDEFEDVLGDMRDDGFAVRPLDDAFDRFTEKRAKYAPRLDALALLLA
                     ATPTLWIGDRSFLGARTEH"
     gene            complement(3115..>4023)
                     /locus_tag="VF195_10400"
     CDS             complement(3115..>4023)
                     /locus_tag="VF195_10400"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX6581267.1"
                     /translation="VEEVSEGASTWSIPASPWWQTAAGVFAGGTLAFVADAALAGAVF
                     TTLATGTQLLSSDLSMNFLQPAGPSSERLVAKGTVIDVGRRQGLSEARVEDAAGRLLA
                     HATSRCVLQPLPFEPPEPPDVSAVVDPPRYEAPDLYLRPVEGEVVPQAVWDETSGLDL
                     ATLWQKGERIASPNCLLLGLRFVEVAEQSVTLAMPASLWFCTGFGMFYGGILTALADS
                     AINTAVTTTLPPATSFGTLDLKVNFLRPVTPDGRDLFARAAVEQRGRTIAVSTARIED
                     ADGRLVAMASGSSMISEGRPWPTPAG"
BASE COUNT          647 a         1413 c         1358 g          605 t
ORIGIN      
        1 ggccccgggg tcgcggcgca ggacgtgccg agcactcgtg acctgcagca tgacggctgc
       61 caacccgtgg cccacgaagt cgtgaacgtc acgcgcgatc tgacgccgct cgtcgaccag
      121 cgcctgctgg gccagctcgc gtcgggtgtc ttcgagctga tccgcgagtt gctcctgccg
      181 aacgaaggcg cgagacatgg cccaagggaa ggcgatgccc atgatccaga tggcgacggc
      241 cacctccgac gggtcctgca tcagggcaac cagcaccggc gtggccgccg cgagcagacc
      301 gagcggcacc gcgctcctga gcgacggtga ccatcgcccg acgacgaagg cgaggaccga
      361 tgcctcgaac atgagcggct cgagctctcc cgagcgctgc gcgacgacca ccgggacgat
      421 cacggcgacg gagaggacca acaagggcgc cttgggcacc caggcccaga cgtagaaggc
      481 ggcgaccggc acgcctgcga gcaggaggtc ggccggggaa gagggctgcg tgaccacggc
      541 aacgagggcg accgtaagcg cgacgatcgg gaccaggcgg cgaagcgcgt cggcctcgaa
      601 ccagccttgg cctgacccac cagtcacgcg cgggactgta gcgtggcttc ctggttccga
      661 catcgtccta ggaacggagc cgagcactcc gaccgcaggt ggagctcgga tcagatgcgc
      721 gcgaggaagt cgagcaccgc ctgcctgaac gcgtcggcga ctgcaacatc cgcgtaatcg
      781 aggatgtccg taccgtgctc gcccgtgtcc agggtaacga cctccatcgg ctcaggagcg
      841 gtctggtcga agacctgaag gagatcgccg gcctcaccgt cgacctcacc cgccatgaac
      901 agcttcggga tccgaagatg gtccgtttgc tccgagacgg tgaggcctcc gtagaggtcg
      961 accccggcca cccagatgag cccttcagcg tcgagtcccc tgctgagcgc gtacagggac
     1021 tccatcgcgc ccaggctggc gccgcccacg acgacccgct gcgctccggc ctcgcgcagg
     1081 cgctcgacga cgaacgcgag gtccctccag ccccccacat cgtgcccgcc ggagcaaccg
     1141 agctcaccac ccggacagct cccccggcgg ttgtaggtga ggacgcgata tccgttgtcc
     1201 gcgagcagtc ccgcaagccc gaaccactgg gactggtccc cgccgcccat gtgagcgggg
     1261 gcgaccccca cgtcaccgga gccgaagagc cggccctcca aacgcacccc accatcacca
     1321 tgcaggcgca cgggctcgga catcgccaac aggtcggcgc ggtccggcgc cgggaaccga
     1381 gaggtcgccg cctcggacgg agccggcgag gacgacggca ccgtgggagg ttcggctcgc
     1441 tgctctcctc cgcacgccga ccccatgagc atcagcgaag cggtcacgat ccagccggtt
     1501 cgcatgccga agaggtatac gcctacgcga ccgccgccgg ttgtcgcact ccactcaggg
     1561 tggcccttcg acgttgatcg tcgcggggcc acgtcccaac ctgaacgcga tcgagtagga
     1621 gacttcttcg ctcggattcc cctctcggtg catcaacccc ttggggagat ggatgaagtc
     1681 atctggtcgt gcgacgagtg acccggtgcc acccggcccg tactcgatcc gcttcagccc
     1741 gctcacgacg tatgcatacg tgtcgtgatc gccgtggtga tgccaggctg acatcgcgcc
     1801 aggctcggta gtcacgatgc cgatccaaac gtcggctgtg gaaaaagcct gctcgcgaag
     1861 gacaccggcg gtctgcaagg cggcctgccg catcctctgc tgagcccgca cgaccctggg
     1921 ttggaggtgc tcctccatcg gcctcgcgtc atccgacgcc cgagctcgag agtacccgcg
     1981 aacccactcc tgctaaggaa ctcccggatg cgacggaccg accggtaggc ttcggcggat
     2041 gcggtggatc gcactcccgg tggggatcgt gatcgtcgtc tggacgctac tcgatgtgtt
     2101 ccggaccctc gtcatgccgc gagcggcacg cggacgggtt cgtctggccc ggatcctctt
     2161 ccggccgatc tggcgaccct ggaggtggat cggcgtacgc ggcaagacga cccaggctcg
     2221 cgaacggatc ctgtccgcgg cggctccctt cctcttcttc gtgctcctga tcggatgggt
     2281 ctccctcgca ttgctcggct acgcgctcat cctgtggagc cccgcgttcg tggacgggat
     2341 cggctcgggc ggggattcgt tcagcaacgc cctgtacgtg agcgggacgt cgttgttcac
     2401 gctcggatcc gtcgcgggca tcacgggatg gacccgagcc atcgtggtcg ccgaggcggc
     2461 gaccggtctc gggctgttcg cggtggtgat cgcctacctc cccgtgctgt accaggcctt
     2521 caaccgccgc gaggtcggcg tgctgttgct cgacgctcgg gcgggatctc ccccctcggg
     2581 ccccgagctg ctccaccgta tgggcagcgc cggcgccgcc tcgtcgctgc acgggctgtt
     2641 cgcggagtgg gagcggtggg tggccgacgt cctcgagtcc cacatgtcct atcccctcct
     2701 caccttgttc cgttcgcccc acgacaacag ctcgtgggtc acctccctcg gctccgtgct
     2761 cgacgcggcc acgctcctgc tcaccgcggt cgaggacgag cccgacgagc gggcgatcct
     2821 gatgtacggg acgggcgtcc acgcggtgga ggatctcttc tactacttcc ggctgacgga
     2881 gcgggagacg gtgatccagc gcgacgaatt cgaggacgtg ctgggagaca tgcgggacga
     2941 cggcttcgcg gtccggccgc tggacgatgc gttcgatcgt ttcacggaga agcgagcgaa
     3001 gtacgcgccg cggctggatg cgctcgcgtt gttgctggcc gcgacaccca ctctgtggat
     3061 cggcgatcgt tcgttcctcg gggcgcggac ggagcactga gcggggccgc gggcttaccc
     3121 ggccggtgtc ggccacgggc ggccttccga gatcatcgag cttcccgagg ccatcgcgac
     3181 gagcctgccg tcggcgtcct cgatccgcgc cgtcgagacc gcgatcgtcc gcccacgctg
     3241 ctcgacggct gcccgagcga ataggtcgcg cccgtcgggc gtgacggggc gcagaaagtt
     3301 caccttcagg tcgagcgttc cgaacgaagt cgcgggcggc agcgtggtcg tcaccgccgt
     3361 gttgatcgcc gagtccgcga gcgcggtgag gatcccaccg tagaacatgc cgaagcccgt
     3421 gcagaaccag agggacgcgg gcatcgcgag cgtcacgctc tgctcggcga cctcaacgaa
     3481 ccgaagtccg agcaagaggc agttcgggga ggcgatccgc tcccccttct gccagagcgt
     3541 cgcgaggtcg aggccgctcg tctcgtccca gacagcctgc ggaacgacct ccccctcgac
     3601 cggacgcagg tagaggtcgg gcgcctcgta ccggggagga tcgacgacgg ccgagacgtc
     3661 cggcggctcc ggtggctcga acggcagcgg ctggagcacg caccgcgagg tcgcgtgggc
     3721 gaggagcctt ccagcggcat cctccacgcg ggcttccgag agcccctggc gacggccgac
     3781 gtcgatgacg gtccccttcg cgaccagacg ctcgctcgac ggcccggccg gctggaggaa
     3841 gttcatcgac aggtccgagg acagtagctg cgtccccgtc gccagcgtcg tgaagacggc
     3901 gcccgccagc gctgcgtccg cgacgaacgc gagcgtgcct cccgcgaaga cgcccgcggc
     3961 agtctgccac caggggctcg cggggatcga ccacgtggag gcgccttccg acacctcctc
     4021 gac
//
LOCUS       DASUYD010000187         4092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_333225, whole genome shotgun sequence.
ACCESSION   DASUYD010000187 DASUYD010000000
VERSION     DASUYD010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333225"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VF195_10405"
     CDS             <1..456
                     /locus_tag="VF195_10405"
                     /inference="COORDINATES: protein motif:HMM:NF015665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX6581268.1"
                     /translation="ERINDEAIVAAVEKVKDALWNLEDKRIALLGLSFKPGTDDVRLS
                     PALALARKLIDEGAEVVGYDPEAGPNAKSDLPELVVAPGVYDALEGAHCVVVCTEWDE
                     FRSLDLDRAKELLAHPIMVDGRNLFEPAEVRGRGFVYYGTGRASDPGMH"
     gene            458..1426
                     /locus_tag="VF195_10410"
     CDS             458..1426
                     /locus_tag="VF195_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011437927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEX6581269.1"
                     /translation="MPRAVVTGGAGFVGSHLCERLLHEGWDVVCFDSLLTGRTANLAD
                     ASADQRFRFESHDVTNPFSVEEDVDWVLHFASPASPTDYLSHPIHTLKVGALGTLNAL
                     GLAKAKGAGFLLASTSEVYGDPKVHPQPEEYWGNVNPIGPRGVYDEAKRFAEAMAMAY
                     LRAHDVPVKIARIFNTYGPRLRRGDGRAVPTFIEQALEAEPITVHGDGSQTRSLCYVD
                     DLVEGIWLLLASDVVGMPVNLGNPEEVKVLDLGRTIALLAGSRSAVIFTERPIDDPEV
                     RCPDITRATSLLGWVPRIPLTDGLTRTIAWARETWRERDVAHVPDG"
     gene            1561..1839
                     /locus_tag="VF195_10415"
     CDS             1561..1839
                     /locus_tag="VF195_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEX6581270.1"
                     /translation="MKRLGIAVLGSLSFTLVSAASAFAQSDIPPNVAGEVVQPAGEVV
                     QPPGAAPGTTAFTGTDITVWMVVAAAMLLLGIGLLLAARRRARGAQIT"
     gene            complement(1882..3837)
                     /locus_tag="VF195_10420"
     CDS             complement(1882..3837)
                     /locus_tag="VF195_10420"
                     /inference="COORDINATES: protein motif:HMM:NF024594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HEX6581271.1"
                     /translation="MVVLGLILGTVGLLALSLTITVREVREDLLGGRSAMEQGRAELF
                     AGDAEAASDSFREGRQLFDRAENRTNGFVFRAVGWLPIVGRTSDAITAVADSAGMAAD
                     ATIVLSDAAAQIPGGLSGLAPSDGGLDIDRIPPLARAATESDELMSAALSRLEGAPTS
                     LLLGPVGVARREAEEELSATSESIHAASLLLRRLPTFLGAEQPQRFFFGAQNPAELRG
                     TGGLIGAYSILRIDAGRFHFSPYVPIHNLARPPLRSIPSPNEDYSANYDQFRRGGRFW
                     TSINVMPDFPSVAQAILNSYEAATGTRLDGVILADPFAEAALLRATGPVQLPGYDVEI
                     DADNVVAFATNEAYSLFTDSVRRKRLLGDVATAAFERFIDQPSADHQDLEQLLDAAGE
                     RHIQAYSEDPMMQQGLRATPVGGALRPRGADDDLLSVVVNSAAGSKVDFYQERNVSYA
                     VRLDDDGSAAAEFELTLRNDAPTSGQPPYVIGPFRSENAGSILRTLQAGESVALVNVY
                     CGADCIPLSARMDGVPVEVTTDVDLGMRYIQDYYSIRSGEQKTLQLSWDESSAWDGNG
                     SGGTYRMTFTNQVTIRPATLHLRIQPPDGMRIVSASPPLRIVGGAAVYDGEPGQRLDV
                     VIEFGPPLPVRLWRNVMRFLSTPVVEI"
BASE COUNT          697 a         1316 c         1384 g          695 t
ORIGIN      
        1 gagcggatca acgacgaagc gatcgtggcg gcggtcgaga aggtgaagga tgcgctgtgg
       61 aacctcgagg acaagcgcat cgccctgctg ggcctttcct tcaaacccgg gaccgacgac
      121 gtgcgcctct ccccggccct cgcgctcgcc aggaagttga tcgacgaggg cgccgaggtg
      181 gtcggctacg atccagaagc cggacccaac gcgaagtctg accttccgga gctggtcgta
      241 gcgcccggtg tctacgacgc cctggaaggc gcgcattgcg tggtcgtgtg caccgagtgg
      301 gacgagttcc gatcgctcga cctcgaccga gcgaaagaac tcctcgcgca tccaatcatg
      361 gtggacgggc ggaacctgtt cgagccggcc gaggtgaggg gaagaggctt cgtgtattac
      421 ggcaccgggc gagcgtctga cccggggatg cattgacgtg ccgagagcgg tcgtaaccgg
      481 cggcgccggc ttcgtcggct cccatctctg cgaacggctc cttcacgagg gatgggacgt
      541 cgtctgcttc gactcgcttc tcaccggccg cacggcgaat ctcgccgacg catcggcgga
      601 tcagcgcttc cggttcgagt cccacgacgt tacgaacccg ttctccgtcg aggaggatgt
      661 cgactgggtg cttcacttcg cgtctcccgc gtcaccgacc gactaccttt cccacccgat
      721 ccacacgctg aaggttgggg cgctcgggac gctcaacgcg cttgggctcg cgaaagccaa
      781 gggggcgggg ttcctcctcg cctcgacgtc cgaggtatac ggcgacccaa aggtccatcc
      841 gcagccggag gagtattggg gcaacgtcaa tccgatcggc ccacgtggcg tctacgacga
      901 ggcgaaacgc ttcgccgaag cgatggccat ggcgtacctg cgtgcccacg acgtaccggt
      961 caagatcgcg cgcatcttca acacgtacgg cccgcggctc cgacggggtg acggcagggc
     1021 cgttccgacg ttcatcgagc aggcgctgga ggccgagccg atcaccgtcc acggggacgg
     1081 ttcgcagacc cgatccctct gctacgtgga cgatctggtc gaggggatct ggttgctctt
     1141 ggcctctgac gtcgtcggga tgcccgtcaa cctgggcaac ccggaggagg tcaaggtgct
     1201 cgacctcgga cgaacgatcg ccctgctcgc aggaagccga tcagcggtca tcttcaccga
     1261 gcggcccatc gacgacccgg aggtccgctg ccccgacatc acccgagcga cgtcgttgct
     1321 cgggtgggtg cctcgcatcc cactgaccga cggtctgacg cgaacgatcg cctgggcccg
     1381 cgagacctgg cgcgagcgtg acgtcgcaca tgtaccggac ggttgaaggt tcgacccacg
     1441 gggcacggga gggaagcagg cagggacgag gtgttgtagg attcgtcaac gatcgcgaag
     1501 aacccgagaa gaacccgatg aggggaaggc aagtccggtc ggttgggtgg aagggacggt
     1561 atgaagaggc tcgggatcgc ggtgttggga agtctgtcgt tcacgctggt gtcggcggcc
     1621 tcggcgttcg ctcaatccga catcccgccg aacgttgccg gagaggtcgt gcagccggcc
     1681 ggagaggtcg tgcagccgcc tggtgccgcg cccggcacga cggcgttcac cggcacggac
     1741 atcaccgtgt ggatggtcgt ggcggcggcc atgctcctgc ttgggatcgg gttgctgctc
     1801 gctgcgaggc ggcgagcgag gggcgcccag atcacctgat cacctgatcg cccggtgcgg
     1861 gttgtcaatc ggggcgcgtc gctagatctc gaccaccggg gtggacagga agcgcatcac
     1921 gttgcgccac agtcgcacgg gtaacggtgg cccgaactcg atcaccacat cgagacgctg
     1981 acccggttct ccgtcgtaga cggccgctcc gccgacgatc cgtagtggag gactcgccga
     2041 gacgatgcgc atgccgtcag gtggctggat ccgaaggtgg agcgtcgccg gacggatcgt
     2101 gacctgattc gtgaaggtca tgcggtaggt tccgccggaa ccgtttccgt cccaggcgct
     2161 cgattcgtcc catgagagct ggagcgtctt ctgctctccg cttcggatcg agtagtagtc
     2221 ctggatgtat cgcatcccga ggtcgacgtc ggtcgtgacc tcaacgggaa caccgtccat
     2281 ccgggcgctc agcgggatac agtcggcccc gcaatagacg ttgaccagcg cgacgctctc
     2341 gccggcctgc agggtccgaa ggatggatcc ggcgttctcc gatcggaacg gtccgatgac
     2401 gtacggcggt tgccccgagg tcggtgcgtc gttgcgcagt gtgagctcga actcggcggc
     2461 ggccgagccg tcgtcatcga gcctgaccgc gtagctgacg ttgcgctcct ggtagaagtc
     2521 gaccttcgac cccgccgcac tgttcacgac caccgagagg aggtcgtcgt cggccccgcg
     2581 gggccggagc gcaccaccga ccggggttgc gcgcaggcct tgctgcatca tcgggtcttc
     2641 cgaataggcc tggatgtgcc gttcgccggc cgcgtcgagt agctgctcca ggtcctggtg
     2701 atccgccgac ggttgatcga tgaagcgctc gaaggcggct gtcgcgacgt ccccaagcaa
     2761 ccgtttgcgc ctcaccgagt cggtgaagag cgagtacgcc tcgttcgtgg cgaaggcgac
     2821 gacgttgtcc gcgtcgatct cgacgtcgta gccgggaagc tgaaccggtc cggtggccct
     2881 gagcagcgca gcctccgcga acgggtcggc gaggatcacc ccatcgagcc tcgtacccgt
     2941 ggccgcctcg tacgagttca gaatcgcttg ggccaccgat gggaagtccg gcatgacgtt
     3001 gatcgaggtc cagaaccgac caccgcggcg gaactgatca tagttcgccg agtagtcctc
     3061 attgggcgag gggatgctcc tcaacggtgg tcgtgcgagg ttgtggatcg ggacgtaagg
     3121 cgaaaagtgg aaccgcccgg cgtcgatccg caggatcgag tacgcgccga tcagtccacc
     3181 ggttcctcgc aactcggcgg ggttctgggc cccaaagaag aacctctgcg gctgctcggc
     3241 gccgaggaac gtcggcagac gtcgcaggag gagagaggcc gcgtggatcg actcgctcgt
     3301 cgcgctgagc tcctcctcgg cctcccgacg cgcaacaccg accgggccga gcaggaggga
     3361 cgtcggtgct ccttccaggc gcgagagggc ggcgctcatc agctcgtccg actcagtcgc
     3421 ggctcgcgcc agtggcggga tcctatcgat gtcgagtcct ccatccgagg gagcgagccc
     3481 ggaaagaccg ccggggatct gggcggcagc atcggataga acgatcgtcg cgtccgcggc
     3541 catgccggcc gagtccgcaa cggcggtgat cgcgtcactc gtacggccga cgatcggcag
     3601 ccaccccacc gctcggaata cgaatccgtt cgttcggttc tccgcccgat cgaacaactg
     3661 ccggccctcg cggaacgaat ctgaagctgc ttccgcgtcc cccgcgaaca gctccgcacg
     3721 gccctgctcc atcgccgatc tacccccgag aagatcttcg cggacctctc gcaccgtgat
     3781 ggtcaacgag agggcgagca gaccaaccgt gccgaggatg agaccgagga cgaccacggt
     3841 cctgcgacga ggccgtctcc gcttggaggg agattcagct gggtgctcat ccgggttcgt
     3901 cggggcggtt ccctgtcgcg taccgcttcg catcttcctt cttccgatcc gccgaggcgt
     3961 cccgacgcgg gacgccatgg tacccggggg gtttcgattc ggaggccccg cctgttgcct
     4021 cacgtcaaga tgtccttgaa ggatccatcg gttgcctcac caaagatgtc ctcgaaatct
     4081 ccgcccagga ga
//
LOCUS       DASUYD010000188        42037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_111112, whole genome shotgun sequence.
ACCESSION   DASUYD010000188 DASUYD010000000
VERSION     DASUYD010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 42037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42037
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111112"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF195_10425"
     CDS             <1..534
                     /locus_tag="VF195_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013229316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX6581272.1"
                     /translation="SAPAGGHGYQVHIGPMYSDARGSVKVVSTDPRVHPAIRFNYLST
                     DQDRREWVEAIRVARSILSQSAFGPFDGGELSPGPEVATDEQVLDWVRSDGETALHPS
                     CTCRMGTDDRSVVDPRTMRVHGVENLRVVDASAMPYVTNGNIYAPVMMLAEKASDLIA
                     GNTPLEPEHLEFYRHPD"
     gene            complement(562..1410)
                     /locus_tag="VF195_10430"
     CDS             complement(562..1410)
                     /locus_tag="VF195_10430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6581273.1"
                     /translation="MIFGLAAALGWGLADFFGAVSGRRIGSIGAVMSGQVLSAAFMTA
                     VLVATGTSLAPLGPDVWLVLLNGAAAAFAYLTHYRALELGPVAVVSPIGAGYAVVGVV
                     LAIVILGERPTGLALIGAGIAVAGVALVSTDLRKLRDGIKNHLPGLPWSVAAAVGFGV
                     AGFLLGWIADRAGWVAGLWGSRVAQIVFFLPLVLVFRRELSRLRPGFGLWIALLAGAA
                     DILGVVTFSVGSERGLNSIVLASSAVFPLIAVALSVIVFKERLVANQIVGIVLVVGGL
                     LLLGLG"
     gene            complement(1424..2242)
                     /locus_tag="VF195_10435"
     CDS             complement(1424..2242)
                     /locus_tag="VF195_10435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX6581274.1"
                     /translation="MSEPVLLVEDLGPIRRFTMNRPASLNALDRELVDAMSSALDEAA
                     ADEDVRVLILRGAGRAFSAGYDLNEDAAGGVLDARRWHADLQHSTEEMVKFLDHPKPV
                     IASVHSYCLAGGTDLMLACDLAIVADDAYFGYVDVRFGSGVVSMFLPWVVGVRAAKEL
                     LFTGEDRVSADEALRLGLVNRVVPRQELDAATLQLAEEIAKNEPFVIQTTKRAVNRAW
                     DVAGFRAALAANTELDVMIETANLPARDEFRRITAEEGLKAAIAWRDARFRGDA"
     gene            2424..3764
                     /locus_tag="VF195_10440"
     CDS             2424..3764
                     /locus_tag="VF195_10440"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6581275.1"
                     /translation="MVYHPRDPAHRSVSRREFLRRSLAAGVALPTSAAILAACGEDGN
                     GTPDPGAEDQIPRFGTLDNPVTLQTYDDNPPIDSGLEPEAGPLVIYNWEQYFWKQVLN
                     DFSKKYDVEVKYETFYNMEQAIQKFQSGEVDYDVFFPTIDYVPKLVAAKLLQPLNHDY
                     LPDTKNLWPVMQDPYYDQGSLYTLPYTIYHTGMAWRTDLVPVEPEDLAASGNPYEIFW
                     NADYAGKVGIYDAYREALSLGMYRDHLDETDLNPGDAAVIEAARDRLIELNDLVGVKT
                     TIDGAYAGVPEGKFGLHQSWSGDIQAGPWYTSDPKTEAPLLRYYWPARDGFGGTIDND
                     TYAIPTNAKNPVLAHLFLNFMMEEFNAIKNWTWVGYQVPFSAVNVDTIFEESYPGLEG
                     WFSWDYGAPKGEHWDNLKSTIVSEEDFDIGKRATGLPVEVDELWSTAYADFKAG"
     gene            3775..4713
                     /locus_tag="VF195_10445"
     CDS             3775..4713
                     /locus_tag="VF195_10445"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581276.1"
                     /translation="MSTPAGGTVGQRRWYYPALAMPGILWLLLLFIMPFYAIAAVAFG
                     TRDQIFALPIPAWNPLEWQFETFSETARELFASGGIQAQFTRTMLYVATAVVLCLLVG
                     YPVAYYIARYGARMKVLLLTLMIAPFWMSYLMRMLAWVNLLAVKPDDPGLFNRFLLLT
                     PFVNDPIAWSSGRVSHMTVILGLVYGYIPFFILPLYAALDRIDKSQLEASRDMGASPA
                     STFWRVTLPLSKQGILAGAVIITLPMFGDYYTPNLMTASPENTMIGNAIDLALQGGQG
                     TQPKGAGLTLLLSAFIGALMIYYLVSVSRAAKEAQR"
     gene            4710..5636
                     /locus_tag="VF195_10450"
     CDS             4710..5636
                     /locus_tag="VF195_10450"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581277.1"
                     /translation="MTTVEATVPRATAAPKVGWLRSLFSNPWGKPRFLSVWTWLYIIW
                     TIAPVVIAVQFSFNAGRSRSTFQGFSMQWYEAIWNEASDGFDDTLMPALVQSVKLALL
                     TMPIATALGVGLAIGLARWRGRGSGAANFLMLFPLVTPEIVMGASMFLIFSNLYHIRL
                     GTTAQLLGHVTFSLSYVVIVTRGRLFAIGRDYEEAAMDLGASPAGALRRVLLPLLAPA
                     ILSSFFIVFAISIDDFVISQFLCAQDCTTIPIKIYSAARNAGNPSLNALATIMMLLSL
                     VSITVAVLILRVFNKRQGTGRSAVEDFARFEI"
     gene            complement(5643..6764)
                     /locus_tag="VF195_10455"
     CDS             complement(5643..6764)
                     /locus_tag="VF195_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005144020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581278.1"
                     /translation="MPGGEVQLVDLVKRFGDFTAVDGINLRVDSGEFFSLLGPSGCGK
                     TTTLRMIAGFERPTDGQVVLDGMDVATVPPHKRNVNTVFQNYALFPHLNVEKNVAFGL
                     RYQDTSKVEIARMVGRSLELVRLTGYEKRRPNQLSGGQQQRVALARALILNPALLLLD
                     EPLGALDAKLRKALQIELKAIQEEVGITFIYVTHDQEEALTMSDRLAVMSDGRIEQIG
                     PPREVYEEPSTAYVADFLGVSNLMDATAYGPTDGGCTVRFGEFELVAGQGEPDAHGEV
                     KLSIRPERVDLQPSGGSGANRIPGMVERIVYVGSTMQVIVNLAPGEKLQVLVANEGEA
                     IPFEQGTAVSVHLPREALRVLPQGEVSEEGFSAAARAMR"
     gene            6840..8042
                     /locus_tag="VF195_10460"
     CDS             6840..8042
                     /locus_tag="VF195_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6581279.1"
                     /translation="MARAEAERGGEPLVRSRTLPLLAGYWSFGQFWGVWVILVLEYQR
                     QHALTDSRLGLMYSLLSLAAIGTMLAGAPRLQRLSLRASVPASLACLAVGSIGLAVQP
                     TSLVLVTFVVVGVGNGLIDVYLNVAAQREESRTSRPVLQWLHASYALGGVTGAAAAGV
                     LRTVGLDHRLGLAAAGVALLATAGWNARRAVDEPAAEATGALLSISALRRNPTLWIPA
                     LTVLFCFLVEGSMDIWSGLYLREQLGASAGAAGAAFVAFAGALFVGRLFAAKVLFGLG
                     ARRTIIISGIGGGIGGTIAVLSDTTVVVAAAFLLMGFAMSAAGPAGFSLVEGTSEDPS
                     AAIAAVATVGYTGFVWSPPLLGWIADTVDLRAAMGVIVFATVGITIMGFFARQKTAEG
                     GGPVEISR"
     gene            8112..9485
                     /locus_tag="VF195_10465"
     CDS             8112..9485
                     /locus_tag="VF195_10465"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX6581280.1"
                     /translation="MRVAIAVAVTLGLLAGEGLHPQRARALPARPDIVVLVTDDQHSG
                     TLGWMPVLRSYIARRGVRFTRAMVPTSVCCPSRASILTGEYAHTTKVWENTRGWARFR
                     RAGMEERTMAIWLRQAGYRTALVGKYLNIYHRAQPPPGWSTWHAFLENNAKYYGYELQ
                     HTNGSTTTHGFDAASYSTDVLRRYALRFIKATPSDTPLFLYLSPAAPHEPATPAPRHL
                     ALPASIESFWPPSARETDLSDKPRWIRDLPPVSSSAIHDSRIRQYRSLRSVDEAIAAI
                     IDLQRARRRLRNTLILVLSDNGEMWGEHRAMGKFLPYQGATRIPMAMAWPATLSGGRV
                     DTRLALNIDVAETVAVAASSPHPDVAGRNLLQRWERSGFVLEASGAQAFGGNGTNVGR
                     PPYCGWRTRRYLFVHYGNGREELYDYARDPWELRDRRGTAPDVQKRLRRRAREACSPV
                     PPGFAWG"
     gene            complement(9796..10605)
                     /locus_tag="VF195_10470"
     CDS             complement(9796..10605)
                     /locus_tag="VF195_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014877928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6581281.1"
                     /translation="MLEKFRSPLADDALSGRVAIVTGGGTGIGRATARELTRTGASVA
                     ICGRRPEPLEAVRTELEAAGSKVVTMPCDVREPEQVEGFLDVVGERLGSVDVLVNNAG
                     GQFAAPLEQIGLKGLRAVHRLNVDAPWHLTNRVAERWMIPARRGFVCFVGFSPRRGVP
                     LMAHSSAARAALENMASTIAIEWSKYGIRAVCVAAGLIQTEGMLQYGGQELVDEYAKS
                     VPMGRAGRAEEVAATIAFLASDGGAYVTGSTVTVDGGADAWGIGAAPPPIE"
     gene            10618..11475
                     /locus_tag="VF195_10475"
     CDS             10618..11475
                     /locus_tag="VF195_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6581282.1"
                     /translation="MKDIDVRGAGEIRLAARVHDAPSAGARGLLLLHGLASSQRIWDL
                     MLPRLTRRFHVVTYDARGHGHSAKPASGYGFDTVVADALAVLRATRLRRALMVGHSWG
                     ASVALELAARHPRSVGGAVLVDGGLGALRDAMDWATVKEVLAPPRLAGMPVEEFRGSI
                     RTFLGDDVEITPQIEDIALSLMRVDRRGRIRPHLSRANHFRILRAIWLGHPDEALARL
                     SVPTLAVLARGGADADGEAHKRGSAERARRSGAPIRVSWLEGIHDLPLQHPAALAGRI
                     ERFSEGLVR"
     gene            11480..11743
                     /locus_tag="VF195_10480"
     CDS             11480..11743
                     /locus_tag="VF195_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581283.1"
                     /translation="MAWVILVLVLLAAAFGVLGAVIKAAAFLVLTILLAVAALVAIAW
                     YSFKGQLRRWERDGIRGDDTQVRTWSTRRSEPPRDLPSHDDRY"
     gene            complement(11759..12139)
                     /locus_tag="VF195_10485"
     CDS             complement(11759..12139)
                     /locus_tag="VF195_10485"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HEX6581284.1"
                     /translation="MRKLLPGERGTVWYALADATALIVFVLVGVSRHRADTLEGFLRN
                     AIPLLAVWFLVAWLARTYRRPGWRSLVRSWIVAVPVGLLLRTLIVGSPNGVRILVFIA
                     VGLVFTLVFLVLGRLLVRLLSRAR"
     gene            complement(12151..13050)
                     /locus_tag="VF195_10490"
     CDS             complement(12151..13050)
                     /locus_tag="VF195_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018539197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03620 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HEX6581285.1"
                     /translation="MGTEHEAVRARLGRVGVWSFAFDAIPAAAVRRAAADIERLGFPA
                     IWVPEGGTSREIFAHLSLLLSSTERITACSGIANVTARHPEAMAGGARTLADAYGDRV
                     VMGIGIGHQYTAGRRGQEWADPVGRMKAYLDAMDAARTGPPPEVPVRRVLAALGPRML
                     AAAAERALGAHTYFVPVEHTVQARTLLGPEPVLAVEQTAIVSSDPATVRGIARPWAAD
                     YLELPNYANNWRRLGYADDLGDGGSDRLIDAAFAWGDVSSIAGRVREHLDAGADHVCI
                     QVISGRDDDVCLPALRELAGVLL"
     gene            13094..13768
                     /locus_tag="VF195_10495"
     CDS             13094..13768
                     /locus_tag="VF195_10495"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEX6581286.1"
                     /translation="MEVHAVIAELRDRADPSRKPGMARVGIDISKALGLGVPDIRAIA
                     KRCGTDQSLALELWRTGIHEARILATLVADPGALTSDHMEAWVADIGSWDVCDFAADL
                     FGRTSFGSAAIRTWAERPEGFVKRCAFSMIARRAVWAKEAPDREFLRYLPLIRRASVD
                     PRNEVKKGVSWALRQIGKRNRALHSAATEEATRILLLDAPSARWIARDVLRELEGEKT
                     RSRLPD"
     gene            complement(13777..16251)
                     /locus_tag="VF195_10500"
     CDS             complement(13777..16251)
                     /locus_tag="VF195_10500"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX6581287.1"
                     /translation="MRINPEALARASSRHPWRTLVIWVLVIAASVAVMVPLLGGVLNN
                     DIEFTNRPESVRAQEVIDERFATETAGVGTEYVIVSSEDLRIDDPEYRSFVEELRGAL
                     AAEKSLVAEPPATYYDVVAQSEEAAAGLVSEDRTSTLIPIAIADDEAEDVEDLRALLA
                     DHERAGFTTLMAGQATVFADFSEIAEEDSRKGESIGVATALIVLVVVFGAIVAALVPI
                     AMAIAAIALSLGLVAVIGQVVEFNLFVVNMISMIGLAVGIDYSLFIVSRYREERKKGY
                     PKLDAIGAAGATASRAVFFSGATVVLALLGMMILPTTIFRSLASGAIFVTVMSLLAAL
                     TLLPALLSLLGDRTDWPWMSRLASVVAFLGILLVSGVVGGVLGAVGAPPYVVLVGVVA
                     AIVGSAFLITRLVRRSSSRIVQRVRRDIGGDHDPRGGFWDRLTRGVMKRPIVFLLASV
                     AVLGTLGVFTFQLNKGSSTSIETFPDDVPSKEAFLTLEREFAGGLTEPAEIIVTGDVG
                     TPEVDAAVADLRERVAEMPQFAAQTQVAPSPDGTAIRVSAFFQGTTQNDAAFGAITEL
                     RDDVVPSVFSDIPGVEVFVGGNTAFFSDFLDLTDTYQWIVLAFVLGLSFILLMVVFRS
                     IVVPIKAIIMNLLSVAAAYGAITLVFQKGVGIDIFNAIGFSFQQTEAIEAWLPLFLFS
                     VLFGLSMDYHVFLLSRIREEYDKTHDNTEAVAYGLRTTAGIITGAAIIMVTVFTGFAS
                     GRLGPFQQMGFGLAVAVFMDATIVRTLLVPASMRLLGDLNWYLPKWLQWLPKLNVEGH
                     EPERVVVPDTPAELVEAGEPRGPDLG"
     gene            16394..17053
                     /locus_tag="VF195_10505"
     CDS             16394..17053
                     /locus_tag="VF195_10505"
                     /inference="COORDINATES: protein motif:HMM:NF014906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEX6581288.1"
                     /translation="MNETRSREPLTRDRVIEAALRVMDEEGLEAVSMRRVAREVGVEA
                     MSLYHHVEDKEDLLDGICEHVMAGFEFPDPVGDWAENCRRGARAWRRLLQSHPAVMRL
                     FADQRGPVRTVDSMRPMEFALRLLRGAGLSDRDTAQAFHAFGGYIQGFVMMELGSIAG
                     GSDEAHLKAHADLVSALPEEFEALVAVSPYFAECDADEQFEFGLDLLIRGLEAKAART
                     D"
     gene            17119..17973
                     /locus_tag="VF195_10510"
     CDS             17119..17973
                     /locus_tag="VF195_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581289.1"
                     /translation="MMVRLPAALALLLTLAACADSPVDPGVGGIDHSTAPDEVLVRIS
                     YEGGFVPVEWTYTNFAFYSLLGDGTLIVPGAQIEIYPGPALPAVSSRHVSEAGIQAIL
                     EQAMAVIRNIPADLNDLGFMNIADASTTVITVSAGGVDRTIRAYALSELTERPDGMPA
                     REYQARLSLQELVATLTGPTEWLPEGSLGPEGAYEASAARVFVGNYRAVDDLKQEPMP
                     WPLGAGLADFGDAIPPGGMRCGVVGGENWDTLRESAAAANQLTPWTDAGDRYSILFRP
                     LLPDERGC"
     gene            18080..20302
                     /locus_tag="VF195_10515"
     CDS             18080..20302
                     /locus_tag="VF195_10515"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6581290.1"
                     /translation="MTTEIEGSAVDPPRTTDGFFEPPAVGSADRTRELELRYRGIIDR
                     LPAVLYVDAVDQDQPMIDVSPSVHDLLGIPREEFLSRPYAWTDTIHPDDLAAVMAENE
                     RSAVSGDPFRMEFRVVHPDGRILWVRDDAVLIHDGSGAPLHWLGLMLDVTELIEAQAE
                     LHQAKTKYGALVEQIPAIVYVDAADDEMSTTYVSPQIRAILGYTAQEYIDDPQLWERM
                     LHPDDRENAVATYLRGRESGGPFVFEYRLIAHDGRTVWFRDSAIVLTDEQGRPEHIQG
                     VMLDITERKNAEERIAFLAYHDKLTGLPNRTMFDELLGLALARAKRHELGVVVVTVDL
                     DDFKLVNDSLGHEAGDAILLQFAQRLSDATRETDLVARPGGDEFLLLLADLDRESSGH
                     GGHESVSVSAESVALRIQEALRTPFAVGGTELYVTASLGISAFPDDAVDAPTLLKNAE
                     TAMFRSKRSGPGGYVLHAKDDADSIVRLSLSTRLRRAVEQEQWMLHYQPLIDLRTSEM
                     NGVEALIRWPEPNGGLVPPGDFIPLAEEMGLIEAIGAWVIDEVCRQGRLWREQGLDLD
                     ISFNLSPRQLWQNDIVDLIVDPILGSGMDPSRVTVEITESTAMTNPDRTLGILHELHA
                     RGLQLAIDDFGTGYSSLARLRYMPVDVLKIDRSFVRELDRDEQNASMVSAMIALASNL
                     GMTPLAEGIETEAEWRALTQRGCELGQGYFFSRPVPAAEILAIHRRAELHVVEGGAAG
                     "
     gene            complement(20324..21526)
                     /locus_tag="VF195_10520"
     CDS             complement(20324..21526)
                     /locus_tag="VF195_10520"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6581291.1"
                     /translation="MHRTMITFRDLGVSEAVVRALEARGIESPFPIQVMVIEDATSGR
                     DTLARSKTGSGKTLGFAIPIVDQIDPGVERRPAALVLTPTRELAQQVSDEFEDIARAR
                     KLRVMAVYGGTKVREQSKGVTSAHILIATPGRLDDLVERGLVKLDGVRIFVLDEADRM
                     LDMGFQPQVDRIVRHLPKDRQTMFFSATLDGAIGRIAEVYTRNPMRHEVGSVEMRFVE
                     EADHRFIPVAPQDKLSTLARLASEASGSTLVFVNTKRAVDHLARQLRAEGVTVVAMHG
                     DLTQQARERALGRFEDGHVGILVATDVAARGLDLERITLVVNYDPPKDDKGYVHRVGR
                     TARAGRSGTSITFVTPEQLGDVSRMAARLDLHAEFEQEGMKVSPPRVVFSGGARGKRS
                     GLRPRRRR"
     gene            21638..23494
                     /locus_tag="VF195_10525"
     CDS             21638..23494
                     /locus_tag="VF195_10525"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011318468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX6581292.1"
                     /translation="MGDRNGTDDRVGLWTELAAQLAVDSIRCTTAAGSGHPTSSMSAA
                     HLAAVLFADHLRTDLADPHGAGNDRFVLSKGHAAPLLYAVLKAIGAIDDDMLLSLRRL
                     GSPIQGHPAPVPELPWVEAATGSLGQGPAIGLGMALGMRLWGSPARVWVLVGDSEAAE
                     GSIWEAVEAAAHHHVSNLACVLDLNRLGQRGPTMHGWDADVFVARAQAYGWHALSIDG
                     HDVTAIDAAYRSAAGADRPTMIVAGTEKGHGVSEVANRDGWHGKAFPEDLAARAIDEL
                     GGVRSLTITPQKPEPWMPAGRVAPTGAPAPTYAEPIATRKAFGETLRWLAGERDDLVV
                     LDGEVGNSTYTEDAEAVAPDRFFQMYIAEQTLVGAQTGLHALGATAFSATFGAFLTRA
                     YDQIRMAAIGRADLRLCGSHAGVSIGEDGPSQMALEDLAALRAIHGTTVLYPSDGNAT
                     VGLVTSMADLRGISYLRTTREATPVLYGPEEDFPIGGSKTLREGDDVTLVGAGITVHE
                     SLEAADRLAADGVEARVLDCYSVKPIDAESLRNAAAATGLLVVVEDHRIEGGLGDAVL
                     DALAAGGTLAGRVVKLAVTDMPGSGAPEELRGWAGIDADAIVGAVRSALGRD"
     gene            complement(23502..25757)
                     /locus_tag="VF195_10530"
     CDS             complement(23502..25757)
                     /locus_tag="VF195_10530"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX6581293.1"
                     /translation="MDVSQGSSSAAVPAVDPGVSSAASPLAQVSDALANVGRELGADR
                     VALFENIRDPDGRLWMDLTSEWRRNGVRGIFDDPSTTLHPYSPDFIRWIEVLGDHRVL
                     SASVSDLPESERRILAAEGTVSFVAVPIVVDEGWWGYLTVDDCSGGRSWGTGEIELLR
                     VLARSIGADIQRQRQASADEIVGDRYRSIVEHIPAITYIDALDDAASTIYMSPQVEQL
                     LGYAPEEWIGDPDLWLRIVHPDDRPRAIAENQRHNETGESFALDYRMYRKDGRIIWIR
                     DEARIVRDERGIPAFSLGVMFDVTEMKRGHEKVAFLTYHDELTGLPTRAMFEELLELS
                     IARAKHHEDSVAVICVDIDDFRLVNDSLGRLAGDEVLNIVAERLREATRETDLVARRS
                     GDTFLLLLADLERDGDDVEAAVARAESSAQRILDSLTVPFRAGGTELYVSASIGISLF
                     PQDGDEPGSLLRNADAAMLESKKGGHAYVVSNRGALDSSAKLRFVTRLRKAVESQRWT
                     LHYQPVLDLTTGRMRGVEALIRWIEADGTMLLPGDFIPVAEELGLIEAIGDWVVRELV
                     YQAQAWRELEMDLSISFNLSPRQFWQPDLASGILDRIREGGIDPAKVIVEITESSAMT
                     DPDRAQMILEALHDGGLRIAIDDFGTGYSSLARIRDMPVDVLKIDRSFVSGVDVDPQK
                     QSIVTAFIELARGLGVTTLAEGIETEGELAFVRERGCVMGQGFHFSKAVPPEEIIAMS
                     FGGLHVASEAS"
     gene            25834..26115
                     /locus_tag="VF195_10535"
     CDS             25834..26115
                     /locus_tag="VF195_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581294.1"
                     /translation="MADYKQVLHERLYVRVPGTDKLRKTAAARLKHLVATGWRETERD
                     RKPDHIWVRFERTGHAPMRARLPKKAPEIQIERRPRRDNRGGGFRGGRR"
     gene            26249..27676
                     /locus_tag="VF195_10540"
     CDS             26249..27676
                     /locus_tag="VF195_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015350698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX6581295.1"
                     /translation="MEFLSPPSGRPGHRDPHAHAAAQPFSYPLQRPYAEPDWTRLDAY
                     RDVTTEQWESAQWQRAHTVKNLKEFKAALGGHLDDELLADIERDQQERATMSMLLPPQ
                     MINTMDETDLRADPVRLYMAPAFSDRDQEFPSHPFASRDSLHEADMWAVEGLTHRYPT
                     KVLAELIPTCPQYCGHCTRMDLVGNDTVQVLKYKFEAKQNDRWEAMLDYLRRTPSVRD
                     VVVSGGDMANVPIKRLEAFVMGLLDIENVRDIRLATKGLMGIPQHFLQDEVRAAAERM
                     ASRASGRGVDIAVHTHVNAAQQVTPLVGRATKVFLDAGFRDVRNQGVLLRGVNTAPQQ
                     LLELCFMLLDHAKILPYYFYICDMIPNAEHWRTAVWEVQDLQHAIMGYLPGFATPRMV
                     VDVPFVGKRWAHQVAEYDRVRGISGWTKNYRTGIESDDPEAFTRRYHYYDPISTLPED
                     GKAWWREQLARGVDELIEELQPQHT"
     gene            27750..28604
                     /locus_tag="VF195_10545"
     CDS             27750..28604
                     /locus_tag="VF195_10545"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6581296.1"
                     /translation="MSRVLTVLSAQVAPVAWDPAATLDKFEDHVRVARRAFPDVDLMI
                     FPELYLTAIDGFTSGGSADWERRVAEEIPGPLTDRVAKIAARAKRWIVAGSINERHGK
                     KIHNTAIAFSPDGEIAAVYRKLFPWRPFENADAGAEPAPVFAIPGKGVVGMMICYDGW
                     FPEVARGLALQGAELIAQPTATTTPDREEEVVLARANAIVNQVFMFNPNMVSTFGPGR
                     SVGVDPEGRVLFEAGAGEELLVEVVDMDRVAEIRRRGTRGVSRPWEHFLEGPRDVVLR
                     PYRARRTT"
     gene            complement(28580..29275)
                     /locus_tag="VF195_10550"
     CDS             complement(28580..29275)
                     /locus_tag="VF195_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581297.1"
                     /translation="MSRAGPARRAAVIAIVPLLVAASDPTGDVAGCGAPPGPGTPPDL
                     VDARGEIVELGTSARWTLTFAEPLTVPDAVGRPFRVDIAIRDPDVPPLSFAYYTDVNR
                     LVRVDATVAHETEIYLLPERGANVFNPPIIEGATMTIQIPGRTLSADEDLTGTSPGLE
                     ALRWTVIVRDGRACDVLGDGKPTERFDVGIEPVGDDEPAGDTRSGLSMWWLVTGAAVA
                     TLGIAGYVVRRAR"
     gene            complement(29275..30702)
                     /locus_tag="VF195_10555"
     CDS             complement(29275..30702)
                     /locus_tag="VF195_10555"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6581298.1"
                     /translation="MRRARVRQRRQTFGALVVVLTSILAWQFWPSAGQERGETPDPGD
                     DGNGIQSPSPDPNSPIQHVIFLVKENRSFDHYFGTYPGAEGATEGGTIEDCDATAFGD
                     GPVIDLEEAPLVLPHDLGHAFAPGLYSINGGKMNGFNCVSLGDDLMGYLQHSRETLPA
                     YWAYADRFVLADQFFTSMYGPTFPEHLYTVAAQSYGIVDNKTTTNVEGNYCDDDTEIT
                     KRFPIEDLTDADVERIMALEEDITSDVPGQLIRIAAYWESTRTCINIPVLPDKLERKG
                     ISWRYYANADSWMNALQAVEHVRSGPMWDKVVPPETFLQDVRRGELPAVSWLIPPEGN
                     PNEHPGSMTNVCEGENWTVEYLNAVMRSDAWPNTAIVIVWDDFGGFYDHVVPPHYDIM
                     GLGPRTPALIISPWTKAGENPDGGSIDSTVYEFSSVLRFIEDLHGLKPMTDRDRQADP
                     LSGAFDFEQEPRLDPLIIEPRDCPS"
     gene            complement(30739..32121)
                     /locus_tag="VF195_10560"
     CDS             complement(30739..32121)
                     /locus_tag="VF195_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX6581299.1"
                     /translation="MRLAPPGRRMAGAALVALGGLGAALLAPQPDGRSDDRPNVIVVL
                     TDDQRAGSFAAMPWLASQLARPGTDWAGFPNAFANTPLCCPARASLLTGLFARHTGVL
                     DNGDGARFDASSTLATWLHDEGYRTGLVGKYLNRYPFGRLPFVPAGWDRFLAKQNQIA
                     ATVYRDFHAVDQGSPVFVRRYATDWLADRAVGFVRSAPPSQPFFLLFAPSAPHHPWIP
                     AARHDGILAGLAIEEPPSVAGTLRGAPPWVRSLPAPSDAQRTAWLEEQRRADETLLAV
                     DEALHALVSALGDRLDETVIFVLSDNGYSFGEHRWEGKTCPYEACVRVPLAVHASGLR
                     PGEREELVSMVDLAPTILDLAGVRAPATMDGTSFAGRIGAGDVTGDPGDAVFLEWAGD
                     ERIPAWTAVRTSDFKLIRYAGGFEELYDLSGRRWPADPWETTNRATDPRAAGLLGRLR
                     ALLGRVLGPG"
     gene            complement(32118..32897)
                     /locus_tag="VF195_10565"
     CDS             complement(32118..32897)
                     /locus_tag="VF195_10565"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6581300.1"
                     /translation="MTQDTNEHVRKNREHWEREADEYQERNRAQLNRWDRLGWGTYDI
                     PEDEIHALGDVRGRRVLEFGCGGCQSGIKVAMRGANVSGLDLSFAQLRHGMAHFEETG
                     VRFPIVQASGECVPFRDGSFDLVFCDHGVMGFADPFLTVPEVARVLRPGGLFVFNGTT
                     PWIWVAWGDQDEELPTTREMRADYFEMRSIESDDPSWRTTEFQLTYGDWIRLFRANGL
                     VIEDLIELRPDSDATTTYDDYAPLEWARAFPGEHIWKVRKE"
     gene            complement(32894..33685)
                     /locus_tag="VF195_10570"
     CDS             complement(32894..33685)
                     /locus_tag="VF195_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX6581301.1"
                     /translation="MELLGGATGPHPSIPDQHATAPAATATSIEERAPGPWVDVKAVR
                     VIGSPPCAVGNAMAIVPGGEVRGTLGCAEFDAAAVTAAAEVYAAGAPEMRTLHHPEGD
                     VEVYLEPHRAPPRLVVVSATDVARSLRVEMERQGRRVIVLEPRAERVAPDDGPSVGSL
                     EELTLGYDDEVVFTDHDAPGIADMLTALLRSQVRFIGVMGSRRHVAHYVEDLRSRGFG
                     EVDLRRIRSPLGLDLGGRRPEEIALAIAAGLVAADHDREGGWLDR"
     gene            complement(33668..34138)
                     /locus_tag="VF195_10575"
                     /pseudo
     CDS             complement(33668..34138)
                     /locus_tag="VF195_10575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267440.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
     gene            34259..34504
                     /locus_tag="VF195_10580"
     CDS             34259..34504
                     /locus_tag="VF195_10580"
                     /inference="COORDINATES: protein motif:HMM:NF040429.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6457 domain-containing protein"
                     /protein_id="HEX6581302.1"
                     /translation="MEWVDRLAAELKLDVLSPHEKDHLLTASREVAHRVERKATPLAM
                     YVIGLSVGSQLSDRARDDSIEDAIHALLLRLPPADES"
     gene            34539..35564
                     /gene="moaA"
                     /locus_tag="VF195_10585"
     CDS             34539..35564
                     /gene="moaA"
                     /locus_tag="VF195_10585"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEX6581303.1"
                     /translation="MTDRIPHEAPMPLDGPLVDTYGRVADDLRISVTDRCNFRCTYCM
                     PAEGLAWLPKHEILTFEELTRVLGIFVRLGVRSLKVTGGEPLVRADLPVLIRMFREAG
                     PDLDISLTTNGVLLDRLAGPLAEAGVDRATISCDSLLRHRFEEMTRRDALDKVLAGMR
                     AAEAAGLTPIKINCVVIGGTNDDEVVDFASWSRETGFEVRFIEYMPLDAQHAWEREKV
                     VPAARILAAIDTVHPLLPEGHANEPATTFRFADGAPGRVGVIASVTEPFCDTCNRLRL
                     TAEGMFRNCLFAMGETDLRAPMRGGASDDDLALLIRQGLWAKWSGHRINHPDFVQPAR
                     SMSMIGG"
     gene            complement(35702..37642)
                     /locus_tag="VF195_10590"
     CDS             complement(35702..37642)
                     /locus_tag="VF195_10590"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015143569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX6581304.1"
                     /translation="MTVAAPFGTWRSPITAERVSAGAVSLMQPHLHEGVAYWLEGRPA
                     EGGRSVLVRADVFSEPTDVTPPGFNVRTTVHEYGGGAYLLHDDTVYFSNFADQRMYRQ
                     DAGGDPVPITPDTGGRDRYADGRVTPDGRSLVCVRERHPEPDDQAGVVNELVTLSPDG
                     SSEPRTIRSGRDFYSTPRIAPDGSRLSWLEWDLPWMPWDGCELVVGELARDGSLSGVR
                     RVAGLVGEESIFQPSWSPNGDLHFASDRTGWWNLSVERDGDVQGLHPADAEFGWPQWV
                     FGMSAYGFLSDGRIACLWERDGLQHLAILDPLSGELIDLDVPYTAMAPRLDVEGDRVV
                     FVGGGPATPDEIVVLDVTARSIDVLRSSSSVVDEAAISIPRQIEFPTEGGLTAFAHFY
                     PPHAPGAVGPEGERPPVIVMSHGGPTAEATPRCSLETQFWTSRGFAVVDVNYGGSTGF
                     GRAYRQRLQGMWGIVDTADCINAAAWLASRGEVDGDRLLIRGGSAGGYTTLCALTMHE
                     GFAAGTSYFGLADLESFVSGGTHKFESAYLLSLVGPYPAEAERYRARSPIHATDGISC
                     PMLLLQGDQDRVVPPHQSEIMVEALRRKGLPYAYVLFEGEQHGFRKAESTRIALEAEL
                     SFYAQVLGFDRDDVPLLPIENL"
     gene            complement(37639..38499)
                     /locus_tag="VF195_10595"
     CDS             complement(37639..38499)
                     /locus_tag="VF195_10595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6581305.1"
                     /translation="MKARTVLDDAVKVLKASPAIDHWQRGRERIEAEDLLWHVLGEEP
                     DPRDDVTVEQRRTLERLVARRATGEPIPLIKGSTEFRGLDLIVRPGVFVPRDASEFLA
                     EQAMRRLRGRPRPVHLDLATGAGTIALAVANEVRGADVIGTDLSADAVKLARTNARRL
                     GVRARFFTGDLFGGLPRTLEGTVDVVTLHPPYVPMHELVDLPEEIREWEPEHTLTDRS
                     PDGLGLISRSAREAPTWLSANGWLLLEVSPDRVRHVKGVLRGEGFRDIRSTKGGPLPL
                     TRVIVARRPR"
     gene            38542..38952
                     /locus_tag="VF195_10600"
     CDS             38542..38952
                     /locus_tag="VF195_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012086503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX6581306.1"
                     /translation="MPGTTPADGAKSTRTAKPRRARARKSPSTGEDLRLTGDRLVVQL
                     PENGERKSASGLLIPATAAPAPKRLAWADVKLVGPDVRVAAQGDRVLFLPSSGLEVEL
                     DGQELVLLRERDVQAVSSPPASDARSQRAPGQYL"
     gene            complement(38981..39385)
                     /locus_tag="VF195_10605"
     CDS             complement(38981..39385)
                     /locus_tag="VF195_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003911902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEX6581307.1"
                     /translation="MRTTEFELSTKGRRVVDLTDRVRAYAGASGGDGLLHVFLPHATA
                     GLALMETGSGSEEDLDAALERLLPRDDRYTHRHGSLGHGGDHLLPVFVTPTLTLLVED
                     GRLVLGTWQSIVLLDPNRENDIRRVRLTFIDG"
     gene            39488..39997
                     /locus_tag="VF195_10610"
     CDS             39488..39997
                     /locus_tag="VF195_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020545144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581308.1"
                     /translation="MEEELSGWFAGRIPDDWFQGAPTVTADREEIYVVGTLAEPELPA
                     DAGEEAREAALTARISGFREDTRRARMRIADEAQRRFGRKVSWGASCGDLTAAFTTLS
                     VPAMTRLRMEERRVLDTLIEAGVARSRSHALAWCVRSVAERQDDWLKDLRGALEKVRE
                     VRSGGPTTG"
     gene            40052..41359
                     /locus_tag="VF195_10615"
     CDS             40052..41359
                     /locus_tag="VF195_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEX6581309.1"
                     /translation="MAERGRGVRRRSTSIALLTLLLSVVQLLGATTANATPPSWTLGE
                     DGSGPPPLSTHLSRSERALIPTSTWSDLEPDDAWAKVAINHVAGTHNWMRDFALDQNG
                     NRRFRPDAFESRARWARAMARAFAPGEEPDPSITFTDLDPSDPFHRSAAIAVQHGWMT
                     RGPGGRFNPTSPVSARGVHRSLVRALGMGQTAKALDRLSTSDGYRFATPRSFGVDLLA
                     MRLGLRFNNKVDESQDVTPGSLLRRKQVAWSLYRATTLEDWVVGYLEDQYADMVLPKM
                     GVGRRSIVDWGVRYVGYPYIWAGEWGFESPSPPAFGGQPGPGFDCSGLTWWALRADDG
                     VYWEISPPRPHEGWALPQRTSAEMARMTKVKLRYADLLPGDLMFYDGNRDGVVDHVDV
                     FVGNGWSLDSSSSVGGVTLMWVKSGWYRDHFVHGRRVLPTPKP"
     gene            complement(41356..41769)
                     /locus_tag="VF195_10620"
     CDS             complement(41356..41769)
                     /locus_tag="VF195_10620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011028505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX6581310.1"
                     /translation="MTTVTAEVRIPIRWRDVDAYGHVNNAVFLNYLEEARDRLVESLF
                     GAEAWDFVVARVAIDFRSELQQRDREVIVRCGITSFGTSSIRTGELVHAADGRLSAEA
                     ESVIVARDPEAGRSRQLTEAERNTLQSAIDANGAV"
BASE COUNT         6683 a        14729 c        14185 g         6440 t
ORIGIN      
        1 tcggcgccgg ccggcggcca cggctaccag gtgcacatcg gaccgatgta ctccgacgcc
       61 cgcggctccg tgaaggtcgt gagcaccgac ccgcgggtcc accccgcgat ccggttcaac
      121 tacctgtcca ccgaccagga ccgccgcgaa tgggtcgagg cgatccgcgt ggcgagatcg
      181 atcctgtccc agtcggcgtt cggaccgttc gacggcggcg agctctcccc cgggccggag
      241 gtggcgacgg acgagcaggt cctggactgg gtccggagcg acggcgagac cgccctgcac
      301 ccctcctgca cgtgtcgcat gggaacggac gaccggtcgg tggtggatcc ccggacgatg
      361 cgcgtccacg gcgtggagaa cctccgcgtg gtggatgcct ccgcgatgcc ctacgtgacg
      421 aacggcaaca tctacgcccc cgtgatgatg ctcgccgaga aggcctccga cctgatcgcc
      481 ggcaacacgc cgctcgagcc ggaacacctg gagttctacc gccacccgga ctgatcgccg
      541 cggctcggcg aacgacctgc atcaaccgag gccgaggagg agcagaccgc ccaccaccag
      601 gacgatccct acgatctggt tcgcgacgag ccgctccttg aagacgatca ccgacagcgc
      661 gaccgcgatc aacgggaaca cggcgctcga ggcgagcacg atggagttca ggccgcgctc
      721 ggagccgacg gagaacgtca ccacacccag gatgtcggcg gcgccggcga ggagcgcgat
      781 ccacaggccg aaccccggcc gcagccgaga cagctccctc ctgaacacga gcacgagggg
      841 caggaagaag acgatctggg cgacgcgtga gccccagagc cccgcgaccc atccggcccg
      901 gtctgcgatc cagcccagca ggaagcctgc gaccccgaac ccgacggcgg ccgccaccga
      961 ccagggcaga ccgggaagat ggttcttgat cccgtcccgg agcttgcgga gatccgtcga
     1021 gacgagcgcc accccggcca ccgcgatccc cgcgccgatc aaggcgaggc cggttgggcg
     1081 ttcccccagg atgacgatcg cgaggacgac gcccaccacg gcgtagcccg caccgatcgg
     1141 cgagaccacg gccacgggac cgagctccag cgccctgtag tgcgtcaggt acgcgaacgc
     1201 cgcggccgcg ccgttcagca ggacgagcca gacgtccggt ccgagtgggg ccaacgacgt
     1261 gcccgtcgcc acgagcaccg cggtcatgaa cgccgcggac aacacctggc cgctcatcac
     1321 ggcgccgatc gagccgatcc tccggcccga gaccgccccg aagaagtcgg cgagtcccca
     1381 tcccagggcc gcggcgagcc cgaagatcac gccggtggcc gcatcaggcg tcgccgcgga
     1441 acctggcgtc gcgccatgcg atggccgcct tcagtccctc ctccgccgtg atgcgacgga
     1501 actcgtctcg ggccggcagg ttcgcagtct cgatcatcac gtcgagctcg gtgttcgcgg
     1561 ccagggcggc ccggaacccc gccacgtccc acgcacgatt gaccgcgcgc ttcgtggtct
     1621 ggatcacgaa cggctcgttc ttcgcgatct cctccgcgag ctgcagggtg gcggcgtcga
     1681 gctcctgacg ggggacgacc cggttcacca agcccagccg gagcgcctcg tcggcggaga
     1741 cgcgatcctc ccccgtgaag agcagctcct tcgcggcccg gacgcccacg acccagggga
     1801 ggaacatcga gacgacgccc gatccgaacc ggacgtccac gtagccgaag taggcgtcgt
     1861 cggccacgat cgcgaggtcg cacgcgagca tcagatccgt cccaccggcg aggcaatagg
     1921 agtggacgct cgcgatcacc ggcttcgggt ggtccaggaa cttcaccatc tcctcggtcg
     1981 agtgctgcag gtccgcgtgc cagcgacgcg cgtcgagcac gcctccggcg gcgtcctcgt
     2041 tcaggtcgta gccggcgctg aaggcccggc ctgccccccg gaggatcagc acccggacgt
     2101 cctcgtccgc ggcggcctcg tcgagcgcgc tcgacatcgc atccacgagc tcccggtcga
     2161 gggcgttcag cgaggcaggg cggttcatcg tgaagcggcg gatcggaccc aggtcctcca
     2221 ccagcagtac cggctcgctc acgcagtccc ccctgcccga tgaccgccgg atgctagcgg
     2281 agacgcgccc ccgccgggca acccgcgacc ccccggcgga cgtaatcctt gaggaggagg
     2341 ctccgcccgg ctgccttgcg ctgctcgcga aggctggggt agcctcctga accggcgttc
     2401 aatcatcgat cgagggaggc gtcatggtct atcacccgag ggatcccgcc catcgcagcg
     2461 tctcgcgtcg ggaattcctc cgccgatcgc tggccgcagg cgtggccctg ccgacgtcgg
     2521 ccgcgatcct cgccgcttgc ggcgaggacg ggaacggcac gcccgatccc ggcgccgagg
     2581 atcagatccc ccgcttcggc accctcgaca acccggtcac cctgcagacc tacgacgaca
     2641 acccgccgat cgactcgggc ctcgagccgg aggccgggcc cctggtgatc tacaactggg
     2701 agcagtactt ctggaagcag gtcctgaacg acttcagtaa gaaatacgac gtggaggtca
     2761 agtacgagac cttctacaac atggagcagg cgatccagaa attccagtcg ggcgaggtcg
     2821 actacgacgt gttcttcccc acgatcgact acgtcccgaa gctcgtggcc gccaagctcc
     2881 tccagccgct caaccacgac tacctgccgg acacgaagaa cctgtggccg gtgatgcagg
     2941 acccgtatta cgaccagggg tcgctgtaca cgctgccgta cacgatctat cacaccggca
     3001 tggcgtggcg gaccgacctc gtgccggtgg agccggagga cctggcggcc tccgggaatc
     3061 cgtacgagat cttctggaac gcggactacg caggcaaggt cgggatctac gacgcctacc
     3121 gcgaggcact gtccctgggg atgtatcgcg atcatctcga cgagacggac ctcaatccgg
     3181 gggacgctgc cgtgatcgaa gcggcgagag atcgattgat cgagctgaac gacctcgtcg
     3241 gggtcaagac gacgatcgac ggtgcctatg ccggcgtccc cgaagggaag ttcggcctcc
     3301 accagtcgtg gtcgggcgac atccaggcgg gcccgtggta cacgagcgat ccgaagaccg
     3361 aggcgcccct cctgcgctac tactggcccg cccgcgacgg gttcggcggc acgatcgaca
     3421 acgacacgta cgcgatcccg acgaacgcga agaaccctgt gctcgcccac cttttcctga
     3481 acttcatgat ggaggagttc aacgcgatca agaactggac ctgggtcggc taccaggttc
     3541 cgttcagcgc tgtcaacgtc gacacgatct tcgaggagag ctacccgggc ctcgaaggct
     3601 ggttctcgtg ggactacgga gctcccaagg gcgagcactg ggacaacctg aagtcgacga
     3661 tcgtgagcga agaagacttc gacatcggga agcgtgcgac ggggctgccg gtcgaggtcg
     3721 acgagctctg gtcgacggcg tacgcggact tcaaggcagg ataacctccc ggggatgtcc
     3781 actccggcgg gcggaaccgt cgggcagcgg cgctggtact accccgcgct cgcgatgccc
     3841 gggatcctct ggttgctgct cctgttcatc atgccgttct acgcgatcgc ggcggtcgcg
     3901 ttcggcaccc gtgaccagat cttcgccctc ccgatcccgg catggaaccc cttggagtgg
     3961 cagttcgaga cgttcagcga gaccgcgcgc gagctgttcg cgtccggggg tatccaggcg
     4021 cagttcacgc gcaccatgct ctacgtggcc accgcggtcg tgctctgcct gctggtcggc
     4081 tatccggtcg cctactacat cgcccgctac ggcgcgcgca tgaaggtcct ccttctcacc
     4141 ctgatgatcg cgccgttctg gatgagctac ctgatgcgga tgctcgcctg ggtgaacctg
     4201 ctggcggtga agccggacga tcccggtctg ttcaaccggt tcctcctcct cactcccttc
     4261 gtcaacgacc cgatcgcgtg gagcagcgga cgggtctccc acatgacggt gatcctcggg
     4321 ttggtctacg gctacatccc gttcttcatc ctgcccctct atgccgcgct cgatcggatc
     4381 gataagtccc agctggaggc gtctcgagac atgggagcga gcccggcgag cacgttctgg
     4441 cgggtcaccc tgccgctctc gaagcagggg atcctcgccg gagccgtgat catcaccctg
     4501 ccgatgttcg gcgactacta caccccgaac ctgatgaccg cgagccccga gaacacgatg
     4561 atcggcaacg cgatcgatct cgcgttgcag ggtgggcagg gcacgcagcc gaagggagca
     4621 ggtctcacgc tgctcctgtc ggcgttcatc ggcgctctga tgatctacta cctggtgagc
     4681 gtgtcgaggg ctgcgaaaga ggcgcaacga tgaccacggt cgaggcgacc gtgccgcgcg
     4741 ccaccgccgc ccccaaggtc ggctggctcc gctcgctctt ctccaacccg tgggggaagc
     4801 cacggttcct gtcggtctgg acatggctgt acatcatctg gacgatcgcg ccggtcgtga
     4861 tcgccgtgca gttctcgttc aacgcggggc gctcccgcag cacgttccag gggttcagca
     4921 tgcagtggta cgaggcgatc tggaacgagg catccgatgg cttcgacgac acgctcatgc
     4981 cggcgctcgt gcagagcgtc aagctggcgc tgctcacgat gccgatcgcc accgcgctcg
     5041 gggtgggcct ggcgatcggc ctggcccgat ggcgcggccg cggctccggc gcggcgaact
     5101 tcctgatgct cttcccgctg gtgacgcccg agatcgttat gggcgcctcg atgttcctga
     5161 tcttctcgaa cctgtaccac atccggttgg gcaccacggc gcagctgctc ggccacgtga
     5221 cgttctcgct gtcctacgtc gtgatcgtga cccgcgggcg gctgttcgcg atcgggaggg
     5281 actacgagga ggcggcgatg gatctcggcg cgtcgccggc gggcgctctc cgtcgcgtgc
     5341 tgctcccgct gctcgcgccg gcgatccttt cgagcttctt catcgtcttc gcgatctcga
     5401 tcgacgattt cgtgatcagc cagttcctct gcgcgcagga ctgcacgacg atcccgatca
     5461 agatctacag cgcggcgcgg aacgccggga acccgagcct gaacgcgctg gcgacgatca
     5521 tgatgctcct gtcgctggtt tcgatcacgg tcgccgtgct gatcctgcgg gtgttcaaca
     5581 agcgccaggg gacgggccgc tccgccgtcg aggacttcgc tcgattcgag atctgagcga
     5641 gctcagcgca tggcccgagc ggcggccgag aagccttcct cggacacctc accctgcggc
     5701 agcacgcgga gcgcctcgcg agggaggtgg acggagaccg ccgtgccctg ctcgaagggg
     5761 atcgcctcac cctcgttcgc cacgagcacc tggagcttct cgcccggcgc caggttgacg
     5821 atgacctgca tcgtggagcc cacgtagacg atgcgctcga ccatccccgg gatccggttg
     5881 gcgccggagc ctcccgaggg ctggagatcc acccgttcgg gtcggatcga cagcttcacc
     5941 tcgccgtggg cgtccggttc cccctggccg gcgacgagct cgaactctcc gaaccggacc
     6001 gtgcagccgc cgtccgtcgg tccgtaggcg gtggcgtcca tcaggttcga cacaccgagg
     6061 aagtccgcga cgtaggccgt ggacggctcc tcgtagacct cccgaggcgg tccgatctgc
     6121 tcgatccgcc cgtcggacat cacggcgagg cggtccgaca tcgtcagggc ctcttcctga
     6181 tcgtgggtca cgtagatgaa cgtgatcccg acctcctcct ggatcgcctt cagctcgatc
     6241 tggagtgcct tccggagctt cgcgtcgagg gccccgagcg gctcgtcgag gaggaggagc
     6301 gcggggttca ggatcaatgc tctcgcgagc gccacccgtt gctgctggcc acccgagagc
     6361 tggttggggc gacgcttctc gtagccggtc agccggacga gctccaggga ccggccgacc
     6421 atccgcgcga tctcgacctt cgaggtgtcc tggtaccgca ggccgaacgc gacgttcttc
     6481 tcgacgttca ggtgcgggaa gagggcgtag ttctggaaga ccgtgttcac gttccgcttg
     6541 tgcggcggaa ccgtcgccac gtccatgccg tcgaggacga cctgaccgtc cgtgggccgt
     6601 tcgaacccgg cgatcatccg gagggtggtg gtcttcccgc accccgacgg cccgaggagc
     6661 gagaagaact cgccggagtc gaccctcagg ttgatcccat cgaccgccgt gaagtccccg
     6721 aaccgcttca cgaggtcgac cagctggact tctccaccag gcatgagggc cggagtgtat
     6781 cgcggtgacc ggccgacgag gtcgttcccg agcgcgagcc gccctggcat catccgtgca
     6841 tggcgcgagc cgaggcggaa cgaggtgggg agccgctggt gcggagccgg acgctgcccc
     6901 tcctggcggg atactggagc ttcggacagt tctggggcgt ctgggtgatc ctcgtgctcg
     6961 aataccagcg tcagcacgcc ctcacggaca gccgcctcgg gctgatgtac tccctgctct
     7021 cgctggccgc gatcgggacg atgctggcgg gtgcccctcg cctgcagcgt ctgtcgctgc
     7081 gggccagcgt gccggcgtcg ctcgcgtgcc tcgcggtcgg atcgatcggg ctcgccgtgc
     7141 agcccacatc gctcgtgctc gtcacgttcg tggtcgtcgg tgtggggaac ggactgatcg
     7201 acgtgtatct caacgtcgcg gcgcaacggg aggagtcgcg gacgagccgg cccgtgctcc
     7261 agtggctcca cgcgagctac gcgctcgggg gcgtcaccgg ggcagccgcg gccggagtgc
     7321 tccggaccgt cggcctcgac caccggctcg ggctggcggc ggccggcgtc gccctgctcg
     7381 cgaccgccgg atggaacgcg cggcgagccg tcgacgagcc ggccgccgag gctaccgggg
     7441 cgctcctgtc catctcggca ctgcgacgga acccgacgct ctggatcccg gcgctcacgg
     7501 tgctcttctg cttcctcgtg gagggatcga tggacatctg gtccggtctc taccttcgcg
     7561 agcagctcgg cgcgtccgcg ggtgcggcgg gcgcggcgtt cgtggcgttc gcgggagcgc
     7621 tcttcgtcgg ccggctcttc gccgccaagg tcctcttcgg actcggcgcg cgtcgcacga
     7681 tcatcatctc cgggatcggc ggcgggatcg gcgggacgat cgccgtgctc tccgacacca
     7741 cggtggtcgt ggcggccgcc ttcctcctga tggggttcgc gatgtccgcc gcgggtccgg
     7801 cggggttcag cctcgtggag gggacgagcg aggacccgtc ggctgcgatc gccgccgtcg
     7861 cgacggtcgg ctacacgggc ttcgtctggt ccccgccact gttgggatgg atcgcggata
     7921 cggtcgacct ccgcgccgcg atgggggtga tcgtgttcgc caccgtgggg atcacgatca
     7981 tgggcttctt cgcgcggcag aagacggcgg agggaggggg cccggtcgag atatcccggt
     8041 gatcgctcgc ttgttgtgct tcggagcatt ctgttctatc gtcgcgcaac aggggtccta
     8101 tggcaccagc tgtgcgggtt gcgatcgccg tcgccgtgac actggggctc cttgcggggg
     8161 agggccttca tcctcagcgc gcacgagcgc ttccggctcg acccgacatc gtcgtgctcg
     8221 tcaccgacga ccaacacagc ggcacgctcg gatggatgcc cgtccttcgc agctacatcg
     8281 cccggcgtgg cgttcgcttc acgagagcga tggtgcccac atcggtgtgt tgtccttcac
     8341 gcgcctccat cctgacgggc gagtacgcac acacgacgaa ggtctgggag aacacccggg
     8401 gctgggcgcg cttccggcga gccgggatgg aagagcgaac gatggccatc tggctgcgtc
     8461 aggctggcta ccggacagcg ctcgtcggga agtacctcaa catctaccac cgggcccagc
     8521 caccgcccgg atggagcacc tggcacgcgt tcctcgagaa caacgcgaag tactacggct
     8581 acgagcttca gcacacgaac ggttccacca ccacgcatgg cttcgatgcc gcctcctatt
     8641 cgaccgatgt cctccgtcgt tacgcactcc gcttcatcaa ggcgacgccg agcgacacgc
     8701 cgctgttcct gtacctctcc ccggccgccc cccacgaacc ggcgactcct gcaccgcgcc
     8761 acctcgcgct tcccgcctcg atcgagtcgt tctggccgcc gagcgctcgg gagacagacc
     8821 tgagcgacaa gccgcggtgg atccgggacc tgccaccggt gagctccagc gccatccatg
     8881 actcccggat ccggcagtat cgctcgctcc gatccgtcga cgaagccatc gcagcgatca
     8941 tcgatctgca gcgggcgagg aggcgtttgc gcaacacgct catcctcgtc ctctccgaca
     9001 acggtgagat gtggggggag catcgagcga tggggaagtt ccttccgtat caaggggcga
     9061 ctcggatccc gatggcgatg gcgtggccgg caaccctgtc cggaggaagg gtggatacgc
     9121 gcctcgcctt gaacatcgat gtcgcagaga cggtggcggt ggctgcatcg tcccctcacc
     9181 ccgacgtcgc cggcaggaac ctcctccagc ggtgggagcg gagcggattc gtcctcgagg
     9241 ccagcggcgc tcaggccttc gggggaaacg gcacgaacgt aggacgccct ccgtactgcg
     9301 gctggcggac gcgtcggtac ctgttcgtcc actacggcaa cgggcgcgag gagctctacg
     9361 actacgcccg tgatccttgg gagcttcggg accgtcgagg taccgctccg gacgtgcaga
     9421 agaggctgcg acgacgagcc cgcgaagcgt gctcgccggt accccccggg ttcgcctggg
     9481 ggtagcgggg cgagcgaacg agacccactc accgtcggtc cactcggggt cgtcctcggg
     9541 gagcgcccgg aaccggccgt accacgcctc ggtggcgggg tcatgggcgt tcgccgagta
     9601 cgtcgtctgc tcctcgaaga tcactcccgc gaccagcgag ttctcgtcgt cgtccggcag
     9661 caggacgtac gtggcccgga atccctccgc gtcgaggctc tcgcccatca tcgccaccac
     9721 cgcctgccgg ttgccgggag tggccctcgc tcgcatcacc gttccgtaca tgtctcctcc
     9781 ttccctcgat ccaggtcact cgatgggcgg aggcgccgcc ccgatccccc aggcgtccgc
     9841 ccctccgtcg accgtgacgg tggaaccggt cacataggca ccgccgtcgc tcgcgaggaa
     9901 cgcgatcgtc gcggccacct cctccgctcg cccggcacgg cccatcggca cggacttcgc
     9961 gtactcgtcg acgagctcct gtccgccgta ctgcagcatc ccctcggtct ggatcagccc
    10021 ggccgcgacg cacacggcgc ggatcccgta cttcgaccac tcgatcgcga tcgtcgaggc
    10081 catgttctcg agcgcggcac gggctgccga cgagtgcgcc atcagcggga caccgcgcct
    10141 ggggctgaag ccaacgaagc acacgaatcc ccgacgggcg gggatcatcc aacgctcggc
    10201 gacgcggttc gtcaggtgcc atggtgcgtc gacgttcagc cgatgcacgg cccgcagccc
    10261 tttcaatccg atctgctcca acggggcggc gaactgcccg ccggcgttgt tgacgaggac
    10321 gtccaccgag ccgagccgtt cgccgacgac gtcgaggaag ccctcgacct gttccggctc
    10381 ccggacgtca cacggcatcg tgaccacctt gctgccggcg gcctcgagct cggtccgaac
    10441 ggcctcgagc ggttccggcc gccgcccgca gatcgcgacg ctcgcgccgg tacgggtcag
    10501 ctcccgcgcc gtggcgcgcc cgatgcccgt gccgccgccc gtcacgatcg cgacccggcc
    10561 ggacagggca tcgtcggcga gcggagaccg gaacttctct agcatgcgcg tccgagtatg
    10621 aaggacatcg acgtccgtgg agccggtgag atcaggctcg cggcgcgcgt ccacgacgcg
    10681 ccctccgccg gggcacgcgg gctcctcctc ctccacggac tcgcctcgtc acaacggatc
    10741 tgggacctga tgctcccccg tctcacgcgc aggttccacg tggtgacgta cgacgctcgc
    10801 ggacacgggc actcggcgaa gccggcgagc ggttatgggt tcgacacggt cgtcgccgac
    10861 gcgctcgccg tcctccgcgc gacccggctc cgccgggcgc tcatggtcgg tcactcgtgg
    10921 ggtgcgagcg tcgcgctcga gctcgccgcc cgtcatccgc gatcggtggg cggcgcggtc
    10981 ctcgtcgacg gcggcctcgg agccctccgc gacgcgatgg actgggccac cgtgaaggag
    11041 gtgctggcgc caccacggct cgccggtatg ccggtcgagg agttccgcgg ctcgatccgg
    11101 acgttcctcg gcgacgacgt cgagatcacc ccccagatcg aggacatcgc cctgtccttg
    11161 atgcgggtcg atcgccgggg gcgcatccgg ccgcacctgt cgagggcgaa ccatttccgg
    11221 atcctccgag cgatctggct cggccatccc gacgaggcac tcgcccgtct ctcggtcccc
    11281 acgttggccg tcctcgctcg tggaggggcc gacgcggacg gggaggcaca caagcgcggt
    11341 tccgcggagc gggcccgccg atccggcgcc ccgatccggg tcagttggct cgagggcatc
    11401 cacgacctgc ctctgcagca tcccgccgcc ctcgcgggca ggatcgaacg tttctccgaa
    11461 gggctcgtac gatgaccgtg tggcatgggt catcctcgtc ctcgtcctcc tggcggctgc
    11521 gttcggcgtc ctcggcgccg tcatcaaggc ggccgcgttc ctcgtcctga cgatcctgct
    11581 cgcggtggcg gcgctcgtcg cgatcgcctg gtactcgttc aagggccagc tccgtcgctg
    11641 ggagcgggac gggatccgtg gtgacgacac gcaggtccgg acgtggtcca ctcgccgatc
    11701 ggaacccccg cgggatcttc cgtcccacga cgaccgctac taggtcccga ggcggcactc
    11761 atcgcgcgcg cgacaggagc cgcacgagca ggcgaccgag gaccaggaac accagcgtga
    11821 agacgagccc cacggcgatg aacacgagga tcctcacgcc gttcggcgac ccgacgatca
    11881 gcgtccgaag gagaagtccc acgggaaccg cgacgatcca gctcctgacg aggctccgcc
    11941 atcccggtct ccgataggta cgcgcgagcc acgcgaccag gaaccagacc gcgaggagcg
    12001 gaatcgcgtt cctcaggaag ccctccaggg tgtcggcgcg atgcctgctg acgcccacga
    12061 gcacgaagac gatcagcgca gtggcatcgg cgagcgcgta ccagacggtt ccgcgctcgc
    12121 ccggcagcag cttcctcatg acatcgatgc tcagaggagg acgccggcga gctcccggag
    12181 ggccgggaga cagacgtcat catcacggcc ggagatcacc tggatgcaga catgatcggc
    12241 gcccgcgtcg aggtgctcgc ggacgcggcc ggcgatcgag gacacgtcgc cccatgcgaa
    12301 agcggcatcg atgagtcggt cgcttccacc gtcaccgagg tcatcggcgt atccgagccg
    12361 gcgccagttg ttcgcgtagt tcggaagctc gaggtagtcg gccgcccagg gtcgagcgat
    12421 cccacggacc gtggcgggat cgctcgagac gatggccgtc tgttcgacgg cgagcaccgg
    12481 ctcgggtccg aggagcgtcc gggcctgcac ggtgtgctcg acgggaacga agtacgtgtg
    12541 tgcgcccagg gcacgctctg ccgcggccgc gagcatccgc ggcccgaggg cggccagcac
    12601 gcgtcggacg gggacctccg gcggcggccc cgtccgcgcg gcgtccatcg cgtccaggta
    12661 ggccttcatc cgccccacgg ggtccgccca ctcctgcccg cgacgcccgg ccgtgtactg
    12721 gtgcccgatc ccgatgccca tgacgactcg gtcgccgtag gcgtcagcga gcgtcctcgc
    12781 cccgcccgcc atcgcctccg gatgccgcgc cgtgacgttc gcgatgccgg agcaggcggt
    12841 gatgcgctcg gtcgacgaca gcagcagcga gaggtgggcg aagatctcgc gcgatgtccc
    12901 accctcggga acccagatcg cggggaaccc caggcgctcg atgtcggccg ccgcccggcg
    12961 gaccgccgcg gccgggatgg cgtcgaacgc gaaggaccag accccgacac gcccgagccg
    13021 cgcgcgaacc gcttcgtgct ccgtgcccac gggctcgaac cctatcgtcg gcgggccgta
    13081 gggtctcggg cagatggagg tccatgcggt gatcgccgag ctccgagacc gagcggatcc
    13141 gtcccggaag ccgggcatgg cgcgggtcgg gatcgatatc tcgaaggcgc tcggactggg
    13201 cgttccggac atccgcgcga tcgcgaagcg atgcggtacg gatcagagcc tggcgctcga
    13261 gctgtggagg accggaatcc acgaagctcg catcctcgcg acgctggtgg ccgaccccgg
    13321 tgcgctgacg tccgatcaca tggaggcgtg ggtcgccgac atcggttcgt gggacgtgtg
    13381 cgacttcgct gcggacctct tcggccggac gtcgttcggc tccgccgcga tccggacatg
    13441 ggccgaacgt cccgagggct tcgtgaagcg ttgcgcgttc tcgatgatcg cgagacgagc
    13501 cgtgtgggcg aaagaggcgc cggatcgaga gttcctccgg tacctgccac tgatccgtcg
    13561 cgcgtccgtc gatccgagga acgaggtgaa gaagggggtg tcctgggcgc tccggcagat
    13621 cgggaagcgg aaccgggcac tgcactcggc cgcgaccgag gaggcaacgc ggatcctcct
    13681 cctcgacgcg ccgtcggcga gatggatcgc tcgcgacgtc ctccgggagc tcgagggcga
    13741 gaagacccgc tcccggctcc cggactgacg tctcgcctat cccaggtcgg gtccccgtgg
    13801 ctcgccggcc tcgacgagct cggccggcgt gtccggcacg acgacccgct ccggctcgtg
    13861 tccctcgacg ttcagcttcg gcagccactg cagccacttc ggcaggtacc agttcaggtc
    13921 tccgaggagc cgcatcgatg ccggcacgag cagggtccga acgatcgtgg cgtccatgaa
    13981 gaccgctacc gcgagaccga agcccatctg ctggaacggc ccgagacgac ccgaggcgaa
    14041 accggtgaag accgtcacca tgatgatcgc ggcgccggtg atgatccccg cggtcgttcg
    14101 gaggccgtac gcaacagcct ccgtgttgtc gtgcgtcttg tcgtactcct cccggatccg
    14161 gctgagcagg aacacgtggt agtccatcga gagcccgaac agcaccgaga acaggaacag
    14221 cggcagccac gcttcgatcg cctcggtctg ttggaagctg aacccgatcg cgttgaagat
    14281 gtcgatcccg acgcccttct ggaagacaag cgtgatcgcg ccgtaggctg cggccaccga
    14341 cagcaggttc atgatgatcg ccttgatcgg caccacgatc gagcggaaca ccaccatcaa
    14401 gaggatgaac gacagtccga gaacgaacgc gagcacgatc cactggtacg tgtcggtcag
    14461 atccaagaag tcggagaaga aggccgtgtt cccgccgacg aagacctcga cacccgggat
    14521 gtcagagaac acggaaggca cgacgtcgtc ccggagctcc gtgatcgctc cgaaggcagc
    14581 gtcgttctgc gtcgtcccct ggaagaacgc tgacacacgg atcgcggtcc catccggtga
    14641 cggagcgacc tgcgtttgcg ctgcgaactg cggcatctcg gccacccgtt cccggagatc
    14701 ggccaccgcg gcgtccacct ccggcgtgcc gacgtcaccc gtcacgatga tctcggccgg
    14761 ttcggtcagt ccgcccgcga attcgcgttc gagcgtgagg aacgcctcct tcgacggcac
    14821 gtcgtcgggg aacgtctcga tgctcgtgga actgcccttg ttgagctgga aggtgaagac
    14881 ccccagcgtg cccaggacgg ccaccgatgc gagcaggaag acgatcggcc gcttcatcac
    14941 tccccgtgtg aggcgatccc agaaaccgcc gcgcgggtcg tgatctccac cgatgtcgcg
    15001 gcgcacccgt tgcacgatcc gcgacgaact ccgacgaacc agacgtgtga tcaagaacgc
    15061 gctgccgacg atcgcggcaa cgacgccgac cagcacgacg tacggcggag ccccgacggc
    15121 tccgagcacc ccacccacga cgccgctgac cagcaggatc ccgaggaacg cgacgaccga
    15181 cgcgagacgg gacatccatg gccagtcggt gcggtccccg agcaagctca acaacgccgg
    15241 aagaagcgtg agcgccgcga gcaaggacat caccgtgacg aagatcgcgc cggacgcgag
    15301 gctccggaag atcgtcgtgg ggaggatcat catcccgagc agagcgagca cgacggtcgc
    15361 gccgctgaag aacaccgcgc gactcgccgt cgcccccgcg gcgccgatcg cgtcgagctt
    15421 cgggtagccc ttcttccgct cttcccggta cctcgacacg atgaacagcg agtagtcgat
    15481 cccgacggcg aggccgatca tcgagatcat gttcacgacg aacaggttga actcgaccac
    15541 ctgaccgatc acggcaacga gtcccaggga cagcgcgatc gcggcgatcg ccatcgcgat
    15601 cggaacgagg gcggccacga tcgcaccgaa gacgaccacc agcacgatga gggcggtcgc
    15661 gacaccgatg gactccccct tgcgggagtc ctcttcggcg atctcggaaa agtcggcgaa
    15721 gacggtcgcc tgcccggcca tcagtgtcgt gaaccccgct cgctcatgat ctgcgagcaa
    15781 ggccctgagg tcctcgacgt cctcggcctc atcgtccgcg atcgcgatgg ggatcagggt
    15841 cgacgtgcga tcctcggaga cgagacccgc ggccgcttcc tccgactggg cgacgacgtc
    15901 gtagtacgtc gccgggggct cggcgacgag actcttctcc gctgcgaggg caccccggag
    15961 ctcctcgacg aacgaccggt attccggatc gtcgatccgg agatcttcgg aggacacgat
    16021 cacgtactcg gtcccgacac cggcggtctc ggttgcgaac cgttcgtcga tgacctcctg
    16081 cgcccgaacc gactcggggc ggttggtgaa ctcgatgtcg ttgttcagga caccgcccag
    16141 cagagggacc atcacggcga ccgaggcggc gatcacgagc acccagatga cgagggtcct
    16201 ccaagggtga cggctgcttg ctcgtgcgag cgcttcgggg ttgatcctca tctcctggct
    16261 cccctctcgg cgggtcttgc ggaacgtaca ctgtacgctt acgatgtacg gagactaggt
    16321 gtacgtcgta agcctgtcaa ccccgaacga cggtgcgagc caagaacgag caagaatggg
    16381 acagcatatg aagttgaacg agacccgaag cagggaaccc ctcacgcgcg accgcgtgat
    16441 cgaagccgcg cttcgggtga tggacgagga aggcctcgag gccgtctcga tgcggcgggt
    16501 cgctcgcgag gtgggcgtcg aagcgatgtc gctctaccac catgtcgagg acaaggaaga
    16561 cctgctcgat gggatctgtg agcacgtcat ggcagggttc gaattccccg atcccgtcgg
    16621 ggactgggcc gagaactgcc ggagaggagc ccgcgcgtgg cgtcgtctcc tgcagtcgca
    16681 tcccgccgtg atgcgcctgt tcgccgacca aagaggcccg gtccgaacgg tggactcgat
    16741 gcgcccgatg gagttcgcgc tcaggctcct ccgcggcgcc ggcctttccg atcgtgacac
    16801 cgcgcaggcg ttccacgcgt tcggcggcta catccagggg ttcgtgatga tggagctcgg
    16861 ctcgatcgcg ggtggatccg acgaggcgca cctgaaggcc cacgcggacc tggtttcagc
    16921 cctcccggag gagttcgagg cgctcgtggc ggtatcgccg tacttcgccg aatgcgacgc
    16981 cgacgagcag ttcgagttcg gcctcgatct cttgatcagg ggcctcgagg ccaaggctgc
    17041 ccggaccgac tgacgtccag agggaaccag gctcggacgc tcggcgtcca agtccgaacc
    17101 gccgacacgg aggctcgcat gatggttcgg ctccccgcag ctctcgcgct cctgctcacg
    17161 ctcgccgcct gtgccgactc ccccgtcgat cccggtgtcg gcggcatcga ccactccacc
    17221 gcccccgacg aggtgctcgt ccggatctcg tacgaaggcg gcttcgtccc cgtcgagtgg
    17281 acctacacga acttcgcctt ctactcgctc ctcggcgacg gcaccctcat cgtcccgggc
    17341 gcacagatcg agatctatcc cggacccgca ctcccggccg tctccagccg gcacgtcagt
    17401 gaagcgggga tccaggcgat cctcgagcag gcgatggccg tgatcaggaa catccccgcc
    17461 gacctgaacg atctcggctt catgaacatc gccgacgcct cgacgacggt gatcaccgtc
    17521 tcggccgggg gcgtggatcg aacgatccga gcgtacgccc tgagcgagct gaccgagcgc
    17581 cccgacggga tgcccgcgcg cgagtaccag gcgcgcctca gcctccagga gctcgtcgcc
    17641 acgctgaccg gtccgaccga gtggctcccc gaggggtcgc tcggccccga gggcgcgtac
    17701 gaggcctccg cggcacgtgt gttcgtcggg aactaccggg ccgtcgacga cctcaagcag
    17761 gagccgatgc cgtggccgct cggcgccggg ctcgcggact tcggcgatgc gatcccgccg
    17821 ggcgggatgc gatgcggggt cgtcggaggc gagaactggg acaccctccg cgagtcggcg
    17881 gcggcagcga accagctcac cccgtggacc gacgccggcg accggtattc gatcctgttc
    17941 cgacccctgc tccccgacga gcggggctgc tgagcccctt ccctcggtga ggggctgagc
    18001 ggccggtcaa gtcccctcct caccgagccg atggttcggt gaggagcagg ccgccctccc
    18061 gcggccactg tgtggaccca tgacgactga gatcgagggc tccgccgtgg acccaccgag
    18121 gaccaccgac ggcttcttcg agccgcccgc tgtgggaagc gccgatcgca cccgggagct
    18181 cgagctccgc tatcgcggga tcatcgaccg gctgccggcc gtgctctacg tcgacgccgt
    18241 cgaccaggac cagccgatga tcgacgtgag cccgagcgtc cacgacctgc tggggatccc
    18301 ccgcgaggag ttcctgagca ggccgtacgc gtggaccgac acgatccatc ccgacgacct
    18361 cgccgcggtc atggccgaga acgagcgctc cgctgtctcc ggagacccgt tccgcatgga
    18421 gttccgcgtg gtccaccccg acgggaggat cctctgggtt cgcgacgacg cggtgctgat
    18481 ccacgacgga tcgggagcac cgctccactg gctcgggctg atgctcgacg tcacggagct
    18541 gatcgaggcc caagccgagc tgcatcaggc caagacgaaa tacggcgccc tggtcgagca
    18601 gatcccggcg atcgtctacg tcgacgcggc cgacgacgag atgtcgacca cgtatgtgag
    18661 cccccagatc cgggcgatcc tcgggtacac cgcacaggag tacatcgacg acccgcagct
    18721 gtgggagcgc atgttgcatc cggacgatcg cgagaacgcg gtcgccacgt atctgcgcgg
    18781 acgggagtcc ggcggcccct tcgtcttcga ataccgcctc atcgcgcacg atggcaggac
    18841 cgtgtggttc cgcgacagcg cgatcgtgct caccgacgag cagggccgcc ccgagcacat
    18901 ccagggcgtg atgctcgaca tcaccgagcg caagaacgca gaggagcgga tcgcgttcct
    18961 cgcgtatcac gacaagctca ccggactccc gaaccgcacg atgttcgacg agctcctcgg
    19021 gctggcgctc gcgcgagcga aacgccacga gctcggcgtc gtcgtcgtga cggtcgacct
    19081 cgacgacttc aagctcgtga acgactccct cgggcacgag gccggagacg cgatcctcct
    19141 gcagttcgcg caacggctca gcgacgcgac acgagagacg gacctcgtgg cgcgaccggg
    19201 tggggacgag ttcctgctcc tgctcgccga cctcgatcgt gagtcctccg gccacggcgg
    19261 ccatgagagt gtctccgtct ccgccgagtc ggtggcgctg cggatccaag aagccctccg
    19321 cacccctttc gccgtgggcg gtaccgagct ctacgtgacc gcgagcctgg ggatcagcgc
    19381 gttccccgac gacgcggtcg acgcgccgac gctgctcaag aacgcggaga ctgcgatgtt
    19441 ccggtcgaag cggtccgggc cgggcgggta cgtcctgcac gcgaaggacg acgccgactc
    19501 gatcgtgcgg ctgtcgctgt ccacgcggct gcgtcgcgcc gtggagcagg agcagtggat
    19561 gctgcactac caaccgctga tcgaccttcg cacctcggag atgaacggcg tcgaggcgct
    19621 gatccgatgg cccgaaccga acgggggcct cgtgccgccc ggcgacttca tcccgctcgc
    19681 ggaggagatg ggccttatcg aggcgatcgg cgcctgggtg atcgacgagg tctgccggca
    19741 gggtcgcctg tggcgggagc agggactgga cctcgacatc tcgttcaacc tgtcgccccg
    19801 gcagctctgg cagaacgaca tcgtggacct gatcgtcgat cccatcctcg gatcggggat
    19861 ggatccgagc cgggtgacgg tcgagatcac cgagtccacc gcgatgacca atcccgatcg
    19921 tacgctcggc atcctccacg agcttcacgc ccgcgggctc cagctcgcga tcgacgactt
    19981 cggaacgggc tattcctcgc tcgcccggct ccgctatatg ccggtcgacg tcctgaagat
    20041 cgatcgctcg ttcgtgcgcg agctcgatcg cgatgagcag aacgcgagca tggtctcggc
    20101 catgatcgcg ctggcctcca acctcgggat gacgccgctc gccgaaggga tcgagacgga
    20161 ggcggagtgg cgcgcgctca cgcagcgtgg ctgcgaactg ggccagggct acttcttctc
    20221 ccgacccgtg ccagccgcgg agatcctcgc gatccaccgg cgcgccgagc tccacgtcgt
    20281 ggagggcggc gccgccggat gatcgtggac gcgtcgtcgg cggtcagcgc cggcgacgcg
    20341 gccggagccc actccgcttc ccgcgcgccc cgccgctgaa cacgacacgc gggggcgaca
    20401 ccttcatccc ctcctgctcg aactccgcgt gcaggtcgag ccgtgccgcc atccgactca
    20461 cgtcaccgag ctgctccggc gtgacgaacg tgatgctcgt ccccgacctg ccggcgcgag
    20521 ccgtgcgtcc cacccggtgg acgtatccct tgtcgtcctt cggcgggtcg tagttcacca
    20581 ccaaggtgat ccgctcgagg tcgagacccc gggcggccac atccgtcgcg accaggatcc
    20641 cgacgtgtcc gtcctcgaac cggccgagcg cacgctcccg agcctgctgc gtcaggtcgc
    20701 cgtgcatcgc gacgaccgtg accccttcgg cgcggagctg tcgagccagg tggtcgacgg
    20761 cgcgcttcgt gttcacgaac acgagggtcg agcccgacgc ctccgaggcc agccgggcca
    20821 gggtcgacag cttgtcctgc ggcgccaccg ggatgaaccg atggtccgcc tcctcaacga
    20881 agcgcatctc gacggagccg acctcgtggc gcatggggtt ccgcgtgtac acctcggcga
    20941 tccggccgat cgcaccgtcg agggtcgcgg agaagaacat ggtctgccga tccttcggca
    21001 ggtggcgcac gatccgatcg acctgcggct ggaaccccat gtcgagcatc cggtcggcct
    21061 cgtcgaggac gaagatccgc acgccgtcga gcttgacgag gccgcgctcc acgaggtcgt
    21121 ccagccgtcc cggggtggcg atcaggatgt gcgcggaggt cacccctttc gactgttccc
    21181 tgaccttcgt acccccgtaa acggccatga cccggagctt ccgcgcccga gcgatgtcct
    21241 cgaactcatc gctgacctgc tgagcgagct cacgggtggg cgtcagcacg agggcggccg
    21301 ggcggcgctc cacgccggga tcgatctggt cgacgatcgg gatcgcgaac ccgagcgtct
    21361 tgccggaccc cgtcttcgac ctcgcgagcg tgtccctccc cgaggtggcg tcctcgatca
    21421 ccatcacttg gatcgggaac ggcgactcga tcccacgtgc ctcgagcgcg cgcacgacag
    21481 cctcggaaac gcccaggtcg cggaacgtga tcatcgtgcg gtgcacctcc tccttcgaga
    21541 gtaccgcccg cggcgacgcc tcgagccgtg ccggtagtct ctcggcgccg cgctcccgag
    21601 cacggatcgg aacgccgaaa gggaggagat ggacggcatg ggcgacagga acgggacgga
    21661 cgatcgcgtg gggctctgga ccgagctcgc cgcccagctg gctgtcgaca gcatccgatg
    21721 cacgaccgcg gccgggtccg gtcaccccac ctcgtcgatg tccgcggcgc atctggccgc
    21781 ggtcctgttc gccgatcacc tgcgaaccga cctcgccgac ccgcacggtg cggggaacga
    21841 ccgcttcgtg ctgtcgaagg gtcatgccgc gccgctgctc tacgccgtgc tgaaggcgat
    21901 cggcgcgatc gatgacgaca tgctgctgtc cctgcggcgg ctcggatctc cgatccaggg
    21961 acaccctgcc cccgtccccg agctgccgtg ggtggaggcc gccaccggat cgctcggcca
    22021 gggaccggcg atcgggctgg ggatggcgct cgggatgcgt ctgtggggaa gccccgcgcg
    22081 ggtctgggtc ctggtcgggg actccgaggc agccgaggga tcgatctggg aggcggtgga
    22141 ggcggccgcg caccaccacg tctccaacct cgcgtgcgtg ctcgacctga accggctcgg
    22201 ccagcgcggc ccgacgatgc acggttggga tgcggacgtc ttcgtcgccc gcgcgcaggc
    22261 gtacgggtgg cacgcgctct cgatcgacgg acacgacgtg acggcgatcg acgccgccta
    22321 ccgttcggcg gccggggccg atcgaccgac gatgatcgtg gcggggaccg agaaggggca
    22381 cggcgtctcg gaggtggcga accgggatgg ttggcacggg aaggcgttcc cggaggatct
    22441 ggccgctcgt gcgatcgacg agctgggagg cgtgcgctcg ctcacgatca cgccgcagaa
    22501 gcccgaacca tggatgcccg caggtcgggt cgcgccgaca ggcgcgcccg cacccaccta
    22561 cgccgagccg atcgccaccc gcaaggcctt cggagagacc ttgcggtggc tcgccggcga
    22621 gcgcgacgac ctcgtcgtcc tggacggcga ggtcgggaac tcgacctaca cggaggatgc
    22681 agaggccgtg gccccggatc gcttcttcca gatgtacatc gccgagcaga ccttggtcgg
    22741 ggcgcagacg ggcctgcacg cgctcggcgc caccgcattc tccgcgacgt tcggggcgtt
    22801 cctcacccgc gcctacgacc agatccggat ggcggcgatc gggcgggcgg atctccgcct
    22861 gtgcggttcg cacgcagggg tgtcgatcgg cgaggacggg ccctcgcaga tggcgctcga
    22921 ggacctcgcc gccctccgcg cgatccatgg gacgaccgtc ctctacccat cggacggcaa
    22981 cgccacggtc gggctcgtga cgtcgatggc cgacctccgc ggcatctcct acctccgcac
    23041 gacgagagag gccacaccgg tcctgtacgg gccggaggag gacttcccga tcggcggttc
    23101 gaaaaccctt cgcgagggcg acgacgtgac gctcgtcggt gcgggcatca cggtccacga
    23161 gtcgctggaa gccgctgacc gtctcgcggc cgacggggtc gaggcgcgtg tgctcgattg
    23221 ctacagcgtc aaaccgatcg acgcggagtc gctccggaac gccgcagccg cgaccggact
    23281 gctggtcgtg gtcgaggacc acaggatcga gggcggcttg ggcgacgcgg tgctcgacgc
    23341 gctcgccgcg ggagggacgc tcgccgggcg ggtggtcaag ctcgccgtga cggacatgcc
    23401 cgggtcgggc gcacccgagg agctccgggg ctgggcgggg atcgacgccg acgcgatcgt
    23461 gggggcggtg cggagcgcgc tcggccgaga ctgaggtctc gtcagctggc ttcgctcgcc
    23521 acgtggagac caccgaagga catggcgatg atctcctccg gcggcacggc cttcgagaag
    23581 tggaaccctt gtcccatcac gcacccgcgc tcccgaacga acgcgagctc tccctccgtc
    23641 tcgatccctt cggccagcgt ggtgacgccg agccctcgcg ccagttcgat gaaggcggtg
    23701 acgatgctct gcttctgcgg atcgacgtcg acaccgctga cgaacgaacg atcgatcttg
    23761 aggacgtcga ccggcatgtc ccggatccga gcgagcgagg agtacccggt tccgaagtcg
    23821 tcgatcgcga tccggagacc accatcgtgg agcgcctcga ggatcatctg cgcgcggtcc
    23881 gggtcggtca tcgccgagga ctccgtgatc tccacgataa ccttcgcggg atcgatgccg
    23941 ccttcgcgga tccgatcgag gatcccgctg gcgaggtctg gctgccagaa ctggcggggc
    24001 gacaggttga agctgatcga gagatccatc tccagctcac gccaggcctg cgcctgatag
    24061 acgagctcgc gcacgaccca atcgccgatc gcttcgatca atccgagctc ctccgcgacc
    24121 gggatgaagt cgcccggcag cagcatcgtg ccatcggcct cgatccaacg gatcagcgcc
    24181 tccaccccgc gcatgcgtcc ggtcgtgagg tcgaggacgg gttggtagtg gagggtccat
    24241 cgctggctct ccacagcctt gcgcagcctc gtcacgaaac ggagcttggc ggaggagtcc
    24301 agggcgccgc ggttggacac gacgtacgcg tgcccgccct tcttggactc cagcatcgcc
    24361 gcgtccgcgt tccgaagaag gctgcctggc tcatcgccgt cctgggggaa caggctgatc
    24421 ccgatcgagg cggagacgta gagctcggtg ccgcccgctc ggaagggcac ggtgagggag
    24481 tcgaggatcc gctgcgccga cgactccgca cgggcgacgg ccgcttcgac gtcgtctccg
    24541 tcgcgctcga ggtcggcgag caggagcagg aacgtgtccc cggaccggcg agcgaccagg
    24601 tccgtctccc tggtcgcctc gcggagccgt tcggccacga tgttgaggac ctcgtccccg
    24661 gcgagccgac cgagggagtc gttcacgagg cggaaatcgt cgatgtccac gcagatcacc
    24721 gccacggagt cctcgtggtg cttcgcacgt gcgatcgaga gctcgagcag ctcctcgaac
    24781 atcgcccgtg tcgggagtcc ggtcagctcg tcgtgatagg tgaggaacgc gaccttctcg
    24841 tgcccgcgct tcatctcggt gacatcgaac atcaccccga gtgagaacgc ggggatgccg
    24901 cgctcgtccc ggacgatgcg cgcctcgtcg cggatccaga tgatccgccc gtccttgcga
    24961 tacatccggt aatcgagcgc gaacgattcg ccggtctcgt tgtgacgctg gttctcggcg
    25021 atcgctcgcg ggcgatcgtc cgggtggacg atccgcagcc acagatccgg gtcgccgatc
    25081 cactcctccg gcgcgtagcc gagcagctgt tccacctgcg gactcatgta gatggttgac
    25141 gccgcgtcgt cgagcgcgtc gatgtaggtg atcgcgggga tgtgctcgac gatcgagcgg
    25201 taccggtcgc ccacgatctc gtccgcggac gcctgacgct gccgctggat gtcggccccg
    25261 atcgatcgag ccaacacgcg caggagctcg atctctccgg tcccccacga ccgaccgccg
    25321 ctgcagtcgt ccacggtgag gtacccccac cagccttcgt cgacgacgat gggaaccgct
    25381 acgaacgaca ccgttccctc cgccgcgagg atccgccgct ccgactcggg gaggtcgctc
    25441 acggaggcgc tcagcacccg atgatccccc aggacctcga tccaccggat gaagtcgggg
    25501 gagtacgggt gcagcgtcgt gctcgggtcg tcgaagatcc cccggacacc gttccgcctc
    25561 cattccgagg tgagatccat ccagaggcgg ccgtcggggt cgcggatgtt ctcgaacagg
    25621 gcgacccgat cggcgccgag ctcccgcccc acgttcgcga gcgcatccga tacttgggca
    25681 agcggggacg cggcgctcga cacgccggga tcgaccgccg gcacggcggc cgaggacgag
    25741 ccctgcgaga cgtccatccc ccctgctatc ggcggacccc gagggccgct gaaggagcgc
    25801 ccaggcaccc gtgcctgcta ctcttccggg ccgatggccg actacaagca ggttctccac
    25861 gagcgtctct acgtgcgcgt tccgggcacg gacaagctcc ggaagactgc tgcggcccgc
    25921 ctgaagcacc tcgtcgccac gggatggcgc gagaccgagc gcgaccgcaa gcccgaccac
    25981 atctgggtcc gcttcgagcg tacgggccac gccccgatgc gcgctcggct gcccaagaag
    26041 gcacccgaga tccagatcga acgccgacct cgtcgggaca accgtggtgg cggcttcagg
    26101 ggcggccggc gctagccgat acccctgggt accggacatc ttccggtgaa ccggcccttt
    26161 gaccggactt ccttcgggta ggatgacgtg gtccgcacat cctgtgcggc gactcgaccg
    26221 acgacgatcc tgcgaggacc tgatgagcgt ggaattcctc agcccccctt cgggccggcc
    26281 cggacaccgc gatccccatg cccacgccgc cgcccagccc ttcagctatc cgctgcaacg
    26341 tccgtacgcg gaaccggact ggacgcgcct cgatgcgtac cgcgacgtca ccacggagca
    26401 gtgggagtcc gcccagtggc agcgggcgca caccgtcaag aacctgaagg agttcaaggc
    26461 ggccctcggc gggcacctcg acgacgaact gctcgcggac atcgaacgcg accaacagga
    26521 gcgggcgacg atgtccatgc tcctcccgcc ccagatgatc aacacgatgg acgagaccga
    26581 tctccgggcg gaccccgtcc ggctgtacat ggcccccgcg ttcagcgacc gggatcagga
    26641 gttcccctcg caccctttcg cctcgcgtga ttcgctccac gaggccgaca tgtgggccgt
    26701 cgagggtctg acccatcgtt atccgaccaa ggtcctcgcc gagctgatcc cgacctgtcc
    26761 gcagtactgc ggccactgca cgcgcatgga cctggtgggc aacgacacgg tgcaggtcct
    26821 caagtacaag ttcgaggcga agcagaacga ccgctgggaa gcgatgctcg actacctccg
    26881 gcgaacgccg agcgttcgcg acgtcgtcgt gtccgggggc gacatggcca acgtcccgat
    26941 caagcggcta gaggcgttcg tgatgggcct gctcgacatc gagaacgtcc gcgacatcag
    27001 gctcgcgacg aagggcctca tggggatccc ccagcacttc ctgcaggacg aggtcagggc
    27061 ggcggcggaa cggatggcct cacgggcgag cggacgcggc gtggacatcg ccgtacacac
    27121 ccacgtgaat gcggcacagc aggtcacgcc gctggtgggc cgcgcgacca aggtgttcct
    27181 cgacgctggg ttccgcgacg tgcggaacca gggcgtgctg ctccgcggtg tcaacaccgc
    27241 tccacagcag ctcctcgagc tctgtttcat gctcctggac cacgcgaaga tcctccccta
    27301 ctacttctac atctgcgaca tgatcccgaa cgccgagcat tggcggacgg cggtgtggga
    27361 ggtccaggac ctccagcacg cgatcatggg ctacctgccg ggcttcgcga caccccggat
    27421 ggtcgtggac gtgccgttcg tcggcaagcg ctgggcccat caggtcgccg agtacgaccg
    27481 cgtccgcggg atctccgggt ggacgaagaa ctaccggacc gggatcgaga gcgacgaccc
    27541 cgaggcgttc acccggcgat accactacta cgaccccatc tcgaccctgc ccgaggacgg
    27601 caaggcgtgg tggcgggaac agctcgcgcg aggcgtggac gagctcatcg aggagctcca
    27661 gccgcagcac acctgatcgg gggtctgggg ctcaggagcg agcgcgagcc gcccctcggg
    27721 gatggatgcc cgctgctaag gtccggagcg tgagccgcgt cctcaccgta ttgtcggccc
    27781 aggtcgcacc cgtcgcctgg gacccggcgg caacgctgga caagttcgag gatcacgtgc
    27841 gcgtggcccg ccgcgcgttc ccggacgtcg acctgatgat cttcccggag ctctacctga
    27901 ccgcgatcga cgggttcacg tcgggaggat ccgccgattg ggagcgccgc gtcgccgagg
    27961 agatccccgg gcccttgacc gaccgtgtgg cgaagatcgc cgctcgagcg aagcggtgga
    28021 tcgtggccgg gtcgatcaac gagcggcacg ggaagaagat ccacaacacc gcgatcgcct
    28081 tctcgcccga cggggagatc gcagcggtct accgcaagct cttcccgtgg cgcccgttcg
    28141 agaacgccga cgccggcgcc gagccggcgc cggtgttcgc gatacctggg aagggcgtcg
    28201 tcggcatgat gatctgttac gacggctggt tcccggaggt ggcgcgaggg ctcgccctcc
    28261 agggcgcgga gttgatcgcc caaccgaccg cgaccacgac gcccgatcgc gaggaagaag
    28321 tggtcctcgc gcgagcgaac gcgatcgtga accaggtctt catgttcaac cccaacatgg
    28381 tctcgacgtt cgggccggga cggagcgtcg gcgtcgaccc cgaggggcga gtcctgttcg
    28441 aggccggcgc cggtgaggag ctgctggtgg aggtcgtgga catggatcgc gtggccgaga
    28501 tccgccgtcg cggcacgcgc ggcgtgtcac gaccgtggga gcatttcctc gaggggcccc
    28561 gggacgtggt gctccgcccc taccgggcgc gacgcaccac gtagccggcg atacccagcg
    28621 tcgcgacggc cgcaccggtg acgagccacc acatcgacag accggatcgt gtatcccctg
    28681 ctggttcgtc atcgcccacc ggctcgatcc cgacgtcgaa ccgttccgtc ggcttcccgt
    28741 ctccgaggac atcacaggca cggccgtcgc gcacgatcac ggtccaccgg agcgcctcga
    28801 gaccgggcga ggtccccgtg agatcctcgt cggcgctcaa cgtccgaccg gggatctgga
    28861 tcgtcatcgt ggcgccctcg atgatcggag gattgaacac gttcgcgccg cgttccggga
    28921 ggaggtagat ctcggtctca tgggcgacgg tcgcgtcgac gcgcaccaac cggttcacgt
    28981 ccgtgtagta cgcgaacgag agcggtggaa cgtccgggtc cctgatcgcg atgtccaccc
    29041 ggaacggtcg tccgaccgca tccggtaccg tcagcggctc cgcgaaggtc agcgtccagc
    29101 gagcggacgt gccgagctcg acgatctccc cgcgtgcgtc cacgaggtcg ggcggtgtcc
    29161 cggggccggg cggggcaccg cacccggcca cgtcgccggt gggatcggat gcggccacga
    29221 ggagcgggac gatcgcgatc acggccgcgc ggcgcgccgg gccggcgcgc gacatcagga
    29281 cgggcagtcc cgcggttcga tgatcagcgg gtcgagacgg ggttcctgct cgaagtcgaa
    29341 cgctcccgag agcggatccg cctgccggtc cctgtcggtc atcggcttca gaccgtgcag
    29401 atcctcgatg aaccggagca cggaggagaa ctcgtacacg gtggagtcga tcgacccgcc
    29461 gtccgggttt tcgcccgcct tcgtccacgg cgagatgatc aacgcgggcg tccgtgggcc
    29521 gagccccatg atgtcgtagt gcggcggcac gacgtggtcg tagaagcccc cgaagtcgtc
    29581 ccagacgatc acgatcgcgg tgttcggcca ggcgtcgctg cgcatcaccg cattgaggta
    29641 ctcgaccgtc cagttctcgc cttcgcacac gttggtcatc gatcccgggt gctcgttcgg
    29701 atttccctcg ggtgggatca gccaggagac cgccgggagc tccccgcggc gaacgtcctg
    29761 gaggaacgtc tcggggggca cgaccttgtc ccacatgggg ccggatcgca cgtgctccac
    29821 cgcctgcaac gcgttcatcc acgaatccgc attcgcgtag tacctccagc tgatcccctt
    29881 cctctcaagc ttgtcgggga gcaccgggat gttgatgcat gtccgtgtcg attcccaata
    29941 cgcggcgatc cggatcaatt gccctggaac gtccgacgtg atgtcttcct cgagggccat
    30001 gatcctctcc acgtccgcgt ccgtgaggtc ctcgatcggg aaccgcttcg tgatctccgt
    30061 gtcgtcgtcg cagtagttgc cctctacgtt ggtggtcgtc ttgttgtcga cgatgccgta
    30121 ggactgcgcg gcaaccgtgt agaggtgctc ggggaacgtc ggcccgtaca tcgacgtgaa
    30181 gaactgatcc gcgagcacga accggtccgc gtacgcccaa taggcgggca gggtctcccg
    30241 cgagtgctgc aagtagccca tcaggtcgtc gcccagcgac acgcaattga agccgttcat
    30301 cttgccgccg ttgatcgagt acaacccggg agcgaacgcg tggccgaggt cgtgcgggag
    30361 gacgagcggc gcttcctcga ggtcgatcac ggggccatcc ccgaatgccg tcgcgtcaca
    30421 gtcctcgatc gtgcctcctt ccgtcgctcc ctcggcaccc ggatacgtcc cgaagtagtg
    30481 gtcgaaggac cggttctcct tgacgaggaa gatcacgtgc tggatcggcg agttcggatc
    30541 gggtgagggg gactggatgc cgttcccgtc atcaccgggg tcgggggtct cgcctcgttc
    30601 ctgaccggcg gagggccaga actgccatgc gaggatggac gtcaggacga cgacgagtgc
    30661 cccgaacgtc tgtcggcgtt ggcgaacgcg cgcgcggcgc acaggtcgga tgatcggccc
    30721 gggtcgcatc ggctcagtct agccggggcc caggacgcga ccgaggaggg cgcgcaggcg
    30781 ccccaggagg cccgcggcgc gcggatccgt cgcccggttg gtcgtctccc aagggtcggc
    30841 gggccaccgc ctcccgctca ggtcgtagag ctcctcgaag ccgcccgcgt accggatcag
    30901 cttgaagtcg gaggtgcgga cggccgtcca cgccgggatc cgctcgtcgc ccgcccactc
    30961 caggaacacc gcgtcccccg ggtcgcccgt gacgtctccc gccccgatcc tccccgcgaa
    31021 gctcgttccg tccatcgtcg cgggcgcccg gacgccggcc agatccagga tcgtcggagc
    31081 caggtccacc atcgagacga gctcctcccg ctctcccggg cgcagcccgg aggcatgcac
    31141 ggcgagggga acccggacgc acgcttcgta cggacacgtc ttgccctccc atcggtgctc
    31201 cccgaaggaa tagccgttgt ccgacagcac gaagatcacg gtctcgtcca ggcggtcgcc
    31261 gagcgcggac acgagcgcgt ggagcgcctc gtcgaccgcg agcagggtct cgtccgcacg
    31321 ccgctgttcc tcgagccagg cggtgcgctg cgcatcgctc ggcgccggca acgagcgcac
    31381 ccacggcggg gcgccgcgca gcgtccccgc cacggacggt ggctcctcga tcgccaggcc
    31441 ggcgaggatg ccgtcgtgcc gggctgccgg gatccacgga tggtgcgggg cgctcggcgc
    31501 gaacaggagg aagaacggct gcgacggggg cgcgctccgt acgaatccga ccgctcggtc
    31561 cgcgagccag tcggtcgcgt agcgacggac gaagacaggc gacccctggt cgacggcgtg
    31621 gaagtctcga tacaccgtcg ccgcgatctg gttctgcttc gccaagaacc ggtcccagcc
    31681 cgcagggacg aacgggagcc gcccgaacgg gtagcggttc aggtacttcc ccacgaggcc
    31741 tgtgcggtag ccctcgtcgt ggagccacgt ggcgagcgtg gacgacgcgt cgaacctcgc
    31801 accgtccccg ttgtcgagga caccggtgtg ccgagcgaac aggcccgtca gcaagctcgc
    31861 ccgcgccggg cagcacagcg gtgtgttggc gaaggcgttc gggaaccccg cccagtccgt
    31921 gccggggcgg gcgagctgcg acgcgagcca cggcatcgcc gcgaaggagc ccgctcgctg
    31981 gtcgtcggtc aggacgacga tcacgttggg tcggtcgtcg gagcgcccgt ccggctgcgg
    32041 agcgaggagc gctgcgccga gcccgcccaa cgcgacgagg gccgccccgg ccatccgacg
    32101 gccgggcggc gcgagcctca ctccttgcgc accttccaga tgtgttcccc ggggaacgct
    32161 cgggcccact cgaggggtgc gtagtcgtcg taggtcgtgg tcgcgtccga gtccggccga
    32221 agctcgatca ggtcctcgat cacgagcccg ttcgcgcgga acagccggat ccagtccccg
    32281 taggtcagct ggaactcggt cgtccgccac gacgggtcat cggactcgat cgaccgcatc
    32341 tcgaagtagt ccgcgcgcat ctcccgggtc gtggggagct cctcgtcctg gtcgccccac
    32401 gcgacccaga tccacggcgt cgtgccgttg aagacgaaga ggcccccggg ccgcagcacg
    32461 cgagcgacct cgggcaccgt caggaaggga tcggcgaacc ccatcacgcc gtggtcgcag
    32521 aacaccaggt cgaacgagcc atcgcggaac ggcacgcact caccgctcgc ctggacgatg
    32581 gggaagcgca ccccggtctc ctcgaagtgc gccatcccgt ggcggagttg agcgaacgag
    32641 agatcgagtc cgctcacgtt cgctcctcgc atcgcgacct tgatcccaga ttggcacccc
    32701 ccgcagccga actcgaggac gcggcggcct cggacatcgc cgagcgcgtg gatctcgtcc
    32761 tcggggatgt cgtacgtccc ccagcccaat cgatcccagc ggttcagctg cgcgcgattg
    32821 cgctcctggt actcgtccgc ctcgcgctcc cagtgctcgc ggttcttgcg cacgtgttcg
    32881 ttcgtgtctt gcgtcaccgg tccagccacc ctccctcgcg gtcgtgatcg gcggcgacga
    32941 gccctgcggc gatggcgagg gcgatctcct cgggccggcg cccgccgagg tcgagcccga
    33001 gcgggctccg gatgcgacgg agatcgacct ctccgaaccc gcgactccgc agatcctcga
    33061 cgtagtgggc gacgtgacgg cgggaaccca tcacgccgat gaaccggacc tgggagcgca
    33121 ggagcgcggt cagcatgtcc gcgatcccgg gtgcgtcgtg atcggtgaac acgacctcgt
    33181 cgtcgtaccc gagcgtcagc tcctcgagcg atccaacgga cggcccgtcg tcaggagcga
    33241 cccgttcggc tctgggctcg agcacgatca cgcgtcgtcc ctggcgctcc atctcgaccc
    33301 ggagggacct ggccacgtcg gtggcagaca cgacgacgag ccggggaggg gcgcggtgcg
    33361 gctcgagata cacctccaca tccccttcgg ggtgatggag cgtgcgcatc tcgggtgctc
    33421 ccgcggcata gacctcggcg gccgcggtga cggcggcggc gtcgaactcc gcacagccga
    33481 gcgtgccgcg tacctccccg ccgggcacga tcgccatggc gttcccgacc gcgcagggag
    33541 gcgagccgat cacccgcacc gctttgacgt cgacccacgg acccggcgcc cgttcctcga
    33601 tcgacgtggc cgtcgcggcg ggggctgtcg cgtgctggtc ggggatgctc ggatggggtc
    33661 ccgtggcgcc gccgaggagc tccacggcgt gcggaaccac cgggagcacc gccgcgagcg
    33721 actcacggac ccctttggga ctgcccggga ggttcacgat cagcgcggcg cccacggcgc
    33781 cggcgatccc gcgggagagc gcggccatag gcgtctgcgc cagtcccgtg gcccgcatca
    33841 gctccgcgag cccgggagcc tcgcgctcga tgaccgcccg cgtcgcttcc ggggtcacgt
    33901 cgcgcgggcc gaaccccgtc ccgccggtcg tcacgacgag cccgttcgcc gacgcgagct
    33961 cccggagcgc cggctcgatc tcctctcgct cgtcggcgac cacgcggcgt tccgacactt
    34021 cgaagcccgc ggcgcggagc aacgcctcgg ccgcgtcacc ggaggcatcc tctcgcgtgc
    34081 cctgggtgac gccgtccgag acggtcagaa cggcggcgtg ggtcgtcccc gattccacgg
    34141 tgggagccta gcgccgcggc ggagccgctc ggttccatgg gtcgggggac gaccgtccta
    34201 ggattggggg aactcgcggg atcgccccgc ccgcgcgagc gaccgaggag gcaccgagat
    34261 ggaatgggtc gaccggctcg ccgccgagct caagctcgac gtgctttccc cgcacgagaa
    34321 ggaccatctc ctgacggcat cccgcgaggt ggcgcaccgg gtcgagcgga aggccacccc
    34381 cctcgcgatg tacgtgatcg gactctccgt cggcagccag ctctccgatc gcgcacggga
    34441 cgactcgatc gaggacgcga tccacgccct gctgctgcgc ctgccccccg ccgacgagag
    34501 ctgaacgggt tcgcgctccc ggcggtaggc tggctcccat gaccgaccgg atcccccacg
    34561 aggcgccgat gccgctggac gggccgctcg tggacaccta cgggcgggtc gcggacgacc
    34621 tgcggatctc cgtcacggac cggtgcaact tccgatgcac ctactgcatg cccgccgaag
    34681 gccttgcatg gctcccgaag cacgagatcc tgaccttcga ggagctcacc cgggtgctcg
    34741 gcatcttcgt ccggctcgga gtccgcagcc tcaaggtcac gggcggcgaa ccgctcgtgc
    34801 gcgccgacct ccccgtgctg atccggatgt tccgcgaggc cggccccgac ctcgatatct
    34861 ccctcacaac gaacggggtg ctgctggacc ggctggcggg accgctggcg gaggcgggtg
    34921 tcgaccgagc gacgatctcc tgcgactcgc tcctgcggca tcgtttcgag gagatgaccc
    34981 ggcgcgacgc gctcgacaag gtcctcgccg ggatgcgggc cgcggaggcg gccggcctca
    35041 caccgatcaa gatcaactgc gtggtgatcg gcggcacgaa cgacgacgag gtcgtggact
    35101 tcgccagctg gtcgagggag accggcttcg aggtgcgatt catcgagtac atgccgctcg
    35161 acgcgcagca cgcgtgggag cgcgagaagg tcgttccggc ggctcgcatc ctcgccgcga
    35221 tcgacacggt gcaccccctc ctccccgagg gacacgcgaa cgagccggcc acgacgttcc
    35281 gcttcgccga cggggcccct ggacgggtcg gcgtgatcgc ctccgtcacc gagccgttct
    35341 gcgacacctg caaccgactc cggctgacgg ccgaaggcat gttccggaac tgcctgttcg
    35401 cgatgggcga gacggatctg cgagcgccga tgcgtggagg tgcttccgac gacgacctcg
    35461 cgctcctgat caggcagggt ctgtgggcga agtggtccgg ccaccggatc aaccacccgg
    35521 acttcgtgca acctgcccga tcgatgtcga tgatcggcgg ctgatcgcgg caggctccgt
    35581 caccgctcat cgtctcgaca cggggcgttc acctccggct gagaacctgg cgccgccgtg
    35641 aaggctgcgc cccctctccg gcgcggggag ccggccgcag gccggctccc cgcctacccc
    35701 ctcagaggtt ctcgatcggc agcaacggca cgtcgtcgcg gtcgaagccg aggacctgcg
    35761 cgtagaacga gagctccgcc tccagcgcga tcctcgtcga ctccgccttc cggaacccgt
    35821 gctgctcccc ctcgaacagg acgtaggcgt acggcaaccc tttcctccgg agcgcctcga
    35881 ccatgatctc ggattgatgc ggaggaacca cgcgatcctg gtcaccctgc agcaggagca
    35941 tcgggcacga gatcccgtcc gtggcgtgga tcggtgagcg ggcccggtag cgctccgcct
    36001 ccgccggata cggaccgacg agggagagca ggtaggccga ctcgaacttg tgcgtgcctc
    36061 cgctcacgaa cgactcgagg tcggcgaggc cgaagtagct ggtcccggcc gcgaacccct
    36121 cgtgcatcgt cagggcacag agcgtcgtgt agccacccgc gctccctccc ctgatcagga
    36181 ggcggtcacc gtcgacctcc cctcgggagg ccagccacgc cgccgcgttg atgcagtccg
    36241 cagtgtcgac gatcccccac atgccctgga gccgctgtcg gtatgcccgt ccgaaccccg
    36301 tgctcccgcc gtagttcacg tccacgacgg cgaatccgcg gctcgtccag aactgggtct
    36361 ccagggagca gcgcggcgtg gcttcggccg tgggtcctcc atggctcatc acgatcacgg
    36421 gcggccgttc cccctcaggt ccgaccgcgc cgggagcgtg cggcggatag aaatgcgcga
    36481 acgccgtcag tcctccctcc gtggggaact cgatctgtcg tgggatcgag atcgcggctt
    36541 cgtcgacgac cgaggacgac gagcggagca cgtcgatcga tcgagcggtg acgtcgagca
    36601 cgacgatctc gtcgggggtt gccggcccgc cgccgacgaa caccacccgg tctccctcaa
    36661 catcgagccg aggcgccatc gcggtgtagg ggacgtcgag gtcgatgagt tcaccggaca
    36721 gcggatcgag gatcgccagg tgctgaagtc cgtcccgttc ccacagacag gcgatccgcc
    36781 cgtcgctcag gaacccgtac gcgctcatcc cgaagaccca ctgaggccac ccgaactcgg
    36841 catccgccgg atggagccct tggacgtcac cgtctcgctc gacggagagg ttccaccagc
    36901 cggttcgatc ggaggcgaag tgcaggtcgc cgttggggct ccagctgggc tggaagatcg
    36961 attcctcgcc cacgaggccg gcgacccgcc ggacgcccga gagactcccg tccctcgcga
    37021 gctcgccgac gacgagctcg cacccgtccc acggcatcca cggcaggtcc cactcgagcc
    37081 agctgagccg cgagccgtcg ggcgcgatcc tcggcgtcga gtagaagtcg cggccggagc
    37141 ggatcgtgcg cggctccgag gagccgtccg gtgagagcgt gacgagctcg ttcacgaccc
    37201 cggcttgatc gtcgggctcg ggatgacgtt cgcggacgca gaccagcgat cgcccgtcgg
    37261 gggtgacccg tccgtccgcg taccgatcgc ggcctcctgt gtcgggcgtg atcgggaccg
    37321 ggtcgccgcc cgcatcctgc cggtacatgc gctgatccgc gaagttggag aagtagacgg
    37381 tgtcatcgtg gagcaggtag gccccaccgc cgtactcgtg cacggtcgtg cggacgttga
    37441 accccggggg cgtgacatcc gtcggctcgg agaagacgtc ggcgcgaacc aacacggacc
    37501 gtcctccttc agccgggcga ccctccaacc agtacgccac gccttcgtgc aggtgcggtt
    37561 gcatcaggga gacggcgccg gccgacaccc tctccgcggt gatcggcgag cgccacgtgc
    37621 cgaacggtgc cgcgaccgtc accggggacg tctcgcgacg atgacgcgcg tcagcggcag
    37681 cgggccaccc ttcgtgctgc ggatgtcgcg gaacccctca ccgcgcagca cgcccttcac
    37741 gtggcgcact cgatcggggc tgacctcgag caggagccag ccgttggccg agagccacgt
    37801 cggcgcctcc cgcgcggacc gggagatcag cccgagcccg tcggggctcc gatcggtcag
    37861 cgtgtgctcc ggctcccatt cccggatctc ctcgggcagg tccacgagct cgtgcatcgg
    37921 cacgtatggc ggatgcagcg tgacgacgtc caccgttccc tcgagcgtcc gcgggaggcc
    37981 gccgaacagg tcgccggtga agaagcgggc tcgcaccccg agccgcctcg cgttcgtgcg
    38041 ggcgagcttg accgcatccg cggagaggtc cgtcccgatc acatctgcgc cgcggacctc
    38101 gttcgccacc gcgagcgcga tcgtccccgc cccggtcgcc aggtcgagat ggaccggccg
    38161 cggccggccc cgcaggcgcc gcatcgcctg ctcggcgagg aactccgagg cgtcccgggg
    38221 cacgaagacg cccggacgca cgatcagatc cagtccacgg aactccgtgg agcctttgat
    38281 caacgggatg ggctcaccgg tggcgcgacg agcgaccagc cgttcgagcg tccgtcgctg
    38341 ctcgacggtg acgtcgtcgc gcggatccgg ctcctcgccc aacacatgcc agagcaggtc
    38401 ctccgcctcg atccgctccc gtccacgctg ccagtggtcg atcgccggcg aggccttcag
    38461 gaccttgacc gcgtcgtcca ggaccgtgcg tgccttcaac gccgggcatc ctaccgagtg
    38521 acgctcgcta gactcggatg tatgcccgga acgacgccgg ccgatggcgc gaagtcgacg
    38581 aggacggcga agcctcgtcg cgcacgcgcg aggaagtcgc cgtcgacggg ggaggatctg
    38641 cgtctgaccg gagaccggct cgtcgtgcag ttgcccgaga acggcgagcg gaagtcggcg
    38701 agcggcctgc tgatccccgc caccgccgcg ccggcgccga agcgtctggc gtgggccgac
    38761 gtcaagctcg tgggcccgga cgtgcgcgtc gccgcccaag gcgaccgcgt cctgttcctc
    38821 ccgtcctcgg gcctcgaggt ggagctggac ggacaggagc tcgtcctcct gcgggaacgg
    38881 gacgtccagg cggtctcgtc gccgccggcg tcggacgcac gctcccagcg agctccgggg
    38941 cagtatctgt agcggcgccc ctgttagcgg cggctcctct tcagccgtct atgaacgtga
    39001 gccgcacgcg ccggatgtcg ttctcgcggt tcggatcgag caggacgatc gactgccacg
    39061 tcccgagcac gagccgcccg tcctccacca ggagggtgag cgtcggggtc acgaacaccg
    39121 gcaggagatg atcgcccccg tgacccagag agccgtgccg gtgcgtgtat cggtcgtcgc
    39181 gcggaagcag ccgttccagc gccgcgtcca ggtcctcctc cgagccgctg cccgtctcca
    39241 tcagggcgag ccccgccgtg gcgtgcggga ggaacacatg gagcaggccg tctccgcccg
    39301 acgctccggc gtatgcccgg acccggtccg tgaggtcgac cacgcggcgg cccttcgtcg
    39361 aaagctcgaa ctcggtcgtc ctcacgcgcc gaacctatcc gccgccacgg tggggtgccc
    39421 ggaccgtgtg tgctcggcgc cgtgtaatga tgtaatgtcg cttgcataca aacccgagga
    39481 ggcaccgatg gaggaggagc tgagcggatg gttcgcaggt cggatcccgg acgactggtt
    39541 ccagggggca ccgaccgtca ccgccgaccg cgaggagatc tacgtggtgg ggacgctggc
    39601 ggagcccgag ctccccgcgg acgcgggcga ggaggcgcgt gaggcggcgc tcactgcccg
    39661 tatctcgggg ttccgagagg acacccgtcg cgcgcggatg cgcatcgccg acgaggccca
    39721 gaggcgcttc ggccgcaagg tgagctgggg agcgagctgc ggagacctca cagcggcctt
    39781 cacgaccctg tcggtgcccg cgatgacccg cctgcggatg gaggagcgtc gtgtgctcga
    39841 tacgttgatc gaggccgggg tcgcccggag ccgaagccac gccctggcct ggtgcgtccg
    39901 atcggtggcc gagcgccagg acgactggct gaaggacctc cgcggcgcgc tcgagaaggt
    39961 ccgagaggtg cgcagcggcg ggccgacgac cggatagggc gtccagaggg cggcgctcaa
    40021 gttccccgca cggggagccg accttcgagg tatggccgag aggggacgtg gggttcgccg
    40081 tcgatcgaca tccatcgcgc tgctgacgct cctcctgtcg gtggtgcaac tgctcggagc
    40141 gacgaccgcg aacgcgacac cgccctcctg gaccctgggc gaggacggga gtggtccccc
    40201 accgttgtcg acccatctct ctcgatcgga gcgtgctctg atcccgacct ccacctggag
    40261 tgacctggaa ccggacgatg cgtgggccaa ggtcgcgatc aaccacgtcg ccgggaccca
    40321 caactggatg cgggacttcg cgctggacca gaacgggaac cggcgcttcc gacccgacgc
    40381 gttcgagtcg agggcacgct gggcacgggc catggcccgc gcgttcgcgc cgggggagga
    40441 acccgatccg tcgatcacgt tcacggacct cgatccgtcc gatccgttcc atcgatccgc
    40501 cgcgatcgcg gtgcagcacg gctggatgac ccgcggaccc ggcggtcgat tcaaccccac
    40561 gagcccggtc agcgcgcgtg gggtccaccg ctcgctcgtc cgcgcgctcg ggatgggaca
    40621 gaccgccaag gcgctcgatc gtctgtcgac gagcgacggc tatcggttcg cgaccccgag
    40681 atcgttcggc gtggatctgc tcgcgatgcg gctcggcctc cgcttcaaca acaaggtgga
    40741 cgagtcccag gacgtgacgc cgggatcgct gctccggcgc aagcaggtcg cttggtcgct
    40801 gtaccgcgcg accacgttgg aggactgggt cgtggggtac ctcgaggatc agtacgcgga
    40861 catggtcctg cccaagatgg gcgtcggacg acgctccatc gtcgactggg gcgtccgcta
    40921 cgtcggctac ccgtacatct gggcgggcga atgggggttc gagtcgcctt caccgccggc
    40981 gttcggcggt cagccggggc cggggttcga ttgctcgggc ctgacctggt gggcgctccg
    41041 ggccgatgac ggcgtctatt gggagatctc cccgccgcgc ccccacgagg ggtgggcgct
    41101 tccccagcgc acctccgccg agatggccag gatgacgaag gtgaagctcc gctacgccga
    41161 cctgctcccg ggagacctga tgttctacga cgggaaccgc gacggcgtcg tcgatcacgt
    41221 cgacgtcttc gtaggcaacg gctggtcgct cgactcctcg agcagcgtcg gcggcgtcac
    41281 cctgatgtgg gtgaagagcg gctggtaccg ggatcacttc gtgcacggaa ggcgagtcct
    41341 tccgacgccg aagccctaga cggctccgtt cgcgtcgatc gcgctctgca gggtgttccg
    41401 ctccgcctcc gtgagctgtc ggctgcgccc ggcttccgga tccctcgcca cgatcaccga
    41461 ctccgcctcg gcggagagtc tcccatccgc cgcgtgcacg agctcacccg tccggatgct
    41521 cgaggtcccg aagctggtga tgccgcaccg gacgatcacc tctcgatcgc gctgctgcag
    41581 ctcgctccgg aagtcgatgg ccacccgcgc gaccacgaag tcccaggcct cggcaccgaa
    41641 gagggactcg acgagacgat cgcgggcctc ttcgagatag ttcaggaaca ccgcgttgtt
    41701 gacgtgaccg tacgcgtcca cgtctcgcca ccggatcggg atcctgacct ctgccgtcac
    41761 ggtcgtcacg gtaccgggtc ccggcgacgg agccggaacg tagccctcaa cgggaagggc
    41821 cggcgcgcct ccggcgcgcc ggccctccgg acctccctgg agcggtgagc tgccctagaa
    41881 gtccatgtcg ccgccgccgg gcatggcggg ggcggcttcc ttcttctcgg gcttctcggc
    41941 gaccacggcc tccgtggtga ggaacagcgc cgcgatgctg gcggcgttct gcagcgccga
    42001 gcgggtcacc ttggcggggt cgacgacgcc gaacttg
//
LOCUS       DASUYD010000189         2868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1435219, whole genome shotgun sequence.
ACCESSION   DASUYD010000189 DASUYD010000000
VERSION     DASUYD010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1435219"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VF195_10625"
     CDS             complement(<1..224)
                     /locus_tag="VF195_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581311.1"
                     /translation="MLSTRAINDQNVSRKSGWSFVRVQRESQRTHEELIATIRTLDVR
                     QWTAPVGPRGRRPLWQRLGGILSGPGGPLR"
     gene            complement(314..466)
                     /locus_tag="VF195_10630"
     CDS             complement(314..466)
                     /locus_tag="VF195_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581312.1"
                     /translation="MAEAGPLGRTQAIRILQTGRTQIDALIREIPKQALTTPGLGGGV
                     WSPRDS"
     gene            complement(466..1218)
                     /locus_tag="VF195_10635"
     CDS             complement(466..1218)
                     /locus_tag="VF195_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019067586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX6581313.1"
                     /translation="MADTAALVIEGLHKSFDGLEVLKGIDLEVREHEVVCVIGASGSG
                     KSTLLRCINLLEPVNAGRVLIEGEEITAPGVDVNRIRQRVGIVFQAFNLFPHMTVIRN
                     VTLGPIEALGTSKSEAESLARGLLERFGLADKRDDYPDRLSGGQQQRVAIVRALAMKP
                     DVLLLDEVTSALDPELVAEVLNVIRELASGGMTMVIATHEMGFARDIAHRVCFLDEGV
                     ILEEGAPEQIFTRPTQERTQRFLQSIIDAGRM"
     gene            complement(1220..2119)
                     /locus_tag="VF195_10640"
     CDS             complement(1220..2119)
                     /locus_tag="VF195_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6581314.1"
                     /translation="MIGGQSPQEFQSLPGGGATPPTGDRGSKILAPLKAEGARGVTIA
                     VVSTVVVFGLIVFAVLTSSGWPVIKEAFFDPTIFWDSLSEIMGKFGRNVFIFVVAEVF
                     VLILALILAVMRSLRGAVFTPLRILAAAYTDLFRAIPTILVILLLGFGAPSLQIRGVP
                     HSDIFWAIVALVLVYSAYVAEVYRAGIQSVHPSQVASARALGLSRGKTMRFVVVPQAV
                     RRVLPPLLNDFIGLQKDSALVAVLGYTEALRQAQIISAAEFDFTPYVALALVYIVITI
                     PEARFVDWLVARDQRRRQAAGGV"
     gene            complement(2127..>2868)
                     /locus_tag="VF195_10645"
     CDS             complement(2127..>2868)
                     /locus_tag="VF195_10645"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581315.1"
                     /translation="TLTVGTGNPAFPPWWEGGTSEGSEFEFNDPSLGEGYEGAVTGEV
                     ATRLGFSFPDQVAFVPVSFNKSFAPGDKDFDYVLQQISYKPERDDAVDFSDSYYNVNQ
                     ALVSAEGTSVADATTIEELREAKLGAPIGTTSLDVITEEIQPTQEPSVYDDLAQAVTD
                     LQNGDIDGIVVDLPTAFFMAAVQIKKGVVVGQFGAESDEYFAMAFETESPLVECVNLA
                     LDEMKADGTLDDIEQQWLADKTNAPVLD"
BASE COUNT          468 a         1010 c          947 g          443 t
ORIGIN      
        1 tgccgcaacg ggccaccggg tccgctcagg atgcccccga ggcgttgcca gaggggcctt
       61 cgcccacgcg gtccgacggg agcggtccac tgccgcacgt cgagggttct gatcgtggcg
      121 atcagctcct cgtgggtacg ctgcgactcc cgctgaacgc ggacgaagga ccagccggac
      181 ttccggctga cgttctggtc gttgatcgcc cgggtgctca gcatccgccc cagctcatcg
      241 atcggggcgc gctcccctcg ctcccaggcg acaaccgcgt cgagcgcgtg ctcctcccag
      301 ctcgcgagat gacctacgag tccctcggcg accacacccc accgccgagc cccggagtcg
      361 tcagcgcctg tttcgggatc tcgcggatga gcgcgtcgat ctgggtccgc ccggtctgca
      421 ggatccggat cgcctgagtt ctgccgagag ggccggcctc ggccatcaca tccgtccggc
      481 gtcgatgatg ctctggagga accgctgggt gcgctcctgc gtcggccgag tgaagatctg
      541 ctccggcgca ccttcctcga ggatcacccc ctcgtccagg aagcacaccc ggtgcgcgat
      601 gtcgcgggcg aaccccatct cgtgggtggc gatcaccatc gtcataccgc cggaggcgag
      661 ctcacggatc acgttcagga cctcggcgac gagctcgggg tccagggcgc tcgtgacctc
      721 gtcgaggagc aggacgtcgg gcttcatcgc gagcgctcgg acgatcgcca cgcgttgctg
      781 ctgccccccc gaaagccgat cggggtagtc gtctcgcttg tcggcgagcc cgaaccgctc
      841 gagcagcccc ctcgcgagcg actcggcttc cgacttcgac gtgccgaggg cctcgatcgg
      901 cccaagcgtg acgttgcgga tcaccgtcat gtgcgggaac aggttgaacg cctggaagac
      961 gatccccacc cgctgccgga tccggttcac atcgaccccg ggggccgtga tctcctcgcc
     1021 ctcgatcagg acccgcccgg cgttcacggg ttcgagcagg ttgatacagc ggagcagggt
     1081 cgacttgccc gagcccgacg cgccgatcac gcagacgacc tcgtgttcac gcacctcgag
     1141 gtcgatgccc ttgaggacct cgagcccgtc gaacgacttg tgcaggccct cgatcacgag
     1201 cgcggctgta tcggccacct cacacccccc cggcggcctg gcgccggcgc tggtcccggg
     1261 cgaccaacca atcgacgaac cgggcctcgg ggatcgtgat cacgatgtac acgagcgcga
     1321 gcgcgacgta cggcgtgaag tcgaactcgg cggccgagat gatctgggcc tggcggagcg
     1381 cctccgtgta cccgaggacg gccaccagcg ccgagtcctt ctgcaggccg atgaagtcgt
     1441 tgagcagcgg tggcagcacg cggcggacgg cctgcggcac gacgacgaac cgcatggtct
     1501 tgccgcggct gagaccgagc gcacgtgcgg acgcgacctg cgaagggtgg acggactgga
     1561 tccccgcccg gtagacctcg gcgacgtagg ccgagtagac gaggacgagc gccacgatcg
     1621 cccagaagat gtctgagtgc ggcacgcccc tgatctgcag cgacggcgcc ccgaacccga
     1681 gcagcaggat caccaggatg gtcgggatcg cgcggaacag gtccgtgtac gccgccgcca
     1741 ggatgcggag cggggtgaac accgcgcccc gcaggctccg catcacggcg aggatcaggg
     1801 ccaggatcag gacgaagacc tcggccacca cgaagatgaa cacgttccgg ccgaacttcc
     1861 ccatgatctc ggagagcgag tcccagaaga tcgtcgggtc gaagaacgcc tccttgatga
     1921 ccggccaacc cgaggacgtc aggaccgcga acacgatcag cccgaagacg acgacggtgc
     1981 tgaccaccgc gatcgtgacc ccacgcgcgc cctcggcctt cagcggcgcg aggatcttcg
     2041 aaccgcggtc accggtcgga ggcgtggccc cgccgcctgg caagctctgg aactcctgcg
     2101 gcgactgccc gccgatcacg gaggggctag tccaggacgg gcgcgttcgt cttgtcggcc
     2161 agccactgct gctcgatgtc gtcgagcgtc ccgtccgcct tcatctcgtc gagcgcgagg
     2221 ttcacgcact cgaccagcgg gctctcggtc tcgaacgcca tcgcgaagta ctcgtccgac
     2281 tcggctccga actgccccac gacgacgccc ttcttgatct gcaccgccgc catgaagaac
     2341 gcggtcggca ggtcgacgac gatgccgtcg atgtcgccgt tctggaggtc ggtgacggct
     2401 tgcgcgaggt cgtcgtagac cgagggctcc tgcgtcggct ggatctcctc ggtgatgacg
     2461 tccaggctcg tcgtcccgat cggcgcgccg agcttcgcct ccctcagctc ctcgatcgtg
     2521 gtcgcgtcgg cgaccgacgt accttcggcc gagaccagcg cctggttcac gttgtagtac
     2581 gagtcgctga agtccacggc gtcgtcgcgc tcgggcttgt acgagatctg ttggagcacg
     2641 tagtcgaagt ccttgtcccc gggtgcgaac gacttgttga acgacacggg cacgaacgcc
     2701 acctggtcgg ggaaggagaa gcccagccgg gtcgccacct cgccggtcac cgcgccctcg
     2761 tagccctcgc cgagcgacgg atcgttgaac tcgaactcgg aaccctcgga ggtgccgccc
     2821 tcccaccagg gcgggaaggc cgggttcccc gtgccgaccg tcagcgtg
//
LOCUS       DASUYD010000190         3822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_555572, whole genome shotgun sequence.
ACCESSION   DASUYD010000190 DASUYD010000000
VERSION     DASUYD010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555572"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /gene="nadD"
                     /locus_tag="VF195_10650"
     CDS             complement(<1..515)
                     /gene="nadD"
                     /locus_tag="VF195_10650"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009947731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEX6581316.1"
                     /translation="MGGTFDPIHYGHLVTAEEALEQFELEGVLFVPTGRPWMKEHEVV
                     SPPEDRYLMTVIATASNPLFSVSRIEVDRDGPTYTVETLRSLKADLDADLFFVTGADA
                     ILEMFQWKEPEELFELAHFIAATRPGYDIAGFEVPAHAKDAVSVMNIPALAISSTDIR
                     GRVQAGGPIRYL"
     gene            complement(546..1004)
                     /locus_tag="VF195_10655"
     CDS             complement(546..1004)
                     /locus_tag="VF195_10655"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6581317.1"
                     /translation="MSRSPNIDELLVDEDRLWLELTEVFGDVSPDRFDEPSVTPEGWS
                     PKDVMYHVAAWADEAATVLGRIAAGTHTGGDPDTQALNEEWFEVGRGLDDDVVRIRFA
                     KGRTAMRQAFTRLSEVDAAAWEWFEESGPRHYEEHLPDLRAFLERGTPRP"
     gene            complement(1001..2275)
                     /gene="obgE"
                     /locus_tag="VF195_10660"
     CDS             complement(1001..2275)
                     /gene="obgE"
                     /locus_tag="VF195_10660"
                     /inference="COORDINATES: protein motif:HMM:NF008954.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX6581318.1"
                     /translation="MTFVDEVDVEVRAGRGGDGSAAMRSEPYKPKGGPEGGDGGRGGD
                     VVFRATRNLHDLAWFADHPHLRAEPGTPGARSRREGRNGKDLVVPVPDGTVVFDEQGL
                     VADLVGEGAEAVVATGGRGGRGNTRFASPKNRVPRTAEAGEEGEERRLHVELRTVADL
                     GLVGLPNAGKSTLLAKLTAATPKIADYPFTTLTPNLGVAEAGDERFIVADIPGLVEGA
                     HEGRGLGHRFLRHITRCRALVIVVDLSSPDAPSDLETVRAELRAYDPELAERPSLVVG
                     TKVDLVEDPARAADAIRGLPVSAVTGEGLELLATQLEQLSRQGAAQLPPRAPYVVLRP
                     GRARFTISKERGGWRVVGRNVERWVHEADLDDERALVALQRKLIKEGVERQLAKAGAR
                     RGDEIRISDRTFEFLPEVDRPEVDPGEDRREA"
     gene            2326..2775
                     /locus_tag="VF195_10665"
     CDS             2326..2775
                     /locus_tag="VF195_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581319.1"
                     /translation="MVEPVTVWMVHLGLATGTKPPEVRGTLTLGDEGLEFVERKTGAD
                     VLFDYASIRRAKRLRGSPVLLLDWRTGEANRKTAFYFSQPPPLEPVARSATIDPRGPL
                     GPFTRTTPSKRQVARMNLGYLRSTSVTNRQVVKAWAEAVSERMRRAG"
     gene            complement(2772..3026)
                     /gene="rpmA"
                     /locus_tag="VF195_10670"
     CDS             complement(2772..3026)
                     /gene="rpmA"
                     /locus_tag="VF195_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX6581320.1"
                     /translation="MAHKKGGGSSRNGRDSTSKRLGVKVFGGEAVAAGSIIVRQRGTR
                     IHPGDGVAKGGDDTLFALRDGTIEFHESRGRKYASVLADA"
     gene            complement(3046..3402)
                     /gene="rplU"
                     /locus_tag="VF195_10675"
     CDS             complement(3046..3402)
                     /gene="rplU"
                     /locus_tag="VF195_10675"
                     /inference="COORDINATES: protein motif:HMM:NF013025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX6581321.1"
                     /translation="MYAVIKTGGKQHRVQVGDVIEIEKLVVDGETVEFHPLLLVDDDG
                     KTHVGTEAAKAVVIAKPLGEKKGEKVKVFKYRPKSGYARKAGHRQLLTMLEITEVKLP
                     AKRAAATKKDAAAASE"
     gene            complement(3441..>3822)
                     /locus_tag="VF195_10680"
     CDS             complement(3441..>3822)
                     /locus_tag="VF195_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019548225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX6581322.1"
                     /translation="EQTEAMTIVDVNTGKAVGKTNLEETVLTTNLEAAKEVARQLRLR
                     DIGGIIVIDFIDLLLEQNKRKVEDTMRETLAIDKTRSQVFEIGPLGIMQITRKRVSGG
                     LVDSFSETCPQCEGRGIIVNYDAS"
BASE COUNT          538 a         1402 c         1241 g          641 t
ORIGIN      
        1 aggtaccgga tcgggccgcc ggcctgcacg cggccgcgga tgtcggtcga ggagatcgcg
       61 agcgcgggga tgttcatcac gctcaccgcg tccttcgcat gcgccggcac ctcgaacccc
      121 gcgatgtcgt acccgggacg ggtcgccgcg atgaagtgcg cgagctcgaa gagctcctcc
      181 ggctccttcc actggaacat ctcgaggatc gcgtcggcgc ctgtcacgaa gaacaggtcc
      241 gcgtccaggt cggccttcag cgagcggagg gtctccacgg tgtacgtggg cccgtcgcgg
      301 tcgacctcga tccgcgagac ggagaacaaa gggttgctcg ccgtcgcgat cacggtcatc
      361 aggtagcggt cttccggcgg cgagacgacc tcgtgctcct tcatccacgg ccggccggtc
      421 gggacgaaga ggacgccctc gagctcgaac tgctcgagcg cttcctctgc cgtcacgagg
      481 tggccgtaat ggatcggatc gaacgtcccg cccatcaccc ccaggcgtcg ccccacgttc
      541 gaatcctacg gccggggggt gcctcgctcc aggaacgcgc gcagatcggg caggtgctcc
      601 tcgtagtgac gtggtccgga ttcctcgaac cactcccagg cggccgcgtc gacctccgag
      661 agcctcgtga aggcctgtcg catcgccgtt cgccccttcg cgaagcggat ccgcacgacg
      721 tcgtcgtcga ggccacgccc cacctcgaac cactcctcgt tgagggcctg ggtatccggg
      781 tcgccgcccg tgtgcgtgcc ggcggcgatc cgcccgagga cggtggccgc ctcgtccgcc
      841 cacgccgcca cgtgatacat cacgtccttc ggcgaccatc cttcgggcgt gaccgagggc
      901 tcgtcgaacc gatcgggcga cacgtcaccg aagacctccg tgagctcgag ccagagccga
      961 tcctcgtcga ccaggagctc gtcgatgttc gggctccggc tcacgcctcc cgccgatcct
     1021 ctccgggatc cacctccggc cgatcgacct caggaaggaa ctcgaacgtg cgatccgaga
     1081 tccggatctc gtcgccgcga cgcgcccccg ccttcgcgag ctgtcgttcg acgccttcct
     1141 tgatcagttt ccgctggagg gccacgagcg cccgttcgtc gtcgagatcc gcctcgtgca
     1201 cccaccgctc cacgttccgc ccgacgaccc gccaccctcc tcgctctttc gagatcgtga
     1261 accgcgcacg cccgggacgg agcaccacgt acggggcacg aggagggagc tgcgcggccc
     1321 cctgtcgcga cagctgctcg agctgcgtcg cgaggagctc gaggccctcg ccggtcacgg
     1381 ccgacaccgg caggccgcgg atcgcgtcgg ccgctcgcgc cggatcctcg acgaggtcca
     1441 ccttcgtccc caccacgagc gacgggcgct cggcgagctc cggatcgtac gcacggagct
     1501 ccgcgcggac ggtctccagg tcggacggcg cgtccgggga cgagaggtcc acgacgatga
     1561 cgagcgctcg gcatcgggtg atgtgccgga ggaaccggtg gccgagccct ctgccctcgt
     1621 gggctccttc gaccaacccg gggatatcgg ccacgatgaa ccgttcgtcg cccgcctccg
     1681 cgacgcccag gttgggcgtg agcgtggtga acggatagtc cgcgatcttc ggggtggcgg
     1741 cggtcagctt cgcgagcagc gtcgacttcc ccgcgttcgg gagacccacg aggccgaggt
     1801 cggccaccgt ccgcagctcc acgtgcaggc gccgctcctc gccctcctcg ccggcctcgg
     1861 cggtcctcgg cacgcggttc ttgggactcg cgaagcgtgt gttgccgcgc ccaccgcgcc
     1921 ctcccgtggc caccacggcc tcggcgccct cgccgaccag gtcggcgacc aagccctgtt
     1981 cgtcgaagac caccgtgccg tccggcacgg gaaccacgag atccttgccg ttcctgccct
     2041 cgcggcggga ccgggctccg ggggtgcccg gctcggcccg caggtgcggg tggtccgcga
     2101 accacgcgag gtcgtgcagg ttccgggtcg cgcggaacac cacatcgccg ccgcggccac
     2161 cgtcgccgcc ttccggccca cccttcggct tgtagggctc gctgcgcatc gcggcggacc
     2221 cgtcaccacc gcggccggca cgcacctcca cgtcgacctc gtccacgaac gtcatgccgg
     2281 acagcctagg tggtcgacgg cgccgccagc cgcgaagatt gccgcgtggt cgagcccgtc
     2341 accgtctgga tggtccatct cggtctcgcg accgggacga agccgcccga ggtgcggggg
     2401 acactcaccc tcggcgatga aggactggag ttcgtcgaac gcaagaccgg ggcggacgtt
     2461 ctcttcgact acgcatcgat ccgccgcgcg aagcgcctcc gaggctctcc cgtgctcctg
     2521 ctggactggc gcacgggtga ggcgaacagg aagacggcct tctacttctc gcagccacct
     2581 ccgctggaac cggtcgcccg ctccgcgacc atcgatccac gggggccgct cggaccgttc
     2641 acgcgaacga cgccgtcgaa gcgtcaggtc gcgaggatga acctcgggta tctccgctcc
     2701 acgagcgtca cgaacaggca ggtcgtgaag gcgtgggcgg aggcggtgtc cgagcggatg
     2761 cggcgagcgg gctaggcgtc ggcgagcacg gacgcgtact tccggccccg tgactcgtgg
     2821 aactcgatcg tgccgtcccg gagggcgaac agcgtgtcgt ccccgccctt ggcgacgccg
     2881 tcaccggggt ggatccgcgt cccgcgttga cgcacgatga tcgagccggc ggcgaccgct
     2941 tcaccgccga acaccttgac ccccagtcgc ttcgaggtcg agtcccgacc gttccgggac
     3001 gagccgccgc ccttcttgtg tgccatggtc caacgaggct accagctact cggaggccgc
     3061 cgcggcatcc ttcttcgtgg cggccgcccg cttcgccggc agcttcacct cggtgatctc
     3121 gagcatcgtg agcagctggc ggtgcccggc cttgcgggcg tacccggact tcggccggta
     3181 cttgaagacc ttgaccttct cgcccttctt ctcgcccagg ggcttggcga tcacgaccgc
     3241 cttggcggcc tcggtcccga cgtgcgtctt gccgtcatcg tcgacgagga gcaacgggtg
     3301 gaactccacc gtctccccgt cgacgaccag tttctcgatc tcgatcacgt cgccgacctg
     3361 caccctgtgt tgcttgccgc cggtcttgat caccgcgtac atggctcgtt cctctctggc
     3421 atggtcctcg gtcgtggtcg tcagctcgcg tcgtagttca cgatgatgcc gcgcccctcg
     3481 cactgggggc aggtctccga gaaggagtcc accaggccgc ccgagacgcg cttgcgcgtg
     3541 atctgcatga tccccagcgg gccgatctcg aacacctggg atctggtctt gtcgatcgcg
     3601 agggtctctc gcatcgtgtc ctcgaccttc cgcttgttct gctccaggag cagatcgatg
     3661 aagtcgatca cgatgatgcc accgatgtcc cggagacgga gctgtcgcgc gacctccttc
     3721 gccgcttcca ggttcgtcgt gaggaccgtc tcctcgaggt tcgtcttgcc gacggccttc
     3781 ccggtgttga cgtcgacgat cgtcatggcc tctgtctgct cg
//
LOCUS       DASUYD010000191         6084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_999038, whole genome shotgun sequence.
ACCESSION   DASUYD010000191 DASUYD010000000
VERSION     DASUYD010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6084
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_999038"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..366)
                     /locus_tag="VF195_10685"
     CDS             complement(7..366)
                     /locus_tag="VF195_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015339055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581323.1"
                     /translation="MGGVRVGSVVIDCNDFDRMFAFWRDALGYVPRDEPEDDWVVLRD
                     PSGSNVNVSLQVVPEPRVGKNRLHLDLYAEHRDAEIERIVGLGATIHPRTPEPDEDFV
                     VLADPEGNLFCVIQKDG"
     gene            complement(407..2581)
                     /locus_tag="VF195_10690"
     CDS             complement(407..2581)
                     /locus_tag="VF195_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6581324.1"
                     /translation="MFPLAAGLIIGAVEVVLAVAFAALVFAGSTPARLGDGIGLYLVA
                     AALTLGFLAWRAGMRGVVGSVQDAATAVLSILALSTATKAINIMRICQTASVGRCEVP
                     DVFLTVVAATLVVTLSCGAIFLVLGTFRLGNLVRFIPYPVVGGFLAGTGWLLFKGGIY
                     IASGVELHLRTFQLLVSPVTLKRWVPALAFGVILLLAVRRVKKPLVIPIVLAIGLVLF
                     AIGMLVTGSSIDAAQHGRWLLGPFESARLWQPWTLRALTGADWSAVLSQWAGILTAVF
                     VATLAILFNISGTEVVLHRDLDTNRELRDAGALNVVSGALGGIPGYHALSLSALAERM
                     KVNARAAGLFAALVPLAAVVFGAAFIELIPRMIVGGVLIFLGLAFIVEWVWDMRRILP
                     PFEYAIVLVILATIIGRDFLTGVVVGLVLAGILFAVNYGRIELVREVVFGETYHSNVD
                     RPPGERAALEAMRHRVQILRVNGFMFFGSANGLLERIRTRMEATPLRFLVIDLRRVTG
                     VDSSAVVAFVKVMHLAKANGFELVFTGASDEVRTQLGQGGVVESEGIVRFEPDLDRGL
                     QRCEEGLLEGAAADPPVVVDGSSDGLAGMPPGLRAYLERRELPEGTVLIRQDEQPDDV
                     FVLESGRLSVETVTPEGTRLRLRTLRPGVVVGEMAMYTGVLRSADVVAETPSVVLRLS
                     RESIERVEAADPALAAALHRWLATTLSERLGESLRAFDALLD"
     gene            complement(2633..4858)
                     /locus_tag="VF195_10695"
     CDS             complement(2633..4858)
                     /locus_tag="VF195_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEX6581325.1"
                     /translation="MSEQPRTAGWPTTIAAGVIIGAVEAFLAVSFAALVFGGYLEDFI
                     PDGIALYLGAAALTLAIFAWRAGTRGVVGGMQAATAAVIAIVAASTAVGTFGSPERAF
                     LTVVAATLAVTVLSGAIFLVLGMLRRGNLIRFVPYPVVGGFLAGAGWLLLKGGIYVAS
                     GESPSTSTIGDLTDSTVLQLWLPALAFGVILLIATRLVKRPLVIPAVLGIGLVLFVIG
                     MLVTGSSIESARGGNWLLLSVFDEAQPWEPWTLRAVTGADWLAVLEQWAGIAAAVFIA
                     VVVILFNISETELVLHRDLDTNQELRDAGVLNVVSGAVGGIPAYHALNMSGLGEDMKV
                     NARRAGLIAALVPLAAVLFGASVIELIPRMIVGGVLVFLGLAYIVEWVWDRRRSLPAV
                     EFGVVLVILAAFIVRGLIPGVVIGLVLAIVLFAVSYGRIELVREVAFGDTYHSNVDRP
                     PGQRAVLRSMGDRVHILRVNGFVFFGSANSLLERIRKRVEAGPPRFLVVDLRRVTGVD
                     SSAVMSFVKVVHLAQSNGFELVFTGASDPVRKQLERGGVVEAEGIVRFEPDLDRGLQL
                     SEEGLLRVARPDGAEVEDDAARDGAVDAAAERVTAPDGSSAALAGMPAGLGTHLERVT
                     LSEGTVLIRQDEPPDDVFVLEEGRLSVEMVTGEGTRLRLRSVNPGVVVGEVSMYTGLP
                     RTADVVAETPSVVLRLSGATIRRIEADEPELAAAMHRWLARTLSERLTDTLREFDALL
                     D"
     gene            complement(4955..5479)
                     /locus_tag="VF195_10700"
     CDS             complement(4955..5479)
                     /locus_tag="VF195_10700"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581326.1"
                     /translation="MARRFRPLRTQTSTTSKASPEVVYDTIADLRAHLEWSGERASSD
                     TFKLLELDAPAGTATVGTTFISSGAADVGTFHDRSVVREASRPTRFTIETDSRLDRKR
                     GEAWEAYFSHRYDVEPDGAGSKIVYTETIERANYTPYWLNALARPIFRPLVNRADRKQ
                     LQNLARLAEERSNG"
     gene            complement(5487..5735)
                     /locus_tag="VF195_10705"
     CDS             complement(5487..5735)
                     /locus_tag="VF195_10705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581327.1"
                     /translation="MDAVVWTAIGLLAATSLGTLFFLGARIDGLGSRIDGFGSRIDGL
                     GSRIDGLSARMDSRFDHMDERFDALAARLDAHLDRHTG"
     gene            5864..>6084
                     /locus_tag="VF195_10710"
     CDS             5864..>6084
                     /locus_tag="VF195_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581328.1"
                     /translation="MANTVPPDGPSPEKAAQDVQIRTFLIADVRGYTLVTQERGDEAA
                     AKLAAKFADIAREVIEERGGTLLELRGDEA"
BASE COUNT         1073 a         2177 c         2015 g          819 t
ORIGIN      
        1 cctgcctcag ccgtccttct ggatgacgca gaacaggttg ccctcgggat ccgcgaggac
       61 gacgaagtcc tcgtcgggct ccggggtccg ggggtggatc gtcgcaccga gcccaacgat
      121 ccgctcgatc tcggcgtccc ggtgctccgc gtagagatcg agatggagcc ggttcttccc
      181 gaccctgggc tcgggaacga cctggagcga gacgttcacg ttcgaacccg aggggtcgcg
      241 cagcacgacc cagtcgtcct ccggttcgtc acgcgggacg tagcccagcg cgtcccgcca
      301 gaacgcgaac atgcggtcga agtcgttgca gtcgatgacg accgatccga cccgaacacc
      361 acccatgctc gcgctcctct cggatgagcg gtgaggatag cgagtcctag tccaacaagg
      421 cgtcgaaggc cctcaacgac tcgcccaacc gctccgacag cgtcgtggcg agccaccggt
      481 gcagcgctgc agcgagcgcg ggatccgcgg cctccacccg ctcgatggac tccctgctga
      541 gccgcaggac cacgctcggc gtctcggcca cgacgtcggc gctgcgcagg acccccgtgt
      601 acatcgccat ctcgccgacg accacaccgg ggcgcagcgt ccgcagtcgc aaccgtgtcc
      661 cctccggtgt cacggtctcg acgctcagcc ggcccgattc caacacgaac acgtcgtcgg
      721 gctgttcgtc ctgacggatc aggacggtcc cctcggggag ctccctgcgc tcgaggtacg
      781 cccgcaggcc gggcggcatg ccggcgagcc catcgctcga cccgtccacg acgactgggg
      841 ggtcggcggc ggcgccctcg agcagaccct cctcgcatcg ctgcaggccg cggtccaggt
      901 ccggctcgaa ccgtacgatc ccctcggact cgacgacccc gccctgccca agttgcgtcc
      961 gcacctcatc ggacgcgccc gtgaacacga gctcgaaccc gttcgccttt gccaggtgca
     1021 tcaccttcac gaacgccact accgccgacg agtccacgcc cgtcacgcgc cggagatcga
     1081 tcaccaggaa ccgcaacggt gtcgcttcca tgcgcgtgcg gatccgctcg agcaggccgt
     1141 tggccgatcc gaagaacatg aacccgttga cgcgcaggat ctgcacacgg tgacgcatcg
     1201 cctccaacgc cgcgcgctcg ccgggcggcc gatcgacgtt gctgtggtac gtctccccga
     1261 agaccacctc acgcaccagc tcgatccggc cgtagttgac cgcgaacagg atgcccgcga
     1321 gcacgagccc gaccacgaca ccggtcagga agtcccgccc gatgatcgtc gcgaggatca
     1381 ccaggacgat ggcgtactcg aacggcggca ggatccgccg catgtcccac acccactcga
     1441 cgatgaaggc gaggccgagg aagatcagga caccgccgac gatcatccgc gggatcagct
     1501 cgatgaacgc cgccccgaac acgaccgccg cgagcgggac gagcgcggcg aagagcccgg
     1561 cggcccgcgc gttcaccttc atgcgttcgg ccagcgcgct caggctgagc gcgtggtacc
     1621 cggggatccc acccaacgcg ccggagacca cgttgagcgc cccggcgtcg cggagctccc
     1681 ggttggtgtc caggtcccgg tgcagcacga cctcggtccc cgagatgttg aacaggatcg
     1741 cgagcgttgc cacgaagacg gcggtcagga tcccggccca ctggctcagc accgccgacc
     1801 agtcggcacc ggtgagtgcc cggagcgtcc acggctgcca cagccgggcc gactcgaacg
     1861 gcccgagcag ccatcgccca tgctgagcgg catcgatcga agagcccgtc acgagcatgc
     1921 cgatcgcgaa cagcacgagc ccgatcgcga gcacgatcgg gatgaccaag ggtttcttca
     1981 cccgccgtac ggcgaggagc aggatcacgc cgaaggcgag cgcgggcacc catcgcttga
     2041 gcgtcaccgg gctcaccagg agctggaacg tgcgaaggtg cagctccaca ccggaggcga
     2101 tgtagatgcc gcccttgaag agcagccatc cggtgcccgc caggaacccc ccgacgaccg
     2161 gatacgggat gaaccggacc aggttgccga gccggaaggt cccgagcacg aggaagatgg
     2221 caccgcacga caacgtcacc accagcgtcg cggccacgac ggtgaggaag acgtcgggaa
     2281 cctcgcacct gccgacggac gcggtctggc agatcctcat gatgttgatg gccttggtcg
     2341 ccgtgctcag cgccaggatc gagagcacgg ccgtggcggc gtcctgcacg ctgcccacga
     2401 cgccgcgcat gcccgccctc cacgccagga acccgagcgt gagcgcggcc gcgacgaggt
     2461 agaggccgat gccatcgcca aggcgggcgg gggtcgagcc ggcgaacacg agggccgcga
     2521 acgcaaccgc gagcaccacc tccacggcgc cgatgatcaa gcctgcggcc agcgggaaca
     2581 tccgtcccac cgatcgtcgt tgctcggaca cgctcctccc tacctccctg gcctagtcga
     2641 gcagagcgtc gaactccctc aacgtgtcgg tcaaccgctc cgacagggtc ctcgcgagcc
     2701 accggtgcat cgccgcggcc agctcgggct cgtcggcttc tatccgccgg atcgtcgctc
     2761 cgctgagccg caggaccacg ctcggggtct cggccaccac atcggccgtg cgcgggagcc
     2821 ccgtgtacat cgagacctcg ccgacgacga cgccggggtt caccgatcgc agacgcaggc
     2881 gggtcccctc gcccgtcacc atctccacgc tcagacgacc ctcttccaag acgaagacgt
     2941 cgtccggtgg ctcgtcctgt cggatcagca cggttccctc cgagagcgtg acgcgctcga
     3001 ggtgcgtccc cagacccgcc ggcatgccgg cgagcgcggc gctcgacccg tcgggggccg
     3061 tcactcgttc ggcagcggca tcgacggcgc cgtcccgggc agcgtcgtcc tcgacctcgg
     3121 ctccgtcggg cctcgcgacc cggagtagac cttcctcgga caactggagc ccgcggtcca
     3181 ggtccggctc gaaccggacg atcccctcgg cctcgacgac cccgccgcgc tcgagttgct
     3241 tgcggaccgg atccgacgca cccgtgaaca cgagctcgaa gccgttcgac tgggccaggt
     3301 gcaccacctt gacgaacgac atgaccgcgg aggaatccac gcccgtcacg cggcgaagat
     3361 cgacgaccaa gaaccgcggc ggcccggcct caacgcgctt gcggatccgc tcgagcaggc
     3421 tgttggccga tccgaagaac acgaacccgt tcacgcggag gatgtggacg cgatcgccca
     3481 tcgagcgcag caccgcccgt tgaccgggcg ggcggtcgac gttgctgtgg tacgtatctc
     3541 cgaacgccac ctcgcgcacc agctcgatcc ggccgtaact gaccgcgaac aggacgatcg
     3601 caagcaccag gccgatcacg acgccgggta tgagtccccg cacgatgaac gccgcgagga
     3661 tcacgaggac gactccgaac tcgacggccg gcagggaccg ccgccggtcc cacacccact
     3721 cgacgatgta ggcgagcccg aggaacacca gaactcctcc gacgatcatg cgcgggatca
     3781 gctcgatgac ggacgcgccg aacaggaccg cggcgagcgg gacgagcgcg gcgatgagcc
     3841 ccgcccggcg cgcgttgacc ttcatgtcct cgccgagccc gctcatgttg agggcgtggt
     3901 acgcggggat cccgccgacg gccccggaga cgacgttgag gacgccggcg tcgcggagct
     3961 cttggttcgt gtcgaggtcc cggtgcaaga cgagctcggt ctcgctgatg ttgaacagga
     4021 tcacgaccac ggcgatgaac accgcggcgg cgatccccgc ccactgttcg agcaccgcca
     4081 gccagtcggc gccggtcacc gctcgcaggg tccacggctc ccacggttgg gcctcgtcga
     4141 agacgctgag caggagccag ttgccccctc gcgcgctctc gatcgaggag ccggtgacga
     4201 gcatgccgat cacgaacagc acgagcccga tcccgaggac cgccgggatc acgagcggcc
     4261 gcttcaccaa ccgggtggcg atgagcagga tcacgccgaa ggcgagggcc ggaagccaga
     4321 gctgcagcac ggtggaatcc gtcaggtcgc cgatcgtgct cgtggacggc gactcacccg
     4381 acgcgacgta gatgccgccc ttcaggagca gccacccggc acccgcgagg aaccccccga
     4441 cgaccggata cggcacgaag cggatcaggt tgccccgccg gagcatcccg agcacgagga
     4501 agatcgcgcc gctgagaacg gtcaccgcca aggtggcggc gacgacggtg aggaaggcgc
     4561 gttcggggct tccgaacgtg cccacggccg tcgatgcggc gacgatggcg atcacggccg
     4621 cggtcgccgc ctgcatgccg ccaacgactc cgcgcgtgcc cgccctccag gcgaagatcg
     4681 cgagcgtcaa cgcggccgct ccgagataca gagcgatgcc gtcggggatg aaatcctcga
     4741 ggtagccgcc gaacacgagt gcggcgaagg agaccgcgag gaacgcctcg actgcgccga
     4801 tgatcacgcc ggcggcgatg gtcgtcggcc atcctgcggt tcgcggctgc tcggacacgc
     4861 ccctccctcc gggcccggac cgttggagaa ggactttatg ccggacggcg tccgggcgtg
     4921 caccgcgacg cgtcggggcc ctggtgagct gtggtcagcc gttcgagcgt tcctcggcga
     4981 gccgcgcgag gttctggagc tgcttccggt cggcccggtt gacgagcggg cggaagatcg
     5041 gccgcgccag tgcgttgagc cagtagggcg tgtagttcgc gcgttcgatc gtctccgtgt
     5101 acacgatctt ggagccggcc ccgtcgggtt cgacgtcgta ccgatgggag aagtacgcct
     5161 cccaggcctc gccgcgcttg cggtcgaggc gagagtccgt ctcgatggtg aaccgggtcg
     5221 gccgcgaagc ctccctgacg accgagcgat cgtggaacgt gccgacgtcc gcggcaccgc
     5281 tcgagatgaa cgtcgtgccc acggtcgccg tccccgccgg tgcatcgagt tcgagcagct
     5341 tgaacgtgtc gctgctcgcg cgttcgccgg accattccag gtgcgctcgc aggtcggcga
     5401 tcgtgtcgta cacgacctcc ggcgacgcct tcgaggttgt ggacgtctgg gttcggagcg
     5461 gacggaaacg ccgtgccatc gccgattcag cccgtgtggc ggtcgagatg agcgtcgagg
     5521 cgggcggcga gtgcgtcgaa ccgctcgtcc atgtgatcga agcgcgagtc catgcgggcg
     5581 gagagcccat cgatccgtga gccaaggcca tcgatgcgtg agccgaaccc atcgatccgc
     5641 gagccgagcc catcgatccg tgcgccgaga aagaagagcg tcccgagaga cgtcgcggcg
     5701 agaaggccga tcgcggtcca aacgacggcg tccatctcgc tcgcgagcgt agcactcggt
     5761 tacgacgcag cggcctgcgc gtctctcggc gacggccgat cctgaaatcg atatcgggga
     5821 ccggagcttc cgctcggacc agcgaaacga cggataatgc cgcgtggcaa acacggtccc
     5881 ccccgacggt ccgagccccg agaaagcagc gcaggacgtt cagatcagga cgttcctgat
     5941 cgccgatgtc cgcggctaca ccctcgtcac ccaggagcga ggtgacgagg ccgccgcgaa
     6001 gctcgccgcg aagttcgccg acatcgcgcg agaggtcatc gaggaacgcg gtggcacgct
     6061 ccttgagctc cgcggggacg aggc
//
LOCUS       DASUYD010000192         3497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1222052, whole genome shotgun sequence.
ACCESSION   DASUYD010000192 DASUYD010000000
VERSION     DASUYD010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222052"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VF195_10715"
     CDS             <1..583
                     /locus_tag="VF195_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEX6581329.1"
                     /translation="YHPGEANVRMADIVVVNKVGTATADQVIAVESSIHELNPQATIV
                     RADSVVSLTDPELVRGRRVLVVEDGPTLTHGGMKFGAGVVAARRAGAAEILDPRPFVT
                     GTIAEVFEKYDVGPVLPAEGYSPEQLGELEQAIAATPCDTIVIGTPMDLRHVIRLTKP
                     AVRVTYELEEIGPPMFPGLLRTAITNARIGGLT"
     gene            complement(1058..1195)
                     /locus_tag="VF195_10720"
     CDS             complement(1058..1195)
                     /locus_tag="VF195_10720"
                     /inference="COORDINATES: protein motif:HMM:NF020512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX6581330.1"
                     /translation="MNRFGKEERWGASVGDRLVIRGHHVGEQDRDGEIVEVRGGAGGP
                     P"
     gene            complement(1215..1529)
                     /locus_tag="VF195_10725"
     CDS             complement(1215..1529)
                     /locus_tag="VF195_10725"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6581331.1"
                     /translation="MVLHVCEREYVYGVDVSLEQLEDANDLAERHFRRMNDAGRSARG
                     EVVSAAYGRRARGILGLAEDERVDLIVMGTRGLSVWSRLIVGSVAHRVLHSARVPVLV
                     VR"
     gene            complement(1648..2250)
                     /locus_tag="VF195_10730"
     CDS             complement(1648..2250)
                     /locus_tag="VF195_10730"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6581332.1"
                     /translation="MDSSEIFIIVALAWSLLGVSMALVMGRRGHQPFMWLVLGITFGP
                     LVLPLAANALLHDRPGLLKRLSPARPSTGGVDVLVGIDGSAEAEDALRTAIGLLGPRL
                     GRLTLAAVVDYDVAVGSGLAEDNERARGDLIRTANATALRPETVLLSGRPAEALRKFA
                     VEEGYALIVVGRRGRGASHVLLGSVASKLAHGGDVPVLIV"
     gene            complement(2532..>3497)
                     /locus_tag="VF195_10735"
     CDS             complement(2532..>3497)
                     /locus_tag="VF195_10735"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX6581333.1"
                     /translation="LILPFAVFVMALFFLVAGLLTPASLERKGPGRFARDRLIRLGIP
                     LAAFMFVLWPLLTLGLYRAAGSDWSLWDVYKDEPTLDNGPLWFVEVLLIYSLVYAAWV
                     GWRTRQGRTPNAVRAGPLRARHLVGLGAGIVIATFVVRRWFPFDSLQFTNAHVWQWPE
                     CIGLFGLGIVSARRGWLRPVPERIRRGCGWVALLATVALIVAFASSQDPDRSVEAFGG
                     GWRWEALVLATIGGAMSVAASVWVLAFAQQHWDRAGRTARAMGRAAYAAFMFQGFPLV
                     VAALALRPLNGPLEVKAVLVAAAGIAGSFALGWLLVTRTRLGRIM"
BASE COUNT          647 a         1252 c         1137 g          461 t
ORIGIN      
        1 gtaccacccg ggcgaagcca acgttcggat ggccgacatc gtagtggtga acaaggtcgg
       61 taccgccacc gccgaccagg tgatcgcggt ggagtcgtcg atccatgagc tcaaccctca
      121 ggcgacgatt gtccgagccg acagcgtggt cagcctgacc gaccccgagc tggtccgcgg
      181 gcgtcgcgtg ctggtcgtcg aagacggacc gaccctcacg cacggcggga tgaagttcgg
      241 cgccggcgtg gtggccgccc gaagagcggg cgctgccgag atcctcgacc cgcgaccctt
      301 cgtcacgggg accatcgcgg aggtcttcga gaagtacgac gtcgggccgg tcttgcccgc
      361 agagggttac tcccccgaac aattgggaga gctcgagcaa gccatcgcgg cgacaccatg
      421 cgacacgatc gtgatcggca cgccgatgga ccttcggcac gtgatcagac tgacgaagcc
      481 ggcggtccgc gtgacctacg agctcgagga gatcggccca ccgatgtttc cgggtctcct
      541 acgaacggcg atcacgaacg cgcgcatcgg gggcctcacg tgatggtcga acgagtcgcc
      601 ccgcttcgcc ccacgcgggg cggggcggct cccggttcga ccctcttcgc aggatcccca
      661 catggatact gcagatgagg cgccgagacg atctcgttgg gggacgatga ccacccaagg
      721 gatgggccgc gctggatatc ccggtgcgcc tcagggaacc aggatccggg tgcgcatcga
      781 ggcgcattca cgacgctgca agccgaacgc acaatcgaga tcgagttccc cggcgcgccc
      841 ggcccgattg gcgttgggcc atggggcgtg ggcacccgtc gggacacggc cggtgggagg
      901 tgcaagcacg atcaacaaag tcatcgccgc tatcgacaac acggcgagca ccggtcgccc
      961 agggccttgt cgagcccgtc cgcccgaggt gctgcacgac ggcgtcaggt ccggcgtgga
     1021 tcaggccgac ctgccaatcc tcggcccacc ggaccagcta cggcgggcca ccggctcccc
     1081 ctcggacctc gacgatctcg ccatcccgat cttgctcccc cacgtggtga cccctgatga
     1141 cgagcctgtc cccaacgctg gccccccatc gctcctcctt tccgaaccgg ttcatgcccg
     1201 agctgcgcga ggcgtcaccg gaccacgagc accggtaccc gcgccgagtg caggaccctg
     1261 tgggcgacgc ttcccacaat cagccggctc cacaccgaaa gcccgcgcgt ccccatcacg
     1321 atgaggtcga cccgctcgtc ctcggccagg ccgaggatgc cgcgcgccct gcgaccgtac
     1381 gccgccgaca cgacttcccc acgggcgctt cgaccggcgt cgttcatacg tcgaaagtga
     1441 cgctctgcga ggtcgttggc gtcctcgagc tgctcgaggg atacgtcgac gccgtagacg
     1501 tactcccgct cacacacgtg caggacgatg acctccgcgc cacatcagga tgcgaggtcg
     1561 gccgcgatgt gagccgctcg atcactcgct ccgaaccgtc gatcggcaac aggatcttgt
     1621 cgaacatgtc gcgtcccccc caaacgttca gacgatcaac acagggacgt cgccgccgtg
     1681 tgcgagcttc gaggccacgc tcccgaggag aacgtggctc gcaccgcgac cgcgccgtcc
     1741 cacgacgatc aacgcatagc cctcctcgac ggcgaacttc cggagcgcct cggccgggcg
     1801 gccagagagc aggacggtct ccggtcgcaa cgcggtggcg ttcgcggtac gaatgaggtc
     1861 gcccctggcc cgttcgttgt cctcggccag gcccgacccg accgcgacgt cgtagtcgac
     1921 gacggccgcc agcgtcaggc gaccgaggcg cggtccaagc aggccgatcg ccgtccgaag
     1981 cgcgtcctcc gcttcggcag aaccgtcgat ccccacgagg acgtccacgc cgccggtcga
     2041 ggggcgagcg ggcgagagtc gcttgagtag acccggccgg tcgtggagaa gcgcgttcgc
     2101 cgccagaggc aggaccagcg gtccgaacgt gatcccgagc acgagccaca tgaagggctg
     2161 gtgccctcgc cgccccatca ccagggccat cgacaccccg agcaggctcc aggccaacgc
     2221 aacgatgatg aagatctccg atgaatccac gcctccaccg tagagacggg agcacgcaca
     2281 gtcgcggtcc atgtgacccg ttaccgacag gaccgtcgac cgcatcggat gagcgacaag
     2341 tcgaggcact cgaggcagcc gaaccgcccc aagtcgtgag tcgaaacagc tgagttccgc
     2401 agatgggggc tgctgccggc ccgtagacga gtctcgggat gatgggacga aggaattgcc
     2461 atcgaccgat gccgacagat ccgatggttg cgtggtcctc cgtggcagat ggagtccgag
     2521 ggctcgcagc ctcacatgat ccgaccgagc cgcgtccgag tgaccaggag ccatccgagc
     2581 gcgaacgagc ctgcgatgcc ggcggccgcc accagcaccg ccttgacctc gagcggcccg
     2641 ttgagcggac gcagggcgag tgcggccacg acaagcggga acccctggaa catgaaggcg
     2701 gcgtaggcgg cgcggcccat cgctcgagcg gttcggccgg cacgatccca gtgctgctgc
     2761 gcaaacgcga ggacccagac cgacgccgcg accgacatcg cgcccccgat cgtcgcgagc
     2821 acgagcgcct cccagcgcca tcccccgccg aatgcctcca cggatcgatc ggggtcctgc
     2881 gaggacgcga acgccacgat cagcgccacg gtcgccagca gcgccaccca gccgcatccc
     2941 cgccggatcc tttcggggac cggccggagc cacccgcgcc gcgcgctcac gatccccagg
     3001 ccgaacagcc cgatgcactc gggccattgc cagacgtgcg cgttggtgaa ctggagcgag
     3061 tcgaacggga accagcgtcg gacgacgaag gtggcgatca cgatcccggc ccccagcccc
     3121 accaggtggc gggcacgcag cggccccgcc ctgacggcgt tcggagtccg tccctggcgc
     3181 gttcgccatc cgacccaggc ggcgtagacc agcgagtaga tcagcaggac ctccacgaac
     3241 cacaacggtc cgttgtccag ggtcggctcg tccttgtaca cgtcccacag gctccagtcg
     3301 gatcccgcgg cgcgatacag gcctaacgtc aacagaggcc acagcacgaa catgaaggcg
     3361 gcgaggggaa tcccgagccg gatcagccgg tcgcgggcga aacggcccgg acccttgcgt
     3421 tcgagcgacg ccggcgtgag caggcccgcc accaggaaga acagggccat cacgaacacc
     3481 gcgaacggga ggatcag
//
LOCUS       DASUYD010000193         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1442057, whole genome shotgun sequence.
ACCESSION   DASUYD010000193 DASUYD010000000
VERSION     DASUYD010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1442057"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF195_10740"
     CDS             complement(<1..534)
                     /locus_tag="VF195_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HEX6581334.1"
                     /translation="MKRIIGLALALTATMALGANSPAAASEREKFSLNVVHGIPGVTV
                     DVCVNGAKAITDFQPGDVVSDVRLQEGEYRLKVTPAGEPCSAAILQARADLEGGRYRS
                     YTVVANLDDGGTPNLSLFRNPMRVTEAGSARLVIRHTADAPAVTVWADGSKLNPGRGF
                     EWGETRRYGVPAGTYSVA"
     gene            complement(721..1623)
                     /locus_tag="VF195_10745"
     CDS             complement(721..1623)
                     /locus_tag="VF195_10745"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX6581335.1"
                     /translation="MNDEASEGGDGLAIAAVARALKIPIPTIRSWERRYGLPAPPRTA
                     GKHRRYSRTEIDELRGLRDLIVRGYPVRTAAAMIRARRSGDDDGGAMEQMVRFAMRLD
                     SEGVRTELDRAAERVGVEATLRDVALPAMHEIGDRWKTGACGIEQEHLATDVVRRWCA
                     RRTAMAPPSSRPSPVVLACGPKDLHTIGLEAFAVVLAGRGWPCRVLGALTPTGSLVDA
                     ITALKAPAAVVVAQRGVTRRAAIESVAAVDALPRVRVFYAGDAFAAPTVRRGVPGVYL
                     GTDVVAAAEILESELTRLGLTSLA"
     gene            complement(1786..2667)
                     /locus_tag="VF195_10750"
     CDS             complement(1786..2667)
                     /locus_tag="VF195_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6581336.1"
                     /translation="MPAPPASGRRLAAFSLVLALAVSALPGEVMAADPVIVAAGDIAC
                     QDPCRAQRRTARLIGMIDPKAVLTLGDNQYEDGRFTDFMDSYRPTWGRFKRRTLPTPG
                     NHDYHVPGARGYFSYFGRRAHRRSGGMYSVNIGNWHVVSINTGNGVSDRKLRWLGRNL
                     SNDHHRCELAFWHHPRWSSGSIHGSNPQMGPLWRVLFRHGVDVALNGHDHLYERFALM
                     NPRRELAPLTGIRQFTVGTGGRGFHAAGPPIRGSQRVIDDRFGVVRMRLHPRGYSWRF
                     VAVGGSVLDLGSHACHG"
     gene            2839..2991
                     /locus_tag="VF195_10755"
     CDS             2839..2991
                     /locus_tag="VF195_10755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581337.1"
                     /translation="MNVEMLKIAIDAVAESHTEGHDCDVCKAARGDQDAFGRIALGAF
                     AEEEES"
BASE COUNT          521 a         1155 c         1157 g          522 t
ORIGIN      
        1 cgccaccgag tacgtcccgg cgggaacgcc gtagcgacgg gtctcgcccc actcgaaccc
       61 acggccgggg ttcagcttcg agccgtcggc ccacacggtc accgcgggcg cgtcggccgt
      121 gtgccggatc acgagacggg cggagccggc ctccgtcacg cgcatcgggt tccggaagag
      181 cgacaggttc ggcgtccccc cgtcgtcgag gttcgcgacc accgtgtagc tgcggtagcg
      241 ccccccttcg agatcggctc gcgcctgcag gatcgcagcg ctgcacggct cgcctgccgg
      301 ggtgaccttc aggcgatact cgccctcctg cagccgaacg tcgctcacga cgtcacccgg
      361 ctggaagtcg gtgatcgcct tcgcgccgtt cacgcacacg tcgacggtca ctcccgggat
      421 cccgtgcacg acgttcaacg agaacttctc gcgctccgac gcggcagcgg gcgagttggc
      481 gcccagggcc atcgtcgcgg tcagcgcgag ggccagaccg atgatccgct tcatggattt
      541 cctccctttc ctcgatcgtt ccgatgcctt cctagcggtc cgttttcccc ttcatcgatg
      601 gacatcacct ctctgtttcg gacgtggaga catcctgcag gatggaggca gctgaaacta
      661 tgcatgaacc tatgcaatcc acggccggat cacccccggg gcttgtcggc ggcgctcggg
      721 tcaggcgagc gaggtgaggc cgagccgcgt cagctcggac tcgaggatct cggctgcggc
      781 gacgacatcg gttccgaggt agacccccgg cacgccacgg cggaccgtcg gcgcggcgaa
      841 ggcatccccc gcgtagaaca cccgaacacg ggggagtgca tcgacggcgg cgaccgactc
      901 gatcgccgcg cggcgcgtga cgccgcgctg ggcgaccacc acggcggccg gcgccttcag
      961 ggcagtgatc gcatcgacga gggaaccggt cggggtcagc gcccccagga cgcggcacgg
     1021 ccatccgcgt cccgcgagga cgacggcgaa cgcctcgagc ccgatcgtat ggagatcctt
     1081 gggaccgcac gcgaggacga ccgggctggg acgcgagctc ggaggcgcca tcgccgttcg
     1141 acgggcgcac caccggcgga ccacgtcggt ggccaggtgc tcctgttcga tcccgcacgc
     1201 gcccgtcttc cagcgatcac cgatctcgtg catcgccggc agcgcgacgt ctcggagcgt
     1261 cgcttcgacg ccgacacgtt cggccgctcg gtcgagctcc gtccgcacgc cctcgctgtc
     1321 gaggcgcatc gcgaaccgga ccatctgctc catcgccccg ccgtcgtcat cgccggaccg
     1381 tcgggcgcgg atcatcgccg ccgccgtccg gaccgggtag ccgcgcacta tcaggtcacg
     1441 aagacctcgc agttcgtcga tctccgtgcg cgagtagcga cggtgcttcc cggccgtccg
     1501 aggcggagcg ggcagcccat agcgtcgctc ccacgaacgg atcgtgggga tcgggatctt
     1561 cagcgcgcgg gcgacggccg cgatcgcgag gccgtcaccg ccctcggatg cctcgtcgtt
     1621 catagccccg atgctacgcg cgccttcgac ctcgaaccta tgcatccgca cgtgccatga
     1681 acggtgggtc ctcgctgaag ctatgcacac cggagcccct cagaggctgg cgggctcggc
     1741 caactgggag atgttccgcc gcctctgcag agttacgccg gcggatcagc cgtggcaggc
     1801 gtgggagccc aggtcgagca cgctgcctcc tacggcgacg aaccgccagg agtatccgcg
     1861 cggatggagc ctcatgcgaa cgacaccgaa ccggtcgtcg atcacccgct gcgaccctcg
     1921 gatcggcggg ccggccgcgt ggaagccgcg tcccccagtt ccgacggtga actggcggat
     1981 cccggtgagc ggcgcgagct cacgtcgcgg gttcatcaac gcgaaccgct cgtagagatg
     2041 gtcatgaccg ttcaacgcga cgtccacccc gtgtcggaag aggacccgcc agagcggccc
     2101 catctgcggg ttggagccgt ggatcgaacc ggaagaccag cgggggtggt gccagaacgc
     2161 gagctcgcaa cggtggtggt cgttcgagag attccggccg agccaccgga gcttccgatc
     2221 cgagaccccg ttccctgtgt tgatcgacac gacgtgccag ttgccgatgt tcacgctgta
     2281 catgccgccc gagcggcgat gcgctcgccg accgaagtag ctgaagtatc cgcgcgcccc
     2341 cgggacgtgg tagtcgtggt tgcccggcgt cgggagggtc cggcgcttga accgccccca
     2401 cgtcggccgg tatgagtcca tgaagtcggt gaaccgaccg tcctcgtact ggttgtcgcc
     2461 gagggtcagg accgctttcg ggtcgatcat cccgatcaga cgcgctgtcc ggcgctgcgc
     2521 gcgacatgga tcctggcagg cgatgtcgcc tgcggccacg atcacgggat cggccgccat
     2581 cacctcgccc gggagcgccg acacagcgag cgcgaggacc aggctgaacg cggcgagccg
     2641 gcgtccggat gctggggggg cgggcatctc tcggcagcct aacgggcact gtgcgcgcat
     2701 ggcgtgccgg tcgtcggatg cccggcggtc cagatggtgt ccgtgtgtcc gcgacgaacc
     2761 gctgtcacag cgccgagggt tcctgacggt tgatggcgtt catggagctc cgctggtggg
     2821 tatccggacg aggaagagat gaacgtggag atgctcaaga tcgccatcga cgcggtggcc
     2881 gagtcgcaca ccgagggcca cgactgcgac gtgtgcaagg ccgcgcgtgg ggaccaggac
     2941 gccttcggaa ggatcgccct gggggccttc gccgaagagg aggagagttg aaggtccccg
     3001 tagtcgtagg aacaaggacc cccctgcgcc cggcccaccg ctccatcgtg ccgcgatgac
     3061 ctagcctccc gggtggtggt gatgttgcgc aggatgctcg aggacgatcg acgccgagcg
     3121 ggctgctcgt ggaagttctt ccgcgctcag ggagggacca gtacggtcgc tgactctggc
     3181 agtaccgcct gtcatgccta gaggcttgtc tcggagggtg ttcctgagcg ccttggcggt
     3241 cgggggcgcc tacctggcgc tgcgtcagtg gggcaggccg gaccggcgtc cttctgggcc
     3301 gagccagacg ccgccaaccc caagttcgtc gccggcctcg gatgaaggcc tgtac
//
LOCUS       DASUYD010000194         7405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1883810, whole genome shotgun sequence.
ACCESSION   DASUYD010000194 DASUYD010000000
VERSION     DASUYD010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1883810"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VF195_10760"
     CDS             complement(<1..254)
                     /locus_tag="VF195_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581338.1"
                     /translation="MRRLLLFVVLATCAAFTLPQIASAAVPKCFGEKATIVGTTKADH
                     IKGTAKADVIVGLGGSDVIKGLGRSDLICGGNGSDRLIGG"
     gene            complement(568..1701)
                     /locus_tag="VF195_10765"
     CDS             complement(568..1701)
                     /locus_tag="VF195_10765"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX6581339.1"
                     /translation="MIRTTVLGRRSFAGARSALVALLVAAFAIATGPAPASATAPAHA
                     ERRFTVMTYNVYLGANLQPLFAPVSDPLELIRRAKDIFDHLDEVDFNVRAVAVAEQII
                     ENDPDIVALQEVSLWRTALRSTPAEITVRYDFLKILLDELARQGHPYRTVSISENFSG
                     RLPISLTTDGLYTDRNAIIVPVDEAVSELITSNPMDGVFETSLPVSIGGNPLEVTRGW
                     ASTDVTIRGKTYRLFDTHFEAFDGRVRFGQVNELAQIMSESPHPAVLAGDINLYPMGV
                     RPEDATTWDVLTRSGFADAWVQADCFEPRYTAGQTDDLDNVPSILDNTVDYVLFDADD
                     DLDAVEGSCDIAGEELDDRTNTMPRLWPSDHAAVVVDLHIAKP"
     gene            1942..3192
                     /locus_tag="VF195_10770"
     CDS             1942..3192
                     /locus_tag="VF195_10770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581340.1"
                     /translation="MATSSDERPALPPLPGRRGLWLGILAYRWVAYVWMVLLAIGAAE
                     EFREPAVAWLVLCATGLWTLWLSVTPGWDRALTRWLDLGVCMGLLLVSGIVMREGYTV
                     GGHPFFATAYPVCAALTWGAARGVGPGLAAGFALSVALTLSRAINGVSLADATVDQIE
                     GLGNGIVYYIAAGGAMGLIARTLGRASEELRRSQEEVLRERERVARLSEHATMGRQIH
                     DSVLQALAWVNKRGRELGAEASVPGREVLRLADMAAQQERALRALIQYEPEEPPKERA
                     SLRDALDADARAVGEVQVTVTALGPIWLPEHEIDEVTAAVRQALENVVQHAAATHAVV
                     FAEEGEQGLVVSVRDDGVGFVYEEDGLRSRGKFGLLESMKGRIEDLGGRMIVRSAPGS
                     GTEVEFLIPEPGLGPDRAGDGGPP"
     gene            3189..3887
                     /locus_tag="VF195_10775"
     CDS             3189..3887
                     /locus_tag="VF195_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019327964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581341.1"
                     /translation="MSDEGPDGMTGDEPHPVEPIRVMVVDDHPVWRDGIRNDLESSGV
                     ATVVAEASDGGEAIDRARESMPEVILMDLQLPTVPGVEAIRAIVEESPHVKVVVLSAS
                     GEETDVLEAVKVGATGYLLKGSTGGEIVDAVRRVRDGEPVFTPSLAGMVLSEFRRVAA
                     PEPGVPGLTPRENEVLKLVAKGYTYREIAGKLFISVKTVQNHVQNILTKLQFRKRYEL
                     MRYAIQRGLDKAPE"
     gene            4019..5755
                     /locus_tag="VF195_10780"
     CDS             4019..5755
                     /locus_tag="VF195_10780"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HEX6581342.1"
                     /translation="MTTDLDRLIERFERLERDVEELKRTSPGERTVDAVTEPGTFAPP
                     VSDATPPMPAGVGSAGSVVLGEPIPDALDLEESVVGMWFPRLGALALLLGAGFGFRYA
                     VDRGWIGPGARVASGVALGVLLLLLGETTKRRGWAGYAQAVTGGGIAILYLTVWASFA
                     LYGMVPGAVAFALLVGVTTVAAGLALRHDSVALAVLAVLGGFLNPIVVGRAAIDAAGV
                     YAYTVALDLGVFALASVRRWGLLDKVAFVGSWTLYGLAQASGGRGMIFATTIFVLFGS
                     LPYLRSVVRGHLSQPADVIQMTTNAAVYYAVSYANLPPDQTGPFTLTLSCVFLAQGLA
                     VFAIAQGDRLLRLGTLGVSVGLFTLWIFLEGNPTWLPALWAAEGLGLTLIGSRVHSSP
                     TRLAGAVVVGFSVVATLYSFEDFIPERLFGSEEALVYVCVIAVLYAVAFVTSRIPAER
                     PYAAVAGVAANILTLVWLSLEVRAAVESARVLAFSVSAIWATYAALLLAVGIASRLRS
                     ARLLAIGVFSVTLIKMVGSDLWSIGQVHRIVSFAGIGVLLLACSLLFHRFKHVVLGEK
                     SGAVMTSSPTGR"
     gene            5968..6414
                     /locus_tag="VF195_10785"
     CDS             5968..6414
                     /locus_tag="VF195_10785"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581343.1"
                     /translation="MQFENTVTIRRPAEDVFVFLADFENLPSWNHAIQSTTKTSPGPV
                     GVGSTYRQIRSEPSRSEEGFEVTVFEPPRRLAIDGEIGPFHARVDYRLEPIEGATRLI
                     NGVELRPSSAVSKVLAPFARSRVKAGVAENLDTLKQILETGPRPSV"
     gene            6467..>7405
                     /locus_tag="VF195_10790"
     CDS             6467..>7405
                     /locus_tag="VF195_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581344.1"
                     /translation="MRSGTTSPPAGQPDELSATELAAKAGISPEEVERLVTCGILIRR
                     DGSQPFRAIDALKIRVARACEEGGLPMEGMAEAVRAGLLSFAFVESWPFERWSSRRPQ
                     THLELAEEVGLSFEALQRLVEAFGFARPEPKDVVVEAERPIAVLAGRIVELEIVDDAN
                     LARLGHVYAEAFRRIALAETEIYHSGVEEPLLRSGLGESRTMELASSLSPALTGMLDD
                     AVMAAYRRQQELTWTEHQIEHVEQALEDAGVSLPATPPTAMCFLDLSGYTRLTEERGD
                     QEAAALAGRLSDIVQQRSRLHGGEAVKWVGDGVMFRF"
BASE COUNT         1106 a         2439 c         2587 g         1273 t
ORIGIN      
        1 tctccgccga tcagcctgtc cgatccattc ccgccgcaga tcaggtcgct gcggccgagg
       61 cccttgatga cgtcgctgcc accgaggccg acgatcacat cggccttggc cgttcccttg
      121 atgtgatcgg cctttgtggt accgacgatc gtggcctttt ccccaaagca cttcggcacc
      181 gcggccgagg cgatctgcgg cagcgtgaac gcggcgcaag tcgccaggac cacgaaaaga
      241 agcagccttc gcatcttccc cacctccctt tggggggtgc gctcctcccc gtgggggagc
      301 gtgtgccctc cgatgcgaac cgagcctgcc ccatgctggc cgcttgctgg tagatgcgca
      361 agtccaactg gccgcttgga ccgttttcca cgaccgacct caaccccggg tagccgtcgc
      421 cccgctgcgt cggaagcggc aaccagggcc tcgcccgcga cccgtgccgg caagcgttcc
      481 cccaaccatg ctcccgcgcc gtggcctagt cgcatgtcat gccttacttc cctgacagcg
      541 tccatcttcg tgcctcctga cccagcctca gggcttcgcg atgtggagat ccacgacgac
      601 cgcggcgtgg tcagagggcc acaacctcgg catcgtgttc gtacgatcgt cgagctcttc
      661 gccagcgatg tcgcacgaac cctccaccgc atccaggtcg tcatccgcgt cgaacaagac
      721 gtagtcgacc gtgttgtcca agatcgacgg aacgttgtcg agatcgtccg tctgcccggc
      781 cgtgtagcgg ggttcgaagc aatctgcctg gacccacgcg tccgcgaaac cggatcgcgt
      841 gaggacatcc caggtcgtcg cgtcctcggg ccgcacgccc atcgggtaga ggttgatgtc
      901 gccggccagc acggcgggat gcggcgactc cgacatgatc tgggcgagct cgttcacctg
      961 cccgaaacgc acccggccgt cgaacgcctc gaagtgtgtg tcgaaaaggc gataggtctt
     1021 gcctcggatc gtcacgtccg tgctcgccca ccctcgcgtg acctcgagcg ggttcccgcc
     1081 gatcgagacg ggcaagctcg tttcgaagac gccgtccatc gggttcgacg tgatgagctc
     1141 ggagaccgcc tcgtcgaccg gcacgatgat cgcgttgcgg tcggtgtaca ggccgtcggt
     1201 tgtcaagctg atcggcagcc gtccgctgaa gttctcactg atggataccg ttcggtaggg
     1261 gtgcccctgc cgtgcgagct cgtcgaggag gatcttgagg aagtcgtacc tcaccgtgat
     1321 ctcggcgggg gtggatcgca gtgccgtgcg ccagagagag acctcctgca gggcaacgat
     1381 gtcgggatcg ttctcgatga tctgttcggc gaccgcgacc gcccggacgt tgaagtcgac
     1441 ctcgtccagg tggtcgaaga tgtccttcgc gcgccggatc agctccaggg gatccgatac
     1501 aggggcaaac aggggctgca ggttcgcgcc gaggtagacg ttgtaggtca tcacggtgaa
     1561 ccggcgttcg gcgtgtgccg gcgccgtcgc cgaagctgga gcaggccccg tcgcgatcgc
     1621 gaacgcagcg acgaggagag caacgagtgc cgagcgcgcc ccggcgaacg accggcgtcc
     1681 gagcactgtc gttcggatca tggtgtttac cctccttttc catggaccga catcgatcga
     1741 cttctgactc cgatcgaccc ttcacgatcg gcgggttcat gtttgacgtt tcgggacccc
     1801 gtcaccagac ggagaaccgc tcatcccggc gatgccaact acccagacct tccgggtgct
     1861 tcgggaaacc tcgaaccgcc aacgttggcc gttgaccacg ccggtggagg ctcgctaggc
     1921 tcggccggga ggtcggcgct ggtggccaca tcgtccgacg aacgcccggc gctcccgccg
     1981 ctgcccggcc ggcggggcct gtggctgggc atcctcgcgt atcggtgggt ggcctacgtg
     2041 tggatggtgc tcctggcgat cggcgccgcg gaggagttcc gcgagccggc ggtcgcgtgg
     2101 ctggtcctct gtgcgaccgg cctctggacg ctctggctct cggtgactcc cggatgggac
     2161 cgcgcgctga cccggtggct cgacctcggc gtgtgcatgg gtctcctcct cgtgtcgggc
     2221 atcgtcatgc gcgaggggta cacggtcggg ggccatccct tcttcgccac cgcctacccg
     2281 gtctgcgccg ccctcacctg gggcgcggcg cggggggtgg gcccggggct cgccgccggg
     2341 ttcgcgcttt ccgttgcgct cacgctcagc cgcgccatca acggcgtttc tctcgccgac
     2401 gcgaccgtcg accagatcga gggcctcgga aacgggatcg tgtactacat cgccgccggg
     2461 ggcgcgatgg ggctcatcgc tcggaccctg ggacgtgcct cggaggagct ccgtcggtca
     2521 caggaggagg tcctcaggga acgggaacgc gtggctcgac tgtccgagca cgccacgatg
     2581 ggacggcaga tccacgactc ggtccttcag gctctggcct gggtcaacaa acgggggcgc
     2641 gagctcgggg cggaggcctc ggtgccgggc cgggaggtcc tgcgcctggc cgacatggcg
     2701 gcgcagcagg agcgggcgct gcgagcgctc atccagtacg agcccgagga gcctccgaag
     2761 gaacgggcct ccctccgtga cgccctcgac gccgacgcca gagccgttgg ggaggtgcag
     2821 gtgacggtga ccgcgctcgg gccgatctgg ctccccgaac acgagatcga cgaggtgacg
     2881 gcggcggtcc gccaggcgct cgagaacgtg gtccagcatg cggcggccac ccatgccgtc
     2941 gtgttcgccg aggaggggga gcaggggctc gtcgtcagcg tgcgcgacga tggcgtcggg
     3001 ttcgtgtacg aggaggacgg tctccggagc cgggggaagt tcggcctctt ggagagcatg
     3061 aagggccgga tcgaggatct gggcggccgg atgatcgtgc gctccgcgcc cgggtcgggg
     3121 accgaggtgg agttcctgat ccccgagccc ggactggggc ctgatcgtgc tggcgatggg
     3181 ggaccaccat gagcgacgaa gggccagacg gcatgacggg agacgagcca caccccgtgg
     3241 aaccgatccg ggtcatggtg gtggatgacc atcccgtctg gcgcgatggg atccgcaacg
     3301 atctggaatc ctccggcgtc gccacggtgg tcgccgaggc ctcggatgga ggcgaggcga
     3361 tcgaccgagc ccgcgaatcg atgccggaag tcatcctgat ggaccttcag ctccccaccg
     3421 tcccgggcgt ggaggcgatc cgtgccatcg tggaagaatc accgcacgtc aaggtcgtcg
     3481 tgctctcggc ctccggtgag gaaaccgacg tcctcgaggc cgtgaaggtc ggcgcgacgg
     3541 gctatctgct caagggctcg accggaggcg agatcgtgga cgcggtccgg cgcgttcgcg
     3601 acggagagcc ggtgttcacc ccatcgctcg ccgggatggt tttgtccgag ttccgccggg
     3661 tcgcggcccc cgagccgggc gtcccgggcc tcaccccacg cgagaacgag gtgttaaagc
     3721 tggtggcgaa gggctacacc taccgagaga tcgccggcaa gctgttcatc tcggtcaaga
     3781 ccgtccagaa ccacgtccag aacatcctca cgaagctcca gtttcgcaag cgctacgagc
     3841 tgatgcgcta cgcgatccag cgaggcctcg acaaggctcc cgagtaatcg cctgccggaa
     3901 gtccccaccc ggagatcggg atccttcggc agccgaacca gggaagttcg cgtctggtct
     3961 gcttccgcca tccggcccaa gctgcccggg tgaaccgagc tgcgaacggg tgcggacgat
     4021 gaccacagac ctcgatcggc tgatcgaacg cttcgagcgt ctcgagcggg acgttgagga
     4081 gctgaagcgg acgtcgcccg gcgagcgaac ggtcgacgcc gtcaccgagc ccggcacgtt
     4141 cgctccgccg gtctcagacg ccacaccgcc gatgccggca ggggtggggt ccgcggggtc
     4201 cgtagtgttg ggcgaaccca tccccgacgc cctcgacctc gaggagagcg tcgtgggcat
     4261 gtggttcccc cggctggggg cgctggctct cctgctcggg gccgggttcg gcttccgata
     4321 tgcggtggat cgggggtgga tcggcccggg ggcacgcgtg gcgtcggggg tcgccctcgg
     4381 cgtgctcctg ctgctcctcg gggagacgac gaagcgccgc ggttgggccg gctacgcgca
     4441 ggccgtgacc ggcggtggta tcgccatcct ctatctgacg gtctgggcat cgttcgcgtt
     4501 gtacggcatg gtcccgggcg cggtcgcctt cgcgttgctc gtcggggtca cgaccgtggc
     4561 cgcgggtctc gcccttcgcc acgactccgt ggcgctcgcg gtgctcgcgg tgctcggtgg
     4621 tttcctcaac cccatcgtcg tggggcgagc ggcgatcgat gctgcgggcg tgtacgcgta
     4681 cacggtcgcg ctggacctcg gggtcttcgc actggcttcc gtgcggcgtt ggggactcct
     4741 ggacaaggtc gcgttcgtgg ggtcgtggac gctctatggg ctcgcgcagg cctccggcgg
     4801 tcgagggatg atcttcgcga cgacgatctt cgtgctcttc ggatcgctcc cgtaccttcg
     4861 gagcgtggtg cgcgggcatc tgagccagcc cgcggacgtg atccagatga cgacgaacgc
     4921 cgcggtctac tacgccgttt cgtacgcgaa cctcccgccg gatcagaccg gtccattcac
     4981 gctgacgctc tcatgcgtct tcctcgcaca aggcctggcg gtgttcgcga tcgcacaggg
     5041 tgaccggctg ctccgtctcg gcacgctggg agtctcggtg gggctgttca cgctgtggat
     5101 ctttctcgag ggcaacccga cgtggctgcc tgcgctgtgg gcggcggaag gcctggggct
     5161 cacgttgatc gggtcccgcg tccactccag cccgacccgg ctggccggcg cggtggtcgt
     5221 cgggttttcg gtcgtcgcaa cgctctactc gttcgaagac ttcatccccg agcgcttgtt
     5281 cggatcggag gaggcgctcg tctacgtgtg cgtgatcgcg gtcttgtatg ccgtcgcctt
     5341 cgtgacgtcc cgaattccgg ccgaacgccc gtacgccgct gtcgcgggcg tcgcggcgaa
     5401 tatcctcacc ctcgtatggc tgagcctcga ggttcgagcg gcggtggagt cggcccgcgt
     5461 gcttgcgttc agcgtctcag cgatctgggc aacgtacgct gccctgcttc tcgcggtcgg
     5521 aatcgcatcc cgtcttcgct cagcgcggct gctcgccatc ggcgtcttca gcgtcactct
     5581 gatcaagatg gttggaagcg acctgtggtc gatcggtcag gtccaccgga tcgtgtcgtt
     5641 cgctgggatc ggcgtcctcc tgctcgcatg ctcgctgttg tttcatcggt tcaagcacgt
     5701 ggtgctgggt gagaaaagcg gtgccgtgat gactagttcg cccaccggga ggtgacatga
     5761 ggacatcgga aagggcctca taggaaggct gcatgtgtgc gtcgctccgg ctccggaact
     5821 cgcgcgggcg gccgcgctct acgttgaaga acgggggccg gctgggatcg tgtcatccga
     5881 gggggggcca gcttcgtcac ccacagccgg ccgatatcga aacggaatca cgaggcgtca
     5941 ggagatcccc ggacgaaggg gacgtacatg cagttcgaga acaccgtgac gatccgaagg
     6001 ccggccgagg acgtcttcgt gtttcttgcg gacttcgaga acctgccgag ctggaaccat
     6061 gcgatccagt cgaccaccaa gacctctccc gggccggtcg gggtagggag cacctatcgc
     6121 cagatccgct ccgagccgag caggagcgag gaggggttcg aggtgaccgt gttcgagccg
     6181 ccgcgccggc tggccatcga cggcgagatc gggcccttcc acgcccgggt cgattaccga
     6241 ctcgagccga tcgagggagc gacgaggctc atcaacggcg tcgagcttcg gccgtcgtcg
     6301 gccgtctcga aggtcctcgc gccgttcgca cgttccaggg tcaaggccgg cgtcgccgag
     6361 aacctcgaca cgttgaagca gatcctggaa acgggtccgc ggccgtccgt ctgatctgcg
     6421 tggcccgggg cgccgggccg ctctcgccac gacgtacgat caccgcgtgc gaagcggcac
     6481 cacctcaccg ccggccgggc agcccgatga gctctcagcc acggagctcg cggccaaggc
     6541 cggcatctcg ccggaggagg tcgaacgcct cgtcacgtgc ggcatcctga tccggcggga
     6601 cgggagccag cccttccgag cgatcgacgc cctgaagatc cgggtcgcgc gggcgtgcga
     6661 ggagggtggc ctgccgatgg aaggcatggc cgaagcggtc cgggcggggc tcctgtcgtt
     6721 cgcgttcgtc gagagttggc cgttcgagcg atggagctcc cgcaggccgc agacccatct
     6781 cgagctcgcc gaggaggtcg gcctgtcgtt cgaggccctc cagcggctcg tggaggcctt
     6841 cgggttcgcc cgacccgagc cgaaggacgt ggtcgtcgag gccgagcggc cgatcgccgt
     6901 gctcgcgggg cggatcgtcg agctcgagat cgtcgacgac gcgaacctgg cgcgactggg
     6961 gcacgtctac gcggaggcct tccgacggat cgccctggcc gagaccgaga tctaccactc
     7021 cggagtcgag gagccattgc tccgctcggg cctgggcgag agcaggacca tggagctcgc
     7081 gtcgagcctc agcccggcgc tcacggggat gctcgatgac gccgtcatgg ccgcgtaccg
     7141 ccggcaacag gagctcacgt ggacggagca ccagatcgaa cacgtcgagc aggcgctcga
     7201 ggacgcgggc gtctcgctgc cggcgacgcc gccgacggcg atgtgcttcc tcgacctctc
     7261 gggctacacc cgcctgaccg aggagcgcgg tgaccaggag gcggcggcac tcgcgggcag
     7321 gctctccgac atcgtccagc agcgctcgcg cctgcacgga ggcgaagcgg tgaagtgggt
     7381 cggcgacggc gtcatgttcc gcttc
//
LOCUS       DASUYD010000195         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1223932, whole genome shotgun sequence.
ACCESSION   DASUYD010000195 DASUYD010000000
VERSION     DASUYD010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223932"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF195_10795"
     CDS             complement(<1..372)
                     /locus_tag="VF195_10795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013285921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6581345.1"
                     /translation="MSFDVAADAYDRFMGRYSRVLSPAFADYAGVRTGQRVLDVGCGP
                     GALTTELVHRLGPESVAAVDPSEPFVAAARARHPGVDVRRSSAEDIPHPDGAFDVAMA
                     QLVVHFMRDPIAGLTEMARVTR"
     gene            complement(575..895)
                     /locus_tag="VF195_10800"
     CDS             complement(575..895)
                     /locus_tag="VF195_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581346.1"
                     /translation="MGSSPGGGAGVVAGGDVGAGEDVGAGDGVGSGLGDGEGSDDGEG
                     SGVGEGVGEGFGGFEGFGGFEGSAATCVAMKVSSTEGSVFAAIADDTALRVRAIVRRA
                     TALA"
     gene            945..1277
                     /locus_tag="VF195_10805"
     CDS             945..1277
                     /locus_tag="VF195_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581347.1"
                     /translation="MTPSSYEAALDLFGRRADREDATSAPDGTGSLFATVGSIIDELT
                     SSDDPPIRAAEASSACPGSDCDSPGDVTQTRALVIVLAGLAIAFAGVLVIRARSRRST
                     TEVPSDRP"
     gene            complement(1303..1683)
                     /locus_tag="VF195_10810"
     CDS             complement(1303..1683)
                     /locus_tag="VF195_10810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581348.1"
                     /translation="MSRVLVVDDESIIGVILRHALDAKGHETLVAADGRTGIEIARAE
                     HPDAIVLDLMMPGVNGYDVLDAVRHSSELDEVPVLVLTAVTLSRERERCLAEGADAVI
                     TKPFDPGDVADAVEGLLSTQTKAS"
     gene            complement(2103..2240)
                     /locus_tag="VF195_10815"
     CDS             complement(2103..2240)
                     /locus_tag="VF195_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581349.1"
                     /translation="MGLFDPNRWPEPYPMFTSWKRVAIGALVLVVVVVGLFIVLPLIA
                     D"
     gene            complement(2297..2728)
                     /locus_tag="VF195_10820"
     CDS             complement(2297..2728)
                     /locus_tag="VF195_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX6581350.1"
                     /translation="MAEPSLYERLGGVFAIAAVVDHFSDAVVQNRIVGQGSKNPELRE
                     WHTNKLDRLPGLKFMRTLWLCNVAGGPFTFAATKPGSTPLGLEEAHRDLRISPEEFDE
                     VAAELGRSLDHFGVPPREKDEVLAAFAAHKGEVTEGSMASA"
     gene            complement(2821..>3102)
                     /locus_tag="VF195_10825"
     CDS             complement(2821..>3102)
                     /locus_tag="VF195_10825"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEX6581351.1"
                     /translation="VAGESELDLELDDFYFEPTVLTGSAGETLTLHLENEGDTEHNFS
                     LTDQGIDQDVEAGEKAEVSVTFPDSGTLVFFCKYHQDMGMRGALRVSSA"
BASE COUNT          526 a         1082 c          990 g          504 t
ORIGIN      
        1 ccgcgtcaca cgcgccatct ccgtgagtcc ggcgatcggg tcgcgcatga aatgcaccac
       61 gagctgcgcc atcgccacgt cgaatgcgcc gtccggatgg gggatgtcct cggccgagga
      121 tcgccggacg tcgacgccgg gatgccgtgc tctcgcggcc gcgacgaacg gctcggaagg
      181 atcgaccgcg gccacggact cgggcccgag ccggtgcacg agctcggtcg tcagagcgcc
      241 cggcccgcag ccgacatcga gcacccgctg tcccgtgcgg acgccggcgt agtcggcgaa
      301 agccggtgag agcaccctcg agtagcgccc catgaagcgg tcgtaggcat cggcggcgac
      361 gtcgaagctc atcctcgccg accatatcgt cggtcttctc gaggagggac gttgtgccgc
      421 cgcccacacc gagaacgcca aatactcccg gacggaggtt cctccatcac ttccaccggg
      481 taccgtgaaa cagggacctg cgaaggtcgg tcggcggcag gaaggcatct cacgtggcgg
      541 gaagctccag ggggcggcgt aggagacgag tccttcaggc gagggccgtc gccctcctga
      601 cgatcgctct caccctgagc gccgtgtcat cggcgatcgc cgcgaatacc gaaccctccg
      661 ttgacgagac cttcatggcg acgcaggtcg cggccgaccc ttcgaagcct ccgaagcctt
      721 cgaagcctcc gaagccttcg cccaccccct cgccgactcc cgagccctca ccgtcatcgg
      781 agccctctcc gtcgccgagc cctgacccaa cgccatcgcc tgcacccacg tcctcgcccg
      841 cacccacgtc gccgccggcg acaactcccg cgccgccccc cggcgatgac cccaccgctt
      901 caggaggagg gacgagggaa ggcaacacac ctgtcggctc gggcgtgacc ccgagctcgt
      961 acgaagcggc tctggacctg ttcggaaggc gtgcggaccg ggaagatgcg acctccgcac
     1021 cggatggcac cgggtcgctg ttcgcgacgg tcgggtcgat catcgacgag ctcacatcgt
     1081 cagacgatcc accgatccgg gcggccgaag cctcgtccgc atgccccggg tccgactgtg
     1141 actcccccgg cgatgtcacg cagacccgag cgttggtgat cgtcctggcc ggtctggcga
     1201 tcgccttcgc cggagtgctc gtcatccggg cgaggagtcg gcgatcgacc accgaagtgc
     1261 cctccgaccg accgtgagct tccggagaaa ctgacgctcg ggctatgacg ctttggtctg
     1321 tgtcgacagc agcccctcga ccgcgtctgc gacgtcccca gggtcgaagg gtttcgtgat
     1381 cacggcatca gccccttcgg cgaggcagcg ttcccgttct ctcgagagcg tgactgcggt
     1441 caggacgagg acggggactt cgtccagctc ggaggaatgg cgcaccgcgt cgagcacgtc
     1501 gtagccgttc acgcccggca tcatcaagtc gaggacgatc gcgtccgggt gctcggcccg
     1561 cgcgatctcg atccccgtcc gtccatccgc ggccacgagc gtctcatgtc ccttcgcgtc
     1621 gagggcatgg cgaaggatca caccgatgat cgactcgtca tcgactacga ggacgcggga
     1681 catccgaccc ccttcagtct cttgctctgt atcggcgctc aggcgccgga acctgaagcc
     1741 cagatgcatg cccatcagca cgtgaacgac ctggacagcc tcaccggctg acaggcggca
     1801 ggtgagggac tatcagcagc gccagctgaa gatccagcct cggggttcgg acgtgcgacc
     1861 ggggccggct cgccgctcac cgtaggagct gatctcgggc acggctcgga ggccttgggt
     1921 cagagcgagt tcctcggcca gtgcctgaca ctcctcgagc gttgatcgcc gatgggtgcg
     1981 cggaacggtg accacgtagg ttccgggacg ccgtgcgacg gagtattcgt ccacccgatc
     2041 gcagcgtaga ccgccggtag cgtcagggga aatcgagatg tgtgccgagg gttcacggtc
     2101 tgctagtcgg cgatcaacgg caggacgatg aagaggccga ccaccacgac gacgaggacc
     2161 agagcgccga tcgctacccg cttccatgac gtgaacatcg ggtagggctc gggccaccgg
     2221 ttcgggtcga acagccccat cgtgttccga ggatacggtg gcggatgacg ctgcgatgcg
     2281 gatgaaggcg cggccctcag gccgaggcca tcgatccctc ggtcacttcc cccttgtgcg
     2341 ccgcgaaggc cgccaggacc tcgtccttct cacgcggagg gaccccgaag tgatcgagcg
     2401 accgccccag ctcagcggcg acctcatcga actcctcggg ggagatgcgg aggtcgcgat
     2461 gggcctcttc gagaccgaga ggggtgctcc ctggcttcgt ggcagcgaac gtgaacggtc
     2521 caccggcgac gttgcagagc cacagcgtgc gcatgaattt cagtcctggc agccggtcca
     2581 gcttgttggt atgccactca cgaagctcgg ggttcttcga cccctgtccg acgatgcggt
     2641 tctggacgac cgcatcgctg aagtggtcga ccaccgcggc gatggcgaac acgccaccca
     2701 atcgctcgta caggctcggc tcagccatca tcgacctccc atctgcgacc gaaagggtgc
     2761 cccgccggcc gcgctctgtc tcacgtcctc attccacgcg tggtctggtt caaaacaggg
     2821 ctaggcgctc gagacccgaa gcgcccctcg catccccatg tcctggtggt acttgcagaa
     2881 gaagaccagc gtgccggagt cggggaacgt cacgctcacc tcggccttct ctccggcctc
     2941 cacatcctgg tcgatcccct gatcggtgag gctgaagttg tgctcggtgt cgccctcgtt
     3001 ctccaggtgc agcgtgaggg tttctccggc gcttccggtc agcaccgtcg gctcgaagta
     3061 gaagtcgtcc agctccagat cgagctcgct ctctcccgcc ac
//
LOCUS       DASUYD010000196         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1007866, whole genome shotgun sequence.
ACCESSION   DASUYD010000196 DASUYD010000000
VERSION     DASUYD010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1007866"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VF195_10830"
     CDS             complement(<1..623)
                     /locus_tag="VF195_10830"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX6581352.1"
                     /translation="MVTQDQVREALRSVNDPEIGRPIEDIGMLRDIEVLDGGLVRVHV
                     LITIEGCPLKDRITSDVTEALAPLEGVERVDVALSPMSDEQRASLVANLRGDAAPQQQ
                     PTFFTGSDTTVIAVASGKGGVGKSSVTVNLACALAAEGHRVGVLDADVWGFSVPRMMG
                     VTGKPVGFNDMILPLESHGVKVISMGFFVPEETPVIWRGPMLHKAIE"
     gene            800..1480
                     /locus_tag="VF195_10835"
     CDS             800..1480
                     /locus_tag="VF195_10835"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6581353.1"
                     /translation="MREPTPLEIHKALADDTRFRLYRYLGLSGRPVSVRELSTRLSLH
                     PNTLRPHLRRLEEAGLVRREVRKGSTVGRPQTMYIAIEQVERENRDFRLLAEILAGLA
                     SGARARDRASELAREWGRYLAARGVPKPGTRAGARHNLAVLQEAMASAGFDPRFRRAG
                     DGVVEVTLRDCPFRELLDDHRQLVCSIHQGLVEGMLGALEPPLSLDEFTPLTERATCR
                     LEVSEPRA"
     gene            complement(1495..2052)
                     /locus_tag="VF195_10840"
     CDS             complement(1495..2052)
                     /locus_tag="VF195_10840"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6581354.1"
                     /translation="MARRTVSWMVAAFLAVLIVPVAPAIAGGGCHQGVTQGNGDTVEM
                     VDACFTPTILTVDPGETVTFVNADPMTHNVTANVWGYFDDLNEGDAFTATFDQPGIYP
                     YACAYHPGMTGAIVVGSGTGAGNGETVSIDSVDLPQASPVVEVRTVTQDSGSAPIAIG
                     WIVGAALGLAIGLGIGFVLNKRAGA"
     gene            complement(2112..>2498)
                     /locus_tag="VF195_10845"
     CDS             complement(2112..>2498)
                     /locus_tag="VF195_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581355.1"
                     /translation="PSGPPPDEVYLVDSPFGDPGLIYAWRPSASNPTLPGTDWGLVLI
                     AFQGDDETVVKTVQRFEDVHDATVNGERASWIPVPHVLEIETDAGFETFSVQGNVLIW
                     EDDDVTYRLETSLDRASAIALAESTT"
BASE COUNT          358 a          899 c          838 g          403 t
ORIGIN      
        1 tgctcgatcg ccttgtgcaa catggggcca cgccagatca cgggggtttc ctccggcacg
       61 aagaagccca tcgagatgac cttcacgccg tgggactcga gcggcaggat catgtcgttg
      121 aacccgaccg gcttgccggt cacgcccatc atgcgtggga cgctgaaccc ccatacgtcg
      181 gcgtcaagta cgccgacgcg gtgaccctcg gccgcgaggg cacacgccag gttcaccgtg
      241 acgctggact tgcccacccc gcccttgccg gaggcgaccg cgatcaccgt cgtgtcggag
      301 cccgtgaaga aggttggctg ctgctgggga gccgcgtcgc cccgaaggtt cgcgacgagc
      361 gaggctcgct gctcatcgct catcggggac agggccacgt ccacgcgctc gacgccctcg
      421 agcggcgcga gggcctccgt cacgtcgctc gtgatccggt ccttgagcgg acatccctcg
      481 atcgtgatga gcacgtgcac gcggaccagg ccgccgtcca ggacctcgat gtcgcggagc
      541 atgccgatgt cctcgatcgg ccggccgatc tcggggtcgt tgaccgaccg cagcgcctct
      601 cggacctggt cctgggtgac caacggtgac ctcctcggat ccctggctcg gactcctgcc
      661 ccgacttgcc cgactctcct gggggctcgg attatcctga ttcccacggt gcgaaccgtg
      721 ctttccccca tggtacgtcc ggtgctcgcg ggcgtcgacg ctcgctccac ctgccgaaac
      781 cgaggagctt tcgttgccgg tgcgtgaacc gactccgctc gagatccaca aggcgctggc
      841 cgacgacacc cggttccggc tctaccgcta cctcggcctg tcggggcgcc ccgtctcggt
      901 ccgcgagctc tcgacgcgcc tgtccctgca tccgaacacg ctccggccgc acctgcgccg
      961 gctggaggag gccggcctgg tgcgtcgcga ggtccgcaag ggctcaacgg tgggccggcc
     1021 gcagacgatg tacatcgcga tcgagcaggt cgagcgtgag aaccgcgact tccgcctcct
     1081 cgccgagatc ctggccgggc tcgcatccgg ggcccgggct cgcgaccgtg cctcggagct
     1141 cgcccgcgag tgggggaggt acctcgcggc gcgcggcgtg cccaaacccg ggacccgcgc
     1201 cggcgcccgc cacaacctgg ccgtcctgca ggaggcgatg gcgtcagcgg ggttcgatcc
     1261 tcgcttccgg cgagccggcg acggtgtcgt ggaggtgacc ctgcgcgact gcccgttccg
     1321 cgagctgctc gacgaccacc gccagctcgt gtgctcgatc caccagggcc tggtcgaagg
     1381 gatgctcggg gcgctcgagc cgcccctgtc gctcgacgag ttcacgccgc tgaccgagcg
     1441 ggccacctgc cgtctcgagg tgagcgagcc tcgcgcctga tgcctccggc tggctcaggc
     1501 ccccgctcgc ttgttgagca cgaacccgat gcccaacccg atcgcgaggc cgagtgccgc
     1561 gcccacgatc cacccgatcg cgatcggagc gctgcccgag tcctgcgtca ccgtcctgac
     1621 ctccaccacg ggtgaggcct gtggcaggtc gaccgagtcg atggacaccg tctccccgtt
     1681 cccggccccg gtgccgctcc cgacgacgat cgctccggtc atccccgggt ggtaggcgca
     1741 cgcatatggg tagatacccg gctggtcgaa cgtcgcggtg aaggcgtcgc cctcgttcag
     1801 gtcatcgaag tacccccaca cgttcgccgt cacgttgtgc gtcatcggat cggcattgac
     1861 gaacgtgacc gtctcgccgg ggtcgaccgt gaggatcgtg ggggtgaagc aggcgtcgac
     1921 catctcgacg gtgtccccgt tgccttgggt cacgccctgg tgacagccgc cacccgcgat
     1981 cgcgggggcc acgggcacga tcaggaccgc gaggaacgcg gccaccatcc aggagacggt
     2041 gcggcgagcc atggcgactc ctttcagctc ggtctcggaa cctctacacc gcggagcggg
     2101 gctcggttcc atcacgtcgt cgactcggcc agggcgatcg ccgaggcccg gtcgagcgag
     2161 gtttcgagcc ggtacgtgac gtcgtcgtct tcccagatca agacgttgcc ctggacggag
     2221 aacgtctcga agccggcgtc ggtctcgatc tcgagcacgt gtggcacggg gatccacgag
     2281 gcccgctcac cgttcaccgt ggcgtcgtgg acgtcctcga accgctgcac ggtcttgacc
     2341 accgtctcgt cgtccccctg gaacgcgatc agcacgagcc cccagtcggt tccgggcagg
     2401 gtggggttgg aggcgctcgg gcgccacgcg tagatcagcc cggggtcgcc gaagggactg
     2461 tcgaccaggt agacctcgtc gggcggcggc cccgaggg
//
LOCUS       DASUYD010000197        10196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1230031, whole genome shotgun sequence.
ACCESSION   DASUYD010000197 DASUYD010000000
VERSION     DASUYD010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230031"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF195_10850"
     CDS             <1..252
                     /locus_tag="VF195_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581356.1"
                     /translation="LKLIGESLRESFSVQLDVPMDDRWIIGRHDGPEFVTRHSQCVQD
                     LDRARFLIGIDVLRPHPAVPIRPVTCLSSGALQVGSTVG"
     gene            complement(309..1652)
                     /locus_tag="VF195_10855"
     CDS             complement(309..1652)
                     /locus_tag="VF195_10855"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX6581357.1"
                     /translation="MIGAGSSGLAVLRALRGRGVTVRCFERGSQAGGLWRYQNDNGVS
                     SAYASLRTNVSRPRMEYPSFPMPTVYGDFPHHSDMAAYLEAYGESFGHRRFIRFRSTV
                     ERLEPVPDGTWRVLLDDGSVHRFRAAVVATGLHWCPKRPEHPGRFFGAVSHSHDYRVP
                     EPFARSRVLVVGGGQSAAEIAVEVSLVATRTYLSVRRGYHVLPRRIGGSPYDASDIPP
                     FNRMPWWLMNLLYARDVAREVGPIPREWPTPPRRLLEGIPIISTDLLPAIRRGDVLLK
                     PAVERLAGDRVRFADGSEEPFDRIIYATGYRISLPFVCPSLVAPRGREFPLYRRIVPL
                     GLRGLLFAGFVDVPGGLLPVVEDQAEWIAALLTGHLRIPPTKQMRRAIDRAERRTRER
                     FPEESAHSVRCDPHAYRRLLRSDLRRARRRALQREGPPLFDDLVRTLELSRKRMP"
     gene            complement(2721..2963)
                     /locus_tag="VF195_10860"
     CDS             complement(2721..2963)
                     /locus_tag="VF195_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581358.1"
                     /translation="MSGELWPARYEIRVEGVLEERWSDWFGGLSVFTEAGQTILFGTL
                     ADQSALHGVLDKVRDMGLSVIGMRRLPPEEHGGRPR"
     gene            3465..6269
                     /locus_tag="VF195_10865"
     CDS             3465..6269
                     /locus_tag="VF195_10865"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6581359.1"
                     /translation="MVGTRGSRDHPTHEREELLATKLATPHVRPGLLARPRLLKSLDE
                     AIDRGLTLVCTPAGFGKTTLLADWADRVKRPVAWLSLDPDDSDPVRFWRYVAAALERS
                     GVRVGERVRSLLEAPRGVSSEGVVTALLNELEASPDEVTLVFDDYHVIESRPVHDGMT
                     FLVSHLPPQLHVLMATRSDPPLPLPRLRARGQIAELRAADLRFTPEESAAFLREVWGL
                     DLSPEAVVVLESRTEGWVVGLQLAALSLRDRPDPDAFVDAFTGTHRYVLDYLSGEVLE
                     RQPERVRTFLLRTSILERLSGALCDAVTGGSDGQAMLEDLERANLFLVPLDEERRWYR
                     FHHLFRELLRARLQQTEISVVPDLHRRAAAWCERHGLIDEAVHHGLRSDDAPWAARLV
                     EQHLAETLGRAEEAHVERWLAQLPDDAVLSRPALCLAQGLRQLFLGRLDSVERFLGQA
                     ERVFHHGQESPAFGVLTEVGTVGEVPAAIALLRSELAGARGDPEGMAEYAASALSEMA
                     EEEHGPRFWARWLSGAEAELMRGRLAEAERASAELLAEGHAAPDPLPGMVSPLSWLGQ
                     VQQARGSLTAALRTHREALRLATLSSQARMKGPAPTTYIFTSAYQEGLAHLGIAQVLY
                     ERDLLEDALRHVEEGIELGRQETWFREHALVISAWIRDAMGQAETALEAMNEASRLQA
                     SPEVNSLWHPAASERARLLVAQGQTAEAERWTEERGLTEDDDVSYLRERDHLVLARVL
                     LARSEPGRALRLVERVDELARSQDRIRSLIQIRAVGSLAMQATGDHQGALALLADVLA
                     LGRPEGFIRVFADEGPPMAALLQSLIRARRHGGRAAASRAEREHVNRVVRAFRSPVGH
                     LEVPGTAPVEAGPIEPLTRRELEVLGLMAAGRRNQQIADELVVTLDTVKRHVSHIFDK
                     LGTASRTEAIARARELRFLP"
     gene            complement(6455..7387)
                     /locus_tag="VF195_10870"
     CDS             complement(6455..7387)
                     /locus_tag="VF195_10870"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX6581360.1"
                     /translation="MKILMLGAGVIGSVYSARLLHVGHDITVLARGERLIELTEHGLT
                     VENAETGERDELAVRAIGATDPGERYDLVWIVVQAPQLDSVLPLVQVISGEHDVLFFG
                     NTAGRTRELADALGDRCLFGFPGVGGVLDGHVVRYVLIRQQKTTLGEPTGRPSERMRS
                     LEALLIQAGFPTAISRDIEGWLLGHAAFVVPIAFALQRVDADPARLAADRGLLRQMVR
                     ATRQAFGALTATGAAEIPGNLRMLYRLPTAFAAGYWRRVLASPNGELWFGHTSAAPQE
                     MDDMASALKRAVRNTGRSTPDLDALLEPLRRSGM"
     gene            complement(7546..7680)
                     /locus_tag="VF195_10875"
     CDS             complement(7546..7680)
                     /locus_tag="VF195_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581361.1"
                     /translation="MPAYPRFPVTKGLTGVAVAAQHTIVSDDVSRDPRYLTAFETTGS
                     "
     gene            complement(7920..8741)
                     /locus_tag="VF195_10880"
     CDS             complement(7920..8741)
                     /locus_tag="VF195_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581362.1"
                     /translation="MATIASTMDRATGRRTTGLVTSTLVVAKRGLMKFVRTPQLVVVG
                     TIQGAMFLLIFRYVFGGAIEIPGISYVDFLVPGFITTGILFAVMDAAPGVAGDLEQGF
                     VDRLRSLPIPRSSFLSGRAIADTAVLVYSLAFMTLTGFAVGFRIHGSVPDALLAFALC
                     VAFGFAFIWVFITIGLFAGSAEAAQGMALLVFPLSFVSSAYVPVESMPGWMQAFAEHQ
                     PLTYMVDAVRALTLGPEAEALLGHPASYFVTRALLWAAGIVAVFAPLAVARYRRS"
     gene            complement(8762..9739)
                     /locus_tag="VF195_10885"
     CDS             complement(8762..9739)
                     /locus_tag="VF195_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6581363.1"
                     /translation="MTPTIEAHGLRKRFGRTVALDGLDVVAEPGAVLAVLGPNGAGKT
                     TFVRAVATLLRPDDGSLRVAGYDVRREPEAVRRLIGLAGQFAAVEPAMTGRENLEMVA
                     RLLGQPRRAARANAAAVLEELGLQEEAGRLVRTYSGGMRRKLDLGASLVGAPRLLLLD
                     EPTTGLDPRSRIELWDSIRRLVERGTDVLLTTQYLDEADHLASRIVIIDRGRAVASGT
                     PAELKRRIGGSVIEVHARRAEDLVAIAEAFGRLDHGEPQVDESTRRVSVTVDPGGDPL
                     RAGLRAIHDLGVEVDDVALRQPKLDEVFLALTGQPLHEETGRQSRTDAA"
     gene            complement(9744..9908)
                     /locus_tag="VF195_10890"
     CDS             complement(9744..9908)
                     /locus_tag="VF195_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581364.1"
                     /translation="MRRSTSPAEPSDAAGGGLHLETLDAGLADDRYAIEISGGAAHLW
                     ITVDDPTKEG"
BASE COUNT         1637 a         3568 c         3452 g         1539 t
ORIGIN      
        1 ctgaagctga tcggcgagtc gctccgcgag tcgttctcgg tgcagctcga tgtgccgatg
       61 gatgatcgat ggatcatcgg ccgccacgac ggcccggagt tcgtcacgcg gcattcccag
      121 tgcgtccagg atctggatcg cgctcgcttc ctgataggta tcgacgtcct caggccgcat
      181 ccggccgtgc cgatccgtcc ggtcacttgc ttgtcctccg gagcgctcca ggtcggttcc
      241 acggtggggt agtcgtcgtg gatcgggcat gtgggcgatc gtggatccgg ccggagcccg
      301 ggccaccatc acggcatccg cttccgggac agttcgagcg tccgtacgag gtcgtcgaag
      361 agaggaggcc cttcccgctg gagcgctcgc cggcgcgcgc gacggaggtc tgagcgcagc
      421 aggcgccgat atgcatgcgg atcacagcgc acgctgtggg cgctctcttc tgggaagcgc
      481 tcgcgcgtcc gccgttcggc tcgatcgatc gcccggcgca tctgcttcgt cggagggata
      541 cgaagatgcc cggtgaggag ggcggcgatc cactcggctt ggtcctccac caccggcagc
      601 agcccgcccg ggacatcgac gaagcccgcg aagagcagac cacggagccc gagcggaacg
      661 atccgccggt agagcggaaa ttctcgtccc cggggcgcta ccaacgaagg acacacgaag
      721 ggaagactga tccggtaccc ggtggcgtag atgatccggt cgaagggctc ctcgcttccg
      781 tcggcgaaac gcacgcggtc gccggccaag cgttcgaccg ccggcttgag caggacgtct
      841 cctctacgga tcgccgggag caggtccgtc gagatgatcg ggatgccctc cagcaggcgc
      901 cgcggcggcg tgggccactc gcgggggatc ggtccgacct cgcgcgccac gtccctcgcg
      961 tacagcaggt tcatcagcca ccacggcatc cggttgaagg gagggatgtc cgaagcgtca
     1021 taggggctcc cgccgatccg acgcgggagc acgtggtacc cgcggcgcac ggagaggtag
     1081 gtgcgcgtcg cgacgagtga gacctcgacg gcgatctcgg ccgcggactg gccaccgccg
     1141 accacgagga cccggctcct cgcgaacggc tcgggcacgc ggtagtcgtg cgagtggctc
     1201 accgctccga agaacctgcc cgggtgctcg gggcgcttcg ggcaccagtg gagcccggtc
     1261 gccacgacgg cagcacggaa ccggtgcacc gatccatcgt cgagaagaac tcgccacgtc
     1321 ccgtcgggaa cgggctcgag tcgctccacg gtgctgcgga acctgatgaa gcggcggtgc
     1381 ccgaagctct ccccgtaggc ctccaggtag gcggccatgt cgctgtggtg cgggaaatcc
     1441 ccgtacaccg tgggcatcgg gaagctcgga tactccatgc gaggtcgcga gacgttcgtc
     1501 cgaagcgacg cgtacgccga cgagaccccg ttgtcgttct ggtagcgcca cagcccgccg
     1561 gcctgcgaac cgcgctcgaa gcagcggacc gtcactccgc ggccccgcag cgctctgagg
     1621 accgcaagtc cggaggaccc cgcgccgatc acggctactc cttccacacc cgatccacct
     1681 gccagcatcc ttcttggctc cctcacatgc gtctgcaaga tcccgcctgc ggaatgcgac
     1741 gagggcggcc gcgacgagcg caccggctat caccgtgagg atcaactccg gaacgacgct
     1801 caactcctcc accggcatcc ttgggacgtg ctccagcggc gagagatcga agacccaatc
     1861 ggggagccga agcagcggcc ccatgaatcc gacgaaggcg agcgctccca ggacccccca
     1921 cgccgcgccg acgaaccttg gggccactcc gaacagcgcc gcagccagtc cgacgaagac
     1981 ccagagcgcc ggcgcggacg caagcgacgc ggcgatcaga cgcggaagct cgcccggatc
     2041 ctccgcgttg atcgatcccg tgatcccgag cccgagcacc aggagcacgg cggcgctgcc
     2101 gcccaacggg atcgccaatt gactcagggt cccgcgcacg cgcccgacgc gcgttgccag
     2161 atccgactcg gcttgctggg gcggcctcct cggtgcgaag gcgcaacgcg gactggatga
     2221 cgaaccccgt cgcgatcaac gccgagatga acgtccacag tgccaagaac tgggcgacga
     2281 gatcggcggc ccccagccga acaactccgc ggatttcatc cacaggcgcg tcggggtcgc
     2341 ggagtatgcg ggccaaccgt gccgtcggag tgtgaccgac cgccgatccg cccccgcgca
     2401 ccggcgacac gaccttcctc gtcccttggt tcttcgcgat cacccttcgt tcgatctggg
     2461 cgggggcctg cgcgaccagg tgctccaggc gtccgttcat ccgagggctt ccgacccagg
     2521 tcgggggcgg gtgctcctcg tcgcgtcact ctgcgtccgt gtctctgggg agccgtggca
     2581 gcgcagacgc tcgcgcctgt tgctgccggg acgacggatc gcctccgccc gaagggcttc
     2641 cgggtcatgt gggtcaacgg ggaggacggt cagccacccg cgctccctcc ggcgcttcgg
     2701 cctttcgaca cggatgcgct tcaccgcggc ctccctccgt gctcctcggg cggcagtcgc
     2761 cgcatgccga tcaccgagag ccccatgtct cgcaccttgt ccagtactcc gtgtaaggcg
     2821 gactgatcgg cgagcgttcc gaacaagatg gtttgacccg cttcggtgaa gacgctgaga
     2881 ccaccgaacc agtccgacca ccgttcctcc aagacgccct ccacacggat ctcgtaacgt
     2941 gccggccaaa gctcaccgct cacgttcgat cgcctgcctc gccgaagacc atcgaggtcg
     3001 cccgtgcgaa ccaggaggtc agccggactc gagcggagcg cagcggaccg gccgatcgac
     3061 gccgtcggtc cgtccgggtg aaccgcatgt ccggctgccc atgttcagtc aggacacggc
     3121 ggacaaggcg cccagcttcc gcttctccgt gcagctcagc gatccgctgc ctcagctcga
     3181 tcaacgacgc catcgatcac tctgcctttc ccatgacgtg tcacggtaag gaacggctgt
     3241 cggcgagaca tcacacgaag gtgcaacagc tggtgggatc ctttggaacg ccgacagcct
     3301 tgccggcgct gcggaagagt ggctgcccgg cgccagccgg cgggtgggag caggcggtcg
     3361 aagtgcccga gttccaagtc tgcgatcgcc cgcttcccgt gagagcacgc gaggcggcgc
     3421 gcgactaacg ttggtatcac cgatgtgagg tccgggggcg agacgtggtc ggaactcggg
     3481 ggtcacgaga ccatccgact cacgaacggg aagagcttct cgcgaccaag ctcgctaccc
     3541 cgcacgtccg cccgggactc cttgcccgtc cacgcctcct gaagtccctc gacgaggcga
     3601 tcgaccgggg gctgaccctg gtgtgcacgc cggcagggtt cggcaagacc actctgctcg
     3661 ccgactgggc ggaccgggtg aagcggcccg tggcatggct ctccctggac cccgacgaca
     3721 gcgacccggt tcggttctgg cgctacgtgg cagcggcctt ggagcgctcc ggcgtgcgtg
     3781 tcggagagcg ggtccgttcg ctgctcgagg ccccgagggg cgtgtcgagc gagggcgtgg
     3841 tgaccgctct cctcaacgag ctcgaggcct cccccgatga ggtgacgctg gtcttcgacg
     3901 actaccacgt gatcgagtcg cggccggtcc acgacggcat gaccttctta gtgagccacc
     3961 tcccccctca gctgcacgtc ctgatggcca cccggagcga cccgcccctc cccctcccgc
     4021 ggcttcgggc caggggtcag atcgccgagc tgcgagccgc cgacctccgg ttcacgcctg
     4081 aggagtcggc cgccttcctg cgcgaggtgt ggggcctgga cctttctccg gaggcggtcg
     4141 tcgtcctgga gtcgcggacc gaaggctggg tcgtcgggct ccagctggcc gctctgtccc
     4201 tgcgggaccg gccggatccc gacgcgttcg tcgacgcctt caccggaacc catcgctacg
     4261 tcctcgacta cctaagcggc gaggtgctcg agcgacagcc cgagcgggtg aggacgttcc
     4321 tgctgcgaac ctcgatcctg gaacgactga gcggtgcgct gtgcgacgcg gtcaccggag
     4381 gttccgatgg ccaggccatg ctggaggacc tggagcgggc caacctgttc ctggttcccc
     4441 tggacgagga gcgtcgctgg taccgcttcc atcacctgtt ccgcgaactc ctccgcgccc
     4501 gactgcagca gaccgagatc agcgtcgtcc cggacctcca ccggagagcc gccgcatggt
     4561 gcgagcggca cgggctgatc gacgaggcgg tccaccatgg cctgcgatct gatgacgcgc
     4621 cttgggcggc ccgactcgtg gagcagcacc tggccgagac cctcggccgc gccgaggagg
     4681 cgcacgtcga gcgatggctc gcccagctcc cagacgacgc cgtcctgtct cgacccgcac
     4741 tctgcctggc ccaggggttg aggcagctct tcctcggccg attggactcc gtcgagcgtt
     4801 tcttggggca ggccgagcgc gtgttccacc acggacagga gtcaccagcg ttcggggtgc
     4861 tgacagaggt tggcaccgtg ggtgaggtgc cggcagcgat cgcgctcctt cgatccgagc
     4921 tcgcgggggc tcggggcgac cccgagggga tggccgagta cgcggcgtca gccctgtccg
     4981 agatggccga ggaagagcac gggcctcgct tctgggcgcg ctggctctct ggggccgagg
     5041 ccgagttgat gcgggggaga ctggcggagg ccgagagagc gtccgccgag ctgctggccg
     5101 agggccatgc ggcaccggac cccctccccg ggatggtgtc ccccctttcc tggctcggcc
     5161 aggtgcagca ggcacgtggc agcctcaccg cagccctgcg aacgcaccga gaagctctgc
     5221 gcctggccac cctgagctct caggccagga tgaaggggcc agccccgacg acgtacatct
     5281 tcacgtcggc gtaccaggag ggtctggcgc accttgggat cgctcaggtg ctctacgagc
     5341 gggatctgct cgaagacgcc ctccggcatg tggaagaggg tatcgagctc ggccgccagg
     5401 agacttggtt ccgagaacac gcgctggtca tttccgcttg gatccgcgac gccatgggac
     5461 aggcagagac cgcccttgaa gccatgaacg aggccagccg gctgcaggcg agccccgagg
     5521 tcaacagcct ctggcacccc gctgcctcag aacgagcgag gctcctagtt gcgcagggac
     5581 agaccgccga agccgagcgg tggaccgagg aacgcgggtt gacggaggac gacgatgtct
     5641 cctacctgcg ggaacgtgac cacctcgtac tggcccgggt gctgctagcc cggtccgagc
     5701 ccggacgggc gctccggctg gtggaacgcg tcgacgagct tgcccgctcc caggaccgga
     5761 tccgaagtct catccagatc cgggccgtgg ggtcgctcgc gatgcaggcg acaggcgatc
     5821 accaaggtgc cctcgccctc ctggcagatg ttctcgcgct ggggcggccc gagggcttca
     5881 tccgcgtctt cgcggatgag ggaccgccga tggccgccct tctgcagagc ctcatcaggg
     5941 ctcgtcggca cggaggccgc gcggcggcct cccgggcgga gcgtgaacac gtgaacaggg
     6001 tcgttcgagc gttccggtcc ccggttgggc acctggaggt ccctgggacc gccccggtgg
     6061 aagcggggcc gatcgagccc ctgacccggc gggagttgga ggtgcttgga ttgatggccg
     6121 cggggaggcg taaccagcag atcgccgatg agctggtggt cacgctcgac acggtcaagc
     6181 ggcatgtgag ccacatcttc gacaagctcg gcacggcgag ccgcacggaa gcaatcgctc
     6241 gggcacgtga gctccgcttc ctcccttaga tcgaacccat cagggacccg acgtctcgaa
     6301 catgcacgcg cgcgacatgt tcgtggcctc agacgcgagc ggccgcgatc cagcatccag
     6361 gcgatcgctc cgatcgagag aatgagcgtc gcacgcctcg ccgcggagca tcctcgcacc
     6421 cccggccatg ttcgccgacc cgtggcggcg caggttacat gcctgaacgt cggaggggct
     6481 cgaggagtgc atcgaggtcg ggcgtggacc ggccggtgtt ccggacggcg cgtttgagcg
     6541 cgctcgccat gtcgtccatc tcttgaggtg cggcgctggt gtgaccgaac cagagctcgc
     6601 cgttggggct cgccaagacg cgtcgccagt agccggccgc gaacgcggtc ggtagccggt
     6661 agagcattcg caggttgccc ggtatctcgg ctgctcctgt cgcggtgagc gccccgaaag
     6721 cctgccgggt ggctcgcacc atctggcgca gaagaccgcg gtcggcggca agccgcgcag
     6781 ggtcggcatc cacgcgctgg agcgcgaagg cgatcggaac gacgaaggcg gcgtgcccga
     6841 gcagccagcc ctcgatgtcc cggctgatcg ccgtcggaaa gcctgcctgt atgagcagcg
     6901 cctcgagtga gcgcatccgt tcggaaggcc tacctgttgg ctcgccgaga gtggtcttct
     6961 gctggcggat gaggacgtag cggacgacgt gaccgtcgag gacgccgccg acgccgggga
     7021 agccgaacag gcagcggtcg ccgagcgcat cggcgagctc gcgggtgcgg ccggccgtgt
     7081 tgccgaagaa aagcacgtcg tgctcccccg agatgacctg cacgagcggg agcacggagt
     7141 cgagctgcgg tgcctgtacc acgatccaga cgaggtcgta acgctcaccg gggtcggttg
     7201 cgccgatcgc gcgcaccgcg agctcgtcgc gctcgccggt ctcggcgttc tcgacggtta
     7261 ggccgtgctc ggtgagctcg atgaggcgct cgccgcgggc gaggaccgtg atgtcgtggc
     7321 cgacgtgcag gaggcgggcg gagtagacac tgccgatcac gccagcgcca agcatgagga
     7381 tcttcacgcg cttcctccgg gccgacagat cgccgcggca aggaacagac cgaccacgaa
     7441 cactcgagga cgactcgtgg ctcgcttcat cgcgtcctct ctcggcagcc ttcgaagaac
     7501 actcctgttg tcgtgcataa cctcaggaac ggatcggccg atcatctacg accccgtggt
     7561 ctcgaacgcc gtcaggtagc gcggatctcg gctcacgtcg tccgagacga tcgtgtgttg
     7621 agcggcgacg gcgaccccgg tgagcccctt cgtcacgggg aatcgtgggt atgcgggcat
     7681 gccggaccca ggtggaggcg atcagcagaa cctcctcctc tgccacatcg aacacgccga
     7741 cccatcggta tccgcgccat gcccgcaacg cctcggctgt ggccgcggcc tttgcggcgc
     7801 gatcgccttc gctctccagg ctcagcctta cagcagtgag caactctttc gatgtctcct
     7861 cgatgcccat caaggtccca tctccccatg cggccctcgt tccgtccagc tctcacctct
     7921 cagcttcgcc ggtacctggc caccgcgagc ggcgcgaaca ccgcgacgat gccggccgcc
     7981 cagagcaacg ccctggtcac gaagtagctt gccggatgtc ccagcagcgc ctcggcctcc
     8041 gggcccaggg tgagcgcccg gacggcgtcg accatgtacg tcagcggctg gtgttcggcg
     8101 aacgcctgca tccagcccgg catcgactcc acggggacgt aggcgctcga gacgaacgag
     8161 agcgggaaca cgaggagcgc catcccctgc gccgcctcgg ccgagcccgc gaacaggccg
     8221 atcgtgatga acacccagat gaacgcgaac ccgaacgcga cacacagcgc gaacgcaagc
     8281 agcgcatcgg gaaccgagcc gtggatccgg aagcccaccg cgaagcccgt gagggtcatg
     8341 aacgcgaggc tgtacacgag caccgcggta tcggcgatcg cccgccccga gaggaacgag
     8401 ctgcggggga tgggaagcga ccgcaggcgg tcgacgaacc cctgctcgag atcgcccgcc
     8461 acgcccggag ccgcatccat caccgcgaac aggatcccgg tcgtgatgaa ccccggcacg
     8521 aggaagtcga cgtagctgat cccggggatc tcgatcgcac cgccgaacac gtaccggaag
     8581 atgagcagga acatcgcgcc ctggatcgtc ccgacgacca ccagctgcgg cgttcgcacg
     8641 aacttcatca acccccgttt cgccaccacg agcgtgctgg tgaccaggcc ggtggtgcga
     8701 cggccggtcg ctcggtccat cgtcgaagcg atcgttgcca tggccgtaac tcctccctcg
     8761 ctcaggccgc gtctgtcctc gactggcggc cggtttcctc gtgcaagggc tgcccggtca
     8821 gagccaggaa cacctcgtcg agcttcggtt ggcggagcgc gacgtcgtcg acctcgacac
     8881 cgaggtcgtg gatcgcacgc agccccgcgc gaaggggatc gccaccgggg tccaccgtca
     8941 cgctgacgcg acgggtcgac tcgtcgacct ggggttcccc gtgatcgagc ctgccgaagg
     9001 cctcggcgat cgcgaccaag tcctccgccc gccgggcgtg cacctcgatc acgcttccgc
     9061 cgatcctccg cttgagctcg gccggcgtcc cggaggccac cgcgcggcca cgatcgatga
     9121 tcacgatgcg actcgcgaga tggtcggcct cgtccaggta ctgggtcgtg agcagaacgt
     9181 cggtccctcg ctcaacgagc cggcggatcg aatcccaaag ctcgatccgg ctgcggggat
     9241 ccaggccggt cgtcggctcg tcgagcagga gcagccgggg ggctccgacc aggctcgcgc
     9301 cgagatcgag cttccgtcgc atgccgcccg agtacgtccg cacgagccgg ccggcctcct
     9361 cctggagccc gagctcctcg agcacggcgg cggcgttcgc gcgcgctgct cgtctgggct
     9421 ggccgagcag ccttgcgacc atctcgaggt tctcgcggcc ggtcatggcc ggctcaaccg
     9481 cggcgaactg acccgcgagc ccgatcaggc gtcgcaccgc ctccggctcg cgtcgaacgt
     9541 cgtagccggc cacgcggagg gagccgtcgt cgggccgcag cagcgtggcg acggcccgca
     9601 cgaacgtcgt ctttccggct ccgttcgggc cgagcacggc caggacggct ccgggctcgg
     9661 ccacaacgtc cagcccgtcg agcgcgacgg tccttccgaa acgcttgcga agcccgtggg
     9721 cctcgatggt gggggtcatg tcttcagccc tccttcgttg ggtcgtccac ggtgatccac
     9781 aggtgcgccg cgccgccgga gatctcgatc gcataccgat cgtcggcgag gcccgcgtcg
     9841 agcgtctcga ggtgaagacc gccgcccgcc gcgtcgctcg gctccgcggg cgatgtcgat
     9901 cgcctcaacc tcgacgtcac gtaggtccgc atccagatgc gcgacgccgc ggatcgacag
     9961 ggcccaggtc gcgtccgtgc tgagggccac ccgcccccgc cgggacctcc acggcccgac
    10021 ccagctccga ggtgagatcc gggataggcg aacgcgatcg ttccgccctt caccgcgacg
    10081 gcgggttcgg tcgggtttag gcgcgcccga acagctccgg gatctccgct ccggcgatcc
    10141 gcaggcgcgc ggcgccccgc gggaactcca gacgggcggc ggttgatccc ctcgcg
//
LOCUS       DASUYD010000198         5403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1891251, whole genome shotgun sequence.
ACCESSION   DASUYD010000198 DASUYD010000000
VERSION     DASUYD010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1891251"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..681)
                     /locus_tag="VF195_10895"
     CDS             complement(133..681)
                     /locus_tag="VF195_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEX6581365.1"
                     /translation="MDLAARIEQLDELLREAKSMPLSSSVLVNRDEILELIAEMKEGL
                     PEEIKRARWIVRDREELLAKARQDGEQIVEQAHEEQLRMARKEEIVARATREADRVVA
                     EAEERARVMRSEAEDYVDAKLAQFENSIRKILEASQASTKSLNRTLDQVEVGRERLRG
                     PATVAEAEFRTEHDLFDEEGDR"
     gene            complement(681..1166)
                     /gene="coaD"
                     /locus_tag="VF195_10900"
     CDS             complement(681..1166)
                     /gene="coaD"
                     /locus_tag="VF195_10900"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEX6581366.1"
                     /translation="MGLTALCPGTFDPVTNGHLDIIGRASQTFETVVVGALENPSKQP
                     MFTLEERVAMLKEACSGMSNVTVVSFRGLLVDLARSQEGDAAIVKGLRAVSDYEYEIQ
                     MAQMNHRLAGVETLFMPTSPKWSFLSSSLVKEVAQLGGNVAGLVPDHVRTALDDRLEE
                     R"
     gene            complement(1166..1759)
                     /gene="rsmD"
                     /locus_tag="VF195_10905"
     CDS             complement(1166..1759)
                     /gene="rsmD"
                     /locus_tag="VF195_10905"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEX6581367.1"
                     /translation="MRVIAGTVKGARLGRAPAGVRPVSDRVREGLFSSIGPWIEGSRV
                     LDLYAGTGAVGIEALSRGAEHATFVDSSPSAVAAVRDNLRRVGFEGRGAVRRSNVRTF
                     LERAGGPGFDLVFLDPPYEAGPAELDPVLAALDLSSLLREGFTVVISRGSRSSNGVIP
                     LHWEAARRLSYGDSVVSLFRSRIPPGTPRDMPGNLEV"
     gene            complement(1796..4042)
                     /gene="recG"
                     /locus_tag="VF195_10910"
     CDS             complement(1796..4042)
                     /gene="recG"
                     /locus_tag="VF195_10910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEX6581368.1"
                     /translation="MVEPFTNVPGSPSALALDSPVLHVDRKVATRRTGLRNKGEEAHV
                     ILAGMGIRTVGDLLRHYPRRYIDRSAVERIGDLQIGEPATVIARVQKTMKRLTRRRQS
                     MVTVTLADGSGTLDLTFFNQPWAAGIYKEGLELAVSGTVTRYRGRLQLANQEAEILGG
                     DERELVHTGRITPVHRASDGIATRTIRELVFFALERLPSIPDPIPPGMIEPEGLEDLD
                     SALRRVHFPEDAEQLARAIERLKFDELFTLELGVALRKHRLESERTGIAHQADGVLTD
                     RLLATLPFEPTKAQTRAIEEVGAAMAAARPMNVLLQGDVGSGKTLVAVSAGLVAIQSG
                     HQAAIMAPTEVLATQHARSVAALLGRVGAIDFLDRGSSGAPAPHGQSSLLEEPAVTPS
                     DDPVTYALLTAAVTGKDRARILAGIAGGDVDLVIGTHALVQEAVSFADLSLAVVDEQH
                     RFGLHQRMALKSKGEGDIDVVIMTATPIPRTLALTYYGDLDVVVLDEMPKGRQPIGTA
                     AARSDAERAAAYDLVRREVREGRQAFVVCAAIDEGNRSQVRAAEAEAERLATAIFPDL
                     RVELLHGRMRPKDKDRVMDDFRTGRSDVLISTTVIEVGVDVPNATVMLVENAERFGLA
                     QLHQLRGRIGRGAYDSYCVLFDESDETNQDARARIEAMTRTTDGFELADEDLRLRGEG
                     TLFDTKQSGMPDLKLARLAEDLDLVKRARTRAFELIEVDPELQDHPALLAELRERFED
                     SIAWLFSA"
     gene            4049..4585
                     /locus_tag="VF195_10915"
     CDS             4049..4585
                     /locus_tag="VF195_10915"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX6581369.1"
                     /translation="MTCRRLIAVVAGLLLLVACDGEPMDPPPQGEIPADNATETPLLP
                     VFADTLPRMDPDTFERLLAQLDGTPVLVNFWGSWCSPCEDEMPRLVGAHDEFGDRVQF
                     LGVDIIDSRKAATAFMAEYGMTFPSVFDPPDVIKTSLGQFGQPTTVFFRSDGTFEFAY
                     AGPIPEDVLLRHLERIAG"
     gene            4615..4818
                     /gene="rpmB"
                     /locus_tag="VF195_10920"
     CDS             4615..4818
                     /gene="rpmB"
                     /locus_tag="VF195_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003785965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEX6581370.1"
                     /translation="MAAVCDICGKKPWFGKSVTFSHKRNNRRWDPNIQRVRALVDGTP
                     GRINACTSCIKAGRVTRIPQRQP"
     gene            complement(4821..>5403)
                     /gene="thiD"
                     /locus_tag="VF195_10925"
     CDS             complement(4821..>5403)
                     /gene="thiD"
                     /locus_tag="VF195_10925"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010988600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX6581371.1"
                     /translation="LASAAIVEAVAESAAEMSLPNIVVDPVFVSKHGHPLLADDAVDA
                     LRRWILPLATLVTPNLAEAAGLAGLEVVTRDDMRRAATTILALGPRAVLVKGGHLEGD
                     RATDLFVDGDREVWLEADRIDTPHTHGTGCTLSSAITAHLARGASLADAVREGKAFVT
                     EAIRHALALGDGIGPVDQLWSIGVVREDGSEAR"
BASE COUNT          855 a         1909 c         1773 g          866 t
ORIGIN      
        1 ggtcggccgt gaccggcgta tccgctggca gaccggcgag ctccgtttcg agatcgtcga
       61 tcgtgccgga gacctgctcg gaccgccaca cgccaggccg tcccacgagg tcgcgcacat
      121 cgatcgcgcc gatcaccggt ccccctcctc gtcgaagagg tcgtgctccg tccggaactc
      181 cgcctccgcg acggtcgccg gtcctcggag ccgctcacgg ccgacctcga cttggtcgag
      241 cgtccggttc agcgacttcg tcgatgcctg cgaagcctcg aggatcttac ggatcgagtt
      301 ctcgaactgc gcgagcttgg cgtcgacata atcctcggcc tccgagcgca tcacgcgggc
      361 acgctcctca gcctccgcga ccacccgatc cgcctcgcgc gtcgcccggg cgacgatctc
      421 ctccttgcgt gccatccgga gctgctcctc gtgcgcctgc tcgacgatct gctcgccgtc
      481 ctggcgcgcc ttcgcgagca gctcctctcg gtcccgcacg atccaccgag cgcgcttgat
      541 ctcttccggc agcccttcct tcatctcggc gatcagctcg aggatctcgt cgcggttgac
      601 gagcacggac gaggacaatg gcatcgactt cgcctctcgc aagagctcgt cgagctgctc
      661 gattcgggcc gcgaggtcca tcagcgttcc tctaaccggt catcgagagc cgtccgcacg
      721 tggtcgggga cgagtcccgc gacgttcccc ccgagctgag cgacctcctt cacgagcgag
      781 gacgagagga atgaccactt cgggctcgtg ggcatgaaca gcgtctccac cccagcgaga
      841 cggtggttca tctgcgccat ctggatctcg tactcgtagt ccgagacggc gcggagtccc
      901 ttgacgatcg cggcatcacc ctcctgggaa cgggcgagat caacgaggag accccggaac
      961 gagaccacgg tgacgttgga catgccggag cacgcctcct tgagcatcgc gacccgctcc
     1021 tcgagcgtga acatcggctg cttggacggg ttctccagcg caccgacgac caccgtctcg
     1081 aaggtctgcg atgcacgacc gatgatgtcg agatgaccgt tcgtcacggg gtcgaacgtc
     1141 cccggacaca gggcggtcag gcccatcaca cctccaagtt tcctggcatg tcccttgggg
     1201 tccccggcgg gatccgtgac cggaacaacg agacgacgct gtctccgtag gagagccgcc
     1261 tcgcggcttc ccagtgtagc ggaatcacac cgttggaact cctcgatccg cggctgatga
     1321 ccacggtgaa accctctctg agcagggaac tcaggtccag ggcggccaga accggatcca
     1381 actcggcggg gcccgcttcg tacggcggat cgaggaagac gaggtcgaaa cccggcccgc
     1441 cggcccgctc gaggaacgtc cgaacgttcg accgacggac cgcgcctcga ccctcgaagc
     1501 caactcgccg aaggttgtcg cggaccgcgg cgacggccga aggggagctg tcgacgaacg
     1561 tcgcatgctc cgctcccctg gaaagggcct cgatgccgac cgcacccgtt cccgcataga
     1621 gatcgaggac gcgcgaacct tcgatccacg ggccgatgct ggagaagagg ccttcacgga
     1681 cgcgatcgga cacgggtcgg acgcctgccg gcgcgcgccc caggcgcgcc cccttcacgg
     1741 tgcccgcgat gacgcgcatc gcacgaggat acggggaggg gcagaagccc tctcctcacg
     1801 cgctgaacag ccaggcgatc gaatcttcga accgctcgcg caactcggcg aggagcgcgg
     1861 gatgatcctg aagctccggg tcgacctcga tcagctcgaa cgcgcgggtt cgggcgcgtt
     1921 tcacgaggtc gagatcctcg gcgagacggg cgagcttcag gtcgggcatg cccgactgct
     1981 tcgtatcgaa cagcgtgccc tcgcctcgga gcctgaggtc ttcatccgcg agctcgaacc
     2041 cgtcggtcgt cctggtcatc gcctcgatcc gggcgcgcgc atcctggttc gtctcgtcgg
     2101 actcgtcgaa caggacacag taggagtcgt aggcgccgcg gccgatccgc cctcggagct
     2161 ggtggagctg cgcgagaccg aatcgttcgg cgttctcgac gagcatcacg gtcgcgttcg
     2221 ggacgtcgac gccaacctcg atcacggtgg tggagatcaa cacgtcggag cggccggtgc
     2281 ggaagtcgtc catcacacgg tccttatcct tcggccgcat ccgtccgtgc aggagctcga
     2341 cgcggagatc ggggaagatc gccgtggcga gccgctctgc ttcggcttcg gccgcccgca
     2401 cctgcgaacg attcccctcg tcgatcgcgg cgcacaccac gaaggcctgc cgtccctctc
     2461 gcacctcgcg ccgcaccagg tcgtaggcgg cggcgcgttc cgcgtccgag cgggccgccg
     2521 ccgtcccgat cggctgacga cccttcggca tctcgtcgag caccaccacg tcgaggtcgc
     2581 cgtagtacgt gagcgccagc gtcctcggga tgggcgtcgc cgtcatgatc acgacatcga
     2641 tgtcgccttc gcccttgctc ttgagcgcca tccgttggtg gagaccgaac cggtgctgct
     2701 cgtccacgac cgccagggag agatcggcga aggacaccgc ttcttgcacg agggcgtggg
     2761 tcccgatcac gaggtcgacg tccccgcccg cgatcccggc gaggatccgg gcccggtcct
     2821 tcccggtgac ggcggcggtg agcagcgcgt acgtgaccgg gtcgtccgat ggtgtcaccg
     2881 cgggctcctc gagcagtgaa ctctggccgt ggggcgccgg cgcgccgctc gacccgcggt
     2941 cgaggaaatc gatcgcaccg accctcccga ggagggccgc gaccgagcgg gcgtgttggg
     3001 tggcgaggac ctcggtgggc gccatgatgg cagcctgatg gcccgactgg atcgccacga
     3061 ggccagccga cacggccacc agcgtcttcc cggaaccgac gtcaccctgc aggaggacgt
     3121 tcatcggacg agcggcggcc atggccgccc cgacttcctc gatcgcgcgg gtctgggcct
     3181 tcgtgggctc gaagggcagg gtcgcgagga gccggtcggt cagcactcca tccgcctgat
     3241 gcgcgatccc ggtcctctca gactcgagcc ggtgcttgcg gagcgcgaca ccgagctcca
     3301 acgtgaacag ctcgtcgaac ttcagccgtt cgatcgcgcg cgcgagctgc tccgcgtcct
     3361 ccgggaagtg cacccgtcgc agcgccgaat cgagatcctc gagcccttcg ggctcgatca
     3421 tgccgggagg gatcgggtcc gggatcgacg ggagccgctc gagcgcgaag aacaccagct
     3481 cccggatcgt ccgggtggcg atcccgtcgg aggcacggtg gaccggggtg atccgtcccg
     3541 tgtgcacgag ctcccgctcg tcgccaccga ggatctccgc ctcctggttc gccagctgga
     3601 gccgaccccg atatctggta acggtgcccg acacggcgag ctccaagccc tccttgtaga
     3661 tgccggccgc ccacggctgg ttgaagaacg tcaggtcgag cgtgccgctg ccgtccgcga
     3721 gcgtcaccgt caccatcgac tgccggcgac gcgtcaatcg cttcatcgtc ttctgcacgc
     3781 gcgcgatgac ggtcgctggc tcaccgatct gcaggtcgcc gatccgttcg accgccgaac
     3841 ggtcgatgta gcgccgcggg tagtgccgga ggagatcccc gaccgtccgg atccccatcc
     3901 cggccaggat cacgtgcgcc tcttcgccct tgttccgcag gccggtgcgg cgggtcgcga
     3961 ccttccggtc cacgtgcagg accggggaat cgagcgcgag ggcggaggga gaaccgggca
     4021 cgttcgtgaa cggttctacc atccacgtgt gacgtgtcga cgcctcatcg ccgtcgtggc
     4081 cgggctcctg ctcctcgtgg cgtgcgacgg cgagccgatg gatccgccgc cccaggggga
     4141 gatcccggcc gacaacgcca ccgaaacgcc cctgctcccg gtgttcgcgg acacgctgcc
     4201 caggatggat ccggacacgt tcgagcgtct cctggctcag ctggacggca caccggtgct
     4261 cgtcaacttc tggggatcgt ggtgctcgcc ctgcgaggac gagatgccac gtctcgtcgg
     4321 agcgcacgac gagttcggcg accgggtgca gttcctcgga gtggacatca tcgactcacg
     4381 gaaggctgcc accgcgttca tggcggagta cgggatgacg ttcccgagcg tcttcgatcc
     4441 accggacgtg atcaagacct cgctcggcca gttcgggcag ccgacgacag tgttcttccg
     4501 atcggatgga accttcgagt tcgcctacgc gggtcccatc cccgaggacg tcctgctccg
     4561 gcacctcgag cgcatcgcgg ggtagcgggc ccctctggta gcctcgtcgc acctatggca
     4621 gcggtctgtg acatctgtgg caagaagccg tggttcggca agtcggtgac gttctcgcac
     4681 aagcggaaca accggcgatg ggacccgaac atccagcgcg tgcgcgcgct cgtcgacggg
     4741 acgcctggcc gcatcaatgc gtgcacgagc tgcatcaagg ccggccgagt cacccgcatc
     4801 ccgcaacgcc agccctagac tcagcgcgct tccgaaccat cctcgcgcac gaccccgatc
     4861 gaccacagct ggtcgaccgg accgatcccg tcacccaacg cgagggcgtg gcggatcgcc
     4921 tccgtgacga atgccttccc ctcgcggacc gcgtcggcca gcgacgcccc tcgagccagg
     4981 tgcgcggtga tcgcggagga caacgtgcat ccggtgccgt gcgtgtgagg cgtgtcgatc
     5041 cgatcggcct cgagccacac ctcccggtcc ccgtcgacga acaggtcggt cgcgcgatcg
     5101 ccctccaggt gccctccctt cacgaggacg gcgcggggtc cgagcgcgag gatcgtcgtc
     5161 gccgcacgcc tcatgtcgtc tcgggtcacc acctcgagcc cggccagtcc ggccgcctcg
     5221 gcgaggttcg gtgtgacgag tgtcgcgagg ggcaggatcc atcgccggag ggcgtcgacg
     5281 gcgtcgtcgg cgagcaacgg atgcccgtgc ttcgacacga agacggggtc gacgacgatg
     5341 ttcgggagcg acatctccgc ggccgactcc gccacggcct cgacgatggc ggccgaggcg
     5401 agc
//
LOCUS       DASUYD010000199         6882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_350066, whole genome shotgun sequence.
ACCESSION   DASUYD010000199 DASUYD010000000
VERSION     DASUYD010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6882
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350066"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF195_10930"
     CDS             complement(<1..555)
                     /locus_tag="VF195_10930"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581372.1"
                     /translation="MITLIGEPSTELLERQGELDALAALLDDVREHGRGWLVLVAGEA
                     GVGKTALLRRFCDEHRSSARILWGACDALYTPSPLGPLIEVAESSGGEVEALVHSAAK
                     PYEVATALMHELGRHAPSVLVLEDVHWADEATLDVVRLLGRKVEGIPALVLVSYRDEL
                     DRVHPLRVLAGELSTSQAVRRMRVD"
     gene            complement(613..1785)
                     /locus_tag="VF195_10935"
     CDS             complement(613..1785)
                     /locus_tag="VF195_10935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581373.1"
                     /translation="MTEQQRPRLPARAEVVGSLLRPRALREAIDTFYERGHSAVLVEE
                     RAKDRATLTEVENAAIRETVQRQIDLGLDVVTDGEFRRWMFLNSFYDAVSGVRTDKAV
                     TFRNARGEDVELAVHEIVEPLKQVDSPAAREAAFLSEVTHGYPFKVSLPAPSIFLHPF
                     TTVTGAYESVEEFADEAIQIERQLVRDILSAVPAQTHTSYTMQFDFPLYPYLVDPTWV
                     ARFEARGHPVADLVDAAIAADTAVLEGIPDEVTVALHICRGNFRSSWMCEGSLEPVAE
                     RVFGELPYDAFLVEWDDLGRDGGFEPVRYLRGGSTMVMGILSTKTPVLEAEDDLLRKM
                     DVAAGFVDGGLDRLAISPQCGFASVMVGNEIDEGTQWRKLELVVGVADRIWGRSST"
     gene            complement(1834..2607)
                     /locus_tag="VF195_10940"
     CDS             complement(1834..2607)
                     /locus_tag="VF195_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581374.1"
                     /translation="MASIKLLRTFKDLDLRVRNSLADEESPMNLAFAALAIAHDDAGV
                     DYVSLDDLHEALQEADVAVDRSSLARGIGRAGKRVNRRIIDGEQVYRLVLRGRPAAEI
                     VLGSGDLSVLHIDGTTPRTDRRALGDVFGGLKGIVRVCDPYYGVCSLDSLELIPKSAQ
                     VRFITGRTTESPTKWAGALKDFKKERPNVELRVAARPQDLHDRYVLTDAKILIIGHGL
                     KDIGNKESFVVILLRAVAGDLLGDVQRSFDDKWGRATQL"
     gene            complement(2645..2965)
                     /locus_tag="VF195_10945"
     CDS             complement(2645..2965)
                     /locus_tag="VF195_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581375.1"
                     /translation="MSGSRTVPAAESSNLAVRTRRRKLKSPKAKTKTAKAKRSGPTDY
                     IRELISDGYFKSEREIGDVMDKLKERAHIYPVTSISGPLYRLVKTKELRRSKKDGAWK
                     YANW"
     gene            complement(3466..4578)
                     /locus_tag="VF195_10950"
     CDS             complement(3466..4578)
                     /locus_tag="VF195_10950"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX6581376.1"
                     /translation="MMRTRHGRWLALAAVLTLGACTSAREADAPPAAEASSTVDAGTT
                     APSPHAFPIAALGDFPQFPEGPLPEPVGGLLQAVLDGAAEDGAVGGVTAAVIVAGSGS
                     WSGAAGVDLEQNSLIPDAQLPIASAGKTIIAAQVLRLAEQGKLGLDDHAADHLPRELK
                     SFDTNDATVRHLLGMRSGIPDLPDIMEPLPTATEFLKTFTDVRHFPAGSRIEYSNTNF
                     ELLELIIEAIARRPIEEVLRDDVLSGPGLGALRQTANDSIEFYGVSESDSASLARWGY
                     QLYGGSVLSDASLREMTDFRGDWYGLGAIDYSLGSPLEAGYGIPAVGHGGLGPSASVA
                     FVAFPETALVLAVQATIESLGPVHTIVRALKAAVEA"
     gene            complement(4643..6010)
                     /locus_tag="VF195_10955"
     CDS             complement(4643..6010)
                     /locus_tag="VF195_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581377.1"
                     /translation="MPETSPLLERVMERVELRPFTLEGFHDRRDHKRRNQRIRAGVLG
                     IAIAIAVGWLSVNAIRSAPPVPADPTPTPELRRDGEVLVFEPRATGKGWDLAAQDPQT
                     GEVRTIAETDEIIDCPDAERCRSFVREAEWSADGRWLALEVTHANLDGPAIGPCAPTV
                     GVWVTKALGELRLLTTPCDAPAPDAVEEVWEWSPVGARLAYARVDGETDELFVIDPSD
                     GGRMSLGTADIEPRNGTAASPGWALEWSPDGTRIAYADGGSVYAVDADGGEPSLLADS
                     FEEIIEIAWSPDGTHILVHDQGRYRLQVMNADGSDLHVLLEGEDACCETEWSPDGDRI
                     LYMLSVVRPGQAPYGLFDSEVWTVSPDGSNAIKVFDSDGCDMDPREMVSGGDALPVWA
                     PNGTQVAYNACGVWVVANADGTGEARPLGGLAPADLVVPLLHRSWNGGGLSQWDLALI
                     GQVDH"
     gene            complement(6003..6329)
                     /locus_tag="VF195_10960"
     CDS             complement(6003..6329)
                     /locus_tag="VF195_10960"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX6581378.1"
                     /translation="MDDLAGYLHRTAMNLFRSQYRRARVALKRAALSTPERDVFEAVE
                     ERDAATQALARLTPRQRAALVLTEALGYSGEETGRLLGIKASTVWALTHQARTALKGT
                     LEANDA"
     gene            6688..6882
                     /locus_tag="VF195_10965"
                     /pseudo
     CDS             6688..6882
                     /locus_tag="VF195_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017587995.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
BASE COUNT         1134 a         2401 c         2163 g         1184 t
ORIGIN      
        1 atcgacccgc atccgcctca ccgcctggct ggtcgagagc tcgcccgcca ggacccgcag
       61 cggatgcacc cgatcgagct cgtcgcggta gctcacaagg acgagagccg gaataccttc
      121 caccttgcgc ccaagcaggc gcaccacgtc gagggtcgcc tcgtccgccc agtgaacatc
      181 ttcgagaacg agcacgctcg gcgcatgcct gccaagctcg tgcatgaggg ccgtggcgac
      241 ctcgtagggc ttcgctgcgc tgtggacgag cgcctccacc tcgcccccgc tcgactccgc
      301 gacctcgatc agcggcccca gcggactcgg cgtgtagagc gcgtcgcagg caccccaaag
      361 gatccgagcc gagctccgat gctcgtcgca gaagcgccgc aagagcgccg tcttcccaac
      421 cccggcctcc cccgcgacga gcacgagcca accacgccca tgctcacgca cgtcatcgag
      481 caacgcggcc agcgcgtcca gctcgccctg ccgctcgagg agctccgtcg agggctcgcc
      541 gatcagcgtg atcatgcgtc agggcatggt catccaacgt ggttgaccac gcaagtgccg
      601 cgtttaagac cgctacgtcg acgaccgacc ccagattcga tcggcgaccc cgacgacgag
      661 ctcgagcttg cgccactgcg tgccctcgtc gatctcgttg cccaccatca cgctcgcgaa
      721 cccgcactgc gggctgatcg caagccggtc aaggccgccg tctacgaacc cggccgccac
      781 gtccatcttc cgaagcagat cgtcctcggc ctcgaggacg ggtgtcttcg tggagaggat
      841 ccccatgacc atcgtcgaac cccctctcag gtagcgcacc ggctcgaacc caccgtctcg
      901 tccgaggtcg tcccactcga ccaagaacgc gtcgtacggg agctcgccga acacgcgctc
      961 ggcgacgggt tcgagcgagc cctcgcacat ccacgacgaa cggaagttcc ctcgacagat
     1021 gtgcagcgcc acggtcacct cgtcggggat gccctcgagc acggcggtat cggccgcgat
     1081 ggcggcgtcg acgagatctg cgacggggtg gcctcgcgcc tcgaagcgcg cgacccacgt
     1141 cgggtcgacg aggtacggat agagcgggaa gtcaaattgc attgtatagc ttgtgtgagt
     1201 ttgggctggt actgcggaga ggatgtcacg gacaagctga cgctctatct gaattgcctc
     1261 atccgcgaac tcctcgaccg attcgtaggc acccgtcacc gtcgtgaacg gatgaaggaa
     1321 gatggagggt gccggtaggg agaccttgaa cgggtacccg tgggtcacct ccgaaaggaa
     1381 cgccgcctcg cgagccgccg gtgagtcgac ctgcttgagg ggttcgacaa tctcgtggac
     1441 tgccagctct acgtcctcgc cgcgagcgtt gcggaacgtc actgccttgt ccgttcgaac
     1501 tcctgatacc gcatcgtaga aggagttcag gaacatccac cggcggaact cgccgtcggt
     1561 caccacgtcg agcccgagat cgatctgtcg ctggacggtc tcacggatcg cggcattctc
     1621 gacctcagtc agggtcgcgc ggtccttcgc gcgctcctcc accaggacgg ctgagtgacc
     1681 ccgttcgtag aaggtgtcga tggcctctcg cagagcgcgc ggccgcagca aggagccgac
     1741 gacctcggca cgggcgggaa gacgaggcct ctgctgctca gtcacgcgac caacgttcgc
     1801 tggaggtttc tggtttggtc ggacatgtgt gttctaaagt tgcgttgccc tcccccactt
     1861 gtcgtcgaag gagcgctgca cgtcaccgag cagatcgcca gcaaccgctc gcagtagtat
     1921 cacaacgaac gactccttgt tcccgatgtc cttcagaccg tggccgatga tcaggatctt
     1981 tgcgtcggtc aagacgtacc ggtcgtgcag gtcttggggg cgcgctgcca cgcgcagctc
     2041 cacgttcggc cgctccttct tgaagtcctt gagcgcgccc gcccacttcg tcgggctttc
     2101 agtcgtcctc cccgtgatga accgaacctg cgccgacttc gggatcagtt ccagactgtc
     2161 gagcgagcac acgccgtagt atgggtcgca cactcgcacg attcctttga gcccgccgaa
     2221 gacatcgccc agcgcccgcc ggtcagtgcg cggcgttgtg ccgtcgatgt gaaggaccga
     2281 gagatcccca cttccgagca cgatctcggc ggctggtcga cccctgagaa cgagtcggta
     2341 tacctgttcg ccatcgatga tcctgcgatt gacgcgcttg cccgcgcgac cgatccctct
     2401 cgcgagggat gacctatcca ccgccacgtc ggcttcctgc agagcttcgt gcagatcatc
     2461 cagggacacg tagtccacgc cggcgtcgtc gtgcgcgatc gcgagcgccg cgaacgcgag
     2521 gttcatcggc gactcctcgt cggcaaggct gttccgcaca cgaaggtcta ggtccttgaa
     2581 cgtcctaagc agcttgatcg acgccacgtt acgccttctt cgtgtacgtc acggcaaggc
     2641 tccgctacca gttcgcgtac ttccaggccc catccttctt gctccggcga agctccttgg
     2701 tctttaccaa ccgatagagg ggaccggaga tgctcgtgac tggatatatg tgcgcgcgtt
     2761 ccttgagctt gtccatgacg tcaccgatct cgcgttccga cttgaagtag ccgtcgctga
     2821 tcagctcacg aatgtagtcc gtcggccccg agcgcttcgc tttagctgtc ttggtcttcg
     2881 ccttagggct cttgagcttg cgacgtcgcg tgcggacggc gaggttgctg gactctgcgg
     2941 cgggcaccgt ccgagaaccg ctcaagcagc ccagcaacct cgtcggccgt tccctcgatc
     3001 gccacctttg caccgccggg gagcaccagg ttggcctttg ccatcagctc cagactcctt
     3061 gttcaagcgt cgatgactaa ggctacgatt cgaataaggc ctcggcaagc atccctccgc
     3121 cacggccggc tcgccgtgtg gcggtgcctt ctaagaagga cccgaccgct cgccctcccg
     3181 cagcatctcg acgagcaccg cccggagtgt ccgatcctcc tcggcgtcac tcagcagttc
     3241 cgcgaacgtc cgcgcttcgg gttcccccag aactctccga tccggtcggc ccgcacgaag
     3301 tcggggacat aaggacgtgg agcagttcgg cgcggggagc agggccgagc gcgtccagac
     3361 gctccggtat cggctcgaag tcgtcgtgac gcattgagta cgagcctgct tcttgagccc
     3421 gatcggagga ttctgtgccc ttcagggccc gatcctccgc agagctcagg cctccactgc
     3481 agctttcaag gctcgcacga tcgtatggac gggtcccagg ctttcgatgg tggcctgcac
     3541 cgcgaggacg agggccgtct ccgggaacgc cacgaacgcg acggaagccg atggtcccag
     3601 tccgccatgc ccgacggccg ggatgccata gcccgcctcg agcggcgaac ccagcgagta
     3661 gtcgatcgcc ccgaggccgt accagtcacc ccggaagtcg gtcatctcgc gcagggaggc
     3721 gtcggagagc accgacccgc cgtacagctg gtagccccac cgcgccagag atgcggagtc
     3781 cgactcgctc accccataaa actcaatcga atcgtttgcc gtctgccgga gggcaccgag
     3841 tccggggccg ctcaggacgt cgtcccgcag cacctcctca atcggtcggc gtgcgatcgc
     3901 ctcgatgatc agctcgagca gctcgaagtt agtgttcgag tactcgatcc tcgaacccgc
     3961 cgggaagtgg cggacatccg tgaaggtctt caagaactcg gtcgccgtcg gtagcggctc
     4021 cattatgtcg ggcaggtccg ggatgccgct ccgcatgcca agaaggtgcc ggaccgtcgc
     4081 gtcgttcgtg tcgaacgact tgagctccct cggaagatga tccgcagcgt ggtcatccag
     4141 tcctagtttc ccctgctcgg ccaggcgaag cacctgcgcg gcgatgatcg tcttgcccgc
     4201 gctggcgatg gggagctgag cgtccggtat caaggagttc tgctcgaggt cgacgcccgc
     4261 ggcccccgac cagcttcccg agccggcgac gatgaccgcg gccgttaccc cgccgaccgc
     4321 gccgtcctcc gccgcaccgt cgagcaccgc ctggagcaag ccgcccacgg gttcggggag
     4381 aggcccctcg gggaactgcg ggaagtcccc gagggccgcg atcgggaaag cgtgagggct
     4441 tggggccgtc gtgcccgcgt ccaccgtcga gctggcctcc gccgctggcg gagcgtccgc
     4501 ctcgcgcgcc gatgtgcatg ctccgagcgt gagcacggcg gccagcgcga gccaacgtcc
     4561 gtgacgggtc ctcatcattg ccgctccctt cttgatcgga cggcctggca tcccggatcg
     4621 acgcccacgc ggatcagcac gcctaatggt ccacctgacc gatcagagca aggtcccact
     4681 gggagagccc gccgccgttc cagctcctgt gcaggagggg cacgaccaga tccgcaggcg
     4741 cgagcccgcc caaaggtcgg gcctcacccg ttccgtcggc gttcgcgacc acccaaacgc
     4801 cgcaggcgtt ataggcaacc tgcgttccgt tgggtgccca gaccgggaga gcatcgcccc
     4861 cagataccat ttccctggga tccatgtcgc agccgtctga gtcgaagacc ttgatcgcgt
     4921 tcgagccgtc cggcgagacc gtccagacct cggagtcaaa caacccgtac ggggcttgtc
     4981 cgggccgaac gacagagagc atgtagagga tccggtcccc gtccggtgac cattccgtct
     5041 cgcagcaggc gtcctcaccc tcgagcagga catggagatc cgacccatcg gcgttcatga
     5101 cctggagccg gtaccggccc tgatcgtgga ccaggatgtg cgtgccatcg ggagaccagg
     5161 cgatctcgat gatctcctcg aacgagtccg cgagcaacga cggttcccct ccatccgcgt
     5221 caaccgcgta gacggagcct ccgtccgcgt aggcgatccg cgtgccgtcg ggcgaccact
     5281 ccagcgccca cccaggcgac gctgcagtcc cgttccgagg ttcgatgtcc gccgtcccca
     5341 gggacatccg accgccgtcc gaggggtcta tgacgaagag ctcgtccgtc tcgccgtcga
     5401 cccgcgcata ggcgagccga gcgcccacgg gcgaccactc ccacacctcc tcgaccgcgt
     5461 cgggcgccgg agcgtcgcac ggggtggtca gcagtctgag ctcaccgagc gccttcgtga
     5521 cccagacccc gaccgtcgga gcgcatggac cgatggccgg gccgtcgagg ttcgcgtggg
     5581 tgacctccaa cgcgagccag cggccgtcgg ccgaccactc ggcctccctg acgaagctcc
     5641 gacaacgttc ggcgtccggg cagtcgatga tctcgtcggt ctccgcgatc gtgcggacct
     5701 cgcccgtttg cggatcctgg gcggccaggt cccacccttt gccggttgcc cgtggctcga
     5761 acacgaggac ctcgccgtcc cggcggagct cgggcgtcgg cgtcgggtcg gccgggaccg
     5821 gcggcgccga tcggatcgcg ttgacgctga gccagcccac ggcgatcgcg atcgcgatgc
     5881 ccagcacccc ggctcggatc cgctggttcc ggcgcttgtg gtcgcgacgg tcgtggaatc
     5941 cttcgagcgt gaacgggcgg agctcgacgc gctccatcac gcgctcgagg agcggggagg
     6001 tctcaggcat cgtttgcctc cagggttcct ttcaatgcgg ttcgcgcttg gtgggtgagc
     6061 gcccacacgg tcgacgcctt gatgccgagt agccgaccgg tctcctcacc ggagtagccg
     6121 agggcctcgg tcaggacgag cgcggcgcgc tgtcgcggcg tgagccgggc cagcgcctgt
     6181 gtggcggcgt cacgttcctc caccgcctcg aacacgtctc gctcgggcgt tgagagggcg
     6241 gcgcgcttca gtgcgacccg cgctcgccgg tactggctgc ggaacagatt catcgcggtc
     6301 cgatggaggt agccggcgag atcgtccatg tgcccgacca cgtcccaagc tcgaacatgc
     6361 gggtgaacgc ctcctgcgcg aggtcctcgg cttcgccccg gctcccggtg atttaccgag
     6421 agcacgcgga gtaggcggtc cttctcgtcc tcgaagaacg cctcgaagct cggcggctcc
     6481 tgccgggggc catcgacccc tcgggagtct ccgatctccg tcgcagccga gccacctcgc
     6541 ccatctcgag gattcaacac ctgagagggg cgagacctgt aggggaatgg ccgcagagtt
     6601 cttcggtgcg aaggccttac ggggaccgat cggcctcccg cagcatcccg acgagcaccg
     6661 cccggagtgt ccgatcctcc tcggcgtgac gatccgcgcg gcgtggtctc cgtccgaaga
     6721 ctctccaagg ccagttaccg aaagatgctg cagaacctcg ggtgggcggt ggggtacaac
     6781 gtgatcgcca tccccctggc tgccggggtc ttcgcgtggg cggggttggt gctcgcgccc
     6841 gccgtcggcg ctctcgccat gagcatctcg accatcgtcg tg
//
LOCUS       DASUYD010000200         2670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1234191, whole genome shotgun sequence.
ACCESSION   DASUYD010000200 DASUYD010000000
VERSION     DASUYD010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1234191"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(571..951)
                     /locus_tag="VF195_10970"
     CDS             complement(571..951)
                     /locus_tag="VF195_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581379.1"
                     /translation="MKKAIAFGLVTAFVLMVPAGIARAGQNDVIREGSCGGASDWKLK
                     VSSEDGGLEVEFEVDQNVSGDRWRVRIRHDGDLAFRGTRTTRGASGSFEVRIVENDTS
                     GSDVFRGRARNLSTDEVCVGSATF"
     gene            complement(1310..1624)
                     /locus_tag="VF195_10975"
     CDS             complement(1310..1624)
                     /locus_tag="VF195_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581380.1"
                     /translation="MPPTYELARTYIVIERGEVAPFVLAEVVGCAVEPEAGLDPGRLP
                     WSSLTTNAEEMNSNSAGSVYTRGQLEADPRLKRALERWEAGDDSPAAEYERRILIQYE
                     AS"
     gene            2466..2670
                     /locus_tag="VF195_10980"
                     /pseudo
     CDS             2466..2670
                     /locus_tag="VF195_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182253.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          436 a          919 c          812 g          503 t
ORIGIN      
        1 cggtatctgt ttcgatcgcc gacccgggcc ctatgtcgat ccgaaccagc actcgtcggc
       61 tgaacatcag gaagaggtca tgggggccgc cgaccaactg cccgtatcga cactgagggg
      121 aaaagtccga tcagcaacgc cgtggcgccg cgaatcgcct ccagcgactt cagccgttcg
      181 cgctcggtcc gcgccctcgc cgcaaactcg aaggcggccg cgagtggatc gaccggccca
      241 ctttggccgg cctcaccgcg gatcaccccg agaccgcgcg aggtcccccg cgcgtgctgc
      301 cgatggcggg ccagggtaga ccgcgcgacc cgtgtaggcg ggacacggac cagttggacg
      361 agccggcggc gagcaggacg tcgatctcgg cgactgcgga atggctacat aagctgcagc
      421 ttctcatcgg gtctccgtgg atcggatatt actcctagca actgaccggg ggtgtcccgc
      481 gccttgacgg cgccctgcgt gtgcggccga atccactgaa cacgggaagg agcccctcga
      541 cgatcgcggg gctcctcggg aacgaccgac ctagaaggtg gcgcttccca cgcacacctc
      601 gtccgtcgac aggttccgcg cccgtccgcg gaagacgtcc gagccggacg tgtcgttctc
      661 gacgatccgg acctcgaacg acccgctcgc gccccgcgtg gtgcgtgtgc ctcggaaagc
      721 gagatcgccg tcgtggcgga tcctgacccg ccagcggtcg cccgagacgt tctggtccac
      781 ctcgaactcg acctcgagcc caccgtcctc cgatgagacc ttcagcttcc agtcgctcgc
      841 accgccacac gacccttccc ggatcacgtc gttctgacca gcccgcgcga tcccagcggg
      901 caccatcagc acgaacgccg tcacgagccc gaacgctatc gccttcttca tcccgacctc
      961 cttcgatcgt tgatggaacc gagcctacgg agggggtcga taactgcggg taaaggcgag
     1021 ccgcgctctc ggtaaaggtt cgccgctatc cttgctcgcg gacgtaatcc tggggccttg
     1081 caggacctcg atatgcggtc tcgcataact gctggatccg tcatccagga tcgccgttcg
     1141 gcgttgtgat gagtgatgac aactcatgca cctgcaccca cgacggctcg aacgggcgcg
     1201 atctccctct cgggactgcg aacactcccc gggcggatcg acccgatgta cgccttcgac
     1261 gtcttcgttg atctccctcg aggcggcgtc gttctgcgcc atccgctcct cacgaggcct
     1321 catactggat gaggatgcgc cgctcgtact cggctgctgg ggaatcgtcg cccgcctccc
     1381 agcgttccag ggcacgcttg aggcgtgggt ccgcttccag ctgcccccgg gtatagacgc
     1441 tccctgccga gttgctgttc atctcctcgg cgttagtcgt gaggctcgac cacggcaggc
     1501 ggcccggatc gagccccgcc tcgggttcca ccgcgcaacc aacgacctcg gcgaggacga
     1561 acggcgcgac ctccccacgt tcgatgacga tgtaggttct tgcgagctcg tacgtcggtg
     1621 gcatcgtgct cacctcccga ccggccggga ctgccgaccg gtcacccctt atcgtcggcg
     1681 agcgtcgccg ccgcgacccg ggtcggacga cccggtcccg ataggccgtg ggggcagtcg
     1741 gggcgatccc cccgctcggc ggcctgtgtg ccggggatta ctacctacca tctcgactcg
     1801 ccggaaactc catcggagtc tccgctcttc gctcttcatg ctgtcggtgt cttcgcgtcc
     1861 gaaggtttga ccgtgtcgtt gcttgggccc gttctcgtgt tccggttccg ctggatcgtt
     1921 gttgtcggcc tcggttggat cgtcctcgtc gcccacagtc cctcccgctg tcacccgaag
     1981 tgtcacccga cgcgcgagcc tgagcggccg aaaatccctc tgacctggag cggacgacgg
     2041 gattcgaacc cgcgacccca ccttggcaat tgcatggcgg atgtctcatg cgtctccgcc
     2101 ggtctcacca gtgccgctga gctgcgcgtt cttggcgctc ttgtctcatc cgtctcacca
     2161 gatcgctgga gtcgactcga tttcgttggt gatttcgttg gtgctccgcc acgcccgatt
     2221 cgcgaaggcg aaggggacca gcggatatga gtttgatcag gcgctctccc caaccaggta
     2281 gaatgagatg agagagtcgt tgaccagcgc tttctctccc cagagccgcc catcgttaag
     2341 caatgtttcg cggtccgtcg cggggccaat gcgggacgca tcatgctctg gactcgccga
     2401 acgtcgtgaa tccgagttgc tatgcattcg cacaagtgag cgcgtcaggc ccaacagggg
     2461 cctcctacca cgtgctggac cgggggatca gccacgtcta catcaagcct gcgaccccga
     2521 ggctgaacgg gaaggtcgag cggtcccacc ggatcgatgc cgaggagttc tatcggatgc
     2581 tcgacggggg ttgtgatcga tgacaccggc ctgttcaaca ctcgcctgaa agagtgggag
     2641 gacttctaca acttccatcg accgcatgga
//
LOCUS       DASUYD010000201        15645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_793401, whole genome shotgun sequence.
ACCESSION   DASUYD010000201 DASUYD010000000
VERSION     DASUYD010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15645
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793401"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VF195_10985"
     CDS             complement(<1..1234)
                     /locus_tag="VF195_10985"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6581381.1"
                     /translation="MKKVGRWLVVGAALALAAAACGDEPTDPGAGDDVTTFDEIGEGE
                     GQLNLIAWHGYTEDGTTEGYEEYDWVTPFEDETGCQVEVTYGDSSDEMVQLMRQGGGS
                     EFDGVSASGDASNRLIAGGDVGAVDPSLFPEMENVIAPLNPEGGTNTDHYIVGDNVFG
                     TPYMYGPNFLMYNTEEVTPAPTSWDVTFEADSPYAGKVTAYDSPIFIADAAMYLMTTQ
                     PDLGITDPYELTQEQFDASVDLLKQQAEIISAYWALYTDEIDGFVDGSMVVGTAWPVN
                     LTYTKGPEAANTAAVIPSEGVTGWADTWMISANAPHPNCMLKWMDWTLQPEIQAEVGI
                     YYGAAGSNVDSCAEIATQLGKGGEDLVDTVEYSFCGDAEFLDSIHLWKTPQVACGDDR
                     GDTCIDYSQWTQAWTEVRG"
     gene            complement(1231..2352)
                     /locus_tag="VF195_10990"
     CDS             complement(1231..2352)
                     /locus_tag="VF195_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020122254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581382.1"
                     /translation="MVEAARRDKAHAETAGAPVAVALDDVVKRFGDVVAVDGVSLDVR
                     PGEFFSLLGPSGSGKTTCLRMIAGFERPTSGRIFLDGREVSDLPPFERDVNTVFQDYA
                     LFPHMTVAQNVGYGLMVRKLPAGDRSRRVSDALEMVRLEHLGDRKPGQLSGGQRQRVA
                     LARSLVMRPKVLLLDEPLGALDLKLRQAMQIELKEIQQEVSLTFLYVTHDQEEALTMS
                     DRLAVFNQGKIEQVGTPAEVYERPATAFVAGFIGVSNVLEGDVAARITGSDHAFTVRP
                     EKIAIVPLESERGASHDTTTGRVREVVYLGAVTRYIVELDGGGELVVMQQNLTTSSME
                     ALQVRGKAVGLQWDRSNNRPVDTTGGNEGSSEDPEEGAV"
     gene            complement(2447..3445)
                     /locus_tag="VF195_10995"
     CDS             complement(2447..3445)
                     /locus_tag="VF195_10995"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase 2"
                     /protein_id="HEX6581383.1"
                     /translation="MGRRDGQRDGWYGTVGRPAFFALPPEASHRVATSLLSLPLPWER
                     IGGVVDDPSLEVDLCGIRLRNPIGLAAGFDKTCRHLGPLGRLGFGFVVGGTITRASRK
                     GNAKPRIVRATERRAIVNSMGLPNPGAEVAAENLSRALGTASRFASVADEDLAMAVET
                     VELLAPHVDGIELNASSPNAPWRHDPAHMSRLLEAFRGRTDRPLLLKLPPFTTDEERA
                     TVLALARAAVEAGAHGLVCSNTLPIDEPRLATGRGGLSGGPLTERTPRTVSDVAAATG
                     RAVPIVACGGIFTSEDVRRCLDAGASAVQLYTSLIYRGPGIVGSLTRGLLPGRAPA"
     gene            3466..4023
                     /locus_tag="VF195_11000"
     CDS             3466..4023
                     /locus_tag="VF195_11000"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6581384.1"
                     /translation="MTTTPPPTGDDLVRRMVAASELRGDFVLSSGQRSSVYFDKFRFL
                     TDPTLLRDVAKATATLLPTGVTVLGAPEGAAMLLVAAVSLETGLPVTVVRKQAKDYGT
                     RAQVEGVVEAGARVALIEDVSTTGHQVRAAAEVLEAAGAEIVAILLAIDRGGADLLRE
                     AGYEVRAVAVLRPENEAPENSVPEN"
     gene            complement(4020..4130)
                     /locus_tag="VF195_11005"
     CDS             complement(4020..4130)
                     /locus_tag="VF195_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX6581385.1"
                     /translation="MRALTRSSSGAWFSGTEFSGTEFSGTWFSGTEFSGT"
     gene            complement(4118..4819)
                     /locus_tag="VF195_11010"
     CDS             complement(4118..4819)
                     /locus_tag="VF195_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEX6581386.1"
                     /translation="MSEVNWTGRTGPFTFRVNPGVFAPTHTSKTIAEALEVDPGDTVI
                     DVGCGSGVLSFVAARLGAQRVVGCDISEEAVEMARLNARALGLEERCEFRVGSLLDPV
                     RDLRADVLIGDVSGIPDEIAQVAGWFPDGRAGGPTGAELPSAMLESIGETLKRGGHLY
                     LPTGTIQDERTILAKARELFGERMQAVIERDFPLPDVVGKSRAAARMMKDGLLNLHKR
                     GSRFLWRLRVWRCER"
     gene            complement(4841..5353)
                     /locus_tag="VF195_11015"
     CDS             complement(4841..5353)
                     /locus_tag="VF195_11015"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX6581387.1"
                     /translation="MPSGDDLSRTEAAGKTGKPTETRNKPERPRDRLGRPLPWGSDSD
                     LELEDFDALSLDENHELGRTHARSGRWFPAHEAWETAWKQARDTAEAELFKGLSQMGA
                     GYVHLLRGNAHGASRLLRRAAGRIESYEDGTRGVPTTALAARLLADADAVDRGDLAPG
                     EHAPFAPVAV"
     gene            complement(5363..6655)
                     /locus_tag="VF195_11020"
     CDS             complement(5363..6655)
                     /locus_tag="VF195_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX6581388.1"
                     /translation="MDATRGPGETRVVMEPGPTTVETTIDVPEPERETWRRRWPPMSY
                     WIRLLLTIAAVSAALVALWSVINIVILVMMSLVLAIGLDPAVRWIERRGRSRGRGVTI
                     IFLGALAVVILFASLVIPPLVTQVGELADDIPGYVERLSRRDDALGRYFQENDVSDQL
                     QAFVKDLPKKITTSFGTIVGVAGKVGGALFNVLTVAILTIYFMLSLPRMRRTAVVWFA
                     PETRERAEAVMDQAILRIGAYVAGILTTASIAGTSALVFFWILGLFDIGIPFAVPLAV
                     FSGLLGLIPAVGAYIGAAPAVIVGFFQEPITGVLILIYFIVYQQVENYVIQPRIMKNA
                     VNLSPVAVVISTLVLGSLAGFAGAVLALPAAATMKVILVEVFLRDRVAEGDPAAQEKL
                     EEHDRAEAEAQADARQRAHARRRLVERMRDRFGRTPPG"
     gene            6678..7781
                     /locus_tag="VF195_11025"
     CDS             6678..7781
                     /locus_tag="VF195_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6581389.1"
                     /translation="MEHVDVVIVGGGPMGTAAARALSARGRAVILLERFTLGHARGST
                     AGTTRNFRLTYHDPLYVRMARRSLERWRELEDEAGVELLRVVGGLDVGAATEASARAL
                     EHAGERLERPSDADVAERWPMLRFPPRSSFVYQPDGAVLRSQEAVRTIARLATAHGAE
                     VREETAAESIAASGKGVEVVTASSTIRAPVAIVAAGAWSGSLLGRAGIPLTLRPSLEQ
                     STYFDADATDLPTVIDWDEAPEQPPYIVPDPFEPGTIKAGAHLSGPAVDPDTRSFDAD
                     EARELQVADWVGRRLVSGARPLRTETCLYTRTPDEDFVLDRVGPLVVASPCSGHGFKF
                     TPLIGEVLADLVTGEAPEISLERFRADRPALRG"
     gene            complement(7788..8222)
                     /locus_tag="VF195_11030"
     CDS             complement(7788..8222)
                     /locus_tag="VF195_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX6581390.1"
                     /translation="MTEPQLGRQLLAAHRTLAAELVVELEERGWPELRASQASLVLNV
                     DRRSGTRLTELARRAGVTKQAMMVVVDELEVRGFVRRTPDPDDGRAKVVRLTARGRTL
                     GAECRRAVAAVEARTRRTLGGRRYEGLREALDLLLETEEETA"
     gene            8318..8836
                     /locus_tag="VF195_11035"
     CDS             8318..8836
                     /locus_tag="VF195_11035"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6581391.1"
                     /translation="MEIREATPAEYAEAGRVTAEAYREYVRPGEDDWERYLLRIADVR
                     ARADQTTIMVAVENGRVLGSLTLELDGRVRDGDQEHRPLDPGEAHIRMLGVDPAARSR
                     GVARALMASSEARAYEAGKTFMTLHTTQRMGAAQAMYERLGYERIEDRVYPDGFVLLT
                     YEKQLDVSRSRT"
     gene            complement(8979..10949)
                     /locus_tag="VF195_11040"
     CDS             complement(8979..10949)
                     /locus_tag="VF195_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581392.1"
                     /translation="MGRHGFFRVSPIDEDHRKVDRATVRRVVGTFRPYKGKVGLVGLM
                     IGVTSGLGVINPLMIVLVFDEALFGPNGVCQGRPCPNLPLLYLYVGIMIAVPIVTSII
                     GIGQTYLTNVVGLNVMQDLRNRLYQHLQAMPLRFFTTTRTGEIQSRLANDVGGVQSVV
                     TDTASTVLSNVVTIISTIVAMLLISLPLTILSLLLLPLFLWLTVKVGRARREVATNTQ
                     RTLADMTTITEETLSVSGILLSKAFGRQRFESDRFRGENERLTGFLLRQTMIGRSFFA
                     LVGTFFSITPALVYLVAGWVNSSGGRPVIAAGMLVGFTTLQSRLFFPIGSMLQVSTEV
                     QSSLALFDRIFEYLDLPHEIHDAPDAVRIHDVKGRVRLRDVRFRYEDPPPEERGSIPV
                     SPDASALGDDDPVPREWTLDGVDLDIRPGQLAALVGPSGAGKTTVTYLVPRLYDVQEG
                     AVEIDDLDVRKITLESLGEAIGVVTQETYLFHDTVRRNLQYGKPDATDEELVTAAKAA
                     NIHERIAELPEGYDTVVGERGYKLSGGEKQRLAIARVVLKDPRILILDEATSSLDTTS
                     ERLVQSALEPLMKGRTTIAIAHRLSTILSADVIFVLDRGRIVERGTHDELLEHGGLYA
                     QLYEQQFRGGLVQAEFEDGVILASGEVVRTES"
     gene            complement(11003..11584)
                     /locus_tag="VF195_11045"
     CDS             complement(11003..11584)
                     /locus_tag="VF195_11045"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEX6581393.1"
                     /translation="MRIVRPIVVLGAVIVATLSLGLVSAAAPPACTWTGTPGPDVKHG
                     TPGDDVLCGMGGDDKLYGGRGDDRLRGGWGDDFLTGEGGDDIIHGQQGRDTIIGRGGN
                     DRMHGGNGIDDGDGGIGDDVVDGGPGRDLTLYGGSAGADRVYGGDGNDFCVTTIDSSG
                     NDAVFGGAGVDHYWSDPGDDVTNAEIFGPCFAE"
     gene            11705..12175
                     /locus_tag="VF195_11050"
     CDS             11705..12175
                     /locus_tag="VF195_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEX6581394.1"
                     /translation="MGTFDEGRLSLELTLAARMGLAALLGFLIGLERESRGKSAGERT
                     FAIVALGAAAFSSTAITMFPDTAGQAVAGVATGIGFLGVGIIWRAEEGETRGLTTAAS
                     ILCVAAIGVVTGIGLYVAAILGTILALFILEFDRMPGLRGLHRRVTGRPDENDG"
     gene            complement(12144..12851)
                     /locus_tag="VF195_11055"
     CDS             complement(12144..12851)
                     /locus_tag="VF195_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6581395.1"
                     /translation="MSIDDSLERIHDEVVVCRACPRLVEWRERVAREKVARFADQAYW
                     GRPVPGWGDPGAAVLILGLAPAAHGGNRTGRIFTGDRSGDFLFASLHRTGFADQPASV
                     SVDDGLRLRGVYIAAVNRCAPPGNKPTPEERDRCLPYMEREIAVLRDLQVLVALGAFA
                     WDGALRALAALGHAVRPRPRFGHLMEASVGPFILLGCFHPSQQNTFTGKLTPQMLDEV
                     FHRARELASRRSRPAVP"
     gene            complement(12848..14479)
                     /locus_tag="VF195_11060"
     CDS             complement(12848..14479)
                     /locus_tag="VF195_11060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX6581396.1"
                     /translation="MSVVSLVGVHKSYGTWPVLQGVDFMVPDGARIGIIGPNGAGKST
                     MLRIVGGTEDVNEGEVVRRRGITVSYLEQNPEGDERTAEAWVLAGRPDIAALDDELRS
                     VEAELARPAVTADLAKMDRVLTKQQAVLDRWVEAGGPGLAGEARSLLVRLGFDEHDLS
                     LPTTALSGGQRKLAAFAACLIRDPGLLLLDEPETHLDSDKREILGDVVAEFPGAVITV
                     SHDRYLLDDTVNQIAELDRGAVTIWPGTYSTYAVAKELALQRQQRVWVTQQKEIARLE
                     AAIARFELWASWVPNERHIRQARNKQRQIDQMDKVDRPVFERRKMALELRPAERGGQR
                     IARLRDVSMAFDDDPVLLDVSFDVYRRERLGVVGPNGAGKTVLGRILAGELEATTGER
                     WIGPSIRIGYLEQSFQSQDPTLTPVGSVRKTKAMYEGEAVALLAKFLFRYDQMRTPVG
                     SLSGGERTRLELCLLMLSGANCLILDEPTNHLDIESMEVLEGAVESFDGTAILISHDR
                     YLLDRLCDRILEVRDGEVRSFDGGYDDWVAAKAAV"
     gene            complement(14481..14879)
                     /locus_tag="VF195_11065"
     CDS             complement(14481..14879)
                     /locus_tag="VF195_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581397.1"
                     /translation="MFRRKRLSPELDATLADLRAVVERVEEAKLAMTGTVPSTRSAGT
                     PLAEGILELEQRLAAAQELMPTWRHHELEDEWLACDAGIRESLDRARRLREDPPELGG
                     FEGLIWAVDQLLAPLEAFEAAAERFRTLRR"
     gene            complement(14880..>15645)
                     /locus_tag="VF195_11070"
     CDS             complement(14880..>15645)
                     /locus_tag="VF195_11070"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6581398.1"
                     /translation="AVPGQSGVSVERPARPMVVAHRGASVEQPENTIAAFEAAIDAGA
                     DVVEFDVRMTADGHAVVMHDLDVSRTTDGHGLVSDMTLEEIRRLGVPGLEEALRCLSG
                     RAAADIEIKNLPGEPGLTPAREAAVEATLEALDATGFAGQVIVSSFNPASIAHSRALR
                     PDVPTGLLTEYEVAAEEALARATSGGHPWILPFVSRVLDAGRGFADQVHDGGALLGVW
                     IADDPDTARRLFELGADAVATNDPRAIVPVRNETVL"
BASE COUNT         2418 a         5518 c         5248 g         2461 t
ORIGIN      
        1 ttccccggac ctcggtccag gcctgcgtcc actgggaata gtcgatgcag gtgtcccctc
       61 ggtcgtcgcc gcaggccacc tggggtgtct tccagaggtg gatcgagtcc aggaactccg
      121 cgtcaccgca gaacgagtac tccacggtgt ccacgagatc ctctcctccc ttgcccagct
      181 gggtcgcgat ctccgcacag gagtccacgt tggagccggc cgccccgtag tagatcccga
      241 cctccgcctg gatctccggc tgcagggtcc agtccatcca tttcagcatg cagttcggat
      301 gtggcgcgtt cgccgatatc atccacgtgt cggcccatcc ggtgacccct tccgaaggga
      361 tcaccgcggc cgtattcgcg gcctcagggc ccttcgtgta ggtgaggttc acgggccacg
      421 ccgtgccgac caccatcgag ccgtcgacga agccgtcgat ctcgtcggtg tagagcgccc
      481 agtatgcgct gatgatctcc gcctgctgct tgagcagatc gacggaggcg tcgaactgct
      541 cctgggtgag ctcgtacgga tcggtgatcc ccaggtccgg ctgggtggtc atcaggtaca
      601 tggcagcatc cgcgatgaag atcggggagt cgtaggcagt cactttgccc gcgtacgggg
      661 agtccgcctc gaacgtcacg tcccagctcg tcggagcggg ggtgacctcc tccgtgttgt
      721 acatcaggaa gttcggcccg tacatgtacg gcgttccgaa cacgttgtca cccacgatgt
      781 agtggtcggt gttcgtcccc ccctcggggt tcagtggcgc gatcacgttc tccatctcgg
      841 ggaacaacga cggatccacc gcaccgacgt cgccgcctgc gatcaaccgg ttggatgcgt
      901 caccggaggc ggacacgccg tcgaactccg agccaccacc ctgccgcatc agctggacca
      961 tctcgtccga gctgtcgccg taggtgacct cgacctgaca gccggtctcg tcctcgaacg
     1021 gggtcaccca gtcgtactcc tcgtagccct cggtggtccc gtcctcggtg tacccgtgcc
     1081 acgcgatcag gttgagctga ccttccccct cgccgatctc gtcgaacgtc gtgacgtcgt
     1141 cgcctgcacc tgggtccgtc ggctcgtcac cacatgccgc cgcagccaac gcgagcgcgg
     1201 caccgacgac cagccatcgt ccgaccttct tcatacagcc ccctcttccg ggtcctccga
     1261 ggacccctcg ttccctccgg tcgtgtctac gggccggttg ttacttcgat cccactgcag
     1321 cccgacggcc ttgccgcgga cctgcagcgc ttccatcgag ctcgtggtga ggttctgctg
     1381 catcacgacc agctcccccc ctccgtcgag ctcgacgatg taccgggtca cggccccgag
     1441 gtagacgacc tcgcggacgc gtccggtcgt cgtgtcgtgg ctcgcgcccc tctcgctctc
     1501 cagcgggacg atcgcgatct tctcgggccg gaccgtgaac gcgtggtcgc tcccggtgat
     1561 ccgggccgca acgtcgccct ccaagacgtt cgagacgccg atgaagccgg ccacgaacgc
     1621 cgtggcgggc ctctcgtaca cctcggccgg cgttccgacc tgctcgatct tcccctggtt
     1681 gaagaccgcc agccggtcgc tcatcgtgag cgcctcctcc tgatcgtgcg tgacgtagag
     1741 gaaggtcagg ctcacctcct gctggatctc cttcagctcg atctgcatcg cctgacggag
     1801 cttgaggtcg agcgcgccga gcggctcgtc aagcagcagc accttcggcc gcatcacgag
     1861 cgatcgcgcg agggccacgc gctgtcgctg gccgccggac aactgtccgg gcttccggtc
     1921 accaaggtgc tccaggcgca ccatctcgag agcgtcggac acccgtcgcg accggtctcc
     1981 cgcgggcagc ttgcggacca tcagcccgta gcccacgttc tgggccaccg tcatgtgcgg
     2041 gaagagcgcg tagtcctgga acacggtgtt cacgtcgcgc tcgaacggcg ggagatcgga
     2101 gacttcacgt ccgtcgagga agatccgccc cgatgtcggg cgctcgaacc ccgcgatcat
     2161 ccgcaggcag gtggtcttcc ccgatcccga gggcccgagc agcgagaaga actcgccagg
     2221 gcgcacgtcc aggctgacac catcgaccgc gacaacgtcg ccgaagcgct tcaccacgtc
     2281 gtcgagcgcc accgccacgg gtgcgccggc cgtctcggcg tgtgccttat ccctccgggc
     2341 agcttcgacc actcgccgct tccccctctc tgacccgcag cgtggcgaac ccggcaatct
     2401 tgaccgcgaa ggccgatgcg tgcaatgggg ttgcgtcgcc ggtcggtcag gcgggggcac
     2461 gtcctgggag caggccacgc gtgagggagc cgacgatccc cgggcctcgg tagatcaggc
     2521 tcgtgtagag ctgcaccgcc gacgctcccg catcgaggca ccggcgcaca tcctccgatg
     2581 tgaagatgcc tccgcacgca acgatgggga ccgcgcgccc ggtcgcggcg gccacgtcgg
     2641 agacggtcct cggcgtccgc tcggtcaggg gcccgcccga gagaccgccg cggccggtcg
     2701 cgagtcgtgg ctcatcgatc gggagcgtgt tcgagcacac gagcccgtgg gcgcccgcct
     2761 ccacggccgc ccgagcgagg gccagcacgg tcgcgcgctc ctcgtcggtg gtgaacggcg
     2821 gcagcttcag caggaggggt cggtccgtgc gccctcggaa cgcctccagc agccggctca
     2881 tgtgcgcggg gtcgtggcgc cagggcgcgt tcgggctgct cgcgttcagt tcgatcccgt
     2941 cgacatgggg agcgaggagc tccacggtct cgaccgccat cgcaaggtcc tcgtccgcca
     3001 cgctcgcgaa ccgcgatgcg gtgccgaggg cccgcgagag gttctccgcg gcgacctcgg
     3061 caccgggatt cgggaggccc atcgagttca cgatcgcgcg gcgctccgtc gctcggacga
     3121 tcctcggctt ggcgttcccc ttccgcgagg cccgcgtgat cgtcccaccc acgacgaagc
     3181 cgaaccccag cctcccgagg gggccgaggt gccggcacgt cttgtcgaag cccgcggcga
     3241 gcccgatggg gttccggaga cggatcccac agaggtcgac ctcgagggag ggatcgtcca
     3301 ccacgccacc gatccgctcc cacggcagcg gcagggacag cagagaggtg gcgacgcggt
     3361 gcgaagcttc gggagggagc gcgaagaacg ccggtcgccc gacggttccg taccagccat
     3421 ctcgctggcc gtcgcgacga cccacgcccg tatgctatcc gccctgtgac cacgactccg
     3481 ccgcccacgg gagacgacct cgtgcggcgg atggtggccg cctcggagct gcgcggcgac
     3541 ttcgtgctct cgagcgggca acggtcgtct gtctacttcg acaagttccg attcctcacg
     3601 gacccgacgc tgttgcgcga cgtcgcgaag gccaccgcga cactgctccc gacaggcgtc
     3661 accgtgctcg gcgctcccga gggcgccgcg atgctcctcg tcgcggccgt ctccctcgag
     3721 accggtctgc ccgtgaccgt cgtccggaag caggcgaagg actacgggac gagggcacag
     3781 gtggagggcg tggtggaggc cggagccagg gtcgcgttga tcgaggacgt gtcgacgacc
     3841 ggccatcagg tgcgggcggc cgcggaggtt ctcgaggcgg ccggagcgga gatcgtggcg
     3901 atcctcctcg cgatcgatcg cggaggcgcg gatcttctcc gggaggcggg gtacgaggtg
     3961 cgtgccgtgg cggtgctgcg gccagagaac gaggcgccgg agaactcagt gccggagaac
     4021 taggtgccgg agaactcggt gccggagaac caggtgccgg agaactcggt gccggagaac
     4081 tcggtgccgg agaaccaggc gccggacgac gagcgcgtca gcgctcgcac cgccagacgc
     4141 ggagacgcca caggaagcgc gatccccgct tgtgcaggtt caggagcccg tccttcatca
     4201 tgcgcgcggc agcgcgcgac ttgccgacca cgtccgggag cgggaagtct cgctcgataa
     4261 cggcctgcat ccgctcgccg aagagctctc gggccttcgc gaggatggtc cgctcgtcct
     4321 ggatcgtgcc cgtgggcaga tagaggtgcc ctccccgctt cagcgtttcc ccgatcgact
     4381 cgagcatcgc ggaggggagc tcggctcccg tcggaccgcc ggccctcccg tcggggaacc
     4441 agccggccac ctgtgcgatc tcgtccggga tccccgagac gtctccgatc aggacgtcgg
     4501 cccggagatc acggaccggg tcgagcaggc tgcccacgcg gaactcgcac cgttcctcga
     4561 gcccaagggc cctcgcgttc aagcgcgcca tctccacggc ttcttcggag atgtcgcagc
     4621 ccacgacgcg ttgagctccg aggcgggcgg cgacgaacga cagcacgccg gagccacagc
     4681 cgacatcgat cacggtgtcg cccggatcga cctcgagcgc ctcggcgatc gtcttgctgg
     4741 tgtgggtcgg ggcgaacacg cccgggttca cgcggaacgt gaagggaccc gtccgcccgg
     4801 tccagttgac ctcactcacg gcctggctca ccattgcagg ctacacggcc acgggcgcga
     4861 acggcgcgtg ctcccccgga gctaggtcgc cgcgatcgac agcgtccgca tccgcgagga
     4921 gacgggccgc gagcgccgtc gtgggaacac cccgggtccc gtcctcgtac gactcgatcc
     4981 gtccggccgc ccgcctgagc aacctggaag ccccgtgtgc gttgccccgc aggagatgca
     5041 cgtagccggc ccccatctgg gacaacccct tgaagagctc cgcttccgcc gtgtcacgcg
     5101 cttgtttcca cgcggtctcc caggcctcgt gggcggggaa ccagcgaccg gatcgagcgt
     5161 gcgtccgccc gagctcgtga ttctcgtcga ggctcagcgc atcgaagtcc tccagctcga
     5221 gatcgctgtc ggatccccac gggagcggtc ggcccagtcg atcgcgaggt cgctccggct
     5281 tgttccgcgt ctcggtgggc ttgccggtct ttcccgccgc ctctgtcctg ctcagatcgt
     5341 ctccggaggg cacacgatga tgctacccgg gcggcgttcg tccgaagcgg tcccgcatgc
     5401 gttcgacgag gcggcgacga gcatgcgccc gctgacgcgc gtcagcctgt gcctctgctt
     5461 cggcccgatc gtgctcctcg agcttttcct gcgccgccgg gtcgccttcg gcgacccgat
     5521 cgcgcaggaa gacctcgacg aggatgacct tcatcgtggc cgccgccggc agcgccagga
     5581 cggcaccggc gaatcccgcg agcgaaccga gcacgagggt agagatgacc accgcgaccg
     5641 gcgacaggtt gacggcgttc ttcatgattc gcggctggat cacgtagttc tcgacctgct
     5701 gatacacgat gaagtagatg aggatcagga cgcccgtgat cggttcttgg aagaagccaa
     5761 cgatcaccgc gggtgcggcc ccgatgtatg caccgaccgc cgggatgagc ccgagcagcc
     5821 cggagaaaac ggcgagcggt accgcgaacg ggatcccgat atcgaacagc cccaggatcc
     5881 agaagaacac cagggcggag gttccggcga tcgatgcggt ggtcaggatc ccggccacgt
     5941 acgcgccgat ccgcaggatc gcttggtcca tcaccgcctc ggcgcgttca cgcgtctccg
     6001 gcgcgaacca gaccacggcg gtccggcgca tcctgggcag cgagagcatg aagtagatcg
     6061 tgaggatcgc caccgtgagg acgttgaaga gcgcgccccc gaccttcccg gcgaccccga
     6121 cgatcgtccc gaaggaggtc gtgatcttct tcgggagatc cttcacgaac gcctgaagct
     6181 gatccgacac gtcgttctcc tggaaatagc gcccgagggc gtcgtcgcgt cgagacagac
     6241 gttcgacgta tcccgggatg tcgtcggcga gctcgcccac ctgggtgacg aggggcggga
     6301 tcaccaacga ggcgaagagg atcacgacgg cgagggctcc caggaagatg atcgtcaccc
     6361 ccctaccgcg ggaccggcca cgtcgctcga tccaacggac cgccggatcc aggccgatcg
     6421 ctaacaccag ggacatcatg accaggatca cgatgttgat caccgaccac agagccacca
     6481 gcgcggcgga gacggccgcg atcgtcagca gcagccggat ccagtaggac atcggcggcc
     6541 accggcgccg ccacgtctcc cgttccggct cgggaacgtc gatcgtcgtc tccacggtgg
     6601 tgggacccgg ctccatcacc acccgagtct cgcccggccc gcgggtcgcg tccactaccc
     6661 ggtacgctcc gcccgcgatg gagcacgtcg acgtcgtgat cgtgggaggc ggaccgatgg
     6721 gcaccgccgc ggcccgcgcg ctcagcgcga gggggcgtgc cgtgatcctg ctcgagcgat
     6781 tcaccctcgg gcacgccagg gggagcaccg cggggacgac gcgcaacttc cgcctcacgt
     6841 atcacgatcc tctctacgtc cggatggcga ggcgctcgtt ggagcggtgg cgggagctgg
     6901 aggacgaggc cggcgtggag ctgttgcgcg tggtgggcgg gctcgacgtg ggcgcggcga
     6961 ccgaggcgtc ggcgcgagcc ctcgagcacg ccggcgagcg cctcgagcgc ccgtcggacg
     7021 ccgatgtcgc ggagcgctgg ccgatgctcc gcttcccgcc gcgctcgtcc ttcgtctacc
     7081 agcccgacgg ggcggtcctt cgttcccagg aggcggttcg aaccatcgcc cgactggcga
     7141 ccgcgcacgg tgcggaggtt cgggaggaga cggcggcgga atcgatcgcc gcgagcggga
     7201 agggcgtgga ggtggtgacg gcgtcgagca cgatccgggc gccggtggcg atcgtggcag
     7261 ccggcgcgtg gtccggctcg ctcctggggc gggcggggat cccactgacg ctgcgaccga
     7321 gcctcgagca gtcgacgtac ttcgacgcgg acgccacgga cctgcccacc gtgatcgatt
     7381 gggacgaagc gcccgagcag ccgccgtaca tcgttcccga ccccttcgag ccgggcacga
     7441 tcaaggcagg cgcacatctg tccggcccgg ccgtcgatcc ggacacgagg tcgttcgacg
     7501 ccgacgaggc gcgcgagctt caggtcgccg actgggtggg acggcgactc gtgtccggtg
     7561 ccagacccct ccgcaccgag acgtgcctct acacgaggac gcccgacgag gacttcgtcc
     7621 tcgatcgggt cggtccgctc gtggtggcat ccccgtgcag cggccacggt ttcaagttca
     7681 cgccgctcat cggcgaagtg ttggcggatc tcgtcaccgg tgaagcgccc gagatctccc
     7741 tggaacggtt ccgagccgat cggccggccc tccgaggctg aggcggctca cgcggtctcc
     7801 tcctcggtct cgagcaggag gtcgagcgcc tcccgcaatc cttcgtagcg ccgccctccc
     7861 agcgtcctcc tcgtgcgagc ctcgaccgcc gcgacggccc ttcggcactc ggcgccgagc
     7921 gtccggcccc gcgccgtcag ccggaccacc ttcgcccggc cgtcgtccgg gtccggtgtg
     7981 cggcgaacga accctcgcac ctcgagctcg tcgacgacga ccatcatcgc ttgcttagtg
     8041 acccccgccc ggcgggcgag ctcggtcaac cgggtgccgg agcgccggtc gacgttcagc
     8101 accagcgagg cctgggacgc tcggagctcg ggccatcctc gctcctcgag ctccaccacc
     8161 agctctgcgg cgagcgtccg gtgcgccgcg aggagctggc gtcccagctg gggttccgtc
     8221 accccttcat cttcccttcc ctcctcggga agtcaacgag cctgacggtc atcgacgtcc
     8281 ggtcgcgcga tggaacactc cgctagcctt cccgccgatg gagatccggg aagccacccc
     8341 cgcggaatac gccgaggccg ggcgggtcac cgcggaggcg tatcgcgagt acgtcaggcc
     8401 gggtgaggac gactgggagc gctatctcct ccggatcgcc gacgtccgag ctcgcgccga
     8461 ccagacgacg atcatggtgg ccgtcgagaa cggacgcgtc ctcggatcgc tgaccctcga
     8521 gctcgacggg cgggtgcgcg acggcgatca ggagcaccgc ccgctcgatc cgggcgaggc
     8581 tcacatccgg atgctggggg tggacccggc ggcgaggtcg cggggcgtgg cgcgggcatt
     8641 gatggcgtca tcggaagcgc gagcctatga ggccggaaag accttcatga ccctgcacac
     8701 gactcagcgg atgggagccg cgcaggcgat gtacgagcgc ctcggctacg agcgaatcga
     8761 agatcgcgtg tacccggacg ggttcgtcct gctcacctac gagaagcagc tcgacgtttc
     8821 gaggagtcgg acctgaggga atggtcccgg catgcggcaa cggatagcgg cgttccccgc
     8881 caccgtctgg ccctcgcgga tctggatcgc ggtgttggcg atgaccgtcc tgctggtgtc
     8941 ctgcccgggc ggcagcggcg gctactagtg cgacagcttc agctctcggt gcgaacgacc
     9001 tcgccggaag cgaggatcac gccgtcctcg aactcggcct gcacgaggcc gccacggaac
     9061 tgctgctcgt agagctgcgc gtataggccg ccgtgctcca gcagttcgtc gtgcgtccct
     9121 cgctccacga tgcggccccg atcgagcacg aagatcacgt ccgccgacaa gatcgtcgag
     9181 agccggtggg cgatggcgat cgtcgttcgg cccttcatca acggctccag cgcggattgc
     9241 acgagtcgct cggaggtcgt gtccagcgac gaggtcgcct cgtcgaggat gaggatccgc
     9301 ggatccttca ggacgacgcg ggcgatcgcg agccgctgct tctctcctcc ggacaacttg
     9361 tagccgcgct cgccgacgac ggtgtcgtac ccctcgggga gctccgcgat ccgctcgtgg
     9421 atgttcgccg ccttcgcagc agtgacgagc tcctcgtcgg tcgcgtcggg cttcccgtac
     9481 tggaggttcc ggcggaccgt gtcgtggaac aggtaggtct cctgcgtgac cacgccgatc
     9541 gcctcgccga gcgactcgag cgtgatcttc cggacgtcca gatcgtcgat ctcgaccgca
     9601 ccttcctgga cgtcgtagag ccggggcacg aggtaggtga cggtggtctt gccggcaccc
     9661 gacggtccga cgagcgccgc gagctgtcct ggcctgatgt cgaggtcgac gccgtcgagc
     9721 gtccactccc tcggtacggg gtcgtcgtca cccagcgctg aggcgtcggg tgagaccggg
     9781 atgcttcccc gctcctcggg aggtgggtct tcgtaccgga agcgcacgtc tcggagccgc
     9841 acgcgcccct tcacgtcgtg gatcctcacg gcatccggcg cgtcatggat ctcgtgcggc
     9901 aggtccaggt actcgaagat ccgatcgaag agcgccagcg aggactgcac ctcggtcgag
     9961 acctgcagca tcgagccgat cgggaagaag agccgcgatt ggagcgtggt gaatccgacg
    10021 agcatccccg ccgcgatgac cggccggccc ccgctcgagt tcacccaccc cgcgaccagg
    10081 tagacgaggg cgggcgtgat cgagaagaac gtgcccacca gcgcgaagaa cgaccggccg
    10141 atcatcgtct ggcggagcag gaagccggtc aggcgctcgt tctcgccccg gaagcggtcg
    10201 ctctcgaatc gctgccgacc gaaggccttc gacagcagga tcccgctcac actgagcgtc
    10261 tcctcggtga tcgtcgtcat gtccgcgagg gttcgctggg tgttggtcgc gacctcccgc
    10321 cgggccctgc ccaccttcac cgtgagccag aggaacaacg ggagcaggag cagggaaagg
    10381 atcgtgagcg ggagcgagat caggagcata gcgacgatcg tgctgatgat cgtgacgacg
    10441 ttcgagagca ccgtcgaagc ggtgtcggtg accacggatt gcacgccccc gacgtcgttc
    10501 gccagcctcg actggatctc cccggtcctc gtcgtcgtga agaaccgcag cggcatcgcc
    10561 tggaggtgct ggtagaggcg gttccgcagg tcctgcatca cgttcagacc gacgacgttc
    10621 gtgaggtacg tctggccgat cccgatgatg ctcgtcacga tcgggaccgc gatcatgatc
    10681 ccgacgtaga ggtacagcag cggcaggttc gggcacggcc gcccctgaca gaccccgttc
    10741 ggaccgaaca gggcttcgtc gaagaccagc acgatcatca acgggttgat gacgccgagc
    10801 ccggacgtca cgccgatcat cagcccgacc agaccgacct tgcccttgta cggacggaag
    10861 gtgccgacca cgcgacggac ggtcgcgcgg tcgaccttcc ggtgatcttc gtcgatcgga
    10921 gagacgcgga agaagccgtg acgacccatg gatcgagcat acggggggct gcgggctacg
    10981 cccggcgccc cgtcctgccg agctactcgg cgaagcaggg accgaagatc tcggcgttcg
    11041 tcacgtcgtc gccggggtcg gaccagtagt gatcgacccc cgcaccgccg aacacggcgt
    11101 cattcccgct cgagtcgatc gtcgtgacgc agaagtcgtt cccgtcgccg ccgtacacgc
    11161 gatccgcgcc ggcggagcca ccgtagagcg tgaggtcgcg gccgggaccg ccgtccacga
    11221 cgtcgtcccc gatgccaccg tcaccgtcgt cgatgccgtt cccgccgtgc atccggtcgt
    11281 tcccgccgcg tccgatgatc gtgtccctgc cctgctggcc gtggatgatg tcgtctccgc
    11341 cctctcccgt caggaagtcg tcaccccacc cgcctcgtag gcgatcgtcc ccccggccgc
    11401 cgtagagctt gtcgtcgcca cccatgccgc acaagacgtc gtcaccgggc gttccgtgct
    11461 tcacgtccgg gcccggcgtc cccgtccagg tacacgcggg tggtgcagcc gccgacacga
    11521 gccccagcga gagcgtcgca acgatcaccg caccgagcac cacgatcggt cgaacgatcc
    11581 gcatcttccc ctccccttcc gtccttggtc gtctccgtca ctcgcgtccg accgtaccgg
    11641 cgccggcggg tcccttcaac ggtcacgcgg ggatcgaccg cctcgcggat taggctggtg
    11701 cgccgtgggg accttcgacg aaggccggct gtcgctcgag ctgacgctgg ccgctcggat
    11761 gggactcgct gcgctgctcg ggttcttgat cggccttgaa cgggagtcca ggggcaagag
    11821 cgcgggcgag cgcacgttcg cgatcgtcgc gcttggcgcg gccgcgttct cgagcacagc
    11881 gatcacgatg ttccccgaca ccgccgggca ggcggtggcg ggcgtcgcca cggggatcgg
    11941 cttcctcggc gtcggcatca tctggcgcgc ggaggagggt gagacgcgag ggctcaccac
    12001 ggcggcatcg atcctgtgcg tggccgcgat cggggtcgtg accgggatcg gtctctacgt
    12061 cgccgccatc ctggggacga tactggcgct cttcatcctc gagttcgatc ggatgccggg
    12121 gctccgagga ctgcaccgga gggtcacggg acggccggac gagaacgacg gctagcgagc
    12181 tcgcgagctc ggtggaacac ctcgtcgagc atctggggcg tgagcttccc tgtgaacgtg
    12241 ttctgctggc tcgggtggaa gcagccgagc aggatgaagg gtcccacgct cgcctccatg
    12301 aggtgaccga agcgagggcg gggcctcacc gcgtggccga gcgcggcgag agcccggagc
    12361 gccccgtccc acgcgaacgc cccgagcgcg acgagcacct ggaggtcacg cagaacggcg
    12421 atctcgcgct ccatgtaggg caggcagcgg tcgcgctcct ccggcgtcgg cttgttgccg
    12481 ggcggcgcgc agcggttcac ggcggcgata tagacgccac ggagacggag gccgtcgtcc
    12541 acggagaccg acgccggttg gtccgcgaag ccggtccggt gcagcgacgc gaacaggaag
    12601 tcaccgctcc ggtcgccggt gaagatccgg ccggtccgat tcccgccgtg ggcggcggga
    12661 gccagcccga ggatcaggac agctgcaccc ggatcgcccc agcccgggac cggccgtccc
    12721 caatacgcct ggtctgcgaa tcgagccacc ttctcgcgcg cgacccgctc acgccactcg
    12781 acgagacgcg gacacgctcg gcataccacg acttcgtcgt ggatcctctc gaggctgtcg
    12841 tcgatgctca cacggcggcc ttcgcggcga cccaatcgtc gtagccgccg tcgaagctcc
    12901 gcacctcgcc gtcacggacc tcgaggatcc ggtcgcagag ccgatcgagc aggtaccggt
    12961 cgtgcgagat caggatcgcg gtcccgtcga acgactccac ggcgccctcc agcacctcca
    13021 tgctctcgat gtccaggtgg ttcgtcggct cgtcgaggat caggcagttc gcgccggaga
    13081 gcatcagcag gcagagctcg agccgcgtcc gctccccgcc cgacagggac ccgaccggcg
    13141 tgcgcatctg gtcgtagcgg aagaggaact tcgcgaggag cgccaccgcc tcgccttcgt
    13201 acatcgcctt cgtcttccgg accgatccga cgggcgtgag cgtcgggtcc tggctctgga
    13261 agctctgctc caggtagccg atccggatcg agggaccgat ccaccgctcg cccgtggtcg
    13321 cctcgagctc gccggcgagg atccgcccca gcaccgtttt cccggccccg ttcggtccca
    13381 cgacgccgag ccgctctcgc cggtagacgt cgaacgagac gtcgagcagg accggatcgt
    13441 cgtcgaaggc catcgagacg tcccggagcc gagcgatccg ttgtcccccg cgctcagcgg
    13501 gacggagctc gagcgccatc ttgcgtcgct cgaacacggg acgatccacc ttgtccatct
    13561 gatcgatctg gcgctgcttg ttcctcgctt gcctgatgtg tcgctcgttc ggcacccagg
    13621 acgcccacag ctcgaaccgg gcgatcgcgg cctcgagccg cgcgatctcc ttctgctgcg
    13681 tcacccagac ccgctgctga cgctggagcg cgagctcctt cgcgacggcg taggtggagt
    13741 aggtgcccgg ccatatcgtg accgcgccgc gatcgagttc ggcgatctga ttcacggtgt
    13801 cgtcgaggag gtagcgatcg tgggagacgg tgatgacggc gccggggaac tccgcgacaa
    13861 cgtcccccag gatctcgcgc ttgtcggagt cgaggtgcgt ctcgggctcg tcgagcagga
    13921 gaagacccgg atcgcggatc aggcacgcag cgaacgcggc gagcttgcgc tgcccgcccg
    13981 aaagcgccgt cgtggggagc gagaggtcgt gctcgtcgaa accgagccgg acgagcaggc
    14041 tcctggcttc gcccgcgagc ccgggcccac ccgcctcgac ccagcgatcg agcacagcct
    14101 gctgcttcgt gaggacgcga tccatcttcg cgaggtcggc cgtcaccgct ggccgtgcga
    14161 gctcggcctc gaccgagcgg agctcgtcgt cgagcgcggc gatgtccggc cggccggcga
    14221 gcacccaggc ttcagcggtg cgttcatcac cttcggggtt ctgctcgagg tagctcacgg
    14281 tgatgccgcg tcgccggacc acctcgccct cgttcacgtc ttctgtgcca ccgacgatcc
    14341 ggagcatcgt cgactttccg gcgccgttcg gtccgatgat cccgatccgc gcgccgtcgg
    14401 gcaccatgaa gtcgacccct tggagcacgg gccatgtgcc gtacgacttg tgcaccccga
    14461 cgaggctgac gacggacatc tcagcgacga agcgtacgga accgctcggc cgccgcctcg
    14521 aacgcctcga gcggcgcgag gagctgatcg acggcccaga tcaatccctc gaacccaccg
    14581 agttccggcg gatcctcccg gaggcgccgc gctcggtcta gtgactcacg gatcccggcg
    14641 tcacacgcga gccattcgtc ctcgagctcg tgatgccgcc aggtcggcat caactcctgc
    14701 gcagccgcga gccgttgctc cagctccaaa atcccctcag cgagcggcgt ccccgccgac
    14761 cgcgtcgacg gcacggtgcc ggtcatcgcc agcttcgcct cctcgacccg ctcgaccacg
    14821 gctcggagat ccgcgagggt cgcgtccagc tccggcgaga gccgcttccg gcggaacact
    14881 tagaggacgg tctcgttgcg gaccgggacg atcgcgcgcg ggtcgttcgt ggcgaccgcg
    14941 tccgctccga gctcgaagag acggcgcgcg gtatccggat cgtctgcgat ccatacgccg
    15001 aggagcgcgc cgccatcgtg cacctgatcg gcgaaacccc ggcctgcgtc caagactcgc
    15061 gagacgaacg gcaggatcca tgggtggcct cccgatgtgg ctcgcgcgag cgcttcctcg
    15121 gcggcgacct cgtactcggt gagcaatccg gtcggaacgt ccggtcgcag ggcgcggctg
    15181 tgcgcgatcg aggccgggtt gaagctcgag acgatcacct gacccgcgaa cccggtcgcg
    15241 tcaagggcct cgagcgttgc ttcaacggcc gcctcgcgag ccggcgttaa gcccggctcg
    15301 cccggcaggt tcttgatctc gatgtcggcg gcggcacggc cggacaggca ccgcagggcc
    15361 tcctccaagc ccggcacgcc caacctccgg atctcctcca gcgtcatgtc ggacacgaga
    15421 ccgtgcccgt cggtcgtgcg tgacacgtcg aggtcgtgca tcacgacggc atggccgtcg
    15481 gccgtcatcc ggacgtcgaa ctccaccacg tcggcgccgg catcgatcgc ggcctcgaaa
    15541 gccgcgatcg tgttctcggg ctgctcgacc gacgcgcccc tgtgcgccac gaccatcggc
    15601 cgagccggcc gttcgacgct cacgcctgac tggccgggca cagcg
//
LOCUS       DASUYD010000202         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_575404, whole genome shotgun sequence.
ACCESSION   DASUYD010000202 DASUYD010000000
VERSION     DASUYD010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575404"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VF195_11075"
     CDS             <1..441
                     /locus_tag="VF195_11075"
                     /inference="COORDINATES: protein motif:HMM:NF044818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581399.1"
                     /translation="FAPVTTLGTFSAGKRSSFNGTGFTTSAGDIASGELTLIDVAAGE
                     TSPEGERALAKRAGETVRFVGFVSIRDDTPADEFLLTRYVVTCCVADATIAQVRVVNV
                     TPGRFADEDWVEVTGTIYPLGREVIVDATDITGVARPDRPYMTP"
     gene            complement(1029..>2150)
                     /locus_tag="VF195_11080"
     CDS             complement(1029..>2150)
                     /locus_tag="VF195_11080"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX6581400.1"
                     /translation="QLDTGLYAPLIVEDPSEAGTYDRDIPILLDDWIDGTGETPDEVL
                     ERLLVGGSAMQDMESDASAEMDGMDGMEGMPGGDEGTDGNPHEGTMQEGEELTSGTVR
                     TDGPLGDDTGDVNYPAYLINGRTPSDPAVFEVGAGDRVRLRIINAGSDTPFRVAVGGT
                     PMRVVATDGFAVVPVTVDTLLVGMGERYDVVVSVPGDGAFPLVADAEGKSGRALAVLS
                     AGAGEMPMADIAVPELQGRLLVLDDLVAAESVSLLSGEPDRKYRVGLAGSMATYNWAI
                     TAAEEEGVTLPVHEGERVRLVFENQTAMWHPLHLHGQTFQVVRANGTLGARKDTVIIP
                     ARGQVAVEFIADNPGQWALHCHNIYHAEAGMVITLTYVT"
BASE COUNT          331 a          768 c          705 g          346 t
ORIGIN      
        1 ttcgcgccgg tgaccacgct cgggacgttc tcggcgggca agcggtccag cttcaacggc
       61 acgggattca ctacgtcggc gggtgacatc gcgagcggag agctgaccct gatcgatgtc
      121 gccgccgggg agacgagccc ggaaggcgag cgcgcgctcg cgaagcgagc cggcgaaacg
      181 gtccggttcg tcgggttcgt ttcgatccgc gacgacacgc cggcggacga gttcctgctc
      241 acccgctacg tcgtgacctg ctgcgtggcc gacgccacga tcgcgcaggt ccgcgtcgtg
      301 aacgtcacgc cgggccggtt cgcggacgag gactgggtcg aggtgaccgg cacgatctac
      361 ccactgggtc gcgaggtgat cgtcgacgcg acggacatca caggggtcgc ccggcccgac
      421 cgcccgtaca tgacgcctta actgcagggc aggtcccgat cacgtcaacg cgatggctca
      481 ccccccgaga cgcccttacg gccggcaacg ccgtcgagcg ttcgccgcac tcggaggggg
      541 cggtgacgtc ctcgacggct ccgcaaactc cggcatacga gctggtgatg gcgctcagtc
      601 aggccccagc ccgctcgaag caccgctagg ttccggtaca acgagcgccg tggagcccag
      661 gacggctcgc gacgggggga ccgtcatcgg cgcgtgcagt gggatcccca cgatccgcat
      721 cgctgatccc gccgagacac ggaacccgaa ctcgtaacac gcggacgttg gctgcccgtg
      781 cgcctcaaga tctctctttg catccaactg gcgtccgcgt agacacatcg cttaggtggg
      841 cgagatggca ggtagaggcc ggtcgcgtcg gcgtcggcgt cggtgtcggc gtcggacgtg
      901 ccttcgagcg tcccgatcga ggcaagctcg tggaccggcc gggcgtcatt ggacccacct
      961 gacgcgggct ggttcctgac cttccaggac caactgccga gggccgtacc gtcacccgcc
     1021 gatcacgctc acgtcacgta cgtgagcgtg atcaccatgc ccgcttcggc gtgatagatg
     1081 ttgtggcagt gcagggccca ctggcccggg ttgtcggcga tgaactcgac ggcgacctgc
     1141 ccccgagccg ggatgatcac ggtgtccttc ctcgctccga gggttccgtt cgccctgacg
     1201 acctgaaacg tttggccgtg caggtgaagc ggatgccaca tcgcggtctg gttctcgaac
     1261 accaggcgga cgcgctcacc ctcgtggacg ggcaacgtga ccccctcctc ctcggcggcc
     1321 gtgatcgccc agttgtacgt ggccatgctg ccggcgagcc ccacccggta cttccggtcg
     1381 ggctctccgg acagaaggct caccgactcc gcggccacga ggtcgtcgag aaccagtaga
     1441 cgtccttgca gttcgggaac ggcgatgtcg gccatcggca tctcgccggc gcccgcgctg
     1501 agcaccgcga gggcccggcc gctcttgccc tccgcatccg ccaccaaggg gaacgctcca
     1561 tcgcccggga ccgagaccac cacgtcgtag cgctcgccca tcccgaccag cagcgtgtcc
     1621 acggtcaccg gcacgacggc aaagccgtcc gtcgcgacca ccctcatcgg ggtaccgccg
     1681 accgccacgc ggaacggagt gtccgacccc gcgttgatga tccgcaggcg gacgcggtcg
     1741 ccggcgccca cctcgaagac ggccggatcc gacggcgtgc gtccgttgat caggtatgcg
     1801 gggtagttga catcgcccgt gtcgtcgccg aggggtccgt cggttcgcac ggtcccgctg
     1861 gttagctcct ccccctcctg catcgtcccc tcgtggggat taccgtccgt cccctcgtcg
     1921 ccgccaggca tgccctccat accgtccatc ccgtccatct cggcggacgc gtctgactcc
     1981 atgtcctgca tggcggaccc tccgacgagc aggcgctcga ggacctcgtc cggcgtctcc
     2041 cccgtgccgt cgatccagtc atcgagaagg atcgggatgt cccggtcgta ggtccccgct
     2101 tcagacgggt cctcgacgat gagcggcgca tacaggccgg tgtcgagctg
//
LOCUS       DASUYD010000203         7714 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_135473, whole genome shotgun sequence.
ACCESSION   DASUYD010000203 DASUYD010000000
VERSION     DASUYD010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7714
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135473"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..291)
                     /locus_tag="VF195_11085"
     CDS             complement(<1..291)
                     /locus_tag="VF195_11085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEX6581401.1"
                     /translation="MVAYLIVFLVASGTTFALTPLVRRLAVRFGAIDAPSDRKLHTVP
                     TPTMGGLAMWAGFLVAIAVSRLLPFFEPMNATAVEPLAAVVTCTMMVGLGVID"
     gene            complement(401..1666)
                     /gene="glyA"
                     /locus_tag="VF195_11090"
     CDS             complement(401..1666)
                     /gene="glyA"
                     /locus_tag="VF195_11090"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013288262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX6581402.1"
                     /translation="MREYPADWEALKAEDPEVADAIANELQRERTNLRLIASENYASP
                     AVLAALGSTMTDKYAEGYPGRRYYGGCEFVDVTEQLAIDRAKELFGAEHANVQPHAGA
                     QANLAVFGAFLEPNDPDSKVLGMELAHGGHLTHGSPVNVSGMWFHFIGYRVDEDTEQL
                     DLDEVRDLAKEHRPKIILAGYTAYPRTIDFAAFRTIADEVGALLWVDASHFIGLVAGG
                     AYPSPVPHADVVTFTTHKALRGPRGAMIVCKEEHAKAIDKAVFPMMQGGPLEHVIAAK
                     AVALKEAMQPAFRDYAHRVVRDARALAAGLADEGLRVISGGTDSHLILADVRPVGTDG
                     KTAEAVCDEVGIALNKNQIPYDPSPPSSPSGIRVGTPGPATLGMDEAEMGEVASIIGD
                     VLKHPDDGSVKEKSGQRVRDLMERFPLYP"
     gene            complement(1669..2115)
                     /locus_tag="VF195_11095"
     CDS             complement(1669..2115)
                     /locus_tag="VF195_11095"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HEX6581403.1"
                     /translation="MRIALGADHAGFPLKEDLKSFLAEEGHEVQDHGTDSTEPVDYPA
                     FCAAAARAVVTGAADRAIVLGGSGQGEQIAANKVDGIRAALCHDLFLARLARQHNDAN
                     VLAMGARVVAPSYAREIVRLWLETTFDGGRHETRVKQIGRIERGEL"
     gene            complement(2115..2663)
                     /locus_tag="VF195_11100"
     CDS             complement(2115..2663)
                     /locus_tag="VF195_11100"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphatase family protein"
                     /protein_id="HEX6581404.1"
                     /translation="MSGILVVCTGNICRSPMAEGFLRAALEARLGDGAPVVTSAGTAA
                     WEGSGATAESIRTAAERGVDIEPHVARKLHGAMLEDADLIVCMAADHRSTIVDAMPDL
                     ATKTFTIKGLVRLLEASRPGGSLRARVAAAAAAAARNGSPKRSEDVRDPLGDPIEGYR
                     EIARELHDLADRLAVALAEGEV"
     gene            complement(2660..3298)
                     /locus_tag="VF195_11105"
     CDS             complement(2660..3298)
                     /locus_tag="VF195_11105"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX6581405.1"
                     /translation="MTPLEGPLGEAAAAARRGELIVFPTDTVYGLGTRPDDRAATARV
                     FAAKHRPRDLELPVLVATIEVARSVAEFDGRAERLAGACWPGPLTLVLARGADAAAWD
                     LGGDPATIGVRAPHHPLALALLAETGPLAVTSANRSGHPSVQRCDELHRLFGKDVAVY
                     LCQGDPLEGAASTVLDLAHGPASILREGVLGRDAIAELLPDEAALLDSRPSS"
     gene            complement(3295..4131)
                     /gene="prmC"
                     /locus_tag="VF195_11110"
     CDS             complement(3295..4131)
                     /gene="prmC"
                     /locus_tag="VF195_11110"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEX6581406.1"
                     /translation="MRPAQVVRRAAEYLERHGVESSVPTAERLLSHVLGTDRAGIYAR
                     EELTSQEAKLFGRALCRRCVGEPIQHVIGDQGFRRIVLRVRPGVFVPRPETEILAGAV
                     MDELESVPDPLVVDVATGTGAVALAIKDELPDATMFAIDLSPEAVELARENAHSLGLP
                     MSVLEGDLLEPLPVDLRGRVDAVVCNPPYVAEEARDTLPADVLAEPPLAVFGGIEIYE
                     RLFAQAVGWLAPGGIVAVEIEETAAGIVAKAAAHEGLEGITVRRDLTGRDRVVVARRP
                     HP"
     gene            complement(4133..5221)
                     /gene="prfA"
                     /locus_tag="VF195_11115"
     CDS             complement(4133..5221)
                     /gene="prfA"
                     /locus_tag="VF195_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEX6581407.1"
                     /translation="MDVEDRLREIEARFEQVQAEMSTIEAATDHDRMRTLGRQFAELE
                     QIVRPYRELRQATADAAEARELAAAESDAEMAEAFEEEARAVAERAEALRSRLEELLV
                     PKDPNEGKDLILEIRAGAGGQEAALWAGDLFEMYRHLADRRRWKTDVLASAPAEMGGY
                     KEIVLGIRGRDAYGRLKHESGVHRVQRVPVTESQGRIHTSTATVAVMPEAEEVEVDIR
                     PEDLDIDVYRSSGPGGQSVNTTDSAVRIMHKPTGIKVEVQEERSQLQNREKAMRYLRA
                     RLYQKALEEQQAKEAAARRSMVGTGERSEKIRTYNFPEGRVTDHRIKHTSHQLANVLA
                     GGDELDTFTERLQASERSAQLADPDEKA"
     gene            complement(5267..6058)
                     /locus_tag="VF195_11120"
     CDS             complement(5267..6058)
                     /locus_tag="VF195_11120"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEX6581408.1"
                     /translation="MPTSDLDMPLLPSAEQIRRREFATVRRGYDPQQVRTYLTSIAKQ
                     VGTLERELSQLRLEVGSAAARGEASTATPPSTPAAASSETGDPYDALSKRFAALIEMA
                     DQEAERILENARSESELALDEARTEADRIRVDAQAHAEESRQEGTDLLERARTESSRV
                     LAGLGERRRELIRQLEEMRSKLLTVAEDLVVPIEEAARAEAEDAELVQPIAETRADDD
                     AKAGDADEGETPMDPRYEDLWVHKDPSIEIPDLAAIDLEFDERDE"
     gene            complement(6150..6992)
                     /locus_tag="VF195_11125"
     CDS             complement(6150..6992)
                     /locus_tag="VF195_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX6581409.1"
                     /translation="MMRGAGFWAIAVRKPDASIHIESHEIDSVVLRVPILGKPFLRGI
                     IVLGQSLAIGMRALMISANQSLEEEERLTPRQVVLSVVLALALFVGIFIIGPTTLFAW
                     FENRTGSAGILTLVGEGLFRVALFVAYLFLIGRTKDIHRVFEYHGAEHKTIAAFEHGE
                     ELETEAIDRYPKEHVRCGTNFLIIVMIITIFVFTLFGTPALLWRILSRVVAIPIIAAI
                     SYEALRLGAKHPGSLGMRILMTPGIWLQKITTQQPDASQIEVAVTSFRELLRREAEAT
                     AVER"
     gene            complement(7077..7304)
                     /gene="rpmE"
                     /locus_tag="VF195_11130"
     CDS             complement(7077..7304)
                     /gene="rpmE"
                     /locus_tag="VF195_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015799201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6581410.1"
                     /translation="MKQGIHPDYQIANVHCSCGNTFQTRSTVDEIRAELCSNCHPFYT
                     GKQKFVDAGGRVERFQRRYAKTQAAQQAQQG"
     gene            complement(7393..>7714)
                     /locus_tag="VF195_11135"
     CDS             complement(7393..>7714)
                     /locus_tag="VF195_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014054913.1"
                     /note="An RNA-DNA helicase that actively releases nascent
                     mRNAs from paused transcription complexes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6581411.1"
                     /translation="SALVETGSRMDDGIFEEFKGTGNMELRLDRSLSEKRLFPAINVD
                     ASGTRKEELLMPAEELALVIKLRRVLHALDVGAALELLVDKVRATKSNEQFLKEIAKA
                     PSGG"
BASE COUNT         1145 a         2751 c         2551 g         1267 t
ORIGIN      
        1 atcgatcacg ccgagaccga ccatcatcgt gcacgtgacg accgcagcga gaggctctac
       61 ggcggtcgcg ttcatcggct cgaagaacgg caggagccgg gagacagcga tcgccacgag
      121 gaagccggcc cacatcgcga ggccgcccat cgtcggcgtg gggaccgtgt ggagcttgcg
      181 gtccgacggc gcgtcgatcg cgccgaaccg gacggcgagg cgtcgcacca ggggcgtcag
      241 cgcgaacgtg gtccccgatg cgacaaggaa cacgatcaga tacgcgacca cggtggcctc
      301 agtgtgcggg ggtcctcgcc acccggcaac cggcggacct gctcacggtc agaccgtcag
      361 ggcaacgtcg tcacggccaa gatcgtcacg gccaagatcg tcacgggtag agggggaacc
      421 gctccatcag gtcccggaca cgctgcccgg acttctcctt cacggacccg tcgtccgggt
      481 gcttcaggac gtcgccgatg atcgacgcca cctcgcccat ctctgcctcg tccatcccga
      541 gcgtcgccgg acccggtgtg ccaacgcgga tcccggaggg cgacgagggc gggctcgggt
      601 cgtacgggat ctggttcttg ttcagcgcga tcccgacctc gtcgcagacg gcctcggccg
      661 tcttgccgtc ggtgccgacc gggcggacgt ccgcgaggat caggtgcgag tcggtccctc
      721 cggagatcac acggagacct tcgtcggcca ggccggcggc gagtgcccgc gcatcgcgga
      781 ccacgcgatg cgcgtagtcg cggaacgccg gctgcatcgc ttccttgagc gcgaccgctt
      841 tcgcggcgat cacgtgctcg agcgggccgc cctgcatcat cgggaacacg gccttgtcga
      901 tcgccttcgc gtgctcctcc ttgcagacga tcatcgcccc gcgagggcct ctgagcgcct
      961 tgtgcgtggt gaacgtcacg acgtcggcgt gcggcacggg cgagggatag gcgccgcccg
     1021 caacgaggcc gatgaagtgg gaggcgtcga cccataggag cgcgcccacc tcgtcggcga
     1081 tcgtccggaa cgcggcgaag tcgatcgtcc gcgggtaggc cgtgtagccc gcgaggatga
     1141 tcttcgggcg atgctccttc gcgagatccc gcacctcgtc caagtcgagc tgctcggtgt
     1201 cctcgtcgac gcggtacccg atgaagtgga accacatccc ggagacgttc acgggtgagc
     1261 cgtgcgtgag gtgaccgccg tgggcgagct ccatcccgag gaccttcgag tcgggatcgt
     1321 tcggctcgag gaatgcaccg aagacggcga ggttggcctg ggcgccggcg tgcggctgga
     1381 cgttcgcgtg ttccgcgccg aagagctcct tcgcccggtc gatcgcgagt tgctcggtga
     1441 cgtcgacgaa ctcgcatccg ccgtaatagc gacgacccgg atagccctcg gcgtacttgt
     1501 ccgtcatcgt cgacccgagc gctgccagca ccgcaggact cgcgtagttc tcggaggcga
     1561 tcagccgcag gttcgtccgc tcccgctgca gctcgttcgc gatcgcgtcg gccacctccg
     1621 gatcctcggc cttcagtgcc tcccagtcgg cggggtattc cctcatctct acagctcccc
     1681 tcgttcgatc cgtccgatct gcttcacgcg agtctcgtgg cgtcccccgt cgaaggtcgt
     1741 ctccagccac aggcgaacga tctcgcgagc gtacgagggc gcgaccacac gcgcccccat
     1801 cgcgaggacg ttcgcatcgt tgtgttggcg agcgagacgg gccaggaaga ggtcgtggca
     1861 gagggcagcc cggatgccgt ccaccttgtt ggcggcgatc tgctccccct ggcccgatcc
     1921 tccgagcacg atcgctcggt ccgcagcccc ggtgaccacg gcgcgcgctg ccgccgcaca
     1981 gaaggccggg tagtccaccg gttcggtcga gtcggtcccg tgatcctgga cctcgtgtcc
     2041 ctcctcggcg aggaatgact tcagatcctc tttcaggggg aagcccgcgt gatccgcacc
     2101 gagggcgatc cgcatcagac ctcaccctca gcgagggcca ccgcgagacg atcggccagg
     2161 tcgtggagct cgcgggcgat ctctcgatag ccctcgatcg gatcgccgag tgggtcgcgc
     2221 acgtcctcag atcgcttcgg agacccgttc cgagctgccg ctgccgctgc cgctgcgacg
     2281 cgggcccgga gcgatccgcc agggcgggag gcctccaaca gtcgcacgag gcctttgatc
     2341 gtgaacgtct tggtcgccag gtccggcatc gcgtcgacga tggtcgatcg atgatcggca
     2401 gccatacaga cgatcagatc cgcgtcttcg agcatggccc cgtggagctt ccgggcgacg
     2461 tgcggctcga tgtcgacccc tcgctccgcc gccgtgcgga tcgattccgc cgtcgccccc
     2521 gatccctccc aggccgcggt ccccgccgac gtgaccaccg gcgctccatc gccgagccgc
     2581 gcctcgagcg cggcgcgcag gaacccttcg gccatcggcg agcggcagat gttccccgtg
     2641 cacaccacca ggatcccgct catgacgaag ggcgcgagtc taacaacgcg gcctcgtccg
     2701 gcagaagctc ggcgatcgca tcgcgaccga ggactccctc gcgcaggatg gacgcgggac
     2761 cgtgggcgag gtcgagcacg gtggacgccg ctccctccag cgggtcgccc tggcagaggt
     2821 agacggcgac gtccttcccg aacagccgat ggagctcgtc gcacctctgc acggagggat
     2881 ggccggaacg gttcgcgctg gtgacggcga gcggccccgt ctcggcgagc aacgcgaggg
     2941 cgagcggatg gtgcggggcg cggacgccga tcgtcgccgg gtcccctccc agatcccacg
     3001 ccgcggcgtc ggcaccacgc gcgaggacga gcgtgagcgg gccgggccag caggcgcccg
     3061 cgagccgctc cgcgcgcccg tcgaactcgg cgaccgatcg agccacctcg atcgtcgcga
     3121 ccagcaccgg cagctcgagg tcacgtggcc tgtgcttggc tgcgaagacg cgggcggtgg
     3181 cggcccggtc gtccggacgc gtccccagcc cgtagacggt gtcggtcggg aagacgatca
     3241 actcgccacg gcgggcggcg gcggccgcct cccccaacgg tccctcgaga ggtgtcacgg
     3301 gtgcggccgc cgtgcgacca cgacccggtc acggcccgtg aggtcgcggc gcacggtgat
     3361 cccctcgagc ccctcgtgcg ccgcagcctt cgccacgatt ccggccgcgg tctcttcgat
     3421 ctccaccgca acgatccctc cgggcgcgag ccaaccgacg gcttgggcga agagccgctc
     3481 gtagatctcg atcccaccga acaccgcgag cggcggctcc gccagcacat cggccggaag
     3541 cgtgtcccgg gcttcctcgg ccacgtacgg cggattgcag acgacggcgt ccacccgccc
     3601 ccggagatcg acggggaggg gttcgaggag gtccccctcg aggacggaca ttgggagacc
     3661 cagggagtgc gcgttctcgc gagcgagctc gaccgcctcg ggcgacaggt cgatggcgaa
     3721 catcgtcgcg tcgggcagct cgtccttgat cgccagcgcc acggcgccgg tcccggttgc
     3781 gacgtcgacc accagagggt cgggaaccga ctcgagctcg tccatcaccg ccccggcgag
     3841 gatctcggtc tcgggcctcg gcacgaacac gccgggtcgg acccgcagga cgatgcggcg
     3901 gaacccctgg tcaccgatca cgtgctggat cggctccccc acgcaccggc ggcagagcgc
     3961 gcgaccgaag agcttcgcct cctgcgacgt cagctcttcc cgagcgtaga tccccgctcg
     4021 atcggtcccc aggacgtgcg acaggagccg ctcggcggtg ggaaccgagc tctccacgcc
     4081 gtggcgctcg aggtactcgg ccgctcggcg aaccacctgc gccgggcgca cgtcacgcct
     4141 tctcgtccgg atcggcgagc tgagccgacc gctccgaggc ctgcagccgc tccgtgaagg
     4201 tgtcgagctc gtcgccgccc gccagcacgt tcgcgagttg atgcgaggtg tgcttgatcc
     4261 gatggtcggt gacccttccc tccgggaagt tgtaggtccg gatcttctcg gatcgttctc
     4321 cggttcccac catcgagcgg cgggcagccg cttccttcgc ctgctgctcc tcgagggctt
     4381 tctgatagag acgcgcgcgt aggtagcgca tcgccttctc ccggttctgg agctgggacc
     4441 gctcctcctg gacctcgacc ttgatgcccg tcggcttgtg catgatccgc acggccgagt
     4501 cggtggtgtt cacggactgg ccaccgggtc cgctcgaccg gtagacgtcg atgtccaggt
     4561 cctccggccg gatatcgacc tccacctcct cggcttcggg catcaccgcg actgtggcgg
     4621 tggacgtgtg gatccggccc tgcgactcgg tcacggggac ccgctgcacg cgatgcacac
     4681 ccgactcgtg cttcagccga ccgtaggcgt cgcgtcctcg gatgcccagg acgatctcct
     4741 tgtagccacc catctccgcc ggagccgacg cgagcacgtc cgtcttccag cggcgtcggt
     4801 ccgcgaggtg ccggtacatc tcgaacagat cgccggccca gagcgctgct tcctggccgc
     4861 ccgcgcccgc ccggatctcg aggatcaggt ccttcccctc gttggggtct ttcgggacga
     4921 gcagctcctc gagccgggac cggagcgcct ccgcccgctc cgcgaccgcg cgagcctcct
     4981 cttcgaacgc ctccgccatc tcggcgtcgc tctccgcggc ggcgagctca cgtgcttccg
     5041 cggcatccgc cgtcgcctgc cggagctctc ggtagggccg gacgatctgc tcgagctcgg
     5101 cgaactgccg gcccagagtg cgcatccgat cgtgatcggt cgccgcttcg atcgtggaca
     5161 tctcggcctg aacctgctcg aagcgggcct cgatctccct caggcggtct tcgacgtcca
     5221 tgacgccgac gattctccca ggcgccttcg cggcgccccg ggtccgctat tcgtcgcgct
     5281 cgtcgaactc caggtcgatc gccgcgagat ccgggatctc gatcgagggg tccttgtgaa
     5341 cccagaggtc ctcgtagcgc gggtccatgg gtgtctcgcc ctcatcggcg tcgcccgcct
     5401 tcgcatcgtc gtccgcacgg gtttcggcga tgggctggac gagctcggca tcttccgcct
     5461 cggctctcgc ggcctcctcg atgggtacca ccagatcctc tgcgacggtg agcagcttgg
     5521 agcgcatctc ctccaactgc ctgatgagct cacggcggcg ctcgccgaga cccgccagca
     5581 cccggctcga ttccgttcgg gcccgttcca gcagatccgt cccctcctgc cgggactcct
     5641 cggcatgcgc ctgggcgtcg accctgatcc ggtcggcctc ggtcctcgct tcgtcgagag
     5701 cgagttccga ctcggaccgc gcgttctcga ggatccgctc ggcctcctga tccgccatct
     5761 cgatcagcgc ggcgaagcgc ttcgacaggg cgtcgtacgg atcaccggtc tcggatgacg
     5821 ccgctgcagg tgtcgacgga ggcgtcgccg tcgacgcttc cccgcgtgcg gccgcggaac
     5881 cgacctccag gcggagctgg ctcagctccc gttccagcgt ccccacctgc ttcgcgatcg
     5941 aggtgaggta ggtgcgcacc tgctgcgggt cgtagcctcg acgcaccgtc gcgaactcac
     6001 gtctgcggat ctgttcggcg ctcggcagca gcggcatatc caggtcgctg gtcggcatgg
     6061 ggagaacctc ccgggtcatg gggaccgcat catcttggcg caccgatccc cggatgcaac
     6121 gacctccccg aactgagctg ggagatcggc tatcgctcga ccgcggtcgc ctccgcctcc
     6181 cggcggagca gctcacggaa cgaggtcacg gccacctcga tctgtgaggc atccggctgc
     6241 tgcgtcgtga tcttctgcag ccagatcccc ggcgtcatca ggatccgcat gccgagcgac
     6301 cccgggtgct tcgcaccgag gcggagcgct tcgtacgaga tcgcggcgat gatcgggatg
     6361 gcgaccaccc gcgagaggat ccgccacagc agcgccggcg tgccgaacag ggtgaagacg
     6421 aagatcgtga tgatcatcac gatgatgagg aagttcgtgc cgcagcggac gtgctccttc
     6481 gggtagcggt cgatcgcctc ggtctcgagc tcctcgccgt gctcgaaagc agcgatcgtc
     6541 ttgtgctcgg cgccgtggta ctcgaagacg cgatggatgt ccttcgtcct gccgatgagg
     6601 aagaggtacg cgacgaacaa cgccacgcgg aatagcccct cgccgacgag cgtgaggatg
     6661 cccgccgacc cggtgcggtt ctcgaaccac gcgaagaggg tcgtcgggcc gatgatgaag
     6721 atgcccacga acaacgcgag cgccagcacg accgagagga ccacctgccg aggcgtgagg
     6781 cgctcctctt cctccagcga ctgattcgcg gagatcatca gcgcacgcat gccgatcgcc
     6841 agcgactggc cgagcacgat gatcccccgg aggaacggtt tcccgaggat cggcacgcgg
     6901 agcacgaccg aatcgatctc gtgcgactcg atatggatcg aggcgtcggg cttgcgcacg
     6961 gcgatggccc agaaccctgc cccacgcatc atgacgccct cgacgaccgc ctgccctccg
     7021 tacaggtggg tgcgtggcga tgccggtcgg ggaagttctg cctccgccac gggtggctag
     7081 ccctgctggg cctgttgggc tgcctgtgtc ttcgcgtatc gccgctggaa ccgctcgacc
     7141 cggccgccgg cgtccacgaa cttctgcttg cccgtgtaga agggatggca gttcgagcac
     7201 agctcggccc ggatctcatc gacggtggag cgggtctgga acgtgttgcc gcacgagcag
     7261 tgcacgttcg cgatctgata gtcggggtgg atgccctgct tcatgtcctt ctcctcttcg
     7321 cacctccagg gtagccgacg gcgacgacct ctcggtcccc tggccgatcc agcggcccgg
     7381 tcgacgttcc ggctatcccc cggatggtgc cttcgcgatc tccttgagga actgctcgtt
     7441 ggacttggtg gccctgacct tgtcgaccag gagctccagc gcggcaccca catcgagagc
     7501 gtggagcacg cgccgcagct tgatcaccag ggccagctcc tccgcgggca tgagcagctc
     7561 ttccttgcgc gttcccgagg cgtccacgtt gatcgccggg aacagccgct tctccgacag
     7621 tgaacgatcc agacggagct ccatgttccc ggtgcccttg aactcctcga agatgccgtc
     7681 gtccatccgg gaaccggtct cgaccagcgc gctc
//
LOCUS       DASUYD010000204         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_577300, whole genome shotgun sequence.
ACCESSION   DASUYD010000204 DASUYD010000000
VERSION     DASUYD010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577300"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VF195_11140"
     CDS             complement(<1..690)
                     /locus_tag="VF195_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX6581412.1"
                     /translation="MTPRAADPPRRLFLLDGHSLSYRAFFALPTSLATTSGQVTNAVY
                     GFTSMLIKLLAEEKPDLIAVAFDVGRPTVRLEKYAEYKAGRAETPDEFRQQLGLIVEV
                     LETLRIPVIGIEGHEADDAIATLALRALDRGIEAVIVTADRDFFQLVRPGLTVMFNVK
                     GISDIRRYDVEAVTERFGLPPEKYLDYVALKGDASDNIPGVPGVGEKTASKLVQDFGS
                     VEELLRRTDELK"
     gene            774..1706
                     /gene="efeU"
                     /locus_tag="VF195_11145"
     CDS             774..1706
                     /gene="efeU"
                     /locus_tag="VF195_11145"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HEX6581413.1"
                     /translation="MNPTPWIGFAGAGPGEPELGRDGNDMGAAFVIALREGIEAALIV
                     SILLAYLRQLGRQDRARVVWWGTAAAIAISAAVGTVIFVVGAEFEGTAEEVFEGLVTL
                     TAVVVLTWMIFWMRRQGARIKAELHDKVDTALLTGGAALAAIAFFAVLREGVETALFL
                     FAAAQGTAVESGSVAGQIVGAILGLLLAVALGVLLYRGAVRINLRTFFRLTGGILIVI
                     AAGLFAFSIHELQEAGWFPFLESTAFDVSGALPDDSGVGAILRGLLGYNADPSILEVV
                     AWLGYLLIVGALYLRPVAAPSPKRSAVADGSLNP"
BASE COUNT          308 a          694 c          700 g          357 t
ORIGIN      
        1 cttgagctcg tccgtccgcc gcagcagctc ctccaccgaa ccgaagtcct ggacgagctt
       61 ggacgcggtc ttttccccga cccccgggac ccccgggatg ttgtccgacg catcgccctt
      121 gagcgccacg tagtcgaggt acttctccgg cgggaggccg aaccgttcgg tcaccgcctc
      181 gacgtcgtag cggcggatgt ccgagatgcc tttcacgttg aacatcaccg tgagcccggg
      241 gcgcacgagc tggaagaagt cccggtccgc ggtcacgatc acggcctcga tcccccggtc
      301 gagcgcccgc agagcgaggg tcgcaatcgc gtcgtccgcc tcgtggccct cgatgccgat
      361 cacggggatc ctgagggtct cgagcacctc gacgatcaac ccgagctgct gccggaactc
      421 gtcgggcgtc tcggcacggc ccgccttgta ctcggcgtac ttctccagcc gcaccgtcgg
      481 tctgcccacg tcgaacgcca ccgcgatcag atcgggcttc tcctcggcga gcagcttgat
      541 cagcatcgag gtgaacccgt acacggcgtt cgtcacctgt cctgaggtcg tcgccagcga
      601 cgtgggcagg gcgaagaacg cgcggtacga gaggctgtgg ccgtcgagga ggaacagccg
      661 cctcggcgga tcggcggcgc gaggggtcac gcgcggcatc ctagcgcggt gcagcgaccc
      721 acacgtcctc gggctcgctc ccgacttatc attcgctccc gaatgacctt gacgtgaatc
      781 cgaccccctg gataggattc gcgggcgccg ggccgggcga gcccgagttg gggagagacg
      841 ggaacgacat gggtgccgcc ttcgtcatcg cgctgcgaga ggggatcgaa gccgcgctga
      901 tcgtctcgat cctgctggcg tacctccggc agctcgggcg tcaggaccgc gcgcgggtcg
      961 tctggtgggg tacggcggcg gcgatcgcga tctcggcggc cgtcggtacg gtgatcttcg
     1021 tcgtcggcgc cgagttcgag gggacagccg aggaggtctt cgaagggctc gtcaccctca
     1081 ccgccgtggt cgtcctcacc tggatgatct tctggatgcg acgccagggc gcgcggatca
     1141 aggccgagct ccacgacaag gtcgacaccg ccttgctgac gggcggcgcc gccctcgccg
     1201 cgatcgcgtt cttcgcggtg ctgcgcgagg gcgtggagac cgcactgttc ctgttcgcgg
     1261 ccgctcaggg caccgcggtc gaatccggca gcgtcgcggg ccagatcgtc ggagccatcc
     1321 tcggcttgct cctcgcggtg gcgctcgggg tgctgctgta ccggggcgcg gtccgcatca
     1381 acctccggac gttcttcagg ctcaccggcg ggatcctcat cgtcatcgcg gccggactgt
     1441 tcgcgttctc catccacgaa ctgcaagagg cgggatggtt cccgttcctc gagagcaccg
     1501 cgttcgacgt ctccggagcc ttgccggacg acagcggggt cggcgcgatc ctgcgcggct
     1561 tgctcggata caacgccgat ccatcgatcc tcgaagtggt cgcgtggctc ggctacctgc
     1621 tcatcgtagg tgcgctctat ctacgaccgg tggcagcgcc gtcgccgaaa cgttccgcgg
     1681 tcgcggacgg atcgctgaac ccctgaccag ttgtcgagtg gttcaaccgt ttgtcactct
     1741 ttcttcgttc ggaccgcgcg cgccgctacc tgcaacgtca cccggatcgc cttgcagccc
     1801 aatcgttccc atcgcgttga caggtacgag gcacgtcgct acatttcgct ccggtcccga
     1861 gcggccgcag ggaccgggag gcggaaagcg cctccgcaga gggggagcaa cgaaggggcg
     1921 cgagtcccgg agccgcttca ccgatacgga ggaggcgacg gttcccggga gtccgggtcg
     1981 cttggggcca ccttttgacg ggtcgaggaa ccgagtagac aacgctcgcg tatggggaag
     2041 gcgtgggcgg atccaaagg
//
LOCUS       DASUYD010000205         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_138765, whole genome shotgun sequence.
ACCESSION   DASUYD010000205 DASUYD010000000
VERSION     DASUYD010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138765"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VF195_11150"
     CDS             complement(<1..566)
                     /locus_tag="VF195_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006943383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX6581414.1"
                     /translation="MTSTATTLPEGIRAVIFDMDGVITDTTSVHAEAWKRLFDEYLRE
                     RADRTGEPFVPFDADEDYRRYVDGKGRFDGVRSFLASRGIELPEGEPNDPPDRRTICG
                     LANLKNEMFLEHIRRHGVQPYPSTVALVRELQTRGIGTAAISASRNMTEVLDAAGIGN
                     LFPVTVDGTDLDELGLPGKPDPAMFVEAA"
     gene            863..1060
                     /locus_tag="VF195_11155"
     CDS             863..1060
                     /locus_tag="VF195_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011604017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0010498 - proteasomal protein catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019941 - modification-dependent protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein Pup"
                     /protein_id="HEX6581415.1"
                     /translation="MERVHEQPKSRHRHRADVVEDGSIDNGVNATDLQRELDDLLDEV
                     DAVLEENAEEFVKAYVQKGGE"
     gene            complement(1087..2325)
                     /locus_tag="VF195_11160"
     CDS             complement(1087..2325)
                     /locus_tag="VF195_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581416.1"
                     /translation="MLLQTSCTSEPERRDLPDVQIDIPEEASTDTASEGPAPTVPSDV
                     ADLPGRLAVLDAAGNLVTSNPDGSDEVVLAEIETGGSQVLQPTWSPDGRRVAWVDVEV
                     TEAGGLSSVIATAGGDGTEPSEARTAVVPFYLSWDPTSSRIAYLGSPSEQDIELGILE
                     VAGRSSGTPLDAGQPFYLSWAPGGDQLLVHIGEDRLELLDLDGSLTTVADRPGTFSAP
                     VWTADGRTFVYASVGAERQRLVVRGTGAENERPLVSFDELISFVVSPDGERIAFQVIE
                     EQGVLPLSVVDVDTGKTVEITDAAIAAFFWSPDGERLLYLDPDPEDEQFWFRWGVWDG
                     RSSFLTPRFVPSQLIAGAYLRFFEQYAQSMNLWSPDGSAFAYPGANEDGVEGIWIQSA
                     RGDRVPVLVADGDFVAWSPA"
     gene            2553..3266
                     /locus_tag="VF195_11165"
     CDS             2553..3266
                     /locus_tag="VF195_11165"
                     /inference="COORDINATES: protein motif:HMM:NF025580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4328 domain-containing protein"
                     /protein_id="HEX6581417.1"
                     /translation="MSIRPTERIAGALVTLLGTQIALLVAEAFALLYRIRLLGRVQTG
                     GSITLTEADRADVLVIASVWVWLLVFVGTGIVWCVWQYRAQRNAIELASGGLKFTPGW
                     AVGWWFVPLANLVKPFETVRELWKASHGVAWRSLNTWPVIGWWWALWLAGSALEWGIR
                     RSDTPTDISGFITQDLWRLASLGFELGSAVLAIAIVRSIVGLQEGVAVSSIPPMPGVV
                     TPASADGQAVLGLPPPPSG"
     gene            complement(3282..>3458)
                     /locus_tag="VF195_11170"
     CDS             complement(3282..>3458)
                     /locus_tag="VF195_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011726109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX6581418.1"
                     /translation="SGHASVGDQALARSVLGVPEDRECAILVALGYPADRPLEPIAHP
                     NRRSLDDVVHRERW"
BASE COUNT          539 a         1163 c         1210 g          546 t
ORIGIN      
        1 gcggcctcga cgaacatcgc cggatccggt ttgccgggca ggccgagctc atcgaggtcg
       61 gtgccgtcga ccgtgaccgg gaagaggttt ccgattcccg ccgcgtccag gacctcggtc
      121 atgttccggc tcgcggagat cgccgccgtc ccgatgccgc gtgtctgcag ctcgcggacc
      181 agggccacgg tcgacgggta cggttgcacg ccgtgccggc ggatgtgctc gaggaacatc
      241 tcgttcttca gattcgccag cccgcagatg gttcgccgat ccggagggtc gttcggctcg
      301 ccctcgggca gctcgatgcc tcgcgaagcg aggaagctcc gcacgccgtc gaaccgaccc
      361 ttgccgtcga cgtaccggcg gtaatcctcg tcggcgtcga acggcacgaa cggttcgccc
      421 gtgcgatcgg cgcgctcgcg caggtactcg tcgaacagcc gcttccacgc ctcggcgtgc
      481 acggacgtgg tgtcggtgat gacgccgtcc atgtcgaaga tcacggcgcg gatcccctcg
      541 ggcagggtgg tcgcggtcga cgtcatgacg gcgaccagtc tggcgcacgg ttcggccagg
      601 cgtcccccta gagttccgaa agatcccaag gaccgggcgc gggatggtga cgccgccctt
      661 catgactcga tcctcctctc gatcgctcgg catcgaccgc cgatccaaac gggtgtcaag
      721 tcggccgtgc gggctaacga gattgtggtg ctcgaggcct acgcctttcg tccacgaagg
      781 gtccgcatcc ggtgagtgac tgggagtgtg ccatgagtca acactcatcg ctgccgatgt
      841 ggttgggaag aggacggagc gcatggagcg agttcacgaa cagccgaaga gccgtcaccg
      901 ccatagggcg gacgtcgtcg aggacggatc gatcgataac ggcgtgaacg ccacggacct
      961 ccagcgcgag ctggacgatc tcctcgacga ggtcgacgcc gtcctcgaag agaacgcgga
     1021 ggaattcgtc aaggcgtacg tccagaaggg cggcgagtag gactccgcgc cgagcggatc
     1081 aagccctcag gcaggcgacc aggccacgaa gtcgccgtcg gcgacgagga ccggtacgcg
     1141 gtctccccga gcggactgga tccagatccc ctccacgccg tcctcgttcg cccccgggta
     1201 cgcgaacgca ctgccgtccg gcgaccacag gttcatgctc tgcgcgtact gctcgaagaa
     1261 ccgcaggtac gctcccgcga tcagctggct cgggacgaag cgaggcgtga ggaacgacga
     1321 cctcccgtcc cacacgcccc agcggaacca gaactgttcg tcctccggat ccgggtccag
     1381 gtacagcagg cgctcgccgt cggggctcca gaagaacgcc gcgatcgcgg catcggtgat
     1441 ctcgacggtc ttgccggtgt cgacgtcgac gaccgagagc gggagcacgc cctgctcctc
     1501 gatcacttgg aacgcgatcc gctcgccgtc cgggctcacg acgaaggaga tcagctcgtc
     1561 gaacgagacg agcggccgtt cgttctcggc cccggtgccc cgcacgacca gtcgctgacg
     1621 ctccgcaccg accgacgcgt agacgaaggt ccggccgtcg gcggtccaca cgggcgcgga
     1681 gaaggtgccc ggtcggtcgg cgaccgtggt cagcgatcca tccaggtcga ggagctcgag
     1741 ccgatcctcg ccgatgtgca cgagcagctg atcgccgccc ggcgcccacg agaggtagaa
     1801 gggttgtccg gcgtcgagcg gcgtgcccga gctccgaccg gcgacctcca ggatccccag
     1861 ctcgatatcc tgctcgctcg gcgacccgag gtaggcgatc cgggacgagg tggggtccca
     1921 ggagagatag aacgggacca ccgcggtcct cgcctcggag ggctcggtgc cgtccccgcc
     1981 ggcggtggcg atgacggacg agaggccgcc cgcctcggtc acctcgacgt ccacccacgc
     2041 gacgcgacga ccatccggcg accacgtcgg ttggaggacc tggctcccac cggtctcgat
     2101 ctcggcgagg acgacctcgt ccgaaccgtc cgggttggac gtgaccaggt tcccggcggc
     2161 gtccaggacg gcgaggcgac ccgggaggtc cgccacatcc gagggaaccg tcggcgcggg
     2221 gccctcagag gcggtatcgg tcgaggcctc ctcggggatg tcgatctgga cgtcgggcag
     2281 gtcacgccgc tccggctcgg acgtgcacga cgtctggagg agcaccagcg ctgccaggca
     2341 caggaagagg cggctcccga ggtggccgcg ttcgctacgt cgaggatccg gtggcacagt
     2401 cccaagtgtc gaccgcatcc agtgcttggg caatgccgag cgaacgttcg gtccagcggg
     2461 tccggctgtt ttccgtgcgg ttcaggcagg ggttccgtct cccgatacgt agagggttca
     2521 ggcgtggtcg gacccgatct ccgggaggtc ccatgtccat tcgaccgacg gaacggatcg
     2581 cgggcgccct cgtcaccctg ctcggcacac agatcgccct tctcgtggcc gaggcgttcg
     2641 ccctgctcta tcggatacgc ctcctcggcc gtgtgcagac cggcggttcg atcaccctga
     2701 ccgaggccga tcgggccgac gtcctggtga tcgcgtccgt gtgggtctgg ctcctggtgt
     2761 tcgtcgggac gggcatcgtg tggtgcgtct ggcagtaccg ggcgcagcgg aacgcgatcg
     2821 aactcgcgag cgggggcctc aagttcacac cgggatgggc ggtggggtgg tggttcgtcc
     2881 cgctcgcgaa cctcgtcaag ccgttcgaga cggtccgcga gctgtggaag gcgagccacg
     2941 gtgtcgcctg gcggagcctc aacacttggc ctgtgatcgg gtggtggtgg gcactctggc
     3001 tcgccgggag cgctctcgag tgggggatcc gccgctccga caccccaacc gacatctccg
     3061 gcttcatcac ccaagacctg tggaggctcg catcgctcgg cttcgagttg ggttcggccg
     3121 tcctcgcgat cgcgatcgtt cgttccatcg tagggttgca ggaaggcgtg gccgtgtcat
     3181 cgatcccgcc gatgccgggc gtcgtcacgc ccgcgtccgc ggacggacag gctgtgctcg
     3241 gcctgccgcc gccgccgagc gggtagcggt cgcgggccca gctaccaacg ttcgcggtgg
     3301 acgacgtcgt cgagggaccg ccggttcgga tgtgcgatcg gctcgagcgg acggtcggcc
     3361 ggatagccga gcgcgacgag gatcgcgcac tcccgatcct cgggcacgcc gaggacgctg
     3421 cgcgcgaggg cctggtcgcc caccgaggcg tgcccgct
//
LOCUS       DASUYD010000206         4532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1243056, whole genome shotgun sequence.
ACCESSION   DASUYD010000206 DASUYD010000000
VERSION     DASUYD010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243056"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VF195_11175"
     CDS             <1..487
                     /locus_tag="VF195_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581419.1"
                     /translation="GDLVKRYGQIAFRVAWLVTRDRGEAEDAAQEAFVKAYYALPRFR
                     PDSPFRPWLLKIVANEARNRARSTRRRQGLALRAAAVSEGDAAPSPETAALAVADREA
                     LVAALNALSPPDREVIGYRFLLGLSESETADVLGVRQGTAKSRLSRAMARLRAVLEEP
                     A"
     gene            484..1257
                     /locus_tag="VF195_11180"
     CDS             484..1257
                     /locus_tag="VF195_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581420.1"
                     /translation="MSERLRSMSDDDLGAALSSLDIEWPATPALHAAVLARATSERPP
                     IVRLPLSRPRRIMLIAAATVLLLAGAAVAAKIVIDLGGVVVEVTPSRPGILPTPSIAP
                     TGEPVTLEEAAVLLGREVPVPAGLGRPDRVWADEVVTEKGEVARVTLAWRARQGLPEI
                     SETRYGAVLMVFEGDANLASKDLYEDTGVLRFESVDGVDYYWTAGIHLLELLTSEGVA
                     YVRVEGNVLLWRDGSDTMRLETSLPMTEALRVAGSVGTP"
     gene            1303..1890
                     /locus_tag="VF195_11185"
     CDS             1303..1890
                     /locus_tag="VF195_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX6581421.1"
                     /translation="MRRGMLLCTIVVLAAVVGMPGVASAGGGCHGDVTENDATGQEDP
                     TIQMVDACFTATVTTVDPGTPVTFVNMDEFVHNVGGNQWGHFDDLHGGDTFRVSFDEA
                     GTYPFACSYHPGMTGAIVVGDGKGAGSGAGIAVEPFEAPGPETVTRVVTTSGGGWTGS
                     VAVAAIIGGLLGAAVTVGVTRSVSRKVPARVREPI"
     gene            complement(1887..2987)
                     /locus_tag="VF195_11190"
     CDS             complement(1887..2987)
                     /locus_tag="VF195_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6581422.1"
                     /translation="MENTDSAVAEFDGWLKELMVGQGSDLHVKVGSPPMIRLPGGLER
                     MSRDPITPVETAAIAESIIPADRKETFDETGEIDFAYSLRDVGRFRANVFKQRGSISM
                     VLRRLRFGGPTFEEMGLPDTVRRLAEEHRGLILVTGPTGSGKTTTLASMIGYINETKP
                     VHIVTIEDPIEVLHRDAVASINQREIGNDTQDFLSALRAALRQDPDVILIGEMRDTET
                     VRAAIQAAETGHLVLSTLHTVDATETVNRVIDFFPPYQQKQVRLALAGTLRGIICQRL
                     VTTVDGGRTPTLEILVNNGRIAERIVDPERTSEIEEVVADGAFYGMMTFDQSLLVLLQ
                     EGKISTDAAFEAVSSRHDFELAMQQAGLSLPV"
     gene            complement(3107..3781)
                     /locus_tag="VF195_11195"
     CDS             complement(3107..3781)
                     /locus_tag="VF195_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX6581423.1"
                     /translation="MNEPAPIEIHKALADDTRYRLYRYLRLSGRPVPVRELATRLSLH
                     ANTIRPHLRRLADAGLVASETRRGPSAVGRPQTVYTAVATDDREGSDYRLFADILATV
                     TTGSRARAGAHTLAREWGDYLVTRHAPAPGARRPGLNLAGLREALAEAGFDPRFRRKG
                     PRTVEIALRGCPFRDLLEEHRELVCSVHRGLLEGMLEGSRPRLHLKAFEPLAERSSVC
                     RVVAGD"
     gene            3904..>4532
                     /locus_tag="VF195_11200"
     CDS             3904..>4532
                     /locus_tag="VF195_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010047745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX6581424.1"
                     /translation="MATEAQVREALKGVRDPEIGRPIDEIGMLRSVEVDAGVVRVHVL
                     LTIEGCPLKDRITSDVTAAVQPLEGVQRVEVELTPMSSEEREKLVSGLRGGQPAAPET
                     TIAFPPQTAIIAIASGKGGVGKSSVTVNLAAALAKTGRSVGVLDADVWGFSVPRMLGA
                     VGKPVGFNDMILPLEAHGVKVISMGHFVPEETPVIWRGPMLHKAIQQFLG"
BASE COUNT          681 a         1520 c         1604 g          727 t
ORIGIN      
        1 cggagacctg gtgaagcggt acgggcagat cgccttccgt gtcgcctggc tcgtcacgcg
       61 cgatcgcggt gaggcggagg acgcagccca ggaggcgttc gtgaaggcct attacgcgct
      121 cccccggttc cggccggact ccccgttccg gccgtggctg ctgaagatcg tcgccaacga
      181 ggcgcggaac cgcgcgcgat ccacgcgccg tcgccagggc ctcgccctgc gcgcggccgc
      241 ggtctccgaa ggggacgcgg ccccgtcccc ggagaccgcg gcgctggcgg tcgccgaccg
      301 ggaggcgctc gtcgctgcgc tgaacgccct ctcgcctccg gaccgggaag tcatcggtta
      361 ccggttcctc ctcggcctgt cggagagcga gacagccgac gtgctcggcg tgcgccaggg
      421 gacggccaag tcccgcctct cgagggcgat ggctcgcctg cgcgcggtgt tggaggagcc
      481 ggcatgagcg aacggctccg ttcgatgagc gacgacgacc tcggtgccgc tctgtcctcg
      541 ctggacatcg agtggccggc cacaccggcc ctgcatgccg cggtcttggc tcgcgccacg
      601 tcggagcggc cacccatcgt gcgtctcccc ctgtcccgtc ccagacggat catgctcatc
      661 gctgcggcga ccgtgctgct gctcgccgga gccgcggtcg cggcgaagat cgtcatcgat
      721 ctcggcggtg tggtcgtgga ggtcacgccg agtcgtccgg ggatcctgcc gacgccctcg
      781 atcgctccga ccggcgagcc ggtgaccctc gaggaggccg ccgtcttgct cgggcgggag
      841 gtccccgtgc ccgcgggtct cggccgaccc gaccgcgtct gggcggacga ggtcgtcacg
      901 gagaagggag aggtcgcccg ggtcacgctc gcatggcgag cccgccaggg cctcccggag
      961 atctcggaga ctcgatacgg agccgtcctg atggtgttcg agggcgacgc caacctggcc
     1021 tcgaaggacc tgtacgagga tacgggtgtc ctgcgattcg agagcgtcga cggggtcgac
     1081 tactactgga ccgcgggcat ccacctcctc gagctgctca cgagcgaagg tgtcgcctac
     1141 gtgcgcgtcg agggcaacgt gctcctgtgg cgggacggct ccgacacgat gcggttggag
     1201 acctccctgc cgatgaccga ggccctgcgg gtcgccgggt cggtgggaac tccctgatcc
     1261 cttccggtgt agcagaggca tcggacgtca ggagggatcg acatgaggcg ggggatgctg
     1321 ctctgcacga tcgtggttct cgcggccgtc gtcgggatgc ccggcgtggc gtcggcgggc
     1381 ggtggctgcc atggggacgt cacggagaac gacgcaaccg gccaggagga cccgaccatc
     1441 cagatggtcg acgcatgctt cacggcgacg gtcacgaccg tggatccggg aacgcctgtg
     1501 accttcgtga acatggacga gttcgtccat aacgtcggag gcaaccagtg gggccacttc
     1561 gacgacctcc acggtgggga taccttcagg gtgtccttcg acgaggccgg cacgtacccg
     1621 ttcgcgtgca gctatcaccc cggcatgacc ggcgcgatcg tcgtcggcga cggcaagggt
     1681 gccggcagcg gcgcgggcat cgcggtcgag ccgttcgagg caccgggtcc cgagaccgtg
     1741 acgcgggtgg tcactacgag cgggggcggt tggacaggtt ccgtcgcggt cgccgcgatc
     1801 atcggcgggc tgctcggagc cgcggtcacc gtgggggtga cccgttccgt ttcgaggaag
     1861 gtgcccgccc gagtgcgtga accgatctag acgggcaagc tcagccccgc ctgctgcatc
     1921 gcgagctcga agtcgtgccg gctcgatacg gcctcgaacg ctgcatccgt gctgatcttc
     1981 ccctcctgca gcagcaccaa cagcgattgg tcgaacgtca tcatgccgta gaaggcgccg
     2041 tcggcgacga cctcctcgat ctcgctcgtg cgctccgggt cgacgatccg ctcggcgatc
     2101 cgtccgttgt tcacgaggat ctcgagggtc ggggttcggc cgccgtcgac cgtggtgacg
     2161 aggcgctggc agatgatgcc gcggagcgtg ccggcgagcg ccagccgcac ctgcttctgt
     2221 tggtacggtg ggaagaagtc gatcacccgg ttgacggtct ccgtggcatc gacggtatgg
     2281 agcgtggaca ggaccaggtg tcccgtctcg gccgcctgga tcgcagcccg caccgtctcc
     2341 gtatcccgca tctcgccgat caggatgacg tcgggatcct gccgcagcgc cgcgcgcaag
     2401 gcggagagga agtcctgcgt atcgttgccg atctcacgct ggttgatcga ggccaccgcg
     2461 tcgcggtgca gcacctcgat cgggtcctcg atcgtgacga tgtgcaccgg cttggtctcg
     2521 ttgatgtagc cgatcatgga agcgagggtc gtggtcttac cggatcccgt ggggccggtg
     2581 acgaggatca gccctcgatg ctcctcggcg agacgccgga ccgtgtcagg caaccccatc
     2641 tcctcgaagg tgggtccacc gaaccggaga cggcggagca ccatcgagat agagcctcgt
     2701 tgcttgaaga cgttggcacg gaaccggccg acgtctcgca gggagtacgc gaagtcgatc
     2761 tcccccgtct cgtcgaacgt ctccttccgg tccgcgggga tgatgctctc ggcgatcgcg
     2821 gcggtctcca ccggcgtgat cgggtcgcga gacatcctct ccaacccacc gggcagcctg
     2881 atcatcggcg gcgagccgac cttgacgtgc aggtccgagc cctgcccgac catgagctcc
     2941 ttgagccagc cgtcgaattc cgcaacggcg gaatcagtgt tctccactgc gccacacctc
     3001 gtctcgagcg atggccgcgc acgcgtaccg tccgcgctcg gttcctccct ctcccatcgg
     3061 cccgtcggcc gaccaacttc agcgcgaccc gtgagttgcc gtgcgctcag tcgccggcga
     3121 ccactcggca gaccgagctg cgctcggcga ggggttcgaa cgccttcaga tgaaggcggg
     3181 ggcgcgatcc ttccaacatc ccttccagca gtcctctgtg caccgagcac accagctccc
     3241 tgtgctcctc gagcaggtca cggaacggac agcctcgcag ggcgatctcc acggtgcggg
     3301 gccccttgcg ccggaagcgc ggatcgaatc ccgcttcggc cagtgcctct ctgagccccg
     3361 ccaggttcag ccccggacgg cgcgccccgg gcgccggtgc gtgccgggtg acgagatagt
     3421 cgccccactc tcgagcgagt gtgtgggcac ccgcccgagc gcgcgacccg gtcgtgaccg
     3481 tcgccaggat gtccgcgaac aaacggtagt cgctcccctc ccggtcgtcc gtggcgactg
     3541 cggtgtagac ggtctgtggg cgcccgacgg cagaagggcc tcgccgcgtc tccgacgcca
     3601 ccaggccggc gtccgcgagc cgacgcaggt gggggcggat cgtgttggcg tgcaggctca
     3661 accgggtggc gagctcgcgg acggggaccg ggcggccgga caagcgcagg taccgataga
     3721 gccggtagcg ggtgtcgtcc gcgagggcct tgtggatctc gatcggggcc ggttcgttca
     3781 tctccgcatc tcgtacgatg ggctttgcac ggttcgcacc gtgtcaacca gagcttagac
     3841 cccagatccg aacgagaccc aggaacgaag ccaggcaccc gccactcgat gggaggagag
     3901 cccgtggcca cggaggcgca ggttcgggag gccctgaagg gcgtccggga tcccgagatc
     3961 ggccgcccga tcgacgagat cgggatgttg cgatccgtcg aggtggacgc cggcgtggtc
     4021 cgcgtccacg tcctgctgac catcgaagga tgtccgctca aggatcggat cacgagcgac
     4081 gtgacggcgg ccgtccagcc gctcgaggga gtgcagcgcg tggaggtgga actcacgccg
     4141 atgagctcgg aagaacggga gaagctcgtc tcgggcctcc gcggggggca acccgccgcg
     4201 ccggagacca cgatcgcgtt cccgccgcag acggcgatca tcgcgatcgc gtcgggcaag
     4261 ggcggagtcg gcaagtccag cgtcactgtg aacctggccg ccgcgctcgc gaagaccggt
     4321 cgctcggtcg gggtgctgga tgccgacgtg tggggcttca gcgtcccgcg gatgctgggc
     4381 gcggtcggca aacccgtcgg attcaacgac atgatcctgc cgctggaggc ccacggggtg
     4441 aaggtgatct cgatgggcca tttcgtgccc gaggagacgc cggtgatctg gcgcggcccg
     4501 atgttgcaca aggcgatcca gcagttcctc gg
//
LOCUS       DASUYD010000207        10922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_139785, whole genome shotgun sequence.
ACCESSION   DASUYD010000207 DASUYD010000000
VERSION     DASUYD010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139785"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..569)
                     /locus_tag="VF195_11205"
     CDS             complement(222..569)
                     /locus_tag="VF195_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581425.1"
                     /translation="MSTKANVDFDGLMRHLMVVGFVALTAWVAASATGMALGVLQISP
                     SATLRFLLAVLVLVFARRTYWEIQEWRWRRLPPDERYGFASPIWEHPRSAELETSRNG
                     ADRGAEGEPEPRR"
     gene            714..800
                     /locus_tag="VF195_11210"
     tRNA            714..800
                     /locus_tag="VF195_11210"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Leu,seq:cag)
     gene            981..1502
                     /locus_tag="VF195_11215"
     CDS             981..1502
                     /locus_tag="VF195_11215"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HEX6581426.1"
                     /translation="MTLSPFIAALVLSPSTLTLVALVAIGLAIVAVLYSIANAGKAGG
                     SVGGRVKLDETLRGILQGQGEQMQRLERAIRALHATGKKQHAEIEGSVRNVALLRYDA
                     FEDVGGRLSFSCALLDDHGDGVVLTSINGRQETRVYAKQVTQGTSSHNLSLEEEEAIR
                     RAMGGRQATVETG"
     gene            1574..2026
                     /locus_tag="VF195_11220"
     CDS             1574..2026
                     /locus_tag="VF195_11220"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6581427.1"
                     /translation="MAAENGEVTIVGAGARLEGNVVSAGNLRIDGQVKGQINAEGDVA
                     LSPQSQVEADIRAQNVSIAGRFKGSIQVNGKAHLARGGRIDGNITSKTLVVEEGGVFH
                     GQSIMDGSAPTEAARSQGATPAKGPENEPENAPENAQEKRDEPEVKVP"
     gene            2027..3301
                     /gene="serS"
                     /locus_tag="VF195_11225"
     CDS             2027..3301
                     /gene="serS"
                     /locus_tag="VF195_11225"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX6581428.1"
                     /translation="MLDIRRIREDPDPFRAALARRNLGDAVDRLLEADERRRSLTSRV
                     EELRATQNRASKAIGAAKGDEKQQLIDEVAKVSAELKDLDPELAAAEEGLRSLLAEIP
                     NLPHESAPDGFTDEDAVEVRRNHEEPPVLGFEPRDHAELGAMLGVLDTERGARTSGSR
                     FVYLMGDLVFVQFALMRHAMDVVVDKGFSPMIPPVLVREEAMYGTGFLPTDAVNIYVT
                     REDELYLTGTSEVALAAFHLGEILDEADLPIRYAGYSSCFRREAGSYGKDLGGMFRVH
                     QFDKVEMFAFCTPETSWDEHERILAIEEEILGNLEIPYRVVNVAAGDLGGSAAKKYDI
                     EGWLPAQQRYRELTSCSNTTDYQARRMRTRLRRAGGDVEVLHTLNGTATAIGRTLIAI
                     MENHQRPDGSVEIPAKLHPYLPERARVLAPRS"
     gene            complement(3326..5506)
                     /locus_tag="VF195_11230"
     CDS             complement(3326..5506)
                     /locus_tag="VF195_11230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6581429.1"
                     /translation="MPGHPHSRAPEPLDPFASGSRVRELVEQLPVVVYVDTDDQRPST
                     TYISPNVQQLLGFPPQRFLEDPGLWIRCMHPDDRERIQAIRDEVWSSGARYRSEYRMF
                     RRDGEEVWLRDSSVLVLSDEGQRLAWQGVIEDITTERRAEQEIRASEARYRALVERVP
                     AVVYEMGPDDERQTLFVSPHVEQVLGYSREEWLDQPDIWIELLHADDREVVLAKHDRH
                     NETGEPWDLEYRLIASDGRVVWVHDRASLIRGLERGAAAWHGVMIDITAEHEATEMLL
                     LLKEDLERRVAERTSELREANELMSLEIGERRRMELELRDVEERYRRLVENMPGIAYV
                     WEVRPGAEARTFGYVSPRIEDILGFSPEEWHASARVHPHDQARVSEAVDRSVRTGEAF
                     VMEYRYLAKDGSVVWVLDHASLISRSDTGDPASFQGMMLDVTSRMEAESKAAAAEDRY
                     RTLTERGPVVAYSYELSYVDGADPSLHVSHVSPQAADLVQYPIGHWLHEPEVWFGMIH
                     PDDRERVAQMTAHNWRTGEPWMLRYRMIRSDGTLIWLLDAGRLLDRDTRGRPWTFQGI
                     LLDVTTDEEARAQVEATAGAQREALEGALAIPWSETIHPDTGVERYTYIGSQAFDILG
                     YTPEELMDEPKHFPRMVHPDDRPRVHQSIQRSDETGAWDDTYRILRRDGEIRWLHSFG
                     RRTSASGVIPETWHGVAIDVTAYRAEAGADEGVAAGDAVVDPAQ"
     gene            5576..5821
                     /locus_tag="VF195_11235"
     CDS             5576..5821
                     /locus_tag="VF195_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581430.1"
                     /translation="MGTACSPGAYIGAAGLALAGIALIAYFVGGQGWRTRTKVILLGG
                     AGLLSAVLIVTGVELKLREEHDVVKSATGTEVVCHHV"
     gene            5887..5973
                     /locus_tag="VF195_11240"
     tRNA            5887..5973
                     /locus_tag="VF195_11240"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5921..5923,aa:Ser,seq:tga)
     gene            complement(6066..7802)
                     /locus_tag="VF195_11245"
     CDS             complement(6066..7802)
                     /locus_tag="VF195_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581431.1"
                     /translation="MDASIEATTDPTAATADTAIEDTDATGTTVAAPDALSGIVTDAV
                     SDGTSQDPVGGTDAASETLEAPQEAPQAVDVTVTDVTSATTDEDAATNAISATPDIVT
                     ETTSDAVAVSADATADATTGTISAEAGSDEQPSLDATAAASETATDVAAMIAHTSTEA
                     TDAVVVPEATEGTGAIPDATSEVATAVGDATVEAASMVETTSDTAGMTTDVTAGATMS
                     AEAITQTAVREATAITVESSAVIGGATDATWATFETVATEASATVSNVASLEAVTGTT
                     STIADAATAVSDTSPEIITGVEEVLAFSDPATQIIVDSTTTTSEATTDVTALLGQTAT
                     EAQVTLAETPADTNPETAVTVVDAMTEGSGTADPVVGMASEITSETVGAGPDGTSNPV
                     ATLSEATSAGVVADGIDDGLADPMTVLNEATETVMSDLYPASLGSTDQASEGAISGPM
                     TFGRVWGIQGGSSPIGTERLLSVNRGQIEGERLQGNEPVATCDGASSLSCAVTGAADG
                     IDSLVESVASIIRILALTGLTLLPWVAAAGVLAFLGGLALTASRRRRATSSVLEPALP
                     RGSHVKDAWAYS"
     gene            complement(8242..8544)
                     /locus_tag="VF195_11250"
     CDS             complement(8242..8544)
                     /locus_tag="VF195_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581432.1"
                     /translation="MQERLRASARRDEMSVNQALDRLPSHPLTGDREVDREIASKEDP
                     SATSAPGDTAHRGRAVLAIAASAAAGILAGLASMKDPVRAARESDEEKSQSRLKGS"
     gene            complement(8592..9014)
                     /locus_tag="VF195_11255"
     CDS             complement(8592..9014)
                     /locus_tag="VF195_11255"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX6581433.1"
                     /translation="MPEQSDDLRDLLRQLSRDGRVLLQQEAELAKAELRDKSKTVVRS
                     AALFGGAALLGLIAAGALTASAVLAIALILPPWAAALIVATASALAAMLLALRGRQRL
                     QTAMPLLPERAIASVQEDVEWVKHQTRSTRTSRQYARG"
     gene            9264..9611
                     /locus_tag="VF195_11260"
     CDS             9264..9611
                     /locus_tag="VF195_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581434.1"
                     /translation="MAGTVVGDRVRSEIGARSSEIAIETKALAQAMREASRSLKEQGH
                     EAQASLAEDIAGRADRLAAYLRRSDATTLIADGKTWGREASEFAKREPLLVTAGAFTL
                     GLLATRWLGSDHS"
BASE COUNT         1798 a         3574 c         3774 g         1776 t
ORIGIN      
        1 agctcgaagt ggttcggggc ccagacctcc gagacgccga cgctccggcc ggcctccatc
       61 tcccgcatga tcagcttcgc gatctcgacg ggttgcacgc cgctgcggaa ggccttggag
      121 aacagacctt cgacgagtcc accgaggcgt cgttcgaagt cgcggaggat cggcacgatg
      181 atccggtggg cgcgtggagt ggaacgcgct ccgcgcctat cctaccgccg gggttccggt
      241 tccccttcgg cgcctcggtc ggcaccgttc cggctcgtct ccagctcggc cgagcgcggg
      301 tgctcccaga tcgggctcgc gaacccgtac cgctcgtccg gtgggagccg ccgccagcgc
      361 cattcctgga tctcccagta ggtccggcgc gcgaagacga gcacgaggac cgccaggagg
      421 aaccggaggg tcgcgctggg cgagatctgc aagacgccga gcgccatgcc tgtcgcggat
      481 gcggccaccc acgcggtcag ggccacgaag ccgaccacca tcaggtggcg catcaatccg
      541 tcgaagtcca cgttcgcctt cgtgctcatc ccggaagagg tatcgacgcg gaaccgtgga
      601 gaactggata ggtcggctgt cgcaagtcac gttggtccgg gtccgcacgg gccctcggcc
      661 gcccggccta cggccagccg cccgagggcc actgttacga tggccggacg ctcgggcgag
      721 tggcggaatt ggcagacgcg cacggttcag gtccgtgtcc ggtaacccgg gtgggggttc
      781 aactcccccc tcgcccacca gctccgacag agttccctct ggcggaacag agggacgggg
      841 atcatcggaa ctcggaaggt cgaggctcca ccgtggctcg ggatcgatac ggacgggttc
      901 ttcgggttcg gtcactgacc tctcgccggt cggcccgaac gagcggcgta tactcgggat
      961 tcccgcggat ccccacgtag atgacgctct ctcctttcat cgccgccctg gtgctctcgc
     1021 cctccaccct gacgctggtc gcgctcgtgg ccatcgggct cgccatcgtg gcggtgctgt
     1081 actcgatcgc gaacgcgggg aaagccgggg gttccgtcgg cggacgtgtc aaactcgatg
     1141 agacgctccg aggcatcctc caggggcaag gggagcagat gcagcggctc gaacgcgcga
     1201 tccgcgcgct ccatgccacc ggcaagaagc agcatgcgga gatcgagggg tccgtgcgga
     1261 acgtggccct gctccgctac gacgcgttcg aggacgtcgg cggccgcctc tcgttctcgt
     1321 gcgcgctgct cgatgaccac ggtgatgggg tcgtcctgac atcgatcaac ggccggcagg
     1381 agacgcgtgt ctacgcgaag caggtcaccc aggggacgag ctcgcacaat ctgtccctgg
     1441 aagaggaaga agcgatccgg cgtgcgatgg ggggacggca agcgaccgtc gagaccggat
     1501 gatccggcga cgggacgacg tggaaggtcc gggccccggc ggcggtccag gccccgatgg
     1561 aacggaggca ggcatggctg cagagaacgg agaggtcacc atcgtcggcg cgggggccag
     1621 gctggagggc aacgtcgtat cggccgggaa cctgaggatc gacgggcagg tgaaggggca
     1681 gatcaacgcg gagggcgacg tagcgctgtc gccgcagagt caggtcgagg cggacatccg
     1741 tgcccagaac gtgagcatcg ccgggcggtt caagggctcc atccaggtga acggtaaggc
     1801 acacctggcc cgcgggggcc gcatcgacgg caacatcacc tccaagacgc tcgtggtcga
     1861 ggagggcggc gtgttccacg ggcagagcat catggacggc tccgctccca ccgaggccgc
     1921 tcgttcgcag ggtgccacgc cggcgaaagg gccagagaac gagccagaga acgcgccaga
     1981 gaacgcacag gagaagcggg acgaaccgga agtgaaggtc ccgtagttgc tcgacatcag
     2041 acggatcagg gaagatcccg atccgttccg ggccgctctc gcgaggcgga acctcggtga
     2101 tgccgtggac cggctgctcg aggcggacga gcggcgccga tcgctcacgt cccgcgtcga
     2161 ggagctgcgc gcgacgcaga accgtgcgtc gaaagcgatc ggcgccgcca agggtgacga
     2221 gaagcagcag cttatcgacg aggtcgcgaa ggtttccgcc gagctcaagg atctggaccc
     2281 ggagctcgcg gcggccgagg agggcctccg ttccctcctg gcggagatac ccaaccttcc
     2341 gcacgagagc gcccccgatg ggttcacgga cgaggacgcg gtcgaggtca gacgcaacca
     2401 cgaggaaccg ccggtcctcg ggttcgagcc tcgcgatcac gcggagctcg gcgcgatgct
     2461 cggcgtgctc gacaccgagc gcggagcgcg gacatccggt tcccggttcg tctacctgat
     2521 gggtgatctg gtcttcgtgc agttcgcgtt gatgcgccac gcgatggatg tcgtcgtcga
     2581 caaggggttc tcgccgatga tcccgccggt cctcgtccgc gaggaggcga tgtacggcac
     2641 cggattcctg ccgacggacg ccgtgaacat ctacgtgacg cgcgaggacg agctctacct
     2701 cacggggacg tcggaggtgg ccctggcggc gttccacctc ggcgagatcc tcgacgaagc
     2761 cgacctcccg atccgctacg ccggctactc gagctgcttc cgacgcgagg cgggttcgta
     2821 cggcaaggac ctggggggca tgttccgcgt ccatcagttc gacaaggtcg agatgttcgc
     2881 gttctgcacg cccgagacgt cgtgggacga gcacgagcgc atcctcgcca tcgaggagga
     2941 gatcctcggg aacctggaga tcccctaccg ggtggtgaac gtcgcggcgg gcgatctcgg
     3001 tggctccgcg gccaagaagt acgacatcga ggggtggctg ccggcgcagc agcgctaccg
     3061 cgagctcacc tcgtgctcga acacgaccga ttaccaggcg cgccggatgc ggacgcggct
     3121 ccgccgggcg ggtggggacg tcgaggtcct ccacaccttg aacggcaccg ccacggcgat
     3181 cggccgaacg ctgatcgcga tcatggagaa ccaccagcgc cccgatggca gcgtggagat
     3241 ccccgcgaag ctgcatccgt acctgccgga gcgcgcgcgc gtgcttgccc ctcggtcctg
     3301 acccccaggt ccgcctgatg tggctctact gagctgggtc gaccacggcg tcgcccgccg
     3361 cgactccctc gtccgctccg gcctctgctc ggtacgcggt cacgtcgatc gcgacgccgt
     3421 gccaggtctc ggggatcacg ccggaagccg aggtgcgccg cccgaacgag tggagccatc
     3481 ggatctcgcc atcgcgccgc aggatccgat acgtgtcgtc ccaggcgccc gtctcatcgg
     3541 agcgctgaat cgactgatgc acccggggac gatcgtccgg atgcaccatc cgagggaagt
     3601 gcttcggctc gtccatgagc tcctcgggtg tgtatccgag gatgtcgaag gcctgagacc
     3661 cgatgtacgt gtaccgctcc acccccgtgt cggggtggat cgtctcggac caggggatcg
     3721 cgagcgcccc ctccagcgcc tcgcgctgag ctcccgcggt cgcctcgacc tgggccctcg
     3781 cttcttcatc cgtggtcacg tcgaggagga tgccctggaa ggtccacggt ctcccccgcg
     3841 tgtcccgatc gaggaggcgg ccggcgtcga ggagccagat gagcgttccg tcggagcgga
     3901 tcatccggta gcggagcatc cacggctcgc cggtccgcca gttgtgcgcc gtcatctggg
     3961 cgacgcgttc gcggtcgtcc ggatggatca tcccgaacca gacctcgggc tcatggagcc
     4021 agtgcccgat cggatactgg acgaggtccg cggcctgcgg gctcacgtgc gagacgtgga
     4081 gggaggggtc ggccccgtcg acgtaggaca gctcgtagga gtaggcgacg accgggccgc
     4141 gctccgtgag cgtccggtag cggtcctccg cggccgcagc cttggattcg gcctccatgc
     4201 gactcgtgac atccagcatc atgccctgga aggaagccgg gtcaccggtg tcggatcgcg
     4261 agatcagcga ggcgtggtcc aggacccaga ccacgctccc gtccttcgcg aggtaccggt
     4321 actccatgac gaaggcctcg ccggtccgca cgctccggtc gacggcctcg gagacgcgcg
     4381 cttgatcgtg cgggtggacg cgagccgacg cgtgccactc ctcgggtgag aatccgagga
     4441 tgtcttcgat ccgggggctc acgtacccga aggttcgggc ctcggccccc gggcggactt
     4501 cccagacgta cgcgatgccg ggcatgttct cgaccagccg gcggtatcgt tcctcgacgt
     4561 cacggagctc gagctccatc cgccggcgct ctccgatctc gagcgacatc agctcgttgg
     4621 cttcccgcag ctcactggtt cgctctgcga cgcgccgctc caggtcctcc ttgaggagga
     4681 gcagcatctc cgtcgcctcg tgctccgcgg tgatgtcgat catgacgccg tgccacgcgg
     4741 ccgcgccgcg ctcgagaccg cggatcaagc tcgcgcggtc gtggacccag acgaccctcc
     4801 cgtcgctcgc gatcaaccgg tactccaggt cccacggctc ccccgtctcg ttgtggcggt
     4861 cgtgcttcgc gaggacgacc tcacggtcgt ccgcgtggag gagctcgatc cagatgtcgg
     4921 gctggtcgag ccattcctcg cgcgaatacc cgagaacctg ttcgacgtgc gggctcacga
     4981 acagcgtctg gcgttcgtcg tcggggccca tctcgtacac gaccgccggc acgcgctcga
     5041 cgagagctcg atagcgagcc tccgatgcac ggatctcctg ctcggctcgc cgctcggtcg
     5101 tgatgtcctc gatcacgccc tgccacgcga ggcgctggcc ctcatcgctg aggacgagca
     5161 cgctcgagtc ccggagccag acctcctctc cgtcgcgccg gaacatccgg tactccgacc
     5221 ggtagcgcgc cccgctcgac cagacctcgt cgcggatcgc ctggatccgc tcccggtcgt
     5281 ccgggtgcat acagcggatc cagagacccg gatcctccag gaaccgctgc ggcgggaacc
     5341 ccaggagttg ctggacgttg ggactgatgt aggtcgtcga cggacgctgg tcgtcggtgt
     5401 ccacgtagac gacgacgggg agctgctcga cgagctcgcg gacccgcgat ccagacgcga
     5461 acggatcgag gggctcgggg gcgcgggaat gcgggtgacc cggcatcgaa ccagggtacc
     5521 ccgaccgcca tctcccactc cagggctgac gtaggctcct cgcgggaggg gaaacgtggg
     5581 cactgcctgc tcgcccggtg cgtacatcgg ggctgcggga ctcgcactcg ccggtatcgc
     5641 cctgatcgcc tacttcgtgg gcggccaggg gtggcgaacc cgaaccaaag tcatcctctt
     5701 gggtggcgcg ggtctcctca gcgcggttct catcgtgaca ggggtcgagc tgaagctccg
     5761 agaggaacat gacgtcgtga agagcgctac gggtacggaa gtcgtatgcc atcacgtgta
     5821 gggcgcgcgg ttctgcagcc gcatggcacc gacggagtcc attgagccgc aggtagcctg
     5881 tgcgccggga ggatggccga gcggacgaag gcgacggtct tgaaaaccgt tagcggggca
     5941 actcgctcgt gggttcgaat cccactcctc ccgccgagct ccgagccgtg accgcatgtt
     6001 cgtgcgtcac gaccacgcga ggctcgtgtt cgggatccgc tctctccctc gctcggttcg
     6061 accagctacg agtacgccca ggcgtccttc acgtggctcc cacgcgggag ggctggctcg
     6121 aggaccgagc tggttgcgcg tcgccgtctc gacgctgtga gcgcgagtcc gcccaggaag
     6181 gccagcaccc cagcggctgc gacccacggc aggagcgtga gaccggtgag cgccaggatc
     6241 ctgatgatcg aggcgaccga ctcgacgagg gaatcgatcc cgtccgcagc ccccgtcacc
     6301 gcgcagctca gactcgaagc accgtcacag gtggccaccg gctcgttccc ctggagccgc
     6361 tcgccctcga tctggcctcg gttcacgctg aggagccgct cggtcccgat cggcgagctc
     6421 cctccctgga tcccccacac ccggccgaac gtcatcggtc ccgagatcgc gccctcggaa
     6481 gcctggtcgg tgctccctag gctcgccggg tagagatcgg acatgacggt ctccgtggcc
     6541 tcgttcagga ccgtcatcgg atcggccagt ccgtcatcga tcccgtccgc cacgaccccc
     6601 gcggatgtcg cctccgaaag ggtcgctacg ggattggagg ttccgtcggg tccagctccc
     6661 accgtctcgg acgtgatctc cgatgccatc ccgacgaccg ggtcagccgt gccggagccc
     6721 tcggtcatgg cgtcgacgac cgtaaccgcg gtctcggggt tcgtgtcagc gggggtttcg
     6781 gcgagggtca cctgcgcctc ggttgctgtc tgaccgagga gtgcggtgac gtcggtcgtc
     6841 gcctcggagg tcgtcgtggt ggagtcgacg atgatctgag tggccgggtc ggagaacgcc
     6901 aacacctctt cgacgcccgt gatgatctcc ggcgatgtgt cggacaccgc tgtggccgcg
     6961 tcggcgatgg ttgacgttgt gcccgtcacc gcttccaggg atgccacgtt ggatacggtg
     7021 gccgacgcct ccgttgccac ggtctcgaac gtcgcccatg tcgcgtccgt cgccccgccg
     7081 atgaccgcgg acgactcgac ggtgatcgcg gttgcttccc taaccgcggt ctgggtgatc
     7141 gcctccgcgc tcatcgtcgc ccccgccgtt acatcggtcg tcatccctgc cgtatcagag
     7201 gtcgtctcga ccatcgacgc cgcctccacg gtggcgtcgc cgaccgctgt cgcgacctcg
     7261 gacgtcgcat cggggatcgc tccggtgcct tccgtcgcct ctgggacaac aaccgcatcg
     7321 gtcgcttcgg tggacgtgtg cgcgatcatc gccgccacat cggtggcggt ctcggatgcc
     7381 gcggccgtcg cgtcaaggga cggctgctcg tccgatccgg cctcagctga gatcgtgccg
     7441 gttgtcgcat cggccgtagc atccgcgctc acggcgaccg cgtcggatgt cgtttcggtc
     7501 acgatgtcgg gggtggccga gatcgcgttg gttgcggcat cctcgtcggt tgtcgcggag
     7561 gtcacgtcag tcaccgttac atcgaccgct tggggcgctt cttggggtgc ttcgagcgtc
     7621 tcggaggcgg catctgtccc accgaccgga tcttgggatg tcccgtcgga aaccgcgtcg
     7681 gtcacgatgc cggagagggc gtcgggagcc gcgaccgtcg ttcccgtggc gtccgtgtct
     7741 tcgatcgccg tgtcggcggt tgcagcggtg ggatccgtgg tggcttcgat ggatgcgtcc
     7801 accgaggccg cttggaccgg atcggtgatg tcggaggtcc ccactccgtc tggggcagcg
     7861 ctcgcatcgt cggtcgtggc gtcgacggtg gcggtaaggg cgttggtcgg aggtgtatcc
     7921 gcgaaggctc ttggggccga agctccgagc aacgcgatcg cggaaacgag acacccgatg
     7981 gcgaggcccc tgcctcgtcc agtccaccgg tcgagacata tagatccgtc ctcagccgac
     8041 atctgccgcc ttcgttcgca taccctgagg gtggaggtct cattcagaag cggtaggtga
     8101 gattccctac gcacggttga tgtagtggga gtacggtccg cagtgagaac cgccatcctg
     8161 gctgaacgtc cgcagcaggg ttcgtccgcg cggggggaca agagtgcgga tcggttcaag
     8221 gccccacacg acgtggcgag gtcacgatcc cttgagtctt gactgactct tctcctcatc
     8281 cgactctctt gccgctcgga cgggatcctt catcgaggcg agccccgcca ggatgcccgc
     8341 ggctgcggac gcggcgatcg cgagcactgc ccggccgcgg tgcgctgtat cgccaggcgc
     8401 cgacgtcgcg gacggatcct ccttggaggc gatctcccga tccacctcgc ggtcgccggt
     8461 gagggggtgg ctggggagtc gatccaaggc ctgattcacc gacatctcgt cccgcctggc
     8521 actggcgcgg agacgctctt gcacccggct cctgatatct gccgtaaggg agatagcgcg
     8581 gatcgtctcc gtcatccgcg cgcgtactgt cgcgatgttc tcgtggatct cgtctggtgt
     8641 ttgacccatt cgacgtcctc ctgaacggaa gcgatcgcgc gctcgggcaa aaggggcatc
     8701 gctgtctgca gccgctggcg accgcgaagc gcgaggagca tggcagcgag cgccgaggcg
     8761 gtagccacga tgagggccgc ggcccagggc gggaggatca gcgcgatcgc gaggactgcg
     8821 gatgcggtga gggcacccgc tgcgatcaac cccaacagcg ctgcccctcc gaagagagcc
     8881 gccgaccgta cgaccgtctt cgacttgtcc cgcagctccg ccttcgcgag ctctgcctcc
     8941 tgctggagga ggacgcgccc gtcccgactc aactgtcgga gcagatcacg aaggtcgtcg
     9001 gactgctcag gcatggcata ccctttcgcc tctcggcact ccatcagctc accgcggcat
     9061 tacccaacga cgtacgtcgc tactccgcgt cggcaaggtt tccccacgtc ctcccgaggg
     9121 aaaggcggat gcaccggcgg atccgacatg acctagaggg acctagaggg cggggtgttg
     9181 acatggccca gaagagcagc gatcagggag cgggggccca gatcaaaggg cagctacaag
     9241 aggccactcg gcttgtttcc gacgtggcag gaaccgttgt cggtgatcgc gttcggagcg
     9301 agatcggcgc tcgatcatcg gagatcgcca tcgagacgaa ggcgctcgcg caagcgatgc
     9361 gtgaggcgag tcgcagcttg aaggagcaag ggcatgaggc gcaagcgagc ctcgcggagg
     9421 atatcgccgg tcgtgctgat cggttggcag cgtacctccg taggtcggat gcgacgacgt
     9481 tgatcgcgga cggcaagacg tggggacgag aggcgagcga gttcgccaag cgggagcctc
     9541 tgctggtcac ggccggggcg ttcacgcttg gtctgcttgc taccagatgg ctcggcagcg
     9601 accattcata gacgggacgg ggaggcggta gcgatgccag gggtaacgga cgtcaaggca
     9661 cgagttgcga aggcgggaga ggccgtggag gcggtgggaa gcggcgccaa gcgagtcgcc
     9721 acaggtggag gcaaggtcat gaagcgaggg ggccacgcgg ccagcgatgc cgccgtgtca
     9781 gccggtaagg gggtgaagaa agcggccgtt gcgatcaagc agaacccgtt ggtagcggtc
     9841 gccgcggccg gcgccgtagg cgcagcgatc ggagaagcga gcaagcgatc caggaggact
     9901 cctcgcgcgg gctcgtctgg aaggaagccc gcgaggaagt catcggcgaa gcctgtttcc
     9961 aggtcgaaga caggatcgag aacccgtccc actcgcacgc ggacagccaa gacgacctct
    10021 ggtcgcaaga cgtcggcggc gaggactccg gcacggaagc gcacggctgg gaagaccagt
    10081 acgaggaaga gcgcaacgaa gaagagcaca gcgaagaaga gcgcacctcg cgcggtcgcg
    10141 aagacatcga gggcggccaa accgacctct ggcacgagga agcgaacgtc agcctcgcga
    10201 acccagcgtt aacacggggc ttcgtcggtc cgccccgccc ttagtagggc ggacccgtac
    10261 ggactgcctg agctcgactc gggtacgccc atgtacctgc tcggtccgcc cgcccccggt
    10321 ctgggacatc tgtacgggag ctggtggctg gtcgcctacg cttcccaggt atgacgaaca
    10381 gagcgaaggt gacgtctact cggggtgcac gtcgtctctc ggacggacga ggcatcgtga
    10441 aggtgtcgga gacgacgcag cggatcctga acggcaccga agacctctcc atctggtcgg
    10501 atgaagagtt tgatgcgcgg tgtccggcga gggaaggacg gcaagttccg caacaagcct
    10561 aaggtcatcc gcacgccgtt caccaagaag ctcgtgaagc gccgtctgag tcgcgcctac
    10621 gacttgctcc gtgagagcgt cgtgcgggga gcgtcgtttt acgttgcgac gcaagcgtgt
    10681 cgcaggtagg ggtggacgaa ggggtcttct ggaaaaccgt tagcggggca actcgctcgt
    10741 gggttcgaat cccactcctc ccgccaaggc tcatgtgtct gcccgggtga accgcaagat
    10801 cgtgacgaac gtccgcagca cgactcggca ccagccgcgg ctgcgatcgg ccagggcgac
    10861 tattccaaag cacgtgcgtg gtcgcccgtg tatgcatggc aagagggagg ccgcggacct
    10921 cc
//
LOCUS       DASUYD010000208         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1906947, whole genome shotgun sequence.
ACCESSION   DASUYD010000208 DASUYD010000000
VERSION     DASUYD010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1906947"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF195_11265"
     CDS             <1..199
                     /locus_tag="VF195_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018635347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581435.1"
                     /translation="KLTDREMEVLRLVAQGLNNRDIAKQLFISENTVKNHIRNILEKL
                     HLHSRMEAVVYAVREKMIEIT"
     gene            219..695
                     /locus_tag="VF195_11270"
     CDS             219..695
                     /locus_tag="VF195_11270"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6581436.1"
                     /translation="MGDPTIEAARELFDEAFGQMRDAIDGLSAEALAWRPPAEDTNPI
                     TVLAVHSMHSTRWWLTIARGLPLPDRDRPSEFVAETGGDAELLGFLDRMVSECRERLD
                     PAEAFDAGAERSVLDDEPVTAGWALLHALEHLREHVAQAQLTRQLWDMHEDLKGSG"
     gene            816..1205
                     /locus_tag="VF195_11275"
     CDS             816..1205
                     /locus_tag="VF195_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581437.1"
                     /translation="MGFGLVLVAAILAPSLAADAESVPSKDPGEEFAKSAGCDRDRAD
                     VPMNVSFFSQHASPGEAAVGAPTVAEAALDVASDTYSPATVVEAAQEAVAGSGSDSIV
                     VRLDGASIEIARLPDGMFVAVGLIECV"
     gene            complement(1390..1989)
                     /locus_tag="VF195_11280"
     CDS             complement(1390..1989)
                     /locus_tag="VF195_11280"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEX6581438.1"
                     /translation="MEFRDVVRHRRMVRRFDRRPVPHDVIDRVIDVGRRAPSAGFSQG
                     LELLVLDTPETIATFWETTRDPEFGWEDETVAHGPTVLILPLPDPRRYLDRYAQRDKI
                     DFGMDEEANWPVRFWEIDAAMASMLILLAAVDEGLGGWFFGVTHGERELLDRFGVSGD
                     LRPIGIIGLGYRAADEEPTGSWMKRRRRPLPEQIHRNGW"
     gene            2033..>2148
                     /locus_tag="VF195_11285"
     CDS             2033..>2148
                     /locus_tag="VF195_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015171322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX6581439.1"
                     /translation="MDDAQGKRTVARLLVECLENEGVGYVFGLPGEENLHVI"
BASE COUNT          355 a          713 c          745 g          335 t
ORIGIN      
        1 caagctcacc gatcgcgaga tggaggtgct ccgcctcgtc gcgcaagggc tgaacaaccg
       61 ggacatcgcc aagcagctgt tcatctccga gaacacggtc aagaaccaca tccgcaacat
      121 cctcgagaag ctccacctgc actcgcgcat ggaagcggtc gtgtacgccg tgcgggagaa
      181 gatgatcgag atcacgtgag acgccggtaa cccccgcgat gggcgatccc acgatcgagg
      241 cggcgcgcga gctcttcgac gaggctttcg ggcagatgcg cgatgcgatc gacggcctct
      301 ccgcggaggc cctcgcctgg cggccccctg cggaggacac gaacccgatc accgtccttg
      361 ccgtccattc gatgcacagc acccggtggt ggctcacgat cgcgcgcggc ctgccgctcc
      421 cggaccggga ccggccgtcg gagttcgtcg ccgagacggg cggcgacgcc gagctcttgg
      481 ggttcctcga ccggatggtg agcgagtgcc gggagcggct ggatccggcg gaggcgttcg
      541 atgccggcgc cgagcgatcc gtgctcgacg acgagccggt gaccgcgggg tgggccctcc
      601 tccacgcgct ggagcatctc cgggagcacg tcgcgcaggc gcagctcacg cgccagctct
      661 gggacatgca cgaggacctc aaagggtccg gctgatgtgt gcacgtttcc gtcgtctggt
      721 cccttgacgg aacccgccac gagcggtgca atccattgca catgggggaa acgcgagaag
      781 gggcccgagc aacgatgggc agttggggcg tcagcgtggg cttcggcctc gttctggtgg
      841 cggcgatcct cgcgccgagc ctcgcggcgg atgcggagtc ggttccttcg aaggatcccg
      901 gcgaagagtt cgcgaagagc gcgggttgcg atcgcgatcg cgccgacgtt ccgatgaacg
      961 tctccttctt cagccaacac gcatcaccgg gtgaagcggc ggttggtgct ccgaccgtcg
     1021 cggaagctgc gcttgatgtg gcatccgata cgtactctcc cgcgaccgtg gtcgaggccg
     1081 cgcaggaggc cgttgctggc tcgggcagcg attccatcgt tgtacgtctc gacggggcgt
     1141 ccatcgagat cgcccgtttg ccggacggga tgttcgtcgc ggtagggctt attgagtgcg
     1201 tctgacggcc aaccatgtag ccacaggtga gggccggcat tccgggacgc agcggatgat
     1261 cgcctcgcga gcgtgccaca gctcggggga gctcttgttg tcatcggtcc tccctcgagc
     1321 cgcgagccga tgtgaacacg atgttggtca gtgccgaaaa gggagcgagg aggtggcacc
     1381 gtcgcccgct caccagccgt tgcggtggat ctgctccgga agcgggcgac ggcgccgctt
     1441 catccacgat cccgtaggct cctcgtccgc tgcgcgatac ccgagtccga tgatcccgat
     1501 cggtcggaga tcgccggaga ccccgaaccg atcgaggagc tcgcgctcgc cgtgcgtgac
     1561 gccgaagaac cagccgccga gcccctcgtc caccgccgcc agcaggatga gcatcgaggc
     1621 catcgccgca tcgatctccc agaaccgcac cggccagttc gcttcctcgt ccatcccgaa
     1681 gtcgatcttg tcgcgctgcg cgtagcggtc gaggtaccgg cgcggatcgg ggagcgggag
     1741 gatcaggacg gtcggcccgt gcgcgacggt ctcgtcttcc cagccgaact cggggtcgcg
     1801 ggtcgtctcc cagaacgtcg cgatcgtctc cggggtgtcg agcacgagga gctcgagccc
     1861 ctgcgagaac cccgcggagg gggcgcgacg ccccacgtcg atcacgcgat cgatgacgtc
     1921 gtgcgggacg ggccgccggt cgaagcgccg caccatccgc cgatgccgca ccacgtcacg
     1981 gaattccacc ggccgagcgt accgacccgg accgccgggt agcctgacgc cgatggacga
     2041 cgcgcagggg aagagaaccg tggcacgcct cctcgtcgag tgcctcgaga acgaaggggt
     2101 cggctacgtc ttcggcctgc cgggggagga gaacctccac gtgatcga
//
LOCUS       DASUYD010000209         3909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_583618, whole genome shotgun sequence.
ACCESSION   DASUYD010000209 DASUYD010000000
VERSION     DASUYD010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583618"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..448)
                     /locus_tag="VF195_11290"
     CDS             complement(194..448)
                     /locus_tag="VF195_11290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581440.1"
                     /translation="MGSVIHLAEWRQRREHDPMSRLDRAIARLDGLLRRSGSGRLAGR
                     VESELLAITGAVSAGLEDEAAERAERLAERLEHPSARRSG"
     gene            543..1376
                     /locus_tag="VF195_11295"
     CDS             543..1376
                     /locus_tag="VF195_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581441.1"
                     /translation="MGRTLSLVPLVILIALVPASSANAGGWDSLVFPDDHYLVGEVAT
                     TTQWFFAGDLRGAGPLDGRLYHAYLLPRGATERGLGMITPPTIPAGAIELSTLQTTGP
                     FERPHYKGPYGRATLTFTVPEVPTGRYSIGFCDDPCEHGSVGWLAWATITIVHTEEEG
                     RLLASLGRMDRQAWKLRSDLRRAERASQDLQDEAAALGEDVRERTLSARAAGTRIAAL
                     EAVGTPAPESRPLIPGWAAGMLAAAIIAAAVIARRRRSDVFVVPNTVPDDLVERDRAG
                     V"
     gene            1451..1984
                     /locus_tag="VF195_11300"
     CDS             1451..1984
                     /locus_tag="VF195_11300"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX6581442.1"
                     /translation="MLTGDRVVLRPIQRADLPRLWELIGDFEVAVLGTSGPVLPQSLA
                     EFEATFDHDPAQPGKDRAYFAIELDGELIGEAGLHRIDHFSRTCELGIGIGRDYWGKG
                     FGQDAVRTLIDYAFEHLNMNRVGLYVLAEDPRAVGAYRKAGFVEEGRIRQHAWVRGRY
                     EDELVMSILREEWSPST"
     gene            complement(1981..2337)
                     /locus_tag="VF195_11305"
     CDS             complement(1981..2337)
                     /locus_tag="VF195_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006350594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6581443.1"
                     /translation="MTGIEIRRFEQADETRKFEHGSFEVVTIGDLTIGRASYEPGWIW
                     SEHVGKPAGEELCQVEHVGLVVSGRTGVRMADGTEAELTPGDLFAIGPGHDSWVVGDE
                     PYVSLHLLGASEYAKH"
     gene            2524..3600
                     /locus_tag="VF195_11310"
     CDS             2524..3600
                     /locus_tag="VF195_11310"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6581444.1"
                     /translation="MEEVARIVLALESPDVAEEVMHFLDRTGRARVVGTAVDASQLAE
                     AIRQLEPDALVAAPALVPSRGTLNGSALLVVDTSQSVGALRRAIRAGASGFYLWPAER
                     EELAIAAARVGPTREESVGEPAPVLAVYAPRGGSGGTFVATHLAAAFAKRQRRCVLVD
                     LDVQFGDASAAIGVPTDEAVRTIADLAPLREELGPHHVEEVLWRHPKGFAALLAPGDG
                     HLSQRLGAETYRAAISAVRRSCDVVVLHVPRALDEVTRSGLELADRVLVVLGLDVLSF
                     RDAKRAIEVAGLDGKCSFVVNRAGRSEIAPDDVERVFGQRPLVVIPSDRAVGVAQDHG
                     RLIPMRGRVGRALDRLAQQTQEPA"
     gene            3597..>3909
                     /locus_tag="VF195_11315"
     CDS             3597..>3909
                     /locus_tag="VF195_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581445.1"
                     /translation="MNRTDALVDVRRSLHERLIGRVDARQLDAMPRAERRLRVREEAL
                     SLLREQGHMLPQSTLADVVNAVSDAVVGMGPIEFLLKDPDVTEVMVNGPDDVYVERKG
                     RI"
BASE COUNT          560 a         1310 c         1399 g          640 t
ORIGIN      
        1 gttcgcctgt cctggccggc cacccagcgt agccccgcac cggcacgcag gcagcgttgg
       61 gcgcgagcgt gcgccggggc gcctcctccc ggaagcttcc ggtccgctct tgatgaccga
      121 gccgagcccg agcgggttcc aagcgtgctc gacctcgtgg tggccgagcc cctcgtgggg
      181 ggtcgatccg tgcttagccg gagcgtcggg cggagggatg ctcgagccgt tcggccagcc
      241 gttcggcgcg ttctgcggcc tcgtcctcga gccccgcgct cacggcgccg gtgatggcga
      301 ggagctcgga ttcgaccctg cccgccaggc gcccggaacc gctccggcgg aggaggccgt
      361 ccaggcgcgc gatggcgcga tccaggcgcg acatcgggtc gtgctcgcga cgctggcgcc
      421 actcggcgag gtggatgacc gaacccatga ggcgatcgta gacccggggt ctgacacttt
      481 cccggaaccg ggggcggttc gggccggtcg ggtcggttgg aagccgagct tcggaggtgc
      541 ccatgggccg cacgctctcg ctggtcccgc tcgtgatcct gatcgccctc gtgcctgcgt
      601 cctccgcgaa cgccggaggg tgggacagcc tcgtgttccc cgacgaccac tacctcgtgg
      661 gcgaagttgc aacgaccacg cagtggttct tcgccggcga tctacgaggg gcggggccgc
      721 tcgatgggcg cctctaccac gcgtacctcc tgcctcgagg tgccacggaa aggggcctcg
      781 gcatgatcac cccaccgacg atcccggccg gtgccatcga gctgagcacc ctccagacca
      841 cagggccgtt cgagcgccct cactacaagg gtccgtatgg gcgggcgacc ttgaccttca
      901 cggttccaga ggtcccaacg ggtcggtact cgatcggttt ctgcgacgat ccgtgcgagc
      961 acggatccgt tggctggctg gcatgggcca cgatcaccat cgtccacacc gaggaagagg
     1021 gtcgtctcct cgcgtcactc ggtcggatgg atcgacaggc gtggaagctc cgcagcgatc
     1081 tccgccgtgc ggaacgggcg tcccaggatc tgcaagacga ggcggcggcc ctcggtgaag
     1141 acgtgcgtga gcgcacgctc tcggcgcggg cggccggaac caggatcgcc gcgctggaag
     1201 cggtcggcac gcccgcgcca gagagtcgac cgttgatccc cggctgggcg gctggcatgc
     1261 tcgcggccgc gatcatcgcg gccgcggtca tcgcccgacg tcgccgttcg gacgtgttcg
     1321 tcgtgccgaa caccgtcccg gacgacctcg tcgaacgcga ccgcgccggc gtgtagtggt
     1381 tcgctcatac gcgagggcag accgcgtagg ctgctgcgca catgctgtat gtcggtgtgc
     1441 gtgtgccacg atgctgaccg gtgaccgcgt ggtgctccgt ccgatccagc gggcggatct
     1501 gccgcgactg tgggagctga tcggggactt cgaggtcgcc gtcctgggga cctcgggccc
     1561 ggtcctcccc cagtcgctcg cggagttcga ggcgacgttc gatcacgatc cggcgcaacc
     1621 gggaaaggat cgcgcgtact tcgcgatcga gttggacggc gagctgatcg gagaggcggg
     1681 cctccaccgg atcgaccact tcagtcgcac gtgcgagctg gggatcggca tcggtcgcga
     1741 ctactggggg aagggcttcg ggcaggatgc ggtccggacg ctgatcgact acgcgttcga
     1801 gcacctgaac atgaaccggg tcggcctcta tgtattggcc gaagatcctc gagcggtagg
     1861 ggcgtaccgg aaggccgggt tcgtcgaaga aggccggatc cggcagcacg cttgggtccg
     1921 aggcaggtac gaggacgagc tcgtgatgtc catcctgcgc gaggagtggt cgcccagtac
     1981 ctagtgcttc gcgtactcgc tcgcgccgag caggtggagc gagacgtacg gctcgtcgcc
     2041 cacgacccac gagtcgtgac cgggtccgat cgcgaacaga tcgcccggcg tgagctcggc
     2101 ctcggtcccg tccgccatcc tgacgccggt gcgtcccgag acgacgagac cgacgtgctc
     2161 cacctggcag agctcctcgc cggccggctt gccgacgtgc tccgaccaga tccacccggg
     2221 ttcgtagctc gcccgcccga tcgtgaggtc gccgatcgtc acgacctcga acgaaccgtg
     2281 ttcgaacttc cgggtctcgt cggcctgctc gaacctgcgg atctcgatcc cggtcatcgg
     2341 tcctcctcgc tctgtgacgc atcgcgtgga catcccccga caagaacgac gaatcggtcg
     2401 acctggtgat tgtctcgaac tcgcgtcacc gcgtactgtt cgcgcacgcg gttcgccgcg
     2461 actccccgaa gaagatcctc cctcaccggt tggtcggcgc ttccccttgg acgtggagcg
     2521 aggatggagg aggtcgcgcg gatcgtgctc gcgctcgagt caccggatgt cgccgaggag
     2581 gtcatgcact tcctcgatcg cacgggccgt gcgcgcgttg tgggaacggc cgtagacgcg
     2641 tcccagctcg ccgaggccat tcggcagctg gagccggatg cgctggtcgc cgcccccgcc
     2701 ctcgtcccgt cgcgtgggac gttgaacggc agcgcactcc tcgtcgtgga cacgtcgcaa
     2761 tccgttggtg cattgcgtcg agccatccga gcgggtgcga gcggcttcta cctgtggccc
     2821 gccgagcgag aggaattggc gatcgcggcc gcgcgggtgg ggccgacgcg agaggagagc
     2881 gtcggggagc cggcgcccgt gctcgcggtc tacgcgccgc gcggcggcag cggaggaacg
     2941 ttcgtggcga cccacctcgc ggccgcattc gccaaacgcc aacgccggtg cgtgctggtg
     3001 gacctcgacg tgcagttcgg ggacgcctcc gccgcgatcg gcgttcccac ggacgaggcg
     3061 gtgcgaacga tcgccgatct cgctcccctc cgggaggagc tgggtccaca tcacgtcgag
     3121 gaggtcctct ggcgtcaccc caagggcttc gcggcgctgc tggctccggg cgacggacat
     3181 ctgtctcaga ggctgggcgc ggagacgtac cgcgcagcga tctcggccgt gcggcgctcg
     3241 tgcgacgtcg tcgtcctgca cgttccccga gcgctggacg aggtcacacg gtccgggctc
     3301 gagctcgccg accgcgttct cgtcgtgctc ggtctcgacg tgctctcgtt ccgggatgcg
     3361 aagcgcgcga tcgaggtcgc ggggctggat ggcaagtgct cgttcgtggt gaatcgggcg
     3421 ggcaggtccg agatcgcccc cgacgacgtc gagcgggtct tcgggcagcg gccgctcgtc
     3481 gtgatccctt cggatcgcgc agtcggcgtc gcccaggatc acggccgtct gatcccgatg
     3541 cggggacgtg ttggtcgggc gctcgaccgg ctcgcgcagc agacgcagga gccggcgtga
     3601 atcgtacgga cgcgctcgtg gacgttcggc ggagcctgca cgagcggctg atcggccgcg
     3661 tggacgcacg gcagctcgac gcgatgccgc gcgccgaacg ccgacttcgc gtacgcgagg
     3721 aggccctttc gctgctcagg gaacaaggcc acatgctgcc gcagagcacg ctggccgatg
     3781 tcgtgaacgc ggtttcggat gcggtcgtcg ggatgggtcc gatcgagttc ttgctcaagg
     3841 accccgacgt caccgaggtg atggtgaacg gacccgacga cgtgtacgtg gagcggaagg
     3901 ggcggatcg
//
LOCUS       DASUYD010000210         3196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1908199, whole genome shotgun sequence.
ACCESSION   DASUYD010000210 DASUYD010000000
VERSION     DASUYD010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1908199"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..376)
                     /locus_tag="VF195_11320"
     CDS             complement(<1..376)
                     /locus_tag="VF195_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009481384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6581446.1"
                     /translation="MGDVKTFKMYIGGEWTDATDGNTRQIVSPANGDVIAEVPEGTAA
                     DVDRAVGAAKKAYDETWSDATPGERMRALLKMADLVEEHGDELGRLESENVGKVLALT
                     MSEEIPVIADQFRFFASGARVLE"
     gene            780..977
                     /locus_tag="VF195_11325"
     CDS             780..977
                     /locus_tag="VF195_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581447.1"
                     /translation="MLAIEDAIRDHGPFDEIVISTFPKGLSKWLKVDLPHRAEAAFGI
                     RVTHVVGEPESAVSGRHGRRR"
     gene            1054..2385
                     /gene="gabT"
                     /locus_tag="VF195_11330"
     CDS             1054..2385
                     /gene="gabT"
                     /locus_tag="VF195_11330"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017581868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6581448.1"
                     /translation="MDIPQERKVVTEIPGPKSREWFERRAAAVPQGVFNTHPIVTARA
                     SGAIIEDVDGNRLIDFATGIAVLNVGHTSPEVVAAAQRQLELDTHTCFHVTANEPYIE
                     LAERLNALAPGDAPKKTMFANSGAEAVENAVKIARKATGRQAVVTFDHAFHGRTLLAM
                     SLTAKVMPYKQGMGPFAPEIYRLPFAYPYRWPSDPERCAEEALAYARDEIHKHIGEEN
                     VAALILEPIQGEGGFIVPAPGFVKGLADLCSEHGIVFIADEIQSGMGRAGRWFAIEDE
                     GVVPDLITSAKSLGGGLPISAVTGRADLMDAVHVGGLGGTYGGNPVAAAAALAVLDKI
                     EREGLLERSRTLGERVLGRLREMQDRHDVIGDVRGRGMMTAIELVSDRKTKEPLGGPE
                     GNEIVKRCLEEGVVVLKAGTYDNVIRLLPPITIDEGLLDEGLETLNAALGS"
     gene            complement(2501..>3196)
                     /locus_tag="VF195_11335"
     CDS             complement(2501..>3196)
                     /locus_tag="VF195_11335"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX6581449.1"
                     /translation="GSRWAAAISEQQGSDCENVFVGRGLVQISGTQVRSDPARYWDYI
                     DAPVRAHYLKRVCIVGAESTGSTTLARSLAAAYATIWVPEYGREYTVEKFTIGTGDTW
                     LPSDFLRIALEQNALEDRMAREAPVGIMFCDTDSLATALWEEVYLGSTSEAVWRAADA
                     HGAAFALYLLTDHRDVPWEDDGLRLGDETRAAMTKRFEEVLSARSLPWVKVTGSRGTR
                     LAQAVEAITPFFE"
BASE COUNT          545 a         1088 c         1059 g          504 t
ORIGIN      
        1 cctcgaggac ccgtgctccg gaggcgaaga agcggaactg gtccgcgatg accgggatct
       61 cctccgacat cgtgagcgcg agcaccttcc cgacgttctc ggactcgagg cgaccgagct
      121 cgtcgccgtg ctcctcgacg aggtcggcca tcttcagcag cgctcgcatg cgctcgcccg
      181 gtgtggcgtc ggaccaggtc tcgtcgtacg ccttcttggc ggctcccacc gcgcggtcga
      241 cgtcggccgc ggtgccctcg gggacctcgg cgatcacatc gccgttagcg gggctgacga
      301 tctgccgggt gttgccgtcg gtggcgtcag tccactcgcc gccgatgtac atcttgaagg
      361 tcttgacgtc ccccatcgat gcctcccctt cgaacggttc gaacgccgga gcccgcgccc
      421 gcgggctctg tgagtccaag tatgccgaaa atcggcagat ctcggaccgc cggcgtgggg
      481 gtttcgcaat cgcgcagccc gtcataccgt tgacggcagg gaggtggtgc tcgcgcagcg
      541 ctaccttgtg gtggccaacc agaccctcgg ggccgaaccc ctcgccgtga ggctcagcga
      601 gttctcccgg cgcggtcggg tctccttctt cctcgtggtc cctgccacgc cgccacgcga
      661 tcacgtctgg accgaaggag aggctcgggc gacggccagg gccagcctgg acgcggcgat
      721 ggctcggttc gtcgaactgg acgccgattt tcgagggcga ggtcggcgac ggcaacccga
      781 tgctcgcgat cgaagacgcg atccgagacc acgggccctt cgacgagatc gtcatctcga
      841 cgttccccaa gggcctctcg aagtggctga aggtggacct gccgcaccgg gcggaggccg
      901 cgttcgggat ccgcgtcacg cacgtcgttg gggagcccga gtcggcggtt tccgggcgac
      961 acggtcgcag acgctgaacc ctatcggaca tcgttctcgg cccacgccgt ggcgtgacag
     1021 actgagcctc gaccaacacg aggaggcgcg agcgtggaca tcccgcagga acggaaggtc
     1081 gtcaccgaga tccccggtcc gaagtcgcgc gagtggttcg aacgccgcgc ggcggccgtc
     1141 ccccagggcg tgttcaacac gcaccccatc gtcacggccc gagcctccgg ggcgatcatc
     1201 gaggacgtcg acgggaaccg gctgatcgac ttcgccaccg ggatcgcggt gctgaacgtc
     1261 ggtcacacct cacctgaggt ggtcgccgcg gcgcagcgac agctcgagct cgacactcat
     1321 acctgtttcc acgtcacggc caacgagccc tacatcgagc tcgccgaacg cttgaatgcg
     1381 ctcgcccccg gcgacgcgcc caagaagacg atgttcgcga actccggcgc cgaggccgtc
     1441 gagaacgccg tgaagatcgc gcgcaaggcg acgggtcgtc aggcagtggt gacgttcgac
     1501 cacgcgttcc acggccggac gctgctggcg atgtcgctca ccgcgaaggt catgccctac
     1561 aagcagggca tgggtccgtt cgcgccggag atctaccgcc tgccgttcgc ctacccctac
     1621 cgctggccga gcgaccccga gcggtgcgcc gaggaggcgc tcgcgtacgc ccgcgacgag
     1681 atccacaagc acatcggcga ggagaacgtc gccgccctga tcctcgagcc gatccagggc
     1741 gagggcgggt tcatcgtccc ggcgccgggg ttcgtgaagg gcctcgcgga cctctgctcc
     1801 gagcacggca tcgtgttcat cgccgacgag atccagagcg gcatgggccg tgccggacgc
     1861 tggttcgcga tcgaggacga gggcgtcgtg cccgacctga tcaccagcgc gaagtcgctc
     1921 ggcggcggcc tgccgatcag cgccgtgacc ggacgagccg acctgatgga cgccgtgcac
     1981 gtcggcggtc tcggcggcac gtacggcggc aaccctgtcg cggcggcggc ggctctcgcc
     2041 gttctcgaca agatcgagcg ggagggtctg ctggaacgct ctcgaacgct cggcgagagg
     2101 gtgctcgggc ggctgcgcga gatgcaggat cggcacgacg tgatcggcga cgtgcgcggc
     2161 cgagggatga tgaccgcgat cgagctcgtg tccgatcgca agaccaaaga gccgctcggc
     2221 gggccggagg ggaacgagat cgtgaagcgc tgcctcgagg agggcgtcgt tgtcctgaag
     2281 gccgggacct acgacaacgt gatcaggctg ctgccgccaa tcacgatcga cgaaggactg
     2341 ttggacgagg gtctcgagac gctgaacgca gcactcggct cctaagactc gacctagacc
     2401 gggtcaaacg gttccgtccc aaagcattct gaattcgaag gtgactacgt agcgtcggtt
     2461 gcctcgcgat tcatccatcc gtgtcactcc cccgaacagt tcactcgaag aacggcgtga
     2521 tggcctccac cgcctgcgcc agccgcgtgc cgcgagatcc ggtgaccttc acccaaggga
     2581 gactgcgagc cgataggacc tcctcgaacc gcttggtcat cgccgcgcgc gtctcgtccc
     2641 cgagacgcag gccgtcgtct tcccaaggga catctctgtg atcggtgagc aggtacaacg
     2701 caaaggcggc accgtgcgca tcagcggcac gccaaacggc ttccgaggtt gaaccgaggt
     2761 aaacctcctc ccaaagcgcc gttgcgagcg agtccgtgtc gcagaacatg atgccgaccg
     2821 gagcctcccg cgccatacga tcttcgagcg cgttctgctc gagagcgatc cgaaggaagt
     2881 cgcttggcag ccacgtgtcc cccgttccga tcgtgaactt ctccaccgtg tattcacgac
     2941 cgtactcggg aacccagatg gtcgcgtacg cagctgcgag cgaccgcgcg agggtggtcg
     3001 accctgtcga ctcggccccg acgatgcaga cacgcttgag gtagtgagcg cgcaccggcg
     3061 catcgatgta gtcccagtat cgagccgggt ccgaccgaac ctgcgtcccc gagatctgca
     3121 cgagtccgcg accgacgaac acgttctcgc agtcgctgcc ctgctgctcg gagatcgcag
     3181 ccgcccaacg ggagcc
//
LOCUS       DASUYD010000211         6102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_144014, whole genome shotgun sequence.
ACCESSION   DASUYD010000211 DASUYD010000000
VERSION     DASUYD010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144014"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF195_11340"
     CDS             <1..218
                     /locus_tag="VF195_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018550232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581450.1"
                     /translation="VFVANTLRLSRALTEAGRPHSVVPLSGITHMTTEESVAENLLLL
                     QVRFLREALGFIDEVDAVDEVDAARPG"
     gene            254..1483
                     /locus_tag="VF195_11345"
     CDS             254..1483
                     /locus_tag="VF195_11345"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX6581451.1"
                     /translation="MPIVVQKYGGTSVDGVDRLRVVADRVVRARDAGNDVIVVVSAMG
                     QTTDELARMAKDIAPEPDPREMDMLLTAGERIAMALLGIAINERGCRAASYTGSQAGI
                     ITDTAHGQAKIIEIRPKRILEALEAGNVVIIAGFQGLSSAYEITTLGRGGSDLTAVAM
                     AAAVGAEVCEIYTDVGGVMTADPRIVPGARLIERIGYDDMLELAAAGAKVLQSRSVEL
                     ARRSGLRLHVRSSFDDEAPGTWVQEVDDDMEDVLISGVALETDEAKVTFDGIPDTPGV
                     AAALFKAVASAGVSVDMIVQNVSHEGVTDLSFTAPIADVSSLEATIGGIAQQVQARNW
                     SVDDGVAKVSLVGAGMKSQPGVAADMFDALATEGINIEMISTSPIRISCVVRKADAER
                     ALRVVHARFGLDGAAVG"
     gene            1488..2534
                     /locus_tag="VF195_11350"
     CDS             1488..2534
                     /locus_tag="VF195_11350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX6581452.1"
                     /translation="MGLPKNLKVAVVGATGAVGTEMLRILEARAFPVDELVPLASARS
                     AGRTIPFDGREVAIRELSLEALRGADLSLSAAGASIARGFVREAAAAGTVCIDKSSAF
                     RMEPDVPLVVPEVNPEALEGSPKIIAVPNCTTIVAMMALAPLHRAAGLRSLVLSSYQA
                     VSGAGRDGTRELAEQVEKLHDQVEELGRPDVDALPVGDVFGKTMAFNVLPKIDSFAED
                     GSTGEEIKTVNESRKILGIPDLDVAATCVRVPVPVGHSVSLLARFSRAITPEEARSLL
                     DGAPGVELRDDPANDVYPTPLEAAGRDEVLVGRIRQAPDHDDALLLFACGDNLRKGAA
                     LNGIQIAERLFSTG"
     gene            3054..4721
                     /locus_tag="VF195_11355"
     CDS             3054..4721
                     /locus_tag="VF195_11355"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prealbumin-like fold domain-containing protein"
                     /protein_id="HEX6581453.1"
                     /translation="MARLFDSLKRSSRLRSPVTVFAVIGAMILAIGIPAALASHPEVS
                     LAGSNFEIDTNANLKVDDAAPSIDWVGTAETRQGDQPTGANDDSFGQGTKEDTAVPTI
                     VDGGIPPNKSDLLNFGVYLETNANGKFLNLFWHRVQEPSGTTNMDFEFNKSTTISGNT
                     VTPVRSSGDLLIQYDLAQGGTNPQLFVSRWVTSGASSQCQASNTVPCWGARTNLSAAG
                     DAAGSINTTAIASGDSDGLGPVSVRTFGEAQLDFEALAGGTTGCVSFGSAYLKSRSSD
                     SFTAALKDFIAPTPLNLTTCGTVNIHKQDDAGTALAGAEFTLFTDNAPLGGSAPHGAE
                     DTATALKCTTNAAGNCSITSVPFGKYWAVETVTPSGHDSAADQYVDLSASTPNLTISL
                     TFVNPRQRGAILVTKVAKHKAAASGQIAVSGVSITVDGVTKQTDANGQACFDNLLFGS
                     YTVTETVPTGYHAGTGQNGSITVNNKASCSDTTYVGETHTITNIPLTNLTVSVTSQIT
                     GGTASRITCTGLTPDPADGTPNGFDDTSETVKDLEPGTYTCIVDIDP"
     gene            4962..5318
                     /locus_tag="VF195_11360"
     CDS             4962..5318
                     /locus_tag="VF195_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581454.1"
                     /translation="MRFTALLTGAMLTIALALGVAQAAGRTANGRCWTDPGSASIGQT
                     VEIWADGLPTKTALNIFVTDEHGTYGWGVGVQPSGTIERVWYVPDEVGTSTIRITGPE
                     RPNNVKVYATCSMAVT"
BASE COUNT         1051 a         2051 c         2023 g          977 t
ORIGIN      
        1 acgtcttcgt cgccaatacg ctgcgcctgt cccgcgcgct caccgaggcc gggcgtcccc
       61 actcggtcgt gcccctctcc ggcatcacgc acatgaccac cgaggagtcc gtcgcggaga
      121 acctgctcct gctgcaggtg cgattcctcc gtgaggcgct cggcttcatc gacgaggtcg
      181 acgcggtcga cgaggtcgac gcggcccggc cgggctgaaa ggtcgcgctc agaggcactg
      241 ctacgctcgc tccatgccca tcgtcgtgca gaaatacggc gggacctcgg tcgacggcgt
      301 cgatcggctc cgcgtcgtcg ccgaccgggt ggtgcgagcg cgcgacgccg gcaacgacgt
      361 gatcgtggtc gtgtcggcga tgggacagac caccgacgag ctcgcgcgca tggcgaagga
      421 catcgcgccg gagcccgatc cccgcgagat ggacatgctg ctcaccgcgg gggagcgcat
      481 cgcgatggcg ctccttggca tcgcgatcaa tgagcgaggg tgccgagcgg ccagctacac
      541 gggctcgcag gcggggatca tcaccgacac cgcccacggc caagccaaga tcatcgagat
      601 ccggcccaag cggatcctcg aggctctcga ggccggcaac gtggtgatca tcgccggatt
      661 ccaggggctt tcctccgcct acgagatcac caccctggga cggggcggct cggacctcac
      721 cgccgtcgcg atggcggcgg ccgtaggtgc cgaggtatgc gagatctaca cggacgtggg
      781 tggcgtgatg accgcggatc cgcggatcgt gcccggggcc cggctgatcg agcggatcgg
      841 ctacgacgac atgctcgagc tcgccgcggc gggggcgaag gtgctccagt cgcgctccgt
      901 ggagctcgcg cggaggagcg gcctccggct gcacgtgcgg tcctccttcg acgacgaggc
      961 acccggcacc tgggtgcagg aggtcgacga cgacatggaa gacgtcctga tctcgggggt
     1021 ggcactcgag accgacgagg cgaaggtgac gttcgacggg atccccgaca cgccgggcgt
     1081 cgccgccgcg ctgttcaagg cggtcgcgag cgccggcgtg agcgtcgaca tgatcgtgca
     1141 gaacgtctcg cacgagggag tcacggacct gtcgttcacc gcaccgatcg cagacgtatc
     1201 gagtctcgag gccacgatcg gcggcatcgc gcaacaggtg caggcgcgga actggtcggt
     1261 cgacgacggc gtcgccaagg tctccctcgt cggcgccggg atgaagagcc agcccggtgt
     1321 tgcggcggac atgttcgatg cgctcgccac ggaggggatc aacatcgaga tgatctccac
     1381 ctcaccgatc cggatctcgt gcgtcgtgcg gaaggcggac gcggagcgcg cgctccgcgt
     1441 ggtgcacgcg cgcttcggct tggatggagc ggcggtaggg tgaccacgtg ggtctgccga
     1501 agaacctgaa ggtcgccgtc gtcggcgcca ccggtgccgt tggcaccgag atgctgcgca
     1561 tcctggaggc ccgagcgttc cccgtggacg agctcgtccc gctcgcatcg gcgagatcgg
     1621 cgggccggac catcccgttc gacggtcgtg aggtcgcgat ccgggagctg tcgctcgagg
     1681 cgctgcgcgg cgcggatctc tcgctctccg ccgccggcgc gtcgatcgcc cgagggttcg
     1741 tccgcgaggc cgccgcggcc ggaaccgtgt gcatcgacaa gtcctccgcg ttccgcatgg
     1801 agccggacgt gccgctcgtc gtgcccgagg tgaaccctga ggcgctcgag ggatcgccca
     1861 agatcatcgc cgttcccaac tgcacgacga tcgtggcgat gatggcgctc gcgcccttgc
     1921 atcgggccgc cggtctccgc tcgttggtgc tgtcgtcgta ccaagccgtc tcgggggcgg
     1981 ggcgtgacgg gacgcgcgag ctcgccgagc aggtcgagaa gctccacgac caggtggagg
     2041 agctcggccg gccagatgtc gacgccctgc cggtcggcga cgtcttcggc aagacgatgg
     2101 ccttcaacgt cctgcccaag atcgactcct tcgccgagga cggctccacc ggagaagaga
     2161 tcaagaccgt gaacgagtcg aggaagatcc tcggcatccc cgatctcgac gtggccgcaa
     2221 cgtgcgtccg tgtcccggtt ccggtcggcc actcggtgtc cctgctcgcc cgattctccc
     2281 gagcgatcac gcccgaggaa gcccgatcgc tcctcgatgg ggcacccggg gtcgagcttc
     2341 gggacgaccc ggcgaacgac gtctatccga cgcccctgga ggctgccggt cgtgacgagg
     2401 tgctcgtggg tcgcatccgg caagcacccg atcacgacga cgcgctcctc cttttcgcct
     2461 gcggggacaa cctccggaag ggcgccgcgc tgaacggcat ccagatcgcc gagcgcctgt
     2521 tctccaccgg atgacccatc agttcgggct ccgggatccg cggtagcaca tccggccggt
     2581 accgatcccg cgagttgcct cgctccgcat gtggacaacc gtgcgatgaa gcgcaggtga
     2641 acgttctgaa ccacctgggg tttccctctc atataggcca aaggaactaa tgcgccgcgt
     2701 gcgtcttggt gctactgtcc gtcagctacg gcatctgccg tcgcccgttt gggggctcgg
     2761 gaatccccca agtgggggtg gagggaccct tcagatcacg atcggtgatc attggggtcc
     2821 ctttcttttc ccgcgaagtg gggtggaacg gccgtgggaa cagctacgcg tcctcgcgtg
     2881 ccgtggtcct cctccccacc ggaggtgcac gacggggcgg ctgccgcccg cccgcgatcc
     2941 gaaaggaggg ttccccaacc tgagagatcc gagctagtcc gccggaggtg cgccgaacgg
     3001 cactcggaca gcagcggcac atctggatca ccactccgaa ggaggagtct ttgatggcac
     3061 gtttgttcga ttcactcaag aggagttcac gactccgctc accggtcacg gtgttcgccg
     3121 tgatcggagc catgatcctc gcgatcggga tcccggccgc gctggcgagt catcccgaag
     3181 tcagtctcgc gggtagcaac ttcgagatcg acaccaacgc caacctcaag gtggacgacg
     3241 ctgcgccctc catcgactgg gtaggcaccg ccgagacccg ccagggggat cagccgacgg
     3301 gcgcgaacga cgattccttc gggcaaggca cgaaggagga caccgccgtt ccgacgatag
     3361 tggacggcgg catccccccg aacaagagcg acctgctcaa cttcggcgtc tatctcgaga
     3421 cgaacgcgaa cgggaagttc ctgaacctgt tctggcatcg cgttcaggag ccctcgggca
     3481 cgacgaacat ggacttcgag ttcaacaagt cgaccacgat ctccggcaac accgtgacgc
     3541 cggtccggag ttcgggtgac ctgttgatcc agtacgacct ggcgcagggc ggcacgaacc
     3601 cgcagctctt cgtgtcgcgg tgggtcacgt ccggcgcttc ctcgcagtgc caggcctcga
     3661 acacggtccc gtgctggggc gcgaggacga acctgagcgc tgccggcgat gcggcaggct
     3721 cgatcaacac caccgcgatc gcgagtggcg actcggacgg tctcggtccc gtgtccgtgc
     3781 gcacgttcgg agaggcgcag ctcgacttcg aggcactcgc gggcggtacg accggttgcg
     3841 tgtcgttcgg aagcgcctac ctcaagagcc gttcgtcgga ctcgttcacg gcggcgttga
     3901 aggacttcat cgctccgacg cccctgaacc tcaccacgtg cggcactgtg aacatccaca
     3961 agcaggatga cgccggtaca gcgctcgccg gtgcggagtt cacgctcttc accgacaacg
     4021 cgcctctggg aggcagcgcg ccccacggag cggaggacac cgcgaccgcg ctcaagtgca
     4081 cgacgaacgc ggccggcaac tgcagcatca ccagcgttcc gttcgggaag tactgggcgg
     4141 tggaaacggt cacgccttcg ggccatgact cagcggccga ccagtacgtc gacctgtccg
     4201 catcgacgcc gaacctgacg atcagcctca cgttcgtgaa cccgaggcag cgtggtgcga
     4261 tcctcgtcac gaaggtcgcc aagcacaagg ctgctgcgag cggtcagatc gccgtctcgg
     4321 gagtcagcat caccgtcgac ggggtgacga agcagaccga cgcgaacggg caggcgtgct
     4381 tcgacaacct gctgttcggc agctacacgg tgaccgagac cgtgccgacc ggctaccacg
     4441 ccggaacggg acagaacggg tcgatcacgg tgaacaacaa ggcgagctgc tccgacacga
     4501 cgtacgtcgg cgagacccac acgatcacca acatcccgct gacgaacctc accgtgtcgg
     4561 tgacctcgca gatcaccgga ggaacggcgt cgaggatcac gtgcacgggc ctgacgccgg
     4621 atccggccga tgggaccccc aacggcttcg acgacacgtc ggagacggtc aaggacctcg
     4681 agccgggcac gtacacctgc atcgtcgaca tcgacccgta gtcgatcgac ggttcagact
     4741 aggaacgcgg aaggaggggc cctcgggccc ctccttcccg tctggatgcc acaggcggat
     4801 ggacctccgt cctcccccgg gcgccggcct cggtaggact atcgaacccg actccctccc
     4861 tcggttgggt gctttcgctt tacctacgct cccgattcgg gcccacccag gccgatagag
     4921 aggacggacc cgccgtcgac ggcgggcgag gggtgaggct gatgaggttc acggcgttgc
     4981 tcacgggtgc gatgttgacc atcgccctcg cgctcggcgt ggcgcaggcc gcgggccgca
     5041 ccgcgaacgg gcggtgctgg accgatcccg gctcggcgtc gatcggccag acggtcgaga
     5101 tctgggctga cggtctcccg accaagaccg cgctgaacat cttcgtgacg gatgagcacg
     5161 ggacgtacgg gtggggtgtg ggcgtccagc cgtctggcac gatcgagcgc gtctggtacg
     5221 tgcccgatga ggtcggaaca tccacgatcc ggatcacggg acctgagcgt ccgaacaacg
     5281 tgaaggtcta cgcgacctgc tcgatggcgg ttacctgatc gcggcgtaag actcgcgcca
     5341 cgtcgctctg ccgggtgatg accgatccgc ggataccccg acgcacacgg atggtgttgc
     5401 cgctcgccgc ggcgggaccg gccaaggtcc gaggaacgga agcgcaggga gcgagatcct
     5461 catgaactca tcctgaccgg cacttcttgc ctcgccacac acgacgtatt gccgcatagt
     5521 ccctttcgag cgtctgccta gatcagacgt gatcaccggg atgtccccgg cgtcgcgttg
     5581 aggttgtttc tttgcatgga catccggcga ccccgaggaa ccgaacgccc acaggtcact
     5641 acctgggaca gtgcgcgcca ttcggtcatt tgggggactg cgccacgaga caactgtttt
     5701 gttactctcc gtcagcttga cgtaggttca acgggtgacg cccgttggaa ggctgggggt
     5761 gggtctttag tcggtcccaa gcccaatcag accggaaggc ttggaatgac ttcttcacgt
     5821 cgccagaggc gagggttctc tcgcgtcatc tcgcttctcg taccactcgc gatcacggtc
     5881 tcctcactcg gaggcgcagc gatcgcgatc gctgaagagc ccagcccgtc tccgacggag
     5941 acgcagtctc ccgatccgag tccaagcccg gatccgagtc caagcccgga tccgacaccg
     6001 accctcgacc cgagtccgac cgcagacccg tcgccgacgt cagagcccag cccaaccgct
     6061 gacccagcag cctcgtcagc tgccgacccc atcttcgatc ca
//
LOCUS       DASUYD010000212         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1250131, whole genome shotgun sequence.
ACCESSION   DASUYD010000212 DASUYD010000000
VERSION     DASUYD010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1250131"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF195_11365"
     CDS             <1..210
                     /locus_tag="VF195_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013422010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Ni]"
                     /protein_id="HEX6581455.1"
                     /translation="ADLVKEHLWVLWTDYFKPEHVEKYPQLHELFWNATKEAGLAKKA
                     VDPSQGQKLLDSIDEIGKIFWETKA"
     gene            307..1284
                     /locus_tag="VF195_11370"
     CDS             307..1284
                     /locus_tag="VF195_11370"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6581456.1"
                     /translation="MKDIGLVGVPFSGKSTLFTALTRTGGHGGQANHAVVPVPDPRVD
                     VLTEMESSRKTVHAQVGFVDVPGGTSSSLGIAKLREADALAIVVRCFGPDAEPVSEVA
                     EVRADLLLADQAVIEPALEKAEKRAKGKRTSEVEALAAANAALDAETPLSDAGLSEEH
                     RRELKPIAALTIKPWVVVANLEEGTDVPGGLPDGTVGVYASIEAETAEMDAAEARALL
                     DEFGVHEPGLETVIRAGYRAIDLITFLTTGDDETRAWEVRSGAKAPEAAGVIHSDLER
                     GFIRAEVISYDDLVAAGSMDAAKQQGKVRVEGKDYEVREGDILHVRFAV"
     gene            1377..1769
                     /locus_tag="VF195_11375"
     CDS             1377..1769
                     /locus_tag="VF195_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HEX6581457.1"
                     /translation="MAEESYRIGEAASMLGVRVETLRRWEREGKLTTTRTSGGQRMVA
                     AAEVARLLAEGRERPKITSASVRNRFPGTVIEVKRDKLAATVEILAGPHRILAFVTRE
                     AVDEMGLEPGMRAVATVKATNVMLEVDE"
     gene            1766..>2191
                     /gene="modA"
                     /locus_tag="VF195_11380"
     CDS             1766..>2191
                     /gene="modA"
                     /locus_tag="VF195_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX6581458.1"
                     /translation="MKKTLLLAMSSALLLAACSDPDNGGRASGGSGGTAEGVELTVSA
                     ASSLTEAFTEIGDGFESANPGSTVTFNFGPSDGVAGQINEGAPVDVFASASPTWMDSV
                     QDDGPGVTGRADFAQNRLAIIVPVDNPAGIENLNDLTEDG"
BASE COUNT          381 a          703 c          785 g          322 t
ORIGIN      
        1 gccgacctgg tcaaggagca cctgtgggtg ctgtggaccg actacttcaa gcccgagcac
       61 gtcgagaagt acccgcagct gcacgagctg ttctggaacg ccaccaagga agcgggactc
      121 gccaagaagg cggtggaccc ctcacagggc cagaagctgc tcgactcgat cgacgagatc
      181 ggcaagatct tctgggagac caaggcgtaa ccacgggttc tcgaaccacg ggagggcggg
      241 gccgtcggct ccgcccttcg tcgcgtctgg aggggttcgg ccggtcggct ccttagactt
      301 gaccgcgtga aggacatcgg tctggtcggc gtcccgttca gcgggaagtc gaccctgttc
      361 acggcactca cgcggaccgg cggccacggg ggtcaggcca atcacgcggt cgtgcccgtc
      421 cccgaccctc gcgtcgacgt cctgaccgag atggagagct cgcgaaagac cgtccacgcg
      481 caggtcgggt tcgtcgacgt cccgggcggg acgtcctcct ccctggggat cgcgaagctg
      541 cgcgaggcgg atgcgctcgc gatcgtcgtt cgatgtttcg gaccggacgc cgaaccggtg
      601 tccgaggtcg cggaggtccg ggcggatctg ctcctcgcgg accaggccgt gatcgagcct
      661 gccttggaga aggccgagaa gcgggcgaaa ggcaagcgca cgtccgaggt cgaagcgctc
      721 gccgcggcga acgccgcctt ggatgccgag accccgctct cggacgccgg gttgtcggag
      781 gagcaccgac gcgagctcaa gccgatcgcg gcgctcacga tcaagccctg ggtggtggtc
      841 gcgaacctcg aggaggggac ggacgttccg ggcgggctgc ccgacggaac cgtcggcgtc
      901 tacgcctcga tcgaggcgga gacggccgag atggacgccg cggaagctcg tgcgctcctg
      961 gacgagttcg gcgtgcacga gcccggtctc gagaccgtga tccgggccgg ctaccgggcg
     1021 atcgacctga tcacgttcct cacgaccggg gacgacgaaa cccgcgcgtg ggaggtgcgg
     1081 agcggcgcga aagctcccga ggcggccggc gtcatccact ccgatctcga acgaggcttc
     1141 atccgtgccg aggtgatctc gtacgacgac ctggtggcgg ccggctcgat ggacgcggcc
     1201 aagcagcaag ggaaggtgcg cgtcgaggga aaggactacg aggtccgcga gggagacatc
     1261 ctccacgtcc gcttcgcggt ctgaccttgg gtccgtccct agcaccaggg tcatggttcg
     1321 atatcaccag ctatctctgg cgactcctca ccgtaaccgc agtagactct cgccgcatgg
     1381 cggaggagtc gtaccggatc ggtgaggcgg cctcgatgct cggtgtgcgt gtcgagaccc
     1441 tgcgacgctg ggagcgcgag gggaagctca ccacgaccag gacgtcagga ggccagcgca
     1501 tggtcgcggc cgcagaggtg gctcgcctcc tcgccgaggg tcgcgagcgc ccgaagatca
     1561 cctccgccag cgttcggaac cggttcccgg gcacggtgat cgaggtcaag cgcgacaagc
     1621 tcgcagcgac cgtcgagatc ctcgcaggtc cacaccggat ccttgcgttc gtcacgcggg
     1681 aggcggtcga cgagatgggt ctcgaacccg ggatgcgcgc cgtcgccacg gtcaaagcga
     1741 ccaacgtgat gctggaggtg gacgaatgaa gaagacgctg ctcctcgcga tgtcctcggc
     1801 gctactcctc gcggcgtgca gcgatccgga caacggtggc cgagcgagcg gcgggagcgg
     1861 cgggacggcg gaaggggttg agctcaccgt gtcggccgca tcctcgctca cggaggcgtt
     1921 caccgagatc ggggatgggt tcgagtcggc gaatcccggt agcaccgtga ccttcaactt
     1981 cgggccctcc gacggggtcg ccgggcagat caacgaaggg gccccggtcg acgtcttcgc
     2041 gtcggcaagc cccacttgga tggattcggt acaggacgac ggcccgggcg tgacgggacg
     2101 agctgacttc gctcagaacc ggctcgcgat catcgtgccc gtggacaacc ccgccgggat
     2161 cgagaacctc aacgacctga cggaggacgg c
//
LOCUS       DASUYD010000213         2087 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_587506, whole genome shotgun sequence.
ACCESSION   DASUYD010000213 DASUYD010000000
VERSION     DASUYD010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587506"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(272..1132)
                     /locus_tag="VF195_11385"
     CDS             complement(272..1132)
                     /locus_tag="VF195_11385"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020516207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581459.1"
                     /translation="MSAVSTQPRQVRLPEPVARRPRKRWTRFVLPTYVMAFIVYLSFP
                     IFVIILFSFNQTRGGFGTSPRVKTTWLGFTTEWYGRLFELPDLTASLRHSLTIGLLSA
                     LAATALGTLLGLALARYRFRGRGGTDFLIFLSIASPEIVLGAALASFFVALGVTRGLS
                     TLFLSHTMFSIAFVAVTVRARAAGLDRSLEAAAMDLGATPVTAFFKVTLPLLFPGILA
                     GFLLAFALSMDDFVISQFTQGPATMFPTWVFGSTRVGIPPHVFAFATIIFVTGATLAL
                     ALALTQRRRA"
     gene            complement(1129..2034)
                     /locus_tag="VF195_11390"
     CDS             complement(1129..2034)
                     /locus_tag="VF195_11390"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014626847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581460.1"
                     /translation="MERPVQPALHLVDPPERRPRRSAAPYILSGPALIWLLVLFVIPM
                     FAMASLSLQEGTSLEGFRQTFRFANFTDAIADNGTFLVRSIRNGVIVTGAALLIAYPV
                     AYWIAFYGGRFKSVFLFLLLLPFFISFVIRTVAWSFILSDDGIVLGTLKSIGLLPEGY
                     HVLGTTFAVIAGITYNLVPFTILPLYVSLEQVDRSLVEAARDLYSNRAQAFLKVVLPL
                     SLPGVLAAVLLTSIPAIGDYVNAAVLGNFAPGTSMIGNVIQAEFLRNRDYPEGAALAF
                     VLMAFMVVGAVIYARVLGTERITEG"
BASE COUNT          418 a          690 c          697 g          282 t
ORIGIN      
        1 ccaggtagcc caggctcccc cacacgagga tccccccgac cagcgtcgcg gtgaccgccc
       61 aaccgatccc catgccggcc caagcgccgt cgcgtccgga acccgccatg ggcgcggaag
      121 tatatccgcg gccgattcgg cccgtcaatc ctcacggatc ccgtttccgc aggtagatgg
      181 cccttttgcc gtcgtcggtc ccgagcttgt gaaggatttc acgaactccg gggcggacgc
      241 gacgtcgcgc gcccgcgcac cggcggcgga gtcaggcccg gcggcgttgc gtgagcgcca
      301 gcgccaacgc cagcgtcgca cccgtgacga agatgatcgt cgcgaaggcg aagacgtggg
      361 gcgggatccc gaccctcgtc gagccgaaca cccaggtcgg aaacatcgtt gcgggaccct
      421 gcgtgaactg gctgatcacg aagtcgtcca tcgacagggc gaacgcgagc aggaaccccg
      481 ccaggatccc cgggaacagc aagggcagcg tcaccttgaa gaacgccgtc acgggcgtcg
      541 cgccgagatc catggccgcg gcctccaggg accggtcgag ccccgcggct ctcgcccgga
      601 ccgtcaccgc cacgaacgcg atgctgaaca tcgtgtgcga caggaacagg gttgacagcc
      661 ctctcgtgac gccgagggcg acgaagaacg atgcgagcgc agccccaagc acgatctcag
      721 gcgacgcgat gctcaggaag atcaggaaat cggtcccgcc gcgtccacgg aaacggtatc
      781 gggcgagcgc gagaccgagg agcgtcccca acgccgtggc ggcgagcgcg gacaggagcc
      841 cgatcgtcag cgaatgccgg agcgaagccg tcaggtccgg gagctcgaac agtcgcccat
      901 accactcggt ggtgaacccg agccaggtcg tcttgacacg cggcgaggtg ccgaacccgc
      961 cccgtgtctg gttgaagctg aacaggatga tgacgaagat cgggaaggac agatacacga
     1021 tgaatgccat gacgtacgtg ggcaggacga accgcgtcca acgcttcctc ggccgccgag
     1081 cgacgggctc ggggagtcga acctgccgcg gctgggtgct gacggcgctc atccctcggt
     1141 gatcctctcg gtgccgagga cccgcgcgta gatcacggcc ccgaccacca tgaaggccat
     1201 cagtacgaag gcgagcgccg ctccttcggg atagtcccgg ttccggagga actccgcctg
     1261 gatgacgttg ccgatcatgg acgtgcccgg cgcgaagttg ccgagcaccg cggcgttcac
     1321 gtaatcgccg atcgcgggga tcgatgtgag caggaccgcc gcgagcacgc ctggcagcga
     1381 cagggggagc accaccttga ggaacgcctg ggcccggttc gagtacaggt ccctggccgc
     1441 ctcgaccagg ctccgatcga cctgctccag cgagacgtac aagggcagga tcgtgaacgg
     1501 gacgaggttg tacgtgatcc cggcgatgac cgcgaacgtc gtgcccagga cgtggtagcc
     1561 ctccggcagc aacccgatgc tcttcagcgt gccgaggacg atcccgtcgt cggacaagat
     1621 gaagctccac gcgaccgttc ggatcacgaa cgagatgaag aacggcagca ggaggaggaa
     1681 caggaagacg gacttgaacc ggcccccgta gaacgcgatc caatacgcga cggggtaggc
     1741 gatcagcagc gcggcccccg tcacgatcac cccgttgcgg atcgaccgga ccaggaacgt
     1801 gccgttgtcg gcgatcgcgt cggtgaagtt cgcgaaccgg aacgtctgcc ggaaaccctc
     1861 gaggctggtt ccctcctgca gcgacaggct cgccatcgcg aacatcggga tcacgaacag
     1921 caccagcagc cagatcaacg ccggccccga caggatgtag ggagcggcgg accgtctcgg
     1981 gcgtcgctcc ggcgggtcta cgagatgaag agcgggttga acaggtcgtt ccatacctcg
     2041 atctcatccc cctcgaggac ccggtagttg tagaggttgg cctcgag
//
LOCUS       DASUYD010000214        13395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1690792, whole genome shotgun sequence.
ACCESSION   DASUYD010000214 DASUYD010000000
VERSION     DASUYD010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1690792"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1512)
                     /locus_tag="VF195_11395"
     CDS             complement(<1..1512)
                     /locus_tag="VF195_11395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX6581461.1"
                     /translation="MTGPDPSVANGLDRGPARDGLTGEEAEARFDRVGPNEIPMGPRA
                     SWRRRFGRQLLEPMSLLLLAAAAVEGFGLGERLEAVAILAIVLLNAVIGTVQEGRAER
                     ALDALRSMEPRYATVIRDARRQRIDLARVVPGDIVVLAAGDRVPADIELLVVNGLQVD
                     ESVLTGESLPVDKLVPAPVEDAERGRGFAGTVVTNGEGLGVVVATGASSSLGRIAAEL
                     GEARAPTPLQRRLRRLTKSLGLAAVGIAGGVFVLTLVRLGTGEDQVRQAFLSAVALAV
                     AAVPEGLPAVVTVVLALGVRRMAERGAIVRRLPAVETLGSSTVILTDKTGTLTENRMQ
                     VDRIVVPGETPVRAIVGSVPERLATAIALGSDATLDPPTGDPLEIALLEAVGHAQVRR
                     IRDMQRRLGSLPFDPGRRRTVTVSEGSGAAIAVLKGAPEVVLAASVDATDREGSRGPL
                     AGDTFLRFAEDLASSGSRVIAFAIREGPDIEPDRPPEEGFTFLGIVGLRDPVRA"
     gene            complement(1517..4111)
                     /locus_tag="VF195_11400"
     CDS             complement(1517..4111)
                     /locus_tag="VF195_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013601176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HEX6581462.1"
                     /translation="MQQLERREPEGLSEAAAAARLLRDGPNLLPVRRTRPLWLEAVGQ
                     LTHFFALMLWVAGVLAFVAGLPQLGVAIFVVVLANGAFAFAQEHRAQRAAERLRDLLP
                     KRATVIRGGRRFDVDAAELVIGDLVVLEAGDRISADMRIERAAAAMLGTAALTGESVP
                     VGVDAGDEALAGTFLVEGEATGIVTATGTDTRLAGIAALTTSAGGDPTPLTIELHRLV
                     RTVALIAAGVGALFLGLSLLIGISLTDAFLFAIGVVVALVPEGLLPTVTLSLAIGAQR
                     MAQRHALVRRLDAVETLGSTTFICTDKTGTLTRNEMAAVEVWTPAGTARIEGQGYEPN
                     AQVFLGPEMDEEPLRLLALVAARCSSGHADLREGMWLAIGDPMDAAVDVLARRVGVDV
                     EGDRKRRPEIERFPFDPRRRLVSLAAGDRLFVKGAPDTLLARCRHAPDSAEGAVDELT
                     ARGLRVIAVGTRPLDEVPVARDAAERDLELLGLIGLLDPPRPEVMDALTTCRRAGIRV
                     AMVTGDHPATAMAVARRVGLAGPGEMALIGDELPDDDEVLGAILDRDGIVLARVSPEQ
                     KLRIARALRSRGHVVAMTGDGVNDGPALQAADIGVAMGLSGTDVAREAADLVLLNDDF
                     GTIVGAIEQGRATFGNIRRFLTYHLTDNVAELAPFVVWALSGGRFPLALGVLQILALD
                     IGTDTLPAVALGAEPPAARTLDGPPVRGRLLDRTVAVRALGIAGPIEALLGMGAFLLS
                     MLASGWRPGDAFPTGAPLLAASGATFATVVLGQMANAFACRSATQPPWRIAWLSNPLL
                     ILAVAIELVVALGASLYWEPLAAMLDQSPPPTIGWIVAASAVTVILMADAAFKRIFRR
                     RLTSTDRP"
     gene            complement(4198..4689)
                     /locus_tag="VF195_11405"
     CDS             complement(4198..4689)
                     /locus_tag="VF195_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6581463.1"
                     /translation="MLDLWDEIAAIERRMDDVVRGFLGTRARIASPAIGQFAQKPFVP
                     RMDAFARDDDLVVKLELPGIDPEKDVSVEVREGILVITGERRHEKEIKEEAYYRMEAS
                     YGTFERQFAIPEGVDESRISATYADGVLEVVVAKGAVKEAEKAVEARKIPVLTGKKPA
                     KAA"
     gene            complement(4720..5640)
                     /locus_tag="VF195_11410"
     CDS             complement(4720..5640)
                     /locus_tag="VF195_11410"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX6581464.1"
                     /translation="MEILYATDGLPPATAAGELLRRLADPSRVNVAILFVQELGGEAM
                     DDSVAIGLEEAESAFRGAGIATRVLRMKGDAVISIEKVLADQGSGLVVLGAGNHDWLG
                     RLAFGSVSTHMLHSAPVPLLLVHRTPDPTHERLHVLFGADGSPSAESAMDTLAAMTEP
                     DRIDLEVRTAVHTPELGFSAHPGAFVPTRFIEDLFAAEEATAKKHLGETLDRARVMGF
                     TAHGSLGVGWPANDLLSHGEKKEADLIVVGARGLGTLARMTMGSVSAHVARHAPAALV
                     SHAWGYPVETEEIEEPDGDVSSNRYAVRWG"
     gene            complement(5646..6983)
                     /locus_tag="VF195_11415"
     CDS             complement(5646..6983)
                     /locus_tag="VF195_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020253563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX6581465.1"
                     /translation="MRRIVIMGAAGRDFHNFNVRYRDDPQTEVVAFTAAQIPFIADRT
                     YPMELAGPRYPGGIPIYPEEDLDTLLSERHVDGVVFAYSDVSHEYVMHRASQVLAAGA
                     DFELLGPDSTMIPTTIPVVAVCAVRTGAGKSQTTRRIARIVREEGLKPVVVRHPMRYG
                     DLLAERVQRFESIDDLDRADVTIEEREEYEHHIAEGTPVFVGVDYEAIKDEIEGGWDV
                     LLFDGGNNDLPFYRPTVHIVVADPLRAGDEATYHPGETNVRMADAVLINKVDVAKRAD
                     LHAIEASIRAVNATAQIIRAASHLEVDDPDAISGKRVLVVEDGPTLTHGGMKYGAGVV
                     AARRYGAAEIVDPRPYAEGTLGEAFDRYDVGPVLPAEGYSVEQRHELQRAIDSTPADL
                     VVIATPIDLRRVVKIEKPCVRVTYRLEELAGEPPLRSIVEPAIRAARRRQTGR"
     gene            complement(7182..8504)
                     /locus_tag="VF195_11420"
     CDS             complement(7182..8504)
                     /locus_tag="VF195_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014046025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEX6581466.1"
                     /translation="MGFRDRAEAGRSLGARLLPFRASSPVILGIPRGGLPVAAEVARA
                     LNAPVDVLVVRKLGVPWRPELGFGAIGEGGASVIDADVISMAGLGPSDTQRVIQEETA
                     ELERRIHLYRGDRAPVSVKGRTVILVDDGMATGGTVRAAIDVVRRRGADRIVVAVPVA
                     PPHTVESLRHVADEVVVLRAEEPFLAVGQFYDEFPQVSDEEVARILAGSEPVVTPAFA
                     DEPIDRTVEIDLGPVRLVGDLVIPAGATGIVVFAHGSGSSRHSPRNRSVARALNGVGL
                     ATVLLDLLTVEEELDRANVFDIDLLASRLVSTTTWLHRQPEARDLRVGYFGASTGAAA
                     ALMAAAELGAGIRAVVSRGGRPDLAAPRLADVTSPTLLIVGGNDRTVLQLNREARARL
                     RCPSELEIVPGATHLFEEPGALERVARLAAGWFTGHLVGGTTGVPGRS"
     gene            8979..9359
                     /locus_tag="VF195_11425"
     CDS             8979..9359
                     /locus_tag="VF195_11425"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6581467.1"
                     /translation="MQPLGSRTTRGVNDPRVEFVGRGRRPHPATVAYAREKVLAAARA
                     APRNVTFARVTLTFEKHPSNERPAKAEAMLDVDGRVVHAEAVARSSTEAIDLVQQRLR
                     RRLDGLRRRRPRQRKGVEGRGGQG"
     gene            9356..10549
                     /locus_tag="VF195_11430"
     CDS             9356..10549
                     /locus_tag="VF195_11430"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581468.1"
                     /translation="MRTSARQRHRTYASTGVSDLLGRGDIEVLTEPRDGPCVSLFLPT
                     HGGIGADRDRIHLKNLLAEAETGLQLRALREPQIRELLAPAADHLDHPLFWERTSRGL
                     ALFLGPTWSRSYRLPIELPSRVIVARRFQVRPLFPLLSDDGRFYVLALSQNVIRLLIG
                     TRNDVHEIDLARMPGGLRDAIRYDDPEKERLYHIVGRQGGRAPIFHGRGIGGEVDKER
                     ISRYLRLVDAGLIDVLRDERAPLVLAGPDYERAIFRGITRYPALVEEGMPGSPDRVRA
                     EALHARAWPLVEPIFQRGRQEATARYRQLEGTGRTSADLAETVRAAHRGQVDVLFVWV
                     DEERWGTFDRTTGGVMTSEVRGPGDEDLLELAAVGTFLNRGTVYAVPSHGMPDGAASA
                     AILRF"
     gene            10611..10820
                     /locus_tag="VF195_11435"
     CDS             10611..10820
                     /locus_tag="VF195_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX6581469.1"
                     /translation="MRAQVGDKLVVVGHHVGDHGREAEILEVHGADGAPPYLVRWTED
                     DHHALVFPGSDASIEHVPRHEVHTN"
     gene            complement(11073..11531)
                     /locus_tag="VF195_11440"
     CDS             complement(11073..11531)
                     /locus_tag="VF195_11440"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6581470.1"
                     /translation="MTSPAVTVAPADVLAMAARVMRENGVKRLPVTDADGDLVGIVSR
                     SDLLTTFLRPDEEIRTAIESAITEPRPSTEEGRIRVLVEDGVAKLDGRVGSKSQIPWI
                     VSIARKVDGVVDVESRLEFDIDDVGPVEPRPSPWAYSGARTGDTWRSPGS"
     gene            complement(11634..11828)
                     /locus_tag="VF195_11445"
     CDS             complement(11634..11828)
                     /locus_tag="VF195_11445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX6581471.1"
                     /translation="MKRAGDVMTRRVITVHEGTPFKDVARLIREQGVSALPVVDQAGR
                     LLRVVSEADLMLKEEHAAGR"
     gene            12067..>13395
                     /locus_tag="VF195_11450"
     CDS             12067..>13395
                     /locus_tag="VF195_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX6581472.1"
                     /translation="MAAITTPALKRELGGWATVAVVIGNMIGSGVFLLPAALAVVALE
                     YGSGSLLAWVVTGVGAMLLATVFANLGRAYPRTGGPYVYVRRAFGDFMGFQTAWGYWI
                     AAWVGNAAIATAFVGALAVFIPRLGADTTNGHVWAYVTAIGTIWILTLINAWGVKQTG
                     AVQLITTVLKFVPLALIGVIGLFFMDTGNFGEFAPNGYGTGNGMIGGITAAMALTLWA
                     FIGLESATVPAEEVKDAERSIPRATIWGTLGTAVLYAVATIAVMGILPLTVLRDSTAP
                     FADAATEIFGGTFWGKVVALVVMISTFGALNGWILLQGRVPYAAAKDGLFPRVFGKLG
                     RSGTPVFGLVASSVLITGLMALNYNKNLVDNFTFVILLATLVTLVPYAFSAAADAYLM
                     ITDRSAFVGRRLVRDIVVAAVGFAYAVWTMIGSGMEIIGQGFVLWMIGVPV"
BASE COUNT         2162 a         4740 c         4415 g         2078 t
ORIGIN      
        1 cgcccggacg gggtcgcgga gacccacgat gccgaggaac gtgaacccct cctccggcgg
       61 ccgatccggc tcgatgtcag gtccttctct gatggcgaag gcgatcacac ggcttcccga
      121 cgatgcgagg tcctccgcga accggaggaa cgtgtctccg gccagcggac ctcgggagcc
      181 ctcgcgatcg gtcgcgtcaa ccgaggccgc cagcacgacc tcgggggcac ccttgaggac
      241 ggcgatcgcc gcaccgctcc cttcgctcac cgtcaccgtc ctgcgcctgc ccggatcgaa
      301 cgggagcgag ccgagacgac gctgcatatc acggatccgt cgaacctggg catggcccac
      361 cgcttcgagg agcgcgatct cgagcgggtc gccggtgggc ggatcgagcg tggcatccga
      421 cccgagggcg atcgcggtcg cgaggcgttc gggcaccgac cccacgatcg cccgcacggg
      481 agtttccccg ggcacgacga tgcgatccac ctgcatcctg ttctcggtga gcgttccggt
      541 cttgtccgtc aggatcaccg tcgacgagcc caaggtctcg actgctggca gacgccgaac
      601 gatcgcgcct cgctcggcca tgcgtcggac gccgagggcg agcacgacgg tcaccaccgc
      661 cgggagcccc tccgggacgg ctgcgacggc caacgcgacc gcggacagga atgcctgcct
      721 gacctggtcc tctccggtcc caagccggac gagcgtgagc acgaagacgc cgccggcgat
      781 ccccaccgcc gccaggccga gcgacttcgt cagtcggcga aggcgacgct ggagcggtgt
      841 gggagcacgc gcctcaccga gctcggccgc gatccggccc aaggagctgg acgcaccggt
      901 tgcgacgacg actcccagcc cttccccgtt cgtcacgacc gtccccgcga agccgcgccc
      961 cctctcggca tcctcgaccg gagccggcac gagcttgtcc accggcaggg attcgcccgt
     1021 caggacggac tcgtcgacct ggaggccgtt cacgaccagc agctcgatgt cggctggaac
     1081 gcggtcgccg gcggccaaca ccacgatgtc gcccggcacc acccgcgcga gatcgatccg
     1141 ttgccggcgt gcgtctcgga tcaccgtggc gtagcgaggc tccatcgagc gcaacgcgtc
     1201 gagggctcgt tccgcgcgac cctcctggac cgtcccgatc accgcgttca gcaggacgat
     1261 ggccaggatc gcgacggcct cgagccgctc ccctagcccg aacccctcca ccgcggcggc
     1321 cgccaggagc aggagcgaca tcggctccag gagctggcgg ccgaagcgcc gacgccagga
     1381 cgcccgaggt cccatcggga tctcgttcgg cccgacacgg tcgaaccggg cttccgcctc
     1441 ttcgcccgtc agaccgtctc gggcgggtcc gcgatcgagt ccgttcgcga ccgagggatc
     1501 tgggcccgtc atcgcgtcac ggccgatcag tggatgtcag ccgacgacgg aagatccgct
     1561 tgaacgcggc atcagccatc aggatgacag tgacagccga cgccgcgaca atccatccga
     1621 tcgtcggcgg aggagactgg tcgagcatcg cggccagagg ttcccagtag agagatgcgc
     1681 cgagggcgac caccagctcg atcgcgacgg ccaggatcag cagggggttc gagagccaag
     1741 cgatccgcca gggaggttga gtcgcgctcc ggcacgcgaa cgcgttcgcc atctgtccga
     1801 gcaccaccgt ggcgaaggtc gcccccgacg ctgcgagaag tggggcccct gtggggaacg
     1861 cgtcgcctgg tcgccagccg gacgcgagca tcgagagcag gaaagcaccc atcccgagca
     1921 aggcctcgat cggaccggcg ataccgagag cccgtacggc gacggtgcgg tccaggagcc
     1981 ggccacgcac gggtggacca tccagcgtgc gggcagcggg cggttccgcg cccagcgcga
     2041 ccgcgggaag ggtgtcggtg ccgatatcca gcgcgaggat ctgcaagacg cccaaggcga
     2101 gggggaaacg gccgcccgag agcgcccaca cgacgaaggg cgcaagctca gcaacgttgt
     2161 ccgtcaggtg atacgtgagg aaccgtctga tgttcccgaa ggtggcccgc ccctgctcga
     2221 tggcccccac gatcgtcccg aagtcgtcgt tgaggagaac cagatcggca gcctcgcgag
     2281 cgacatcggt tcccgacagg cccatcgcga ccccgatgtc ggctgcctgc agtgccggtc
     2341 cgtcattcac gccgtcgccc gtcatcgcca ccacgtgccc gcgggaacgc agcgctcggg
     2401 cgatccggag cttctgctcg ggagacaccc gggcgaggac gatgccatct cgatcgagga
     2461 tcgcaccgag cacctcatcg tcgtcgggaa gctcgtcgcc gatcagggcc atctcgccgg
     2521 ggcccgcgag accgacacgc cgagccaccg ccatcgccgt cgccggatgg tcccccgtca
     2581 ccatggccac ccggatgccc gcccgccggc aggtcgtcag agcgtccatc acctcgggcc
     2641 tcggaggatc gagaagtcct atgagtccga ggagctcgag atctcgctcg gcggcgtcgc
     2701 gggcaaccgg gacctcgtcg aggggcctcg tacccaccgc gatcacccga agtccccgag
     2761 ccgtgagctc atccaccgcg ccctcagccg agtccggcgc gtgccggcac cgggcaagca
     2821 aggtgtctgg cgcgcctttc acgaagagtc ggtcgccggc cgcgagcgag accagccggc
     2881 ggcgaggatc gaaggggaac cgctcgatct ccgggcggcg cttgcggtcg ccctccacat
     2941 cgacgccgac ccgcctcgcg agaacatcga cggcggcatc catcggatcc ccgatcgcca
     3001 gccacatacc ctcccgcagg tccgcgtggc ccgacgagca tcgggctgcc acgagcgcga
     3061 gcagccggag cggttcctca tccatctcgg gtcccagaaa cacctgcgcg ttgggctcgt
     3121 atccctgtcc ttcgatccgc gctgtgcccg ccggcgtcca cacctcgacg gcggccatct
     3181 cgttccgggt cagcgttccc gtcttgtcgg tgcagatgaa cgtcgtcgac ccgagcgtct
     3241 cgaccgcgtc gagtcgacga accagggcat ggcgctgagc catccgttgc gcaccgatcg
     3301 cgagtgacag ggtcaccgtg gggaggaggc cttcaggaac cagcgccacg acgaccccga
     3361 tcgcgaagag gaaggcgtcg gtaagtgaga tcccgatcag gagcgagagc ccgaggaaca
     3421 gggcgccaac acctgctgcg atcaacgcga ccgtacggac gagccgatgc agctcgatcg
     3481 tgaggggagt cggatcgcct ccggctgagg tggtgagcgc cgcgatcccg gccagcctcg
     3541 tgtcggtacc ggtcgcggtg acgatgcccg tcgcctcccc ttccaccagg aacgttcccg
     3601 caagtgcctc atcacctgca tcgactccca cggggacact ttcgcccgtc agggccgctg
     3661 tcccaagcat cgcggcggcc gccctctcta tccgcatgtc ggcggagatg cgatcacccg
     3721 cctccaggac gaccagatca ccgatcacca gctcggcggc gtcgacatcg aagcgccgac
     3781 cgccacggat cacggtcgcc cgtttcggaa gcaggtctcg gagccgctcc gccgcgcgct
     3841 gggcgcggtg ctcctgcgcg aaggcgaacg cgccgttggc gagcacgacg acgaagatcg
     3901 ccacgccgag ctgcgggagt ccggccacga acgcgagaac accagcgacc cagagcatca
     3961 gtgcgaagaa gtgggtcagc tgcccgacag cttcgagcca caacggccga gtgcgtcgca
     4021 ccgggaggag gttcgggccg tcccgaagca ggcgggccgc ggcggcggcc tcgctcaacc
     4081 cctcgggctc acgacgttcg agctgctgca cggccgtcgc taccctagcg gggagacggc
     4141 cgaggttcct ccggaacctc ggccgccgga tcccctggcg gttcgcctag atgcgtctca
     4201 ggcagccttg gcaggcttct tcccggtcag caccgggatc ttgcgagcct cgacagcctt
     4261 ctccgcctcc ttcaccgcgc ccttcgcaac gaccacctcg agcacgccgt cggcgtacgt
     4321 cgcggagatg cggctctcgt cgaccccctc ggggatcgcg aactgccgct cgaaggttcc
     4381 gtaggaggcc tccatgcgat agtaggcctc ctccttgatc tccttctcgt gccgacgctc
     4441 gcccgtgatg acgagaatcc cttctcgcac ctcgacggaa acgtccttct ccgggtcgat
     4501 cccggggagc tcgagcttga cgacgaggtc gtcgtcgcgc gcgaaggcat ccatccgtgg
     4561 cacgaagggt ttctgagcga actgcccgat cgccggggaa gcgatgcgcg ccctggttcc
     4621 gaggaacccc cggaccacat cgtccatccg ccgctcgatc gctgcgatct catcccagag
     4681 atccagcatc ttcttcacct cccttccctc aacaggtcgt caaccccatc gcacggcata
     4741 ccgattgctg ctcacgtcac cgtcgggctc ctcgatctcc tcggtctcga cggggtaacc
     4801 ccaggcgtgg gaaacgaggg ccgccggcgc atgcctcgcg acgtgggcac tcacagagcc
     4861 catcgtcatc cgtgccaagg tcccgagtcc tcgagctccg accacgatca ggtccgcctc
     4921 cttcttctcc ccgtgggaga ggaggtcgtt cgcgggccag cccacgccga gactgccgtg
     4981 ggcggtgaac cccatcacac gcgctcgatc gagcgtctca cccaggtgct tcttcgcggt
     5041 ggcctcctcg gccgcgaaga ggtcctcgat gaacctggtc gggacgaacg cgccgggatg
     5101 agccgagaac cccagctcgg gcgtatggac ggccgtccgg acctcgagat cgatacggtc
     5161 cggctccgtc atcgccgcca gcgtgtccat cgcgctctca gccgacggcg agccatcggc
     5221 gccgaagagc acgtggagcc gctcgtgcgt ggggtccggt gtacggtgga ccaagagaag
     5281 gggcaccgga gccgagtgca gcatatgggt gctcacgctg ccgaacgcga gtcgccccag
     5341 ccagtcgtga ttcccagccc ccaggacgac cagtccggat ccctgatccg cgagcacctt
     5401 ctcgatgctg atgacggcat cccccttcat ccggagcaca cgggtcgcga tccccgcgcc
     5461 tcggaacgcg ctctcagctt cctccagccc gatcgcgacc gagtcatcca tcgcctcgcc
     5521 ccccagttcc tggacgaaca ggatcgcgac gttcacccgg ctcggatcgg ccagtcggcg
     5581 caaaagctca cccgcggccg tggccggggg cagtccatcc gtcgcataga ggatctccat
     5641 cgtgctcacc tcccggtctg ccgccgcctc gcggcccgga tcgccggttc gacgatcgag
     5701 cgcagcggcg gttcacctgc gagctcctcg agccggtacg tcacccgcac gcacggcttc
     5761 tcgatcttga cgacacgccg gagatcgatc ggcgtcgcga tcacgaccag atccgccggc
     5821 gtggagtcga tggcccgctg gagctcatgg cgctgctcga cggagtaccc ctctgccggc
     5881 aacaccggtc cgacgtcgta ccgatcgaac gcttcgccga gggtgccttc cgcgtaggga
     5941 cgagggtcga cgatctccgc ggcgccgtat cggcgagctg cgacgacacc ggcgccgtac
     6001 ttcataccgc cgtgcgtcag cgtgggaccg tcctcgacca cgaggacccg cttgccgctg
     6061 atcgcgtccg ggtcgtccac ctccaggtgg ctcgcggccc ggatgatctg tgcggtggcg
     6121 ttcacggcgc ggatgctggc ctcgatggcg tgcagatccg cacgcttcgc cacatcgacc
     6181 ttgttgatca ggaccgcatc ggccatccgg acgttcgtct cacccgggtg atacgtggcc
     6241 tcgtcgcccg cccggagtgg gtcggccacc acgatgtgga cggtcggccg atagaaggga
     6301 aggtcgttgt tgccgccgtc gaagaggagc acgtcccacc cgccctcgat ctcgtccttg
     6361 atggcctcgt agtcgacgcc aacgaatacc ggcgtccctt ccgcgatgtg gtgttcgtac
     6421 tcctcccgct cttcgatcgt gacgtccgcc cggtcgaggt cgtcgatcga ctcgaaccgc
     6481 tgcacacgct cggccaggag atcgccgtac cgcatcggat gacgcacgac gaccggcttg
     6541 agtccctcct cccggacgat gcgcgcgatc cgcctcgtcg tctgactctt cccggccccc
     6601 gtccgaacgg cgcagaccgc gacgaccggg atcgtcgtcg ggatcatggt ggaatctgga
     6661 ccgaggagct cgaaatcggc gcccgcggcg agtacctggc tcgctctgtg catcacgtac
     6721 tcgtgggaca cgtccgaata ggcgaacacg acgccgtcga cgtgccgctc ggacagcagg
     6781 gtatccagat cctcctcggg gtagatgggg atcccacccg ggtagcgcgg gccggccagt
     6841 tccatcggat acgtgcgatc cgcgatgaac gggatctgcg ccgcggtgaa cgcgaccacc
     6901 tcagtctgcg gatcgtcccg gtaccgaacg ttgaagttgt ggaagtccct cccggcagct
     6961 cccatgatca cgatgcgtct catcgtcggc cgcctcctcg tggttcctcc tggaaggctc
     7021 gcaccgaaag ggacaggctg aggggggtcc cccagccctc ctatcgcggg accttggaca
     7081 gccagggctc catggtcaac gacatcggat cgcgagtgat gcggtgcggc tgtgagagat
     7141 cggtacacgt ccggtcgagt caccgcgcga gggcatctcg ctcagctcct cccgggaaca
     7201 cccgtggtac cccccacgag gtggccggtg aaccatccgg cggcgagccg agccacgcgt
     7261 tcgagcgctc ccggttcctc gaacaggtgc gtcgctccgg gcacgatctc caactcgctg
     7321 gggcagcgga gcctcgctcg cgcctcgcga ttcagctgaa ggaccgtccg gtcgttaccg
     7381 cccacgatca agagcgtcgg cgacgtcacg tcggcgagcc tgggcgcagc gaggtcgggc
     7441 ctgccgccac gggagacgac cgctcggatc cctgctccga gctccgcggc ggccatcaag
     7501 gccgcggccg ctccggtgct cgccccgaaa tacccgaccc tcaggtcgcg cgcttccggt
     7561 tgccgatgca accacgtggt cgtgctcacg aggcggctcg cgaggagatc gatgtcgaac
     7621 acgttcgcgc ggtcgagttc ttcctcgacc gtcaggaggt cgagcaggac cgtcgcgagc
     7681 ccgaccccgt tcagcgcccg cgcgaccgac cgattcctcg ggctgtgacg actgctcccg
     7741 ctgccgtgcg cgaagacgac gatgccggtc gcgccggcgg ggatcacgag atccccgacg
     7801 aggcggacgg gtccgaggtc gatctcgacc gttcgatcga tcggttcgtc cgcgaacgcg
     7861 ggcgtgacga ccggctctga ccccgcgagg atccgggcga cctcctcatc ggagacttgc
     7921 gggaactcgt catagaactg ccccacggcc aggaacggtt cctccgcccg gagcaccacg
     7981 acctcgtccg cgacgtgccg gagggattcg accgtgtgcg gcggagccac cgggacagcc
     8041 accacgatcc gatctgctcc cctacgccgg accacgtcga tcgctgcgcg cacggtccct
     8101 ccggtcgcca tcccgtcgtc gacgaggatc actgtgcgac ccttcaccga tacgggcgcc
     8161 cggtccccgc ggtagagatg gatccgccgt tcgagctcgg cggtctcctc ttggatcacg
     8221 cgttgcgtat ccgacggacc gaggccggcc atgctgatga cgtcggcgtc gatcacgctc
     8281 gcaccgccct cccctatagc cccgaacccg agctcgggac gccagggaac gcccagcttg
     8341 cgcacgacca gcacgtcgac gggggcgttc agtgcccgag cgacctcggc cgcgacgggc
     8401 agcccgcccc tcgggatccc gaggatcacc ggcgagctcg ctcggaacgg gaggagacgc
     8461 gccccgaggc tgcgcccggc ttcggcgcgg tcgcggaacc ccagcgtcgg gccggatccg
     8521 atcaggacgc gaggaaggtc ggtccggtat cggctcgcct gcccggatcg agagctccct
     8581 cgcatcactc ggggccaccc cccgcgctca gcccaccggg atccggacgc catcgctcgc
     8641 gagcccgcgt cgccctcgtg cgccgcacgt tggaggcccg cagatcttcg tcatcccagg
     8701 tcagatcgac tcgaacccgc gtccctgccc gccgggcgtc catcgccact gatccaaccc
     8761 ctaccgggat ctcgtcaccc aaggagacca aacgtccctg ggcggtcttc ggcatgtaca
     8821 gcaccctccc ctttcggcat ggtccacgca cctccctcgt gcgaccctgt cgcgcgaagg
     8881 atccctcgcc cagggccgaa ggaccctgcc gtccggggcc ctcccgacct gtgtccgggc
     8941 ccgcgtcggt gacaggttgt tgccaggagg gaacgacgat gcagccgctc ggttcgcgaa
     9001 ccacgcgcgg cgtgaacgac ccgcgtgtcg agttcgtcgg ccgaggacgg cgtccccatc
     9061 cggccacggt tgcgtacgcc cgggagaagg tcttggccgc cgcccgggcg gcgccacgga
     9121 acgtgacgtt cgcgcgcgtc acgctgacgt tcgagaagca tccgtcgaac gagcgacccg
     9181 cgaaggccga agcgatgctg gatgtcgacg gccgggtcgt ccacgcggaa gcggtcgcac
     9241 gatcgtcgac ggaagcgatc gatctcgtcc agcagcgcct tcggcggcgt ctcgacgggc
     9301 tccgccggag acgccctcgg cagaggaagg gcgtggaggg gcgaggagga cagggatgag
     9361 gacgtccgcc aggcaacgac accgcacgta tgcgagtacc ggcgtctccg acctgctcgg
     9421 gagaggtgac atcgaggtcc tcacggaacc ccgcgacggc ccctgcgtgt cgctgttcct
     9481 gcccacacat ggcgggatcg gcgccgatcg ggatcggatc catctcaaga acctgctcgc
     9541 agaagcggaa acgggcctcc agctgcgtgc cctccgcgag ccacagatcc gcgagctgct
     9601 ggcccctgca gcggaccacc tggatcatcc gctgttctgg gaacgcacgt ccagaggact
     9661 cgcgctcttc ctcgggccca cgtggtcgcg cagctaccgg ctgccgatcg agcttccgag
     9721 ccgcgtgatc gtggcacggc gcttccaggt ccggcctctg ttcccgctgc tctcggacga
     9781 cggacgcttc tacgtgctcg ccctcagcca gaacgtcatc cggctgctga tcgggacgcg
     9841 caacgacgtt cacgaaatcg atctcgcgag gatgcccgga ggactccggg acgccattcg
     9901 gtacgacgat ccggagaagg agcggctgta ccacatcgtc ggtcgacaag gaggtcgggc
     9961 gccgatcttc cacggtcgcg ggatcggtgg cgaggtcgac aaggagcgga tctcccggta
    10021 tctgcgtctc gtggacgccg gtttgatcga cgtgcttcgc gacgagcgag ccccgctcgt
    10081 tctcgctggt ccggattacg agcgtgccat cttccgaggg atcacgcggt acccggccct
    10141 ggtcgaggaa ggcatgcctg gaagcccgga tcgcgttcgc gcggaggcgc tccacgcgcg
    10201 ggcttggcca ctggtcgagc cgatcttcca gcgaggccgt caggaggcga cagcgcggta
    10261 tcggcagctc gaaggcacgg ggagaaccag cgccgacctc gcggagacgg ttcgagcagc
    10321 ccatcgtgga caagtcgacg tgctgttcgt ctgggtggac gaggagaggt gggggacgtt
    10381 cgaccgcacg acgggcggag tcatgacgag tgaggtacgc ggtcccgggg acgaggatct
    10441 cctcgaactc gcggccgtgg ggaccttcct gaaccgtggc accgtctatg cggttccttc
    10501 ccacggcatg cccgacgggg cggcgtccgc cgcgatcctc cggttctgag gagaggtgga
    10561 gatgaacgat cgccagtgta cgccgatgac gaggatgaaa ggggaaactg atgagagcgc
    10621 aagtgggtga caagctggtc gtcgtggggc accacgttgg cgatcacggc cgcgaggccg
    10681 agatcctcga ggtccacggc gcggacgggg cgcctccgta cctggttcgc tggaccgaag
    10741 acgatcatca tgcactggtg ttcccgggtt cggatgcgag catcgagcac gttccgcgac
    10801 acgaggtcca cacgaactag ccggcgcggg agcggggaag acgatcgcgg aacgcactcc
    10861 cggtcggctt cggagtcaga tccagccgag tcgattcgac ctcgagccat ccaaggacgt
    10921 tcattcggac gtgcgaatga acgggaggca cgaagaaacg accctgtcga ccatctcccg
    10981 atcaggagta tccacgcgcg ccatgttcgg tacctcctcg atgggatcga ggatctcagc
    11041 cggtcgcccg gatccaaacc ccggaccgcg agtcacgacc cgggagagcg ccatgtgtcg
    11101 ccggtacgtg ctcctgagta agcccacggg gagggtcgcg gttccacggg accgacgtcg
    11161 tcgatgtcga actccagtcg agactccacg tccacgacgc cgtcgacctt gcgagcgatg
    11221 ctcacgatcc acgggatctg actcttggag ccgacgcgcc catcgagctt cgcgaccccg
    11281 tcttccacga ggacgcggat ccgtccctcc tcggtcgatg gccgcggttc ggtgatggca
    11341 ctctcgatcg cggtgcggat ctcctcgtcg ggccggagga aggtcgtcag cagatcggag
    11401 cgactgacga tccccaccag atcgccgtcg gcgtccgtca cggggagacg cttcacaccg
    11461 ttctcgcgca tgacgcgcgc cgccatcgcg agcacatccg ctggggccac cgtgaccgcc
    11521 ggcgatgtca tcagatcctt cgcgacgacc ccgcgcgcct tgctgcgccg gatccaggga
    11581 ggcgcttcgt tccggcggaa agtctctccc ctccagggca acccgcctgt gacctatcgt
    11641 ccggcggcat gctcctcctt caacatcaga tcagcttcgg agacgaccct gaggaggcgc
    11701 ccagcctggt cgaccacagg aagcgcactc acgccttgct cccggatgag ccgggccacg
    11761 tccttgaagg gggttccttc gtggaccgtg atcacccgcc tggtcatgac gtcgccagcg
    11821 cgcttcatga tgcccctcct ccttcacgta cctctcgaga cccagtctca taccgtcggt
    11881 ggtccctgcc ctgggccgtt cggctcggca tcggcgggtc ccgtgggctg cgcgccgcgc
    11941 acgccggcgc cacgaagtcc catggttcgg agggccggag acctaccgat gtcggatggc
    12001 gcgaggctac gttggcctcg acggaggacg atcgtcgccc tccccgaccg caaggagggg
    12061 atgaagatgg cggccatcac aacccccgct ctcaagcgcg agcttggcgg gtgggccacg
    12121 gtcgccgtgg tcatcggcaa catgatcgga tccggtgtgt tcctgctccc ggcggcactc
    12181 gccgtcgtgg cgctcgagta cggctccggg tcactgctcg catgggtcgt gaccggcgtc
    12241 ggtgcgatgc tcctcgccac ggtgttcgcg aacctgggca gggcgtatcc gcgcaccggg
    12301 ggcccgtacg tctacgtgcg tcgagcgttc ggcgacttca tggggttcca gaccgcatgg
    12361 ggctactgga tcgccgcctg ggtcgggaac gccgcgatcg ccacggcgtt cgtcggagcg
    12421 ctcgcggtct tcatcccgcg actgggggct gacacgacga acggccacgt gtgggcgtac
    12481 gtcaccgcga tcgggacgat ctggatcctc acgctcatca acgcgtgggg tgtgaagcag
    12541 accggagcgg tgcagctgat caccacggtc ctgaagttcg tgcccctggc gctgatcggc
    12601 gtcatcggcc tgttcttcat ggacacgggc aacttcgggg agttcgcccc gaacggatac
    12661 ggcacgggga acgggatgat cggtggcatc acggccgcga tggcgctcac gttgtgggcg
    12721 ttcatcggcc tcgagtcggc gacggtgccg gccgaggagg tcaaggatgc cgagcggtcg
    12781 atcccccgcg ccacgatctg gggaacgctg gggacggcgg tcctctacgc ggtggcgacg
    12841 atcgcggtca tgggcatcct gccactgacc gtgctccgtg actccacggc gccattcgcc
    12901 gacgcggcca ccgagatctt cggcggaacg ttctggggga aggtcgtcgc cctcgtggtg
    12961 atgatctcga ccttcggcgc gctgaacggc tggatcctcc tgcaggggag ggtcccgtac
    13021 gcggcagcca aggacggtct gttccccagg gtgttcggga agctcggacg gagcggcacg
    13081 ccggtcttcg gcctcgtcgc atcgtccgtc ctgatcaccg ggctgatggc cctgaactac
    13141 aacaagaacc tggtggacaa cttcacgttc gtgatcctgt tggccaccct ggtcacgctc
    13201 gtgccgtacg ccttctcggc ggcggccgat gcgtacctga tgatcaccga ccgctccgcg
    13261 ttcgtcggcc ggcggctcgt ccgcgacatc gtcgtggccg ccgtcggatt cgcctacgcg
    13321 gtgtggacga tgatcggttc gggcatggag atcatcggac agggcttcgt gctctggatg
    13381 atcggcgtgc cggtg
//
LOCUS       DASUYD010000215         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_147254, whole genome shotgun sequence.
ACCESSION   DASUYD010000215 DASUYD010000000
VERSION     DASUYD010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147254"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF195_11455"
     CDS             <1..762
                     /locus_tag="VF195_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX6581473.1"
                     /translation="HEIDLRYTDALTMADNVMTYRLTVKEVAQEFGIYATFMPKPVQG
                     VNGSGMHTHQSLFTGDRNAFFGEGDEYHLSTLGKHYIAGVLSAAREITLVTNQWVNSY
                     KRLVPGYEAPVYVCWARRNRSALVRVPQYKPGNEQATRIEYRSPDPACNPYLAFASML
                     GAGLAGIEQELELPPEASNNIYEMTEEERRAAGIRSLPADLHEAIELASGSKVLREVL
                     GDHVVEFLLRNKREEWDAYKSYVTPYEIERYLPIL"
     gene            976..2376
                     /locus_tag="VF195_11460"
     CDS             976..2376
                     /locus_tag="VF195_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581474.1"
                     /translation="MKQHVRSTLFRSPRSKLLAAGASFAAIALFALILPASVASGQTS
                     ASTSTLQNGGVLRLRLGAQDSFRFQVPDPSLADHYIDPSPASTQAVGVSTGCKLSPTS
                     GSLAQFSTQPSNADTGFVGDAIGVRVAGEGNGQPCGRIDAPAQALGLKLGTGLAGKAI
                     DYAEIDIEGKFSANFRIDGYFVTGACPPPTGTPTVTTDTYNTAGSDSGPDSGDGDNYR
                     IRFPQSNNPVGAYTAVNCLVFTPITGAASLEGGSDGTTACDATDVTECGAPVNFSLGE
                     KIDDTAQTNTSTTDSLFHLINVDGVLDCNANTFLTQTNAGITTDITRLNDGEGNNPDG
                     STCIPIPVDQDSSTTTCGAGFRQCIFLQKDLLDPNAQFEWTVTWAAEPGFYMESETQF
                     DFGNGNGLNDLLLCQADGLAGNGFDGNPDLPANDDPWCVKDTSTALQISAVDGQTYDQ
                     VTETYYGLGDPLGKRG"
     gene            complement(2327..>2881)
                     /locus_tag="VF195_11465"
     CDS             complement(2327..>2881)
                     /locus_tag="VF195_11465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581475.1"
                     /translation="ELIAADCSVLGRLASRAGRHDEAAELLEEALGEADRSDGQLLAI
                     GVIGFLAEDMVRLGDADAAVDHLARVEARAKLLGGPGIYEPLIERVRGCAFLLRSELD
                     QAEEAFDRSLAAARAAGSEFEVLLTLDARSALSQARGEERSATEAAEATSIADRLGIV
                     SVPSMLPAAIPASRAGRPSRNTSQ"
BASE COUNT          529 a          987 c          908 g          457 t
ORIGIN      
        1 cacgagatcg acctgcggta cacggacgcg ctcacgatgg cggacaacgt gatgacctac
       61 cggctgacgg tgaaggaggt cgcgcaggag ttcgggatct acgccacgtt catgccgaag
      121 cccgtgcagg gcgtgaatgg cagcggcatg cacacgcacc agtcgctgtt caccggcgac
      181 cggaacgctt tcttcggcga gggcgacgag taccacctgt cgacgctcgg aaagcactac
      241 atcgccgggg tcctctcggc cgcgcgcgag atcacgctcg tcacgaacca gtgggtaaac
      301 tcgtacaagc ggctcgtgcc cggttacgag gcgcccgtct acgtgtgctg ggcccggcgg
      361 aaccggtccg cgctcgtccg ggttccgcag tacaagccgg gcaacgagca ggccacgcgg
      421 atcgagtatc gctcgcccga tcccgcgtgc aacccgtacc tcgcgttcgc ctcgatgctc
      481 ggcgcgggcc tcgccggtat cgagcaggag ctcgagctgc cgcccgaggc ctcgaacaac
      541 atctatgaga tgaccgagga ggagcgacgc gccgcaggga tccgctcgct ccccgccgac
      601 ctgcacgagg cgatcgagct cgcgagcggt tcgaaggtcc tgcgtgaggt gctcggggac
      661 cacgtagtgg agttcctgct ccgcaacaag cgggaggagt gggacgcgta caagtcctac
      721 gtcacccctt acgagatcga gcggtatctg cccatcctct agggacctat cctctcggcc
      781 catcctctcg atctggggac tctgggccct cgaggtcctg cctctgtccc tcgttcggcc
      841 gacattccgt gcctggctcg tgcctccgat cgtatcggtc gttcgggtta cgccggttgc
      901 aacatcggcc gacacctcgt accgtatcct ccccagcgcg accgctgggt tcggccgaga
      961 ggaagggtaa ggcccatgaa acagcacgtc cgatcgacgc tattccgatc gcccaggtcg
     1021 aagctcctcg cagctggggc cagcttcgcc gcgatcgctc tgttcgcact gatcctgccg
     1081 gcatccgtgg cgagcggcca gacgtcggct tcgaccagca cgctgcagaa cggcggcgtg
     1141 ttgcgcctgc gtctcggcgc ccaagacagc ttcaggttcc aggtgcccga cccgagtctt
     1201 gcggatcact acatcgaccc ttcccctgcg tcgacgcaag cggtcggcgt ctccaccggc
     1261 tgcaaattgt cgcccacgag cgggtccctc gcccagttct cgacgcagcc gagcaacgcc
     1321 gacaccgggt tcgtgggcga cgcgatcggg gtccgcgtcg caggcgaagg caacggccag
     1381 ccgtgcggcc gcatcgacgc cccggcccag gcgctcggcc tcaagttggg aaccggtctc
     1441 gccggcaaag cgattgacta cgcggagatc gacatcgagg gcaagttcag cgcgaacttc
     1501 cgaatcgacg ggtacttcgt aaccggagca tgtcctccgc ctacgggtac gcccacggtg
     1561 accaccgata cctacaacac tgcggggtca gactcgggcc ctgactcagg agatggcgat
     1621 aactaccgca tcaggttccc gcagagcaac aaccctgtag gcgcgtacac ggctgtcaat
     1681 tgcctcgtgt tcaccccgat cacgggggct gcgtcgctcg aaggaggttc cgacgggacg
     1741 actgcatgcg acgcgacgga cgtgaccgaa tgcggagctc ccgtgaactt cagcctcggt
     1801 gagaagatcg acgacaccgc gcagaccaac acgagtacga cggactcgct gttccacctg
     1861 atcaacgtcg acggcgtgct cgactgcaac gcgaacacct tcttgaccca aacgaatgcg
     1921 ggcatcacca ccgacatcac gcggctcaac gacggagagg gcaataaccc ggacgggagc
     1981 acgtgcatcc cgatcccggt cgaccaggac tcgagcacca cgacgtgtgg tgctgggttc
     2041 cggcaatgca tcttcctcca gaaggatctg ctggacccga acgcccagtt cgaatggacg
     2101 gtcacatggg ctgctgagcc gggcttctac atggagtccg aaacacagtt cgatttcggt
     2161 aacggtaacg gtctcaacga cctcctgctc tgtcaggcgg acggactcgc tggtaacggg
     2221 ttcgacggga accccgacct gccagcgaac gacgatccct ggtgcgtcaa ggacacgtcg
     2281 acggcgctcc aaatcagcgc cgtggatgga cagacctacg accaggtcac tgagacgtat
     2341 tacggcttgg gcgacccgct cgggaagcgg ggatagcagc cggcagcatc gacggaacgg
     2401 agacgatccc gagtcgatcg gcgatcgagg tggcttcggc cgcttcggtc gccgatcgct
     2461 cttctccacg agcctgtgag agggcgctcc tcgcgtccag ggtgaggagc acctcgaact
     2521 ccgagcccgc agcccgggcc gcggccaggc tccggtcgaa cgcttcctcc gcctggtcga
     2581 gctccgatcg caggaggaac gcgcaccccc tgacacgctc gatcagcggc tcgtagatcc
     2641 ccggcccgcc caagagcttc gcgcgtgcct cgacgcgagc gagatgatcc acggcagcat
     2701 cggcgtcgcc gagccgcacc atgtcctcgg cgaggaaccc gatcacgccg atcgcgagga
     2761 gctgaccgtc ggatcgatcg gcctcgccga gagcctcctc gagcagctcg gcagcctcgt
     2821 cgtgccggcc cgcccgcgac gcgagccggc cgaggacgct gcagtcggcc gcgatcagct
     2881 c
//
LOCUS       DASUYD010000216        12780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1253826, whole genome shotgun sequence.
ACCESSION   DASUYD010000216 DASUYD010000000
VERSION     DASUYD010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12780
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253826"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /gene="kdpA"
                     /locus_tag="VF195_11470"
     CDS             complement(<1..1406)
                     /gene="kdpA"
                     /locus_tag="VF195_11470"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013110132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX6581476.1"
                     /translation="MVTIVVYIVLLIAVTPSLGAFMYRVYRREEIGRLEGIVYRLIGV
                     NPTLEQSWRRYATSCLWFSAISMVVLYVVFRVQGDLPLNPVGLGAVDQYVSFNTASSF
                     TTNTNWQAYGGETTMSYLSQMLALTSQNFVSAAAGMAVLVALFRGFTRSKREEVGNFW
                     RDLVRGTVYILLPISALVAVILVTQGVVQTLGANEAATGIQGFEQTIARGPAAGQIAI
                     KQLGTNGGGFFNVNSAHPFEGGGPLGNFIELFAILAIPAALTYTFGKWVGNVRQGWAI
                     FAVMMVLFFGGLALTVPQEHGSSQAMAAAGVPTSAQNLEGKELRIGVDESSLWAVATT
                     AASNGSVNSMHDSYRPLGALAPMFNMAIGEVIWGGVGSGLYGMIFYAIIAVFIGGLMV
                     GRTPEYLGKKIGAREVKISAIAVLVPFVLALAVIGVGVVIPGGLDGRLNAGPHGFSEI
                     FYAFISQGNNNGSAFAGYT"
     gene            complement(1408..1497)
                     /gene="kdpF"
                     /locus_tag="VF195_11475"
     CDS             complement(1408..1497)
                     /gene="kdpF"
                     /locus_tag="VF195_11475"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX6581477.1"
                     /translation="MSLADVLELILAVALLVYVTYALIRPEKF"
     gene            1740..3254
                     /locus_tag="VF195_11480"
     CDS             1740..3254
                     /locus_tag="VF195_11480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581478.1"
                     /translation="MSEQIDRSSTSQVLRLMRPPRPRRQAVAYLVAVLGTFALAASLV
                     PFRDSIDPLAKGFGFLVVVVAAAGIGGIGPGVVASMLGFIVFNFLFLPPYNTFVIGKP
                     EYVVVLFVNLGISVLISILLGRATERAAAAEAREAELRTLQDLSRELVVRAPGTEAYR
                     EIIRDILDTFGYEAGALFVRDSARSGGLREEVSVGCSPGDIVPEWNPRSTERPPERLP
                     LSIGPNNVGLLVLRGARPAPSEAESRILRALGDQLALVLERDRLLRTATDAEVYRQTE
                     RLRQTLLAAVSHDLRSPLAAIKASVSDLLEEGVTRSPDQQREALEAIDSESDRLNALI
                     ANLLDMSRIEAGVLRARVEAVDLGDAVATSARNVSRVWPDLTVRDRVEDGVMVRADPV
                     FLDRVLTNLLDNAARSAKAAAASDVEVTSKQTDGRVTIRVIDHGNGIPAEAREVLFHP
                     FYELDQRNPRLGAGLGLAIAKGFAVAMDGEIWIEETPGGGATFAVAVPVSPASP"
     gene            3251..3964
                     /locus_tag="VF195_11485"
     CDS             3251..3964
                     /locus_tag="VF195_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581479.1"
                     /translation="MSTILVVDDEEQILRALRRALNARRYEVVTAADGEDALVEAEAS
                     MPDLVVLDLNLPGIDGIEVCRRLRSWTQVPILVLSVREDERAKVQALDLGADDYLTKP
                     FGIEELLARVRALLRRSQSEEGQASRFEAGGVLIDLAARRVVRDGTDVHLTRTEWTLL
                     LAMAEHPGKLLTHRWLLERVWSTRYGEDVEVLRVFISQLRKKVEPNPSEPSMIITDPG
                     VGYRWAAQAKGEDNSRSEG"
     gene            complement(3966..4640)
                     /locus_tag="VF195_11490"
     CDS             complement(3966..4640)
                     /locus_tag="VF195_11490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6581480.1"
                     /translation="MRVVISGAGAVGRHLASDLAKRGHSVTLIEQDPAVLEIAKEWAP
                     QVDHMIGDACEPWVLEKAQLNTADVVVASTGDDEDNLVTALLAKQEFGVPKVLARVNH
                     PDNEWLFTEQWGVDAAVSPPHILTAMVEEEVTVGDLVRLLTLERGGISIVELTIPADS
                     PSAGRPLYEMRLPPDATIVAIIRESHVVIPQPETVITDGDEVLALTVPEAESALRAAV
                     LGEEPA"
     gene            complement(4642..5301)
                     /locus_tag="VF195_11495"
     CDS             complement(4642..5301)
                     /locus_tag="VF195_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEX6581481.1"
                     /translation="MRVVIMGCGRVGILLTQELSKAGHEITVIDKNASAFARLPPGFE
                     ARTIVGLGFDRDVLEEAGIKEADAFLAVSSGDNSNIVSARVAREHYHVPKVIARIYDP
                     MRADIYERLNIPTVSTTRWGVKQMILMLTHPGEEIKETLAAGDLFRMRVEIPAHLVGK
                     PVSSLNLEGKILVAGVDRGGKGFVPVGSSTFQEGDYAAVIVQKDALDTLDQLLQPIGE
                     H"
     gene            complement(5313..7679)
                     /locus_tag="VF195_11500"
     CDS             complement(5313..7679)
                     /locus_tag="VF195_11500"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6581482.1"
                     /translation="MARRSPGPRGPVHLWKRLVLGPALPTAQLQHERLGKPTALAVFA
                     SDNLSSVAYATEEILKVAVPAIGILAFSVVMPITFGILAVLAILLFSYRQTIRAYPSA
                     GGAYIVTKDNFGLLAAQLAGVALLTDYVLTVSVSVAAGVAAITSAAPELYTYRVWLSV
                     VCIWFIAWGNLRGVRESGRMFAVPTYFFIVMMFLLLGLGLAKSLAGDLHPMPIPADVA
                     ETTGAISLFLLLHAFASGGAAVTGVEAISNGVPAFKPPAWRNARTTLMWMGSLLGVMF
                     LGLSYLAVKLEVVPTATKTVISDVGRAVFGSGGLGTALFLMLQVATTLILILAANTSF
                     ADFPRLANFHAKDDFMPRPLTKRGHRLVFSNGIIGLAVASTALVIVFQADVHKLIPLY
                     AIGVFLSFTLSQAGMAKRHRRLREPGWKHGLFINGLGAVTTAVVTVVIAITKFADGAW
                     AVMVFVPVMVWILVRMNHMYAREHAELDLNLHTFEREPLARPVSVVIVENLDRKTIHA
                     LKYAKTIRSSSTYALHVEDEPAKSEHLRRAWTELGLDVPLRILPRRSDAARAIAAYVG
                     TLAGDADVTVIVPAPARLGRFERLRRGRTGARLARMLAANPRVRVTLVRDHPHAGDEG
                     GQRALLPRAAHRAVVLVDRPDRAALQAVRYALSLGAEEVVAVHAAVDPDVQDELISRW
                     MELRMPIALDLVECWDRDVARSVERYVVDLMGPRTEVTVVLPRRDFARVTQRLLHDRT
                     SRSIARTLARYEHVDVAVVPYFFGSERPLPPPEGSPSDRAPAAADQAG"
     gene            7826..9301
                     /locus_tag="VF195_11505"
     CDS             7826..9301
                     /locus_tag="VF195_11505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581483.1"
                     /translation="MGDRWVLAPGGGSRMRALVISVASPSLALGIALIVQPERELGAV
                     SLFLLAVVAASVAGGIWAGIGSSALGFLALNYFFTEPLHTLRVRNRDDVVALVVFLIV
                     ALLVGWVVARAVSERDRATRREREARLLNLFATKALSGEPLDRVLNDLAAALVDALRL
                     ASCAIHASAAGRIYEVRRSRPGTTGGLAVEVALSSGEGEFGSLAASPPVGEDLVPEDR
                     RLLEAAASQIAVYLERARLDAEIAAARLDAERSQARAALFSSVTHDLRTPLASIKTAV
                     TSLLQVEVRFDPAQARELLQTVLEETDRLNRLVGNILGLAQVRSGALLPVKEPTALDE
                     VVESVLHRLEPTLGGVRVRTIFRDAPDVPVDPVLVDQALSNLIENAIRFSPPSGEVTV
                     SVTPWRSGVQVRVADQGPGIAPEDRDRVFEPFTQLGTSAPDATGGSGLGLAISRAIVL
                     AHGGRIWIEGTPGGGTAVVFELPARGAAPVSQEAATREARR"
     gene            9298..9999
                     /locus_tag="VF195_11510"
     CDS             9298..9999
                     /locus_tag="VF195_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009538845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581484.1"
                     /translation="MTRVLVVDDEPQIRRAMRTSLEAHGYEVRTVGTGEEAVVAAAED
                     DPELVLLDLGLPDLDGTEVIRRVRSFSEVPIIVLSVRDRQVDKVAALDAGADDYVTKP
                     FGVEEVLARLRAALRRSQAGEPSPPLLRFGEVEVDLGRRLVSLAAEPVHLTKTEYALL
                     EAFVTNPGKLLTHQWLLRKVWGPGYAEESHYLRVYVRSLRRKLGDEASAPRLILTEPG
                     VGYRWIAVRTEGDPS"
     gene            10011..12341
                     /locus_tag="VF195_11515"
     CDS             10011..12341
                     /locus_tag="VF195_11515"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6581485.1"
                     /translation="MSTTTTPSVLKRLLVGRAFTSHQLEHTLLPKTLALPVFASDALS
                     SVAYATGEIFIQLTIVSLAFKHLVMPISIAIAALMAIVVISYRQTVRAYPSGGGAYIV
                     SKDNLGAPYGLVAAAALLVDYMMTVIVSIVAGVFAIGSAFPAANDHRVVLSIAFVWLV
                     TLANLRGTRESGTLFAVPTYGFVVSILIMIVLGFIQCLGGCPTTEAVEPIHNAATTAS
                     AIGLFAILRAFSSGATALTGVEAISNGVPAFRRPQARNAAETLAIMGVIAITMFVGIS
                     WLSVHVHGTVASDVRSIPGQIAWAVFDGGLGFYVVQFFTAAILILAANTAYQDFPRLS
                     SILARDRYMPSQFMNRGDRLVFSNGIVVLAIGSSILIYAFNADLTRLIQLYVIGVFTS
                     FTLSQSGMIRHWLRERHKGASAARGWRRSIVINCVGAVATAVVLIVVTVTKFTAGGWL
                     SMLFMAILIVMFVSIHRHYESVGRQLRRGAVRPGEAGVNHIVLVIRELDAAAAEALGA
                     VRSIRPTELHIVYPTSDGIPPDLEQRWPQFSMGAAPLEPISLGKDSPLDALRRYVRGI
                     ERAPADFVNVVIPETVPGSLLLYLLRRAAVVRLKAGLLRERNIVVTDVPVVVRDGEPV
                     GVDALPLIPQRTVALVFVSAVHDATVRAVNYAQTLEASETRAIYFDLDPEASHRMQEE
                     WGESGMQIPLHIVEAPFRDLTRPMLDEVRRFTSRPGTIVLVVVPELIVSKWRHLLLHN
                     QNALFVKRLFLFEPSVVLSDVPYVLSDREREEVPAG"
     gene            12341..>12780
                     /locus_tag="VF195_11520"
     CDS             12341..>12780
                     /locus_tag="VF195_11520"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX6581486.1"
                     /translation="MDREAAVALCREKCLKDTTVRHLISVEGVMRGLARRFEQDEELW
                     GLTGLFHDLDQDHTGDAAANHARLAAEWLREAGVDERVINGVLAHAYEEYRTDLMSKA
                     VVHADAVAGLLVAAALVRPERSSGMKVSSLKKKLKEKAFAPGVNR"
BASE COUNT         1999 a         4301 c         4410 g         2070 t
ORIGIN      
        1 gtatagccgg cgaaggccga gccgttgttg ttcccctgag agatgaacgc gtagaagatc
       61 tcgctgaacc cgtgcggtcc ggcgttcagt cgcccgtcca gtcctccggg gatcaccacg
      121 cccaccccga tgaccgcaag ggcgaggacg aacggcacga gcacggcgat cgccgagatc
      181 ttgacctcac gagcgccgat cttcttgccc aggtactcgg gcgtccgccc gaccatcagg
      241 ccgccgatga acacggcgat gatcgcgtag aagatcatcc cgtacaggcc gctgccgacc
      301 ccgccccaga tcacctcgcc gatggccatg ttgaacatcg gcgcgagagc cccgagcggc
      361 cggtacgagt cgtgcatcga gttgacgctg ccgttcgaag cggcggtcgt cgcgaccgcc
      421 cacaaggagg actcgtcgac accgatgcgg agctccttgc cctccaggtt ctgcgccgag
      481 gtcgggaccc cggccgccgc catcgcctgc gaggagccgt gctcctgtgg gacggtcagc
      541 gcgagcccgc cgaagaacag gaccatcatg accgcgaaga tcgcccatcc ctggcgcacg
      601 ttgcccaccc acttgccgaa cgtgtaggtg agcgccgccg ggatggccag gatcgcgaac
      661 agctcgatga agttgcccaa cggacccccg ccctcgaacg ggtgcgccga gttcacgttg
      721 aagaagccgc cgccgttggt tccgagctgc ttgatcgcga tctgcccggc ggccggtccg
      781 cgcgcgatcg tctgctcgaa cccctggata ccggtcgccg cctcgttggc accaagcgtc
      841 tgcaccactc cctgggtgac caggatcacg gcgacgagcg ccgagatcgg caggaggatg
      901 tacaccgtgc cgcggacgag gtcccgccag aagttgccga cctcctcgcg cttggacctg
      961 gtgaaaccac ggaacagcgc gacgagcacc gccatgcccg cggcggccga cacgaagttc
     1021 tggctcgtca acgcgagcat ttggctgagg tagctcatcg tggtctcgcc gccgtacgcc
     1081 tgccagttgg tgttggtcgt gaagctggag gcggtgttga aggacacgta ctggtcgacg
     1141 gctccgagcc ccacggggtt caggggcaga tctccctgca cccggaacac gacgtagagc
     1201 acgaccatcg agatcgcgga gaaccacagg cacgaggtgg cgtagcgtcg ccaggactgc
     1261 tcgagcgtcg ggttcacacc gatcagccgg tacacgatcc cctcgaggcg accgatctcc
     1321 tcgcgacggt agacgcgata catgaaggca cccagcgaag gggtgaccgc gatcagcagc
     1381 acgatgtaga cgacgatcgt gaccacgtca gaacttctcc ggacggatca gtgcgtacgt
     1441 gacgtacacg aggagggcga cagcgaggat gagctcgaga acgtcggcga ggctcatacg
     1501 cgttccagcc aggcgacgaa cgcgaagagt gcagcgaacg acacgatcgt cagggtgagc
     1561 atcacgacgt cgagcattcc gaccccctgc ctcgcatgat ccgtccagct taggtttggg
     1621 gtcgtcaggg acccgtaacg aacgagccac tccgcgtatg ggatgtgtga agagggcatc
     1681 ccggtctctc ggcgtcacgg tgtgggtgag atgctcctct ttgaggagtg ttgagcggaa
     1741 tgagcgagca gatcgaccgg tcctcgacca gccaggtgtt gcggctgatg cggccccctc
     1801 gaccgcgccg gcaggcggtc gcctacctgg tcgcggtcct cggtacattc gctctcgcgg
     1861 cgagcctggt cccgttccgg gacagcatcg atccgctggc gaaggggttc ggattcctgg
     1921 tggtcgtcgt cgccgccgcc gggatcggag ggatcgggcc cggcgtggtg gcgtcgatgc
     1981 tcgggttcat cgtcttcaac ttcctcttcc ttcccccgta caacaccttc gtgatcggca
     2041 agcccgagta cgtcgtggtc ctgttcgtga acctcggcat ctcggtcctc atctcgatcc
     2101 ttctcgggcg agcgaccgag cgggccgccg ccgccgaggc gcgggaggca gagctccgca
     2161 cacttcagga cctttcgcgc gaactcgtcg tgcgcgcacc gggaacggag gcctaccgcg
     2221 agatcatccg ggacatcctc gacacgttcg ggtacgaggc cggtgccttg ttcgtccgcg
     2281 actcggcccg gtcggggggt ttgcgcgagg aggtctccgt cggctgcagc cccggcgaca
     2341 tcgtccccga atggaatccg cgttctactg agcgtcctcc cgaacgtctc ccgctgtcga
     2401 tcggtccgaa caacgtcggt cttctggtgc tgcgaggagc tcgccccgcc ccgtcggagg
     2461 ccgagagccg gatcctccgg gcgctcggcg accagctcgc gctcgtgctg gaacgcgatc
     2521 ggctcctgcg aaccgcaacc gacgcagagg tctatcggca gaccgagcga ctgcggcaaa
     2581 cgctgctcgc cgccgtctcg cacgatctcc ggagcccgct ggcagcgatc aaggcgagcg
     2641 tgtcggatct gctcgaggag ggggtcaccc gttcacccga ccagcagcgg gaggcgctcg
     2701 aagcgatcga ctcggagagc gaccggctga acgcgctcat cgccaacctc ctggacatgt
     2761 cgcggatcga ggccggtgtg ctccgcgcca gggtcgaggc ggtcgacctc ggcgacgccg
     2821 tcgcgacgag tgcccgcaac gtcagccggg tgtggccgga cctcacggtt cgcgaccggg
     2881 tcgaggacgg ggtcatggtt cgtgccgatc ccgtgttcct ggaccgagtg ctgacgaacc
     2941 tgctcgacaa cgccgcgcgg tcggccaagg cggcggcagc ctcggacgtc gaggtgacat
     3001 ccaagcagac cgacggccgc gtcacgatcc gcgtgatcga tcacgggaac ggcatccccg
     3061 ccgaggcgcg cgaggtcctg ttccatccgt tctacgagct cgatcagcgc aacccgcgcc
     3121 tgggtgccgg attgggactc gcgatcgcga aggggttcgc cgtcgcgatg gacggtgaga
     3181 tctggatcga ggagaccccg ggtggcggtg cgacgttcgc ggtggccgtg cccgtcagcc
     3241 cggcgagtcc gtgagcacga tcctcgtcgt cgacgacgaa gagcagatcc tccgggcgct
     3301 aaggcgagcg ctcaacgccc gcaggtacga ggtcgtcacg gctgccgacg gcgaggatgc
     3361 gctcgtcgag gccgaggcct cgatgcccga cctcgtggta ttggacctca acctgcccgg
     3421 gatcgacggc atcgaggtat gccggcgcct ccggtcgtgg acccaagtcc cgatcctcgt
     3481 gctgtccgtg cgggaggacg aacgcgcgaa ggtgcaggcg ctggatctgg gcgcggacga
     3541 ctacctgacg aagccgttcg gcatcgagga gctgctcgcg cgggttcgcg cgttgctccg
     3601 tcggagtcag tcggaggagg ggcaggcttc gaggttcgag gccggcggag tgctcatcga
     3661 cctcgccgcc cgccgggtcg ttcgtgacgg caccgacgtc catctgacca ggacggaatg
     3721 gacgctgctc ttggcgatgg ccgagcaccc cggcaagctc ctcacccacc ggtggctcct
     3781 ggaacgagtg tggagcaccc ggtacgggga ggacgtcgag gtcctacgtg tcttcatcag
     3841 tcagctccgc aagaaggtcg agccgaaccc gagcgaaccg agcatgatca tcacggatcc
     3901 cggggtgggg taccgctggg ccgctcaagc gaagggcgaa gacaactccc ggtccgaggg
     3961 atgagctagg ccggttcctc gccgaggacg gccgcgcgaa gggcgctctc ggcttccggc
     4021 accgtgagcg cgagcacctc gtcgccgtcg gtgatcacgg tctccggctg ggggatcacc
     4081 acgtggctct cccggatgat cgccacgatc gtggcgtcgg gcgggaggcg catctcgtac
     4141 aaggggcggc ccgccgacgg cgagtcggcg gggatggtca gctcgacgat cgagatcccg
     4201 cctcgctcga gcgtgaggag gcggacgaga tcgccgaccg tcacctcctc ctccaccatc
     4261 gcggtgagga tgtgtggagg cgagaccgcc gcgtccacgc cccactgctc ggtgaacagc
     4321 cactcgttgt cgggatggtt cacccgcgcg agcaccttgg ggacgccgaa ctcctgcttc
     4381 gcgagcagcg ccgtaactag gttgtcctcg tcgtcgccgg tcgacgcgac gacgacatcg
     4441 gcggtgttga gttgagcctt ctcgagcacc cacggttcgc aggcgtcgcc gatcatgtga
     4501 tcgacctgcg gcgcccactc cttggcgatc tcgaggacgg cggggtcctg ctcgatcagc
     4561 gtgaccgagt ggccccgctt ggcgaggtcc gacgcgaggt gccggccgac cgctcctgcc
     4621 ccgctgatca cgacccgcat ctcagtgctc cccgatgggc tgcaggagct ggtcgagggt
     4681 gtcgagcgcg tccttctgca cgatcaccgc ggcgtagtcg ccctcctgga acgtgctcga
     4741 ccccacgggg acgaacccct tgcccccccg gtcgacgccg gcgacgagga tcttgccctc
     4801 caggttgagc gatgagaccg gcttcccgac gagatgcgcg ggaatctcga ctcgcatccg
     4861 gaacaggtca ccggccgcca gggtctcctt gatctcctca ccggggtgcg tgagcatcag
     4921 gatcatctgc ttcacgcccc agcgggtggt ggagaccgtg gggatgttca ggcgctcgta
     4981 gatgtccgcg cgcatggggt cgtagatccg agcgatgacc ttcgggacgt ggtagtgctc
     5041 gcgggcgacg cgcgccgaga cgatgttgga gttgtcaccg ctcgagaccg cgaggaaggc
     5101 gtccgcctcc ttgatgcccg cttcctcgag gacgtcgcgg tcgaatccca ggccgacgat
     5161 ggtgcgcgcc tcgaaccccg gaggcagccg tgcgaacgcc gaggcgttct tgtcgatcac
     5221 ggtgatctcg tgcccggcct tcgagagctc ctgggtgagc aggatgccga cacgaccaca
     5281 gcccatgatg accacacgca cggctgggta ccttagcctg cctggtcggc ggccgcaggc
     5341 gcccgatccg acggcgaccc ctccggggga ggaaggggcc gctccgatcc gaagaagtag
     5401 ggcacgaccg cgacgtccac gtgctcgtac cgggcgaggg tccgcgcgat gctccggctc
     5461 gtccgatcgt ggaggagacg ctgggtgacc cgtgcgaagt cgcgccgagg aagcacgacg
     5521 gtgacttcgg tgcgcggtcc catgaggtcg acgacgtacc gttcgaccga cctcgcgacg
     5581 tcgcggtccc agcactccac caggtcgagg gcgatcggca tcctgagctc catccatcgc
     5641 gagatcagct cgtcctggac gtccggatcg accgccgcgt gcacggccac gacctcttcg
     5701 gcgccgagcg acagggcata gcgcaccgcc tgcaacgccg cccggtcggg tcggtcgacc
     5761 aggacgacgg ctcggtgtgc ggcacgcggc agcaacgccc tctggccgcc ctcgtcgcct
     5821 gcgtgaggat gatctcggac gagcgtgacg cgaacgcgcg ggttcgcagc aagcatccgg
     5881 gccaaacggg ctcccgtacg tccgcggcgc agacgttcga agcgtcccaa tcgggccgga
     5941 gcggggacga tgaccgtcac atcggcatct ccggcaagcg tgcccacgta ggcggcgatc
     6001 gccctcgcgg cgtcgctccg tcgcggaagg atccgaagcg gcacgtcgag gccgagctcc
     6061 gtccacgcac ggcggaggtg ctcgctcttc gcaggttcgt cctccacgtg gagggcgtag
     6121 gtggagctcg agcggatcgt cttggcgtac ttgagcgcgt ggatggtctt ccgatcgagg
     6181 ttctcgacga tcaccaccga gaccggacgg gcgagcggct ctcgctcgaa cgtgtggagg
     6241 ttgaggtcga gctcggcgtg ctcgcgtgcg tacatgtggt tcatccgaac gaggatccac
     6301 accatgacgg gaacgaacac catcaccgcc cacgcaccat cggcgaactt cgtgatcgcg
     6361 atcaccaccg tgaccacggc ggtcgtgacg gctccgaggc cgttgatgaa aaggccgtgc
     6421 ttccatcccg gctcgcgaag gcgacggtgg cgcttcgcca tgccggcctg ggagagcgtg
     6481 aacgacagga acaccccgat cgcgtacagg gggatcagct tgtggacgtc cgcctggaac
     6541 acgatcacga gcgccgtcga ggcgaccgcg agcccgatga tgccgttgga gaacacgagc
     6601 cggtgcccgc gtttggtgag cggacgcggc atgaagtcgt ctttcgcgtg gaagttcgcg
     6661 agcctcggga agtcggcgaa cgaggtgttc gccgcgagga tcaggatgag ggtcgtggcc
     6721 acctggagca tcaggaacag ggctgtcccg agccccccgc tgccgaacac cgccctcccg
     6781 acgtcggaga tgacggtctt ggtcgccgtg ggcaccacct cgagcttcac cgcgaggtag
     6841 gacagtccga ggaacatcac accaagcagg ctccccatcc acatcaacgt cgttcgggcg
     6901 ttccgccacg caggaggctt gaaggccggg acgccgttgg agatcgcctc gaccccggtg
     6961 acggcggcgc ctccggaagc gaaggcgtgc aggagcaaga acaggctgat cgcgcccgtc
     7021 gtttcggcca cgtccgccgg gatcggcatc ggatgcaggt cgccggcgag cgacttcgcc
     7081 aggccaaggc cgaggagcag gaacatcatc acgatgaaga agtaggtggg gacggcgaac
     7141 atccgccccg actcgcgcac gccccgcagg ttgccccagg cgatgaacca gatgcatacg
     7201 accgagagcc acacccggta ggtgtagagc tccggcgccg cagatgtgat cgcagccacc
     7261 cccgcggcca cggagaccga aacggtgagc acgtagtccg tgaggagcgc cacgccggca
     7321 agctgggccg cgaggagtcc gaagttgtcc ttcgtgacga tgtaggcccc gccggcgctc
     7381 gggtaggccc tgatcgtctg gcggtacgag aagagcagga tcgcgagcac ggccaggatg
     7441 ccgaaggtga tcggcatcac gacggagaaa gcgaggatcc cgatcgccgg cacggcaacc
     7501 ttcaggatct cctcggtcgc gtacgccacc gaggacaggt tgtcgctcgc gaacaccgcc
     7561 agggcggtcg gcttgccgag tcgctcgtgc tggagctgcg ccgttggcag ggccggcccg
     7621 agcacgagtc gtttccacag gtgcacgggc ccccgcggtc cggggctgcg tctcgccatg
     7681 ggcggccgct tcgtttcggg ttgtcggagc ttggtggcca cggagcctcc tcgtcgcccc
     7741 aagcgtcggt cgtgccgcgt aaacgaggcg tgaaaggccg gggcttccgc gtgaagaagc
     7801 cgtagcgaag ccgtagcgtg gccgcgtggg cgatcggtgg gtgctggctc ccggtggagg
     7861 ttcgcggatg cgggccctcg tgatctcggt ggcgtcgccc tcgctcgcac tcgggatcgc
     7921 gctgatcgtc cagcccgagc gcgagctcgg ggcggtctcc ctgttcctgc tggccgtcgt
     7981 ggcggcgtcg gtcgccggag ggatctgggc gggcatcggg agctcggcgc tcggcttcct
     8041 ggccctgaac tacttcttca ccgaacccct ccacacgctc cgggtccgaa accgggacga
     8101 cgtggttgcg ctcgtggtgt tcctcatagt ggccttgctc gtgggctggg tggtggcccg
     8161 cgccgtctcc gaacgtgatc gtgcgacccg tcgtgagcgt gaagctcgcc tcctgaacct
     8221 gttcgcgacc aaggcgctct ccggcgagcc gctggaccgc gtgctgaacg atctcgcggc
     8281 ggcgctcgtc gatgcgctcc gtctggcgag ctgcgcgatc cacgcctctg cggccggtcg
     8341 catctatgag gtccgtcgtt ctcgccccgg aacgaccgga gggctcgccg tcgaggtggc
     8401 gctctcgagc ggcgagggcg agttcgggag cctcgcggcg tcacccccgg tcggcgagga
     8461 cctcgttccc gaggatcgcc ggctgctcga ggcggccgcg agccagatcg ccgtgtacct
     8521 ggagcgcgcc cgcctcgatg cggagatcgc cgccgctcgg ctcgacgccg aacggagtca
     8581 ggcgagggcc gcgttgttct cgtcggtcac ccacgacctc cgcacgccac tggcgtcgat
     8641 caagaccgcg gtgaccagtc tgctgcaggt cgaggtgcgg ttcgacccgg ctcaggccag
     8701 ggagctcctg cagacggtcc tcgaggagac cgaccggctc aaccggctgg tcggcaacat
     8761 cctcgggctc gcgcaggttc gctcgggcgc gctccttccg gtgaaggagc cgacggcgct
     8821 cgacgaggtg gtcgagtcgg tcctgcatcg cctcgagccg accctcggcg gggtccgggt
     8881 ccggacgatc ttccgcgacg ccccggacgt ccccgtggac ccggtcctgg tcgaccaggc
     8941 cctgtcgaac ctcatcgaga acgcgatccg tttctcgcca ccgtccggcg aggtcacggt
     9001 ctcggtcacg ccgtggcggt ccggcgtgca ggtgcgggtg gcggatcagg ggcccgggat
     9061 cgcgccggag gaccgcgacc gcgtgttcga gccgttcacg caactcggga cgagcgcccc
     9121 ggacgcgacg ggcggcagcg gtctgggcct ggcgatctcc cgagccatcg tgctcgcgca
     9181 cggtggcagg atctggatcg aggggacccc cggcggtggc accgcggtgg tgttcgagct
     9241 ccccgcccga ggcgcggctc ccgtctcgca ggaagcagcg acccgggagg cacgtcgttg
     9301 acgcgcgtgc tcgtcgtcga cgacgagccg cagatccgac gcgcgatgcg gaccagcctg
     9361 gaggcgcacg gctacgaggt gcgcaccgtc ggcacggggg aggaggccgt ggtcgcggcg
     9421 gccgaggacg atcccgagct cgtcctgctc gatctcggtc tcccggacct cgacggcacc
     9481 gaggtgatcc ggcgcgtgcg gtccttctcc gaggttccga tcatcgtgct ctccgtccgg
     9541 gaccgacagg tcgacaaggt cgcagcgctc gatgccgggg cggacgacta cgtcaccaag
     9601 cccttcgggg tcgaggaggt cctcgcccgc ctgcgcgccg cgctccgccg tagccaggcg
     9661 ggcgaaccgt ctccccccct cctgcggttc ggcgaggtcg aggtcgacct gggtcgccgc
     9721 ctcgtttcgc tcgccgcgga gcccgtgcat ctgacgaaga ccgagtacgc gctgctggag
     9781 gcgttcgtca cgaacccggg gaagctgctg acccatcagt ggctcctccg caaggtgtgg
     9841 gggcccgggt acgcggagga aagccattac ctccgcgtgt acgttcgctc gctccgccgc
     9901 aagctcgggg acgaggcgtc ggcaccccgg ctcatcttga ccgagcccgg cgtggggtac
     9961 cggtggatcg ccgtacggac cgagggcgac ccgtcgtagc atcaccatcg atgtcgacca
    10021 cgaccacgcc gtcggtcctc aagcgcctcc tcgtggggcg ggcgttcacg agccatcagc
    10081 tcgagcacac gctcctgccg aagacgctcg ccctgccggt gttcgcctcg gacgcgctct
    10141 cgtcggtggc gtacgcgacg ggcgagatct tcatccagct cacgatcgtg agcctggcgt
    10201 tcaagcacct cgtgatgccg atctcgatcg cgatcgcggc cctgatggcg atcgtcgtga
    10261 tctcgtaccg ccagacggtg cgggcgtacc cctctggcgg cggcgcctac atcgtcagca
    10321 aggacaacct tggcgcgcca tatggcctcg tagcggccgc cgcgctcctc gtggactaca
    10381 tgatgacggt gatcgtgtcg atcgtggcgg gggtgttcgc gatcgggtcg gcgttcccgg
    10441 ccgccaacga ccaccgggtg gtcctgtcca tcgcgttcgt gtggctcgtc acgctcgcca
    10501 acctccgagg aacgagggag tccggaacgc tgttcgccgt ccccacatac gggttcgtgg
    10561 tgtcgatcct gatcatgatc gtgctcggat tcatccagtg tctaggaggt tgcccgacca
    10621 cggaggcggt cgagccgatt cacaacgcgg ccaccaccgc gagcgcaatc ggtctgttcg
    10681 cgatcctccg cgcgttctcc tcgggcgcta ccgctctgac gggcgtcgag gcgatctcga
    10741 acggcgttcc cgcgttccga cgaccgcagg cgcgcaacgc ggcagagacg ctggcgatca
    10801 tgggcgtgat cgcgatcacc atgttcgtcg gcatctcgtg gctgtcggtc cacgtgcacg
    10861 gaacggtcgc gagcgacgtt cggtcgatcc ccgggcagat cgcgtgggcc gtgttcgacg
    10921 gagggttagg cttctacgta gtccagttct tcacggcggc gatcctgatc ctggccgcga
    10981 acaccgcgta ccaggacttc ccgaggctct cgtccatcct ggcgcgtgac cgctacatgc
    11041 cgagccagtt catgaaccgg ggcgaccgcc tcgtgttctc caacggcatc gtcgtcctcg
    11101 cgatcggctc gtcgatcctg atctacgcgt tcaacgccga cctgacgcgg ctgatccagc
    11161 tctacgtgat cggcgtgttc acctcgttca cgctgtcgca gagcgggatg atccggcact
    11221 ggctccgcga gcgacacaag ggggcgtcgg ccgcccgcgg gtggcggcga tcgatcgtga
    11281 tcaactgtgt cggcgccgtc gctacggcgg tcgtcttgat cgtggtcacg gtcacgaagt
    11341 tcacggcagg tggctggctc tcgatgctgt tcatggcgat cctcatcgtc atgttcgtct
    11401 cgatccatcg tcactacgag tccgtcggca ggcagctacg tcggggggcg gtgaggcccg
    11461 gcgaggccgg cgtgaaccac atcgtcctgg tgatccgaga gttggacgcc gcggcggccg
    11521 aggccctggg ggcggttcgc tcgatccgcc cgaccgagct tcacatcgtc tatccgacga
    11581 gcgacgggat ccctcccgac ctcgagcagc ggtggcctca gttctcgatg ggcgcggcgc
    11641 cgctcgagcc gatctcgctc ggcaaggact cgccgctcga tgccctccgc cggtacgttc
    11701 gcgggatcga gcgggcgccg gcggacttcg tgaacgtcgt catccccgag acggttcctg
    11761 gctcgctcct cctctacctg ctgcgccggg cggcggtggt tcgcctgaag gccggcctgc
    11821 tgcgtgagcg caacatcgtc gtcaccgacg tccccgtcgt cgtgcgggac ggggagccgg
    11881 tgggcgtgga cgccctgccc ctgatccctc agcgaacggt ggccctcgtc ttcgtctcgg
    11941 ccgtccacga tgcgaccgtt cgcgccgtga actacgcgca gacgctcgag gcctccgaga
    12001 cgcgagcgat ctacttcgac ctggatcccg aggcctcaca ccggatgcag gaggagtggg
    12061 gtgagagcgg gatgcagatc ccgctgcaca tcgtcgaggc gcccttccgc gacctcacac
    12121 gtccgatgct cgacgaggtc cgacgattca cctctcgtcc cggaacgatc gtgctggtgg
    12181 tcgttcccga gctgatcgtg tcgaagtggc gacacctctt gctccacaac cagaacgcgc
    12241 tgttcgtcaa acggctgttc ttgttcgagc ccagcgtcgt cctctcggac gtgccgtacg
    12301 tcctctcgga ccgggaacgg gaggaggtcc cggccgggtg atggatcgcg aggccgccgt
    12361 cgcgctctgc cgcgagaagt gcctgaagga caccacggtc cgacacctga tctcggtcga
    12421 gggtgtgatg cgcgggctcg cccggcggtt cgaacaggac gaggagctct ggggcctgac
    12481 gggactgttc cacgacctgg accaggacca cacgggcgac gccgccgcca accacgcccg
    12541 cctggccgcc gagtggctcc gcgaggccgg cgtggacgag cgcgtgatca acggcgtgct
    12601 cgcgcacgcc tacgaggagt accgcaccga cctgatgagc aaggccgtcg tgcacgccga
    12661 cgccgtggcc gggctgctcg tcgcggcggc gctcgtccgg cccgaacgga gctcgggcat
    12721 gaaggtctcg tcgctcaaga agaagctgaa ggagaaggcg ttcgcgcccg gggtcaaccg
//
LOCUS       DASUYD010000217         6009 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1034463, whole genome shotgun sequence.
ACCESSION   DASUYD010000217 DASUYD010000000
VERSION     DASUYD010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6009
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034463"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VF195_11525"
     CDS             complement(<1..195)
                     /locus_tag="VF195_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014156177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX6581487.1"
                     /translation="MAACPKCGSENAEGANFCSVCGERLGAAEAPAEVRKIVTIVFCD
                     VTGSTELGEQLDPESMRKVMA"
     gene            complement(286..2046)
                     /locus_tag="VF195_11530"
     CDS             complement(286..2046)
                     /locus_tag="VF195_11530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581488.1"
                     /translation="MQRRRILVSTFLAVALTATATTSLAASNGGFKTGRPSMLTAVKA
                     GVQITPLLTVGDVFGESGFRFEAIPDGISLRTRGQGRVDVYVNHETSKVPFPFNTTTP
                     TAANGESDFDNSQVSHLILNRNSAGVLDGSFVIPSSGGFQRFCSNFLATSKEGFDREI
                     LFTNEESPDYVFRQEDSWPPPIGDPDEEESGVVLALDVKSRQYHVIYGMGRHNHENSV
                     AIPGFDDLVVLSGDDTFTSGPLTIPPGGPNTETSAASQSQLYSYIAPDTDSLLADEGD
                     LWAFVSDDPSFDDYYDFVPGSAQTVSGHFIEVPKNIATGKNPDGSEITSVDVGYPAPP
                     TDGSWQRDLRTTVPTGIDGPQWVLEYWSQLNNVFDFVRVEDTAYDKRPGMGNVVYIVD
                     SGRGTAGISQSGRSTNGRVWKMVLDPDDPTVVDSLSIAVEGDDNPVKTLGEIHQPDNI
                     DTTQTGLLVTEDPGSSQQFVLADQGLPNATTARLWYVPFSGASPEVVVKIDQSADGEG
                     TDVDGRPDGNWGAWETSGIVDASAAFGAGAFLIDVQAHTLWVEKADGPDTFIDAETVN
                     PDFTFKREGGQLLLIRIPGI"
     gene            2262..2681
                     /locus_tag="VF195_11535"
     CDS             2262..2681
                     /locus_tag="VF195_11535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HEX6581489.1"
                     /translation="MRTNLTLEDLPGFLDLPLVAVLATLRQDGSVQLSPVWHEWRDAG
                     FNLWLPAADVKTRHLRRDPRASLVVAESETPLRGVELRTEARLIDRDVRATAVRIATR
                     YVGGAKGIEYVGSAENDVLARLEPGHLRVWDFADEYD"
     gene            2858..4243
                     /locus_tag="VF195_11540"
     CDS             2858..4243
                     /locus_tag="VF195_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019872149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6581490.1"
                     /translation="MVSPSSISISKLRADVDGRVIAPEDAEYDQARALFYGGMDRHPA
                     AIVRVKDDMDIVRVVALARESGLELAVRSGGHSIPGHSVSEGGIVIDLADMRALDIDV
                     EARTTWAQTGLTALEFTTAAAEHGLGVGFGDTGSVGIGGLTLGGGVGYLVRKYGLTID
                     DLLAADIVTADGEIHHVDAEHEPDLFWAIRGGGGNFGVATRFRFRLHEIDTVVGGMLL
                     LPATVDTIAGFMAEAEAAPEELSSIANIMKAPPMPFVPEEAHGKLVIMALMTYAGDPE
                     AGQGALAPFRALAEPVADLLRPMPYPEVYPPEEEEYHPVATARTMFVDTIGRAEAETI
                     LEHLATSTAMMGVAQLRMLGGAMARVPANATAFAHRQSKIMVNVAALYERPDEVAVHG
                     PWVEAFAAALRQGDDGAYVNFLADEGEARIRAAYPGTTWDRLRQIKVRYDPTNLFRLN
                     QNIPPAVEGFS"
     gene            4240..5031
                     /locus_tag="VF195_11545"
     CDS             4240..5031
                     /locus_tag="VF195_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017949472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6581491.1"
                     /translation="MNTVTSKDGTAIAFDRSGDGPPIVMVGGALSTRRAAETLAGLLA
                     PSFTVYAYDRRGRGDSGDMPPYAVEREVEDIYSLVEDAGGSAVAFGHSSGGVLALEAA
                     AHILGITKVALYEPPFIVDASRAPLPDDYIERLTELASTDRPGDAVEYFMITGPGVPA
                     EAVASMKDAPFWSALEAVAHTLSYDGRIMGDQMAGTSLPSDRWASVTIPTLVIDGGAS
                     PLWLGNAAQGLVDALPDARRRTIEGQTHDVDPVLLAPVLEEFFAG"
     gene            5053..5511
                     /locus_tag="VF195_11550"
     CDS             5053..5511
                     /locus_tag="VF195_11550"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6581492.1"
                     /translation="MDDLAVVARGIIDGNRYMVLGTSDAEGRPWVSPVYFTPSGYTEL
                     YWVSSPEANHSRNLARRPELSIVVFDSRVAVGEGQGVYMSAIAQELTGADLERGIEIF
                     SRVSESHGAKPWTVDDVRPPKPYRLYRARVSEHWVLDPESRPDQRTPVQP"
     gene            5768..5962
                     /locus_tag="VF195_11555"
     CDS             5768..5962
                     /locus_tag="VF195_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013230123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581493.1"
                     /translation="MLVGDELVVKLPKERVDELVDAGVAKRFDAGKGRPMKEWASIAP
                     SASRRWRGLVDEARAFVGGR"
BASE COUNT          976 a         1977 c         2100 g          956 t
ORIGIN      
        1 agccatcacc ttgcgcatcg actcggggtc gagctgttcg ccgagctcgg tcgagccggt
       61 cacgtcgcag aacacgatcg tgacgatctt gcggacctcg gccggtgcct cggcagcgcc
      121 caggcgctcg ccgcagaccg agcagaagtt cgccccctcg gcgttctcgg agccgcactt
      181 cggacaggcc gccacggcgg gagtctaggc cgtgctcacg aacatggtcg ggtcggcagg
      241 ccgtgaacga aaggggcgcc ccgaagggcg ccccctcacg taccgctaga tcccggggat
      301 gcggatcagc agcagctgcc cgccttcgcg cttgaacgtg aagtcggggt tgacggtctc
      361 ggcgtcgata aaggtgtcgg gcccatcggc cttctccacc cacagcgtat gcgcctgcac
      421 gtcgatcagg aacgcgccgg caccgaacgc cgcggaggca tccacgatcc ccgacgtctc
      481 ccacgcgccc cagttgccgt cggggcgccc gtcgacgtcg gtgccctcgc cgtcggcgga
      541 ctggtcgatc ttgacgacga cctcgggaga agcacccgag aaggggacgt accacagacg
      601 cgccgtggtc gcgttgggaa gcccctggtc ggccagcacg aactgctggc tcgatccggg
      661 gtcctcggtc actaggaggc cggtctgcgt cgtgtcgatg ttgtccggct ggtggatctc
      721 gcccaaggtc ttgaccgggt tgtcgtcacc ctcgaccgcg atgctgagcg agtcgaccac
      781 cgtcgggtcg tccggatcga ggaccatctt ccagacgcga ccgttcgtcg agcggccgga
      841 ctgcgagatg ccggctgttc cacggcccga gtccacgatg tacacgacgt tccccatacc
      901 aggacgcttg tcgtacgcgg tgtcctcgac gcggacgaag tcgaagacgt tgttgagctg
      961 gctccagtac tcgagtaccc actggggacc gtcgatgccg gtcggaaccg tcgtgcggag
     1021 gtcccgctgc cagctgccat ccgtgggggg agccgggtac ccgacgtcga cggacgtgat
     1081 ctccgacccg tcggggttct tcccggtggc gatgttcttc ggcacctcga tgaagtggcc
     1141 gctcacggtc tgggccgagc cggggacgaa gtcgtagtag tcgtcgaagc tcgggtcatc
     1201 ggagacgaac gcccagagat caccctcgtc ggcgagcagt gaatccgtgt ccggcgcgat
     1261 gtacgagtac agctgcgact gagaagccgc ggacgtctcg gtgttggggc cgcccggcgg
     1321 aatcgttagg gggccgctgg tgaacgtgtc gtcgcccgag agcacgacca ggtcgtcgaa
     1381 cccggggatc gcgacgctgt tctcgtggtt gtgccggccc atcccgtaga tgacgtggta
     1441 ctgcctgctc ttgacgtcga gcgcgagcac gacgccgctc tcctcctcgt ccggatcacc
     1501 gatcggaggc ggccacgagt cctcctgccg gaacacgtag tcgggggatt cctcgttcgt
     1561 gaacaggatc tcgcggtcga acccctcctt gctggtcgcg aggaagttcg agcagaaccg
     1621 ctggaacccg ccgctgctcg ggatcacgaa cgagccgtcg agcacgccgg cggagttccg
     1681 gttcaggatc agatggctca cctgcgaatt gtcgaagtcg gactccccat tggccgccgt
     1741 tggggtggtc gtgttgaacg ggaacggcac cttgctcgtt tcgtggttca cgtacacgtc
     1801 gacccgcccc tgcccacggg tgcgcaggga gatcccgtcg gggatggcct cgaagcggaa
     1861 gccgctctcc ccgaatacgt cccccacggt caggagcggc gtgatctgga caccagcttt
     1921 gacggcggtg agcatcgagg gtcgcccggt cttgaaccct ccgtttgagg ctgcgagcga
     1981 ggtcgtcgcc gtagccgtca gagctacggc gaggaacgtg ctgacgagga tcctccgtct
     2041 ctgcatgctt cccccttcgt gaccgcggac tcgaaccagt cctaacaggt tccgctcgaa
     2101 atgggcaacc cacgtcctgc gtggaaggat ctcctcgccg ccacgcgttc acctgcatgt
     2161 cgacgttccg tcatgaacga cacgacgaaa gagcctccac tcgacggtga cgtgcacgac
     2221 gtcagggtgc gggctcgcca tggcgctcga tagggtgatg catgcgcacg aacctgaccc
     2281 tcgaggacct tccgggcttc ctcgacctgc cgctggtcgc ggtcctcgcg accctgcgcc
     2341 aagacggttc ggtccagctc tcgccggtct ggcacgagtg gcgagacgct gggttcaacc
     2401 tgtggctccc ggcagcggac gtcaagaccc gtcatctgcg acgagatccg agggccagcc
     2461 tcgtcgtggc cgagtccgag acccccctcc ggggcgtcga gcttcgcacc gaggccagac
     2521 tcatcgaccg ggacgtccgc gccaccgccg tccggatcgc gacgcgctac gtgggtgggg
     2581 cgaagggaat cgagtacgtg gggtctgccg agaacgacgt gctcgcccgc ctcgaacccg
     2641 gccacctgcg ggtctgggac ttcgccgacg agtacgactg acactcgcgc acggccggca
     2701 tgtcgatcgc tccacggctc gggcggagat ggagggaagg aaagaagcac gtcctgggtt
     2761 gcgaagagga ggcgccctga gaccgcgatg gacagcccgg tgcgatgagt tcgcggtcct
     2821 tccggggtcg tagctggtgt gagcgaggga ggaggccatc gtgagtccat cgagcatctc
     2881 gatctccaag ctccgagcgg atgtcgacgg gcgggtgatc gcgcccgagg acgctgagta
     2941 cgaccaggcc cgagcgttgt tctacggggg gatggaccgc catccggccg ccatcgtccg
     3001 ggtcaaggac gacatggaca tcgttcgcgt cgttgcgctg gcacgcgaga gcggactcga
     3061 gctcgccgtt cggagcggcg gccacagcat cccgggccac agcgtctccg agggcgggat
     3121 cgtgatcgac ctcgccgaca tgcgggcgct cgatatcgat gtggaagcac gaacgacatg
     3181 ggcccagacc gggctgaccg ccctcgagtt cacgaccgcc gccgcggagc acggcctcgg
     3241 tgtcgggttc ggcgatactg gctcggtcgg gatcggcggg ctcacgctcg gcggcggggt
     3301 aggctacctc gtccgaaagt acggcctgac catcgacgac ctgctggccg ccgacatcgt
     3361 gaccgcggac ggcgagatcc accatgtcga cgccgagcac gagccggacc tgttctgggc
     3421 gatccgaggc gggggaggga acttcggcgt cgcgacgcgg ttcaggttcc gcctgcacga
     3481 gatcgacacg gtggtcggcg gcatgctgct tctccccgca accgtcgaca cgatcgccgg
     3541 cttcatggcc gaggccgagg ctgcgcccga ggagctctcg tcgatcgcca acatcatgaa
     3601 ggccccgccg atgccgttcg tccccgaaga agcccacggc aagctggtca tcatggcgct
     3661 catgacgtac gccggagacc cggaggccgg ccagggcgcc ctcgcgccgt tccgagccct
     3721 cgcggagccg gtcgccgacc tgctgcggcc gatgccgtac ccggaggtct acccgccgga
     3781 ggaggaggag taccaccccg tggccacggc gcgaacgatg ttcgtcgaca cgatcggccg
     3841 cgccgaggcg gagacgatcc tggaacacct cgcgacctcg accgcgatga tgggcgtagc
     3901 gcagctccgg atgctgggcg gagcgatggc ccgcgtgccc gcgaacgcga ccgcgttcgc
     3961 gcaccggcag agcaagatca tggtgaacgt cgccgccctc tacgagcgtc cggacgaggt
     4021 ggccgtgcac ggtccctggg tcgaggcgtt cgcggctgcg ctccgccagg gggacgacgg
     4081 tgcgtacgtc aacttcctcg ccgacgaggg cgaggcccgg atccgcgccg cgtacccggg
     4141 caccacatgg gatcgcctcc ggcagatcaa ggttcggtac gatccgacga acctgttccg
     4201 gctcaaccag aacatcccgc ctgcggtgga gggcttctcc tgaacacggt aacgtcgaag
     4261 gacggcaccg cgatcgcgtt cgatcgctcg ggcgacggac cacccatcgt gatggtgggc
     4321 ggtgcactca gcacgcgacg ggcggccgag acgctggcgg ggctcctcgc gccgagcttc
     4381 accgtctacg cgtacgaccg ccggggccgg ggcgacagcg gcgacatgcc gccgtacgcg
     4441 gtcgagcgcg aggtagagga catctactcg ctggtcgagg atgcgggcgg gagcgcggtc
     4501 gcgttcggtc actcctccgg cggcgtcctc gccctcgagg cggcggcaca tatcctcggc
     4561 atcaccaagg tcgcgctgta cgagccgccg ttcatcgtcg acgcctcgcg tgctccgttg
     4621 ccagacgact acatcgagcg gctcaccgag ctggcctcca cggaccgacc aggggacgcg
     4681 gtcgaatact tcatgatcac cggacccggc gtgccagccg aggctgtcgc atcgatgaag
     4741 gacgctccgt tctggtcggc gctggaggcc gtggcgcaca cgctgtcgta cgacgggagg
     4801 atcatggggg accagatggc gggcacgtcg ctgccctccg accggtgggc gtcggtcacg
     4861 atccctacgc tggtgatcga cggaggcgcg agcccgctgt ggttggggaa cgccgcgcag
     4921 gggctcgtcg acgcgctgcc cgacgcgcgg cgacggacga tcgagggcca gacgcacgac
     4981 gtcgatccgg tgctgctcgc cccggtcctg gaggagttct tcgccggctg aacggacctc
     5041 gaggaggcac aggtggacga tctcgcagtc gtcgcgaggg ggatcatcga cggcaaccgg
     5101 tacatggtgc tcgggacctc cgacgcggag ggacggccgt gggtctcgcc ggtgtacttc
     5161 accccgtcgg ggtacacgga gctctactgg gtctcctctc ccgaggcgaa ccactcgcgc
     5221 aacctcgccc gccgacccga gctgagtatc gtggtcttcg actcccgcgt ggccgtgggt
     5281 gaaggtcagg gggtgtacat gtcggccatc gcgcaggagc tgacgggcgc cgacctcgag
     5341 cgcggcatcg agatcttctc ccgcgtgtcg gagtcgcacg gcgccaagcc atggacggtc
     5401 gacgatgttc ggcctcccaa gccgtatcgt ctctatcgcg cgcgcgtgtc cgagcactgg
     5461 gtcctggacc ccgagagtcg tcccgaccaa cggacgccgg tgcaaccgtg agaacggcgc
     5521 cgctaggttg aggacatgac cgggcggatc acggcacccg gagggcaacg aggccgacgt
     5581 gtgtacatgg cgaagcacgg gctgagggat cttgcgcggt cgacgcagct acgatgcccg
     5641 gatggcccga acacccgagc agctgtaggg tccgatcgcg aagacccagc tcgcaacgcg
     5701 cggggtcacc gccggcacgg gcttcgggaa taccgagggg ctgaggatct ccgggaagat
     5761 cttcgcgatg ctcgttggag acgagctcgt cgtgaagctc ccgaaggaac gggttgacga
     5821 gctcgtcgac gccggcgtcg cgaaacggtt cgacgcgggg aagggccgcc cgatgaagga
     5881 gtgggcttcg atcgctccga gcgcttcccg gcgttggagg ggtctggtcg acgaggcgag
     5941 agccttcgtc ggcggtcgtt gatgtcggag agatcggcgt ggagctcggt cacgaggatc
     6001 aacgatcat
//
LOCUS       DASUYD010000218         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_810744, whole genome shotgun sequence.
ACCESSION   DASUYD010000218 DASUYD010000000
VERSION     DASUYD010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_810744"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1451)
                     /locus_tag="VF195_11560"
     CDS             complement(<1..1451)
                     /locus_tag="VF195_11560"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HEX6581494.1"
                     /translation="MTTPSEATVHRSVALVWRTLRSMRTALILLLILAAGAVIGSLLP
                     QVPNSPERVGRYLDDHGVWGTFLFRAGFFDVYGSWWFGLITTLLFVSLAACLFPRTRA
                     MVRALRQRPVQARELDAFRHHAEMRVEHDPDAVAADAARHLRRKRFRVTRQGDGVAAE
                     KGVLREVGSLLFHWAFFLLLVGVIVGKGTGFTGRAVVTESETWIDARANYDGEIRTGR
                     YFGGAHTGIGIELLDFEDSYRRNGLPIDFVSRLRFRDRDGSPLGVEEIRVNHPTSRDG
                     IRIFQEGFGWAPVVTAALDGESMWSSPIEMTRDEPPDGVPATAMPWRGAIKLTAPEPD
                     VMISLELWSDYRAFANLQLTGRPTPMLVQFDPFIRYSVYVGRIADPARSSIDTTGLRR
                     VARGDLHAAGSDRTEVSGVGQLTLSFPELRQYSVLLISKDVGIPMVLAAAILVLVGLI
                     PALYVSRRKVWVRAEAAPGGALVKIGGFALQRK"
     gene            complement(1475..2200)
                     /locus_tag="VF195_11565"
     CDS             complement(1475..2200)
                     /locus_tag="VF195_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX6581495.1"
                     /translation="MMGGLVDTGLSSWWAPALAFAAGVVSFASPCVLPLVPGYVSFVT
                     GSRATEGEGRRPLVPILLFILGFTIVFTLLGAFAGTFVPFFRDPWFQRVAGLMVLGLG
                     LLMLGYAFRRGSPGLYSERRPLLERVRPGRAGALPLGIAFAAGWTPCIGPVLAGILVI
                     AASGGTVKGAALLVVYSLGLGLPFLLVGIGADRLVRSLGWVKRHYRVMAGVSGALLVV
                     VGVLLMTGLFRQVFLPLSRFAWL"
BASE COUNT          435 a          878 c          859 g          296 t
ORIGIN      
        1 tccttccgtt gcagcgcgaa gcctccgatc ttcacgaggg cgccgccggg ggcggcctcc
       61 gctcggaccc agaccttccg tcgggagacg tacagcgcgg ggatgagtcc cacgagaacg
      121 aggatagcgg cggcgaggac catcgggatg ccgacgtcct tgctgatcag gaggaccgag
      181 tactgccgga gttcagggaa cgacagcgtc agctgaccca ccccggacac ctccgtccgg
      241 tcgctgccgg cggcgtggag atcgccgcgc gcgacccgcc ggaggcccgt cgtgtcgatc
      301 gacgatcgcg ccggatcggc gatccggccc acgtacaccg agtatcggat gaaggggtcg
      361 aactggacga gcatcggcgt gggccggccg gtgagctgga ggttcgcgaa cgcgcggtag
      421 tccgaccaga gctcgaggct gatcatcacg tccggctccg gtgcggtgag cttgatcgcg
      481 ccgcgccacg gcatcgcggt cgcgggcaca ccgtccggcg gctcgtcgcg cgtcatctcg
      541 atcggcgagg accacatcga ctcaccgtcg agcgcggccg tcaccaccgg ggcccagccg
      601 aacccctcct ggaagatccg gatcccgtcc cgggacgtgg gatggttcac gcggatctct
      661 tccaccccca gcggcgatcc gtcccggtcg cggaaccgga ggcgagagac gaagtcgatc
      721 ggcagtccgt tccgccggta gctgtcctcg aagtccagca gctcgatccc gatccccgtg
      781 tgtgctcccc cgaagtagcg ccccgtccgg atctcgccgt cgtagttcgc gcgcgcgtcg
      841 atccacgtct cgctctcggt cacgacggcg cggccggtga agccggtccc cttgcccacg
      901 atcaccccga ccaggaggag gaagaacgcc cagtggaaca ggaggctccc gacctcgcgg
      961 agcacgccct tctccgcggc gacaccgtcg ccctgccggg tgacgcggaa gcgcttccgt
     1021 cgcaggtggc gggccgcatc cgccgcgacc gcgtcggggt cgtgctcgac ccgcatctcg
     1081 gcgtgatgcc ggaacgcgtc cagctcccga gcctggacgg gccgctgccg gagggcgcgc
     1141 accatcgctc gggtcctcgg gaacaggcac gccgcgagcg agacgaacag cagcgtcgtg
     1201 atgagcccga accaccagga cccgtagacg tcgaagaagc ccgcgcggaa gaggaacgtc
     1261 ccccagacac cgtggtcgtc gagatagcgc ccgacccgct ccggcgagtt ggggacctgg
     1321 ggcaggagcg atccgatcac ggccccggcg gcgaggatca acaggaggat caacgccgtc
     1381 cgcatcgagc gcaacgtgcg ccacacgagc gccacggatc ggtggacggt ggcttcggac
     1441 ggcgtcgtca tggcgtccgg atgctagccc gcacctacag ccacgcgaac cgcgacagcg
     1501 gcaggaagac ctgccggaac agtcccgtca tgagcaggac gcccaccacg accaggagcg
     1561 cacccgagac ccccgccatc acccggtagt ggcgtttgac ccacccgagg ctgcggacca
     1621 ggcggtccgc cccgatcccc acgagcagga acgggagacc gagaccgagc gagtacacga
     1681 cgaggagggc ggctcccttc accgtgccgc cgctcgccgc gatcacgagg atgcccgcga
     1741 gcaccggccc gatgcacggg gtccagccgg ccgcgaacgc gatcccgagc ggcaatgccc
     1801 cggcccggcc ggggcggacc cgctcgagga gcggccggcg ctccgagtag aggcccgggg
     1861 acccccggcg gaacgcgtac ccgagcatca gcagaccgag cccgagcacc atcagccccg
     1921 cgacgcgctg gaaccaggga tcgcggaaga acgggacgaa cgtcccggcg aacgcgccga
     1981 gaagggtgaa cacgatcgtg aacccgagga tgaacagcag gatcggcacg agcgggcgcc
     2041 ggccctcgcc ctcggtcgcg cgactcccgg tgacgaacga gacgtagccc ggcaccaggg
     2101 gaaggacgca ggggctggcg aaggacacga cgccggccgc gaaggcgagg gccggcgccc
     2161 accacgacga gagccccgtg tcgaccagtc cccccatcat cgtgctcagt ctcccaccgg
     2221 tcgcgcgagc ggttcgggtt gtctgtgccc tggtcgcgac atagtgtgga ggcgctcgtg
     2281 aaaaccttca cgagtgggag gcgggggccg agccgcgacg cgaagccctc cgaggcccgg
     2341 ttgtatgatt cgcccgccca tggagcaagc gagcgcatga gccaggtcga gaccgtagcc
     2401 agatcgccga tccagaccgt caagcgccat gtcgagtcgt tcctgcggga cttcgagtgg
     2461 tcgtggac
//
LOCUS       DASUYD010000219         9083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_151632, whole genome shotgun sequence.
ACCESSION   DASUYD010000219 DASUYD010000000
VERSION     DASUYD010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9083
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151632"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF195_11570"
     CDS             complement(<1..739)
                     /locus_tag="VF195_11570"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6581496.1"
                     /translation="MVDEPAALRKPRSPGRRATSIVRRLAGKNVFVTGVTGFLGQAVF
                     ERLLLDFPETRITLLVRPQLGSSGRQRVESLMGRPTFNALRDKVGTEGIAAMLAERVD
                     VVDGDFSAAELEIPGGLDVVIHCAATVAFDPPIDEGFLTNLVGAVRLYEAALANNPSP
                     HLLHVSTAYVAGVRKGVIPEATLDHRVDWEAESDLAIGARADVEAASRKPEILDAFLR
                     KARSEHGRAGPQSVADDAEERRKTWVTK"
     gene            complement(785..2170)
                     /locus_tag="VF195_11575"
     CDS             complement(785..2170)
                     /locus_tag="VF195_11575"
                     /inference="COORDINATES: protein motif:HMM:NF023401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEX6581497.1"
                     /translation="MKMTLLGSGVRAPFVLKGLADEHDALGLDEVVLHDTDSDRLELM
                     TALGSHLCAEWGTGFTVRGEPNPEAALADARFVFSAIRPGQEGARVVDEELPLKHGVL
                     GQETTGPGGFAMALRTVPAMLGYARLIEDVAPGALLVNFTNPVGIVMQALHDRSSVDA
                     VGVCDGPVAMQESVARFLGLSRDRVHADYFGLNHCGWIHRVLVDGHDRLPELLDRFGE
                     LQATDEQWMLFDPPLVREIGMLPMEYLYFYYYREQALDHIRGSGSSRARQLLTLNDAL
                     WPVLRARIDAGDLSGAQAAWERAMVERGETYFARERGQPLEEEVLEAGDIFEGEGYEG
                     VATAVMAAVVERRKATLILNVPNRGAIGGLRDDDVVEVTCMMDEHGAHPIAQGQMPDA
                     ALALVAQVKLYERLAVSAALEGSYDTALDAMLAHPLVASYPIAKALLDGYMDELGELL
                     PPLKGSERLAP"
     gene            complement(2167..3129)
                     /locus_tag="VF195_11580"
     CDS             complement(2167..3129)
                     /locus_tag="VF195_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX6581498.1"
                     /translation="MSSAFDLLVCGRPSVDVMFSGLDGSPALGEDIEADGLGVCAGTS
                     FNTPAAANRIGLRVAYVATIGNDMWSRMIRDEFEAEGLPTDYLEVEDRPLPGISVAFN
                     YGGDRGFITHWGSGERYDERLRVRALEVAGTVEARHVHAYVDDMPELEAIAHRRGMTV
                     SLDAWGGAAWSSSRPVPEILARADVLLANEAEAAAMTGEAEPDRALETLAEHCPCVVI
                     KLGAAGAIGSAEGHRETVPADPMKIVDTTGAGDSFNAGFLAGWLGGLALEHSLALGVI
                     CGSRAVGDYGGYRGCPGKDELRAIARSRGISLRFGRADEGARRT"
     gene            complement(3126..3857)
                     /locus_tag="VF195_11585"
     CDS             complement(3126..3857)
                     /locus_tag="VF195_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEX6581499.1"
                     /translation="MGGPRTVVVENADELTRAAADVVSDVIVGKPEASVVAATGRTPM
                     GLYAELAARHEKGLRTAGITAFQLDEYLGLGPTDRRSLFGWMRQSFLEPLGIHDERAV
                     RLPTEGDVEGSCEEFDAAIRARGGLDLAILGLGPNGHLGFNEPPSGPDDPTRIVTLSP
                     ATVEANARYWGDIADVPTDAVTMGLQQLLAVRTIVLIASGRGKRDIVHAALEGPVAPE
                     VPASFLQVTAAEVTAFVDRDAWGEA"
     gene            complement(3862..4608)
                     /locus_tag="VF195_11590"
     CDS             complement(3862..4608)
                     /locus_tag="VF195_11590"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX6581500.1"
                     /translation="MSNAGGGPVLEKLQLRYLRVQQALAAEIARSRWDPGFLLPPERA
                     LAEHFGVSRVTLRRALAELEREGVVTRNGARWTVGSSPIGEPPNVLMSFSEMAGTRGL
                     RASAAVIGARRRAATLDEADTLGLAPGAALFELERVRALDGIPILLDRSCLPASLVEG
                     IEGTDFSSASLYEMLEERYDVRPTRARFTIDAIAADDRRARLLELEPGDPLLRCLQQT
                     EDGTGRLIELCEMVYRGDRYRFHATLVRGG"
     gene            4701..6077
                     /locus_tag="VF195_11595"
     CDS             4701..6077
                     /locus_tag="VF195_11595"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX6581501.1"
                     /translation="MRAGRHSIVARSRLFAFVVIAGLIAMACTPGGSTPEASPPPPPS
                     RTGITETEPITLTVWDQECCRVSKVWDQLNAEFEAEYPNLTIERVNRDFGELKTLLRL
                     AIAGPKGPDVVEVNQGWPDMGQLVKAGLLLPLDNYAESYGWTERVSENVLAVSRWTPD
                     GEQFGTGNLYGYTTFGEIIGVYYNAGMLDDLALTLPTTFDEFEQALESAKQAGEVPIQ
                     FGNLDAFPGIHEYVVIQDRMASVDYVTDLVFGLQGNDLSFDTPENLQAATTLQEWAAK
                     GYFTPGFGGGGYDDAVAEFAGGEGLFMITGNWIVESLGPDSREFGFFVMPPMQAGDPQ
                     VSTGGAGFPLSIAAGTEHPDAAAAYIDWMTDDHAADLLLPTGQIPLSIGATVSVQDGT
                     VLADVVDAAGAVSDANGMVPYLDWATPTFYDTTTASIQELMALRITPQELVQEIEADY
                     SGFQSSRA"
     gene            6077..7027
                     /locus_tag="VF195_11600"
     CDS             6077..7027
                     /locus_tag="VF195_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020500433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX6581502.1"
                     /translation="MDAEPPVASRAVVVGSRRKPKAERLPGEARRIGYLYVAPAFILY
                     AAFNLFPLGQGVNLSFYDWDGITPGTWVGLDNYLEFFTEPAIREGYAHVLQLMVFYSF
                     LPIVLGLFLAALLSRIRIRGLTFFRVLLFLPLVITDVVTSVAWIWIYDVDGPLATAME
                     AVGLGALVPSAGWLGDFNTALPAVGVFGLWGQFGFCLILFLAGVMKIPASLYEAARVD
                     GANAVREFFAVTLPGLRYELQVVLVLTVIGTLGTFDEIYVMTGGGPGTSTSVPAYQIW
                     SRLFRTGEVGSAAALGLVLMLIVFAVTFAINRLMERGAER"
     gene            7024..7851
                     /locus_tag="VF195_11605"
     CDS             7024..7851
                     /locus_tag="VF195_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012936895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX6581503.1"
                     /translation="MTPTSRSQITMNYVLLILVSAIAVYPLVGVLFASFYPSGPGTSP
                     SGFGLPPSLEWRNYTTAWEQGGFAESFTTSVIVAAVMVPASVILSILGGYAFATMRFP
                     GSKAIFYVFILGLIIPTEATIVPLYYDVRALGLIDTYWALIILEVSGEVAFGIFWMRA
                     AFLSAPASLLEAARIDGASSWGALWGIMVPFAKPAILTLTVLVFADSWNEFFLALVFT
                     INHPTAPAGLASFSGRFTTDIALVSAGAVIVALPIVIVFIIFQREFMRGMLTGAVKG"
     gene            7855..8949
                     /locus_tag="VF195_11610"
     CDS             7855..8949
                     /locus_tag="VF195_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581504.1"
                     /translation="MAQVVYDHVVKTFGEVTALHDLSLEISDSEFVVLVGPSGSGKTT
                     ALRLIAGLETLTGGSIRIGERTVDRVAPRDRDIAMVFQDYALYPQKTVYQNLAFGLRM
                     RKMPKHEIDLRVRRAAEMLDIGPLLQRRPGALSGGQRQRVALGRALVREPRVFLMDEP
                     LSNLDAQLRVQTRSEIKHLQEQVGTTTVYVTHDQVEAMTMGDRVAVMSEGRLEQVGDP
                     RTIYDEPANVFVAGFIGSPAMGFWTMRAERTPGALGLIADGTALEVAINPDVPELPPE
                     VVVGVRPEHALIWRENAGLVGPVEGRVNYVEMLGRESFIGIETAGEARITVRAEPDVA
                     VRPGERVPFGFEPGRLHLFDPKTERTIARL"
BASE COUNT         1439 a         3126 c         3020 g         1498 t
ORIGIN      
        1 gcttggtcac ccaggtcttg cgccgttcct cggcgtcgtc tgcgacggac tgggggcctg
       61 cgcgtccgtg ctcggagcgg gccttgcgca ggaacgcgtc gaggatctcg ggcttgcggc
      121 tcgcggcctc gacgtcggcc cgcgccccga tcgcgagatc ggactccgcc tcccagtcaa
      181 cgcggtggtc gagcgtggcc tcggggatca cgcccttgcg cacccccgcc acatacgcgg
      241 tcgagacgtg gagcaggtgc ggggaggggt tgttcgcgag cgccgcctcg tacagacgga
      301 cggcgccgac caggttggtg aggaagccct cgtcgatggg cggatcgaac gccaccgtgg
      361 cggcgcagtg gatcacgacg tcgaggcccc ccgggatctc gagctcggcg gccgagaagt
      421 ccccgtcgac gacgtccacg cgttcggcaa gcatcgccgc gatcccctcg gtgcccacct
      481 tgtcccgaag cgcgttgaac gtgggacggc ccatcaggga ctcgacccgc tgacggccgc
      541 tcgaaccgag ctgcggccgc accagcagcg tgatccgcgt ctcggggaag tcgagcagga
      601 ggcgttcgaa cacggcctgc cccaggaagc cggtcacgcc cgtcacgaag acgtttttgc
      661 cggcgagcct gcgcacgatc gaggtcgcgc gcctgcccgg gctccgaggc ttccgcaggg
      721 ccgcgggttc gtctaccacg tggcagatta tgccctatct accgagtggt agacaagggt
      781 caagctacgg ggcgaggcgc tcggacccct tcaaaggtgg aagcagctcc cctagctcat
      841 ccatgtatcc gtccaggagc gccttcgcga tcgggtacga ggccacaaga ggatgcgcga
      901 gcatcgcatc cagcgccgtg tcgtacgacc cctcgagcgc ggccgaaacg gccagccgct
      961 cgtacagctt gacctgagcg accagcgcca aggcggcgtc gggcatctga ccctgggcga
     1021 tcggatgagc tccgtgttca tccatcatgc aggtgacttc gaccacgtcg tcgtcgcgga
     1081 ggccgccgat cgctccgcgg ttcggtacgt tcaggatcag ggtcgccttc cggcgctcga
     1141 cgaccgcggc catcaccgcg gtcgcaacgc cctcgtaccc ctcaccctcg aagatgtcgc
     1201 cggcctcgag cacctcttcc tccagcggct ggccgcgctc gcgcgcgaaa taggtctcac
     1261 ctcgctccac catggctcgt tcccacgccg cctgcgcgcc cgagaggtca ccggcgtcga
     1321 tgcgtgcgcg cagcacgggc cacaaggcgt cgttcagcgt caggagctgc cgagctcgcg
     1381 agctccccga accacggatg tggtccaggg cttgttctcg gtagtagtag aagtagaggt
     1441 actccatcgg cagcatgccg atctcgcgca ccaacggggg atcgaacagc atccattgct
     1501 cgtcggtggc ctggagctcg ccgaatcggt ccaacagctc gggcaaacga tcgtgcccgt
     1561 ccaccagcac cctatggatc cacccgcagt gattcagccc gaagtaatcg gcatggaccc
     1621 gatcgcgaga cagtccgagg aaccgcgcga cgctctcctg catcgcgacg gggccatcgc
     1681 atacgcccac cgcgtcgacc gacgagcgat cgtgcagcgc ctgcatcacg atcccaacgg
     1741 ggttcgtaaa gttcacgagc agcgcgcccg gcgcgacgtc ctcgatgagg cgcgcatagc
     1801 cgagcatcgc ggggaccgtc cggagcgcca tcgcgaaacc tccgggacca gtcgtctcct
     1861 gtccgagcac gccgtgtttc agcggcagct cctcgtccac gacgcgtgcg ccctcttgcc
     1921 cgggacggat cgcggagaag acgaatcggg catcggcgag ggcggcctcg gggttgggtt
     1981 cgccgcgcac agtgaacccg gtcccccact cggcgcagag gtgacttcct agtgcggtca
     2041 tcagctcaag ccggtcggaa tcggtgtcgt ggaggacgac ctcgtcgagc ccgagtgcgt
     2101 cgtgctcatc tgccaaaccc ttcagcacga atggcgcccg aacacccgag ccgagcaacg
     2161 tcatcttcat gttcgtcgcg ctccctcatc agctcgcccg aagcggagcg agatccctcg
     2221 cgatcttgcg atcgcgcgaa gctcgtcctt cccggggcag ccgcgatacc cgccgtagtc
     2281 gccgaccgcg cggctcccgc agatgacccc gagcgctagc gagtgctcca gtgccagacc
     2341 gccgagccac ccagcgagga acccagcgtt gaagctgtcg cctgccccgg tcgtgtcgac
     2401 gatcttcatc ggatccgccg gcacggtttc ccgatgccct tcggcggagc cgatcgcccc
     2461 cgcggctccg agcttgatca cgacgcatgg acagtgttcg gcgagggtct cgagagcccg
     2521 gtccggctcc gcctcaccgg tcatcgccgc cgcctccgcc tcgttggcga gcagcacgtc
     2581 ggccctggcc aggatctcgg ggacgggccg ggacgacgac caggcggcgc ctccccacgc
     2641 atccaacgag accgtcatcc cgcgccggtg ggcgatcgcc tcgagctcgg gcatgtcgtc
     2701 cacgtacgcg tgaacgtgcc gtgcttcgac cgtgccggca acctccaacg ctcggaccct
     2761 gagtcgctcg tcgtagcgct caccggagcc ccagtgcgtg atgaagcccc ggtcgccgcc
     2821 gtagttgaac gccaccgaga tgccgggcag cggccggtcc tcgacctcca ggtagtcggt
     2881 cggcagcccc tccgcctcga actcgtcgcg gatcatccgg ctccacatgt cgttgccgat
     2941 cgtcgcgacg taggcgaccc gcaggccgat ccggttcgcg gcggccgggg tattgaacga
     3001 cgtgccggcg cacacgccca ggccatcggc ctcgatgtcc tcgccgagcg ccggcgaccc
     3061 atccagcccg gagaacatca cgtccactga ggggcgcccg cacaccagca ggtcgaacgc
     3121 cgaactcatg cctcccccca ggcatcgcgg tcgacgaagg ccgtcacctc ggccgccgtc
     3181 acctgtagga aggaggcggg gacctcgggc gcgaccggtc cctcgagggc cgcatggacg
     3241 atgtctcgct tgcctcgtcc ggatgcgatc aggacgatcg tccgcacggc caggagctgc
     3301 tgaaggccca tcgtcacggc gtccgtgggg acgtccgcga tgtcgcccca gtagcgagcg
     3361 ttcgcctcga cggttgcagg ggacaacgtg acgatgcgcg tgggatcgtc ggggcccgag
     3421 ggcggctcgt tgaagccgag gtggccgttc ggtcccagac cgaggatcgc gaggtcgagg
     3481 ccccctcgtg ccctgatcgc agcatcgaac tcctcgcatg atccttcgac gtctccctcg
     3541 gtcggcagcc gcaccgctcg ctcgtcatgg atcccgagcg gttccaggaa gctctgccgc
     3601 atccaaccga acagtgagcg gcggtcggtc ggcccgagac ctaggtactc gtcgagctga
     3661 aacgccgtga tgccggccgt gcgaagaccc ttctcgtggc gtgctgcgag ctcagcgtac
     3721 aaacccatcg gggttcgacc ggtcgccgcc accaccgagg cctcgggctt ccccacgatc
     3781 acgtcgctca cgacgtcggc cgcggcccgc gtgagttcgt cggcgttctc gacgaccacc
     3841 gtgcgagggc cgcccatccc gtcagcctcc gcggaccagc gtggcgtgga agcggtagcg
     3901 gtcgcctcgg tagaccatct cgcacagctc gatcagccgg cccgtcccgt cctcggtctg
     3961 ctggaggcat cgaagcaagg ggtctccggg ctcgagctcg agcaacctgg ctctgcggtc
     4021 gtcggccgcg atcgcgtcga tggtgaagcg tgcgcgtgtg ggtcgcacgt cgtaccgctc
     4081 ttcgagcatc tcgtagagtg aggcgctcga gaagtccgtt ccctcgatcc cttcgactag
     4141 cgatgcggga aggcagctcc gatcgagcag gatcgggatg ccgtccaacg cccgcacacg
     4201 ttcgagctcg aacagggccg ccccgggtgc cagtccgagc gtgtccgcct cgtcgagcgt
     4261 cgcggctctg cggcgggcgc cgatcacggc tgcagacgct cgcaggccac gtgtcccggc
     4321 catctccgag aagctcatca ggacgttcgg cggttctccg atgggggagc tccctaccgt
     4381 ccacctcgcc ccgttgcgcg tgacgacgcc ctcgcgctcg agctcggcga gagcccggcg
     4441 cagcgtcacg cgactgacgc cgaaatgttc tgcgagcgcc cgctcgggcg gaaggaggaa
     4501 gcccggatcc caacggctcc tcgcgatctc ggcggcgagg gcctgctgga cgcggaggta
     4561 acggagctgc agcttctcga ggaccggccc gccccccgcg ttcgacatgc acgggatgat
     4621 agaccaagct caacccgaaa gaaaccaatc acagaccaat ggtattgact cggtcgacct
     4681 ccgggcctag agtcacccgc gtgagggctg ggcggcactc catcgtggcg cgatcgcgac
     4741 tgttcgcctt cgtcgtcatc gccggcttga tcgcgatggc atgcacaccg ggtggctcga
     4801 caccggaggc ctcaccgccg cccccgccct ccagaaccgg gatcaccgag acggagccga
     4861 tcacgctcac cgtttgggac caggagtgct gtcgggtcag caaggtctgg gatcagctga
     4921 acgcggagtt cgaggcggag tatccgaacc tcacgatcga gcgggtgaac cgggacttcg
     4981 gcgagctgaa gaccctgctg cggctggcca tcgcgggacc gaaaggtccg gatgtggtgg
     5041 aggtgaacca ggggtggccg gacatggggc agctcgtgaa ggccgggctc ctcctgccgc
     5101 tcgacaacta cgccgagtcc tacggctgga ccgagcgcgt gtcggagaac gtgctggccg
     5161 tcagcaggtg gacgcccgac ggcgagcagt tcgggacggg gaacctctac gggtacacga
     5221 ccttcggcga gatcatcggc gtctactaca acgcggggat gcttgacgat ctcgcgctga
     5281 cgctcccgac gaccttcgac gagttcgagc aggcgctcga gtcggccaag caggcaggcg
     5341 aggtgccgat ccagttcggt aacctcgacg cgttccccgg gatccacgag tacgtcgtga
     5401 tccaggaccg catggcctcg gtggactacg tcaccgatct cgtgttcggg ttgcagggca
     5461 acgacctttc gttcgacacc cccgagaacc tccaggccgc gaccacgctg caggagtggg
     5521 cagcgaaggg gtacttcacc cccgggttcg gcggtggggg gtacgacgac gcggtcgcgg
     5581 agttcgcggg cggcgagggc ctgttcatga tcaccgggaa ctggatcgtc gagagcctcg
     5641 ggcccgacag ccgggagttc ggctttttcg tgatgccgcc gatgcaggcg ggagaccccc
     5701 aggtctcgac gggcggggcg gggttccccc tctcgatcgc cgcgggcacc gaacatcccg
     5761 acgccgcagc cgcgtacatc gactggatga cggatgacca tgccgccgac ctcctcctgc
     5821 cgacgggtca gattcccctg agcatcggcg cgacggtctc ggtgcaggac gggacggtgc
     5881 tcgcggacgt ggtcgacgcg gcaggcgccg tgagcgatgc caacggcatg gtcccctacc
     5941 tggactgggc gacacccacc ttctacgaca cgacgacggc gtcgatccag gagctgatgg
     6001 cgctcaggat cacgccccag gagctcgtcc aggagatcga ggcggactac tcgggattcc
     6061 agagctcgcg agcctgatgg acgcggagcc tccggtcgcc tcgagagcgg tcgtggtcgg
     6121 gagccgacgg aaaccgaagg ccgagcgcct tccgggggag gcgcggcgga tcggctacct
     6181 gtacgtggcc cccgcgttca tcctctacgc cgcgttcaac ctgttcccgc tcgggcaagg
     6241 ggtcaacctg tccttctacg actgggacgg gatcacaccg ggcacctggg tgggcttgga
     6301 caactacctc gagttcttca ccgagccagc gatccgggag ggatacgccc atgtgctcca
     6361 actcatggtg ttctactcgt tcctgccgat cgtccttggc ctgttcctcg ccgcgctcct
     6421 ctcgcggatc cggatccgcg ggctcacgtt cttccgtgtg ctgctgttcc tcccactcgt
     6481 catcaccgac gtcgtcacgt cggtggcgtg gatctggatc tacgacgtcg acggacccct
     6541 cgcgaccgcg atggaggccg tggggctcgg agccctggtc ccgtcggccg gctggctggg
     6601 cgacttcaac acggcgcttc ccgccgtagg cgtcttcggg ttgtggggac agttcggctt
     6661 ctgcctgatc ctgttcctcg ccggggtgat gaagatccct gcgagcctgt acgaggcggc
     6721 tcgggtcgac ggagccaacg ccgttcggga gttcttcgcc gtgaccctgc ccgggctgcg
     6781 gtacgagctg caggtggtgc tcgtgctgac cgtcatcggt acgctcggaa ccttcgacga
     6841 gatctacgtg atgaccggcg gaggcccggg cacctccacg tcggtccccg cctatcagat
     6901 ctggagtcgg ctgttccgga cgggggaggt cgggtcggcc gccgcgctcg ggctcgtgct
     6961 gatgctgatc gtcttcgcgg tgacgttcgc gatcaaccgg ctcatggagc ggggggccga
     7021 acgatgacgc cgacctcccg atcgcagatc acgatgaact acgtgctcct catcctggtc
     7081 tcggcgatcg ccgtgtaccc gctcgtcggc gtcctgttcg cgagcttcta cccgtccggc
     7141 cccgggacgt cgccgtcggg cttcgggctt ccacccagcc tcgaatggcg gaactacacg
     7201 accgcgtggg agcagggggg attcgccgaa tccttcacga ccagcgtcat cgtggccgcc
     7261 gtcatggtcc cggcgtcggt gatcctgtcg atcctgggcg ggtacgcgtt cgcgacgatg
     7321 cggttcccgg gctcgaaagc catcttctac gtgttcatcc tcggactcat catcccgacc
     7381 gaggcaacga tcgtgccgct gtactacgac gtgcgggcgt tgggcctaat cgacacgtac
     7441 tgggccttga tcatcctcga ggtctcgggc gaggtcgcgt tcgggatctt ctggatgcgc
     7501 gcggcgttcc tgtcggcccc agcttcgctg ctggaggccg ctcgaatcga cggcgcatcg
     7561 agctggggag cgctctgggg catcatggtg ccattcgcga agccggcgat cctgacgctg
     7621 acggtgctcg tcttcgccga cagttggaac gagttcttcc tcgccctcgt gttcacgatc
     7681 aaccacccga cggcacccgc cggactcgcc agcttctccg gcaggttcac gaccgacatc
     7741 gccctcgttt ccgccggtgc ggtgatcgtg gcgctaccga tcgtcatcgt cttcatcatc
     7801 ttccagcgcg agttcatgcg cggcatgctg accggcgcgg tgaagggctg atcgatggcg
     7861 caggtcgtct acgaccacgt cgtcaagacc ttcggtgagg tcacggcgct ccacgacttg
     7921 tcgctcgaga tctcggacag cgagttcgtc gtgctggtcg gaccgtcggg gtccggcaag
     7981 accaccgcgc tgcgactcat cgcgggactc gagacgctca ccggcggctc gatccggatc
     8041 ggcgagcgga ccgtcgatcg ggtcgctcct cgtgatcgcg acatcgcgat ggtgttccag
     8101 gactacgccc tttatccgca gaagaccgtg tatcagaacc tcgccttcgg gctacggatg
     8161 cggaagatgc cgaagcacga gatcgacctg cgcgtccgcc gtgccgccga gatgctcgac
     8221 atcggaccgc tgttgcagag gaggccgggg gccctctcgg gcggccagcg tcagcgtgtt
     8281 gccctcggca gggccttggt gcgcgagcca cgcgtcttcc tgatggatga gccgttgtcg
     8341 aacctcgacg cgcagctgcg ggttcagacg aggagcgaaa tcaagcatct ccaggagcag
     8401 gtggggacga ccaccgtcta cgtgacgcac gaccaagtcg aggcgatgac catgggagat
     8461 cgtgtagcgg tcatgagcga gggacgcctc gaacaggtcg gcgacccgag gacgatctac
     8521 gacgagcctg ccaacgtgtt cgtggccggg ttcatcggaa gcccggcgat gggcttctgg
     8581 acgatgcgcg ccgagcgcac tcccggcgcg ctcggcctca tcgccgatgg gacggcgctc
     8641 gaggtcgcga tcaatcccga cgtgcccgaa cttccacccg aggtggtcgt cggcgtccgg
     8701 cccgagcacg ccctgatctg gcgggagaac gcgggtctgg tcggccccgt cgagggacgg
     8761 gtgaactacg tcgagatgct cgggcgagag agcttcatcg gcatcgagac ggcaggggaa
     8821 gcgcgcatca cggtccgcgc cgaacccgat gtcgccgtcc gtccgggcga gcgcgtaccc
     8881 ttcgggttcg agcccggccg cctccaccta ttcgacccga aaacggaacg aacgatcgcc
     8941 cgcctgtagc gcccggggcc cgatccggcg acaaggaccc accgacatcc ctaggctctc
     9001 ggtcgatgag aacgagcgct cagcaagcac ctccctggcg agcggtcttc gccggcgcgc
     9061 gagggaggct cacgatcggg ctg
//
LOCUS       DASUYD010000220         6604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_379285, whole genome shotgun sequence.
ACCESSION   DASUYD010000220 DASUYD010000000
VERSION     DASUYD010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6604
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379285"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1306)
                     /locus_tag="VF195_11615"
     CDS             complement(<1..1306)
                     /locus_tag="VF195_11615"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX6581505.1"
                     /translation="MKRRPMSPGDYRLVRTVGRLPARVHTKLLIAFVGTAVLLVAVGA
                     LGLRVLAQSDDRVSELGPLQERAAAYGKLQSDTSNVRNLLAENLGRNSDYFVVWPDDS
                     ALGVRVAVDQAIANQVRRIPATTFPDRLGFVPPAEDESVLREIREESDRLSVLMEEVI
                     RDPNEAVRAEAERLAEGLDQLASELANATTAKANVVIADNARAYESSRSLYVGVAVGA
                     IGLALVLGFVLSWSVVGPIQRIDSRLATIAAGDFSGHVDVENRDELGALAANVNRTND
                     ELKRLYAELEAASQHKSDFLANMSHELRTPLNAIIGFSQVLSEGMAGAVNEKQAEYLD
                     DILSSANHLLALINDVLDLSKVEAGQVELEKAPFSLREALERGVVVVRERASDDGVSV
                     ALDADPDADVVEGDERRIQQVIFNLLSNAVKFTPVGGAVDVTS"
     gene            complement(1356..1820)
                     /locus_tag="VF195_11620"
     CDS             complement(1356..1820)
                     /locus_tag="VF195_11620"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6581506.1"
                     /translation="MVVSLAACGDSGSDTETAALQLKADKYEIEQIEKVFHQAMTTKD
                     IDLMVSLWAPNATLTVGPEATAAGVDEIRQFWLEQSVAFAPETTWITDHPAYKMEITV
                     NGDRGTLHFECHFVNYQTGEIEAITGTDQDVARIDGRWLITNMVGASTELTL"
     gene            complement(1997..2371)
                     /locus_tag="VF195_11625"
     CDS             complement(1997..2371)
                     /locus_tag="VF195_11625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581507.1"
                     /translation="MPVVLIVDDNEKNRKLAGSVLSAAGFRTIEAATGPEALALAAKH
                     VPDVILMDLRLPDIDGTEAMRTLGEQERTARIPVVAVSAMRLEASGDWLEAAGFAGWI
                     EKPIRIDTFADQVRRYCKEATG"
     gene            2389..3054
                     /locus_tag="VF195_11630"
     CDS             2389..3054
                     /locus_tag="VF195_11630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581508.1"
                     /translation="MPIRVVLAEDQYIVREGIRRLLETQPDVEIAAVCDDLDSLLAAV
                     EAEKPDVVVTDIRMPPSNADEGIQVAERLRETLPDTGVVVLSQYATPSYALALLENGS
                     AGRSYLLKERVQDVEQLVSAIRTVAEGGSVIDPKVVEALVAENARGERSPIKQLTPRE
                     RDVLREMAAGKSNAAIGESLFLAERSVEKVIHSIFLKLGLTWETSVHKRVKAVILYLG
                     END"
     gene            complement(3051..3665)
                     /locus_tag="VF195_11635"
     CDS             complement(3051..3665)
                     /locus_tag="VF195_11635"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX6581509.1"
                     /translation="MRREIAELRASRERLALANDAERRSLEHALHEGVQQLLVGLAAN
                     LQIATRSIDADPATAKQLLAETGRDAQQALEETRRLANRLYPPLLEAGGLGVALRSAA
                     ANANVPVRIDVAADAAYPPEIAGAVYFCCLVFLEGVDASTPVAIRVGDEVGTLTFEIE
                     AEGDVDVGRLPIRDRVEALGGRLTIEGSGDRTRAVGSLPLPEGG"
     gene            3940..4437
                     /locus_tag="VF195_11640"
     CDS             3940..4437
                     /locus_tag="VF195_11640"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6581510.1"
                     /translation="MIRRTMMVAIVGLVALGVGLIGNALATTPSGFAAETARGPLVDR
                     PLDVNWHFGPESKVKLQTQGPVEFAAQRVVIQPGGTLGWHSHPGPTLVTIFSGTMSFY
                     HAEHCTERIEYTAGESFSNLPDEIHMARNEGSVALVLYASYFVPAGAPLIRIDQPSPG
                     PECPV"
     gene            4564..5190
                     /locus_tag="VF195_11645"
     CDS             4564..5190
                     /locus_tag="VF195_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581511.1"
                     /translation="MSAIARTKADPNVSVAQASLAALSMTAAAIHFAVMGEHFTEYAA
                     FGVFFSVVAWAQAVWAVGVIVLPSRRLLIVGLLGNALVILVWLSSRTTGLPIGPEAGA
                     PETAAFVDLLSTILEVAIVAGTAVLLLRGRPTHSMGGLPVWLGLAGLVVVLAGLTTWS
                     VAAGATSGGEHGIDHEDGHAAASAGLPGFARVHLGDGGWSVQVPVDAS"
     gene            5366..6352
                     /locus_tag="VF195_11650"
     CDS             5366..6352
                     /locus_tag="VF195_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6581512.1"
                     /translation="MPLTSTLPIEGRFPSLAGATEWLHSPSLDPAGLREKVVLIDFCT
                     YTCINWLRTLSYVRAWAGAYRDDGLVVIGVHTPEFSFEHDLDNVRRALASMNVDWPIA
                     VDNDYAVWDAFANHYWPALYFIDAEGSIRHHWFGEGDYERSKSVIQQLLRGAGAVDVG
                     DELVAIDPVGPEVEADWDNLGSSETYLGGDRSRNFASRDRMSIGQTQTHVAPESLRLN
                     DWALSGDWTVEADRVSSIEPNGRISFRFHARDVNLVIGPRVRDAPVPFRIMIDGDPPH
                     RGFGSDADEDGAGLLEEPRMYQLVRQREPIIDRLFEIEFLDRGAEAFAFTFG"
BASE COUNT         1049 a         2265 c         2175 g         1115 t
ORIGIN      
        1 ccgacgtcac gtcgacggcc ccacccacgg gcgtgaactt cacggcgttg gacaacaggt
       61 tgaagatcac ctgctggatg cgccgctcgt ccccctcgac gacgtcggca tccggatccg
      121 cgtcgagcgc aacgctgaca ccatcgtcgc tcgcccgctc ccgcacgacg acgacccccc
      181 tctcgagcgc ctcccgcagc gagaacggtg ccttctccag ttcgacctgc ccggcctcga
      241 ccttggacag gtccaacaca tcgttgatca gggcaagcag gtggttcgcg gaggagagga
      301 tgtcgtcgag gtattcagcc tgcttctcgt tcaccgcgcc ggccatcccc tcggacaaca
      361 cctgcgagaa cccgatgatc gcgttcagcg gcgtgcgcag ctcgtgcgac atgttggcca
      421 ggaaatccga cttgtgctgg ctcgccgcct cgagctccgc gtagaggcgt ttgagctcgt
      481 cgttcgtccg gttgacgttc gcggcgagcg cgccgagctc gtcccggttc tcgacgtcga
      541 cgtgccccga gaagtctccc gccgcgatcg tggccaggcg ggaatcgatc cgctggatgg
      601 gaccgaccac cgaccacgac aggacgaagc cgagcacgag cgccaggccg atcgccccca
      661 cggcgacccc gacgtagagg ctccgcgagc tctcgtacgc gcgtgcgttg tcggcgatga
      721 caacgttggc tttcgccgtc gtggcattgg cgagctccga agcgagctgg tcgaggccct
      781 cagccagcct ttccgcctcc gcgcgtacgg cctcgttggg atctcggatc acctcttcca
      841 tcagcacgga aaggcgatcg ctctcctcgc ggatctcacg gaggacgctc tcgtcctcgg
      901 ccggaggcac gaatcccagc cggtcgggga aggtcgtcgc tgggatccgc cggacctggt
      961 tcgcgatcgc ctggtcgacg gcgacgcgaa cgcccagggc tgagtcatcc ggccacacca
     1021 cgaagtagtc ggagttccgg ccgaggttct cggcgaggag gttgcgaacg ttcgaggtgt
     1081 cgctctggag cttgccgtac gcggcggctc gttcctggag aggcccgagc tcgctcaccc
     1141 ggtcgtccga ttgtgcgagg acgcgcagcc cgagggcacc gacggcgacg agcagcaccg
     1201 cggtgcccac gaaggcaatg agcagcttcg tgtggaccct ggcgggcagg cgacccacgg
     1261 tccggacgag tcgatagtcg cccgggctca tcggccggcg tttcacggcg acggcctccc
     1321 ctccccgttt cgccgaccgg gctgccgaca ccggctcaga gggtcagctc ggtcgatgcc
     1381 ccaaccatgt tggtgatcag ccaccgtccg tcgatccgcg cgacgtcctg atcggtgccc
     1441 gtgatcgcct cgatctcgcc ggtctggtag ttcacgaagt ggcactcgaa gtgcaacgtg
     1501 ccccggtccc cgttgaccgt gatctccatc ttgtacgccg ggtggtcggt gatccacgtc
     1561 gtctccggcg cgaaggccac cgactgctcg agccagaact gccggatctc gtccactccg
     1621 gccgcggtcg cctcgggacc cacggtcagg gtggcgttcg gtgcccacag gctcaccatc
     1681 aggtcgatgt ccttcgtcgt catggcctgg tggaagacct tctcgatctg ctcgatctca
     1741 tacttgtcgg ccttcagttg cagcgcggcg gtctcggtat cggagcccga gtccccgcag
     1801 gccgcgagcg aaacgaccat gactgcaagt acgccaacga tcaacagcct ccgcatgtgc
     1861 gctcccctcc atcgtgttcg cctgggcgat cggtgcggat gttcccaccg cgacacgtaa
     1921 tccgtctact gcgcgaccgt agaaccgggc ccggtccgtg cctaccgtgc tggcacggca
     1981 tcgcgagggc ggtccttcac ccggttgctt ctttgcagta ccgacgcacc tgatcggcga
     2041 acgtgtcgat gcggatcggc ttctcgatcc accccgcgaa gccggcagcc tcgagccagt
     2101 cgccgctcgc ctcgagccgc atggcgctca ccgcgaccac cgggatccga gccgtccgct
     2161 cttgctcccc gagcgtccgc atggcctcgg tgccgtcgat gtccggaaga cggaggtcca
     2221 tcaggatcac gtcgggaacg tgcttggctg cgagcgcgag cgcctccggc cccgtcgccg
     2281 cctcgatcgt tcggaagccg gcagcggaca ggacgcttcc ggcgagcttc cggttcttct
     2341 cgttgtcgtc gacgatcaag acgacgggca ccggcgtatc ctccggccat gccgatccgc
     2401 gtggtgctcg cggaggacca gtacatcgtc cgcgagggca tccgccgtct gctcgagacg
     2461 cagcccgacg tcgagatcgc ggcggtgtgc gacgatctcg actcgctgct cgccgcggtg
     2521 gaggcggaga agccggacgt cgtcgtcacc gacatccgaa tgcctcccag taacgccgat
     2581 gaggggatcc aggtggccga acggctgcgc gagacgcttc ccgacaccgg cgtcgtggtg
     2641 ctcagtcagt acgccacccc gagctacgcg ctcgccctcc tggaaaacgg cagcgcgggg
     2701 cgctcctatc tcctgaagga acgcgtccag gacgtcgagc aactcgtgtc ggcgatccgc
     2761 acggtcgcgg agggcgggtc cgtgatcgac cccaaggtcg tcgaagcgtt ggtcgccgag
     2821 aatgctcgcg gcgagaggtc gcccatcaag caactgaccc cgcgcgagcg cgacgtgctg
     2881 cgcgagatgg cggcaggcaa gagcaacgcg gcgatcggcg aatcgctgtt cctcgccgaa
     2941 cggtccgtcg agaaggtgat ccactcgatc ttcctcaagc tcgggctcac gtgggagacg
     3001 tcggtccaca agcgcgtcaa ggcagtgatc ctctacctgg gcgagaacga ctagcctcct
     3061 tccgggagcg gcagggagcc gaccgctcgg gtccggtcac ccgatccctc gatggtcagc
     3121 cggccgccga gagcctcgac ccgatcgcgg atcgggagcc gtcccacgtc cacgtcgccc
     3181 tccgcctcga tctcgaaggt gagtgtcccg acctcgtcgc cgacccggat cgccaccggc
     3241 gtgctggcat cgacgccctc gaggaagacg aggcagcaga agtaaaccgc gcccgcgatc
     3301 tcggggggat atgccgcgtc cgctgcgacg tcgatccgga ccggtacgtt cgcgttcgcc
     3361 gccgccgagc gcagcgccac tcccagacct cccgcctcca acagcggcgg atagagcctg
     3421 ttcgcgagcc tccgggtctc ttcgagcgcc tgctgcgcgt cgcgtccggt ctccgcgagg
     3481 agttgcttcg cagtcgcggg atcggcatcg atggatcgcg tcgcgatctg gaggttggcg
     3541 gcgaggccga ccagcagttg ctgcacaccc tcgtggaggg cgtgctcgag gctgcggcgt
     3601 tcggcgtcgt tcgccagggc gagtcgctcg cgcgacgcac gaagttccgc gatctcgcgg
     3661 cgcaacgcgt cgaactggcc atccgccgtg gcgctcaggg cgatcgacct cctcccctgt
     3721 caccggaagt tctccgtcat cgccgcggcg acgtcaagcc tccggcatcg tagtgggacg
     3781 gaaggcagca tcccccggac tcaccaggct gacaccgtag agccgcctcg agccacctcc
     3841 tagcgtcgct gcacacgacc aacgggcatc gcgcaagggg aagggaggga tcggcagaag
     3901 gggaaagtgc cgcggacgac gttgcaaagg aggagatcca tgatccggag aaccatgatg
     3961 gtggcgatcg tcggactcgt agcgttggga gtcgggctga tcggaaatgc cctggccacg
     4021 acccccagcg gatttgccgc agagacggcc cggggacctc tggtcgaccg gcccctcgac
     4081 gtgaactggc atttcggacc cgaaagcaag gtgaagctac agacgcaggg cccggtcgaa
     4141 ttcgcggctc agcgggtcgt catccagccg ggcggaacgc ttggttggca cagccacccg
     4201 ggcccgacgc tggtcacgat cttcagtgga accatgagct tctaccacgc cgagcactgc
     4261 accgagcgga tcgagtacac ggcgggagag tccttctcga acctgcccga cgagatccac
     4321 atggctcgga acgaaggttc ggttgctctg gtgctctatg cgtcctattt cgtgccggcg
     4381 ggggcgccgc tcattcggat cgaccagccg tctccgggcc ccgagtgtcc ggtgtaggag
     4441 tcacgcgaga gtggggcgcc gaccggcgcc ccactcggct cttcagcctt cttcccacgc
     4501 aggggtctcg gcgccgtagt cagggagggt ctgttgatgg atgttcgcga aaggagcatt
     4561 cccgtgagcg cgatcgcacg caccaaggcg gacccgaacg tctcggtagc tcaggcctcg
     4621 ctcgccgcgc tgtcgatgac agcggccgcc atccacttcg cggtcatggg agaacacttc
     4681 accgagtacg cggccttcgg ggtgttcttc tccgtcgtcg cctgggcgca ggccgtctgg
     4741 gcggttggcg tgatcgtcct tccatcgaga cgcctcctga tcgtggggct cctcgggaac
     4801 gccttggtga tcctggtgtg gctctcctct cggacgaccg gcctgccgat cggccccgag
     4861 gcgggcgctc ccgagaccgc tgcgttcgtc gacctcctct caacgatcct cgaggtggcg
     4921 atcgttgcgg ggacggccgt gctcctcctt cgcgggcgac caacgcattc gatgggcggc
     4981 ctcccggtct ggctcggcct cgccgggctc gtcgtcgtgc tggcggggct gaccacgtgg
     5041 tccgtggcgg ccggcgcgac ctccggaggc gagcatggca tcgatcacga agacggccac
     5101 gcggcagcga gcgcaggact gcccgggttc gcccgcgtgc acctcgggga cggcgggtgg
     5161 tccgtacagg tgcccgtcga cgcctcatag gccatcggga cttccccatc gggtacgacg
     5221 ctggagactc ggaccggacg ttcaacgtcg accgtcacac ctctgacgag cagagattcg
     5281 acaggcatac aatctgcccc aggaagcgcc cgccgcgttc gccgacgcga tcgtcgacgt
     5341 cgccacgaac tgagagcgag ctcagatgcc actgacctcc acgctcccga tcgagggacg
     5401 cttcccctcc ctggccggcg cgacggagtg gctccactcg ccctccctgg atccggccgg
     5461 cctgcgagaa aaggtcgtgc tcatcgattt ctgcacgtac acctgcatca actggctgcg
     5521 gacgctctcg tacgtccgag cgtgggcggg cgcgtaccgg gacgacggac tcgtcgtcat
     5581 cggcgtgcac acgcccgagt tctcgttcga gcacgacctc gacaacgttc ggcgggcgct
     5641 ggcctcgatg aacgtcgact ggccgatcgc ggtcgacaac gactacgcgg tctgggacgc
     5701 gttcgccaac cactactggc cggccctgta cttcatcgac gccgagggga gcatccggca
     5761 tcactggttc ggggagggcg actacgaacg ctccaagtcc gtcatccagc agctcctgcg
     5821 tggtgccgga gcggttgacg tcggggacga gctcgtcgcg atcgacccgg tcggacccga
     5881 ggtcgaggcg gattgggaca acctcggatc gtccgagacc tacctcggcg gcgaccgatc
     5941 gcggaacttc gcctcgcgag atcggatgtc catcggacag acacaaacgc atgtcgcccc
     6001 cgagagcctg cggttgaacg actgggcctt gtcgggcgat tggacagtcg aggccgatcg
     6061 cgtttcctcg atcgagccga acggccgcat ctccttccga ttccacgcgc gcgatgtcaa
     6121 tctcgtgatt ggaccgaggg tacgcgacgc gccggttccg ttccgtatca tgatcgacgg
     6181 cgatccgccg catagaggct tcggcagcga cgcggacgag gatggagccg gcctgctaga
     6241 ggagccgcgg atgtatcagt tggttcggca gcgggagccg attatcgacc gcctgttcga
     6301 gatcgagttc ctcgatcgcg gcgcagaggc gtttgcgttt acgttcgggt gaacacgtcg
     6361 gaacagaccg agtcgccaga cgtcgcccac cctttgtcca tgccgtcagc tgccacgggg
     6421 gtgcactcca agcatcgcct cggacgaggg tagcgcggcc actgatccgt ctctttcggt
     6481 tggccgcttc gaggtcaggg ctccagcagg ggttcgagcc actcgggcgg gacccgcccc
     6541 tgcaggcgca ggaacggggg ttcgaggtcg ggcacccgtt cgagggtggc gacggcttcc
     6601 ccca
//
LOCUS       DASUYD010000221         8430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1260946, whole genome shotgun sequence.
ACCESSION   DASUYD010000221 DASUYD010000000
VERSION     DASUYD010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260946"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..150
                     /locus_tag="VF195_11655"
     CDS             1..150
                     /locus_tag="VF195_11655"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX6581513.1"
                     /translation="MATVRDPVCGMEIDPATAVASEEYEATTYYFCSQSCHDSFTAQP
                     EKYAA"
     gene            1270..1518
                     /locus_tag="VF195_11660"
                     /pseudo
     CDS             1270..1518
                     /locus_tag="VF195_11660"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1858..3699)
                     /locus_tag="VF195_11665"
     CDS             complement(1858..3699)
                     /locus_tag="VF195_11665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6581514.1"
                     /translation="MEGRAIAGFRILRELGRGGMGVVYLARQDYPERDVALKVVSPEF
                     AGDPAFRDRFIAEANAVAGTEHPNIVPVYAAGVEDDTLYLVMRYVRGSDLASEVARGD
                     LPPERAVEICTQIGRALSSAHQRGLVHRDVKPGNVLLDEEGHAYLMDFGLIRRTDVRG
                     GPTRTGQFMGSVAYCAPEQIRGDTIDGRADLYSLGCVLFECLTGRPPFYRENEVAVMY
                     AHLEELTPRPSDHAPGVPRGLDDVVANAMAKRPEDRFRTAEEMVAAMRTAMSTGARPR
                     SRARPLAIAIASIAVAIVVGALAIALSRSNGSEEAVSPPTGGTSPSGGNEFQQGLLVT
                     DPETGEEIARYPGSFGTGVFASGHAEVIAAGNGAIWTTDDQVRVLRIEPSTGEATPVP
                     RADCGLDAIATGLGSTWVVCPGSTVGTVVQIDPFELTGRDIKMPGNLGATSDVGTGGA
                     NMWIAASGPLVRFNQLDRRAEVVEGLIADDVAVDRNDVWIVDELASTVSRVDPKTGAP
                     VQTIEVSGAIDDVAAGLGSVWVLDGGAGTVTRIDPGTGEAADPIGVGPGASTIRLGFG
                     AAWVCVPAERRLVRIDPVSGVPEPFDVGAPARVAVADPVTDSLWLLV"
     gene            complement(3979..4482)
                     /locus_tag="VF195_11670"
     CDS             complement(3979..4482)
                     /locus_tag="VF195_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581515.1"
                     /translation="MASGPGMIDRRLHQRLSHARPCNILGHVEAAEISCPRGDVRIGA
                     RQHVCVADDPTGPLRDEDGGARGKLGQPFGEVGGGFLHRQGFQVRLREEIAIAPAPRS
                     NAHLRDGSGIARLCLTNDELASHERSVARGMYWARGSRVPPDPVLEDRRLPGKACRSP
                     YLRFCSR"
     gene            4997..7081
                     /locus_tag="VF195_11675"
     CDS             4997..7081
                     /locus_tag="VF195_11675"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX6581516.1"
                     /translation="MEQRDHDMQPSADAGATTHRRRRPTGAAPPLPKQIGSTGWVWLI
                     LLVAVVVTGCWWLRVDPGPLDRFDAWITDALISLRVGWLDTLARQAHTIGSRVGFAAL
                     GVLLVLATVWFRRWRHLLIWMISLGVAGALLQGLELVSLRPRPFGVQQLASWEGYATP
                     SIPIGAVAIISIGAAFMLVVPGRPRFWAKLAAAGAIAIIGMLRIYLGVDHFTDVVSGA
                     IVGVAIPLAAFRLFAPNDLFPVSYGAHGKAAHLDVSGRRGEAIRTASQEQLALTVHDI
                     KPVGLEGSGGSTPLKLTVTDEDGRERTIFAKLYAKSHVRADRWYKLGRTMLYGRLEDE
                     TPFTTVRRFVEYEDYTLRMLGEYGFPTPAPLGIVEITPEREYLIAMDFFDDAVEIGEA
                     QIDAHVIDQGLAMIRLMWDVGLAHRDIKPANLMVQRRELKLIDVFFVQVRPSPWRQAV
                     DLGNMMLVLALRSDARTVYDAALRYFTPDELAEAFAATRGVASPTQLRQQMKQDGRDL
                     LEEFRSMAPSRRSIAVQRWSVRRVALIVASLLVLLLAGLTGVGLLFPTRGTVTTPTCG
                     TGRSMQLMAQAVPSATRLPCVASLPVGWSVGTAETIRGKANFAVGVGDGSSEPVTVTL
                     TGSCPAPVEGTQQIPIEGGCVTYTPTITGRDVPSFAPDRGLSFTSRADLVAAVESHYD
                     QVLCGALATPCQ"
     gene            7410..7772
                     /locus_tag="VF195_11680"
     CDS             7410..7772
                     /locus_tag="VF195_11680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX6581517.1"
                     /translation="MLKAHRKAQLEERAEAGLGRPRADGLIFTDEQGGPLHPNVVSRT
                     FGKLVREAGLPTIRLHDLRHTHSTLALQAGVHVKIVSERLGHSSVTITLDTYSHAIPG
                     LQRDAAERVAALIPGLQR"
BASE COUNT         1376 a         2798 c         2837 g         1419 t
ORIGIN      
        1 atggcgacgg taagagatcc ggtctgtggc atggagatcg atccggcaac ggccgtggcg
       61 agtgaggagt atgaggcaac gacctactac ttctgttcgc agagttgcca cgacagcttc
      121 actgcgcagc ccgagaagta cgctgcctga cgaccggtcg agcgggcccg ggggcgatca
      181 ttcgtgtctt aagtgatgtc gcgaaatgtc atccttctga gcggatggcc gacaggagct
      241 gcttggtctt acgaacatcg actggtagtc ggcgcgccac gcgatgtgcc cagggctgga
      301 cacgctcgcc tccgcgtcga tcaagtagga gatccgcggt ccaaaccggt cgagctcggt
      361 gcgaatctga tcagcgggat tcaccactat tggcagcggc gtcaacccca gccgacaact
      421 tcttcgcgtg ctcttcgggt tctcgcatct gacagaagca cgtgtcgcag cccaccccct
      481 cgctcgttct tcacgcagcg gccatataca acggaacggg aacacgatgc agcacggact
      541 aaacacggtg accgtaccgg cgaagaaggc tgccaggatc gaggatccac ccgacaggag
      601 cgtgcccatc caggcggtcc tctccggcat ccgcaggagc ttcttgctcg ggagccgacc
      661 cgaactgaac aggcgaccgt cgatcacgac ggcaggtgag agggccggac gatggtgacc
      721 gacgatcgct cggcctccct ccgagtcgat ctcgaccacg ctcacatcca gcgggaacgt
      781 cgcgccgatc tcctcgagcg cctcgatggc gtcatcgcag aggcgacagg cgaggggcga
      841 acgacgacca cgccgattga acgggtctcc ttcgagggtg tgatggcgtt gcgaacgaag
      901 gagcgtctgc gggggtgtca agacgttgtg tatctgccgc gcacacagag tgaggtatca
      961 gacgcggccc gccggtggtc ccgtgggaag ggtgacgaag gtccctaacc aggcggccgc
     1021 agggttctac gccggcctca tccgtttcat ctcgacactc cagcgcatcg agggtcgacc
     1081 acagcaggtt gatcccaaga ctgaccgaag taggcgcact gtcgcactgc ccgaattcgc
     1141 tgtgggggcg ctcagagctc atcggaagga ccagaccgcc cgccggctgc tcgctggacc
     1201 tgcgtgggcg gatcttgatc tcgtcattga tcgcggcgac ggcaggccgg tggacccagg
     1261 gcacctttcg caaccttcgg ccggattgct cgtcgtgcgg ggctcccggg tattcggctc
     1321 cacgatcttc gccacgccta cgcaacggcg ctcctggccg cgggggtgca tccgaagatc
     1381 gcgagcgagg cacttgggca cgcctcggtg gggttcacga tggacgcgta ctcgcatgtg
     1441 ttgccgacga tgcaagagca ggcggctcgc gcgattcagg ctgcaatcgg cgctgccggc
     1501 ggggaatcta ctagctgact gttgccaaag tgttgccacg agtcaccccg acccgacggt
     1561 ccgactcgga tctctagagg gattccccag gtcagagggg gtgcgagagg ggagagtcga
     1621 actcccacgc cctttcgggc accggatcct aaggttgctg cggccaggca cggatgccgg
     1681 cgctacgtgt cgtcccgtgt cgtctggtgt cgtcctgtgc cgtccggtct cctcccgtcg
     1741 tgagcaagac gtgggcaatg accctatggc gaaggaaggt tgatatcacg gcgtcccaaa
     1801 cgcctcctgt ccttgaacca cgaaccactg cggtggcatc ctgccggtct cgcggagtca
     1861 aacgagtagc cagagcgagt cggtcactgg atcggccacg gccacgcggg ccggtgcccc
     1921 gacgtcgaat ggctccggaa ccccggagac agggtcgatg cggaccaagc gacgctcagc
     1981 tgggacgcac acccacgcgg cgccgaaacc gaggcggatc gtcgaggcgc cgggccctac
     2041 cccgatcggg tcggccgctt caccggtccc tggatcgatc ctcgtaacgg ttccggcgcc
     2101 cccgtcgagc acccagaccg atccgagtcc tgcagcgacg tcgtctatcg cgccggagac
     2161 ttcgatcgtc tgaacgggtg ccccggtctt ggggtcgacg cgggagacgg ttgaggcgag
     2221 ttcgtcgacg atccacacgt cgttccgatc gacggcgacg tcgtccgcga tgaggccttc
     2281 gaccacttca gcacgccgat ccaactgatt gaaccgcacc agtgggccgc tggcggcgat
     2341 ccacatgttc gcgccgccgg tcccgacgtc gctggtcgcc ccgaggttgc ctggcatctt
     2401 gatgtcgcgg cccgtcagct cgaacggatc gatctgcacc accgtgccca cggtgcttcc
     2461 cgggcacacg acccaggtgg atcccagccc tgtcgcgatc gcatcgaggc cgcaatcggc
     2521 tcggggaacc ggtgtcgcct cgcccgtcga cggctcgatc cggaggaccc tgacctggtc
     2581 atcggtcgtc cagatggccc cgttccccgc ggcgatcacc tccgcatggc cgctcgcgaa
     2641 cacgcccgtt ccgaacgacc ccggataccg ggcgatctcc tcgccggtct ctggatcggt
     2701 caccagcagt ccctgctgga actcgtttcc gccggagggt gacgtgccgc cggtcggcgg
     2761 gctcaccgcc tcctcggaac cgttggaacg cgagagggcg attgccaggg cgcccacgac
     2821 gatcgccacc gcgatggagg cgatcgcgat cgcgagggga cgagcccgcg acctcggccg
     2881 cgcgcccgtc gacatcgccg tccgcatcgc cgcaaccatc tcctcggccg tccggaaccg
     2941 gtcctctggg cgcttggcca tcgcgttggc gaccacgtcg tcgagtccgc gagggacccc
     3001 gggggcgtgg tcggacggtc gcggtgtgag ttcctccaga tgcgcgtaca tcaccgcgac
     3061 ctcgttctcg cggtagaacg gtggacgacc cgtgagacac tcgaagagaa cacatccgag
     3121 tgagtacagg tccgcacgtc cgtcgatcgt gtctccgcgg atctgctcgg gtgcgcagta
     3181 cgcgacggag cccatgaact gcccggttcg cgtcggacca cctcgcacgt cggtccgccg
     3241 gatcaacccg aagtccatga gatacgcgtg tccctcttcg tccaggagga cgttgcccgg
     3301 tttgacgtcg cgatggacga gaccacgctg gtgcgcggac gagagcgccc gcccgatctg
     3361 cgtgcagatc tccaccgctc gctccggggg gaggtcgcct cgcgcgacct ccgaggcaag
     3421 atcggaccca cgcacgtacc gcatcacgag gtacaaggtg tcgtcctcga ccccggccgc
     3481 gtagacgggc acgatgttcg ggtgttcggt accggccacc gcgttggcct cggcgatgaa
     3541 ccggtctcgg aaagctgggt cgccggcgaa ctccggcgag acgaccttga gtgcgacatc
     3601 gcgctcgggg taatcctgcc gggcgaggta gacgaccccc atcccacccc gcccgagctc
     3661 gcgcaggatg cggaagcccg cgatcgctct cccctccacg acggagtcct accacgcacg
     3721 acgggccgcg tcccatccgg ctacatagag ctcggcagaa gtcgcctggc agaaggtcgc
     3781 tcgcagttgc gagtgcgaga ggggggagtc gaacccccat gccctttcgg gcaccggatc
     3841 ctaaggttgc tgcagccagg cacagatccc ggctctacgt gtcgtcctgt gtcgtctagt
     3901 gtcgtcctgt gtcacccggt gtcgtcccgt cgtgagcagg atgtgagcaa gagccgatga
     3961 cccgaacgct ctctacgtct acctggagca gaaccgcaga tagggagacc ggcaggcctt
     4021 cccagggaga cggcggtcct cgagcacggg atccggcgga acgcgactgc cacgcgccca
     4081 gtacatccct cgggctacgc tgcgctcgtg gctggcgagc tcgtcgttcg tgaggcacaa
     4141 ccgggcgatg cccgagccat cgcggaggtg agcgttgctt cgcggcgctg gagctatcgc
     4201 gatctcttca cggaggcgaa cctggaagcc ctgtcggtgg aggaaaccac cgccgacttc
     4261 gccgaagggc tggccgagct tccccctggc gccgccgtct tcgtcgcgga gcgggccggt
     4321 cgggtcgtcg gctacgcata cgtgctgccg agccccgatg cggacgtccc cgcggggaca
     4381 gctgatctcg gcagcctcta cgtgaccgag gatgttgcag ggacgggcgt ggctcaagcg
     4441 ttgatggagg cggcggtcga tcatgcccgg gccgctggcc acgagctcct cacgatctgg
     4501 tacgccgaga gaacggccgc gcccgtcgct tctacgagaa gcagggtctg cagctggatg
     4561 gtggcaagcg ctcacgtccg cacgacgtcc tcccgttcga gatgcacgag attcgctccg
     4621 gatgctgctc gaaccgcgca ggccccagcc aggggactca gaagctacat ccagaacgtg
     4681 aagagcccca acaccgcaag gagtcggtgc tggggctctt cggtgacccg cgcgcaaagg
     4741 tgtgtggcgg cgacggaacg cgcgggccgg gtgacctccg cgacgctcgt tcaggtccta
     4801 cagtttgagc gccgcgcagg ccatcaggaa gaactggtgc gcggtccgca tcccgggaag
     4861 gagcgtcgcc cgggtatgag ctgtgcgggt gcacgtcgac gggctcggaa accggcgtgc
     4921 cgttgacggt cgcgccggtc atggtcggca tgcgtggcgt ccggccgatc gtgcgaggat
     4981 cttcgcggag ggaagcgtgg aacagcgtga ccacgacatg cagccatcgg ccgatgccgg
     5041 ggccaccact caccggcgtc gtcggccgac gggcgccgcc ccgccgctgc ccaagcagat
     5101 cggttccacg ggttgggtct ggctgatcct gctggtggcc gtcgtcgtca ccggctgctg
     5161 gtggctccgg gtcgatccgg gcccgctcga ccggttcgac gcatggatca ccgatgccct
     5221 gatctcgctt cgggtcggct ggctcgacac gctcgcgcgc caggcgcaca ccatcggttc
     5281 ccgcgtgggg ttcgcggcgc tcggtgtgct cctcgtgctc gcgacggtgt ggttccgccg
     5341 ttggcgccat ctcctgatct ggatgatctc cctcggcgtc gccggagctc ttctgcaggg
     5401 gctcgagctc gtctccctgc ggccgcggcc gttcggcgtg cagcagctcg cgagctggga
     5461 gggctacgcc acgccctcga tcccaatcgg agccgtcgcg atcatctcga tcggggcggc
     5521 gttcatgctc gtcgttcccg ggaggccgcg attctgggcg aagctcgccg cggcgggggc
     5581 gatcgcgatc atcggcatgc tgcggatcta cctcggggtc gaccacttca ctgacgtcgt
     5641 gtccggtgcg atcgtagggg ttgcgatccc gctggccgcg ttccggctct tcgctcctaa
     5701 cgacctcttc ccggtcagtt acggtgcgca cggcaaggcg gcgcacctgg acgtcagcgg
     5761 gcgtcggggc gaagcgatcc gcaccgcgtc gcaggaacag ctcgcgctca ccgtgcacga
     5821 catcaagccg gtcgggctag agggctccgg cggctccacc ccactgaagc tcacggtgac
     5881 tgacgaggac ggccgcgagc ggacgatctt cgcgaagctc tacgcgaaga gccacgtgcg
     5941 cgcggaccgg tggtacaagc tcggccgcac gatgctctac ggacggctcg aggacgaaac
     6001 gccgttcacg acggtccgcc ggttcgtgga gtacgaggac tacaccctcc gcatgctcgg
     6061 cgagtacggg ttcccgacgc ccgcgccgct cggcatcgtg gagatcaccc cggagcgcga
     6121 gtacctgatc gcgatggact tcttcgacga cgcggtcgag atcggtgagg cccagatcga
     6181 cgcgcacgta atcgaccagg ggctcgcgat gatccggctg atgtgggacg tggggctcgc
     6241 ccaccgggac atcaagccgg ccaacctgat ggtgcagcgc cgggagctga agctaatcga
     6301 cgtcttcttc gtccaggtcc ggccctcccc gtggcgtcag gccgtcgacc tcgggaacat
     6361 gatgctggtg ctggcgctgc ggtcggacgc gcggaccgtc tacgacgccg ccctccggta
     6421 cttcacaccc gacgagctgg ccgaggcgtt cgccgcgacg cggggcgtcg cgagcccgac
     6481 gcagctgcgc cagcagatga agcaggatgg ccgcgacctg ctcgaagaat tccggtcgat
     6541 ggcgccgtcg cgccgttcga tcgcggtgca gcgctggagc gttcgccggg tcgcactgat
     6601 cgtcgcctcg ctcctggtgc tgctgctcgc tggcctgacg ggagtcgggc ttctcttccc
     6661 cacgcgcggc accgtgacga ctcccacgtg cggcaccggg cggtcgatgc agctgatggc
     6721 gcaggcggtg ccgagcgcca cgcggctccc gtgcgtcgcg agcttgccgg ttggatggag
     6781 cgtcggcacc gctgagacga tccgaggcaa ggcgaacttc gcggtcggtg tcggcgacgg
     6841 ctcgagcgag cccgtgacgg tgacgctcac cgggtcctgc ccggcgccgg tcgaggggac
     6901 ccagcagatc ccgatcgagg gcgggtgcgt gacctacacc ccgaccatca ccggccggga
     6961 tgtaccgtcg ttcgcgccag accgtggtct ctcgttcacc tctcgcgccg acctcgtggc
     7021 ggccgtcgag agtcattacg accaggtgct gtgcggcgcg ctcgccactc catgccagta
     7081 gggacgagcg atcgactctg ccctctcaga cgactgaact ggatgcggcc cgtcttgggt
     7141 ctgcggtcaa tcggttgtga tccccgagct cgatcccgta tctctcgtgc gagctagcac
     7201 acgcaggaag gcctcttggg cactacccgc acacgcacga gaactatccc cgcgtcagaa
     7261 gaggtgcgag aggggagagt cgaactccca cgccctttcg ggcaccggat cctaaggttg
     7321 ctgcggccag gcgcggatct cccctctacg tgtcgtccta tgccgtctgg tgtcgtcctg
     7381 tgtcacccgg tgtcgtcccg tcgtgagcaa tgctgaaggc gcatcgcaag gcgcagctcg
     7441 aggagcgcgc cgaggccggt ctcggtcgtc cccgtgccga cggcctcatc ttcaccgacg
     7501 agcaaggtgg cccgcttcat ccgaacgtgg tgtcgaggac attcgggaag ctggtgcggg
     7561 aggccggact tccgacgatc cgattgcatg accttcgtca tacccattcg acgctggcgc
     7621 tgcaggcggg ggtccacgtg aagatcgtgt cggagcggct cgggcacagc tcggtcacga
     7681 tcacgctcga cacctactca cacgcgatcc cgggacttca gcgcgatgcg gccgagcgcg
     7741 tggcggctct gatccccggt ctccagcgat agcgcctcca aggcacctct catatgctcg
     7801 ccgcgtggtc gggaaggggt tctccccgcg cgagtggcgg gtcgccaaaa ggaaggtcag
     7861 gacgttcctc gtcaaggtcg cccgaggaca gagcaccgtc ttctacaagt acgtcgcttc
     7921 gttgctcaac gacactcgaa tcaagcccca gtcgcgggcc atgcgggagg tattgacgga
     7981 gatctccagg ccgagcacgc cgcggggagg cccttgttga gcgccgtcgt cgtgaggacg
     8041 gatactcgcc gtccgggcaa gggcttcgaa gcaatggcga aacttctgag agtgcgcggt
     8101 cgatcgagcc cgccgacgtt ctggaagatg cagcgcgacc gcgtctggaa gcacgtttgg
     8161 acgtagcgtt agccacgctt ccgtgagtaa gcgtgagcaa gaaggcctcc ctggccgatt
     8221 gccggtagag cgaggaggag aagattcgca ggtcagaagg ggtgcgagag gggagagtcg
     8281 aactcccacg ccctttcggg caccggatcc taaggctgct gcagccaggc acagatcccg
     8341 gctctacgtg tcgtcctgtg tcgtctagtg tcgtcctgta ccatccggtg tcgttccgtc
     8401 gtgagcagga tgtgagcgag aacggcgccg
//
LOCUS       DASUYD010000222         6463 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_381518, whole genome shotgun sequence.
ACCESSION   DASUYD010000222 DASUYD010000000
VERSION     DASUYD010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381518"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VF195_11685"
     CDS             <1..478
                     /locus_tag="VF195_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX6581518.1"
                     /translation="GLLTAFGRRGLLGGPARAIGLELPFTTAGAAVAAAFVSFPFVVL
                     AVEAGLRSLDTRLEDAAASLGGSRWYVLRRVTLPMLAPQLVAGTVLAWARALGEFGAT
                     ITFAGNFRGETQTMPLAVFEALQSDPGAATLLSLVLVVASLGILIALRGRFIGAPT"
     gene            475..1569
                     /locus_tag="VF195_11690"
     CDS             475..1569
                     /locus_tag="VF195_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581519.1"
                     /translation="MSLRATVAADRGPFEVRAAIAAQEGETLALLGPNGAGKTTVVLA
                     LAGLIDSTGAIELGAERIDGLPPERRPLGVTFQNGALFPHMSALANVAFPLRARGIRR
                     RPAQEKASAILARLAPDVRPEALPSTLSGGEAQRVALARAIVHEPRLLLLDEPSSGLD
                     VRGRAELRPVLVDALASFPGVRVLVTHDPVEAMTLADRVAILERGRLTQEGTFDDVRR
                     TPRSRYAAELVGVNLFVGRLEPIDGGAGRLVTPEGDVVVGWPEGTLRRPIDGVTGTLR
                     PSDVVVHADLPTRSSARNVWRGTVEDLAIYGERVRARIGSRPPLVAEVTAGSVHQLGL
                     ARGSEVWASCKAVEVEISLPASEGGRTYPE"
     gene            1570..2226
                     /gene="lepB"
                     /locus_tag="VF195_11695"
     CDS             1570..2226
                     /gene="lepB"
                     /locus_tag="VF195_11695"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX6581520.1"
                     /translation="MKTDQRSLRKTDGDGESPRPKKRRHPALTFLGELPGLILMAFAL
                     ALLIKSTLLQAFWIPTGSMEPTLVPGDRVIVAKVPYYFHEPQRGDVIVFEEPDPAKEP
                     ERGVWGAITHWLGQGLGFSPPDNSDYIKRVIGEPGDVVSARNGDVYVNDVRISEPYLH
                     ERTARFPETTVPTGELFVMGDNRSNSLDSRFGLGFVPVDRVVGKAVWIIWPVDNMAGF
                     "
     gene            2240..2629
                     /locus_tag="VF195_11700"
     CDS             2240..2629
                     /locus_tag="VF195_11700"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX6581521.1"
                     /translation="MRGLLIAGGLLVGLWLLAAVVLLVIGRKTIARELVGFLPNLIRL
                     FRGLLGDERVPRSSKVLLLLGAFWLASPIDLIPEFLPGIGALDDAIVAGLVLRHVVKR
                     AGPDVVKDHWRGDPKTIGLMLRAARVS"
     gene            complement(2701..4071)
                     /locus_tag="VF195_11705"
     CDS             complement(2701..4071)
                     /locus_tag="VF195_11705"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014143870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEX6581522.1"
                     /translation="MTTLEVDPLAKELGDRFQTAGHELYLVGGAVRDLVLGRLTPGVD
                     ADFATDATPAETSRLLRGWADALYLVGAKYGTVGARRHGNVLEITTFREERYPEDERK
                     PAVTFAKDIQTDLSRRDFTINAMAVRLPDGEFVDPYEGVKDLAASVLDTPLDPEVAFS
                     DDPLRMLRAARFVSQLGVTPAPRVVDAIRRMRERLRIVSSERIRAEIDKLLVGDAAGA
                     GLTILVDSGLADEFLPELPALRLEQDPVHRHKDVLRHTFAVVERLEPDPVLRLAGLLH
                     DIGKPSTRAITPDGVSFHHHEVVGARMAEQRLRELRYPNAAVEEVRTLVELHLRFHGY
                     GDGWTDAAVRRYVRDAGPLLDKLNQLTRADCTTRDPKRAERFARLQDELEDRIARLAE
                     QENLDAIRPTLDGRQVMERLGVEPGPVVGEALAFLMDVRMERGEIPEDEAYELLDAWA
                     KEREIG"
     gene            4126..4491
                     /locus_tag="VF195_11710"
     CDS             4126..4491
                     /locus_tag="VF195_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581523.1"
                     /translation="MPVWLGFLWTLPNTLLGLVLGAFTLQIPRLDHGVVIFDHAARGL
                     TALLPRLGRTAMTVGVVIVSARPLEGRLLAHERQHVRQYLEWGPFFIPAYLVVAVRYG
                     YRHHPMELAAVRAAEAAEP"
     gene            complement(4496..5614)
                     /locus_tag="VF195_11715"
     CDS             complement(4496..5614)
                     /locus_tag="VF195_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX6581524.1"
                     /translation="MDIDELLRYTVERGASDLHLKAGNVPFVRVDGELSPAELGYLSA
                     ADTDRFAHALMSEHKKREFEQTNEADLGYTLSGVGRFRVNVFRQRGVIGLAIRRVRAE
                     VPSFEELRLPPVMSTLAASPRGLILITGPTGTGKTTTIAAMIGHINRTRRAHIVTVED
                     PIEVVHDDQLSIVQQREIGIDTESYAAALRHVIRQDPDVIFIGEIRDADSALAAIQAA
                     ETGHLVISTLHTIDAMETINRTLDLFPPEQQREIRSSFAGALRGIVSQRLVSRADGKG
                     RVPVVEVLVNTGRVFDRIVDPDETSGILEVIQGGEFYGMQTFDQALVKLVKEGVVSPD
                     EARRTATNPHDFDLALHGTLERRAAFDEATAASESLPY"
     gene            5727..>6463
                     /locus_tag="VF195_11720"
     CDS             5727..>6463
                     /locus_tag="VF195_11720"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX6581525.1"
                     /translation="MAEGGGASTPSADAGGSKRRRRRRPRRGTAHAAGDAKAQGAIRG
                     GDAAGDAPRSSARTDRAGAAAGRIHAETGQGQKRKAQRKSPRKAQKKQPKKEQKSQKQ
                     RQKRGGTSGKPSRRTRGAPTKREISAGGVVYRRGGDEIEIVLASRRTRRGELAWGLAK
                     GGIEDNESPEEAAVREVREETGLFAEIEDSLGETRYFYVWEDVRIRKTVHFFLMRHTG
                     GNIDDRDDEMEEIRWFPLERALKRAAYR"
BASE COUNT          945 a         2223 c         2265 g         1030 t
ORIGIN      
        1 cgggctgctc acggcgttcg ggcgccgagg actcctggga ggaccggcgc gcgcgatcgg
       61 cctggagctc ccgttcacca cggcgggggc cgcggtggcg gccgcgttcg tgtcgttccc
      121 gttcgtcgtg ctcgccgtcg aagccgggct ccgctcgctc gacacccgac tcgaggacgc
      181 cgccgcctcc ctcggtggat cgaggtggta cgtccttcgg cgggtcaccc tcccgatgct
      241 ggctccgcag ctggtggcgg gaaccgtgct ggcctgggcg cgcgcgctcg gggagttcgg
      301 cgcgacgatc acgttcgcgg gcaatttccg cggggagacc cagacgatgc cgctcgccgt
      361 gttcgaggcg ctccagtcgg atcccggtgc cgcgaccctc ctctctctgg tcctggtcgt
      421 cgcgtcgctc ggcatcctga tcgcgctgcg aggtcggttc atcggggcgc cgacgtgagc
      481 ctgcgagcaa cggtcgcggc agaccgaggg ccgttcgagg tccgcgcggc gatcgcggcc
      541 caggaaggcg agacgctcgc gctgctcgga ccgaacggtg cggggaagac cacggtcgtc
      601 ctggcgctcg ccgggctgat cgatagcacc ggtgcgatcg aactcggagc cgaacggatc
      661 gacggccttc ccccggagcg acggcccctc ggcgttacct tccagaacgg cgccctcttc
      721 cctcacatga gcgctctcgc gaacgtggcg ttcccactcc gggcccgtgg catccggcgc
      781 cgccccgcgc aggagaaggc cagtgccatc ctcgctcgcc tcgcgcccga cgttcgaccc
      841 gaggccctgc catcgacgct ttccgggggc gaggcgcagc gcgtggcgct cgctcgggcc
      901 atcgtccacg agccacgact cctcctgctc gacgaaccct cctcgggtct cgacgtccga
      961 gggcgagcgg agctccgccc cgtgctcgtc gacgcactgg cgtcgttccc cggggtccgc
     1021 gtgctcgtga cccacgaccc tgtggaggcg atgaccctcg ccgatcgcgt ggcgatcctc
     1081 gaacgcgggc ggctcaccca ggaggggacg ttcgacgacg tgcgccggac cccgcgaagt
     1141 cgatacgcag ccgagctggt cggtgtcaat ctcttcgtcg ggcggctgga gccgatcgac
     1201 ggcggggcgg ggcgcctcgt cacccccgag ggcgacgtgg tcgtcggctg gccggaaggg
     1261 acccttcgcc ggccgatcga cggggtgacc gggaccttgc gaccgtcgga cgttgtcgtg
     1321 catgccgacc tgccgacgag gagctccgcc cgcaacgtct ggcgcggaac ggtggaggat
     1381 ctcgcgatct acggggagcg cgtccgagcg cggatagggt cgcggccccc gctcgttgcc
     1441 gaggtcacgg cgggctccgt ccaccagctc ggcctggccc ggggctccga ggtctgggcc
     1501 tcctgcaagg cggtcgaggt ggagatctcg ctgcccgctt ccgagggtgg gaggacgtac
     1561 cctgagtgag tgaagacgga tcagcgctcc ctccggaaga cagacggaga cggcgagagc
     1621 ccacggccca agaagcgccg ccacccggcg ctcacgttcc tcggcgagct gccgggcctg
     1681 atcctcatgg cgttcgcctt ggcgttgctc atcaagagca ccctccttca ggcgttctgg
     1741 atccccaccg gttcgatgga acccaccttg gtcccgggcg accgggtcat cgtcgccaag
     1801 gtcccctact acttccacga gccgcagcgc ggtgatgtca tcgtgttcga agagcccgat
     1861 cccgccaagg agccggagcg cggcgtgtgg ggtgcgatca cccactggct cgggcaaggc
     1921 ctcggattct ccccgcccga caactccgac tacatcaagc gggtgatcgg cgaaccgggt
     1981 gacgtggtct ccgcgaggaa cggggatgtg tacgtgaacg atgtccggat ctcggagccg
     2041 tatctccatg aacgcacggc ccggttcccg gagacgacgg ttcccacggg ggagctgttc
     2101 gtgatgggcg acaatcgttc caattcgctc gactcccggt tcggcctcgg gttcgtgccg
     2161 gtcgaccggg tggttggcaa agcggtctgg atcatctggc ccgtcgacaa catggccggc
     2221 ttctagcgag ggcgatctcg tgcgcgggct gctgatcgcc ggtggcctcc tggtgggcct
     2281 gtggctgctc gccgccgtcg tgctcctggt gatcgggcgg aagacgatcg ctcgtgagct
     2341 cgtgggcttc ctcccgaacc tcatccgcct cttccgcggg ctgctgggtg acgaacgggt
     2401 gccgcgctcc tcgaaggtcc tcctgctgct cggagccttc tggctcgcgt ccccgatcga
     2461 cctgatcccc gagttcctgc cggggatcgg tgcactcgac gacgcgatcg tcgcggggct
     2521 cgtcctgcga cacgtcgtga agcgggccgg ccccgacgtc gtgaaggacc attggcgggg
     2581 agaccccaag accatcggac tcatgcttcg cgccgctcgc gtctcctaga cgcaccagga
     2641 acccaggcgt cgaacgaggg acgaggagtt cctcgatcgg cttcggtccg cttccgggcg
     2701 ctacccgatc tcccgctcct tcgcccaggc gtccaacagc tcgtacgcct cgtcctcggg
     2761 gatctcgccg cgctccatgc gcacgtccat caggaacgcg agagcctcgc ccaccacggg
     2821 cccgggctcc acgccgagtc gttccatcac ctgccgaccg tcgagggtcg ggcgtatggc
     2881 gtcgaggttc tcttgctcgg ccaggcgggc gatccggtcc tcgagctcgt cctggagccg
     2941 ggcgaaccgc tccgcccgtt tcgggtcgcg tgtcgtgcaa tccgctcgcg tgagctggtt
     3001 caacttgtcg aggagcggtc ccgcgtcgcg gacgtaccga cggaccgcgg catcggtcca
     3061 gccgtcgccg tacccgtgga agcggagatg gagctccacg agcgtccgga cctcctccac
     3121 cgcggcgttg ggataacgga gttccctgag tcgctgctcc gccatccgcg cgccgacgac
     3181 ctcgtggtgg tggaacgaca ccccgtcggg cgtgatcgcc cgggtcgagg gcttcccgat
     3241 gtcgtggagg aggccggcga gccggagcac gggatcgggc tcgagccgtt cgacgaccgc
     3301 gaacgtgtgg cgcaacacgt ctttgtgccg atgcaccgga tcctgctcga gccggagagc
     3361 cggcagctcg ggaaggaact cgtccgcgag ccccgagtcg acgagtatcg tcaacccggc
     3421 gcccgcggcg tcgccgacca ggagtttgtc gatctcggcg cggatgcgct ccgacgacac
     3481 gatccgcagc cgctcccgca tccggcggat cgcgtccacc acgcgcggtg ccggtgtcac
     3541 gccgagctgg gagacgaacc gcgcggcacg gagcatgcgc agcggatcgt ccgagaaggc
     3601 gacctccgga tccagcggcg tgtcgagcac actcgcggcg aggtccttca ccccttcgta
     3661 ggggtcgacg aactccccgt ccggcaaccg gacggccatc gcgttgatcg tgaaatcgcg
     3721 ccgggacagg tcggtctgga tgtccttcgc gaacgtgacc gcgggcttcc gctcatcctc
     3781 cggatagcgc tcttctctga acgtcgtgat ctccaggacg ttgccgtgcc ggcgcgcccc
     3841 gacggtgccg tacttggctc cgaccaggta cagcgcgtcc gcccacccgc ggagcaaccg
     3901 ggaggtttcg gccggcgtgg cgtcggtcgc gaagtcggcg tcgacaccgg gtgtcagccg
     3961 gcccagcacc aggtcacgga ccgctccccc gacgaggtac agctcgtgac cggccgtctg
     4021 gaaacggtcg ccgagttcct tcgcgagcgg atcgacctcg agggtggtca cgacgtccaa
     4081 ccttaccctc gcgctcctcc cgccccggcg gagtacggtg cggcgatgcc cgtctggctc
     4141 gggttcctct ggacgctacc gaacacgctt ctcggactcg tgctcggtgc cttcacgctc
     4201 cagatccccc ggctcgatca cggggtggtc atcttcgatc atgccgcccg ggggctcacg
     4261 gcgctgctgc cacgtctcgg tcggaccgcg atgactgtcg gcgtcgtgat cgtgtcggcc
     4321 cggccgctcg agggaagact cctggcccac gagcgccagc acgtgcgtca gtacctcgag
     4381 tggggcccgt tcttcatccc cgcgtacctc gtggttgcgg tccgctacgg gtaccggcac
     4441 catccgatgg aactcgccgc cgtccgcgca gcggaagcgg cggagccgta gccgatcagt
     4501 acgggaggct ctcggaggcc gccgtcgcct cgtcgaacgc cgcgcggcgt tcgagcgtgc
     4561 cgtgcagggc caggtcgaag tcgtgagggt tggtcgcggt ccggcgtgcc tcgtccggcg
     4621 agacgacgcc ctccttcacg agcttcacga gcgcttggtc gaaggtctgc atgccgtaga
     4681 actccccgcc ctggatcacc tcgaggatgc ccgacgtctc atcggggtcc acgatcctgt
     4741 cgaagacgcg tccggtgttc accagcactt ccacgaccgg gacgcgcccc ttcccgtcgg
     4801 cacggctcac gagccgctgg gacacgatcc cgcggagagc gccggcgaag gaggagcgga
     4861 tctctcgctg ctgctcgggc gggaacaggt cgagtgtccg gttgatcgtc tccatcgcgt
     4921 cgatcgtgtg cagggtggag atcacgaggt gtccggtctc ggccgcctgg atcgcggcga
     4981 gcgccgagtc cgcgtcacgg atctcaccga tgaagatcac gtccgggtcc tgacggatga
     5041 cgtgccggag ggcggccgcg tacgactccg tgtcgatccc gatctcgcgc tgctggacga
     5101 tcgacagctg gtcgtcgtgg acgacctcga tcggatcttc gacggtcacg atgtgagctc
     5161 ggcgggtgcg gttgatgtga ccgatcatcg cggcgatggt cgtcgtcttc ccggttcctg
     5221 tcggacccgt gatcaggatc aggccgcgtg gggaggccgc gagcgtgctc atcacgggag
     5281 gcaatcggag ctcctcgaac gacggcacct cggcgcggac gcgacggatc gcgagcccga
     5341 tcacgccgcg ctggcggaag acgttgacac ggaaccgtcc cacgccggag agggtgtagc
     5401 ccaggtcggc ctcgttcgtc tgctcgaact cccgcttctt gtgctcgctc atgagggcgt
     5461 gggcgaaccg gtcggtgtcg gcagcggaga ggtagccgag ctcggccggc gagagctcgc
     5521 cgtccacgcg cacgaacggg acgttgcccg ccttcaggtg gagatcgctc gcgccccgct
     5581 cgaccgtgta ccgcagcagc tcatcgatgt ccatgccaga ccgacgctcc tcttctcctc
     5641 gctggtcgcg cgcccttcgg aggctcggta catgcatcgg cccctcccgc cggggacctg
     5701 aggaacggaa cctagaatca ggccggatgg ctgagggagg cggagcgtcg acgccgagcg
     5761 cggatgccgg cggctccaag cgccgtcgca ggcgacggcc gagacgcggc accgcgcacg
     5821 cggccggcga cgcgaaggca cagggggcga tccggggtgg cgatgcggca ggcgatgccc
     5881 ctcgatcgtc ggcgaggacc gatcgagccg gtgccgcggc tggacgcatc cacgcggaga
     5941 cgggccaggg tcagaagcgc aaggctcaga ggaagagccc gaggaaggcc cagaagaagc
     6001 agccgaagaa ggagcagaag agccagaagc agcggcagaa gcggggcggc acgtcgggaa
     6061 agccttcccg gagaacccgt ggcgccccca ccaagcgaga gatctccgcg ggcggggtgg
     6121 tctaccgcag gggcggcgac gagatcgaga tcgtcctggc gtcacgacgc actcgccgag
     6181 gagagctcgc ctgggggctc gcgaagggcg gcatcgaaga caacgaatcc cctgaagaag
     6241 cggcggttcg agaggtccgg gaggagacag ggctcttcgc ggagatcgag gactccctgg
     6301 gggagacccg ctacttctac gtgtgggagg acgtacggat ccgcaagacc gtccatttct
     6361 tcttgatgcg gcacacgggc gggaacatcg acgaccgtga tgacgagatg gaggagatcc
     6421 gctggttccc gctcgagcgc gcgctcaagc gggccgcgta ccg
//
LOCUS       DASUYD010000223        16901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_819734, whole genome shotgun sequence.
ACCESSION   DASUYD010000223 DASUYD010000000
VERSION     DASUYD010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16901
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819734"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VF195_11725"
     CDS             <1..522
                     /locus_tag="VF195_11725"
                     /inference="COORDINATES: protein motif:HMM:NF016328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Coenzyme F420 hydrogenase/dehydrogenase, beta
                     subunit C-terminal domain"
                     /protein_id="HEX6581526.1"
                     /translation="ALEFRRVNKWRRKIAWTFGLLCSKSFTYDGLMVEIAQKELGLDL
                     DHLARVNVKGKLLFYTDTGDEVVYPLKEAHRFTRPGCLRCPDFAAEHADISFGGLGQS
                     EGWTLSIVRTDRGQDIWDRATADGVIEHRPGAEDPKAVELMFKLAAKSRERWPTSEEM
                     PAAGTTPGVLPLV"
     gene            672..3581
                     /locus_tag="VF195_11730"
     CDS             672..3581
                     /locus_tag="VF195_11730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX6581527.1"
                     /translation="MARSIRYQEPLLAGDGMLSAVAYSAERLLLSPDWRDALDDVLMH
                     LGVAAGVSRAYLIQVDPPDTGYRATQLAEWCAPGVSSQFENPTLQGASLADAGFSRWI
                     GLMSSRAIVHGIVNTFPEEERVELTDQEIRSIAAFPVFVNGEWWSFVGFDDCVDEREW
                     TAFELDSLRAAAGMLGAAEQASRAETRRRDAEIRYQQVVEQSPAVTYTESHHPEGGRF
                     TFISPQLNELLGSAPELPLGDRDWWWSRVHPEDRDEVQLANRLSFSRGTEFDQTYRMR
                     RADDSWAWVRDKVRPVREEDGRILYWQGFLVGVTDRVETEERLREAEARSRAMVERIP
                     AVTYTDHVGTDGITVMGFVSPQIEEILGYPPQRFLDRGDLWFEIMHPDDLARLRAIDA
                     FNNADLEPFEHEYRMRHVDGHWVWVHDRSTAVLDDDGNLDYFLGFLTDVSSRHDAEAR
                     LREAELTFRTMVEQNPAVFYIQGIDPDDPTKLLTRYIGPRAEQLTGVPVEEMIADPDL
                     WRTMIHPEDRERVFEADASSNADGSDRFALEYRVIRKDGSTVWVLDEATLIRPENGAP
                     YWQGFQLDITGRKEAEQRLRDAHEHLRVMVDSALDAVVSMNVHGVITAWNPQAETTFG
                     WSAEEVVGRSLVETIVPHAHRAAHSEGLRRWRETGEGSVLNARIEIQALHRDGRLIPV
                     ELAIVPVAVGESTSFSGFIRDISDRKRAQEDLERALEVEREAAVRLRALDEMKDTFLQ
                     AVSHDLRTPLAAILGLAITLERGDVGLLPEETRELAGRIEHNARRLERLVTNLLDLDR
                     LARGVVTPAFEPTDVGDLVRRMVTESDPSLRDQVTVSAESVVVPIDPPKVERIVENLL
                     VNAVRHTPAGTSVHVSVTAITHGAMILVEDEGNGVPHDLRERVFEEFSQGTDAPQPSP
                     GVGVGLTLVRRFAEMHHGRAWVEEREGGGASFRVFLPAVHPEEAAPLQPAVSLRG"
     gene            complement(3578..3904)
                     /gene="fdxA"
                     /locus_tag="VF195_11735"
     CDS             complement(3578..3904)
                     /gene="fdxA"
                     /locus_tag="VF195_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017837128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX6581528.1"
                     /translation="MTYTIAEPCIDVKDRGCVDECPVDCIYEGPRMLYIHPDECVDCG
                     ACEPVCPVTAIFYEDDVPAEWKQFNAINAEWFSDDISGVGSPGGAAQVGPATKDHPVV
                     EAWPTL"
     gene            complement(3977..4852)
                     /locus_tag="VF195_11740"
     CDS             complement(3977..4852)
                     /locus_tag="VF195_11740"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6581529.1"
                     /translation="MGELRQPTGTAFTIRDPLSWSDVAAITRAGEALGYAAVFLPETG
                     SRDTLATLTGLADETSSLLLGTGVIPIGSRTSKLAAMAAATVQERSAGRHILGVGTGG
                     SAPGALGRLRTYVEEIRGLVGGSAPGDPLRLPLPAPVPIWIAALGPKATNLAGAIADG
                     VILNWCTPERVAEARDAIRASAAEAGRGPGACTISVYVRASFTDRADEALLAAAGEYT
                     SYPSYARQFEAMGVEPTARGTVDAVCLRGDPAAAKDRLEAYRVAGADLPAVYPVLAPG
                     EGSEASLAVLEALAP"
     gene            4875..5528
                     /locus_tag="VF195_11745"
     CDS             4875..5528
                     /locus_tag="VF195_11745"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX6581530.1"
                     /translation="MEAVTFLTEDGVRLEGELRMPDDPPRGSAVICHAHPKHGGSKDH
                     PVLWSVRNELAGKRGRATLVFNFRGTMGSAGTYGGGHDELRDVRAAIGTVRERAEGPT
                     LVFGWSFGASVALREAVDDERTSALALFGPPLRPNDLRLPPMPSAAELRLLKRPLLLL
                     AGANDEYCPAGDLRTYGEGVAEVVIMDGTDHYLWRRELEAAAIVGDFADRVTSSAPT"
     gene            complement(5569..5730)
                     /locus_tag="VF195_11750"
     CDS             complement(5569..5730)
                     /locus_tag="VF195_11750"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581531.1"
                     /translation="MGDDRLIGGGGSDVLHGDTGDDLLRGGPGRDRLVGGAGTDTCVG
                     GTVEIGCEA"
     gene            complement(5712..6017)
                     /locus_tag="VF195_11755"
     CDS             complement(5712..6017)
                     /locus_tag="VF195_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581532.1"
                     /translation="MGLVLALGIIAYDVISLQASHRRCFHQAGVAGDEQDNAMEGTPG
                     NDVLLGPGGSDAISGSGGDDRLCGNAGQDRIWGDVGADRLAGNGRRDRLEGGWVTTG"
     gene            complement(6108..6488)
                     /locus_tag="VF195_11760"
     CDS             complement(6108..6488)
                     /locus_tag="VF195_11760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581533.1"
                     /translation="MEARDVLLVLPFLVLIMLGLGFVLVFRERAAAQEVLRRRAQLAE
                     PDLTTPLRDSRPPERPWWGNPWLWVLVSSASVVLGIFVWPGLFGGTFLFLPFIWVWRP
                     RRGREGDPRSNGHGSRVDPGSLGG"
     gene            complement(6497..7924)
                     /locus_tag="VF195_11765"
     CDS             complement(6497..7924)
                     /locus_tag="VF195_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019608011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6581534.1"
                     /translation="MAVRPYLVNGEWRTGDGSFEVHSPYDDSVVAEIGVPTDADVEEA
                     AATAHETFEESSKLPVHARAEALDHVSKRLGETIEENAKLIAQEGGKPLKWATVEATR
                     AVSTFRWASEVIRRGDDELIRLDTEAALGSRVGLIRRFPIGPVLGITPFNFPLNLVAH
                     KVAPSLGVGAPIVVKPASATPIGSLRLAEFFAEADLPKGMYQVLPVSSKVADGMARDE
                     RYRKISFTGSAEIGWYLKGLDPKKRVTLELGGNAGVIVHADADLDFAAQRIAFGGYYQ
                     AGQSCISVQRVLVSSEVYDDFTARLVKQVESLKVGDPMDPTVDVGPVIQRSEVDRIAG
                     WVKEAISEGAEVLTGGEGDGPFFQPTLISKVTPEMKVCREEVFGPVVTISPYQTFDEA
                     LKIVNDSKYGLQAGVFTNDINRAFEAHRSIEVGGVIVNDVSAFRADQMPYGGSKDSGF
                     GREGLRYAMEEMTEPRILVLSHVPL"
     gene            complement(7957..9393)
                     /locus_tag="VF195_11770"
     CDS             complement(7957..9393)
                     /locus_tag="VF195_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007577211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-aminobutyraldehyde dehydrogenase"
                     /protein_id="HEX6581535.1"
                     /translation="MADAYQMFIDGEWVNSSDGQTRDVINPATGEVIASVQEGTKEDV
                     DRAVAAAKKAFEGDWYDSTPKDRQLALLKLADRIEEHADEIVKLEAQNVGKPAAVTLS
                     EEIPPIVDNLRFFGGAARTMEGRSAGEYMAGFTSYVRREPIGVAGLIAPWNYPLMMAI
                     WKIGPALATGNAIVLKPSEMTPLTALKLAELTADILPPGVFNVVTGDGVPVGDRLVRH
                     PDVGIVSLTGDTATGKLIAANAADTMKRVHLELGGKAPFIVFDDADMEQVVEWIKVGG
                     YFNSGQDCTASARILVGSGAYENVLAELVPAVESIKVGDPFADDTEMGSMVSKEQLER
                     VVGFVTRAEEAGAKVLTGGKAIDQEGYWYSPTVVSEVGQDAEIIQKEVFGPVVTVQRF
                     DDEAQALEWANGVPYGLSSSVWTRDVGRAHRMARRLQFGCVWINTHIPLTPEMPHGGY
                     KQSGHGKDMSVYSLEDYTNMKHVMVSLD"
     gene            complement(9538..10506)
                     /locus_tag="VF195_11775"
     CDS             complement(9538..10506)
                     /locus_tag="VF195_11775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581536.1"
                     /translation="MLRYIARRLLYSVLVLVIISILTFLIFVKLPAGDPARRAVGRRT
                     DPQQIANAREAFGLDRPLIVQYGRFAKGLIPLPNTFLSEDVYYSFSNYLPVREEIWRR
                     LPVSAILGTGAAVLWLLIGIPLGIASGVRPRSLSARWTMVLAIVGVSLPVFWLGQLLL
                     YVVWYKLHLAPSAGLEINASVWGSALNGKFLLPWVTVAVGYAAIYARMLRSNMVETTQ
                     EDYIRTARAKGLSERRVVYKHALRGALTPIVTMLGIDFATILAGLVITETLFGLPGLG
                     QLAVRSIYSNDFPMVMGVTVVGSMFILAANIVVDIAYAFLDPRVRY"
     gene            complement(10645..11160)
                     /locus_tag="VF195_11780"
     CDS             complement(10645..11160)
                     /locus_tag="VF195_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581537.1"
                     /translation="MRRREARPRSRKDPEALPAAVRDTPRMPSDGPSGPNALDFWLGE
                     WSCTWDGGLGRNSITKELGDAVVVERFESLEPERWSGMSVNVFDELHGWRQTWVDSTG
                     NYWALQGRSHPEGFAFDVDEVEGGRGITKRMVFSDIQADAFRWRWERSEDGGTTWSEL
                     WVIDYRRAHDA"
     gene            11261..12607
                     /gene="gabT"
                     /locus_tag="VF195_11785"
     CDS             11261..12607
                     /gene="gabT"
                     /locus_tag="VF195_11785"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HEX6581538.1"
                     /translation="MDIPQERKLVTEIPGPRSREWFERREIAVPRGVANIHPIVTARA
                     SGAIVEDVDGNRLIDFATGIAVLNVGHTAPEVVLAAQRQLELDTHTCFHVTANEPYIE
                     LAERLNALAPGDHEKKTMFANSGAEAVENAVKIARKHTGRPGVVVFDHAFHGRTLMAM
                     SLTAKVMPYKQGMGPFAPEVFRLPLAYPYRCPTGGTEETCADSCLAYALDEMHKHIGQ
                     ENIAAILIEPIQGEGGFIVPAPGFISGLAEFAAANGIVFIADEIQSGMGRAGRWFAIE
                     DDGVVPDIVLSAKSLGGGLPISAVTGRADVMDSVHVGGLGGTYGGNPVAAAAALAVLD
                     KIEAEGLLERSRSVGGTIHGRMRAMQDLFDSVGDVRGRGAMRAMELVADRSTKEPLDG
                     EVMSALGRAALERGLIVLTAGTYGNVLRLLPPVTIDVGLLDEGLDLLEEAVAATAG"
     gene            12627..13895
                     /locus_tag="VF195_11790"
     CDS             12627..13895
                     /locus_tag="VF195_11790"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6581539.1"
                     /translation="MSDPPPRTSEGRLRRLAIDLGPLRASREFRLLWTGLLVTATGSQ
                     FTLVAVFVQVTNLTDSEAAVGATGLAYLLGLVAGSLGGGAILDAWDRRRLLMMAQVGL
                     AAGSTVLLTGALVGEPPLWVIYSGLAVLAASSSFDGPTRSAITARILEPAQLPAAQAL
                     NQVVWNTAALIGPLLAGLVIVWWDVTVAYAIDLASIGCMFVAASLLRPMPPVREDHAD
                     TGLRAIREGFRFARKHRLIMATFVIDLIAMIFGLPRALFPFLIVDQFHHSSKAIGLLF
                     AALAVGALLGALTSGWTGRVHRQGLAVIVSVAAWGVAIAAFGWSNTFWVGLAMLALAG
                     WADVISAIFRTTILQMSAPDRLRGRLFGIHILVVTGGPRLGDVEAGVVARLASPTFSV
                     VSGGIACVLGAGLVALLYPELRRARAPHST"
     gene            13911..14201
                     /locus_tag="VF195_11795"
     CDS             13911..14201
                     /locus_tag="VF195_11795"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX6581540.1"
                     /translation="MAKLTEAELNTFLDGMQGWSSRSNAIHRDFTFPGFRSAITFVNR
                     VAELAETAGHHPDTEIHYNRVSLSLSTHDAGGVTEKDVALAAEIDLFAEPSA"
     gene            14207..15910
                     /locus_tag="VF195_11800"
     CDS             14207..15910
                     /locus_tag="VF195_11800"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEX6581541.1"
                     /translation="MAEAVRTAELRVLEGPNVYFPRPAVKLMLEVGGWLRASDDRVQE
                     VADRIGVPRLEPGTAGSDQRLRATARIAVQLTRRIAETVGTGRLAVRGRPGPEFGHVV
                     IAFPWRRRGAAEALAEAVATGMTALLRRSPARVIAELGRTVAAAEPGQPPTVPDPDVP
                     VIQVSGTNGKTTTTRLIAHMVMTAGKRVAFSSTDGVYRNDRLVKKGDYSGFAGAATAL
                     AQHPDVAVLETARGGILLRGIGVAHNDVAVVTNVSADHLHLHGISTVDQLAEVKATVT
                     RITRPDGWVVLNADDPRVLAMRRTASGRPWLFSLDPRHPAIRETLGGGGRAISVLDGT
                     IAWMEHSVVHPLIELDRVPVTLAGLSRMYTQNALAAAAAALAAGLSPRAVAKGLRTFV
                     LDPQTNPGRTNLFRLDRRLLVLDYAHNEAGMDGLVEILQGLRPPGREIWLAIGTAGDR
                     TDDILHGFAVRAALGSDHLAIAELKKYLRGRPREDVIAQLRLGAARAGKLDVPVYVDE
                     LRALRGMLKASSPGDVVGITALAQRAQAFRWLRTREAKLLGPADVRRLVRAAAGERSA
                     G"
     gene            15960..>16901
                     /locus_tag="VF195_11805"
     CDS             15960..>16901
                     /locus_tag="VF195_11805"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX6581542.1"
                     /translation="MATPQHSAMESALVVASNRGPVRFERDEDGRLVAHRGSGGLVTV
                     LGGVLERDDATWVAAAVTEGDREVAPRGRKLEGAAQRVRYVDIPEERYDGYYNGIANG
                     ILWFAHHYLWDIARTPTFDERTEHDWADYVEANRAFARALSVEAKHDPIFLIQDYHLT
                     LVPQLLREMVPDARILHFSHTPFAGRTYLRTLPVDMRQSILRGMLGADVVGFQGRQWG
                     ENFLLSARELEDVHVDIRAHRAEVDGRQVQIRSFPVAVGAQEIQEAAATDRAKRLRTQ
                     VEKIRGGRKLLLRVDRLEPSKNILRGFLAFELLLQKHP"
BASE COUNT         2591 a         5888 c         5792 g         2630 t
ORIGIN      
        1 gcgctggagt tccggcgggt gaacaagtgg cgtcgcaaga tcgcgtggac cttcggactg
       61 ctctgctcca agtccttcac ctacgacggc ctgatggtgg agatcgcgca gaaggagctc
      121 ggcctcgatc tcgatcacct cgcacgcgtg aacgtgaaag gcaagctgct cttctacacc
      181 gacaccgggg acgaggtcgt ctaccccctg aaggaggcgc accggttcac ccgccccggc
      241 tgcctgcgct gccccgattt cgccgccgag catgccgaca tctccttcgg tggcctcgga
      301 cagtcggagg ggtggacgct gtcgatcgtg cgcacggacc gtgggcagga catctgggat
      361 cgggcgacgg cggacggcgt gatcgagcat cggcccggag cagaggaccc gaaggcggtc
      421 gagctgatgt tcaagctcgc ggcgaagtcc cgggagcggt ggccgacctc cgaggagatg
      481 cccgcggcgg gcaccacacc cggggtcctc ccgctggtct gaggaaaggc ccgtccttcc
      541 tgaccagacc tgggcccccg gacggccgac gtccgggccc caaccagggg actccatgcc
      601 accctcggag cgcccgataa tcccaccagg ccctgaacgg gtcgcactct gccgctcctc
      661 cgggggatcg cgtggctcga tcgatccgat atcaagagcc gttgctcgcg ggcgacggca
      721 tgctctccgc cgtggcctac tcggccgaac ggctgctcct gtctccggac tggcgggatg
      781 cgctcgacga cgtgctgatg cacctgggcg tggccgccgg cgtcagccgg gcctacctga
      841 tccaggtcga cccgccggac accgggtatc gggcgaccca gctcgccgaa tggtgcgcgc
      901 cgggtgtctc gtcgcagttc gagaacccca cactccaggg cgcgtccctc gccgacgcgg
      961 ggttctctcg atggatcggt ctgatgtcgt cgcgggcgat cgtgcatggg atcgtcaaca
     1021 cgttccccga ggaggaacgg gtcgagctca cggatcagga gatccgctcg atcgccgcgt
     1081 tccccgtgtt cgtgaacggg gaatggtggt ccttcgtcgg cttcgacgat tgcgtcgacg
     1141 agcgagagtg gacggcgttc gagctcgact cgctccgagc ggcggccggc atgctcggag
     1201 cggcggagca ggccagccgg gccgagaccc gccggcgcga tgcggagatc cggtaccagc
     1261 aggtcgtgga gcagagcccc gccgtcacct acacggagtc ccatcatccc gagggaggac
     1321 ggttcacgtt catcagcccc cagctcaacg agctcctcgg ctccgcaccg gagctcccac
     1381 tcggcgatcg tgactggtgg tggtctcgcg tccatcccga ggacagggac gaggtgcaac
     1441 tcgccaaccg gctctcgttc tcgcggggca cggaattcga tcagacctac cggatgcgga
     1501 gggcggacga ctcctgggcg tgggtgcggg acaaggtccg gccggttcgg gaagaggacg
     1561 ggcggatcct gtactggcag ggattcctcg tcggcgtgac cgaccgcgtg gagaccgagg
     1621 aacggctccg ggaggccgag gctcgatccc gggcgatggt cgaacggatc cccgcggtca
     1681 cgtacaccga ccatgtcggg accgacggga tcaccgtgat gggcttcgtg agcccacaga
     1741 tcgaggagat cctgggctac ccgccccagc gcttcctgga tcgaggcgat ctgtggttcg
     1801 agatcatgca tcccgacgat ctggcgcgct tgcgagcgat cgatgcgttc aacaacgccg
     1861 acctcgagcc cttcgaacac gaataccgga tgcgccacgt cgacggccac tgggtgtggg
     1921 tgcacgaccg gtcgaccgcg gttctcgatg acgacggcaa cctcgactat ttcctcggct
     1981 tcctgaccga tgtgagctct cgccacgacg cggaggcgcg tctccgggag gcggagctca
     2041 cgttccgcac gatggtcgag cagaacccag cggtcttcta catccagggc atcgaccccg
     2101 acgaccccac gaagttgctg acgcggtaca tcggcccccg cgccgaacag ctcaccggcg
     2161 tccccgtcga agagatgatc gccgatcccg acctgtggag gacgatgatc catccggagg
     2221 accgcgagag ggtgttcgaa gcggacgcat cgagcaacgc ggacggctcg gacaggttcg
     2281 ccctcgagta tcgggtgatc cgcaaggacg gcagcacggt ctgggtcctc gacgaggcga
     2341 cactcatccg ccccgagaac ggggcaccct actggcaggg gttccagctc gacatcacgg
     2401 gtcgcaagga agccgagcag cgtctgcggg acgcccatga gcacctgcgc gtcatggtcg
     2461 actccgcact ggacgccgtc gtcagcatga acgtgcacgg cgtgatcacg gcgtggaacc
     2521 cgcaagcgga gaccacgttc gggtggagcg ccgaggaagt ggttggccgg tcgctcgtcg
     2581 agacgatcgt gccgcacgcg catcgggcgg cccacagcga gggactccgt cggtggcgtg
     2641 agacgggtga gggctccgtc ctgaacgctc gcatcgagat ccaggccctc catcgggacg
     2701 gccggctgat cccggtggaa ttggcgatcg ttccggtcgc ggtcggggag tcgacgagct
     2761 tctccggatt catccgcgac atcagcgacc ggaagcgggc acaggaggat ctggagcggg
     2821 ccctcgaggt cgagcgggag gcagcggtcc gactccgcgc cctggacgag atgaaggaca
     2881 cgttcctcca agccgtctcg cacgacctgc gaacgccgct cgccgccatc ctcggtctgg
     2941 ccatcacact cgaacgcggc gacgtcggct tgctgcccga ggagaccagg gagctcgcgg
     3001 gtcgcatcga gcacaacgcc cgtcggctcg aacgcctggt gacgaacctc ctcgatctcg
     3061 atcggctcgc gcgcggtgtc gtgaccccgg cgttcgaacc gaccgacgtg ggcgatctcg
     3121 tccgtcgcat ggtgacggag tcggacccgt cgctccgcga ccaggtgacg gtttcggcgg
     3181 agtcggtggt cgtgccgatc gatcccccga aggtggagcg gatcgtcgag aatctgctcg
     3241 tgaacgccgt ccgacacacc ccggcaggaa cgtccgtcca cgtgtccgtc accgcgatca
     3301 cccacggggc gatgatcctc gtggaggacg aggggaacgg cgtcccgcat gacctgcggg
     3361 agcgggtctt cgaggagttc agtcagggca cggacgcgcc gcagccttca ccgggtgtgg
     3421 gcgtcggcct gacactggtg cgacggttcg cggagatgca ccacgggcgc gcctgggtcg
     3481 aggaacggga aggcgggggc gcgtccttcc gggtgttcct ccccgcggtg catccggagg
     3541 aagctgctcc tctccagccg gcggtgagcc tccgaggcta gagggtgggc caggcttcga
     3601 cgaccggatg gtctttggtg gccggtccga cctgagccgc gccccccggc gagccgacgc
     3661 cgctgatgtc gtcgctgaac cactcggcgt tgatcgcgtt gaactgcttc cactcggcgg
     3721 ggacgtcatc ctcgtagaag atcgcggtga ccgggcacac gggctcgcag gcgccgcaat
     3781 cgacacactc gtcgggatgg atgtacagca tccgcgggcc ctcgtagatg cagtcgacgg
     3841 ggcactcgtc cacacagccg cggtccttca cgtcgatgca gggctctgcg atcgtgtacg
     3901 tcatgccatt tacccctttt ccacggtgtc gaccttgtga acggagtgta ccgccggccg
     3961 gagcttcagc aagtggtcat ggcgcgaggg cctcgagcac ggcgagcgac gcctcggagc
     4021 cttccccggg agccaggacc gggtagacgg cgggcagatc ggcgccggca acccggtagg
     4081 cctcgagccg gtccttcgcg gcagccgggt cgccgcggag gcatacggca tccacggttc
     4141 cccgggcggt cggctcgacc cccatcgcct cgaactgacg agcgtacgac gggtacgagg
     4201 tgtactcgcc ggcggccgcg aggagcgcct cgtccgcccg gtcggtgaac gaggctctga
     4261 cgtagacgga gatcgtgcag gcgccggggc cgcgaccggc ctcggccgcc gaggctcgga
     4321 tcgcgtcgcg ggcttctgcc acacgctccg gcgtgcacca gttcaagatc acgccgtcgg
     4381 cgatcgcacc cgcgaggttg gtcgccttcg gtccgagcgc cgcgatccag atcgggaccg
     4441 gggcgggaag tggcagccgg agcgggtccc cgggggcgct cccgcccacc aggccgcgga
     4501 tctcctccac gtaggtccgg agccgcccga gcgctcccgg cgccgatcca cccgtcccga
     4561 ccccgaggat gtggcggccc gccgaccgct cctgcacggt cgcggcggcc atcgcggcca
     4621 gcttcgacgt ccgcgacccg atcgggatca cgccggtgcc gaggaggagc gacgaggtct
     4681 cgtccgcgag accggtcagc gtcgcgagcg tgtcgcgcga gcccgtttcg ggcaggaaga
     4741 cggctgcgta cccgagcgcc tcgccagcgc gcgtgatcgc cgcgacgtca gaccacgaca
     4801 gcgggtcccg gatcgtgaag gccgtcccgg tcggttggcg cagctccccc acggctggca
     4861 tcgtcgcaga tgcgatggaa gccgtgacgt tcctcaccga ggacggcgtt cgcctggagg
     4921 gcgagctccg gatgcccgac gatccgcctc gcggctccgc ggtcatctgt cacgcccatc
     4981 cgaagcacgg cggcagcaag gaccatcccg tgctctggtc cgtgcggaac gagctcgccg
     5041 gcaagcgggg gcgggcgacg ctcgtcttca acttccgcgg cacgatgggc tcggccggga
     5101 cgtacggggg tggccacgac gagttgcggg acgtgcgggc ggcgatcggg accgtgcgcg
     5161 agcgcgccga gggtccgacg ctcgtcttcg ggtggtcgtt cggcgcgagc gtcgcgctcc
     5221 gggaggcggt cgatgacgag cgaacgagcg cgctcgcgtt gttcggtccc cccctcaggc
     5281 cgaacgatct ccggctcccg ccgatgccgt cggcggccga gctgcggctt ctcaagcgac
     5341 cactcctgct cctggcagga gcgaacgacg agtactgccc cgccggcgac ctccgcacct
     5401 acggcgaggg cgtcgcggag gtcgtgatca tggacggcac cgaccactac ctctggcgac
     5461 gggaactcga ggccgccgcg atcgtcggcg acttcgccga ccgcgttacg tcgtcagcac
     5521 cgacgtgatg ctctcgatcc tgccatcttc acgaccagcg tggtcacatc aggcttcgca
     5581 gccgatctcc acggtaccgc ccacacatgt gtcggtgccg gctccgccga cgaggcggtc
     5641 gcggccgggt cctccgcgca ggagatcatc tcccgtatct ccatggagca cgtcactccc
     5701 gccacctccg atcagccggt cgtcacccaa cccccctcaa gtcgatcccg ccgtccattt
     5761 cccgcgagac ggtctgcgcc cacatcgccc cagatccggt cctggccggc gttcccacac
     5821 agtcggtcgt ctccgcccga ccccgagatg gcatccgagc cgcccgggcc gaggaggaca
     5881 tcgttcccgg gcgtaccttc catggcgttg tcttgctcgt caccggcgac gccggcttga
     5941 tggaagcaac ggcgatgaga cgcctgaagc gatatgacgt cgtacgcgat gatcccgagg
     6001 gccaggacaa ggcccatccc gagcatcctc ggatagatcc gacggctcct tccccgaggg
     6061 ccagctatcc gacccatcta cgcctcctca cccacctttc cgggccatca tccaccgagg
     6121 gagccggggt cgaccctgga accgtggccg ttcgaacgtg ggtcgccctc gcgaccgcgt
     6181 cgggggcgcc agacccagat gaacggcagg aacaggaacg tgccgccgaa gaggccgggc
     6241 cacacgaaga tgccgagcac gacggacgcc gaagacacga gcacccacag ccacgggttc
     6301 ccccaccacg gtcgctcggg gggtcgggag tcacggagcg gtgtcgtcag gtccggctcc
     6361 gccagctggg cccgacgacg caacacctcc tgcgcggccg cccgctcacg gaacaccaga
     6421 acgaacccca gacccagcat gatcagcacg aggaacggca acacgagcag gacgtcgcgg
     6481 gcctccacga tcccgcctaa agcggaacgt gcgaaaggac caggatccga ggctcggtca
     6541 tctcctccat cgcgtaccga agaccctcgc gcccgaaacc ggagtccttc gaaccgccgt
     6601 acggcatctg gtccgcgcgg aaggcggaga cgtcgttcac gatgacgccg ccgacctcga
     6661 tcgagcgatg cgcctcgaac gcccggttga tgtcgttcgt gaacacgccg gcctggagac
     6721 cgtacttcga gtcgttcacg atcttgagcg cctcgtcgaa cgtctggtac gggctgatcg
     6781 tcacgaccgg tccgaagacc tcctcgcggc acaccttcat ctcgggcgtc accttcgaga
     6841 tcagcgtggg ctggaagaac ggcccgtcgc cttcgccgcc ggtgagcacc tcggcgccct
     6901 cggagatcgc ctccttgacc catcccgcga tccgatccac ctcggagcgc tggatcaccg
     6961 ggccgacatc gacggtgggg tccatcgggt cgccgacctt caggctctcg acctgcttca
     7021 cgagccgcgc cgtgaagtcg tcgtagacct cggaggagac cagcacgcgc tgcacgctga
     7081 tgcagctctg ccctgcctgg tagtagccgc cgaacgcgat ccgctgcgcc gcgaagtcga
     7141 ggtccgcgtc cgcatgcacg atcacgcctg cgttcccgcc gagctcgagc gtcacccgct
     7201 tcttcggatc gaggcccttc aggtaccagc cgatctcggc ggagccggtg aacgagatct
     7261 tccggtagcg ctcgtcgcga gccatcccgt cggccacctt cgaggacacg ggcagcacct
     7321 ggtacatccc cttcgggaga tcggcctcgg cgaagaactc cgccagacgg agcgagccga
     7381 taggggtcgc gctcgccggc ttcacgacga tcggcgctcc tacgccgagc gacggcgcca
     7441 ccttgtgcgc gacgaggttc agcgggaagt tgaagggcgt gatcccgagg accggtccga
     7501 tcgggaaccg ccggatcagc cccacccgcg agccgagcgc ggcctcggtg tccaggcgga
     7561 tcagctcgtc gtccccgcgc cggatcacct cggaggccca gcggaacgtg gacacggcgc
     7621 gcgtcgcctc gaccgtcgcc cacttgagcg gcttcccgcc ctcctgcgcg atcagcttgg
     7681 cgttctcctc gatcgtctcg ccgagccgct tcgagacgtg gtcgagagcc tccgcgcgcg
     7741 cgtggacggg gagcttcgag gactcctcga acgtctcgtg cgcggtcgcc gccgcttcct
     7801 cgacgtcagc atcggtgggg acgccgatct ccgcgacgac gctgtcgtcg tacgggctgt
     7861 ggacctcgaa cgaaccgtcg cccgtccgcc actccccgtt caccaggtag ggcctcaccg
     7921 ccatgtcatc gccgcctttc caagaggcgc gctcgttcag tcgagcgaca ccatcacgtg
     7981 cttcatgttg gtgtagtcct ccaaggagta cacggacatg tctttcccgt ggccggactg
     8041 cttgtacccg ccgtgtggca tctccggcgt gagcgggatg tgcgtgttga tccacacgca
     8101 cccgaactgg agccggcgag ccatccggtg cgcgcggccc acgtctcgcg tccacacgga
     8161 gctggacagt ccgtagggca cgccgttcgc ccactcgagg gcctgcgcct catcgtcgaa
     8221 gcgctgaacg gtgacgaccg ggccgaagac ctccttctgg atgatctcgg cgtcctggcc
     8281 cacctcgctc acgaccgtgg gtgagtacca gtagccctcc tggtcgatcg ccttgccgcc
     8341 ggtgaggacc ttcgcgccgg cctcctcggc ccgcgtgacg aacccgacga cgcgctcgag
     8401 ctgctccttc gagaccatcg agcccatctc ggtgtcgtcc gcgaacgggt cgccgacctt
     8461 gatcgactcc accgcgggga ccagctcggc gaggacgttc tcgtaggcgc ccgacccgac
     8521 gaggatcctc gccgatgccg tgcagtcctg accggagttg aagtagccgc cgaccttgat
     8581 ccattcgacg acctgctcca tgtccgcgtc gtcgaacacg atgaacggtg ccttgccgcc
     8641 gagctccagg tgcacccgct tcatcgtgtc ggcggcgttc gcggcgatca gcttcccggt
     8701 cgcggtgtcg ccggtgagcg agacgatgcc cacgtccggg tgccgcacga gcctgtcgcc
     8761 cacggggacg ccgtcgccgg tgacgacgtt gaacacgccg ggcggcagga tgtcggccgt
     8821 cagctccgcg agcttcagcg ccgtgagcgg cgtcatctct gacggtttga ggacgatcgc
     8881 gttccccgtc gcgagtgccg gcccgatctt ccagatcgcc atcatcaacg ggtagttcca
     8941 gggcgcgatc agccctgcca ctccgatcgg ctcccggcga acgtagctcg tgaacccggc
     9001 catgtactcg ccggcgctcc ggccctccat cgtccgcgcg gcgcctccga agaaccggag
     9061 attgtcgacg atcgggggga tctcctcgga gagcgtgacg gctgccggct tcccgacgtt
     9121 ctgggcctcc agcttcacga tctcgtcggc gtgctcctcg atccgatcgg cgagcttgag
     9181 gagggcgagc tgccggtcct tgggcgtgga gtcgtaccag tccccctcga aggccttctt
     9241 ggcggccgcg acggccctgt cgacgtcctc cttcgtgcct tcctggaccg aggcgatgac
     9301 ctcaccggtc gcggggttga tcacgtcgcg ggtctgtccg tccgacgagt tcacccactc
     9361 gccgtcgatg aacatctgat aggcgtccgc catgggttcc ctcctcgctc ggccgcctgc
     9421 ctgcgcggct cctgcaccaa cgttcggctc gccagtatgc gacatccgtc ggttgcggcg
     9481 catcccgcca tccggccggc cggatgggtt ctcgcagaac tcctccgtgc gacctggtca
     9541 gtagcggacg cgtgggtcga ggaacgcgta cgcgatgtcg acgacgatgt tggcggcgag
     9601 gatgaacatc gaacccacga cggtcacgcc catcaccatg gggaagtcgt tgctgtagat
     9661 cgaccggacg gcgagctggc cgagaccggg gagcccgaac agcgtttcgg tgatgacgag
     9721 ccccgcgagg atcgtcgcga agtcgatgcc gagcatcgtc acgatcgggg tcagcgcccc
     9781 tcggagcgcg tgcttgtaga cgacccggcg ctcggagagc cccttcgccc tcgcggtccg
     9841 gatgtagtcc tcctgggtcg tctccaccat gttgctgcgg agcatccgcg cgtagatcgc
     9901 cgcgtagccg acggcgaccg tgacccaggg gaggaggaac ttgccattca gcgcgctccc
     9961 ccacacgctg gcgttgatct ccagccccgc cgacggcgcg aggtggagct tgtaccagac
    10021 cacgtacagc agcagctggc cgagccagaa gacgggcagg gacactccca cgatcgccag
    10081 gaccatcgtc catcgagcac tcagcgatcg gggtcgcacg ccggaggcga tgccgagtgg
    10141 gatcccgatg aggagccaga gcacggccgc ccccgtcccg aggatcgccg agacggggag
    10201 ccggcgccag atctcctcgc gcaccggcag atagttcgag aacgagtagt agacgtcctc
    10261 ggacaggaac gtgttcggca gcgggatcaa ccccttcgcg aaccgcccgt actggacgat
    10321 caggggccgg tcgaggccga aagcctcccg cgcgttcgcg atctgctggg gatccgtcct
    10381 ccgaccgacc gcccgccgcg ccggatcacc ggccggcagc ttcacgaaga tcaggaacgt
    10441 gaggatcgag atgatgacga ggacgaggac ggagtagagc agccggcgag cgatgtaccg
    10501 gagcacgtca tcgacgctag gcccaggctc ggaccgcgga tagcacgttc ttgccgaggg
    10561 gtgccgtttc cttgccgttc ctgctggttc cttgccttcg ccaccatcca ggggcgccct
    10621 ctccgggcgc cgtacctggt tcggtcacgc gtcgtgggcg cgccggtagt cgatgaccca
    10681 gagctccgac caggtcgtgc cgccgtcctc cgatcgctcc caccgccatc ggaaggcgtc
    10741 ggcctggatg tcggagaaca ccatccgctt cgtgatcccc cgaccgccct cgacctcatc
    10801 gacgtcgaag gcgaagccct ccggatggga ccgcccctgg agcgcccagt agttgcccgt
    10861 cgaatccacc cacgtctggc gccaaccgtg cagctcgtcg aagacgttga cgctcatccc
    10921 cgaccatcgc tcgggctcga gagactcgaa gcgttccacg acgaccgcat ctccgagctc
    10981 cttcgtgatc gagttccggc cgaggcctcc gtcccacgtg caggaccatt ccccgagcca
    11041 gaagtcgaga gcgttcgggc ccgaggggcc gtcggacggc atcctcggag tgtcgcgcac
    11101 cgccgccggg agcgcttccg ggtccttgcg gctccttggg cgagcctccc ttcgtctcat
    11161 cggggtggtc tcccggctga caacccggcg ggatccgcac gatactcccg gtcgacgcgg
    11221 gcgcactgcg tcgcgatgag ccggcggacg gaggcctgtc atggacatcc cccaggagcg
    11281 gaagctggtc acggagatcc ctggaccgag atcgcgcgag tggttcgaac gtcgcgagat
    11341 cgccgtccct cgcggcgtcg cgaacatcca tccgatcgtc acggcacgcg cgtcaggcgc
    11401 gatcgtggaa gacgtcgacg ggaaccggtt gatcgacttc gccaccggca tcgcggtcct
    11461 gaacgtcggt cacacggcac ccgaggtggt gttggccgcg cagcggcagc tcgagctcga
    11521 cacgcacacg tgcttccacg tcaccgcgaa cgagccgtac atcgagctgg ccgaacggct
    11581 gaacgcgctc gcgcccggcg accacgagaa gaagacgatg ttcgcgaact ccggagccga
    11641 ggccgtcgag aacgcggtga agatcgcgcg gaagcacacg gggcggccgg gggtcgtcgt
    11701 gttcgatcac gcgttccacg gccggacgtt gatggccatg tcgctgacgg cgaaggtgat
    11761 gccctacaag caggggatgg ggccgttcgc gcccgaggtc ttcaggctgc cgctcgcgta
    11821 cccctatcga tgccccaccg gcgggactga agagacgtgc gccgattcct gcctcgccta
    11881 cgcgctggac gagatgcaca agcacatcgg ccaggagaac atcgcggcca tcctgatcga
    11941 gccgatccag ggcgagggcg ggttcatcgt gccggcgccg gggttcatct ccggcctggc
    12001 cgagttcgcc gccgccaacg gcatcgtgtt catcgccgac gagatccagt cggggatggg
    12061 tcgcgccggg cgatggttcg cgatcgaaga tgacggcgtg gtccccgaca tcgtactgag
    12121 cgccaagtcc ctggggggcg gtctgccgat ctcggccgtc accggtcgag cggacgtgat
    12181 ggacagcgtg cacgtcggcg ggctcggcgg gacgtacggc gggaacccgg tcgcggccgc
    12241 cgccgcgctc gccgtgctgg acaagatcga ggccgaggga ctgctggagc ggtctcgctc
    12301 ggtcggcgga acgatccatg ggcggatgcg cgcgatgcag gacctgttcg actcggtcgg
    12361 cgacgtgcgc ggacgcggag cgatgcgggc gatggagctc gtcgccgatc gttccacgaa
    12421 ggagccgctc gatggcgaag tcatgtcggc cctcggcaga gccgcgttgg aacgaggtct
    12481 gatcgtgttg accgccggga cctacggcaa cgtcctccgg ctgcttccgc cggtcacgat
    12541 cgacgtcggc ctgctggacg aggggctcga tctactggag gaggccgtcg ccgcgacggc
    12601 gggatgagcg cgacccagtt gcactggtga gcgatccgcc tcctaggacc tcggaggggc
    12661 gactccgccg cctggccatc gacctcgggc cgctccgcgc gtcgcgcgag ttccggttgc
    12721 tgtggaccgg gctcctcgtg acggcgaccg ggagccagtt cacgctcgtc gcggtgttcg
    12781 tccaggtgac gaacctcacc gattcggaag cggccgtcgg tgccacgggg ctcgcgtatc
    12841 tgctcggcct cgtggcgggt tcgctcgggg gcggcgcgat cctggacgcg tgggaccggc
    12901 ggcgactgct gatgatggcg caggtaggac tcgccgcggg ctccacggtc ctgctcaccg
    12961 gcgccctcgt gggcgagccg ccgctgtggg tgatctactc cggtctcgcg gttctcgccg
    13021 cgagcagttc gttcgacggc cccacccgat ccgcgatcac cgcccggatc ctcgagcccg
    13081 cgcagttgcc ggcggcgcag gccctgaacc aggtcgtgtg gaacacggcc gcgctgatcg
    13141 gcccgctgct cgccgggctc gtgatcgtgt ggtgggatgt gaccgtcgcc tacgcgatcg
    13201 acctcgcctc gatcggctgc atgttcgtcg ccgcctcgct cctgcgaccg atgccaccgg
    13261 tgcgcgagga tcacgccgac accggcctcc gcgcgatccg cgaggggttc cgcttcgcgc
    13321 ggaagcaccg gttgatcatg gcgacgttcg tgatcgacct gatcgcgatg atcttcggtc
    13381 tgccgcgtgc gctgttcccg ttcctcatcg tggaccagtt ccatcactcg tcgaaggcga
    13441 tcggactgct gttcgcggcc ctggcggtgg gcgcgctcct cggggcgctg accagcggct
    13501 ggaccggacg ggtccatcgc caggggctcg cggtgatcgt ctcggtggcc gcatgggggg
    13561 tggcgatcgc cgcgttcggc tggtcgaaca cgttctgggt cggactcgcg atgctcgcgc
    13621 tcgccggatg ggccgacgtg atcagcgcga tcttccgtac cacgatcctc cagatgtcgg
    13681 cccccgatcg gttgcgaggg aggctgttcg ggatccacat cctggtcgtg accggcggcc
    13741 cccgtctggg agacgtcgag gcgggggtgg tggcgcggct ggcctccccg acgttcagcg
    13801 tcgtctcggg ggggatcgcc tgtgtcctcg gagccgggct cgtcgcgctc ctctacccgg
    13861 agttgcggcg ggctcgcgcg ccccattcca cctgacgtac gctgcgccag atggcgaagc
    13921 tgaccgaggc ggagctgaac accttcctcg acgggatgca gggctggagc tcgcgctcga
    13981 acgcgatcca tcgcgacttc acgttccccg gtttccgatc ggcgatcacg ttcgtcaacc
    14041 gcgtggccga gctcgccgag accgcgggac accatcccga caccgagatc cactacaacc
    14101 gcgtctccct gtcgctgtcc acccatgacg ccgggggcgt gaccgagaag gacgtggcgc
    14161 tcgcggccga gatcgacctg ttcgccgagc cgtcggcctg atagccgtgg ccgaagcggt
    14221 ccgcaccgcc gagctgcgcg ttctggaggg tcccaacgtg tacttccccc gtcccgcggt
    14281 gaagctgatg ctcgaggttg gtggatggct ccgcgcaagc gacgaccgcg tccaggaggt
    14341 cgcggaccgg atcggtgtcc cgcggctcga gccaggtacc gcggggagcg accagcggct
    14401 gcgtgcgacg gcgcggatcg cggtgcagct gacgaggcgg atcgcggaga ccgtgggcac
    14461 gggccggctc gccgtgcgcg ggcggccggg ccccgagttc gggcacgtgg tcatcgcgtt
    14521 cccatggcga cgacgaggag ctgccgaggc gctcgccgag gcggtcgcca ccggcatgac
    14581 cgcgctcctc cgacgttcgc ccgcgcgggt catcgcggag ctcgggcgca cggtggcggc
    14641 cgccgagccg ggacagccgc cgaccgtccc tgacccagac gtccctgtga tccaggtgag
    14701 cggcacgaac gggaaaacca ccaccacccg gttgatcgcc cacatggtga tgaccgccgg
    14761 taagcgcgtg gcgttctcct cgacggacgg cgtctaccgg aacgatcggt tggtgaagaa
    14821 gggcgactac tcggggttcg ccggggccgc gacggccctc gcgcagcatc ccgacgtcgc
    14881 ggtgctcgag accgcccgtg gaggcatcct cctccgcggg atcggcgtcg cgcacaacga
    14941 cgtcgcggtg gtgacgaacg tctccgcgga ccacctccac ctccacggca tctcgaccgt
    15001 cgaccagctg gcggaggtca aagcgacggt caccaggatc acccggcccg atgggtgggt
    15061 cgttctgaac gcggacgacc cccgggtgct cgcgatgcgg cgcaccgcga gcggccggcc
    15121 ctggctcttc tctctggacc cccgtcaccc ggcgatccgc gagacgctcg gcggcggcgg
    15181 ccgcgcgatc agcgtcctcg acggcacgat cgcgtggatg gagcactcgg tcgtccaccc
    15241 gctgatcgag ctcgaccgcg tgccggtgac cctcgccgga ctctcccgga tgtacacgca
    15301 gaacgcgctc gccgcggcgg ccgccgcgct ggcggcggga ctctcgccgc gggccgtggc
    15361 gaaggggctc cggacgttcg tcctcgaccc gcagacgaac ccgggccgga ccaacctgtt
    15421 ccggctcgat cggcgactgc tcgtcctcga ctacgcccac aacgaggcgg ggatggacgg
    15481 gctcgtcgag atcctccagg gcctcaggcc gccgggccgg gagatctggc tcgcgatcgg
    15541 cacggccggc gaccggacgg atgacatcct gcatgggttc gcggtgcgtg cggccctcgg
    15601 ctcggaccat ctcgcgatcg ccgagctgaa gaagtacctc cgtggccggc cgcgcgagga
    15661 cgtgatcgcg cagctccgcc tcggcgccgc gcgagccggc aagctggacg tccccgtcta
    15721 cgtggacgag ctccgcgccc tccgggggat gctgaaggcc tcctcccccg gcgacgtcgt
    15781 cgggatcacc gccctcgcgc agcgggctca ggccttccga tggctccgga cgcgcgaggc
    15841 gaagctgctc ggtcccgccg acgtccgacg cctcgttcgg gcggccgccg gcgagaggag
    15901 cgccggctga gggtttccct tcggggtgac ctcgggcgag cctgggagga tgggggggca
    15961 tggcgacgcc ccagcactcc gcgatggaat ccgccctcgt cgtggcatcc aaccgggggc
    16021 ccgttcgttt cgagcgtgat gaggacggca ggctcgtcgc ccatcgtggc tccggcgggc
    16081 tcgtgacggt actgggaggg gttctcgagc gggacgacgc cacgtgggtc gccgccgccg
    16141 tcaccgaagg cgaccgggag gtggcgccgc ggggtcgcaa gctggagggt gccgcgcagc
    16201 gcgtccgcta cgtcgacatc ccggaggagc gctacgacgg ctactacaac gggatcgcga
    16261 acgggatcct gtggttcgcg caccactatc tgtgggacat cgcgaggacg ccgacgttcg
    16321 acgagcggac ggagcacgac tgggccgact acgtggaggc gaaccgggcc ttcgcccgcg
    16381 ccctgtccgt ggaagcgaag cacgatccga tcttcctgat ccaggattac cacctgacgc
    16441 tcgtgccgca gctcctcagg gagatggtcc cggacgcgcg catcctgcac ttctcgcata
    16501 cgccgttcgc cgggaggacc tacctccgta cgctcccggt cgacatgcgg caatcgatcc
    16561 tgcgtgggat gctcggcgcc gacgtcgtgg ggttccaggg ccgtcagtgg ggcgagaact
    16621 tcctcctgtc cgcccgcgag ctcgaggacg tccacgtcga catccgcgcg caccgcgccg
    16681 aggtggacgg caggcaggtg cagatcaggt ccttcccggt cgccgtgggc gcgcaggaga
    16741 tacaggaggc ggccgcgacg gaccgggcga agcgcctgcg cacgcaggtg gagaagatcc
    16801 gcggcggccg gaagctcctg ctccgcgtcg accggctcga gccgtcgaag aacatcctcc
    16861 gcggtttcct ggcgttcgag ctcctcctgc agaagcatcc g
//
LOCUS       DASUYD010000224        12236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_602466, whole genome shotgun sequence.
ACCESSION   DASUYD010000224 DASUYD010000000
VERSION     DASUYD010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12236
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602466"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(893..1867)
                     /locus_tag="VF195_11810"
     CDS             complement(893..1867)
                     /locus_tag="VF195_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005843047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX6581543.1"
                     /translation="MRDIGLVGVPFSGKSTLFTALTRAGSHGGQANVAVVPVPDPRLG
                     VLTEMERSKKTVPAQVRFVDVPGGTSSAQGLAKLRESDALAVVVRCFGADRAPLRELA
                     DVRSELLLADLAVVESALEKGLKRAKGRPGPDVHALQRAKGALDTERPLRDAGLDDQE
                     LAHLKGIAALTLKPEVVIANLEEGDELPAELGGAVGVYASIEAETTEMEPEEARALLE
                     EFGVSEPGLERVIAAGYGALDLITFLTTGDDETRAWEVRRGAKAPEAAGVIHTDLERG
                     FIRAEVISYHELVAIGSMDAAKASGKLRVEGKDYVVQEGDILHVRFAV"
     gene            complement(2197..2592)
                     /gene="sodN"
                     /locus_tag="VF195_11815"
     CDS             complement(2197..2592)
                     /gene="sodN"
                     /locus_tag="VF195_11815"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013872465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HEX6581544.1"
                     /translation="MFEAFLKPQRTVHAHCDLPCGIYDPAQARIEAESVKACQEKYQG
                     SDDAIFKDRAVAIKEQRANLVKEHLWVLWTDYFKPEHLETYPQLHDLFWNATKEAGNA
                     KRSEDPAQGQKLLDAIDELSKVFWETKSA"
     gene            2591..3049
                     /locus_tag="VF195_11820"
     CDS             2591..3049
                     /locus_tag="VF195_11820"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HEX6581545.1"
                     /translation="MDPPPPTWGEQPPYHRAPRPLAALSAVATVAAIGVALFRWRPSR
                     IEVRGASMVPTLMPGDWALAVARHRFGRGDIVVVEHPGRPGYEMVKRITGMPNERVGD
                     RALTADEIWVEGDREDASTDSRHFGPVRLEQVKAKVVLVYWPKERRRRIG"
     gene            complement(3057..4493)
                     /locus_tag="VF195_11825"
     CDS             complement(3057..4493)
                     /locus_tag="VF195_11825"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX6581546.1"
                     /translation="MMCRGFLSVICLGTLVAVTGPASSAIASASLTVDTFADTFDGSC
                     ADADCSLRDAVASVDPGGTVRVPPGFYALDRSGTGPYAGDVDLSRPVTIVGTGETGSF
                     VDASALGDRVFDVSSDVSLRHLALLGGSPVGRGGVVRTTAGTLVVSRCTIVRGRADNG
                     GAVAVGNAATASIDRSWISDNRATARGGGLFLLGATVVSRSTVSGNRAAGGGGAFVGP
                     GDPLTIGNATVSRNVAVRGGGVRAIGDIELSFASIVANRAGVGGGVLISSESENSTAN
                     SVFARNRASDRGPLCARPLTSDGRNVADVRGCGLRALDDLTGVDPRVGILRQNGGSTP
                     THALKADSPARGRGSGCDPTDQRGAPRSACDSGAYELVLCRGRPVTIVGTPGPDEFSG
                     GLGRDVFLGRGGDDEFQGSLDVDRACGGKGDDHLIGGPGDDHLTGTAGRDLLLGEGGG
                     DLLIGGPGVDVCRGGGGHDVTRRCETAS"
     gene            4574..5371
                     /locus_tag="VF195_11830"
     CDS             4574..5371
                     /locus_tag="VF195_11830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581547.1"
                     /translation="MEQLPPPPVPLRPDLAPVEIEVPRRRRFPFWVTVVCVLLALGMG
                     AVAIAPLFEGREDPQGPGFLFLDRTDQGTPTRWDPCEPIHYVVNAELAPPGSIEDVHE
                     AARRMTAATGITFVYEGTTDEEASISRDVYQFDRYGDRWVPVLIAWSDPDASDIPFER
                     GDRIAAGVAVPVIPPTRLDDVYVSGWVAINADDPNPPGFVAPGQQGPVILHELGHLMG
                     LGHVRAAGELMHPSGGGVVDFGPGDREGLRQLGADGGCIQVMEPIGP"
     gene            5601..5831
                     /locus_tag="VF195_11835"
     CDS             5601..5831
                     /locus_tag="VF195_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013493634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEX6581548.1"
                     /translation="MDTITATVDAKGQSCPGPLVSLAKAIRDASSGALFELLATDPGS
                     KSDVPSWAELSGNELLETAESDGVYRYVIRKR"
     gene            5837..6412
                     /locus_tag="VF195_11840"
     CDS             5837..6412
                     /locus_tag="VF195_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009153583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HEX6581549.1"
                     /translation="MATTETDLTSELQLADLLRRVEVLEEADGKRIEKKNKLTIIAWG
                     GDLDRIWPTTILATTAAASGMESSVFFTFWGLFAIVKPDVRVTGSNWMQKMMSMMNPG
                     SAQKAKLSRYQFAGAGPVMFKRLADDHQVAKPEELIQIAQDLGVRLIPCQMTMDLLGL
                     KREELLEGLEEPIGAATAIAEMRDAAVSLFI"
     gene            6482..7747
                     /locus_tag="VF195_11845"
     CDS             6482..7747
                     /locus_tag="VF195_11845"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6581550.1"
                     /translation="MSRLADEMRKTFASMHISRNFRLFFAGQLISVTGTWVNWTAGAV
                     LVLKLTDSGVALGIEAALLFLPVLLFGVYAGSIADRFDKRRILVWSNAGYAVLAALQF
                     ALVATDVVQIWMVFVIAFLTGLILAIEPPTRQSFYVELVGEEHITNAVSLNSAVFTGT
                     RVLGGAIAGTMIDTFGLWSPFLLDAVSYVAVIAALLAMRIDELHAQRKAEREPGLVRA
                     GLRYVWSSQALRVPLVVMALVYLFTFNFSVLIPLQAYRSYRGSGIEIGWLYAALGLGA
                     FLAAIAMANRQTEPTLGRLAGFSIAFGAFLAVAGWAPTFVLGLIAMVPVGYASMLFAI
                     TANSTLQRFTRADMRGRVMALYTTIFLGSTAIGGPIAGWVGEFWGAPEAIAASGAIAV
                     GAGLFALRSRETAPASTEVPTADTAARSS"
     gene            complement(7716..8327)
                     /locus_tag="VF195_11850"
     CDS             complement(7716..8327)
                     /locus_tag="VF195_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581551.1"
                     /translation="MDERTKRTALIAGAVLLALLSIGSGALIALSDDEGASTPTTTSS
                     LTPSAEPSDEPSVPPDPTMPESPEPPGGESPVLEDGHHFVYVTGASRLEDGTSEVEFD
                     LAYFYVGGSAEREAAERDDEVLNGYYIVNENDRLRTLPLAEDVEVAYIPVDACCELQP
                     GNVDAWLEAVLETNQTDYGGADVPWWFTVEGGEITRIEQQYLP"
     gene            complement(8327..9871)
                     /locus_tag="VF195_11855"
     CDS             complement(8327..9871)
                     /locus_tag="VF195_11855"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6581552.1"
                     /translation="MNTGPGTLERIRGALASPDFRKLFAIRLVSQSSDGLFQSALVAS
                     VVFLSPEQQDTATGFLKATLVIALPYSILGPFVGVFIDRWRRRRILLSAPWVKAAAIG
                     LVLFDPIDQAVPFFAGALLIMSVNRFFLSTAAAVVPRLVPHEDLLVANSLATVGGTVA
                     LLAGVFAGGRVIDAADATAPVVAAAGIGWLAASWIASRIESSLKPHTKPEDPELLRHQ
                     LRRVGVEFSDGARRLLRSPRAIGPITSITLDQIGQGIVLTLSLVVFRHTFGEGVASFS
                     NLIGAGGVGVLAGILTVGGLEQRVSKPRIVAGAFVVGGIVLIAVSFLIRDWSILLASF
                     AVGLTFAWKKISVDTMVQESIPDGYRGRVFSVYDVVYNLARVIAAALAIPMIPRTRLD
                     VDGTLAVVGTAFLLWAPVIGRWYGRAPELEIRFVAGGRADETPRAIAWGGAEEPVELV
                     RTWREERDGQRRLAFRLALPDGTQLDVSRPEPDGPWRLDREDDRTPPGPGPAHIVSGE
                     PDERGP"
     gene            complement(9915..11045)
                     /locus_tag="VF195_11860"
     CDS             complement(9915..11045)
                     /locus_tag="VF195_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006543054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX6581553.1"
                     /translation="MSTVRIAIVGVGNCASSLVQGLEYYKDADPSAKVPGLMHVELGG
                     YHVGDVEVVAAFDVDARKVGKDVAEAIFTEPNNTIRFADDIPPTGVTVTRGRTLDGLG
                     AFYREVVEESDEAEADVVRILQDAEADVLVSYLPVGSEEAARFYAQCAIDAGVGFVNC
                     LPAFIASDPVWARRFGEAGVPIVGDDIKSQVGATITHRMLAKLFEDRGAELDRTYQLN
                     FGGNMDFMNMLERSRLTSKKISKTQSVQSQLDVPLDKDSIHIGPSDHVPWLEDRKWAM
                     IRLEGRNFGDVPISVEMKLEVWDSPNSAGVVIDAVRCAKLAKDRGIGGPLIGPSAYFM
                     KSPPVQFSDSAAHDMVESFILGNGHAEDQDWTRFFDVQSTEA"
     gene            complement(11042..11731)
                     /locus_tag="VF195_11865"
     CDS             complement(11042..11731)
                     /locus_tag="VF195_11865"
                     /inference="COORDINATES: protein motif:HMM:NF021886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX6581554.1"
                     /translation="MLELAVLGLLKERPMHGYQLSRELGDSLGGLWRVSYGSLYPTLR
                     RLERDAAIESEAGDVRGARRKKVYRITPKGEQIFIELLQESPQDTQTEDARFRMRLAF
                     FRYLPPETRIRLLERRRQALKERLSTIAESVRTGRGGTDDYGRALIEHNRAITESDIT
                     WLEQLIAAERAKNTPAGGLKRRRAALRERAGSSSEAIARGRAGSSSDAIAQGRTGSSS
                     EAIAQGKERIS"
     gene            complement(11814..>12236)
                     /locus_tag="VF195_11870"
     CDS             complement(11814..>12236)
                     /locus_tag="VF195_11870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX6581555.1"
                     /translation="VAIFELGVTAIAMRYRRIRMIALGVLLTGLGFGAFGILLTGIGM
                     AGAVFVWTFGEMFGSPSTAAFIADRAPAHLRGRYQSSLGLTYSLAFTIGPIGGTAIYE
                     WWPRGLWIACAVLGVAGAALALAGERYPAPAMVEPPAG"
BASE COUNT         1924 a         4209 c         4197 g         1906 t
ORIGIN      
        1 gggggccttc ggcccctcta tcgtccgacg tgcaggcgat cgcggaggtc ccgctggcgg
       61 acgatcgtgt cggccggagt gacggggaac cgtcactccg ggacgggcga ggcggtgccg
      121 ccgctcgagt ccgggacgat catcacgctc ggctacggag tcacgtgcgc cgacgggtcg
      181 gaggtgagtt tctggctgct tctgaccgtg caggacacgg agcccgccac ggaagagctg
      241 ggcatcgtcg tgtggatcta tggcctgggc gagcgctcca cggaggtgcc gattatcacg
      301 atgacctacg agggcgagac gaggcgaggc tgcatcgggg ccttcggatg gacgctcccg
      361 gacgggacga aggtcgacga ggcatccgga tgccaccagc atcctgcccc agtgctccta
      421 cgaaccactc ttcacggtgc cgccgggcgt accgatcacc ctcgaggtgc cgaccgcgac
      481 cgaggtgttc gcgacgcgga cgatgtcacc cttctacgga gggcgcgatg ggttcggagc
      541 gagcgtgcga tgacccggcg gcgggagggt gacttcaccc tgacgttcgc ggtggcgggc
      601 tcgccgagcg atcgcgacat cgaactcgat ttgcgcggag gaggatcgga tcgccttcac
      661 gactccagac ggaccgcggg tcctccccgg tgggtccgcc tacgtccgag ccaacgtggc
      721 gggattccag cagacagacg tgatagagca gatgacgagg gagccggatg gctccacaga
      781 gcgggacggg acgtggcagg tcgtccgaaa cggctcggtc atcgcggcag tggagttcgg
      841 atccctccgc ggagtggcat gccgggggtc ggggatcggc ggcgtcttgc cctcagacgg
      901 cgaagcggac gtgcaggatg tcgccttcct gcacgacgta gtccttccct tcgacccgaa
      961 gcttgcccga cgccttcgct gcgtccatcg agccgatcgc cacgagctcg tggtaggaga
     1021 tcacctcggc gcggatgaag ccgcgctcga ggtcggtgtg gatcacgccg gcggcctccg
     1081 gtgccttcgc gccgcgccgc acctcccacg cgcgggtctc gtcgtcgccc gtggtcagga
     1141 acgtgatcag gtcgagtgcc ccgtacccgg cggcgatcac ccgctcgagc ccgggctcgc
     1201 tgacgccgaa ctcctcgagc aacgcccggg cctcctccgg ctccatctcg gtggtctcgg
     1261 cctcgatcga ggcgtagaca ccgaccgcgc cgccgagctc ggccgggagc tcatccccct
     1321 cctcgaggtt cgcgatcacg acctccggct tcagggtcag ggccgcgatg cccttcaggt
     1381 gggcgagctc ctggtcgtcg agaccggcgt cgcggagcgg tcgttccgtg tcgagcgcgc
     1441 ccttcgcccg ttgcagcgcg tggacgtccg gaccgggccg acccttcgcc cgcttcaacc
     1501 ccttctcgag cgcgctctcc accaccgcga ggtcggccaa cagcagctcg ctccggacgt
     1561 cggcgagttc tcggagcggc gcccggtcgg cgccgaagca ccgcaccacg accgcgagcg
     1621 cgtcggactc gcggagcttc gccagccctt gagcgctcga cgtgccaccc gggacgtcga
     1681 cgaagcgaac ctgcgcgggc acggtcttct tcgaacgttc catctccgtc aggacgccga
     1741 gacgtgggtc ggggaccggg acgaccgcga cgttggcctg ccctccgtgc gatccggcac
     1801 gggtcagcgc cgtgaagagg gtggacttcc ccgagaaggg cacgccgacc agaccgatgt
     1861 cccgcattgg tcggccaacg tatcagccgg aagtcgggca actgtcgacc ttcgaccact
     1921 atgcgcagtg gggctcctgt ccgatgcttg cgcacgagac cggccggcgc cggggtgccg
     1981 ccagacccgc ctcggcgccg ccggttctct ttcaccccgt gaggggggtg aaggatcgga
     2041 ggacctgagc gtgcaccctc gggtgcgcgt cgccggcggg cggatgctcc gacggccccg
     2101 cccgtcggcc tcttctcttg cacgtggggt gggaacgctg aggggccggg ccgcggagcg
     2161 gcccggccct catgcaagtg ggggaactcc tcggcgctac gccgacttgg tctcccagaa
     2221 caccttcgaa agctcgtcga tcgcgtccag cagcttctgg ccctgcgccg gatcctccga
     2281 ccgcttggcg ttgccggcct ccttggtggc gttccagaag agatcgtgca gctgcgggta
     2341 ggtctcgaga tgttccggct tgaagtagtc ggtccacagg acccacaggt gctccttgac
     2401 gaggttcgcg cgctgctcct tgatggcgac cgcgcggtcc ttgaagatcg cgtcgtcgga
     2461 gccctggtac ttctcctggc aggccttcac ggactccgcc tcgatacgcg cctgtgccgg
     2521 atcgtagatg ccgcacggaa ggtcgcagtg ggcgtggacg gtcctctgcg gtttgaggaa
     2581 tgcttcgaac atggatccgc ctcctccgac gtggggtgag cagccaccct accatcgggc
     2641 cccgagaccg cttgcggcgc tgagcgccgt agcgacggtg gcggcgatcg gcgtggcgct
     2701 gttccgctgg aggccgtcca ggatcgaggt ccggggggcc agcatggtgc cgacgttgat
     2761 gcccggcgac tgggcgttgg ccgtggcgcg ccatcggttc gggcgcggag acatcgtggt
     2821 cgtcgaacat cccggccgcc ccggctacga gatggtgaaa cggatcacgg gcatgccgaa
     2881 cgagcgcgtg ggtgatcggg cgctgaccgc cgacgagatc tgggtggagg gtgatcgcga
     2941 ggacgcctcc accgacagcc gccacttcgg gccggtcagg ctggagcagg tgaaggcgaa
     3001 ggtcgtgctc gtctactggc cgaaggagcg ccggcgccgg atcgggtagg gagacgtcac
     3061 gacgcggttt cgcagcggcg cgtgacgtcg tgcccgccgc cgccgcggca cacgtcgacg
     3121 cccggacccc cgatgaggag gtcgccaccg ccctcgccga ggagcaggtc gcgacccgcg
     3181 gtgccggtga ggtggtcgtc gcccggaccg ccgatcagat ggtcatcgcc cttccctccg
     3241 cacgcgcgat cgacgtcgag ggaaccctgg aactcgtcgt cgccgccacg tccgaggaag
     3301 acgtcccgtc cgagaccgcc ggagaactcg tccggtccgg gcgtccccac gatcgtgacc
     3361 ggccggccgc ggcacaacac gagctcgtac gcgccgctgt cgcacgccga tcgaggcgcg
     3421 ccgcgttgat cggtcgggtc gcatccgctg ccccggcccc gggcgggact gtcggccttc
     3481 agggcgtggg tcggggtgga gccaccgttc tgtctgagga tcccgacccg cgggtccacg
     3541 ccggtgaggt catcaagggc cctgagacca cagcccctga catcggcgac attgcgtcca
     3601 tccgaagtga gcggtcgggc acacagcggg cctctgtcgg aggcacggtt gcgcgcgaac
     3661 acgctgttcg ctgtcgagtt ctccgactcc gacgagatga gcacgccacc acccacaccg
     3721 gcccgattcg ccacgatcga cgcgaaggag agctcgatgt cgccgatcgc ccgaacgcct
     3781 ccaccgcgga cggccacgtt cctcgagacg gtggcgttcc cgatcgtgag gggatcgccc
     3841 ggaccgacga aggcgccgcc tccccccgca gcgcggttcc ccgagaccgt cgatcgcgag
     3901 acgaccgtcg ccccgaggag gaagagcccg ccgccgcgag cggtcgctct gttgtcggag
     3961 atccaggagc ggtcgatcga tgcggtagcg gcgttcccca ccgcgacggc tcctccgttg
     4021 tccgcgcggc cgcggacgat cgtgcagcga ctcaccacga gtgtgcccgc cgtcgtccgg
     4081 acgacccctc ctcgtccgac ggggctccca ccgagcaacg cgagatggcg aagcgaaaca
     4141 tccgatgaca catcgaacac gcgatccccg agcgcggatg catcgacgaa gcttcccgtc
     4201 tcgccggtgc ccacgatcgt gaccggtcgg ctgaggtcga cgtcgccggc atacgggccg
     4261 gtcccggaac gatcgagtgc gtagaagccc ggtggcaccc gcacggtccc acccgggtcc
     4321 acggaagcga cggcgtcgcg gagcgagcag tccgcgtccg cgcagctccc gtcgaaggtg
     4381 tccgcgaacg tgtcgacggt gagggaagcg gaggcgatgg cgctcgacgc gggccccgtc
     4441 accgcgacca gcgtgccgag gcagatcacc gagaggaagc cgcggcacat catcgagaca
     4501 ccctacccac ggtccctgaa gccggaaccc gggcacgccg atgcttcaca cagagtcgac
     4561 ctaggatcga gagatggagc agcttccccc gccaccggtt ccgctcaggc ccgacctcgc
     4621 gccggtggag atcgaggtcc cgcgacgccg ccgtttccca ttctgggtga cggtcgtctg
     4681 cgtgctcctg gccctcggca tgggggcggt cgcgatcgct cccttgttcg agggcaggga
     4741 ggacccccag ggaccgggct tcctgttcct cgatcgaacc gatcagggga cgccgacccg
     4801 atgggacccc tgcgagccga tccattacgt cgtgaatgcc gagctcgcgc cgccgggatc
     4861 gatcgaggac gtccatgagg ccgcacgccg gatgacggcg gccacgggga tcaccttcgt
     4921 ctacgaaggg accaccgacg aggaagcgtc gatctcccgc gacgtgtacc agttcgatcg
     4981 atacggggac cgatgggtgc cggtcctcat cgcgtggtca gatcccgacg cctcggacat
     5041 cccgttcgag cgcggcgatc gcatcgccgc cggcgtcgcc gtcccggtca tccctccgac
     5101 ccgcctcgac gacgtctacg tgagcggatg ggtggccatc aacgccgacg atcccaaccc
     5161 accggggttc gtcgcgcccg gccagcaggg acccgtgatc ctccacgagt tgggccacct
     5221 gatggggctc gggcacgtgc gggcggccgg agagctgatg cacccctcgg gaggcggggt
     5281 cgtcgacttc ggtccgggcg atcgcgaggg acttcgccag ctcggggccg acggcgggtg
     5341 catccaggtc atggagccga tcggcccgtg atgggccttg ggaggcaagg gggatgcgcg
     5401 acgcagcggc gcagctcgac cttgccgaga tgggatcggt cgactaaggt gttgcacctg
     5461 cgaatggtgc gtacacgcga agccggcacc gtgggggcga tgatccccga ccgcatctgc
     5521 tgtcccgcct gaccgggacg gacccagact tccgcgccat cctgcaccgc ccgccctcag
     5581 agatcccagg aggtcccgcc gtggacacca tcaccgccac agtcgatgcg aaggggcaat
     5641 cctgcccggg gccgctcgtg tcgctcgcca aggccatcag agacgcgagc agcggcgccc
     5701 tgttcgaatt gctcgcgacc gatccgggct ccaagagcga cgtcccgagc tgggcggagc
     5761 tcagcggcaa cgagctgctg gagaccgccg agtccgacgg cgtctaccgc tacgtcatca
     5821 ggaagcggtg agtgtggtgg cgacgacgga gacggacctg acctcggagc tccagctcgc
     5881 ggacctgctc cgccgcgtcg aggtgctcga ggaagcagat ggcaagcgga tcgagaagaa
     5941 gaacaagctg acgatcatcg cttggggcgg tgacctcgac aggatctggc cgacgacgat
     6001 cctcgccacg accgccgccg cctccgggat ggagtcctcc gtcttcttca cgttctgggg
     6061 gctcttcgcg atcgtaaagc cggacgtgcg cgtcacgggc tcgaactgga tgcagaagat
     6121 gatgtcgatg atgaaccctg gctcggcgca gaaggccaag ctcagccggt accagttcgc
     6181 cggcgccggg cccgtgatgt tcaagaggct cgccgacgac catcaagtgg ccaagcccga
     6241 ggagctgatc cagattgcgc aggacctcgg tgtacgcctg atcccctgcc agatgacgat
     6301 ggatctcctc ggcttgaagc gggaggaact gctcgagggg ctcgaggagc cgatcggtgc
     6361 cgccaccgcg atcgcggaga tgcgcgacgc ggcagtgagt ctgttcatct aaggagcccg
     6421 gccgacctgc cggctaccag ccgggagaag gacggttcgt cccgcctcgt agtctggatg
     6481 catgtcccgg ctggccgacg agatgcggaa gacgttcgcc tcgatgcata tctcgaggaa
     6541 cttccggctc ttcttcgcgg ggcagctgat ctcggtcacc ggcacgtggg tgaactggac
     6601 cgccggcgcc gtgctcgtgc tgaagctcac ggacagcggc gtcgcgctgg ggatcgaggc
     6661 ggcgctcctg ttcctgccgg tcctcctctt cggggtctac gccggctcga tcgccgaccg
     6721 gttcgacaag cgacgcatcc tcgtctggtc gaacgccggc tacgccgtgc tcgccgccct
     6781 tcaattcgcg ctggtcgcca cggacgtcgt ccagatctgg atggtgttcg tgatcgcgtt
     6841 cctgaccggg ttgatcctcg cgatcgagcc cccgacgcga cagtcgttct acgtcgagct
     6901 ggtcggggag gagcacatca cgaacgcggt cagcctgaac agcgccgtgt tcaccggcac
     6961 ccgtgtgctg ggcggcgcga tcgccgggac gatgatcgac acgttcgggc tctggtcgcc
     7021 cttcctcttg gacgcggtgt cgtacgtggc ggtcatcgcc gccctgctgg cgatgcggat
     7081 cgacgagctc cacgcccaac gcaaggccga gcgtgaaccg gggctcgtcc gcgcgggcct
     7141 ccggtacgtg tggagctccc aggcgctccg cgtaccgctg gtcgtgatgg cgctcgtcta
     7201 cctgttcacc ttcaacttca gcgtcctgat accgctccag gcataccgga gctatcgcgg
     7261 ctccgggatt gagatcggtt ggctgtacgc cgcgctgggc ctcggcgcgt tcctcgccgc
     7321 catcgcgatg gcgaaccgac agacggagcc gacgcttggc aggctcgccg gcttctcgat
     7381 cgcgttcggc gcgttcctcg cggtcgcggg atgggcgccg accttcgtgc ttggactgat
     7441 cgcgatggtg cccgtcgggt acgcctcgat gctgttcgcc atcacggcga actccacgct
     7501 gcagcgattc acccgggctg acatgcgtgg ccgggtgatg gcgctgtaca cgacgatctt
     7561 cttgggaagc accgcgatcg gagggccgat cgccggatgg gtcggtgagt tctggggcgc
     7621 gcccgaagcg atcgcggcga gtggggcgat cgccgtgggg gccggtctgt tcgctctgcg
     7681 gtcgcgagag accgcgccgg caagcacgga ggttcctacg gcagatactg ctgctcgatc
     7741 ctcgtgatct ccccgccctc gaccgtgaac caccacggga cgtccgcgcc cccgtagtcg
     7801 gtctggttcg tctccaggac cgcctcgagc caggcgtcga cgttcccggg ctggagctcg
     7861 cagcacgcat cgacggggat atacgcgacc tcgacgtcct cggcgagcgg caaggtccgc
     7921 agccggtcgt tctcgttcac gatgtagtag ccgttcagga cctcgtcgtc gcgttcggcg
     7981 gcctcccgct ccgcggatcc acccacgtag aagtacgcca ggtcgaactc cacctccgag
     8041 gtgccgtcct ccagacggga agcgccggtg acgtacacga agtggtggcc atcctcgagg
     8101 acgggcgact caccaccggg cggctccggc gactcgggca tggtcgggtc cggaggcacg
     8161 ctgggctcgt cgctcggctc ggccgaaggc gtgaggctcg acgtcgtggt cggcgtgctc
     8221 gctccctcgt cgtcggagag cgcgatcagc gcccccgaac cgatcgagag cagcgcgagc
     8281 aggacggccc ccgcgatcag ggcggtccgt ttcgtacgct cgtccatcac ggccctcgct
     8341 cgtccggctc gccggacacg atatgcgccg gccctggtcc gggcggcgtc cgatcgtcct
     8401 cgcgatcgag acgccagggc ccgtccggct cgggacggct gacgtcgagc tgcgtgccgt
     8461 ccgggagggc gagacggaag gcgagccgcc gctgcccatc ccgctcttcc cgccaggtcc
     8521 gaacgagctc cacgggctcc tccgctccgc cccaggcgat cgcgcgtggc gtctcgtcgg
     8581 cgcgtccgcc ggcaacgaag cggatctcga gctcgggcgc gcgcccgtac cagcggccga
     8641 tcaccggggc ccacagcaag aacgcggtgc ccacgaccgc gagggtgcca tcgacgtcga
     8701 gccgagtccg ggggatcatc gggatcgcga gcgccgcggc gatcacgcgc gcgaggttgt
     8761 agacgacgtc ataaacgctg aacacgcgcc cgcgatagcc gtcggggatc gactcctgca
     8821 ccatcgtgtc cacggagatc ttcttccacg cgaacgtgag accgacggcg aagctcgcga
     8881 gcaggatgct ccagtcccgg atcaggaacg acacggcgat caagacgatg ccgccgacca
     8941 cgaacgcgcc ggccacgatc cgtggcttcg aaacccgttg ctcgagcccg ccgaccgtga
     9001 ggatgccggc caagacgccg acaccaccgg ctccgatcag gttcgagaac gaagcgaccc
     9061 cctcaccgaa cgtgtgccgg aacaccacga gggacagcgt gaggacgatc ccctgtccga
     9121 tctgatcgag ggtgatggac gtgatcgggc cgatcgcccg ggggctccgg agtaggcgac
     9181 gggctccatc cgagaactcc acgccgacgc ggcgcagctg gtgccggagg agctcgggat
     9241 cctcgggctt cgtgtggggc ttgagggagc tctcgatgcg cgaggcgatc cacgaggcgg
     9301 cgagccagcc gatgccggcc gcagcgacca ccggggccgt cgcgtccgcg gcatcgatga
     9361 cgcgaccgcc ggcgaagacc ccggccagca acgccacggt gccaccgacc gtggcaaggg
     9421 agttcgcgac gagcaggtcc tcgtgcggca cgagccgcgg gacgaccgcg gccgcggtcg
     9481 acaggaagaa ccggttcacc gacatgatca ggagcgcgcc cgcgaagaag gggaccgcct
     9541 ggtcgatcgg atcgaagagc acgagtccga tcgcggccgc cttcacccac ggggcggaca
     9601 gcaggatcct ccgtcgtcgc caccggtcga tgaagacgcc gacgaacggc ccgaggatcg
     9661 aataggggag cgcgatgacc agcgtcgcct tcaggaaccc ggtggcggtg tcctgctgct
     9721 ccggggacag gaagaccacg gaggcgacga gggccgactg gaacagcccg tcggaggatt
     9781 ggctgacgag ccggatcgcg aacagcttgc ggaaatccgg gctcgcgaga gccccacgga
     9841 tccgttccag cgtgcccggc ccggtgttca ccgagccgtc aggctatccg aggcgcggga
     9901 gccgactccg acgatcaggc ctccgtggac tggacgtcga agaaccgggt ccaatcttgg
     9961 tcctcggcgt gaccgttgcc caagatgaag ctctccacca tgtcgtgtgc cgcagagtcc
    10021 gagaactgga ccggcgggga cttcatgaag taggcgctcg gtccgatcag gggcccgccg
    10081 atcccccggt ccttcgccag cttcgcgcaa cggacggcgt cgatcacgac accggctgag
    10141 ttgggcgagt cccacacctc gagtttcatc tcgaccgaga tcgggacgtc gccgaagttc
    10201 cggccctcga ggcggatcat cgcccacttg cggtcctcca gccacgggac gtggtccgag
    10261 ggtccgatgt ggatcgagtc tttgtcgagc ggcacgtcga gctgcgactg gaccgactgc
    10321 gtcttcgaga tcttcttcga ggtcagccgg gaacgctcga gcatgttcat gaagtccatg
    10381 ttgccgccga agttgagctg gtacgtgcga tcgagctccg cgccgcgatc ctcgaacagc
    10441 ttcgcgagca tccggtgggt gatcgtggcg ccgacctgcg acttgatgtc gtcacccacg
    10501 atcggcacgc cggcctcgcc gaacctccgc gcccagaccg ggtccgaagc gatgaacgcc
    10561 gggaggcaat tcacgaagcc cacgccggca tcgatcgcgc actgcgcgta gaacctcgcg
    10621 gcctcctcgg agccgacggg caggtaggag accaggacgt cggcctccgc gtcctgcagg
    10681 atccgcacga cgtccgcctc ggcctcgtcg gactcctcga ccacctctcg gtagaaggcg
    10741 ccgagaccat cgagcgtccg accacgggtc acggtgaccc cggtcggcgg gatgtcgtcg
    10801 gcgaaccgga tcgtgttgtt gggctcggtg aagatcgcct cggcgacgtc cttgccgacc
    10861 ttcctcgcgt cgacgtcgaa cgccgcgacg acctcgacgt cgccgacgtg gtagccgccc
    10921 agctcgacgt gcatcagtcc cgggaccttc gccgaggggt cggcgtcctt gtagtactcg
    10981 aggccctgaa cgagcgatga ggcgcagttg ccgacgccga cgatcgcgat ccgaacggtg
    11041 ctcacgagat gcgctccttt ccttgggcta tcgcttcgct gcttgagccc gttctccctt
    11101 gggctatcgc gtcgctgctc gagcccgctc ttccacgggc tatcgcttcg ctgctcgagc
    11161 ccgctctttc acgcagcgcc gcgcggcggc gcttcagtcc ccccgcgggg gtgttcttgg
    11221 cacgttcggc tgcgatcagc tgctcgagcc acgtgatgtc ggactcggtg atcgcgcgat
    11281 tgtgctcgat cagcgcgcgc ccatagtcgt cggttccgcc ccggccggtt cgcaccgact
    11341 cggcgatcgt cgagaggcgc tccttgagcg cctggcgacg tcgttccagc aaccggatgc
    11401 gcgtctccgg tggcaggtag cggaagaacg ccaggcgcat cctgaaacga gcgtcctccg
    11461 tctgcgtgtc ctgcggcgac tcctgcagca gctcgatgaa gatctgctcc cccttgggcg
    11521 tgatccggta gaccttcttg cgcctggcgc ctcgcacgtc ccccgcctcg gactcgatcg
    11581 cggcgtcgcg ttccagacgg cgcagggtcg ggtagaggga accgtaggag accctccaca
    11641 ggccgcccag cgaatcgcca agctcacgtg agagctggta gccgtgcatg ggccgctcct
    11701 tcagcagacc caggaccgcg agttcgagca tgttcgagcg tcccttcacg tagcgcccgc
    11761 gatatatcgg cgcggtatat cggaccgata tatagcccag gagccccctg tcgtcaaccg
    11821 gcgggcggct caaccatcgc gggtgcggga taccgctcac ccgcgagcgc cagggcggct
    11881 ccggccacac cgagtaccgc gcaggcgatc cagaggcctc gtggccacca ctcgtagatg
    11941 gccgttcccc cgatcggccc gatcgtgaac gccaacgagt acgtgagccc caggctgctc
    12001 tgataccggc cccggagatg tgccggggct cggtcggcga tgaacgccgc ggtgctcgga
    12061 gatccgaaca tctcgccgaa ggtccatacg aagaccgcgc ccgccatccc gatccccgtc
    12121 aagaggatgc cgaacgctcc gaacccgagg ccggtgagga gcacccccag tgcgatcatc
    12181 cggatccgcc gataccgcat cgcgatcgcg gtgaccccga gctcgaagat cgcgac
//
LOCUS       DASUYD010000225         2130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_822450, whole genome shotgun sequence.
ACCESSION   DASUYD010000225 DASUYD010000000
VERSION     DASUYD010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822450"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF195_11875"
     CDS             complement(<1..372)
                     /locus_tag="VF195_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581556.1"
                     /translation="MDQPNDEQTEQGILKTVEAAGDVTVERALVGVVNGRDVTITQAG
                     AGPIAAQGDVNIQQGGCGPVLVGRDLTIRQGGCGPAMVRGSLSIEQGGTQSVLVAGPA
                     TIGRGAFVGLVASPKVTIDEGA"
     gene            414..1136
                     /locus_tag="VF195_11880"
     CDS             414..1136
                     /locus_tag="VF195_11880"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6581557.1"
                     /translation="MALNASSGRTFLLMGSGEFEPWSNEIEAAALHGRPGPVAVLPTA
                     SSAEGDDVFGRWGRMGLEHYAAAGIDARLVPVKTRQDADREDLARELEGAAMVFFSGG
                     KPQHLAETIHGTRLWDALLADLDAGTVYAGCSAGALIASQSREQRRERGVRTGWVYGL
                     GLVPHVSFGVHWDKVKVIPGLRSVVMSRIPPGSWFVGLDERTAILGDGRRWRVYGVGE
                     VTVRHAGGTKIHESGATFETRD"
     gene            1168..1470
                     /locus_tag="VF195_11885"
     CDS             1168..1470
                     /locus_tag="VF195_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581558.1"
                     /translation="MMSTEEPIAPEHQPTCYLCGRPTYDPDKRERPWVRATSGGHQVL
                     VCPSCQEERPDWAVQLDRCESCGATRLTAMLGQVVCRACGHVRGEPVEPAWMSTGA"
     gene            1461..1532
                     /locus_tag="VF195_11890"
     tRNA            1461..1532
                     /locus_tag="VF195_11890"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1493..1495,aa:Val,seq:tac)
     gene            complement(1538..>2130)
                     /locus_tag="VF195_11895"
     CDS             complement(1538..>2130)
                     /locus_tag="VF195_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX6581559.1"
                     /translation="DLGAEIVDPADIPHTDELEDPELEVFLFEFKTDLEAYLGSLGPS
                     QPFRTLAELIAFNDEHADVEMPFFGQERFEKAAEKGPLTEPAYVEALATCRRLSRGEG
                     VDAVMDEHRLDALVAPSNAPAWRIDYANGDHYVGGNSTPAAVAGYPNVTVPMGFAFDL
                     PIGLSFMGRGWSERTLIRHASAFERATRHRRPPRVA"
BASE COUNT          317 a          743 c          743 g          327 t
ORIGIN      
        1 cgcgccctcg tcgatggtga ccttcggcga cgcgacgaga ccgacgaacg ccccacgacc
       61 gatcgtcgcg ggaccggcca ccagcacgct ctgcgtgccc ccctgctcga tcgacaggct
      121 gccgcggacc atcgccggcc cacacccacc ctgccggatc gtgaggtcgc gcccgacgag
      181 cacggggccg cacccaccct gctggatgtt cacgtcgccc tgcgctgcga tcggcccggc
      241 tccggcctgc gtgatcgtca cgtcgcgccc gttcacgacg ccgacgagcg cgcgttcgac
      301 cgtcacgtct ccggcagctt ctaccgtctt caagatcccc tgttccgtct gttcgtcgtt
      361 cggctgatcc atccacgcca cctcctcagc cccatcgtag gtaccctcgc cggatggcgc
      421 tgaacgcttc gagcgggcga accttcctct tgatgggctc cggcgaattc gagccgtggt
      481 cgaacgagat cgaggccgcc gccctccacg gccggcccgg tcccgtcgcc gtgctaccaa
      541 cggcgagctc ggcggagggc gacgacgtgt tcggccggtg ggggaggatg gggctcgagc
      601 actacgctgc ggcggggatc gacgcacggc tggtgcctgt gaagacacgc caggacgccg
      661 atcgcgagga cctcgctcgc gagctggagg gcgccgcgat ggtcttcttc tccggcggca
      721 agccgcagca cctggcggag acgatccacg gcacgaggct gtgggatgcg ctgctcgccg
      781 atctcgacgc cggcacggtc tacgccgggt gcagcgcggg ggcgctgatc gcgagccaat
      841 cgcgcgagca gcgtcgcgaa cgcggtgttc gcacgggatg ggtctacggg ctcgggctcg
      901 tgccgcacgt ctcgttcggc gtccactggg acaaggtgaa ggtgatcccg gggctccgat
      961 cggtcgtgat gtcgaggatc ccgccgggct cgtggttcgt gggcctggac gagcggaccg
     1021 cgatcctagg tgacgggcgc cgctggcgcg tgtacggggt aggagaggtg acggtccgcc
     1081 acgccggagg gaccaagatc cacgagtcgg gtgcgacctt cgagacgagg gattagcgga
     1141 cccgccaggt ggatatcctt ctccaccgtg atgtcgaccg aggaacccat cgcgccggag
     1201 caccaaccga cctgctatct ctgcggccgg cccacctacg accccgacaa gcgggagcgg
     1261 ccgtgggtgc gggcgacctc cggggggcac caggtcctgg tctgcccctc ctgccaggag
     1321 gagcgaccgg attgggccgt ccagctcgac cggtgcgagt cctgcggcgc cacgcggctg
     1381 accgcgatgc tcggccaggt ggtgtgccgg gcgtgcgggc acgttcgcgg ggaaccggtc
     1441 gaacccgcat ggatgtcgac gggcgcttag ctcagaggta gagcactcgc cttacaagcg
     1501 agcggtcgga ggttcgagtc ctccagcgcc cacgagctca cgcgacacgg ggcggacgcc
     1561 ggtgtcgggt ggcgcgttcg aacgccgacg catgccggat caacgtcctc tcgctccacc
     1621 cccgacccat gaaggagagc ccgatcggaa ggtcgaacgc gaaccccatc gggaccgtga
     1681 cgttggggta gccggcgacc gccgccggtg tcgagttccc cccgacgtag tggtccccgt
     1741 tcgcgtagtc gatccgccac gccggtgcat tcgatggggc gacgagcgcg tccagccgat
     1801 gctcatccat caccgcgtcg accccctcgc cccgggacag gcggcgacag gtcgcgagcg
     1861 cctcgacgta ggccggctcg gtgagcggtc ccttctccgc ggccttctcg aaacgctcct
     1921 gcccgaagaa cggcatctcg acgtccgcgt gctcgtcgtt gaacgcgatg agttcggcga
     1981 gcgtccggaa cggctgtgac gggccgagcg atccgaggta cgcctccagg tcggtcttga
     2041 actcgaagag gaacacctcg agctcgggat cctcgagctc gtcggtgtgc gggatgtcgg
     2101 cggggtcgac gatctccgca ccgagatccc
//
LOCUS       DASUYD010000226        29724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1267037, whole genome shotgun sequence.
ACCESSION   DASUYD010000226 DASUYD010000000
VERSION     DASUYD010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 29724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29724
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267037"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..587)
                     /locus_tag="VF195_11900"
     CDS             complement(45..587)
                     /locus_tag="VF195_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581560.1"
                     /translation="MGGEVMPLGEARALGETVSAEAPYLAALYEEHVGRATALATLLT
                     GDASVAEDIAHDAFLRAAGRFRHLREPDAFGAYLRRTVVNACRARARRARRERLFLER
                     AWWVRKVEPDTSADDRDELLPLIKALPYRQRAALVLRYWDDLPEAQIADILRTSPRAV
                     NALLSRAMGTLRSGIGRSDR"
     gene            complement(706..1092)
                     /locus_tag="VF195_11905"
     CDS             complement(706..1092)
                     /locus_tag="VF195_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6581561.1"
                     /translation="MDVTEWMEGYRTAWETADTDLLLTLFTEDANYRSNPFEEPHVGH
                     EGIRAYWDGVTSQQRDARVSMGSPFVDGDRVAVEWWTRMISEGEEVTLPGCLLLRFGA
                     DGRCSDLREYWNFAPGLREPFQGWGD"
     gene            1170..1667
                     /locus_tag="VF195_11910"
     CDS             1170..1667
                     /locus_tag="VF195_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008237108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX6581562.1"
                     /translation="MDAAGVTVVVEIPGGSRNKFEMDHATGAIYLDRMLFTATRYPAD
                     YGFIEGTLGEDGDPLDALVLVGEPTFPGCRILARPVGVFGMRDEKGQDDKILCVPLRD
                     PASSHLVDLDDLRPSLLAEIEHFFAVYKDLEDKKVETQGFQDLGAALVIIDEARRRAT
                     ETNHR"
     gene            1859..3325
                     /locus_tag="VF195_11915"
     CDS             1859..3325
                     /locus_tag="VF195_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX6581563.1"
                     /translation="MTSWIRFVTSEASAAASGVRSALADVVWAPPPPVPDGSDIGLIV
                     FDRVSEDVLDLVRGSSRGGVRILAVGTSPEAVADIGPWPLLDAGASDVVVRMDDASLA
                     DEVSARMSRWAEVERLVHSSEVQDALAGRTLAWTSLLREIVEVARFSDASVLLIGESG
                     TGKDVVARLIHELDPRPFKDEFVTVDCRTFVGTHDGQDLAGHLGGSSEEADAPLFGDI
                     GLETLGVRDAGTIFLDEVGELPSSLRGSLLRNIEQRNRRPVGGTGTPMSNLRLICSTS
                     EEPSGPGSETADDFWERITTRFRLPPLREHRDDIPTIAEHLLRRLRPNGEEIQLDPAL
                     RAFLESRDYPANVHDLLTLIRGISARHAGPGVVTVGSIPQDERFELTTVDQSWQRQDL
                     ERAVRLAIDAGASLKTIGEAARDTAVRIALSYEGGDVARAAKRLHATTRTIASRTGVD
                     RAEADTVRSTGESSSIVEVPETALLVAADAGDEPVRKP"
     gene            complement(3721..4722)
                     /locus_tag="VF195_11920"
     CDS             complement(3721..4722)
                     /locus_tag="VF195_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX6581564.1"
                     /translation="MKRARISLLSLGGTIAMTGEKGERVVPTLASDALLAGLPQLDEI
                     ADVETDSVRQVPGAHLVQDDLLELARTIESRMAAGTDGVVVTQGTDTLEESAFALDLM
                     LGGEGPVVVTGALRNPSMIGSDGPANLLAAIRTAASPGARDLGVLVLMNDEIHSARFV
                     RKVHTSSPAAFASPLTGPIGWVTEGTVRIVARPTRPPGIGIPDRHPERPVALVRLWLG
                     DEGQLIRGVRDLGYEGLVVEGFGGGHVPAFIAETLGSLSSVMPVVLASRTGTGEILQR
                     TYGFAGSEIDLLERGLIPAGYLDGLKARILLSLLLRDGVARDDVPLEFRLRSTASLS"
     gene            complement(4953..5025)
                     /locus_tag="VF195_11925"
     tRNA            complement(4953..5025)
                     /locus_tag="VF195_11925"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4990..4992),aa:Arg,seq:acg)
     gene            complement(5198..5285)
                     /locus_tag="VF195_11930"
     tRNA            complement(5198..5285)
                     /locus_tag="VF195_11930"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5249..5251),aa:Ser,seq:gct)
     gene            5344..5949
                     /locus_tag="VF195_11935"
     CDS             5344..5949
                     /locus_tag="VF195_11935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6581565.1"
                     /translation="MPDVVVVGGGIVGAACAYELARAGASVTLLEREELAAGASGRNN
                     GLWVTPTDPQLLPMARASLARYLELADDSPVPYRLDRDPIGLLAVALDEYEMTLGARA
                     HDPYRAAAVEVDELGAEDALGLQPTLSPEVLGGWLVHHGHRLAPAALTIALALMAADR
                     GAAVRHHLPARALIEREGRVVGVVTDEGRIDADEVVVATGP"
     gene            5983..6276
                     /locus_tag="VF195_11940"
     CDS             5983..6276
                     /locus_tag="VF195_11940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581566.1"
                     /translation="MPIAGARGWLVRLDPGPDPLHRLVASAGWELATGRWEDGAVRAG
                     ALEDVSAVTSTLLHPATDGSLIAGSSRQPVLTPEAEEPGVPARIIRGAIRLVP"
     gene            6292..6513
                     /locus_tag="VF195_11945"
     CDS             6292..6513
                     /locus_tag="VF195_11945"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX6581567.1"
                     /translation="MRSGWWGIRPMTPDERPFVGRLADGLSVAAGHGSEGVILGAGTG
                     QLIASMLLGHHAPFDAKPFDPGRLGTVAG"
     gene            6525..7790
                     /gene="hemA"
                     /locus_tag="VF195_11950"
     CDS             6525..7790
                     /gene="hemA"
                     /locus_tag="VF195_11950"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX6581568.1"
                     /translation="MAVLALGISFRRAPIDLLERLAFTDDDLTKAYRRVQDVDEVEEA
                     VVLSTCNRVEMYGVVPSYHAGFLTLKRLLTETRGVAEAEIADGLYAHWERDAVEHLFS
                     VAGGLDSMVLGETQIQSQVREALRLAEGAAGSRMQALFHAASRAGRRIRQETSLGAAP
                     DAFVARAAALAEEVLGDLSGRSVVVLGAGTMASLGVKHLRRRGVGQIRILNRSLPHAR
                     ALAERNHAEPGDLDSLPDALRGADLVLSATGATGVVIHAEQVRAARSGHASPLVLLDL
                     AVPRDVESGAGALPGVTLFDADALRDRGSAEEDDAVARAREIVSQEVRRYIVRRRGDE
                     LSPVIRAIRHQGEAILRGELERQAARLADLSPDERAAVEALARGIVAKLLHDPIVKLK
                     ERSEPGSERRHAALLVDLLGLDPALLDGE"
     gene            7787..8692
                     /gene="hemC"
                     /locus_tag="VF195_11955"
     CDS             7787..8692
                     /gene="hemC"
                     /locus_tag="VF195_11955"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX6581569.1"
                     /translation="MTTLRIATRRSALALAQAGEVAALLSANAFRSEIVPMSTSGDEG
                     VASTESPSGMKGLWVDRILEALRTGEVDVAVHSAKDLPAEDEDDIVIAAVPKRADPLD
                     VLIGREATLPPGLVVGTSSVRRRAQLLAGFPGMGVTEIRGNVDTRLRKLADGEVDGLV
                     LAAAGLARLGIDPPHSRRLRVDEMVPAPGQGSLAVQVRAEDRTTRAVVSVLDHLPSRR
                     ALEAERALVQFLGGGCDLPLGALAAVKGDRIRMVGLVATTDGAKVIRTASESNEPGVL
                     ASTLAQRLRADGADRILAEVQGTPE"
     gene            8689..9450
                     /locus_tag="VF195_11960"
     CDS             8689..9450
                     /locus_tag="VF195_11960"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX6581570.1"
                     /translation="MSRSLAGRTIVVTRPAEQARPLVRELERRGARVLVAPTIRIVPA
                     RSAALTTALEKLAAGGFDWITLTSRATVDVLRDRLDSPRAVRARVAVIGDGTAAAFRR
                     WARRDPDLQPSTFTTTALARAFPRGTGHVLCARADIAPEGLETALEAKGWETERVDAY
                     RTVFATSLPRGARDAFRRGEVDAVTFTSASTVRGFVTARGQETARNPIGGPPKIVCIG
                     PVTAAAARDAGLRPKAVARPHTLGGLVDAVERALG"
     gene            complement(9447..10637)
                     /locus_tag="VF195_11965"
     CDS             complement(9447..10637)
                     /locus_tag="VF195_11965"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX6581571.1"
                     /translation="MKPKKRWDGYVSFSYLEAGVDYKEFALSPQIGRVEPFVYPLTDE
                     QNERADRLLHGCVCISLHDHCGITPVDMNENDEYVREGREWYGYEGLAASGLDAVFEN
                     FLDGTATITSKSGWKWTDVIHDLGMHLADVAHQSTVFVGGTVDDIEEAHRSGRIAMVP
                     CMEGAAMIENELDRLDVLFGLGVRMCGVAYSESNQLGGGVKDPGDGGLTRFGLDAVRR
                     MNRLGMAIDVSHTGDVTAMQTCEVSERPVFLSHSGARALFSERKLKHDDLLKAVAETG
                     GVIGIEGSPHTTISPAHPRHSLEGVMDHFEYCVDVMGIDHVGFGPDTFFGDHVGLHGM
                     YAAAFDTGEVPEYEPVEYVEGLENPAEFPNVIRWLVGHDYTDEQIAKIVGGNVLRALR
                     EVWV"
     gene            10679..11659
                     /gene="hemB"
                     /locus_tag="VF195_11970"
     CDS             10679..11659
                     /gene="hemB"
                     /locus_tag="VF195_11970"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015105406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX6581572.1"
                     /translation="MSFPHSRPRRMRRTPALRSLIRENDVRPGDLIAPLFVKEELTEP
                     APIASMPGQSQHTLESLRKEAVEVGERGVRAFILFAVPARKDPEGSEAWNPEGIGQQA
                     IAALRAEFGDDAVVIGDLCLDEYTDHGHCGVLNERGAVVNDATVELYQRIAIAQAEAG
                     AHLVGPSGMMDGQVAAIRSALDASENQDIGILAYAAKFASALYGPFREAAEGAPRFGD
                     RTGYQQDPANLDEALREIRTDIDEGADIVMVKPALPYLDVIRRAKDETRFPMAAYHVS
                     GEYAMVKAAAANGWLDERRVVLELLTSIRRAGADLILTYHAKDAADWLRG"
     gene            11667..12209
                     /locus_tag="VF195_11975"
     CDS             11667..12209
                     /locus_tag="VF195_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6581573.1"
                     /translation="MARYVALLRGINVGGKNAVPMPALKASFEDAGFGNVSTYIQSGN
                     VVFDTPSSSQEELTRKVEGIIRKAFAHYDASVVLRSRSQMRAVVQKAPKGFGTEPAKY
                     RYDVVFLKPPLTAKSAIKNIATKEGVDRIWAGTGVLYMSRLDSQATQSRLNRVASLPI
                     YKHMTIRNWNTTTKLAELVS"
     gene            complement(12216..13139)
                     /locus_tag="VF195_11980"
     CDS             complement(12216..13139)
                     /locus_tag="VF195_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX6581574.1"
                     /translation="MTVGSDDVRAAAERIAGRIRRTPVLELGELRGASVVAKLELLQY
                     TGSFKPRGAFNKLLASEVPPAGVIAASGGNFGLAVAYAAREAGVPAEIFIPSSSPRTK
                     IDRVRAQGAAVTIIEGYFAEANEALGARAVETGALLMHPFDQPEVIAGQGTLGVEIEE
                     QVLHVDTVMIGVGGGGPLAGIAAWCGDRVRVVGVEPEGCASMTAALAAGEPVTVPVGG
                     LASDSLGAARVGDHTFPIVRDRVERMVLVTEEQIREAQRALWEEVRLIVEPGGAAAFA
                     ALRSGAYPVSPGERVCVVLSGANCDPSTVTA"
     gene            13179..14453
                     /gene="hemL"
                     /locus_tag="VF195_11985"
     CDS             13179..14453
                     /gene="hemL"
                     /locus_tag="VF195_11985"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012638974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX6581575.1"
                     /translation="MNGSDELFARAMLAIPGGVNSPVRAFGAVGGVPRFALRGEGAYL
                     EDADGARYVDLCQSWGASIFGHARKEIVDAAAEAASRGTSFGIPTEAEVLLAERIVSM
                     VPSIEQVRLVSSGTEAAMSAIRLARGATGKDAVIKLEGCYHGHSDALLAHGGGSGLAT
                     FGIPSTPGVTAGATRDTLVAPFNDLEAVRALLDANEGSVAALIVEPVPANMGVVPPDD
                     GYLDGLRQLCDEHGALLIFDEVITGFRIARGGAQERFGVSPDLTVLGKVMGGGFPCAA
                     FGGRRDLTEQLAPAGHVYQAGTLSGNPVAVAAGLAALDLIDAEDPYPGLETAAEDLQA
                     GLAGAFEANGIGVTINRAGSLFSVFFSEGPIRNFDDAKAADHERFARFFHHMLAEGVY
                     LPPSGYELWTLCSAFGEAEEEAVLGAAGRFRD"
     gene            complement(14466..14909)
                     /locus_tag="VF195_11990"
     CDS             complement(14466..14909)
                     /locus_tag="VF195_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581576.1"
                     /translation="MRQWELIGITDTVDWSPPRRDPVPSGDDGDHYPAVLWTTDGSLR
                     LRGVSWVASQHLGIHPRFLDGRELLEAFGMEGESLPILEAHAAALTGETVTFSLPGME
                     GRMWCRVAPTHDAGEHIVGTFCIATEPASDGDELETVQDTTLTLV"
     gene            15104..15619
                     /gene="msrA"
                     /locus_tag="VF195_11995"
     CDS             15104..15619
                     /gene="msrA"
                     /locus_tag="VF195_11995"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX6581577.1"
                     /translation="MPTEIATFGAGCFWGVEHLFRGIPGVTEAISGYSGGFVENPSYQ
                     LVCTDTTGHAEVVQVTFDPELVSYDQLLEVFWAMHDPTQLNRQGPDRGSQYRSIVLAH
                     SDEQRAAAEVSRDRVQPRYERPIATEIHPFVAFYPAEDYHQRYYLNNGHEPYCHSIPA
                     GLLEELGLVRT"
     gene            complement(15616..16200)
                     /locus_tag="VF195_12000"
     CDS             complement(15616..16200)
                     /locus_tag="VF195_12000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581578.1"
                     /translation="MTAKRLELPGGPPELAPPDRGDEVRQLVPEPREDLSEHYVLLRG
                     IRPLPGERDALQDPARVEQRSDRRTWLELLQSPLAPEEELALGPVGEDEEHEAREPEV
                     LEEEGRPEAPDEPGTIADERLVRVVLRDHAEVDVGVHVRLSTPTRAAEERGHDPVVLL
                     ACRDEPVQERLEIVRPRRRLVHAVELGRVSPRRR"
     gene            complement(16469..17137)
                     /locus_tag="VF195_12005"
     CDS             complement(16469..17137)
                     /locus_tag="VF195_12005"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HEX6581579.1"
                     /translation="MSDPEIRIEENGPYEVKGPLPIARTRTVKTEYGEPVDYAPFEPL
                     EADETYRLCRCGKSGNKPFCDDSHLEEPRVDVTERADHGPRDDRTDVFQARDGAIISD
                     DLSICTKAGYCKDRFTGVWQMLGDPDPAVRERMGRMVQLCPSGRLAWADSVDSPDHEP
                     EYEPEIGVFRNGPLFVRGGVRVVGADGEAYEVRNRQTLCRCGYSQNKPFCDGSHRLVG
                     FRDG"
     gene            17200..17571
                     /locus_tag="VF195_12010"
     CDS             17200..17571
                     /locus_tag="VF195_12010"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6581580.1"
                     /translation="MSAGTIDEYAEALGDWRGEVVGELDALIRRAAPKATGSIKWAQH
                     VYEQDGPFAYVKAFTSAVNFGFWRGADLTDTEGRLEGTGDRMRHVKIRTPEDFDKARS
                     TAWVKEAVEFNAEKGDPTKAR"
     gene            17620..19656
                     /locus_tag="VF195_12015"
     CDS             17620..19656
                     /locus_tag="VF195_12015"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEX6581581.1"
                     /translation="MFEELNPEQGAAVRHGEGPLLVVAGAGSGKTMTLAARVASLIER
                     GTRPERILLLTFSRRGAREMLSRAERLTAHDDAGRVWGGTFHAVGNRLLRLHGRALGL
                     APDFTVLDQADGADVMNLLRDELGYSSRERRFPRKDTLAAIYSRTVNAGERLVDVLKG
                     HYPWCLDEAEGIVDIFRAYTERKRRQHVLDYDDLLLFWKALATSPATGAQLAAMFDHV
                     LVDEYQDTNALQADILEGMRPSEGPRNLTVVGDDAQAIYGFRAATVKNILEFPERFPG
                     TTVIKLERNYRSTPPILAASNAVIALSPQRHEKTLRSSRESELRPVLRACLDEAEQSD
                     AVCRAVLGYREEGVPLKAQAVLFRAAHHSDHLEVELARRNIPFVKYGGLKFMEAAHVK
                     DTLACLRVLENPFDEVSWFRVLQLLEGMGPASARRLMDDLEVRRTDDADATSPLARFL
                     AQPLAVPKGAAQGIRDLRSVLGDCLEAETVPPAAQIARLRTFLKPVIARKYDAAAART
                     RDLEQLELLATESASRGQFLADLTLDPPSSTGDLAGPPHLDEDHLILSTIHSAKGLEW
                     DVVHVIHAADGMIPSDMSTGDDDEIEEERRLLYVALTRARDALHITYPQRYYRRPRGL
                     EDPHSYSQRSRFLPDEVLLHFEVIGPHVLSVADLSEPVTAAADVDAFLAGLWTD"
     gene            19732..20181
                     /locus_tag="VF195_12020"
     CDS             19732..20181
                     /locus_tag="VF195_12020"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX6581582.1"
                     /translation="MHRSRRIAAVLVSIGLATLAFTLPASAGGRPFTTSLSGAAERPT
                     AGDPDATGTARVTVNPGTREVCWSVSVDNVDLPVIAAHIHIGGADTFGPPVIFLLPEG
                     VSDADGEFSGCSTIDRSLAFALLLSPEDYYVNVHTDLFQAGAARGQL"
     gene            20248..20895
                     /locus_tag="VF195_12025"
     CDS             20248..20895
                     /locus_tag="VF195_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6581583.1"
                     /translation="MEGALLDLYDTVARTRWRELSDRISGELGVDVAALYRAYGLTRA
                     ERSIGAFDGPEGDMAAIVEAAGSESTPELIDSLVAMERQFTETGVELWEDSLPVVYEL
                     RQRGIKTALVSNCSHSTRGIVDRLGLVDAFDEVVLSFEVGLRKPDPAIYREALGRLDL
                     EPGGAVFVDDQQEYCDGAAAIGIETYLIDRSGDATPDENGHRVIRDLRALLAVSA"
     gene            complement(20878..21735)
                     /locus_tag="VF195_12030"
     CDS             complement(20878..21735)
                     /locus_tag="VF195_12030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581584.1"
                     /translation="MKKLLLSLTTLCMLASCAEGGTPVSPRDAVLDAMTAVYEAGTYH
                     QELRMSMSAEGETFSITADADVDSATRQVAMTMDLGMMGGEVEMVMDDGVIYMRSPVF
                     EGAPTPWVSFDPSKMDPATAARFGGFGAGTTDPSAYAGLFAGIFDVKASGETDIDGIA
                     TTRYTGTIDLQKVLEGFAEIVGDDADAETTEQLEAAVEQFEALGIDGDIPFQIWIDDD
                     GLPRRQRITMDFGDLVPGAGDAQMEMTVDYSAFAEPVNVKRPKATEVTDITEVMNEGA
                     AGEKSGAYG"
     gene            complement(21789..22466)
                     /gene="hemQ"
                     /locus_tag="VF195_12035"
     CDS             complement(21789..22466)
                     /gene="hemQ"
                     /locus_tag="VF195_12035"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013474527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HEX6581585.1"
                     /translation="MTETIYALYPVFAASQELRAELFEDQALRNAGDEIESLFKDWAG
                     RVDVRGSYSTVGFRADADLVLWLTAAGTEDLQRFLVEFRRTRAGDLLDPVVTLMGLVK
                     PAEFTPDHQPAFVRGEAPRTYLCVYPFVRSAEWYLLPPQERGELLAEHGVLGREFPQV
                     LANTTSAFGLGDWEWILAFETDELDALVDCIRRLRDSKTRLYTKVETPFFTGIRKSIA
                     DVLDDLR"
     gene            complement(22463..23830)
                     /gene="hemG"
                     /locus_tag="VF195_12040"
     CDS             complement(22463..23830)
                     /gene="hemG"
                     /locus_tag="VF195_12040"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEX6581586.1"
                     /translation="MNPRVAVVGGGIAGLATAFRLQRAGVEPVVLERSSTYGGIIVPP
                     VPVGDLALEPGPDSLAARKPWGSALCRDLGLTLVPPGTTGAFLWTESGLVPYLRGAPF
                     GIPGDVGDVLRWPGVSSRGRVRALADLVKRTRRHGAEETLGELLRRRFGDEATERAIA
                     PLLAGLHAGDVDRLSAPATFPELVAWEESQGSLIRGAQAAQRAAGRADPGPLFLRPRS
                     GMRELVDVLAATVGERLRTGVEVTGVTARTVDLADGSSEHVDAVVLACGAPVSASLLG
                     PSAPAGLSTIGSVSTGVVFLVYPAGTAGALPDGTGFVVPRGAAPMTAATFMSRKWPNP
                     SFGDRAVVRCYVGGAGDEDVLDAPDDDIVGACVRHLSAALDLPAPVAARVHRWWAAMP
                     QYERGHGALVEEIREGLPPGIFLVGSAFDGVGVSDLARAAEETADQVLAHAGAPRPGR
                     RKEPA"
     gene            complement(23827..24795)
                     /gene="hemH"
                     /locus_tag="VF195_12045"
     CDS             complement(23827..24795)
                     /gene="hemH"
                     /locus_tag="VF195_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006446402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX6581587.1"
                     /translation="MTAPTGVLVMAYGTAGGPEDIERYYTDIRGGRVPSPEHLADLRA
                     RYEAIGNVFPLLDTTRVQAEGLVARLSEDGTPFRAFLGMKHSPPFIPDAVAQMRGEGV
                     ERAVGIVMAPHWSGMSVETYVERVLAAVADQGGGPVFSFVREYGAHPSFVRFLTARVA
                     DALEDVPPAERSATRVLFTAHSLPTRVVEDGSLRCKTCMCDGSCRYRAGLQETADLVA
                     GTLGLESYGIAWQSAGRTSDPWWGPPVEESILQAAAMGHPAVVVCSAGFVADHLETLY
                     DLDVEAKALAEGAGIRFARTEMPNDDPAFLEVLADVVRDHLVTAAP"
     gene            complement(24792..25838)
                     /gene="hemE"
                     /locus_tag="VF195_12050"
     CDS             complement(24792..25838)
                     /gene="hemE"
                     /locus_tag="VF195_12050"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX6581588.1"
                     /translation="MTGLPPNDRLLLALRRRPVDRTPVWFMRQAGRSLPEYRARRGDA
                     DILETCRRPDEVVELTLQPLRRMPLDAAILFSDIMVPLHATGVPIRIEAGRGPLIDPP
                     LRDASGIERLRPLDPETDEPYVAEAVRLLVKELEVPLIGFAGAPFTLASYLIEGGPSR
                     TFSRTKALLFTEPAVFDRLLRMLADIVVAHLRAQVDAGVHAVQLFDSWAGALDRDDYR
                     GMVMPHTARVFEAIAGMGVPAIHFGVGTSHLLDLMRDAGGDAIGVDHRLPLDDAWAVI
                     GHDRAIQGNLDPAVLLGYWDAVERKAEDVLARAALRDGHVFNLGHGVLPDTPVEHLQR
                     LVDLVHAETERPAP"
     gene            complement(25844..27433)
                     /locus_tag="VF195_12055"
     CDS             complement(25844..27433)
                     /locus_tag="VF195_12055"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX6581589.1"
                     /translation="MTAGETVTEHIKQEARVAGLRDFRTPSLEAVEKRRLQLWILTAT
                     LLVSISLGVVFVSTWQTSATRAFAATPVMRIAVVLLSIAFCIYAIDKERHLRTLSRLL
                     IDERVLTTALSNRLREVELLLGAGKAMNDVLELSVVLDTMLRSAVELLDSDSGSVMLL
                     EGAHLVVACSHGNEHADGRSVQLGEGIAGHVASTREALLVQGKADPGDFPGLVPHSLP
                     VSSAISAPLIHREEVLGVLNVNSAGDRLFSEFDLRAVSLFAEQAAAAIANARLYETER
                     AQVEELTHLNRLKSEFVALVSHELRTPIASIIGAVSSARKPELHEHRGELEDIVERAA
                     LRLGTMVEDLLAAAELERGEAVNRLEPVDLAKVVREAAADHRVLDRDLQVDASQPAIA
                     VCDREYLRRILDNLIDNAFKYGRPPVRIKVTEDSGHVGFAVLDSGRGVAAEDRARVFN
                     RFERLDSMSGHGLGLGLSVVQGLVEAMGGDVRIEEAPGGGAAFRILFPRPKRSMLEDA
                     LMDTAGPPAAPTPPVQASSVS"
     gene            27658..28005
                     /locus_tag="VF195_12060"
     CDS             27658..28005
                     /locus_tag="VF195_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013226217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX6581590.1"
                     /translation="MVTVDQVRAFALTLPRTTEALVRDRVKFRVGRIVYLAFSRDETT
                     MGFAFPKEEREALVRSEPERFLMPDRSDLRFDWVVVRLDSIDAAEMREIVLDAWRMVV
                     PKRVAAAYEDSVG"
     gene            28463..29680
                     /locus_tag="VF195_12065"
     CDS             28463..29680
                     /locus_tag="VF195_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581591.1"
                     /translation="MKRLIVVSLALTTALMMFGGMAPAQAAVTQAVDDDGMASLTSCN
                     AVTPTHITIQAGVNAAAAGDTVKVCPGTYTENVTVNKALTLSGAKAGVNGTRVVNPAN
                     ESIVHPSDPNQPTFMLEVSAITVNGFLIEGNTNNAGIQTRPTASGYQILNNIVRDNVF
                     GIYLHGDGATTTLVRRNLLDNNNRPGAASGNGIYSDQGALGITIQANSFRNHTNTGIV
                     FANAAVVQESIIVQNNRSLNDNTFVALFDVQNATVVANQTNDTVDADDFGSTIFVGGD
                     SAGIVIQRNVLTNPGFSGIAVRDVLGTAGSTANVNVLGNTVTGAEGSGIDVTAEDFAA
                     VSARNNTLRTNGIDGIFFGSSTVGNQIRSNRASGNVNLDCEDDSTGSGTAGTANTWAN
                     NDGVTDDPNGICP"
BASE COUNT         4769 a        10355 c        10082 g         4518 t
ORIGIN      
        1 ggcatcgcct gagagcgcct tcacgacctg tcgttcgagt tcgttcaccg atcgctcctt
       61 ccgatcccgc ttcgcagcgt ccccatcgcc ctcgagagga gggcattcac cgctcgcgga
      121 gaggtgcgca ggatgtcggc gatctgcgct tcgggcaagt catcccaata ccggaggacg
      181 agggctgcgc gctgtcgata gggcaacgcc ttgatgagcg gcaggagctc gtcgcggtca
      241 tccgcagagg tatccggctc gaccttgcgg acccaccacg ctcgttcgag gaacagacgc
      301 tcccgccggg cacgacgggc ccgcgcacga cacgcgttca cgacggtcct gcggagatag
      361 gccccgaacg cgtccggctc ccgaaggtgc cggaagcgcc ccgctgcccg aaggaaggcg
      421 tcgtgcgcga tgtcctccgc gacggacgcg tctcccgtca agagggtcgc aagggcggtc
      481 gctcgaccca catgctcctc gtagagcgcg gcgagatagg gagcctcggc ggacacggtc
      541 tcccccagag ccctcgcctc gcccagtggc atcacttcgc ctcccatccg aacctactga
      601 tagatgccca ggctgggagt tccgcttcgg tgatcagcga acatttctcg gtcaaccacc
      661 aggacccgac ctgagcgcga tcgccgagta cttccgccgg tccggctagt ccccccagcc
      721 ctggaagggc tcgcggagtc ccggggcgaa gttccagtac tcccgcaggt cgctgcatcg
      781 cccgtcggca ccgaaccgga gcaggaggca tccagggagc gtgacctcct caccctcgga
      841 gatcatcctc gtccaccact cgaccgcgac gcggtcaccg tccacgaagg gactgcccat
      901 cgagacccgg gcgtcgcgct gctggctcgt cacaccgtcc cagtacgcgc ggatcccctc
      961 gtgtccgaca tgcggctcct cgaacgggtt cgaccggtag ttggcgtcct cggtgaagag
     1021 ggtgaggagg aggtccgtgt cagccgtctc ccacgccgtc cggtatccct ccatccactc
     1081 cgtgacgtcc atcgcccgag cgtatcgggc ggtgtgtcgc atgaccggcc ggtcgcggcc
     1141 ggatctcggg aggcgactat ggttcggaca tggacgcggc aggcgtgacg gtggtcgtgg
     1201 agatcccagg tggatcccgg aacaagttcg agatggacca cgcgaccgga gccatctacc
     1261 tcgaccggat gctcttcacg gcgacccgat acccggccga ttacggattc atcgagggga
     1321 cgttgggaga ggatggcgat cctctcgatg ccctcgttct cgtcggagag cccaccttcc
     1381 ctggatgccg gatcctcgcg cgaccggtcg gagtcttcgg gatgagggac gagaaggggc
     1441 aggatgacaa gatcctgtgc gttccccttc gtgacccggc gtcgtctcac ctcgtggacc
     1501 tcgacgacct ccgcccctcc ctgctcgccg agatcgaaca cttcttcgcc gtctacaagg
     1561 acctggaaga caagaaggtg gagacgcagg gcttccagga tctcggggcc gcgctggtga
     1621 tcatcgacga ggctcgacgg agagcgacgg agacgaacca tcggtgagga tgtgatctga
     1681 gagtcacgcg gggtggctcg atgaccatcc cccgatgggg ggacaccacg tgacgatcgg
     1741 ccacgtcgcg tcggcctatc cgcctaggca ggtgcggcga cttgtccgtc gcccatggct
     1801 gcgcgaccct tccctcggac atccggaacc cacccggagg gggcgtgctt cgcgaccgat
     1861 gacgagctgg atccgtttcg tgaccagtga ggcctcagca gcggcatccg gcgtgcgatc
     1921 cgcgctggcc gacgtggtgt gggctccgcc gccaccggtc ccggatgggt cggacatcgg
     1981 attgatcgta ttcgatcggg tctccgaaga cgttctggat ctcgtacggg gatccagccg
     2041 aggcggggtc cgcatcctcg ccgtcgggac ctccccggag gcggtcgcag acatcggccc
     2101 ctggccgctc ctcgatgccg gggcctcgga tgtcgtggtc cggatggacg acgcaagtct
     2161 ggccgacgaa gtctcggcgc gcatgagtcg ctgggcggag gtggaaagac tcgtccactc
     2221 gtcggaggtg caggacgcgc tcgcgggacg gacactcgcg tggacctccc tcctgcgcga
     2281 gatcgtcgag gtcgcgcgct tcagcgacgc ctccgtcctc ctgatcggcg aaagcggtac
     2341 cggcaaggac gtcgtcgcgc ggttgatcca cgaactcgac cctcgtccgt tcaaggatga
     2401 gttcgtgacg gtcgactgca gaaccttcgt ggggacacac gacggacagg atctcgccgg
     2461 gcacctgggg ggctcgtcgg aggaagcgga tgcccccctc ttcggcgata tcggtctcga
     2521 gacgctcggt gtccgggatg ccgggaccat cttcctcgac gaggtcgggg agctcccctc
     2581 atcgcttcgg ggatcattgc tccggaacat cgagcaacgg aatcggcggc ccgtcggcgg
     2641 aacaggcacg ccgatgtcca acctgcgact gatctgctcg acgagcgagg agccgtcggg
     2701 accgggctcc gagacggccg acgacttctg ggagcggatc accacccggt tccgcctccc
     2761 tcccctccgc gagcaccgag acgacatccc gacgatcgcc gaacacctcc ttcgccggct
     2821 gcgacccaac ggagaggaga tccagctcga cccggcgctc cgtgccttcc tggagagccg
     2881 ggactatccc gcgaacgtgc acgacctcct gaccctcatc aggggcatca gcgcccgaca
     2941 cgccggaccg ggcgtcgtca cggtcggatc gatcccccag gatgagcgct tcgaactgac
     3001 gacggtcgac cagagttggc agaggcagga cctcgaacgt gccgtccgcc tcgccatcga
     3061 tgcgggcgct tccctcaaga ccatcggcga ggcggcgcga gatacggccg ttcggatcgc
     3121 gctgtcgtac gaaggaggag atgtcgcgcg cgccgccaag aggctccacg cgacgacccg
     3181 gacgatcgca tcacggacgg gcgtcgatcg agcggaggcc gacaccgtcc ggagcaccgg
     3241 cgagtcctcg tcgatcgtcg aggttcccga gacggcgtta ctcgtcgccg ccgatgccgg
     3301 cgatgaaccc gtgcggaaac cgtaaccgcg gaggcccgcg accgacttct ctgggtgggc
     3361 aacccgtcga tgcttcgagg atggcctgag aacgacgaga ggtggcccct tgagctgagc
     3421 gctgtgcgcc gagagatcac ccgtccgttc gtcccggcga agggcctagg tgccctccag
     3481 gtacaaggtc tgcttgcggg aaccggacga accgctcggc cggccccgca tccttgcccg
     3541 ggccggggag ttgttcgtgc acgcgcaacg aacgccgatc gagttgggac gattcccacg
     3601 tacgccttct cgccttcgcg ggagccatga cgaggtcccc atctcgctcg gctcccccac
     3661 actcgccgga tgagtggccc gtacgcgctc ctcgagggtg cgctgccgtc gaggtcggga
     3721 tcaggagagg gatgcggtcg agcggagccg gaactccaag ggaacgtcgt cccgcgctac
     3781 tccatcgcgg aggaggaggg acagcaggat gcgagccttg aggccgtcga ggtacccggc
     3841 cgggatgagc ccccgctcga ggaggtcgat ctcggacccg gcgaacccgt acgtgcgctg
     3901 gaggatctcg cccgttcccg tgcgggacgc gagcacgacc ggcatcaccg atgagaggga
     3961 cccgagcgtt tcggcgatga aggccggcac gtggcctccg ccgaacccct cgaccacgag
     4021 tccctcatag cccaggtccc gaaccccccg gatgagctga ccctcgtcgc cgagccacag
     4081 cctgacgagc gccaccgggc gctccggatg gcggtcgggg atcccgatgc cgggggggcg
     4141 agtcggccgg gccacgatcc ggacggttcc ctcggtcacc catccgatag gtccggtcag
     4201 cggcgaggcg aacgcggctg ggctcgaggt gtggaccttc cgaacgaatc gagcggagtg
     4261 gatctcgtcg ttcatcaaga ccaacacgcc caagtctcgc gccccaggac tcgccgcggt
     4321 tcggatcgcc gcgagcaagt tcgcgggtcc atccgagccg atcatgctcg ggttccgaag
     4381 cgcgcccgtc acgacgactg gcccctcccc tcccagcatc aagtcgagag cgaaggcgct
     4441 ctcctcgagc gtgtctgtcc cctgggtgac gacgacaccg tccgttcctg ccgccatccg
     4501 cgactcgatc gtcctcgcga gctcgagaag gtcgtcctgg acaagatgcg cccctggcac
     4561 ctgacggacg gaatcggttt cgacgtcggc gatctcgtcg agctgcggca gcccggccag
     4621 gagggcatcg ctcgccaggg tcggcacgac ccgctcgcct ttctcgccgg tcatggcgat
     4681 cgtcccaccc agcgacagca ggctgatcct cgctcgcttc atgacccggc gacctccatg
     4741 gatgactcgg cgtttcgggg cgcgtgctca gagacccaac ataaccgtga ctgatgcaag
     4801 ctcgacgcgc tcctgcgtga gccgaacagc actccgtcga gaccgaccgc atcagcgagc
     4861 cggcaagtct gagagcgaca cggcgtgcat ggcgttcgag ctcaccggat agctaacctc
     4921 cgggcggtca cacagccgct gacctgcgga agcgcgccgg gagggagtcg aacccccaac
     4981 cttctgatcc gtagtcagat gctctgtcca ttgagctacc ggcgccgaga ggcaactcta
     5041 ccagtggccg atttcgcgcc tccggaaagg cggtccgacc ccaaacacga tgcgccgaag
     5101 cgtttccatg ctggcatctg cacggacgat gacgatcgga gcctcggcct gaacacagcc
     5161 gaaggcacgc gacccgctcc gcggagacgg gagtacgcgg agacgggagg attcgaacct
     5221 ccgagggagt gttaccccct aaccgcttag caggcggctg ctttcggcca ctcagccacg
     5281 tctccacggc cgagtctatc gaggacccgg ttctcaccgg acgggggtag gctccccggc
     5341 ggcatgccgg acgtcgtcgt cgtaggcgga gggatcgtcg gtgcggcgtg cgcatacgag
     5401 ctggcgcgag ccggcgcatc ggtcacgctc ctcgagcggg aggagctggc tgcgggagcc
     5461 tctggccgca acaacggcct gtgggtcaca cccaccgacc cacagctgct cccgatggcc
     5521 cgggcgagcc tcgcgcgcta cctcgagctc gccgacgaca gcccggtccc gtaccggctc
     5581 gaccgagatc cgatcgggct gctcgcggtc gccctcgacg agtacgagat gaccctcgga
     5641 gcgcgcgccc acgatcccta ccgcgcggcg gccgtggagg ttgatgagct cggagcggaa
     5701 gacgccctcg gcctccaacc cacgctctca cccgaggtcc tcggcggctg gctcgtccac
     5761 cacggccacc ggctcgcgcc cgcggcgctc acgatcgcgc tcgcgttgat ggcggccgac
     5821 cgaggagcgg cggtccgaca tcatcttccg gctcgggctc tgatcgagcg cgagggacgc
     5881 gtggtcggcg tcgtcacgga cgagggccgg atcgacgccg acgaggtggt cgtcgccacc
     5941 ggcccgtgaa cacacgcgtt gctggatccc gcaggtcatc ggttgccgat cgcgggagcc
     6001 cgcggctggc tcgtccggct cgacccgggt cccgacccgc tccatcgcct cgtggcctcg
     6061 gcggggtggg agctggccac cgggcggtgg gaggacggcg ccgtgcgtgc gggcgccctg
     6121 gaggatgtgt cagcggtgac ctcgacgctc ctgcatccag cgacagacgg atccctgatc
     6181 gcgggttcgt cccggcagcc ggtgctcaca cccgaggccg aggagcccgg cgttcccgcg
     6241 aggatcatcc gtggggcgat ccggctagtg ccgtagctcg ccgaggcgca cgtgcggtcc
     6301 ggctggtggg ggatcaggcc catgaccccg gacgagcgtc cgttcgtcgg caggctcgcc
     6361 gacggtctgt ccgtcgccgc cggacacggc tcggagggcg tgatcctcgg cgccggcacg
     6421 gggcagctga tcgcgtccat gctgctcggg caccacgccc cgttcgacgc gaagccgttc
     6481 gaccccggtc gtctcgggac cgtcgctggg taggattcct cgtcatggcc gtcctcgccc
     6541 tcgggatctc gttccggcgc gcgccgatcg acctactcga gcggctcgcg ttcaccgacg
     6601 acgacctgac caaggcgtac cggcgcgtcc aggacgtgga cgaggtagag gaggccgtgg
     6661 tcctgtccac gtgcaaccgc gtcgagatgt acggggtggt ccccagctac cacgcgggtt
     6721 tcctgacgct gaagcgactg ctcacggaga cgcgaggggt cgcggaggcc gagatcgcgg
     6781 acggcctcta cgcgcactgg gaacgtgacg ccgtcgagca cctgttctcg gtggccggag
     6841 gactcgattc gatggtgctc ggcgagacgc agatccagag ccaggtccgg gaggcgcttc
     6901 gcctcgctga aggcgcggcg ggttcccgga tgcaggcctt gttccacgcg gcgtcgaggg
     6961 ccggacggcg gatccgccag gagacgtcgc tcggcgccgc gccggacgcg ttcgttgcgc
     7021 gcgccgccgc gctcgcggaa gaggtgctcg gcgacctctc cgggcgatcc gtcgtggtgc
     7081 tcggcgccgg cacgatggcc tccctcggcg tgaaacacct ccgcagacgc ggcgtggggc
     7141 agatccgcat cctcaaccgc tcgctcccgc acgctcgcgc gctcgccgag cggaatcatg
     7201 cggagccggg cgatctcgac tcgctccccg acgccttgcg aggggcggac ctcgtcctct
     7261 cggccaccgg agcgaccggc gtggtgatcc acgcggaaca ggtccgtgcg gctcggtcgg
     7321 gtcatgcgtc cccgctcgtc ctgctcgacc tggccgtgcc gcgcgacgtg gagtcggggg
     7381 ccggcgcgct ccccggcgtg acgctcttcg acgccgacgc gctgcgcgac cgtggttccg
     7441 cggaggagga cgacgcggtg gcgcgcgcca gggagatcgt gtcccaagag gttcggcgat
     7501 acatcgtccg tcgccgcggc gatgagctct cccccgtgat ccgtgcgatc cgccatcagg
     7561 gtgaggcgat cctgcggggc gagctggagc gccaggcggc ccgtctggca gatctttccc
     7621 ccgacgagcg cgcagccgtc gaagctctcg cgcgcgggat cgtcgcgaag ctcctccatg
     7681 acccgatcgt gaaattgaag gaacggtccg aacccgggtc ggagcgcaga cacgcggcct
     7741 tgctcgtgga tctcctcggc ctcgaccccg ccctcctcga cggggagtga ccaccctccg
     7801 gatcgcgacg cgacgctccg ccctggcgct ggctcaggcg ggagaggtgg ccgcgttgct
     7861 gtccgcgaac gccttccgat cggagatcgt cccgatgtcg acgtcgggcg acgaaggggt
     7921 cgcatccacg gagtccccgt ccgggatgaa gggtctctgg gtcgaccgga tcctggaggc
     7981 gctccgcacg ggcgaggtgg acgtcgcggt ccattccgcg aaggacctgc ctgccgagga
     8041 cgaggacgat atcgtgatcg ccgctgtccc gaagcgcgcc gaccccctcg acgtcctcat
     8101 cggccgcgag gccaccctcc cgcccgggct cgtggtcggc acctccagcg tccggcgtcg
     8161 cgcccagctg ctcgccggct tccccggcat gggggtcacc gagatccgcg ggaacgtcga
     8221 cacccggctc cggaagctcg ccgacggtga ggtggacgga ctcgtgctcg cggcagcggg
     8281 tctcgcgcgt ctcgggatcg acccgccgca ctcccggcgt ctccgtgtgg acgagatggt
     8341 gccggcaccc ggccaaggga gcctcgccgt gcaggtgcgg gccgaggacc ggaccacgag
     8401 ggccgtcgtc tccgtgctcg atcacctgcc ctcgcggcgc gcgctggagg cggaacgggc
     8461 gctcgtccaa ttcctcggcg ggggatgcga cctcccgctc ggggcgctcg ccgcggtgaa
     8521 gggagaccgg atccgcatgg tcggtctcgt cgcgaccacc gacggcgcga aggtgatccg
     8581 aaccgcgtcc gagagcaacg agcccggcgt gctcgcctcg acgctcgcgc agcgcctgcg
     8641 cgccgacggg gccgaccgga tcctcgcgga ggtgcagggg acaccggagt gagccgctct
     8701 ctcgccggac ggacgatcgt cgtcacgcgt ccggccgagc aggcccgtcc gttggtgcgg
     8761 gagctggagc gccgcggagc gcgcgtcctc gtcgcgccca cgatccggat cgtccccgcg
     8821 cgctccgcag ccctgaccac agccttggaa aagctcgccg ccgggggatt cgactggatc
     8881 acgctcacga gtcgggcgac cgtcgatgtg ctgcgggatc gactggattc gccgcgcgcc
     8941 gtccgcgcga gggtcgcggt gatcggggac ggcaccgcgg ctgcgttccg tcgctgggcc
     9001 cggcgtgatc ccgacctgca gccgtcgacg ttcaccacca cggcgctcgc gcgtgccttc
     9061 cctcggggta ccgggcatgt cttgtgcgct cgcgccgaca tcgccccgga aggactggaa
     9121 acggcgctcg aggcgaaggg ctgggagacc gaacgggtgg acgcctaccg gacggtcttc
     9181 gccacctctc tcccccgcgg tgcgcgcgac gcgttccgcc gcggggaggt cgacgccgtc
     9241 acgttcacga gcgcctccac ggtccggggc ttcgtgaccg cacgcggcca agagacagcg
     9301 cgaaatccga tcggcgggcc gccgaagatc gtgtgcatcg gtccggtcac cgcggcggcg
     9361 gcccgcgacg ccggtctgcg gccgaaggcc gtcgcccggc ctcacaccct cggcggattg
     9421 gtcgacgcgg tcgagcgagc gctcggctag acccagacct cgcggagggc acggagaacg
     9481 ttgccgccga cgatcttcgc gatctgctca tcggtgtagt cgtggccgac gagccagcgg
     9541 atcacgttcg ggaactccgc tgggttctcc agcccctcga cgtactcgac gggctcgtac
     9601 tcgggcacct cgcccgtatc gaacgccgcc gcgtacatgc cgtggagccc cacgtggtcg
     9661 ccgaagaacg tgtcgggacc gaacccgacg tgatcgatcc ccatcacgtc gacgcagtat
     9721 tcgaagtggt ccatcactcc ttcgagcgaa tgccgcgggt gggccggcga gatcgtcgtg
     9781 tgcggcgatc cctcgatccc gataacgccg ccggtctccg cgaccgcctt caacaggtcg
     9841 tcgtgcttca gcttccgctc ggagaacagt gcccgcgcgc ccgagtgcga caggaagacc
     9901 ggtcgctcgc tcacctcgca ggtctgcatc gcggtgacgt cgcccgtgtg cgagacgtcg
     9961 atcgccatgc cgagccggtt catgcggcgg accgcatcga ggccgaaccg ggtgagacca
    10021 ccgtcgccgg gatccttcac tccgccaccg agctggttcg actcgctgta ggcgaccccg
    10081 cacatccgga cgccgagccc gaagagcacg tcgagacggt cgagctcgtt ctcaatcatc
    10141 gcggcgccct ccatacacgg caccatcgcg atccggccgc tccgatgggc ctcctcgatg
    10201 tcgtccacgg tgccgccgac gaagacggtc gactggtgcg cgacgtcggc gaggtgcatc
    10261 ccgaggtcgt ggatcacatc ggtccacttc cacccgctct tcgacgtgat cgtcgcggtc
    10321 ccgtcgagga agttctcgaa gacggcgtct aggccggagg cggccagtcc ctcgtagccg
    10381 taccactcgc ggccctcgcg gacgtactcg tcgttctcgt tcatgtccac cggcgtgatg
    10441 ccgcagtggt cgtggagcga gatgcacacg cacccatgga gcaggcggtc ggctcgttcg
    10501 ttctgctcgt cggtgagcgg gtagacgaac ggctccacgc gaccgatctg cggcgagagc
    10561 gcgaactcct tgtagtcgac gcccgcctcc aagtacgaga acgacacgta gccgtcccat
    10621 cgcttcttcg gcttcatcgg catcctttcg ctatctcctt cgaggtacgc tctggcccgt
    10681 gagcttcccg cattcccgcc cccgtcgcat gcgccgcacg ccggcgcttc gatcgttgat
    10741 acgcgagaac gacgtgcggc ccggcgacct catcgcgccg ttgttcgtga aggaggagct
    10801 gactgagccc gcgccgatcg cgtcgatgcc ggggcagagc cagcacacgc tcgagtcgct
    10861 ccggaaagag gccgtcgagg tcggggagcg gggtgtccgg gcgttcatcc tgttcgcggt
    10921 gccggcccgg aaggatcccg agggatccga ggcgtggaac ccggaaggga tcgggcagca
    10981 ggcgatcgcg gcgctccggg cggagttcgg cgatgacgcg gtcgtgatcg gcgatctctg
    11041 cctcgacgag tacaccgatc acggtcactg cggcgtcctg aatgagcgcg gcgcggtggt
    11101 gaacgacgcc accgtcgagc tgtaccagcg gatcgcgatc gcacaggccg aggccggcgc
    11161 ccacctcgtc gggccgagcg ggatgatgga cggacaggtc gccgcgatca gatcggccct
    11221 cgacgccagc gagaaccagg acatcgggat cctcgcgtac gcggcgaagt tcgcttcggc
    11281 gctctacggc ccgttccggg aggcggccga gggagcgcca cgcttcggcg accggaccgg
    11341 ctaccaacag gaccccgcga acctcgacga ggcgctccga gagatccgca cggacatcga
    11401 cgagggcgcc gacatcgtga tggtgaagcc ggccctgccg tacctcgacg tgatccgccg
    11461 cgcgaaggac gagacgcggt tcccgatggc cgcctaccac gtgagcggcg aatacgcgat
    11521 ggtgaaggca gcggctgcga acggctggct cgacgaacgc cgcgtcgtcc tcgagctgtt
    11581 gacgtccatc cgacgggccg gcgcggatct gatcctgact taccacgcga aggacgcggc
    11641 ggactggctc cgcggctgag ccgccgatgg ctcgctacgt tgcgctgctg aggggtatca
    11701 acgtcggggg gaagaacgcg gtcccgatgc ccgcgctgaa ggcgagtttc gaggacgccg
    11761 gcttcggcaa cgtcagtacg tacatccaga gcggcaacgt cgtgttcgac acgccgtcct
    11821 cgagccagga agagctcacg cgcaaggtcg aagggatcat ccggaaggcg ttcgcccact
    11881 acgacgcgag cgtggtgctg cgcagtcgga gccagatgcg cgctgtcgtt cagaaggcac
    11941 cgaaggggtt cggcaccgag ccggcgaagt accgctacga cgtggtcttc ctgaagccgc
    12001 ccctgaccgc gaagtcggcg atcaagaaca tagcgacgaa ggaaggcgtg gaccgcatct
    12061 gggcgggcac cggcgtcctc tacatgtcga gactcgacag ccaggcgacg cagagtcgtc
    12121 tcaaccgcgt cgcttcgctc cccatctaca agcacatgac gatccggaat tggaacacga
    12181 cgacgaagct cgcggagctc gtgtcctgac gcgcgtcacg cggtgaccgt cgacgggtcg
    12241 cagttcgccc cggacagcac gacgcagacg cgttccccag gcgagaccgg ataggcgcct
    12301 gagcggagcg ccgcgaacgc ggcggctccg cccggctcca cgatcagccg cacctcttcc
    12361 cagagggcgc gctgcgcctc ccggatctgt tcttccgtga ccagcaccat ccgctcgacg
    12421 cgatcgcgga cgatggggaa cgtgtgatcg cccacccgag ccgccccgag tgaatcggac
    12481 gcgagaccgc ccacagggac agtgacgggt tcgccggcag cgagcgccgc ggtcatcgaa
    12541 gcgcatccct cgggctcgac gccaaccacg cgaacgcgat ccccgcacca tgccgcgatc
    12601 cccgcgagcg gtccgccgcc gcccacgccg atcatcacgg tgtcgacgtg cagaacctgc
    12661 tcctcgatct cgaccccgag cgtcccctgc ccggcgatca cctcgggttg atcgaacggg
    12721 tgcatcagta aagctcctgt ctcgacggcc ctcgcgccca gcgcctcgtt cgcctcggcg
    12781 aagtagccct cgatgatcgt cacggcggca ccctgggcgc ggacgcgatc gatcttcgtc
    12841 cgaggggagc tcgacgggat gaagatctcg gccggcacac cggcctcgcg agcggcgtag
    12901 gcgaccgcaa ggccgaagtt cccgccggag gcggcgatca cgccggccgg cggtacctca
    12961 ctcgcgagca gcttgttgaa cgcccctcgc ggcttgaacg accctgtgta ctgcaggagc
    13021 tcgagcttgg ccacgaccga cgcgccgcgc aactcaccga gctcgagcac gggcgttcga
    13081 cggatccgac cggcgatccg ctcggcggcg gcgcggacgt catcgctccc gacggtcatg
    13141 gggtcgcatc caaccacagc catagactct ccgggccgat gaacggatcc gacgagctgt
    13201 tcgcacgggc gatgctggcg atccccgggg gcgtgaactc accggtgcgc gccttcggcg
    13261 ccgtgggcgg cgtgccgcgg ttcgccctcc gaggcgaggg cgcctacctg gaggacgcag
    13321 acggcgcgcg gtacgtcgat ctctgccagt cgtggggcgc ctcgatcttc ggccacgcgc
    13381 ggaaggagat cgtggacgcc gccgcggagg cggcctcccg agggacctcg ttcggcatcc
    13441 ccaccgaggc ggaggtcctc ctcgccgagc ggatcgtctc catggttcct tcgatcgagc
    13501 aggtgcgact ggtctcgtcc ggcaccgaag cggcgatgtc tgcgatccgg ctggcacgtg
    13561 gcgccaccgg caaagatgcg gtgatcaagc tcgagggctg ttaccacggt cattccgacg
    13621 ccctgctcgc gcacggcggg gggagcgggc tcgcgacgtt cgggatcccg agcacgcccg
    13681 gcgtcaccgc cggtgccacg cgcgacacgc tggtcgcgcc cttcaacgac ctcgaagcgg
    13741 tccgggcgct cctcgatgcg aacgagggga gcgtcgccgc gctcatcgtc gagcccgttc
    13801 ccgcgaacat gggtgtggtg ccacccgacg acgggtacct cgacgggctc cgtcagctct
    13861 gcgacgagca cggtgcgctc ctgatcttcg acgaggtgat caccggattc cggatcgccc
    13921 ggggcggcgc ccaggagcgc ttcggggttt cccccgacct caccgtcctc ggcaaggtga
    13981 tgggcggcgg attcccgtgc gccgcgttcg gaggccggcg cgacctgacg gagcagctcg
    14041 cgccggcggg ccacgtgtac caggcgggga cgctgagcgg gaaccccgtc gcggtggcgg
    14101 ccggcctggc ggccctggat ctcatcgacg ccgaggaccc ctatcccggg ctcgagaccg
    14161 ccgcggagga cctccaggcc ggtctcgctg gcgccttcga ggcgaacggt atcggcgtca
    14221 cgatcaaccg agcaggatcc ttgttcagcg tcttcttctc cgaggggccc atcaggaact
    14281 tcgatgacgc gaaggccgcg gaccacgagc ggttcgcgcg gttcttccac cacatgctcg
    14341 ctgagggcgt gtacctcccg ccgtcgggtt acgagttgtg gacgctctgt tcggcgttcg
    14401 gcgaggcgga ggaagaagcg gtgctcgggg cagccggtcg cttccgcgac tgaccgcccc
    14461 gacgctcaca ccagcgtgag ggtggtgtcc tgcaccgtct ccagctcgtc gccgtcggac
    14521 gccggctcgg tggcgatgca gaacgtcccc acgatgtgct cgcccgcgtc gtgcgtcggc
    14581 gcgacacggc accacatgcg cccctccatc cccgggaggc tgaacgtgac cgtctcaccc
    14641 gtgagggcgg ccgcgtgggc ctcgaggatc gggaggctct ccccttccat cccgaacgct
    14701 tccaggagct cacggccgtc gaggaagcga gggtggatcc ccagatgttg ggatgccacc
    14761 cacgagaccc cacggaggcg gagcgagcca tccgtcgtcc acaggaccgc cgggtagtga
    14821 tccccgtcgt cgcccgatgg gaccggatca cggcgaggtg gggaccagtc caccgtgtcc
    14881 gtgatcccga tgagctccca ctgtcgcatg tgcctctcct cctcggtgcg gctccccctt
    14941 ctctacccga tatcgggtag ctgcctcccc gggagtatcg gcgaagagga ccatttcgcc
    15001 cttccccgag gggactaggg cttccggagg aggagtggcc ctgcaacgga tgcaccagcg
    15061 ctggcactat ggaggcgaga gccatcgaca agagaggacc gacatgccaa ccgagatcgc
    15121 caccttcggc gccggctgct tctggggcgt ggagcacctg ttccgaggga tccccggagt
    15181 caccgaagcc atctccggct acagcggggg cttcgtcgag aacccctcgt accagctcgt
    15241 ctgcacggac acgacgggcc acgcggaggt cgtccaggtg acgttcgatc ccgagctcgt
    15301 ctcatacgat cagctcctcg aggtcttctg ggcgatgcac gatccgaccc agttgaaccg
    15361 gcagggaccg gaccgagggt cgcagtaccg gagcatcgtt ctcgcccatt cggacgagca
    15421 gcgggcggcg gcggaagtgt cccgggaccg ggtccagccc cggtacgaac gcccgatcgc
    15481 gacggagatc cacccgttcg tggccttcta tccggcggag gactaccacc agcgctacta
    15541 cctgaacaac ggacacgagc cctactgcca ctcgatcccc gcggggctgc tcgaggagct
    15601 ggggctcgtc cggacctagc gtcggcgagg cgaaaccctg ccaagctcca cggcgtgaac
    15661 gaggcgtcgg cgcggccgaa cgatctccag ccgttcctgg accggttcgt cacggcatgc
    15721 gagcaggacg accgggtcgt ggccgcgttc ctcggcggct ctcgtgggcg tggagaggcg
    15781 gacgtgtact ccgacgtcga cctctgcgtg atcgcgaagg acgacgcgta cgagacgctc
    15841 atcggcgatc gtgccgggtt cgtcgggcgc ctcggggcgc ccctcttcct cgaggacttc
    15901 gggttcgcgg gcctcgtgtt cttcgtcctc gccgacggga ccgagggcga gctcttcttc
    15961 gggagcgagg ggcgactgga ggagctcgag ccaggtccga cgatccgacc gctgttcgac
    16021 ccgggcggga tcctggaggg cgtcgcgttc gccggggagc gggcggatcc cgcggagcag
    16081 cacgtagtgc tccgacagat cctcgcgtgg ttctggcacg agctgtcgca cttcatcgcc
    16141 gcgatcgggc ggggcgagct ctggtgggcc gccgggcagc tcgagtcgct tcgcggtcac
    16201 tgcgtgaacc tcgtccggat cgagcagggg gcgttcgccg gggaggagcc gtacgagaag
    16261 atcgaccgga cgatcgatct cgccgcgctg tcaccgatcg tcgagacgtt cgtgccgatg
    16321 gagcgggacg cgctgctgcg agccgcacgg gagctcgtcc gcttctatac ggagaaggcc
    16381 ccgggcgtcg cggaggcgaa cgaggcgacc tatccgctcg agcttgagga gctgatgatc
    16441 aagcgcttga cgaccttgcc ggctcgcgct agccgtcgcg gaagccgacg agcctgtgcg
    16501 agccgtcgca gaacggcttg ttctgtgagt acccgcaccg gcagagggtc tggcggttgc
    16561 gaacctcata ggcctcgccg tccgctccaa cgacccgcac cccgccgcgc acgaacaacg
    16621 gtccgttccg gaacacaccg atctcgggtt cgtactcggg ctcatggtcc ggcgagtcca
    16681 cagagtccgc ccacgccagg cgtccggagg gacagagctg gaccatgcgg cccatccgtt
    16741 cgcggacggc gggatccggg tcaccgagca tctgccaaac ccccgtgaat cggtccttgc
    16801 agtagccggc cttcgtgcag atcgagagat cgtcgctgat gatcgccccg tcccgagcct
    16861 ggaagacgtc ggtccggtcg tcgcgcggac cgtgatccgc gcgctccgtg acgtcgacac
    16921 ggggttcctc gaggtggctg tcgtcgcaga acggcttgtt cccgctcttg ccacagcgac
    16981 acaggcggta ggtctcgtcc gcttccaagg gctcgaaggg cgcgtagtcg accggttcgc
    17041 cgtactccgt cttgacggtc ctcgtccggg cgatcggcag cgggcctttc acctcgtacg
    17101 ggccgttctc ctcgatccgg atctccgggt ccgacatggg cggcatcctc ccacgtcgta
    17161 ccatccgcgt gtacccagca ggctcggagg aggggacccg tgtcggccgg cacgatcgac
    17221 gagtacgctg aggccctcgg cgactggcgc ggcgaggtcg tcggcgagct cgatgccctg
    17281 atccggcgcg cggctccgaa ggcgaccggc tcgatcaagt gggctcagca cgtctacgag
    17341 caggacgggc ccttcgccta cgtcaaggcg ttcacctcgg ccgtcaactt cgggttctgg
    17401 cgaggtgccg acctcacgga caccgaaggg cgtctcgagg gaaccggcga tcggatgcgc
    17461 cacgtgaaga tccggacccc cgaggacttc gacaaggccc gatccaccgc ctgggtcaag
    17521 gaggctgtcg agttcaacgc cgagaagggc gaccccacca aggcgcgcta gctcgaaggt
    17581 cctcgattcg gtccgccacg acgactatcc ttcgaacgcg tgttcgagga actcaacccg
    17641 gagcagggcg ccgccgtgcg acatggcgaa ggaccgctcc tcgtcgttgc cggggccggc
    17701 agtggcaaga cgatgacgct ggcggcgcgg gtggcctcgc tgatcgagcg agggacgagg
    17761 ccggagcgca tcctcctcct cacgttcagc cgccgcggcg cgagggagat gttgtcgcgc
    17821 gccgagcggc tcaccgcgca cgacgacgcc ggccgtgtgt ggggcggcac gttccacgcg
    17881 gtgggcaatc ggctcctccg tctgcacggc cgcgcgctcg gtctcgcgcc cgacttcacc
    17941 gtcctcgacc aggccgacgg cgccgacgtg atgaacctcc tccgcgacga gctcggctac
    18001 tcgtcccgcg agcggcgctt cccgcgcaag gacaccctcg cggcgatcta ctcgcggacc
    18061 gtgaacgccg gcgagcggct cgtcgacgtc ctgaagggtc actatccgtg gtgcctggac
    18121 gaagccgaag gcatcgtcga catcttccgc gcctacaccg aacgcaaacg ccgacagcat
    18181 gtcctcgact acgacgacct cctgctgttc tggaaggccc tcgcgacctc gccggccacg
    18241 ggggcgcagc tcgccgcgat gttcgaccac gtcctcgtcg acgagtacca ggacacgaac
    18301 gcgctccagg ccgacatcct cgagggcatg cgcccctctg agggtccgcg gaatctcacg
    18361 gtcgtcggag acgatgccca agcgatctac gggttccgcg ccgccaccgt gaagaacatc
    18421 ctagagttcc ccgagcgttt ccccgggacg acggtgatca agctcgagcg caactaccgg
    18481 tcgacccccc cgatcctcgc ggcgtccaac gcggtgatcg ccctcagccc gcaacgacac
    18541 gagaagacgc tccggtcctc gcgcgaaagc gagttgcgcc ccgtgctccg tgcgtgtctc
    18601 gacgaagccg agcaaagcga cgccgtgtgc cgagccgtgc tcgggtatcg ggaggaaggc
    18661 gtccccttga aggcgcaggc ggtcctgttc cgcgccgctc atcactcgga ccacctcgag
    18721 gtcgagctcg cccggcggaa catcccgttc gtgaagtacg gcggcctgaa gttcatggag
    18781 gcggcacacg tcaaggacac gctcgcgtgc cttcgggtcc tcgagaaccc attcgacgag
    18841 gtgagctggt tccgggtcct ccagctcctc gaagggatgg gccccgcgtc ggctcgccgg
    18901 ctgatggacg atctcgaggt gcgtcggacg gacgatgccg atgccacgtc gcccctcgca
    18961 cggttcctgg cgcagccgct cgcggtcccc aagggtgcgg cgcaggggat ccgagacctt
    19021 cgcagcgtcc tcggcgactg tctggaggca gagaccgtcc cgccggccgc ccagatcgcg
    19081 cggctccgga cgttcctcaa gccggtgatc gcgcggaagt acgacgcggc cgccgcacgc
    19141 acccgcgatc tcgaacagct cgagctcctg gcaaccgaat ccgcttctcg cgggcagttc
    19201 ctcgccgacc tgacgctcga tccgccctcg tccaccggcg acctcgccgg cccaccgcat
    19261 ctcgacgagg atcacctgat cctctcgacg atccactcgg cgaagggcct cgagtgggac
    19321 gtggtccacg tgatccacgc ggccgacggc atgatcccgt ccgacatgtc gacgggggac
    19381 gacgacgaga tcgaggaaga gcgacgcctc ctctacgtgg cgctcactcg ggcgcgcgac
    19441 gcgctccaca tcacctaccc ccagcggtac taccgtagac cgcggggcct ggaggatccg
    19501 cactcctatt cccagcggtc gcggttcctg ccggacgagg tcctcctcca cttcgaggtc
    19561 atcggcccgc acgtgctgtc ggtcgccgac ctttctgagc cggtgacggc ggcggccgac
    19621 gtggatgcgt tcctcgccgg cctctggacc gactgaaccc ggtccggcgc ctgtccgtag
    19681 accgatcgtc gcggaggctc ccgacccgcc tccgccgggg agggaagctc catgcatcga
    19741 tcccgccgca tcgccgcggt gctcgtctct atcggcttgg cgaccctggc cttcaccctg
    19801 cctgcatccg cgggaggtcg tccgttcacg acgtcgctca gtggggcggc agagcggccg
    19861 accgccgggg atcccgacgc cacgggaacc gcgcgggtga ccgtcaaccc aggtacccgg
    19921 gaggtgtgct ggtcggtatc cgtcgacaac gtcgacctgc cggtcatcgc ggcacacatc
    19981 cacatcgggg gcgcggacac gttcggtcca ccggtgatct tcctgcttcc ggaaggcgtg
    20041 tccgacgccg atggtgagtt ctcagggtgt tcgacgatcg atcggagcct cgcgttcgcg
    20101 ctgctcctct ctccggagga ctactacgtg aacgtgcaca cggatctgtt ccaagcggga
    20161 gccgctcgcg ggcagctctg agtctgccta gcgacgggtc ggcggcccga tccgggccgc
    20221 cgaccaccaa cccctatcct ggagggcgtg gaaggcgcgc tcctggatct gtacgacacc
    20281 gtcgcgcgga cgcgctggcg cgagctgtcg gaccggatct ccggtgagct gggggtggac
    20341 gtcgcggcgc tctaccgtgc ctacggcctc acgagggcgg agcggagcat cggcgccttc
    20401 gacgggccgg agggcgacat ggcggcgatc gtcgaggcgg caggatccga atcgacgccc
    20461 gagctgatcg actccctggt cgcgatggag cggcagttca cggaaaccgg cgtcgagctc
    20521 tgggaggact cgctgcctgt cgtctacgag ctccggcaac gaggcatcaa gacggcattg
    20581 gtcagcaact gctcgcactc gacccgcggg atcgtcgatc gcctcggcct cgtcgacgcg
    20641 ttcgacgagg tcgtgctgtc cttcgaggtt gggctccgta aacccgatcc cgcgatctat
    20701 cgggaggctc tcgggcgcct tgatctcgaa ccgggtggag ccgtattcgt cgacgatcaa
    20761 caggagtact gcgacggcgc tgccgcgatc gggatcgaga cctatctgat cgaccggtcc
    20821 ggtgatgcca cgcccgacga gaacgggcac cgggtgatcc gggacctgcg ggcgctccta
    20881 gccgtaagcg cctgacttct cgccggccgc cccctcgttc atcacttccg tgatgtcggt
    20941 gacctcggtc gctttcgggc gcttcacgtt caccggctcg gcgaacgccg agtagtcgac
    21001 cgtcatctcc atctgcgcgt cgcccgcccc gggcacgagg tctccgaagt ccatcgtgat
    21061 ccgctggcgg cgaggcaggc catcgtcgtc gatccagatc tggaacggga tgtcaccgtc
    21121 gatcccgagc gcctcgaact gctcgaccgc ggcttcgagc tgctccgtcg tctccgcatc
    21181 ggcatcatca cccacgatct cggcgaagcc ctcgagcacc ttctggaggt cgatcgtccc
    21241 ggtgtagcgg gtggtggcga tgccgtcgat gtccgtctcg cctgaggcct tcacgtcgaa
    21301 gatcccagcg aacaacccgg cgtaggcgga cgggtcggtc gtccccgctc cgaagccgcc
    21361 gaaccgggcg gccgtggcag gatccatctt cgacgggtcg aagctgaccc aaggggtcgg
    21421 cgcaccctcg aagacgggcg agcgcatgta gatcaccccg tcgtccatga ccatctccac
    21481 ctcgccgccc atcatcccga ggtccatcgt catcgctact tgccgggtcg cgctgtcgac
    21541 atccgcgtcc gccgtgatcg agaaggtctc cccctcggcc gacatcgaca tacgcagctc
    21601 ctggtgatag gtccccgcct cgtacaccgc ggtcatcgca tcgagcacgg cgtctcgcgg
    21661 actgacgggg gtgcctcctt ctgcgcagga ggccagcatg cacagggtcg tgagggagag
    21721 caggagcttc ttcatgcaac catcatcggc acgaggagcc cctcagtcca cccacggacc
    21781 gaagatcgtc aacggagatc gtcgaggacg tcggcgatgg acttgcggat ccctgtgaag
    21841 aaaggggtct cgaccttcgt gtagaggcgg gtcttggagt cgcgaagcct gcggatgcag
    21901 tcgacgagcg cgtcgagctc atcggtctcg aacgcgagga tccattccca gtcgccgagg
    21961 ccgaacgcgc tcgtggtgtt cgcgaggacc tgcgggaact cgcgaccgag gaccccgtgc
    22021 tccgcgagga gctcgcctcg ttcctgcggt gggagcaggt accactcggc cgagcgtacg
    22081 aacgggtaga cgcagaggta cgtgcgcggg gcctccccgc ggacgaacgc cggctggtgg
    22141 tccggcgtga actcggcggg cttgacgaga cccatcaggg tgacgaccgg gtcgaggaga
    22201 tcgcccgccc gggtcctccg gaactcgacc aggaacctct ggagatcctc ggtccccgcg
    22261 gcggtcagcc acaggacgag atcggcgtcg gcccggaacc ccacggtcga atacgagccg
    22321 cgcacgtcca cccggcccgc ccagtccttg aagagggact cgatctcgtc accggcgttc
    22381 cgcagcgcct ggtcctcgaa cagctcggcg cgcaactcct ggctcgcggc gaacacgggg
    22441 tacagcgcgt agatcgtctc ggtcatgccg gctccttcct ccttcctggc cggggcgcac
    22501 ccgcgtgcgc gagcacctgg tccgccgtct cttcggcggc gcgagccaga tcggacacac
    22561 ccacgccgtc gaacgcgctc ccgacgagga agataccggg cgggaggccc tcccggatct
    22621 cctcgacgag ggcgccgtgc ccacgctcgt actgaggcat cgctgcccac catcgatgca
    22681 cgcgggctgc cacgggggcg gggagatcga gcgcggcgct cagatggcgc acgcacgctc
    22741 cgacgatgtc gtcgtcgggc gcgtcgagga cgtcctcgtc acccgccccg cccacgtagc
    22801 agcggaccac cgctcgatcg ccgaacgacg ggttcggcca cttccgcgac atgaacgtgg
    22861 ccgcggtcat cggggcggcc ccgcgcggga cgacgaagcc ggtcccgtcg ggcagggcgc
    22921 ccgcggttcc ggccgggtac acgaggaaca cgacgccggt cgagacggag ccgatggtcg
    22981 agagcccggc gggcgcgctc ggcccgagca aggatgccga gaccggtgcg ccacacgcga
    23041 gcacgacggc gtcgacgtgc tccgaggagc cgtccgcgag gtcgaccgtt cgagccgtga
    23101 caccggtcac ctcgacaccc gtccggaggc gctcccccac cgtcgcggcg agcacgtcca
    23161 ccagttcccg catccccgat cgaggtcgaa ggaacagggg tcccggatcg gcgcgacccg
    23221 cggcccgctg cgcggcctga gcaccgcgga tcaggcttcc ctgggactcc tcccaagcca
    23281 cgagttcggg gaaggtggcc ggcgcggaca accgatcgac atcccccgcg tggagcccgg
    23341 cgagcagggg cgcgatcgct cgctcggtgg cctcgtcgcc gaaccggcgg cggagcagct
    23401 cgccgagcgt ctcctccgcg ccgtgccgac gcgtccgctt gacgagatcg gcgagggcgc
    23461 gaacgcggcc gcgtgacgaa acccccggcc accgcaggac gtccccgacg tcgcccggga
    23521 tcccgaatgg cgctccgcgc aggtacggca ccagaccgga ctcggtccac aggaacgctc
    23581 ccgtcgtgcc cggcgggaca agcgtcagcc cgaggtcccg acagagcgcc gacccccagg
    23641 gcttcctcgc cgccagcgaa tcggggccgg gctccaacgc gaggtcgccc accggaacgg
    23701 ggggcacgat gatcccaccg tacgtgctgg accgctccag cacgacgggc tccacaccgg
    23761 ctcgctgcag tcggaacgcc gtggcgagcc cggcgatacc gccgccgacg acggcgaccc
    23821 gcggattcac ggagccgccg tcaccaggtg gtctcgcacg acgtccgcca ggacctccag
    23881 gaacgcgggg tcgtcgttcg gcatctcggt gcgggcgaac cggatgcccg ctccctcggc
    23941 gagcgccttc gcctcgacgt ccaggtcgta aagggtctcg agatggtcgg ccacgaagcc
    24001 agcggagcac acgaccaccg cgggatggcc catcgccgcc gcttggagga tcgactcctc
    24061 caccggcggg ccccaccacg ggtcggaggt ccggccggcg ctctgccacg cgatgccgta
    24121 gctctcgaga ccgagcgtcc cggcgacgag gtccgccgtc tcctgcagtc cggcccggta
    24181 gcggcacgat ccgtcgcaca tgcacgtctt gcagcggagc gacccatcct cgacgacgcg
    24241 ggtgggcagc gagtgcgccg tgaacaggac ccgcgtggcc gatcgctccg ccggcggcac
    24301 gtcctcgagg gcgtccgcga cacgggcggt caggaaacgc acgaacgagg ggtgcgcgcc
    24361 gtactcgcga acgaacgaga acaccggccc tcctccctgg tcggccacgg cagcgagtac
    24421 ccgctcgacg taggtctcca cggacatccc cgaccagtgt ggagccatca cgatgccgac
    24481 ggcccgctcg acgccctccc cccgcatctg cgccacggcg tccgggatga acggcggcga
    24541 gtgcttcatc ccgaggaacg ctcggaacgg ggtcccgtcc tcgctcagtc gggcgaccag
    24601 gccctcggcc tgcacccggg tggtgtccaa cagagggaac acgttgccga tcgcttcgta
    24661 gcgagcgcgc aggtcggcca gatgttccgg cgaaggaacc cgtcccccgc ggatgtccgt
    24721 gtagtagcgc tcgatgtcct cgggtccgcc cgcggtcccg tacgccatga ccagcacccc
    24781 tgtcggggcc gtcacggagc cggtctctcg gtctcggcgt gaacgagatc gacgagccgc
    24841 tgcaagtgct cgacgggcgt atccggcagc actccgtgcc cgaggttgaa cacgtgcccg
    24901 tcgcggagcg ccgcgcgtgc caacacgtcc tcggccttcc gctccaccgc gtcccagtac
    24961 ccgagcagga ccgccggatc gaggttcccc tggatcgccc ggtcgtgtcc gatcacggcc
    25021 caggcgtcgt ccaacggcag gcgatggtcg acgccgatcg cgtcaccacc cgcgtcccgc
    25081 atgaggtcga ggagatggct cgtgccgacc ccgaagtgga tcgccgggac gcccatgccg
    25141 gcgatcgcct cgaacaccct ggccgtatgc ggcatcacca tgccgcgata atcgtcccga
    25201 tcaagcgcgc cggcccacga gtcgaagagc tgcaccgcgt gcacaccggc gtccacctgt
    25261 gcgcggagat gcgccacgac gatgtcggcg agcatcctga ggagccgatc gaacacggcc
    25321 ggctccgtga agagcaaagc cttcgtccgg gagaaggtgc ggctcggtcc gccctcgatc
    25381 agatagctcg cgagcgtgaa cggagctccc gcgaatccga tcaggggcac ctcgagctcc
    25441 ttcacgagga gcctcacggc ctcggccacg tacggctcgt ccgtctcggg atccaacggc
    25501 cggagccgct cgatcccgga cgcgtcccgg agcggggggt cgatcaacgg ccctcgcccg
    25561 gcctcgatcc ggatcggcac gccggtcgcg tggaggggca ccatgatgtc ggagaagagg
    25621 atcgccgcat cgagcggcat cctgcggagc ggctggagag tgagctccac gacctcgtcc
    25681 ggacgacggc aggtctcgag gatgtccgcg tccccgcgac gtgctcggta ctccgggagc
    25741 gaacgacccg cctggcgcat gaaccagacc ggcgtgcggt cgaccgggcg gcgtcggagc
    25801 gcgaggagca gccgatcgtt gggaggtagt ccggtcacgc ccctcaggat acggagctgg
    25861 cttggaccgg cggtgtgggc gcggcgggtg ggccggccgt gtccatgagc gcgtcctcca
    25921 gcatcgagcg cttcggccgt gggaacagga tccggaacgc ggcgcccccg ccgggagcct
    25981 cctcgatccg aacgtcgcca cccatcgctt cgacgagccc ctggaccacc gacagcccga
    26041 gaccgaggcc gtgcccgctc atcgagtcga gccgctcgaa acggttgaag accctcgccc
    26101 gatcctccgc tgccacgcct cgaccggagt cgaggacggc gaacccgaca tggccagagt
    26161 cctcggtgac cttgatccgc accggcggcc ggccgtactt gaacgcgttg tctatcaggt
    26221 tgtcgaggat cctccggaga tactcacgat cacagacggc gatggccggc tgggaggcgt
    26281 cgacctggag atcgcgatcg aggaccctgt ggtccgcggc cgcctctcgg acgaccttgg
    26341 cgaggtccac cggttcgagc cggttgacgg cctcccctcg ctccagctcc gcggcggcga
    26401 gcagatcctc gaccatggtg ccgagtcgaa gcgcggctcg ttcgacgatg tcctcgagct
    26461 caccgcgatg ctcgtggagc tcaggcttcc gcgcggacga caccgcgccg atgatcgacg
    26521 cgatcggcgt gcgcagttcg tggctcacca gggccacgaa ctcgctcttc aagcggttga
    26581 ggtgcgtcag ctcctcgacc tgcgctcgct cggtctcgta gagacgggcg ttcgcgatcg
    26641 ccgcggctgc ctgctccgcg aacagggaga ccgcgcgaag gtcgaactcc gagaagaggc
    26701 ggtctcccgc ggagttcacg ttgaggacgc cgagcacctc ctcacggtgg atcagcggtg
    26761 cgctgatcgc actcgacacc gggagcgaat ggggcaccag ccccgggaaa tcgcccggat
    26821 cggccttgcc ctgcaccagg agggcttctc tcgtcgatgc cacatgtccg gcgatgcctt
    26881 cgccgagctg cacgctacgg ccgtccgcat gctcgttgcc gtgggaacac gccaccacca
    26941 ggtgcgcgcc ctccaggagc atcaccgagc cgctgtcgct gtccaagagc tccaccgcgc
    27001 tgcggagcat cgtgtccagg acgacggaga gctcgagcac gtcgttcatc gccttgcccg
    27061 caccgagtag cagctcgacc tcgcgcaggc ggttcgacag agccgtggtc aacacccgct
    27121 cgtcgatcag gagacgtgag agcgtgcgga ggtgtcgctc tttgtcgatc gcgtagatgc
    27181 agaacgcgat gctcagcagc acgacggcga tccgcatgac cggggtggct gcgaacgccc
    27241 gtgtggcgct cgtctgccag gtcgacacga agacgacccc gagcgagatc gagaccagca
    27301 gtgtcgccgt gaggatccag agctgcagcc tgcgcttctc gacggcctcg agcgacggcg
    27361 ttcggaagtc gcgcaggccg gccacacgag cctcctgctt gatgtgctcc gtcaccgttt
    27421 caccggcagt catgcatcaa gctcctgctg caagcatcgg acgtcccggc aggcgggttg
    27481 agcaggggga agttcggatc cccgtgccgg acgatcaggg cttccgcgcc gacgggctgc
    27541 gttgtggcgt gacgacctga cgtgagcgcg tgagactgga agatgcgagg aaggagcgca
    27601 tatcgcgagc accggcatcc tgatcgcgac gtacgagccg gcgcgggctc aggtcggatg
    27661 gtgaccgtcg accaggtccg agcgttcgcg ctcacgttgc cgcgcaccac cgaggcgctc
    27721 gtccgggatc gggtcaagtt ccgggtgggc cgcatcgtct acctcgcgtt ctctcgcgac
    27781 gagacgacga tgggcttcgc gttcccgaag gaggaacgtg aggcgctcgt gcggtcggag
    27841 cccgagcggt tcctgatgcc ggatcggtcc gacctgcgct tcgactgggt cgtcgttcgg
    27901 ctcgattcga tcgatgccgc cgagatgcgt gagatcgtcc tcgacgcatg gcggatggtg
    27961 gtgcccaagc gggtggcggc cgcgtacgag gactcggtcg gttgagtctc atgccggggg
    28021 aagcgggcac ggaagcgcac aggatccatg cgtgcgcacg tacagccagt ctgggatgtc
    28081 tccgggaacc gtagtcccag tcggaccagc ctgctcgaca tcgtttccct gatgcgatac
    28141 cgtaggtgag cgcccgcgat ctggcgaacc acagggcgac gttccgaaac cgtctcgatc
    28201 cttcggtgta tccatgcttt ccgcacgtcg gcacgccgat tcgagggcgg tttggccgag
    28261 gggcccgcca cgaggtgcac agaggagggg atcaggtgac agggatcgag atgcactctc
    28321 tcgcaacgcc aaggggaagc aggatgacgg atgacccgga tcggcggata gaaggcggtc
    28381 cacagccgct cggtatcgat cggaacaggg gaaaggccga cgaccaaggt cggctccgag
    28441 aacgagacga ggaggttgat gcatgaagag gctcatagtt gtttctcttg cgctcaccac
    28501 cgcactcatg atgttcgggg ggatggcgcc ggcccaagcg gccgtgacgc aagcggtcga
    28561 cgacgacggg atggcatcgc tgaccagctg caacgccgtg acaccgacac acatcaccat
    28621 ccaggcaggg gtgaacgccg cagccgccgg agacaccgtc aaagtctgcc cgggcaccta
    28681 caccgagaac gtgacggtga acaaggctct caccctgagc ggcgcgaagg cgggcgtgaa
    28741 cggcacgcgc gtcgtcaacc cggccaatga gtcgatcgtg catccgtcgg accccaacca
    28801 gccgaccttc atgttggagg tcagcgcgat cacggtgaat gggttcctca tcgagggcaa
    28861 cacgaacaac gcggggatcc agacccgtcc caccgcgtcc ggctaccaga tcctgaacaa
    28921 catcgtgcgc gacaacgtgt tcgggatcta tctgcacggg gatggcgcca ccaccacact
    28981 cgttcggcgg aacctgttgg acaacaacaa ccggccgggg gcggcgtcgg gcaatgggat
    29041 ctactcggac caaggagcgc tgggcatcac gatccaggcg aacagcttcc ggaaccacac
    29101 caacaccggg atcgtcttcg cgaacgccgc ggtcgtgcag gagagcatca tcgttcagaa
    29161 caaccggagc ctgaacgaca acacgttcgt tgcgctcttc gacgtgcaga acgcgacggt
    29221 cgtcgcgaat cagaccaacg atacggtcga tgcggacgac ttcggttcga cgatcttcgt
    29281 cggcggcgac tccgccggga tcgtcatcca gcggaacgtg ctgacgaacc ctgggttctc
    29341 agggatcgcc gttcgtgatg tactcggcac tgccggctcc accgcgaacg tgaacgtgct
    29401 ggggaatacc gtcaccggag cagaaggcag cgggatcgac gtcactgcgg aggacttcgc
    29461 agcggtgtcg gcccgcaaca acacgctccg gaccaacggg atcgacggca tcttcttcgg
    29521 atcgtcgact gttgggaatc agatccgaag caacagggcg tccgggaacg tcaacctcga
    29581 ctgcgaggac gactcgacgg gctcggggac ggcgggtacc gccaacacct gggccaacaa
    29641 cgacggcgtc acggacgacc cgaacgggat ctgcccgtag atcccccgac cagggggaag
    29701 ggaggccgcg gaccggaggt ccgc
//
LOCUS       DASUYD010000227         3117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_385668, whole genome shotgun sequence.
ACCESSION   DASUYD010000227 DASUYD010000000
VERSION     DASUYD010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385668"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(237..1136)
                     /locus_tag="VF195_12070"
     CDS             complement(237..1136)
                     /locus_tag="VF195_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006554928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HEX6581592.1"
                     /translation="MADEATQPAHFYGGQAVVEGVMMRGRDVWAVAVRKPAGDVHIES
                     HDIDSVAKRHPILSKPFIRGVIALGQALAIGFRALSVSARESVPEDERLSSRQMGLSM
                     GIAAVIFISLFIVLPAVAFRWVGRSVDSSILVNALEGVFRVALFLGYLLLIGRTKDIR
                     RVFQYHGAEHKTIAAYEHDEPLVPERVDGYSTLHVRCGTNFLLLVMVITIFVFALFGN
                     PGLWWRVGSRIVAIPIIAGIAFELLRLGARFPRSRIIHALMVPGLWLQKITTKPPDRG
                     QIEVAIASFNEVLRRESELTPGT"
     gene            complement(1147..1446)
                     /gene="rpmE"
                     /locus_tag="VF195_12075"
     CDS             complement(1147..1446)
                     /gene="rpmE"
                     /locus_tag="VF195_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012808210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX6581593.1"
                     /translation="MKQDIHPDYVVATVRCSCGNTFTTRSTQPELRVEICSNCHPFYT
                     GKQKLVDTGGRVERFQRRYAKQQAEQAASGRERAARPAPPASEPAEAPSVEPEQD"
     gene            complement(1516..>3117)
                     /gene="rho"
                     /locus_tag="VF195_12080"
     CDS             complement(1516..>3117)
                     /gene="rho"
                     /locus_tag="VF195_12080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEX6581594.1"
                     /translation="LEGKVAAELHQIAADLGIEGHKGLKKADLVDLIVAHTNGDTASE
                     SPPRGGASDEERSAAGDATDGNGSSGDARADTTTEISPPSPERFERRDERDGGRREGG
                     RPERGRPEGGRPDTGRSDGGQQQRDREGGPRRRPSREERRRQREERRQREVEMQKEEL
                     QTAPTATGLLDVLPEGYGFLRTSGYLPGQQDIYVSLSQVRRFMLRKGDTVTGKVRQPK
                     DSEKYFALLQIDSVNGMDPEEAKQRPNFDKLTPLFPNERFRLETGPTDVTGRIMDMVA
                     PIGKGQRGMVVSPPKAGKTTVLKNIASGIIQSSPETHVMVLLVDERPEEVTDWQRTVQ
                     AAEVVYSTFDKPPDQHIQVTELVLERAKRLAEMGQDVAIILDSITRLARAYNLAAPAS
                     GKILSGGIDSTALYPPRRFFGAARNIEEGGSVTIIASALVETGSRMDEGIFEEFKGTG
                     NMELRLDRQLAEKRLFPAINVEASSTRKEELLMPTEELALVWRLRRVLHALEPGAALE
                     LLTDKIRSTKSNEQFLREIAKAPTS"
BASE COUNT          454 a         1064 c         1045 g          554 t
ORIGIN      
        1 agctgctcca ggtaggcgcg gacctgatcc ggatcgtatc cgcgcctgat cgtgacgaat
       61 tcccgtcgcc ggatctgctc ggcggagatc agcacgggga ggtcgaggtt cgtcgcggac
      121 atggggaagc ctcctcgaac gctcggtggg gttccggagg ctacacccgt gaggagccga
      181 tgcaagcatc tccgaccatc cgaggcggag gaggtccggg ccgccggagc ccggtgtcac
      241 gtgcccggcg tcagctccga ctcgcgacgg agcacctcgt tgaacgaggc gatcgcgacc
      301 tcgatctgac cgcgatccgg tggcttcgtg gtgatcttct gcagccacag cccgggtacc
      361 atcagcgcgt ggatgatcct cgaccgcggg aacctcgccc ccagacgcag cagctcgaac
      421 gcgatccccg cgatgatggg gatcgccacg atccgcgacc cgactcgcca ccagagcccg
      481 ggattgccga acaacgcgaa gacgaagatc gtgatgacca tcacgaggag caggaagttc
      541 gtcccgcagc ggacgtgcag cgtggaatag ccgtcaaccc gctcggggac gaggggctcg
      601 tcgtgctcgt acgcggcgat cgtcttgtgc tccgcaccgt ggtactggaa gacgcggcgg
      661 atgtctttcg tacgcccgat cagcaggagg tagccgagga agagcgcgac acggaagacg
      721 ccttcgagcg cgttcaccag gatgctcgag tcgaccgacc gtccgaccca tcggaacgcg
      781 accgccggca ggacgatgaa caggctgatg aagatcacgg cggcgatccc catggacagg
      841 cccatctggc gcgacgacag gcgttcgtcc tcgggtaccg actcacgggc tgagaccgag
      901 agcgcccgga acccgatcgc gagggcctgt cccagcgcga tgaccccgcg gatgaacggc
      961 ttgctgagga tcggatggcg tttggccacc gagtcgatgt cgtgcgactc gatgtggacg
     1021 tcgccagcag gtttgcgaac cgcgaccgcc cacacgtcgc ggccacgcat catcacgcct
     1081 tcgacgacgg cctgaccgcc gtagaagtgg gccggttgcg tcgcctcgtc ggccatcctg
     1141 gccgggtcag tcctgctctg gctcgaccga tggcgcttcg gcgggctctg aagctggggg
     1201 ggcggggcgc gccgctcgtt cccggcccga cgccgcctgc tcggcctgct gcttcgcgta
     1261 gcggcgttgg aaccgctcga cccgcccgcc cgtgtcgacc agcttctgct tgcccgtgta
     1321 gaacgggtgg cagttcgagc agatctccac gcgcagctcg ggctgggtgc tccgggtcgt
     1381 gaacgtgttc ccgcaggaac agcgcacggt cgcgaccacg tagtcggggt ggatgtcctg
     1441 tttcatgatg cgcacgctct ctttcgtatc cgtctctcgt ttcgctctta tcttcgggcc
     1501 cttctccgtc ccagcttacg aggtcggggc cttggcgatc tcccgcagga actgctcgtt
     1561 ggacttggtc gaccggatct tgtcggtgag caactcgagc gcggcgcccg gctccagcgc
     1621 gtggagcacc cgccgcagcc gccagacgag ggccagctcc tcggtcggca tcagcagctc
     1681 ctccttgcgg gtgctgctcg cctccacgtt gatcgccggg aacagacgct tctcggcgag
     1741 ctgtcgatcg agtcggagct ccatgttgcc ggtgcccttg aactcctcga agatcccctc
     1801 gtccatccgc gagccggtct cgacgagggc gctcgcgatg atggtaacgc tcccgccttc
     1861 ctcgatgttc cgagcggctc cgaagaagcg tcggggcggg tacagcgccg tcgaatcgat
     1921 gccgccggac aggatcttgc ccgaagccgg tgcggccagg ttgtaggcgc gcgcaagccg
     1981 ggtgatcgag tccaggatga tcgcgacgtc ctgccccatc tcggccagtc gcttggcccg
     2041 ttccagcacc agctcggtga cctggatgtg ttggtccggc ggcttgtcga aggtcgagta
     2101 gacgacctcg gcggcctgca ccgtccgctg ccagtccgtg acctcctcgg gccgctcgtc
     2161 gacgaggagg accatcacgt gggtctcggg actcgactgg atgatgccgc tcgcgatgtt
     2221 cttcaacacg gtcgtcttcc ccgccttcgg cggcgagacc accatgccgc gctgcccctt
     2281 gccgatcggc gcgaccatgt ccatgatccg acccgttacg tccgtaggac ccgtctcgag
     2341 gcggaagcgc tcgttgggga acagcggtgt gagcttgtcg aagttcggcc gctgtttggc
     2401 ctcttccgga tccatgccgt tcacggagtc gatctgcagc aaggcgaagt acttctcgct
     2461 gtccttcggc tgccgcacct tgccggtgac cgtgtcaccc ttgcgcagca tgaagcgacg
     2521 aacctgcgag agggacacgt agatgtcctg ctgtccgggc aggtagccgg aggtgcgcag
     2581 gaacccgtag ccctcgggca ggacgtcgag cagccccgtc gccgtcggcg cggtctgcag
     2641 ctcctccttc tgcatctcga cctcgcgctg tcggcgctcc tcgcgctggc gccggcgctc
     2701 ctcccgagac gggcgcctgc gaggcccgcc ctcgcggtcg cgctgctgct gtccgccgtc
     2761 ggagcggccc gtgtccggac ggcctccctc gggacggcct cgttcgggac ggccaccctc
     2821 ccgacggccg ccgtctcgct cgtcgcggcg ttcgaaccgc tcaggactcg gtggagagat
     2881 ctccgtcgtg gtgtcggcgc gcgcgtcgcc cgacgagccg ttgccgtcgg tggcgtcacc
     2941 ggcggccgag cgttcctcgt cggaggcccc gccccgaggt ggagactcgg acgcggtgtc
     3001 gccgttggtg tgggcgacga tcaggtcgac gaggtcggcc ttcttcaatc ccttgtgccc
     3061 ctcgataccg aggtccgccg cgatctggtg tagctccgcg gcgaccttcc cttcgag
//
LOCUS       DASUYD010000228         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1708135, whole genome shotgun sequence.
ACCESSION   DASUYD010000228 DASUYD010000000
VERSION     DASUYD010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708135"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..443)
                     /locus_tag="VF195_12085"
     CDS             complement(165..443)
                     /locus_tag="VF195_12085"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX6581595.1"
                     /translation="MARAGLPRDPRFGRGLHLYVFVLRRWTASAVSYEGVLIPLVAIV
                     LAWRLQDERITWAFAAGSVLVLMGVYLGALRRPVEKQVPEAVRVPSER"
     gene            complement(623..1636)
                     /locus_tag="VF195_12090"
     CDS             complement(623..1636)
                     /locus_tag="VF195_12090"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HEX6581596.1"
                     /translation="MRRTRIVTLVALVVSVITGSIVAVPAGAHARKAPTPPMQVTLLA
                     DGLQGSVGATIGPDGALYVPEAELGQITRIDPTTGNATTFATGLPAQVIPLGGAIDVA
                     FVGSTMYVLVTVVGPDVGGNSVVGIYRKDGSGAFSVFADLGTWSIDHPPATAFDLPDG
                     VQFALQPVADGFLVSDGHHNRVLHVSSTGEITQRIQFPNIVPTGLAVTRGTVFMGEAG
                     PVPHIPATGKVVSFPSNAAGPNAHDVASGFSLLVDVEPGPDRALYALSQGDSPGDVPP
                     ASPAAPDSGELLLVNRNGSFSVLVDELDLPTSLDFVGDTAFVTTLNGEVWKITNVSKF
                     SNH"
     gene            complement(1675..1947)
                     /locus_tag="VF195_12095"
     CDS             complement(1675..1947)
                     /locus_tag="VF195_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEX6581597.1"
                     /translation="MPRYVVERTFPDGWHIQADTEGAELCLAIGERNADEGVTWLHSY
                     VSDDRTKSFCVYEAPTPEAIRRTAARNDLPVDVITQVRVLDPYFYD"
     gene            complement(2043..2207)
                     /locus_tag="VF195_12100"
     CDS             complement(2043..2207)
                     /locus_tag="VF195_12100"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX6581598.1"
                     /translation="MARGLRNAEIAERLVLAVKTVDHHVSAILQKLGVRTRGEASAEA
                     IRLGLAGQDR"
BASE COUNT          375 a          736 c          742 g          355 t
ORIGIN      
        1 ggtcgggagc tcggtctgca ggagccgccg aacgcgttcg tcgaggggag gttcctcggg
       61 ccggtatcga tcgaagctct gccaggcctc ggcgaccagc ttgaccgcgt gttcgagcac
      121 ctcttcgcgc ggggcgtcga gccgcagcgg aggttcgttc ccgatcatcg ctcggatggt
      181 acccggacgg cctcggggac ctgcttttcg accggccggc ggagcgcgcc gaggtagacg
      241 cccatcaaca ccaggaccga gcctgcggcg aacgcccacg tgatgcgttc gtcctgcagc
      301 cgccacgcca ggacgatcgc caccagcggg atcaacacgc cctcgtacga caccgcgctc
      361 gccgtccagc ggcgcagcac gaagacgtag aggtgaagac cacgaccgaa ccgaggatca
      421 cgaggtaggc ctgcgcgagc cacgtcctgg tgagctcggg aacgacgtgg gcctcgcctc
      481 caggacgccg caaactcagc tcgggcgctc gatcgacatg cgaaacgggc ggcccggatg
      541 cggccgcccg tctcgcggat cacggttcaa gagtggaaga acggccgaac cagcagcgtc
      601 cccttggtgc cggtccttca ggttagtggt tgctgaactt cgagacgttc gtgatcttcc
      661 agacctctcc gttcagggtg gtgacgaacg cggtgtcacc cacgaaatcg agcgaggttg
      721 gcaggtccag ctcgtcgacc agcacggaga acgaaccgtt ccggttgacg aggagcagct
      781 cgccgctgtc cggcgccgct ggactcgccg ggggcacgtc gcccggcgag tcaccctgcg
      841 agagcgcgta gagcgcccgg tcgggaccgg gttcgacgtc gacgaggagg ctgaaaccgg
      901 aggcgacatc gtgagcgttc ggcccggcag cgttcgacgg gaaggagacg accttccccg
      961 tcgccgggat atgtggcacg ggccctgcct cgcccatgaa caccgtgccg cgagtcaccg
     1021 ccagccccgt ggggacgatg ttcgggaact ggatcctctg cgtgatctcc cccgttgacg
     1081 atacgtggag gacccggttg tggtgtccgt ccgagacgag gaacccatcg gcgacgggct
     1141 ggagcgcgaa ttggacgccg tccggtagat cgaacgcggt cgcggggggg tggtcgattg
     1201 accaggtgcc gaggtcggcg aagaccgaga aggcccccga tccgtccttc cggtagatgc
     1261 cgacgacgct gtttcccccg acgtccggtc ccacgaccgt caccaacacg tacatggttg
     1321 atccgacgaa cgcgacgtcg atcgccccac cgagggggat cacctgagcc gggagtccag
     1381 ttgcgaacgt cgtggcgttg ccggtcgttg gatcgatgcg tgtgatctgg ccgagctctg
     1441 cctcggggac gtagagcgcg ccatctggcc cgatggttgc gccgaccgag ccttggagcc
     1501 catccgcaag taacgtgacc tgcatcggag gcgtcggggc cttccgagcg tgtgcccctg
     1561 ccggaacggc aacgatcgat ccggtgatta cactcacgac gagcgcaaca agcgtgacga
     1621 tgcgcgttct cctcacggcc ttctcctctc ggacattcga tggatggatc ctcctcagtc
     1681 gtagaagtac gggtcgagga ctcggacctg cgtgatcacg tcgacgggga ggtcgttccg
     1741 tgcagcggtc ctgcggatcg cctcgggggt cggcgcctcg tagacgcaga acgacttcgt
     1801 ccggtcgtca ctcacgtagg agtggagcca ggtgacaccc tcatcggcgt tgcgttcacc
     1861 gatcgcgagg cacagctccg cgccctccgt gtccgcctgg atgtgccagc cgtccgggaa
     1921 cgtccgctcc acgacgtacc gtggcattcg cacctccctc ccccctcgca gccctcacgt
     1981 cgaaagtagg gcgcagcacc gccgaacaca tcgggaggac tacccagatt gggagccggg
     2041 gcctaccggt cttgaccggc gagccctaga cggatagcct ccgcgctcgc ctcgccgcgc
     2101 gttcgaacgc ccagcttctg caggatcgcc gagacgtggt gatcgaccgt cttcacggcg
     2161 aggacgaggc gctcagcgat ttccgcgttg cgaaggcccc gcgccacc
//
LOCUS       DASUYD010000229         8402 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_827463, whole genome shotgun sequence.
ACCESSION   DASUYD010000229 DASUYD010000000
VERSION     DASUYD010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8402
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827463"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VF195_12105"
     CDS             <1..943
                     /locus_tag="VF195_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX6581599.1"
                     /translation="RLFEGEESVLHLIERIVGPLGLRVDQASPWVDARLPGGSRVHAI
                     VPPLSLRGPVLTIRTFAPVPLSGEDIVAQGSIGPRGLRFLAACVRGRANLVVSGGAGS
                     GKTTLLGVLSAFIPDEERLITIEDAAELRLHRSHVVSLEARPPNVEGRGQVTVRDLVR
                     NALRMRPDRIIVGEVRGGEALDMLQAMNTGHDGSLSTAHANSCRHLLWRLETMAMMSD
                     VDLPAAHIRNQVASAIDVVVQLARLRDGRRVVWEIAAVEGTHRGEPVVSPLFRFRGRV
                     GDEGGSEATGAVPRVAHVLADRGEDVGDWLFEAGPDR"
     gene            940..1740
                     /locus_tag="VF195_12110"
     CDS             940..1740
                     /locus_tag="VF195_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6581600.1"
                     /translation="MIGLAAGLLALAGFFAERAIAITRSHRSLIGLGAPLPDRASWVP
                     RRSVVLAVGFVVVMLWMGPLVGIAGAGVGALVHYSMRRHRRSGRRHRAAEQIADAVSA
                     LAAGLRAGLSLPQALAYARDEAEEPLRGELAVLVDAIATGTPVAEALTAWADTHGSED
                     ALLITGVLDLHRRSGGDLPIVLDGLASTLRERRAAHREVRALTAQARLSGVILGMLPI
                     GFFAFLFMTSRDEMLDAIGTPLGGISLGIGLVLELLAFVWIRHLLEVR"
     gene            1740..2444
                     /locus_tag="VF195_12115"
     CDS             1740..2444
                     /locus_tag="VF195_12115"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX6581601.1"
                     /translation="MEPIVAECLAAAAGACMVTGIGLALPRSRKASGKGSSVDARSAW
                     GAVAVIAAVVAPGPKIVTVPLSVLLALAVRRFAEERAGARRRRAMDAEIPQLLDLLAA
                     GSAAGLSAVAGFQRSVQALRGPLGTELKASLDAVAVGARWRTELSAVTERLALPDLRR
                     AMAVLTRTETLGTSLTGATRDLAADVRSSRRAAVAERARAAPVKMLFPLVFLVLPAFL
                     LLTVVPVLLTTVRSIR"
     gene            2459..2671
                     /locus_tag="VF195_12120"
     CDS             2459..2671
                     /locus_tag="VF195_12120"
                     /inference="COORDINATES: protein motif:HMM:NF025395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HEX6581602.1"
                     /translation="MLFLYALFLRTVDSLRLHREEGQTTAEYALVILAAAAVAVVLIA
                     WARSSGKLPAFFDSVIDSVIGDAAAA"
     gene            2655..3053
                     /locus_tag="VF195_12125"
     CDS             2655..3053
                     /locus_tag="VF195_12125"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEX6581603.1"
                     /translation="MRPLPDERGSQRGTAAVEFALVLPLVLVVALALVQMGLLVRDRL
                     LVEAAARAGARAAAVQDDPSAIRTAALAAAPSLEDGSVEVEVARAGLRGEPVTVSLRY
                     AAPVRVPFVEWLFGGPVGMSASTTARQEFG"
     gene            3050..3430
                     /locus_tag="VF195_12130"
     CDS             3050..3430
                     /locus_tag="VF195_12130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HEX6581604.1"
                     /translation="MRSGVRICERGSISIVVAAAVGMALVVTMGAADVGRALIARSRA
                     EAVADFAALAAAQELALPSGIDPSTVAAEYADRNGGRLVSCSCVPGRSEAVVEVAVPA
                     RGFLLPLEDRDVIGVARAVVDLPA"
     gene            complement(3432..3908)
                     /locus_tag="VF195_12135"
     CDS             complement(3432..3908)
                     /locus_tag="VF195_12135"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEX6581605.1"
                     /translation="MGSNPYDSLEPGTPDPIPQPSFHEVSSIGAITRGFRKRCPRCGE
                     RDAFVTWFQTRTACPRCDLRFAKEEGGFLGAMTLNYVVAIGIWLIVLVVGIILTVPDV
                     PVAALLVASAIVLIGVPLWFYPRSKMIWAAVEFLVLRGTPEYRPPVARDARQRELE"
     gene            complement(3946..5307)
                     /locus_tag="VF195_12140"
     CDS             complement(3946..5307)
                     /locus_tag="VF195_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581606.1"
                     /translation="MKYARRGTWGGILARRVRIVVGEGRTAQQGLLRFVLEGEGYEVV
                     GEAKGTSELSGVIEANRPDVVVLDDGIGVVAVSMVHDVAPQAKVVLVWPSAVVPIGGD
                     ARVEPAKILRDLGPAVERVMAPVTALTETFERPGWIDQVRKDPATLRNKLAGKETKQR
                     PSVTRLQRRSRRLHPRARQAAAAERETREEAIPAPVVVLPVGTGGADVEPVLDIGGGP
                     SVARPVEESIDERSEWNRRLGTLALSGAAAVSALVLALALGGSRVSVGGVRGSAGNVP
                     IVPGFGQGAIVGRGPLTSGVLGAGGYAPGAFDPADLVPDGGIADPTTDGGLDGSLANP
                     DGSVDPGTDPGTDPGTDPGTDPGTDPGTDPGTDPGPDPGPDPGPDPGPDPGPGDEDDD
                     DGDDDGNGDDDDDGDDDDGLPGKSAEHNPHGGPPGHLGTTPAHGPGNGVGNGQGQGHR
                     HKD"
     gene            5466..>8402
                     /locus_tag="VF195_12145"
     CDS             5466..>8402
                     /locus_tag="VF195_12145"
                     /inference="COORDINATES: protein motif:HMM:NF016944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEX6581607.1"
                     /translation="MSDTIEQSARATTPSDLHPKIEEMLARMGRGPEAGARAGTQAGA
                     EARIGTERAEAVASFARALLRRLAGDDLEAITTDELLALATSAFAFADSRGLEASAVR
                     VFNADPDVHGYRCHGTVIEVTTDDSPFLVDSVSEELSARGLTIKRLLHPVIGAVRDEQ
                     GRLIGVRSGREAEHRESFMHFEVDRALGDDVNAELEARVRRILRDVALVVRDFDPMRE
                     RVRHMIELARAAEVLYPSEVVGEVAKFLDWLLQLNFVLLGYREYELLETKGGRAIRTV
                     PGSGLGILSDVEKSTFANLTLLSDLAPDVRDRIEDGELLIYSKTQAYSTVHRRARMDY
                     IGVRTISPDGKIVGEARLIGLFTSKAYMEPAAKTPLLHHKLERLIATEDLIPGSHDYK
                     AVVALFESFPKDELFQASAEELRRLVAGLLQLEKHGGIRVLIRRDLYGRNVSVVVALP
                     RDRFNADLRKALQGLFLERFNGTTIDYHLSLGETESARIFFTIHVAPGKPIPDVRYEE
                     LEREVEILARTWEDDLLDALTRRVGAGRALALMEEYAPRFPDYYKANDEWDLVVDDVV
                     MLEKLEASTEGFLVGVGNESKGERLTRVKLYKTGGKVDLSDFMPILEALGLRVVEEVP
                     TAVAGEGRVYIHDFGVLDSRAAVLELGESADRVTDTIAAVWRGECDSDSLNRIVTLSA
                     LTWWEVRILRALRNYRMRVSARYTENYVNDAMAAYPSISERLVRMFEARFDPVRAASE
                     EEIDEIRQEIHRDLRGVSSLDQDTILRHLLGTIEAIVRTNAYVSDRSFLSFKLLSERV
                     PEMPKPMPLYEIWVYSTEMEAVHLRAGMVSRGGIRWSDRREDFRTEVLGLMKAQKVKN
                     AVIVPDGSKGGFVLKRTSVTPEQIKDEVAKQYVTFMEGLLDITDNLVKGEVVHPEGVR
                     VTDGPDPYLVVAADKGTAPLSDTANGVSERVGYWLGDAFASGGSHGYDHKELGITARG
                     VWESVKR"
BASE COUNT         1161 a         2891 c         2977 g         1373 t
ORIGIN      
        1 ccggctgttt gagggggagg agtcggtcct tcatctgatc gagcgcatcg tgggtcccct
       61 gggcctccgg gtcgaccagg cgtcaccttg ggtggacgcg cgacttccgg gcggcagcag
      121 ggtgcacgcg atcgttccgc ctctttcgct ccgcggtccc gtgctcacga tccgcacgtt
      181 cgctcccgtg cctctgtccg gtgaggacat cgtggcgcag ggcagcatcg gcccacgcgg
      241 gctgcgattc ctcgcggcat gcgtccgcgg ccgcgccaat ctcgtcgtga gcgggggggc
      301 gggtagcggc aagacgacac tcctcggcgt cctgtccgcg ttcatcccgg acgaggagcg
      361 cctcatcacg atcgaggatg cggccgagct ccgcctccat cggtcccatg tcgtgtccct
      421 cgaagcgagg ccgccgaacg tcgaggggag aggtcaggtc acggtccgcg acctcgtccg
      481 caacgcgctc cgcatgcgcc ccgaccggat catcgtgggc gaggtccggg gcggtgaggc
      541 tctcgacatg ctgcaggcga tgaacacggg gcacgacgga tcgctctcca ccgcgcacgc
      601 gaactcgtgc cggcacctgc tgtggcggct ggagacgatg gcgatgatgt cggatgtcga
      661 tctcccggcc gcccacatcc gcaatcaggt cgcctccgcg atcgatgtgg tggtgcagtt
      721 ggcgcggctc cgggacggtc ggcgcgtcgt ctgggagatc gcggccgtcg aaggcaccca
      781 tcgaggcgaa cccgtcgtct caccgttgtt ccggttccgt gggcgtgtcg gcgatgaagg
      841 agggtccgag gccaccggcg ccgtgccacg ggtggcccac gtcctggccg atcgaggcga
      901 ggacgtcggc gactggctgt tcgaggcggg gccggaccga tgatcgggct cgccgccggt
      961 ctcctggcgc tcgccggctt cttcgcggag cgcgccatcg cgatcacccg ctcccaccga
     1021 tcgctcatcg gcctgggtgc gccgctaccg gatcgggctt cgtgggtgcc tcggcgatcg
     1081 gtcgtgctcg ccgtcgggtt cgtggtggtg atgctctgga tgggcccctt ggtcgggatc
     1141 gcgggggcgg gggttggagc gctcgttcac tactccatgc ggcgacatcg acggtcggga
     1201 cggcggcacc gggcggcaga gcagatcgcc gatgcggtga gcgcattggc cgccggcctc
     1261 cgcgccgggc tgtcccttcc acaggccctc gcctacgcgc gggacgaggc ggaggagccg
     1321 ctccggggtg agctcgcggt cctcgtcgac gcgatcgcga ccggcacgcc cgtcgcggag
     1381 gcgctgaccg cgtgggcgga cacgcacggg tccgaggatg cgctcctgat caccggggtc
     1441 ctcgacctcc atcgccggag cggtggcgac ctcccgatcg tgctcgacgg tctcgcgagt
     1501 acgctccgcg agcgccgtgc ggcacatcga gaggtccggg ccctcacggc gcaagcgcgc
     1561 ctgtccgggg tgatcctcgg catgcttccg atcggcttct tcgcgttcct ctttatgacc
     1621 tcccgagacg agatgctcga cgcgatcgga accccgctcg gaggcatctc gctcggcatc
     1681 ggcctggtgc tcgagctcct ggcgttcgtc tggatccgac acctgctcga ggttcgttga
     1741 tggagccgat cgtcgcggag tgcttggcgg ccgcagctgg agcgtgcatg gtgaccggga
     1801 tcgggctcgc cctgccccgg agccgcaagg cgtccgggaa ggggtcatcg gtcgacgccc
     1861 gctccgcctg gggagcggtc gccgtgatcg ccgcggtggt cgctcccggt cccaagatcg
     1921 tgaccgtccc actgtcggtc ctgctcgcgc tcgcggtccg caggttcgcc gaagagcgag
     1981 cgggggctcg ccgtcgccgc gccatggacg ccgagatccc ccagctgctc gatctcctgg
     2041 cagccggatc ggccgcgggc ctgtcggccg ttgccggatt ccaacggtcg gtgcaggcac
     2101 tccgaggtcc gctcgggacg gagctcaagg cctcgctcga cgccgtggcg gtcggcgcca
     2161 gatggcggac ggagctttcg gcggtcaccg agaggctcgc acttccggat cttcgtcggg
     2221 cgatggccgt gctgacccgc accgagacac tgggaacatc cctcactggc gcgacgcggg
     2281 acctcgccgc ggacgtccgc agctctcggc gtgcggcagt cgcggagcgg gcccgcgccg
     2341 cgccggtgaa gatgctcttc ccgctcgtgt tcctggtcct ccccgctttc ctcctcctca
     2401 cggtggttcc cgtcctgctc accaccgtgc gttccatccg atgagaggag gtgaaccgat
     2461 gctcttcctg tacgccttgt tcctgcgcac cgtcgactcc cttcgcctcc acagggagga
     2521 aggtcagacg acggccgaat acgcgctcgt gatcctggct gccgcggcgg ttgccgttgt
     2581 cctgatcgct tgggcccggt cgtccggcaa gctgccggcg ttcttcgaca gcgtgatcga
     2641 cagcgtgatc ggcgatgcgg ccgctgcctg acgaacgcgg gtcgcaacgg gggaccgcgg
     2701 ccgtcgagtt cgctctcgtc ctcccgctgg ttctggtggt ggcgctcgcg ctcgtgcaga
     2761 tgggactgct cgtccgcgat cgtctgctcg tggaggcggc ggcgcgcgcg ggtgcgcgcg
     2821 ccgccgccgt ccaggacgat ccgtccgcca tccgaacggc cgcccttgcc gccgcgccgt
     2881 cgctcgagga tgggtccgtc gaagttgagg tcgcacgagc gggcctccgg ggcgagccgg
     2941 tgaccgtctc gctgcggtac gcggcccccg tgcgcgtgcc gttcgtggag tggctgttcg
     3001 gcggtccggt gggcatgagc gcctccacga ccgccaggca ggagttcggg tgagatccgg
     3061 cgtccggatc tgcgagcgag gaagcatctc gatcgtcgtc gctgcggcgg ttgggatggc
     3121 cctcgtcgtc acgatgggcg cggccgatgt cggcagggcg ctgatcgcca ggtcgcgcgc
     3181 cgaggcggtg gcggacttcg cagcgctggc cgccgcgcag gagctggcgc ttccttccgg
     3241 catcgatccc tcgaccgtcg cggccgagta cgcggatcgc aacggcggcc ggctcgtctc
     3301 gtgctcctgc gtcccgggcc ggtccgaagc ggtcgtggaa gtcgcggtgc cggcacgcgg
     3361 gttcctcctt ccgctcgaag accgggacgt catcggcgtc gcgcgggcgg tggtggatct
     3421 cccggcctga gctactcgag ctctcgctgg cgtgcatcgc gcgcgaccgg cggccggtac
     3481 tcgggcgtgc cgcgcagcac caggaactcg acggcggccc agatcatctt cgaccgaggg
     3541 tagaaccaga gtgggacgcc gatcagcacg atcgcgctcg ccaccagcag ggccgcgacg
     3601 gggacatcgg gaaccgtcag gatgatcccc acgacgagga cgatgagcca gatgccgatc
     3661 gcgacgacgt agttcagggt catcgcgccg aggaacccgc cctcctcctt cgcgaaccgc
     3721 agatcgcaac gcgggcacgc ggtccgcgtc tggaaccagg tcacgaacgc atcccgttca
     3781 ccgcagcgag ggcagcgctt ccggaagccg cgagtgatcg ccccgatgga gctcacctcg
     3841 tggaacgaag gctgcgggat cggatccggt gtgccgggtt cgagcgagtc gtaagggttg
     3901 gaacccatag cctcagccta ggcttctgga tcgaggctcc ctcggtcagt ccttgtgcct
     3961 gtgcccctgg ccctgcccgt tgcccacgcc attgccgggg ccgtgagcgg gcgtggtccc
     4021 caggtggccg ggggggccac cgtgcggatt gtgctccgcg ctcttccctg ggaggccgtc
     4081 gtcgtcgtcc ccgtcgtcgt cgtcgtcccc gttcccgtcg tcgtccccgt cgtcgtcgtc
     4141 ctcgtcgccg ggtccggggt cgggtccggg gtcgggcccg gggtcgggtc cggggtcggg
     4201 tccggggtcg gttccggggt cggttccggg gtcggtcccg gggtcggttc cggggtcggt
     4261 cccggggtcg gtcccggggt cggttccggg atccacgctg ccgtcggggt tcgcgaggct
     4321 gccatcgagg ccaccgtcgg tcgttgggtc tgcgatgccg ccgtccggta cgaggtcggc
     4381 ggggtcgaac gcgccgggtg cgtagccacc tgctccgagc acgcccgagg tgagcggccc
     4441 gcgaccgacg atcgcaccct gcccgaagcc ggggacgatg gggacgttgc cggccgagcc
     4501 gcggacccca ccgacggaca ccctgctgcc gccgagggcg agcgcgagca ccagagcgct
     4561 caccgccgcc gcgccgctca gcgcgagggt gccgaggcgc cggttccact ccgaccgctc
     4621 gtcgatcgac tcctcgaccg gacgagcgac ggacgggcct ccgccgatgt cgagcacggg
     4681 ctcgacgtct gctccaccgg taccgacggg gagcaccacg accggcgccg ggatcgcctc
     4741 ctcgcgcgtc tcgcgctctg ctgcggccgc ctgtcgggcc ctggggtgga ggcgccggct
     4801 gcgccgctgc aggcgggtga cgctggggcg ttgcttcgtc tccttgccgg cgagcttgtt
     4861 ccgcagcgtg gccgggtcct tgcggacctg atcgatccag ccgggccgct cgaacgtctc
     4921 agtgagcgcc gtgaccggtg ccatcacgcg ctccaccgcc gggccgaggt cccggaggat
     4981 cttggccggc tccacgcggg catcgccgcc gatcgggacg acggccgacg gccacacgag
     5041 gaccaccttc gcctgggggg cgacgtcgtg gaccatggac acggcgacca cgccgatgcc
     5101 gtcgtccaaa acgaccacat ccggacggtt ggcctcgatc acgccggaga gctcggaggt
     5161 gcccttggcc tcacccacga cttcgtagcc ctcgccctcg aggacgaagc gaaggagccc
     5221 ttgttgcgcc gttcgaccct cgccgacgac gatgcgtacc cgtcgcgcca atatgcctcc
     5281 ccaggtgccc cgccgagcgt acttcacgca cgggttctcg gcctcagcct tctgggggtt
     5341 ttcgtgccga aaggagtagt acagcccgta cgaccttcgg taccggccgt cgcgctcggt
     5401 gtccccatct cggtccgatg acctgacaca atggagcggt cagccccgga gggtcgcgaa
     5461 cgccagtgtc ggacacgatc gaacagtcag cgcgcgccac caccccctcc gacctccatc
     5521 ccaagatcga ggagatgctg gctcggatgg ggcgcggccc cgaggccggt gcgcgggccg
     5581 ggacgcaggc cggcgccgag gcacggatcg ggaccgagcg ggccgaggcg gtcgcctctt
     5641 tcgcccgagc gctcctgcga cgcctggcgg gcgacgacct cgaggcgatc acgaccgacg
     5701 agctgctcgc gctcgccaca tccgcgttcg cgttcgcgga cagccgcggc ctcgaggcgt
     5761 ccgccgtccg cgtcttcaac gccgaccccg acgtccacgg gtaccgatgc cacggtacgg
     5821 tcatcgaggt cacgaccgac gactcgccct tcctcgtcga ctcggtctcg gaggagctca
     5881 gtgcgcgcgg tctcacgatc aagcgtctgc tccaccccgt gatcggcgcg gtccgcgacg
     5941 agcaggggcg gctgatcggc gtccggtccg gacgggaagc cgagcatcgc gagtcgttca
     6001 tgcacttcga ggtcgaccgc gctctcggcg acgacgtgaa cgcggagctc gaggcgcgcg
     6061 tgcgccgcat cctccgagat gtcgcgctcg tggtccggga cttcgatccg atgagggagc
     6121 gcgtgcggca catgatcgag ctcgcacgcg ccgccgaggt cctctatccg tcagaggtcg
     6181 tcggcgaagt ggcgaagttc ctcgactggc tcctccagct gaacttcgtt ctgctcggct
     6241 accgcgagta cgagcttctg gagacgaagg gcggacgcgc gatccgtacg gtgccgggca
     6301 gcgggctcgg gatcctctcg gacgtcgaga agtcgacgtt cgcgaacctg accctgctct
     6361 cggacctcgc accggatgtc cgggatcgga tcgaggacgg ggagctcctg atctactcga
     6421 agacccaggc gtactcgacc gtccaccgtc gcgcccgcat ggactacatc ggggtccgga
     6481 cgatctcacc cgacgggaag atcgtcggtg aggcccggct gatcggcctc ttcacaagca
     6541 aggcgtacat ggagccggcc gcgaagacgc cgttgctgca ccacaagctc gagcgtttga
     6601 tcgccacgga ggatctgatc cccgggtcgc acgactacaa ggcggtggtc gccctcttcg
     6661 agtcgttccc caaggacgag ctcttccagg cgtccgccga ggagctgcga cggctggtgg
     6721 ccgggctcct gcagctcgag aagcacgggg ggatccgcgt cctgatccgc cgcgacctct
     6781 acggccgcaa cgtgtccgtc gtggtcgccc tgccgcgtga tcggttcaac gcggaccttc
     6841 ggaaagccct ccagggcctc ttcctcgagc gcttcaatgg cacgacgatc gactaccacc
     6901 tctccctggg ggagacggag tcggcgcgga tcttcttcac gatccacgtc gcaccgggca
     6961 agccgatccc agacgtgcgc tacgaagagc tcgagcggga ggtcgagatc ctggcgcgca
     7021 cctgggagga cgacctcctc gacgcgctca cgcggcgcgt cggcgcgggc cgcgcgctcg
     7081 ccctgatgga ggagtacgcg cctcggttcc cggactacta caaggccaac gacgagtggg
     7141 atctggtcgt cgacgacgtc gtcatgctcg agaagctcga ggcgtcgacc gagggcttcc
     7201 tcgtcggggt cggcaacgag tccaaaggcg aacgcctgac gcgggtcaag ctctacaaga
     7261 caggtggcaa ggtcgatctc tcggacttca tgccgatcct cgaagcgctc gggctgcggg
     7321 tcgtcgagga ggtgccgacc gcggtcgcag gcgagggacg ggtgtacatc cacgacttcg
     7381 gggtcctcga ctcacgggcc gcggtgctgg agctcggcga gtcggcagat cgcgtcacgg
     7441 acacgatcgc ggccgtgtgg cgaggggagt gcgactccga ctcgctgaac cgcatcgtga
     7501 cgttgtcggc cctcacctgg tgggaggtca ggatcctccg cgccctcagg aactaccgca
     7561 tgcgcgtctc cgcccggtac acggagaact acgtgaacga cgcgatggcg gcctacccgt
     7621 cgatctcgga acggctcgtg cggatgttcg aagcccggtt cgatcccgtg cgtgccgctt
     7681 cggaagagga gatcgatgag atccggcagg agatccaccg ggacctgcga ggcgtctcct
     7741 ccctcgacca ggacaccatc ctgcgacacc tgctcgggac gatcgaggcg atcgtgcgga
     7801 cgaatgcgta cgtctccgac cgttcgttcc tcagcttcaa gctcctgtcg gagcgtgtgc
     7861 cggagatgcc gaagcccatg ccgctctacg agatctgggt gtactccacg gagatggagg
     7921 cggttcacct tcgggccggc atggtctccc gcggggggat ccgatggtcc gatcgcagag
     7981 aggacttccg caccgaggtc ctcggactga tgaaggccca gaaggtcaag aacgccgtga
     8041 tcgttcccga cggctccaaa ggtgggttcg tgctcaagcg aacctcggtc acgccggagc
     8101 agatcaagga cgaggtggcg aagcagtacg tcacgttcat ggagggactg ctcgacatca
     8161 ccgacaacct cgtgaaggga gaagtcgtgc atcccgaggg tgtgcgcgtg acggacggcc
     8221 ccgatcccta cctggtggtc gccgccgaca agggcacggc tcccctgtcg gacacggcga
     8281 acggcgtgag cgagcgggtc gggtactggc tcggtgacgc gttcgcctcc ggaggctccc
     8341 acggctacga ccacaaggag ctcggcatca ccgctcgcgg cgtgtgggaa tcggtcaagc
     8401 gg
//
LOCUS       DASUYD010000230         9815 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1493339, whole genome shotgun sequence.
ACCESSION   DASUYD010000230 DASUYD010000000
VERSION     DASUYD010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493339"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..866
                     /locus_tag="VF195_12150"
     CDS             <1..866
                     /locus_tag="VF195_12150"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6581608.1"
                     /translation="FHGLHGLGTELAGIRPLREGFGERYLSDFHPLPVNDSQALENRI
                     LELGADRVAAFFAEPVIGAGGVIPPAVGYLEAVQRICREHDVLFVVDEVITGFGRLGE
                     WFGSSIYGLAPDLVCFAKGVTSGYVPLGGVIASKRVWEPFAKDGSSPTFRHGYTYAGH
                     PTACAVGLANLEILEREELVWRAAEMATPFADAVSTLGTHTTVKEVRTSGMMAAVQVD
                     TDGVMGEDLPSRAAQRARELGVLTRVLFGGSLQISPPLVIEPSEIQRIVETLGQALTE
                     CAADPTTGRRT"
     gene            1004..1594
                     /locus_tag="VF195_12155"
     CDS             1004..1594
                     /locus_tag="VF195_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEX6581609.1"
                     /translation="MSAVAVPTNRPKGFAYVNGEIMPTDEARVSVLDRGFLWGDGVYE
                     ITPCFNGKLFRLDQHLERLGSSLRFAGITLKIPHDDLFTSTSEVVHLNREVTSSHPVC
                     RLGHWVTRGIEHWVPLDQRDDGPTLCILVEPVGRTLTPEDYLTGVPLTIVSTRRSPSS
                     VLDPRAKSTGTSRESRHRKADRPDGPPGEAAHQSCT"
     gene            complement(1902..2024)
                     /locus_tag="VF195_12160"
     CDS             complement(1902..2024)
                     /locus_tag="VF195_12160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581610.1"
                     /translation="MKKALIVIGSLAVAVAVAAKIIHDRLARHRDDVGTAATAA"
     gene            complement(2063..2275)
                     /locus_tag="VF195_12165"
     CDS             complement(2063..2275)
                     /locus_tag="VF195_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581611.1"
                     /translation="MTETRESNLGGPLFVAGAVIMVVGLLGIYDTGGVDWVSAFIGFV
                     GAVISVAGLYLVSRRNGTRGTRHSTS"
     gene            2806..3186
                     /locus_tag="VF195_12170"
     CDS             2806..3186
                     /locus_tag="VF195_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX6581612.1"
                     /translation="MQPVGSQILRGANDPPIEFVARGRRPHPATVAYAREKVWAAARA
                     APRNVRFARITLAVEEHRSIERPARAEVALDVEGRIVRAEAAAGSPREAIDLVEQRLR
                     RRLVETPRRRSRRATGVRGRGRQG"
     gene            3429..4382
                     /locus_tag="VF195_12175"
     CDS             3429..4382
                     /locus_tag="VF195_12175"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581613.1"
                     /translation="MLVPAANHLDDPLFWQRSSKGLAIFLEPRWSRTYRLPIEVPSRA
                     TVARRFQVRPLFPLLSGDGRFYVLALSQNAIRLLIGTRHDVHGVDLARMPRGLRDALR
                     YDDPEKERLYHVAGRQGVPVPIFHGRGIGSEVDKERISRYLHLVEAGLIDVLRDERAP
                     LVLAGTDYERAIFRAITRYPALVEEGIPGSPDRVRAEALHARAWPLVEPIFQQARHEA
                     AAKYRQLEGTGKTSADLAEIVRAADRGQVDVLFVLVDEERWGTFDHTTGRVTTSEVRG
                     PGDEDLLELAAVGTFLSRGIVYAVPSHGMPDGTASAAILRF"
     gene            4440..4649
                     /locus_tag="VF195_12180"
     CDS             4440..4649
                     /locus_tag="VF195_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1918 domain-containing protein"
                     /protein_id="HEX6581614.1"
                     /translation="MKAKVGDKLVVVGHHVGEHGREAEILEVHGADGAPPYLVRWTED
                     HHQTVVFPGSDASIEHVPRHEVHSN"
     gene            5255..5394
                     /locus_tag="VF195_12185"
                     /pseudo
     CDS             5255..5394
                     /locus_tag="VF195_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007298842.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
     gene            complement(5488..7938)
                     /locus_tag="VF195_12190"
     CDS             complement(5488..7938)
                     /locus_tag="VF195_12190"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX6581615.1"
                     /translation="MIRRVAFWLRWSRRDLRARWLQVAAIALIVAIGSGMFAALGSLA
                     TWRRISYDESYSLLNAHDVHVSLPAGSWVPSGTLVEAIAHLRSQGVVTAAEERVVLPT
                     QVNASFGGRTILVPGVVIGLDLGARGPHLNKIFVTGGRALRPADAASPGAAVLEAHFA
                     DHYELPPSGEIRMSGGQALPYVGTGYAPEYFMVTTEAGGFLAESNFAAVFVPLEVAQA
                     IVGEPVVNDLVLEVALGVDRAHVVSDVSDAIRASVPGIALTVTPIDDDPAYRALYGDL
                     DNDQRTMNAVAALILAASVFAAFNLTSRVVEAQRREIGIGMALGERTSAIATRPLLMG
                     LEIGLLGAVFGIGVGLLIDEGLRAVLESMQPMPVWETTFQAVEFAKAAALGLILPLLA
                     TVWPVWRAVRVTPVEAIRTGHLAARGGRIASVMKRVTGGRRSLRVMPLRNVLRSPRRT
                     LLTAVGIAASITAMVGVIGMVDSFDATIDRGAAEIERGAPGRLEIALTTMEPIDGTTV
                     RAIEAARGVAATQPSLRLAGRLGGGSEAFDVILEVLDLDGAVWNPTIEDRISSGAVPG
                     IVLARKAARDLAVLPGDLVTVTHPLVDPENQTFLNIATRMRVLGVHPNPMRSLAFVDI
                     ADADMFGLVGMANTVHVAPAEGATAAGITRELFGLRGVASVQPANATVALLRDLMVRF
                     LDILRFVEIFVLVLALLIAFNAASIAVDERAREHATMSAFGVRTRTIIAGLTEEGIVV
                     GVLGTAIGVAFGSAAVRWLMAGTGQQMPDLDLISTVTVPTIATAFMLGVAAVGLAPLF
                     SVRRLVRMDIPSTLRVME"
     gene            complement(7946..8713)
                     /locus_tag="VF195_12195"
     CDS             complement(7946..8713)
                     /locus_tag="VF195_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011754440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581616.1"
                     /translation="MAWTKITDSGYLSHPGHREGAATAILLEQITKRYELGAVCVTAL
                     DRVDLEVDAGEFVVVLGPSGSGKTTLLNVIGSLDSPTSGRVIIAGRSIGEVGRRALTE
                     FRRTTVSFIFQSFNLFPGLTALENVQFGVDVSGARGVDAAAVLEDVGLVERIDHFPHE
                     LSGGEQQRVAIARALATGNPVMLADEPTGELDFRTGVQILDLLRSQAEAGKTVVVVTH
                     NREISRIADRVIELSSGGKVGDGPPRGGTTRIEDLHW"
BASE COUNT         1650 a         3329 c         3218 g         1618 t
ORIGIN      
        1 cctttcacgg cctgcacggg ctcgggacag aactcgctgg cataagaccc ctgcgggagg
       61 gattcggcga acgatacctc agtgacttcc accccctccc cgtcaacgat tcgcaagcac
      121 tcgagaaccg catcctggag ctcggagcgg accgcgtcgc ggccttcttc gctgagccgg
      181 tgatcggtgc cgggggggtg attccgccag cggtcggtta cctcgaggca gtgcaacgga
      241 tctgtcgaga gcacgacgtg ctctttgtcg tcgatgaagt gatcaccggc ttcggtcgcc
      301 taggcgagtg gttcggatcg tccatatacg gcctcgcgcc cgaccttgtt tgtttcgcaa
      361 aaggggtgac atccggctac gttccgctcg gaggcgtcat cgcctcgaag cgcgtatggg
      421 aaccgttcgc caaggatgga tcctctccga cattccggca cggctacaca tatgcgggac
      481 acccgaccgc atgcgcggtt ggcctcgcga acctggagat cctcgagcgg gaggagctcg
      541 tctggcgtgc tgcggagatg gcgaccccgt tcgctgacgc ggtgtcaacg ctcggcaccc
      601 ataccaccgt gaaggaggtc cggacctcgg ggatgatggc cgcggtgcaa gtggataccg
      661 acggcgttat gggcgaggat ctgccgagcc gtgcagcgca acgggctcga gagttgggcg
      721 tgttgacccg cgtgttgttc ggggggagcc tgcagatctc tccaccgctc gtgatcgaac
      781 cgagcgagat ccagcggatc gtcgagacac tcggccaggc cctgaccgag tgtgcggcag
      841 acccaaccac cgggcggcgg acctgatgcg cgtgatggcg gccggtcgcc gccgctggcg
      901 aacgctcgat cgcgatcacg caccgaaccc gacctccgga aggcgtatgc ctgttcgaga
      961 tgttggcctc gggtggcctg cctcagcgac gtgaagatcg tcgatgtcag ccgttgccgt
     1021 gccgactaac cgcccgaagg ggttcgccta cgtgaacggc gagatcatgc cgaccgatga
     1081 agcccgtgtg tccgtcctcg accgcggctt cctttggggc gatggcgtgt acgagatcac
     1141 gccctgcttc aacgggaagc tgttccgact cgatcagcac ctcgagcgcc tgggctcctc
     1201 gctgcggttc gccgggatca cgctcaagat cccgcacgac gatctcttca cgagcacctc
     1261 cgaagtcgtc cacttgaacc gggaggtgac gtcatctcat ccggtttgcc gcctcggaca
     1321 ctgggtgact cgtgggatcg aacattgggt tccgctcgat cagcgagacg acggccccac
     1381 actgtgcatc ctggtggagc cggtcggccg aacgctcacg cctgaggatt acctgaccgg
     1441 cgtgccgctg acgatcgtgt ccactcgacg atcaccgtcg tcggtcctcg acccacgagc
     1501 gaagagcacc ggcacgagcc gtgagagccg gcatcgaaag gccgaccggc ccgacgggcc
     1561 tccaggggaa gcagcgcacc agtcctgcac atgaagccgg ttcacgcgtc cccggcgacc
     1621 gctacggcga gcgggctggc agatcggggc aagcttctca agcgtcctca tgtgcgaccg
     1681 agggcttcga cgacgatggc gtctacgagg agtcggtttg agagctggcc gatcgagaac
     1741 agggacgccg gtgtcgaacg gcactcgaag gaatggccat ctgcatcagt actatagagg
     1801 acccagggcg acctctcgtt ggtcggcaac ttctcctgga cctggcgccg atccgaccac
     1861 acggctccag cccgccagtg ggctgtagct cgatcccggg cctatgcggc ggtcgcagcc
     1921 gttccgacat catcgcggtg cctggcgagt ctgtcatgga tgatcttcgc ggccactgcg
     1981 acggccacgg caagcgaccc gatgacaata agtgccttct tcatcgctca cagctctcct
     2041 cccacctcga aacggcatcg gctcacgatg tcgagtgacg cgttccgcgt gtcccgttcc
     2101 tccgggagac gaggtacaga ccagcgaccg atatcaccgc accaacgaat ccgatgaagg
     2161 cggataccca atccacccct cccgtgtcgt agataccgag cagcccaacc accatgatca
     2221 ctgctcctgc gacgaagagc ggcccgccga gattcgactc tctggtctcc gtcatcgctg
     2281 gtcacctcct cgtcatgagc actccctgtt gacttgttat cacttcgatg gaacgcctca
     2341 ggggggcgaa agtcccgcca gagtggggcc tatcaccggg gcttctcggc acgccagatc
     2401 ttcgcccacc tctcgacctc gcgccgatcc cacacacggc tccgtccctc gggtaccgac
     2461 cggagcggga aaggcgcgct cgccggcgat cttgctggcc cgctggtggc tcacgccgag
     2521 gaggtcggcg atctcgatga tgcggacagt ggccttcccc aaggctcgac actactcagg
     2581 aggggcggga gttgtagcca gagcagttgg ttgggcggtc cgttcctcgc gccgttcgtc
     2641 acaatcatct gggctcgcca cccagtcggc ttcgtgccct ctttcatccg agcctggcgc
     2701 agccgcgatc ccgcgcccag ggccgaacgt ccctgtcgtc cggggccatt ccgacctgcg
     2761 ttcgccctgg cgtcgatgac aggctgctct caggaggagc ggacgatgca gccggtcggt
     2821 tcccagatcc tgcgaggtgc gaacgaccca ccgatcgagt tcgtcgcccg aggacggcgc
     2881 ccccatccgg cgacggtcgc ttacgctcgg gagaaggtct gggcggcggc ccgcgcggcg
     2941 ccgcggaacg tcaggttcgc gcggatcacg ctcgcggtcg aggagcatcg ctcgatcgaa
     3001 cgaccagcga gagccgaggt ggcgctggac gtcgagggtc gcatcgtccg tgcggaggcg
     3061 gctgccgggt cgccgcggga agcgatcgat ctcgtcgagc agcgccttcg acgccgcctc
     3121 gtcgagaccc ctcggaggcg ttctcggagg gcgaccggcg tgaggggacg agggaggcag
     3181 gggtgagagt acacgcgaag gaaccgcagc ccgatgtgca tgagaggact ggcatctccg
     3241 atctgctcgg acggcacgac atccaggcac tcacggagcc tcgagacggt ccctgcgttt
     3301 cgctgttcct ccccacgcat ggcggaggga tgggcactaa ccaggaccgc atccgtctcg
     3361 agaacctgct cagggaaacg agggcgggtc tcgtttcgag tggcctccga gacccggacg
     3421 ctcgtcggat gctcgtcccc gcggcgaatc atctggatga ccccctgttc tggcaacgca
     3481 gctcgaaggg gctcgcgatc ttcctggaac cgcgctggtc gcgcacgtac cggttgccga
     3541 tcgaggttcc gagccgcgcc accgtggcac ggcgcttcca ggtccgtcct ctgttcccgt
     3601 tgctctcggg cgatggacgc ttctatgtac tcgccttgag ccagaacgcc atccggctgc
     3661 tgatcgggac ccgccacgac gttcacgggg tcgatctcgc gaggatgcca cgaggactcc
     3721 gtgacgccct tcggtacgac gacccggaga aggagcggct ctaccacgtc gccggtcgac
     3781 aaggagttcc ggtaccgatc tttcacggtc gcgggatcgg tagcgaggtc gacaaggagc
     3841 ggatctcccg gtatctgcat ctcgtggagg ccggcttgat cgacgtgctc cgcgacgagc
     3901 gagcgccgct cgtcctcgca ggtacggact acgagcgtgc gatctttcga gcgatcacga
     3961 gatacccggc gctggtcgag gaagggatac ccggaagccc ggatcgcgtt cgcgcggagg
     4021 cgctccacgc ccgggcctgg ccactggtcg agccgatctt ccagcaggcc cgtcacgagg
     4081 cggcggcgaa gtaccggcag ctcgaaggca ctgggaagac cagcgccgac ctcgcggaga
     4141 tcgttcgagc agccgatcgc ggacaggttg acgtgctgtt cgtcttggtg gacgaggaaa
     4201 ggtgggggac gttcgaccat acgacgggtc gggtcacaac gagtgaggta cgcggtcccg
     4261 gagacgagga tctcctcgag ctcgccgccg tggggacctt cctgagccgc ggcatcgtct
     4321 atgcggttcc ttcccacggc atgcccgacg ggacagcttc cgccgcaatc ctccgcttct
     4381 gagcagtgag ggagacgaac gatcgtgtat gtcgaccacg agactggaag gggaagacga
     4441 tgaaggcgaa ggtgggtgac aagctggtcg tcgtgggaca ccacgttggc gagcatggtc
     4501 gtgaggctga gatcctcgag gtccacggcg cggacggcgc gcctccgtac ttggttcgtt
     4561 ggaccgagga ccatcatcaa acagtcgtgt tcccgggttc ggatgcgagc atcgaacacg
     4621 tcccacgaca cgaggtccat tcgaactagt cggcgtccac cggagcggat agggaacgcg
     4681 cggccggatc agccgcgggg tcaggttcgg cagttctcga cgtcgagcca tcctttgtcg
     4741 ttcgctcgga aatccgcgcc gaacacccga acgcggaccc aagcgcatct gcgtgctcac
     4801 acggccagaa tgggcgcctg gtcgcgcgtc ttgaagggcg gttcggcccg gcatcggcgg
     4861 gccggtccgc cccgggctgg ccctcgctcg gcggatccgc gcagttggtt gcgggccagc
     4921 cagcatcggg agcggctggg tcgcgccgag caccatctgc acgatgacgt gggccggtcc
     4981 ccccgcccac ctcccgaacc aaggcggggt gcgtcacagc cgtcctgcgc ggcgcgacct
     5041 cgacctcgaa ggtttggttc cgtgctcatg gggagtctcg gggtgtgcct gcggcctccc
     5101 tggttcccat gctcgcgaga atactcctga ccgggctcct cgtcggcctg gctctttccc
     5161 tcgccttctt cccggtcgcc cgtggctgtg aaacgagcag aacgttgcct tcgcggcgtc
     5221 cttctcgctc ctcgcgcctc ctcgacggcc acgcctcgtg gccattgtgc ttccatggtt
     5281 cctcacccga ctcggaaagg atccggcctt cggcagcggt cctctcgcca cgtgatccag
     5341 gacttgctct cgatcctgat ctacttcgcg accgcctccg ccatcgtcga ctgagtcctg
     5401 gggccggtac ggggagtgtc caatcgaccg ccgcgccggc aagggatgac ggcgtggcct
     5461 cctcgagcct ctccgccggc acgcccgtca ctccataact cggagggtcg aagggatgtc
     5521 catccggacc aaacgtcgga cggagaacag cggcgcaagc ccgaccgccg cgactccgag
     5581 catgaacgcg gtcgcgatgg tgggtaccgt gacggtcgag atcagatcga ggtcgggcat
     5641 ctgctgacct gtacccgcca tgagccaccg aacggcggcg gagccgaagg ccacgccgat
     5701 ggcggtacct aggacgccca ccacaatgcc ttcctcggtg aggccggcga tgatcgtgcg
     5761 cgtccgcacg ccgaacgcag acatcgttgc gtgctcgcgc gcgcgttcgt cgactgcgat
     5821 gctcgcagcg ttgaaagcga tcagcagcgc gagcacgagg acgaagatct ccacgaaccg
     5881 caggatgtcg aggaaccgca ccatcagatc gcgcaggaga gcgaccgtcg cgttcgccgg
     5941 ttggaccgag gcgacacccc ggaggccgaa cagttcgcgg gtgattccgg cggcggttgc
     6001 accctctgcg ggcgcgacgt gcacggtgtt ggccatcccg accagaccga acatgtccgc
     6061 gtcggcgatg tcgacgaagg caagcgaccg catcgggttg ggatgcacgc cgagcacgcg
     6121 catccgcgtc gcgatattca ggaaggtctg gttctcgggg tccacgagcg ggtgggtcac
     6181 cgtcaccagg tcgcctggaa ggacggccag gtcgcgggca gccttgcgcg cgagcacgat
     6241 ccccggcacc gctccggaac tgatccgatc ctcgatcgtc gggttccata cagcgccgtc
     6301 caggtcgaga acctcgagga tcacgtcgaa cgcctcggat ccgcccccga gccggcctgc
     6361 gagccgcaac gacggttgcg tcgccgcgac gcccctcgcg gcctcgatcg cccggaccgt
     6421 cgttccgtcg atcggctcca tcgtggtcag ggcaatctcg agacgccctg gcgcgcctcg
     6481 ctcgatctcg gcggcacctc gatcgatcgt ggcgtcgaac gagtcgacca tgccaatcac
     6541 accgaccatc gcggtgatcg acgcagcgat gccgaccgcc gtgaggagcg ttcgccgcgg
     6601 cgagcgaagg acgttccgca acggcatcac gcgaaggctc cgccgcccgc cggtgacccg
     6661 cttcatcacc gacgcgatgc gcccgcccct cgccgccagg tgaccggttc ggatcgcctc
     6721 gactggcgtg acgcgcacgg cccgccacac cggccacacc gtagcgagga gcggaaggat
     6781 caggccgagc gccgcggcct tcgcgaactc gaccgcctgg aaggtcgttt cccagaccgg
     6841 catcggctgc atcgactcga gcaccgccct gaggccttcg tcgatcagca ggcccacgcc
     6901 gatgccgaac accgccccga gcagcccaat ctcgagtccc atcagcagcg gccgggtcgc
     6961 gatcgccgac gtgcgctcgc cgagcgccat cccgatcccg atctcccgac gctgcgcctc
     7021 cactacgcgg ctcgtgaggt tgaacgccgc gaacacggac gccgcgagga tcagcgctgc
     7081 caccgcgttc atcgtgcgct ggtcgttgtc gaggtcgccg tacagagctc ggtacgcggg
     7141 atcgtcgtcg atcggggtca ccgtgagcgc gatgccgggg acactcgcgc ggatggcgtc
     7201 gctcacgtca gatacgacat gagcgcggtc tacgccgagt gcgacctcga ggaccaggtc
     7261 gttcaccacc ggctcgccga cgatcgcctg cgcgacctcg agcggcacaa acaccgccgc
     7321 gaagttggac tcggccagga agccgccggc ctcggtcgtc accatgaagt actcgggggc
     7381 gtagccggtt cccacgtacg gaagcgcctg tcctcccgac atacggatct cgcctgaggg
     7441 cgggagctcg tagtgatcgg cgaagtgcgc ttccaggacc gcggccccgg gtgaggcggc
     7501 gtcggcgggt cgcaacgctc ggccgccggt gacgaagatc ttgttcagat gcggtccgcg
     7561 cgccccgaga tcgagaccga tcacgacgcc gggcaccagg atcgtacggc cgccgaacga
     7621 tgcgttcacc tgggtcggga ggacgacccg ttcctccgcc gcggtgacga cgccctgcga
     7681 gcggagatgg gcgattgcct cgacgagcgt ccccgagggc acccagctcc ccgccgggag
     7741 cgacacatgc acgtcgtgcg cgttcaacag cgagtagctt tcgtcgtagg agatgcgccg
     7801 ccaggtcgcg aggctcccga gcgccgcgaa catgcccgaa ccgatcgcga cgatcagcgc
     7861 gatggccgcg acctggagcc agcgggcgcg caggtcacgc ctcgaccacc ggagccagaa
     7921 cgcgacccta cgtatcatcg gcacctcacc aatgcaggtc ttcgatcctc gtcgtcccgc
     7981 ctcgcggagg accgtcgccg accttgccgc cgctcgacag ctcgatgacc cggtcggcga
     8041 tccgtgagat ctcgcggttg tgcgtgacga cgactaccgt cttgcccgcc tcggcctggc
     8101 tccgcagcag gtcgaggatc tggacacccg tccggaaatc gagctcaccg gtcggctcgt
     8161 cggccaacat gaccgggttg cctgtcgcga gcgcccgtgc gatcgcgacc cgctgttgct
     8221 ccccgccgga cagctcatgc gggaaatggt cgatccgctc gacgaggccc acgtcctcca
     8281 gcaccgcggc cgcgtcgacg ccgcgagctc ctgaaacgtc gacgccgaac tggacgttct
     8341 cgagcgcggt cagcccgggg aacagattga agctctggaa gatgaaacta accgtcgtcc
     8401 gccggaactc cgtgagcgct cgccgcccta cctcgccgat cgagcgtccg gcgatgatca
     8461 cgcgacccga cgtcggcgag tcgagcgagc cgatgacgtt cagaagcgtt gtcttgccgc
     8521 tccccgacgg cccgagaacg acaacgaact caccggcgtc cacttcgagg tcgacacggt
     8581 ccagggccgt cacgcagaca gctcccaact cgtagcgctt ggtgatctgc tcgaggagga
     8641 tcgccgtcgc cgcgccttcg cggtgtccag gatgcgagag gtaaccggaa tcggtgatct
     8701 tggtccaggc catgccgcct cctcactgag acgtcgcttc gaaggtaccg aggggtcgac
     8761 aagacctttc ggggcgaagg gcccgcatcg ccagggacca tcgggaaccg cagatcagtc
     8821 accgacgaca atcgaggacc ggacattcgc ccgccagccg atcggagggg tagccggtcc
     8881 acggcgatgt cgacatgttc gctctgctcc gccgtgaacg gtaccgtggt cgcgtggagg
     8941 acaccgatgg acaaggaggg ttcctcggca cggcccaacc tcacgaggcg tccggccgcc
     9001 gaggcgaagt cgtggagtcg cggccgcagc tgctcgatcc ctccttcccc atccctccac
     9061 ggccacagca acgccatctg aggcctcgcg ccaagtggat tgcctggggc attcagagtc
     9121 gtacgcatcg ctgactgctg tcctttcgat atgcgtcgtg ccgaccttcg cgtgacgccc
     9181 gctcaggcgg agcactgccc aacgcgatca tcaacccgcg ccgcttcgcc tggcgcatcg
     9241 cagtacgtag gctgcgccga ttgccagcag gcgaccttcc cggcgaccaa cgtcgtggcc
     9301 ccgtgacgaa cggcgcagtt gtggtggttg ccgctggaga ccgtgagccg gtgcgcgggc
     9361 cgcgagggtg gggtcgcgtc ttgcgtggtc ggggacgatg gaagatcgcc tccgcacgcg
     9421 ggcgctcaag aatcattcgg ccggttggcc ggcgttgggc tgagccttcc ttcagcacca
     9481 ctcagccgca tatgtgtgtc ttcgcccacc tgagccctcc tacagcgccg gcactgcctc
     9541 gatctgaggc ttgagctgcc aatctccaca agcaggacga tgtcctcgag cagttcccgg
     9601 gtcacgtccg cggagacctc gtgggctcgc ctgccgggga ggtccagcga agcgcgcgcc
     9661 cacgatagaa gggactgccc agggttcggt tgactccttg gactgccccg tgaagcgttg
     9721 agtcaggatg ctgggatcca tcacgcgacc tgtatcgggt aggggttcat catctcgaac
     9781 tcgacggggg tgagcatccc cagcgcgctg tgccg
//
LOCUS       DASUYD010000231         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1275453, whole genome shotgun sequence.
ACCESSION   DASUYD010000231 DASUYD010000000
VERSION     DASUYD010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1275453"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..619
                     /locus_tag="VF195_12200"
     CDS             356..619
                     /locus_tag="VF195_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581617.1"
                     /translation="MQMIPIPPIRELRIAFFPGGFEVEPEGLELLQAELGSVRFKWSG
                     TIDDEEEAFARAVVAASGPLITDAWDLDRSRQDREKVEELISP"
     gene            complement(616..1131)
                     /locus_tag="VF195_12205"
     CDS             complement(616..1131)
                     /locus_tag="VF195_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581618.1"
                     /translation="MGGAVPPERRWHSGIIDEHLAQLVAGQRSDITDVLKRRDDAPVE
                     RTERFGAHEALGLGQDHELPRGRRPGREPELAKMIPRVEEDGEGHGEGAPMKVTLDTN
                     VLQELWNDRPKRAVVERLLALAEEKGIDLAVTARVREDIPDEPLASKIRTLSDIGVEE
                     SGSDTRLGHWD"
     gene            1316..1546
                     /locus_tag="VF195_12210"
     CDS             1316..1546
                     /locus_tag="VF195_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581619.1"
                     /translation="MCYAHLKNRAMRGGALTEDEMEFVGTTVHWRDTGGGWRQPDTAA
                     YEIGSILSAEDAALLQSLMNAGSTDRVEFLGG"
     gene            complement(1631..2110)
                     /locus_tag="VF195_12215"
     CDS             complement(1631..2110)
                     /locus_tag="VF195_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX6581620.1"
                     /translation="MRRVIWIGLSVLFGGFLLIQLIPYGRDHLNPPVTAEPAWDSPET
                     RALAVAACFDCHSNETDWPWYTNIAPVSWLSQHDVDEGRDVLNFSDRPQEAEDVSEVI
                     QEGEMPPWYYGITHSGARLSDAEVERLISGLEATFLADPPLEGGGGESGEGGEAGEG"
     gene            2502..2945
                     /locus_tag="VF195_12220"
     CDS             2502..2945
                     /locus_tag="VF195_12220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581621.1"
                     /translation="MGSPATQPLVLVMDEFSFNVPGPLPPPGYPRPIAALPAGSSVLI
                     TLRNVGQLVHHWSVGRTLLAGGGYEDDLLARMEPEVLSDTGYRLVETEEGAAGGRGGV
                     IIEVSPGFDVTVRLAVPANATGTWEMACFIPGHYQNGMKAPLGVA"
BASE COUNT          548 a         1118 c         1092 g          576 t
ORIGIN      
        1 gctccggccc tcgagcacga ccgcgtccac cacgtaccga gccagatcca tcgcagaccc
       61 ccgtcatcgg tgtccacgat gctccgacac aggtgtccac tatccggtga actcgcgcac
      121 cccctccgac actcaactac cagcggaaac gcaagggccc ggagagcacg ttcgccctcc
      181 gggctctgtc acgctcccac cgcgctccca cgcgggtcgg atcacgcccc gatctcgtcg
      241 aggaagtcct ctggagcctg cactcgtatc ccgaaacggt ctcggagcac ctggctcaat
      301 cgaaggatcg ggcgatccca ggtgaggaag atggtgcggc ttggagcatg tgtgcatgca
      361 gatgatccca atcccgccaa tcagggagct tcggatcgcg ttctttccgg gcggattcga
      421 ggtggagccg gaaggtctcg aactcctcca agccgagctc ggctcggtcc ggttcaagtg
      481 gtccggcacg atcgacgacg aggaagaggc gttcgcgcgc gcggtcgtgg ccgcgagcgg
      541 gccgctcatc accgacgctt gggatctcga ccggtcgcgt caggatcgcg agaaggtcga
      601 ggagctcatc tctccctagt cccaatggcc gagtcttgtg tctgaacccg actcctcgac
      661 gccaatgtct gaaagcgtcc tgatcttgct tgcgagaggt tcatccggaa tgtcctctcg
      721 gactcgcgcc gtgaccgcga ggtcgatgcc cttctcttcg gcgagcgcca gcaatcgctc
      781 cacgacggct ctcttaggcc gatcgttcca aagctcctgg agaacgttgg tgtcaagcgt
      841 gaccttcatt ggggcccctt cgccgtgtcc ttcgccgtct tcctcgactc gcgggatcat
      901 cttcgcgagc tcaggttccc ggcctggtcg ccggcctcgt gggagttcgt ggtcctgacc
      961 aagaccgagc gcctcgtgcg ctccgaacct ttccgttcgc tccaccggcg catcgtcgcg
     1021 gcgcttgagc acgtcggtga tgtcggaacg ctggccggcg acgagctgcg cgaggtgctc
     1081 gtcgatgatc cccgagtgcc acctcctttc cgggggcacc gcgcctccca cgatgaccgc
     1141 cctggtgctc gggtcgagga tgacggcggt cgtcgaacgc ggatggggag cccgggcacc
     1201 aagccggctc gggctctccg accattgcta acgccgttag caggtgcccc ggtgaggccg
     1261 tagggcgttg gctcacgact gccctcacga tcgaacgccg agcttcggct gctcgatgtg
     1321 ttacgcgcac ttgaagaacc gggccatgcg cggcggcgcg ctcaccgagg acgagatgga
     1381 gttcgtcggc acgaccgtgc actggcgcga cacaggcggc gggtggaggc agccggacac
     1441 cgccgcttac gagatcggct cgatcctgtc agccgaggac gccgcgctgt tgcagtcgct
     1501 catgaacgcc ggcagcacgg accgcgtgga gttcctcggc ggctagaccc caaaccagcc
     1561 gcctggacag gagggaggtc ggggtctagc cggacgagat gcttagccca gcgagcaccg
     1621 gtgatcagcc ctacccctcg ccggcttctc cgccttctcc ggactcgccg cctccgcctt
     1681 cgagcggcgg gtcggcgagg aaggtcgcct caagaccgga gatcagccgc tcgacctcgg
     1741 cgtcggacag gcgagcgccg gagtgcgtga tgccgtagta ccagggtggc atctcgccct
     1801 cctggatcac ttcggacacg tcctcggctt cctgcggccg gtcggagaag ttgaggacat
     1861 ctcgcccctc gtccacatcg tgctggctga gccaggagac cggcgcgatg ttcgtgtacc
     1921 agggccagtc cgtctcgtta ctgtggcagt cgaagcaggc ggcaacggcg agcgcccggg
     1981 tctccgggga gtcccaggcg ggctccgcgg tcaccggcgg gttcaagtgg tcgcgcccgt
     2041 acgggatcag ctggatcagc aggaagcccc cgaagaggac ggacaggccg atccagatca
     2101 cacgtctcac gccgcaatcc tgacacgaaa cttgcccgac gggccggccg tcccggcgac
     2161 ggtaggacct tcggccgacg ccgttgacct tcggactggg cgggctcacc atgtcgcggt
     2221 cgactgtgtg cccttcaaca tcactgttca gcaattcatg gcggctggga cgccactctc
     2281 ggacgagtac aaacacggcg ccgtcctgag tcgccgaagt ttattggcct cgggaacgct
     2341 acgatggcgc catggttgtc cggggacggg gcaccggtaa ctggtggacc agacctgcct
     2401 ctctgatcgg ggtggccgcg ctgtcggctg tgctgggcgc aagctgcgac cgacccgctg
     2461 gccccgaacc gacggcgagc ccgaccaagg aggccgagca ggtgggatcg ccggcgacac
     2521 agccgctggt gcttgtgatg gacgagttca gcttcaacgt gcctggcccc cttccgcccc
     2581 ccggttaccc ccgaccgatc gctgccctgc cagccggtag ttcggtcctc atcacgctgc
     2641 ggaacgttgg gcagctcgtc caccactgga gcgtcggccg aaccctcctc gccggtgggg
     2701 gctacgagga tgacctgttg gcgaggatgg agccggaggt cctgagcgac acaggatacc
     2761 ggctcgttga gaccgaagaa ggggcggcag gagggcgagg cggcgtcatc atcgaggttt
     2821 caccaggatt cgacgtaacc gttcgcctgg cggtcccggc caacgcgacc ggtacgtggg
     2881 agatggcctg cttcatccct gggcactacc agaacgggat gaaggcaccg ctcggcgtcg
     2941 cgtaatacgg tcgactgatc ggcgccccaa cgaggaatgg tcaggttgtc gaacggaatc
     3001 tcgtggcgac ctccgcgctc ccaccgcgct cccacgccgg atggaagccg gctccaccgg
     3061 actccacgac gaccactagg ttcacgtatt cgcgcaggtc agcgcgctgg acggcaccag
     3121 aggacactcc ccgccacgtg cgttcctagg ttgagggcct agtggcctta tgggccgtgg
     3181 gggttcaaat cccccctccg acacgctgtg atgtgtcggc gcatcgtggc cggatgtgtc
     3241 ggcgcatcgt agccgctcct cacgatccct ggggttggta gtcgcgcgtc gggtcgagct
     3301 gcaaatgcct gagcagctca ccatcgaggg agat
//
LOCUS       DASUYD010000232        23302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_396796, whole genome shotgun sequence.
ACCESSION   DASUYD010000232 DASUYD010000000
VERSION     DASUYD010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396796"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..351
                     /locus_tag="VF195_12225"
     CDS             79..351
                     /locus_tag="VF195_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581622.1"
                     /translation="MAETAKRSRARGSTGPEPETHASGDQVCTVGFCPICLAVTAVQP
                     LKPEAIEHLLNAGREFLLAMTAVLGARANEAGGADAAPTLTRIDIE"
     gene            348..1298
                     /locus_tag="VF195_12230"
     CDS             348..1298
                     /locus_tag="VF195_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX6581623.1"
                     /translation="MSDRSAVGVDVGGTKIAALRMTVEGSIQASTVIATPASDQDAAL
                     PAIEAAIASVIDDDVVAIGIGMAGLVDARTGVLLTTPNLVWRNLPLEAHLHAAFSLPV
                     SVNNDATAAAWAERSLGASRDYDDSLFVGVGTGIGGGVVMGGQIVRGAHGLAGEIGHV
                     IVEPDGPVCGCGNRGCWEQVASGLAIARAGARTVADSPESAIGRLVGGDPRRVTGEIV
                     SQAAREGDGAATALLAEVGRRLGEGVAGLVNVLDPEIVVIGGGASEVGELLLAPLRAA
                     FVDSLEGADVRPEVPIVAAALGNDAGAIGAGLLALGKVAG"
     gene            1295..2161
                     /locus_tag="VF195_12235"
     CDS             1295..2161
                     /locus_tag="VF195_12235"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX6581624.1"
                     /translation="MRLGLSLPMFTDDAERPLAAAARAARVGYQGVFAPDHLFPPGAP
                     GRPALEPFAILAAVAARHPGLHIGTLVSRASLRSAATLAKQAAALDEMSGGRAILGLG
                     AGDSVSRAEHETFGIPFAPAARRVALLEETVGAMRALFAGTSWEGGAHVPAIAGPLLP
                     PGHPELWVGGRSDEVIATAARSADAWNGWAMDADRFAEAVHELRRVADGRTVSPTWGG
                     IALVGRDAADLERLRAERSARGLSMDVWQGEGPAFRAFADRLREMGCSWIVVLPVGGA
                     DRIEVVAEALER"
     gene            2158..2754
                     /locus_tag="VF195_12240"
     CDS             2158..2754
                     /locus_tag="VF195_12240"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEX6581625.1"
                     /translation="MNSGALKRAKRQIRRAVLEERDALSPGVRAEWGERIARRAVELP
                     EVRDVRAVMLFSSFGSEVPTAPLIERLRERGIVVALPRIEAGELVPVAYTPGDPVRAA
                     SFGALEPIRANGLEPASLDVVGVPGVAFDRLGGRVGYGGGYYDRFLRGLPAFRVGIAF
                     GMQVIEGPIPAGRFDLPVQAIVTEEETIRPDLNSRSIS"
     gene            2767..3867
                     /locus_tag="VF195_12245"
     CDS             2767..3867
                     /locus_tag="VF195_12245"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HEX6581626.1"
                     /translation="MDETSRKTAFHEIMAKTAAGFMEEAGWFWVTGFGDPEAEYEAIR
                     NGVGVWDVSPLNKWDFRGPDAVEAAQRVHSNDIVGMQTGQVRYGAFLDEDGLLVDDGT
                     VFKHADDHLWVCTNGLDHEAYFADATKGLDVAVDYIAPDLPHLQVQGPRSRDALQPIT
                     DADLGSIGYFRFLPQPVQVGGVPVWLSRTGFSGELGYELFTKPEHAEDLWTAIEGIGA
                     VPYGVDVVEPVRIEAGMIVTDYDYEAHQRTPYDLGLDRLVALDGEGSFMGKEALREVA
                     AAPPNRFKTLRLEGQELPEYGATVTKDGAEAGVLTSPASSPTFGPIGLAILRSDVAAD
                     GTAVDVALGDGTIGATVDVLAVVDPGKQRPRG"
     gene            3896..4792
                     /gene="ftcD"
                     /locus_tag="VF195_12250"
     CDS             3896..4792
                     /gene="ftcD"
                     /locus_tag="VF195_12250"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HEX6581627.1"
                     /translation="MDAIVGVVPNFSEGRRRDVIDAIVDALEVDGARVVFAEADPDHH
                     RLDTTVLGSAGGVARAAVSGAAVAIDRIDLFEHRGGHPRMGAVDVVPFMPVRGVEMEE
                     CVELARSVGRELAEAFSLPVYLYGEAAVVPERRSLADVRKGEFEGLRAAVAAGDRLPD
                     FGPHELGRAGATAVGARKPLVAFNTYLRGGDEEAAKEIAREVRESAGGLRAVRAIGFA
                     VPERGCVTVSMNLVDFEVTGVGAAFDAVVRAAAARGLEVLDSEIVGLAPEAALPEGTM
                     EHVRLAGFSPETQILEGLVETG"
     gene            4824..5456
                     /locus_tag="VF195_12255"
     CDS             4824..5456
                     /locus_tag="VF195_12255"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HEX6581628.1"
                     /translation="MGTRDDAIGSWLTELGSASPTPGGGAAAGVGAAMGAALIAMVGH
                     LTVGKEGFEDLDARMRSLVETAEAEREAFLALADEDAAAFGSVMAAFRLPNGTDEQKA
                     SRTMRIQEAYAGAAAVPLQLARRCVRLMDLAEDATAMGNPHASSDGYSAGLLLFAAAL
                     AATANVRINAAGLKDEATGQGLVDECDALRARADALLQQIEEAFLLRLSA"
     gene            5507..5929
                     /locus_tag="VF195_12260"
     CDS             5507..5929
                     /locus_tag="VF195_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016337385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581629.1"
                     /translation="MRIESVVFDCHDAAPLARFWADALGWSVAPYDEEELERLASKGI
                     YDPEDDPSVMVQPPADTDLPVLFFTEVPEEKAAKNRVHLDLGADGPLEGEVLRLEELG
                     AHVRNWAEDDGSTWCVMLDPEGNEFCVVTASDEEDEDD"
     gene            complement(5933..6793)
                     /locus_tag="VF195_12265"
     CDS             complement(5933..6793)
                     /locus_tag="VF195_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581630.1"
                     /translation="MAASRGTRVFATWPRPERRATILLVSATATASAPPRTLGDMMAA
                     RLRAAAIVAIGSGIAGALVGGLGSRLVMRLAALAAPEVRGAVTENGAIVGEITLTGTV
                     ALVVFAGVASTALGAGAFVLVKPWLPERTVLRGMVFGGFLLATMGASVIDAGNADFVI
                     LGDRLLNVAIFSSLFLAFGVVASAASTALEARVPEAAALSPRLWAVSAICALPLIPGV
                     IGMAFGFGPRLGVPLLAASAAMCAVPSVERRGRHGLAQLIRVGATVGMLVVVGLAGAD
                     YVDNVITILR"
     gene            6831..7028
                     /locus_tag="VF195_12270"
     CDS             6831..7028
                     /locus_tag="VF195_12270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581631.1"
                     /translation="MPDERVSAILKTADVSTPPGDPRFMSWGSRELGLRDPNGYFLTF
                     TEDAGSFLGGQENEPERDGSA"
     gene            complement(7025..8695)
                     /locus_tag="VF195_12275"
     CDS             complement(7025..8695)
                     /locus_tag="VF195_12275"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006569599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX6581632.1"
                     /translation="MKSGLQIAQEAVLRPITDVAADAGILESELEQYGRSKAKVSLSI
                     LERLADRPDGKLVITTAITPTKAGEGKTTTSISLAQGMGKIGKDVMLCLREPSMGPVF
                     GIKGGGNGGGYAQVVPMEEINLHFNGDFHAVQAAHNLLAAALDASIYHGNALGIDPVS
                     VTWPRTLDVNDRELRYSVVGLGGRAHGVPRENQFVIVAASEIMAVLALSNDLADLRAR
                     LGRIVVASTADGTPVTAEDLRVAGAMAVIMKEAIKPNLVQTLEGQPALIHAGPFGNIA
                     HANNSIIADRIALKLADYVVTEAGFASDLGFQKFCDIVCRFGRFAPSAGVLVTTVRAT
                     KSHGGMPFDQLGTEDLDTLRRGIDNLEAHIGIVREYGLPCVVSINAFPTDTEKEIETI
                     RELAGGAGAETVVVNRAFAEGGAGATELAEAVVSACERPSTFHQLTPEGAPLREQIDA
                     IATKIYGADGVEYQAQAEKDIARMEQLGFGAMPVCMAKTHLSLSHDPLLLNRPKGYRL
                     PVRGVVPSAGAGFVVVLCGDMQRMPGLGRTPAFTQVDLEPDGRTVGLF"
     gene            8859..10256
                     /locus_tag="VF195_12280"
     CDS             8859..10256
                     /locus_tag="VF195_12280"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEX6581633.1"
                     /translation="MATDIEQFVERPGRDDAIKEVRRQIEAEGVTYMYYQFVSVTGRI
                     MGKGIPAKHWETIAHKGFQLVYGSTANLFVDRLGNYIGYGPEARELVGIPDVETFCVL
                     PWDRKTARVFCTLFRGREEEVDGGAFLTSDCRGNLKRLHDEFTEETGLHLRAGTEPEM
                     MWLKTNPDGTPSVDGKTKPYCYHIDQFSELQPIIHKVMEYGEAMGLDMIQGDHEDAPG
                     QLELNFMFDKAEATADRLTTYRQICRQVGREMNAFACFMPKPFMGVSANGCHHNISLW
                     QGDENMFMPDGDDPRVPGKIGLHAIGGVLEHLRALTCITSPTVNSYRRFADAGFWAPI
                     FADWGFQNRTTALRISAPGRYEYRSVDSAVNPYLSFAALLMAMKDGLARNLDPGPPEE
                     RNIYEAIAEGKDVQRIPETLGEALEALEADDVVKAALPGDMYRVFMHYKTDEWKRFIG
                     TVSDWDVAEYLDVLP"
     gene            10306..11235
                     /locus_tag="VF195_12285"
     CDS             10306..11235
                     /locus_tag="VF195_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006997981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX6581634.1"
                     /translation="MCGIAGIIHRDGSSDVGSEMTRMLQAMKHRGPDSSGYALFGEPS
                     ELVVIRFKLADANDPRDFDFKERLERHRQEVESRLHKIGASVDRVERETEYAYRATAR
                     YDGDMKALADYLEDVPGCEVLSLGHSLEIVKDLGDAQEVSDLYRLGGFNGTHAIGHVR
                     MATESDVDISGAHPYWAYPFSDVAVVHNGQLTNYFHWKRRLESSGHRFQSECDSEIIA
                     VYLAEKMADGDSLEDAMHKSLDELDGVFTYMAVTQDALGVAKDEMAAKPLVLYESDGI
                     VAMASEEIAIRAVLDREIDTTDPYEGQVLVWTR"
     gene            11232..12014
                     /locus_tag="VF195_12290"
     CDS             11232..12014
                     /locus_tag="VF195_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018402081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GXGXG motif-containing protein"
                     /protein_id="HEX6581635.1"
                     /translation="MSGARRTTYDARGLAEPHAEVPKVSDIDGTTASFDATDLTTRQI
                     NLELRWLLYEQGMRDVTVRNPGAKHSIGVGILQRCRIRYEGSLGYFGLGLIDGPEITV
                     TGRVGWSVCENMMSGVVVIEKNAGSLTGAAMRGGDLLVHGSVGARTGIDQKGGTIIVG
                     GDAGTMTAFMMQRGRQIILGDVGPGLGDSMYDGEIYVAGSVKSLGIDCVEGEWTDADT
                     ELIERKFRIYGLGSPPAFQKFVCGKMLYNYDNLEPSEKKLVI"
     gene            12036..13439
                     /locus_tag="VF195_12295"
     CDS             12036..13439
                     /locus_tag="VF195_12295"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020044962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HEX6581636.1"
                     /translation="MAEVSSRKEVLGRSPIFTEEVINDIHVKAELGRYRMRGFSMFKP
                     MPGWDQLMFLPGTLTRFVIEGYREKCETKTVLGARFAKHPIELDIPIYITGMSFGALS
                     LEAKMALAKGASMAGTATCSGEGGMIPPERDLSTKWYYQNIQSRYGFNPHHLMLADAC
                     EFFIGQGCKVGLGGHLMGQKVTEQVAEMRSLPAGIDQRSPARHPDWLGPDDLSLKVQE
                     VREATDYRIPIQLKLGAARVYDDVRMAAKCGPDIIYLDGAEGSTGAGPHIAAEETGIP
                     LMAAIPEARRALEDVGLADEVDLVVAGGIRNGADVAKCLALGATAVAIGHSALMALNC
                     NKEIPGVTDYEGTVGVPAGQCYHCHTGRCPVGITTQDPELRKRLEVDSAAERVYNFLT
                     AMTMELQMLARACGKTDVHSLEPEDLCALTVEAAAMAKVPLAGTDYIPGVTDKETMED
                     IKAMMQKYLADTGQFPG"
     gene            13463..13900
                     /locus_tag="VF195_12300"
     CDS             13463..13900
                     /locus_tag="VF195_12300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581637.1"
                     /translation="MTEPHHLSTKIGIDAEELAGRRFPYQEDISLVEDLDLDELTPGE
                     DLNWLEDVTLLQEDGVAAVFDRYSNSFLKIHFEIPAGREDELARKVLMKHLIEGNSYG
                     IQLKKQHAKFAQPELGPWVEGSETVGTDWKPAVMEGWERPALH"
     gene            13919..14710
                     /locus_tag="VF195_12305"
     CDS             13919..14710
                     /locus_tag="VF195_12305"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEX6581638.1"
                     /translation="MTVRRPLIAISEGFADYGDYYGYGYGRPLLAAGALPVLLPYYER
                     EEDRFDLIERIDGLVLAGGRDIEAWRYGREEPHPQHLPGQPFLDEIDLSYALLAVEDD
                     VPVLGVCRGCQVLNVALGGTLWGDLGEFPGGGADHPGAKWDEWRALVAATLEGGARPA
                     HPTHPIEVRPGSLLAAHLGERYEVDSYHHQAVRELGTGLEAVAWAPHGTIEAVEMPGS
                     PAFVLGVQWELHEEWQDDERMLGIWEMFVEDARRRMEERAEVPVT"
     gene            14707..15069
                     /locus_tag="VF195_12310"
     CDS             14707..15069
                     /locus_tag="VF195_12310"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581639.1"
                     /translation="MTASIEVTIDAVDGPAAVAFWQQALGYERRSARGGYVVLGPPAE
                     DPRPAVVIQHVDAVSPGKTPVHLDLRVDDVDAEVARLQALGATIGWTIDETAEGGSRW
                     TTMSDPQGTLFCVCTARS"
     gene            15106..16440
                     /locus_tag="VF195_12315"
     CDS             15106..16440
                     /locus_tag="VF195_12315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018401404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX6581640.1"
                     /translation="MTLPEHARYVVIGAGIHGLSTAWHLAKELESRGQGDGRDVVILD
                     KTGVGAGPSGIACGVIRNNYFQPAMRELMAHSVAIWESDPEAFHYHPIGYLQIAPEVM
                     RADVAMIHEQQKAIGYTSELIEGEDDCRRYMESIFSDWQARNITAVLHEKKGGYAENL
                     PSMEGLRSKSEALGVALHAPVWVTGFRRDGGVVRAVETDRGAIECEQVVIAAGPWVRD
                     FWHMLDLPGEIDVLGRDGKVYERPMWTYWCLQEGTLGVDPTYLLDNEGNMPPVVHIDT
                     DAPLVDDMDGSIVTEDLWGIYYKPDVNFGGVQGGAMPWVVEQPADEVAVDPYGHLSTE
                     FVVTAEFERMWTSALAHCHKRFEGKRPLYKKAPTGGIGAFTPDSFPVFDRFVDNAYVV
                     ADSNHGYKMIGIGQLVAKELLGEQQALLEPFRFSRFAEGRLHPVSQSPYPWS"
     gene            16442..17422
                     /locus_tag="VF195_12320"
     CDS             16442..17422
                     /locus_tag="VF195_12320"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HEX6581641.1"
                     /translation="MTDQPTTRALPVEFDLPVHGGFAPAGIGETFPDDAPDAEELEIA
                     GEPGQDPVAAFRRTLGMFATGVTVITTQSGDQVHGMTANAFMSVSLRPPLVVISVDRR
                     ARMHSLLNERSRYGISVLADEQRSLSDRFAGRGVGDDQEVEFDVVHETPLVRGALAHL
                     VARVVRSSWGGDHSLFIGQVEYARYGEGQPLLFHGGRYEHLLERAPVFSSLPPEILAA
                     ITAEGDERMFEPGEFVVREGETGDELFVILEGEARVERASNVLTMFRDGDFFGEISVF
                     DGRPRSADVVAATPLRTLAISRELVRAALEREPLAAWAMLEVLAGRLRDD"
     gene            complement(17444..19828)
                     /locus_tag="VF195_12325"
     CDS             complement(17444..19828)
                     /locus_tag="VF195_12325"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX6581642.1"
                     /translation="MKQAQSETRTPEETIERPARPERSDRASRFKRRIVGAPMATERL
                     IHERLGKPTALAVFASDNLSSSAYATEEIVRVLMFAGLGTVAFAKVVPITIALLIVLA
                     ILLFSYRQTIRAYPQAGGAYLVTRDNFGVKPAQVAGVALLTDYVLTVSVSVAAGTAAL
                     TSIYEGLHPWRVWIAIGFVVILTWGNLRGVRESGRLFAAPTYFFLLMMGVLLGVSFFR
                     LFAGTLPTSPSTFPVPETVSISGAALVFLVLRAFASGGAAVTGVEAISNGVPAFKPPE
                     WKNAITTLMWMGTLLGAMFLGLSILAARMQIVPDEHEKVTVNAQIARAVFGDSGVGDV
                     LFVLVQVATMLILVLAANTSYADFPRLASFHAGDHFLPSQLTRYGDRLVFSNGIIVLS
                     IFASLLLVMFDASVTNLIPLYAIGVFTSFTFSQAGMAKRHTTLREQGWRKGLAINGFG
                     AVVTAVMTVIIAATKFADGAWIILIAIPVMLLGLLRVNHHYEETAAALVDPGRRGPVR
                     DLPRQRVVIPVRTPGPEDVYATAYAKRVFPLEVRLVHFAPTGTDIAFMWDSWGRLGEM
                     LELRLRTRSIPAEIRNLAREIRAGADEDALINVIIPESVRYRGWRHFLHKLLVQRIKA
                     ALVAEADVVVTNVAHHPGYDSLEPVTADGDVRRAMEGWRHVAVVLASGAHNATDRSLR
                     YAMSLRADELRCLHVDVDGKASEELRARWQERYPDVPIEVLDSPYRQVGGPIHAWVRE
                     VLDEHPKTFVTLVIPEFVVRKWWHRLLHNHTAMTLKGTFLFEPSVVVSAVPYRL"
     gene            19954..21420
                     /locus_tag="VF195_12330"
     CDS             19954..21420
                     /locus_tag="VF195_12330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581643.1"
                     /translation="MRKVLAPRPASITAILAIGILASASAVGIALAIGSKNLAAATAL
                     CLLGVVAAGAVAGRGSAIVASFLSFLGLNFFFTEPHHTLAVRHASDVVALVAFLLSGL
                     IVGALVSRVREERSRAERRATEALFLNRTTERFISTESIDRTLAGLAEALLGFFGLVR
                     CDISTSVGVGRAGTDDPGPVVGQTISTPLVTPSGSFGALTVVRAASEDPLAPPELGLL
                     HTLAAQTALAIERAALDGEVRAARIEAEAHALRAALFSSVTHDLRTPLSSIKASASGL
                     LAEGAHYSDEERRDVLRTVVEEADHLNLIVGNLLDLARIRAGALVPSKQTILVEEVIG
                     SVLQRMRRSLEGLVVRTTIRPELPPVDADPVQIGQVLSNIIENAIRFSPNGSEIQITA
                     ARWHSAVQVRITDQGPGIPAEDRDRVFEEFYRRDAGAGRGGTGLGLAIARAVVVAHGG
                     TISARSAPGGGTAIVFELPIAVGETHDEASRAPRSAVS"
     gene            21417..22115
                     /locus_tag="VF195_12335"
     CDS             21417..22115
                     /locus_tag="VF195_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581644.1"
                     /translation="MTRVLVIDDEPQILRALRRSLEAQDYEVATAEDGEAGLALAAAR
                     TPNLVVLDLGLPDLDGTEVIRRLRSWTDVPVIVLSVREGRSDKIDALDAGADDYVTKP
                     FDVEELLARMRAALRRADADLAVEPEVRYGDLTIDLARRLVRLGDAIVHVTPNEYALL
                     AAMVTHPGKLLTHQWLLREVWGRGYANESHYLHVYVRQLRQKLGDDAAMPRFIITEPG
                     VGYRWNPDAAVERR"
     gene            complement(22112..23002)
                     /locus_tag="VF195_12340"
     CDS             complement(22112..23002)
                     /locus_tag="VF195_12340"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX6581645.1"
                     /translation="MAPPLESFAPDQVEAFHRDGFLIVEEGLISQKALDLLEERYLRL
                     FDGEYETGIKPDEVNWVKGRDPEDRTRQLCNAWRADSVIAAQVLSARIGRLAAQLMSY
                     QGVRILQDNVLWKPPGTKAIGFHQDGSYADYLVPPEMVTCWMSLHETQADAGPIAYVR
                     GSNHWPWTPPERSQFHAPEDWLAGVRDAKPADAEMEIVPVVVRPGGGAFHHNLAWHGS
                     EPNTSGTVARMALVSHMLPSHARFHHRNVDLIYSRYRRRGDTSMDESFFPILWDETGH
                     RTSWLSDLPAIEEPIEEAVD"
     gene            complement(23005..>23302)
                     /locus_tag="VF195_12345"
     CDS             complement(23005..>23302)
                     /locus_tag="VF195_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008067927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6581646.1"
                     /translation="AERRAHPQRALVGLELDGNEPAAHGDGVYAGRQQVGVVTSGTRS
                     PILKKNVALARVAIQHRDLGTDLEVGKLDGQQKRIAAKVVRFPFYDPDKTRPRS"
BASE COUNT         3751 a         7688 c         8314 g         3549 t
ORIGIN      
        1 ccgcgaggcg aacttcgcgg gcgatcatct cgagatcctg ttcggacggg agccccgtcc
       61 ggtcgaaagg agctgaccat ggccgagacc gcgaagcgga gccgggcacg agggtcgacg
      121 ggccccgagc cggagacgca cgcttccggc gatcaggtct gcacggtggg attctgcccg
      181 atctgcctgg ccgtcaccgc cgtccaaccg ctgaagcccg aagcgatcga gcatctcctg
      241 aacgccggcc gcgagttcct gctcgcgatg acggcggtac tcggggcgcg cgccaacgag
      301 gccggcggtg ccgacgccgc tccgacgctc acgcgtatcg acatcgagtg agcgaccgca
      361 gcgcggtcgg cgtcgacgtc ggcggcacga agatcgccgc cctccggatg acggtggagg
      421 ggtcgatcca agcctccacc gtgatcgcca cgccggcatc cgaccaggat gccgcgctcc
      481 cggcgatcga ggcggcgatc gcgtcggtga tcgatgacga cgtggtcgcg atcgggatcg
      541 gcatggcggg cctcgtcgac gctcggacgg gcgtgctcct gacgaccccc aacctcgtct
      601 ggcggaacct gccgctcgaa gcgcacctgc acgccgcgtt ctccctgccc gtgagcgtga
      661 acaacgacgc gacggctgcc gcgtgggccg agcggtccct cggtgcctcc cgcgactacg
      721 acgactccct gttcgtcggc gtggggacgg gcatcggagg cggggtcgtc atgggcggcc
      781 agatcgtccg gggagcgcac ggtctcgcgg gcgagatcgg gcacgtcatc gtcgagccgg
      841 acggtcccgt ctgcgggtgt gggaaccgcg gatgctggga acaggtggcg agcggcctgg
      901 ccatcgcgcg ggcaggcgct cgcacggtgg ccgactcccc cgagagcgcg atcgggagac
      961 tcgtcggcgg cgacccccgt cgcgtgaccg gtgagatcgt gagccaggcg gcccgcgaag
     1021 gcgacggtgc cgccaccgcg ctcctcgctg aggtgggccg aagactcggc gagggagtgg
     1081 cgggcctcgt gaacgtcctc gatcccgaga tcgtcgtgat cggaggaggg gcgagcgagg
     1141 tgggagagct gctcctcgcg ccgctccgcg ccgcgttcgt cgactcgctc gaaggtgccg
     1201 acgtccgccc ggaggtgccg atcgtggcgg cggcgctcgg gaacgacgcc ggcgcgatcg
     1261 gcgccggcct gctcgcgctc gggaaggtcg ccggatgagg cttggactca gtctcccgat
     1321 gttcacggac gacgccgaac gaccgctcgc cgcggcggcg cgagcggccc gcgtcggcta
     1381 ccagggggtg ttcgcccccg atcacctctt cccaccgggg gcgcccggcc ggcccgcgct
     1441 cgagcccttc gcgatcctcg ccgcggtggc ggcccgccat cccggactgc acatcgggac
     1501 gctcgtctcg cgagcctccc tccggtccgc cgcgaccctc gcgaaacaag cggcggcgtt
     1561 ggatgagatg agcggtggcc gggcgatcct cggcctcggg gccggcgact cggtttcgag
     1621 ggcggagcac gagacgttcg gcatcccgtt cgcccccgcc gcacgacgag tggcgctcct
     1681 ggaggagacg gtcggagcga tgcgcgctct gttcgccgga acgtcgtggg agggcggcgc
     1741 ccacgtcccc gcgatcgcgg gcccgttgct tccgcctggg catccggagc tgtgggtcgg
     1801 ggggcgctcg gacgaggtca tcgcgacggc ggcccgatct gccgacgcct ggaacggctg
     1861 ggcgatggac gccgatcgat tcgcggaggc cgtgcacgaa ctccgccgcg tggcggacgg
     1921 ccggacggtg tctccgacgt ggggtgggat cgccctcgtc ggcagggatg ccgccgacct
     1981 cgaacgcctc cgagccgagc gcagtgccag gggcctgtcc atggatgtct ggcagggtga
     2041 aggcccggcc ttccgtgcgt tcgccgaccg tctgcgggag atgggctgct cgtggatcgt
     2101 cgtgcttccc gtcggcggcg ccgatcggat cgaggtcgtg gccgaagcac tggagcgatg
     2161 aacagcgggg cgctgaagcg cgcgaaacga cagatccggc gggcggtgct cgaggagcgc
     2221 gacgccctgt caccgggcgt gcgcgccgag tggggggagc ggatcgcccg gcgagccgtg
     2281 gagctgcccg aggtccgcga cgttcgagcg gtgatgttgt tctcgtcgtt cggatccgag
     2341 gtaccaacgg cgccgttgat cgaacggctc cgcgaacgtg ggatcgtcgt agcgctcccc
     2401 cgcatcgagg cgggggagct cgtcccggtc gcgtacacgc ccggggatcc cgtgcgggcg
     2461 gcgtcattcg gtgccctcga gccgatccgt gcgaacggtc tcgagccggc gtcgctcgac
     2521 gtggtggggg ttcccggcgt cgcgttcgat cgactcgggg gccgcgtcgg gtacgggggc
     2581 ggctattacg accggttcct ccgaggcctc cccgcgttcc gggtcggcat cgcattcggt
     2641 atgcaggtga tcgagggccc gatccccgca gggaggttcg atctccccgt ccaggcgatc
     2701 gtgacggagg aggagacgat ccgaccggac ttgaacagcc gttcaatctc gtgataagag
     2761 gggcgcatgg atgagacgag caggaagacg gcgttccacg agatcatggc gaagacggcg
     2821 gcagggttca tggaggaggc cggctggttc tgggtgacgg gcttcggcga tccggaggcc
     2881 gagtacgaag ccatccggaa cggggtgggt gtctgggacg tttccccgct caacaaatgg
     2941 gacttccgcg gtccggacgc ggtcgaggcc gcgcagcggg tccactcgaa cgacatcgtg
     3001 gggatgcaga ccggacaggt gcgctacggc gcgttcctgg acgaggacgg cctcctcgtc
     3061 gacgacggca cggtgttcaa gcacgcggac gaccatctct gggtctgcac gaatgggttg
     3121 gatcacgagg cgtacttcgc cgatgccacg aaggggctcg acgtcgccgt cgactacatc
     3181 gcgcccgacc tgccacacct ccaggtgcag ggtccccgct cgcgggacgc cctgcagccg
     3241 atcaccgacg ccgacctggg gagcatcggg tacttccggt tcctgccgca gcccgtgcag
     3301 gtcggcggcg ttccggtgtg gctgtcgcgc accggcttct cgggtgagct cggctacgag
     3361 ctgttcacga agcccgagca tgccgaggac ctctggacgg ccatcgaggg catcggcgcc
     3421 gtcccctacg gggtggacgt cgtcgaaccg gttcggatcg aggccgggat gatcgtgacc
     3481 gactacgact acgaggccca tcagcgcacg ccgtacgacc tcgggttgga ccggttggtc
     3541 gccctcgacg gcgagggttc gttcatgggg aaggaggcgt tgcgggaggt cgcggcggcc
     3601 ccaccgaacc ggttcaagac gctccggctc gaggggcagg agctccccga gtacggcgcg
     3661 accgtcacga aggacggtgc ggaggccggc gtgctgacct cgccggcgag cagcccaacc
     3721 ttcggcccga tcggcctcgc catcctccgg tccgacgtcg cggccgatgg gacggccgtc
     3781 gacgtcgccc tcggtgacgg gacgatcggt gcgacggtcg acgtgctcgc cgtcgtcgat
     3841 cccgggaagc aacgccccag gggctgatcc ccgccgctcc cgtaggctcg ccggcatgga
     3901 tgccatcgtc ggcgtggtgc cgaacttcag cgagggacgg cgacgcgacg tgatcgatgc
     3961 gatcgtcgac gcgctcgagg tggacggcgc gcgggtcgtg ttcgccgagg ccgatccgga
     4021 tcaccatcgc ctcgacacga cggtcctcgg gtcggccggc ggcgtcgcgc gggccgctgt
     4081 ctctggagcc gccgtggcga tcgatcggat cgacctgttc gagcaccgcg gcggccaccc
     4141 acggatgggt gcggtcgacg tcgtgccgtt catgcccgtg cgaggggtcg agatggagga
     4201 gtgcgtcgag ctcgctcggt cggtcggacg agagctggcc gaggcgttct ccctccccgt
     4261 gtacctctac ggggaagccg cggtcgttcc cgagcgtcgc tcactcgccg atgtgcggaa
     4321 gggcgagttc gaggggctcc gcgcggccgt cgcggcgggc gatcgactgc ccgacttcgg
     4381 tccgcacgag ctcggacgcg cgggtgcgac cgccgtcggg gcgcggaaac cgctggtcgc
     4441 cttcaacacc tacctgcgcg ggggcgacga ggaggctgcg aaggagatcg cccgcgaggt
     4501 ccgcgagtcc gcaggtggcc tccgcgccgt tcgcgcgatc ggcttcgcgg tgccggaacg
     4561 agggtgcgtc accgtttcca tgaacctcgt ggacttcgag gtgacggggg taggggccgc
     4621 gttcgatgcc gtggtgcgcg cggcggccgc ccgcgggctg gaggtgctgg actccgagat
     4681 cgtcggactc gcaccggagg cggccctacc tgagggaacg atggagcacg tgcgcctcgc
     4741 cggcttctcg ccggagacgc agatcctgga agggctcgtc gagaccggct gaggccgatc
     4801 tcgtgggaag gaaggagacc tcgatgggca cacgcgacga cgcgatcgga tcgtggctga
     4861 ccgagctcgg ctcggcatca ccgacgccgg ggggtggtgc cgccgccggg gtcggcgccg
     4921 cgatgggggc ggccttgatc gcgatggtgg ggcacctcac cgtcggcaag gaggggttcg
     4981 aggacctgga tgcgcgcatg cgctccctgg tggagacggc cgaggccgag cgggaggcct
     5041 tcctggcgct cgccgacgag gacgccgccg cgttcggatc ggtgatggcg gccttccgac
     5101 tccccaacgg gacggatgag cagaaggctt cgcggacgat gcggatccag gaggcgtacg
     5161 cgggcgccgc cgcggtcccg ctccagctcg ccaggcgctg cgtccggttg atggacctgg
     5221 cggaggacgc gaccgcgatg gggaatcccc acgcgtcctc cgacggctac tcggcgggcc
     5281 tcctcctgtt cgccgccgcc ctcgccgcca cggcgaacgt ccggatcaac gccgccggtc
     5341 tcaaggacga ggcgacgggt caggggctcg tcgacgagtg tgatgcgttg cgagcgcgcg
     5401 ccgatgccct cctccagcag atcgaggaag cgttcctcct gcgtctgtcc gcctgaaggc
     5461 tgcagcctga gcgcgcacct gctgcttggc ctaggcttcg aggacggtga ggatcgaatc
     5521 ggtcgtgttc gattgccacg atgccgcgcc gctcgcccgc ttctgggcgg atgcgctggg
     5581 ctggtccgtc gccccgtacg acgaggagga gctcgaacgc ctcgcgtcga aggggatcta
     5641 cgatcccgaa gacgacccat ccgtgatggt gcaacctccc gcggacaccg atctgcccgt
     5701 cctgttcttc accgaggttc cggaggagaa ggccgcgaag aatcgggtcc acctggacct
     5761 gggggcggac gggcccctcg agggcgaggt gctgcgcttg gaggagctgg gggcccacgt
     5821 ccggaactgg gctgaggacg acgggagcac ctggtgtgtc atgttggatc ccgagggcaa
     5881 cgaattctgc gtggtcaccg cgtccgacga ggaggacgag gacgactgac gcctatcgga
     5941 ggatcgtgat cacgttgtcg acgtagtcgg cgccggcgag cccgacgacg acgagcatcc
     6001 ccaccgtcgc gccgacgcgg atcagctggg ccaggccgtg acggccgcgg cgctcgacgg
     6061 acggaacggc gcacatcgcc gccgacgcag cgaggagagg aaccccgagc ctcggtccga
     6121 acccgaacgc cataccgatc acgcccggga tcagcgggag ggcgcagatc gcggagaccg
     6181 cccacagccg aggcgagagc gccgcggcct ccggcacccg cgcctccagg gccgtggatg
     6241 ccgcagaggc gaccacgccg aacgcgagga acagggagga gaagatcgcc acgttcagga
     6301 ggcgatcccc caggatcacg aagtcggcgt tgccggcgtc gatcaccgag gctcccatcg
     6361 tcgccagaag gaacccgccg aagaccatcc cgcggaggac ggtccgctcg gggagccacg
     6421 gcttcaccag gacgaaggcg ccggcaccga gcgccgtgga tgccacaccc gcgaagacca
     6481 ccagcgcgac ggttcccgtc agcgtgatct cgccgacgat cgcgccgttc tcggtcaccg
     6541 cgccacgaac ctccggagct gcgagtgccg ccagccgcat caccaggcgg ctcccgaggc
     6601 ccccgacgag cgctccggcg atgcccgagc cgatcgccac gatcgccgcc gcccgcaagc
     6661 gtgcagccat catgtcaccg agggttcgag gcggagcgga ggcggtcgcg gtagcgctca
     6721 cgagcaggat cgtcgcacgc cgctcggggc ggggccaggt ggcgaacacc cgggtcccgc
     6781 gggatgcagc cacccgcagg cgggctcgac gctcgacgta tcatcccgac gtgcccgacg
     6841 agcgagtgtc cgcgatcctc aagaccgcag acgtctcgac cccgcctggg gacccgaggt
     6901 tcatgagttg ggggtcgcgg gagctcgggt tgcgagatcc caacgggtac ttcctgacgt
     6961 tcaccgagga tgccggatcg ttcctcggcg gccaggagaa cgagccggaa cgggacgggt
     7021 ccgcttagaa caagccgacg gtccgaccgt cgggctcgag gtcgacctgc gtgaacgccg
     7081 gcgtccggcc aaggcccggc atccgttgca tgtctccaca gagcacgacg acgaagcccg
     7141 cgccggcact cggcacgacg ccgcggacgg gcagccggta tcccttcggt cgattcagga
     7201 gcagggggtc gtggctgagc gacaggtggg tcttggccat gcagacgggc atcgctccga
     7261 agccgagctg ctccatcctg gcgatgtcct tctcggcttg ggcctggtac tcgacgccgt
     7321 cggcgccgta gatcttcgtc gcgatcgcgt cgatctgctc cctgaggggc gcaccctcgg
     7381 gcgtgagctg gtggaacgtg ctcggtcgct cgcacgccga gacgacggcc tccgcgagct
     7441 cggtggctcc cgcgcctccc tccgcgaacg ccctgttcac caccaccgtc tcggcgccgg
     7501 cgccacccgc cagttcccgg atcgtctcga tctccttctc ggtgtcggtg gggaaggcgt
     7561 tgatcgagac cacgcacggg agcccgtact cgcggacgat gccgatgtgt gcctcgaggt
     7621 tgtcgatccc gcggcggagc gtgtcgaggt cctccgtgcc gagctggtcg aacggcatgc
     7681 cgccgtggga cttcgtggcg cgcacggtcg tgaccaggac accggcggac ggagcgaagc
     7741 ggccgaaccg acacacgatg tcgcagaact tctggaaccc cagatcggag gcgaagcccg
     7801 cctcggtcac gacgtagtcg gccagcttca gggcgatgcg atccgcgatg atcgagttgt
     7861 tggcgtgtgc gatgttcccg aagggtcccg cgtggataag cgcgggctgc ccctcgagcg
     7921 tctggacgag gttcggtttg atcgcctctt tcatgatgac cgccatcgca cctgcgacgc
     7981 ggaggtcctc cgcggtcacg ggcgtcccgt cggccgtcga ggcgacgacg atccgcccga
     8041 gtcgtgcgcg aaggtcggcg aggtcgttcg agagcgcgag cacggccatg atctccgagg
     8101 ccgcgacgat cacgaactgg ttctcacgcg ggacaccgtg cgctctccca cccagaccga
     8161 cgaccgaata gcgaagctcg cgatcgttca cgtcgagcgt tcgtggccat gtcacggaca
     8221 caggatcgat gccgagcgcg ttcccgtggt agatcgaggc atccagggcg gccgcgagga
     8281 ggttgtgcgc agcttgcacg gcgtggaagt cgccgttgaa gtggaggttg atctcctcca
     8341 tcgggacgac ctgggcgtaa ccgcctccgt tgccgcctcc cttgatcccg aagacgggcc
     8401 ccatcgacgg ctcgcggagg catagcatca cgtccttccc gatcttcccc atgccctgcg
     8461 cgagtgatat cgacgtggtc gtcttgccct cgcctgcctt cgtgggggtg atggccgtgg
     8521 tgatgacgag cttgccgtcc ggccggtcgg ccagccgctc gaggatcgag agggacacct
     8581 tcgccttgga gcggccatac tgctcgagct cgctctcgag gatccccgca tccgcggcga
     8641 catcggtgat cggccgaagg acggcctcct gcgcgatctg cagcccgctc ttcacggcct
     8701 cgcctcccct cggtcccgcc ggctgttccg cggctgcagg aggatacccc ccactcacag
     8761 accgcggagc gaggtccggc gcgtggcgcg ggcgagggga ctcgacgtag gatgcgccca
     8821 gccggcccgc cgaagggccg cccgaaggga ggcgcgagtt ggccaccgac atcgagcagt
     8881 tcgtggagcg gcccgggcgc gacgatgcga tcaaggaggt tcgccggcag atcgaggccg
     8941 aaggcgtgac gtacatgtac taccagttcg tctcggtcac cgggcgcatc atgggcaagg
     9001 gcatcccggc caagcactgg gagacgatcg cccacaaagg cttccagctg gtgtacggct
     9061 cgaccgccaa cctgttcgtc gaccgactcg gcaactacat cggctacggt ccggaggccc
     9121 gggaactcgt cggcatcccc gacgtcgaga cgttctgcgt gctgccgtgg gaccggaaga
     9181 cggcgagggt cttctgcacg ctcttccgcg gacgcgagga agaggtcgac ggcggcgcgt
     9241 tcctgacgtc ggactgtcgg ggcaacctca aacggctcca cgacgagttc accgaggaga
     9301 cggggctgca tctgcgggcc ggcaccgagc cggagatgat gtggctgaag acgaaccccg
     9361 acggcacgcc gtcggtggac ggcaagacca agccgtactg ctaccacatc gaccagttct
     9421 ccgagctcca gccgatcatc cacaaggtga tggagtacgg cgaagccatg gggctcgaca
     9481 tgatccaggg ggatcacgag gacgcgcccg gacagctcga gctgaacttc atgttcgaca
     9541 aggccgaggc caccgcggac cgactcacga cctaccgcca gatatgccgt caggtcgggc
     9601 gggagatgaa cgcgttcgcg tgcttcatgc cgaagccgtt catgggcgtc tccgcgaacg
     9661 gctgccacca caacatctcg ctgtggcagg gcgacgagaa catgttcatg cccgacggcg
     9721 acgatccacg ggtgcccggc aagatcggcc tgcacgcgat cggcggcgtc ctggagcacc
     9781 tccgggcgct cacctgcatc acctcgccga ccgtgaactc ctaccggcga ttcgccgacg
     9841 cggggttctg ggcgccgatc ttcgccgact gggggttcca gaaccggacg actgcgctgc
     9901 ggatctcggc gcccggacgg tacgagtacc ggtccgtgga ctcggcggtg aacccgtacc
     9961 tctccttcgc ggcgctcctg atggcgatga aggacgggct ggcccgcaac ctcgatcccg
    10021 gtcctccgga ggagcggaac atctacgaag cgatcgcgga gggcaaggat gtccaacgga
    10081 tcccggagac cctcggcgag gcgctcgagg cgctcgaagc ggacgacgtg gtgaaggcgg
    10141 cgttgcccgg agacatgtat cgggtgttca tgcactacaa gaccgacgag tggaagcggt
    10201 tcatcgggac ggtcagcgat tgggacgtcg ccgagtacct ggacgtgctt ccctagagcg
    10261 agaggatcat aggaccaaga ccagaggaaa ggagctcttc cctccatgtg cggcatcgca
    10321 ggcatcatcc atcgcgacgg ctcctccgac gtggggagcg agatgactcg catgctccag
    10381 gcgatgaagc accgtggccc ggactcatcc ggatacgcgc tcttcgggga gccgtccgag
    10441 ctcgtcgtga tccgcttcaa gctcgccgat gcgaacgacc cgcgcgactt cgacttcaag
    10501 gagcgcctcg aacgtcaccg ccaggaggtg gagtcccgtc tgcacaagat cggcgcctcg
    10561 gtggaccgcg tggagcggga gacggagtac gcctatcggg cgacggcgcg ctacgacggc
    10621 gacatgaaag cgctggccga ctacctcgaa gacgtgccgg ggtgcgaggt cctctcgctc
    10681 gggcattcgc tcgagatcgt gaaggatctg ggcgacgccc aggaggtctc ggatctctac
    10741 cggctcggcg ggttcaacgg cacccacgcg atcgggcatg tgcggatggc cacggaatcc
    10801 gacgtcgaca tctccggcgc tcatccgtac tgggcgtacc cgttctccga cgtggcggtc
    10861 gtccacaacg gacagttgac gaactacttc cactggaagc gccgcctcga atccagtggc
    10921 caccggttcc agtcggaatg cgactccgag atcatcgcgg tctacctggc ggagaagatg
    10981 gcggacggcg actccctcga agacgcgatg cacaagagtc tcgatgagct cgacggcgtg
    11041 ttcacgtaca tggccgtcac gcaggacgcg ctcggagtgg cgaaggacga gatggccgcc
    11101 aagccgctcg tcctgtacga gtccgacggc atcgtcgcga tggcgtcgga ggagatcgcg
    11161 atccgcgcgg tgttggaccg tgagatcgac acgacggatc cctacgaagg gcaggtgctc
    11221 gtgtggacac gatgagcggg gcccggcgca ccacgtacga cgcgcgcggc ctcgcggagc
    11281 cgcacgctga ggttccgaag gtctccgaca tcgacggcac caccgcgagc ttcgacgcga
    11341 cggacctcac gacgcgccag atcaacctgg agctccgctg gctgctctac gagcagggga
    11401 tgcgcgacgt caccgtgcgg aaccccgggg cgaagcactc gatcggcgtc gggatcctgc
    11461 agcgctgccg gatccgctac gaaggttccc tgggctactt cgggctcggg ctgatcgacg
    11521 ggccggagat cacggtgacc gggcgggtcg gctggtccgt gtgcgagaac atgatgtccg
    11581 gggtcgtggt gatcgagaag aacgcggggt ccctcacggg cgcggcgatg cgcggcggtg
    11641 acctgctcgt gcatggaagc gtcggggccc ggaccgggat cgaccagaag ggcggcacga
    11701 tcatcgtcgg aggcgacgcc ggaacgatga ccgcgttcat gatgcagcgc ggccgccaga
    11761 tcatcctcgg cgacgtgggc cctggcctcg gcgactccat gtacgacggc gagatctacg
    11821 tcgccggatc ggtgaagtcc ctcggcatcg actgcgtcga gggggagtgg accgacgctg
    11881 acaccgagct gatcgaacgc aagttccgga tctacggcct cgggagcccc cccgcgttcc
    11941 agaagttcgt gtgcggcaag atgctctaca actacgacaa cctcgagccg agcgagaaga
    12001 agctcgtcat ctaggagagg ggagagagcg agccgatggc tgaggtgtcc tcccgcaagg
    12061 aggtcctggg gcgcagcccg atcttcaccg aggaggtcat caacgacatc cacgtgaagg
    12121 cggagctcgg gcgataccgg atgcgcgggt tctcgatgtt caagccgatg cccggttggg
    12181 accagctgat gttcctgcca ggcacgctga cacgcttcgt gatcgagggt tatcgcgaga
    12241 agtgcgagac gaagacggtc ctcggcgccc gcttcgcgaa gcatccgatc gagctcgaca
    12301 tcccgatcta catcaccggg atgtcgttcg gcgctctgtc gctcgaggcc aagatggcgc
    12361 tcgcgaaggg cgcgtcgatg gccggcacgg ccacctgttc gggagagggc gggatgatcc
    12421 cgccggagcg ggacctctcg acgaagtggt actaccagaa catccagtcg cggtacgggt
    12481 tcaacccgca ccacctgatg ttggcggacg cgtgcgagtt cttcatcggt caggggtgca
    12541 aggtcggcct cggcgggcac ctgatgggcc agaaggtgac cgagcaggtc gccgagatgc
    12601 gatcgctccc cgccgggatc gatcagcgct cacccgcccg ccatccggac tggctgggac
    12661 ccgatgatct ctcgctgaag gtgcaggagg tccgcgaggc gaccgactac cggatcccga
    12721 tccagttgaa gctcggcgcc gctcgcgtgt acgacgacgt gcggatggcg gcgaagtgcg
    12781 gcccggacat catctacctc gacggggccg aggggtcgac cggcgcgggg ccgcacatcg
    12841 cggccgagga gacggggatc cccctgatgg ccgcgatccc ggaggcgcgc cgggcgctgg
    12901 aggacgtcgg cctcgcggac gaggtggacc tcgtggttgc cggcgggatc cgcaacggcg
    12961 ccgacgtcgc gaagtgcctc gccctgggcg cgacggcggt ggctatcggg cactcggcgc
    13021 tgatggccct gaactgcaac aaggagatcc cgggtgtcac cgactacgag ggtacggtcg
    13081 gcgtgccggc cggccagtgc taccactgcc acacgggtcg ctgcccggtg gggatcacca
    13141 cccaagaccc ggagctccgc aaacgcctcg aggtcgactc cgccgccgag cgcgtctaca
    13201 acttcctgac cgcgatgacc atggagctcc agatgctcgc ccgcgcgtgc ggcaagacgg
    13261 acgtgcactc gctcgagccg gaggatctct gcgcgctcac cgtggaggcc gcagctatgg
    13321 cgaaggtgcc gctcgccgga acggactaca tccccggcgt caccgacaag gagacgatgg
    13381 aggacatcaa ggcgatgatg cagaagtacc tcgccgacac gggccagttc ccgggctgag
    13441 gcgagagatc gggggatcag cgatgaccga gccccatcac ctctcgacga agatcgggat
    13501 cgacgcggag gagctcgccg gcaggcggtt cccgtaccag gaggacatct ccctcgtgga
    13561 ggatctcgac ctcgacgagc tgacgccggg cgaggacctg aactggctcg aggacgtcac
    13621 gctcctccag gaggacggcg tcgccgccgt cttcgaccgc tactcgaact ccttcctgaa
    13681 gatccacttc gagatccccg ccggacgcga ggacgagctc gctcgcaagg tgctgatgaa
    13741 gcacctgatc gagggcaact cgtacggcat ccagttgaag aagcagcacg cgaagttcgc
    13801 acagccggag ctgggaccct gggtcgaggg gtccgagacc gtcggcaccg actggaagcc
    13861 ggccgtgatg gagggctggg agcggcccgc cttgcactga ctggctcctg tcgttcccat
    13921 gacggtacgc cgcccgctga tcgcgatctc ggagggcttc gccgactacg gcgactacta
    13981 cggctacggg tacgggcgcc cgctgctcgc agcgggcgcc ctgccggtcc tcctcccgta
    14041 ttacgaacgg gaggaggacc ggttcgacct gatcgagcgg atcgacggcc tggtcctggc
    14101 cggcggccga gacatcgagg cttggcggta cgggcgcgag gagccgcatc cccagcatct
    14161 gccggggcag ccgttcctgg acgagatcga cctctcgtac gccctgctcg ccgtggagga
    14221 cgacgtgccg gtgctcggcg tatgccgagg atgccaggtc ctgaacgtcg cgctcggcgg
    14281 gacgctctgg ggtgatctcg gggagttccc gggcggcggg gcggaccatc ccggcgcgaa
    14341 gtgggacgag tggcgggcat tggtggcggc gacgctcgag ggaggagcgc gccccgcaca
    14401 tccgacccac ccgatcgagg tgcggcccgg gtctctgctc gccgcgcacc tcggtgagcg
    14461 gtacgaggtc gacagctacc accaccaggc cgtgcgggag ctcgggaccg gtctggaggc
    14521 cgtcgcctgg gcaccccacg gcacgatcga ggcggtcgag atgccgggat cacccgcctt
    14581 cgtcctcggc gtgcagtggg agctgcacga ggagtggcag gacgacgagc gcatgctcgg
    14641 gatctgggag atgttcgtcg aggacgcgcg ccggcggatg gaggagcggg ccgaggtccc
    14701 ggtgacgtga ccgcatcgat cgaggtcacg atcgacgcgg tcgacggtcc cgccgcggtg
    14761 gcgttctggc agcaagcgct gggctacgag cgtcgatcgg cgcggggagg ttacgtcgtg
    14821 ctcggtccac ccgccgaaga cccgcggccg gccgtcgtga tccagcacgt ggacgccgtc
    14881 tcgccgggca agacccccgt ccatctcgac ctccgcgtcg acgatgtcga cgcggaggtc
    14941 gcccgtctcc aggcgctggg cgccaccatc ggatggacga tcgatgaaac cgcggaaggc
    15001 gggagccggt ggaccacgat gtcggatccg caggggacgc tgttctgtgt gtgcaccgcg
    15061 agatcgtagg atccctgcgg atcggaaccc gaaaggcgga cgcagatgac gctcccggag
    15121 cacgcccgct acgtcgtgat cggcgcgggc atccacggtc tctcgacggc gtggcatctg
    15181 gcgaaggagc tcgagtcccg tgggcagggg gacggccggg acgtggtgat cctcgacaag
    15241 acgggcgtgg gcgccggtcc gtccgggatc gcgtgcggcg tgatccgcaa caactacttc
    15301 cagccggcga tgcgggagct gatggcgcac tcggtggcca tctgggagtc ggatcccgag
    15361 gccttccact accacccgat cggctacctc cagatcgccc cggaggtgat gcgggccgat
    15421 gtcgccatga tccacgagca acagaaggcg atcggctaca cctcggagct gatcgagggc
    15481 gaggacgatt gccggaggta catggagtcg atcttctcgg actggcaggc gaggaacatc
    15541 accgcggtcc tgcacgagaa gaagggcggc tacgcggaga acttgccctc gatggagggc
    15601 ctccgaagca agagcgaggc gctcggcgtg gcactccacg ctccggtctg ggtcaccgga
    15661 ttccgcaggg acgggggagt ggtgagggcg gtcgagaccg accggggagc gatcgagtgc
    15721 gagcaggtcg tgatcgcggc ggggccctgg gtccgtgact tctggcacat gctcgacctc
    15781 cccggcgaga tcgacgtcct cggtcgcgac gggaaggtct acgaacggcc gatgtggacc
    15841 tactggtgcc tgcaggaagg cacgctcggc gtcgatccga cgtacctcct cgacaacgag
    15901 gggaacatgc cgcccgtcgt acacatcgac acggacgcgc cgctcgtcga cgacatggac
    15961 ggctcgatcg tgaccgagga tctctggggc atctactaca agccggacgt gaacttcggc
    16021 ggcgtacagg gtggggcgat gccttgggtc gtggagcagc ccgcggacga ggtggccgtg
    16081 gatccgtacg gacacctcag cacggagttc gtcgtgaccg cggagttcga gcggatgtgg
    16141 acgtccgccc tcgcgcattg ccacaagcgg ttcgaaggca agcggccgct ctacaagaag
    16201 gcgcccaccg gtgggatcgg tgcgttcacg cccgacagct tccccgtgtt cgatcggttc
    16261 gtggacaacg cgtacgtcgt cgccgactcg aaccacggct acaagatgat cgggatcggc
    16321 cagctcgttg cgaaggagct cctcggggag caacaggcac tgctcgagcc gttccggttc
    16381 tctcggttcg ccgagggtcg gctccacccg gtcagccaat cgccctatcc gtggagctga
    16441 gttgaccgat cagcccacca cacgggcgct cccggtcgag ttcgacctcc cggtccacgg
    16501 cggcttcgcg ccggcgggga tcggcgagac gttcccggac gatgcgcccg acgcggagga
    16561 gctcgagatc gcgggagagc ccggccaaga tccggtcgcc gccttccggc ggaccctggg
    16621 gatgtttgcg accggtgtga ccgtgatcac gacccagtcc ggcgaccagg tccacggcat
    16681 gaccgcgaac gcgttcatgt ccgtgtccct ccggccgcca ctcgtggtga tctccgtcga
    16741 ccgacgggcg aggatgcact ccctgctgaa cgagagaagc cgatacggta tcagcgtgct
    16801 cgcggacgag cagcgttcgc tctcggaccg gttcgccggc agaggtgtgg gcgacgacca
    16861 agaggtcgag ttcgacgtcg tccacgagac gccgttggtg cgaggagccc tcgcgcacct
    16921 ggtcgcccgg gtcgtccgct cgtcctgggg aggcgaccat tcgctcttca tcggacaggt
    16981 ggagtacgcg cggtacgggg aagggcagcc gctcctcttc cacgggggcc ggtatgagca
    17041 tctgctcgag cgcgctccgg tcttctcctc gctgccgccg gagatcctcg ccgccatcac
    17101 ggccgagggt gatgagcgga tgttcgagcc cggcgagttc gtcgtccggg agggcgagac
    17161 cggtgacgag ctcttcgtga tcctcgaggg ggaagctcgg gtcgagcggg cgagcaacgt
    17221 cctcacgatg ttccgggatg gggacttctt cggggagatc tcggtgttcg acggacgccc
    17281 ccgtagcgca gacgtcgtcg ccgcgacgcc gctccggacc ctcgcgatct cccgtgagct
    17341 ggtgcgagcg gcgctcgagc gagagcccct cgccgcatgg gcgatgctcg aggtgctcgc
    17401 cggccggctc cgcgacgact gacgcggctc ggtcgtcccg gcgctacagg cggtagggaa
    17461 ccgcggacac gaccacggag ggctcgaaca ggaacgtgcc cttgagggtc atcgccgtgt
    17521 ggttgtgcag caggcgatgc caccacttgc gcaccacgaa ctcggggatc acgagcgtca
    17581 cgaacgtctt cgggtgctcg tcgaggacct cccgcaccca cgcgtggatc ggcccgccga
    17641 cctgccggta gggggaatcg agtacctcga tcggcacgtc cggatagcgc tcctgccaac
    17701 gggcccggag ctcctccgac gccttcccgt cgacgtccac gtggagacat cgcagctcgt
    17761 cggctcgcaa cgacatcgcg taccggaggc tccggtcggt ggcgttgtgc gcacccgatg
    17821 ccagcacgac ggccacgtgg cgccatccct ccatcgcgcg ccggacgtcg ccgtcggcgg
    17881 tcactggctc gagcgagtcg tagccgggat gatgcgccac gttcgtgacc acgacatcgg
    17941 cttccgccac cagggcggcc ttgatccgct ggacgaggag cttgtggagg aagtggcgcc
    18001 atccccggta ccgaacggat tccgggatga tcacgttgat gagggcatcc tcgtccgcgc
    18061 cggcgcggat ctcacgcgcg aggttcctga tctcggccgg gatgctccgc gtgcgcaagc
    18121 ggagctcgag catctcgccg agcctgcccc agctgtccca catgaacgcg atgtccgtgc
    18181 ccgtcggcgc gaagtgtacg agccgcacct cgagcgggaa cacgcgtttc gcgtacgccg
    18241 tcgcgtagac gtcctcaggg ccgggtgtcc gtacggggat cacgacccgc tggcgtggga
    18301 gatctcgcac ggggccgcgc cgcccgggat ccacgagcgc cgcggccgtc tcttcgtaat
    18361 ggtggttcac cctgagcaac ccgaggagca tcacggggat cgcgatcagg atgatccacg
    18421 cgccatccgc gaacttggtc gcggcgatga tcacggtcat cacggcggtg acgaccgcac
    18481 cgaacccgtt gatcgcgagg cccttcctcc agccctgctc acggagggtc gtgtgccgct
    18541 tcgccatgcc tgcttgagag aacgtgaacg acgtgaagac cccgatcgcg tacagcggga
    18601 tcaggttcgt cacgctcgca tcgaacatga ccaggaggag cgaggcgaag atagacagca
    18661 cgatgatccc gttcgagaac acgagacgat cgccgtagcg cgtcagctgg ctcggtagga
    18721 agtggtcgcc ggcgtggaag ctcgccaggc gcgggaagtc cgcgtagctc gtgttcgctg
    18781 cgaggaccag gatcagcatc gtggcgacct gcacgaggac gaagagcacg tcgccgaccc
    18841 ccgaatcccc gaacacggct ctcgcgatct gcgcgttcac ggtgaccttc tcgtgctcgt
    18901 ccggaacgat ctgcatccgg gcggcgagga tcgagagccc gaggaacatc gcgcccagca
    18961 gcgtgcccat ccacatcaac gtggtgatcg cgttcttcca ctcgggtggc ttgaaagccg
    19021 gtacgccgtt ggagatcgcc tcgacgcccg taacggcggc gccacccgat gcgaaggcgc
    19081 ggagcaccag gaagacgagc gccgctccag agatcgagac cgtctcgggg acggggaagg
    19141 ttgacggcga ggtcgggagg gtccccgcga acagacggaa gaacgacacc cccaggagaa
    19201 cccccatcat caggaggaag aagtacgtgg gggcggcgaa gaggcggcca gactcacgga
    19261 cgccgcggag gttcccccag gtgaggatga ccacgaaccc gatcgcgatc cacacgcgcc
    19321 acgggtggag cccttcgtag atcgaggtga gcgccgccgt gcccgcggcc acggacaccg
    19381 agacggtcag gacgtagtcc gtcaggagcg cgaccccggc gacctgcgcc ggcttgaccc
    19441 cgaagttgtc ccgggtgacc aggtaggccc ctccggcttg cggatacgca cggatcgtct
    19501 ggcgatacga gaacaggagg atcgcgagga cgatcaggag ggcgatcgtg atgggaacga
    19561 ctttggcgaa cgccaccgtc ccgagaccgg cgaacatcag gacgcggacg atctcctccg
    19621 tggcgtaggc ggacgatgac aggttgtcgc tcgcgaagac cgcgagggcc gtcggcttgc
    19681 ccaatcgttc gtggatgagt cgctcggtcg ccatgggagc tccgacgatg cgacgcttga
    19741 accgggaggc gcgatccgag cgttcgggac gcgccggacg ttcgatcgtc tcctcagggg
    19801 tccgcgtctc ggactgtgcc tgcttcatgc cggcagtttc ggatcccgct cgtcagcgat
    19861 cgctcaggat gccggtggtt cacctcaaga tgtcctcaag atgcgttcgc ggtcgcctca
    19921 gggtcccgag aggggtgtgc gagactccga gacatgagga aggtccttgc ccctcggccg
    19981 gcctcgatca ccgccatcct tgcgatcggg atcctcgcat cggcctcagc cgtggggatc
    20041 gcgctcgcca tcggttcgaa gaatctcgcc gccgccaccg ctctctgcct cctcggagtg
    20101 gtcgccgccg gcgcggtcgc cggccggggc agcgcgatcg tggcctcgtt cctgtccttc
    20161 ctcggcctca atttcttctt caccgagcca catcacacgc tggcggtcag acacgcgtcc
    20221 gacgtcgtcg ccctcgtcgc gttcctcctc tcggggctca tcgtcggcgc cctcgtcagc
    20281 agggtgcgtg aggaacggtc tcgagcggaa cgtcgggcaa ccgaggcgct cttcctcaat
    20341 cgaaccaccg agcgattcat ctcgacggag tccatcgatc gcaccctcgc cggactggcg
    20401 gaggcactcc tcggcttctt cgggctcgtg cgctgcgaca tctctacgtc ggtcggcgtc
    20461 gggagggccg ggaccgacga tccgggaccg gtcgtgggtc agacgatctc gaccccgctc
    20521 gtcaccccat cggggtcgtt cggtgcgttg accgtggtgc gcgcggccag cgaggatccg
    20581 ctcgcgccac ctgagctcgg actcctgcac acgctggcgg cgcagacggc attggcgatc
    20641 gaacgggcgg cgctcgatgg tgaggtgagg gcggcgcgga tcgaagcgga ggcgcacgcc
    20701 cttcgagcgg cactcttctc ctcggtgacg cacgacctgc ggacgccact ctcgtcgatc
    20761 aaggcgagcg catcggggct gctcgccgaa ggggcccatt acagcgacga agagcgacgc
    20821 gacgtgctcc gcacggtcgt ggaggaagcc gaccatctga acctcatcgt cggcaacctc
    20881 ctcgacctcg cgcggatccg cgccggggcg ctcgtcccct cgaagcagac gatcctcgtg
    20941 gaagaggtga tcggatcggt cctgcagcgg atgcgacgat cgctcgaggg tctcgtggtg
    21001 cgcaccacga tccgccccga gttgccgccc gtcgacgccg atccggtcca gatcgggcag
    21061 gtgctctcga acatcatcga gaacgcgatc cgcttctccc cgaatgggag cgagatccag
    21121 atcacggccg ctcgctggca ctcggcggtc caggtgcgga tcacggatca gggtccgggg
    21181 atccccgcgg aagaccggga tcgggtcttc gaggagttct atcgtcggga cgccggcgcc
    21241 gggcgcgggg ggacgggtct cgggctcgcg atcgctcggg cggtggtcgt cgcgcacggc
    21301 ggcacgatct cggccaggtc ggctccgggc ggcggaacgg cgatcgtctt cgagctaccg
    21361 atcgccgtcg gagagacgca cgacgaagcg tcccgcgcgc ctcggagcgc cgtgtcatga
    21421 cgcgcgtgct tgtgatcgac gacgagccac agatcctcag ggcgctccgg cgatcgctcg
    21481 aggcgcagga ctacgaggtc gcgacggccg aggacggcga agcgggccta gcactcgccg
    21541 cggcgcggac gcccaacttg gtcgtgctgg accttggctt gccggacctc gacggcacgg
    21601 aggtgatccg gcggctccgc tcgtggaccg acgtgccggt gatcgtccta tcggtccgtg
    21661 aaggacgctc cgacaagatc gatgcccttg atgccggagc cgatgactac gtgacgaaac
    21721 cgttcgacgt ggaggagttg ctcgcgagga tgcgggccgc gctccgccgt gcggatgcgg
    21781 acctggccgt cgagccggag gtgcggtacg gggatctcac gatcgacctc gctcgccggc
    21841 tcgttcgact cggcgacgcg atcgtccacg tgacaccgaa cgaatatgcc ctcctcgccg
    21901 cgatggtgac gcacccgggg aagctgctca cccaccagtg gctcctgcgg gaggtgtggg
    21961 gtcgcggcta cgcgaacgag agccactacc tgcacgtcta tgtgcgacag ctccggcaga
    22021 agctcggcga cgacgccgcg atgcctcgtt tcatcatcac cgaacccggc gtgggctatc
    22081 gctggaaccc ggacgcggcc gtcgaacggc gctagtcgac cgcttcctcg atcggttcct
    22141 cgatcgctgg aagatcggag agccagctcg tgcggtggcc ggtctcgtcc cagaggatcg
    22201 ggaagaagga ctcgtccatc gaggtgtcgc cacgccgccg gtatcgcgag tagatcaggt
    22261 cgacgttgcg gtggtggaag cgcgcgtgcg agggaagcat gtgcgagacg agggccatcc
    22321 gcgccacggt gccgctcgtg ttcggctcgg agccgtgcca cgcgaggttg tgatggaagg
    22381 caccgccgcc gggtctcacg acgaccggca cgatctccat ctccgcgtcc gccggtttgg
    22441 catcccggac gccggcgagc cagtcctccg gcgcgtggaa ctgtgaccgc tcaggcggcg
    22501 tccagggcca gtggttggac ccgcgcacgt acgcgatcgg accggcatcg gcctgcgtct
    22561 cgtgcagcga catccaacac gtgaccatct ccggaggcac gaggtagtcg gcgtacgagc
    22621 cgtcctggtg gaagccgatc gccttggtcc ccggcggctt ccagagcacg ttgtcctgca
    22681 ggatgcggac gccctggtag ctcatcagct gagccgcgag ccggccgatc ctcgcggaca
    22741 ggacctgggc ggcgatcacg gagtccgcac gccaggcgtt gcagagctgt cgcgtgcggt
    22801 cctcggggtc gcgccccttc acccagttca cctcgtcggg cttgatcccc gtctcgtact
    22861 cgccgtcgaa gagccggagg tagcgctcct cgaggagatc gagagccttc tgggagatca
    22921 ggccctcctc gacgatcagg aacccgtcac ggtggaacgc ctcgacctgg tccggagcga
    22981 acgattcgag cggcggagcc atcgtcagga gcgcggccgg gtcttgtccg ggtcgtagaa
    23041 cgggaagcgg acgaccttcg cggcgatgcg cttctgctga ccgtcgagct tgcccacctc
    23101 cagatccgtt ccgagatccc ggtgctggat cgcaacccgt gcgagcgcga cgttcttctt
    23161 caggatcggg gatcgcgtcc cgctcgtgac gacgccgact tgctggcggc cggcgtagac
    23221 gccgtcgccg tgcgcggccg gctcgttgcc gtcgagctcg agtccgacga gcgcccgttg
    23281 tggatgcgcg cgccgctccg cg
//
LOCUS       DASUYD010000233         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1278188, whole genome shotgun sequence.
ACCESSION   DASUYD010000233 DASUYD010000000
VERSION     DASUYD010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278188"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF195_12350"
     CDS             <1..297
                     /locus_tag="VF195_12350"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581647.1"
                     /translation="GLATTAVVPNADAAPAVVNAIILPLLFLSGIFFPLEGAPQWMIV
                     LGDVFPVKHFAEAMLGSFYGPPLQFEWVDVLVVAAWGVVGLALAIRFFSWEPRK"
     gene            378..1295
                     /locus_tag="VF195_12355"
     CDS             378..1295
                     /locus_tag="VF195_12355"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581648.1"
                     /translation="MRKVLSLLAVGSLLFAACAEDNPTIGGTGTTATGGTGATTPATA
                     ADCAAANTDAFLSPGTLTVGTGNPAFPPWWEGGTTDGSEFEFNDPALGEGYEGAVTAE
                     VATRLGFSFPDQVTFVPVGFNKSFAPGDKDFDFVLQQISYKPERDDAVDFSDSYYNVN
                     QALVSAQGTSVADATTIEELKEAKLGAPIGTTSLDVITEQIQPTQEPAVYDDLAQAVT
                     DLQNGDIDGIVVDLPTAFFMAAVQIDKGVVVGQFGTESDEYFAMAFDTGSPLVECVNL
                     ALAEMTEDGTLDAIEEQWLADKTNAPVLD"
     gene            1303..2202
                     /locus_tag="VF195_12360"
     CDS             1303..2202
                     /locus_tag="VF195_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020386406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6581649.1"
                     /translation="MIGGQAPGEFEGLPGGGSTPPTGDRGSKILAPLKTEGVRGVAIA
                     VVSTVVVFALIAFAVLTSSGWPVIKEAFFDPTIFWDSLSKIMGKFGRNVFIFVVAEIF
                     VLILALILAVMRSLRGPVFTPLRILAAAYTDLFRAVPTILVILLLGFGAPSLQIKGVP
                     HSDVFWAIVALVLVYSAYVAEVYRAGIQSVHPSQVASARALGLSRGKTMRFVVVPQAV
                     RRVLPPLLNDFIGLQKDSALVAVLGYTEALRQAQIISAAEFDFTPYVALALVYIVITI
                     PEARFVDWLVARDQRRRQAAGGV"
     gene            2204..>2487
                     /locus_tag="VF195_12365"
     CDS             2204..>2487
                     /locus_tag="VF195_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015805658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6581650.1"
                     /translation="MSETAALAIEGLHKSFDGLEVLKGIDLAVREHEVVCVIGASGSG
                     KSTLLRCINLLEPVNAGRVLIEGEEITARGVDVNRIRQRVGIVFQAFNLFP"
BASE COUNT          361 a          834 c          856 g          436 t
ORIGIN      
        1 ggcctcgcga ccacggccgt cgtcccgaac gccgacgcgg cgccggcggt ggtcaacgcg
       61 atcatcctgc ccttgttgtt cctgtcgggg atcttcttcc ctctcgaggg cgctccgcag
      121 tggatgatcg tcctcggcga cgtcttcccg gtgaagcact tcgccgaggc gatgctcggg
      181 tctttctacg ggccaccgct ccagttcgag tgggtggacg tgctcgtcgt ggcggcgtgg
      241 ggtgtggtcg ggctcgcgct cgcgatccgc ttcttctcgt gggagccgcg caagtgaggc
      301 cgccgatccg ccggtctgcg taggacagag cgtgtgctag tctccgcgga ccaccaggcc
      361 ggccaagggg ttccacgatg cggaaagtgc tctcgttgct cgcagtcgga tcactgctgt
      421 tcgccgcgtg cgccgaggac aacccgacca tcggcggcac ggggacgacg gcgaccggcg
      481 ggacgggtgc gacgacccca gccaccgccg ccgactgtgc ggccgccaac accgacgcct
      541 tcctgagccc cggcaccctg acggtcggca cgggcaaccc ggccttcccg ccctggtggg
      601 agggcggcac caccgacggc tccgagttcg agttcaacga ccccgcgctg ggcgagggct
      661 acgaaggcgc cgtgaccgcg gaggtggcga cccggttggg cttctcgttc cccgaccagg
      721 tgacgttcgt gcccgtgggg ttcaacaagt cgttcgcgcc cggtgacaag gacttcgact
      781 tcgtgctcca gcagatctcg tacaagcccg agcgcgacga cgcggtggac ttcagcgact
      841 cgtactacaa cgtgaaccag gcgctggtgt cggcccaggg cacgtcggtc gccgacgcga
      901 ccacgatcga ggagctgaag gaagccaagc tcggcgcacc gatcggaacg acgagcctgg
      961 acgtcatcac cgagcagatc cagccgacgc aggagccggc ggtctacgac gacctcgcac
     1021 aggccgtcac cgacctgcag aacggcgaca tcgacggcat cgtcgtggac ctgccgaccg
     1081 cgttcttcat ggcggccgtg cagatcgaca agggtgtcgt ggtcggtcag ttcgggacgg
     1141 aatcggacga gtacttcgcg atggcgttcg acaccggcag cccgctcgtc gagtgcgtga
     1201 acctcgcgct cgccgagatg acggaggacg ggacgctcga cgcgatcgag gagcagtggc
     1261 tggccgacaa gacgaacgca cccgtcctgg actaaccgct ccgtgatcgg cgggcaggcg
     1321 ccgggggagt tcgagggcct gcccggcggc ggttccaccc ctcccaccgg cgaccgcggt
     1381 tcgaagatcc tcgcaccgct caagaccgaa ggcgtacgcg gggtggcgat cgcggtggtc
     1441 agcaccgtcg tcgtcttcgc gctgatcgcg ttcgcggttc tcacgtcctc cggttggccg
     1501 gtcatcaagg aggcgttctt cgatccgacg atcttctggg actccctctc caagatcatg
     1561 gggaagttcg gtcggaacgt gttcatcttc gtggtcgccg agatcttcgt cctgatcctg
     1621 gccttgatcc tcgccgtgat gcggagcctc cggggcccgg tgttcacgcc gctccgtatc
     1681 ctggcggccg cgtacacgga cctgttccgc gcggtcccga cgatcctggt gatccttctg
     1741 ctcgggttcg gggcgccgtc gctgcagatc aagggcgtgc cgcactcgga cgtcttctgg
     1801 gcgatcgtgg ccctcgtgct cgtgtactcg gcctacgtcg cggaggtcta tcgggccggg
     1861 atccagtccg tccacccgtc gcaggtcgcg tcggcgcgtg ccctcggcct cagccgcggc
     1921 aagacgatgc ggttcgtcgt cgtgccgcag gccgtccgtc gcgtgcttcc gccactcctc
     1981 aacgacttca tcggcctgca gaaggattcc gcgctcgtgg ccgtcctcgg gtataccgag
     2041 gcgctccgtc aggcgcagat catctcggcg gccgagttcg acttcacgcc gtacgtcgcg
     2101 ctggccctcg tgtacatcgt gatcacgatc cccgaggccc ggttcgtcga ctggttggtc
     2161 gccagggacc agcgtcggcg ccaggccgcc ggaggggtgt gacgtgagcg agacggccgc
     2221 gctggcgatc gagggcctac acaagtcgtt cgacgggctc gaggtcctca agggcatcga
     2281 cctcgcggtg cgagagcacg aggtcgtctg cgtgatcggc gcgtctgggt cgggtaagtc
     2341 gaccctgctc cggtgcatca acctgctcga acccgtgaac gccgggcggg tgttgatcga
     2401 gggtgaggag atcacggccc gcggggtgga cgtgaaccgg atccggcagc gcgtggggat
     2461 cgtcttccag gcgttcaacc tgttccc
//
LOCUS       DASUYD010000234         7119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_177468, whole genome shotgun sequence.
ACCESSION   DASUYD010000234 DASUYD010000000
VERSION     DASUYD010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7119
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177468"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /gene="infC"
                     /locus_tag="VF195_12370"
     CDS             <1..597
                     /gene="infC"
                     /locus_tag="VF195_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011027874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX6581651.1"
                     /translation="EPRLNDRIRSPQVRLVGADGEQIGVVATQEAVRRARELDLDLVE
                     VAPQANPPVCRIMDYGKFKYERDIRQKEARKKQARTELKEIKFRPKIDAHDYGTKKGH
                     VERFLRGGHKVKVTIMFRGREMTHTELGRKILDRLVGDLGEMVTVESMPKQEGRNMIM
                     VISPNKRYMEQLARARARESAPVIDAGAVATAAETDEG"
     gene            601..798
                     /gene="rpmI"
                     /locus_tag="VF195_12375"
     CDS             601..798
                     /gene="rpmI"
                     /locus_tag="VF195_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX6581652.1"
                     /translation="MPKNKSHRATAKRFRITRNGKVLHRKATGNHMLTKKSSSRRRRV
                     EGMAEVGSKADAKTVKRLLGD"
     gene            802..1167
                     /gene="rplT"
                     /locus_tag="VF195_12380"
     CDS             802..1167
                     /gene="rplT"
                     /locus_tag="VF195_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006553977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX6581653.1"
                     /translation="MARIKRSVHAKKKRREILEAAKGYTGSRRKRLRVAKEQVMHSGM
                     YAYRDRRDRKAQFRRLWITRINAAVRPHGLSYSRFVSGLHAAGVEVDRKILADLAVRD
                     PEAFAQLVATAKASGPSDF"
     gene            1225..2661
                     /locus_tag="VF195_12385"
     CDS             1225..2661
                     /locus_tag="VF195_12385"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HEX6581654.1"
                     /translation="MTSGRNPKVVAAVRLKKRALREADRRFLVEGAQAVAEALAEDGR
                     LESLFVEDDLDPLAIRAKQAGVTVDHVTERVMERLTSTITPQGVVGIAPFVDVTLDAL
                     GPPGAVALLHEVRDPGNAGTILRSADAAGAVGVVFAGSSVDPYNAKSVRASAGSIFHV
                     PVVRDVATGDAIATLRDRGFTIVAMDMHGDEDLFEAQLPPSSAFVFGNEAHGLPAEIL
                     GTAERRVRVPQAGRAESLNLAAAATICLFEWVRRSRATTGTLEALVAAAAHDIRSPLT
                     AMKGFGYALSKRWTDMTDEQRALMLTGIVHDADRMDTIVRQLLDAARVMSGTFQSFAE
                     LVDVSDVVRQITEQLARDPEHPQIEWSGGAGAKAMLDGGRLKTTLLAFTEALVWWASD
                     GPIRIESELWGGRVVVSASREAHGDVDVASLFEARRPGAGRGSKIGLYVARRVAEAQG
                     GSVSGDLADGRLTLHVELPATGPEPLAP"
     gene            2675..3733
                     /gene="pheS"
                     /locus_tag="VF195_12390"
     CDS             2675..3733
                     /gene="pheS"
                     /locus_tag="VF195_12390"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX6581655.1"
                     /translation="MDVQHMRESVEEVRERGRRVFAEASSREELEAAQVTVLGRKSLL
                     AEVQRSLGALEPEERKDLGRIVNEVRAVLESAASERREELESTAEAALVTADRLDLTL
                     PGRRPRTGSMHPFTLIEHEIVDIFTRMGYRVIEGSEVEDEWHNFDALNIPPDHPARTS
                     MDTTFVDVPGHPELVLRTHTSPMQIRTLESQRPPVAVVVPGRVYRNEEITARNMPVFH
                     QVEGLLVDEGITFADLKGTLEVFYRALLGEEREIRLTPSFFPFVEPGAQVDVSCFVCD
                     GAGCKTCSYSGWLELMGSGMVHPVVLENCGLDPERYTGFAFGGGYDRLAMIRFQIPDI
                     RLLWDPDVRFLEQFEAAG"
     gene            3733..6099
                     /gene="pheT"
                     /locus_tag="VF195_12395"
     CDS             3733..6099
                     /gene="pheT"
                     /locus_tag="VF195_12395"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX6581656.1"
                     /translation="MRVPVGWLRERCPTDLSPEELSDLLALKGVHLESIERPWEGLAG
                     VVVARVLEVRDHPNAEKLCIAIVDGGDGPTQVVVGIRNMKPGDLVPWARPGARVPVLD
                     RPLEAKPLRGEISNGMLCSPRELAVSHEHETGIMLLPEDLTVGADLRSTLGLDDVVLD
                     LEIESNRPDLLSITGIAREVAGATGEPLIRPDITVAEVGEPASTVATVEIRDLQGCPR
                     YLARIVRGIGPGRTPILVQARLTACGVRPISPVVDATNHVMLDLGQPLHAFDLQRLAG
                     PGIVVRRSEPGERLETLDGVGRELDDDLLICDLERPVAIAGVMGGASSEVDPGTVDVL
                     LESAYFEPRSILRTSRRLQLLTEASVRFSRGTDPEGVGPAAARAARLIAAWTGGAVLE
                     DAVDVGGPPPRRSIGLRSSRAAALLGYPVSAAAAAQALAKIEIAAEPTDDRVGVEVPS
                     FRPDLEREEDLIEEVVRIQGFDRLPSTVPALREVGSEQASYRTRRRVRELLVRAGVRE
                     AASLTFASAAEVELMRHTDPVRVANPPSAEEPFLRTSLLPRLLEAASRNQQRGEISIA
                     LFEIGHVFRLADPVDEREHLGILLAGRAGEGLHAEDRGWDALDAKGILDVVLGGLGVG
                     WRIEPGADRPFHPGRGGSVLVGDGRIGVVGELHPADADRLDLAGRVAVAELDVAALEV
                     ERARAVAFRDVPRFPPLRRDLAFIVPDEATAGDAQRILEAAAGSLLDRVVLFDLFTGG
                     DVPAGHRSLAFALEFRAPDRTLTDDEVEPVVAAIVDRVRSELGAELRA"
     gene            6145..7062
                     /gene="argF"
                     /locus_tag="VF195_12400"
     CDS             6145..7062
                     /gene="argF"
                     /locus_tag="VF195_12400"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019046475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEX6581657.1"
                     /translation="MRHYLSVDDLSTDELPDLLDLAAALKADRGAHDQALAGRSIALV
                     FEKPSTRTRVSFEVGVEQLGAHAVVLSSAELQLGRGETIQDTGRVLSRYVDAIVLRTF
                     EQERLEILSTTATVPVINALSDFEHPCQALADLLTIREHLGDLHGRVLTYLGDGNNVA
                     HSLLLAGTKAGMAVRVATPPGFEPIPQVVHRSEEIAAETGGSVELTHDPEGAAIGAHI
                     LSTDVWTSMGQDLEADERALVFPSYQLGQKLVDLADPDVIVLHCLPAHRGQEITDEVI
                     DGPRSVVWDQAENRLHTQKALLLRLFGVA"
BASE COUNT         1119 a         2406 c         2519 g         1075 t
ORIGIN      
        1 gagccacgct tgaacgacag gatccgatct ccccaggtgc gcctcgtcgg cgccgacggc
       61 gagcagatcg gggtggtcgc gacccaggaa gccgtccgca gggcacgcga gctcgacctg
      121 gacctcgtcg aggttgcacc gcaggcgaac ccgccggtct gtcggatcat ggactacggc
      181 aagttcaagt acgagcgaga catccgtcag aaggaagctc ggaagaagca ggcccgcacc
      241 gagctcaagg agatcaagtt ccgccccaag atcgacgcgc acgactacgg cacgaagaag
      301 ggccacgtcg aacggttcct ccggggcggg cacaaggtga aggtgaccat catgttccgg
      361 ggtcgcgaga tgacccacac ggagctcggg cgcaagatcc tggaccggtt ggtgggagac
      421 ctgggtgaga tggtgaccgt ggagtcgatg ccgaagcagg aggggcggaa catgatcatg
      481 gtgatctcgc ccaacaagcg gtacatggag cagctcgccc gcgcccgggc gcgcgaatca
      541 gcacccgtta tcgatgccgg ggcggtcgcc accgcagccg agacggacga gggatagccg
      601 atgccgaaga acaagagcca ccgagccacc gcgaagcggt tccggatcac ccggaacggc
      661 aaggtgctgc accgcaaggc caccggcaat cacatgctca cgaagaagtc gtcgtcccgc
      721 cgccgccgcg tcgagggcat ggccgaggtc gggtcgaaag ccgacgccaa gaccgtcaag
      781 cggctgctgg gggactgacc gatggcgcgt atcaagcgtt cggtgcacgc gaagaagaag
      841 cgccgagaga tcctcgaggc tgccaagggc tacacgggga gccgccgcaa gcgactccgg
      901 gtggccaagg aacaggtcat gcactccggc atgtacgcct accgcgaccg ccgcgaccgc
      961 aaggcacagt tccgccgcct ctggatcacg cgcatcaacg cggcggttcg cccccacggg
     1021 ctctcgtact cccgcttcgt gagcgggctg cacgccgccg gcgtcgaggt cgaccgcaag
     1081 atcctcgccg acctggcggt ccgtgacccg gaggccttcg cgcagctcgt cgcgaccgcg
     1141 aaggcgtcgg gtccctccga cttctagcgg gccgttgggg cccgcataca ctccgcccgt
     1201 gcgaggggtg cgcgagtcgc cgtgttgacg tccggcagga acccgaaggt cgtcgccgcc
     1261 gtccggttga agaagcgcgc cctccgcgag gcggatcgtc gcttcctcgt cgagggggcg
     1321 caggcggtgg cggaggcact tgccgaagac ggacgcctcg agtcgctgtt cgtggaggac
     1381 gatctcgacc cgctcgcgat ccgggcgaag caggccggcg tgaccgtcga ccacgtcacg
     1441 gagcgggtca tggaacggct gacgtccacc atcaccccac agggtgtcgt ggggatcgca
     1501 ccgttcgtcg acgtgaccct cgacgcgctg ggcccaccgg gcgccgtggc tctcttgcac
     1561 gaggtccggg atcccggcaa cgcgggcacg atcctccgct cggccgacgc tgcgggggcg
     1621 gtcggcgtcg tcttcgccgg cagctcggtg gacccgtaca acgccaagag cgtccgtgcg
     1681 tcggctggtt cgatcttcca cgtcccggtg gtgcgggacg tcgcgacggg ggacgcgatc
     1741 gcgaccctcc gggaccgagg gttcacgatc gtcgccatgg atatgcacgg ggacgaggac
     1801 ctgttcgaag cgcagctccc gccctcgtcc gcgttcgtct tcgggaacga ggcgcacggc
     1861 ctccccgccg agatcctcgg gacggccgag cgacgcgtgc gcgtgcccca ggcgggccgc
     1921 gcggagagcc tgaacctcgc agccgccgcc acgatctgcc tgttcgaatg ggtccggcga
     1981 agccgtgcga ccaccggaac cctcgaggcc ctggtcgcgg ccgccgcgca cgacatccgg
     2041 tcgccgctga cggccatgaa ggggttcggc tacgcactca gcaagcggtg gacggacatg
     2101 acggacgagc agcgcgccct gatgctcacc ggcatcgtcc acgacgccga ccgcatggac
     2161 acgatcgtcc ggcagctcct cgacgccgcc agggtgatga gcgggacctt ccagtcgttc
     2221 gccgagctcg tggacgtgtc cgacgtggtg cgtcagatca ccgagcagct ggcccgtgac
     2281 ccggaacacc cccagatcga gtggtcggga ggagccggag ccaaggcgat gctcgacggc
     2341 ggccggctga agacgacgct cctcgcgttc acggaggcgc tggtgtggtg ggcgagcgac
     2401 gggccgatcc ggatcgagag cgagctgtgg ggcgggcgcg tggtcgtgtc cgcctcacgg
     2461 gaggcccacg gcgacgtgga tgtcgcctca ctgttcgaag cccggcgacc gggggcgggc
     2521 cgcggctcca agatcggttt gtacgttgcg cgacgcgtgg ccgaggccca gggaggaagt
     2581 gtctcgggtg acctcgccga tggacggctg acgctccacg tcgagctccc cgccaccggt
     2641 cccgaacctc tggcgccgta gactcgcgga cctcatggac gtccagcaca tgcgcgaatc
     2701 ggtcgaggaa gtgcgagagc gcggtcgccg tgtgttcgcg gaggcatcgt cgcgcgaaga
     2761 gctggaggct gcgcaggtca cggtcctcgg gcggaagagc cttcttgccg aggtgcagcg
     2821 gtccctcgga gccctcgagc cggaggagcg caaggatctg ggtcggatcg tgaacgaggt
     2881 ccgagcggtc ttggagagcg cagcctcgga acgacgcgag gagctcgagt ccacggccga
     2941 ggcagcgttg gtcacggcgg accgcctcga tctcacgctt cccggacgcc gtccgagaac
     3001 ggggtcgatg catcccttca cgttgatcga gcacgagatc gtcgacatct tcacgaggat
     3061 gggctaccgg gtcatcgagg gctctgaggt cgaggacgag tggcacaact tcgatgctct
     3121 caacatccct ccggaccatc cggcgaggac ctcgatggac accacgttcg tcgacgtgcc
     3181 ggggcacccc gagctcgtct tgcgaaccca cacctcacct atgcagatcc gcacgctcga
     3241 gtcgcagcgg ccgccggtcg ccgtcgtggt gccgggtcgc gtgtatcgca acgaggagat
     3301 cacggcgagg aacatgccgg tgttccacca ggtggaaggc ctcctcgtcg acgaggggat
     3361 cacgttcgca gacttgaagg ggacgcttga ggtcttctac cgggcgctcc tgggtgagga
     3421 acgggagatc cggctcacgc cctccttctt cccgttcgtt gagcccggtg cccaggtcga
     3481 tgtctcgtgc ttcgtatgcg acggcgcggg ctgcaagacg tgctcgtact ccggctggct
     3541 cgagctcatg ggctcgggga tggtgcatcc ggtcgtgctc gagaactgcg gcctcgatcc
     3601 ggagcggtac acagggttcg cgttcggtgg gggctatgac cgtctggcga tgatccggtt
     3661 ccagatcccc gacatccgac tcctgtggga tccggatgtc cggttcctcg agcagttcga
     3721 ggccgccggc tgatgcgggt ccccgtcgga tggctgcgcg agcgatgtcc caccgacctg
     3781 tcgccggagg agctcagcga tctcctcgcg ctgaagggcg tccacctgga atcgatcgaa
     3841 cgtccgtggg agggactcgc cggcgtcgtc gtcgcccgcg tgctcgaggt ccgcgaccat
     3901 ccgaacgccg agaagctgtg catcgcgatc gtcgacggcg gcgacgggcc gacacaggtc
     3961 gtcgtgggga tccggaacat gaagcccggg gatctggtcc cgtgggcgcg accgggggcc
     4021 cgcgtgcccg tgctcgaccg tccgctggag gccaagccgc tccggggaga gatctccaac
     4081 gggatgttgt gctcgccccg ggagctcgcg gtctcccacg agcacgagac cggcatcatg
     4141 ctcctgcccg aagacctgac ggttggcgcg gacctcaggt cgacgctcgg gctcgacgac
     4201 gtcgtgctgg atctggagat cgaatccaac cggccggacc tcctgtcgat caccggcatc
     4261 gctcgggagg tggcgggggc caccggagag ccactcatcc gccccgacat cacggtcgcg
     4321 gaggtggggg agccggcctc gacggtggcg accgtcgaga tcagggatct gcagggatgc
     4381 ccgcgctacc tcgcccggat cgtgcgaggg atcgggccgg gacggacgcc gatcctcgtc
     4441 caggcgcggc tcacggcctg cggcgtgcgg ccgatctctc cggtcgtcga cgccacgaac
     4501 catgtgatgt tggacctcgg tcagccgctc cacgcgttcg acctgcagcg gctcgccggt
     4561 cccggcatcg tcgtgcggcg atccgagccg ggcgaacgcc tcgaaacgct cgacggcgtc
     4621 ggccgggagc tcgacgacga tctgctgatc tgcgacctcg aacgtccggt cgcgatcgcg
     4681 ggcgtgatgg gtggtgcatc gtcggaggtt gatcccggaa cggtcgacgt gctcctcgag
     4741 agcgcgtact tcgagccgcg atcgatcctg cgcacgtcca ggcgcctcca gctcctgacg
     4801 gaggcgtcgg tccgcttctc acgggggacg gaccccgaag gcgttggtcc cgcagcggcg
     4861 cgagccgccc ggttgatcgc ggcatggaca gggggcgcgg tgctggagga cgcggtcgac
     4921 gtcggaggac cgccgccgcg gcgttcgatc ggactccgtt cgagtcgcgc cgcggccctc
     4981 ctcggatacc ccgtcagcgc ggcagcggcg gcgcaggcgc tcgcgaagat cgagatcgct
     5041 gcggaaccga ccgacgatcg tgtcggggtg gaggtgccga gcttccgacc cgacctcgag
     5101 cgggaggagg acctgatcga agaggtcgta cggatccagg gcttcgaccg gctgccgtcc
     5161 accgtgccgg cgctccgaga ggtcggaagc gagcaggcga gctaccgcac caggcgccgg
     5221 gtccgggagc tgctcgtccg tgccggggtc cgtgaggccg cctcgctgac gttcgcgtcc
     5281 gctgccgagg tcgaactgat gcggcacacg gatcccgtgc gggtcgcgaa ccctccctcc
     5341 gcggaggaac cgttcctccg gaccagtctg ctcccgcgcc tcctcgaggc cgcgagtcgc
     5401 aatcagcaac gcggggagat ctcgatcgca ctgttcgaga tcgggcacgt cttccgcctc
     5461 gccgaccctg tcgacgagcg agagcacctc gggatcctcc tcgcgggccg ggcgggggag
     5521 gggctccacg ccgaggaccg cggatgggac gcgctggatg cgaaggggat cctcgatgtt
     5581 gtcctgggcg ggctcggcgt cggatggagg atcgagcccg gcgcggatcg accgttccat
     5641 ccgggtcggg gaggctcggt gctcgtcgga gacggtcgga taggcgtggt gggtgagctc
     5701 cacccggccg atgcggatcg tctcgacctc gccgggcgcg tcgccgtcgc cgagctcgat
     5761 gtcgcggcgc tcgaggtgga acgtgcacgg gccgtcgcgt tccgcgacgt tccgcggttc
     5821 ccgcccctcc gtcgcgacct cgccttcatc gtcccggacg aagccaccgc cggcgacgct
     5881 cagcggatcc tggaagctgc cgccgggtcg ctcctcgacc gggtcgtgct gttcgatctc
     5941 ttcaccggcg gtgacgtgcc cgccggtcac cggagcctcg cgttcgcgct ggagttccga
     6001 gccccggacc gaaccctgac ggacgacgag gtcgaaccgg tcgtggccgc gatcgtggac
     6061 cgagtccggt cggagctcgg ggccgagctc cgggcctgaa ggtcgcaaga ccctgtgtcg
     6121 acggtcccat tagactccgg cgtcatgcgt cattacctca gcgtcgacga cctctccacg
     6181 gacgagctcc ccgatctcct cgatctcgcc gcggcactga aggcggaccg gggcgcgcac
     6241 gaccaagcgc tggccggccg atcgatcgcg ctggtcttcg agaagccctc cacccgcacg
     6301 cgggtttcgt tcgaggtcgg ggtcgagcag ctcggcgcgc atgccgtggt gctgtcctcg
     6361 gcggagctcc agctcggtcg cggagagacg atccaggaca ccggtcgcgt cctgtcacgc
     6421 tacgtcgacg cgatcgtgct ccgaaccttc gagcaggagc ggctggagat cctctccacc
     6481 accgccacgg tcccggtgat caacgccctc tcggacttcg agcacccctg ccaggcgctg
     6541 gcggacctcc tcacgatccg cgagcatctc ggcgacctcc acgggcgtgt cctgacctac
     6601 ctgggggacg ggaacaatgt tgcgcattcc ttgctcctgg ccgggaccaa ggccggcatg
     6661 gccgtccgcg tcgccacacc gccgggcttc gagcccatcc cccaggtggt gcacaggtcc
     6721 gaggagatcg ctgccgagac aggtggctca gtcgagctca cacacgatcc cgagggcgcg
     6781 gcgatcggtg cgcacatcct gtccaccgat gtttggacga gtatgggtca ggacctcgag
     6841 gcggatgagc gcgccctcgt cttccccagc taccagctgg gccagaagct cgtggacctc
     6901 gcggaccccg atgtcatcgt cctccactgc ctgcccgcgc accgcgggca ggagatcacc
     6961 gacgaggtga tcgacggacc gcggtccgtg gtctgggacc aggccgagaa ccggctccac
     7021 acgcagaagg cgctgctgct ccgcctcttc ggcgtagcct gaggcgatgc cccgggcgaa
     7081 cgacgccatc gaggcgatgc tcatcgagta cgccgacct
//
LOCUS       DASUYD010000235         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_402672, whole genome shotgun sequence.
ACCESSION   DASUYD010000235 DASUYD010000000
VERSION     DASUYD010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402672"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..207)
                     /locus_tag="VF195_12405"
     CDS             complement(40..207)
                     /locus_tag="VF195_12405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581658.1"
                     /translation="MVEVPVDEEPERPAFAFFAWRFSFSDLLAAVFELFEPPLSLLAM
                     VASSRRERSER"
     gene            446..751
                     /locus_tag="VF195_12410"
     CDS             446..751
                     /locus_tag="VF195_12410"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEX6581659.1"
                     /translation="MKHVRIATYDIKKGSFQELANVAQDGMLREFRNQPGFIRYGLAD
                     SGDKTCVSLSLWETRKDADHAVDVSAAWDRAHIADRAVLRTSQVGDLAFYEGVPAKV"
     gene            941..1312
                     /locus_tag="VF195_12415"
     CDS             941..1312
                     /locus_tag="VF195_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015324936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX6581660.1"
                     /translation="MQKPKSVEAYLESIPEERRSALQKLRETIVAAAPEAEEGITYSM
                     PGFLLDGRGFVAYAAFDDHYSFFPMSSKVIDAHRDELGSRVTGKGTIRLEYGRPLPTA
                     LVKKIVRTRLAEATAKRRGRK"
     gene            complement(1345..1896)
                     /locus_tag="VF195_12420"
     CDS             complement(1345..1896)
                     /locus_tag="VF195_12420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019511836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEX6581661.1"
                     /translation="MPTSVALLRGINVGGKNLIGMPALKACFEENGFDDVVTYIQSGN
                     VLFTAPTTPAAELTRRIEAMLALAFDYVPTVVVRTRRQMRAIVDRAPKGFGREPAKYR
                     YDLIFLKEPLAAKVALQSVPTKPGVDAAHPGIGVLYFSRLSAKASQSRMSRIVSSPIY
                     ASVTIRNWNTTTRLLSMMDERHT"
     gene            complement(2060..>2444)
                     /locus_tag="VF195_12425"
     CDS             complement(2060..>2444)
                     /locus_tag="VF195_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX6581662.1"
                     /translation="APDSPSEQAIVAEFVRAYESGDLDDLVALLTDDVFLSMPPLPLE
                     YEGRDVVARFYASMIGSGRRFDLVPTRANGQPAFGAYLRASTGIRHGTGLLVLTLTGD
                     RICSVTRFDNSVLPWFGLPRSLPSR"
BASE COUNT          457 a          761 c          836 g          390 t
ORIGIN      
        1 gtgttgttcc ctccctcgcc gggtcccaga gtggtacgtt cagcgctcgg atctctccct
       61 tctcgaggag gcaaccatgg ccaacaagga caagggcggt tcgaagagct cgaagaccgc
      121 ggcgagcaag tcgctgaagg aaaagcgcca agcgaagaag gcgaaagccg gtcgttctgg
      181 ctcctcgtcg acggggacct cgaccaccag gtagccctcg ggatacaccg gaccggtcgt
      241 gggtgtgcgg caaagacgta cactcaacgc agacgcgagc cccgtacctg ttagggagaa
      301 gaggactcac ggggatcgtc tccacgatcc ggtcccatcg ctggtcccag cgaaggcttg
      361 atatcccgaa gcgcgcgcac cacttcggat ctggctcacg ttggccccgg ttggtcaggg
      421 ccacgacgag caaaggagac cacgcatgaa gcacgttcgt atcgcaacgt atgacatcaa
      481 gaagggttcc ttccaggagc tcgcaaacgt cgcacaggac gggatgcttc gcgagttccg
      541 gaaccagccc gggttcatcc ggtacggctt ggccgacagc ggcgacaaga cctgcgtctc
      601 gctcagcctg tgggagacac gcaaggacgc cgatcatgcg gtcgacgtgt ccgctgcctg
      661 ggaccgtgcc catatcgccg acagggccgt tttgcgcaca agccaggttg gcgacctcgc
      721 cttctacgaa ggcgttccgg caaaggtctg atcgacttcg gtcgtggacc ggcccgcccg
      781 tgcgacccac gagcgcggct gtgaggagga aggggcccac ctcggtgggt cccttctttc
      841 gttgtgacgc cgccggagag agcgtcatgc cgccgagcgc cgatcgggcc caacgccgcc
      901 gcggccatga tggttcgaga cacatcgcga aggagtgacc atgcagaagc cgaagagcgt
      961 cgaggcatac ctcgagtcca tcccggagga acgacgatcg gcgctccaga agctgcggga
     1021 gacgatcgtc gccgcggcgc ctgaggccga ggaggggatc acctacagca tgccggggtt
     1081 cctgctcgac ggccgcgggt tcgtcgccta cgctgcgttc gacgaccact acagcttctt
     1141 cccgatgagc tcgaaggtga tcgacgcgca tcgggacgag ctcgggtcgc gtgtcaccgg
     1201 gaaggggacg atccggttgg agtacggccg gccgctgccg acggcgctcg tgaagaagat
     1261 cgtccggacg cgcctcgccg aggcgaccgc caagcggcgc gggcggaaat gaccgaggcc
     1321 ggacggctcc agaccctggc agcgtcaggt gtgccgctcg tccatcatcg acagcagcct
     1381 cgtcgtcgta ttccagttcc tgatcgtcac gctcgcgtag atcggcgacg agacgatcct
     1441 gctcatgcgg ctctgtgagg ccttcgcgct gagcctcgag aagtagagga cgccgatacc
     1501 gggatgcgct gcgtcgacgc ccggcttcgt cggcacgctt tgtaaggcga cctttgcagc
     1561 gagaggctcc ttcaagaaga tgaggtcgta gcggtacttc gcgggctctc gaccgaaccc
     1621 cttcggcgcg cggtcgacga tggcgcgcat ctgtcttcgg gtccgcacca ccacggtcgg
     1681 cacgtagtcg aacgcaaggg cgagcatcgc ctcgatgcgc cgcgtcagct ccgcagcggg
     1741 ggtcgtggga gcggtgaaca gcacgttgcc actctggata taggtgacca cgtcgtcgaa
     1801 cccgttctcc tcgaaacacg ccttgagcgc gggcatcccg atcaggttct tgccgccgac
     1861 gttgatcccg cggaggaggg cgacggaggt cggcaaacct acgcgggctt cctcgcgggg
     1921 aggccgtagt gccaggtccg gggctccgta ggcgggtccg aggacatcgt cgaccgatcg
     1981 taacggtgcg atcgaacgtc aggccatctc gaaacgcagc cgactaggtc gaaagaggcc
     2041 ctctcgcact cggaagctgc tatcggctgg gaagcgatcg cggcagcccg aaccatggga
     2101 gcacgctgtt gtcgaagcgg gtgacggagc agatccggtc gcccgtgagg gtgaggacga
     2161 gcaggccggt accgtggcgg atgccggtcg aggcgcggag gtaggccccg aacgccggct
     2221 gaccgttggc tcgcgtcggt acgaggtcga acctccggcc cgagccgatc atgcttgcgt
     2281 agaagcgagc cacgacgtca cgaccttcgt attcgagggg caacggtggc atcgagagga
     2341 agacatcgtc ggtgagcagg gcgaccaggt catcgagatc ccctgattcg taggcgcgga
     2401 cgaactccgc aacgatcgcc tgctcggagg gtgagtcggg agcg
//
LOCUS       DASUYD010000236        16922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_404520, whole genome shotgun sequence.
ACCESSION   DASUYD010000236 DASUYD010000000
VERSION     DASUYD010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 16922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16922
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404520"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..410)
                     /locus_tag="VF195_12430"
     CDS             complement(177..410)
                     /locus_tag="VF195_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581663.1"
                     /translation="MASRKAMQAEGDVIRFPERAAVPVDFSTFFAEVDDTIRVWIVDV
                     GGTRLFLEAITTEDATDGLFREVKQIIESIRFG"
     gene            complement(505..1281)
                     /locus_tag="VF195_12435"
     CDS             complement(505..1281)
                     /locus_tag="VF195_12435"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HEX6581664.1"
                     /translation="MHDEVLEHFLDEGLIEQVIRPIKSGKEASLHLVRANPRTTGEEL
                     AALKIYHPLDRRDFRDESLYRDGEFIKERRIRVALEKKTRFGREVQGGIWVGREWETL
                     HTLFAAGAPVPRPIEATDHAILMSYIGDEDDAAPQLHRYRATDDNEVEDLLEQCIRVV
                     ERMLFHDVVHGDLSPFNILVWDGRISVIDLPQAVDPKKNRHAEALLARDVERVCAHFD
                     RLGVSCDAGRVTEDLWTAWTFADLVPEELRATLEVGPPAG"
     gene            complement(1513..1896)
                     /locus_tag="VF195_12440"
     CDS             complement(1513..1896)
                     /locus_tag="VF195_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581665.1"
                     /translation="MAVVPIGGGDAGVGQDPDGLALGPSERLAGSVENPTDPPEFPLV
                     LVTWRDAWFDFEEPDPEDVRADYLVNTVGYLVRRGPRFVSVAQEILPDGDGFRAVTHI
                     PVAVVETIASLRDSDAAGMDDVLAL"
     gene            2080..3423
                     /locus_tag="VF195_12445"
     CDS             2080..3423
                     /locus_tag="VF195_12445"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX6581666.1"
                     /translation="MPRPRTFFACSECRQQVAQWVGRCPGCGTWGTIESALGATAVAG
                     GPVLTLGGGTVAVEPRVSTGIPGLDRVLGGGLVPASVILLAGEPGIGKSTLLLQLLSS
                     LAGDGRGCLLVSGEESHGQVAARSRRLGVAGDAVAFAPGRDLRAVLATARERDPFLLA
                     VDSIQSVRDPSAPQMAGGVAQVRNCTDALIGMAKEEGIAVLMTGHVTKDGDLAGPRAL
                     EHAVDVVLLFEGDARSGSRVLSGGKNRFGAEGESAWFEMAPDGLHEIDPTGLLLEGVG
                     IPGAAIALPMAGRRALAVEVQALMGSVDGPARRQTTGLDARRFQLMAAVLARAAGYRL
                     GRAELFGATSGGLRVEDPAADLAVAAALASAVTGTPTPEGAAFVGEVSLTGLVRRVSG
                     MPQRLAAAEAAGCSRVFGAPGPETQRHPLLTPVRHVSEALSWTHDSAAATRRRAS"
     gene            3578..4093
                     /locus_tag="VF195_12450"
     CDS             3578..4093
                     /locus_tag="VF195_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006545253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX6581667.1"
                     /translation="MSAFRKGDTVVHPEHGAAVIEELKDKEFLGEKRKYLVLRVVYGD
                     LTLSVPVDSAEQVGLRPVISKPQVKKILKVLTEAETISAANWSRRFKNNLEKLHSGDP
                     FEVAEVLRNLSIRDREKGLSAGEKRMIHKARQILISELSHAAGKSEEDAELMIDEVLD
                     GAHGVKMAAEA"
     gene            4203..5294
                     /locus_tag="VF195_12455"
     CDS             4203..5294
                     /locus_tag="VF195_12455"
                     /inference="COORDINATES: protein motif:HMM:NF014047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEX6581668.1"
                     /translation="MTENEHGRGDVLRGRVAGGVRMIFVALSATVGARLGEASRTTST
                     SRALLFVFLGAGVGYVIGGVFGRLTLRAVHGLEAELGRKPPAVLAGGVVGLIVGLLVA
                     GLMMIPLLFLPASAAWPAIVFLYLAFGTLGYRLGQSKYEDIFSLVGVKPRAAGVTRGD
                     LHVLDTSALIDGRVADLVATGFVAGTVLLHEGVLRELQAISDSSDPHRRTRGRRGLDM
                     LVELQKAPTLQFQLVEELGVMDVDAALVRLARERGAQLITVDHNLAKVAEALRVPVAQ
                     INALASRFRVPYTAGDELAVRVVKEGREHGQAVAYLDDGTMVVVEQAATRIGSQVEVR
                     VTNVIQTTTGRMIFAALSGDSPATAGADA"
     gene            5321..6010
                     /gene="ispD"
                     /locus_tag="VF195_12460"
     CDS             5321..6010
                     /gene="ispD"
                     /locus_tag="VF195_12460"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEX6581669.1"
                     /translation="MEPDAAIVILLAAGAGRRLGGPEPKVLLPIGGRPVLAVAAAAAA
                     ASGVDGLVVTFPPGWEGRARACVVGLEVDVRFVEGGETRQASVRAALEAVPASVAIVA
                     VHDAARPFASPDLFRRVLRAVERGPDGAVPVLPVADTVLRVRREVVEGVESREELALG
                     QTPQAFRTAALREAHSHAEAVGASFTDDASMLRWAGYEVLAVPGDPDNVKITTLADLA
                     HADRRMGAAGG"
     gene            6003..6473
                     /gene="ispF"
                     /locus_tag="VF195_12465"
     CDS             6003..6473
                     /gene="ispF"
                     /locus_tag="VF195_12465"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009215000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEX6581670.1"
                     /translation="MADQAVGLGFDVHAFAPGRPLWLGGLRFEGQPGLAGHSDGDVVC
                     HAIADALLGAVALGDVGAHFPDADPATAGISGEELLSRTLGLLAEGGRSPVSVDVTIV
                     CDRPSIATRRLEMRERLARIAGLPVERVSVKATRPEGLGLTGEGVGCLALAVVA"
     gene            6470..7234
                     /gene="rlmB"
                     /locus_tag="VF195_12470"
     CDS             6470..7234
                     /gene="rlmB"
                     /locus_tag="VF195_12470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEX6581671.1"
                     /translation="MSGEVVTGRRAVVEALRAGQVREVLVAAGARDTQGLRAVREAAR
                     AARVPVRATDRRALDALAGDHRGVVARTVGGRAGPPQLGERDLATLAFAPDAIIVVLD
                     GIMDPQNLGAAARTAEAAGAAALVSRSRRAAGVTPAAIRASAGALEHLPHAVVANIAR
                     ALARLQDAGFSVIGLDDGASSSIFDEECPTGRVALVVGSEGEGMSRLVRERCDLLVAL
                     PVKGRVGSLNASAALAAALYGFVLPREVVASPSSPG"
     gene            7265..7729
                     /locus_tag="VF195_12475"
     CDS             7265..7729
                     /locus_tag="VF195_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581672.1"
                     /translation="MNDIPPPPSPGTVPGVPTPRPTGPPTSRPGAVTGAAAILLIAGA
                     LVTIFGLMGLTGNGVDIDAPFLDGDAAARIVALALVVQGILSLLAGWLVLRLRPAGRV
                     LGLLLAALGIVGGLAQVRRTGSAGLLTFTLNAFVIYVLLTYGFLFKPREIPR"
     gene            7741..7814
                     /locus_tag="VF195_12480"
     tRNA            7741..7814
                     /locus_tag="VF195_12480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7775..7777,aa:Thr,seq:tgt)
     gene            complement(7854..10040)
                     /locus_tag="VF195_12485"
     CDS             complement(7854..10040)
                     /locus_tag="VF195_12485"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581673.1"
                     /translation="MARSEEIGENAAPSADLRFVAQLNMLHSMAATLNSLDDVSEIAA
                     AITDELHTILDYHNCRVYVMQDDGSTLLPVAFRGEIFYEYEHETLEELITHLGEGITG
                     WVAEHRTSLLTADARLVEFAVQIPDTDDILESMLAVPMTVGDAVEGVIVLSSLGYGAF
                     DEEDQRLLEVLASHAAIAISNAKLLQAERTAAETSSALLGLSEQLTQLRSVGDILQGA
                     IETVSSLIGEAAVAFHIRDEHTGDFRLARLLTLDPDAIRPRHEIGDVPESVALSVVFD
                     NEEPLVLPRTSPLMHGDYLVSKEPRELLVAPMTWEPDGAGTISVVGRHGDGLFGDREL
                     RLIRGVADLTSLALGNARRMSELERFHELVAGLDAAFWEASIPGMEFTFVAGAVEDLF
                     GGGAGSWPDQDRTWGSHIDARDREEATRALLRAIESGVGEGLEYRVPSDQGSPRWIRD
                     LVHVIRGRGGAPTILRGLMVDITERKRAERALAASERKYSDAFKREREAAQRLRMLDD
                     MKNTFLEAVSHDLRTPLTAILGSALTLERSKLDLPTDDALDMVGRIAANAKKLERLLS
                     DLLDLDRLQRGIVSPKRRATDLEGFVKRAVKEMDTRGRRIELDVQRGSVQIDQAKTER
                     ILENLLSNSIRHTPEEAKIWVRARPHDGGVLFVVEDDGPGVPSELREAVFEPFRQAPG
                     SSSVHAPGVGIGLSLVRRFAELHGGNAWAEEREGGGASFHVFLPGS"
     gene            10138..10458
                     /locus_tag="VF195_12490"
     CDS             10138..10458
                     /locus_tag="VF195_12490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HEX6581674.1"
                     /translation="MGDRRRSARPGSEVASDPASEPSVALDERSREVLDFEREAWKLT
                     VPKQRAIRERFGFSPSRYHQLLHRIIDRPEAVAYDPMLVRRLRRVRAVRRRFRTAQGL
                     GVRV"
     gene            10523..11032
                     /locus_tag="VF195_12495"
     CDS             10523..11032
                     /locus_tag="VF195_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEX6581675.1"
                     /translation="MNPGIARLAIVIALVIGGVAVLANGFDDEGAAAPPEPSTSQSPT
                     GTPSPSPSPQASVVPNQKGVLVQVLNGTSTAGYAADFQEMLVDLGEYLPAGDAGDAPD
                     KPIVDSIVYFRADDNRAQNEADAQLLSDEYLGGVPVQRLPADLARSGAIDAAADVVVV
                     LGEDQAGEL"
     gene            complement(11037..11414)
                     /locus_tag="VF195_12500"
     CDS             complement(11037..11414)
                     /locus_tag="VF195_12500"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX6581676.1"
                     /translation="MGRVLVVDDEPDVLLLCRLNLQQRGHELLEAADGSTALAIARDR
                     HPDVIVLDLMLPGISGYDVLEALQRDAETTNIPVLVLTAKSLRADRERSHGLGASAFL
                     TKPFLPNELCEMVDSLVAPRHGA"
     gene            11605..12075
                     /locus_tag="VF195_12505"
     CDS             11605..12075
                     /locus_tag="VF195_12505"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEX6581677.1"
                     /translation="MLKVSQKLEYAMRAMIELADATHAGVLVPAREIAARQQIPLRFL
                     EQQLGALSKAGLVESFRGGGGGCRLARSPDEVTVAEIADAIEGQIFPMFCLEPSDHTC
                     FQDSQCGLQGFWADVARAVQAVFAETTVADLAARHRSMSPGLVLVSPEQLLSRP"
     gene            complement(12072..12236)
                     /locus_tag="VF195_12510"
     CDS             complement(12072..12236)
                     /locus_tag="VF195_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018351458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEX6581678.1"
                     /translation="MAQYEAYCVKCKAKREFEGTVVTLKNGRNAASGTCPVCGTKVMR
                     ILGKADVAAS"
     gene            12420..14381
                     /gene="acs"
                     /locus_tag="VF195_12515"
     CDS             12420..14381
                     /gene="acs"
                     /locus_tag="VF195_12515"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX6581679.1"
                     /translation="MTDAQPHIDALLQEERVFPPPSRFAEGAVVSDPAIYERAAEDPE
                     AFWSEQAGRLEWSRPWDTVMEWAPPFVKWFVGGTLNASVNCLDRHVASGGGEKVAYHW
                     EGEPGDTRTLTYRQLLDEVCRLANGLRSLGVRTGDRVNIYLGMVPELPIAMLACARIG
                     APHSVVFGGFSADSLRDRINDAEAKVLITADGAWRRGQIVPLKANADEAVADCPSIEH
                     VITLRRCENEHAFTPGRDIWYHELIGEQSTECVPEEMDAEDVLYLLYTSGTTGKPKGI
                     THTTGGYLTQVAATHRMIFDIKDDDVFWCAADCGWVTGHSYIVYGPLANHTTGIMYEG
                     APDWPDKDRWWSIVETYGATILYTAPTAIRAFMRWGTDYPEKHDLSTLRLLGSVGEPI
                     NPEAWVWYWKVIGGERAPVVDTWWQTETGAILITPLPGLTELKPGSATIPFPGIRADV
                     VDEQGVTVPLGGGGYLVLTRPWPAIARTIWGDRDRYAETYFSRFGPEIYVAGDGAKRD
                     EDGYFWLLGRIDDVMNVAGHRLSTFEIESALVDDDKVAEAAVVSRPDDLVGEAIVAFV
                     TLKTGYEGDDAMAKELREHVAAKIGKIARPHGIIFTADLPKTRSGKIMRRLLRDVAHG
                     RTLGDVTTLANAEVVEQIREMSESAPADE"
     gene            complement(14420..16213)
                     /locus_tag="VF195_12520"
     CDS             complement(14420..16213)
                     /locus_tag="VF195_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX6581680.1"
                     /translation="MSARPGWTLRFTHPSIEQVVWASSAPDRAAAVTTEDGSTQAWAW
                     DVRANERRRVSTGGVGAEEAHILPDGSGVVWWYDEIGNERGRWMLTPFEGGEAQPLFR
                     GFHDEWMMGLSLIDGGMAAGFATDEDYHVFVAMGSEAPRQIYRNPEPAGVGSEWPQGG
                     GGLSPDGALVCIHHSENGDIDRQALRVLDARSGESAGDQVDQGLRMEAVAWSPAGGAR
                     RLLFTQERSGIERPAIWDLDTRERLDLDLSDVTGPVIPLGWTLDGSRILAHNDPGEGV
                     NRLFTIDPVAGSVDETVRREGTIYEAGFRSGGDLWYRGDSSVEPPSVQDAAGAAILEL
                     PVDPPPPGTRSRWLTWTNPSRDLITGFLTTPTGSGPFPTIASIHGGPEWHHTDLWDPG
                     VQAFVDEGFAVLQVNYRGSSGRGKAFREELKGNIGFPETEDIVAGVDHVVAEGFADPR
                     ALFLEGWSWGGYLTTLIAGLHPDRWRAACAGIPVGDYVAAHYESAPTLRAWDVATMGG
                     SPMDLPELYRERNPMTYVDRVQAPMLLIAGEHDSRCPLGQVMVYAHALRARGHEVDVH
                     LYGGGHHALQVEEKIAHARRSIDFFRRHLAE"
     gene            16426..>16922
                     /locus_tag="VF195_12525"
     CDS             16426..>16922
                     /locus_tag="VF195_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX6581681.1"
                     /translation="MAPKIISFDEDARRALERGMDQLANAVKITLGPKGRNVVLEKKW
                     GAPTITNDGVSIAKEIELEDPLEKVGAELVKEVAKKTDDVAGDGTTTATVLAQSIVKE
                     GLRNVAAGANPMSIKRGIEKAVELAVEAIKNSAKEVDSKDDIAHVGAISAADPEIGEK
                     IAEAV"
BASE COUNT         2519 a         5874 c         5921 g         2608 t
ORIGIN      
        1 atcacgttcg agcacggcgc cggcatcatg ccgcccgaca cgccgcctgt ggagctcgcg
       61 ggctcgaccg agtaggtcga acccgcgtca cgcgccgcgc gtcggagcgc ggcggcaagg
      121 tcgaagcccg gtccgtcgga gaccgggctc ctcggcacct cgggccagcg gaggcctcac
      181 ccgaaacgga tcgattcgat gatctgcttg acctcccgga agagtccatc ggtcgcgtcc
      241 tcggtggtga tggcctcgag gaagaggcgc gtcccgccca cgtcgacgat ccagaccctg
      301 atcgtgtcgt ccacctcggc gaagaacgtc gagaagtcga ccgggaccgc ggcccgctcc
      361 gggaaccgta tgacgtcgcc ctctgcctgc atcgccttcc tagatgccac cgggccatca
      421 cgcgcttagg cgatcaaggc attcgtacgc aggtccgctg cgtcggaagt gggaacgacg
      481 ttgacgttgg tcgctcctcc ggcatcaacc ggccggagga cccacctcca gagtggcccg
      541 gagttcctct gggaccaggt cggcgaacgt ccacgcggtc cacaggtcct ccgtcacccg
      601 cccggcgtcg cacgacacgc ccagccggtc gaagtgcgcg cagacgcgct cgacatcccg
      661 cgcgagcagc gcttccgcat gacggttctt cttcgggtcg acggcctgcg ggaggtcgat
      721 cacggagatc cgcccgtccc agacgaggat gttgaacggc gagaggtccc cgtggaccac
      781 gtcgtggaag agcatccgtt ccacgacgcg gatgcactgc tcgaggagat cctccacctc
      841 gttgtcgtcg gttgcccgat agcggtggag ctgcggcgcg gcgtcgtcct cgtcaccgat
      901 gtacgacatg aggatcgcat ggtcggtggc ctcgatcggc cgggggacgg gagcccccgc
      961 ggcgaacagc gtgtgcaggg tctcccactc gcggccgacc cagatccccc cctggacctc
     1021 acgaccgaac cgtgtcttct tctccagcgc gacgcggatc cgccgttcct tgatgaactc
     1081 gccatcgcgg tagagcgact cgtctcggaa gtcgcgacgg tcgagcgggt ggtagatctt
     1141 cagcgccgcc agttcttcgc cggtcgtgcg cgggttcgcg cgtacgaggt gcaggctcgc
     1201 ttccttgcca ctctttatgg gccggatgac ctgttcgatg agcccttcgt cgaggaagtg
     1261 ctcgaggact tcgtcatgca acgcgggtgc ccccttcgtc gggatgttgg cgatcgcagg
     1321 gttgcggaca tgggcgacgc cgcgtccttc ggaagctacc gcgtgggagc cgagcggctc
     1381 agcggagggc ggagggacgc tgcgtgctga cggtgaccgt gtgggcattc atctcgcatc
     1441 cctccttcgg tgtcaggtga ggtggcacat ggcaggtgcg ccgatcgcac cgagccggcc
     1501 gagtatacgc cttcacaggg cgaggacgtc gtccatcccc gccgcgtcgg agtcgcgcag
     1561 ggacgcgatg gtctcgacga ccgcgaccgg gatgtgcgtc accgcgcgga acccgtcgcc
     1621 gtcgggaagg atctcctggg ccaccgacac gaagcgcggg ccgcgccgca ccaggtatcc
     1681 gacggtgttg accaggtagt cggcccggac gtcctcgggg tccggctcct cgaagtcgaa
     1741 ccaggcgtca cgccaggtca cgaggacgag ggggaactcg ggcgggtcgg tggggttctc
     1801 gaccgacccg gcgagcctct cagacggccc gagagccagc ccgtccggat cttggcccac
     1861 tccggcatct ccgccgccga tcggtacgac cgccacgacc gtgcctcctt cgtcgtcaag
     1921 acatctatcg gcatgtcttg cgcaactcgt agctgccggg gtcagatctc acaccgccgg
     1981 ccgcggatcc ccgccgatgg tcctaatcag aggaaggtag ccgtcgccac cgacaccgct
     2041 ccgacggtgg tgtcagaccc ctcggctagc gtccgctcca tgccacggcc ccggaccttc
     2101 ttcgcgtgct ccgagtgccg gcaacaggtt gcccagtggg tcggccggtg ccccgggtgc
     2161 ggcacgtggg ggacgatcga gagcgccctc ggagccaccg cagtggctgg tgggcccgtc
     2221 ctcacgctgg gaggaggcac cgtagccgtc gaaccccgcg tgtccaccgg gatcccgggg
     2281 ctcgaccggg tgctcggtgg cgggctggtc cccgcctcgg tgatcctcct cgcgggggag
     2341 cccgggatcg ggaagtccac gctgctcctc cagctcctct cgagcctcgc tggggacggc
     2401 cgcggatgcc tgctcgtgtc cggcgaggag tctcacgggc aggtcgccgc ccggtcgcgc
     2461 cggctcggcg tggcggggga tgcggtcgcg ttcgcgccgg ggcgcgacct gcgcgccgtg
     2521 ctggcgaccg cccgagagcg cgacccgttc ctcctcgcgg tcgactcgat ccaatcggtc
     2581 cgcgatccgt ccgcacctca gatggcgggc ggcgtggccc aggtgcggaa ctgcacggac
     2641 gccctcatcg gcatggccaa ggaggagggg atcgcggtcc tcatgaccgg ccacgtcacc
     2701 aaggatggcg atctcgccgg acctcgggcg ctcgagcacg cggtcgacgt cgtgctcttg
     2761 ttcgagggcg acgcacggtc tggttcgcgc gtgctttcgg gcgggaagaa ccgcttcggc
     2821 gccgagggcg agtccgcgtg gttcgagatg gcaccggacg ggctccacga gatcgatccc
     2881 acgggtcttc tgctcgaggg cgtcgggatc cccggcgcgg ccatcgcgct ccccatggcg
     2941 ggcaggaggg ccctcgcggt cgaggttcag gcactgatgg ggtcggtcga cggacccgcc
     3001 cgccgtcaga cgacgggtct ggacgcccgg cgtttccagc tcatggcggc cgtgctcgct
     3061 cgggccgccg gctaccgcct cggccgggcg gagctgttcg gagccacgtc gggtgggctc
     3121 cgggtcgaag atccggccgc cgacctcgcc gtcgcggccg ccctcgcctc ggcggtgacg
     3181 ggtacaccca cgcccgaagg tgccgcgttc gtgggggagg tctcgctcac ggggctcgtg
     3241 cgacgggttt ccggcatgcc gcagcggctc gccgcggccg aggccgcagg ctgctcacgg
     3301 gtcttcgggg cgcccggtcc cgagacgcag cggcatccgc tcttgacccc cgtgcgtcac
     3361 gtgtccgagg cgctgtcgtg gacgcacgac agcgccgcgg caacgcggcg gcgagcatcc
     3421 tgaacggccg agcgatcgct ccgtgactcg ccgaacatgc aggaaagtgg cctctgacct
     3481 gcggttttgg gttgcggagg aggacgttcc gtgttaccct tctatcctcg cccggccagg
     3541 ctccggccgg acgcacctct cgcgaaggga gatccacttg tccgcgttcc gcaaaggaga
     3601 caccgtcgtc caccccgagc acggggcggc agtcatcgaa gagctgaagg acaaggagtt
     3661 cctgggcgag aagcggaaat acctggtcct tcgggtcgtg tacggggacc tgacgctgtc
     3721 cgtcccggtc gactcggccg agcaggtcgg gctccggccc gtcatcagca agcctcaggt
     3781 caagaagatc ctcaaggtcc tcacggaggc cgagaccatc tccgccgcca actggagcag
     3841 gcgcttcaag aacaacctcg agaagctgca ctccggtgac ccgttcgagg tcgccgaggt
     3901 cctcaggaac ctctcgatca gggatcggga gaaggggctt tccgccgggg agaagcgcat
     3961 gatccacaag gcacggcaga tcctgatctc ggagctgtcg catgcggcgg gcaagtccga
     4021 ggaggacgcc gagctcatga tcgacgaggt cctcgacgga gcccacggcg tgaagatggc
     4081 cgccgaggcc tgacccgaac ctcacccgtt ccgttccggc tcaggagcgg ccccggatgc
     4141 gccgatgacc ctgtcagggg ccggatggtc tcgatcgagc ttcccgaggc ggggtgagcg
     4201 tcgtgacgga aaacgagcac gggcggggcg acgtcctccg cggccgcgtg gccgggggcg
     4261 tcaggatgat cttcgtcgcg ctgagcgcga ccgtcggtgc tcggctcggc gaggcctccc
     4321 gcaccaccag caccagtcgc gccctcctgt tcgtgttcct cggagcaggc gtgggctacg
     4381 tgatcggcgg cgtcttcgga cgcctcaccc tccgcgcggt ccacggcctc gaagccgagc
     4441 tcggcaggaa gcctcccgcg gtcctcgccg gtggcgtggt cggactgatc gtggggctgc
     4501 tcgtagccgg gctgatgatg atccccctcc tgttcctgcc ggcctccgcg gcgtggccag
     4561 ccatcgtgtt cctctacctc gccttcggga cgctcggcta ccggttgggg cagtccaagt
     4621 acgaggacat cttcagcctc gtcggtgtca agccgcgcgc cgccggcgtc acgaggggcg
     4681 acctgcacgt gctcgacacg agtgcgttga tcgacggacg cgtcgccgac ctcgtggcca
     4741 cgggattcgt ggcgggaacg gtgctgttgc acgaaggcgt gctgcgcgag ctccaagcga
     4801 tctccgactc ctcggaccct catcgtcgaa cgcgcggtcg ccgtggactc gacatgctcg
     4861 tggaactcca gaaggcgccc accctgcagt tccagctcgt cgaggagctg ggggtgatgg
     4921 acgtcgatgc cgcgctcgtc cggctggccc gggagcgcgg cgcccagctg atcaccgtcg
     4981 atcacaacct ggcgaaggtc gccgaggcgc tccgcgtgcc ggtagcgcag atcaacgcgc
     5041 tggcctcgcg cttccgggtt ccctacactg ccggcgacga gctcgccgtc cgcgtcgtca
     5101 aggaagggcg tgagcacggc caggccgtcg cctacctcga cgacggcacg atggtcgtcg
     5161 tcgagcaggc cgcgacgcgt atcggctcac aggtcgaggt cagggtgacc aacgtgatcc
     5221 agacgaccac cggacggatg atcttcgccg ctctctcggg tgattcgccc gcgacggccg
     5281 gcgccgacgc ctgaccgtcc cggtctgcga tgctgcgagc gtggagcccg atgcggcgat
     5341 cgtgatcctg ctggccgccg gcgccggtcg ccgactcggc ggcccggaac cgaaggtgct
     5401 cctcccgatc ggcggtcgac ccgtcctcgc ggtggccgcc gcagccgccg cggcttccgg
     5461 ggtggacggg ctcgtggtga cgttcccgcc gggctgggaa ggacgcgcgc gcgcctgcgt
     5521 ggtgggcctc gaggtcgacg tgcggttcgt cgaggggggc gagacgcgac aggcgtcggt
     5581 ccgggcggcc ctcgaagccg tgcccgcatc cgtggccatc gtggccgtcc acgacgccgc
     5641 ccgacccttc gcctccccgg acctgttccg gcgggtcctc cgcgccgtgg agcgaggtcc
     5701 cgacggcgcg gttcccgtcc tcccggtcgc cgatacggtg ctgcgggttc gcagggaggt
     5761 ggtggagggc gtcgaatcac gtgaggagct ggcgctcgga cagaccccgc aggcgttccg
     5821 gacggccgcc ctgcgggaag cgcactcgca cgcggaggcc gtcggcgctt cattcaccga
     5881 tgatgcgtcg atgctccggt gggcggggta cgaggtgctc gcggtaccgg gcgatccgga
     5941 caacgtgaag atcaccactc tggccgacct cgcgcacgcg gatcgacgga tgggcgccgc
     6001 cggtggctga tcaagcagtc ggtctgggct tcgacgtcca cgcgttcgcg cccggacgtc
     6061 ccctgtggct cgggggcctc cggttcgaag gccaacccgg tctcgccgga cactccgacg
     6121 gcgacgtggt ctgccacgcg atcgccgacg cgctgctcgg cgccgtggcg ctcggcgacg
     6181 tgggcgcgca cttcccggac gccgatccgg cgaccgcagg catctcgggc gaggagctgc
     6241 tgagccgcac cttaggtctc ctcgcggagg gtggccgttc gcccgtctcc gtcgacgtca
     6301 cgatcgtctg cgaccggcct tcgatcgcaa cgcgacgcct cgagatgcgt gagcgcctcg
     6361 ctcggatcgc cgggctcccg gtcgaacgcg tatccgtgaa ggcgacacgt cccgaggggc
     6421 tcggcctcac cggcgagggc gtcgggtgcc tcgcgctcgc ggtggtcgcg tgagcggcga
     6481 ggtcgtcacg ggacgccgag ccgtcgtcga ggcgctccga gcggggcagg tccgggaggt
     6541 gctcgtcgcg gccggagcgc gcgacaccca ggggctccgg gccgtacgtg aagccgcccg
     6601 cgcggccagg gtgcccgtcc gcgcgaccga tcggcgagcg ctggacgccc tggccgggga
     6661 tcatcgcggc gtcgtcgccc ggacggtggg cggccgcgcc gggcccccgc aactggggga
     6721 gcgcgacctc gccaccctcg cgttcgcgcc ggacgcgatc atcgtcgtcc tggacggcat
     6781 catggatcct cagaacctgg gcgccgcagc gcggacggcg gaggcggcgg gcgccgcggc
     6841 attggtgagc cgaagccgcc gcgcggccgg tgtcaccccc gcggcgatcc gcgcttcggc
     6901 gggcgccctg gagcatctcc cccacgccgt cgtcgccaac atcgcccgtg cgctcgcgcg
     6961 tctgcaggat gcgggattct cggtgatcgg gctggacgac ggtgcctcgt cgtcgatctt
     7021 cgatgaggag tgtccgacgg gccgggtcgc tctcgttgtg ggctcggaag gcgaaggcat
     7081 gtcacggctg gtgcgcgagc ggtgcgacct gctggtggcg cttcccgtga agggtcgcgt
     7141 cggatcgctg aatgcctccg ccgcactcgc agctgccctc tacggcttcg tcctcccgcg
     7201 cgaggtcgtt gcctctcctt catcccctgg ctaggctcgc ggaaccgccg atgcggcatc
     7261 gtcgatgaac gacatcccac caccgccttc gcccggaacc gttcccggtg tgcctacccc
     7321 gcgtccgacg ggcccaccga cgtcgcgccc cggggcggtc accggcgcgg cggcgatcct
     7381 cctgatcgcg ggggcgctcg tcacgatctt cggcctgatg ggcctgacgg gcaacggcgt
     7441 cgacatcgac gcaccgttcc tcgacgggga tgcggccgcg cggatcgtcg cgctggccct
     7501 ggtggtgcag gggatcttat ccctgctcgc cgggtggctg gtcctccgac tccgccccgc
     7561 agggcgggtc ctcgggctcc tgctcgcggc gctcgggatc gtcggtgggc tggcgcaggt
     7621 ccggagaacc gggagcgccg gtctcctcac gttcacgctg aacgcgttcg tgatctacgt
     7681 cctgttgacg tacggattcc tcttcaagcc gcgggagatc ccccggtaga ctcaggtccg
     7741 gccggcgtgg cgcagtccgg tagcgcaagc gatttgtaat cgctaggtcg tcggttcgaa
     7801 tccgaccgcc ggctctcccg aacgacgcgt gcgctgcaaa cgcccccgta gcgtcaggat
     7861 ccgggcagga acacgtggaa cgaggcgccg cccccctcgc gctcctcggc ccaggcattc
     7921 cccccgtgga gctcggcgaa ccgccgcacc agggacaggc cgatgccgac gcccggggcg
     7981 tgcaccgacg aggaccccgg cgcctggcgg aacggctcga aaaccgcctc ccggagctcg
     8041 gacggcacgc cgggaccgtc gtcctcgacg acgaacagga cgccgccgtc gtgcggccgc
     8101 gcgcggaccc agatcttggc ctcctcgggg gtgtgtcgga tgctgttcga caggaggttc
     8161 tcgaggatcc gctccgtctt cgcctgatcg atctggacgc tcccccgctg cacgtcgagt
     8221 tcgatccggc gcccgcgggt gtccatctcc ttcaccgctc gcttgacgaa tccctcgagg
     8281 tcagtggccc ggcgtttcgg tgacacgatc ccgcgctgga gtcgatcgag gtcgagcagg
     8341 tcggacagca gccgttccag cttcttcgcg ttcgccgcga tccttcccac catgtcgagg
     8401 gcgtcgtccg tggggaggtc gagcttggaa cgctccaggg tgagggccga tcccaggatc
     8461 gcggtgagcg gcgtccggag gtcgtgggag accgcctcca ggaacgtgtt cttcatgtcg
     8521 tcgagcatcc ggagacgttg cgctgcttca cgttcgcgct tgaaggcatc ggagtacttc
     8581 cgctcgctgg cggccagtgc gcgctccgca cgcttgcgct cggtgatgtc gaccatcaga
     8641 ccgcggagga tcgtgggtgc gcctccgcgg ccgcggatca cgtgcacgag gtctcggatc
     8701 catcgggggg agccctgatc gctcgggacc cgatactcca atccctcccc aacgccactc
     8761 tcgatcgcgc gcaggagcgc cctggttgcc tcctcgcggt cacgggcgtc gatgtgggaa
     8821 ccccaggtgc ggtcctggtc cggccacgat cccgcgccgc cgccgaacag gtcctccacc
     8881 gcgcctgcga cgaacgtgaa ctccataccc gggatcgacg cttcccagaa ggccgcgtcc
     8941 agtcccgcca cgagctcgtg gaaccgctcg agctcggaca tccgtcgggc gttgccgagc
     9001 gcgagcgacg tcaggtccgc cacgccgcgg atcaggcgga gttcccgatc cccgaagagc
     9061 ccgtcgccgt gccggccgac gacggagatc gtgccggccc cgtccggctc ccacgtcatg
     9121 ggcgcgacca gcaactcccg cggctccttc gagacgaggt agtcgccgtg catcaggggt
     9181 gacgtgcgcg gcaggacgag cggctcttcg ttgtcgaaca cgaccgacag ggcgaccgac
     9241 tcgggaacgt caccgatctc gtgccgaggc cggatcgcgt cgggatcgag cgtgagcagc
     9301 cgtgcgaggc ggaagtcgcc cgtgtgctca tcgcggatgt ggaacgcgac cgccgcttcg
     9361 ccgatcagcg acgacaccgt ctcgatcgct ccctggagga tgtctccgac cgagcgcagc
     9421 tgggtgagct gctcggacag tccgagcagg gccgacgacg tctccgccgc cgtccgctcc
     9481 gcttggagga gcttcgcgtt cgagatcgcg atcgcggcgt gtgaggcgag cacctcgagc
     9541 agccgctggt cctcctcgtc gaaggctccg tagccgaggc tggacaggac gatgacgccc
     9601 tccaccgcgt ccccgacggt catcggcacg gccagcatcg actcgaggat gtcgtctgta
     9661 tcggggatct ggacggcgaa ctccacgagc cgcgcgtcgg cggtgaggag agaggtgcga
     9721 tgctcggcga cccagccagt gatgccctcc ccgaggtgcg tgatcagctc ctcgagggtc
     9781 tcgtgctcgt actcgtagaa gatctcaccg cggaacgcga cggggagcag cgtcgagccg
     9841 tcgtcctgca tcacgtagac gcgacagttg tggtagtcga ggatcgtgtg gagctcgtcc
     9901 gtgatcgcgg cggcgatctc ggagacgtcg tcgaggctgt tcagggtggc cgccatgctg
     9961 tggagcatgt tcaactgcgc cacgaagcgg aggtccgccg acggggcggc gttctcgccg
    10021 atctcttcgc ttcgcgccat caaccagggc cttcccaacg ccatcctccc acctccgtac
    10081 cgtcgggacc ggagcgccgg tgcttgaacc ctgcacgccg cgggctaccc tcgggccatg
    10141 ggagatcgga ggcgttccgc acgtccgggg tcggaggtcg cctccgaccc tgcctcggaa
    10201 ccctccgtgg cgctcgacga gcgatcgcgc gaggtcctcg acttcgaacg ggaagcctgg
    10261 aagctcaccg tgcccaagca gcgcgcgatc cgcgaacggt tcggcttctc tccatcgcgc
    10321 taccaccagc tactccaccg gatcatcgat cgtccggagg cggtggcgta cgacccgatg
    10381 ctggtccgcc gcttgcgccg cgtgcgcgcc gtgcgccgcc ggtttcggac ggcccagggc
    10441 ctcggggtgc gcgtctgagc cggcgaggag cactcccgcc ggaccgccga agaccgcggg
    10501 ctgcggacga taggatgcgc gggtgaatcc gggcatcgcg cggctcgcga tcgtcatcgc
    10561 gctcgtcatc ggaggggtcg cggtgctcgc caacggattc gacgacgaag gggccgcagc
    10621 cccgcccgag ccatcgacgt cccagtcacc gaccgggacg ccgtccccta gtccgtctcc
    10681 gcaagcctcg gtcgtcccca accagaaggg cgtgctcgtc caggtcctca acggaacgtc
    10741 gacggcgggc tacgccgcgg acttccagga gatgctggtc gatctcgggg agtatctccc
    10801 ggcgggagac gcaggggacg cgcccgacaa gccgatcgtc gactcgatcg tctatttccg
    10861 ggcggacgac aacagggcac agaacgaagc cgacgcgcag ctcttgtcgg acgagtacct
    10921 cggaggtgtt ccggtccagc ggctaccggc cgatctcgcg cgatccggtg cgatcgacgc
    10981 cgccgcggac gtcgtcgtgg tgctcggaga ggaccaagcg ggagagctct gagccgtcac
    11041 gctccgtggc ggggagccac gagcgaatcg accatctcgc agagctcgtt cggcaggaag
    11101 ggcttcgtga ggaacgcgga tgcccccaag ccgtgcgaac gctctcgatc ggctctgagg
    11161 gacttcgcgg tgaggaccag gaccgggatg ttcgtggtct ccgcgtcgcg ctgcaacgct
    11221 tccaggacgt cgtagcccga gatccccggc agcatcaggt cgagcacgat cacgtccggg
    11281 tgccgatcac gggcgatcgc cagggctgtg ctcccgtccg cggcttccag cagctcgtgg
    11341 ccccgctgct gaaggttcaa gcggcagagc agcaagacgt ctggctcatc gtcgacgacc
    11401 agtactcgac ccatctgaac gctgtcctcc ttccggggga gccccgtgga acctacatca
    11461 gtcgaatcat cggaacggag gatggggcag ctggataccc ggaatccggg gttccgagcc
    11521 gcaggcggcg ttgacggggc cggaaggccc tcctacgctc gaaggcgacc gaaggtcgcc
    11581 gaagagtctc gaaggacgtg tcccgtgctg aaggtctccc agaagctgga atacgcgatg
    11641 cgcgccatga tcgagctcgc cgatgccacc cacgcggggg tgctcgtgcc ggcccgcgag
    11701 atcgcggctc ggcagcagat ccccctccgg ttcctcgagc agcagctcgg cgccttgtcc
    11761 aaggcggggc tcgtcgagag cttccggggc ggaggcggcg gatgccggct ggcgcggtcg
    11821 cccgatgagg tcaccgtggc cgagatcgcc gacgcgatcg aggggcagat cttcccgatg
    11881 ttctgcctcg agccgtcgga tcacacgtgc ttccaggact cacagtgtgg gctgcaaggc
    11941 ttctgggctg acgtcgcgag ggccgtacag gccgtgttcg cagagacgac ggtggcggac
    12001 ctcgcggccc gccaccgctc gatgtccccg gggctcgtcc tggtcagccc cgagcagctc
    12061 ctgtctcgtc cctaggacgc ggccacgtcc gccttgccga ggatccgcat gaccttggtc
    12121 ccgcagacgg gacacgtccc tgaagcggcg ttgcgcccgt tcttgagggt gacgaccgtc
    12181 ccctcgaact cacgcttcgc cttgcacttc acgcaatacg cttcgtactg agccactccg
    12241 tgcctccctt cccccatcac gctccgagcc gcctcgatgg gcggctctga gcacgatggt
    12301 ggctctgtcc tcccacctat gcaagcaccc aggcccacac acgtcacccc gggacctccc
    12361 gtacgatgga cggtccacgc cctcacgagg gagcacgagc cgagcaggga gggatcaggg
    12421 tgaccgacgc gcagccgcac atcgacgcgc tcctccagga agaacgggtc ttcccgcccc
    12481 cttcgcgctt cgccgagggg gccgtcgtga gcgatccggc gatctacgag cgcgccgccg
    12541 aggacccgga ggcgttctgg tccgagcagg cgggccggct ggagtggagc cgtccctggg
    12601 acaccgtgat ggagtgggcg cccccgttcg tgaagtggtt cgtggggggc accctgaacg
    12661 cgtcggtgaa ctgtctcgac cgacacgtcg cttccggtgg cggcgagaag gtcgcatacc
    12721 actgggaggg ggagccggga gatacgcgga cgctcaccta ccgccagctc ctggacgagg
    12781 tctgtcggct cgccaacggt ctccgctccc tcggggtgcg cacgggcgac cgggtgaaca
    12841 tctacctcgg gatggtgccc gagctgccga tcgcgatgct cgcgtgcgcg cggatcgggg
    12901 ccccccactc ggtcgtgttc gggggcttca gcgcggactc cctccgcgac aggatcaacg
    12961 atgccgaggc caaggtgctc atcaccgccg acggcgcgtg gcgccgcgga cagatcgtcc
    13021 cgctgaaggc gaacgcggac gaggcggtcg cggactgtcc cagcatcgag cacgtgatca
    13081 cgctccgacg gtgcgagaac gagcacgcgt tcacgcccgg gagggacatc tggtaccacg
    13141 aactgatcgg ggagcagtcg acggaatgcg tgcccgagga gatggacgcg gaggacgtcc
    13201 tctatctcct ctacacgagc ggcaccaccg ggaagccgaa ggggatcacg cacacgaccg
    13261 gcggatacct gacccaggtc gcggcgacgc accggatgat cttcgacatc aaggacgacg
    13321 acgtcttctg gtgcgcggcg gactgcggct gggttaccgg gcactcctac atcgtctacg
    13381 ggccgctcgc gaaccacacg acggggatca tgtacgaggg ggccccggac tggccggaca
    13441 aggatcgctg gtggtcgatc gtggagacgt acggggcgac catcctgtac acggcgccga
    13501 ccgcgatccg cgccttcatg cgatggggga ccgactaccc cgagaagcac gacctctcca
    13561 ccctgcggct cctcggatcg gtgggcgagc cgatcaaccc cgaagcctgg gtctggtact
    13621 ggaaggtgat cggcggcgaa cgcgcaccgg tcgtggacac atggtggcag accgagaccg
    13681 gcgccatcct catcacaccg ctgcccgggc tcacggagct caagccgggt tccgcgacga
    13741 tccccttccc ggggatccga gccgacgtcg tcgacgagca gggtgtcacc gtgcccctcg
    13801 gcggcggcgg ctacctcgtc ctcacccgcc cgtggccggc gatcgcgcgg acgatctggg
    13861 gggatcggga tcgatacgcc gagacctact tctccaggtt cgggccggag atctacgtcg
    13921 ccggtgacgg tgccaaacgg gacgaggacg gatacttctg gctcctcggc cggatcgacg
    13981 acgtcatgaa cgtcgccggg caccggctgt ccacgttcga gatcgagtcg gcgctggtcg
    14041 acgacgacaa ggtcgccgag gcggcggtgg tctcgcggcc ggacgacctc gtcggcgagg
    14101 cgatcgtcgc cttcgtcacc ttgaagaccg gctatgaggg cgacgacgcg atggcgaagg
    14161 agctacgcga gcacgtcgcc gccaagatcg gcaagatcgc gcggccgcac gggatcatct
    14221 tcaccgcaga tcttccgaag acacgatcgg gcaagatcat gcggcggctg ctccgtgatg
    14281 tggcgcacgg gcggaccctc ggcgacgtga cgaccctcgc gaacgcggag gtcgtggagc
    14341 agatccgcga gatgtcggag agtgccccgg ccgacgagtg acgatcgggc ccgggtgaac
    14401 gtctcgcgct cgtcgtggac tactcggcca ggtgccgccg gaagaagtcg atcgaccggc
    14461 gggcatgggc gatcttctct tccacctgca gcgcgtggtg cccgccgccg tacaggtgca
    14521 cgtcgacctc gtgccctcgc gcgcgcaggg catgggcgta caccatcacc tggccgagcg
    14581 ggcagcggct gtcgtgctcg ccggcgatca gcagcatcgg cgcctggacg cggtccacgt
    14641 acgtcatcgg gttccgctcg cgatagagct ccgggagatc catcggggac ccccccatgg
    14701 tggcgacgtc ccaggcgcgg agcgtcggcg cgctctcgta atgggcggcg acgtagtcgc
    14761 cgacggggat ccccgcgcag gccgcgcgcc atcggtcggg gtggagcccc gcgatcagcg
    14821 tcgtcaggta cccgccccac gaccagcctt cgaggaacag cgcccgtggg tcggcgaagc
    14881 cttcggcgac cacgtgatcg acgcccgcaa cgatgtcctc ggtctccggg aacccgatgt
    14941 tccctttcag ctcctcgcgg aatgccttgc cgcgaccgga cgaccctcgg tagttgacct
    15001 gcaggaccgc gaacccctcg tcgacgaagg cctgcacccc cggatcccac agatccgtgt
    15061 ggtgccactc gggcccgccg tggatggatg cgatcgtcgg gaacggcccg gaacctgtcg
    15121 gggtggtcag gaagccggtg atcaggtcgc gcgaggggtt ggtccaggtc agccatcggc
    15181 tcctggtccc cggcggcgga ggatcgaccg gcaactcgag gatcgcggct ccggcggcat
    15241 cctggacgga cggaggctcg acgctcgagt cgccgcggta ccagagatcg ccgcccgagc
    15301 ggaagccggc ctcgtagatc gtcccctccc tcctgacggt ctcgtccacc gatccggcca
    15361 cggggtcgat cgtgaagagc cggttcacgc cctcccccgg atcgttgtgc gcgaggatgc
    15421 gggatccgtc gagggtccat cccaatggga tcaccggacc ggtcacgtcg gagagatcga
    15481 ggtcgagccg ctcccgcgtg tcgaggtccc agatcgctgg gcgctcgatc cccgagcgct
    15541 cctgcgtgaa gaggagccgc cgggcgcccc cggcaggcga ccaggccacc gcctccatcc
    15601 gcagcccctg atcgacctgg tcgccagcgg actcgccgga gcgggcgtcc agcacccgga
    15661 gggcctgccg gtcgatgtcc ccgttctcgg agtggtggat gcacacgagg gcgccgtcgg
    15721 gagaaagccc gccgcctccc tgcggccatt cgctgcccac gccggcgggc tccgggttcc
    15781 ggtagatctg gcggggagct tccgaaccca tcgcgacgaa cacgtggtag tcctcgtcgg
    15841 tcgcgaagcc ggcggccatc ccgccgtcga tcagggacag acccatcatc cactcgtcgt
    15901 ggaacccgcg gaagagcggc tgcgcctccc cgccctcgaa gggcgtcagc atccacctgc
    15961 cgcgctcgtt cccgatctcg tcgtaccacc acaccacgcc ggagccgtcc ggcaggatgt
    16021 gcgcctcctc ggcgcccacg ccgcccgtgg acacacgccg gcgctcgttc gctcgtacgt
    16081 cccaggccca ggcttgcgtg ctgccgtcct ccgtggtgac cgccgccgcc cggtccggtg
    16141 ccgatgaggc ccacaccacc tgctcgatcg acggatgggt gaagcggagg gtccagccgg
    16201 ggcgcgcgct cacggcgctg aggataggcc cccgacgccg tgaggccacg caggggggtg
    16261 tttcgtcggt ttgccactcg cgcggcgagg cccctagaat ccttagcact cgggtctccc
    16321 gagtgctagc agtcggaagc ctcgcctggg aacgcgtcgc ggacgcccgt tccgggacga
    16381 ggaccgagac caggaagccg aacagggaga aggaggtcga gggggatggc tcccaagatc
    16441 atcagcttcg acgaggacgc gcggcgcgcg ctcgaacgag gcatggatca gctggccaac
    16501 gccgtgaaga tcacgctcgg tccgaagggc cgcaacgtgg tgctcgagaa gaagtggggg
    16561 gcgccgacga tcacgaacga cggcgtgtcc atcgccaagg agatcgagct cgaggacccg
    16621 ttggagaagg tcggtgcgga gctggtcaag gaggtcgcga agaagaccga cgacgtggcc
    16681 ggcgacggca ccaccaccgc cacggtcctc gcgcagtcga tcgtgaagga aggtctgcgc
    16741 aacgtcgcgg ccggcgccaa cccgatgtcg atcaagcgcg gcatcgagaa ggccgtcgag
    16801 ctcgccgtcg aggccatcaa gaactccgcg aaggaggtcg actcgaagga cgacatcgct
    16861 cacgtgggcg cgatctccgc ggccgacccc gagatcggcg agaagatcgc cgaggccgtc
    16921 ga
//
LOCUS       DASUYD010000237         6478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1727461, whole genome shotgun sequence.
ACCESSION   DASUYD010000237 DASUYD010000000
VERSION     DASUYD010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727461"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..972)
                     /locus_tag="VF195_12530"
     CDS             complement(<1..972)
                     /locus_tag="VF195_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581682.1"
                     /translation="MLRTGRWRVRAALIASSALLLSATVMAPGGSAAPLCDAPPDVFP
                     VADLEAGMMATGWTVVEGTDPVSFEVEIVGVLPDGIAPGVDFILVHVSGPVIDATGGI
                     AFGFSGSPVYIGSELVGSISYGFFGADQTFGGVTPAEGMVRLLEYADGTGALSANSTA
                     KAADTVRVPLRLREAAARTVNVAISSIPSDAEQLRVPLAVSGLTDRGLRRFSRTLRLG
                     GLSVLPYRSGAVGVTELAATPLEAGDAVAGAVSVGDLTVAGVGTTTATCDELLLAFGH
                     PFFWEGRAGRFPMGMYGAEVVQVIPDPSQVFGAFKIANLTELHGIIDQ"
     gene            1200..2294
                     /locus_tag="VF195_12535"
     CDS             1200..2294
                     /locus_tag="VF195_12535"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX6581683.1"
                     /translation="MSRRVIPGLESPDPVLESEVRTRLDRVEEALHKALAEADSQLLS
                     ATANYLLAAGGKRFRPMLALLSGYFGDPADPRLIPASVSLELVHVATLYHDDVIDEAE
                     IRHGVPAANVRWDNTVAILTGDYLFARASEVGADLGPEVCRILAQTIAVLCDGQIREV
                     DASRRLDQTEANYLEVIRRKTGALIAASCRLGAMMSDAPREHVDILEGFGESLGLAFQ
                     LSDDIMDITSSQLELGKEPGVDMKEGVYTLPVLHALRTSPDREELARILSTGVDGGPL
                     DRALEIVSGGDSIEHARAAVRDEVARAVALVERLPAGPAQHALVQLARFLSARCGADA
                     WAGDVASDGPSGSEGDPARIRAREDFHKRA"
     gene            2287..2652
                     /locus_tag="VF195_12540"
     CDS             2287..2652
                     /locus_tag="VF195_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012887432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX6581684.1"
                     /translation="MPDYYSSYGVVLAVVAAGAILVAVAFTAAKLIAPHRPLPAKLTT
                     YECGIDPVGEGWSQSQIRYYVFGFLFVIFDVEAVFLFPWARVFESLGWAAVVEMGIFI
                     AILATGLLYAWRKGVVRWA"
     gene            2679..3149
                     /locus_tag="VF195_12545"
     CDS             2679..3149
                     /locus_tag="VF195_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005313711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEX6581685.1"
                     /translation="MQPVKFLLNWGRRYSLWVMNFGLACCAIELIATSTSKHDFIRLG
                     VIPFAHGPRQADLLVVAGTLTDKMAPALKRVYDQIPDPKYVISFGSCSNCGGPYWDSY
                     SVTKGVDQIVPVDVYVPGCPPRPEALLHGIVKLQEKIKDEDIQEKWASGHSAVS"
     gene            3127..3648
                     /locus_tag="VF195_12550"
     CDS             3127..3648
                     /locus_tag="VF195_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX6581686.1"
                     /translation="MATALSPEDVESRLRARFGQDFLAFEEQHGHAVATVGVGRYREV
                     CRFLRDEPEFACDYCDFTGGVDFGPERGFEVVTHLFSTTHRHNVRIKVRLPHEDPVCP
                     TVSDLFPTSDWHERETMEMFGIRFEGHPKPVKLLLSEPFEGQPLRKDFPLMTREAKPW
                     PGLVEGEDVEDDE"
     gene            3684..4652
                     /gene="nuoH"
                     /locus_tag="VF195_12555"
     CDS             3684..4652
                     /gene="nuoH"
                     /locus_tag="VF195_12555"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019632703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEX6581687.1"
                     /translation="MFSNFWVVLLLKTIAVMGFFLVVPLGVGYVEHKGLAHMQARLGP
                     MEAGRFHGWAQLVADGVKFIQKEDVIPAAADRNVFSLAPAVAMIPYIAILVVLPISDS
                     WFAENLDVGIFFVLAMSSVSVIGVMMAGWSSANKFSLIGALRAAAQLIAYELPLVIAA
                     AAIVMQAGTLSMIGIVEAQQDLWFIIPQLPGFLIFMIAAFAELQRPPFDMPIADSEII
                     FGAYTEYTGLKFAFFLLAEYGGIVAFSGIAAVLYLGGYQPLPGIPIPGPLMMMGKIGA
                     LAFFFIWLRATYPRLREDQLQRFSWLALIPLSLALIMGVAVAKVAF"
     gene            4653..5156
                     /locus_tag="VF195_12560"
     CDS             4653..5156
                     /locus_tag="VF195_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015578346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEX6581688.1"
                     /translation="MPEQTRKRGTGLGLAKGLSVTLKTMLQPAVTQQYPRVKPDLPPR
                     TRGVIALKEANCTVCYKCSRECPDWCIYIDAHKETHDPAGGGRARSAKVLDRFAIDYA
                     LCMYCGICVEVCPFDALFWSPEFEYGEYDLDLMTHEKERLEEWTYTVLPPPALEDGAV
                     PTEEQAS"
     gene            5153..5653
                     /locus_tag="VF195_12565"
     CDS             5153..5653
                     /locus_tag="VF195_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017948751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEX6581689.1"
                     /translation="MTAQEYAFAVIAAVGSISAIAVVTARNVVHAALYLVVALLSVGA
                     TFLLLGAEFAGWVQILIYVGAIVILFLFGLMLTKAPIGRDTLDNAHRWVGLLVAAGVF
                     AGLAFLIQDAFPLDAEPFEVFHGSTGVVGESMFRDFVLPFEAISFLLLAALIGAIVLA
                     RKDEGN"
     gene            5655..5957
                     /gene="nuoK"
                     /locus_tag="VF195_12570"
     CDS             5655..5957
                     /gene="nuoK"
                     /locus_tag="VF195_12570"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEX6581690.1"
                     /translation="MIRLPFVLLFSAFLFSAGVYGVLARRNAVMVLMAIELMLNAVNV
                     NLVAFSSSLHDVGGQIFALFVIAVAAAEVGIGLAIVILLFRNRASINVDEVNLLKW"
     gene            5951..>6478
                     /locus_tag="VF195_12575"
     CDS             5951..>6478
                     /locus_tag="VF195_12575"
                     /inference="COORDINATES: protein motif:HMM:NF012868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581691.1"
                     /translation="MVAVVFTPIAAAAAATTGVATGAIQYAWVIALLPFIAAPLILFF
                     GKRTPGQGAVFGIAALGAGFVMALAVLFHFVTGGGTYAPEGIEWFEIGPLHLEVGQYV
                     DGLTAVMLVVVTAVSLAVHVYSLGYMHGDVRFTWFYVVLSLFTGAMLTVVIADNLLML
                     LVGWEIMGVCSYLLIG"
BASE COUNT          939 a         2223 c         2170 g         1146 t
ORIGIN      
        1 ctggtcgatg atgccgtgca gctccgtgag gttcgcgatc ttgaacgcgc cgaagacctg
       61 gctcgggtcg gggatcacct gcacgacctc ggcgccgtac atccccatgg ggaaccggcc
      121 agcgcgaccc tcccagaaga acggatgccc gaaggcgagc aacagctcat cgcacgtggc
      181 cgtcgtcgtt ccgaccccgg cgacggtcag atcgcccacg ctcaccgccc cggcgacggc
      241 gtcgcccgcc tcgagcggcg tcgcagcgag ctccgtcacg cccacggcgc ccgaccgata
      301 ggggaggacc gacaggccgc cgagccgcag cgtgcgcgag aacctccgca ggccgcgatc
      361 ggtgaggccc gaaaccgcga gcgggacgcg cagctgttcg gcgtcgctcg ggatcgacga
      421 gatggcgacg ttcacggtgc gcgccgccgc ctcgcgcagc cggagcggca cccggaccgt
      481 gtcggccgcc ttggccgtcg agttcgccga cagcgctccg gtcccgtccg cgtactcgag
      541 cagccggacc ataccctccg ccggcgtgac gccaccgaac gtctgatcgg ctccgaagaa
      601 cccgtacgag atcgacccga ccagctcgga tccgatgtac acgggcgacc ccgagaagcc
      661 gaaggcgatt ccgcccgtcg cgtcgatcac cggtccggag acgtgcacca ggatgaagtc
      721 cacaccgggg gcgatgccgt cggggaggac gcccacgatc tcgacctcga acgagaccgg
      781 atcggtcccc tcgacgaccg tccagcccgt cgccatcatg ccggcctcga ggtcggccac
      841 cgggaacacg tccggcggtg cgtcgcacaa cggagccgcc gagccaccgg gggccatcac
      901 ggtcgccgac agcagcaagg cagacgaagc gatcaaggca gcgcgaacgc gccaccgtcc
      961 agtccgtagc atcggatcca ccccccggtt ccgtgtcgac gcaaccctat ccccttcgcg
     1021 ccgagcttgc tcgagccttc cggggcttat gccccggttg ctcgttgatg tgacgcctgg
     1081 ggaagtcctg gggttccatc cacccgaggt acgctgctcg tgaaatgctt cacgagcagg
     1141 ctggacccct tgggagggcg gatggcggga cccggacgga cgtcggcgag ggcgggacgg
     1201 tgagtcggcg tgtgatcccc ggtctggagt cgcccgaccc cgtgctggag tcggaggtcc
     1261 ggacgcgcct cgaccgcgtg gaggaggcgc tccacaaggc gctcgcggag gccgactcgc
     1321 agctgctgtc cgccaccgcg aactatctcc tcgcggcggg cgggaagcgt ttccgaccga
     1381 tgctcgccct cctgtcgggg tacttcggcg atcccgcgga ccctcggctg atccccgcct
     1441 ccgtgtccct cgagctcgtc cacgtggcga ccctgtacca cgacgacgtg atcgacgagg
     1501 ctgagatccg ccacggcgtt ccggccgcga acgttcgctg ggacaacacc gtcgccatcc
     1561 tcaccgggga ctacctgttc gcccgcgcgt ccgaggtcgg cgcggacctc ggcccggagg
     1621 tctgccgcat cctggcgcag acgatcgccg tgctctgcga cggacagatc cgtgaggtcg
     1681 atgcctcccg ccgcctcgac cagaccgagg ccaactacct cgaggtgatc cggcgcaaga
     1741 cgggcgcgtt gatcgcggcc tcgtgccggc tgggggcgat gatgtcggac gcgcctcgcg
     1801 agcacgtgga catcctcgag gggttcgggg aatcgctcgg cctcgccttc cagctctcgg
     1861 acgacatcat ggacatcacg tccagccagc tcgagctcgg caaggagccg ggcgtcgaca
     1921 tgaaggaggg ggtctacacc ctgcccgtgc tccacgccct ccggacgtca cccgatcggg
     1981 aggagctggc gcggatcctg agcacgggtg tggacggtgg gccccttgac cgcgcgctcg
     2041 agatcgtctc gggcggcgac tcgatcgagc acgcccgcgc ggccgtgcgc gatgaggtcg
     2101 ctcgcgcggt cgccctcgtc gagcggctcc cggcggggcc cgcgcagcac gcgttggtgc
     2161 agctcgcgcg gttcctttca gcgcgctgcg gggccgacgc gtgggccggc gatgtggcct
     2221 cggacggacc atcagggagc gaaggggatc ctgctaggat ccgcgctcgg gaagactttc
     2281 acaagcgtgc ctgactacta ctcgtcctac ggtgtcgtgc tggcggtcgt ggccgcggga
     2341 gcgatcctcg tcgcggtggc gttcacggcc gcgaagctga tcgcgcccca ccgtccgctt
     2401 cccgccaagc tcaccacgta cgagtgtggg atcgaccccg tcggtgaggg ctggtcgcag
     2461 agccagatcc gctactacgt cttcgggttc ctcttcgtga tcttcgacgt cgaggccgtg
     2521 ttcctcttcc cctgggcgcg cgtcttcgag agtctcggct gggccgcggt ggtcgagatg
     2581 ggcatcttca tcgcgatcct cgccaccggc ctcctctacg cctggcgcaa gggagtcgtg
     2641 cggtgggcct gatcaccgag aagacaccgc tccccaagat ccagccggtc aagttcctgc
     2701 tgaattgggg ccgccgatac tccctgtggg tgatgaactt cggcctcgcg tgctgcgcga
     2761 tcgagctgat cgccacgtcg acctccaagc acgacttcat ccggctgggc gtgatcccgt
     2821 tcgctcacgg cccccgccag gccgacctcc tcgtcgtcgc cggaacgctc accgacaaga
     2881 tggcccccgc cctcaagcgg gtctacgacc agatcccgga cccgaagtac gtgatctcgt
     2941 tcggctcctg ctcgaactgc ggcggtcctt actgggactc ctactcggtc accaagggcg
     3001 tcgaccagat cgtgccggtc gacgtctacg tgcccggctg cccgccccgc cccgaagccc
     3061 tgctgcatgg gatcgtgaag ctgcaggaga agatcaagga cgaggacatc caggagaagt
     3121 gggcgagtgg ccacagcgct gtctcctgag gacgtggagt cgcgtctgcg ggcgcgcttc
     3181 gggcaggact tcctcgcgtt cgaggagcag cacggccacg ccgtcgccac cgtcggcgtg
     3241 ggtcggtacc gcgaggtctg ccgcttcctg cgggacgagc cggagttcgc gtgcgactac
     3301 tgcgacttca ccggcggtgt cgacttcggc cccgaacgcg gcttcgaggt ggtgacgcac
     3361 ctgttctcca cgacgcaccg tcacaacgtc cggatcaagg tgcggctccc gcacgaggac
     3421 ccggtctgcc cgacggtctc cgacctgttc cccacctccg actggcacga gcgagagacg
     3481 atggagatgt tcgggatccg cttcgagggc cacccgaaac cggtcaaatt gctcctgtcc
     3541 gagccgttcg agggccagcc gctccgcaag gacttcccgc tgatgacccg tgaggcgaag
     3601 ccctggcccg ggctcgtgga gggggaagac gtggaggacg acgagtgatc ctcgccgaga
     3661 ccggcgtctc ggtcttctcc tcgctgttct cgaatttctg ggtcgtcctg ctgctcaaga
     3721 cgatcgccgt gatggggttc ttcctcgtgg tccccctcgg cgtcgggtac gtggagcaca
     3781 agggcctcgc ccatatgcag gcgcggctcg gaccgatgga ggcgggcagg ttccacggct
     3841 gggcgcagct cgtcgcggac ggtgtgaagt tcatccagaa ggaggacgtg atccccgcgg
     3901 ccgcggaccg gaacgtgttc tccctcgcac cggcggtggc gatgatcccc tacatcgcga
     3961 tcctcgtcgt cctgccgatc agcgactcgt ggttcgccga gaacctcgac gtcgggatct
     4021 tcttcgtcct ggccatgtca agcgtctccg tgatcggtgt gatgatggcg ggctggtcct
     4081 ctgcgaacaa gttctcgctg atcggcgcgc tgcgggcggc cgcgcagctg atcgcctacg
     4141 agctgccatt ggtgatcgcc gcggccgcca tcgtgatgca ggccggcacg ctgtcgatga
     4201 tcgggatcgt cgaggcgcag caggacctct ggttcatcat cccgcagctc ccgggcttcc
     4261 tgatcttcat gatcgccgcg ttcgccgagc tgcagcggcc gccgttcgac atgccgatcg
     4321 ccgattccga gatcatcttc ggcgcctaca ccgagtacac gggcctgaag ttcgcgttct
     4381 tcctgctcgc cgagtacggc gggatcgtgg ccttctcggg gatcgcggcg gtcctgtacc
     4441 tcggcggcta tcaaccgctt cccggcatcc cgatccccgg gccgctgatg atgatgggca
     4501 agatcggggc attggccttc ttcttcatct ggctgcgggc gacgtatccg cggctccgcg
     4561 aggaccagct ccagcgcttc tcgtggctcg ccttgatccc cctgtccctg gcgctgatca
     4621 tgggcgtggc ggtggcgaag gtggcgttct agatgccgga gcagacgcgc aaacgcggga
     4681 ccgggctcgg gctcgccaag ggcctgtcgg tcacgctcaa gacgatgctg cagccggcgg
     4741 tcacgcagca gtatccgcgc gtcaagcccg acctccctcc tcggacccgc ggcgtgatcg
     4801 cgctcaagga ggccaactgc accgtctgct acaagtgctc ccgagagtgc cccgactggt
     4861 gcatctacat cgacgcccac aaggagacgc acgacccggc gggcggcggt cgcgcgcgct
     4921 ccgccaaggt gctcgaccgc ttcgcgatcg actacgccct gtgcatgtat tgcgggatct
     4981 gcgtcgaggt gtgtccgttc gacgccctgt tctggagtcc ggagttcgag tacggcgagt
     5041 acgacctcga cctgatgacc catgagaagg agcggctgga ggagtggacg tacacggtcc
     5101 tccctccgcc ggcgctcgag gacggcgccg tgcccacgga ggaacaggct tcgtgaccgc
     5161 tcaggaatac gccttcgcgg tcatcgcggc ggtggggtcg atcagcgcga tcgcggtcgt
     5221 gaccgcccgc aacgtcgtgc acgccgccct gtacctagtc gtggcgctcc tgtcggtcgg
     5281 agcgacgttc ctcctcctcg gcgccgagtt cgccggctgg gtgcagatcc tgatctacgt
     5341 cggcgcgatc gtgatcctgt tcctcttcgg actgatgctg acgaaggcgc cgatcggccg
     5401 ggacaccctc gacaacgccc atcgatgggt ggggttgttg gttgccgccg gagtgttcgc
     5461 cgggctcgcc ttcctgatcc aggacgcgtt ccccctcgat gcggagccgt tcgaggtctt
     5521 ccacgggagc accggagtgg tgggggagtc gatgttccgc gacttcgtcc tcccgttcga
     5581 ggcgatctcg ttcctgttgc tcgcggcgct gatcggagcg atcgtgctcg cgcgcaagga
     5641 cgaggggaac tgacgtgatc cggctcccgt tcgtgctcct gttctcggcc ttcctcttct
     5701 cggcgggcgt ttacggtgtc ctcgcccgca ggaacgccgt gatggtgctg atggccatcg
     5761 agctgatgtt gaacgccgtg aacgtgaacc tggtggcgtt ctcgtcatcc ctgcacgacg
     5821 tgggcggcca gatcttcgcg ctcttcgtga tcgccgtcgc cgcggccgag gtcgggatcg
     5881 ggctcgcgat cgtgatcctg ctgttccgga accgggcctc gatcaacgtg gacgaggtga
     5941 acctcctcaa gtggtagcgg tcgtcttcac tccgatcgct gccgcagcgg ccgcgaccac
     6001 tggcgtcgca accggcgcga tccagtacgc gtgggtgatc gcgctcctcc cgttcatcgc
     6061 ggctcctctg atcctcttct tcgggaagcg gacgccgggg cagggtgcgg tcttcggtat
     6121 cgccgcgctc ggagccggct tcgtgatggc cctcgccgtg ctcttccact tcgtgacggg
     6181 cggcggtacg tacgcacccg aggggatcga gtggttcgag atcggaccgc tccatctcga
     6241 ggtggggcag tacgtcgacg gcctcacggc ggtgatgctc gtcgtggtca cggccgtgtc
     6301 gctcgcggtc cacgtctact cgctgggcta catgcacggg gacgtgcggt tcacgtggtt
     6361 ctacgtcgtc ctgtccctgt tcacgggcgc gatgctcacc gtcgtcatcg cggacaacct
     6421 gctgatgctg ctggtcggct gggagatcat gggcgtctgt tcctacctgt tgatcggg
//
LOCUS       DASUYD010000238         6570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_184081, whole genome shotgun sequence.
ACCESSION   DASUYD010000238 DASUYD010000000
VERSION     DASUYD010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184081"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF195_12580"
     CDS             complement(<1..546)
                     /locus_tag="VF195_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX6581692.1"
                     /translation="MISLQRVTKTYRNGVTALEDVSVEIDKGEFVFVVGPSGSGKSTM
                     IRLLLKQEEASRGDVFVAGKNLSRITSFKVPHLRRNIGTVFQDYKLLQDKTVFENVSF
                     ALEVIGKAKHVIDQRVPEILEYVGLGDKLNAYPDELSGGEQQRVSIARACVNRPLVLL
                     ADEPTGNLDPNTSVDIMRLLDK"
     gene            635..3064
                     /locus_tag="VF195_12585"
     CDS             635..3064
                     /locus_tag="VF195_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581693.1"
                     /translation="MDAADASRLASRGARRTAVWLGPALIVVSVLFALRGFVVGGALT
                     NQHPDLLTFWLPRWSFLGDSIATGRIPIWNPYEMAGYRFAADPQSGWLYAPPMLLFST
                     LSPGLALRSFIVFNPLLAGLGLVAFLRMESIGRSAATAGGLVLAMTMSTSTIAISLPF
                     AGALAWTTVLLVAAAGSRRSQRWSGRLAWLALGGVAWSQVAAAHMSHGLVIATAFATA
                     YLAAGAVADARVARTSARSVVGPVALFLVVLPLAALPILVPHVDLLGGSSLQGGYGAL
                     GDAGQSEVGDPLGSDGVWAAWPLGSASAPGAYAGAVALACVLVAARANRLRSLVIAVG
                     GLALVAYVLTSSFLVGSRWFRSLMLEIPFGDVYLHNPGRLRYVTVLALPILAAAGLQG
                     LVERPLSRRGGMAWLGAAVSVFFLWPLVAGGSAVRFGMFAAALLVAGPVLFGFATRRW
                     RWASATALIGLLSIELLASAVYAQTYDGGTVFTGLETGEHPALVPQVLRFPSLSEDDY
                     LVATPIVEHIRRRPGRYATWAPPAAFYEKGYLWMKSHSDWPALAPSRGSLFGIPDALG
                     YNPVQLPRYWRYIRSTNELSVFYNASVLNVPSPEDVRVLGLRYLVVPTGIDPPVGGRV
                     VEEADGYSLWELDDAQPLSTVTSMVEFVDGLASAMDEVTTSGFDPAVEAVLESSTDVF
                     TCVGECPPTAPAVTTVISGSGLVIQVEESEGGVLTIRNAYDAGWRAVVDGRAATTIPV
                     DGFLQGVVLPPGAREIVLTYHDDAVILGLALGAGFWLVLLSASIVALAFERRRPGGDG
                     VRRPRPPPPDA"
     gene            complement(3030..4146)
                     /gene="prfB"
                     /locus_tag="VF195_12590"
     CDS             complement(join(3030..4079,4081..4146))
                     /gene="prfB"
                     /locus_tag="VF195_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEX6581694.1"
                     /translation="MSDHTERIGKLRDRILAAKEFLDVEGKRAQLVDVERDASDPSLW
                     DDPVRGQQLTQRLARLRDQLDRHERLERSLGDLEATDELLEDETDHELMSELEAGLER
                     LERELQGVELTALLSGPYDDHDAIATLQAGAGGTESQDWTDMLLRMYGRWAEKLGFRL
                     EIDEIHHGEEAGIKSATFMLHGTNAYGLLSAERGVHRLVRISPFDSQKRRHTSFAALD
                     VIPLLEAAEEEEVEIPDEDLRIDVFRSSGPGGQSVNTTDSAVRITHLPSGIVVACQNE
                     RSQLQNRAVAMRILKARLAELERRKRDEEMDVLRGERKAVDFGSQIRSYVLAPYQMVK
                     DHRTEFEMGDPSQVLGGDIDRFIEAELRRRAAVGEAV"
     gene            4369..4593
                     /locus_tag="VF195_12595"
     CDS             4369..4593
                     /locus_tag="VF195_12595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581695.1"
                     /translation="MSTRSQALSPGISWNATRAASVLLVTIIAVIALLFWPSIESARP
                     TAPAARESTGLAAPHQPIVVDGRVCVQCLP"
     gene            4765..5202
                     /locus_tag="VF195_12600"
     CDS             4765..5202
                     /locus_tag="VF195_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581696.1"
                     /translation="MSAQGATLGRGTVHVPIWTVAVVVLAAATAALIGFSTLDTSDTI
                     APARPLVTVTDAQRLANSTAAIREQGAVAPVAVGISHVPPAAVVPVGYVGLENPGAYV
                     PRSAYANGLENPGAYAPSEGAPAVTAPASAGIMVNGEICHQCR"
     gene            complement(5290..>6570)
                     /locus_tag="VF195_12605"
     CDS             complement(5290..>6570)
                     /locus_tag="VF195_12605"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX6581697.1"
                     /translation="GRKGGVTVATNMAGRGVDILLGGNPEFLARQEMAARDFDNDRYL
                     LFEMDEEERAAYEAEYEPIFAKFRAQTDAEHDEVVELGGLYVLGTERHESRRIDNQLR
                     GRSGRQGDPGASLFYLSLEDDLMRMFASDRVSSIMERLKWPEDEPIEAKMVTRAVQNA
                     QGQIEELNYERRKNVLKYDEVMNGQRTVIYGERRKILEGEDLSEEALGFVRDVVEATV
                     ANYCPADVFAEEWDREGLLTALTEVYPVSLSKDQVDEIADTESLQAAFVEDALTAYAA
                     KEASLGVEMMRELERVVLLNIIDTRWREHLYEMDYLQEGIYLRSYAQKDPITEYRREA
                     FDMFEELTSTIRAEFVRYIYRVELVRPEQPQRRAAAQKVAAVRERHGDDEAARPSGAQ
                     DPNPNQVISDKVPRNAPCPCGSGRKYKKCHGAVA"
BASE COUNT          895 a         2307 c         2220 g         1148 t
ORIGIN      
        1 cttgtcgagg agccgcatga tgtcgacgga ggtattgggg tcgaggttcc cggtgggctc
       61 gtcggcgagc agcacgagcg gtcggttcac gcacgcccgc gcgatcgaga cacgctgctg
      121 ctcgccgccg gagagctcgt cgggatacgc gttcagcttg tcgcccagcc cgacgtactc
      181 gaggatctcc gggacacgct ggtcgatcac gtgtttcgct ttcccgatca cctcgagggc
      241 gaacgagacg ttctcgaaca cggtcttgtc ctgcaggagc ttgtagtcct ggaagaccgt
      301 gccgatgttc cgccgcaggt ggggcacctt gaacgaggtg atcctcgaga ggttcttgcc
      361 ggcgacgaac acgtcgccac gtgaggcctc ctcctgcttc aggaggaggc ggatcatcgt
      421 ggatttcccg gagccggacg ggcccacgac gaagacgaat tcgcccttgt cgatctcgac
      481 cgagacgtct tccagggcgg tgacgccgtt ccggtaggtc ttggtgacgc gttgaaggct
      541 gatcatggcg tgttgaatgc gaccgggaga tggggtcggc caaccctacc agaggtcttc
      601 cgcgagagcg gggaaggtgc cgtaggcttc ctccgtggac gccgcggatg cgagccggct
      661 cgcgtcacgc ggggcacggc gcacggccgt ctggctcgga cccgccctga tcgtcgtgtc
      721 cgtcctgttc gcgctccggg gtttcgtcgt cggcggcgct ctcaccaacc aacatccgga
      781 cctcctcacg ttctggctcc cgcgctggtc gttcctcggg gactccatcg ccaccggccg
      841 gatcccgatc tggaacccgt acgagatggc gggataccgc ttcgccgccg acccgcagag
      901 cggatggctg tatgcgccac cgatgctcct gttctcgacg ctgtcgcccg ggctcgccct
      961 ccgctcgttc atcgtcttca acccgctcct cgccggcctg ggactcgtcg cgttcctgag
     1021 gatggagtcg atcggtcgga gcgccgcgac ggcgggcgga ctcgtgctcg cgatgacgat
     1081 gtcgacctcc acgatcgcga tctcgctgcc gttcgccggc gcgctcgcgt ggacgaccgt
     1141 cctcctggtc gccgcggccg gctcccgccg gagccaacga tggtccgggc ggctcgcgtg
     1201 gctggcgctc ggcggcgtcg cgtggagcca ggtggccgcc gcgcacatga gtcacgggct
     1261 cgtgatcgcg acggcgttcg cgaccgccta cctggcggcc ggagccgtcg ccgacgcccg
     1321 cgtcgcacgg acgagcgcgc gatccgtcgt cgggccggtg gcgctgttcc tggtcgtgtt
     1381 gcccctggcc gcgctgccga tcctcgtgcc gcacgtcgac ctcctaggtg gatccagcct
     1441 gcagggtggc tacggcgcgc tcggcgatgc ggggcagtcg gaggtcggcg atccgctcgg
     1501 ttcggacggc gtctgggcgg catggccgtt ggggtccgcc tcggcgccgg gcgcctacgc
     1561 gggcgcggtg gcgctggctt gcgtcctcgt agcggctcgc gcgaaccggc tccggtcgct
     1621 ggtgatcgcg gtcggagggc tcgcgctcgt cgcctacgtg ctgacctcct cgttcctggt
     1681 cgggtcccgg tggttccgct cgctcatgct cgagatcccc ttcggggacg tctatctcca
     1741 caacccgggc cgcctccggt acgtgaccgt cctggcgctg ccgatcctgg ccgccgccgg
     1801 actgcagggg ctggtggaac gaccgctctc gcgtcgcggc gggatggcct ggctcggagc
     1861 cgccgtctcc gtgttcttcc tctggccgct cgtcgccggt ggcagcgccg ttcggttcgg
     1921 gatgttcgcc gcggcgctgc tggtggcagg accggtgctg ttcgggttcg ccacgcgccg
     1981 ctggcgatgg gcctcggcga ccgcgctcat cggcctgctc tcgatcgaac tgctcgcgag
     2041 cgccgtctac gcgcagacgt acgacggggg cacggtgttc accgggctgg agaccggcga
     2101 gcatcccgca ctcgtgccac aggtcctgcg cttcccatcg ctgagcgagg acgactacct
     2161 ggtcgccacg cccatcgtcg aacacatccg tcgccgcccg ggccgctacg ccacctgggc
     2221 cccgcccgcg gccttctacg agaaggggta cctatggatg aagtcccact ccgactggcc
     2281 ggcactcgct ccgagccgag gctcgctctt cgggatcccc gacgccctcg gctacaaccc
     2341 cgtgcagctc cctcgctact ggcgctacat ccgcagcacg aacgagctgt cggtcttcta
     2401 caacgcctcg gtcctcaacg tgccgtctcc cgaagacgtc cgcgtgctcg ggttgcggta
     2461 cctggtcgtc cccaccggga tcgacccccc cgtcggcggt cgtgtggtcg aggaagccga
     2521 cggctactcg ctgtgggagc tggacgatgc gcaacctctc tcgaccgtca cctcgatggt
     2581 ggagttcgtg gacggcctgg cctcggcgat ggacgaggtc acgacgagcg gcttcgatcc
     2641 agcggtggag gcggtgctcg agtcctcgac ggacgtcttc acgtgcgtcg gcgagtgccc
     2701 tcccacggcc ccggcggtca ccacggtgat ctccgggtcc gggctggtga tccaggtcga
     2761 ggagtccgag ggaggcgtcc tcacgatccg gaacgcctac gacgccggct ggcgagcggt
     2821 cgtggacggt cgagcggcga ccacgatccc ggtggacggg ttcctccagg gagtggtcct
     2881 ccccccggga gcgcgagaga tcgtcctcac ctatcacgac gacgcggtga tactcggact
     2941 ggcgctcggt gccggcttct ggctcgtcct cctgtcggcg tcgatcgtcg cgctcgcgtt
     3001 cgaacggcgg aggccagggg gtgacggggt tagacggcct cgcccaccgc cgcccgacgc
     3061 ctgagctcgg cctcgatgaa tcgatcgatg tccccgccga gcacctgcga ggggtcgccc
     3121 atctcgaact cggtccggtg atccttcacc atctggtacg gggcgagcac gtaggagcgg
     3181 atctgcgagc cgaagtccac ggccttgcgc tcgcctcgca agacgtccat ctcttcgtcg
     3241 cgcttccggc gctccagctc cgcgaggcgg gctttcagga tccgcatcgc gacggcacgg
     3301 ttctggagct gcgacctctc gttctggcac gcgacgacga tccccgacgg caggtgtgtg
     3361 atccgcacgg cggaatccgt cgtattgacc gactgccctc ccgggccgga cgagcggaag
     3421 acgtcgatcc gcagatcctc gtcggggatc tctacctcct cctcctcggc ggcctcgagg
     3481 agcgggatca cgtcgagcgc cgcgaacgat gtgtgccggc gcttctgtga gtcgaacggg
     3541 ctgatccgga cgaggcggtg gacgccgcgc tcggccgaca ggagcccgta ggcgttcgtc
     3601 ccgtgcagca tgaacgtcgc ggacttgatc cccgcttcct ccccgtggtg gatctcgtcg
     3661 atctccagcc ggaagccaag cttctctgcc caccgtccgt acatccgcaa cagcatgtcg
     3721 gtccagtcct gggactccgt gccgcccgcg cccgcctgga gcgtggcgat cgcgtcgtgg
     3781 tcgtcgtacg gcccagagag gagcgccgtg agctccaccc cctgcagctc tcgctcgagc
     3841 cgttcgagcc cggcttcgag ctcactcatg agctcgtggt cggtctcgtc ctcgaggagt
     3901 tcgtcggtgg cttccaggtc gccgagggag cgctcgaggc gttcgtggcg gtcgagctga
     3961 tcacgcaggc gcgcgaggcg ctgggtcagc tgctgtccgc ggacagggtc gtcccagagc
     4021 gatggatcgg acgcgtcccg ctccacgtcg acgagctggg cacgcttgcc ttcgacgtca
     4081 aaggaactcc ttggcggcca ggatccggtc gcggagcttc ccgatccgtt cggtgtgatc
     4141 gctcatgtgc ccgattctag tggcggctcc cgatctcccc tccagtgacg agcgtcacgt
     4201 tctcgatgtg ctcggcacca ccgacgcgat cctcccaggt cggtaccgtg gcgccgaagg
     4261 accggacggg cgccccagcc gaagaccaga cgggcgcccc aaaggaccgg acgggcgccc
     4321 caaaggaccg gacgggcgtc tctagaggga cctctgagga ggacgacggt gagcacgagg
     4381 agccaagccc tcagcccagg gatctcgtgg aacgcaacgc gcgccgcgag cgtactcctg
     4441 gtcacgatca tcgcggtgat cgcgctgctg ttctggccgt ccatcgagtc cgctcgcccg
     4501 acggcccccg cggcgcggga gtcgaccggg ctcgcggccc cgcaccaacc gatcgtggtc
     4561 gacggcaggg tgtgcgtgca gtgcctgccg tgacctgaca ggagagaggt acccccctct
     4621 catcggcgac ggggcggcga caccggaacg acgtggcgaa cgtcactctc gatccgtgtg
     4681 gcccggcacc gaccccagga ggtgacgtgc ctagggtcgg tgcatcgacc agatcccgaa
     4741 gggatcgaga ggagaggggg agccatgagc gcccagggag caaccctcgg aagagggacc
     4801 gtccacgtcc cgatctggac cgtggccgtc gtcgttctcg ccgccgccac cgccgccctg
     4861 atcggattct ccacgctcga cacgagcgac acgatcgcgc cggctcggcc gctcgtgacg
     4921 gtgaccgatg cccagcggct cgcgaactcg accgccgcca tccgtgagca aggtgccgtg
     4981 gcccccgtcg ccgtcggcat ctcgcacgtg cccccggcgg ccgtcgtgcc ggtggggtac
     5041 gtcgggctcg agaacccggg ggcctacgtt ccccggtccg cgtacgccaa tggcctggag
     5101 aacccggggg cgtacgcgcc aagtgaaggc gcgcccgcgg tcacagctcc agcctcggcg
     5161 gggatcatgg tgaacggaga gatctgccac cagtgccggt gagcttcata actggagagc
     5221 cgaaggggcc ggcgcgaagc gccggcccct tcgtcatgtg gtcgttccga gcatcggatc
     5281 cgagggttcc tacgccacgg cgccgtggca cttcttgtac ttccggccgc tcccgcatgg
     5341 gcagggcgcg ttccggggca ccttgtcgct gatcacctgg ttcgggttgg gatcctgcgc
     5401 cccgctgggc cgggcggctt catcgtcccc atgacgctcg cgcactgcgg ccaccttctg
     5461 cgccgccgca cgtcgctgcg gctgctccgg ccggacgagc tccacgcggt agatgtagcg
     5521 gacgaactcc gcccggatcg tcgaggtgag ctcctcgaac atgtcgaagg cctcgcgccg
     5581 gtactccgtg atcggatcct tctgggcgta gctccgcaga tagatgcctt cctgcaggta
     5641 gtccatctcg tagaggtgct cgcgccagcg cgtatcgatg atgttgagca ggaccacccg
     5701 ctcgagctcc cgcatcatct ccacgccgag cgacgcctcc ttcgcggcgt aggcggtgag
     5761 cgcgtcctcc acgaacgctg cctggagcga ctccgtgtcg gcgatctcgt cgacctggtc
     5821 cttcgagagc gagaccggat agacctcggt gagggccgtg aggagaccct cgcgatccca
     5881 ttcctccgcg aagacatccg ccgggcaata gttggcgacg gtcgcctcga cgacgtcgcg
     5941 cacgaacccc agggcttcct cgctcagatc ctcgccctcg aggatcttcc ggcgctcccc
     6001 gtagatcacg gtgcgctggc cgttcatcac ctcgtcgtac ttcaggacgt tcttccggcg
     6061 ctcgtagttg agctcctcga tctgcccctg tgcgttctgg accgcgcggg tgaccatctt
     6121 cgcctcgatc ggctcgtcct cgggccactt cagccgttcc atgatcgagg agacccgatc
     6181 cgaggcgaac atccgcatca ggtcgtcctc cagcgagagg tagaacagcg acgccccggg
     6241 gtcgccttgg cggcccgagc gcccgcggag ctggttgtcg atccggcgag actcgtggcg
     6301 ctccgtgccg aggacgtaca gtccgccgag ctcgacgacc tcgtcgtgct ccgcgtcggt
     6361 ctgcgccctg aacttcgcga agatcggctc gtactccgcc tcgtacgcgg ctcgctcctc
     6421 ctcgtccatc tcgaacagga ggtaccggtc gttgtcgaag tcgcgggccg ccatctcctg
     6481 acgggcgagg aactcggggt tgccaccgag gaggatgtcc acgccgcgac cggccatgtt
     6541 cgtggcgacc gtgaccccgc ccttgcgccc
//
LOCUS       DASUYD010000239         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1068860, whole genome shotgun sequence.
ACCESSION   DASUYD010000239 DASUYD010000000
VERSION     DASUYD010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1068860"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF195_12610"
     CDS             <1..273
                     /locus_tag="VF195_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX6581698.1"
                     /translation="REIAVEFARRGVRCNALCPGPIDTPLLAELLADPARRQRRFVHI
                     PMGRLGQAEELAKAALFLASDDSSFMTGASLIVDGGITAAYVTPED"
     gene            306..587
                     /locus_tag="VF195_12615"
     CDS             306..587
                     /locus_tag="VF195_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581699.1"
                     /translation="MDATVATGVPVWPDVSHSSFDWLPLLLVLAALVLAPLVFGIRLS
                     AGSLICLSMGGTLVGWLGDSIGVVPAVAIGLIALAIASAWLRRTQTRRS"
     gene            665..2224
                     /gene="eat"
                     /locus_tag="VF195_12620"
     CDS             665..2224
                     /gene="eat"
                     /locus_tag="VF195_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine permease"
                     /protein_id="HEX6581700.1"
                     /translation="MAQERKVGSVTYQEVGQEYFQQRGLRRHAGVWSLWALGVAAVIS
                     GDFFGWNLGLLTGGFGGLAIATVFITIMYIGLCYSIAEMSPALPHTGGAYSFGRSAMG
                     PWGGFITGLAENMEYVVTPAVVVVAIGAYMNAITIELFDLDVPLQIWWLIAYVIFLAL
                     NIWGIELTMRFTVVICVLSIGILLFFFIAALTDFSVDHLTNIPPEGDNSKWLPFGIEG
                     IFKNLPFAIWFYLAIEELPLAAEESMDPRRDIPRATMWGLWTLVFLGVGTLILNTGTS
                     SGAAGIGATAEPLFQGFIDIFGDGTAAALLALIAVVGLVASFHTIIFAYGRNIFSLSR
                     AGYFPKWLSITHGTRKTPYVALLVGGVVGYGLAWWINESSGTNVGAALLTMAVFGAVI
                     SYFMQMLSFIMLRRKLPDIERPYRSPVGVWGAAIAGIIALVSLVSLYLDDSYRPGVIG
                     TAIWFVLGLLYFAIAGRNRLVLSPEEEFALTQGEAGIPGTQYATSREEQESILGGGSG
                     SAPPPPGTPPR"
BASE COUNT          371 a          799 c          832 g          462 t
ORIGIN      
        1 cgcgagatcg cggtcgagtt cgcgcgccgg ggcgtccggt gcaacgcgct ttgtccgggg
       61 ccgatcgaca ccccgctgct cgccgagctg ctcgccgatc ccgcgcggcg ccaacgccga
      121 ttcgtccaca tcccgatggg gcggctgggg caggcggagg agctcgcgaa ggcggccctg
      181 ttcctcgcga gcgacgactc gtcgttcatg acgggggcct cgctgatcgt ggacggcggg
      241 atcaccgccg cgtacgtgac gccggaggac taaaccgagg ccacctcggg aacgataggg
      301 agtccatgga tgcgacagtg gcgacgggcg tgcctgtttg gccggatgtg tcgcattcct
      361 ccttcgattg gctccccctg ctcctcgtcc tcgcggcgct cgtcctggcc ccgcttgtct
      421 tcggcatccg cctgtccgcg gggtcgctga tctgcctgtc catgggcgga acgctcgtcg
      481 ggtggctggg cgattcgatc ggggtcgtac ctgcggtcgc gatcggcctc atcgccctgg
      541 ccatagcctc ggcgtggttg cgcaggacgc aaacccgcag atcctgaacc tgtttgactc
      601 caccctcgac cgtgtggcct catacgcgca aggcctgtct gggcctgtcg gggagggagg
      661 agccttggcg caggaacgca aggtcggttc cgtcacctac caggaggtag ggcaggagta
      721 cttccagcag cgaggcctca ggcgccatgc cggcgtctgg tcgctctggg ccctcggggt
      781 cgccgccgtc atctccggtg acttcttcgg ctggaacctc ggtctgttga ccggtgggtt
      841 cggcgggctc gcgatcgcga cggtgttcat cacgatcatg tacatcgggc tctgctacag
      901 catcgcggag atgtcaccgg cgctaccaca caccggaggc gcctactcgt tcggacgatc
      961 cgcgatggga ccttggggcg ggttcatcac cgggctcgcc gagaacatgg aatacgtcgt
     1021 gacgccagcg gtggtcgtcg tcgcgatcgg cgcctacatg aacgccatca cgatcgagct
     1081 gttcgacctc gacgtgcccc tgcagatctg gtggttgatc gcgtacgtga tcttcctcgc
     1141 gttgaacatc tgggggatcg agctcacgat gcggttcacg gtcgtgatct gcgtgttgtc
     1201 gatcggcatc ctgctgttct tcttcatcgc ggcgctgacc gacttcagcg tcgaccactt
     1261 gaccaacatc ccacccgaag gcgacaactc caagtggctg ccgttcggga tcgaggggat
     1321 cttcaagaac ctcccgttcg cgatctggtt ctacctcgcg atcgaggagc tcccactcgc
     1381 ggccgaggaa tcgatggacc cgaggcggga catcccgagg gcgacgatgt ggggcctgtg
     1441 gacgctcgtg ttcctcgggg tgggaacgct gatcctcaac accggaacca gctcgggagc
     1501 cgcaggcatc ggcgcgacgg ccgagccgct gttccagggg ttcatcgaca tcttcgggga
     1561 cgggacggcg gcggcgctgc tggcactgat cgcggtcgtc ggcctggtcg ccagcttcca
     1621 cacgatcatc tttgcctacg gccgcaacat cttctccctg tcccgagccg ggtacttccc
     1681 gaagtggctc tcgatcaccc acgggacgcg caagaccccc tatgtcgccc tcctggtggg
     1741 cggcgtcgtg ggctacggcc tcgcgtggtg gatcaacgaa tcgtcgggga cgaacgtggg
     1801 agcggcgctg ttgacgatgg cggtcttcgg agccgtgatc tcgtacttca tgcagatgct
     1861 gtccttcatc atgctccgac ggaagctccc agacatcgaa cggccgtata ggagcccggt
     1921 tggagtctgg ggagcggcga tcgcgggcat catcgccctc gtctcgctcg tgtctctgta
     1981 tctggatgac agctaccgtc ccggggtgat cgggaccgcg atctggttcg ttctcggatt
     2041 gctgtacttc gcgatcgccg gccggaaccg ccttgtgctg tcgccggagg aggagttcgc
     2101 gctcacgcag ggtgaggcgg gtatcccggg cacgcagtac gcgaccagcc gagaagagca
     2161 ggagtccatc ctcggaggcg ggtcaggctc ggctccgcca ccacccggga ccccaccccg
     2221 gtagcggcga cctgagtccc atcgagcggc gggctcgcgc tgcgggcccg ccgttcgcgt
     2281 ttgacagccc gcaaccacgg atcttcgata gcggctccga ccgaaaggcg gacgaacgtg
     2341 acaggagcga ccggcacgct gaacgcgaag cagctcgagg agctcgccga ggcgggtgag
     2401 gtggacaccg tcctgtgcat gttcaccgac ctgcagggcc gcttcatggg caagcgcgtg
     2461 gtcc
//
LOCUS       DASUYD010000240         5431 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_409393, whole genome shotgun sequence.
ACCESSION   DASUYD010000240 DASUYD010000000
VERSION     DASUYD010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 5431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5431
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409393"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1041)
                     /locus_tag="VF195_12625"
     CDS             complement(<1..1041)
                     /locus_tag="VF195_12625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX6581701.1"
                     /translation="MLGESLQTTLVDAVPLSDVEFVVVDLETTGGSPIDSRITEVGAV
                     KLRGGERTGVFETLVNPGVPIPRQITHLTGIDDLIVAGAPPIEWVLPSFAEFARGCVF
                     VAHNARFDFTFLNVALERLDYDALPPPPVCTARLARRVVWPDVPNVRLHTLAQYFRTA
                     VRPSHRALPDAEACSEVLHGLLELGGRLGIVTLGDLHGAVRARGRPNFGKIRLADHLP
                     NAPGVYLFLGRDGRVLYVGKSVDLRSRVKSYFYGDGRKKVEDLLAETVSVDGVACGSE
                     LESLVLEARLIRDHEPKYNRRGKTWRRYAYLRIDDTEAYPRIKVVRETKGTGAFLGPF
                     PSSHVARLAKEAL"
     gene            complement(1145..3121)
                     /locus_tag="VF195_12630"
     CDS             complement(1145..3121)
                     /locus_tag="VF195_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HEX6581702.1"
                     /translation="MAAIEGGTLEPAAVTKYVTPLLIPPVMPRAATIRMPGGKPADYY
                     EISMKQFSQQILPAGMPATTVWGYGAVRSASKRGLLIHNAPSLTIESKWNRPVRIKWI
                     NDLKDANGDFRPHLLPVDPTLHWANPPGGTAGRDSRPTFTSTPGPYRGPVPIVTHVHG
                     AVNVGDESDGYAEAWYLPAANNLPAGHATEGTWYDFFKGKAASKFGVQWGPGFAMFQY
                     PNNQRESTIWYHDHTLGMTRLNVYAGPAGFFLVRGGPEGEKAILDSRTNTTAVLPGPA
                     PKEGDKFPPNKTYYEIPIAIQDRSFNDDGSLFYPDSRAFFDQFTGPYIPDSNVSPIWN
                     PEFFGNMIMVNGNTWPFQTVEQRRYRLRFLNGCQSRFLILDFSDIPGVEAWQIGNEGG
                     FLAAPVNLTADHGNRLLMGLAERADMIVDFTNVPVGNHVLRNVGPDEPFGGGEPEGEP
                     PDGFDIADPASTGQVMQFRVVPSVAPDPTTPPRFLVLPAITPLPTPDRTRPLALIEMM
                     SNVHDGPSEARLGVVDADGNPVDLMWASPVTENPSVGETEAWEFYNFTADAHPMHIHE
                     VAFEVVNRQALVVNEDGETEPPARLIGDPRPPEAWESGFKDTVIAYPGEVTRLRMKFT
                     NSGQFVWHCHIVEHEDNEMMRPYRIGDVQVGQPE"
     gene            complement(3390..4136)
                     /locus_tag="VF195_12635"
     CDS             complement(3390..4136)
                     /locus_tag="VF195_12635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581703.1"
                     /translation="MSRLLASEFRRFRSRRLVKALVGLELLAIVATGVIVVLTQEYDL
                     LGLPDVLMGTSLVLVSVAWVLGASAIGADWHAGHVTTILTWEPRRGRVMFAKIAASLT
                     SVFVVSLVIQALLGAALAAAAAGAGSTASADAAWLTESAGVALRVALLSTIFAGFGFG
                     LASAGRNTAVALGVGFGYLVIVENLVRGLRPQWTPWLLTENAGLFIVDSPIDFPLLAR
                     STIGAGLYLAAVGAVLLLAASGLFRMRDVN"
     gene            complement(4133..5062)
                     /locus_tag="VF195_12640"
     CDS             complement(4133..5062)
                     /locus_tag="VF195_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013116394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581704.1"
                     /translation="MDSVIEVEGLRKEYRRLRGRRTVAVDGLDLAVPQGGVFGFLGPN
                     GAGKTTTIRCLLGLVRPTGGRCRLLGHEPAGLHRVIGRVGSIVESPTMYPRFSGRRNL
                     ELLGRISGIGRARVARALEEVGLAERADDQVKTYSLGMKQRLGIAAALLKDPEVLILD
                     EPANGLDPAGIVEVRELMRRLGRDGRTVFVSSHILGEVQQTADRVAILARGRSVAVGP
                     VHEVLSVGRAAGLLVRLDDVPAGLRILGEAGIEARLDDGMIKVKLPPKEAARITRTLA
                     DRGLYLTELRADEADLETVFLELTGGASTEGAR"
     gene            complement(5148..>5431)
                     /locus_tag="VF195_12645"
     CDS             complement(5148..>5431)
                     /locus_tag="VF195_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013046542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX6581705.1"
                     /translation="FARQVDALGREGDVLVGISTSGNSENVVQAFERARAAGIRTIAL
                     TGVSGGKLGALADIEINVPSSETSHVQESHIAVGQLIAFLVEDELYPRP"
BASE COUNT          858 a         1856 c         1899 g          818 t
ORIGIN      
        1 gagcgcctcc ttcgccaggc gcgcgacgtg cgaggacgga aaggggccga ggaacgcgcc
       61 ggtccccttg gtctcgcgga cgaccttgat ccgcgggtac gcctcggtgt cgtcgatacg
      121 caggtaggcg tacctgcgcc aggtcttgcc gcggcggttg tacttgggct cgtggtcgcg
      181 gatcagccgg gcctcgagga ccagggactc gagctccgag ccgcacgcga cgccgtcgac
      241 cgagacggtc tccgcgagca ggtcctcgac cttctttcgg ccgtcgccgt agaagtagct
      301 cttcacccgc gaacgcaggt cgaccgactt gccgacgtac agcacgcggc cgtcgcgacc
      361 caggaacagg tagacgcccg gcgcgttggg caggtggtcg gccagccgga tcttcccgaa
      421 gttcggacgc ccgcgagcgc ggacggcgcc gtgcaggtcg cccagcgtaa cgatcccgag
      481 ccgcccgccg agctcgagca ggccgtggag cacctcggag caggcctccg cgtcgggcaa
      541 ggcccggtgc gacggtcgga ccgccgtgcg gaagtactgc gcgagcgtgt ggagccgcac
      601 gttcgggacg tccggccaca ccacgcgtcg cgcgagccgc gcggtgcaca cgggcggcgg
      661 cgggagcgcg tcgtagtcga gccgttcgag ggcgacgttc aggaacgtga agtcgaaccg
      721 ggcattgtgg gccacgaaga cgcagccgcg cgcgaactcg gcgaacgacg gcagcaccca
      781 ctctatcggg ggcgcgccgg ccacgatcag gtcatcgatc ccggtgaggt gcgtgatctg
      841 gcgggggatc ggcaccccgg ggttcacgag ggtttcgaac acgcctgtgc gctcgccgcc
      901 gcggagcttc acggccccga cctcggtgat ccgcgagtcg atgggggagc cgccggtggt
      961 ctcgaggtcg acgacgacga actcgacgtc ggacaaggga acagcgtcca cgagcgtggt
     1021 ctggagcgac tcgccgagca cccgcgcctc ccgcggggcc gcgcgaggac gagcaggcta
     1081 cccgaacagg tgttcgagcg tcaaggccca gaccgcagcc actcggcgga tgcggttgat
     1141 tcgtctactc gggttgcccc acctggacat ccccgatccg gtacggccgc atcatctcgt
     1201 tgtcctcgtg ctcgacgatg tggcagtgcc agacgaactg accggagttg gtgaacttca
     1261 tccgcaggcg ggtcacctcg cccgggtagg cgatcacggt gtccttgaag cccgattccc
     1321 aggcctccgg tggtcgcggg tcgccgatca gacgggccgg tggctcggtt tcgccgtcct
     1381 cgttgacgac caacgcttgc cggttcacca cctcgaacgc gacctcgtgg atgtgcatgg
     1441 ggtgagcgtc cgctgtgaag ttgtagaact cccaggcctc ggtctcgccg acgctcgggt
     1501 tctcggtgac cggactggcc cacatcaggt ccacggggtt gccgtcagca tccacgaccc
     1561 cgagccgggc ctccgagggc ccatcgtgaa cgttcgacat catctcgatc agggccagag
     1621 ggcgcgtcct gtcgggtgtc ggaagcggcg taatcgccgg gagcacgagg aaccgcggcg
     1681 gggtggtcgg atcgggcgct accgagggga ccacgcggaa ctgcatcacc tggccggtgc
     1741 ttgccgggtc ggcgatgtcg aacccgtccg ggggttcgcc ctcgggctcg ccgccgccga
     1801 acggttcgtc cgggccgacg ttgcgaagga cgtgattgcc gaccggcacg ttcgtgaaat
     1861 cgacgatcat gtcggcgcgc tccgccagac ccatcagcaa ccggttgccg tggtcggccg
     1921 tcaggttcac gggcgcggcc aggaagccgc cctcgttgcc gatctgccag gcctccacac
     1981 ccgggatgtc gctgaagtcg aggatcagga agcgggactg gcacccgttc aggaagcgaa
     2041 gtcgatagcg gcgctgctcc actgtctgga acggccaggt gttcccgttg accatgatca
     2101 tgttcccgaa gaactccggg ttccagatgg gggacacgtt gctgtccggg atgtacggcc
     2161 cggtgaactg gtcgaagaac gcccgtgagt ccgggtagaa cagcgagccg tcgtcgttga
     2221 acgaccggtc ctggatcgcg atcgggatct cgtagtaggt cttattgggg gggaacttgt
     2281 ccccctcctt cggtgcgggg ccgggaagga ccgccgtggt gttggtgcgg ctgtcgagga
     2341 tcgctttctc gccctcgggt ccgccgcgga ccaggaagaa gccggccggg cccgcgtaca
     2401 cgttgagacg ggtcatgccc agggtgtggt cgtgatacca gatcgtcgac tcgcgctggt
     2461 tgttcgggta ctggaacatc gcgaacccgg gaccccactg aacgccgaac ttcgaggcgg
     2521 ccttgccctt gaagaagtcg taccaggtgc cctcggtggc gtggcccgcc gggaggttgt
     2581 tcgccgcggg caggtaccag gcctcggcgt agccgtcact ctcgtcgccg acgttgaccg
     2641 cgccgtggac gtgggtcacg atcggcaccg ggcccctgta cgggccaggt gtgctggtga
     2701 acgtcggccg cgagtcgcgt ccggcagtgc cgccgggcgg gttggcccag tggagcgtcg
     2761 gatccaccgg aagcaggtgc gggcggaagt cgccgttcgc gtccttgagg tcgttgatcc
     2821 acttgatgcg caccggccgg ttccacttcg actcgatcgt gagcgacggc gcgttgtgga
     2881 tcagcaaccc ccgtttgctc gccgatctca cggctccgta tccccagacc gtcgtagccg
     2941 gcatccccgc ggggaggatc tgctgcgaga actgcttcat cgagatctcg tagtagtcgg
     3001 ccggcttccc gcccggcatc ctgatcgtcg cggcccgggg catcacgggg gggatcagga
     3061 gcggggtcac gtacttggtg acggccgcgg gctcgagcgt ccctccctcg atcgcggcca
     3121 tggcccgctg cacccatccg aagtgggtcg cggcgtacca cccgatcgtg gacgccccgg
     3181 tgagcttgaa gaaactccgt cgagtgagca tcatcatccc tcctgcgggc aacgctctcg
     3241 tcttagcgcg aggctcgctc acggtagatc gtcaggacag ggacttctag ccctgccaac
     3301 gtaggtcgtc tggaccacac ggcaaacacc accgagtctc agcgctggcg accagaacgc
     3361 ggtcggcggt cgtaggccca aggagtcggt cagttcacgt cgcgcatgcg gaacaggccg
     3421 gaggccgcga gcaggagcac ggcgcccacc gccgcgaggt acaggcccgc cccgatcgtc
     3481 gaccgtgcga gcagggggaa gtcgatcggc gagtccacga tgaacaggcc cgcgttctcg
     3541 gtgagcagcc agggcgtcca ctgcggccgg aggccccgca ccaggttctc gacgatcacg
     3601 aggtagccga agcccacccc gagcgcgacc gcggtgttgc gaccggccga tgccaggccg
     3661 aacccgaacc cggcgaagat cgtcgacagc agcgcgacac ggagcgcgac accggccgac
     3721 tccgtgagcc atgcggcgtc ggcgctggcg gtcgagcctg cgccggccgc ggcggccgcc
     3781 agcgccgcgc cgaggagcgc ctggatcacg agggacacga cgaacacgct cgtgaggctc
     3841 gcggcgatct tcgcgaacat cacccgtccc cgccgaggct cccaggtcag gatcgtggtg
     3901 acgtgccccg cgtgccagtc ggcgccgatc gcggaggcac ccaacaccca cgcgacggac
     3961 accagcacga gcgacgtgcc catcagcacg tcgggcagac ccaggaggtc gtactcctgc
     4021 gtgagcacca cgatcacgcc ggtggcgacg atcgcgagca gttcgagccc gacgagcgct
     4081 ttgacgaggc gccgggatcg gaagcggcgg aactccgagg cgagcagccg gctcatcggg
     4141 ccccctccgt cgaggcgcca ccggtgagct cgaggaagac ggtctcgagg tccgcctcgt
     4201 ccgctcggag ctccgtgagg tagagaccgc ggtcggcgag tgtccgcgtg atccgcgcag
     4261 cctccttggg aggcagtttg accttgatca tgccgtcgtc caaccgggcc tcgatcccgg
     4321 cctcaccgag gatccgcaga cctgccggaa cgtcgtcgag tcgcacgagg aggccggcgg
     4381 cccgaccgac cgacaggacc tcgtggaccg gaccgaccgc caccgacctt ccccgcgcca
     4441 agatcgcgac gcggtcggct gtctgctgga cctcgcccag gatgtggctc gagacgaaga
     4501 cggttctgcc gtcccggccg agccggcgca tgagctcgcg cacctcgacg atgccggcgg
     4561 ggtcgagccc gttcgccggc tcgtcgagga tcaggacctc gggatccttg aggagcgccg
     4621 ccgcgatccc cagacgttgc ttcatcccga gcgagtacgt cttgacctgg tcgtctgccc
     4681 gctccgcgag accgacctcc tccagggctc gggcgacgcg ggcgcggccg atgcccgaga
     4741 tcctcccgag cagctcgagg ttgcgccgcc ccgagaagcg tgggtacatc gtcgggctct
     4801 cgacgatcga tcccactcgt ccgatcaccc ggtgcagccc cgccggttcg tgccccagga
     4861 ggcggcaacg gcctccggtg ggccggacga gcccgaggag gcaacggatc gtggtggtct
     4921 tgccggcgcc gttcggtccc aggaacccga acacaccacc ctgcgggacg gcgagatcca
     4981 agccgtcgac cgcgacggtc ctgcggccgc gaagccgtcg gtactccttg cggagcccct
     5041 cgacctcgat gaccgagtcc atacggcaac cgtacgtcgc cctcggccgc gtgacatggg
     5101 cgcaggcacc acggtgcagc cggggtcagc accccgggcg cgccacgcta ggggcgtggg
     5161 tagagctcgt cctcgaccag gaacgcgatc agctggccga ccgcgatgtg cgactcctga
     5221 acgtgagagg tctccgagct cggcacgttg atctcgatgt cggcgagcgc cccgagcttg
     5281 cctcccgaca cgcccgtgag cgcgatcgtc cggatcccgg cggccctcgc ccgctcgaag
     5341 gcctggacga cgttctcgga gttcccggac gtcgagatgc cgacgagcac gtcgccctcg
     5401 cggcccagag cgtcaacctg gcgcgcgaac a
//
LOCUS       DASUYD010000241         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_628450, whole genome shotgun sequence.
ACCESSION   DASUYD010000241 DASUYD010000000
VERSION     DASUYD010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_628450"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..92)
                     /locus_tag="VF195_12650"
     CDS             complement(<1..92)
                     /locus_tag="VF195_12650"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX6581706.1"
                     /translation="MVVAHRGASVEQPENTIAAFEAAIDAGADVV"
     gene            complement(116..778)
                     /gene="nth"
                     /locus_tag="VF195_12655"
     CDS             complement(116..778)
                     /gene="nth"
                     /locus_tag="VF195_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012882091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6581707.1"
                     /translation="MAANEQLTRDDPIEERAPEIIRRLSEAYPDAKVALRFTNPLECL
                     VATVLSAQSTDETVNRVTETLFKKYPTAEDYLRVPEDELKADIHATGFFNQKAISIRE
                     ACRRIVEEYGGEVPGTMEDLITLRGVARKTANIVLGNSFGIVEGIAVDTHVNRLAHRL
                     GFSEQKNPDKIEQDLMRLVPKDQWFDVTYVLIDHGRALCTAKNPRCPECPVEPLCPAS
                     QA"
     gene            complement(789..1709)
                     /locus_tag="VF195_12660"
     CDS             complement(789..1709)
                     /locus_tag="VF195_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX6581708.1"
                     /translation="METVTVAAVQATPEFLDRDATVQKVVRLTKEAAGLGARLIVFPE
                     TFIPTYPDWVWRAAAWDGPFEALTARLREQSVEVPSSATDELGKAAKRAKSFLSVGVN
                     ELDGGTIYNAQLLFGPDGDLVRKHRKLMPTGGERLVWGFGDGSDLDPVETPFGRVGGL
                     ICWENLMPLARTAVYGMGVDIWTAPTWDNDENWIANLRHIAREGRVYVIGVCTLLRGS
                     DIPDDIPGRELWGGEEDWANPGWSAIVDPEGQLLAGPLVKEEGILTAEVDAEVARAAR
                     YKFDPVGHYSRPDVFTLVVNDAPKRAVTSE"
     gene            complement(1709..2320)
                     /locus_tag="VF195_12665"
     CDS             complement(1709..2320)
                     /locus_tag="VF195_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018150943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX6581709.1"
                     /translation="MEEMYHDGNRELQDRFDTRRLADRIEDVLVHDTFTEHDRAFIET
                     RDMFFLATADEDGKPNVSYKGGDPGFIRVVDEHTLAFPNYNGNGMYLSAGNLLRNPHV
                     GILFVDFERRNRMRVNGEASVDFDDELRSRWPEAQFVVRVRAREIFPNCPRYVHRYQK
                     VAASEFVPREDCETPVPSWKRADWAVDVLPDGDPALDPDATVV"
     gene            complement(2333..3046)
                     /locus_tag="VF195_12670"
     CDS             complement(2333..3046)
                     /locus_tag="VF195_12670"
                     /inference="COORDINATES: protein motif:HMM:NF015534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEX6581710.1"
                     /translation="MSWWSLLGSGEFQPWSEPVDRRLVEEADGDGRVLILPTASAPEG
                     KDVFERWGSLGLAHFSRLEIAAEVLELKTREDTDDPRLIERLEGASAIYLSGGNPAYL
                     ASTLTESDFLDTLVKQLDRGMAYAGCSAGVACLNEVTFDSDTTDVDQIFKPGLDLIPR
                     TLFAPHWDMVDTWMPGAAEFLVASAPEGSTFIGMDEDTAMVGDGSAWEVFGKSGIHVL
                     IDGTWTTYRDGDRFDLALI"
     gene            complement(3043..>3542)
                     /locus_tag="VF195_12675"
     CDS             complement(3043..>3542)
                     /locus_tag="VF195_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX6581711.1"
                     /translation="GIDLGRHSPNFSRGFWALKVWVSLLAHGRKAYGRRISHDAALAR
                     YLGARAEESDEFELMTPVGLSITCFRYVPTDLRSSGPGEESGGSGVLEREEYLDVLNN
                     RLMTEIQLDGRVYCSNAVLGERFCLRSCIVNFRTEAEDMDALLDVASELGAKLDVELR
                     PEALR"
BASE COUNT          550 a         1262 c         1147 g          583 t
ORIGIN      
        1 accacgtcgg cgccggcatc gatcgcggcc tcgaaagccg cgatcgtgtt ctcgggctgc
       61 tcgaccgacg cgcccctgtg cgccacgacc atcggccgag ccggccgttc gacgctcacg
      121 cctgactggc cgggcacagc ggctcgaccg ggcactcggg gcagcgggga ttcttcgcgg
      181 tgcagagcgc gcggccatgg tcgatcagca cgtacgtgac gtcgaaccac tggtccttcg
      241 ggaccaaccg catcaggtcc tgctcgatct tgtcggggtt cttctgctcg gagaacccca
      301 gccgatgggc aagccggttc acgtgggtgt ccaccgcgat cccctcgacg atcccgaacg
      361 agtttccgag gacgatgttc gccgtcttcc gggcgacgcc gcggagcgtg atcaggtcct
      421 ccatcgtgcc cgggacttcg ccgccgtact cctcgacgat ccgtcggcac gcctcgcgga
      481 tcgagatggc cttctggttg aagaacccgg tcgcgtggat gtcggctttc agctcgtcct
      541 cgggcacgcg gaggtagtcc tcggcggtcg ggtacttctt gaacagcgtc tccgtgaccc
      601 tgttcaccgt ctcgtcggtg gactgagccg acagcaccgt cgcgacgagg cactcgagcg
      661 gattcgtgaa gcgcagcgcg accttggcgt ccgggtacgc ctcggatagg cgtcggatga
      721 tctcgggagc tcgttcctcg atcgggtcat cgcgggtgag ctgctcgttc gcggccacgg
      781 cccggatgct actcggacgt caccgcccgc ttcggcgcgt cgttcaccac cagcgtgaac
      841 acgtcgggcc tgctgtagtg gccgaccgga tcgaacttgt accgcgccgc ccgagcgacc
      901 tcggcatcca cctcggccgt gaggatgccc tcttccttca cgagcgggcc cgccaggagc
      961 tgaccctcgg gatcgacgat cgcggaccag cccgggttcg cccagtcttc ctcgccgccc
     1021 cagagctctc gccccgggat gtcgtctggg atgtcggagc cgcggagcag cgtgcacacg
     1081 ccgatcacgt agacgcggcc ttcgcgggcg atgtgccgga ggttcgcgat ccagttctcg
     1141 tcgttgtccc acgtgggcgc ggtccagatg tcgacgccca tcccgtagac cgcggtgcgc
     1201 gcgagcggca tcaggttctc ccagcagatc agcccgccca cgcgcccgaa gggcgtctcg
     1261 acgggatcga ggtccgagcc gtcgccgaag ccccagacga gccgctcgcc gccggtcggc
     1321 atcagcttgc gatgcttccg cacgaggtcg ccgtcgggac cgaacagcag ctgcgcgttg
     1381 tagatcgtgc cgccgtcgag ctcgttcacg ccgacagaga ggaatgactt cgcgcgcttc
     1441 gccgccttcc ccagctcatc ggtcgccgac gacggcacct cgaccgactg ctcgcgcagg
     1501 cgcgccgtga gcgcctcgaa cgggccgtcc cacgcggccg cccgccacac ccaatccggg
     1561 taggtcggga tgaaggtttc cgggaacacg atcaggcgcg ctcccagacc ggcggcttcc
     1621 ttcgtcaggc ggacgacctt ctgcacggtc gcgtcccggt cgaggaactc cggcgtggcc
     1681 tgaacggcgg cgaccgtgac cgtctccatc agaccaccgt cgcatcggga tccaaggcgg
     1741 gatccccgtc gggcagcacg tcgaccgccc agtccgcgcg cttccaggag gggaccggcg
     1801 tctcgcaatc ttcccgcggt acgaactcgg acgcagcgac cttctgatac cggtgcacgt
     1861 accgcggaca gttcgggaag atctcgcgcg cccggacccg gacgacgaac tgcgcctcgg
     1921 gccaccggga ccgcagctcg tcgtcgaagt ccacggaggc ctcgccgttc acgcgcatcc
     1981 gattgcgcct ctcgaagtcg acgaacagga tcccgacgtg cgggttgcgc agcaggttcc
     2041 ccgccgacag gtacatcccg ttgccgttgt agttggggaa cgcgagcgtg tgctcgtcga
     2101 caacgcggat gaagcccggg tcgccgccct tgtagctcac gttcggcttg ccgtcctcat
     2161 cggccgtggc gaggaagaac atgtcccgcg tctcgatgaa cgcgcggtcg tgctccgtga
     2221 aggtgtcgtg gaccaacacg tcctcgatgc gatcggcgag ccggcgggtg tcgaagcgat
     2281 cctgcagctc ccgattcccg tcgtggtaca tctcctccat agctgccacc cctcagatca
     2341 gcgcgaggtc gaagcgatcc ccgtcccggt acgtggtcca tgtgccgtcg atcagcacat
     2401 ggatgccgct cttcccgaag acctcccacg ccgagccgtc gcccaccatc gccgtgtcct
     2461 cgtccatccc gatgaacgtg gaaccctctg gcgcggacgc cacgaggaac tcggctgctc
     2521 ccggcatcca tgtgtccacc atgtcccagt gcggtgcgaa gagcgtgcgc gggatcaggt
     2581 ccagtcccgg cttgaagatc tgatcgacgt cggtcgtgtc gctgtcgaac gtcacctcgt
     2641 tgaggcacgc gacaccggcg ctgcagcccg cgtacgccat cccccggtcg agctgcttca
     2701 cgagggtatc caggaagtcg ctctcggtga gcgtcgaagc gaggtacgcg gggttcccgc
     2761 cggagaggta gatggcggac gcgccctcga gccgttcgat cagccgcgga tcgtccgtgt
     2821 cttccctcgt cttgagctcc agcacctcgg cggcgatctc caggcgcgag aagtgggcga
     2881 ggcccaggga tccccaacgc tcgaagacgt ccttcccctc gggggcggac gcggtgggga
     2941 ggatcagcac ccggccgtca ccgtccgcct cctcgaccag ccggcggtcg accggctccg
     3001 accagggttg gaactccccc gagccgagca gcgaccacca gctcaacgca aggcctctgg
     3061 tcggagctcg acgtcgagct tcgcgccgag ctccgatgcc acatccagga gcgcgtccat
     3121 gtcctcggcc tccgtccgga agttcacgat gcacgaccgg aggcagaagc gctcgccgag
     3181 cacggcgttc gagcaataca cgcggccgtc gagctggatc tcggtcatca gccggttgtt
     3241 gaggacgtcc agatactcct cgcgctcgag gacgcccgaa cctccggact cctccccagg
     3301 accggatgaa cggaggtctg tcggcacgta gcggaagcac gtgatcgaga ggcccaccgg
     3361 cgtcatcagc tcgaactcgt ccgactcctc cgcccgggcg ccgaggtagc gagcgagcgc
     3421 ggcgtcgtgc gagatccggc gtccgtacgc cttccggccg tgcgcgagga gcgacaccca
     3481 taccttcagc gcccagaagc cacggctgaa gttcggcgag tgccggccga ggtcgatccc
     3541 cc
//
LOCUS       DASUYD010000242        13065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1070961, whole genome shotgun sequence.
ACCESSION   DASUYD010000242 DASUYD010000000
VERSION     DASUYD010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13065
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070961"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(575..3763)
                     /locus_tag="VF195_12680"
     CDS             complement(575..3763)
                     /locus_tag="VF195_12680"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX6581712.1"
                     /translation="MQVCPRCGDENSDRARFCQTCGGTLLAEPAGPREIRKTVTILFM
                     DVVGSTELGERLDPESTRRVMSRLFDTVKPVLERHGSTVEKFIGDAVLAVFGVPAVHE
                     DDARRACRAALEIQDEIGRLNKELERDWGMTIATRTGINTGEVLAGDPSSSQRLVTGD
                     PVNTAARLEQAAPPGAVLIGESTYRLVADAVEVEPVDPVVAKGKADAVAAYRLCSVVP
                     GTPVRVRRLDSPMVGRDRELRALLVAFERSLAESRFVLATVVGMPGVGKSRLVHEFVA
                     SIEDRATVLRGRCLAYGEGITFWPIAEVVRQAAAISEGASTEEARSRIEGLLPESEER
                     AVIQDRLAAAMGLGNAAGDIHETFWAIRRLFESLAVDRPLIVVVDDIHWAEPTLLDLI
                     EYLEGWSEDSPFFLLCLARPELLEVRQRWARAATRSVTIELDPLTGDQSDRLVQNLLG
                     SSSVSEEFRWRVVETAGGNPLFVEEMLGMLIDEEQLTHEGDRWLVRDELDTIAAPPSV
                     QSLLAARIERLPEEERRTLQHASVVGMVFGWDAVTVLSSPGDQPLVGSRLQALVRKGM
                     IRPEANFAGQDAFRFHHILLRDAAYRSLPRATRAGLHERFAEWMGEIVGDRVEEYEEI
                     LGYHLEQACRQQLAPTPGGDLASRASRLLASAGSRAFDRDDIAAALNLLSRALELDPT
                     DAPDALANRLLMAQALILSGDSGQANEMLSKLADRARAIGFLSLEWRAKIQQARLIAS
                     TTNITLDQITGTVDGAIETFTDLDDELGLSWCWSLLAWMHFNAGEASEAQKAASKSAS
                     HARSSGDVSAEMWSLVRALTCAVYGPTPVDEALTLSHDILDRVKGHPGHRAAVCQRVA
                     LLEAMLGNFEAARASITNARTLWQELGNIHGLAGMTNPASDVELYAGDVLAAERERRS
                     GYEAYRSMGAESYQATTAACLADLLVRLDRDDEALELAREGEKLTGADDITAQVPSRM
                     AIAKVLARRGAGLEAERLAREALDIAERTDWISLQGDTHMTLAEVLRHAGRRDEAAAS
                     AERAVERYERKGHVVGVGWARDLLREVASPSV"
     gene            complement(4034..4106)
                     /locus_tag="VF195_12685"
     tRNA            complement(4034..4106)
                     /locus_tag="VF195_12685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4071..4073),aa:Val,seq:tac)
     gene            complement(4097..4357)
                     /locus_tag="VF195_12690"
     CDS             complement(4097..4357)
                     /locus_tag="VF195_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581713.1"
                     /translation="MRPPDRPGCFLCHRPTFDPDKREVPWARAVAEGVQVLICPDCQH
                     DRADWQESLDRCPACGSTRLSVTLGDVVCRACGHADDAGAGA"
     gene            complement(4398..5153)
                     /locus_tag="VF195_12695"
     CDS             complement(4398..5153)
                     /locus_tag="VF195_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581714.1"
                     /translation="MADLRERLRGIEAAEPPDLWAGIESQAEEKGPAMDTNVTSIDAF
                     RTPGWQQRRRISAGLVAAAVVTLTVVVAWGALRVPTTVITPPQTLPTGWERCTNAALG
                     FSIGYPGDWHTTNVFDGTPDPANACQWFSPQPFGPQGNLVSEGWGYPLEVATRGPFEQ
                     VLAREIDPQLVDVLVNEEVLVDGRRAVRLEYETVSDEVGDNGLHYEYLIEVDPETTLI
                     VHTTTTRGIVGVYAENKVVVDRAIETIRFLPAA"
     gene            complement(5146..5607)
                     /locus_tag="VF195_12700"
     CDS             complement(5146..5607)
                     /locus_tag="VF195_12700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581715.1"
                     /translation="MVSARRDLEGLYREHGKRLWWALVAFSGDREIASDAESEAFAQA
                     LRRGDGIRDPLAWIWRAAFRIAAGELQERTRQAPPLTEPMYELPEPAAEVTAALRQLP
                     AQQRAAVVLHYYADRPIREIADVLGTSTSTVAVHLHRGRKRLRELLGEDDG"
     gene            5706..7349
                     /locus_tag="VF195_12705"
     CDS             5706..7349
                     /locus_tag="VF195_12705"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007267793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX6581716.1"
                     /translation="MPERAGTADLVITGADVYTMDAARRWAGAVAIRGDRIVAVGTEP
                     DVKDRFPGAKEVLHLPGRMVLPAFQDSHLHAPFAGRYRLHVSLHDLPGVDAYRDAVTA
                     YAEEHPDRPWIFGGGWLMAHFPGGTPTKDLLDDIVPDRPVFLLNRDVHGAWVNSKALE
                     IAHITRETPDPWDGRIERDPATGEPTGTLHEGAAYSFSDAHLPAPSQDEWERAILLAQ
                     EHLHSLGIAGWQDAWVTPATEVAYRSLAERGELTARVVGALWWDRHRGVEQVGELAAR
                     RDRGSVGTFHPTTVKIMCDGVLENYTGAMLRPYCDAAGHETDQTGITYLDRDQLVEAV
                     VALDARGFQVHMHAIGDRAVRNALDACEAARAANGARDSRHHIAHLQVIHPDDIHRFR
                     ELGVIANCQPYWAQHDSQMDELTIPFLGPDRAPRQYPFAALHAAGATLAFGSDWSVST
                     ANPLEEMEVAIRRADPSTREVPPFLPDQRLSLPVALAAFTKGASSINHDDDAGSIEEG
                     NRADLVVLDRNLFDLPDGTVAEARVEHTIAAGRVVSSPG"
     gene            7461..8552
                     /locus_tag="VF195_12710"
     CDS             7461..8552
                     /locus_tag="VF195_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017828883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX6581717.1"
                     /translation="MTKRFADVVAVDSLSLQVQPAEFLSLLGPSGCGKTTTLRMLAGF
                     EEPTAGEIFISGQAVQGTPPHKRNVNTVFQHFALFPHMSVAENVAYGLRQRNEEKSQI
                     ASKVGEALEMVKMSPLANRKPRQLSGGQQQRVALARALVNRPSLLLLDEPLGALDRKL
                     REEMQIELKLLQSQVGITFIFVTHDQGEALSMSDRIAVMLDGHVEQLADPDTIYDFPA
                     SAFVAGFIGQQNFFEGTAREQGSLVEDDGWTIRSSNQSPDAVDGRPALAAIRPEAVQL
                     LEEQPEDSVNSVPGTLAGISHLGDVIQFVVLTKGQKEILCRLPRPRAPKLEVGGDVWC
                     TWEPDLVHVFDAQQAAVVLADPAATGTDG"
     gene            8563..9747
                     /locus_tag="VF195_12715"
     CDS             8563..9747
                     /locus_tag="VF195_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009773699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX6581718.1"
                     /translation="MADELEGVRILASRATADRITRRHFLAGSAVAVFSSSVLLAACG
                     EDTPEPGGGGGTPSAGGEIEDRLNFFHWAEYDDPEVFKDFENQFGATTKIDVYASNEE
                     AIAKLTAASGTTGYDLVIPTGVYIPQMVELDLLEEINLDLIPNFENVDEIYRDQPWDP
                     GNKHSVCKDWGSTGWIYDKTKVSATIETWSDFIAVTQGEASGQTSVLDTPGNLAGIFF
                     WANGMNWATTDTAELDACEEFIVNEFAPHIKAYDSYPGIALTEGKYALSHAWNGDARQ
                     GLLSTDDPDRYVWGLGAPVTELWMDNWCIVKGAQNPNAAHAWINFICDPDVSLKDLAY
                     HGYNTGITGVEERAREEGLEYLDMIFFSDEEVATMDPGAVNEAQQRLVDIWNKAKAAS
                     GA"
     gene            9755..10696
                     /locus_tag="VF195_12720"
     CDS             9755..10696
                     /locus_tag="VF195_12720"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019179781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581719.1"
                     /translation="MSTAHRPVSVGEGAGRPRRRAPRFTLAIPAGLFFILFFAVPVLL
                     IVWYSFGYKPDIFRVIATDNPSFDRYVESFSSTFRDVFSGTLRISITGTILCLLIGFP
                     FAYWLAVKVPPRWRGLLLGLTIVPFWTNFLVRTLGWLILLQPNGALSNVLQDLQLIGG
                     PLDVLDTREAVQLGVVYNYLPLMIFPLFVALDRLDPALREASKDLGANRWKTFRQVTL
                     PLSMPGVVAGLLLVFIPLTGDYITAAVLGGAKGNMVGALVASQFNTAQNWALGSAMAV
                     ILIVMILASVAIFAVLGLFARALIRRARQVDLVVEPA"
     gene            10693..11598
                     /locus_tag="VF195_12725"
     CDS             10693..11598
                     /locus_tag="VF195_12725"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018155307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX6581720.1"
                     /translation="MSATTTVLRSKRDVTNGLLSVWGVIVFVFLFLPILMIVIYSFND
                     GRALVVWKEFGFRPYTDALNNPTIRGAIFTSIRAAAGAAVIATIMGTLAGIALARRPG
                     KWTIGFLALVFLVLVTPEIVDAIALLIWYVKLDDLVGGPFSPDFFFNFGLIRLFIGHA
                     IFASAVVTLIVRARLAGLDESLEEASADLYASPAQTFRKITLPLMMPAVLAGGLLAFS
                     LSLDNTIISSFVSTADASPWPVYVFSSLRSILRPSIAAMSTLVLLLTLFALALVAVVL
                     RRGGASGEEAAAMIVGVEATPGSAN"
     gene            complement(11613..12581)
                     /locus_tag="VF195_12730"
     CDS             complement(11613..12581)
                     /locus_tag="VF195_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX6581721.1"
                     /translation="MRARRLSFSRLFLVVISIVGLGIVGLSPSDALAQDTPSPGIAAE
                     DQIVFSGTVTVPQGERVGEVVVFSGRVHVHGVVTGDVVVLEGPVTVTGQVNGSVIAAD
                     GVIRLAESARVGGDVLAGEPVQIRPGAKVAGDTRGETRFSLEAPLAVLGELLGPIAIS
                     ISVLILGLVLVLLAPRGADAVAGALGAAPLASLGWGLLVVLSVPVTAVALVVSVLGVP
                     LGLALILSLGLWWLVGLTWAAWCAGRALVHAPGGRVQAFLAGWAILAAVGLVPILNAA
                     AWALAPVLGVGAMLVAAWRAREGRRHDGRHRRGGPRAEDEVEAGIA"
     gene            12641..>13065
                     /locus_tag="VF195_12735"
     CDS             12641..>13065
                     /locus_tag="VF195_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018815120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX6581722.1"
                     /translation="MGLEVFSPAVRDWFRTSFEAPTRAQRDGWKAIAAGDHALILAPT
                     GSGKTLAAFLWAIDRLSSEPPPEDPSARTRVLYVSPLRALAVDIEKNLRAPLAGIQHA
                     AERLGAPLAHAPVVAIRSGDTPARDRQRLIRRPPDVLITT"
BASE COUNT         2097 a         4594 c         4285 g         2089 t
ORIGIN      
        1 actagcttcc ggcaggttgt tcggtcacgg ttcgctcttg ccgtgggctg tgaggggagc
       61 accgcacgac cagcacaaag gcatccgcga tggagacgag ctgcgatggg tccggcgagc
      121 ctgatcgcga agcatgtgga agtcggcttc tttgtcgatg ccgtgtcacc ccatcaggtt
      181 cacttcgcta ttgctaagag cggcttgctt ccaggatgac gtcgatcgac ccagcctctg
      241 ggctgaccgc ttctcgtggg cgctgaggga ctcgaacccc cgtccttcgc cttgtaaggg
      301 cgaccgaaac atgcagctca gggctttgag caggcagttc gctctacttg tgactacgtg
      361 cgcgtacctt taggtgatcg tgcgtcgtgg tgcagaagtg gtgcacgaca ccctacgccc
      421 ggcccaacga aggtcggccc ggcctaggag tcgctggcgg ggagatcgtc ggcaaagaag
      481 tccggcgtca ctcgcggtaa ggcacgccac cagctcggag atcggaagtg cgtcgatcaa
      541 ctttctcgtg ggcgcgagcg accctggcga cgggctaaac gctcggcgag gccacctcac
      601 gcagcaggtc gcgggcccaa ccgactccca cgacgtggcc cttacgctcg taccgttcga
      661 ccgcccgctc ggctgaagcg gccgcctcgt ctcggcggcc agcgtgacga aggacctccg
      721 ccagcgtcat gtgcgtgtcg ccctgcaggg agatccagtc ggttcgctcg gcgatgtcca
      781 aggcctctct ggcgagtcgc tccgcctcca ggccagcccc acggcgagcg aggaccttcg
      841 cgatggccat gcgcgacggg acctgggcgg tgatgtcgtc cgcgccggtc agtttctcgc
      901 cctctcgggc caactcgaga gcctcgtcgt cgcggtcgag ccgaaccagc aggtccgcca
      961 ggcatgccgc cgtcgtcgcc tgatacgact ctgcccccat cgatcggtag gcctcataac
     1021 cggatcgccg ctcgcgctct gccgcaagca catccccggc gtacaactcg acgtcagagg
     1081 ctggattcgt catccccgcg agcccgtgga tgttcccaag ctcttgccac aacgtcctcg
     1141 cgttcgtgat cgaggctctc gccgcctcga agttgccaag catcgcttcg aggagggcca
     1201 ctctctggca cacagctgcc cgatgacccg ggtgcccctt gacgcgatcg agaatgtcgt
     1261 gtgacagggt caaagcctcg tccaccggag tcgggccgta tacagcacat gtgagagcgc
     1321 gcacgaggct ccacatctct gcagacacgt cacctgagga acgcgcatga gacgccgact
     1381 tcgaggccgc cttctgagct tcactcgcct ctccggcgtt gaagtgcatc caggcaagga
     1441 ggctccagca ccagctcagg cccaactcat cgtccagatc cgtgaaggtc tcgatcgctc
     1501 cgtctacggt ccccgtaatc tgatcgaggg tgatattcgt cgtggaggcg atgagccgag
     1561 cctgctggat cttcgcccgc cactcgagac tgagaaagcc aatggccctc gctcgatccg
     1621 ccagcttgct cagcatctcg ttcgcttgcc cggaatcgcc tgacaggatc agtgcttgcg
     1681 ccatcaggag gcggttggca agcgcatccg gagcgtccgt cgggtcgagt tccaatgcgc
     1741 gagacagaag gttgagggcg gctgcgatgt cgtcgcggtc gaaggcccga ctgccggccg
     1801 acgcgagcaa acgcgatgcc cgggacgcaa gatcgcctcc cggggtggga gcaagctgct
     1861 ggcgacacgc ctgctccaag tggtagccga ggatttcctc gtactcctcc actcggtctc
     1921 ccacgatctc ccccatccac tcagcgaacc gttcgtggag cccggcccgg gtcgctctcg
     1981 ggagcgatcg atacgcggcg tcgcggagaa ggatgtgatg gaagcggaag gcatcctgcc
     2041 cggcgaagtt cgcctccggc cggatcatcc ccttccgaac aagggcctgg agtcgggagc
     2101 cgacaagggg ttgatcgcca ggcgaggaaa ggaccgtgac cgcatcccac ccgaagacca
     2161 ttcccaccac cgaggcatgc tgcagggtcc gtcgctcctc ctccggaagc cgctcgatgc
     2221 gggcagccag gagactctgc acgctgggag gggccgcgat agtgtcgagt tcatccctga
     2281 caagccaacg gtcgccctca tgtgtcagct gttcctcgtc gatcagcatg ccgagcatct
     2341 cctccacaaa tagagggttg ccccccgcgg tctccacaac ccgccaccgg aactcttcgg
     2401 agaccgagct tgagccgagg aggttctgga caagacgatc gctctgatca ccggtgagag
     2461 ggtcgagctc gatcgtgacg gatcgcgtcg ccgcccttgc ccatctctga cgcacctcga
     2521 ggagctcggg tcgtgcgagg cagaggagga agaagggcga gtcctcgctc catccctcca
     2581 agtactcgat cagatcgagg agagtcggct cggcccaatg gatatcgtcg acgaccacga
     2641 tgagggggcg atcgactgcg agcgactcga acagcctgcg gatcgcccag aacgtctcgt
     2701 ggatgtcccc tgcggcgttt ccaagtccca tcgctgccgc caggcgatct tggatgaccg
     2761 cccgctcctc gctctcggga agaagcccct caatccggga cctcgcctcc tcggttgaag
     2821 cgccctccga aatcgcggcg gcctgacgaa ccacttcggc gatcggccag aacgtgatgc
     2881 cctccccgta cgcgaggcat cgcccgcgca acaccgtggc tcgatcctcg atcgaggcaa
     2941 cgaactcgtg gacgaggcgg gacttcccca cgcccggcat ccctacgacg gtggcgagaa
     3001 cgaaccgcga ttcggcgagc gagcgctcga acgccactag aagcgcgcgg agctctcgat
     3061 cccgccccac catgggcgaa tccagccgtc gaaccctcac cggggtcccc ggaacgacgg
     3121 agcagagccg gtacgcagcc acggcatcgg ccttgccctt ggccaccacg gggtcgacgg
     3181 gctcaacttc gacggcgtcg gcgaccaggc ggtaagtgga ttcccctatt agcaccgctc
     3241 cgggcggagc tgcctgctcc agccgagccg ctgtgttcac cggatcaccg gtcaccagcc
     3301 tctggctcga tgagggatcc cccgcaagca cctctccggt gttgatgccg gtacgggtcg
     3361 cgatcgtcat tccccagtcg cgctcgagct ccttgttcag ccggccgatc tcgtcctgga
     3421 tctcgagcgc cgcccgacat gcccgccggg catcgtcctc gtgcaccgcc gggacgccga
     3481 acaccgccag gacggcatcg ccgatgaact tctccaccgt gctgccgtga cgctccagca
     3541 caggcttgac tgtgtcgaag agacgactca tcactcgcct cgtggattcg gggtcgagtc
     3601 gctcgccaag ctcggtcgaa ccgaccacgt ccatgaacag gatcgtgacc gtcttgcgga
     3661 tctcccgcgg tccggcgggt tcggcaagga gggttccgcc acacgtttga cagaagcgag
     3721 cgcggtccga gttctcatct ccacacctcg gacacacctg catgatcgga gtctaggctc
     3781 gcgcccaagg cagagtaggg gtcgccgtgc tcgaccgact ctcacgtctc gggcctggag
     3841 ctcgcccgcc gaacgcaacc ccatgtggcg cttcgtccca ggcgagatgg acccgccggg
     3901 tggcctgctc ggctctcaca tcgcgtgaca gagtcgatac ttccgggcac caaagtggtg
     3961 cacgttcacc tgagaggact ttaggtagcg ggtgtgacac gtgaatgagg ctattgacct
     4021 gcacgtttac ttgtgggcgc tgagggactc gaacccccga ccttcgcctt gtaagggcga
     4081 cgctcttcca gctgagctaa gcgcccgctc ccgcatcatc ggcatgcccg caggctcggc
     4141 acaccacgtc ccccagggtg acggacaagc gggtcgaccc gcaagccggg caccggtcga
     4201 ggctctcctg ccagtcggcg cgatcgtgct ggcagtccgg gcagatcagg acctggactc
     4261 cctcggccac cgcgcgcgcc cacggcacct cgcgcttgtc gggatcgaag gtggggcggt
     4321 ggcagaggaa gcagcccggc cggtccggcg gcctcacgga gcgcaggata tccgttccgt
     4381 gaggccagcg gctggaacta ggcagcggga aggaagcgga tggtctcgat cgcgcggtcc
     4441 accacgacct tgttctccgc gtagacgccc acgatgcccc gcgtcgtcgt cgtgtgcacg
     4501 atgagcgtcg tctccggatc gacctcgatc aggtactcgt agtggagccc gttgtcgccg
     4561 acctcgtcgc tcacggtctc gtactcgagg cgcaccgcac ggcgcccgtc caccaggacc
     4621 tcttcgttca cgagcacgtc gacgagctga ggatcgatct cccgcgccag cacctgctcg
     4681 aacggacccc tggtcgcgac ctccagcggg tagccccagc cctcggacac gaggttcccc
     4741 tgtggaccga acggttgcgg ggagaaccac tggcaggcgt tcgccgggtc cggggtgccg
     4801 tcgaagacgt tcgtcgtgtg ccagtcacca gggtagccga tcgagaagcc gagcgccgcg
     4861 ttcgtgcacc gctcccaccc ggtcggaagc gtctgcggcg gtgtgatcac cgtcgtcgga
     4921 acccggagcg ccccccacgc caccacgacc gtcagggtga caaccgcggc cgcgacgagt
     4981 cccgccgaga tccggcgccg ctgctgccag ccgggcgtcc ggaacgcgtc gatcgaagtg
     5041 acgttcgtgt ccatcgcagg ccccttctct tccgcctgcg actcgatccc cgcccacagg
     5101 tcgggcggct cggccgcttc gatccctcgg agtcgttcgc gcagatcagc catcgtcttc
     5161 tcccagcagt tctcgaagtc gcttgcggcc gcggtgcaag tgcaccgcca ccgtggacgt
     5221 ggacgtcccg agcacgtcgg cgatctcgcg gatcggtcgg tccgcgtagt aatggaggac
     5281 gaccgcggcc cgctgctgcg cgggcagttg ccgaagtgcc gcggtgacct cggcggccgg
     5341 ctcgggcagc tcgtacatcg gctccgtcag cggcggcgcc tgcctcgttc gttcctggag
     5401 ctcaccggcc gcgatccgga acgccgccct ccagatccag gcgagggggt ccctgatgcc
     5461 gtccccccgc cggagggcct gtgcgaacgc ctcggactcc gcgtcgctgg cgatctcgcg
     5521 atcaccgctg aacgccacga gggcccacca gaggcgcttg ccgtgctccc ggtagagccc
     5581 ctccaggtcg cgtcgcgcgc tcaccatgac gtccatgccc ttcagggccg gttggatgcg
     5641 gtgacattta cacgccggcg gatccggtgc agcgaggcgc gggatgaccg ctatcctccc
     5701 gcccgatgcc ggagcgagcc gggaccgccg acctcgtgat caccggggcc gacgtctaca
     5761 cgatggacgc cgcgcgccgg tgggcgggcg ccgtggcgat ccgcggcgac aggatcgtcg
     5821 cggtcggcac ggagcctgac gtgaaggatc ggttccccgg cgcgaaggag gtgctccacc
     5881 tcccgggccg gatggtgctg ccggcattcc aggactccca cctccatgcg ccgttcgccg
     5941 gccggtaccg gctgcacgtg tccctgcacg accttccggg cgtggacgcc taccgggacg
     6001 cggtcacggc gtacgccgag gagcaccccg accggccgtg gatcttcggc ggtggctggc
     6061 tgatggcgca cttccccggc ggcacgccga cgaaggatct cctcgacgac atcgtgccgg
     6121 accggccggt cttcctgctg aaccgcgacg tccacggggc gtgggtgaac tcgaaggcgt
     6181 tggagatcgc gcacatcacg cgcgagacgc ccgacccctg ggacgggcgg atcgagcgtg
     6241 atcccgcgac cggcgagccc accgggaccc tgcacgaggg cgccgcctac tcgttctcgg
     6301 acgcccacct gccggcgccg tcacaggatg agtgggaacg ggcgatcctc ctcgcgcagg
     6361 agcacctaca cagcctcggt atcgcggggt ggcaggacgc ctgggtcacg ccggccacgg
     6421 aggtggcgta ccgctcgctc gccgagcgcg gtgagctcac cgcccgggtg gtcggggcgc
     6481 tctggtggga tcggcatcgc ggggttgagc aggtcggtga gctcgccgcg cggcgtgacc
     6541 gcggttcggt ggggaccttc caccccacca ccgtgaagat catgtgcgac ggcgtgctcg
     6601 agaactacac gggggccatg ctccgcccct actgcgacgc cgccggccac gagacggacc
     6661 agacgggcat cacctacctg gatcgcgacc aactggtcga ggccgtcgtg gccctcgatg
     6721 cgcgtgggtt ccaggtccac atgcacgcga tcggcgaccg ggcggtccgc aacgctctgg
     6781 acgcgtgcga ggcggcgcgc gccgccaacg gcgcccgcga ctcccggcac cacatcgccc
     6841 atctgcaggt gatccacccc gacgacatcc atcggttccg ggagctcgga gtgatcgcga
     6901 actgccagcc gtactgggcc cagcacgact cacagatgga cgagctcacg atcccgttcc
     6961 tgggaccgga ccgagcgccg cgccaatacc cgttcgcggc gctgcatgcc gcgggagcca
     7021 cgctggcgtt cggctccgac tggtcggtct ccacggccaa cccgctggag gagatggagg
     7081 tggcgatccg ccgggccgac ccctccacga gggaggtccc gccgttcctt cccgaccagc
     7141 gcctgtccct ccccgtggcc ctcgccgcgt tcacgaaggg tgcatcctcc atcaaccacg
     7201 acgacgacgc cggctcgatc gaagagggga atcgggccga cctggtcgtg ctcgaccgga
     7261 acctcttcga cctccccgac ggcaccgtgg ccgaggctag ggtggagcac acgatcgccg
     7321 ccggccgggt ggtctcgtcc cccggctgag ccgttggttg cgcgaaccgg gctcgttcgt
     7381 cagactccca cggttgggcc gacccgaggg gggtgccgca gggcgtgctg agcgaagagg
     7441 gttcagtcga gctggtcgac gtgacgaagc gcttcgcgga cgtcgtcgcc gtcgattccc
     7501 tctcgctcca ggtgcagccg gctgagttcc tctccctcct cggtccctcg ggctgcggga
     7561 agaccacgac cttgcggatg ctggccggct tcgaggagcc gacggcgggt gagatcttca
     7621 tctcgggtca ggccgtgcaa ggcacgccgc cgcacaagcg gaacgtgaac accgtcttcc
     7681 agcacttcgc cttgttcccg catatgagcg tggcggagaa cgtcgcgtac ggcctccggc
     7741 agaggaacga ggagaagtcc cagatcgcga gcaaggtcgg tgaggcgctc gagatggtga
     7801 agatgtcgcc gctggcgaac cggaagccgc gccagctctc cggcggccag cagcagcgcg
     7861 tcgcgctcgc ccgcgcgctc gtgaaccgac ccagcttgct cctgctggac gaaccgctcg
     7921 gcgcgctcga ccggaagctc cgggaggaga tgcagatcga gctgaagctc ctgcagtcgc
     7981 aggtcggcat cacgttcatc ttcgtgaccc acgaccaggg cgaagcactg tcgatgtcgg
     8041 accgcatcgc cgtgatgctg gacggccacg tggagcagct cgccgaccct gacacgatct
     8101 acgatttccc ggcctcggcc ttcgtggccg ggttcatcgg gcagcagaac ttcttcgagg
     8161 gaacggcccg cgagcagggc agtctcgtag aagatgatgg atggacgatc cgctcgtcga
     8221 accagtcccc ggacgcggtc gacggtcggc ccgccctggc cgcgatccgg ccggaggcgg
     8281 tacagctcct ggaggagcaa ccggaggact cggtgaactc ggtccccggg acgctggccg
     8341 gcatctcgca cctcggtgac gtgatccagt tcgtcgtgct cacgaagggg cagaaggaga
     8401 tcctctgccg gcttccccga ccgagggccc cgaagctcga ggtgggaggc gacgtctggt
     8461 gcacgtggga gcccgacctg gtccacgtgt tcgacgcgca acaggcggcg gtggtcctgg
     8521 cggacccggc cgcgaccgga acggacggat aggaggcccc agatggcaga tgagttggaa
     8581 ggcgtgagga tcctcgcctc gcgggcgacc gcggaccgga tcacgcgacg gcacttcctc
     8641 gcaggctcgg cagtggcggt gttctcgagc agcgtgctgc tcgcggcctg cggcgaagac
     8701 acgccggagc ccgggggcgg cggcggaacc ccgtcggccg gcggggagat agaggaccgg
     8761 ctgaacttct tccactgggc ggagtacgac gacccggagg tcttcaagga cttcgagaac
     8821 cagttcggtg ccaccacgaa gatcgacgtg tatgcctcga acgaggaggc gatcgccaag
     8881 ctcacggcgg cctccggaac caccggctac gatctcgtga tcccgaccgg tgtgtacatc
     8941 ccgcagatgg tcgagctcga tctcctcgag gagatcaacc tcgacctgat cccgaacttc
     9001 gagaacgtcg atgagatcta tcgcgaccag ccatgggacc cggggaacaa gcactcggtc
     9061 tgcaaggact ggggctccac cgggtggatc tacgacaaga cgaaagtgtc cgccacgatc
     9121 gagacctgga gcgacttcat cgccgtcacc cagggcgagg cgagcggcca gacgtccgtc
     9181 ctggacacgc ccggcaacct ggctggcatc ttcttctggg cgaacgggat gaactgggcc
     9241 acgacggaca ccgccgagct cgacgcctgc gaggagttca tcgtgaacga gttcgcgccg
     9301 cacatcaagg cgtacgactc gtacccgggt atcgcgctca cggagggcaa gtacgcgctc
     9361 tcccacgcgt ggaacggtga cgcccgccag ggcctgcttt cgacggacga ccccgaccgg
     9421 tacgtgtggg gcctcggagc gccggtgacc gagctctgga tggacaactg gtgcatcgtg
     9481 aagggcgcgc agaaccccaa cgccgcccac gcatggatca acttcatctg cgatccggac
     9541 gtctcactga aggacctcgc ctaccacggc tacaacaccg gcatcaccgg ggtcgaggag
     9601 cgggcccggg aagagggcct cgagtacctc gacatgatct tcttctccga cgaggaggtc
     9661 gccacgatgg acccgggggc cgtgaacgag gcccagcagc gcctggtcga catctggaac
     9721 aaggcgaagg ccgcctcggg agcctgatcc cgaggtgtcg acggcgcatc ggccggtcag
     9781 cgtcggcgag ggcgcggggc gcccgcgccg acgcgcgccc cggttcaccc tcgcgatccc
     9841 cgccggcctg ttcttcatcc tgttcttcgc ggtcccggtc ctgctgatcg tgtggtactc
     9901 gttcggctac aaaccggaca tcttccgcgt gatcgcgacg gacaacccgt cgttcgatcg
     9961 ctacgtcgag tcgttctcct ccacgttccg cgacgtgttc agcggcacgc tgcggatctc
    10021 gatcacgggc acgatcctgt gcctgctgat cgggttcccg ttcgcgtact ggctcgcggt
    10081 caaggtgccg ccacgctggc gagggttgct gctcggcctg acgatcgtgc cgttctggac
    10141 gaacttcctc gtgcgcacgc tcggctggtt gatcctgctc caaccgaacg gagcgttgtc
    10201 gaacgtcctc caggacctcc aactgatcgg tgggccactg gatgtcctcg acacgcgcga
    10261 ggccgtccag ctcggcgtgg tctacaacta cctcccgctc atgatcttcc cgctcttcgt
    10321 cgcgctcgat cgcctcgatc cggcgctccg tgaggcctcg aaggatctcg gcgcgaaccg
    10381 ctggaagact ttccgccagg tcacgctgcc tttgtcgatg cccggcgtcg tcgccgggct
    10441 cctgctcgtc ttcatccccc tcacgggcga ctacatcacc gcggccgtgc tcggtggagc
    10501 gaagggcaac atggtgggcg cgctcgtggc cagccagttc aacaccgcac agaactgggc
    10561 cctcggatcg gcgatggccg tgatcctgat cgtgatgatc ctcgcgtcgg tggcgatctt
    10621 cgcggtcctg ggattgttcg cacgcgcgct gatccggcgc gcgcgacagg tcgatctggt
    10681 cgtggagccg gcgtgagcgc gacgacgacc gtcctccgtt cgaagcgcga cgtcacgaac
    10741 gggctcctct ccgtgtgggg tgtcatcgtc ttcgtcttcc tcttcctgcc gatcctcatg
    10801 atcgtgatct actcgttcaa cgacggccgt gcactcgtgg tgtggaagga gttcggattc
    10861 aggccgtaca cggacgccct gaacaacccc acgatccgtg gcgcgatctt cacgtcgatc
    10921 cgggccgccg ccggagccgc ggtgatcgcc acgatcatgg gtacgctcgc cgggatcgcg
    10981 ctcgcgcgcc gcccgggcaa gtggacgatc ggcttcctcg ctctcgtgtt cctcgtgctc
    11041 gtgacgcccg agatcgtcga cgcgatcgcg ctcctgatct ggtacgtgaa gctcgacgat
    11101 ctcgtcggcg ggccgttctc gcccgacttc ttcttcaact tcggactgat ccggctcttc
    11161 atcgggcacg cgatcttcgc gtcggcggtg gtgaccctga tcgtgcgggc gcggctcgcc
    11221 ggcctcgacg agtcgctgga ggaagcctcg gccgacctct acgcctcgcc cgcacagacg
    11281 ttccgcaaga tcacgctccc gctgatgatg cccgccgtgc tcgcaggcgg actgctcgcc
    11341 ttcagcttga gcctggacaa cacgatcatc tcgtcgttcg tctcgaccgc cgacgcctcg
    11401 ccgtggccgg tctacgtgtt cagttcgctg cggagcatcc tccggccgtc gatcgcggcg
    11461 atgtccacgc tcgtgctcct gctgaccctg ttcgccctgg cactcgtggc cgtcgtgctc
    11521 cgccgaggcg gcgcatcagg cgaggaggcg gcggcgatga tcgtgggtgt ggaggccacc
    11581 cccggctcgg cgaactgagg cctcgaccgc cctcacgcga tcccggcttc gacctcgtcc
    11641 tccgctcgtg ggccgcctcg ccgatgacgc ccgtcgtgtc ggcggccctc ccgagctcgc
    11701 cacgcggcaa ccagcatcgc ccccacgccg aggaccggcg cgagtgccca agccgccgcg
    11761 ttcaggatcg gcaccagacc gacggcggcg aggatcgccc agccggcgag gaacgcctgc
    11821 acacggccgc ccggcgcgtg gacgagggcg cggcccgcgc accaggccgc ccaggtgagc
    11881 ccgaccaacc accagaggcc gagggacagg atcagcgcga ggccgagcgg cacgccgagc
    11941 accgagacca cgagtgccac ggccgtgacg gggacgctga ggaccacgag gagcccccat
    12001 ccgagcgagg cgagcggtgc cgcgccgagc gcgccagcga ccgcgtcggc gccgcgcggc
    12061 gcgagcagga cgagcacgag gcccaggatg agcaccgaga tcgagatcgc gatgggcccc
    12121 aggagctccc cgagcacggc gaggggtgcc tccaaggaga accgcgtctc accccgcgtg
    12181 tcccccgcga ccttcgcgcc cggccggatc tggaccggct cgccggccag gacatcgccg
    12241 ccgacgcgcg cgctctccgc gaggcggatc accccgtcgg cggcgatcac cgacccgttc
    12301 acctggcccg tcaccgtgac cggtccttcg aggaccacca cgtcgccggt caccacgccg
    12361 tgcacgtgca cccggcccga gaagaccacc acctcaccca cccgctctcc ctgtggcacg
    12421 gtgaccgtcc cgctgaacac gatctgatct tctgctgcga tccccggcga gggtgtgtcc
    12481 tgcgcgaggg cgtccgaggg cgacaagccg acgatgccga ggccgacgat gctgatgacc
    12541 acgaggaaga gccgggagaa cgagaggcgg cgggcacgca tgcgcgggca tcctaccggc
    12601 gccttcccgc gtgtcgggaa cacccgatag gttgtggcac gtgggcctcg aggtcttctc
    12661 tcccgccgtc cgcgactggt tccggacctc gttcgaggcg cccacgcgcg cccagcgcga
    12721 cgggtggaag gcgatcgccg ccggcgacca cgcgctgatc ctcgccccca ccgggtcggg
    12781 gaagacgctc gccgcgttcc tgtgggcgat cgaccggctc tcctccgagc cgccgcccga
    12841 ggatccctca gcgaggacac gggtgctcta cgtgtcgccg ctgcgagcgc tcgcggtcga
    12901 catcgagaag aacctccgcg ccccgctcgc cgggatccag cacgcggccg agcggctcgg
    12961 ggcaccgctt gcccacgcgc cggtggtcgc gatccgaagc ggcgacacgc ccgcgcgcga
    13021 tcggcagcgc ctgatccggc gcccgcccga cgtcttgatc accac
//
LOCUS       DASUYD010000243        12287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1953531, whole genome shotgun sequence.
ACCESSION   DASUYD010000243 DASUYD010000000
VERSION     DASUYD010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12287
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1953531"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1236)
                     /gene="dnaJ"
                     /locus_tag="VF195_12740"
     CDS             complement(79..1236)
                     /gene="dnaJ"
                     /locus_tag="VF195_12740"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012950765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX6581723.1"
                     /translation="MADEVRREWFDKDYYAVLGVPKNASAAEVKKAYRTLAQQHHPDT
                     KQGDPDAENRFKEISAAYDVLGDAEKRKSYDQVRDMSASGFGPGFGRPGAGAPGAAGA
                     GWPGGVRYEQVNVDDLGDLFGGLFGGAGRRSGRRPAAQRGADLETSVSISFEDAVAGT
                     TLPIKIDGPAVCARCHGSGAEPGTQPITCPQCAGSGEIAENQGFFSMSRPCPRCGGAG
                     RIVESPCTKCGGSGAERRTRTLHVKIPAGVRDGARIRLAGKGEPGPAGGRPGDLFVKV
                     AVESHPVFGRKGDDLTLDLPISYTEATLGANVEVPTLNGPVTLKVPAGTPSGKTFRVK
                     GRGAPRKGGRGDLLAKVLVDVPEKVSREEKELLRQLHEASKGSPRKRLGVR"
     gene            complement(1240..1803)
                     /locus_tag="VF195_12745"
     CDS             complement(1240..1803)
                     /locus_tag="VF195_12745"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX6581724.1"
                     /translation="MTEDRPDQGSVEEAAAREAAASEARPEAAASDAGPDVGTADVQT
                     ALAEADQYREHLQRLQAEFDNYRKRVLREQTSAVELAAQPVLRRLLEVLDDFDLALMH
                     AEDRPDFDRFLHGVELVYAKLIDTLRAEGLERMEAQGKPFDPELHEALMQSGEGDGDP
                     VVEDVLRPGYTLKGRVLRPAGVRVERK"
     gene            complement(1800..2216)
                     /locus_tag="VF195_12750"
     CDS             complement(1800..2216)
                     /locus_tag="VF195_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX6581725.1"
                     /translation="MLAKWDLFRDMRSVEDEFDRVAGRAFSRDTWVPALDVRESQDRF
                     DVTVDLPGLDPDDVNVTFEDGMLSITGKREFSAEDRGETWHRIERSFGTFARSMRLPQ
                     TADTERIEATFDKGVLTVSVPKTEQAKPRTIEVRSR"
     gene            complement(2216..4090)
                     /gene="dnaK"
                     /locus_tag="VF195_12755"
     CDS             complement(2216..4090)
                     /gene="dnaK"
                     /locus_tag="VF195_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012850627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX6581726.1"
                     /translation="MARAVGVDLGTTNSVVAVLEGGEPVVVPNAEGSRTTPSVVGFSK
                     TGEILVGEVAKRQAITNPDRTIRSVKRHMGAKDWSVDVDGKGWTPQEISAQILNKLKR
                     DAEAYLGDTVTQAVITVPAYFDDAQRQATKEAGQIAGLEVLRIINEPTAAALAYGLDK
                     QQTEQTILVFDLGGGTFDVSLLEIGEGVVEVKATHGDTQLGGDDWDQRVIDWLADRFK
                     GNYSIDLAKDRMAMQRLKEAAEKAKIELSQVTETTINLPFISAGPDGPLHLEEKLSRT
                     EFERMTADLVDRCKGPFEQAIADWGKDAGAIDHVILVGGSTRMPMIQELVKDLTGGKD
                     PHKGVNPDEVVAVGAAIQAGVLKGDVKDILLLDVTPLTLGVETKGGLFTKLIERNTTI
                     PTRKSEIFTTADDNQTTVEIHVLQGEAETVMSPGVKSLGRFHLTGIPPAPRGVPQIEV
                     AFDIDANGIVNVSAKDLASGKEQAMTITGGTALSKEEIDRMMKEAEGFAAEDHTRREA
                     AEARNQADQLTYQVDSFLRDNAENISDADKAELTSKNDELKQALKDESAETESLRTAS
                     DALMQVWQRVGQSMHEQAAQAQAAAGSGGDETSASAGEQPDDDEDVVEGEIVDEGGAS
                     "
     gene            4371..5060
                     /locus_tag="VF195_12760"
     CDS             4371..5060
                     /locus_tag="VF195_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581727.1"
                     /translation="MATSSTTLRTRRGVHPTVAHTVDTLLLQERTDWAIHIHDGLTQS
                     VTSAILELQGLQHRIRTEPELVCSSLKEIEDAIRNDLKQIREILFELDGGTPHREPPL
                     ATFVSELVERWHLSARVSIEGEVERIPDVTLETANAIVAEALANAAKHSGAPEVSVRI
                     IAGPEELRIEVEDRGSGVVNPTDEEMHFGLRLMRSRAERIGGTIEIDSKPGSGTRVVA
                     VLPVGRRGDEG"
     gene            5057..5731
                     /locus_tag="VF195_12765"
     CDS             5057..5731
                     /locus_tag="VF195_12765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581728.1"
                     /translation="MMKILIVDDHALVRRGLHYVVKEGFPDAEVTEAESAAAALEHIH
                     NGKEIDLALVDVRMPDLDGLELLRALKTDRPDMPVVMLSTYDNAPYVKRALADGASGY
                     LLKDATPEDLSQAINVALSGSGNVLSPRVIQNLFEEHESAGRDQNGRRGEFNLTQREH
                     DILELLAEGQANREIAGRLYLSEKTVKAHLAAIFRKLGVTNRTQAAMMAVQMGVGPIP
                     GALVHE"
     gene            complement(5750..6310)
                     /gene="dcd"
                     /locus_tag="VF195_12770"
     CDS             complement(5750..6310)
                     /gene="dcd"
                     /locus_tag="VF195_12770"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEX6581729.1"
                     /translation="MILSDRTIKEEIDAGRIVIDPFDPASVQPSSVDLHVDAEFRVFA
                     NNRYPYIDVKQEQPDLTELIETKPDEPFILHPGEFVLGSTLERVALPNDLVARLEGKS
                     SLGRLGLLIHSTAGYVDPGWDGYLTLELSNVANLPITLYPGMKIGQISFFRLTTEAET
                     PYGGAGSKYQGQRGPTASRFFEDFRA"
     gene            complement(6325..7362)
                     /gene="trpD"
                     /locus_tag="VF195_12775"
     CDS             complement(6325..7362)
                     /gene="trpD"
                     /locus_tag="VF195_12775"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX6581730.1"
                     /translation="MAEPDPDLWPRTLQRLASHEALSADDAAEVMRAIMSGDVTPAQV
                     GGFLMALRTKGETVDELDGLARTALEFANPVSPPAPVLDTCGTGGDRSGTFNISTVSA
                     IVVAGAGVPVAKHGNRAASGRCGSADLLEELGVAIDLDAAGVERCLAEAGIGFMFAPV
                     FHPAWGHAAPVRRELRVPTSFNFLGPLTNPARPAAQVVGVSDERMLPLMAEVLARRGT
                     RAKLFRGEDGIDELTTTGPSVVLDVRSGEVIESRLDPADLDIARASREDLHGGDPPES
                     ARVARAVLDGDHGPARDVVLLNAGAALEVAGASSDLDEGIALASSSIDEGKAGATLQR
                     WIAVSNSDLGA"
     gene            7453..7523
                     /locus_tag="VF195_12780"
     tRNA            7453..7523
                     /locus_tag="VF195_12780"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7485..7487,aa:Gly,seq:ccc)
     gene            complement(8060..8812)
                     /locus_tag="VF195_12785"
     CDS             complement(8060..8812)
                     /locus_tag="VF195_12785"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6581731.1"
                     /translation="MTGFDFEVVDATDYDELRPEYSPEAVAWVAERGGLVPGSSMVDL
                     AAGTGRLSGRFIQLGVDVVAVEPAANMRAVLEDRFPTVRAIVARAESMPFDDGAIDLV
                     VVGNAFHHFEREAAMAEIHRVLRPRGTLAVFWAWPAEEEQRKIPGIRAIWEAVEETRG
                     ESAIASAYRSWAEPPATVEGFDPFERREFPTTHSVPAARLADLYATSSDVVSLPPESR
                     EGLLARIREIARTLPEMLELPGKTVVDLCFAR"
     gene            complement(8887..9396)
                     /locus_tag="VF195_12790"
     CDS             complement(8887..9396)
                     /locus_tag="VF195_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017984205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581732.1"
                     /translation="MTSSGTAVVTLPAETQILITREFAAPKHLVYRAYTEPDLIARWW
                     SGERGEVTSVDVDLRVGGAWRYVMTANEGYEVAFHGEFREIVPNERLVNTEVYEMPED
                     FPLAGTPEAEEGTLNTITFAEVDGRTTLTTFVECHTKEVRDAIVDSGMEGGMQEAMDR
                     LEQIAVSLA"
     gene            complement(9386..9775)
                     /locus_tag="VF195_12795"
     CDS             complement(9386..9775)
                     /locus_tag="VF195_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6581733.1"
                     /translation="MARVATTADAFNAVAEPRRRQILDALAGGERPVNDLVRRLGLAQ
                     PLVSKHLRVLREVGLVDVRDEGRQRIYRLNGRSLKPIHDWVKNYEMAWAERFDRLDVV
                     LEELKGAEHIKGAEHTGKERGDGGDDE"
     gene            complement(9898..10587)
                     /gene="nth"
                     /locus_tag="VF195_12800"
     CDS             complement(9898..10587)
                     /gene="nth"
                     /locus_tag="VF195_12800"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX6581734.1"
                     /translation="MTEPASLGVLRRRARTVLSRLRKRYGDLVPPRRSDPLEELILTV
                     LSQHTSDVNAERAFEGLRAALPTWDAVVRAPTRSVADAIRSGGLADTKAPRIQAILRE
                     VEARRGAFDLSALAELSDDEARAELVSYPGVGPKTAAVVLSFSLGRDAMPVDTHVHRV
                     TRRLGLVPPKTSAERADRLLHELVPEGLRTPLHVALIRLGREICKAPTPRCKVCPLKD
                     ICPTAPRFLDR"
     gene            complement(10584..11963)
                     /gene="dnaB"
                     /locus_tag="VF195_12805"
     CDS             complement(10584..11963)
                     /gene="dnaB"
                     /locus_tag="VF195_12805"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX6581735.1"
                     /translation="MSGIVRTADDVVRLPRNGDHRVPPHNVEAEAAALGSMMLSAEAI
                     ADVVELLQAEDFYRSANGRIFEVLHELYARGEPVDQLSAVEALRRKGTLDDVGGPLYI
                     RDLTDQVPTPASAAHYARIVAQAALLRRLIGAAADVMDMAYSAPEEPEAVADAAEQRI
                     YDVARRDDRDEVATLRELIDGAMLELEQIQHREAAYTGLPTGFRDLDELTSGLQPGNL
                     VIVAARPGIGKSSLAMNIAHNVAVTGGSVAVFSLEMSRHEVGMRMLCSAARVPWDRIR
                     SKRVGPDDWVRVVQAAERLHDAPLSIVDSGNVNIVDVRAKARRMRSSKTGLALIIVDY
                     LQLMTSAAIRRPDNRQQEVAEISRSLKLLAKELGIPVLALSQLNRNPETRADKRPQLS
                     DLRESGSLEQDSDVVAFIHRDDADPEKKKEAELILAKHRNGPTGSIKLMFEPSLTQFR
                     NAARGDVPA"
BASE COUNT         1893 a         4352 c         4003 g         2039 t
ORIGIN      
        1 cgctgatgat gtagacggcc cggtcggcag cgctccgttc cgctcctcgt tcgtcgtgat
       61 ccttcatccg actcctcctc atcgcacgcc cagccgcttc cgtggcgacc ccttcgacgc
      121 ctcgtgcagc tgccgcagga gctccttctc ctctcgcgag accttctcgg ggacgtcgac
      181 cagcaccttc gcaaggaggt cgccgcgccc tcccttcctc ggtgcccccc ggcccttcac
      241 ccggaacgtc ttcccgctcg gcgtgccggc gggcaccttc agggtgaccg gcccgttcaa
      301 ggtcggcacc tccacgttcg ccccgagggt cgcctccgtg taggagatcg gcaggtcgag
      361 ggtcaggtcg tcgcccttcc gaccgaacac tggatgcgac tcgacggcga ccttcacgaa
      421 gaggtcgccg ggacgcccgc ccgccggacc cggctcgccc ttgccggcga gccggatccg
      481 cgccccgtcg cgcacgccgg ccgggatctt cacgtgcaag gtgcgggtcc ggcgctccgc
      541 gccggacccg ccgcacttgg tgcacgggga ctcgacgatc ctcccggcgc cgccgcagcg
      601 cgggcagggc cgcgacatcg agaagaatcc ctggttctcc gcgatctccc cgctgcccgc
      661 gcactgggga caggtgatcg gctgcgttcc cggctcggct cccgatccgt ggcagcgagc
      721 gcacacggcc ggcccgtcga tcttgatagg cagggtcgtg cccgccacgg cgtcctcgaa
      781 cgagatcgag accgaggtct ccaggtccgc gcctcgctgc gcggcgggac ggcggccgct
      841 ccgcctcccc gccccgccga agagcccgcc gaacagatcg ccgaggtcgt cgacgttgac
      901 ctgctcgtac cgcacgccac ccggccatcc ggctccggcg gcgccgggtg cccccgcacc
      961 cggccggccg aagcccggac cgaatcccga cgcgctcatg tcgcgcacct ggtcgtagga
     1021 cttgcgcttc tcggcgtcgc cgagcacgtc gtacgcggcg gagatctctt tgaaccggtt
     1081 ctccgcgtcg gggtcgccct gcttcgtgtc agggtgatgc tgctgggcga gtgtgcggta
     1141 ggccttcttc acctccgcgg cggaggcgtt cttgggcacg ccgaggaccg cgtagtagtc
     1201 cttgtcgaac cactccctgc gaacctcgtc cgccatgtct cacttcctct cgacccgaac
     1261 cccggccggc cggaggaccc ggcctttgag cgtgtacccc gggcgcagca cgtcttcgac
     1321 caccggatcg ccatcgcctt cgcccgactg catcagcgct tcatggagct ccggatcgaa
     1381 cggcttgccc tgcgcctcca tccgctccag gccttccgca cgcagggtgt cgatgagctt
     1441 ggcgtagacc agctccaccc cgtgcaggaa gcggtcgaag tcgggccgat cctccgcatg
     1501 catcagggcg aggtcgaagt cgtcgaggac ctcgagcaac cgacgcagca ccggttgcgc
     1561 ggccagctcg acggcggacg tctgctcccg cagcacgcgc ttgcggtagt tgtcgaactc
     1621 cgcctgtaga cgctgcaggt gctcgcggta ctgatccgct tcggccagcg ccgtctggac
     1681 gtcggccgtc ccgacgtcgg gtcccgcgtc ggacgcggcc gcctccggcc tcgcttcgga
     1741 agcggccgcc tcccgggcgg ccgcttcctc aaccgatccc tgatcgggac gatcctccgt
     1801 caccgggacc ggacctcgat cgtcctgggc ttcgcttgct ccgtcttcgg taccgagacg
     1861 gtaaggacgc ccttatcgaa cgtcgcctcg atcctctcgg tatcggccgt ctgcggcagc
     1921 cgcatcgacc tcgcgaacgt tccgaagctc cgctcgatcc ggtgccatgt ctcaccccgg
     1981 tcctcggccg agaactcgcg cttcccggtg atcgagagca tcccgtcctc gaacgtgacg
     2041 ttcacgtcat cggggtcgag gccgggcagg tcgaccgtca catcgaaccg atcctgagat
     2101 tcgcgcacgt cgagcgcggg cacccacgtg tcccgcgaga acgcgcggcc ggcgaccctg
     2161 tcgaactcgt cttcgacgga ccgcatgtcc cggaacagat cccacttggc gagcatcacg
     2221 atgcacctcc ttcgtccacg atctcgcctt caaccacatc ctcgtcgtca tccggctgct
     2281 cgccggccga cgccgaggtc tcgtcgccgc cgctccccgc ggcggcttgc gcctgcgcgg
     2341 cctgctcgtg catcgactgg ccgacccgct gccacacctg catcagggcg tcgctcgcgg
     2401 tgcgcaggct ttcggtctcc gcggattcgt ccttcagcgc ctgcttgagc tcgtcgttct
     2461 tcgacgtcag ctcggccttg tccgcgtcgg agatgttctc cgcgttgtct ctgaggaagg
     2521 agtcgacctg gtaggtcagc tggtcggcct ggttccgtgc ctcggctgcc tcccggcgcg
     2581 tgtggtcctc ggcggcgaac ccctcggcct ccttcatcat gcggtcgatc tcctccttcg
     2641 agagcgccgt cccgccggtg atcgtcatgg cctgctcctt gccggacgcg agatccttgg
     2701 cgctcacgtt cacgatgccg ttcgcgtcga tgtcgaacgc gacctcgatc tgcggcaccc
     2761 cgcgaggcgc cggggggatc ccggtcaggt ggaagcggcc gagcgacttc acaccgggcg
     2821 acatcaccgt ctcggcctct ccctgcagga cgtggatctc caccgtcgtc tggttgtcgt
     2881 cggcggtcgt gaagatctcg gacttgcgcg tggggatcgt cgtgttccgc tcgatcagct
     2941 tggtgaacag gcctcccttg gtctcgacac cgagcgtcag cggcgtcacg tcgagcagca
     3001 ggatgtcctt cacgtcgccc ttcaggacgc ccgcctggat cgcggcgccg acggcgacca
     3061 cctcgtcggg attgactccc ttgtgcgggt ccttgccgcc ggtcaggtcc ttgacgagtt
     3121 cctggatcat cggcatccgt gtcgatccac cgacgaggat cacgtgatcg atcgcgccgg
     3181 cgtccttgcc ccagtccgcg atggcctgct cgaagggtcc cttgcaccgg tcgacgaggt
     3241 cggccgtcat ccgctcgaat tccgtgcggg acagcttctc ctcgaggtgg agcggcccgt
     3301 ccggacccgc gctgatgaag ggcaggttga tcgtggtctc ggtcacctgg gacagctcga
     3361 tcttcgcctt ctcggccgcc tccttgaggc gctgcatcgc catccggtcc ttggccagat
     3421 cgatcgagta gttccccttg aaccggtcgg ccagccagtc gatgacccgc tggtcccagt
     3481 cgtcaccgcc gagctgcgtg tcgccgtggg tggccttgac ctccaccacg ccttcgccga
     3541 tctcgaggag ggagacgtcg aacgttcctc ctccgaggtc gaagacgagg atcgtctgct
     3601 cggtctgttg cttgtccagc ccgtaggcga gggccgcggc cgtcggctcg ttgatgatcc
     3661 ggagcacctc gaggccggcg atctgcccgg cctccttcgt cgcctgtcgc tgcgcgtcgt
     3721 cgaagtaggc ggggaccgtg atcacggcct gcgtgacggt gtcgcccagg taggcctccg
     3781 cgtcccgctt gagcttgttc aggatctgcg ccgagatctc ctggggtgtc caccccttcc
     3841 cgtccacgtc gacggaccag tccttggccc ccatgtgccg cttgaccgag cggatcgtcc
     3901 gatccgggtt cgtgatggcc tgtcgcttcg cgacctcgcc gacgaggatc tcgccggtct
     3961 tcgagaaccc gaccaccgac ggggtcgtgc gtgacccctc cgcgttcgga accacgaccg
     4021 gctccccacc ctcgagtacc gcgacgaccg aattggtcgt gcccagatcg actcccaccg
     4081 ctcgtgccat cgggcgatcc ctccttgtga gttgctcaga gtccgtagaa ctcctgcgtt
     4141 cggatgcctc taccatagaa cctaagtgcg gtattgacaa cttttctccc tcatcctcga
     4201 gagagttgcg ggccaagtcg tgggacgagc cccgaccggt cgacgggctt ccgacggaag
     4261 tcccttcgcg ccacctgcct caaggcgttc aagcccaacg ccttttcgtc cgatggatgg
     4321 actagaccca acggaccgga ccgcccgaca cctccatcga aaggatgaga gtggctacat
     4381 cgagcacgac gttgcgcacc cgacggggcg tgcatccgac cgtggcccat acggtcgata
     4441 cgctgctgtt gcaggagcgc acggactggg cgatccacat ccatgacggc ctcacccagt
     4501 ccgtcacgag cgcgatcctc gagctccagg gcctgcagca caggatccgc acggaaccgg
     4561 agctcgtctg ctcgtccttg aaagagatcg aagacgcgat ccggaacgac ctgaagcaga
     4621 tccgggagat cctgttcgag ctcgacggcg ggacaccgca ccgcgagcct ccgctcgcga
     4681 cgttcgtgag cgagctcgtg gagcgctggc acctgtcagc gcgggtctcg atcgagggag
     4741 aggtggagcg gatcccggac gtgacgctgg agaccgcgaa cgcgatcgtc gccgaggccc
     4801 tggccaacgc ggcgaagcat tcgggcgctc cggaggtgtc ggtccgcatc atcgccggac
     4861 ccgaggagct ccggatcgag gtggaggacc gcggcagcgg cgtcgtcaat cccaccgacg
     4921 aagagatgca cttcggcctg cgcttgatgc gcagccgagc agaacggatc ggcggcacca
     4981 tcgagatcga ttcgaagccc ggaagcggca cccgcgtggt cgccgtgctc cccgtcggac
     5041 ggcgaggtga tgaaggatga tgaagatcct gatcgtggac gaccacgcgc ttgtgcgtcg
     5101 tggtctgcat tacgtggtca aggaaggatt cccggacgcg gaggtcaccg aggcggaaag
     5161 cgccgcggcg gccctcgagc acatccacaa cggcaaggag atcgacctcg cgttggtcga
     5221 cgtacggatg ccggatctgg acggactgga gctgctccgc gccctcaaga ccgatcgtcc
     5281 cgatatgccg gtggtcatgc tctcgaccta cgacaacgcg ccgtacgtga agcgcgcgct
     5341 cgccgacgga gcctccggct acctgctgaa ggacgccacg cccgaagacc tctcgcaggc
     5401 gatcaacgta gcgctctcgg gcagtgggaa cgtcctgtcg ccgcgggtga tccagaacct
     5461 cttcgaggag cacgagtccg cggggcgcga ccagaacgga cgccgcggcg aattcaacct
     5521 cacacagcga gagcacgaca tcctcgagct cctcgccgag gggcaggcca accgcgagat
     5581 cgcgggacgc ctctacctgt cggagaagac cgtcaaggcc cacctcgccg cgatcttccg
     5641 caagctcggc gtcaccaacc ggacacaggc cgcgatgatg gccgtccaga tgggtgtggg
     5701 cccgatcccg ggggcgctcg tccacgagta gccggaccgg tgctgccggt caggcccgga
     5761 agtcctcgaa gaaccgactc gccgtgggcc ctcgctggcc ctggtacttg ctgcccgcgc
     5821 ccccgtacgg ggtctcggcc tcggtggtca atcggaagaa cgatatctgt ccgatcttca
     5881 tccccgggta gagggtgatg ggcaggttcg cgacgttcga gagctcgagc gtcaagtagc
     5941 cgtcccatcc tgggtccacg tacccggcgg tcgagtggat caagaggccc agtcggccca
     6001 gcgatgactt cccctccagc cgcgcaacga gatcgttcgg cagtgccacg cgctcgagcg
     6061 tcgatcccag gacgaactcg cccggatgga ggatgaacgg ctcgtcgggc ttggtctcga
     6121 tcagttcggt cagatcgggc tgctcctgct tcacgtcgat gtaggggtag cggttgttcg
     6181 cgaacacccg gaactcggca tcgacgtgca ggtccaccga ggagggctgc acgctcgccg
     6241 ggtcgaacgg atcgatcacg atgcggccgg cgtcgatctc ctccttgatc gtgcggtcgg
     6301 acaggatcat cggcgcggat gctactaggc gccgagatcg gagttggaga ccgcgatcca
     6361 gcgctggagt gtcgctccgg ccttcccttc gtcgatcgaa gaggaggcca gcgcgatccc
     6421 ttcgtcgaga tcggatgagg cgcccgcgac ctcgagtgcg gcgcccgcgt tcagcagaac
     6481 aacgtcgcgg gcgggaccgt ggtcaccgtc cagcacggca cgagcgacgc gcgccgactc
     6541 gggggggtcg ccgccgtgga gatcctcccg ggacgctcgc gcgatgtcga gatcggcggg
     6601 atccagacga ctctcgatga cctccccgct tcgcacgtcg aggaccaccg acggccccgt
     6661 ggtggtgagc tcgtcgatgc cgtcctcgcc tcggaagagc ttcgcgcggg ttccgcggcg
     6721 ggcgagcacc tcggccatca acgggagcat gcgctcgtcc gagacgccga cgacctgcgc
     6781 cgccgggcga gccggattcg tgagcggtcc gaggaagttg aaggacgtgg gcacccggag
     6841 ctcccggcgc accggcgccg catggcccca cgcaggatgg aagaccgggg cgaacatgaa
     6901 cccgatcccc gcctcggcca ggcatcgctc gacaccggcg gcatcgaggt cgatggcgac
     6961 cccgagctcc tcgaggagat ccgccgaccc gcaccgacca gaggcggcgc ggttgccgtg
     7021 cttcgcgacc ggaacccccg cgccagccac gacgatcgcg gagacggtcg agatgttgaa
     7081 cgtgccgctc cggtctcctc cggttccgca cgtgtcgagc accggcgccg gaggcgagac
     7141 ggggttcgcg aactcgagcg cggtgcgggc gagcccgtcg agctcgtcga cggtctcccc
     7201 cttcgtccgc agggccatca ggaagccacc gacctgcgcg ggggtcacgt cgcccgacat
     7261 gatggcacgc atgacctcgg cggcgtcgtc ggccgagagc gcctcgtggg acgcgagccg
     7321 ctgcagcgtg cgcggccaga ggtccgggtc gggctcggcc atgaggcagc gcacgctagc
     7381 acgtcgacct cgcgtggatg ggccccaagg cctcgtctac actgtcgctc cgccgccctc
     7441 gggtggcgcc aagcgggtgt ggttcaatgg cagaacgcga gcttcccaag ctcgaaacgg
     7501 gggttcgatt cccctcaccc gctctctcag cccgtcgcga tgcacttgag ccccgtggcg
     7561 atcgtattca tctgatcgac ggccagctcc agatcctcgc ctccgccgac cagcccagac
     7621 gtgctcagaa cgagacaccg caccggagca ccgttcagcc gatagaggta gcccaccttc
     7681 tttccccacc gcgagcgaag gacagatcga ctgaactcag tccagccgac cacatgccgc
     7741 cgccaaccgt tgagaacgac gacgccttcc ccccgaaccc cactcttgaa cgggccgact
     7801 caaggagaag tagcttcctg ctcccaacac cgccacacgg atgactctca ttccccacga
     7861 ggcgtccatc accgggagca gcgtcgcgaa cgcggcaagc ccagcccccc aaagaacagc
     7921 ccatgtggtc aagcagtacc tttgccaagc ccggagaggg cagccgccag aggacacctc
     7981 aacgcgtcga gatccatccc tctctttcat cgtgctccgc acccttactc aggtctcgcc
     8041 tgagaacgtc gcctccgcct catctggcga agcagagatc caccacggtc ttccccggca
     8101 gctccagcat ttccggcaaa gttcgggcga tctcgcggat ccgagcgagg agtccttcgc
     8161 gcgattcggg gggaagggac acgacgtcgc tggatgtcgc gtacagatcc gcgagccgcg
     8221 ccgccggtac tgagtgggtt gtggggaact cccggcgctc gaacggatcg aatccctcca
     8281 ccgttgccgg cggctcagcc cacgatcggt acgcggacgc gatcgcggac tcaccccggg
     8341 tctcctcgac ggcttcccag atcgctcgga tgcccgggat cttccgctgc tcctcctcag
     8401 cgggccaggc ccagaacacg gcgagggtac ctctggggcg caggacccga tggatctccg
     8461 ccatcgcagc ctcgcgctcg aagtggtgga acgcgttgcc gacgacgacc aggtcgatcg
     8521 ccccgtcatc gaacggcatc gactccgccc tcgccacgat cgcccggacc gtggggaacc
     8581 ggtcctcgag taccgcccgc atgttcgccg caggctccac agcgaccacg tcgacaccga
     8641 gctggatgaa tcgacccgag aggcgccccg tccccgccgc caggtcgacc atgctcgaac
     8701 cggggacgag gccgccacgc tcggcgaccc aggcgacggc ctccggcgag tactcgggcc
     8761 ggagctcgtc gtagtcggtc gcgtcgacta cctcgaagtc gaagcccgtc atgagcgaga
     8821 gcctagaagg agcgggccac cgaaggacgt cgtccctggt ggcccgctgc acgcgagcgt
     8881 gcgatcctat gcgagcgaga ccgcgatctg ctcgaggcgg tccatcgcct cctgcatccc
     8941 gccctccatg ccggagtcga cgatcgcgtc ccggacctcc ttggtgtggc actcgacgaa
     9001 ggtcgtgagg gtggtgcgcc cgtccacctc cgcgaacgtg atcgtgttca gcgttccctc
     9061 ctccgcctcg ggtgttcccg ccagcgggaa gtcctcgggc atctcgtaga cctcggtgtt
     9121 cacgagccgc tcgttcggga cgatctcgcg gaactcgccg tggaacgcga cctcgtagcc
     9181 ctcgttcgcc gtcatcacgt agcgccatgc gccgcccaca cgcaggtcga cgtcgacgct
     9241 cgtgacctcg ccccgctcgc cgctccacca gcgcgcgatc aggtccggct ccgtgtaggc
     9301 ccgatacacc aggtgcttcg gtgcggcgaa ctcccgcgtg atcaggatct gcgtctccgc
     9361 cgggagcgtc acgaccgcgg taccgctact cgtcatcgcc accatctcct cgctcctttc
     9421 ctgtgtgctc cgctcctttg atgtgctccg ctcctttgag ctcttccagg accacatcca
     9481 gccgatcgaa gcgttccgcc cacgccatct cgtagttctt gacccagtcg tggatcggct
     9541 tgagggaacg accgttgagt cgatagatcc gctgcctccc ttcgtctcgc acgtccacca
     9601 atcccacctc cctcagcacc cgcaggtgct tcgacaccag gggctgcgcc aggccgagcc
     9661 gccgaacgag gtcgttcacc ggacgctccc cgccggcgag ggcgtccagg atctgtcgcc
     9721 gccggggctc cgccaccgcg ttgaacgcat ccgccgtcgt cgcgacccgt gccatgggag
     9781 gactatatat gcccatatcg gtatatgtca agctcccccg gggcgcggcc ggaaagggag
     9841 cgaagcaggc cggcagaggg aaggaggaga cactcggagg tcagtcggcg acgagcccta
     9901 cctatcgagg aatcgcggcg ccgtcggaca gatgtccttc agcggacaga ccttgcaccg
     9961 gggcgtgggg gccttgcaga tctcgcggcc gagccggatc aacgccacgt gcagcggcgt
    10021 ccggaggccc tcgggcacca actcgtgcag gagccggtcg gcccgttccg cagaggtctt
    10081 cggagggacc aggccgagtc gccgggtgac gcgatgcacg tgcgtgtcca ccggcatggc
    10141 gtcacgcccg agcgagaagg agagcaccac ggccgcggtc ttcggaccga cgccgggata
    10201 tgacacgagc tccgccctcg cctcgtcgtc ggacagctcc gcgagggcag agaggtcgaa
    10261 cgcgccgcgc cgtgcctcca cctcgcgcag gatcgcctgg atccggggcg ccttcgtgtc
    10321 cgcgaggcca ccggagcgga tcgcgtccgc cacggaccgg gtcggcgcgc gcacgacggc
    10381 gtcccatgtc gggagagcgg ctcggaggcc ctcgaacgcg cgctcggcgt tgacgtcgct
    10441 ggtgtgctgc gacagcacgg tgaggatcag ctcctcgagc ggatcggagc gccgtggggg
    10501 cacgaggtcg ccgtagcgct tccggagccg gctgagcact gtgcgcgccc gccgccggag
    10561 gacgcccagc gaagcgggtt cggtcacgcc gggacgtcgc cgcgggcggc gttgcggaac
    10621 tgcgtgagcg acggctcgaa catgagcttg atggagcccg tcggcccgtt gcggtgcttc
    10681 gcgaggatca actcggcttc cttcttcttc tccggatcgg cgtcgtcacg atggatgaac
    10741 gcgacgacat cagagtcctg ctcgagcgag ccactctcac ggagatccga gagctggggt
    10801 cgcttgtcgg cccgggtctc gggattccgg ttgagctggg agagggccag gaccgggatg
    10861 ccgagctcct tggcgagcag cttcagcgac cggctgatct ccgcgacctc ctgctgccgg
    10921 ttgtcgggcc gccggatcgc ggccgatgtc atcagctgga ggtagtccac gatgatgagg
    10981 gcgagcccgg tcttgctgct ccgcatccgc cgtgccttgg ctcggacgtc gacgatgttc
    11041 acgttgccgg agtccacgat ggacaagggg gcatcgtgca agcgctcggc ggcctggacc
    11101 acgcggaccc agtcatcggg cccgacacgc ttgctccgga tccgatccca cggaacccgg
    11161 gccgccgagc agagcatccg catgccgacc tcgtgacgcg acatctccag cgagaagacc
    11221 gcgaccgagc cgccggtcac cgcgacgttg tgcgcgatgt tcatggcgag ggaggacttc
    11281 ccgatgccgg gacgggcggc gacgatcacg aggttgccgg gctgcaggcc tgacgtgagt
    11341 tcgtcgaggt cacggaaccc cgtgggaaga cccgtgtacg cggcctcgcg gtgctggatc
    11401 tgctcgagct cgagcatcgc gccgtcgatc agctcgcgga gggtggctac ctcgtcccga
    11461 tcgtcccgcc gcgcgacgtc gtagatacgt tgctcggcgg cgtcggcgac cgcctcgggc
    11521 tcctccgggg ccgaataggc catgtccatc acgtcggcgg ccgccccgat cagtctcctg
    11581 agcagggcgg cctgcgcgac gatccgcgcg tagtgagcgg cggaggccgg ggtcgggacc
    11641 tgatcggtca ggtcccggat gtacagaggg ccgccgacgt cgtcgagcgt ccccttccgg
    11701 cggagggctt ccacggcgga cagctgatcg acgggttccc cacgcgcgta cagctcgtga
    11761 agcacctcga agatccgccc gttcgccgaa cgatagaagt cctcggcctg cagcagctcg
    11821 acgacgtcgg cgatggcctc ggccgacagc atcatcgacc cgagggccgc ggcctccgcc
    11881 tccacgttgt ggggcggaac gcggtgatcc ccgttgcgag gaaggcggac gacgtcgtcg
    11941 gccgtgcgga cgataccgct caccccctgt cacctgaccc ggtctccgga ttcgggacgc
    12001 tacgcccggg gaggtcgggc gaacagtgga gaacgttggg ggattgttcc cgtgcaggtc
    12061 gcatcggaat gtttcctgtc actccaccgg tgacctgcgg gaacgggttg gggacaacat
    12121 ggggataact gtgagtatcg ccaccgacac gcttcggggc gtctcagagc ccttgcgaga
    12181 gagtgcgtcc tggcgaggcg tccgaacgct cgttcggaga ggcgtgtcat gtggcccgcg
    12241 gacgggccac atgacggggg cctattcctg ggctcgaacg tcgatcg
//
LOCUS       DASUYD010000244        12495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_411848, whole genome shotgun sequence.
ACCESSION   DASUYD010000244 DASUYD010000000
VERSION     DASUYD010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 12495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12495
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411848"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1350)
                     /locus_tag="VF195_12810"
     CDS             complement(148..1350)
                     /locus_tag="VF195_12810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581736.1"
                     /translation="MSDVRDEALGAKLQQEANRIESVPVDRLPDVLRRGGRLRAIRFT
                     AIGAALAVFAGAVSLAGLSLRNETIESEVTLRYSSRAAPWTFNYPSGWTTKTISRVGP
                     ELRTNVLRTTVVNGPMPSLADDFGPNSVGNDALTSEAGDAGAIVLVVRFWGGKPSTDD
                     YPHLGPGAFGADAESPGWTIRERTRCDGSLCFVIVEWLGPSVSDEDRDVAAEIARSVR
                     LADVDRWTESDGVSTTLHDEARRYVVTYPTTWVVADENLTPWLSSPSEILSLGTFPLR
                     ASAHPDDGFRLFDAPVAPAALQDMRSEDAFLSLQDSGGVFAGSTESRPDHFGPLGCDE
                     SIYGCRPSQELPEAWRDAPFRAWWIPFEDAGRAFHLFVAIGTEATPELRQQTWAVADS
                     LSFEPASA"
     gene            complement(1347..1826)
                     /locus_tag="VF195_12815"
     CDS             complement(1347..1826)
                     /locus_tag="VF195_12815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581737.1"
                     /translation="MPDRAGFEAFYAAHLPRVVRACALVLLDRGDAEDVAAEAFGRLW
                     SHWGQIHGEDHAGGYVFKTAMRLCAKRRRRAAREVVGTVPERSRPTDADARLSVVDAL
                     RGLPLRQRQSVVLRDWAGFETGEVARMLGTKNSTVRVHLTRGRAALRDALRVEERER"
     gene            complement(1884..2282)
                     /locus_tag="VF195_12820"
     CDS             complement(1884..2282)
                     /locus_tag="VF195_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018772663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581738.1"
                     /translation="MAETLIVVDTSEIREGKRGELERGIAELAAFVETNETDPISYQV
                     FFGDDGDLMTVLQIHPDSASMERHMELAGPVFARFADLVVLRTVDIYGSPSEKVVEQL
                     RRKAELLGTATVTVHDRQAGFARFGSHLVG"
     gene            complement(2633..3916)
                     /locus_tag="VF195_12825"
     CDS             complement(2633..3916)
                     /locus_tag="VF195_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581739.1"
                     /translation="MRRLGVTIVMLLVACTSEPSPPSPSGSPRETPSGDPVEVTTGPT
                     PAAEAMRRLCVAPDIEVDPVREMPTPPAIAEVADQVESVRGLDFDREVNVEPITEPEM
                     DQRLADYLGTYYPKRFYARRSEAWQTIGALPRDVGYLEAIDAYQQGQVLGFYNSQNEE
                     LVYTGDAELNRIEQFVLAHELTHAIDDQHFDLDRLDGLVARCEEERFQAALGIVEGSA
                     NHFATQVLIRFPIQEVGPIPGDDPGEVPPFIVEMQAFPYTTGQRFADALSDADGPSAI
                     DGAMERFPTTTEQVMHPTKFPHEAGESVDVADFAPTFGSRWRDLDVMVVGELWLKVLL
                     HLRLDDQVAASAAAGWGGGTYRAWTDGDDVAVIMSTVWDTPKDAQEFSRELDRWVSRG
                     TLPGLVMDANGTTVHAGFASREPLMGAVLSILRSL"
     gene            complement(3913..4080)
                     /locus_tag="VF195_12830"
     CDS             complement(3913..4080)
                     /locus_tag="VF195_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581740.1"
                     /translation="MIAYLAAVVLWVAAVVTLVVSTAGFLESTTLLTASAVLSGLAIV
                     AAVSAVVSRRR"
     gene            complement(4077..5381)
                     /locus_tag="VF195_12835"
     CDS             complement(4077..5381)
                     /locus_tag="VF195_12835"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017546256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HEX6581741.1"
                     /translation="MEAVTSLAKRRGIAFQSSEIYGGLRSSWDYGPLGTEIKRNVREQ
                     WWRSTVQLRDDVVGLDASVIMSPKVWEASGHLEVFTDPLVECLNCHQRFRADELPEPA
                     ACPNCGQTAFTQPRNFHLMFRTNMGPIEDAENTVYLRPETAQGMFVDFATIQQVSRKK
                     VPFGIAQIGRSFRNEITPGNFIFRQREFEQMEMEFFVEPGTDEDWHEFWIGERMRWWT
                     DLGLREDHVRIREHDADELSHYSKRTVDIEYRFPFTDWGELEGIANRTDFDLRAHQAA
                     SGEDLTYFDQEKNERYHPYVIEPAAGVDRAVLAFLLDAYREEEAPTAKGGTEKRTYLA
                     LDRRLAPTKVAVLPLSRNEKLAPEARRVFDLVKSEWMCDFDDAGSIGRRYRRQDEVGT
                     PFCVTVDFDSLEDAQVTVRERDSMGQDRLAIDGLIDYLTERL"
     gene            complement(5506..6966)
                     /locus_tag="VF195_12840"
     CDS             complement(5506..6966)
                     /locus_tag="VF195_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581742.1"
                     /translation="MIARFTRDGASKYVWAVMALAAIGGLAFAIMNGNDALERERANA
                     QGRAVRYVEQELAPRLEGSALDGPITGQSAVSLEAAVERTILADERLTRVRIWSAEGP
                     LLFSTDQADTPGSNAGLNDQLLRRVAREGVLTRSGISDTGGEDDPERSLVRTYVPLEA
                     GAIAEIDQTDEGTLAAVRTEWMYYQILAGAVLLLSLVLTGLSLRDPIEPINTGVPFAS
                     SSVPAGFSLIDNDRLHAVQEVYRLASERVGRLQQKLEESEEARRRLEAGMQQALSKAA
                     GSSGAVSPLPVADTPPEPAIVQVPESDVVEMPSDEAWTASPAGPLARASRDQKPPPAT
                     ARAPRPPADRQKRVRKPKPEKPAKQEPPKEEPPKEEPVKTQPTAASDPILVPEPTSVP
                     ESRVAAMATTQASPPPAPTAADPELADAEAHEAALEMFIRLTESDRQPHDASEVDQGA
                     VRAALARTAARKKPGGERLQPHEGPPGGSPGGPPRS"
     gene            complement(7128..7871)
                     /locus_tag="VF195_12845"
     CDS             complement(7128..7871)
                     /locus_tag="VF195_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEX6581743.1"
                     /translation="MSSYLQDLDLDRIPAHVGIVMDGNGRWAEQRGLPRTEGHGAGED
                     ALWDTIQGAVAVGVRWLTVFAFSTENWRRPPGEVRYLMNFNRTMLRRRRDALHEMNVR
                     IRFIGRRNWRIPRSVLKEIRAAEAMTAANTGTTLSIAFNYGGRAEIVDAVKDIAREVR
                     AGTLRPEGISESTIAERLYAADTPPPDLLIRTSGEQRISNFLLWEVAYSELWFTPVFW
                     PDFDREILYEAIRDFQKRSRRFGGVGDEP"
     gene            complement(7868..8608)
                     /gene="recO"
                     /locus_tag="VF195_12850"
     CDS             complement(7868..8608)
                     /gene="recO"
                     /locus_tag="VF195_12850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEX6581744.1"
                     /translation="MPPYKEQGVVLRSAKLGEADKIVTIMTQGSGKVRGVAKGIRKTT
                     SRFGARLEPFTHVNLMLYRGRGSLDTITQAEIIAPHRAIRDDLSLFAAGETMLEAVDR
                     VAEEHERNVRVVLLLLSGLRSLEARPIDPAAVAESFLLKLLSISGFHPSLTACAICGS
                     TDVQLFSAGLGGAVCAADAEPGAGHVTAAALAFLGALALADLADAGQTTAPESRTRKE
                     ARGLLFGFAEYHLERRMKSVPMLVRTAT"
     gene            complement(8712..9077)
                     /locus_tag="VF195_12855"
     CDS             complement(8712..9077)
                     /locus_tag="VF195_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX6581745.1"
                     /translation="MGKVSKDSMAEQFEGPGFEGRYGETEGYTIGFERYTEHSDMTPL
                     FVGLPNDQCQSPHWGYVFKGKLVYHTVEGDIEITDGEAYYVGPGHTPEIFPDTEIVEF
                     SPTAELNETMEVVTKNLEA"
     gene            complement(9215..10075)
                     /locus_tag="VF195_12860"
     CDS             complement(9215..10075)
                     /locus_tag="VF195_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6581746.1"
                     /translation="MTEERLPVRRTAKLFIAGEFPRSESGRSYEVFDADGELLAWASG
                     ASRKDLRDAVRAARAAQPGWAAKTAFNRAQILYRVAELMEGRRVQFEQELGDAGAEDP
                     ARGVSAAIDRWIWYAGWADKIHQIQGASNPVAGPYFNFTIPEPTGVVGIVAPQDQSLL
                     GLVSRVAPAIVSGNTAVVLASERSPLPAVSLGEVLATSDVPGGVVNILTGQTAELVPW
                     LAGHMDVNGLDATGVPDELRAQVEELSAENVKRVHRAPGADPFDEEAQSPYEVTAFVE
                     YKTVWHPMGA"
     gene            complement(10072..11592)
                     /locus_tag="VF195_12865"
     CDS             complement(10072..11592)
                     /locus_tag="VF195_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX6581747.1"
                     /translation="MIELDTTQARTPATPAWGYADAPESKDIVRLQDRYGLFIGGEFV
                     EPKSGRYMPTIDPSTEEVLAEVPEAGQEDAELAVTAARDAFESRWRDLPGRERAKYLF
                     RIARALQERAREFAVLESMNGGKPIKESRDIDLPLAAAHFFYYAGWADKLEYAFPGRD
                     ARPIGVAGQVIPWNFPMLMAAWKLAPALATGNTTVLKPAETTPLTALLLAEVIQAAEL
                     PPGTVNVLTGAGETGAMLVAADVDKVAFTGSTEVGKQIRRTIAGSHKRLTLELGGKAA
                     NIVFEDAPLDQAVEGVVNGIFFNQGHVCCAGSRLLVQEPIFEALLEKLKARLQTLRVG
                     NPLDKNTDIGAINSRAQLTRIEELVASGVEDGAEMYQPACDLPERGFWFRPTLFTGVS
                     QSHRIAREEIFGPVLSILTFRTPEEAVEKANNTPYGLSAGVWTEKGSRILWMAERLKA
                     GVVWANTFNRFDPSSPFGGYKESGFGREGGLHGLAPYLELTPREPAPGTAPGNGAS"
     gene            11761..12234
                     /locus_tag="VF195_12870"
     CDS             11761..12234
                     /locus_tag="VF195_12870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581748.1"
                     /translation="MTKRADVQEVGLMKRRSWIVAGSLTGLVACSALVALAPVLVRDL
                     RGPVYGDLALPALLVSGAAGIFFGGIVASRTVGTTPRRIALLGAACGAILGAVATGSV
                     FLAVEVAGDDWPHGLAAFVTGGIIGAVVGGFLGGAIGIVQVDSPAVDEGHAPRGG"
     gene            complement(12213..>12495)
                     /locus_tag="VF195_12875"
     CDS             complement(12213..>12495)
                     /locus_tag="VF195_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX6581749.1"
                     /translation="FAWSALLAVAAVGMLGTGVAFVLMGRLVGRVGATRASIVGYLIP
                     VVALLLGVHIRDDVVGPFALTGVVLVILGAYLTSRREVAVDADQPPRGA"
BASE COUNT         1901 a         4434 c         4190 g         1970 t
ORIGIN      
        1 tcgacggatg gcacacgcca aggacaaggg cgcgaagaac gtcaagaaga aggccgcgaa
       61 gggcatcaag gagaagcggg ccgagaagaa gctgaagcac gacgggcgcc gtcagacggg
      121 cgccgtcgag acgatcggcg aacggagcta ggcggaagcc ggctcgaacg agaggctgtc
      181 ggccacggcc cacgtctgtt ggcgcaactc gggtgttgcc tcggtgccga tggccacgaa
      241 gaggtggaag gcccgacccg catcctcgaa cgggatccac cacgctcgga agggagcgtc
      301 gcgccaggcc tccggcagct cctgcgacgg acggcagccg tagatcgact cgtcgcagcc
      361 cagtggtccg aagtggtccg ggcggctctc ggtcgatcct gcgaacaccc ctccactgtc
      421 ttgcagggat aggaaggcgt cctccgaccg catgtcctgg agtgcagccg gagcaaccgg
      481 cgcgtcgaac aaccggaacc cgtcatcggg gtgcgcactg gctcggagcg ggaaggtccc
      541 gagcgacaag atctctgacg gggagctgag ccacggcgtg aggttctcgt ccgcgacgac
      601 ccacgtcgtg gggtaggtca cgacatatcg gcgcgcctcg tcgtgcagcg tcgtggatac
      661 accgtcgctc tccgtccagc ggtcgacgtc cgccaatcga accgaacgag cgatctcggc
      721 cgcgacgtct cgatcctcgt ccgacaccga agggccgagc cactcgacga tcacgaagca
      781 cagcgaccca tcgcatcggg ttcgctcccg gatcgtccaa ccaggactct ccgcgtcggc
      841 cccgaaggcg cccggcccga ggtgcgggta gtcatccgtc gacggcttgc cgccccagaa
      901 gcggacgacg agaacgatcg cgcccgcatc accggcctca ctcgtcaacg cgtcgttccc
      961 aaccgagttc ggcccgaagt catccgcgag cgacggcatc ggtccgttca cgacggtcgt
     1021 cctcaggacg ttcgtcctga gctcggggcc gacgcggctg atcgtcttcg tcgtccagcc
     1081 cgacggatag ttgaacgtcc acggagccgc tcgggacgag tacctgagtg tgacttccga
     1141 ctcgatcgtc tcgttccgca gcgagagccc tgccagggag acggctcccg cgaagacggc
     1201 gagggccgcg ccgatcgcgg tgaagcggat cgcgcgcaag cgacccccgc gtcggagcac
     1261 gtcggggaga cgatccaccg gcaccgactc gatccggttc gcctcttgct ggagcttcgc
     1321 gccgagcgcc tcatcgcgaa cgtcgctcat cgttccctct cctccaccct cagtgcgtcg
     1381 cgaagcgcgg cccgacctcg ggtcaggtgg acccgcaccg tgctgttctt cgtgccgagc
     1441 atcctcgcga cctcgcccgt ctcgaatccg gcccagtcgc gaagcaccac cgactgccgt
     1501 tgccgaagcg gaaggcctcg aagggcgtcg accacggaca gacgtgcatc agcatcggtt
     1561 ggcctgctcc gctccggaac cgttcccacc acctctctcg ccgcccggcg gcgccgcttc
     1621 gcgcacagcc gcatcgcggt cttgaacacg tagccgccag cgtggtcttc gccgtggatc
     1681 tggccccagt gcgaccagag ccggccgaac gcctcggccg ccacgtcctc cgcgtccccg
     1741 cgatcgagca gcacgagggc gcacgcgcgg accacgcgcg ggaggtgcgc cgcgtagaac
     1801 gcttcgaatc cggctcgatc cggcacgtac ccgttctatc ccgcagcggg gcgacgcgct
     1861 tactcgacgg cgggaggttt tcgtcagccg acgaggtggg agccgaagcg cgcgaagccg
     1921 gcctggcgat cgtgcacggt gacggtcgcg gtgcccagca gctccgcctt ccgccgaagc
     1981 tgctccacca ccttctcgct cggtgagccg tagatgtcga ccgtccgcag gacgaccagg
     2041 tccgcgaacc tcgcgaacac gggcccggcg agctccatgt gacgctccat cgacgcggag
     2101 tcgggatgga tctgcaggac cgtcatgagg tcgccgtcgt caccgaagaa gacctggtac
     2161 gagatcgggt ccgtctcgtt cgtctccacg aaggcggcca gctcagcgat gccacgctcg
     2221 agctccccgc gcttcccctc acggatctcc gacgtgtcga cgacgatgag ggtctccgcc
     2281 atggctcgga ccatagggcg cgcaccacca gcctgcaaga caactacgca ggcgcgagtt
     2341 ccggcacgtc gccgggcggc gcgcagatcg ccccagccga aacgaacgcc acagcgcagg
     2401 gagccaactc cgaccccggc cgggctgccg agaccaccgt ctcgatcgcc gcgagcacga
     2461 tgtcgggagc gtcccgagct cagctcgtac ccgccgacgt cagcggctcc gctcgtcggc
     2521 tcggccgaga cggatgtgcg ggacggcgtc gatgggctcg gcgtgtctcc tgcgccgcac
     2581 gccgccatcg cgatcgaggg cggtcagcgc gatcgtcgcg agccgcccgc atctacagcg
     2641 accgcaggat cgacagcacc gcgcccatca ggggttcccg ggaggcgaac cccgcatgta
     2701 ccgtcgtgcc gttggcgtcc atcaccagtc ctggaagcgt gcctcgtgac acccagcgat
     2761 cgagctccct gctgaactcc tgcgcgtcct tcggcgtgtc ccagaccgtg ctcatgatca
     2821 ccgccacatc gtcgccgtcg gtccaggcgc ggtacgtgcc gccgccccag ccggcggcgg
     2881 ccgacgccgc cacttgatcg tcgagacgga gatggagcag caccttcaac cagagctcgc
     2941 ccacgaccat cacgtccagg tcgcgccagc gcgagccgaa cgtgggtgcg aagtccgcca
     3001 cgtcgaccga ctccccagcc tcgtgcggga acttcgtggg atgcatgacc tgctccgtcg
     3061 tggtggggaa cctctccatc gcgccgtcga tcgcggacgg cccgtccgcg tcggacaggg
     3121 cgtccgcgaa ccgctgcccc gtggtgtacg ggaacgcctg catctcgacg atgaacggcg
     3181 ggacctcgcc ggggtcatca cccgggatcg ggccgacctc ctggatgggg aaccggatca
     3241 acacctgggt cgcgaagtgg ttcgcgctcc cctccacgat gccgagcgcg gcctggaagc
     3301 gctcctcttc gcatcgcgcc acgagaccgt cgaggcggtc gaggtcgaag tgctggtcgt
     3361 cgatcgcgtg cgtgagctcg tgggccagca cgaactgctc gatccggttg agctcggcgt
     3421 cgccggtgta gacgagctcc tcgttctgcg agttgtagaa cccgaggacc tgtccctgtt
     3481 ggtacgcgtc gatcgcctcc aggtatccga cgtcgcgtgg caacgcgccg atcgtctgcc
     3541 atgcctcgct cctgcgagcg tagaaccgct tcgggtagta cgtgccgagg tagtcggcca
     3601 accgctggtc catctcgggt tccgtgatcg gctcgacgtt cacctcccgg tcgaagtcca
     3661 ggccgcgtac cgactccacc tgatcggcga cctccgcgat cgccggcggc gtgggcatct
     3721 ccctcacggg atccacctcg atgtcgggtg cgacgcagag ccggcgcatc gcctcggccg
     3781 ccggggtcgg acccgtcgtg acctccaccg gatcgccgga cggcgtctcc ctcggcgatc
     3841 ccgacggcga cggtggtgac ggctccgacg tgcacgccac gaggagcatc acgatcgtga
     3901 cgccgagccg cctcaccttc gcctcgacac gaccgcgctc accgcggcca cgatcgccag
     3961 tcccgagagc acggccgacg ccgtgaggag cgtcgtggac tcgaggaacc ccgcggtgga
     4021 gacgaccagc gtgacaaccg ccgccaccca gagaacgacc gccgccagat acgcgatcac
     4081 aagcgctccg tcagatagtc gatgaggccg tcgatcgcca agcgatcctg ccccatcgaa
     4141 tcccgttccc ggaccgtgac ctgcgcgtcc tccagggagt cgaagtcgac cgtgacgcag
     4201 aagggcgtcc cgacctcgtc ctgccgccga tagcgacgcc cgatcgaccc cgcgtcgtcg
     4261 aaatcgcaca tccactcgct cttcacgagg tcgaagaccc gccgggcttc gggggcgagc
     4321 ttctcgttgc gcgacagcgg tagcacggct accttcgtcg gcgccagccg ccggtcgagc
     4381 gcgaggtagg tgcgcttctc ggtcccgccc ttcgctgtcg gtgcctcctc ctcacggtag
     4441 gcgtcgagga ggaacgcgag gaccgcccgg tcgacgcccg cggcgggctc gatcacgtag
     4501 gggtggtacc gctcgttctt ctcctggtcg aagtaggtga ggtcctcccc ggaagctgcc
     4561 tggtgcgccc tgaggtcgaa gtcggtccgg ttcgcgatcc cttcgagctc cccccagtcg
     4621 gtgaacggga accggtactc gatgtcgacc gtccgcttcg agtagtgcga gagctcgtcg
     4681 gcgtcgtgct cgcggatccg gacgtgatcc tcacggagcc cgaggtcggt ccaccagcgc
     4741 atccgctcgc cgatccagaa ctcgtgccag tcctcgtcgg tgccgggctc cacgaagaac
     4801 tccatctcca tctgctcgaa ctcccgctga cggaagatga agttccccgg tgtgatctcg
     4861 ttgcggaacg accgaccgat ctgggcgatc ccgaagggga ccttcttgcg gctcacctgt
     4921 tggatcgtgg cgaagtcgac gaacatcccc tgcgccgtct cgggccgcag gtacaccgtg
     4981 ttctcggcgt cctcgatggg gcccatgttc gtcctgaaca tcaggtggaa gttgcggggt
     5041 tgcgtgaaag ccgtctgacc gcagttcggg cacgcggccg gctccgggag ctcatcggcg
     5101 cggaaccgct gatggcagtt caggcactcg accaaggggt ccgtgaagac ctcgaggtgg
     5161 ccggatgcct cccagacctt gggcgacatg atcacggaag catcgagccc gaccacgtcg
     5221 tcgcggagtt gcacggtcga acgccaccac tgctcgcgga cgttgcgctt gatctcggtg
     5281 ccgagcggcc cgtagtccca ggacgagcgg agcccgccgt agatctccga agactggaac
     5341 gcgatccccc gccgtttggc gagagaggtg acggcctcca tcaggtcagg ccttggcagg
     5401 tcaggccttg gcaggtcggc catgaggcgt ggactccttt cccggtcgct ggctgcggcc
     5461 gccggcgtgc cgctcatgct accggggtcg atcgatctgg aggacctacg atcggggcgg
     5521 gccgccgggc gatcccccgg gcgggccctc gtgcggctgc aggcgctcgc cgcccggctt
     5581 cttgcgggcc gccgtccggg ccagggccgc gcggaccgcg ccctgatcga cctcgctcgc
     5641 gtcgtggggc tgccggtcac tctcggtgag gcggatgaac atctcgaggg cggcctcgtg
     5701 cgcctcggcg tccgcgagct ctggatcggc cgccgttggc gcgggtggcg ggctcgcctg
     5761 ggtcgttgcc atcgcggcca cacggctctc gggcaccgag gtgggctccg gcaccaggat
     5821 cgggtcggag gcggcggtcg gctgcgtctt caccggctcc tccttcggcg gctcctcctt
     5881 cggcggctcc tgcttcgccg gcttctccgg cttcggcttc cgcacgcgct tctgtctgtc
     5941 ggccggcggt ctcggagcgc gcgccgtcgc cggcggcggc ttctgatcgc gagacgcgcg
     6001 ggcgagcgga ccggcgggcg atgcggtcca ggcttcatca gacggcatct ccacgacgtc
     6061 ggattccggg acctggacga tggccggttc gggcggggtg tcggccacag gcagtggcga
     6121 gacggccccg ctggaaccgg ctgccttcga cagagcctgc tgcatgccag cttccaggcg
     6181 gcggcgcgcc tcctcggact cctcgagctt ctgctgcaag cgaccgacgc gttccgaggc
     6241 gagacgatag acctcctgga cggcgtgcag ccggtcgttg tcgatcaacg agaagccggc
     6301 gggcacggac gacgaggcga acggcacacc cgtgttgatc ggctcgatcg gatcgcgaag
     6361 cgacaggccg gtcaggacga gggacaggag cagtaccgcg ccggcgagga tctggtagta
     6421 catccactcg gtccgcacgg cggcgagggt gccctcgtcg gtctggtcga tctcggcgat
     6481 cgcgccggcc tcgagcggca cgtaggtccg gacgaggctg cgttccgggt cgtcctcccc
     6541 accggtgtcg gagatacccg agcgggtgag cacaccctcg cgcgcgaccc gtcggaggag
     6601 ttgatcgttc aacccggcgt tggatccggg ggtgtcggcc tggtcggtcg agaacagaag
     6661 cggcccctcg gccgaccaga tgcgaacgcg cgtcagccgt tcgtcagcca ggatcgtgcg
     6721 ctcgacggcc gcctcgagcg acaccgccga ctgacccgtg atgggaccgt cgagagccga
     6781 gccctcgagc ctcggggcga gctcctgctc cacgtaccgc acggctcggc cctgcgcgtt
     6841 cgcgcgctcc cgttcgaggg cgtcgttccc gttcatgatc gcgaacgcga gaccgccgat
     6901 ggccgcgagg gccatcacgg cccatacgta cttgctggcg ccgtctctgg tgaaccgagc
     6961 gatcacgatc cgcccctcct cgccggtgcg ccgctcgcca ccggaagcac ggtcccgtgc
     7021 tctccgggca cgatacagcg ccgagcatct cgtccggaac ggccgaacga ccacggcggg
     7081 ttaggtcgga tggctgaccg aacggaccga acggcggaga cggtcgctca gggttcgtcg
     7141 ccgaccccgc cgaagcgccg cgagcgcttc tggaagtcgc ggatcgcctc gtaaaggatc
     7201 tcgcggtcga agtccggcca gaacaccggc gtgaaccaga gctcggaata ggcgacctcc
     7261 cacaggagga agttcgagat ccgttgctcc ccgctcgtgc ggatcagcag atcggggggc
     7321 ggcgtgtcgg ccgcgtacag gcgctccgcg atcgtggatt cgctgatgcc ctcggggcga
     7381 agcgtccctg cgcggacctc gcgggcgatg tccttcacgg cgtccacgat ctcggcccgc
     7441 cccccgtagt tgaacgcgat cgacagggtg gtcccggtgt tggcggcggt catcgcctcc
     7501 gcggcccgta tctccttcag gacggagcgg gggatacgcc agttgcgccg tccgatgaac
     7561 cggatccgga cgttcatctc gtggagcgcg tcccgccgac gccggagcat ggtgcggttg
     7621 aagttcatca ggtagcgcac ctcgcccgga ggacggcgcc agttctcggt cgagaacgcg
     7681 aacacggtga gccaacggac tcccaccgcg acggctccct ggatcgtgtc ccacagggcg
     7741 tcctcgccgg cgccatggcc ctcggttctg ggcagtccgc gttgctcggc ccaccgaccg
     7801 ttcccgtcca tcacgatccc gacgtgcgcc gggatccggt cgagatcgag gtcctggagg
     7861 tagctgctca cgtcgcagtc cggacgagca tcgggacgga cttcatccgc cgctccaggt
     7921 ggtattcggc gaagccgaac aggaggccgc gcgcttcctt cctcgtgcgc gactccggcg
     7981 cggtcgtctg gcccgcatcc gcgaggtccg cgagcgccag cgccccgagg aacgcgagcg
     8041 ccgccgcggt gacgtggccg gcgccgggct cggcatcggc ggcgcagacg gcgcccccga
     8101 gcccagcgga gaacagctgg acgtcggtgc tgccgcagat agcgcaggcc gtgaggctgg
     8161 ggtggaaccc cgagatactc aagagcttca gcaggaacga ctccgcgacc gcggcgggat
     8221 cgatcggtcg cgcctccagc gaccgcagtc ccgagagcag caggagcacg acacgcacgt
     8281 tccgctcgtg ttcctcggcg acgcggtcga ccgcctcgag catcgtctcg ccggcggcga
     8341 acagcgagag gtcgtcacgg atcgcgcggt ggggggcgat gatctcggcc tgcgtgatgg
     8401 tgtcgagcga ccctcggccg cggtagagca tcaggttcac gtgggtgaac ggctccagac
     8461 gcgccccgaa ccggctggtg gtcttgcgga tccctttcgc cacacctcgg accttgccgg
     8521 aaccctgggt catgatggtg acgatcttgt cggcttcgcc gagcttcgcc gaccggagga
     8581 cgacgccctg ctccttgtag gggggcatct cagaatcgta gcccggtgag aggtcgctcc
     8641 accccacacg gcgtgtgacg tttccgttgc gagaggtgat ggcgccggcc cggaaggccg
     8701 gcgcccaccc ctcacgcctc caggttcttg gtgacgacct ccatcgtctc gttcagctcc
     8761 gccgtcgggc tgaactcgac gatctcggtg tcggggaaga tctccggcgt gtgaccggga
     8821 cccacgtagt aggcctcgcc gtcggtgatc tcgatatcgc cttcgacggt gtggtacacg
     8881 agcttcccct tgaacacgta gccccagtgc ggggactggc actgatcgtt cgggagtccc
     8941 acgaacagcg gagtcatgtc cgaatgctcc gtgtatctct cgaaaccgat cgtgtagccc
     9001 tccgtctctc cgtaccgacc ctcgaaaccc ggtccctcga actgctccgc catggagtcc
     9061 ttcgagacct tgcccatcac ggtctcccct ctactcgtcc tccggtcacg ttcccaaccg
     9121 gtccctgtca gcctctcgaa cgttcaccgt tcctgcgagg gatccggcat ccgttccgcg
     9181 aagactcgag ctcgtaccgc tcgaacgccg gccctcaggc gcccatcgga tgccaaaccg
     9241 tcttgtactc gacgaaggcc gtgacctcgt agggactctg ggcctcttcg tcgaacgggt
     9301 cggcgccggg cgcccgatgc acccgcttca cgttctccgc cgacagctcc tccacctggg
     9361 cccggagctc gtcggggact ccggtggcat cgagcccgtt cacgtccatg tgtccggcga
     9421 gccacgggac gagctcggcg gtctgcccgg tgaggatgtt gacgacaccg cccggcacgt
     9481 ccgaggtggc gagcacctct ccgagcgaca cggccggtag cggggaccgc tcgctggcca
     9541 agacgaccgc cgtgttgccg ctcacgatcg ccggcgcaac gcgcgacacg agcccgagga
     9601 gcgactggtc ctgcggcgcg acgatcccca ccacgccggt gggctccggg atcgtgaagt
     9661 tgaagtacgg acccgccacc gggttcgagg cgccctggat ctggtggatc ttgtcggccc
     9721 accccgcgta ccagatccat cgatcgatcg cggcgctcac gccgcgggcg ggatcttcgg
     9781 cgccggcgtc ccccagctcc tgttcgaact ggacgcgccg gccctccatg agctcggcca
     9841 cccggtacag gatctgggcg cggttgaacg ccgtcttcgc cgcccatccc ggctgggcgg
     9901 cacgcgccgc cctcacggcg tcgcggaggt ccttgcgcga cgcgccggat gcccacgcga
     9961 ggagctcccc gtcggcatcg aacacctcgt acgagcgccc cgactcgctc cgggggaact
    10021 cgccggcgat gaacagcttc gccgtccgcc ggaccggcag gcgctcctcg gtcatgacgc
    10081 accgttccct ggcgccgtcc ctggcgccgg ttcccgaggt gtgagctcga ggtagggcgc
    10141 cagcccgtgc aggccgccct cacgcccgaa gcccgattcc ttgtagccgc cgaacggcga
    10201 ggacggatcg aaccggttga acgtgttcgc ccagacgacg cccgccttca ggcgctcggc
    10261 catccagagg atgcgcgagc ccttctcggt ccacaccccc gccgacagcc cgtacggggt
    10321 gttgttcgcc ttctccacgg cctcctccgg cgtccggaac gtgaggatcg acaacacggg
    10381 accgaagatc tcctcgcgcg cgatccgatg cgactggctg acgcccgtga acagggtggg
    10441 tcggaaccag aagccgcgct cggggaggtc gcacgcgggt tggtacatct cggcgccgtc
    10501 ctcgacgccg gaggccacga gctcctcgat ccgcgtgagc tgggcacgcg agttgatcgc
    10561 tccgatgtcg gtgttcttgt cgagcgggtt ccccactcgg agcgtctgga ggcgtgcctt
    10621 cagcttctcg agcagcgcct cgaagatcgg ctcctgcacc aggagacgcg agcccgcgca
    10681 gcacacgtgc ccctggttga agaagatccc gttcaccacg ccctcgacgg cctggtcgag
    10741 tggcgcgtcc tcgaacacga tgttcgccgc cttgccgccc agctcgagcg tgaggcgctt
    10801 gtgggagcct gcgatcgtgc gccggatctg cttgcccacc tcggtcgagc ccgtgaacgc
    10861 gaccttgtcg acgtcggcgg ccacgagcat cgcgccggtc tcccccgccc ccgtcaggac
    10921 gttgaccgtg cccggtggga gctccgccgc ctggatcacc tcggccagca gcagggccgt
    10981 gagcggggtc gtctccgcgg gcttcaggac ggtcgtgttg ccggtggcga gggccggcgc
    11041 cagcttccac gccgccatca gcatcgggaa gttccagggg atcacctgtc cggccacccc
    11101 gatcggccgc gcgtcgcgtc cggggaacgc gtactcgagc ttgtcagccc acccggcgta
    11161 gtagaagaag tgcgcggcgg cgagcggcag gtcgatgtcg cgggattcct tgatcggctt
    11221 gccgccgttc atcgactcga ggacggcgaa ctcccgagcc cgttcctgga gcgcccgcgc
    11281 gatccggaac aggtacttcg ccctttcgcg accggggagg tcgcgccagc gggactcgaa
    11341 cgcatcccgc gccgcggtca cggcgagctc ggcgtcctcc tggccggcct cgggcacctc
    11401 ggcgaggacc tcttccgtgg acgggtcgat cgtcggcatg tagcggccgc tcttcggctc
    11461 gacgaactcg ccgccgatga acaggccgta gcggtcttgg aggcggacga tgtctttcga
    11521 ttcgggcgcg tccgcgtatc cccacgcggg agtagcgggc gtccgcgcct gcgtcgtgtc
    11581 gagctcgatc atcgtcgtga acaaccgtag ccgacttcgg ccgatatcgg cgccaggcac
    11641 ggcaaccagc gtccagccac aggtggttcc cgaagtaccc acgtcgagat gagcccgacc
    11701 gacgccaggg ttgggtggtt ctccgtctat ccgcccgtcc gaggacggag aacgatcgtc
    11761 atgacgaaga gagccgacgt tcaggaggtg ggtctcatga agcgccgatc atggatcgtg
    11821 gccggatccc tcaccggtct cgtcgcttgt tccgccctcg tcgcactcgc cccggttctc
    11881 gtgcgagatc tccgcggacc ggtctacgga gatctcgcgc tgcccgcgct gctggtcagc
    11941 ggagcagccg gcatcttctt cggtgggatc gtcgcctcgc gcacggtcgg gacgaccccg
    12001 cgacgcatag cgctgctggg cgccgcatgc ggggcgatcc tcggtgcggt ggcgacgggt
    12061 tcggtgttcc tggccgtcga ggtcgcgggc gacgactggc cccacggact cgcagccttc
    12121 gtgaccgggg gcatcatcgg agccgtggtg ggcgggttcc tgggaggcgc catcgggatc
    12181 gtgcaggtcg actcacccgc cgtcgatgag ggtcatgcgc cccgcggtgg ctgatcggcg
    12241 tcgaccgcca cttcccgccg agacgtcaag taggcgccca ggatcacgag caccacgcct
    12301 gtcagggcga acggccccac gacgtcgtcg cggatgtgca ccccgagcag caaggccacc
    12361 acggggatca ggtagccgac gatcgaagcg cgcgtcgctc ccacccgccc cacgagccga
    12421 cccatcagca cgaacgccac gccggtcccg agcatgccga cggcggccac cgcgaggagc
    12481 gcggaccatg cgaac
//
LOCUS       DASUYD010000245        17723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1293578, whole genome shotgun sequence.
ACCESSION   DASUYD010000245 DASUYD010000000
VERSION     DASUYD010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 17723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17723
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1293578"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VF195_12880"
     CDS             <1..517
                     /locus_tag="VF195_12880"
                     /inference="COORDINATES: protein motif:HMM:NF012564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SecY family transport protein"
                     /protein_id="HEX6581750.1"
                     /translation="TYLPLKVNQAGVIPIIFATSVLYFPALLSSVYHAAWFQNFVNNH
                     VQNQRSPVYMFLLAMLIIFFSYFYTAIQFDPTRTADNLRKNGGFIPGIRPGPPTADFL
                     NRILIRIGLPGAIFLAVIALIPTVVFAFWNIQQFPFGGTSILIAVGVALETMKQIESQ
                     LLMRHYEGFLG"
     gene            522..1172
                     /locus_tag="VF195_12885"
     CDS             522..1172
                     /locus_tag="VF195_12885"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015590328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX6581751.1"
                     /translation="MRLVLLGPPGAGKGTQGVLLAERYGIPQISTGDILRDHVQRGTK
                     LGIQARSYMDRGEYVPDGVVVSMVMDRLEEPDADKGFILDGFPRTVAQAMALERALDE
                     AGEPLSSVIKFSVGGEVAVRRLLGRYTCPNCGRTYHAEFKPPAQEGVCDVCGAELERR
                     ADDDELTVRRRIAVYREQASPLEQFYVERDLLYQVDAEGTPDEVIDRTMQVLEGLG"
     gene            1169..1930
                     /gene="map"
                     /locus_tag="VF195_12890"
     CDS             1169..1930
                     /gene="map"
                     /locus_tag="VF195_12890"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEX6581752.1"
                     /translation="MIIRKSADEIEKMRKAGRIVAGTIETVVAAVEPGRSTLDLDRVA
                     ERFIRAQGAIPSFKGYRGTYPASICTSIDAEIVHGIPSGTRKLKEGMVLSLDFGAIWE
                     GFHGDAAVTVFVGGQAPSEEAAKLAKETEHALYAAIDAIRVGGRLSDIGHTVESVADA
                     AGLGVVREYGGHGIGRSMHEEPFIQNWGPPGRGLELRPGLTIAVEPMFTLGSEENRVL
                     ADGWTVVTTDGSIAAHFEHTIAITEDGPEILTARS"
     gene            2045..2263
                     /gene="infA"
                     /locus_tag="VF195_12895"
     CDS             2045..2263
                     /gene="infA"
                     /locus_tag="VF195_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEX6581753.1"
                     /translation="MPKDDVIEVEGTVVEPLPNAMFRVELENGHKVLAHISGKMRMHY
                     IRILPGDRVKVELSPYDLTRGRVVYRFK"
     gene            2299..2412
                     /gene="rpmJ"
                     /locus_tag="VF195_12900"
     CDS             2299..2412
                     /gene="rpmJ"
                     /locus_tag="VF195_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006042225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEX6581754.1"
                     /translation="MKVRPSVKKMCDRCRVIRRRGVVMVICTNPRHKQRQG"
     gene            2426..2809
                     /gene="rpsM"
                     /locus_tag="VF195_12905"
     CDS             2426..2809
                     /gene="rpsM"
                     /locus_tag="VF195_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017986631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEX6581755.1"
                     /translation="MARIAGVDLPRDKRLDIALRYIYGIGSTRAYEIVSGSGVEGATK
                     VRDLSEDEVLKIRAYIDQNVKVEGDLRREVAQDIKRKIEIGCYQGVRHRRSLPVRGQR
                     THTNARTRKGPKKTIAGRKKAQAKK"
     gene            2857..3249
                     /gene="rpsK"
                     /locus_tag="VF195_12910"
     CDS             2857..3249
                     /gene="rpsK"
                     /locus_tag="VF195_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013830211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEX6581756.1"
                     /translation="MAKPKAKKARRREKKNVVHGQAHIKSTFNNTIVTITDLQGNTLS
                     WASAGNVGFKGSRKSTPFAAQLAAEKAARQAQEHGVQKVDVFVKGPGSGRETAIRSLA
                     ATGLEISVIQDVTPVPHNGCRPRKRRRV"
     gene            3339..4262
                     /locus_tag="VF195_12915"
                     /pseudo
     CDS             3339..4262
                     /locus_tag="VF195_12915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009740501.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
     gene            4274..4624
                     /gene="rplQ"
                     /locus_tag="VF195_12920"
                     /pseudo
     CDS             4274..4624
                     /gene="rplQ"
                     /locus_tag="VF195_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019610101.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            4733..5479
                     /gene="truA"
                     /locus_tag="VF195_12925"
     CDS             4733..5479
                     /gene="truA"
                     /locus_tag="VF195_12925"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEX6581757.1"
                     /translation="MSVMVLTLAYDGTAFRGWAKQRDPSIRTIEGELVRHLERVLREP
                     VKLSVAGRTDAGVHARGQVASLRTETSVTPERLQKALNSALAPEVVVVRARLAPDGFD
                     ARFSATARGYVYRIDEASLADPFTGRFAWHRPGRLSLAPMRRAARDLEGKHDFRSFCR
                     HPGGDRSTSRHLRRLTVVRAGDRLAIRAEADAFLHQMVRSLVGTLVAVGEGKQDPTAM
                     PSVLAARDRSATSGGLAPAHGLTLERVSYR"
     gene            5574..5981
                     /gene="rplM"
                     /locus_tag="VF195_12930"
                     /pseudo
     CDS             5574..5981
                     /gene="rplM"
                     /locus_tag="VF195_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011293037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
     gene            6377..6772
                     /gene="rpsI"
                     /locus_tag="VF195_12935"
     CDS             6377..6772
                     /gene="rpsI"
                     /locus_tag="VF195_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013016205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX6581758.1"
                     /translation="MATTPTTLTTGRRKAAIARVRLVPGTGEFRINDRSLDEYFPTRV
                     HRMVAQSPLRSVGRDKDFDVVATITGGGVNGQAGALRLGIARALLELEPDLRAQLKAE
                     GFLRRDAREKERRKYGLKKARKAPQYSKR"
     gene            complement(6769..7041)
                     /locus_tag="VF195_12940"
     CDS             complement(6769..7041)
                     /locus_tag="VF195_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581759.1"
                     /translation="MKLVRVFESLDPIRGMLVRGLLEAEGLEVLAKGEGLGPYRTGPV
                     ILFVIEDASGRARELIAAAEDGSLALGSDEQSSGWEGGLAVERSAD"
     gene            7144..7602
                     /locus_tag="VF195_12945"
     CDS             7144..7602
                     /locus_tag="VF195_12945"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEX6581760.1"
                     /translation="MSESLPGVLFVCVHNAGRSQMAAALLERHAAGRVRVRSAGSEPA
                     DRLHPSVVEVMGEVGIDLARERPKLLRDEAVRDSDVVITMGCGDACPVFPGIRYEDWD
                     LEDPSGKPLEDVRRIRDDIDGRVRRLLEALTGQAESAERSEASGSGRTRK"
     gene            complement(7545..7877)
                     /locus_tag="VF195_12950"
     CDS             complement(7545..7877)
                     /locus_tag="VF195_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX6581761.1"
                     /translation="MKLVQDLDACCTPLLSSPLSEDAAETLATALQALADPARLRLLS
                     LIAACPGSEACGCDLTTPIGLSQPTVSHHLKVLHEAGLLERERRGKWVYFRVRPEPLA
                     SLRSALSA"
     gene            8073..9404
                     /gene="glmM"
                     /locus_tag="VF195_12955"
     CDS             8073..9404
                     /gene="glmM"
                     /locus_tag="VF195_12955"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX6581762.1"
                     /translation="MARLFGTDGVRGIAGSELTASLARGLGRAAVRVLATGVEHRPVL
                     VLGRDPRESGTWLEEALVGGVHEAGGDVLLAGVEPTPAIAFLTIDVGADAGVVISASH
                     NPPEYNGIKFFGPNGMKLPDVVEDRIEAGLSDTSSHVAGVGSIQDLAGGRERYLAHLE
                     AAAGARLDGMTIVVDCANGAASGLAPTLYERLGATVHPIHDAPDGRNINVDCGALHPE
                     VAGAAVVELGADAGVSHDGDADRALFVDAAGTPIDGDQILAACAIAMKEAGALPSNAV
                     VTTVMANLGFHRAMRDAGITVSATKVGDRYVLEEMLEEGVMLGGEQSGHLIFRDHATT
                     GDGLLTAVRFLSLAAGSGRSVADLAGVMQRYPQVMENVQVRSLTQLDSSEHVWEAVRA
                     AEEELADHGRVIVRASGTEPLVRVMVEAETEDVARRLADAIAGEVRKHLGA"
     gene            9470..11302
                     /gene="glmS"
                     /locus_tag="VF195_12960"
     CDS             9470..11302
                     /gene="glmS"
                     /locus_tag="VF195_12960"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016575940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX6581763.1"
                     /translation="MCGIVGYVGPDEALPIVLEGLRRLEYRGYDSAGVAVLEGDLTVV
                     KRAGKLSELVTALDDGGHPGGSVAMGHTRWATHGAPTHRNAHPHLDCSGTVAVIHNGI
                     IENFQQLRARLEKEGHTLASETDTELVAHLIEERLRYGGSLVEAVRATVRELEGAYSL
                     VVMSSEEPGLLIGVKVASPLVVGVGSGESILASDIPAVLGRTTTVIPVDEGRIVEVRA
                     DGATFSDFDGTVVHPESIQVDWDAARAEKGGFDTFMLKEIHEQPSAIRDTLVGRVDEM
                     RRLALDDLRISDDVLREVDKVFVVACGTAYHSGLVAKYAIEHWTRLPVEIDIASEFRY
                     RDPVLGPNTLTLAVSQSGETIDTLEAARHARRQASPLLAVTNTVGSSLAREADGVIYM
                     HAGPEIGVAATKTFATQMVSQYILAMYLAQVRGSMFPEEIAEVLTRMGELPAQVERAI
                     GLDAQVQELARRFQDSRDWLFIGRHTGYPAALEGALKLKEISYVHAEGYPAGELKHGP
                     IALVEDGVPVVAVATTCHVYPKMLSNIQEVRARGAEVIAVATEGDEQIRGVAQHVLEV
                     PETPELLSPVVVTVPLQLLAYHVARLRGSDVDQPRNLAKSVTVE"
     gene            11312..11875
                     /locus_tag="VF195_12965"
     CDS             11312..11875
                     /locus_tag="VF195_12965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581764.1"
                     /translation="MARVMARPRALRLMLLAVLVAGLLVPGAPARACSCAEMDLGSLL
                     PDADGAFVGTFVDRSEISDQLASITFDVERVVKGEFGPRAIVRTNAYGASCGLEFLDG
                     PRIGLLLDRASDGVWESSLCQQVQPGDLLAIGGERPPDPDVAPVSAGWTLGSQGLVGT
                     IVAAALVLVVLVWVIRRRSSRPGSGLT"
     gene            11886..12257
                     /gene="acpS"
                     /locus_tag="VF195_12970"
     CDS             11886..12257
                     /gene="acpS"
                     /locus_tag="VF195_12970"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEX6581765.1"
                     /translation="MDIAGLGVDLCEIARMERAISRHPTFRDRVFTPEEIAYCDSKAR
                     PAESYAGRFSAREATIKALGGYRGRRWQDISVTRHPSGAPAIRLDGNAKRRADALGVT
                     QVLITFTHERTNAVAFAIAVR"
     gene            12254..13786
                     /locus_tag="VF195_12975"
     CDS             12254..13786
                     /locus_tag="VF195_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEX6581766.1"
                     /translation="MKPVLTPEEAAALDRDTQARGIGAEVLMERAGRAVARAARELAG
                     GSYGRRAVVVCGKGNNGGDGLVAARHLAREGVRTIVVLLEDPDELRDPAASNARRLAE
                     VPGIRVRTFDEHALRRELVRADIVVDAIFGTGFRGMPEDDWASAIESLNEADAPVVAV
                     DIPSGVDGATGVVEGEAVRADLTVAFGAPKLGTVLMPGAEHAGILRVVDIGFPDDLLR
                     ARAWLTEPDDVAGWLPQRDIDTHKRASGVLVVVAGSRAMTGAARLIAMSAGRVGTGLV
                     VVAAPEDCLSQIQAGLTEPTFLPLPETADGTVAAEAAAPVLERLEAADALAIGPGLTT
                     DQATVAFVRDVVRRSPVPLVLDADGLNAFTADATALEDRASEAVLTPHVGEFGRLTGV
                     KARDLDADRPAHVRALADQTGAVALLKGSRSVIANPEGRLFVNVTGSPTLATAGTGDV
                     LTGMIGGLLARGVPPTESAAAAAYLHGLAGLFAGRDLGEGAIAGDVMDRVPEAVAHVE
                     GK"
     gene            13827..14924
                     /gene="alr"
                     /locus_tag="VF195_12980"
     CDS             13827..14924
                     /gene="alr"
                     /locus_tag="VF195_12980"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX6581767.1"
                     /translation="MSESYRPTWVEVDLEAIRHNARVLKPETAELMAVVKANGYGHGD
                     VEVARAAIDAGATWCGVALVEEGLRLRSGGIEAPILVLSELPAGSEAVALAHRLTPTL
                     YSDGALERLASATRGSVPVHVKVDTGMHRVGVWPPEDAPAFARRVQEAGLEVEGLFTH
                     FARSEEDEATTKEQLERFLDTADAARTAGVRPRVLHSANSGATILHPESHLDLVRPGI
                     ALYGIEPAPGIGAHLGLRPALTWRSRVSAVKRLPAGEAVSYGHRYRLEHDAWLATVPV
                     GYADGYPRQLTNLGSVVIGGRRHGIAGTVTMDQLLVDCADEEIQVGEEVVLIGNQGDE
                     TIGAEELGRLIGTIGYEIVSRIGDRVPRRYG"
     gene            14921..15991
                     /locus_tag="VF195_12985"
     CDS             14921..15991
                     /locus_tag="VF195_12985"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6581768.1"
                     /translation="MRKVVSNAPRVAVGAAAAAGVVAAGLAGRALWRHRRPAGGDRAW
                     GPPPEDLGPIEAFDGAELVVRAAGPPDGSILLFSHGFSLDMTTWREQWSDLGDEYRCI
                     ALDHRAHGRSEVPGTGDVSLRSMGRDLATVLDAVAPDRRVVLIGHSMGAMAILALAEQ
                     RPDLFGPRIGGIVLLGASASDLLRGAMGGVTDLLRPRLGSLAAAARRVDRLRRAILAG
                     PAEVPAIVARVTQFAPEAPDDVVDHVLHLAEQTSSLVWTDGLAELMRMDFRHALARIT
                     VPALVAVGDQDRVTPPAVAVELAAALPDGRLFLVEGAGHIAMLERPERLNPELRAFAR
                     EALSFGSDRGRKRRGTRKNGAA"
     gene            15988..16686
                     /locus_tag="VF195_12990"
     CDS             15988..16686
                     /locus_tag="VF195_12990"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013980851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX6581769.1"
                     /translation="MSRTLADIGAEAAGCTRCRLAEGRTQVVFGVGNPDADVLFIGEA
                     PGFHEDKQGEPFVGAAGQLLTKVLGDVLGLDRGDVYICNILKCRPPGNRDPQEDEIES
                     CTPWLVEQISSIQPRVIVTLGNFATKFVLQTQQGITRMRGKAYPWHGRTVIPTFHPAA
                     ILHGGGEKSRQFGELREDFELVRDTLAAMDPGPGSAPAPESSPAPVQVPAPATVRVPD
                     AGVAPVPEEQLELF"
     gene            16689..17210
                     /gene="tsaE"
                     /locus_tag="VF195_12995"
     CDS             16689..17210
                     /gene="tsaE"
                     /locus_tag="VF195_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX6581770.1"
                     /translation="MRLELATAAADGTRDVGAALATLLLPRDTVILTGDLGAGKTTLV
                     QGIGRGLGVEDHVASPTFTLVKEYAGSLDVAHVDVYRLERVQDVVDLGLDELGGPDRV
                     LLVEWGDAVQELLPEDRLRVELTTGSDDDARRIVLTPHGRSWALRWERLEQLLEPFRR
                     DGPSGGPVAEAAP"
     gene            17207..>17723
                     /gene="tsaB"
                     /locus_tag="VF195_13000"
     CDS             17207..>17723
                     /gene="tsaB"
                     /locus_tag="VF195_13000"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEX6581771.1"
                     /translation="MIVVGIETSTPQTSVAIGSERELLGAVSVAGKARQEAVTPALER
                     LCGWTGVELSQVGGIAVGIGPGLFTGLRVGIETAKTLAQVLSVPIVGITSLDALAFSV
                     RHTHKLIVAVIDGRRREVFFAIYRALPGGVVRGSEYTVATPDHLCAELETVPGEMLAV
                     GDGAILYRNQLE"
BASE COUNT         2819 a         5850 c         6419 g         2635 t
ORIGIN      
        1 cacgtatctc ccgctgaagg tgaaccaggc cggcgtgatc ccgatcatct tcgccacgtc
       61 ggtcctctac ttccccgccc tgctgagcag cgtgtaccac gccgcgtggt tccagaactt
      121 cgtgaacaac cacgtccaga atcaacggag ccccgtctac atgttcctgt tggccatgct
      181 gatcatcttc ttctcgtact tctacacggc gatccagttc gacccgaccc gcacggcgga
      241 caacctccgg aagaacggcg gattcatccc cggcatccgg ccgggaccgc cgaccgccga
      301 cttcttgaac cgcatcctca tccggatcgg gttgccgggg gcgattttcc tcgccgtgat
      361 cgcgctgatc ccgacggtcg tgttcgcgtt ctggaacatc cagcagttcc cgttcggcgg
      421 tacgtcgatc ctgatcgccg tgggggtcgc cctcgagacc atgaagcaga tcgagtcgca
      481 gctgctgatg cgtcactacg agggtttcct cggatagtcc gatgcggctc gtgctgctcg
      541 gccccccggg cgcggggaag gggacacagg gcgtcctgct cgccgagcgc tacggcatcc
      601 cccagatctc gaccggcgac atcctgcgcg accacgtgca gcgggggacg aagctcggca
      661 tccaggcacg ttcgtacatg gatcgaggcg agtacgtgcc ggacggcgtg gtcgtatcga
      721 tggtgatgga ccgcctggag gagccggacg ccgacaaggg attcatcctc gatgggttcc
      781 cacgaaccgt cgcgcaggcg atggcgctgg agcgggcgct cgacgaagcc ggcgagcccc
      841 tctcctcggt gatcaagttc tcggtcggtg gtgaggtcgc cgtgcggcgg ctcctgggcc
      901 gctacacgtg tcccaactgt ggacggacgt accatgccga gttcaaacca cccgcccagg
      961 aaggggtctg cgatgtctgc ggcgccgagc tcgagcgtcg ggccgacgat gacgaactga
     1021 ccgtgcggcg ccggatcgcc gtgtaccggg aacaagcttc gccgctcgag cagttctacg
     1081 tggagcgcga cctgctttac caggtggatg ccgaaggcac gccggacgaa gtgatcgatc
     1141 gaaccatgca ggtcttggag ggtctcggat gatcatccgg aaatcggccg acgagatcga
     1201 gaagatgcgg aaggcagggc ggatcgtggc cggcacgatc gaaacggtgg tggctgcggt
     1261 cgagcccggg cgttccacgc tcgatctcga ccgcgtggcg gagcgcttca tccgggcaca
     1321 gggagcgatc ccttccttca aggggtaccg ggggacctac cccgcgtcga tctgcacctc
     1381 gatcgacgcc gagatcgtcc atgggatccc gtcggggacg aggaagctca aggaaggcat
     1441 ggtgctctcc ctggacttcg gggcgatctg ggagggattc cacggtgacg ccgccgtgac
     1501 ggtgttcgtg ggcggccagg cgccatccga ggaggcggcg aagctcgcga aggagaccga
     1561 gcatgcgctc tacgcggcga tcgacgcgat ccgtgtcggc ggcaggctct cggacatcgg
     1621 ccacaccgtc gagtcggtcg ccgatgctgc gggattgggt gtggtccggg aatacggcgg
     1681 ccacggcatc ggccggagca tgcacgagga gccgttcatc cagaactggg gtcctccggg
     1741 acggggtctg gagcttcggc cgggactcac gatcgcggtg gagcccatgt tcaccctcgg
     1801 aagcgaggag aacagggttc tcgccgacgg gtggacggtg gtgaccacgg atgggtcgat
     1861 cgcagctcac ttcgagcaca cgatcgccat caccgaggac ggccccgaga tcctgaccgc
     1921 tcggagctga atccccggtc tgcaggtata cttcctcgtt cgccccttct ggaggccgtg
     1981 ttcgcatgtc cggccagcag gggtcgagag caggaaagcc ccgttcgaag gaatcagtgg
     2041 gagcgtgccg aaagacgacg tcatcgaggt cgaggggacc gttgtcgagc ccctgccgaa
     2101 cgccatgttc cgggtggaac tggagaacgg gcacaaggtg ttggcccaca tctcggggaa
     2161 gatgcggatg cactacatcc ggatcctccc cggggaccgg gtgaaggtcg agctctcgcc
     2221 gtacgacctc acacgtgggc gagtggtgta taggttcaag tagcagaaga tgaggaccag
     2281 ggaagcgagt agaagcagat gaaggtcagg ccgtccgtga agaagatgtg cgaccggtgc
     2341 cgggtcatcc ggcgccgggg cgtcgtgatg gtcatctgca ccaacccacg gcacaagcag
     2401 cggcagggat aggaggctcg aagggatggc acggatcgca ggcgtggatc tccctcgcga
     2461 caagcggctc gacatcgcgc tccggtacat ctacggcatc ggctccacgc gtgcctacga
     2521 gatcgtgtcg ggttccggtg tggagggcgc gaccaaggtc cgcgatctct ccgaggacga
     2581 ggtcctgaag atccgcgctt acatcgatca gaacgtgaag gtcgagggag acctgcgccg
     2641 cgaggtggcg caggacatca agcgcaagat cgagatcggc tgctaccagg gtgtccggca
     2701 ccgccgatcg ctcccggtcc gtgggcagcg cacgcacacg aacgcccgca cgcggaaggg
     2761 accgaagaag accatcgcgg gcaggaagaa ggcccaggcg aagaagtagc gcgaggcgcg
     2821 acgaggatcg ggtcaaggag cacgagaaag ggatcgatgg cgaagcccaa ggcgaagaag
     2881 gcgcggcggc gggagaagaa gaacgtcgtc cacggtcagg cgcacatcaa gtccacgttc
     2941 aacaacacga tcgtcaccat cacggatctc cagggcaaca cgctctcgtg ggcgagcgcg
     3001 ggcaacgtgg gcttcaaggg ctcccgcaag tccacgccct tcgccgcgca gctggccgcc
     3061 gagaaggccg ctcgccaggc ccaagagcac ggcgtccaga aggtcgacgt gttcgtgaag
     3121 gggccggggt cgggccgcga gacggccatc agatccctcg ccgccacggg cctcgagatc
     3181 tccgtgatcc aagatgtcac gccggtcccg cacaacgggt gccggcctcg caagcgtcgg
     3241 cgcgtctagc gaccggcgag cccggcaccg agggggtgtc gggacgcacc acgacgcggg
     3301 gaaggaatgg ttgatccccc gccggaggag gaagcaccgt gttcatcgtc gccagacccg
     3361 agatctccga gaacgagatc tcacccatcc gctcaaagtt cacgatcgag ccgctcgaac
     3421 cggggttcgg ctacacggtc ggcaactcgc tccgccggac gctgctgtcg tcgatcccgg
     3481 gagccgcgat ctcgtcagtc cgcatcgacg gtgtgctgca tgagttctcg acgatcccca
     3541 aggtcaacga ggacgtcacc gacatcatct tgaacctcaa ggagctcgtg atccgctcgg
     3601 atctcgagga gcccaccacc gcctatctga agtccaaggg tccggccgag gtcacggcgg
     3661 gcgacatcgc accgccggcg ggggtcgaga tcttgaaccc cgacctccac gtcgccacgc
     3721 tcggcaaggg cgcgtccctc gagatggagc tcaccgtcga gcgcggcgtc ggttaccgga
     3781 tggccgacaa gaacaagaag gcacgtgacc cgatcggcgt gatccccgtg gactcgatct
     3841 tctcgcccgt ccggaaggtg tcgtacgcgg tcgaggacac ccgagtcgag cagatgacgg
     3901 accgcgaccg gctgatcctc gacgtggaga cggacggctc cgtcacgccg cgcgaggcgc
     3961 tcgcgtcggc gggcgggacg ctcctcgagc tcatccagct cttctcggac ctcgccgagg
     4021 cggcgagcgt ctccgtcggg ccggccgagg acgaggacca gccgagcgac tacgcgatca
     4081 cggtcgagga cctgaatctg tcggtgcggt catacaactg cctgaagcgc gagggcatca
     4141 actcggtggg cgacctggtc cagaagtccg agtccgagct catggacatc cggaacttcg
     4201 gccagaagtc gatcgatgag gtcaaggcca agctcgagga gctcggcctc ggcctgcggg
     4261 aggagtaggc gccgtgccca agcccaagaa gggcccccga ctcggttcca gcccggctca
     4321 ccagaagctc ttgctgtcca cgctggccgc ccagctcttc gtgcacgagg ggatccgtac
     4381 gaccgaggcg aaggcgaaac agctccgtcc gttcgcggag aagctgatca cgttcgccaa
     4441 gcggggtgac ctcgcggcgc ggcgcgaggt gcttcgcgac atccccgacc gcgacgtggt
     4501 cgcgaagctg ttccacgaga tcggccctcg gttcgaggat cgaccgggcg gctacacgag
     4561 gatcctgaag ctcggcccgc gcaagggcga cggggccccg atggccctga tcgagctcgt
     4621 ggagcgcgag gaggtctagc gtcgtggccg ggcggcccgc gcgggggcgc cgcgtgacgt
     4681 gatcgtccgg gggatcgagg atcccgagcc cgatgaacgg aggccgatat ccatgtcggt
     4741 catggtgctc acgcttgcct acgacggcac cgctttccga ggctgggcga agcagcggga
     4801 tccgtcgatc aggacgatcg agggcgagct cgtgcgccac ctcgaacggg tactccggga
     4861 gccggtgaaa ctctcggtgg cgggacgtac cgacgcgggt gtgcacgcgc gtggtcaggt
     4921 cgcatcgttg cggacggaga cctcggtcac gcccgagcgg ctccagaagg cgctgaactc
     4981 ggcgctcgca cccgaggtgg tcgtcgtccg tgctcggctc gcaccggacg gattcgatgc
     5041 ccgcttctcc gcgaccgccc ggggatacgt gtaccggatc gacgaggcct ctctcgcgga
     5101 cccgttcacg ggccgattcg cttggcatcg ccccggccgg ctctcgctcg ctccgatgcg
     5161 acgggcggcg agggatctcg aggggaagca cgatttcagg agcttctgcc ggcatccggg
     5221 aggcgatcga tcgaccagca ggcatctgcg gcggctgaca gtcgtgcgcg ccggcgatcg
     5281 cctcgccatc cgggccgaag cggatgcttt cctgcaccag atggtcaggt ccctggtcgg
     5341 cacgctggtg gccgtgggag agggcaaaca ggaccccacc gcgatgccga gtgtgctggc
     5401 tgcgcgggat cggtccgcca cgtcgggcgg gctggcgccc gctcacggcc tcacgttgga
     5461 gcgggtcagc tatcggtgag cgaaacggtg tgcctgcctg ggaccgagct ttgacgacga
     5521 tgcgtttccg caggtagcat gggccgtcgg cccctcgggc cgacatctct ccgatgaaga
     5581 cctactctcc caagcctgag cacatcgaac gccgctggta cgtcatcgac gccggcgagc
     5641 aggtgcttgg gcgtgttgcc acggaggccg cggtcgttct ccgcggcaag cacaagccga
     5701 tcttcgcccc acacatggac gtcggcgaca atctgatcat catcaatgcg gcgaaggtgg
     5761 tgctcacggg cggcaaggag acgaagaagg tggcctggcg tcactccggg catcccggtg
     5821 gcatcacggg caccccgtac gaggatctcc tcgagaagcg gccggcgttc gtggtggaga
     5881 aggccgtccg cgggatgctt ccgaagaacc ggctcggtcg cgcgatgatc aagaagctcc
     5941 acgtggttcc tgggccggag cacaaccatc aggcacagaa gccccaggag tggacgatgg
     6001 ggatgcggcc cgtgtgggag ggcctgccca caccgcccgc ctcggtagcg accgagaaga
     6061 aggccgcgcc gaagggcgaa acggctgagc taccggccgc cgcgaagcgg acagcggcgg
     6121 cgccgaagga gccgaaggag ccggcggcca cagcgtcgtc gcagaaggcc gcggccacgt
     6181 cggcggggac cagaaccacg gcgaggaaga cggcggcgaa gaagtcgacc accgcgaagt
     6241 cgaccaccgc gaagtcgacc accgccaaga cctcggcgag gaagtcgacc gccaccaaga
     6301 agccgaccgc gaggaagtcc accacggcga ggaagcggac cgccgcgaag acgacggcga
     6361 agaagaagga gacgtagtgg cgaccacccc gacgaccctc accaccggac ggcgcaaggc
     6421 ggcgatcgcc cgagtccgcc tggtgcccgg tacgggcgag ttccggatca acgaccgttc
     6481 gctcgatgaa tacttcccca cgcgagtcca caggatggtg gcgcaatcac cgctccggtc
     6541 cgtcggccgc gacaaggact tcgacgtcgt cgccacgatc accggtggtg gcgtcaacgg
     6601 acaggcgggg gctctccgcc tcggcatcgc ccgcgcgctc ctcgagctcg agccggacct
     6661 ccgggcgcag ctcaaggccg aggggttcct gcgccgcgac gcccgggaga aggagcgtcg
     6721 caagtacggg ctgaagaagg ctcggaaggc tccgcagtac tcgaagcgct agtcggcgga
     6781 gcgctcgacc gcgagtccgc cttcccagcc cgaggactgc tcatcggatc ccagcgcgag
     6841 cgatccatcc tccgcggctg cgatgagctc cctcgctcga ccggacgcgt cctcgatcac
     6901 gaacaggatc acggggcccg tccgataggg gccgagcccc tcccccttgg cgagcacctc
     6961 gagtccctcg gcctcgagga ggccgcgcac gagcatcccc cggatcgggt cgagcgactc
     7021 gaacacgcgg accagcttca ccggcccatc atcgcccgga ccggcgtcaa cgtccacgga
     7081 cgttcaggta tgggtcccgc accgttcacc gtcgatgccg ggcgagcgcg taccctggct
     7141 cggatgagtg agtctctgcc gggcgtcctg ttcgtgtgcg tccacaacgc ggggcggagc
     7201 cagatggccg ccgccctgct cgaacggcat gccgccggcc gtgtccgggt ccggtcggcc
     7261 ggctccgagc cggccgaccg tctgcatccc tccgtcgtcg aggtcatggg cgaggtcggg
     7321 atcgatcttg cccgagagcg gccgaagctc ctgcgagacg aggccgtgcg cgactccgat
     7381 gtcgtgatca cgatggggtg cggagacgcg tgtcccgtct tccccggtat ccgctacgag
     7441 gactgggatc tcgaggaccc ctcggggaag ccgctcgagg acgtccgacg gatccgcgac
     7501 gacatcgacg ggcgggtccg gcgtctgctg gaggcgctga cgggtcaggc ggagagcgcc
     7561 gagcggagcg aggcgagcgg ctccggacgc acccggaagt agacccactt cccacggcgc
     7621 tcccgctcga ggagcccggc ctcgtgcagg accttcaggt ggtggctcac cgtcggctgc
     7681 gagaggccga tcggcgtcgt caggtcgcat ccgcatgcct ccgagccggg gcacgcggcg
     7741 atcaggctca ggaggcgcag cctggccggg tccgcgagcg cctggagggc cgtggccagc
     7801 gtctcggccg cgtcctcact caggggtgac gagagcagag gcgtgcagca ggcatcgagg
     7861 tcctgaacca gcttcatgct ggtcatattg acagatgtcg atgcgtgagg caacatatcg
     7921 accgccatcg atgcgatggc cggagtcgat gggaggtgac ccggatggat ccggactgct
     7981 gcccgcccgg ctgctgctag gcacgtgaga ggtggcgggg cgcggggccg gaggcaccgc
     8041 gtcccgccac ccgcagtagc atcacctgcc cgatggcgcg cctcttcggg accgacggcg
     8101 tccgagggat cgccggctcc gagctcaccg cctcgctcgc acgagggctc ggccgggcgg
     8161 ccgtccgcgt gctggccacc ggtgtcgagc accggcccgt gctcgtgctc gggagggatc
     8221 cacgggagtc ggggacgtgg ctcgaagaag cactcgtcgg gggggtccac gaggccggtg
     8281 gagacgtgct cctggcaggg gtggagccca cgcccgccat cgcgttcctg acgatcgacg
     8341 tgggagcgga cgccggtgtg gtcatctcgg cgtcacacaa tcctcccgag tacaacggca
     8401 tcaagttctt cggaccgaac ggtatgaagc tcccggatgt cgtcgaggac cggatcgagg
     8461 ccgggctgtc ggacacgtct tcgcacgtcg ccggggtggg gtcgatccag gacctcgccg
     8521 gcggccgcga acgatacctg gcacacctcg aggccgcggc cggtgcgcgg ctcgacggga
     8581 tgacgatcgt cgtcgattgt gcgaacggcg ccgcgtccgg cttggccccg acgctctacg
     8641 agcggctcgg ggcaacggtg caccccatcc acgacgcccc cgatggacgc aacatcaacg
     8701 tcgactgtgg cgcactccac cccgaggttg cgggagcggc tgtggtggag ctgggcgcgg
     8761 acgccggcgt ctcccacgac ggagacgccg accgggccct gttcgtcgat gcggcgggaa
     8821 cgccgatcga cggtgatcag atcctcgccg cgtgcgcgat cgcgatgaaa gaggccggtg
     8881 cccttccctc caacgccgtg gtcaccaccg tgatggccaa cctcggcttc catcgagcca
     8941 tgcgggacgc agggatcacg gtctccgcca cgaaggtggg cgaccgctac gtcctcgagg
     9001 agatgctcga ggagggggtg atgctgggtg gggagcagtc gggccacctg atcttccgtg
     9061 accacgcgac gaccggcgac ggcctcttga ccgcggtccg gttcctctcc ctcgctgccg
     9121 gcagcggtcg ctcggtcgcc gatctcgccg gcgtgatgca gcgctacccg caggtgatgg
     9181 agaacgtcca ggtgcgaagc ctcacccagc tcgacagctc cgaacacgtc tgggaggcgg
     9241 tccgagcggc ggaggaggag ctcgccgacc atggccgcgt gatcgtccgc gcgtcgggaa
     9301 ccgaaccgct cgtccgggtg atggtcgagg cggagacgga ggacgtcgcg cgccgccttg
     9361 cggatgccat cgccggcgag gtgaggaagc atctcggcgc ctgagcgccg ttctcggagg
     9421 cccgcttctc gttctcgcgg ccgaaccgcc ctgctaccct cgatgctcca tgtgcggcat
     9481 cgtcggctac gtggggcccg acgaggccct cccgatcgtc ctcgagggac ttcgacgtct
     9541 ggagtaccgc gggtacgact cggcgggcgt cgccgtgctc gagggcgacc tcacagtcgt
     9601 caagcgggcg gggaagctct cggagctcgt gacggcgctc gacgacggcg ggcacccggg
     9661 cgggagcgtc gcgatgggcc acacgcgctg ggcgacacac ggcgcgccga cccatcgcaa
     9721 cgcccacccg cacctcgatt gctccggcac ggtcgccgtg atccacaacg gcatcatcga
     9781 gaacttccag cagctccgag cacgcctcga gaaggaaggg cacaccctcg cgtccgagac
     9841 cgacaccgaa ctcgtggcgc atctgatcga ggagcgcctc cggtacggcg gatcgctggt
     9901 ggaggccgtc cgcgcgacgg ttcgggagct cgaaggggcg tactcgctcg tcgtcatgtc
     9961 gtcggaggag ccgggtctcc tcatcggcgt gaaggtcgcg tcgccactcg tggtgggggt
    10021 cggttccggt gagagcatcc tcgcctcgga catcccagcg gtgctcggac ggacgacgac
    10081 ggtgatcccc gtggacgagg gacggatcgt cgaagtgcga gcggacgggg cgacgttctc
    10141 cgacttcgac ggcaccgtcg tgcatccgga gtccatccag gtcgactggg acgccgctcg
    10201 ggcggagaag ggcggcttcg acacgttcat gctcaaggag atccacgagc aaccgagcgc
    10261 gatccgcgac acgctggtgg ggcgggtcga cgagatgcga cgcctcgcgc tcgacgacct
    10321 gaggatctcc gacgacgtcc tgcgggaggt ggacaaggtc ttcgtggtcg cgtgcgggac
    10381 ggcgtaccac tcgggcctcg tggcgaagta cgcgatcgag cactggacgc ggctgcccgt
    10441 ggagatcgat atcgccagcg agttccgcta tcgcgacccg gtcctcggcc ccaacacgct
    10501 caccctcgcg gtctcacagt ccggggagac gatcgacacg ctcgaggccg cccggcatgc
    10561 gcggcgccag gcatcgcccc tcctggccgt cacgaacacg gtgggctcgt cgctcgcccg
    10621 ggaggccgac ggcgtgatct acatgcacgc gggcccggag atcggcgtgg ccgccacgaa
    10681 gacgttcgcg acccagatgg tgtcgcagta catcctcgcg atgtacctcg cgcaggtccg
    10741 aggctcgatg ttcccggagg agatcgcaga ggtgctgacg cggatggggg agctcccggc
    10801 gcaggtcgaa cgagcgatcg ggctggacgc gcaggtccag gagctcgctc ggcggttcca
    10861 ggactcgcgc gactggctgt tcatcgggcg gcacaccgga tatcccgcgg ctctcgaagg
    10921 agccctgaag ctgaaggaga tcagttacgt gcacgcggag ggctatccgg ccggcgagct
    10981 gaagcacggc ccgatcgcgc tggtcgagga cggcgtgcct gtggtggcgg tcgcgaccac
    11041 gtgccacgtc tatccgaaga tgctctcgaa catccaggag gtacgcgcgc gcggcgccga
    11101 ggtgatcgcc gtggcgacgg agggggacga gcagatccgc ggtgtggccc agcacgtgct
    11161 cgaggttccg gagacgccgg agctgctgtc gccggtcgtg gtgacggtcc cgctgcagct
    11221 cctcgcgtac cacgtcgccc ggttgcgtgg ttccgacgtc gaccagccga ggaacctcgc
    11281 gaagagcgtc acggtcgagt agcgtcgggg catggcccgg gtgatggctc gtccccgcgc
    11341 tctccggctc atgctcctcg cggtgctcgt cgcgggcctc ctcgtcccgg gcgcgccagc
    11401 acgagcgtgt tcctgtgccg agatggacct cggttccctg cttccagacg ccgacggggc
    11461 gttcgtgggc acgttcgtcg accggagcga gatcagcgac cagctcgcct cgatcacgtt
    11521 cgacgtggag cgtgtcgtga agggcgagtt cggcccgagg gcgatcgtgc ggacgaacgc
    11581 gtacggcgcc tcctgcggcc tcgagttcct cgacgggcca cggatcggtc tgctgctcga
    11641 ccgagcgtca gacggcgtgt gggagtcgag cctgtgtcag caggtacaac ccggcgatct
    11701 cctcgccatc ggcggagaac gaccgcccga ccctgacgtc gctcccgtga gtgccggatg
    11761 gacgctcggg tcccaggggt tggtcggaac gatcgtcgca gccgctctgg tgctcgtcgt
    11821 gctggtgtgg gtcatccgtc ggcgttcctc gcggccgggc agcggtctga cgtagccttc
    11881 ctgctgtgga catcgccggg ctgggggtcg acctatgcga gatcgcgcgg atggagcggg
    11941 cgatctctcg acacccgacg ttccgtgacc gcgtcttcac cccggaggag atcgcgtact
    12001 gtgactcgaa ggctcgcccc gcggagtcgt acgcggggcg cttctccgcc cgcgaagcca
    12061 cgatcaaggc gctcggcggg taccgaggcc ggcgctggca ggacatcagt gtgacccgac
    12121 acccgtcggg agcgcccgcg atccggctcg acggcaacgc caagcgacgg gcggacgccc
    12181 tcggggtcac ccaggtgctg atcacgttca cgcacgagcg gacgaacgcg gtggcgttcg
    12241 cgatcgcggt ccgatgaagc cggtcctgac gccggaggag gccgccgccc tcgaccgcga
    12301 cacgcaggcg cgcggcatcg gggccgaagt cctgatggag cgggcgggcc gcgcggttgc
    12361 gcgggccgcc cgcgagctcg ccggcggctc ctacggccgt cgagcggtcg tcgtctgcgg
    12421 gaagggcaac aacgggggcg acgggctcgt cgcggcgcgt cacctcgccc gcgagggtgt
    12481 gcggacgatc gtggtgctgc tcgaggatcc cgacgagctc cgcgatcccg cggcgtcgaa
    12541 cgcccggcgc ctcgccgagg tcccgggcat ccgcgtgcgg acgttcgacg aacacgccct
    12601 ccggcgtgag ctcgtccgag cggacatcgt ggtggatgcg atcttcggga cggggttccg
    12661 cggcatgccg gaggacgact gggcgagcgc gatcgagagc ctgaacgagg ccgacgcgcc
    12721 ggtggtggcg gtcgacatcc cgtccggcgt cgatggtgcg acgggggtcg tcgagggtga
    12781 ggcggtccgt gcggacctga ccgtcgcgtt cggcgcgccc aagctgggca ctgtcctgat
    12841 gcccggcgcg gagcatgcgg ggatcctgcg ggtcgtcgac atcggtttcc ccgacgacct
    12901 tctccgagct cgcgcctggc tcacggagcc ggacgacgtg gccgggtggc tgccccaacg
    12961 tgacatcgac acgcacaagc gcgcttcggg ggtactggtc gtcgtggcgg ggtcgcgtgc
    13021 gatgaccggg gcggcccgcc tgatcgcgat gtccgcgggc agggtgggca ctgggctggt
    13081 tgtcgtggcc gctcccgaag actgcctgtc gcagatccaa gcggggctga ccgagccgac
    13141 gttcttgcca ctccccgaga cggcggacgg caccgtggct gcggaggcgg ccgcaccagt
    13201 gctggaacgg ctggaagctg cggacgcgct ggcgatcggg ccgggcctca cgaccgacca
    13261 ggcgacggtg gccttcgtcc gcgacgtcgt gcgtcgttca ccggtccccc tcgtcctcga
    13321 cgccgacggt ctgaacgcgt tcacggccga cgcgactgcg ctcgaggacc gtgcgtccga
    13381 ggccgtcctc acaccgcacg tcggcgagtt cggccggttg accggcgtga aagctcgcga
    13441 cctcgacgcc gatcgccccg ctcacgtccg ggcgctggcg gatcagacgg gtgccgtggc
    13501 gctcctcaaa gggagtcgca gcgtgatcgc gaaccccgag gggcggttgt tcgtgaacgt
    13561 gaccgggtcg cccacgctcg ccaccgccgg cacgggtgac gtcctgaccg ggatgatcgg
    13621 cgggctcctc gcgcgggggg tgccgccgac cgagtcggct gccgcggccg cctacctcca
    13681 cggcctggcc gggctcttcg cgggcaggga cctcggtgaa ggagcgatcg cgggagatgt
    13741 catggaccgt gtgcccgagg cggtcgcgca cgtggagggc aagtgagcgc cgagtgagcg
    13801 ccgagtgagc gccgagtgag cgccaagtga gcgaaagcta tcggcccacc tgggtcgagg
    13861 tggatctcga agcgatccga cacaacgccc gcgtcctcaa gcccgagacc gccgagttga
    13921 tggcggtggt gaaggcgaac ggctatgggc acggcgacgt ggaggtggcc cgcgccgcga
    13981 tcgacgccgg cgccacctgg tgcggggtgg ccctcgtcga ggaaggtctt cggctccgtt
    14041 ccggggggat cgaggcgccg atcctcgtgc tctcggagct cccggccggt tccgaggccg
    14101 tcgccctcgc gcatcggctc acgccgacgc tgtattcgga cggagcgctc gagcgtctcg
    14161 cgtccgcgac acgaggatcg gtgcccgtgc atgtgaaggt cgataccggg atgcaccgcg
    14221 tgggggtctg gccaccggag gacgctccgg cgttcgcacg acgtgtgcag gaggccggcc
    14281 tcgaggtcga gggtctcttc acgcacttcg cccggtcgga ggaggacgag gccaccacca
    14341 aggagcagct cgagcgattc ctcgataccg ccgacgccgc tcggaccgcc ggcgttcgtc
    14401 cgcgggtcct ccactcagcg aattccgggg cgacgatcct gcatccggag tcccacctgg
    14461 acctcgtgcg gcccggcatc gccctgtacg ggatcgaacc ggcgccgggg atcggcgcac
    14521 atctgggtct gcgcccggcg ctcacctggc ggagccgggt gagcgccgtg aagcggctcc
    14581 ctgccggcga ggccgtctcg tacgggcacc ggtaccggct cgaacacgat gcctggctcg
    14641 ccaccgtgcc ggtcggctat gcggacggct accctcggca gctgacgaac ctcgggtcgg
    14701 tggtgatcgg gggtcggcgc cacgggatcg ccggtacggt cacgatggac cagctgctcg
    14761 tggactgcgc ggacgaggag atccaggtcg gcgaggaggt cgtgctgatc gggaaccagg
    14821 gcgacgagac gatcggggcc gaggagctcg gccgactcat cgggacgatc gggtacgaga
    14881 tcgtgagccg gatcggcgac cgcgtcccga ggcggtacgg atgaggaagg tcgtgtcgaa
    14941 cgcgcccaga gtggccgtgg gtgcggctgc cgccgcggga gtggtcgccg ccggcctcgc
    15001 gggtcgagcg ctctggcgac atcggcgtcc ggccggcggc gaccgcgcgt ggggcccgcc
    15061 cccggaggat ctgggaccga tcgaagcgtt cgacggcgcg gagctcgtcg tgcgcgcggc
    15121 cggtccgccg gatggctcga tcctgctgtt ctcccatggg ttcagcctcg acatgacgac
    15181 ctggcgcgaa cagtggtccg acctcggcga cgagtaccgc tgcatcgccc tcgaccatcg
    15241 cgcgcacggt cggagcgagg tgccggggac cggcgacgtg agcctccggt cgatggggag
    15301 ggacctcgcg accgtcctgg acgcggtcgc acctgaccgg cgcgtcgtgc tgatcggtca
    15361 ctcgatggga gcgatggcga tcctcgccct cgccgagcag cgtccggacc tcttcggtcc
    15421 acggatcggg ggcatcgtgc tcctcggggc atccgcttcg gatctgttgc gcggcgcgat
    15481 gggcggcgtg accgacctcc tacgaccccg cctcggatcg ctcgccgcgg ccgctcgccg
    15541 ggtggatcgc ctccgtcgcg cgatcctcgc cggtccggcg gaggtgccgg ccatcgtcgc
    15601 gcgcgtgacg cagttcgctc ccgaggcgcc ggacgatgtc gtcgaccacg tgctgcatct
    15661 cgccgagcag acctcttccc tcgtctggac ggacggcctg gcggagctga tgcggatgga
    15721 cttccgccac gccctcgcgc ggatcacggt gcctgcgctc gtcgcagttg gagaccagga
    15781 ccgcgtgacg ccgcccgccg tcgccgtgga gctcgccgcg gcgctcccgg atgggaggct
    15841 gttcctcgtg gagggagcag gtcacatcgc gatgttggag cggcccgaac ggctcaaccc
    15901 ggagctccgc gcgttcgcgc gggaggccct ctcgttcgga tcggatcgtg gccggaagcg
    15961 ccgggggacg aggaagaacg gggcggcgtg agccggacgc tggcggacat cggtgccgag
    16021 gcggcggggt gcacccggtg ccggctcgcc gaggggcgga cccaggtcgt gttcggcgtg
    16081 gggaacccgg atgcggacgt gctgttcatc ggtgaggctc ccggtttcca cgaagacaag
    16141 cagggtgagc cgttcgtcgg ggccgccggg cagctgctca cgaaggtcct cggggacgtc
    16201 ctcggactcg accgtggcga cgtctacatc tgcaacatcc tgaagtgccg gccgcccggg
    16261 aaccgcgatc cgcaggagga cgagatcgag tcctgtacgc cgtggctcgt ggaacagatc
    16321 tcgtcgatcc aaccgcgcgt gatcgtgacg ctcgggaact tcgcgacgaa gttcgtcctg
    16381 cagacccagc aagggatcac gcggatgcgc gggaaggcct atccatggca cggcaggacg
    16441 gtgatcccga cgttccatcc ggccgcgatc ctgcacggcg gtggggagaa gtcgcgccag
    16501 ttcggtgagc tccgcgagga cttcgagctc gtacgcgaca cgctcgctgc gatggacccg
    16561 gggcccgggt cggcgccggc acccgagtcg tctccggcac ccgttcaggt ccccgcgccc
    16621 gcgacggtcc gtgtgccgga cgccggtgtc gcgccggtgc ccgaggagca gctcgagctg
    16681 ttctgatcat gcgcctcgag ctcgcgaccg ctgccgccga cggaacgcgg gacgtcggtg
    16741 cggccctcgc gaccctgctc ctgcctcgcg ataccgtgat cctcaccggg gatctcggag
    16801 ccggcaagac gacgctggtc caagggatcg ggagaggact cggggtggag gatcacgtcg
    16861 cctcgccgac gttcacgctg gtgaaggagt acgcggggag cctcgacgtc gcccatgtcg
    16921 acgtgtaccg gctcgagcgc gtgcaagacg tcgtcgacct cgggctcgac gagctgggcg
    16981 gccccgatcg ggtgctcctg gtcgagtggg gggacgcggt ccaggagctc ctgcccgagg
    17041 accggctccg agtggagctg accacggggt cggacgacga cgcccgtcgg atcgtgctca
    17101 caccacacgg acgatcgtgg gcgctgcggt gggaacggct cgagcagctc ctcgaaccgt
    17161 tccgccgaga cggaccgtcc ggtggcccgg tcgcggaggc tgccccgtga tcgtcgtggg
    17221 gatcgagacc tcgacgccgc agacgtcggt ggcgatcggc tcggagcgtg agctgctcgg
    17281 ggcggtctcg gtggccggca aggctcgcca agaggcggtc acgcctgcgc tggagcgact
    17341 ctgcggatgg accggcgtcg agctcagcca ggtgggcggg atcgccgtcg ggatcgggcc
    17401 ggggctgttc acggggctgc gcgtcggcat cgagaccgcg aagacgctcg cccaggtcct
    17461 cagcgtcccg atcgtcggga tcacgagcct ggacgcgttg gcgttctcgg tccgtcacac
    17521 gcacaagctg atcgtcgcgg tgatcgacgg gcgccgacgg gaggtgttct tcgcgatcta
    17581 ccgggcgctc ccgggcgggg tcgtccgagg gagcgagtac accgtggcga cgcccgatca
    17641 cctctgcgcc gagctggaaa ccgtgccggg ggagatgctt gcggtgggcg acggtgccat
    17701 cctgtaccgg aaccagctgg agg
//
LOCUS       DASUYD010000246         6933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_855736, whole genome shotgun sequence.
ACCESSION   DASUYD010000246 DASUYD010000000
VERSION     DASUYD010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 6933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6933
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855736"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF195_13005"
     CDS             complement(<1..488)
                     /locus_tag="VF195_13005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEX6581772.1"
                     /translation="MPVSSEPAARLVFLGGVGEVGRNMACLELDERILIVDVGLSFPS
                     GDMPGIDLVLPDFDFLRTKADRIQAVVLTHGHEDHIGALPYLLRDVERRLEVYGTPFT
                     LALLQGKLEEHEVEHLVDLRTVSPGEQASTGPFTMRFLRVTHSIPDGMAVVVDTPFGS
                     IL"
     gene            complement(472..1356)
                     /gene="dapA"
                     /locus_tag="VF195_13010"
     CDS             complement(472..1356)
                     /gene="dapA"
                     /locus_tag="VF195_13010"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX6581773.1"
                     /translation="MAGRFGSVVTAMVTPFRSDHGVDLDGAQELASWLTDNGSDSIFV
                     VGSTGESPTLSHNEKADLFRAVGEAVRGKGKLICGTGTYSTAETLELTQAAEDAGADG
                     IVVVTPYYNKPPQRGLVAHFEHIAEATELPIIVYNIPSRTGTRIEHETMLRIAEKPNV
                     VAVKDSTGDFKAISNLISEAPEGFEVYSGDDWATFGYVCLGAVGVVSVASHLVGLQIR
                     QMVDLIETGDVPAARKLHEDLSPLFNALFVTSNPIPVKAALDMLGRPAGPPRLPLVPA
                     TADERERIQRALEDAGLL"
     gene            1440..1982
                     /locus_tag="VF195_13015"
     CDS             1440..1982
                     /locus_tag="VF195_13015"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEX6581774.1"
                     /translation="MPRIYTKTGDDGTTGLLYGGRVSKDDAATEAYGTTDEAVAVLGL
                     ARASAEPVMAEELLALQRELFIVGADLATNPSERAKLQPGVSLVTSDMTERLERRIDE
                     LVAERALPEAFIVPGANEGSAALDLARSVIRRAERQVVALEHEGRDVNPEVRRFLNRL
                     SDLVFVLARRRAGEDEPTSR"
     gene            complement(2017..3231)
                     /locus_tag="VF195_13020"
     CDS             complement(2017..3231)
                     /locus_tag="VF195_13020"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX6581775.1"
                     /translation="MDLEIRSITYEERPEWVRAAETAFSSLAKDDEVEASLPVIEPDR
                     SFAAIDGGRIVGTSAAITFRMMVPGGARIPTAGVTMVGVHPTHRRRGINTEMMRVVLD
                     QAAERGEPLAALFASEGAIYGRFGYGLAGLFGEFQAESARMAFVRGYEPGGRVELVSK
                     EEALPVIDEVYDAAMRPGGVERNAALRDHNFATVGEDKDRPWMYAVHRDDAGTADAYA
                     VYWMKHDWPRSVPTGTITVKECVAWTPSGNADIWRFLFDIDLVATVEAWNRPADEPLL
                     HLLREPRRLRFSMNDGLWVRLLDVVKALEARRYGTDGRLVFDVTDPFRPDTDGRYELI
                     AEGGEGRCTRTDGAADLAGTINVLGATYLGGSSFRQQWWAGQVEERTPGSLERADVMF
                     SSTPAPWCVVDF"
     gene            complement(3275..4045)
                     /gene="dapB"
                     /locus_tag="VF195_13025"
     CDS             complement(3275..4045)
                     /gene="dapB"
                     /locus_tag="VF195_13025"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007513439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEX6581776.1"
                     /translation="MIRVGVIGATGKVGAEVCRAVDADPDLELVAGISRSKAGEPAST
                     ALGLDGSDVVLSEDLSSFVDARVEVAVDFTSASFAPEHVAWSIEHGVHIVVGTTGFQI
                     DEGWRQAPVGVFVAPNFAIGAALMQRFAREAARYLPAVEILELHHEGKADAPSGTAAA
                     TADAIADARPESWAAGPSSGSIPGVRGGERAGIRIHSIRLPGLVAHQEVIFGGQGQTL
                     TIRHDSMDRTSFVPGVLTAIRWVPTHPGLTVGLDAILE"
     gene            complement(4042..5316)
                     /locus_tag="VF195_13030"
     CDS             complement(4042..5316)
                     /locus_tag="VF195_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015624861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX6581777.1"
                     /translation="MGIERTEFSSGLRVVTERMPGVRSVSLGFWVLAGSRDEPPVISG
                     SSHFLEHLLFKGTKTRSAREIAEDFDAVGGDVNAFTSKEYTCYYARILDGDLEMAVDH
                     LSDMFQHSALRSADLDAERQVILEEINMHEDSPEEVVHDLFTEALWPNHPLGRPVLGT
                     AETIRAATRVKVHRFYRRHYVPGGLVVAAAGNLRHEELLDMLRGRMDTGRIVRSGDPQ
                     GWNLRAGETAPESSAKPLVKRRKTEQAHICLGTNGLARSDPDRFPFLIVNGALGTGMS
                     SRLFQEIREKRGLVYSVYSYHSQYTEAGLFACYAGTTPARAKEVIGLLRRELDDVAEG
                     GLTAEEFDRAKGHVKGSMVLSLEDPGSRMSRIGKSEIAHGEILTVNQSLKRVEDVSLE
                     DARRVAKRVLSQPMTLTVLGPFGQRDLAGVMA"
     gene            complement(5341..>6933)
                     /locus_tag="VF195_13035"
     CDS             complement(5341..>6933)
                     /locus_tag="VF195_13035"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX6581778.1"
                     /translation="LQEEFLAEVGVKPSDFDPHPLYQQDLWDALESFGKDKVLSALVS
                     DRKEREANMSVVKDEAKAHLAQTLGEDVFAARASEFSLAWKSLEKKVMRARVISDGVR
                     LDGRGPKDIRPLAAHVGLLPRAHGSSLFERGDTQVLNVTSLGMLRMNQMIDTLDPEDS
                     KRYIHHYNFPPYSTGETGRVGSPRRREIGHGALAERALIPVIPSEEEFPYTLRLVSDV
                     LSSNGSTSMASVCASTLSLMDAGVPVKAPVGGIAMGMIAEGDTFVTLTDILGAEDALG
                     DMDFKVAGTREFVTAIQLDMKVSGMPADVLAGALQQAREARLTILDVMEAAIPAPRDA
                     VNPIAPRIITITIPVDKIGEVIGPKGKRINEIIAQTGADIDIQDDGTVFIGSHEGEGA
                     DEAARMIDEIANPRPVLQGETYDGTVVKTTTFGAFVNLVPGRDGLVHISKLGKGKRLS
                     SVEEAVREGDKLTVLVEDIDAQGKISLKPTGPEWEVPEGEGSSGGERREPREPREPRN
                     GADRGPRRTGRRFRDADKGSSA"
BASE COUNT         1060 a         2459 c         2321 g         1093 t
ORIGIN      
        1 tggaggatcg agccgaacgg cgtgtcgacc acgaccgcca tcccatcggg gatcgagtgg
       61 gtgacgcgga ggaaccgcat cgtgaacggg cccgtcgacg cctgctcccc cggcgagacg
      121 gtcctgagat cgacgaggtg ctccacctcg tgttcctcga gcttgccctg cagcagcgcg
      181 agcgtgaacg gcgtgccgta gacctcgaga cggcgctcga cgtcgcggag caggtatggg
      241 agcgctccga tgtgatcctc gtgcccgtgc gtgaggacga ccgcctggat ccgatcggcc
      301 ttcgttcgca ggaagtcgaa gtccggcagg accaggtcga tcccgggcat gtcgcccgac
      361 gggaacgaca gacccacgtc gacgatcagg atgcgttcgt ccagctcgag gcacgccatg
      421 ttgcgaccga cctcgccgac cccgccgagg aagacgagtc gcgccgcagg ctcagaggag
      481 accggcatcc tccagtgctc gttggatccg ctcgcgctcg tccgcggtgg cgggcaccaa
      541 cggcagccgt ggcggacccg ccggccggcc cagcatgtcc aacgcggcct tcacggggat
      601 cgggttggac gtgacgaaga gcgcattgaa caggggcgac aggtcttcgt gcagcttgcg
      661 ggcggccggc acgtcgcccg tctcgatcag gtcgaccatc tgcctgatct ggaggccgac
      721 gagatgcgat gccacgctca cgacgccgac cgcgcccaga cacacgtagc cgaacgtcgc
      781 ccagtcgtcg cccgaataca cctcgaaccc ctcgggcgct tccgagatca ggttcgagat
      841 cgccttgaag tcacccgtgg agtccttgac ggcgacgacg ttcggcttct cggcgatccg
      901 gagcatcgtc tcgtgctcga tgcgggttcc ggtgcggctg gggatgttgt agacgatgat
      961 cggcagctcg gtggcctcgg cgatgtgctc gaagtgcgcg accagtcccc gctgcggcgg
     1021 cttgttgtag tacggcgtga cgacgacgat gccgtccgcg cccgcgtcct cggccgcctg
     1081 cgtcagctcg agtgtttcgg ccgtcgagta ggtccccgtc ccgcagatca acttcccctt
     1141 gccgcggacc gcctccccca ccgcacggaa caggtccgcc ttctcgttgt gggacagcgt
     1201 gggcgactct ccggtcgagc ccacgacgaa gatcgagtcg gagccgttgt ccgtgagcca
     1261 ggacgcgagc tcctgcgcgc cgtcgaggtc gacgccgtgg tcggaacgga acggcgtgac
     1321 catcgcggtg acgacggatc cgaaccttcc ggccacccga atcctcctcg gtcttggcgg
     1381 gcctcgcgat ccggtcggat cggcggggcg cccgcagcga cgccctatgc tagctcccca
     1441 tgccgcgcat ctacacgaag accggcgacg acggcaccac ggggctcctc tacggaggac
     1501 gggtctcgaa ggacgacgcg gcgaccgagg cgtacgggac gaccgacgag gccgtggccg
     1561 tgctgggact cgcacgcgcc tcggcggagc ccgtgatggc cgaagagctc ctcgcgctcc
     1621 agcgagagct cttcatcgtc ggcgcggatc tggccacgaa cccgagcgag cgcgcgaagc
     1681 tccagccggg cgtatcgctc gtgacgagcg acatgaccga acggctcgag cggcggatcg
     1741 atgagctcgt cgccgagcga gcactccccg aggcgttcat cgttcccggt gcgaacgagg
     1801 ggagcgcggc gctggacctc gcccgcagcg tgatcaggcg ggcggagcgg caggtcgtgg
     1861 ccctggaaca cgagggccgc gacgtgaacc ccgaggtccg ccgcttcctc aaccggctga
     1921 gcgaccttgt gttcgtcctc gcccgccgcc gagccggcga ggacgaaccg acgagccgct
     1981 agaacgttct gcacgggatc acccgggacc tggtgatcag aagtcgacga cgcaccaggg
     2041 tgccggcgtg gaggagaaca tcacatcggc ccgctcgagc gaaccgggcg tgcgttcctc
     2101 gacctgtccc gcccaccact gctgacggaa gctcgacccg cccagatagg tcgctcccag
     2161 gacgttgatc gtgccagcga ggtcggctgc cccgtcggtg cgggtgcagc gaccctcgcc
     2221 gccttcggcg atcagctcgt accggccgtc ggtatcgggg cggaacggat ccgtgacgtc
     2281 gaacaccagc cgcccgtcgg tcccgtagcg tcgggcctcg agcgccttga caacgtccag
     2341 gagccggacc cagagcccgt cgttcatgga gaaccgcagg cgtcgcggct cacggaggag
     2401 atggagcagc ggctcgtcgg ccgggcggtt ccacgcctcg accgtcgcca ccaggtcgat
     2461 gtcgaacagg aagcgccaga tgtccgcgtt gccggacggg gtccaggcca cgcactcctt
     2521 gaccgtgatc gttcccgtcg gcaccgagcg gggccagtcg tgcttcatcc agtagaccgc
     2581 gtaggcatcc gccgtcccgg cgtcgtcgcg gtgcaccgcg tacatccacg ggcggtcctt
     2641 gtcctctccg accgtcgcga agttgtgatc gcgaagcgcc gcgttccgct ccacgccgcc
     2701 gggccgcatc gcggcgtcgt agacctcgtc gatgaccggc aacgcctcct ccttcgaaac
     2761 gagctcgacc cggccgcctg gctcgtatcc tcggacgaac gccatccgag cggactctgc
     2821 ctggaattcg ccgaacaacc cggcgaggcc gtacccgaag cgtccgtaga tcgctccctc
     2881 ggaggcgaag agcgccgcga gcggctcgcc ccgttccgca gcttgatcca ggacgacccg
     2941 catcatctcg gtgttgatcc ctcgtcggcg gtgggtggga tggacaccca ccatcgtgac
     3001 gccggccgtc gggatcctcg cgccaccggg gaccatcatc cggaacgtga tggcggccga
     3061 cgttccgacg atcctgccgc cgtcgatcgc cgcgaacgag cgatcgggct cgatgaccgg
     3121 gagcgaggcc tccacctcgt cgtccttcgc cagggagctg aaggcggtct cggcggcccg
     3181 aacccactcg ggtcgctctt cgtacgtgat cgaacggatc tcgaggtcca tgcggccgag
     3241 cctaccgtcg gcggcgggtc gtccggccgg ccggctactc gaggatcgcg tcgagaccga
     3301 cggtcaggcc ggggtgggtc ggcacccatc ggatcgccgt caacactccg ggcacgaacg
     3361 acgtccggtc catcgagtca tggcggatcg tgagcgtctg tccctggcca ccgaagatga
     3421 cctcctggtg cgcgacgagg ccggggagcc ggatcgagtg gatccggatc cccgcccgct
     3481 cgccaccgcg gacgccgggg atcgatcccg aggacggacc cgctgcccag gactccggac
     3541 gggcgtccgc gatcgcgtcc gccgtcgcgg ccgccgtccc cgacggtgcg tcggccttgc
     3601 cttcgtgatg gagctcgagg atctcgacgg ccggcaggta ccgggcggcc tcgcgcgcga
     3661 accgctgcat cagggcggcg ccgatcgcga agttcggagc gacgaagacg ccgaccggcg
     3721 cctgccgcca cccttcgtcg atctggaagc cggtcgtacc gaccacgatg tgcacgccgt
     3781 gctcgatcga ccacgcgacg tgctccggcg cgaagctcgc gctcgtgaaa tcgacggcga
     3841 cctcgacccg ggcgtcgacg aacgagctga ggtcctccga gaggaccacg tcggagccgt
     3901 cgaggccgag cgccgtggag gcgggctcgc ctgccttgga ccgtgagatg ccggcgacga
     3961 gctcgaggtc cgggtcggcg tcaaccgcgc gacacacctc ggcgccgacc ttgcccgtgg
     4021 cgccgatcac gccgacgcgg atcacgccat gacccccgcg aggtctcgct gtccgaacgg
     4081 ccccaggacc gtcagcgtca tcggctgcga cagcacccgc ttggccaccc gtcgtgcgtc
     4141 ttcgagcgac acgtcctcga cgcgtttcag cgactggttc accgtgagga tctcgccgtg
     4201 cgcgatctcg gacttcccga tcctcgacat ccggctcccg gggtcctcga gcgagagcac
     4261 catcgacccc ttgacgtgtc ccttcgcgcg atcgaactcc tcggccgtca gaccgccctc
     4321 cgcgacgtcg tccagctcgc ggcggagcag gccgatcacc tcctttgcgc gggccggcgt
     4381 ggtccccgcg tagcaggcga acagccccgc ctccgtgtac tgcgagtgat agctgtacac
     4441 cgagtacacg aggccgcgct tctcgcggat ctcctggaag aggcgcgagg acatcccggt
     4501 tcccaacgcg ccgttcacga tcaggaaggg gaaccggtcc gggtcgctcc gcgcgagccc
     4561 gttcgtgccg aggcagatgt gggcctgctc ggtcttgcgc cgcttcacca acggcttcgc
     4621 ggacgactcg ggcgcggtct cgccggcgcg gaggttccat ccctgtggat cccccgaccg
     4681 cacgatccgt cccgtgtcca tacgaccgcg gagcatgtcg agcagctcct cgtgccggag
     4741 gttccccgca gccgccacga cgagtccgcc cggcacgtaa tgcctgcggt agaaccgatg
     4801 gaccttgact cgcgtcgctg cgcggatcgt ctcggccgtt ccgaggacgg gccggcccag
     4861 cgggtggttc ggccagaggg cctccgtgaa caggtcgtgg acgacctcct ccggagagtc
     4921 ctcgtgcatg ttgatctcct cgaggatcac ctggcgctcc gcgtcgagat ccgccgaccg
     4981 cagcgccgag tgctggaaca tgtcggagag gtggtcgacc gccatctcca ggtcgccgtc
     5041 gaggatgcgc gcgtagtagc aggtgtactc cttcgacgtg aacgcgttca cgtcgccgcc
     5101 gacggcgtcg aagtcctccg cgatctcgcg ggcggacctc gtcttggttc ccttgaagag
     5161 caggtgctcg aggaagtggc tcgagccgct gatcaccggc ggctcatcgc gggatccggc
     5221 gagcacccag aagccgagcg acacggagcg gacgcctggc atccgctccg tgacgactcg
     5281 caggcccgag gagaactcgg ttcgctcgat ccccactgtt cgcctccgga tggttccgcc
     5341 ttaagccgag gatcccttgt cggcgtcgcg gaaccgacgt ccggtccgac gcggcccgcg
     5401 gtctgccccg ttgcggggct cgcgcggttc gcgtggctcg cgacgctcgc cgcccgagga
     5461 accctcgccc tccggcacct cccattcggg accggtcggc ttcaagctga tcttgccctg
     5521 ggcgtcgatg tcctccacga gcacggtgag cttgtcgccc tcccggaccg cttcctccac
     5581 cgagctgagg cgcttgccct tgcccagctt cgagatgtgc acgagaccgt ccctgccggg
     5641 cacgaggttc acgaacgccc cgaacgtcgt cgtcttcacg acggtgccgt cgtaggtctc
     5701 accctggagg acgggacgcg ggttcgcgat ctcgtcgatc atccgggccg cttcgtcggc
     5761 gccctcacct tcgtgggaac cgatgaacac cgtgccgtcg tcctggatgt cgatgtcggc
     5821 accggtctgg gcgatgatct cgttgatccg cttccccttg gggccgatca cctcgccgat
     5881 cttgtcgacc gggatcgtga tcgtgatgat ccgtggcgcg atcgggttca ccgcgtcgcg
     5941 cggagcaggg atcgccgcct ccatcacgtc gaggatcgtg aggcgcgcct cgcgggcctg
     6001 ctgcagcgca cccgcgagca cgtccgcagg catcccgctg accttcatgt cgagctggat
     6061 cgcggtgacg aactcccgcg tgccggcgac cttgaagtcc atgtcgccca gcgcgtcctc
     6121 cgctccgagg atgtccgtca gggtcacgaa cgtgtcgccc tcggcgatca tccccatcgc
     6181 gatcccgccg accggcgcct tgacgggcac gccggcatcc atcagcgaga gcgtcgacgc
     6241 gcacacgctg gccatcgacg tcgagccgtt ggaggacaga acgtcggaaa ccagtcgcag
     6301 cgtgtagggg aactcctcct cggaggggat cacggggatc agcgcccgct cggcgagcgc
     6361 cccatgaccg atctcccggc gacgaggcga gcccacgcgg cccgtctccc cggtggagta
     6421 cggcgggaag ttgtagtggt ggatgtaccg cttggagtcc tcgggatcga gggtgtcgat
     6481 catctggttc atccggagca tcccgagcga cgtgacgttg agcacctgcg tgtcaccgcg
     6541 ctcgaacaac gacgacccgt gggcgcgggg caggagcccc acgtgggccg cgagcggccg
     6601 gatgtccttc gggccgcgac cgtccagccg gacgccgtcc gagatcaccc gcgcccgcat
     6661 gaccttcttc tcgagcgact tccacgccag cgagaactcg ctcgcgcgag cagcgaacac
     6721 atcctctccc agcgtctggg cgaggtgggc cttcgcctcg tccttgacga cggacatgtt
     6781 cgcttcgcgc tccttccggt cggagaccag agcagagagc accttgtcct tcccgaacga
     6841 ctcgagcgcg tcccagaggt cctgctggta gagcgggtgc gggtcgaagt ccgaaggctt
     6901 cacaccgacc tcggccagga actcctcctg gag
//
LOCUS       DASUYD010000247         2028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_421297, whole genome shotgun sequence.
ACCESSION   DASUYD010000247 DASUYD010000000
VERSION     DASUYD010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421297"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1340
                     /locus_tag="VF195_13040"
     CDS             189..1340
                     /locus_tag="VF195_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009604667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HEX6581779.1"
                     /translation="MAFDMSQVQRGARNRRTPYHEATQKHDPKGFTVYNHMYFPIRFD
                     SFEEEFETLLTGVTLWDVAVERCLEIGGPDGFRFAQLLTPRDLSTCAVGQGKYVLICD
                     SDGGIVNDPVLTRMDENTFWFALASSDALLFARGLKNAYPELDVTIREADVAPLQVQG
                     PKSRDLMAKLLGPEILDLRYYFWTESKIAGAPVVITRTGWTSEVGYEVYLTDTTKGVE
                     VYEAIMEAGQEFGIRPTGPSDIRRIEGAIFNWGADMTYDNNPLEMGLERLVDWGLDDA
                     SSISMAALRSIKERGVRQTVNGLELDGDPFPELNNLKWPVVDAGERIGAVTSAIYSPR
                     LERNIGFAWLPIERSTLGERVTVETEWGPRHGVVVEMPFVDPGKQIPVS"
     gene            1518..>2028
                     /locus_tag="VF195_13045"
     CDS             1518..>2028
                     /locus_tag="VF195_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018038189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX6581780.1"
                     /translation="MLEQSATIYFGPWYRRSPFFDATRRAGCTAYDVYNHMLLPAYFD
                     DPVTEYWALLNDVTVWDVGVERTVEISGKDADRFIDTLTCRDLTTCAVKQGKYMIVTA
                     PDGGIVNDPVLLHVGENRWWMQLADSDAGLYALGVAAVSNLDVEVSYPHAYPMQVQGA
                     KAAKTLEKLV"
BASE COUNT          342 a          651 c          719 g          316 t
ORIGIN      
        1 ccgacgcgtc cgtcgtgctc acgttcgtag acggggagcg cgtgttcgat gccgaacggc
       61 cgggctgacg tcgggtcggc gctgaccagc ggtcatgcaa gctcgttgcc aggcgtcggt
      121 gtcggacgga cgatggccgc ggtcccgacc cgggccggac gacccaccca gcgcgaggag
      181 gttccgtgat ggcgttcgac atgagccagg tgcagcgtgg cgcgcgcaat cggcgcacgc
      241 cctaccacga agcgacgcag aagcacgacc cgaaaggctt cacggtctac aaccacatgt
      301 acttcccgat ccgcttcgac tcgttcgagg aggagttcga gacgctcctc acgggggtga
      361 ccctgtggga cgtcgcggtc gagcggtgcc tggagatcgg ggggcccgac ggtttccgct
      421 tcgcccagct gctgacgccg cgcgacctct cgacgtgcgc ggtcgggcag ggcaagtacg
      481 tgttgatctg cgacagcgac ggggggatcg tcaacgaccc ggtcctcacg cggatggacg
      541 agaacacctt ctggttcgcg ctcgcgtcct ccgatgccct gctgttcgcg cgggggctga
      601 agaacgcgta cccggagctg gacgttacga tccgggaggc cgacgtggct ccgctgcagg
      661 tccagggacc gaagtccagg gacctgatgg ccaagctcct cggtccggag atcctggacc
      721 tccgctacta cttctggacc gagtcgaaga tcgccggcgc gccggtggtg atcacgagga
      781 ccggatggac gtcggaggtc gggtacgagg tctacctcac ggacacgacc aagggcgtcg
      841 aggtctacga ggcgatcatg gaagccgggc aggagttcgg catccgtccc accggcccga
      901 gcgacatccg ccggatcgag ggcgcgatct tcaactgggg cgccgacatg acgtacgaca
      961 acaacccgct cgagatgggc ctcgagcgcc tggtcgactg gggcctggac gacgcctcca
     1021 gcatctcgat ggcggcgctc cgctcgatca aggagcgagg cgtgcggcag acggtcaacg
     1081 gtctggagct ggacggcgac ccgttccccg agctcaacaa cctcaagtgg ccggtggtgg
     1141 acgcagggga gcggatcggc gcggtgacct ccgcgatcta ctcgccgcgt ctcgaacgca
     1201 acatcgggtt cgcgtggctg ccgatcgagc gctcgacgct cggcgagcgc gtcacggtcg
     1261 agacggagtg ggggccgcgc cacggcgtcg tcgtggagat gccgttcgtc gaccccggca
     1321 agcagatccc ggtctcctag tcccggggcc gttcgggccg ttcgaacctg aagttcggtt
     1381 cagtcgcatc gccccgtcgc aggacgcggc gcggcttgcg ggcgacctgc gattgaacga
     1441 tgattcaatg cgtcgacggc cgaccggccg gccgatcgaa gggggtcgtg cccggaatgg
     1501 tgaacgaacg gcaagagatg ctcgagcaga gcgcgacgat ctacttcgga ccgtggtacc
     1561 ggcgttcgcc gttcttcgac gcgacccgtc gggccggctg cacggcgtac gacgtctaca
     1621 accacatgct cctccccgcc tacttcgacg acccggtcac cgagtactgg gccctcctga
     1681 acgacgtcac ggtctgggac gtgggcgtgg agcgtaccgt cgagatctcg ggcaaggacg
     1741 cggatcggtt catcgacacg ctgacgtgcc gggacctgac cacctgcgcg gtcaagcagg
     1801 ggaagtacat gatcgtcacc gcgccggacg gcgggatcgt caacgacccg gtgctcctcc
     1861 acgtggggga gaaccggtgg tggatgcagc tcgccgactc cgatgcggga ctgtacgcgc
     1921 tcggcgtggc ggcggtgtcg aacctggacg tcgaggtgtc gtatccccac gcctacccga
     1981 tgcaggtgca gggcgcgaag gcagcgaaga cgctcgagaa gctcgtcg
//
LOCUS       DASUYD010000248         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1082280, whole genome shotgun sequence.
ACCESSION   DASUYD010000248 DASUYD010000000
VERSION     DASUYD010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082280"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2166)
                     /gene="valS"
                     /locus_tag="VF195_13050"
     CDS             complement(<1..2166)
                     /gene="valS"
                     /locus_tag="VF195_13050"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012893207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX6581781.1"
                     /translation="MNVPEKPTIDGLEAKWGERWETDGIYRFDRSKTRDQVFAIDTPP
                     PTASGTLHVGSVCSYTHTDLIARFQRMRGKVLFYPMGWDDNGLPTERRVQNFFGVRCD
                     PSVPYESAFDLDALDRSGDEPVAVSRPNFIDLCERLTAEDEKAFEGLFRVLGLSVDWS
                     QTYRTIDARSRRVSQRVFLRLLRRGIAYQAEAPTMWDVDFRTAVAQAEIQDREVDGRY
                     QRIRFNLADGSGSLEVETSRPELIPACVALVVNPSDGRYRSLVGSTAVTPLFSAEVPI
                     VAHELADPEKGTGVAMICTFGDVTDVTWWRELKLPTRVVVQRDGTIAPPRWGEPGWRS
                     RDVEAARRAQEEIAGLTSKRARTRIAELLAGSGDLLGEPTPVRHVVKFYENGQRPLEV
                     VASRQWFVKTIEFRDRLLERGRELRWHPPHMGARYASWVEGLNGDWGISRQRFFGVPI
                     PLWYPLDHDGVPDHDHPLVPSEDRLPVDPSTDVPDGYSEDRRGKPDGFVGDPDVMDTW
                     ATSSQTPEIAGGWEEDEDLFGRVFPMDLRPQAHDIIRTWLFYTVLKAELVHGKLPWTD
                     AAISGFILDPDRKKMSKSKGNVVTPIALLEQHGADAVRYWAASARLGLDAAFKEDQIK
                     IGRRLAIKILNASRFTLSLEGTEAEIADPLDRSMLDQLSGVVEQATVALEGYEHARAL
                     ELVERFFWGFTDDYLELVKGRAYGGHGPEAAGSAIASLRF"
BASE COUNT          354 a          774 c          744 g          366 t
ORIGIN      
        1 gaaccgcagc gacgcgatcg ccgatcccgc agcctcgggg ccgtggccgc cgtacgcgcg
       61 tcccttcacc aactcgaggt agtcgtccgt gaacccccag aagaagcgct cgacgagctc
      121 gagcgcccgg gcgtgctcgt agccttcgag cgcgaccgtc gcctgctcga cgacgccgga
      181 gagctggtcg agcatcgagc ggtcgagcgg gtccgcgatc tcggcctcgg taccctcgag
      241 cgacagggtg aagcgtgagg cgttcaggat cttgatcgcg agccgtcgtc cgatcttgat
      301 ctgatcctct ttgaaggcgg catccagacc gagtcgtgcg ctcgcggccc agtaacggac
      361 ggcgtcagcc ccgtgctgct cgaggagggc gataggcgtg accacgttcc ctttggactt
      421 cgacatcttc ttgcgatcgg ggtcgaggat gaatccgctg atcgcggcgt cggtccacgg
      481 aagcttcccg tggaccagct cggccttcag gacggtgtag aagagccagg tcctgatgat
      541 gtcgtgggct tgcggacgaa ggtccatcgg gaagacgcga ccgaacaggt cctcgtcctc
      601 ctcccacccg ccggcgatct ccggcgtctg ggaggaggtg gcccacgtgt ccatgacatc
      661 ggggtctccg acgaacccat ccggcttgcc gcgtcgatcc tccgagtacc cgtccgggac
      721 gtcggtgctc gggtcgaccg ggagccgatc ctcgctcggg accagcgggt ggtcgtgatc
      781 gggcacgcca tcgtggtcga gcggatacca cagcgggatc ggaacgccga agaaccgctg
      841 ccggctgatg ccccaatccc cgttcaggcc ctcgacccaa gaggcgtacc gtgcccccat
      901 gtgcggcggg tgccaccgca gttcgcgccc gcgttcgagg agtcggtcgc ggaactcgat
      961 cgtcttcacg aaccactgcc ggctggcgac gacctcgagc ggtcgctgcc cgttctcgta
     1021 gaacttcacc acgtgccgca ccggcgtcgg ctcccccagc aggtcccccg acccggcgag
     1081 gagctcggcg atccgcgtgc gcgcccgctt cgaggtgagg ccggcgatct cctcctgcgc
     1141 ccggcgagcc gcctcgacgt cccgcgacct ccatccgggc tccccccatc gcggcggtgc
     1201 gatcgtgcca tctcgctgga caacgactcg ggtggggagc ttcagctctc gccaccacgt
     1261 gacgtcggtg acgtcgccga acgtgcagat catcgcgaca ccggtcccct tctcgggatc
     1321 cgccagctcg tgcgcgacga tcgggacctc ggcggagaac agcggcgtca cggccgtcga
     1381 gccgaccagc gaccggtacc ggccgtcgga cgggttcaca acgagtgcga cgcaggccgg
     1441 gatgagctcg ggtctcgagg tctcgacctc caacgaaccg gagccgtctg ccaggttgaa
     1501 tcggatgcgt tggtaccggc cgtccacctc tcggtcctgg atctcggcct gcgcgaccgc
     1561 cgtccggaag tcgacgtccc acatcgtcgg cgcttcggcc tgatacgcga ttccacgacg
     1621 aaggagccga aggaacacgc gctgcgagac ccgtcgtgag cgcgcgtcga tggttcggta
     1681 cgtctggctc cagtccaccg acaggccgag cacgcggaac agcccctcga acgccttctc
     1741 atcctcggcg gtcagtcgtt cgcacagatc gatgaagttc ggacgcgaga cagccaccgg
     1801 ctcgtccccg gaacggtcga gcgcgtcgag gtcgaaggcg gactcgtacg gcaccgaggg
     1861 gtcgcaccgg acgccgaaga agttctggac ccggcgctcc gtgggcaggc cgttgtcgtc
     1921 ccatcccatc gggtagaaga ggaccttgcc ccgcatccgc tggaagcggg cgatgaggtc
     1981 ggtatgcgta tacgagcaga ccgacccgac gtgcaacgtg ccgctcgcgg tcggcggcgg
     2041 ggtatcgatc gcaaagacct ggtctcgcgt cttcgaacga tcgaaccggt agatgccgtc
     2101 ggtctcccac cgctcgcccc acttcgcttc gaggccgtcg atcgtcggct tctcgggcac
     2161 gttcatgcgc gggagtctaa gcgatggccg atcgtcgacc ggatgcatac cgtcgggctc
     2221 gccgtccgga ggcggtcg
//
LOCUS       DASUYD010000249        18686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1083313, whole genome shotgun sequence.
ACCESSION   DASUYD010000249 DASUYD010000000
VERSION     DASUYD010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 18686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18686
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083313"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..305)
                     /locus_tag="VF195_13055"
     CDS             complement(9..305)
                     /locus_tag="VF195_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581782.1"
                     /translation="MKDLKKVLVSLTISAMVLAGILVATQAPSWGRHDLPSFSHEQLE
                     ADRVMTQQMGTDVGPSMAVQMPGDGMLKRSSSEAYVEALEQHTRTYNKMAGLTP"
     gene            complement(311..553)
                     /locus_tag="VF195_13060"
     CDS             complement(311..553)
                     /locus_tag="VF195_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011780846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2933 domain-containing protein"
                     /protein_id="HEX6581783.1"
                     /translation="MEANDVKRTKLGSRVLVASVLVVIAVTLFRVPLATVFTLGVLLI
                     CPLLMMGMHGAHGGGHGDHAASGDDVRVSPGRHEGR"
     gene            complement(720..965)
                     /locus_tag="VF195_13065"
     CDS             complement(720..965)
                     /locus_tag="VF195_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HEX6581784.1"
                     /translation="MMWGNGWGDWVAGVLMMVLFWGGLVALVFLVLRSWDTNRRRDGG
                     NPDRPHARTILEERFARGEISEEEFEQRRRVLEHATH"
     gene            complement(973..1341)
                     /locus_tag="VF195_13070"
     CDS             complement(973..1341)
                     /locus_tag="VF195_13070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581785.1"
                     /translation="MKKTWIVTGIVVVSLVAATAGIAFAAGRGSGNTRSPAAGQMAAA
                     HDAMHDSVEMQAMHDQMPAALQAKCEALHEQMDQMMASMMSGSGMMGGSGMMDDSGMM
                     GDDVGITGGSMADHHSSTEG"
     gene            complement(1338..2768)
                     /locus_tag="VF195_13075"
     CDS             complement(1338..2768)
                     /locus_tag="VF195_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581786.1"
                     /translation="MRRSFALRLAAAFGGVGIAAAALTAILVNGAFGDRFAGYLDAQQ
                     RTRERQLVAILADSYASSGRWDTADLRSLTPLAFLDGGELRLLDASGTTVWAPSPVEL
                     LGQMAEMHRQMMGSGVLGPERRLRIVVDGEEVGTAVVQLPATGLLPQDLSFRASVNRL
                     LLYGGVAAGLVALPVGFLLARRATAPARELARAARGFASGDRTRRVPYETRDEFGEMA
                     RAFNAMGDAVEEEDRLRRGFAAEVAHEVRTPLAIIRSQIEAIQDGVIEPNPPAVASLH
                     EETLRLTRLMGDLETLASADAAGFSLSPQVVSLGPLLDETVRGFAARFVGQGVTLHTE
                     LDDVTADVDPNRMRQVAANLLSNALKFTPPGGSVTLSLRSEHGQAVITVVDTGPGISA
                     EDLPHVFDRFFRGQGVRASGSGIGLTVVRELIEAHGGSVAVSSELGAGATFAVRLPAA
                     SSGPREEFTSPSQGSSTVVSEGGDER"
     gene            complement(2761..3456)
                     /locus_tag="VF195_13080"
     CDS             complement(2761..3456)
                     /locus_tag="VF195_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007498313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581787.1"
                     /translation="MGSSTILVVDDEQKIRDLVRSYLELEGYSVLVADTGARALESAS
                     RSNPDLVVLDLGLPDLPGEEVAQTLRAASNVPIIMLTAKAGEDDRVAGLRLGADDYLA
                     KPFSPRELVARVEAVLRRANGSRPHRLSFDDGRLEIDRDSWEVVLDGSAVELTRSEFD
                     LLYALASRAGRVFSRYELINRVQGYDYEGYERTIDQHVKNLRRKLGEDTKHPRYVLTV
                     TGVGYRFGAKADA"
     gene            complement(3495..3764)
                     /locus_tag="VF195_13085"
     CDS             complement(3495..3764)
                     /locus_tag="VF195_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020153694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6581788.1"
                     /translation="MGDNHDGILKRLRRIEGQVSGIRKMQEGGRYCIDVLDQISAARA
                     GLEATALLILQDHVNGCVREAIEEGKGEEKAAELLEAVGRFARSV"
     gene            4013..4294
                     /locus_tag="VF195_13090"
     CDS             4013..4294
                     /locus_tag="VF195_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013118872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HEX6581789.1"
                     /translation="MRNVTSSQHRPVEIVVVRSPACHLCEDAIEALDEMRARSSLDVR
                     LVEMESEEGRDIVARHRPALFPAVLVEDRLFSSGRLPRRKLERILARGG"
     gene            4295..5275
                     /locus_tag="VF195_13095"
     CDS             4295..5275
                     /locus_tag="VF195_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011757215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis CcdA family protein"
                     /protein_id="HEX6581790.1"
                     /translation="MGELLSGGSILAAFFGGAVALFSPCCIVFLLPSYMAAGVKNRRW
                     RLLPLTLVFAVGLGLVLLPITLGVGLLAQALSDFHTPLYVAGGLLLLAMAALALSGRS
                     WSLPSFIRSPAPDRSDTAGVFSLGVFSGIASSCCAPVLAGVMTLSALSSSIGGSAALG
                     LAYVFGMVFPLFLLAALWDRLRLGERRPFQSRPVRLRIARLTIITNTVNVAIAAVFAA
                     MGGFVLYLAAAGNPMGSGGPQLAVGRWLTAFFSWVLAFLDPVPDLVLGLALLALAAGV
                     VTFALKGGREVPPSAESGGSCHEHHEDETEKDAHRPARQAAGDEEAVVTR"
     gene            5250..5831
                     /locus_tag="VF195_13100"
     CDS             5250..5831
                     /locus_tag="VF195_13100"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEX6581791.1"
                     /translation="MRKRSSRGSRWPSAWSLGIGVAVIGAVTAMLLASRSPGSSEGAG
                     DEAPAFTLASTAGDPVSLADYRGQDVLLYFSEGAGCDSCFYQMADLEDNAAKLSASGL
                     SVVPIVVNPADQVLAEMGRFGLTTPFLIDADRSVSDAYGVVGTGMHADLPGHSFVLVD
                     GSGRIAWRGDYPSMFVSADQLLSDIGAAGRAPA"
     gene            5908..6924
                     /locus_tag="VF195_13105"
     CDS             5908..6924
                     /locus_tag="VF195_13105"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX6581792.1"
                     /translation="MELTRRDVLKGAGVVATGAGFASLLTEPAFAHGDEGGEGEEHPT
                     SRVRRWAMVIDLRACDGCVGLGIPPQCTQNCIWARFVPEDQQWIETYELPEGSEHRPP
                     GTPRSFLPAPCMQCQNAPCVNVCPVGATFHTPEGVVLIDQERCIGCRYCMAACPYDRR
                     FFNWGEPLQPPDVAEAPYDVRTQLPAMRGTVMKCDFCTDRLAAGGLPSCVDGCPRNAI
                     YMGDLEEDVATNGARMVRLSSLLAEAGVHRHKEELGTEPRVYYIAGHGEAAGRDASEI
                     GHGADATSVVMGGEQGDEAGHEEGASETPSTLPADLSALPFLKDRLEWPWRRIVAALE
                     EMDR"
     gene            6924..8276
                     /gene="nrfD"
                     /locus_tag="VF195_13110"
     CDS             6924..8276
                     /gene="nrfD"
                     /locus_tag="VF195_13110"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX6581793.1"
                     /translation="MDVIGHDDLEETTLAPLRRTTPSWFVLVAVLAALVAFGLYAYTT
                     QLRRGLVVTGLAPTLNKVMWGLYITNFVFFIGISHAGTLISAILRVSHAGWRTPITRM
                     AEFITVVALSVGALFPLIDLGRPDRMWNVFQYGRWPSAVMWDFFAISTYFVGSVLYLY
                     LPMIPDLALCRDLLASGASRARRWLYRTLAMGWRGEPEQRRHLAAAIGLMMIVIIPVA
                     VSVHTVVSWIFAMTLRTGWDTAVFGIYFVAGAIFSGIATLILTMAILRKTLHLEPWIT
                     AQHFTNLGYLMAALALLMIYFNLSEFVTAGYKLSGSERVYLGEILTGSFAPMYWAYVV
                     LGLFVPMLIVVVPPLRRVGLIVTAALLVDVAMWVERYIIVVASLRVPQMPYPDPARYL
                     PSWVEVAISLGSLALFALIVALFVKVFPVVSVWEIREHSASAVAETAPAGLRKEVVEL
                     "
     gene            8273..9412
                     /locus_tag="VF195_13115"
     CDS             8273..9412
                     /locus_tag="VF195_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581794.1"
                     /translation="MSKSAFRLSVLVVGLVAAAILATPTAALAHGEPPGSQDEPSVMP
                     EPEPQGQLERLRVVLDLPEEASVGGATTIRAVVTHREDGDPVEGLMVTFEAPATWGEA
                     LAGSMVLGTAVTDHDGTAVIATEIRAAGDLEVTARVESAEGFREASTTASLEVVRQRQ
                     VVEDEVGLRIPWLNLWVLAGVIGLVWLLYLTAAGHLRAIARQPDQPPDASPTQAMSRR
                     TLLGRSFPLAVQAGIAAFGAGLIGIVVRSPRTHGNLLSPPGTEAYRRTPVAFVGRSDS
                     MREMPTPLDRPVSFRDEVLPVFMRFGGPHVVQPEHSPPPGRLQLDSWAHLMEAGVVVP
                     GKPEESELVEHLLSPGMQMPPSRPPLPDDTIQLIVTWVAQGAQDN"
     gene            complement(9529..10214)
                     /locus_tag="VF195_13120"
                     /pseudo
     CDS             complement(9529..10214)
                     /locus_tag="VF195_13120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722097.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
     gene            complement(10294..11325)
                     /locus_tag="VF195_13125"
     CDS             complement(10294..11325)
                     /locus_tag="VF195_13125"
                     /EC_number="1.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX6581795.1"
                     /translation="MSPIKVGVNGYGVIGKRVADAVTVQPDMELIGVADTATDYRVKL
                     AAARSYPLFGSTPQAVGDFEQAGLEPSGGLDELLQQVEVIVDATPKNVGAANAPAYEA
                     AGVKAVFQGGEKHELTGLSFVAQANYAEALDRDSVRVVSCNTTGLVRVLSALRERGLV
                     ARTRAVLIRRGTDPWESHQGGLINTLVPEAIIPSHQGPDAKTVIPELDVVTIAAAGPF
                     NLSHVHFLVVEAPREIDRDEVLDALGDASRIAFVRVSDGVAAPNSVIEIVRDLGRPRA
                     DLWEVALWEDTLAVSGREVFLNYQVHNEAIVVPENIDAIRAITGEEPEAAKSIAATDQ
                     SLGIRQRLP"
     gene            11498..12259
                     /locus_tag="VF195_13130"
     CDS             11498..12259
                     /locus_tag="VF195_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017600090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX6581796.1"
                     /translation="MSVAIDAARFDAVVFDLDGVLTDTARVHQAAWKLLFDEVLGAIE
                     GTIDRRPFDREDYRSYVDGRARVDGVEAVLTSRGARISRGRPDDPPGTTTAWALANRK
                     NEHFLAALAAEGVRAFPDAASLVRRVRAAKLGTAVVTASQHRADVLAAARIDELFDVH
                     VDGIDAASLGLAGKPDPAMFLEASRRLGVRPARAVVLEDALAGVAAGRAGRFGLVIGV
                     DRTGDRDALEAAGAHVVVTNLGVIDVGGAKGGPLP"
     gene            12256..14676
                     /locus_tag="VF195_13135"
     CDS             12256..14676
                     /locus_tag="VF195_13135"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX6581797.1"
                     /translation="MRGWELVYEGFDPAHQGHREVMCALGNGYMATRGALAESIADGV
                     HYPGTYVAGIYNRLASEVGGRTIEQETIVNLPNWLPLAFRSEGGSWTAPASVRLLEHR
                     LVLDLLEGVLHRWSRVEDLEGRITAVSERRLVSMSSAHLAALEQVITPENWSGVLEIR
                     SGLDGRVENANVAEDRLLSNVHLAPLGEGSEDGGTTWLEVETTQSHVRAAVAARVRLR
                     SADEEAITTGPRRVPREPGRVIEEVPVEVTAGRRVVVEKIASIWTSRDRAQSDPLSAA
                     QSDVAEEQGFDGLLADHRGAWEHLWERCRFDLGETEEDVTLLVRLHAFHMLQTISPHV
                     ADLDVGVPARGLHGEGYRGHVFWDELFVLPLFNLRIPELARALLLYRARRLPAALRAG
                     RAMGMPGARFPWQSGSDGRELTPAQLWNPRSQRWVADNSRLQHHVSLAIAWNLWQYYQ
                     ATGDERFLAEYGIEMLVQIARFWAGLAIREPRDGRFDIRGVMGPDEFHDGYPDRPGAG
                     IDNNAYTNVLASWTLQRAVEVRHLLCEQPDRHLWDRLGVTDSEVESWGHISQRLRVPF
                     HDGMVSQFQGWERLAELDWEGYRSRYGNIGRLDLILESEGDSTNRYKLAKQADALMLF
                     YLFSADELGALFDHLGYEFDPQTIPATVEYYIARTTHGSTLSHLVHAWVVARSNRSGS
                     WEVFRESLVADLDDTQGGTTAEGIHLGAMGGGLDALQRCYTGLEMRGDALRLAPRLPD
                     GLRSLGFDMTYRGHQLTLSVDPERMKITAHPCSAAPVDLMVGGRAITLAADQTVEVPV
                     REDGVEGA"
     gene            complement(14715..16145)
                     /locus_tag="VF195_13140"
     CDS             complement(14715..16145)
                     /locus_tag="VF195_13140"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX6581798.1"
                     /translation="MSEERGCDRSEHYRSLSLSRRRFLKASGLALAGAGLYACTGGGR
                     KLPVVAPNVTGSVVATETRWPIKRAIYLMMENRSFDNLFGRFPGANGSLVGVKNGREV
                     RLLPSPHWLPGDLPHDRAAHLNDANGGAYDGFGNGRFGDPWAYTTFDSDKVPAYWQLA
                     KDYVLCDNFFASVGGPSYPNHFFFVAGQSGGAIDNPENIEVRIDGGNRRFKSWGCDAV
                     GDDVFVFVRDEEGNLTKHDTCFSLPTVPEQLEKASVSWAYYSASRHQTGYFWNALNGI
                     AGVFHTDLWRPGDTIRPVDDLLEDIAADRLPSVTWITPRFELSDHPPQSVCFGQNWVA
                     DVVNAVMRSAAWRHTVIFLTWDEWGGLYDHVPPPEIDPLGLGFRVPMLVVSPFAKEGY
                     IDDAEGEFSTPLRFIEDNWGLPYLTPRIERTHNFEHVFDFRRDPRMNARPVPRFDDCY
                     GTPWEYPGDGYPGWPAGTQPDPSQFV"
     gene            complement(16185..16940)
                     /locus_tag="VF195_13145"
                     /pseudo
     CDS             complement(16185..16940)
                     /locus_tag="VF195_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543487.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(17916..18311)
                     /locus_tag="VF195_13150"
     CDS             complement(17916..18311)
                     /locus_tag="VF195_13150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581799.1"
                     /translation="MPKDEEPLAVYYSWEDDARVAPVLVVLRSSLVGIELTLLERTDN
                     LFSIAYSAVSIHDVARVSSTGRAWIGGELGVKLEEIDIDLNRDLPPFSATIHLPVARN
                     DYRGDRDDAREAARAVADAVMSIGFGPIP"
     gene            18452..18523
                     /locus_tag="VF195_13155"
     tRNA            18452..18523
                     /locus_tag="VF195_13155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18488..18490,aa:Arg,seq:cct)
BASE COUNT         2945 a         6454 c         6134 g         3153 t
ORIGIN      
        1 gggctcgcct acggcgtgag cccggccatc ttgttgtacg tccgggtgtg ctgctccagg
       61 gcttcgacgt atgcctcgct cgaagatcgt ttcagcatgc cgtctccagg catctggacc
      121 gccatgctgg gacccacgtc ggtccccatc tgctgagtca tcacgcggtc agcctcgagc
      181 tgctcgtggg agaaggacgg cagatcgtgc ctcccccacg agggtgcctg cgtcgccacg
      241 aggatgcccg cgaggaccat cgccgagatc gtgagcgata ccagtacctt cttcaggtcc
      301 ttcacgtcga tcaccttccc tcatggcggc ccggagatac gcggacgtcg tcccctgagg
      361 ccgcgtgatc gccatggcca ccaccgtggg ccccgtgcat ccccatcatg aggagggggc
      421 agatcagcag cacacccagg gtgaacaccg ttgcgagcgg aacgcgaaag agcgtcaccg
      481 ctatcacgac cagcaccgat gcgaccagga ctctcgatcc gagcttcgtt cgcttaacgt
      541 cgttggcttc catgtcggtc tccttcatct cgtcacgggg accgtagagc cgccgtgtga
      601 agggcccatg aagaaccgaa cgattcggga tgaggacgag agacggcgct cgggcaggtg
      661 tgcgaacgcg aaggaggctg ccggcgagtg gccggcagcc cttccgcctt ccgatcgtct
      721 caatgagtcg cgtgttccag cactctgcgc cgctgctcga actcctcctc ggagatctct
      781 cctctggcga agcgctcctc caagatcgtc cgtgcgtggg gcctgtctgg gttcccgccg
      841 tcccgccgcc tgttggtgtc ccagctccgg agcacaagga acacaagcgc cacgagccct
      901 ccccagaaga gcaccatcat gaggacgccc gctacccagt cgccccagcc gtttccccac
      961 atcatcgtcg tctcacccct ccgtcgatga gtggtggtcg gccatcgacc caccggtgat
     1021 cccgacgtcg tcgcccatca tgccggagtc gtccatcatg ccggagccgc ccatcatgcc
     1081 ggagccgctc atcatcgagg ccatcatctg gtccatctgc tcgtgcaagg cctcgcactt
     1141 cgcctgaagc gcggctggca tctgatcgtg catggcctgc atctccaccg aatcgtgcat
     1201 cgcgtcgtgc gctgccgcca tctggcctgc ggccggggat cgggtgttgc cgctcccacg
     1261 gcctgcggcg aaagcgatcc ccgccgtcgc tgcgacgagg ctcacgacca cgatccctgt
     1321 gacgatccat gtcttcttca tcgctcatct cctccttcac tcacgaccgt agatgagccc
     1381 tgtgagggtg atgtgaactc ctcgcgcggt ccggatgaag ccgcggggag gcgcaccgcg
     1441 aacgtggcgc ccgccccgag ctcgctggag accgcaaccg accccccatg tgcctcgatc
     1501 agctcacgga ccaccgtgag cccgatcccc gacccgctcg cccgcacgcc ctgccctcgg
     1561 aagaaccggt cgaacacgtg cggaagatcc tccgcggaga tccctggacc cgtgtcgacg
     1621 acggtgatca ccgcttgccc atgctcggat cggaggctca acgtcaccga cccccccgga
     1681 ggcgtgaact tcaaagcgtt cgacaggagg ttcgccgcca cctggcgcat ccggttgggg
     1741 tccacgtccg ccgtcacgtc gtcgagctcg gtatggagcg tcacgccttg acccacgaac
     1801 cgcgcggcga accctcgaac ggtctcgtcg agcagcggcc cgagtgacac cacctgcggg
     1861 gacagcgaga acccggctgc gtcggccgac gccagggtct cgagatcgcc catcagtcgc
     1921 gtgaggcgga gcgtctcttc gtggagggaa gcgaccgccg gagggttcgg ttcgatcacg
     1981 ccgtcctgga tcgcctcgat ctgggatcgg atgatcgcga gcggggttcg gacctcgtga
     2041 gcgacctcgg ctgcgaagcc tcgccggagc cgatcctcct cctccacggc gtcgcccatc
     2101 gcgttgaagg cacgggccat ctccccgaac tcgtcgcgcg tctcgtaggg cactcggcgc
     2161 gtccgatccc cggaggcgaa gcctcgcgcg gcgcgcgcaa gctcgcgggc cggtgccgtg
     2221 gcacgccggg caagcaggaa ccccacgggc aaggccacga ggccggctgc aacaccgccg
     2281 tacaggagaa gacggttcac cgacgcccgg aacgagaggt cttgaggcag cagaccggtg
     2341 gccggcagct gcaccaccgc ggtgcccacc tcttctccgt cgacgacgat ccgaagtcga
     2401 cgctcgggtc caaggacgcc actgcccatc atctgccgat gcatctccgc catctggccc
     2461 aggagctcga cggggctggg tgcccagacg gtcgttccag atgcatccag caggcggagc
     2521 tccccaccgt ccaagaacgc cagcggcgtg agcgaccgga gatcggccgt gtcccacctc
     2581 cccgagctcg cataggagtc ggccaggatg gcgacgagct gccgttcgcg ggtccgctgc
     2641 tgcgcatcga gatagccggc gaatcggtcg ccgaacgcgc cgttcaccag gatcgcggtg
     2701 agcgcggcgg cggcgatccc taccccgccg aacgcggccg ccagtcggag ggcgaacgac
     2761 ctacgcatcg gccttcgccc cgaaccggta gccgacgccg gtcaccgtca ggacgtaccg
     2821 gggatgcttc gtgtcctccc ccagcttccg acgcaggttc ttcacgtgtt ggtcgatcgt
     2881 tcgctcgtag ccctcgtagt cgtagccctg gacccggttg atcagctcgt atcgagagaa
     2941 gacacgaccg gcgcgtgacg ccagcgcgta cagcaggtcg aactcgctcc gggtgagctc
     3001 cacggccgag ccgtcgagca cgacctccca tgagtcgcga tcgatctcga ggcgcccgtc
     3061 atcgaaggac aatcggtgcg ggcgcgaccc gttcgctcga cggaggacgg cctccacgcg
     3121 ggcgacgagc tctctcgggc tgaacggttt cgcgaggtag tcgtcggcgc ctaggcggag
     3181 acccgcgacg cggtcgtcct cgccggcctt ggctgtgagc atgatgatcg gaacgttgga
     3241 tgcggcacgg agcgtttggg cgacctcttc gcccgggaga tcgggcagcc ccaggtcgag
     3301 cacgacgagg tcggggttcg atcgggaggc cgattcgagc gcccgcgccc ccgtgtccgc
     3361 gacgaggacc gagtacccct ccagctcgag gtacgagcgc acgaggtcgc ggatcttctg
     3421 ctcgtcgtcc accacgagga tcgtcgagga gcccacccta cgcctccgtc gccgtcgcct
     3481 gattctcgtt cacgtcacac gctcctcgcg aaccgaccga cggcctccag cagctcggca
     3541 gccttctcct cgccctttcc ttcttcgatc gcttcgcgga cgcacccgtt cacgtggtcc
     3601 tggaggatca ggagggccgt cgcctcgagg cctgctcggg cggcggagat ctggtcgagg
     3661 acgtcgatgc agtaccggcc gccctcctgc atcttccgga tgcccgagac ctgcccttcg
     3721 atccggcgga gccgcttcaa gatgccgtcg tggttgtctc ccatggccac ctctccgggt
     3781 cagtccctct cagggatcga cggattgccg taccccagtt gggcatatct tcggcactga
     3841 gcagcggccg ttcatgtgcc tcaggtcccg ccaccgaggg gaccaacgtc cgtgctcggc
     3901 cagatccaag gcacacagag cccacgcggg ctcttagcag ggcgaacgcg gcttcatgcg
     3961 atacgcggac gcccttcaca cggtcttcac aacgtctgcc gatgctgcac gcatgcggaa
     4021 cgtgacatcc tctcagcatc gaccggtcga gatcgtggtc gtgcgttcgc ccgcctgtca
     4081 tctgtgcgag gacgccatcg aggcgctcga cgagatgcgc gccagatcct cccttgatgt
     4141 tcgtctggtc gagatggagt cggaggaggg ccgcgacatc gtggcccggc atcggccggc
     4201 cctcttcccc gccgtgctgg tcgaagaccg gctgttcagc tcgggccgac tccccaggag
     4261 gaaactcgag cggatcctgg cgaggggcgg ctaggtgggc gagctgctct ccggaggctc
     4321 catcctcgcg gcgttcttcg gcggcgccgt cgcgctgttc agcccttgct gcatcgtgtt
     4381 cctgcttcct tcctacatgg cggcgggcgt gaagaaccgg cgctggcggt tgctaccgct
     4441 cacgctcgtg ttcgcggtgg ggctgggact ggtcctgctc ccgatcaccc tgggggtggg
     4501 cctgctggca caagcgctgt ccgacttcca cactccgctc tacgtcgcag gcgggctgct
     4561 gctgctcgcg atggccgccc tcgccctgtc gggacgatcg tggagcctgc cgtcgttcat
     4621 ccggtcgcca gcccccgacc gcagcgacac cgccggcgtg ttctccctcg gggttttctc
     4681 gggcatcgcc tcgtcgtgct gcgcaccggt gctcgccggc gtcatgacgc tgtcggcgct
     4741 gtcgtcctcg atcggcggct ccgccgcctt ggggctcgcc tacgtcttcg ggatggtgtt
     4801 cccgttgttc ctcctcgcag cgctctggga tcggcttcgg ctcggggaac gccggccgtt
     4861 ccagagcagg ccggtccggc ttcggatcgc caggctcacg atcatcacga acaccgtgaa
     4921 cgtcgcgatc gcagccgtgt tcgccgcgat gggcggcttc gtgttgtacc tggccgccgc
     4981 ggggaacccg atgggctcgg ggggtccgca gctcgccgtg ggccggtggc tgaccgcctt
     5041 cttctcctgg gtcctcgcgt tcctcgaccc ggtgcccgat ctcgtcctcg gcctggcgct
     5101 tctcgctctc gccgcgggcg tcgtcacgtt cgcactcaag ggtggccgcg aggttccacc
     5161 ctccgccgaa tcgggaggtt cctgtcatga gcaccacgaa gacgagaccg agaaagacgc
     5221 gcaccgcccg gcccgccagg cggcgggcga tgaggaagcg gtcgtcacgc ggtagccgct
     5281 ggccgtcggc ctggtcgctc gggatcggtg tggcggtgat cggggccgtc acggcgatgc
     5341 tcctcgccag ccgctcaccc ggctcgtccg agggcgccgg ggatgaggcc ccggcgttca
     5401 cgctggcttc gaccgccggc gacccagtgt cactcgccga ctaccgcggc caagacgtcc
     5461 tcctctactt cagcgagggc gcgggctgcg actcgtgctt ctaccagatg gcagatctcg
     5521 aggacaacgc tgcgaaactc tcggcgagcg gactgtcggt ggtcccgatc gtcgtgaacc
     5581 cggcggacca ggtgctcgcc gagatggggc ggttcgggct cacgaccccc ttcctgatcg
     5641 acgcggaccg gagcgtatcc gacgcgtacg gtgtcgtcgg caccggcatg catgcggatc
     5701 tcccaggcca ctcgttcgtc ctggttgacg gctccggccg gatcgcatgg cgtggtgact
     5761 atccgtcgat gttcgtcagc gccgatcagc tgctctcgga catcggcgcg gccggccggg
     5821 cgccggcgtg aggccgtccc caacggcctg cccggacagg gaccaacggc ccttgtcctc
     5881 tccacgcggg agaggaccct ccttcccatg gagctcacgc gccgagacgt gctgaagggt
     5941 gccggggtcg tagccaccgg cgcggggttc gcctctttgc tgaccgagcc ggccttcgcg
     6001 cacggcgacg aaggagggga gggcgaggag catccgacct cgcgcgtgcg acggtgggcg
     6061 atggtgatcg acctccgcgc gtgcgacggt tgcgtcggcc tcgggatccc cccgcagtgc
     6121 acacagaact gcatctgggc tcgcttcgtc cccgaggacc agcagtggat cgagacgtac
     6181 gagctccccg aaggcagcga acaccgcccg ccgggcacac ctcgctcgtt cctccccgcg
     6241 ccctgcatgc agtgccagaa cgctccatgc gtgaacgtct gccccgtcgg cgctaccttc
     6301 cacacgcccg agggggtcgt gctgatcgac caggaacgct gcatcgggtg ccggtactgc
     6361 atggccgcat gcccctacga ccggaggttc ttcaactggg gcgagccgct ccagccgccg
     6421 gacgtcgccg aggcgccata cgacgtgcgg acccagctcc cggcgatgcg tggcacggtc
     6481 atgaagtgcg acttctgcac cgaccggctg gccgcgggtg ggcttccgtc gtgcgtcgat
     6541 ggatgtcccc gcaacgcgat ctacatgggc gacctcgagg aggacgtcgc cacgaacggg
     6601 gctcgcatgg tccggctgtc gagtctgctc gcggaagccg gagtccaccg ccacaaggag
     6661 gagctgggga cggagccgcg cgtgtactac atcgccgggc acggcgaggc tgcgggccgg
     6721 gacgcaagcg agatcggtca cggagctgat gcgacctccg tggtcatggg gggcgaacag
     6781 ggagacgaag cgggacacga ggaaggagcg tctgagacgc cgagcacgct ccctgcagac
     6841 ctgtctgcgt tgccgttcct caaggaccgg ctcgaatggc cgtggcgacg gatcgtcgcg
     6901 gccctcgagg agatggatcg ctgatggacg tgatcgggca cgacgatctc gaggagacca
     6961 ccctcgcgcc cctccggagg acgacgccat cctggttcgt gctggtcgca gtgctcgcgg
     7021 ccctcgtcgc gttcgggctc tacgcctaca cgacacagct gcggcgaggg ctggttgtga
     7081 caggcctggc gccaacgctc aacaaggtca tgtggggctt gtacatcacg aacttcgtgt
     7141 tcttcatcgg gatcagccac gcgggcacct tgatctctgc gatcctccgt gtgtcccatg
     7201 cgggatggag gacgccgatc actcggatgg ctgagttcat caccgtcgtg gcgctctcgg
     7261 tgggggccct gttccccctc atcgatctcg gacgcccgga tcggatgtgg aacgtgttcc
     7321 agtacgggcg ctggccgtcc gcggtcatgt gggacttctt cgcgatcagc acctacttcg
     7381 tcggaagtgt cctgtacctg tacctgccta tgatcccgga cctcgccctg tgccgcgatc
     7441 tgttggcctc gggagcgtca agagcgcgac gctggctcta ccgaacgctc gcgatgggat
     7501 ggcgcggcga gccggaacag cgacgccact tggctgcggc gatcggtctg atgatgatcg
     7561 tcatcatccc cgtcgcggtg tccgtgcaca cggtcgtgtc ttggatcttc gccatgacgc
     7621 tccgaacggg ctgggacacg gcggtcttcg ggatctactt cgtcgccggc gcgatcttct
     7681 caggaatcgc aacgttgatc ctgacgatgg ccatcctgcg caagaccctt cacctggagc
     7741 cttggatcac cgcacaacac ttcacgaacc tcggctacct catggccgct ctcgccctcc
     7801 tgatgatcta cttcaacctc tccgagttcg tgaccgccgg ctacaagctg agcggcagcg
     7861 agcgcgtcta ccttggcgag atcctcaccg gtagcttcgc tcccatgtac tgggcgtacg
     7921 tggtcttggg cctgttcgtg ccgatgctga tcgtcgtggt gccaccactg cggagggtcg
     7981 gcctgatcgt caccgccgct ctcctggtcg acgtcgccat gtgggtcgag cgctacatca
     8041 tcgtcgtggc gagccttcgg gtcccgcaga tgccgtatcc ggatcccgcg cggtacctgc
     8101 ccagctgggt cgaggtggcg atctcgctcg gctcgctcgc cttgttcgct ctgatcgtgg
     8161 ccctcttcgt gaaggtcttc ccggtcgtgt cggtgtggga gatccgcgag cactcggcgt
     8221 ccgccgtcgc ggagaccgct cccgcgggac tcaggaagga ggttgtggag ctgtgagcaa
     8281 gtcggcgttc cggctctccg tgttggtggt cggcctcgtg gctgccgcga tcctcgcgac
     8341 gcccacggcc gccctggccc acggcgaacc cccgggttcc caggacgaac cgtcggtgat
     8401 gcccgagccg gagccgcagg gtcagctcga acggctccgg gtcgttctcg acctgcccga
     8461 ggaggcatcg gtgggtgggg cgacgacgat ccgagcggtc gtcacccacc gggaggacgg
     8521 cgaccccgtc gagggactga tggtgacgtt cgaggcaccg gccacgtggg gcgaggcgct
     8581 cgccggctcg atggtgctcg gcaccgccgt gaccgatcac gacggcacag ccgtcatcgc
     8641 gaccgagatc cgagccgccg gcgacctgga ggtgacagcc cgggtcgaga gcgccgaggg
     8701 gttccgggaa gcgtccacga cggcctcgct cgaggtcgtc cggcagcgac aggtcgtcga
     8761 agacgaggtc ggactccgca tcccctggct caacctctgg gtgctggcgg gggtgatcgg
     8821 gctcgtgtgg ctcctgtacc tcaccgcggc cggccatctc agggcgatcg ctcggcaacc
     8881 ggaccagccg cccgatgcca gcccaaccca ggcgatgagc cgtcgaacgc tcctgggccg
     8941 atcgtttccc ttggccgtgc aggcaggcat cgcggcgttc ggagccggac tgatcggcat
     9001 cgtggtgcgt tcgccccgaa cccacgggaa cctgctgtca cccccgggaa ccgaagcgta
     9061 tcgccgcacc ccggtggcct tcgtcgggcg atccgattcc atgcgcgaga tgccaacacc
     9121 gttggaccgt cccgtgagct tccgggacga ggtgctgccg gtcttcatga ggttcggtgg
     9181 tccccacgtc gtccagcccg agcactcccc gccccccggc cggctacagc tcgactcgtg
     9241 ggcgcacctg atggaggccg gcgtggtggt tccgggaaag cccgaagaga gcgagctcgt
     9301 tgagcatctg ctcagcccgg gcatgcagat gccaccgtcg cgtccgccgc taccggatga
     9361 cacgatccag ctgatcgtca cctgggtcgc ccaaggtgcc caggacaact gacggaggtc
     9421 ttctcgcccg agttggatcg gatgggaggt gccgtgttga agtggcaggc aggggtgctg
     9481 cgtcggccag cttcgccagc ggtgcaggca ggactccaga tccggtcttc acagatcagg
     9541 agtcaaccga acctggggca tagcttctgg ccgaggagat gctctcccta cgcgcggcac
     9601 gaagcgcggc gtcgcgcatg cgtagcgctc ccaacgttgg ccgaatccca ccttcatctt
     9661 tcgttcctcc ccgatcgcca gccgtcggta cacgagcacg aggaccggga acatcagcag
     9721 cgtggcgaaa gtgggccact gcagcaggaa gccaactatg acgagcagga accccgcgta
     9781 ctgcgggtgc cgcacgcgcg cgtacggtcc cgtcgtggcg agggagcccg accggtgggc
     9841 ggcccagagc accctccagg ccgcggcgat cagccagaac ccagccgcga tgagcacgta
     9901 gctcgccaag tggaaggggc tgaggtgcgg atcccccccc aaccgatgag gtccgcccag
     9961 agatgaccgc cgtcgtgcgt gagacggagg gcggggaacc ggtcgcccaa ccagccggac
    10021 agcaggtaga gcgtgagcgg gaacccgtac atctcggtga agagggcgat gacgaaggcc
    10081 gagaacgctc ccatcgcctt ccagtccctt cgggtctgcg ggtggaagaa actcaccgcg
    10141 aacacgatga agagaaccga gttcacgatc actaagaacc aaagcccgta ccccgtctcg
    10201 cccgaggtgt ccatgtctca cctcttccag cgctcgtgcc gtgtcccacg tggggccggg
    10261 acccgaggga ggtcccggcc cctatccaga gcctcacggg agtcgctggc ggatcccgag
    10321 gctctggtcc gtcgcggcga tcgatttcgc cgcttcgggc tcctcgccgg tgatcgcccg
    10381 gatcgcgtcg atgttctccg ggacaacgat cgcctcgttg tggacctggt agttgaggaa
    10441 cacctcccgg ccagagactg ccagcgtgtc ctcccaaagc gcgacctccc agaggtccgc
    10501 ccgaggacga cccaggtcac gcacgatctc gatcacggag ttcggtgccg caacgccgtc
    10561 cgacacccgc acgaaggcga tccgcgaagc gtccccgaga gcatcgagga cctcgtcccg
    10621 atcgatctcg cgaggagcct cgacgacgag gaaatggacg tgggacaagt tgaacggccc
    10681 ggcagcagcg atcgttacca cgtccagctc cgggatgacc gtcttggcat ccggcccttg
    10741 atgcgaaggg atgatcgctt cgggcacgag ggtgttgatg aggccgccct ggtgcgactc
    10801 ccacgggtcg gttccccggc ggatcagcac agcgcgggtc cgggccacga gccccctctc
    10861 gcgcagagcc gagagcacgc gtacgagccc cgtcgtgttg caggagacca cgcgaaccga
    10921 gtccctatcc agagcctccg cgtagttcgc ctgcgcgacg aacgacaggc ccgtcagctc
    10981 gtgcttctca ccaccctgga acacggcctt cacgcctgcg gcctcgtagg ccggcgcgtt
    11041 ggccgcgccc acgttcttcg gcgtggcgtc gacgatgacc tccacctgtt gcaggagctc
    11101 gtccagcccc cccgatggct cgagaccagc ctgctcgaaa tctccgaccg cctgcggtgt
    11161 gctcccgaac agcggatagc tccgcgcggc cgcgagcttc acccggtagt cggtggccgt
    11221 gtcggccacg ccgatgagct ccatatcggg ctggacggtg accgcgtcgg ccactcgctt
    11281 gccgatcacc ccgtatccgt tcacgcccac cttgatggga ctcatgctgt cctcccttcc
    11341 ctcgacgacg agaaggacga taggtggggg atgtgaaggt cctgcgaaga ccgcgtgggt
    11401 ctgcgatgag gtcagatgtg tccctgagcc cgctacgatg gtccgctgtc cggacggcct
    11461 tcggtgctcg aggcgagcgg acgatgcgag aagggacgtg agcgtcgcga tcgatgccgc
    11521 gaggttcgat gcggtcgtgt tcgatctgga cggggtcctc accgacacgg cgagagtgca
    11581 ccaagcggcc tggaagctcc ttttcgacga agtgctcggg gcgatcgaag gtaccatcga
    11641 ccggcggccg ttcgaccgtg aggactaccg gagctacgtc gacggccgag cccgcgtgga
    11701 cggcgtcgaa gcggtcctca cctcgagggg tgcccggatt tcgcgcggcc ggcctgatga
    11761 tccacccgga acgaccaccg cgtgggctct ggcgaaccgg aagaacgagc acttcctcgc
    11821 cgcactggcc gcggaagggg tgcgtgcctt ccccgatgcc gcgtcgctcg tgaggagggt
    11881 gcgagcggcg aagctgggga cggccgtggt aaccgcgagc cagcatcggg cggatgtgct
    11941 ggcggcggcg cgcatcgatg agctcttcga cgtccacgtc gacggcatcg atgccgcctc
    12001 cctcggcctg gccggcaaac ccgatcccgc gatgttcctc gaggcttcgc ggcggctggg
    12061 cgttcgccca gcgcgtgcgg tggtgttgga ggacgcgctc gccggtgtgg cggcgggtcg
    12121 agcaggcagg ttcggcctcg tgatcggcgt ggaccgcacc ggcgaccgag acgcgctcga
    12181 agcggccgga gcccacgtgg tggtgacaaa cctgggagtg atcgacgtcg ggggcgcgaa
    12241 gggaggaccg ctcccgtgag ggggtgggaa ctcgtctacg aaggattcga tcccgcccac
    12301 caggggcacc gagaggtgat gtgcgcgctc ggcaacggat acatggccac ccggggcgcg
    12361 ctcgccgaat cgatcgccga cggcgtccac taccccggca cctacgtcgc cgggatctac
    12421 aaccgtctgg cctcggaggt cggcggacgc accatcgagc aggaaaccat cgtgaacctc
    12481 ccgaactggc tccctctcgc gttccggtcc gaaggaggtt cctggacggc tccggcatcc
    12541 gtgcggctcc tcgagcaccg cctggttctc gatctcctgg aaggtgtgtt gcaccgctgg
    12601 tcgcgcgtcg aagacctcga aggccggatc accgcggtgt cggagcgccg cctcgtctcg
    12661 atgtcctctg cacatctggc ggccctcgag caggtcatca cgccggagaa ttggtcgggc
    12721 gtcctcgaga tcagatcggg gctggacggg cgggtcgaga acgccaacgt ggcagaggat
    12781 cggctcctct ccaacgtgca cctggcgcca cttggggaag gcagtgagga tggcgggaca
    12841 acatggctcg aggtggagac cacacagtcc cacgtccgtg cggccgtggc cgcacgggtg
    12901 cggctccggt ccgcggacga agaggcgatc accaccggac cgcggagggt cccgcgcgaa
    12961 cccggccggg tgatcgagga agttccggtc gaggtgacgg cggggcggag ggtcgttgtg
    13021 gagaagatcg cgtcgatctg gacgtctcgt gatcgagcgc agagcgatcc gctttcggcc
    13081 gcacagtcgg acgtggccga ggagcagggg ttcgacgggc tcctcgccga ccaccgcgga
    13141 gcgtgggagc acctctggga gcggtgccgg ttcgacctcg gcgagaccga ggaggacgtc
    13201 accctcctcg tccgcctgca cgccttccac atgctgcaaa ccatctcgcc ccatgtcgcc
    13261 gacctcgatg ttggcgtgcc cgctcgcggc ctgcacgggg agggctatcg cggccacgtg
    13321 ttctgggacg agctcttcgt gttgccgctg ttcaacctgc gcatcccgga gctcgcgcgg
    13381 gcactcctgc tgtatcgggc ccggcggctg ccggcggcgc tgcgggcagg gagggccatg
    13441 ggcatgccgg gggctcggtt cccgtggcag agcggctcgg acggccgcga acttacgccc
    13501 gcgcagctgt ggaacccgcg ttcacagcga tgggtcgccg acaactcccg gctgcagcac
    13561 cacgtgagcc tggccatcgc gtggaacctg tggcagtact accaggccac cggcgacgag
    13621 cggttccttg ccgagtacgg catcgagatg ttggtgcaga tcgcacgctt ctgggctgga
    13681 ctggcgatcc gcgagccgcg agacggccgc ttcgatatcc gcggcgtgat gggacccgac
    13741 gagttccacg acggatatcc ggaccgccca ggtgcgggca tcgacaacaa cgcctacacg
    13801 aacgtcctcg cgagttggac gctccaacgg gccgtggagg tgcgccacct cctctgtgag
    13861 cagccggacc gccatctctg ggaccgcctg ggagtgacgg acagcgaggt cgagtcgtgg
    13921 ggacacatca gccagcgtct cagggtgccc ttccacgacg ggatggtgag ccagttccag
    13981 ggctgggagc ggctggccga gctcgactgg gaggggtacc ggtcgcgcta cgggaacatc
    14041 ggtcggctcg atctgatcct cgagtccgaa ggcgacagca cgaaccgcta caagctcgcc
    14101 aagcaggccg acgcactcat gctcttctac ctgttctcgg ccgacgagct cggcgcgctc
    14161 ttcgatcacc tcggctacga gttcgatccc cagaccatcc cggccaccgt ggagtactac
    14221 atcgcccgca ccacccacgg ctcgacgctg agccacctcg ttcacgcctg ggtggttgcg
    14281 aggagcaacc ggtccgggtc gtgggaggtc ttccgtgagt cgctcgtggc tgacctcgac
    14341 gacacccaag gcggtaccac cgccgaaggc atccatctgg gggccatggg cggggggctc
    14401 gacgccctcc agcgctgtta cacgggcctc gagatgcgcg gcgatgccct ccggctcgcc
    14461 ccacgactgc cggacgggct ccggtcgctc ggcttcgaca tgacctaccg cggtcaccag
    14521 ttgaccctgt ccgtggatcc cgagcggatg aagatcaccg cccatccctg ctctgccgcc
    14581 ccggtcgacc tcatggtggg agggcgtgcg atcacgctcg ccgccgacca gaccgtggag
    14641 gttcccgtgc gcgaggacgg ggtcgaagga gcctgatcgc acgctgaggg aactcgttgc
    14701 gaagccgccc gagatcaaac gaactggcta ggatcgggct gggtgcctgc cggccaaccg
    14761 gggtatccgt cgcccgggta ttcccagggt gtcccgtagc agtcgtcgaa cctcggaacc
    14821 ggccgagcgt tcatcctcgg atcccgccgg aaatcgaaca cgtgctcgaa attgtgggtc
    14881 cgctcgatac gcggagtgag atacgggagt ccccagttgt cctcgatgaa cctcagaggc
    14941 gttgagaact ctccctcggc gtcgtcgatg tagccctcct tcgcgaacgg ggagaccacg
    15001 agcatcggga cgcggaagcc gagtccgagt ggatcgatct cgggcggggg gacatggtcg
    15061 tagagaccac cccactcatc ccacgtgagg aagatcaccg tatgtctcca ggcggcgctc
    15121 cgcatcaccg cgttcacgac atcggcaacc cagttctgtc cgaagcagac gctttgcggc
    15181 gggtggtccg agagctcgaa cctcggggtg atccaggtca cggacggtaa gcgatcggcg
    15241 gcgatgtcct cgagcagatc gtcgacgggg cggatcgtgt ctccgggccg ccacagatcg
    15301 gtatggaaga cgcccgcgat cccgttcaac gcattccaga agtacccggt ctggtggcgg
    15361 ctcgccgagt agtaagccca gctcacggaa gctttctcga gctgctcagg caccgtcggg
    15421 aggctgaaac acgtgtcgtg cttcgtgaga ttgccttcct cgtccctcac gaagacgaaa
    15481 acgtcgtcac cgacagcatc acatccccag cttttgaaac gccggttgcc gccatcgatc
    15541 cggacctcga tgttctcggg gttgtcgatg gcgccgccag actggccggc gacgaagaag
    15601 aagtggttcg ggtacgaggg tcctccgacc gaagcgaaga agttgtcgca gagcacgtaa
    15661 tccttcgcga gttgccagta ggccgggacc ttgtcgctgt cgaacgtggt gtacgcccaa
    15721 ggatcgccga aacgcccgtt gccgaagccg tcgtacgcac ccccgttggc gtcgttcaga
    15781 tgagcggcac ggtcgtgtgg caggtcgccc gggagccagt gcggggatgg cagcaaacga
    15841 acctcccggc cgttcttcac accgacgagc gaaccgttcg ctccagggaa cctcccgaag
    15901 aggttgtcga agctccggtt ctccatcatc aggtagatgg cccgtttgat cggccagcga
    15961 gtctcggtcg cgacgacgga tcccgtcacg ttcggcgcga caaccggcag cttacgtcca
    16021 ccccccgtgc acgcgtacag accggcgcct gcgagcgcga gcccagacgc cttcaggaat
    16081 ctccggcgtg agagggatag cgagcggtag tgctccgagc ggtcgcaccc gcgctcctcg
    16141 ctcatctcaa gctcggccgg agccgggacg atgggtgttg gctgtcactc gccgacccga
    16201 ctcggatggg acctcgaagc ctcggcgtca acttcctccc cgccctcgcg ggtcggcagc
    16261 cgcaagcggt tcagcatgag cgcattcact gcgacgatca aactcgaccc ggacatcgag
    16321 agcgccgcga tctccggccg cagcgtcagc ccgatgctcg gctcgaatac ccctgcggcg
    16381 atcggcagcg cgatcgcgtt gtagccgatc gcccacccca ggttctgccg catcttgcgc
    16441 agggttccgc gcccgatact cagagccgtg ggaacatcga gcgggtcaga gcgcatgagc
    16501 acgatgtcag ccgtctcgat cgcgacgtcg gtgcccgccc cgatcgcgat gccgagatcg
    16561 gcctgagcga gcgcaggggc gtcgttcact ccgtcgccaa ccatcgccac cttccgaccc
    16621 tcccgctgaa gctcggacac cttgccggcc ttgtccccgg ggagcagttc cgcgaggacg
    16681 gtgctcacgc ccaactccgc tgcgatccga cgggcggtcg cctcgttgtc cccggtcagc
    16741 atcacgaccg cgaccccgga ctccgcgagg gcctccaccg cggcacggga cgttggacgg
    16801 accgcgtcgg cgatcccgat caaaccggcc gtccggccgt ccacggcggc gaccaccgcg
    16861 gtcatccctc ccgaggcaac cttctgacgt cgctcttcga gggggcccat ggcgatgccc
    16921 tcgcgatcca tgagccgtcg ccatcgactc accgaagcgg tcgaggggct ccggcgctcc
    16981 cagcagcgct cgcttgagag cgatctccgt gaggtcctcg gccgagtacg tcctcccgcc
    17041 cgacgagtag ctcatctcgg agaagaggtt gctctccggc tgatccgtcg cgagcgagag
    17101 acgaacctcg gccgccgtgc tagccgtcgt cgttacctcg acgtcttcga tgcggctccg
    17161 tcggacgcgt cctgagtaga tgaacgtccc ttcgtgctcc ctcccccatc gatccgagcg
    17221 catcgcctcg agtcgcgcca agacctccct gtccctcacc cgcgcgacca cctcggctcg
    17281 actcgctccc tcgcttatcc ggcgtgctcg gaacacgacc gttccgagct tcgcccacgg
    17341 cgccacgtcc tcggcagcga tccgtcggag gcgtcggcca acgtccctgg ccagctcctc
    17401 cggagtggtg aaccgcccgg tggtgtggaa ggtccggacc tcctcgagga acgactcctc
    17461 gtgcccttcg cggtcctcca catccttggc ccacagcgcc acccggagac cctgctcctc
    17521 cgcggccagg tactccgcat gcgtggccga gtaccgggac gggagctgcc gtccgtaccg
    17581 tcggccgagg atcccgacgt agaccgatga cgaggcgatt tcgtggacgt acgcggcctg
    17641 gtcgtcgtcc tcacggccac cgaaccgctc gaagagcact ggctcgaacc cgaggtcctc
    17701 gatgtggtcc gccaccgcac ggcgttcttc gctcagctcc tccatcacgc tggagatgaa
    17761 gacacgttgc tcgcgcgccc acgccctgaa ttcctcttcg gagatcggtt ccgtcgcggc
    17821 gccccggtcc acgaccaatc gaccgccctc ttcatccatg ttcccgaaaa tacgacccac
    17881 ggccgcgcta gcattcgaaa ggccagacga cggggtcatg ggatggggcc gaacccgatc
    17941 gacatgaccg cgtcggccac tgcgcgggcc gcttctcgcg cgtcgtcccg gtccccgcgg
    18001 tagtcgtttc gggccacggg cagatggatc gtcgcgctga agggcggcag atcccggttg
    18061 agatcgatgt cgatctcctc cagcttgacg cccagctccc cgccgatcca ggcacggccc
    18121 gtcgaggaca cacgcgccac atcgtggatg cttacggcgg agtaggcgat cgagaacagg
    18181 ttgtccgttc gttcgaggag cgtcagctcg attccgacga gcgagctccg aagtaccacg
    18241 agcactggag cgacgcgagc gtcgtcctcc cacgagtagt agacggccag gggctcttca
    18301 tccttgggca agcagtccgt gattaggtgc ccgatccacc tcgggtaggt gcccgtgggc
    18361 tcttccattc gctgacgcca ggatacacgt tggcgaacac atgtttgagt ccgacgcccc
    18421 cggcgccgcc ttttgcgcct ccgccggacg cggcgcaacc ggcgcagcgg cgcaatcgac
    18481 accgtttcct aaaccgtgtg cggaggttcg attcctcccg ggggcacatt gtgatgtgtc
    18541 ggaccattgt gaagttccgg cacctctgtc ccacatcggc tccggcacct gtgtcccacc
    18601 ctcatgcccg gctccggctc ccgggcgaca ccggctccgg cacctgtgtc ccacccgctc
    18661 cacgtgccac tcgagcctga tcttca
//
LOCUS       DASUYD010000250         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1526304, whole genome shotgun sequence.
ACCESSION   DASUYD010000250 DASUYD010000000
VERSION     DASUYD010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526304"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF195_13160"
     CDS             <1..190
                     /locus_tag="VF195_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002527307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX6581800.1"
                     /translation="PLEGHESLEAILSDGTGEVTVVWMGRRAIRGLSLGTRLVVDGLL
                     AEQRSEKRMVNPSFEFAA"
     gene            263..814
                     /locus_tag="VF195_13165"
     CDS             263..814
                     /locus_tag="VF195_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581801.1"
                     /translation="MWKAWRGTIVTVVAVIAIGSLASASPSLRAPRGPHESTGPSGVT
                     GLSEPAGPTGSELEPVEEKEETADPVETSSNGADFSACEGLTGLDNAICRHEALLVVH
                     PDNRGLRYSLGHLRENQAKHEGSASEHDPGDDPSQDAATGGSSSGHGQSGEPHGNGGG
                     NDHGNASGNDHGNAGGNGNGKGQ"
     gene            892..2079
                     /locus_tag="VF195_13170"
     CDS             892..2079
                     /locus_tag="VF195_13170"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX6581802.1"
                     /translation="MAEERRGLAVETESVEALVGREVVEPTVATEWTQEEARRRKAAA
                     LRIYLGAAPGVGKTYAMLSEGRRRRDRGTDVVVGFVATYGRPQTIAMLEGLEIVPMQI
                     LGYRGRGFEEMDVDALIERNPRVALIDELAHTNIPGARHAKRWEDGVESLAAGIEVIT
                     TVNIQHLESLNDVIANVTGIRQMETIPDWLFDLADQVELVDMSPYALRRRIAHGNVYP
                     DARKAELALQRFFTMENLTALRELALLKVANQVDDTLIGRWQSQGAATDVRERVLVCV
                     ERPGEISDTLIRRASRIAQRARGELLVLHVRQGEGATDPAWLEEVGSLVRDLGGTFDV
                     IDSETPVDAVLGYAYRHSVTQIVVGESLRSRWQELTRGSFVTRLIRAASNLDIHVISR
                     RER"
     gene            complement(2057..2380)
                     /locus_tag="VF195_13175"
     CDS             complement(2057..2380)
                     /locus_tag="VF195_13175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581803.1"
                     /translation="MSDTRTWVVVGARPRSSAIDVLGDVPAGGEAVVFVLGLHPTRAQ
                     QRLTEDALELAAERRFTLTAELISGPSQLHERSPEATTVRIVAGRGERRKWHLEDPFT
                     APGAR"
     gene            complement(2386..2964)
                     /gene="kdpC"
                     /locus_tag="VF195_13180"
     CDS             complement(2386..2964)
                     /gene="kdpC"
                     /locus_tag="VF195_13180"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020142626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX6581804.1"
                     /translation="MIANALRALRAVVVLALLTGLAYPLVITGIAQLTQSDKADGSLI
                     RVDGHVVGSSSIGQAWEGDEWFHGRPSAVDYDASTSSGSNLGPNSQELSDQFVERVEA
                     ILKLEGPYTADLTVADIPIDLLTASASGLDPHISVEAAALQAPRIAEVRGLSLEQVRA
                     LIDDHTEGPVLGFLGKERVNVLELNLALAQAA"
     gene            complement(2961..>3432)
                     /locus_tag="VF195_13185"
     CDS             complement(2961..>3432)
                     /locus_tag="VF195_13185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005484230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit B"
                     /protein_id="HEX6581805.1"
                     /translation="AQADVGVAMNTGTTAAKEAGNMVDLDSNPTKLMEIVEIGKQLLI
                     TRGALTTFSIANDVAKYFAIIPAMFAGILPALDTLNLMDLATPESAILSAVIFNALII
                     VALVPLALRGVRYWPIGADGLLRRNLLIYGLGGIVVPFIGIKAIDLLLEVVKFP"
BASE COUNT          581 a         1126 c         1217 g          508 t
ORIGIN      
        1 gccgctcgag ggccacgagt cgttggaggc catcctgtcg gacgggacgg gcgaggtcac
       61 ggtcgtgtgg atgggacgcc gagcgatccg cgggctcagc ctggggaccc gcctggtggt
      121 ggacggcctg ctcgccgaac aacgctcgga gaagcggatg gtgaacccca gcttcgagtt
      181 cgcggcctag gcccgcgggg ggaggtcgaa aggcgggtca agttcgggga ggcatccttc
      241 cgataccact catatggatc gtgtgtggaa agcgtggcga ggcacgatcg tcacggtcgt
      301 cgcggtgatc gccatcggct ctctggcttc ggccagcccc tccttgcggg ctccacgcgg
      361 accgcacgag tcgaccggtc cgtcgggcgt cacggggttg agcgaacccg ccggacccac
      421 cggttcggag ctcgagcccg tcgaggagaa ggaggagacg gccgaccccg tcgagacgtc
      481 ctcgaacggg gcggacttca gcgcctgcga gggcctgacc ggactcgaca acgcgatctg
      541 ccgtcacgag gcgctgctcg tcgtccatcc ggacaaccgg ggattgcggt actcgctcgg
      601 tcaccttcgg gagaaccagg ccaaacacga gggctcagcg agtgagcacg atcccggcga
      661 cgacccgtcc caagacgctg cgaccggcgg gagctcgtcc ggccacggcc agagcgggga
      721 gccccacggc aacggcggcg gcaacgacca cggcaacgcc agcggcaacg accatggcaa
      781 cgccggcggt aacgggaacg gcaagggtca gtagccgacc cctccccgat ccactcgccc
      841 tcgagcgaga actcgggggc gaccgccctc cgtgtcaggc atcatctctg tatggcggag
      901 gaacgaaggg gcctcgcggt cgagaccgag tcggtcgaag ccctggtcgg acgggaagtc
      961 gtcgaaccca ccgtcgcgac cgagtggacg caggaggaag cgcggcgccg taaggccgca
     1021 gcgctgcgga tctacctggg cgccgcgccg ggggtcggca agacctatgc gatgctgtcc
     1081 gagggacgcc gccggcgcga tcgtggtacc gacgtcgtcg tcggtttcgt ggcgacctac
     1141 gggagaccgc agacgatcgc gatgctcgaa ggtctcgaga tcgtgccgat gcagatcctc
     1201 gggtaccgcg gccgggggtt cgaggagatg gacgtcgacg cgctgatcga acgcaaccca
     1261 cgcgtcgcgc tgatcgacga gctggcgcac acgaacatcc cgggggcgag gcacgccaag
     1321 cgctgggagg acggcgtcga gtcgctcgcc gccgggatcg aggtgatcac gacggtcaac
     1381 atccagcacc tcgagagcct gaacgacgtc atcgcgaacg tcaccgggat ccggcagatg
     1441 gagaccatcc ccgattggtt gttcgatctc gcggaccagg tggagctcgt cgacatgtcg
     1501 ccgtacgcgc tccgacgccg gatcgcgcac gggaacgtct acccggatgc ccggaaggcc
     1561 gagctcgccc tgcagcggtt cttcacgatg gagaacctga cggcactgcg cgagctggcg
     1621 ctgctgaagg tggcgaacca ggtcgacgac acgctgatcg gccgatggca gagccaggga
     1681 gcggccaccg atgtgcgcga gcgcgtgctc gtttgcgtcg aacgccccgg tgagatctcg
     1741 gacacgctga tccgaagggc gtcgcgcatc gcgcagcgag cccggggcga gctcctcgtg
     1801 ctgcacgttc ggcagggcga gggcgccacc gatccggctt ggctcgagga ggtcggatcg
     1861 ctcgtgcgcg acctaggtgg aacgttcgac gtgatcgact ccgagacacc cgtcgacgcg
     1921 gtactcgggt atgcctaccg gcactccgtc acgcagatcg tcgtcggcga gtccctccgg
     1981 tcgcgctggc aggagctgac ccgtgggtcc ttcgtcaccc gcctgatccg ggcggcgtcg
     2041 aacctcgaca tccacgtcat ctcgcgccgg gagcggtgaa cggatcctcg aggtgccact
     2101 tgcgacgttc gccccgtccc gccacgatcc gaacggtcgt ggcttcgggc gatcgctcgt
     2161 gcagttgcga cgggccggag atcaactcgg ccgtcagcgt gaagcgacgc tcggcggcga
     2221 gctcgagggc gtcctccgtc aggcgttgct gtgcgcgggt cgggtgcagg cccagcacga
     2281 agacaacggc ctcgcctccg gcgggcacgt cacccagcac gtcgatcgcg ctcgagcgag
     2341 ggcgcgctcc gacgaccacc cacgttctcg tgtccgacac cgtcctcatg ccgcctgcgc
     2401 gagcgcgagg ttgagctcga ggacgttcac gcgctccttg cccaggaacc ccaggaccgg
     2461 cccctcggtg tggtcgtcga tcagcgccct gacctgctcg agcgacagac cccgcacctc
     2521 ggcgatccgt ggtgcttgga gcgccgcggc ttcaaccgag atgtgcgggt ccagaccgct
     2581 cgccgaggcg gtcagcaggt cgatcgggat gtccgcgacg gtcagatccg cggtgtaggg
     2641 gccctccaac ttcaggatcg cttccacccg ctccacgaac tggtccgaga gctcctgaga
     2701 gttcggaccg aggttcgagc cggaggacgt ggaggcgtcg tagtcgacgg cggagggacg
     2761 accgtggaac cactcgtcgc cctcccacgc ctgtccgatg gacgagctcc cgacgacgtg
     2821 gccgtcgacg cggatcaagg acccgtcggc cttgtcggac tgcgtcagct gcgcgatccc
     2881 ggtgatgacg aggggatacg cgagaccggt gagcaatgcg aggacgacca cggcgcggag
     2941 ggctcggagc gcgttggcga tcacgggaac ttcaccacct cgaggagtag atcgatggcc
     3001 ttgatgccga tgaacggaac gacgatccct ccgagtccgt agatcaggag gttgcggcgc
     3061 agcaggccgt cggcaccgat cggccagtag cgcacgcctc ggagtgcgag cggcacgagg
     3121 gccacgatga tcagcgcgtt gaagatcact gcggacaaga tcgccgactc cggcgtcgcg
     3181 agatccatga ggttcagcgt gtcgagcgcc ggcaggatcc cggcgaacat cgccgggatg
     3241 atcgcgaagt acttcgcgac gtcgttcgcg atcgagaagg tcgtgagcgc tccgcgcgtg
     3301 atcagcagct gcttgccgat ctcgacgatc tccatgagct tcgtcgggtt cgagtcgagg
     3361 tcgaccatgt tgccggcctc cttggccgcg gtcgtcccgg tgttcatggc cacgccgacg
     3421 tcggcctggg cg
//
LOCUS       DASUYD010000251         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_643702, whole genome shotgun sequence.
ACCESSION   DASUYD010000251 DASUYD010000000
VERSION     DASUYD010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643702"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VF195_13190"
     CDS             <1..460
                     /locus_tag="VF195_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581806.1"
                     /translation="GDRGYYSAPALSPDGTDLYVVYNAFTTPFRSDTTSVRGLVGVVL
                     HADVGPDGAPSGLTEVHRGATGDPRGSSQNNIVIEFLGDYVYASATNEYAVAVWQDAR
                     NAADCPAVDAFRADFQITRSLAGRPAIQQACPATFGNTDIFAWSGLDPTP"
     gene            618..>2152
                     /locus_tag="VF195_13195"
     CDS             618..>2152
                     /locus_tag="VF195_13195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581807.1"
                     /translation="MNARRLASAVLVLVSVITVTSAVSTAQTPTTVAEIQGASHISPL
                     VGQSVTGVEGVVTARRTAGGRGVWIQDPAPDADIATSDGVFVFLNAAPVAQIGDLVGV
                     NGTVSEFRPGGDPDNLTITQITSSNANVTILSSGNPLPAPIVLGPGGRVPPTENIDDD
                     GAGNVETGGTFDAVNDAIDFYESLEGTRLQANDGAVVGATRSFGEISLLPDDGSWATG
                     LRTARGGILLGGYEDPNPERVIVDDEILRDLAPAPRPAKAMPDMNVGDSLTSPVIGPL
                     DYSFSNYKIQATSTPLFSSGGLVRETTAPTTDHEVSVATFNVENLAGTDPQAKYDHLA
                     ELIVNNLRAPDIIGIEEVQDNDGVAGGTGSSVVDASVAWTRLIQAIQATGGPTYQHRQ
                     IDPVDDAEGGAPGGNIRVGFLFRTDRDVEFIDRSGGGPTTETTVVAHPSGPRLSSSPG
                     RIGTQSPAFDDTRKSLVGEFRARGKKLFVVVNHFSSKTDDQPLFGPAQPPTRFSEVAR
                     HGQA"
BASE COUNT          379 a          751 c          711 g          311 t
ORIGIN      
        1 cggggaccgg gggtactact cggcaccggc gctctctccc gacgggacag acctgtacgt
       61 cgtgtacaac gccttcacga ctccgttccg tagcgacacg acgagcgtgc gtggcctcgt
      121 cggcgtggtg ctgcacgccg acgtcggtcc cgacggagcg ccgtccggct tgacggaggt
      181 gcatcgagga gcaacagggg acccgcgggg ctcatcccag aacaacatcg tgatcgagtt
      241 cctgggggat tacgtgtacg cctccgccac gaacgagtat gcggtcgcgg tgtggcagga
      301 tgcgcgaaat gcggctgact gtccggcggt tgatgccttc cgcgccgact tccaaatcac
      361 acggtcgttg gcaggacggc cagcgatcca acaggcctgt ccggcgacgt tcggaaacac
      421 ggacatcttc gcttggtctg ggctggaccc gacgccatag atccgcgtag gccaaaaagg
      481 atggaccgag gggccggcag cccggccctt cagtccgcct ggatcgcgga gactgcgggg
      541 acaccgacat ccggtacgcg gacccggacg gcgagggccg ctacgtcgcc aactgaacga
      601 cgggagggat gaccacgatg aacgctcgac gcctcgcttc ggctgtactg gtcctggtga
      661 gcgtgatcac cgtgacatcg gcggtctcca cggcacagac gccgacgacc gtcgcggaga
      721 tccagggcgc ttcacacatc tcgcccctcg tcgggcagag cgtcaccggc gtggagggcg
      781 tcgtcacggc gcgacgcacg gcgggcgggc gaggcgtctg gatccaggat ccggctcccg
      841 acgcggacat cgcgacctcg gacggggtgt tcgtcttcct caacgccgcg cccgtggccc
      901 agatcggtga tctggtggga gtcaatggga cggtgagcga gttccgcccc ggcggcgatc
      961 ccgacaacct gaccatcacc cagatcacct cctcgaacgc gaacgtgacc attctgtcga
     1021 gcgggaaccc gctgcccgcg ccgatcgtgc tcgggccagg gggccgtgtc ccgccgaccg
     1081 agaacatcga cgacgacggc gccggcaacg tggagaccgg gggaacgttc gacgccgtga
     1141 acgatgcgat cgacttctac gagagccttg agggcacgag gctccaggcg aacgacgggg
     1201 ccgtggtggg cgcgacccgc agcttcggcg agatctcgct cctgcccgac gacggctcct
     1261 gggcgaccgg gctccgcacc gcgcgaggcg ggatcctcct cggcggctac gaggacccga
     1321 acccggagcg ggtcatcgtc gacgacgaga tccttcgcga tctcgccccg gcaccacggc
     1381 cggccaaggc gatgcccgac atgaacgtcg gcgactcgtt gacctcgccg gtcatcgggc
     1441 ccttggacta cagcttctcg aactacaaga tccaggcaac ctcgacaccg ctattctcgt
     1501 cgggcgggct cgtccgcgaa acgacggcgc cgacgacgga ccacgaggtg tcggtggcga
     1561 ccttcaacgt cgagaacctc gccggcaccg atccacaggc caagtacgac cacctcgccg
     1621 agctgatcgt gaacaacctc cgggcacccg acatcatcgg catcgaggag gtgcaggaca
     1681 acgacggcgt cgccggcggc accggcagct ccgtcgtgga cgcgtctgtg gcgtggaccc
     1741 gccttatcca ggcgatccag gccaccggtg gccccaccta ccagcaccgc cagatcgatc
     1801 ccgtcgacga cgccgagggc ggcgcccccg gtgggaacat ccgcgtgggc ttcctcttcc
     1861 gaaccgatcg cgatgtcgag ttcatcgacc ggtccggtgg gggacccacg accgagacga
     1921 cggtcgtcgc gcacccgtcc gggccgcgcc tgtcctcgag tcccggccgg atcggcacac
     1981 aatcaccggc gttcgacgac acacgaaagt ccctcgtcgg cgagttccga gctcgcggca
     2041 agaagctgtt cgtcgtcgtc aaccacttca gctcgaagac cgacgaccag ccgctcttcg
     2101 gtccggcaca gccaccgacc cgtttcagcg aggtcgcacg ccacggccag gc
//
LOCUS       DASUYD010000252         3797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_203027, whole genome shotgun sequence.
ACCESSION   DASUYD010000252 DASUYD010000000
VERSION     DASUYD010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3797
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203027"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..73)
                     /locus_tag="VF195_13200"
     tRNA            complement(3..73)
                     /locus_tag="VF195_13200"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39..41),aa:Cys,seq:gca)
     gene            300..473
                     /locus_tag="VF195_13205"
     CDS             300..473
                     /locus_tag="VF195_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581808.1"
                     /translation="MLFLIGAIALALVALGTSWGTIGTVVILITATVWLTLAFRPVEV
                     GALVTSLTRRRFE"
     gene            969..1502
                     /locus_tag="VF195_13210"
     CDS             969..1502
                     /locus_tag="VF195_13210"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6581809.1"
                     /translation="MPNTTSGLETFTDGWAHYQGLVLDAIRDLTPEQLGSRTASHQWA
                     VWQLAGHVAGSRAYWFHDVLGEGDPATRDLFRVASTTVPALPLEDAGWEDDETHPRSA
                     AEIVEAFDLTWAMIDDCLRRWTPDDLTVGFSRERRSGTETFTRQWVIWHLVEHDLHHG
                     GEISQILGSHGVPALNL"
     gene            complement(1527..1865)
                     /locus_tag="VF195_13215"
     CDS             complement(1527..1865)
                     /locus_tag="VF195_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581810.1"
                     /translation="MAGTHGDASLVVELAKLGAMNGLGEAVGKIFADDFDPDAADLSD
                     PSVRIVLGWNETVATLVKNDLLSRDLVYDWLWVAGTWDRVGPAALRAREAADVAVLYE
                     NFEALAAGQR"
     gene            complement(1916..2608)
                     /locus_tag="VF195_13220"
     CDS             complement(1916..2608)
                     /locus_tag="VF195_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX6581811.1"
                     /translation="MLIRLFRGVIREGAHDRLLRGLHDHVLPRLEAHPGVTSTTLAVA
                     MEGSPDEYLVESHWRGVDDLIRFAGDEWRTPRVEPAEEELLTSVSAHHFVTQGPVVPS
                     AAKLTTVPAAIRLGDVQIDGARLRVAWNGSAVHLPPREMAAMLALARDVGAPVASGEL
                     ARRIWPGSAMVTPYDVRRVVHQLRVLLRSNGTPIHIRNVHGVGYGLELERPDETASIP
                     DTTRTSPPGHRV"
     gene            complement(2929..>3797)
                     /locus_tag="VF195_13225"
     CDS             complement(2929..>3797)
                     /locus_tag="VF195_13225"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581812.1"
                     /translation="LAACAPQEYHADTPAQYQSAAGCAQVNRDHLEAAAGYANAYLTV
                     ATGDPAFPPWWEGGSTDEHPEWTLNDPYLGEGFEGAVTFEVAERLGFSEDRVRFVPIG
                     FTESFAPGPKGFDFVLQQISFLPERAESVELSEGYYDVDQALVSVRGSPIADASTLEE
                     LKDATLGAPIDTTSLNVIEDTIRPTERPAVFDDLSAAVEDLEHGQVDGIVADLPSASF
                     ITATQVPEGVVVGRLPSVGSQEYFAMAFEKGSPLVECVNLALAEMKDDGSLEAIRREW
                     LEVTADAPLIRG"
BASE COUNT          606 a         1317 c         1249 g          625 t
ORIGIN      
        1 cgaggcgggg accggaatcg aaccggtgtg cgaggctttg caggcctctg cctaaccact
       61 cggccacccc gccggacgag gggccagtgt agccgcggct cgcggcaggc ttgcccctcg
      121 aagggtgcag ggtacagaaa ctccggagat cggcccgacg ggaacatccg accacctggt
      181 gggtgttcac acagtgttca cgcagccccg gcctcacagg ctccttcggt gggtagcctc
      241 gaagagcaag gaaggaacca tcgccgcgcc acagcgcggt ctcgaggaaa ggaagcacga
      301 tgctgttctt gatcggagcc atcgcactgg cgctcgtcgc cctcggcacg agctggggca
      361 cgatcggcac cgtcgtgatc ctgatcaccg cgacggtatg gctgacgctc gcgttccgcc
      421 cggtcgaggt gggggcgttg gtcacgagcc tcacgcgccg gcgcttcgag taaccagcag
      481 ggatcccgca cgatcgggag gccggcccga tgggccggcc ttcgtcgtgc ccgtccatcg
      541 ctgatcctct ccgcttcgag ttgaggtccg atcggctcgc agggaggatg ccggggtgga
      601 gagctcccgg agcaaggcga tcgtgctcgc ggcggcgctc tcgttgcccg ctcgcggggc
      661 tcgcactgct ccctcgcggt ccctgatctg gacgtgcact gggaacatca cccctctcat
      721 ttctggctcg tcctcagcgt cgccttcatc gacacagtgc tcgggatcgc gatgagcgag
      781 gcggccactc gccgaggcga tgccggcgct cggttccacg ctcgttgaag ggcaggacgg
      841 agaccatcga cgtgtacgaa ctgctcgacg tggtttcgtg acagactcag gaggccggcc
      901 cgatccgacg gctgacaggt tccgccctca ggtgacctga ggggacgtgg gcgcctacca
      961 tgcgccgcat gccgaacacg acctcaggac tggagacctt caccgacggc tgggcccact
     1021 accaaggcct cgtcctcgac gcgatccgtg atctcacccc cgagcagctc ggatcgcgga
     1081 ccgcttccca ccaatgggcc gtctggcagc tcgccggtca cgtggcgggg tcccgcgcct
     1141 actggttcca tgacgtcctc ggcgagggtg atcccgccac tcgtgacctt ttccgcgtcg
     1201 cgagcacgac cgttcccgcc ctgccgctgg aggacgccgg ttgggaggac gacgagactc
     1261 accctcggag cgcggccgag atcgtcgagg cattcgatct gacctgggcc atgatcgacg
     1321 actgcctccg gcggtggacg cccgatgacc tcaccgtcgg gttctctcgc gagcgccgct
     1381 cgggaacgga gacgttcacg cgtcagtggg tgatctggca cctcgtcgag cacgacctcc
     1441 accacggcgg tgagatctca cagatcctcg gaagccacgg cgtccccgcg ctcaacctct
     1501 gacgctccga tctcggcgcc ctcggttcat cgctgccctg ctgccagcgc ctcgaagttc
     1561 tcgtagagaa cggcaacatc ggccgcctcg cgggctcgga gggcggcggg gccgacccgg
     1621 tcccaggtgc ccgccaccca caaccagtcg tagacgaggt ctcggctcag cagatcgttc
     1681 ttcacgagcg tcgccaccgt ttcgttccag ccgaggacga tgcgaaccga cgggtcgctg
     1741 aggtcggctg cgtcgggatc gaagtcatcg gcgaagatct tccccaccgc ctctccaagc
     1801 ccgttcatcg ctcccaactt ggcgagctcc acgacgagcg aggcatctcc atgggtcccg
     1861 gccatcggtc cctccttccc tctccggcga ccgcaccccg gtcgctcacc gatttctaca
     1921 cccgatgtcc tgggggagat gtcctggttg tgtccgggat cgacgcggtt tcgtcgggcc
     1981 gctcgagctc cagcccgtac ccgacgccat gcacgttgcg gatatggatg ggcgtaccgt
     2041 tcgagcggag gagcacgcgc agctgatgga cgacccggcg aacgtcgtac ggcgtgacca
     2101 tggcgctccc gggccagatt cgccgcgcga gctcgccgga ggcgaccgga gcccccacgt
     2161 ctcgcgcgag cgcgagcatc gcggccatct cccgtggcgg cagatgcacg gccgatccgt
     2221 tccatgcgac ccgaaggcgg gcaccgtcga tctgcacgtc gcccagacgg atcgccgcag
     2281 gcaccgtcgt gagcttcgcc gccgacggaa caacgggtcc ctgcgtcacg aagtggtgag
     2341 cggagacgga ggtgaggagc tcctcttccg cgggctcgac acggggagtc ctccactcgt
     2401 cgccggcgaa ccggatcagg tcatcgacac cacgccaatg ggattccacc aggtactcgt
     2461 cgggtgagcc ctccatcgcg accgcgagcg tcgtggaggt taccccgggg tgtgcctcga
     2521 ggcgtggaag gacgtggtcg tggagcccac ggaggagtcg gtcgtgcgct ccctcccgga
     2581 tcaccccccg gaacagcctg atcagcacgg aggcacgcta tcgggagaca ccgacagggg
     2641 gttccgccga cccggctccc cgcagtggtc ggcagatccg gtcgaagctg accgtatggg
     2701 agcttggagc cgacgatgga ccccaacccg tctggcagct gtatcgcgga cgtgatcgag
     2761 aggcccgtcc gatacgtcct tacacagggg acggtggaat ccggggacgt cgcgactctg
     2821 gttggatgac tccgacaatg ggatcgacgt gaggtcccgt gtcccctggg atgactccac
     2881 gccgcgatgc caacgtgagc gtctcctctg cccggtcatc gctcgaggtc agccccggat
     2941 cagtggcgcg tcggccgtca cctcgagcca ctccctgcgg atcgcctcga gcgagccatc
     3001 gtccttcatc tccgcgagtg cgaggttcac gcactcgacg agcgggctcc ccttctcgaa
     3061 agccatcgcg aagtactctt gcgagccgac gctcgggagg cggccgacga ccacgccttc
     3121 cgggacctgc gtcgcggtga tgaaggatgc actcggaagg tccgccacga tgccatcgac
     3181 ctgtccatgc tccaggtctt ccaccgcggc gctgagatcg tcgaagacgg ccggccgctc
     3241 ggtcggccgg atcgtgtcct cgatgacgtt gaggctcgtg gtatcgatcg gtgcccccag
     3301 cgtggcgtcc ttcagctcct cgagcgttga ggcgtcggcg atcggcgagc cccgaaccga
     3361 cacgagcgcc tgatccacgt cgtagtaccc ctcgctgagc tccacgctct ccgcgcgctc
     3421 cggcagaaag gagatctgtt ggaggacgaa gtcgaacccc ttcggaccgg gcgcgaacga
     3481 ctccgtgaac ccgattggga cgaacctgac cctgtcctcg gagaaaccga ggcgctcggc
     3541 gacctcgaac gtcaccgcgc cctcgaagcc ctcacccaag tacggatcgt tcaacgtcca
     3601 ctcgggatgc tcgtcggtgc tgcccccctc ccaccagggc gggaaagcag ggtcgccggt
     3661 agccacggtc aggtaggcgt tggcgtatcc ggcggcggct tcgaggtggt ctcggttcac
     3721 ctgggcgcag ccggcggcgc tctggtactg agcgggcgta tcggcgtggt actcctgcgg
     3781 ggcgcacgcg gccagca
//
LOCUS       DASUYD010000253        15447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_644446, whole genome shotgun sequence.
ACCESSION   DASUYD010000253 DASUYD010000000
VERSION     DASUYD010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 15447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15447
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644446"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..1573
                     /gene="rny"
                     /locus_tag="VF195_13230"
     CDS             41..1573
                     /gene="rny"
                     /locus_tag="VF195_13230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX6581813.1"
                     /translation="MDTVIAGLLGLMVGGVAGFVVRNTIGASNARSAEARAKKLLLDA
                     EEEAAKTRAQGLQESKDEAARMRRETEEDLKHRREEVARLERRITETESDLRERGQRM
                     DARTAELKEREDKLTFVREQLEKATDQHRVQLERIAGMTSAEAKDLLSSQVVDEAKRG
                     AMAQVREIEQRAREEGEERARKIVTIAIQRVASEQTAESSVSVFSLPSEDMKGRIIGR
                     EGRNIRAFEAATGVNIIIDDTPEAVVLSCFDPVRREAARMTLEKLVQDGRIQPARIEE
                     VHERSVRDLEEQIRKSGEDAVAEVGLTDVHPELIKLLGRLQYRTSYGQNVLKHLVESS
                     HIASALAAEIGIDPATAKRGAFLHDIGKAVTHEVEGSHAIIGAEIARRLKEDPEVVHC
                     IESHHGEVEQRTVHAVLAQIADSISGGRPGARRESLETYIKRLERLEEICTAHEGVEK
                     VFAMQAGREVRVMVTPGDVDDLQAQTIARDIAKQVEEELQYPGQIKISVIRETRATEF
                     AR"
     gene            1584..2921
                     /gene="miaB"
                     /locus_tag="VF195_13235"
     CDS             1584..2921
                     /gene="miaB"
                     /locus_tag="VF195_13235"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020541765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEX6581814.1"
                     /translation="MNGPFLIRTFGCQMNEHDSERIGGMLRADGMSPTDDVADARVIV
                     LNTCAIRENADNKLYGNLGHLKPLKTEHPDLRIVVAGCLAQKDQGVIQEKAPWVDVVV
                     GTHALPQLLDLLRRAEADGPQMDVAEYTDLFPSALPSARDDAFRAWVSIAVGCDNACT
                     FCIVPFVRGAQRSRAIGDVLAEVQGLVREGVVEVTLLGQNVNTYGRDVTVPGSSRRPL
                     FAELLRQVNEVEGLRRIRFTSPHPHDFTPDVIESMAESDKVCEHIHFPLQSGSDRVLK
                     AMQRSYRRDRYLGWLEAIRDAIPPIAVSTDIIVGFPGETEEDFTDTLDVVSRARFDSA
                     YTFQYSPRPATRAASMPEQVPKEVVQERFDRLLALQQEISLEKAGEQVGTEVEVLVEG
                     MGPRRSSTQARTRTNRIVHLAEPLEPGAFVIARITGAAAHHLTGEVVPAPARV"
     gene            2890..3858
                     /gene="miaA"
                     /locus_tag="VF195_13240"
     CDS             2890..3858
                     /gene="miaA"
                     /locus_tag="VF195_13240"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018785978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEX6581815.1"
                     /translation="MRSYRRPLACEAEGLTGVESFLALVGPTASGKTAAALRIAEALG
                     AEILSIDSMLVYRGMDVGTAKPTESERARIPHHLVDMVEPSQPFSVASFQVAARRAAE
                     DVRRRGETPLLVGGSGLYFRAVVDDLEFPGTEAGVREALEGEAAALGPDRMHRRLVEL
                     DPVAASRIEPGNVRRTVRALEVAAITGRTFSSFAAAWERYGPERVRAAGVRLGTQDLA
                     ARVDARVQEMLGAGWLKEVEALVARGLGGWLTASQAIGYAELARHLAGELSLEDAVQQ
                     TVKRTRTLARRQLAWFRRDPRIRWFDVGIGGALEATDEIASYLEGA"
     gene            3855..4715
                     /gene="dapF"
                     /locus_tag="VF195_13245"
     CDS             3855..4715
                     /gene="dapF"
                     /locus_tag="VF195_13245"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX6581816.1"
                     /translation="MSDVRVWKYQGTGNDFVMTLDLERERPLDRDDVRALCDRRFGIG
                     ADGSIRVTRGTEGLPFMDYRNADGSIAEMCGNGIRCVAVFLRDLGEETRPSFDVGTRA
                     GMRRVTFRSDDRVTVDMGEPNFTKAAIPMRGPAWETFLDESLDLGGGVVVQATALSMG
                     NPHLVLFLDEEPERFHVSHIGAVLERDDRFPEGTNVEFARVGADEIAARVWERGSGET
                     MACGSGACAIAVAAHEAGLADQRSRIRFPGGTIEVERQESGAVTLTGPAARVFETAID
                     IEALAEVAVR"
     gene            4712..5815
                     /gene="dapE"
                     /locus_tag="VF195_13250"
     CDS             4712..5815
                     /gene="dapE"
                     /locus_tag="VF195_13250"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEX6581817.1"
                     /translation="MTELLERIAAHTEALVAIPSESGNEAAMLDAVRAQLPGILTVVD
                     DEDAALLALPARRPGAPLILLAGHVDTVPLGGNAPARREGGTVHGRGAADMKGGIAVL
                     LEVAAALADGRLRSELDVGLLCFGREELPFAESALTPLFARRPEIRDTALAIVLEPTD
                     NALELGCLGNLNATVRFEGEAAHTARPWLGRNAIHAAVAALGPVNGSPIRDVDVGGLT
                     YREVASVTWIEGGIATNVVPAHAAARVNLRYAPARSPEDAERYLRELLAHPSATVTID
                     GNAPPGTVPVRNPLVDRLRAAGDLAVGPKQAWTPVAEFGLIGVDAVNFGPGDPQYAHR
                     DDERVEVDALVRSYDVLCAFLEGAPGREGRGEA"
     gene            5816..6985
                     /locus_tag="VF195_13255"
     CDS             5816..6985
                     /locus_tag="VF195_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX6581818.1"
                     /translation="MVHLSPGMRTSDPYPFEELDRRKAAALAAGRPIVDFGVGDPREE
                     TPAFIREALKDAIGPTSSYPRAAGLPELRGAIAAWVERRFGMRLDPDVHILPTLGSKE
                     PIFHLAQSLLDPAGGKHLVTVTTPGYTIPERGARFAGGDVARLPLTEPNGFLPDLDAV
                     DASTWDRTAILWLNYPNNPTGATAPLDFLRRAADHSRENDVLLASDEAYSELWFDGEP
                     PASVLQSGDLTNVLAFHTLSKRSSMTGYRSGFVAGDPELIAALKALRPSVGVTPQEFV
                     QRASVAAWSDEGHVEELRAVYARKRKLFLDLFASREVRVAASEATFYLWVVVPASTTS
                     LDWSIHLLEHADVILAPGPFFGPDGEGYVRMAMVPTIEECERAAEALDGVLGAGA"
     gene            6982..7803
                     /locus_tag="VF195_13260"
     CDS             6982..7803
                     /locus_tag="VF195_13260"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEX6581819.1"
                     /translation="MSTDHTELADRIERSWTEPDRLDAAAVEEAVGLLDRGELRVAEP
                     DGDAWKVNEWAKKAVLLYFRVRDLETTEVGPFEYHDRLPLKTGYGRAGVRVVPPATAR
                     YGAFLSPGVVLMPSYVNIGAWVGPGALVDTWATVGSCAQIGAGVHLSGGVGIGGVLEP
                     IQAGPVIVEDGAFVGSRCIVVEGVRVRSRAVLGAGLILTGNTPVIDVTGDESVEHRGE
                     VPAGAIVVPGSRSRDFPAGGYGLPAGLIIGYRTEATESKLQLNELLREFGSSVGS"
     gene            7807..9144
                     /gene="hflX"
                     /locus_tag="VF195_13265"
     CDS             7807..9144
                     /gene="hflX"
                     /locus_tag="VF195_13265"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX6581820.1"
                     /translation="MPERPKIRREADDGRQTRDRATLESPLSATWQPRVRERAVLVGV
                     GPGISEADLDELDALADSAGADAVSRIVQSRREPDPGTFIGRGKIEELHHTVHRLGAG
                     SVILDQELTPGQLRSLEERLGVKVIDRTALILDIFALHARSREGKAQVELAQLNYLLP
                     RLRGWGEAMSRLGGGIGTRGPGETKMEVDRQHIRRRISKLRRDIKTLTKTRDVKRSRR
                     QDSGIPQVAIAGYTNAGKSTLMNRLTGARTVVADQLFATLDPTTRRIELPGRRRAVIS
                     DTVGFVGKLPHDLVEAFRSTLEEVVLADLIVHVADATSSALDEQVDAVRRVLGEIGAG
                     QIPEVLALNKIDEVPGSARARLAGRFPGSVAVSALTGEGSDGLLEVIGARVPHPPVEV
                     TLLVPFGREDVTARLYREAEVLSKETDTVGTIVRVRVGLRLLSAIRPFVVDGS"
     gene            complement(9148..10137)
                     /locus_tag="VF195_13270"
     CDS             complement(9148..10137)
                     /locus_tag="VF195_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581821.1"
                     /translation="MQSSTKLESQIRSQPDELSHLLTSDRARDQTVVAADRLRRSKRI
                     WLVGTVTSHHAAILGAGMLQDAGRSAVAVSSMRFVVWAPIVGPDDAIILITHTGETAY
                     ALAARALATSAGLDVMMITKRGTPFPHAIETCPAETAETYTVSYTTALLVLAMIAKEM
                     GADSITEDTLALVPGAVRKAIEAAGTESVRVPERTLVIAGAGPAAFTAREGALKLREG
                     ARVMAEGHDIEYLLHGSAVPLDHRDAVLGLTTPDADGFVRAVMDAAAAEGIPVSTLGE
                     PAPLPMLLAQIPLTVRLQILALRLALERGQDPDTVITGNWADEELWRLGRPDR"
     gene            complement(10197..10805)
                     /gene="lexA"
                     /locus_tag="VF195_13275"
     CDS             complement(10197..10805)
                     /gene="lexA"
                     /locus_tag="VF195_13275"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013916633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEX6581822.1"
                     /translation="MSDMTSRQQRILDFIEKTVRERGYPPTVREIGEAVGLTSSSSVH
                     AQLANLERKGLLTKDPSKPRAMTLSDERRAEGTPVPLIGRIAAGEPVLAEEHVEDRIM
                     VPAGFVRGDGHFALRVIGESMVGAGILDGDVVVVRSQRSATDGDIVAALLPGPAEDEA
                     TVKRLGHDGARVMLIPENPALEPFEMLDGRILGKVVAVLRKL"
     gene            10968..11261
                     /locus_tag="VF195_13280"
     CDS             10968..11261
                     /locus_tag="VF195_13280"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEX6581823.1"
                     /translation="MGRTRVRRRRLVGLAIAIGFVGVLSGPVANAVGVGGGGEGGSAR
                     TYVVRPGDTLWSIARRSSPSVDPRLVVAAIASANRVDPGALVPGQELSIPTIT"
     gene            11344..11829
                     /gene="nrdR"
                     /locus_tag="VF195_13285"
     CDS             11344..11829
                     /gene="nrdR"
                     /locus_tag="VF195_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX6581824.1"
                     /translation="MRCPWCGADDDRVVDSRPTDGGSAIRRRRQCAVCARRYSTYERI
                     EDVGLVVRKRDDSRDPFRREKVTAGILKATKNRPVSEFQVEELVDRVEERLRRKGPEV
                     TSEQVGLEVLQQLRKLDEVAYIRFASVYKDFQEITDFERELGTLLKRVPAKRRRRKPK
                     A"
     gene            12151..14925
                     /locus_tag="VF195_13290"
     CDS             12151..14925
                     /locus_tag="VF195_13290"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010231590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX6581825.1"
                     /translation="MQVIEANEQDGLAFRRHFSTEGTHPFDEIEWETRDAVIPNFKEG
                     GNAFEQRNVEFPVSWSQNATNIVAQKYFRGHLGTPERESSVRQLVSRVVDTIRTWGEK
                     NHYFATEQDAEVFAHELTHLLVNQKAAFNSPVWFNVGVPDTPQQCSACFILAVEDHMS
                     SILNWYVEEGTIFKGGSGSGINLSRIRSSKEGLAGGGTASGPVSFMRGADASAGTIKS
                     GGKTRRAAKMVILNVDHPDVRDFIWCKAVEERKARVLRDAGFDMDLDGKDSHSTQYQN
                     ANNSVRVTDEFMRAFEQDQDWKLKAVLSGETVESTRARDLMRDIAQAAWECADPGMQY
                     DTTINEWHTCPASGRINGSNPCSEYMHLDNSACNLASLNLMKFVNEDGTFDVADFKHA
                     VEIVFTAQEILVGESSYPTEKIGINARAYRQLGLGYANLGALLMARGLAYDSDAGRAW
                     AGAITALMTGHAYRTSALVAEAMGPFEGYDPNADAMLRVMRKHREAADAIEGELVPEG
                     LLSAAKHSWDEAISLGERHGYRNAQASVLAPTGTIGLMMDCDTTGIEPELALVKTKKL
                     VGGGTMQFVNRTVPRALERLGYDAAAVEDIVAYIAEHNSVNDAPHLKPEHGSVFDTAM
                     GDAPIHYMGHVRMMGAAQPFISGAISKTVNMPEHVTVEEVEQLFVESWKLGLKAVAIY
                     RDNCKVAQPMSADKKKSAATTQSEGAGEPQTLPWSAKKRLPRSRPAITTSFRVGDAEG
                     YITAGSYPDDGLGEIFLKASKQGSTLSGIMDAFSIAVSVGLQYGVPLEDYASKFINMK
                     FEPSGLTDDEDIRFASSIVDFVFRRLAIDHLPPEKREALGIRSIEERKVEVAEKTAAP
                     AVTGGETTAVEEAPIEASEGAVIRPIEMVAKPTAKELDAPLCYQCGSKMQPAGSCYVC
                     GTCGSTSGCS"
     gene            14956..15336
                     /locus_tag="VF195_13295"
     CDS             14956..15336
                     /locus_tag="VF195_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018729619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581826.1"
                     /translation="MTHAEPGRLLGLHHVQLAMPPEEEQAAIDFYEGVLGLEQVAKPQ
                     ELADRGGVWFRSGNLEVHLGVEEGFQPAMKAHPAFLVEDLERMRARVEMSGYKVIESD
                     QFSGFRRVHVRDPFGNRLELIEPV"
BASE COUNT         2474 a         5162 c         5539 g         2272 t
ORIGIN      
        1 cggggaccga gatccctctc ccgagacatc gggaggaacg atggacaccg tcatcgccgg
       61 cctcctcggc ctcatggtcg ggggcgtcgc gggattcgtc gtccggaaca cgatcggagc
      121 gtccaacgcg cgctccgccg aagcacgagc gaagaagctc ctcctggatg ccgaagaaga
      181 ggccgccaag acgcgggccc aaggactcca ggaatccaag gacgaagccg cgcgtatgcg
      241 gcgggagacc gaggaggacc tcaagcatcg ccgcgaggag gtcgcgaggc tcgagcggcg
      301 catcaccgag accgaatccg atctccgcga gcgcggccaa cggatggacg cgcgtaccgc
      361 agagctcaag gaacgagagg acaagctcac gttcgtccgc gagcagctcg agaaggccac
      421 ggaccaacac agagtccagc tcgagcggat cgcgggcatg acgtcggcgg aagcgaagga
      481 cctcctctcg tcccaggtgg tcgacgaagc gaagcgcgga gcgatggcgc aggtgcgaga
      541 gatcgagcag cgcgctcggg aggagggcga ggaacgggct cggaagatcg tcacgatcgc
      601 gatccagcgg gtcgcgtcgg agcagacggc agagtcgagc gtcagcgtct tctcgctccc
      661 gagcgaggac atgaagggcc ggatcatcgg acgcgaaggt cgcaacatcc gggcgttcga
      721 ggccgccacc ggggtcaaca tcatcatcga cgacacgccg gaagctgtgg tcctgtcctg
      781 tttcgatccc gtgcgccgcg aggccgcgag gatgaccctg gaaaagctcg tccaggacgg
      841 caggatccaa cccgcgcgga tcgaggaggt ccacgagcgc tcggtgcgcg acctcgagga
      901 gcagatccgg aagtccgggg aggatgccgt cgcggaggtc ggtctcaccg acgtccatcc
      961 ggagctgatc aagctcctgg gccggctcca gtaccggacc agctacgggc agaacgtgct
     1021 gaagcacctc gtggagtcct cccacatcgc gagcgcactg gccgcggaga tcgggatcga
     1081 tcccgcgacc gccaagcgcg gcgcgttcct gcacgacatc ggcaaggccg tcacccacga
     1141 ggtcgaggga tcgcacgcga tcatcggcgc cgagatcgca cggcggctga aggaggaccc
     1201 cgaggtcgtc cactgcatcg agtcccatca cggggaggta gagcagcgga ccgtccacgc
     1261 cgtcctcgcg cagatcgcgg actcgatcag cgggggccgt cccggcgctc gccgggaatc
     1321 cctggagacc tacatcaagc ggctcgagcg gctcgaggag atctgcacgg cgcacgaggg
     1381 cgtcgagaag gtcttcgcga tgcaggccgg ccgcgaggtg cgcgtgatgg tcaccccggg
     1441 cgacgtggac gacctccagg cccagacgat cgcacgggac atcgcgaagc aggtcgagga
     1501 ggagctccag tacccgggac agatcaagat ctcggtgatc cgcgagacgc gcgcgaccga
     1561 gttcgcacgg tagcctcgat ggcgtgaacg ggccgttcct gatccgcacc ttcgggtgcc
     1621 agatgaacga gcacgactcc gaacggatcg gaggcatgct ccgcgcggac gggatgtcgc
     1681 ccaccgacga cgtcgccgac gcgagggtta tcgtcctgaa cacgtgcgcg atccgtgaga
     1741 acgccgacaa caagctctac ggcaaccttg gtcatctgaa gcctctcaag accgagcacc
     1801 cggacctccg catcgtggtc gccggctgcc tcgcccagaa ggaccagggc gtgatccagg
     1861 agaaggctcc ctgggtcgac gtcgtggtcg gaacgcacgc gctcccgcag ctgctcgacc
     1921 tcctccggcg ggccgaggcg gacggacccc agatggatgt cgccgagtac accgacctgt
     1981 tcccgtcggc gctgccgtcg gcgcgtgacg acgccttccg cgcctgggtc tcgatcgcgg
     2041 tcgggtgcga caacgcctgc acgttctgca tcgtgccgtt cgtccgcgga gcgcagcgct
     2101 cgcgagcgat cggcgacgtg ctcgccgagg tccaggggct cgtacgggag ggcgtggtcg
     2161 aggtgacgtt gctcggtcag aacgtgaaca cgtacgggcg ggacgtgacg gtccccgggt
     2221 cgtcgcggag acccttgttc gccgagctgc tccggcaggt gaacgaggtc gaggggctcc
     2281 gccggatccg gttcacgtcg ccgcatccgc atgacttcac gcccgacgtg atcgagtcga
     2341 tggcggagag cgacaaggtc tgcgagcaca tccatttccc gctgcaatcg gggtcggacc
     2401 gcgtcctgaa ggcgatgcag cgctcgtacc gacgagatcg gtacctggga tggctcgagg
     2461 cgatccgcga cgcgatcccc ccgatcgcgg tgtcgacgga catcatcgtc ggcttccccg
     2521 gcgagacgga ggaggacttc accgatacgt tggacgtcgt gtcgcgcgcg cggttcgact
     2581 ccgcgtacac cttccagtac tcgccgcgac ccgccacccg cgccgcctcg atgccggagc
     2641 aggtgccgaa ggaggtcgtc caggagcggt tcgatcggtt gctggcgctc cagcaagaga
     2701 tctcgctcga gaaggcgggc gagcaggtcg gcaccgaggt ggaggtgctc gtggagggaa
     2761 tgggcccgcg acgatcctcg acgcaggcac ggacccggac gaacaggatc gtccatctgg
     2821 cggagccgct cgagccgggc gcgttcgtga tcgcgcgcat cacgggtgcc gcggcgcatc
     2881 acctcaccgg tgaggtcgta ccggcgcccg ctcgcgtgtg aggccgaggg gctgacgggg
     2941 gtggagtcct tcctcgcatt ggtcggtccg accgcatccg ggaagaccgc tgccgctctc
     3001 cggatcgccg aggccctggg cgcggagatc ctctcgatcg actcgatgct cgtgtaccgc
     3061 gggatggatg tcggaaccgc caagcccacg gagtcggagc gtgctcggat cccccatcac
     3121 ctcgtggaca tggtcgaacc gagtcagccg ttcagcgtgg cctcgttcca ggtcgcggcc
     3181 cgccgggccg cggaggacgt gcgccgtcgc ggggagaccc cgctgctcgt gggaggctcg
     3241 gggctgtact tccgggcggt cgtggacgac ctggagttcc cgggaaccga ggcgggggtc
     3301 cgggaggcct tggagggcga ggcggcggcg ctcggccccg atcggatgca ccggcgcctc
     3361 gtcgagctgg atccggtggc ggcgtcgagg atcgagccgg ggaacgtccg gcggaccgtc
     3421 cgcgccctcg aggtggccgc gatcacgggg aggacgttca gctccttcgc ggcggcctgg
     3481 gaacggtacg ggccggaacg cgtccgcgcc gcgggcgtcc gtctcgggac ccaggatctc
     3541 gcggcgaggg tggacgcacg tgtccaggag atgctgggag cagggtggct gaaggaggtc
     3601 gaggccctcg tggctcgggg gctcggcggc tggctcaccg cctcccaggc catcggctac
     3661 gctgagctcg cccgccatct cgcaggagag ctcagcctgg aggacgccgt ccagcagacc
     3721 gtcaagcgca cccgaaccct cgcgcggcgg cagttggcgt ggttccgtcg cgaccctcgc
     3781 atccggtggt tcgacgtcgg gatcggcgga gccctcgagg cgaccgatga gatcgcctcc
     3841 tacctggagg gcgcgtgagc gacgtccgcg tctggaagta ccaggggacc ggcaacgact
     3901 tcgtgatgac gctcgacctc gagcgagagc gacctctgga tcgcgacgac gtccgggcgc
     3961 tctgcgaccg ccggttcggg atcggcgcgg acggctcgat ccgcgtcacg cgggggaccg
     4021 agggccttcc tttcatggac tatcggaacg cggacggcag catcgccgag atgtgcggca
     4081 acggcatccg ctgcgtcgcc gtgttcctcc gggacctcgg ggaggagacg cggccgtcgt
     4141 tcgacgtcgg cacgcgcgca ggcatgcggc gggtgacgtt ccggtcggac gatcgcgtca
     4201 ccgtcgacat gggagagccg aacttcacga aggcggccat cccgatgcga gggccggcat
     4261 gggagacgtt cctcgacgaa tcgctcgacc tcgggggcgg cgtcgtcgtg caggcgaccg
     4321 cgctgtcgat ggggaaccct cacctcgtgc tcttcctcga cgaggagccc gagcggttcc
     4381 acgtgtccca catcggcgcg gttctggaac gggacgaccg attcccggag gggacgaacg
     4441 tggagttcgc ccgcgtcggc gcggacgaga tcgcggcgag ggtctgggag cgcgggagcg
     4501 gcgagacgat ggcgtgcggg agcggggcgt gcgcgatcgc cgtcgccgcg cacgaggccg
     4561 gtctcgccga tcagcggtcg aggatccggt tcccgggcgg gacgatcgag gtcgaacgcc
     4621 aggagtcggg cgccgtcacc ctgaccggac cggcggcgcg ggtgttcgag acggcgatcg
     4681 acatcgaggc gctcgccgag gtcgccgtcc ggtgacggag ctcttggaac gcatcgccgc
     4741 ccacacggag gcgctcgtgg ccatcccctc ggaaagcggg aacgaggccg cgatgctgga
     4801 tgcggtccgc gcgcagctcc cgggcatcct cacggtcgtc gacgacgagg acgcggcgct
     4861 cctcgccctc ccggcgcggc gcccgggggc gcccctgatc ctgctcgccg gtcacgtgga
     4921 cacggtgccc ctcggcggaa acgcccccgc gcgccgagaa ggtggcacgg tccacggccg
     4981 gggagcggcg gacatgaagg gcgggatcgc cgtgctcctg gaggtggccg cggcgctcgc
     5041 ggacggccgt ctccgatccg agctcgacgt ggggctcctg tgcttcggcc gggaggagct
     5101 cccgttcgcg gagagcgcgc tcaccccgct gttcgcgcgc cgcccggaga tccgcgacac
     5161 cgccctcgcg atcgtcctcg aacccaccga caacgccctc gagctgggct gcctcgggaa
     5221 cctgaacgcc accgtgcggt tcgagggcga ggcggcgcac acggctcggc cctggctcgg
     5281 acgcaacgcg atccacgccg cggtcgccgc gctcgggccc gtcaacggat cgccgatcag
     5341 ggacgtggac gtgggcgggc tcacgtaccg ggaggtcgcc tccgtcacgt ggatcgaggg
     5401 cggcatcgcg acgaacgtgg tgcctgcgca tgcggctgcg cgggtgaacc tccggtacgc
     5461 gcccgcgcgg tcgcccgagg acgcggagcg gtacctgcga gagctcctcg cgcatccgtc
     5521 cgcgaccgtg acgatcgacg gcaacgcgcc gccaggcacg gtcccggtga ggaacccgct
     5581 ggtcgaccgg ctccgggccg ccggcgacct cgccgtcggc ccgaaacagg cgtggacccc
     5641 cgtggccgag ttcggtctga tcggggtgga tgcggtgaac ttcggaccgg gcgacccgca
     5701 gtacgctcac agggacgacg agcgcgtcga ggtagacgcg ctcgtccggt cctacgacgt
     5761 gctgtgcgcg ttcctggagg gcgcgcccgg gagggaaggt cgcggagagg cgtagatggt
     5821 gcacctgtca ccggggatgc gaacctcgga tccgtacccg ttcgaggagc tggaccgtcg
     5881 gaaggccgcc gcgctggcgg ccggacgtcc gatcgtcgac ttcggtgtgg gcgatccgcg
     5941 ggaggagacg ccggcgttca tccgcgaggc cctgaaggac gcgatcgggc cgacctcctc
     6001 gtatccgcgc gccgccggtc tcccggagct ccgcggcgcg atcgccgcct gggtcgagcg
     6061 tcggttcggc atgcgcctcg atcccgacgt ccacatcctg ccgaccctgg gttcgaagga
     6121 gccgatcttc catctcgcgc agtcgctcct cgaccccgcc ggagggaagc atctcgtgac
     6181 cgtgacgacc ccgggctaca cgatccccga gcggggcgcg cggttcgccg gcggtgacgt
     6241 cgcccgcctg cccctcaccg agccgaacgg gttcctgccc gatctggacg cggtcgacgc
     6301 ctccacctgg gaccgcaccg cgatcctgtg gctgaactat ccgaacaacc cgacgggcgc
     6361 gacggcgccg ctggacttcc tccggcgagc cgccgaccac agccgggaga acgacgtgct
     6421 cctcgcctcc gacgaggcgt acagcgagct ctggttcgac ggcgagccgc cggcctcggt
     6481 cctccagtcg ggggacctga cgaacgtcct cgcgttccac acgctgagca aacgctcgtc
     6541 gatgacgggc taccggtcgg ggttcgtcgc cggcgatccc gagctgatcg cagcgctgaa
     6601 ggcgctccgg ccgtcggtcg gggtgacgcc gcaggaattc gtgcagcgcg cgtcggtcgc
     6661 ggcgtggagc gacgagggcc acgtcgagga gctgcgcgcg gtctacgccc ggaagcggaa
     6721 gctgttcctc gatctcttcg cgtcgcgcga ggtacgggtc gcggcgagcg aggccacgtt
     6781 ctacctctgg gtggtcgtcc cggcatccac cacctcgctc gactggtcga tccacctgct
     6841 tgagcacgcg gacgtgatcc tcgctcccgg tcccttcttc ggaccggacg gggaggggta
     6901 cgtccggatg gcgatggtgc cgacgatcga ggagtgcgag cgtgccgcgg aggcgctcga
     6961 cggcgtgctg ggagcaggcg cttgagcacg gatcacacgg aactcgcgga caggatcgag
     7021 cggtcctgga ccgagcccga tcggctcgat gccgcggccg tggaagaggc ggtcgggctg
     7081 ctggaccgtg gggagctccg cgtagccgag cccgacggcg acgcctggaa ggtgaacgag
     7141 tgggcgaaga aggcggtgct cctctacttc cgggtgcgtg acctcgagac gaccgaggtc
     7201 ggcccgttcg agtatcacga ccggcttccc ctgaagaccg gctacggacg ggcgggggtg
     7261 cgcgtcgtcc ctccggcgac ggcccgatac ggcgcgttcc tgtcacccgg tgtcgtcttg
     7321 atgccgagct acgtgaacat cggcgcctgg gtcggccccg gagcgctcgt cgacacctgg
     7381 gccacggtcg ggtcctgcgc gcagatcggc gcgggggtgc acctctcggg cggcgtcggg
     7441 atcggcggcg tgctggagcc gatccaggcc ggcccggtga tcgtggagga cggtgcgttc
     7501 gtgggctcgc gttgcatcgt ggtcgagggc gtccgggtgc gctcgcgcgc ggtgctggga
     7561 gccgggctga tcctcaccgg caacacgccg gtgatcgacg tcaccggcga cgaatccgtg
     7621 gagcaccggg gagaggtgcc ggccggggcg atcgtcgtgc ccggctcgcg ctcgcgcgac
     7681 ttccccgccg gcgggtacgg ccttccggcc ggactgatca tcggctaccg gaccgaggcg
     7741 acggaatcca agctccaact caacgagctt ctccgcgagt tcggctcgtc ggtgggctcc
     7801 tagcggatgc cggagcggcc gaagatccga cgcgaggcgg atgacggccg ccagacacgg
     7861 gaccgcgcca ccctcgagtc gccgctgtcc gcgacgtggc agccacgcgt ccgtgaacgg
     7921 gccgtgcttg tcggtgtcgg acccggcatc agcgaggcgg acctcgacga gctcgacgcg
     7981 ctggcggact ccgccggcgc cgacgccgtc tcccggatcg tgcagagtcg ccgggagccc
     8041 gatcccggca cgttcatcgg ccggggcaag atcgaggagc tccaccacac ggtgcatcgc
     8101 ctcggcgcgg gatcggtgat cctggaccag gagctcacgc cggggcagct ccggagcctc
     8161 gaggagcgac tcggcgtgaa ggtgatcgat cgcacggcgt tgatcctcga catcttcgcc
     8221 ctgcacgcga ggagccggga gggcaaggca caggttgagc tcgcccagct gaactaccta
     8281 ctgccacggc tccgtggctg gggtgaggcg atgagccgcc tggggggagg gatcggcacg
     8341 cggggtccgg gcgagacgaa gatggaggtc gaccgtcagc acatccgccg ccgcatctcg
     8401 aagctccgcc gtgacatcaa gacgctcacg aagacccggg acgtcaagcg ttcccgccgt
     8461 caggattccg ggatccctca ggtggcgatc gccggctaca ccaacgcggg caagtcgacc
     8521 ctgatgaacc gcctgaccgg ggcgaggacg gtcgtggccg atcagctctt cgcgacgctc
     8581 gatcccacca cccgtcggat cgagctcccc ggccggcgtc gcgccgtgat ctccgacacc
     8641 gtcgggttcg tgggcaagct gccccacgac ctggtggagg ccttccgctc caccctcgag
     8701 gaagtggtcc tggccgacct gatcgtgcac gtcgccgacg ccacgtcgtc ggcgctcgac
     8761 gaacaggtgg acgccgtccg gcgggtcctc ggtgagatcg gggccggcca gatccctgag
     8821 gtcctcgcgt tgaacaagat cgacgaggtc cccgggtccg ctcgggcgag gctcgccggc
     8881 cggttcccag gctcggtcgc ggtctccgcg ctcaccggcg agggctcgga cggtctgctc
     8941 gaggtgatcg gggcgcgcgt gccccatccg cctgtcgagg tgacgctgct cgtccccttc
     9001 ggacgggagg acgtgacggc gcgcctctac cgggaggccg aggtgctgtc gaaggagacc
     9061 gacacggtcg ggacgatcgt gcgggtccgc gtgggtttgc ggctgctgtc cgcgatccgt
     9121 ccgttcgtcg tggacggaag ctgagcccta ccgatccggc cgacccagcc tccagagttc
     9181 ctcatccgcc cagttgccgg tgatcaccgt gtcgggatcc tgaccgcgct cgagcgcgag
     9241 ccggagcgcg aggatctgca gccggaccgt gagcgggatc tgggccagga gcatcgggag
     9301 gggcgccggc tcgcccagcg tggagaccgg gatgccctcg gcggcggcgg cgtccatcac
     9361 ggccctcacg aagccgtccg cgtcgggcgt cgtcaggccg agcacggcgt cccgatgatc
     9421 gaggggcaca gccgagccgt ggaggaggta ttcgatgtcg tgcccttctg ccatcaccct
     9481 ggcgccctca cgcagcttca gcgcgccttc gcgtgcggtg aacgcggcgg gcccggcgcc
     9541 cgcgatcacg agcgtccgct cggggacccg gacggactcg gtcccggccg cctcgatcgc
     9601 cttgcgcacc gctccgggaa cgagcgcgag cgtgtcctcc gtgatcgagt ccgcgcccat
     9661 ctccttcgcg atcatcgcca gcacgagcag cgcggtcgtg tagctcaccg tgtaggtctc
     9721 cgcggtctcg gccgggcacg tctcgatcgc gtgcgggaag ggtgtgccgc gcttcgtgat
     9781 catcatcacg tccaggccgg cgctcgtcgc gagtgcgcgg gcggcgagcg cataggccgt
     9841 ctcgcccgtg tgggtgatca ggatgatcgc gtcgtcgggg ccgacgatgg gggcccagac
     9901 gacgaagcgc atcgacgaaa cggcgacggc cgaacggccc gcgtcctgga gcatcccggc
     9961 tccgaggatc gcggcgtgat ggctcgtgac ggtcccgacg agccagatcc gcttcgaccg
    10021 tctgaggcga tcggccgcca caacagtctg gtcccgggca cgatcggagg tcaggaggtg
    10081 ggagagctcg tccggctgcg aacggatctg gctctccagc ttggtgctcg actgcatgga
    10141 catcggtcgg gttcccctgg cctctcggcg tcgtcggggg tggagcttag cccgactcag
    10201 agcttcctga gcaccgccac gaccttgccg aggatcctgc cgtcgagcat ctcgaaaggc
    10261 tcgagcgccg ggttctcggg gatgagcatg acgcgggcgc cgtcgtgacc gagacgcttg
    10321 acggtggcct cgtcctccgc cggtccgggc aggagcgcgg cgacgatgtc gccatcggtc
    10381 gccgatcgct ggctccgcac gaccacgacg tcgccgtcga ggatgcccgc gccgaccatc
    10441 gactcgccga tgacccgcag cgcgaaatga ccgtcgcccc gcacgaatcc ggccggcacc
    10501 atgatgcggt cctcgacgtg ctcctccgcg aggacgggct cacctgccgc gatccggccg
    10561 atcagaggga ccggcgtccc ctccgcccgt cgctcgtccg agagcgtcat ggctcggggc
    10621 ttgctcgggt ccttcgtgag gaggcccttc cgttcgaggt tggccagttg ggcgtggacg
    10681 gatgatgagg aggtgaggcc tacggcttcg ccgatctcac ggaccgtcgg cgggtagcca
    10741 cgctcgcgca cggtcttctc gatgaaatcg aggatgcgct gctgccgcga agtcatgtca
    10801 gacatggtgc gtagcctcct gttcccggcg gcgcctctcg gggccatctt ggggccagag
    10861 gtcggcgccg ctgccgaagg aggacgctag cacccgctcg gaggagacgc aaacatatgt
    10921 tcggttctcg ccttgacatc gaacacgcgt tcggccagga tgtgcacatg ggtcgaacac
    10981 gcgttcgacg gaggcgcctg gtcgggctgg ccatcgccat cgggttcgtc ggggtcctct
    11041 ccggaccggt ggccaacgcc gtcggtgtcg gcggtggcgg ggaaggaggc tccgctcgca
    11101 cgtacgtcgt gcgtcccggg gacaccctgt ggtccatcgc ccggcggtcg tctccgtcgg
    11161 tggatccgcg cctcgtggtg gccgccatcg cgtctgccaa ccgggtggat cccggtgccc
    11221 tggtcccggg acaggagctg tccatcccca ccatcacctg accgcagggg tcgatgggtt
    11281 tgaccacacc acatcctgcg ggtaccgtgc ccgcggccac cacatcttgt ggccgcaacc
    11341 gacatgagat gcccatggtg cggcgcggac gacgatcggg tcgtcgactc acggcccacg
    11401 gacggtggat cggcgatccg ccgtcgccgg cagtgcgcgg tctgcgcccg ccggtattcc
    11461 acgtacgagc ggatcgagga cgtcgggctc gtcgttcgca agcgggacga ctcgagggat
    11521 ccgttccgtc gggagaaggt gaccgcgggc atcctcaagg cgacgaagaa ccggccggtc
    11581 tcggagttcc aggtcgagga gctcgtagac cgcgtggagg aacggctccg tcgcaagggg
    11641 cccgaggtca cctccgagca ggtcggtctc gaggtcctgc agcagctccg caagctcgac
    11701 gaggtcgcct acatccgatt cgcaagcgtc tacaaggact tccaggagat caccgacttc
    11761 gagcgcgagc tcgggaccct gctcaagcgg gttcccgcca agcgccggcg acgcaagccg
    11821 aaggcttgac gtcgggcgaa caaggtcgcc aaggatcgcg ttcccaccgg tcgtccactg
    11881 gcgttccaca ccctgcctcg gctagcttcg gggctgggtt cggatcttct gctgaaacca
    11941 caacatgtag cggtcggagg tgggagtaac accccacatc tagcaaacta gagacttgac
    12001 acgagggccg ctcgggggtt ggatgacatc cttgtgattc cacggatgtg atccatccgc
    12061 cgggactcct gaccctcagg actcccaaca ggttcttccc aggagaggag ggcgacgcag
    12121 gtggcgaaga ccagcgacga cctgcaggac gtgcaggtga tcgaggccaa tgaacaggat
    12181 ggcttggcgt tccgacggca cttctcgacc gagggaacgc atcccttcga tgagatcgaa
    12241 tgggagacac gcgacgccgt catcccgaac ttcaaggagg gcggcaacgc cttcgagcag
    12301 cggaacgtcg agttccccgt gtcctggtca cagaacgcca ccaacatcgt cgcccagaag
    12361 tacttccgcg gacacctcgg gacgcccgag cgtgagtcct cggtccggca gctcgtgtcc
    12421 cgggtcgtcg acacgatccg cacctggggg gagaagaacc actacttcgc taccgagcaa
    12481 gatgccgagg tcttcgcgca cgagctgacc cacctgctcg tgaaccagaa ggccgccttc
    12541 aattcgccgg tgtggttcaa cgtcggtgtt cccgacacgc cacagcagtg ctcggcgtgc
    12601 ttcatcctcg ccgtcgagga ccacatgtcg tcgatcctca actggtacgt cgaggagggg
    12661 acgatcttca agggcggatc cggatccggg atcaacctgt cgcggatccg ttcctcgaaa
    12721 gaggggctcg ccgggggagg gaccgcatcc ggacccgtga gcttcatgcg cggcgccgac
    12781 gcctccgccg gcacgatcaa gtcgggcggg aagacgcggc gcgcggccaa gatggtgatc
    12841 ctcaacgtcg atcaccccga cgtgcgggac ttcatctggt gcaaggcggt cgaggagcgc
    12901 aaggccaggg tcctacgcga cgccgggttc gacatggacc tcgacggcaa ggacagccac
    12961 tcgacccagt accagaacgc gaacaactcg gtccgtgtca cggacgagtt catgcgcgcc
    13021 ttcgagcagg accaggactg gaagctcaag gccgtcctgt cgggcgagac ggtcgagagc
    13081 acccgggccc gcgacctgat gcgggacatc gctcaggccg cgtgggagtg cgccgatccg
    13141 ggcatgcagt acgacacgac gatcaacgag tggcacacct gcccggcgtc ggggcggatc
    13201 aacggctcca acccctgcag cgagtacatg cacctcgaca actccgcatg caacctcgcg
    13261 tcgctgaacc tgatgaagtt cgtgaacgag gacggcacct tcgacgtcgc ggacttcaag
    13321 cacgccgtgg agatcgtctt cacggcgcag gagatcctgg tgggcgagtc gagctacccc
    13381 acggagaaga tcgggatcaa cgcgcgggcc taccgccagc tcggtctggg ctatgccaac
    13441 ctgggtgccc tcctgatggc gcgcggcctg gcgtacgact ccgatgccgg cagggcctgg
    13501 gccggtgcca tcacggcgct gatgaccgga catgcctacc ggacctcggc gctcgtggcc
    13561 gaggcgatgg gtcctttcga gggatacgac ccgaacgcgg acgcgatgct ccgcgtgatg
    13621 cgcaagcatc gggaggccgc ggacgcgatc gagggtgagc tcgtccccga agggctgctc
    13681 tcggctgcga agcactcgtg ggacgaggcc atctcgctcg gcgagcggca cgggtatcgc
    13741 aacgcgcagg cctccgtcct cgcgcccacc ggcacgatcg ggctgatgat ggactgcgac
    13801 acgacgggca tcgagcccga gctcgcgctc gtcaagacga agaagctcgt cggcggaggc
    13861 acgatgcagt tcgtgaaccg caccgtgccg cgggcgctcg agcggctcgg ctacgacgcc
    13921 gccgcggtcg aggatatcgt cgcctacatc gcggagcaca actcggtgaa cgacgcgccg
    13981 cacctcaagc cggagcacgg ctccgtcttc gacaccgcga tgggcgacgc cccgatccac
    14041 tacatggggc atgtccggat gatgggggcg gcgcagccgt tcatctcggg cgcgatctct
    14101 aagaccgtga acatgcccga gcacgtgacc gtggaggagg tcgagcagct cttcgtggag
    14161 agctggaagc tcggcctgaa ggcggtcgcg atctaccgcg acaactgcaa ggtggcgcag
    14221 cccatgtcgg ccgacaagaa gaagtcggct gcaacgacac agagcgaggg ggcgggcgag
    14281 ccgcagaccc ttccgtggtc cgcgaagaag cgtctcccgc gctcgcggcc cgccatcacc
    14341 acctcgttcc gggtcggtga cgccgagggc tacatcacgg cggggtccta cccggacgac
    14401 ggcctcggcg agatcttcct gaaggcctcg aagcaggggt ccacgctctc cgggatcatg
    14461 gacgcgttct cgatcgcggt ctcggtcggt ctgcagtacg gcgttccgct cgaggactac
    14521 gcctcgaagt tcatcaacat gaagttcgag ccgagcggtc tgacggacga cgaggacatc
    14581 cgattcgcat cctcgatcgt cgacttcgtg ttccgccggc tggcgatcga ccacctgcca
    14641 cctgagaagc gggaagcgct cgggatccgg tcgatcgagg agcggaaggt cgaggtcgcg
    14701 gagaagaccg ctgccccggc agtcaccggg ggagagacca cggccgtgga ggaagcgccg
    14761 atcgaggcgt cggagggagc ggtgatccgt cccatcgaga tggtcgccaa gcccacggcg
    14821 aaggagctgg acgcacccct gtgctaccag tgcggctcca agatgcagcc ggccggttcg
    14881 tgctacgtct gcgggacgtg cggcagcacc agcggctgct cgtaggatgg gcccctcgca
    14941 gggggacgag gatcgatgac ccacgctgag cccggcaggc tgctcgggct gcaccacgtg
    15001 cagctcgcga tgccaccaga agaggagcag gcggccatcg acttctacga gggtgtcctc
    15061 gggctcgagc aggtggcgaa gcctcaggag ctcgccgacc gcggcggcgt ctggttccgc
    15121 tcgggaaacc tcgaggtcca cctcggcgtc gaagaggggt tccagcccgc gatgaaggcg
    15181 caccccgcgt tcctggtcga ggacctcgag cggatgcgcg cccgtgtgga gatgagcggc
    15241 tacaaggtga tcgagtccga tcagttctcc ggcttccggc gggtgcacgt gcgcgacccg
    15301 ttcggcaacc gcctcgagct gatcgagccg gtctgacgat ggatctcccg ttccgacgcg
    15361 tgcgacccga ggggaagctg tcgagcgcgc agcatcccgg cgacgcgggc ctcgacctcc
    15421 gggcggccga gggcgtcacg gtcaagc
//
LOCUS       DASUYD010000254         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_863427, whole genome shotgun sequence.
ACCESSION   DASUYD010000254 DASUYD010000000
VERSION     DASUYD010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863427"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF195_13300"
     CDS             complement(<1..327)
                     /locus_tag="VF195_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018637277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HEX6581827.1"
                     /translation="MDTAQRIVHGVDRAHQRRPWIAFPYAVVKKFGDDQAGNLAALVA
                     YYGFFSLFPLMLVLVTVLGIVLQGNIELQERIESSALANFPVIGDEISSNVHPLRGSG
                     LALGIGI"
     gene            461..940
                     /locus_tag="VF195_13305"
     CDS             461..940
                     /locus_tag="VF195_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005266428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581828.1"
                     /translation="MDILGLVVGALLGTLVMTAVMETAQAARITRISIPFMLGAMVSD
                     RRATIRVAGFAMHLANGLIFALGYALLFEVLGRSDWWIGAAAGALHGALALVILLPVL
                     QDVHPRMAAEDQGPDPTPMLQPPGFLALNYGKRTPLVTVAAHVLYGTIVAALYPPLG"
     gene            complement(922..2724)
                     /locus_tag="VF195_13310"
     CDS             complement(922..2724)
                     /locus_tag="VF195_13310"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX6581829.1"
                     /translation="MDISTDVPIERYGLIGDCGTAALVSDEGSIDWLCLPSFDADPVF
                     GRLLDPGGGHCTMRAAELVGTQRRYLPGTPVLETTFRTATGIATLTDLFASVPAVRRE
                     TELWPFRWLIRRVEAEEGWVALDAEVSPRDPFGGSRWDLRSHGRSLAATSGGRGILAA
                     CTTPFSVAQDTAAARIEIGSGDVAYLCLAYADRDIGIMPPIGAAAERAFKDTVACWRE
                     WSNRIQGAPEQRAAVERSAITLKLLTFAPSGGVVAAPTTSLPEAIGSERNWDYRHVWI
                     RDASRSVIALLDRGYPEEAKAYLFWIANAARLTRPSLDTLYALNGERARRETEVAGLR
                     GYAGSLPVRDGNAAAGQLQLDNWGYVVDAAFAFADRTGELPWDLWASVRAFVNFAAAN
                     WRRADHGIWESRDRPRHFVHSKVMCWVALDRGLRLAGLLREDAPVVEWERERAEIHAA
                     VLIHGVDADRGTFMRAFDDPAVDAALLELPIVGFLPGDDERVVATVEQVRTELGRDEL
                     VMRYRTDDGLSGDEGGFLPCSFWLAHALACAGVRDQAADVFERACGRANDVGLLPEEI
                     DPVTGGFLGNFPQGLSHLALLAAAGALEDQPSGG"
     gene            3038..3181
                     /locus_tag="VF195_13315"
     CDS             3038..3181
                     /locus_tag="VF195_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581830.1"
                     /translation="MIDSRAEYVDAEGESSQVASCDIYDFIDGSLAAITTYAVEIDSS
                     AAD"
     gene            3220..4227
                     /locus_tag="VF195_13320"
     CDS             3220..4227
                     /locus_tag="VF195_13320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX6581831.1"
                     /translation="MKAIVQERYGSPDVLELREIERPNVEDDEVLVRVSAASVHADVW
                     HAVHGVPYVLRIMGSGLRAPKHPVPGTDLAGHVESVGRNVTRFRPGDEVFGQTLTANL
                     WRNGGAYAEYAAAPEATLELKPDLLTFEQAGAVPTSGSLAVQAVRDEGRTRSGQSVLI
                     NGAGGAVGTFAVQLAKAYGAEVTGVDAPGKLDVLRSIGADRVVDYTREDFTQSGERYD
                     LIIDIAGTRPWSASRRALGSEGTYVLIGHDQYGGSGHRWFGSLGRFFKLMVVSPFVSQ
                     LHPFRGAKDPGDRLIVMKELIEAGKITPVIDRTFPLSEVPEAIRYLGSGQAGGKIVIT
                     V"
     gene            complement(4251..>4449)
                     /locus_tag="VF195_13325"
     CDS             complement(4251..>4449)
                     /locus_tag="VF195_13325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HEX6581832.1"
                     /translation="RLKEWRRKRANADGVPAYVVFHDRTLAEIAERRCKDWADLAAIS
                     GVGPAKLERYADEVLAVVAAG"
BASE COUNT          754 a         1514 c         1474 g          707 t
ORIGIN      
        1 gatcccgatc ccgagcgcga gcccgctccc tcgcagcggg tggacgttgc tcgagatctc
       61 gtccccgatg accgggaagt tcgcgagcgc ggagctctcg atcctctctt ggagttcgat
      121 gttgccctgg agcacgatcc ccaggacggt gaccaagacg agcatcaggg ggaacaagga
      181 gaagaacccg tagtacgcca ccagcgcggc gaggttgccc gcctggtcat caccgaactt
      241 cttcacgacc gcgtagggga acgcgatcca cggccggcgc tgatgcgctc ggtccacacc
      301 atgcacgatc cgctgagcgg tatccatgac gcctcctcgt gaccaaggcg catcccaacg
      361 gcttgtgttt cacccgtgca tgcgattcca aaccttacga tcaaggagca cgtcagacgc
      421 agcaactgct tgtctgcaca cggcttgcta gcgtgcagcc atggacatcc tcggcctggt
      481 cgtcggggcc ctgctcggca cgctcgtgat gaccgccgtg atggaaaccg cgcaggcggc
      541 ccggatcacg cgcatcagca tcccgttcat gctcggagcg atggtgtcgg atcggcgagc
      601 gaccatccgg gtggcggggt tcgcgatgca tctcgccaac gggctgatct tcgcactcgg
      661 ctacgccttg ctcttcgagg ttctgggccg atcggattgg tggatcggtg cggccgccgg
      721 cgcgcttcac ggcgcactcg cgctcgtcat ccttctaccg gtgcttcagg acgttcatcc
      781 ccggatggct gcggaagacc aaggacccga cccgacgccg atgcttcagc cgccgggatt
      841 cctcgctctc aactacggca agcggacccc gctcgtgacg gtcgctgcgc acgtgctcta
      901 cggaaccatc gttgccgccc tctacccccc gctcggctga tcctccagcg cgccggcggc
      961 ggccaagagc gcgaggtggg acaacccctg tgggaagttc ccgaggaagc cgccggtgac
     1021 gggatcgatc tcctctggca agagaccgac gtcgttggcg cggccgcacg cgcgctcgaa
     1081 gacgtccgcg gcttgatcgc gcaccccagc gcacgcgagc gcgtgagcca gccagaacga
     1141 gcaaggaagg aagccacctt cgtcgccgga caagccgtcg tcggtgcggt accgcatcac
     1201 cagctcgtcg cggccgagct cggtccgcac ctgctccacg gtggcgacga cgcgctcatc
     1261 gtcccccggc aagaacccca cgatggggag ctcgagcaat gctgcgtcga ccgccgggtc
     1321 gtcgaaagcg cgcatgaagg tgcctcgatc agcgtcgacc ccatggatga gcaccgccgc
     1381 gtggatctcc gcccgttccc gttcccattc gacgaccggc gcgtcctcgc gaagcagccc
     1441 cgccaggcgg agcccgcggt cgagtgctac ccagcacatg accttcgagt gcacgaaatg
     1501 ccgcggccgg tcgcgggatt cccagatgcc gtggtctgct cggcgccagt tcgcggcggc
     1561 gaagttgacg aaggcgcgca ccgacgccca caggtcccac ggaagctcgc ccgtgcggtc
     1621 ggcgaacgcg aaggccgcgt cgacgacgta gccccagttg tcgagctgaa gctgcccggc
     1681 tgcggcgttc ccgtcgcgga ccggaagcga gcccgcgtag cccctgaggc cggcgacctc
     1741 cgtttcgcgc ctcgcgcgtt cgccgttcag cgcgtagagg gtgtcgaggc tgggtctggt
     1801 cagcctcgcg gcgttcgcga tccagaacag gtaggccttc gcctcctccg ggtatccacg
     1861 atcgagcagc gcaatcacag agcgcgacgc atcccggatc cacacgtgcc ggtagtccca
     1921 gttccgctcg ctcccgatcg cctcggggag ggaggtggtc ggtgcggcga cgacaccgcc
     1981 cgagggagcg aacgtcagga gcttcagcgt gatcgccgag cgctccacgg ccgctcgttg
     2041 ctccggagca ccctggatcc ggttcgacca ctcccgccag cacgccacgg tgtctttgaa
     2101 ggcgcgctcg gcggccgccc cgatcggcgg catgatcccg atgtcgcgat cggcgtacgc
     2161 aagacagagg tacgcgacgt cgccggatcc gatctcgatc cgagcggcgg cggtatcctg
     2221 cgctaccgag aacggggtcg tacaagcggc gaggatgccc cggccgccgg atgtggcagc
     2281 gagggaccgt ccgtgggacc gcaggtccca gcggctaccc ccgaacgggt ctcgcggaga
     2341 gacctccgca tcgagcgcga cccatccttc ctctgcctcg acgcgccgga tcaaccatcg
     2401 gaacggccag agctcagtct ctctgcgcac ggcaggcacg ctcgcgaaca ggtcggtgag
     2461 cgtcgcgatg cccgtcgcgg ttcggaacgt cgtctccagc acgggggttc ccgggaggta
     2521 acgccgttgg gtcccaacga gctcggcggc gcgcatcgtg cagtgcccac cgcccggatc
     2581 gagcaggcgc ccgaacacgg ggtcggcgtc gaagctcggt aggcagagcc agtcgatcga
     2641 gccctcatcc gagaccagag cggcggttcc gcagtcgccg atcaggccgt accgctcgat
     2701 aggaacgtcg gtcgagatgt ccacgaccct ccttaccacg gtcgagtcga ctcggaactt
     2761 gatccagccc tctaggattc cgagtggcca tgcggtgcaa aggggagtca ctcgatgagc
     2821 ccgaccatct cggagatcgc cgaggcgttc tctcgtcacg agttcgaccg aacgttccca
     2881 cacatgggac gatggcatcg agtgggcgca gatcggcgaa ggtcacgtcc ggggcaaggc
     2941 cgacgtcgtt accacctgcc aaaggtcgag tgagtatctt gcaggcgttc gaacgacctt
     3001 cagcagattc aagatcgtgg cggcggagaa ctgcgtcatg atcgatagtc gagccgagta
     3061 cgtcgatgcc gaaggcgagt catctcaggt cgcctcgtgc gacatctacg acttcatcga
     3121 tggatccctc gccgccatca cgacctatgc cgtcgagatc gacagcagcg ctgctgactg
     3181 aagccaccct ctcgatctgc agacacaggg gagaagacga tgaaggcgat cgtccaggag
     3241 cggtacggct cacccgacgt cctcgagctc cgggagatcg agaggccgaa cgtcgaggat
     3301 gacgaggtgc tggtccgggt cagcgcggca tcggtccatg cagatgtctg gcacgccgtg
     3361 cacggggtgc cctacgtcct ccggatcatg ggctccggac tgcgcgcacc gaagcatccc
     3421 gttccgggga cggatctggc cggccacgtc gagtcggtcg gaaggaacgt cacgcggttc
     3481 cgaccgggcg acgaggtctt cggtcagacg ctcaccgcca acctgtggcg caacggcggc
     3541 gcctacgcgg agtacgcggc cgccccggaa gccacgctcg agctgaagcc cgacctcctc
     3601 accttcgaac aggccggagc cgttccgacg tccggatcgc tcgcggtgca ggcggttcgc
     3661 gacgaggggc ggacccgctc gggacagtcg gtcctgatca acggcgccgg tggagcggtg
     3721 gggaccttcg cggtgcagct cgccaaggcg tacggcgcgg aggtgaccgg ggtagatgcg
     3781 ccgggcaagc tcgacgtgct ccggtcgatc ggcgcggacc gagtcgtcga ctacacgcgc
     3841 gaggatttca cgcagagcgg cgagcgctac gacctcatca tcgacatcgc ggggacccgt
     3901 ccgtggtcgg cctccagacg cgcgctcggc tccgagggga cgtacgtcct catcgggcac
     3961 gatcagtacg gaggttcggg ccatcgctgg ttcggaagcc tgggtcggtt cttcaagctg
     4021 atggtggtgt caccgttcgt gagccagctg catcccttcc gcggagcgaa ggatcccgga
     4081 gaccgcttga tcgtcatgaa ggagctgatc gaagccggga agatcacgcc ggtcatcgac
     4141 cggacgttcc cgctgagcga ggtgcccgag gccatccgct acctgggatc gggacaggcc
     4201 ggaggcaaga tcgtcatcac cgtgtgagct ccgaccgcca tctccttgcc ctatcccgcc
     4261 gcgacgaccg ccaagacctc gtccgcgtac cgttcgagct tcgccggtcc gacgcccgag
     4321 atcgcggcga ggtcggccca gtccttgcac cgtcgttcgg cgatctcggc gagggttcgg
     4381 tcgtggaaca cgacgtaggc gggcaccccg tccgcgttcg cgcgtttgcg gcgccattcc
     4441 ttgagccgg
//
LOCUS       DASUYD010000255         9514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_864969, whole genome shotgun sequence.
ACCESSION   DASUYD010000255 DASUYD010000000
VERSION     DASUYD010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 9514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9514
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864969"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1463
                     /locus_tag="VF195_13330"
     CDS             240..1463
                     /locus_tag="VF195_13330"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HEX6581833.1"
                     /translation="MAAYLIVFLVAAGTTFALTPVVRRLAVRFGAIDTPSDRKLHTVP
                     TPTMGGLAMWAGFLVAIGVSRLLPFFDPMNATAVEPLAAVVTCTMMVGLGVIDDRRGT
                     SALAKLTAQIFIAGILILLGVQLAFFVLPGEGLAVLGTDEAVPLTILWVLAAANAINL
                     VDGLDGLAAGMVAIAAATFFLYMVRGADLSASASVAALLSAITVGVCLGFLPWNFHPA
                     RIFMGDTGSMLLGMLVAVATISGVGLNPFPPERGDVAVIALPLLVPLLVLAIPLLDVV
                     LAIVRRTRKGLGIAHADKEHIFHRLMDIGHGHRQTVLLMYLWSALVCGSALAIGVIDG
                     LLVAGAIVAGAVTLFLVTALPRLLERRRNGDTNGAGEEAGAADLPGTAGASMPASGVT
                     TKPAPGAAPADPSAP"
     gene            1616..1834
                     /locus_tag="VF195_13335"
     CDS             1616..1834
                     /locus_tag="VF195_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012922623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX6581834.1"
                     /translation="MSGSRKSDAWSGMGTGWAITSTLIGGMATVGGLGYLLDRVLDTG
                     SGFTGLGIVLGAASAIYIVYLRYGRGDE"
     gene            1831..2250
                     /locus_tag="VF195_13340"
     CDS             1831..2250
                     /locus_tag="VF195_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581835.1"
                     /translation="MTTTVEPERELVRRLLPFAVPALAIAAGAGAVIGGAGAAWSAAI
                     AIVILVLNFVAHARSMAWASRISPMVLMAAGLGGYVVRLAAFTVALLLLDGLAWFSPL
                     AFIAAFAPATIVLLAMEMKLLSGRMQADLWYFPERTT"
     gene            2247..3041
                     /gene="atpB"
                     /locus_tag="VF195_13345"
     CDS             2247..3041
                     /gene="atpB"
                     /locus_tag="VF195_13345"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEX6581836.1"
                     /translation="MIAAVLAEDFHPPGTQDFVFDCYFSVDLFGIEICFNFIIALVVA
                     SVLVYMLLFFFAFRKPRTVPGKLQSLMELGLEFVREQIALPMLGPAADRFLPLLVTLF
                     FFIFFMNMFEVAPWVNFSASSRLAFPLVLAVIAWVTYNAVGIKKHGFFGYLKFTCIIP
                     SAPAILRYSLLMVIEFFSNIVIRPITLTIRLAANFVAGHFLLALAFLGTEFFFLNGGL
                     SYLGLAVTAPISVALVAFEVFVAILQAFIFAILTASYIGGVLAEEH"
     gene            3081..3326
                     /gene="atpE"
                     /locus_tag="VF195_13350"
     CDS             3081..3326
                     /gene="atpE"
                     /locus_tag="VF195_13350"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEX6581837.1"
                     /translation="MLGLLAQAASDFSGLETIGKGLIIGLAAIGPGIGLGILIGKSVE
                     AMARQPEYANQVRTTMFIGIGLIEALALFGIAFSFII"
     gene            3337..3945
                     /locus_tag="VF195_13355"
     CDS             3337..3945
                     /locus_tag="VF195_13355"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF004412.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEX6581838.1"
                     /translation="MFALIASILAQAEESAEEVSDAKDLYPAVGELIVGLIAFAVLFF
                     FTWKWVIPSFKKVLEERREKIQGEMERAEATRKEADKLQEEYRSQLNGVRDEANRIIE
                     ESRATADQLRRDLQVKAEEEAQGIVARAQEEIRAERDRVFQELRAQVGSIAVEIAGRV
                     VGQSLDEETHQRLIDEFIDEIASGAAANGNGNGKAGDSGSVD"
     gene            3946..4488
                     /gene="atpH"
                     /locus_tag="VF195_13360"
     CDS             3946..4488
                     /gene="atpH"
                     /locus_tag="VF195_13360"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX6581839.1"
                     /translation="MAVDDTVRGYAHALLSIAEAEGVLDRAQDELYAFAKAVERNGDL
                     REALTDAVLPPERKKAVIDELLGERAHPTTATLVGFLVDAGQARRIGAIAEELAKEAA
                     HRSERTLAEVRVAVPLSDAKKSLLADALERATGHPVELKIDVDPSVIGGVVARVGDEV
                     FDGSLASRLTEAKQYLTGSV"
     gene            4494..6239
                     /gene="atpA"
                     /locus_tag="VF195_13365"
     CDS             4494..6239
                     /gene="atpA"
                     /locus_tag="VF195_13365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017954664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEX6581840.1"
                     /translation="MAVDAERFDPNDIADALRKHVETWTPSVERAELGRVIEAGDGIA
                     RVSGLPGAMANELLEFPGGVLGLAFNLDVGEIGCIVLGDASHIQEGDPVKQTGQVLSV
                     PVGDGFLGRVVNALGEPIDDKGPVQSQDRRILEVQAPNVVNRQPVKEPLYTGITAIDA
                     MTAIGRGQRELLIGDRQTGKTAVAVDAIIAQKELWATGDPKKRVKCIYVAVGQKSSTV
                     AEVVATLEENGALEYTVVVNAPASDPAPFQYIAPYSGAAFGAHWMYNGEHALIVYDDL
                     SKQATAYRTLSLLLRRPPGREAFPGDVFYLHSRLLERAAKLSDELGGGSLTALPIIET
                     KGNDISAYIPTNVISITDGQIFLEPDLFFAGVRPAINIGISVSRVGGSAQTKAMKKVA
                     GRLRLDLAQFRALEAFAQFGSELDKASQQQLARGARVVEVLKQPQYEPLPVEREVVMI
                     WTATGGYLDPFPVEDARRFVEEFTTFLDSRTDVLGAIKETGDMTDETEATLKESVEAF
                     AQSFQTSEAGAGSEAGRGAGTPPDEVKPDIGWDRMSSVDDDEEEEQDGGPSLAEEDDV
                     EGQVPLPDEEDEEGA"
     gene            6243..7136
                     /locus_tag="VF195_13370"
     CDS             6243..7136
                     /locus_tag="VF195_13370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEX6581841.1"
                     /translation="MGAQLRIVRRRIRSVQSTKKITRAMELIASSRIVKAQQRVESSR
                     PYAIQLTKAMEDLARQTGSVQHPLLEDRENPSKAGILVVTSDRGLAGAYNANVLKVAE
                     GVVRNVRSRGLEPVLYVIGKKGVGYFRFRGVPMRKSWQGFSEVPPYEEAERVGRVLIK
                     DFADEEIDELHAAYTDFRSAFTLRATTKRFLPIAPEEVTGKGLGVVHPEYLFEPEPEE
                     ILDHLLPQYVVTKVYAGLLESAASENAARRRAMKAATDNAEDLVKILTRQANRARQDE
                     ITTEIMEIVGGAESMAAGSEG"
     gene            7139..8590
                     /gene="atpD"
                     /locus_tag="VF195_13375"
     CDS             7139..8590
                     /gene="atpD"
                     /locus_tag="VF195_13375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX6581842.1"
                     /translation="MPETPKNEPRNEKGHPEGRVVRVIGPVIDVEFPPSELPEVHTAL
                     TVDLTLEGASTTITCEVAQHIGASIVRTIALKPTDGLVRGTPVKNTGSPITVPVGEGV
                     LGHVYNVIGEPLDADPSEIHASDRWPIHREPPQFSELEPKKQMLETGIKVIDLLEPYV
                     QGGKIGMFGGAGVGKTVIIQEMIRRQAKEHGGVSVFAGVGERTREGNDLFLEMTESGV
                     INNTALVFGQMDEPPGVRLRVALSALTMAEYFRDVERKDVLLFIDNIFRFTQAGSEVS
                     TLLGRMPSAVGYQPTLADEMGELQERITSAGGRSITSLQAIYVPADDITDPAPHTAFA
                     HLDATTVLSRDIAALGIYPAVDPLDSTSRILDARYVGQDHYDTARRVQEILQRYRDLQ
                     DIIAILGIDELSEEDKVIVSRARKIQRFLSQPFFVAEQFTGLPGKYTPIDETIASFKA
                     LCEGEYDHLPEQAFFLVGGIEEAVEQAKTLEAA"
     gene            8590..8988
                     /locus_tag="VF195_13380"
     CDS             8590..8988
                     /locus_tag="VF195_13380"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEX6581843.1"
                     /translation="MALEVHVVTPEREVWSGQATEVIAHGVDGDVGILAGHAPLLVHL
                     AIGPLQIQEEDGTWLKAVVDGGFLHVSTEEGASRVDVLATNAQLEAEIDVDAVTRRLE
                     ELRSSAEGHDEQVKTEIARAEARLTLNGSD"
     gene            9037..>9514
                     /locus_tag="VF195_13385"
     CDS             9037..>9514
                     /locus_tag="VF195_13385"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377618.1"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX6581844.1"
                     /translation="MDRLFVTGGARLAGSVRISGAKNSALKLMAASLLAPGRSVLRNV
                     PRIQDCFTMGEVLEQLGARISWEDGGVAVDATHLSSVEAPYELVRRMRASIAVLGPLL
                     ARTGEARGAMPGGDNIGSRPIDLHLDGLRRMGARIDAEHGFLVATADRLSAASITLD"
BASE COUNT         1536 a         3148 c         3297 g         1533 t
ORIGIN      
        1 cgacgctcgg gatggacgag gcagagatgg gcgaggtggc gtcgatcatc ggcgacgtcc
       61 tgaagcaccc ggacgacggg tccgtgaagg agaagtccgg gcagcgcgtc cgggacctga
      121 tggaacggtt cccgctctac ccttgatgac gtgcccgtgc cgatggtgcc ctgacgttct
      181 ggccgtgggc agggccgccg gttgccgggt ggcgaggacc ccagcacact gaggccaccg
      241 tggccgcgta tctgatcgtg ttccttgtcg cagcggggac cacgttcgcg ctgacgcccg
      301 tggtgcggcg cctcgccgtc cggttcggcg cgatcgacac gccgtccgat cgcaagctcc
      361 acacggtccc cacgccgacg atgggtggcc tcgcgatgtg ggccggcttc ctcgtggcga
      421 tcggggtctc ccggctcctg ccgttcttcg atccgatgaa tgcgaccgcc gtcgagcctc
      481 tcgcggcggt cgtcacgtgc acgatgatgg tcggtctcgg cgtgatcgac gaccggcggg
      541 gcacctccgc gctcgcgaag ctcacggccc agatcttcat cgccggcatc ctgatccttc
      601 tcggcgtcca gctcgcgttc ttcgtcctcc cgggcgaggg gctcgccgtg ctcgggaccg
      661 acgaggccgt gccgctcacg atcctctggg tactggcggc agcgaacgcg atcaacctcg
      721 tggacgggct ggatgggctc gcggccggca tggtggcgat cgccgccgcc acgttcttcc
      781 tctacatggt ccgcggagcc gatctctccg cgtccgcgtc cgtcgccgcg ctcctgtccg
      841 cgatcacggt cggcgtctgc ctgggcttcc tcccctggaa cttccatcct gcgaggatct
      901 tcatgggcga cacggggtcg atgctcctcg ggatgctcgt ggcggtcgcc acgatctcgg
      961 gcgtcggcct gaaccccttc ccgccggaac ggggagacgt cgcggtgatc gcgttgccgc
     1021 tgctcgtgcc tctgctggtc ctcgcgatcc ccctgcttga cgtcgtcctc gcgatcgtgc
     1081 gccgcacgcg gaaggggctc gggatcgccc acgcggacaa ggagcacatc ttccatcggc
     1141 tcatggacat cggtcacggt caccggcaga ccgtgctgct gatgtacctc tggtccgcgc
     1201 tggtctgcgg ctccgcgctc gcgatcgggg tcatcgacgg cctgctggtc gccggcgcga
     1261 tcgtcgcggg cgcggtcacg ctcttcctcg tgacggcgct gcctcgtctc ctggagcgtc
     1321 ggcgcaacgg ggatacgaac ggggcgggcg aggaggcagg ggcggcggat ctccccggga
     1381 cggccggagc gagcatgccc gcgtcgggcg tgaccacgaa gcccgcgccg ggcgcagctc
     1441 ccgccgatcc gagcgcccct tgacgggcgc gatcgggtcg ggtcgacgtc cggaccatgt
     1501 gaatcctttc acaagctcgc cgacacagct tccggaaacg gcctctgacc agcggaaacg
     1561 caggattccg cggattgacg gcatgtgaag gggccgacta gagtgtccgc ctccgatgag
     1621 cggatcccgc aagagcgatg cgtggtccgg catggggacc ggctgggcga tcacctccac
     1681 cctcatcggg gggatggcca ctgtcggagg gttgggatat ctgctcgacc gggtcctcga
     1741 tacgggatcc gggttcaccg ggctcggcat cgtcctcggg gcggcgagcg cgatctacat
     1801 cgtgtatctg cggtacggca ggggagacga gtgacgacca cggttgagcc ggagcgcgag
     1861 ctggtgcgcc ggctcctgcc gttcgccgtg cccgccctcg cgatcgcggc aggagccggc
     1921 gccgtgatcg gaggagccgg tgctgcctgg tcggcggcga tcgcgatcgt catcctggtg
     1981 ctcaacttcg tcgcgcacgc cagatcgatg gcatgggcct cgcggatctc gcctatggtc
     2041 ctgatggccg ccggattggg cggctacgtc gtgcgtctgg cggctttcac ggtcgccctc
     2101 ctgctcctgg acgggctcgc ctggttctcc ccgctcgcgt tcatcgccgc gttcgcgccg
     2161 gccacgatcg tgctcctcgc gatggagatg aagctgctca gtggacgaat gcaggccgac
     2221 ctctggtatt tcccggagcg gaccacgtga tcgcggcagt cctcgccgag gatttccacc
     2281 cgccagggac gcaggacttc gtcttcgact gctacttcag cgtcgacctg ttcggcatcg
     2341 agatctgttt caacttcatc atcgcgctcg tcgtggcgtc ggtgctggtc tacatgctct
     2401 tgttcttctt cgcattccgc aagccacgga ccgtgccggg caagctccag tcgctgatgg
     2461 agctgggcct cgagttcgtg cgcgagcaga tcgcgttgcc gatgctcggg ccggccgccg
     2521 atcggttcct cccgctgttg gtcaccctgt tcttcttcat cttcttcatg aacatgttcg
     2581 aggtcgcccc gtgggtgaac ttctcggcga gcagccgcct ggcgttcccg ctcgtgctcg
     2641 cggtgatcgc ttgggtcacc tacaacgccg tcgggatcaa gaagcacggg ttcttcggct
     2701 acctgaagtt cacgtgcatc atcccgagcg caccggcgat cctgcgctac tcactcctga
     2761 tggtgatcga gttcttctcg aacatcgtga tccgcccgat caccctgacc atccgactcg
     2821 cagcgaactt cgttgccggg cacttcctgc tcgcgctcgc gttcctcgga accgagttct
     2881 tcttcttgaa cggaggattg agctacttgg ggctcgcggt gaccgccccg atctccgtgg
     2941 cgctcgttgc gttcgaagtc ttcgtggcga tcttgcaggc attcatcttc gccatcctga
     3001 ccgcgtcgta catcggcggg gtcctggcgg aagagcacta gacaaggcca agaccaaggc
     3061 caaagatcaa ggaggaatgg atgttggggt tgctcgcaca agccgccagt gatttctcgg
     3121 ggctggaaac gatcggcaag gggctcatca tcggtctcgc ggccatcggt ccgggcatcg
     3181 gactcgggat cctgatcggg aagtcggtcg aagcgatggc tcggcagccc gagtacgcga
     3241 accaggtccg gaccaccatg ttcatcggca tcggcttgat cgaggcgctc gcgctgttcg
     3301 gcatcgcgtt ctcgttcatc atctgagggg cggacgatgt tcgcgttgat cgcatcgatc
     3361 ctcgctcaag ccgaggagag cgccgaggaa gtctcggacg cgaaggacct ctaccccgcg
     3421 gtaggcgagc tcatcgtcgg gctgatcgcc ttcgccgtcc tgttcttctt cacctggaaa
     3481 tgggtgatcc cgagcttcaa gaaggtgctc gaggagcgcc gggagaagat ccagggagag
     3541 atggagcgcg ccgaggccac ccgcaaggag gccgacaagc tccaggagga gtaccggtcg
     3601 cagctcaacg gcgtccggga cgaagcgaac cgcatcatcg aggaatcccg cgcgaccgcg
     3661 gaccagctgc ggcgcgacct ccaagtcaag gccgaggagg aggcccaggg catcgtggcc
     3721 cgggcgcagg aggagatccg cgccgaacgc gatcgcgtgt tccaggagct ccgcgcgcag
     3781 gtcggatcga tcgcggtgga gatcgccggg agggtcgtgg ggcagtccct cgacgaggag
     3841 acgcatcagc gcctgatcga cgagttcatc gacgagattg cctccggcgc ggccgcgaac
     3901 gggaacggca acgggaaggc cggagacagc gggagtgttg actgagtggc ggtcgacgac
     3961 acggttcgtg ggtacgccca cgcgcttctg tccatcgcgg aagcggaggg tgtgctcgat
     4021 cgtgcccaag acgagctcta tgccttcgcg aaggccgtag agcgcaacgg cgacctacgc
     4081 gaggcgctca ccgacgccgt gctcccgccc gagcgcaaga aggcggtgat cgacgagctc
     4141 ctgggagagc gcgcgcaccc caccaccgcg acgctcgtcg gattcttggt cgacgccggc
     4201 caggcgcgcc ggatcggcgc gatcgctgag gagctggcga aggaggccgc ccaccgatcg
     4261 gaacggacgc tcgccgaggt gcgcgtcgcc gttcctctga gcgatgcgaa gaagtcgttg
     4321 ctcgccgacg cgctcgagcg ggccacgggc catccggtgg agctgaagat cgacgtcgac
     4381 ccttcggtca tcggcggcgt ggtggcgcgg gtgggcgacg aagtgttcga cggctcgctc
     4441 gcgagccgat tgaccgaggc gaagcagtac ctcacaggga gcgtgtgatc cggatggcag
     4501 tggacgcaga gaggttcgac ccgaacgaca tcgcggatgc cctccgcaag cacgtcgaga
     4561 cctggacgcc gtcggtcgag cgggccgagc tcggccgcgt gatcgaggcc ggcgacggca
     4621 tcgcccgcgt gtcgggcctt cccggcgcga tggccaacga gctgctggag ttccccgggg
     4681 gcgtcctcgg gctggcgttc aacttggacg tcggcgagat cgggtgcatc gttctcgggg
     4741 acgcctcgca catccaggag ggagacccgg tcaagcagac gggccaggtc ctgtccgtgc
     4801 cggtcggcga tggattcctc ggacgggtcg tgaacgcgct cggggagccg atcgacgaca
     4861 agggcccggt ccaatcgcag gaccgccgga tcctcgaggt ccaagccccc aacgtcgtca
     4921 accggcagcc ggtgaaggag cccctctaca cggggatcac ggcgatcgac gcgatgacgg
     4981 cgatcggacg gggccagcgt gagctgctga tcggggaccg gcagaccggg aagaccgcgg
     5041 tcgcggtgga cgcgatcatc gcccagaagg agctctgggc gaccggggac ccgaagaagc
     5101 gcgtgaagtg catctacgtc gcggtcgggc agaagtcctc gacggtcgcc gaggtcgtgg
     5161 cgaccctgga ggagaacggc gcgctcgagt acacggtcgt cgtgaatgcg ccggcctcgg
     5221 acccggcccc gttccagtac atcgctccgt actccggtgc cgccttcggg gcgcactgga
     5281 tgtacaacgg cgagcacgcg ttgatcgtgt acgacgacct ctcgaaacag gcgaccgcgt
     5341 atcgcacgct ttcgctgctg ctccgccggc cgcccgggcg cgaggcgttc cccggcgacg
     5401 tcttctacct gcactcgcgg ctgctcgagc gcgccgccaa gctctcggac gagctcggag
     5461 gcggttcgct caccgcgctc ccgatcatcg agaccaaggg caacgacatc tcggcctaca
     5521 tcccgaccaa tgtgatctcg atcaccgacg ggcagatctt cctcgagccg gacctgttct
     5581 tcgcgggcgt ccgtcccgcg atcaacatcg gcatctcggt ctcgcgggtc ggcgggagcg
     5641 cgcagacgaa agcgatgaag aaggtcgccg gccgcctccg gctcgacctc gcccagttcc
     5701 gggccctcga agcgttcgcg cagttcggct ccgagctgga caaggcctcg cagcagcagc
     5761 tcgcgcgcgg tgcacgcgtc gtggaggtcc tgaagcagcc ccagtacgaa ccgctccccg
     5821 tcgaacgcga ggtcgtgatg atctggacgg cgaccggggg gtacctcgat ccgttccccg
     5881 tcgaggacgc gaggcgcttc gtggaggagt tcacgacgtt cctggactcc aggaccgacg
     5941 tcctcggcgc gatcaaagag acgggcgaca tgaccgacga gacggaggcg acgctgaagg
     6001 agtcggtcga ggcgttcgcg cagtcgttcc agacgagcga agcgggggcc ggcagcgagg
     6061 ccggccgcgg cgccggcacg ccgcccgacg aggtcaagcc cgacatcgga tgggaccgga
     6121 tgtcctcggt ggatgacgac gaagaagagg aacaggacgg gggcccgtca ctggccgaag
     6181 aggacgacgt cgagggccag gtgcctctgc cggacgagga agacgaggag ggcgcctagc
     6241 cgatgggagc ccagctccgg atcgtccgcc ggcggatccg ctcggtccag tcgacgaaga
     6301 agatcacgcg cgcgatggag ctcatcgcct cctcgcggat cgtgaaggcg cagcagcgcg
     6361 tggagtcgtc ccgcccgtac gcgatccagc tgaccaaggc gatggaggat ctcgcgcggc
     6421 agacgggctc ggtacagcac ccgctgctcg aggaccgcga gaacccttcg aaggcgggca
     6481 tcctggtcgt gacgtcggac cgggggctcg cgggcgcgta caacgcgaac gtgctgaagg
     6541 tcgcggaggg cgtggtccgc aacgttcgca gccgagggct ggagcccgtc ctgtacgtga
     6601 tcggcaagaa gggcgtcggc tatttccggt tccggggcgt gccgatgcgc aagagctggc
     6661 aggggttcag cgaggtcccg ccgtacgagg aggcggaacg cgtcggccgc gtcctgatca
     6721 aggacttcgc ggacgaggag atcgatgagc tccacgccgc ctacaccgac ttccgctccg
     6781 cgttcacgct ccgcgcgacg acgaagcggt tcttgccgat cgctcccgaa gaagtcaccg
     6841 ggaagggcct cggcgtcgtc caccccgagt acctgttcga gccggagccg gaggagatcc
     6901 tggatcacct gctcccgcaa tacgtggtga cgaaggtcta cgcagggctg ctcgaatcgg
     6961 cagcgtccga gaacgctgcc cggcgccgcg cgatgaaggc agcgaccgac aacgcggaag
     7021 acctggtgaa gatcctgacg cggcaagcga accgggcacg ccaggacgag atcacgaccg
     7081 agatcatgga gatcgtgggc ggagccgaat cgatggctgc cggctcggag ggatgacgat
     7141 gccagagacg cccaagaacg agccaaggaa cgagaagggt caccctgagg gacgagtggt
     7201 gcgggtgatc ggcccggtga tcgacgtgga gttcccaccg tccgagcttc ccgaggtcca
     7261 cacggcgctc accgtcgacc tgaccctgga gggagcgtcg acgacgatca cctgcgaggt
     7321 cgcgcagcac atcggcgctt cgatcgtccg cacgatcgcc ctcaagccga ccgacggtct
     7381 cgtacgcggc acccccgtca agaacacggg gtcgccgatc accgtccccg tgggggaggg
     7441 cgtgctcggt cacgtctaca acgtgatcgg cgagccgctc gatgcggacc cgtccgagat
     7501 ccacgcgagc gaccgctggc cgatccaccg cgagccgccg cagttctcgg agctcgagcc
     7561 caagaagcag atgctcgaga ccggcatcaa ggtcatcgac ctgctcgagc cctacgtaca
     7621 gggcgggaag atcgggatgt tcggcggcgc cggcgtcggc aagaccgtga tcatccagga
     7681 gatgatccgc cggcaggcga aggagcacgg cggcgtgtcg gtcttcgccg gcgtggggga
     7741 gcggacccgc gagggcaacg acctgttcct cgagatgacc gagtccggcg tgatcaacaa
     7801 cacggcgctc gtcttcgggc agatggacga gccgcccggc gtgcgcctcc gggtggccct
     7861 ctcggccctg acgatggccg agtacttccg cgacgtcgag cgcaaggacg tgctgctctt
     7921 catcgacaac atcttccggt tcacacaggc gggctccgag gtctccacgc tgctcggtcg
     7981 gatgccgtcg gcggtcggct accagccgac cctcgccgac gagatggggg agctccagga
     8041 acggatcacc tcggccggtg gccgatcgat cacatcgctg caggcgatct acgtccctgc
     8101 ggacgacatc acggacccgg cgccgcacac ggcgttcgcc cacctcgacg ccacgaccgt
     8161 cctctcgcga gacatcgcgg cgctcggcat ctatccggcg gtggacccgc tcgactccac
     8221 ctcgcggatc ctcgacgccc ggtacgtcgg gcaggatcac tacgacacgg cgcgccgcgt
     8281 ccaggagatc ctccagcggt accgcgacct ccaggacatc atcgcgatcc tcgggatcga
     8341 cgagctgtcc gaggaggaca aggtgatcgt gtcgcgggcc cgcaagatcc agcggttcct
     8401 ctcacagccg ttcttcgtcg cggagcagtt cacggggcta ccgggcaagt acacgccgat
     8461 cgacgaaacg atcgcgtcgt tcaaggcgct ctgcgaaggc gagtacgacc acctccccga
     8521 gcaggcgttc ttcctcgtcg gcgggatcga ggaggccgtc gagcaggcga agaccctcga
     8581 ggccgcgtga tggcgctcga ggtccacgtc gtcacgccgg agcgggaggt gtggtcgggg
     8641 caggccaccg aggtgatcgc ccacggggtc gacggcgacg tcggcatctt ggccggacat
     8701 gctccgctcc tcgtccacct ggcgatcggg cctctccaga tccaggagga ggacggcacc
     8761 tggctcaaag cggtggtcga cggcggcttc ctgcacgtat ccacggagga gggcgcatcc
     8821 cgggtggacg tgctcgccac gaacgctcag ctcgaggccg agatcgacgt cgacgccgtc
     8881 acgaggcgcc tcgaggagct gcgatcctcc gccgagggtc acgacgagca ggtcaagacc
     8941 gagatcgcgc gggccgaagc caggcttacc ctgaacggtt ccgactgacc gtcgccgccg
     9001 ggccgtccct caggccgccc ccggtacgat gcgcagatgg accgtctctt cgtgacgggg
     9061 ggcgctcgcc tcgccggttc cgtccgcatc tcgggcgcca agaactcggc cctgaagctg
     9121 atggcagcgt cgttgctcgc acccggacgt tccgtgctgc gcaacgttcc tcgcatccag
     9181 gactgcttca cgatgggcga ggtcctcgag caactcggcg cccggatctc gtgggaggac
     9241 ggaggcgtcg cggtggatgc gacccacctc tcatccgtgg aggcgccgta cgagctggtt
     9301 cggcgtatgc gggcctcgat cgcggtgctc ggcccactcc tcgcccgtac cggtgaagcg
     9361 cgcggggcga tgcccggcgg cgacaacatc ggctcacgtc cgatcgacct ccatctcgac
     9421 gggctccgac ggatgggcgc gcggatcgac gccgagcacg ggttcctggt cgcgacggcg
     9481 gaccggctct cggccgcgtc gatcacgctc gatt
//
LOCUS       DASUYD010000256        10544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1531282, whole genome shotgun sequence.
ACCESSION   DASUYD010000256 DASUYD010000000
VERSION     DASUYD010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10544
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1531282"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1197)
                     /locus_tag="VF195_13390"
     CDS             complement(10..1197)
                     /locus_tag="VF195_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HEX6581845.1"
                     /translation="MRVLLFTGKGGVGKTTVAAATAVRAADAGLRTLVMSTDPAHSLG
                     DSFDEEIGSHPTQIAERLWAQQIDAQERLEDNWREIQDYMIQLMNWAGTETIQAEELT
                     VLPGLDEIFSLIDVKTHVESGAYDVLIVDCAPTAETLRLLSLPDVMQWYIERIFPVER
                     RIVKTVRPLVSRVTSLPIAGDRVFDAVERLHRNLDAVKKILTDEATSSVRLVVNPEKM
                     VIAEARRTYTYLGLFGYRVDAVVVNRVLPPDVTDPYFGKWKDIQAEHLATVHESFEPV
                     PILTSRLFDREMIGVRLLEEMATEIYGDLDAADILYRDDPIRVRKRGTGYVLVMRLPF
                     ASREDMDIHRRGEELFIRVGAYKRNLILPQSLKRMSVREANFAGDHLEILFGREPRPV
                     ERS"
     gene            complement(1201..1641)
                     /locus_tag="VF195_13395"
     CDS             complement(1201..1641)
                     /locus_tag="VF195_13395"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581846.1"
                     /translation="MEHAEGSIVIDAPLEDVMEVLEDYEAYPEWAEVRTAQIRERGEG
                     GRAREVAFEVDVPVLGKATYTLSYRYAPGDTGLSWTTKEARGAIRDIRGEYLLNEISD
                     SETKVTYRLAVEIGILVPGFVRTEGAKRVIENALERLKRRVEMG"
     gene            complement(1689..2306)
                     /locus_tag="VF195_13400"
     CDS             complement(1689..2306)
                     /locus_tag="VF195_13400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581847.1"
                     /translation="MSADAEWCGQCFSSLRAPTPEPATAAVPVVARPQEGKEQGRPGE
                     REEPAKLTWPCPTCGSQNDIALDLCSVCGTSFGQLLREERAAPTVSPRDAFLWSLTFP
                     GVGHAKAGRALDGIARGTLFVLSFGLALVIVLSGVSSPPVFGVVVLLLGSAMVLYLGS
                     AAEAYRIAGGGSPFVSSRALLWATVALIMLTVSLLAVSVVSVNSR"
     gene            complement(2416..3549)
                     /locus_tag="VF195_13405"
     CDS             complement(2416..3549)
                     /locus_tag="VF195_13405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX6581848.1"
                     /translation="MDIPRTLVVTNDFPPRVGGIQRTLESLCRELPHDRISVIAPSCE
                     GSERFDASVPFTVVRERRRFVWPSPGVVRRLSAEVDRSGAEVVLFGDAFPLALMASQL
                     KARGTPSVVLAHGFDYWLSTVPIAHAVMKRMTARASRVAGCSEFISRRVRTAVPKHVP
                     VSVLHPGADVERFRPDLPTADIRERHGLGDRPLIVCVSRLVPRKGQDVLIRAMRSIQR
                     RVPEAGLLIVGSGPYGSTLRALAERAPTRSVTFVGEVSEEDLPRYYAVGDVFAMPCRT
                     RLAGLEVEGWGNVFIEAAACGRPVVVGDSGGSREALVHGATGLLVNGAEVSGVADAVA
                     TLLEDPAYARRLGKAGRARVEHEHTWARVAERLAGWLREAIAA"
     gene            3642..4463
                     /locus_tag="VF195_13410"
     CDS             3642..4463
                     /locus_tag="VF195_13410"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581849.1"
                     /translation="MLSGWGVTVVGGTDARSWLHDLVTADVEGLPDRTSRRCLLLTPT
                     GRIRADFHIAALDGSYLLLQAPGQPDRVDAILRPYVLSSDVEVTDVGDRSEVVAVLDG
                     SAVEGGSGSVVLSPSVLGPGYDVVVASGDASARLRERLRAGGLTEADPGDLEARRIRR
                     GEPRMGVDFGPDALPAEAGLEDTIDFSKGCFLGQESVAKVRNLGHPPRVLLHVRSGAP
                     IARGARVVAGATVVGEVTSAVEDPDGVAAIVRVRWDAATEQLATDAGPLSLQRVE"
     gene            4599..4877
                     /locus_tag="VF195_13415"
     CDS             4599..4877
                     /locus_tag="VF195_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HEX6581850.1"
                     /translation="MRIDRLPTVDVDVLPDDPSPPDVWQERSACFGIDPDVFFPISEE
                     EAGPALAFCSACTIRTECLSWALKNGERYGVWGGLTEQQRRRLTRQVA"
     gene            4971..7106
                     /locus_tag="VF195_13420"
     CDS             4971..7106
                     /locus_tag="VF195_13420"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HEX6581851.1"
                     /translation="MDAVVPAPHRRRSFSLIRLVAGAAAATMLILSLPSPAAAAPTPS
                     ITISDVTVTEGTGAAPVTASFTIQAAPPPRVCCALQVGWATAPGSASAPGDFTSSSGT
                     VSLTKSSSSRVIGVPIVGDAADEPNEAFVVNLSNLVGSPGTIGDAQGVATIADDDGPP
                     SLSVNDISATEGNAGTTTATFTVSLSAASGKTVAFDWTTAAGTAAADVDYVTASGSRT
                     IAAGATTATIAITVNGDVVDEVDETFTLALSNPANAAIADGSALGTISDDDAPPALSV
                     NDVSVTEGNAGTTTATFTVSLSAASGKTVTVGWSTADADATQPVDYTAASGTLTFVPG
                     DASETIVVSVNGDLVAELDEILDVTLTAPSNATLADAAGIGTIVDDELLPVIDIDEPM
                     ALEGSGSIVFTASLSHQSASPVTVDWSTAAGTATNGIDYVETIGTLVFAPLDISETVE
                     ITVNEDGTFEHGETMAVNLSNASGAPIGNAQGIGTIANDDDAPGLSVSDVSVLEGNTG
                     QRMLIFSVSLTGDTDIDATFDFATAGLTATQGTDHADAAGTLTIDAGDTGATIDVIVN
                     GDATYESDETLSVTLSDPASAVLVDSAAIGTIRNDDKAPTTVTVRIARAPTRIVATGI
                     LEHAHPGLRITATLFRKAHGRFLKVTVKTARVRDLRDRDGDGNPDGAYTAKFLRPRWK
                     GLYKVAVRFKGSAAYKPCTRSKIFRLSAT"
     gene            7238..8887
                     /locus_tag="VF195_13425"
     CDS             7238..8887
                     /locus_tag="VF195_13425"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HEX6581852.1"
                     /translation="MLSVPIQTTLAEGVPLKEVDFVVVDLETTGGSPRDSRITEIGAV
                     RVLGGERIGSFQALVNPGEAIPPFISHLTGIDDLLVRQEPPIEAVLPAFLEFARGAIF
                     VAHNARFDFSFLNENLGRLEYPLLEGPPVCTAKLARRVVWPEVPNVRLRTLARYFRTR
                     VQPIHRALDDAMACAEVLDQLLDLGARLGILTLGDLRQAVTARGRPHYGKIRLADDLP
                     RAAGVYRFVGRDDRVLYVGKARDLRARVKSYFYGDGRKKVENLLGEVHRVDGEETPGG
                     ELEALVLEARLIRRHEPKYNRRGTTWRRFAYLKLDPTEAFPRLKVVHRAGADDGCAYL
                     GPFSGSGAARLAKEALEEIVPLRRCTRSMGARTRFAPCALAGIGRCTSPCDGGIDPER
                     YGELVRDLLSSLESPDGLLGALEARMRRLADAERFEEAASVRDRLRALAWALHRSRQD
                     AWLVGAGHLELGTEGERRLAFVGGALARANDTDVEPIPEPCPRERADELSAVRSWLAR
                     NPVRLIRCDVPLSEPVDGGARIAKIVRWTNKDPERPRASRR"
     gene            8900..9424
                     /locus_tag="VF195_13430"
     CDS             8900..9424
                     /locus_tag="VF195_13430"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX6581853.1"
                     /translation="MGPAVELLEREMSEAFHDMRQRLHGLADEEFSWEPISGSWTIFR
                     TEDGRWDHHYEEPDPVPAPFTTIAWRLTHLAMCKVMYHEHAFGPGALTWRTIETPGTV
                     ADAIAMLDAGHALLTQDLAEVDEADLERPVRTNWGELWPVWRIFWTMIQHDAHHGGEI
                     GALRDLYAGTRAKG"
     gene            9483..>10544
                     /locus_tag="VF195_13435"
     CDS             9483..>10544
                     /locus_tag="VF195_13435"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX6581854.1"
                     /translation="MEHAVILEGARTPIGKFLGSFAATPVVELGATASREALRRAGVS
                     GDQIDQTIMGHARQAGNGPNTGRQVSIHAGVPEEVSAYNVNIACGSGMKAVQLGAQQI
                     ALGDSEVVLAGGMENMSRVPFLLDRMRTGYRMGNATVHDAMYRDGLLDPLCGLLMGET
                     AENLVDRYGLSREEQDGFALESQAKAAQGTDARAAEMFPLEANDPSGKGTLTIAEDEH
                     PRPGTTADGLARLSPVFREGGSVTAGNAAGITDGAAAMILMSASRARAEGREPLARIA
                     GMSWAGVPPEIMGIGPVPATKKVLEKTGLSLADIDLIEINEAFAAQVLACERELKFDR
                     DRLNLLGGGISLGHPIGMSG"
BASE COUNT         1673 a         3751 c         3535 g         1585 t
ORIGIN      
        1 cggccatggt cagctccttt cgaccggacg gggctcccgt ccgaacagga tctcgagatg
       61 atcgcccgcg aagttcgcct cgcggaccga catccgcttg agcgactgcg gcaggatcag
      121 gttccgcttg tacgccccca ctcggatgaa cagctcctcc ccccgccgat ggatgtccat
      181 gtcctcgcgg gatgcgaacg ggagccgcat cacgaggacg taaccggtcc cgcgtttccg
      241 tacgcggatg ggatcgtccc ggtacaggat gtcggcggcg tccaggtctc cgtagatctc
      301 ggtggccatc tcctcgagca gccggacgcc gatcatctcc cggtcgaaga ggcgcgacgt
      361 gaggatcggc acgggctcga aggactcgtg gacggtggcg aggtgctccg cttggatgtc
      421 cttccacttg ccgaagtacg gatcggtcac atcgggtggg aggacgcggt tcacgaccac
      481 cgcatccacg cgatagccga acagaccgag atacgtgtac gtgcgccgcg cttcggcgat
      541 gaccatcttc tccggattga ccaccaggcg gaccgacgac gtggcctcgt cggtgaggat
      601 cttcttcacc gcgtcgaggt tgcggtggag ccgctccacg gcatcgaaca cgcggtcgcc
      661 cgcgatcgga agtgacgtca cccgcgacac gagcgggcga accgtcttca cgatccttcg
      721 ctccacgggg aagatccgct cgatgtacca ctgcatcacg tcggggaggg agagcaaccg
      781 gagcgtctcg gccgtcggcg cgcagtccac gatcaacacg tcgtacgctc cgctctcgac
      841 gtgcgtcttg acgtcgatca gtgagaagat ctcatcgaga ccggggagca cggtcagctc
      901 ctccgcctgg atcgtctccg tccccgccca gttcatcagc tggatcatgt agtcctggat
      961 ctctcgccag ttgtcctcga gccgctcctg ggcatcgatc tgctgcgccc agagccgctc
     1021 ggcgatctgc gtggggtgcg acccgatctc ctcgtcgaag gagtcaccga gggaatgggc
     1081 ggggtcggtg gacatcacca gcgtccgcag accggcatcg gctgcgcgga ccgcggtggc
     1141 ggcggcgacg gtcgtcttgc cgacaccgcc cttcccggtg aacaggagga cccgcatctc
     1201 tcagcccatc tccactcgcc gcttgagccg ctccaaggcg ttctcgatga cccgcttggc
     1261 gccctccgtc cgaacgaacc cgggaacgag gatgccgatc tcgaccgcga gccggtaggt
     1321 caccttggtc tcggaatcgg agatctcgtt caggagatac tcgccgcgga tgtcgcggat
     1381 cgcgccgcgg gcctccttcg tcgtccagga cagaccggtg tcgcccgggg cgtagcggta
     1441 ggacagggtg tatgtggcct tgcccagcac cggcacgtcg acctcgaacg cgacctcccg
     1501 ggctcggccg ccctcgccgc gttcacggat ctgcgccgtg cggacctccg cccactccgg
     1561 gtaggcctcg tagtcctcga ggacctccat cacgtcctcc aggggcgcgt cgatgacgat
     1621 cgatccctcc gcgtgctcca tctgcctcga tcctatttgg cggccggaat cctagccggg
     1681 gggatgtctc agcggctgtt gaccgacacg accgacaccg ccaggaggga gacggtcagc
     1741 atgatgaggg cgacggtcgc ccacaggagc gcgcgcgatg agacgaacgg cgacccgcct
     1801 ccggcgatcc gatacgcctc ggcggccgac ccgaggtaga gcaccatcgc ggagccgagc
     1861 aggagaacca ccacgccgaa gaccggcggg ctcgacaccc ccgacagcac gatcacgagc
     1921 gccagcccga acgagaggac gaacaaggtg ccacgagcga tgccgtcgag cgcccggccg
     1981 gccttcgcgt gtccgacccc ggggaacgtg agggaccaca agaaggcgtc gcgaggcgag
     2041 acggtggggg ccgcccgttc ctctcgcagg agctggccga aggaggtgcc gcagacgcta
     2101 cagaggtcga gggcgatgtc gttctgcgac ccgcacgtcg gacagggcca cgtgagcttc
     2161 gccggctcct cacgctcccc cggcctcccc tgctcttttc cttcctgagg ccgtgcgacc
     2221 acaggaacgg cggccgtggc cggctcgggg gtcggggccc gcagggagga gaagcattgt
     2281 ccacaccatt cggcatccgc gctcaccaac gcgttgcagt tcggacagcg gtgatcctcc
     2341 acagggcaag ggtaccgtcg gagccacgcc cgctcgggac gtgctccctc ccagccggga
     2401 ggacgacctg tcgcgtcagg cggcgatcgc ctcgcggagc catcccgcga ggcgttccgc
     2461 gacgcgagcc caggtgtgct cgtgttcgac acgggcccgg cccgctttcc cgagccgtcg
     2521 ggcgtacgcg ggatcctcca agagggtcgc gacggcgtcc gccaccccgc tcacctccgc
     2581 gccgttgacg agcagcccgg tcgctccgtg gacgagcgct tcgcgggagc cgccggagtc
     2641 gccgacgacc accggccgac cacatgcggc cgcttcgatg aagacgttcc cccacccctc
     2701 cacctcgagc ccggccaggc gggtccggca cggcatcgcg aagacgtctc cgacagcgta
     2761 gtagcggggt aggtcctcct cggacacctc acccacgaac gtgaccgagc gtgtgggcgc
     2821 gcgctccgcg agagcccgga gggtcgaccc gtagggaccg gagccgacga tcagcagtcc
     2881 cgcctcgggc acccgtcgct ggatcgagcg catcgcccgg atcaacacgt cctgcccctt
     2941 ccgtgggacc aaccggctca cgcagacgat cagcgggcgg tcgcccaggc cgtgccgttc
     3001 cctgatgtcc gcggtgggca gatccgggcg gaaccgctcg acgtcggccc ctggatggag
     3061 caccgagacc ggcacgtgct tcggcacggc ggttcggacc cggcgggaga tgaactcgct
     3121 gcacccagct actcgggacg cgcgcgccgt catccgcttc atgacggcat gggcgatcgg
     3181 caccgtcgag agccagtagt cgaaaccgtg cgcgaggacg accgagggcg tgcctcgcgc
     3241 cttgagctgc gacgccatca gggcgagcgg gaacgcgtcg ccgaacaaca ccacctccgc
     3301 gcccgatcga tcgacctcgg cgctcagcct gcggaccacg ccgggcgacg gccagacgaa
     3361 tcgccgacgc tcgcggacca cggtgaaggg gaccgacgcg tcgaagcgct ccgatccctc
     3421 gcacgacggg gcgatgaccg agatgcggtc gtggggaagc tcccggcaga gcgactcgag
     3481 cgttcgctgg atgccgccga ccctcggggg gaagtcgttc gtcacgacga gggtcctcgg
     3541 gatgtccacc tgcggatatc ctcccagagc aggctcgatg agggaggtgc ccaacggcaa
     3601 cgacgcaacc gaggcagcgg caacgacgtc ggaccttcgc ggtgctctcc gggtggggcg
     3661 tcaccgtcgt cgggggcacc gatgcccggt cgtggctgca cgacctggtc acggcggatg
     3721 tcgagggcct gcccgatcga acgtcccggc ggtgtctcct cctcactccc accggtcgga
     3781 tccgggccga cttccacatc gccgcgctgg acggctccta cctcctcctc caggcaccgg
     3841 gccagccaga ccgggtggat gcgatcctgc gaccgtacgt gttgtcgtcg gacgtcgagg
     3901 tgaccgatgt cggcgaccgc tcggaggtgg tggccgtcct cgacggctcc gccgtcgagg
     3961 gcggctccgg gtccgtcgtc ctgtccccgt ccgtcctcgg ccccgggtac gacgtcgtcg
     4021 tggcgtccgg ggacgcctcc gcacggctcc gggaacgcct ccgagccggt gggctgacag
     4081 aggcggatcc cggggacctc gaggcccgac ggatccgccg aggagagccg cgcatggggg
     4141 tcgatttcgg gccggacgcc ctccccgcgg aggcagggct ggaggacacg atcgacttct
     4201 cgaagggatg tttcctcgga caggagtccg tggcgaaggt ccgtaacctc ggacaccctc
     4261 cgcgggtctt gctccacgtc cggtccgggg ctccgatcgc ccggggcgcg cgagtcgtcg
     4321 cgggtgcgac cgtcgtcggg gaggtgacga gcgccgtgga agatcccgac ggtgtcgccg
     4381 cgatcgttcg ggtccggtgg gatgccgcaa cggagcaact cgccaccgac gccggaccgc
     4441 tttccctaca gagggttgag tagatacttc ggctcatttc gcatccgcca cctgggggtt
     4501 tgccaggacg cgactccgga ccccttcccc gccgtggtca ctccgtgcta ttccttcggt
     4561 cccggaccga ggggaccccc ggggggagga acggaacggt gcgaatcgat cgtctcccga
     4621 ccgtcgacgt cgacgtattg cccgacgatc cctcacctcc cgacgtctgg caggaacggt
     4681 ccgcgtgctt cgggatcgat cccgacgtct tcttcccgat ctcggaggag gaagcggggc
     4741 ccgcgctcgc gttctgcagc gcctgcacga tccggaccga gtgcctctcg tgggcgctca
     4801 agaacgggga gcgatacggc gtgtggggag gactcacgga gcagcagcgg cgtcgcctca
     4861 cccgccaggt cgcctgagct cccatcccgc ggtccatacc cgctccaggg cagtggatcg
     4921 ccccgagaac gggtatccat atgcctcgct gatccgccga ttcacttctc atggatgccg
     4981 tcgttcccgc accccaccgt cggcgttcgt tctcactgat ccggctcgtc gccggggccg
     5041 cggccgccac catgctcatc ctctccctcc cttcccccgc ggccgcggcc ccgacccctt
     5101 ccatcaccat ctccgacgtc accgtgaccg aaggcacggg ggccgctccc gtcacggcgt
     5161 cgttcacgat ccaggcggcg ccgcccccaa gggtgtgctg cgccctccag gtcggttggg
     5221 cgacggcgcc cgggtcggca tccgcgcccg gggacttcac gtcgtcttcc ggcaccgtct
     5281 cgctgacgaa gtcctcgagc tcgagggtca tcggggttcc catcgtcggg gacgccgcgg
     5341 atgagccgaa cgaagcgttc gtggtgaacc tgtccaacct ggtcggctct ccgggcacga
     5401 tcggtgatgc gcaaggggtc gcgacgatcg cggatgacga cggtcctccg agcctgtcgg
     5461 tgaacgacat ctcggcgacg gagggcaacg ccggcaccac gaccgccacg ttcacagtct
     5521 ccctctcggc ggcgagcggg aagacggtgg cgttcgactg gaccaccgcc gcgggaacgg
     5581 ccgccgcgga tgtggactac gtgaccgcca gcggcagtcg cacgatcgcc gccggcgcca
     5641 ccactgccac gatcgcgatc accgtgaacg gtgacgtcgt cgacgaggtc gacgagacct
     5701 tcacgctcgc tctgtcgaac cccgcgaacg ccgcgatcgc cgacgggtct gccctgggca
     5761 ccatctccga cgacgatgcg ccgcccgccc tctcggtgaa cgacgtctcc gtgaccgagg
     5821 gcaacgcggg aaccaccacc gccacgttca ccgtctccct ctcggcggcg agcgggaaga
     5881 cggtcaccgt cggatggtcg accgccgacg ccgatgccac ccagccggtc gactacaccg
     5941 ccgcgtcggg gacgctgacc ttcgtacctg gagacgcgag cgagacgatc gtcgtgagcg
     6001 tgaacggcga tctcgtcgcc gagctcgacg agatcctgga cgtcacgctc accgctccct
     6061 cgaacgccac gctcgcggat gctgccggga tcggcacgat cgtcgacgat gagctcctcc
     6121 ccgtgatcga catcgacgag cctatggccc tcgaagggtc gggttcgatc gtcttcacgg
     6181 cgtcgctctc gcaccagtcg gcctcgcccg tgacggtcga ctggagcacc gcggccggga
     6241 ccgcgacgaa cggcatcgac tacgtcgaga cgatcggcac cttggtgttc gccccgctcg
     6301 acatctccga gaccgtcgag atcacggtga acgaggacgg gaccttcgag cacggcgaga
     6361 cgatggcggt caacctctcg aacgcatccg gcgccccgat cggcaacgcc cagggcatcg
     6421 gaaccatcgc gaacgacgac gacgcgccgg gtctgtccgt cagcgacgtc tcggttctcg
     6481 aggggaacac cgggcagagg atgcttatct tctccgtctc cctcacgggt gacacggata
     6541 tcgatgccac gttcgacttc gccacggcag gtctcacggc gacccaggga accgaccacg
     6601 ccgacgccgc tggcaccctc acgatcgacg cgggcgacac cggcgccacg atcgacgtca
     6661 tcgtgaacgg cgacgcgacc tatgagagcg acgagaccct gtccgtgacg ctcagcgatc
     6721 ccgcgagcgc ggtcctcgtg gacagcgcag cgatcggcac gatccgcaac gacgacaagg
     6781 cgcccaccac cgtcaccgtc cgcatcgcgc gcgcaccgac gaggatcgtc gccaccggga
     6841 tcctggagca cgcgcatccg ggactccgga tcacggcaac cctgttccgg aaggcgcacg
     6901 gtcggttcct taaggtcacg gtgaagaccg ctcgcgtccg agaccttcgt gaccgcgacg
     6961 gcgacggcaa tccggacggc gcgtataccg cgaagttcct ccgcccgagg tggaaggggc
     7021 tctacaaggt cgcggtccgc ttcaagggct ccgcggccta caagccctgc actcggtcga
     7081 agatcttccg cctctccgcg acgtagcccg agccgatgta ggccccgcgc tccccaggat
     7141 gggcaaaagg gcacaccgga gagccctgat ccttgaccat cgaacatctg ttcgatacgg
     7201 tgtgcctccc cgtcggctcc accgatcgga ggcgcccgtg ctctcggtac ccatccagac
     7261 cacactcgcg gagggcgtgc cactcaagga ggtcgacttc gtggtggtgg acctcgagac
     7321 gacgggtggc tcccctcggg actcgaggat caccgagatc ggcgcggtcc gcgtgctcgg
     7381 cggagagcgc atcggcagct tccaggcact cgtgaacccg ggggaagcca tccccccgtt
     7441 catctcgcac ctgacgggca tcgacgatct cctcgtccgc caggagcccc cgatcgaagc
     7501 ggtgcttccc gcgttcctcg agttcgcccg gggggcgatc ttcgtggccc acaacgcgcg
     7561 attcgacttc tcgttcctga acgagaacct cggtcggctc gagtacccct tgctggaggg
     7621 accgcccgtg tgcacggcga agctcgcccg gcgcgtggtg tggccggagg ttcccaacgt
     7681 ccgactgcga acgctcgccc ggtacttccg cacccgggta cagccgatcc accgggcgct
     7741 cgacgacgcc atggcgtgcg ccgaagtgct cgatcagctc ctcgacctcg gcgcccgcct
     7801 ggggatcctg acgctcggcg atctccgcca ggcggtcacg gcacgcgggc gtccccacta
     7861 cggcaagatc cgcctcgcgg acgacctccc tcgcgcggcc ggcgtctacc gcttcgtcgg
     7921 tcgggacgat cgcgtcctct acgtcggcaa ggcacgggac ctccgggcgc gggtgaagtc
     7981 ctacttctac ggcgacgggc gcaagaaggt ggagaacctc ttgggtgagg tccaccgcgt
     8041 ggatggggag gaaacgccgg ggggcgagct cgaggctctg gtcctcgagg cgaggctgat
     8101 ccggcgccac gagcccaagt acaaccgacg cggtacgacg tggcgccggt tcgcctacct
     8161 gaagctcgat ccgaccgagg ccttcccccg gctcaaggtc gtccatcgag ccggggccga
     8221 cgacggctgc gcgtacctcg gacccttctc gggatcgggg gctgcccggc tggcgaagga
     8281 ggcgctcgag gagatcgtcc cgctgcgtcg ttgcacccgc tcgatgggcg cacgcacccg
     8341 gttcgcccct tgcgccctcg ccggtatcgg ccggtgcacc tccccgtgcg acggcgggat
     8401 cgaccccgag cgctacggag agctcgtccg ggatctcctc tcctccctcg agtccccgga
     8461 cgggctcctc ggagcgctcg aagcacggat gcgccggctg gcggacgccg aacgattcga
     8521 agaggccgcc tccgtccgcg atcgacttcg tgccctcgcc tgggcgttgc atcgctcgcg
     8581 ccaggacgcc tggctcgtcg gcgccggtca cctcgagctc gggacggaag gcgagcggcg
     8641 actggccttc gtggggggtg cgcttgcgcg ggccaacgac acggacgtgg agcccatccc
     8701 agagccctgc ccgcgtgagc gcgccgacga gctgtcggcg gtgcgttcct ggcttgccag
     8761 gaaccccgtc cgtctgatcc ggtgcgacgt ccccctctcg gagcccgtcg acggtggcgc
     8821 ccgtatcgcg aagatagtcc gttggacgaa taaggacccc gagcgacctc gcgcgagccg
     8881 gcggtaggct cgccctgtcg tgggccctgc cgtcgagctc ctcgaacgcg agatgtcgga
     8941 agccttccac gacatgcggc aacgcctgca cggtctggcc gacgaggagt tctcctggga
     9001 gccgatctcc ggctcgtgga cgatcttccg aaccgaggac ggccgctggg accaccacta
     9061 cgaggaaccc gatccagtcc cggcaccgtt cacgacgatc gcctggcgtc tgacccacct
     9121 cgcgatgtgc aaggtgatgt accacgagca cgccttcggc ccgggcgccc tcacctggcg
     9181 caccatcgag actcccggaa ccgtggcgga cgcgatcgcg atgctcgacg cgggccacgc
     9241 cctcctgacc caggatctgg ccgaggtcga cgaggccgat ctggagcgac cggtgcggac
     9301 caactggggc gagctctggc cggtctggcg gatcttctgg acgatgatcc agcacgacgc
     9361 ccaccacggc ggcgagatcg gagcgctccg cgacctctac gccgggaccc gcgccaaggg
     9421 ctgatccctc cacgatctga tcgaccgagg tcctgaagcg aggcccggat acactggccg
     9481 ccatggagca cgcggtgatc ctcgagggcg cccggacccc gatcggcaag ttcctgggct
     9541 ccttcgcagc caccccggtg gtcgagctgg gggcgaccgc ctcacgcgag gccctgcgtc
     9601 gagccggtgt gtcgggggat cagatcgatc agacgatcat gggtcacgcg cgtcaagcgg
     9661 gcaacgggcc gaacaccgga cggcaggtga gcatccacgc cggcgtcccg gaggaggtct
     9721 ccgcctacaa cgtcaacatc gcgtgtggct cgggcatgaa ggccgtgcaa ctcggcgcgc
     9781 agcagatcgc cctgggcgac tccgaggtcg tcctggccgg cggcatggag aacatgagcc
     9841 gcgtcccgtt cctgctcgac cgcatgcgga cggggtaccg gatgggaaac gccaccgtgc
     9901 acgacgcgat gtaccgggac ggtctccttg atcctctctg cggtctgctg atgggggaaa
     9961 cggcggagaa cctcgtcgat cgctacgggc tctcgcgcga ggagcaggac gggttcgccc
    10021 tcgagtccca ggcgaaggcg gcccagggaa ctgatgcccg cgcggccgag atgttcccgc
    10081 tggaggcgaa cgacccttcg gggaagggca cgctcacgat cgctgaggac gagcatccgc
    10141 gccccggaac cacggccgac gggctcgcga ggctctcgcc ggtgttccgc gagggcggct
    10201 cggtgaccgc cggcaacgcg gccggcatca cggacggagc cgcagcgatg atcctgatgt
    10261 cggcctcacg cgcccgcgcg gagggacgag agcccctcgc ccggatcgcg gggatgagct
    10321 gggcgggcgt cccgccggag atcatgggca tcggacccgt gccggccacg aagaaggtcc
    10381 tcgagaagac cggtctctcc ctggccgaca tcgacctgat cgagatcaac gaggcgttcg
    10441 cggcacaggt gctcgcgtgc gagcgtgagc tgaagttcga ccgggaccgg ttgaacctcc
    10501 tcggtggcgg catctcgctc ggccatccga tcgggatgag cggc
//
LOCUS       DASUYD010000257         7442 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1751452, whole genome shotgun sequence.
ACCESSION   DASUYD010000257 DASUYD010000000
VERSION     DASUYD010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 7442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7442
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1751452"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF195_13440"
     CDS             <1..382
                     /locus_tag="VF195_13440"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HEX6581855.1"
                     /translation="LGVGSLADVQFAVELSLMRFGSAEPEVRSRRTLEAIEKLAAARH
                     LEDQVARDLAEAFVFFSDVKNVLEMDRRVHAEALPAAPGEQTALARRLGFEEYPRQAF
                     LERYLHVTRRCRRAMERVFAQALA"
     gene            407..766
                     /gene="mscL"
                     /locus_tag="VF195_13445"
     CDS             407..766
                     /gene="mscL"
                     /locus_tag="VF195_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003571482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEX6581856.1"
                     /translation="MIKEFKEFISKGNLVEIAIGLILALAFAGLVTSFTENLINPIIG
                     AIFGQPNFDSLVIEMGDAELRYGAFLTTLLNFVIVAFVLFLVVKAYNRAFPKKEAAAG
                     PTEIELLTEIRDALRQR"
     gene            814..1098
                     /locus_tag="VF195_13450"
     CDS             814..1098
                     /locus_tag="VF195_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581857.1"
                     /translation="MQIDATSDAKKLIRARGGMLFVWMEAGMRGLRFLRASTEPPPDA
                     LEWDRTEAPEGFLVFTPPRMRKPRTLGLEVRGFLTKLRINPLWNGCAFVV"
     gene            1832..3100
                     /gene="dcm"
                     /locus_tag="VF195_13455"
     CDS             1832..3100
                     /gene="dcm"
                     /locus_tag="VF195_13455"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013410469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEX6581858.1"
                     /translation="MAARKGRARPGSAGRVVELFAGVGGFRLGLEPNGWETVWASQWE
                     PSTKTQHAADCYRHHFEPDVLSNEDINAVVEQMDRDGWDCFPDHDLLVGGFPCQDYSV
                     AKARSQAEGIVGKKGVLWWAIKRIVEKKRPRLIFLENVDRLLKSPASQRGRDFAIILA
                     CLSDLGYLVEWRVVNAADYGFPQRRRRVFMVAHRVGDELEPDWSGPISMMARDGALAR
                     GFPIEPPIATDVDAPTLKLEGDLAELTQAFGVGNGQTPFHAAGIMWRRAVWTGPVATK
                     YSGPRAVLMDVLEAVHNVPESFFIREEQRERWAYLKGPKNIPRIAKNGHEYLYSEGGI
                     AFPDPTDQPSRTVLTGEGGVTPSRFKHVIDAGDGRLRRLTPLELERLNGFPDDWTSTG
                     MPDGRRAFMMGNALVVGLVSRVGKALAEIG"
     gene            complement(3097..3732)
                     /locus_tag="VF195_13460"
     CDS             complement(3097..3732)
                     /locus_tag="VF195_13460"
                     /inference="COORDINATES: protein motif:HMM:NF020757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HEX6581859.1"
                     /translation="MEFVTFNFRHAREILEDRDSWGELAQATQSIGRADILRTHQAFA
                     AEGKRLPAGGQTSINRLFRERLAPIGWVGEPRLFPTDQEALRKWKMDFIKDRIGVEVS
                     FNHAEAVPWTFTRLNIAGESDKVIPDHRIDVGVAFFATETLKHWARMDSAVGTFELAK
                     AWLEMMKPIMPIPVLVVGLSAAGWEETDAFRGTRAPRVRPRLAEIPPLPSD"
     gene            3757..4095
                     /locus_tag="VF195_13465"
     CDS             3757..4095
                     /locus_tag="VF195_13465"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX6581860.1"
                     /translation="MERNSWTDVAGIDELVDARGIRVGIASDEILLVKSSESVFAIGN
                     ECTHQGAGLDKGVVKIAGSVKTVTCPAHGSTFNLETGKVMRPPASKPVPVYDVKIEGG
                     RVFVRPREES"
     gene            complement(4092..5006)
                     /locus_tag="VF195_13470"
     CDS             complement(4092..5006)
                     /locus_tag="VF195_13470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEX6581861.1"
                     /translation="MSLVTDHVTRSIPIEEPYDLAGTLFPLRRGTGDPTMRIEERVAW
                     RAARTPDGPATLLLEQMNGAVCATAWGTGAAWALDHAPGLIGAEDDISAFVAHDDVMA
                     RVWKEHRSVRLTRALDVVRSLVAAILEQKVVGMEARRAWRRMTLSLSEPAPGDAGLML
                     PPDPTTVAEVPYYRFHPWGVERRRAEVIRAVCARATSLEALADVPFDEARRRLEAMRG
                     IGPWTAAEVMRLSFGDPDAVSVGDYHLPDVVGWALAREERGTDERMLELLEPYRGQRG
                     RVQVLLERSHIAPPAYGPRMEARSIEKL"
     gene            complement(5003..6454)
                     /locus_tag="VF195_13475"
     CDS             complement(5003..6454)
                     /locus_tag="VF195_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6581862.1"
                     /translation="MARSPRWAWWAAVVGPLLVGVAGAALALQAFAKTTVDAGPFQIQ
                     VEADFGRSVTDISLPPLGRVRADTHGAPLHLRASLREVDVDQLQEGLRRGVDSVAAEV
                     ERNTLDAAGRFAAWVVLVGIAGAAALGLLAFRNRWLLVLRATVSGLAAVLLSVGVTAL
                     GFDAAAFQAPSYTGSLRLVPQLFGPVEGAIERVGYFREELRRVVAGAARAYAAIESNP
                     LGRGDEIRVLHISDIHLSTLGYDFAQELARSFDVDLVMDTGDTTSFGAPDEEFILSEI
                     PSFGLPSVWVRGNHDSAAFQEALDRVEGTVVLDGDTEEVDGFTIYGLGHPYFNEERGT
                     PVGDEDVIELVAGAADQVLADVTTLPEPPDIVLVHDDRMAAEIAGLVPLVLSGHFHDA
                     RDEVRDGTVFLRVGTTGGAGPTGFTAEGDVPFSAEVLYFQPDEEGRMDLVAWDVVTQF
                     PETGSLQIARHLASDVGLSPSPSVLTPTSPSPS"
     gene            complement(6521..7012)
                     /locus_tag="VF195_13480"
     CDS             complement(6521..7012)
                     /locus_tag="VF195_13480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX6581863.1"
                     /translation="MTKISPFLWYDDKAEEAAEFYVKVFREWSGATGGTEIHSVARYP
                     EGSPAPGAAMTVSFQLEGQEFTALNGGPDHRFSDAISFVVSCASQGEVDELWSALTDG
                     GEESMCGWLKDRYGLSWQIIPTELYDLLGDPDPGRAQRATQAMLQMQKIDIAEIRRAA
                     DAA"
     gene            complement(7246..>7442)
                     /locus_tag="VF195_13485"
     CDS             complement(7246..>7442)
                     /locus_tag="VF195_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEX6581864.1"
                     /translation="SVKQVPGSLEEVLDTLEGDHQWLLEGGVFTQDVIDTWIEYKRAH
                     EVDAIRLRPHPHEFHLYYDV"
BASE COUNT         1206 a         2416 c         2579 g         1241 t
ORIGIN      
        1 gctcggcgtc ggatcgctcg ccgacgtgca gttcgcggtc gagctgtcgc tgatgcggtt
       61 cggcagcgcc gagcccgagg tgcgctcacg ccgcacgctc gaggcgatcg agaagctcgc
      121 cgccgcgcgc cacctggagg accaggtcgc gcgcgatctc gcggaggcgt tcgtgttctt
      181 ttcggacgtg aagaacgtgc tcgagatgga ccgtcgcgtc cacgccgagg cgctcccggc
      241 ggcgccgggc gaacagacgg cgctcgcacg taggctcggc ttcgaggagt acccacggca
      301 ggcgttcctg gagcgctacc tgcacgtgac ccggaggtgt cgccgggcca tggagcgggt
      361 gttcgcccag gcgctcgcct agagagctaa ggaaggagaa ggagcgatga tcaaggagtt
      421 caaggagttc atctcgaagg ggaacctggt cgagatcgcc atcgggctga tcctcgcgct
      481 ggccttcgcg ggcttggtga cctcgttcac agagaacctg atcaacccga tcatcggcgc
      541 catcttcggc cagccgaact tcgacagcct ggtgatcgag atgggggacg ccgagctccg
      601 ctacggcgcg ttcctcacca cgttgctcaa cttcgtgatc gtcgccttcg tgctgttcct
      661 ggtggtcaag gcctacaacc gggcgttccc caagaaggag gcggcggccg gaccgaccga
      721 gatcgagctg ctcacggaga tccgcgacgc tctccggcag cgctgatctc gcccgggtgt
      781 gaacttttcg gccccgggta ccgatacagg gatatgcaga tcgacgcgac ctccgacgcg
      841 aagaagctga tccgcgcgcg cggcgggatg ctgttcgtct ggatggaggc cgggatgcgg
      901 ggccttcggt tccttcgggc ctcgacggag cctccgccgg atgcgctgga atgggaccgc
      961 accgaggccc ccgagggatt cctcgtcttc actccgccga ggatgcggaa gccaaggacg
     1021 ctcggcctcg aggtgcgagg attcctgacg aagctccgca tcaaccccct ctggaacggc
     1081 tgcgccttcg tcgtctagcc gtaccctctc atccgtggat gtccgctggt tccgtcggcg
     1141 gctcctggcg tggggtcggg agcatcgtcg ctcattcccc tggcgcgaga cggacgaccc
     1201 ttggcgagtt ctggtcgcgg aggttctgct gcagcgatca cgtgggaaga ctgtcgcgaa
     1261 ggtctacgag gagctgtttc gccgctggcc agacgcactc tcgatgagta gagctcgtga
     1321 cggttcgatt gctgcggtga tccgtcccct cggtttggtg cgacgagcgg acacgctccg
     1381 ggcgatggcg aaggaggtga ctcgtctcgg cggggttccc gcggaactgg ctgccctcgt
     1441 gaatctcccc ggcgtgggtc ggtacgcagc caacgccact ctcgtcgtcg ccttcaggaa
     1501 acgggccccg gttgtcgacg gggtgagcgc gcgcgtgtac cggcggtatc tgggcctacc
     1561 gtcagagctg ccagccacct ccgatccttc gctctgggaa gaggtcgcga gagcaactcc
     1621 tccaaacgct tctcgagagt ggaactgggc tgtcctagat ctggccgccg atgtctgtct
     1681 tcccgccaat ccatgttgcg gcggatgccc tctggcggcg cggtgtgcct tcgctcgggc
     1741 ggcttgatcc ttgtcggccg accgtgctag catccggcga gcggaatcac agaggtgtga
     1801 ttcactccgc gcgactcgcc agggaggtcc ggtggcggca aggaaggggc gcgcgcgacc
     1861 ggggagcgcg ggaagggtcg tcgagctctt cgcaggcgtg ggcgggttca ggctcgggct
     1921 cgaaccgaat ggctgggaga cggtgtgggc cagccagtgg gagccctcca ccaagactca
     1981 gcatgcggct gactgctata ggcaccactt cgagccggat gtcctgagca acgaggacat
     2041 caacgcggtc gtggagcaga tggaccgtga cggctgggat tgctttcccg atcacgacct
     2101 cctcgtgggt ggcttcccat gccaggacta ctcggtggcg aaggctcgca gccaggcgga
     2161 aggaatcgtc ggcaagaagg gggtcctgtg gtgggcgatc aagcggatcg tcgagaagaa
     2221 gcgtccacgc ctgatattcc tcgagaacgt cgaccggcta ctgaagtcgc ccgcctcaca
     2281 acgggggcgt gactttgcga tcatccttgc ttgtctgtcg gatctcggct atctggtcga
     2341 gtggcgcgtc gtgaacgccg ccgactatgg attcccccaa cgccgtcgcc gtgtgttcat
     2401 ggtcgcgcac cgcgtcggag atgagcttga gccagattgg agcggtccga tctcaatgat
     2461 ggcgcgcgac ggcgctctcg cccgtggttt tccgatcgag ccgccaatcg ctacggacgt
     2521 cgacgcccca acgctgaagc tggagggaga tttggctgaa cttacccagg cattcggggt
     2581 tggcaacggt cagactccct ttcacgcagc gggaatcatg tggcgaaggg cggtttggac
     2641 aggcccggtc gcaaccaagt actcgggccc gagggcggta ctgatggatg tcctagaggc
     2701 ggtacacaac gttcccgaat ccttcttcat cagggaggag cagcgggaac gatgggcgta
     2761 tctcaagggt ccgaagaata tcccccgcat cgcgaagaac ggacacgagt acctctactc
     2821 ggaaggtggg atcgcgtttc ccgatccgac cgatcagcca tcgcgcacgg tcctcacagg
     2881 tgagggtggt gtcactcctt cccggttcaa gcacgtgatc gatgcgggag atgggcgact
     2941 ccgtcgattg acgcccctgg agctcgaacg cctcaacggg ttcccggacg actggactag
     3001 cacggggatg cccgatggcc gccgcgcatt catgatgggg aacgccctgg ttgttgggct
     3061 ggtcagccga gtcgggaaag ctctggccga aatcggctag tcgcttggga gcggagggat
     3121 ttccgcgagt cggggtcgaa cccgaggcgc gcgtgtgcca cggaacgcat cagtctcctc
     3181 ccatccagcc gcgcttaacc cgacgaccag gacggggatg ggcatgatgg gtttcatcat
     3241 ctcgagccat gccttggcaa gttcgaatgt gcccacggcg ctatccatcc gcgcccaatg
     3301 tttcagagtt tcggtcgcga agaatgcaac cccaacgtcg atccggtgat ctggaatcac
     3361 cttgtcggat tcaccagcga tgttcaggcg cgtgaatgtc cacggcacag cctcggcatg
     3421 gttgaaggag acttcgacgc cgatccgatc cttgatgaag tccatcttcc acttccgcaa
     3481 agcctcctgg tccgtgggga acaggcgagg ctccccgacc cacccgatgg gagccaaacg
     3541 ttcgcggaaa agccggttta tcgacgtttg cccgccagca ggcaagcgct tcccttccgc
     3601 tgcgaaggct tgatgcgttc gaaggatgtc ggcccggccg atggactggg tcgcttgcgc
     3661 cagctcgccc caggagtcac ggtcctcgag gatctcgcgg gcgtgacgga agttgaaggt
     3721 gacgaactcc acggcgaagc atggtaggac ctaactgtgg agaggaacag ctggaccgat
     3781 gtcgcgggaa tcgacgagct agtcgatgcg cgggggattc gcgtcgggat tgctagcgac
     3841 gagatcctgc tcgtcaagtc atccgagagt gtcttcgcga tcgggaacga gtgcacacat
     3901 cagggcgccg gtctcgacaa gggggtcgtg aagatcgcgg ggtcggtcaa gaccgtgaca
     3961 tgcccggctc acgggagcac gttcaatctc gagaccggga aggtgatgag gccgccggcg
     4021 tcgaaaccgg ttcccgtgta cgacgtgaag atcgaaggcg gccgggtctt cgttcggccg
     4081 cgcgaggaga gctagagctt ctcgatcgag cgggcttcca tcctcggccc gtacgcaggc
     4141 ggggcgatgt ggcttcgctc gaggagcact tgcactcgcc ctcgctgacc gcggtagggc
     4201 tccaggagct ccagcatccg ctcgtcggtg ccacgctcct cgcgcgcgag tgcccatccg
     4261 acgacgtcgg ggaggtgata gtcgccgacg gacacggcgt ccgggtcgcc gaacgagagg
     4321 cgcatcactt ccgccgctgt ccatggtccg atgccgcgca tcgcttccag ccggcgccgg
     4381 gcctcgtcga acggcacgtc ggcgagggcc tcgagcgacg tcgcccgcgc acagacggct
     4441 cggatcacct ccgcgcggcg gcgctccacc ccccacgggt ggaaccggta gtacggcacc
     4501 tcggccaccg tcgtgggatc cggtggcagc atgagtccgg catctcccgg ggccggttcg
     4561 ctcagggaga gcgtcatccg acgccacgcg cggcgggcct ccatccccac gaccttctgc
     4621 tccaggatcg cggcgacgag cgatcgcacc acgtcgagcg cccgcgtgag ccggaccgac
     4681 cgatgctcct tccacaccct cgccatcacg tcgtcgtgcg cgacgaacgc cgagatgtcg
     4741 tcctccgcac cgatcagccc gggcgcgtgg tcgagggccc acgcggcgcc cgtgccccag
     4801 gccgtcgcgc acacggcccc gttcatctgt tcgaggagca acgtcgccgg gccgtccggg
     4861 gtccgcgcgg cacgccaggc gactctctcc tcgatccgca tcgtgggatc gcccgttccg
     4921 cgccggagcg ggaacagcgt acccgcgagg tcgtagggct cctcgatggg gatcgagcgg
     4981 gtcacgtggt cggtcacgag gctcacgatg ggctcggtga ggtcggggtg agcaccgacg
     5041 ggctcggtga gagtcccacg tccgacgcca ggtggcgggc gatctggagg ctcccggtct
     5101 ccgggaactg cgtcacgacg tcccacgcga ccaggtccat gcgcccctcc tcgtcgggct
     5161 ggaagtagag cacctcggcg gagaagggga cgtcgccctc ggcggtgaag cccgtggggc
     5221 cggcgccgcc ggtcgtcccc acgcggagga acacggtgcc gtcgcgcacc tcgtcgcgcg
     5281 cgtcgtggaa atggcccgag agcacgagcg ggacgagccc cgcgatctcc gccgccatcc
     5341 ggtcgtcgtg gacgaggacg atgtcgggcg gctccggcag ggtggtcacg tcggcgagga
     5401 cctgatcggc cgcgccggcg accagttcga tgacgtcctc gtctccgacg ggggtgcccc
     5461 gttcctcgtt gaaatacggg tgaccgagcc cgtagatcgt gaagccgtcg acctcttccg
     5521 tgtcaccgtc gagcacgacg gtcccttcca cccggtcgag ggcctcctgg aaggcagccg
     5581 agtcgtggtt gccgcggacc cagacagagg ggagcccgaa cgaggggatc tcggaaagga
     5641 tgaactcctc atcgggtgcc ccgaaagaag tggtgtcgcc ggtgtccatc acgaggtcga
     5701 cgtcgaagct ccgggcgagc tcctgcgcga agtcgtagcc gagtgtggag agatggatgt
     5761 cggagatgtg gaggacgcgg atctcgtcac cccggccgag cgggttcgat tcgatcgcgg
     5821 cgtacgcgcg ggcggcgccg gccaccacgc ggcggagctc ctcgcggaag tagcccacgc
     5881 gctcgatcgc gccctcgacg ggaccgaaca gctgcggcac cagccggagg gaccccgtgt
     5941 agctcggtgc ctggaaggcc gccgcgtcga agcccagtgc cgtgacgccg acggagagca
     6001 gaacggccgc gagcccggaa acggtggcgc gaagcacgag gagccagcgg ttccggaacg
     6061 cgagcagtcc gagcgccgcc gctcccgcga tgcccacgag caccacccag gcggcgaagc
     6121 gacccgcggc gtcgagcgtg ttccgctcga cctcagcggc caccgagtcg accccacgcc
     6181 ggagtccttc ctggagctgg tcgacgtcga cctcgcggag cgacgcgcgg agatggagcg
     6241 gcgcgccgtg ggtgtcggcg cggacccggc cgagcggggg gagcgagatg tccgtgacgc
     6301 tccggccgaa gtccgcctcg acctggatct ggaacggccc ggcgtcgacc gtggtcttcg
     6361 cgaaggcctg gagggcgagg gcggcgcccg ccacgcccac cagcaggggc ccgacgaccg
     6421 ccgcccacca ggcccagcgt ggcgagcgtg ccatggcggg caccgtaaca gcgacccgcc
     6481 ggcggctgtc cccgccggcg ggtcgctcgg atcgccgttc ctacgctgcg tcagcggctc
     6541 gacggatctc cgcgatgtcg atcttctgca tctggagcat cgcctgggtc gctcgctgtg
     6601 ctcgcccggg gtcggggtcg ccgaggaggt catagagctc cgtggggatg atctgccagg
     6661 acaggccgta ccggtccttg agccagccac acatgctctc ctcgcccccg tcggtgagcg
     6721 cggaccagag ctcgtccacc tctccctgcg acgcgcagct cacgacgaag gagatcgcgt
     6781 ccgagaaccg gtggtccggg cccccgttca gggccgtgaa ctcctgtccc tcgagctgga
     6841 aggacacggt catcgcggcc ccgggcgccg gggatccctc cggatagcgg gcgacactgt
     6901 ggatctccgt cccaccggtg gcgccgctcc actcgcggaa gaccttgacg tagaactcgg
     6961 cggcctcctc ggccttgtcg tcgtaccaca ggaacggact gatcttcgtc atctctggcc
     7021 tcctcctctt cggcccctcg gtggggcgtt caccatctgg tcggagcggt ctcgccgttc
     7081 tcgacatcac ccggaaggga accgtagccg ggtttgctcg tgcgatcggt ggggaatctc
     7141 actcgcaggc ttcgggggcg agaaccgcac tcggagccca aggtgaacag agcgggcccc
     7201 ttggggaagc gggcccgctc gttcgcgtcc gggggcggtt cggccctaga cgtcgtagta
     7261 caggtggaac tcgtgagggt gcggccgcag ccggatcgcg tcgacctcgt gggcccgctt
     7321 gtactcgatc caggtgtcga tcacgtcctg ggtgaagacg ccgccttcga gcagccactg
     7381 gtggtcgccc tcgagcgtgt cgagcacctc ctcgagggag cccgggacct gcttcaccga
     7441 tg
//
LOCUS       DASUYD010000258        10543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1090717, whole genome shotgun sequence.
ACCESSION   DASUYD010000258 DASUYD010000000
VERSION     DASUYD010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1090717"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF195_13490"
     CDS             <1..322
                     /locus_tag="VF195_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581865.1"
                     /translation="DRKWAQIRLEGQAFGDVPLNVELKLEVWDSPNSAGIVIDAIRCA
                     KLALDRGQAGPLIGPSAYFMKSPPVQFSDEAAREMVEQFILGNGASKDADWTAFFDIE
                     PAEA"
     gene            641..1225
                     /locus_tag="VF195_13495"
     CDS             641..1225
                     /locus_tag="VF195_13495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581866.1"
                     /translation="MHSIARSSHAITLLAAAVLGWWFSSAVADASVSIRPQFFVYDAS
                     PEQVQMARWAAGRFEIAKLRVPEVEVHFHDDLSDCDGHLGYAVDGRVDLCTSVVDAPT
                     RRALLHELGHVWLDENLAPSRQAAFLRLRDLSAWNSSSDPWNLRGYEHGAEVMAWALG
                     ERILTPSIPDNEPEKLGPAFRFLTGGELPDAPDP"
     gene            1484..3349
                     /locus_tag="VF195_13500"
     CDS             1484..3349
                     /locus_tag="VF195_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX6581867.1"
                     /translation="MTREPAPTEVATYGIESLLGRGGMGEVYLAEDRRLHRKVALKVL
                     SAEFATNASFRERFLAESELAASIDHPNIVPIYEADEAGGYLYIAMRYVEGTDLKARL
                     RQGPLPVDQAVDLLSQVAGALDLAHERGLVHRDVKPSNVLVAPGAGHDGQDHAYLADF
                     GLTTRLADGGSLADDGQLMGTVDYVAPEQITGGEVDGRADVYSLGCLLVECLTGEPPF
                     QGSSDIAVLFAHLESTPPRPSERRPDLPEGLDRVVRTALAKDPNERYQTCLELVTAAR
                     AALGIAEPAHRRSRVAAVVGVAAIALVATTIWLTIGAGDEPRDRLLRIDPSTNEVVDS
                     VDVGDRASSVDIGDGQVWVTSQAGRALWRVDPRTLETRETPVEGTPMDVVERNGLAVV
                     VSGPLEVAVHRIDAATGALLETIPIPGAEGAATAVAEGDEGIWVVGCGLGGGNVGRVS
                     ETAFSGNSLLDRVEIFSKNPNFVFGHEPDFPAYTDVAVGEGGVWMTLDSGPALRRADP
                     TGQRPVEVIELPFFPRSVTVGAGSIWITGLIDDVVARFDPETREVTMTVPLGRGTAGV
                     AVGAGSVWVVSSIDGTVTRLDPRTGEIQATIEVDGAPEDVVIGAGGVWVTSDPDA"
     gene            3342..4667
                     /locus_tag="VF195_13505"
     CDS             3342..4667
                     /locus_tag="VF195_13505"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX6581868.1"
                     /translation="MPRRILALTCSLVVLGLTSCDRPQPDAIRIGILADCDTFGAAFY
                     DVALAGAELPLLRRGGSLDPSHLADGVEGVSVGRHPIELAFGCAGDPVGTATETRRLV
                     EVEGVEVVIGPNVSPNFTLIDYARRHPARAFVLATMEQLGDAPPNAFRFTASSVQMGA
                     GLGSYAFRELGWRRASSIAVPDFWQWGIYAGFLAEFCSLGGKIPDRYWLESTDEEITA
                     GLAEIPIETGDGFFLSVKPADAATFLRRYSADHPSLSQRVILGAYSSYPISLDPEVVK
                     GAGDSLMGVVTATFVPLDRSQDDWNAFVSEFGSSFPALADAADSTYHLFDIDYVNAME
                     AVMQALEAVDGDLSHRASGFQFALSDVRLDAPNGTISLDERRQAILPIYLSRVERADD
                     GDLVLRTFRKIEAVDQSYGSLLNADVPVPDRTYPPCVEGDPPPWATAVS"
     gene            complement(4660..6282)
                     /locus_tag="VF195_13510"
     CDS             complement(4660..6282)
                     /locus_tag="VF195_13510"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HEX6581869.1"
                     /translation="MAVDYRVLGPLEAFDDGRQLSLGGPKQRAMLASLLLRATEVVPV
                     GRLIDDLWGDDPPESAANIIQGYVSGLRQVIGREAIATRGRGYVLHVEPSNLDLRRFE
                     RLVDEGGGALANDRPLEAVEAFSKALRLWRGPPFADISDEPFVVVAAGRLDELRLAAL
                     EKRIDADLACGRHGEVAAELAALVEEYPFRERFRAQHMLALYRSERQTDALASYQAAR
                     AKLAEELGIDPSPALQSLEKSILDHDSSLDLSVSGGERLPELSEPRRVVLVAVLDGSR
                     LPTMLDLGAALVRDGRFELVIARLVRDQGELVAETARLNDGVAALEKRGVTARAAAFT
                     SQGPGEDLVRLSSEQDVVLLIVDPPQALVRDGLPDTDVARVLSHAPCDVALLVGAGGP
                     AAGPVVVPFGGTEHDWAAIELGAWYARARGLPLRLIGADAFPDAGKRDASMLLFHASV
                     AIQRAVGIATDPVLAAPGGEGMLSAAEGAGVLVVGLSDRWQREGLGPLRLALVRGAAQ
                     PTCVVRRGLRPGGLAPRESLTRFTWSVGPPVS"
     gene            6430..6651
                     /locus_tag="VF195_13515"
     CDS             6430..6651
                     /locus_tag="VF195_13515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581870.1"
                     /translation="MLGAGVPPVGIPTHPPADGTSVASVVLMIVFVTAVALAIAIQVR
                     AGRRNATGDRDAGPDEEVEPRQVEELLAR"
     gene            7547..7783
                     /locus_tag="VF195_13520"
     CDS             7547..7783
                     /locus_tag="VF195_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581871.1"
                     /translation="MDDEGNIYVADTYNRRVRKIDTDGIITTVAGNGTQAFSGDGGPA
                     TSAGLAKISGVAIGPEGALYIADSAHDRVRRVLL"
     gene            8006..9334
                     /locus_tag="VF195_13525"
     CDS             8006..9334
                     /locus_tag="VF195_13525"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX6581872.1"
                     /translation="MVSEDPYTNPGTYHRTQVEPDSFAFGSTVVSVFQSGRSYRCGAS
                     NIGWSVSRDAGTTWTDGFLPRTTPHSAPQGPWKRATDPVVAYDAAHDTWLAQVLGIPS
                     CPFAGGDVIVSRSTDNGATFGEPVIIKRQHRTQGFDKNWIGCDNFPDSPFYGTCYAAW
                     DDYRRYGFLYGEGIWHPLIHAYRSTDGGLTWSKAALPEDPCGVVNQIVVQPSGKAIIQ
                     SNDCSPRHQVFVSEDGGRTYKGPFRISTSRVRGAAGMLRSPYFMSSDVDASGRIYTVW
                     RDCTFRSFEGGDCTHNDLVMSTSNDGRHWSDVVRIPIDPRSSSVDHFLPALAVDPRTS
                     GATAHVAIAYYFYPQQRCDATTCDLSVGFTSSVDGGSTWSVQELAGPFKNTWFPLTTS
                     GYRVGDYIGISFVDGKAIPVFIVGSEGECELGDVTSCNVWTASATISLAS"
     gene            9559..9960
                     /locus_tag="VF195_13530"
     CDS             9559..9960
                     /locus_tag="VF195_13530"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005464581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX6581873.1"
                     /translation="MQTSRVNPAAVHPPVGTYSHSVRVGTADGTWICVSGQVAFDAEG
                     TLVGRGDMHAQTRQVFENLRAILAANDASFADVVKIGTYLTTLDDLAGMREIRSEYLT
                     SEPPASTAVQVAGLVVPDALIEIELVAVVPT"
     gene            9957..>10543
                     /locus_tag="VF195_13535"
     CDS             9957..>10543
                     /locus_tag="VF195_13535"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6581874.1"
                     /translation="MTAVAVLGLGAMGHAVADRLLGARHDIRVWNRTPGRDDDLVASG
                     AGRSTTPSDAVRDAEVVITMVTDPPALEEVLFGPNGAASAIPETATLIDMSTVGPSAI
                     ASAAERLSVPVLDAPVLGSVPSVETGKLVILAGGDRAVFDRHAELLWILGTPIYLGPS
                     GSGAWLKLVNNAASTATLVALGELLALTDRAGLGI"
BASE COUNT         1769 a         3582 c         3511 g         1681 t
ORIGIN      
        1 ggaccgcaag tgggcgcaga tccggctcga gggccaggcg ttcggcgacg tgccgctgaa
       61 cgtcgagctc aagctcgagg tctgggacag cccgaactcc gccggcatcg tgatcgacgc
      121 gatccggtgc gcgaagctcg ccctcgaccg ggggcaggca ggcccgctca tcggcccgag
      181 cgcctatttc atgaagtcgc cgccggtgca gttcagcgac gaggccgccc gcgagatggt
      241 cgagcagttc atcctcggca acggcgcgag caaggacgcg gactggacgg cgttcttcga
      301 catcgagccc gcggaggcgt aggccaggct caggagagga gcgtcgtcta cgcgcgacac
      361 ggcgtcacgg ctcgaacgac cctgcaggtg cttgccgtta cggctccgtg atcgtcggag
      421 ccctatcggt cagaggggca caccctggag tcagcatccg gacgcccgat ctcccgcctt
      481 cggtgtggtt gcggctcacg ccgcgtgagt tcggcgaact actgaccacc ccgccctcga
      541 cctcagatcg aagccggcgt cgctcgatca ttttggacga gcaagcgatc ctccagcgat
      601 tcgccactga cgaccaagcg gcgggtttca ccgtcgccgc atgcactcga tcgctcgatc
      661 cagccacgcg atcaccttgc tcgcagcggc cgtgctcggc tggtggttca gctcggcggt
      721 ggccgacgca tccgtgtcga tccgcccgca gttcttcgta tatgacgcgt cacccgagca
      781 ggtccagatg gcgcgatggg cggccggccg attcgagatc gccaagctcc gcgtgcccga
      841 ggtggaggtc cacttccacg acgacctgtc ggactgcgac ggtcacctcg gatacgccgt
      901 cgatggacgg gtcgatctgt gcacgagtgt cgtcgacgct cccacgcgcc gggcactcct
      961 ccacgagctg ggacacgtct ggctcgacga gaacctggca ccgtcgaggc aggccgcctt
     1021 cctccggctc cgcgacctct ccgcgtggaa ctcctcgagc gatccctgga acctgagggg
     1081 ctacgagcac ggcgccgagg tcatggcctg ggcactcggc gagcggatcc tgacgccatc
     1141 gatcccggac aacgagcccg agaagctcgg gcccgccttc cggttcctca ctggcggtga
     1201 gctcccggac gcgcccgatc cttgacgcga agcctccgac gcgccggagc gatccagggt
     1261 tcctccagcg atctccaagt acctccaccg atcgttgcgg agatgccacc catccctggc
     1321 tcgaacggtt cgacctggct ggcgcctcct ctgcggcggg agtaccgtcc gtgcggggcg
     1381 aggggacggg acagatgagg gtcgaaccac ccagaggctg gttcgggacg ctcgaaccgc
     1441 gccgaaggtc cgagagcatc gaaccgcggg atcactgatg gacgtgacgc gcgagccggc
     1501 gccgaccgag gtcgcgacgt atggcatcga gagcctgctc ggacgaggcg ggatgggtga
     1561 ggtctatctc gccgaggacc gacggctgca ccgcaaggtc gcgctcaagg tgctctcggc
     1621 cgagttcgcg accaacgctt cgttccgcga gcgcttcctc gccgaatccg agctcgccgc
     1681 gtccatcgac cacccaaaca tcgttccgat ctacgaggcc gacgaggccg gcggatacct
     1741 ctacatcgcc atgcggtacg tcgaagggac cgacctgaag gcccgactcc gacaaggccc
     1801 gctgccggtg gaccaagcgg tcgatctgct ctcgcaggtg gcaggcgcgc tcgatctggc
     1861 tcacgaacgt gggctggtcc atcgcgacgt caagccgtcg aacgtcctgg tcgcccccgg
     1921 cgccgggcac gacggccagg accacgcgta cctcgccgac ttcggtctca ccacgcgcct
     1981 tgcggacggc ggttcgctcg cggacgacgg ccagctcatg ggaaccgtgg attacgtcgc
     2041 tccggagcag atcaccggtg gcgaggtgga cggccgcgcc gacgtgtact ccctcggctg
     2101 tctcctcgtc gaatgcctga ccggcgaacc ccctttccag gggtcgtcgg acattgcggt
     2161 cttgttcgcg catctcgaat cgacgccgcc gcgaccgtcc gagcggaggc cggaccttcc
     2221 cgaggggctc gaccgcgtcg tccgcacggc gctcgccaag gacccgaacg agcggtacca
     2281 aacgtgcctg gagctcgtga cggcggcgcg agccgcgctc gggatcgccg agcccgcgca
     2341 tcggcggtcg cgggtcgcag ccgttgtcgg cgtcgcggcg atcgcgctcg ttgccacaac
     2401 gatctggctg accatagggg ctggtgacga accgcgcgac aggcttctcc gcatcgaccc
     2461 ctcgaccaac gaggtcgtcg attcggtcga cgtgggtgac cgtgcgagct cggtcgacat
     2521 cggtgacggc caggtgtggg tcacgagtca ggccggtcgc gctctctggc gcgtcgatcc
     2581 ccgaaccctc gagacgcgtg agacgccggt cgagggaacg ccgatggatg tcgtcgagcg
     2641 caacggcctg gcagtcgtcg tcagcggccc gctcgaggtc gccgtccacc ggatcgacgc
     2701 ggccacggga gctctcctcg agacgatccc gatcccgggg gcggagggcg ccgccacggc
     2761 cgtcgccgaa ggcgacgagg ggatctgggt ggtgggctgc ggcctcggcg ggggcaacgt
     2821 cgggcgtgtc agcgagaccg ccttcagcgg caacagcctc ctcgaccgcg tcgagatctt
     2881 ctcgaagaac ccgaacttcg tcttcgggca cgaaccggac ttcccggcgt acacagacgt
     2941 cgccgtgggc gagggcggtg tctggatgac cctcgactcc gggcccgctc tccgccgggc
     3001 cgacccgacc gggcagcgtc ccgtcgaggt gattgagctc cccttcttcc cgaggtcggt
     3061 gaccgtcgga gccggctcga tctggatcac gggcctcatc gacgacgtgg tcgcccgatt
     3121 cgatcccgag acccgtgagg tgacgatgac cgtcccattg ggacgaggga ccgcgggggt
     3181 ggccgtcggg gcgggctccg tctgggttgt cagttcgatc gacgggaccg tgacgcgatt
     3241 ggacccgcga acgggcgaga tccaggctac gatcgaggtc gacggggcgc cggaagacgt
     3301 cgtgatcggg gcgggcggcg tctgggtcac gagcgacccc gatgcctagg cggatcctcg
     3361 cgctcacttg ctcgctggtg gtcctcggcc tgacgtcgtg cgatcgtccg caacccgacg
     3421 cgatccgcat cgggatcctc gccgattgcg acaccttcgg tgcggcgttc tacgacgtgg
     3481 cacttgcggg tgcggagctg ccgctcctcc ggcgaggtgg ttcgctggat ccctcacatc
     3541 tcgcagacgg cgtcgaggga gtgtcggtcg ggcggcatcc catcgaactc gccttcggct
     3601 gcgcagggga tccggtcgga accgcgacag agacacggcg cctcgtcgag gtcgaaggcg
     3661 tggaggtggt catcggaccg aacgtgtccc caaacttcac gctgatcgac tacgcgaggc
     3721 ggcatcccgc tagggcgttc gtcctcgcca cgatggagca gctcggtgat gcaccaccca
     3781 atgccttccg attcacggcc agctccgtgc aaatgggcgc gggcctcggc agctacgcct
     3841 tccgcgagct cggctggcgc cgcgcatcgt cgatcgccgt ccccgacttt tggcagtggg
     3901 gtatctacgc ggggttcctt gccgagttct gttcgctcgg gggaaagatc cccgatcgct
     3961 actggctcga atcgacggac gaagagatca cggccggcct cgcagagatt cccatcgaga
     4021 ccggcgacgg cttcttcctc tccgtgaaac ccgccgacgc ggcgacgttc ctccggcgct
     4081 actcggcgga ccatccgtcg ctctcccagc gggtgatctt gggggcctat tccagctatc
     4141 cgatcagcct cgatcccgag gtcgtcaaag gggcaggcga cagcttgatg ggcgtcgtca
     4201 cggcgacgtt cgtccccctc gatcggtcgc aggacgactg gaacgcgttt gtctccgagt
     4261 tcgggagctc gttccccgcg ctggccgacg ctgcagactc cacctatcac ctgttcgaca
     4321 tcgactacgt caacgcgatg gaggcggtga tgcaggctct cgaggccgtc gacggcgacc
     4381 tctcgcatcg cgccagcggg ttccagttcg ccctctccga cgtcagactc gatgcgccga
     4441 acggcacgat ctcgctcgac gagcgaaggc aagcgatcct gccgatctat ctgtcgcggg
     4501 tcgaaagggc cgacgatggc gatctcgtcc ttcgaacgtt caggaagatc gaggcggtag
     4561 atcaaagtta tggaagtctc ctcaacgcgg acgtaccggt gccggatcgg acctacccac
     4621 cttgcgtcga aggagacccg ccgccctggg caaccgccgt cagctgacgg gcgggccgac
     4681 ggaccaggtg aagcgcgtga ggctctcccg cggcgcgagc ccgccggggc gtaggccgcg
     4741 gcgcacgaca cacgtgggtt gcgccgcacc tctcaccagc gcgagccgca atggcccgag
     4801 cccctcccgt tgccaccggt cggacagacc gacgaccagg accccggcac cttcggctgc
     4861 cgagagcatc ccctcgcctc cgggcgccgc cagcacgggg tcggtggcga tccccaccgc
     4921 tcgttggatc gcgaccgacg cgtggaacag gagcatgctg gcgtccctct tgcccgcatc
     4981 cggaaaggcg tcggccccga taagccggag cgggagaccg cgagctctcg cgtaccacgc
     5041 gccgagctcg atggccgccc agtcgtgctc ggtccctccg aagggaacga cgaccggccc
     5101 cgcagcgggc ccgcccgctc caaccagcag cgcgacgtcg cagggagcgt gcgagaggac
     5161 gcgagcgacg tccgtgtcgg ggagcccgtc gcggacgagc gcctggggcg ggtcaacgat
     5221 caacaggacg acgtcctgct ccgacgacaa ccggaccagg tcctcacccg gcccctggga
     5281 ggtgaaggcc gcggctcggg cggtgacacc gcgcttctcg agcgccgcca cgccatcgtt
     5341 gaggcgcgcg gtctcggcaa cgagttcccc ctgatcgcga acgagtcgcg cgatcaccag
     5401 ctcgaagcgc ccgtcgcgca cgagcgccgc tccgagatcc agcatcgtgg ggaggcgtga
     5461 tccatccaga actgcaacca ggacgacccg ccgcggctcc gacagctcgg gcaaacgttc
     5521 accaccggag accgagagat ccagagagct gtcatgatcg aggatcgact tttcgaggct
     5581 ctgcaacgca ggactcggat cgatccccaa ttcctcggcc agctttgcgc gggcggcttg
     5641 atagctggcc agggcatccg tctggcgctc ggaccgatag agggcgagca tgtgctgcgc
     5701 gcggaatctc tctcggaagg ggtactcctc gacgagcgcc gcgagctccg ccgcgacctc
     5761 accgtgacgc ccacacgcga ggtcggcatc gattcgcttc tccaacgccg cgagacggag
     5821 ctcgtccaac ctccccgcag caacgacgac gaacggttcg tcggagatgt ccgcgaacgg
     5881 cggaccccgc cacagcctca gggccttgct gaaggcttca accgcttcga gcggcctatc
     5941 gttagcgagc gcgccaccgc cttcgtcgac gagtcgctcg aaccgacgta gatcgaggtt
     6001 gctcggctcc acatggagca catagccgcg tccgcgggtg gcgatggcct cgcggccgat
     6061 cacctgacgc aggccggaca catatccctg gatgatgttt gctgcgctct ccggtgggtc
     6121 gtcgccccac agatcgtcga tcaggcgacc gaccgggacg acctcggtcg cgcgcaggag
     6181 gagggaggcg agcatcgcgc gctgcttcgg tccaccgaga gagagttgcc ggccgtcgtc
     6241 gaatgcctcg agcggcccca agacccgata gtcgacggcc acgtcgggac cgtagcaccg
     6301 gacgctctcg aaagctgcgg cgcaccgaac accgacggtg gggcctcccg ggggaccctt
     6361 cagcgttcgt ccagcctgcc tacagcgccc cgagtcatcg tctcctcgaa cggagaggag
     6421 gtgtcgaaga tgctcggagc aggagtccca ccggtcggga tcccgaccca cccaccggcg
     6481 gacggcacgt cggtcgcgag cgtcgtcctg atgatcgtgt tcgtgaccgc ggtcgctttg
     6541 gcgatcgcga tccaagtgcg cgcgggtcgc cggaacgcga ccggggatcg ggacgcgggc
     6601 ccggatgagg aggtcgaacc ccgtcaggtc gaggagttgc tcgctcgatg accgtgcgcg
     6661 gcgtcggtcg cacgctgcgg tggaccgtcg acgcgcgtcg cggtggagcc tcgacgaacc
     6721 ggtggagcac ggttggggcg ggtcgagatc cgaccagcct caaccggctc catgagatcg
     6781 tgatcgaccg tccgaccata gggtgcccag ggttcccaag ggatccgagc gggccgaacc
     6841 ggtcgaatcg gggctcgacc gaggcctcac cgaggtctac ctcgtgtcac ggcaggaaac
     6901 ctctccagcc ttgacggtct gtatctaagt cgagtcccag tggggccatg ttggtcggtt
     6961 cacgcgaaac gcggagggaa tcaccagtcg aggaggagag agacggatgc aatctgcgat
     7021 gcacccgcat gaacatcacg attccgagct tccgatccat agctggaggc tgaccgcgac
     7081 gtgcgaacac ggcgtcgact acaaggtctc tcccgaacaa cttgttcgcg ggtccctccc
     7141 tccgtgcacg tgcagggcgg aatcggaagc gatgggacgc cgagggcctt ggtcacagga
     7201 acgccgaacg atgagacgct tccgccgcga actgaggtcg ttatgaaggg agtcaccaga
     7261 tgcgccgttt cgcgttgatc ttcgccccgc tcctcatcct tctcgggctc gcgggtcccg
     7321 ccatcggggc cgtcaccgat gacttccaag cgcagttcac gcaacacttc gggcgtggga
     7381 tcggagccga cagcggacag tgcgcggtcg acttctgcgg cagcggccgg gtcgtgggct
     7441 acggcgaggc cgagctcacg atcatcgcca cgagcgccgt cccgacatca cgggagacgg
     7501 aggcccggcc acggacgcgc cgatccacac gcctgaacag ttgtgggtgg acgacgaggg
     7561 gaacatctac gtcgcggata cctacaaccg ccgggtccga aagatcgaca cggacggcat
     7621 catcaccacc gtcgcgggca acggaacgca ggccttctcc ggtgatggcg ggccggccac
     7681 gtcggcaggg cttgcgaaga tctccggcgt agcaatcggt ccggaaggag ccctgtacat
     7741 cgccgactcc gcgcatgaca gggtccggag ggtcctcctc taggccacgc cgacccggca
     7801 tctctgtaaa cctcaacgac ccctcccgac tgtcttgagg tatcgaatcc gggataggaa
     7861 agagagcatg cccaggtcgc catccatctg cgctgttgta accctgatcg cgtccctctt
     7921 ggcagcggac gccgaccacg cgacagccga gagagggatc aaagagaggc tggcctcagg
     7981 gcaaagtctc caaccggcac tcgccgtggt aagcgaagac ccgtacacca acccagggac
     8041 gtatcaccgt acgcaagtcg agccggacag cttcgccttc ggctcgaccg tggtgagcgt
     8101 cttccagtcg ggacggtcct accggtgcgg cgcatccaac atcggctggt ccgtatcgcg
     8161 cgatgcgggc acgacgtgga cggacgggtt cctgccgagg acgacgcccc actcggcgcc
     8221 acaaggccca tggaagcgcg ccacggatcc ggtcgttgcc tacgatgcgg cgcatgacac
     8281 atggctcgcc caggtactcg gcatcccgtc gtgtcccttc gcgggaggcg atgtgatcgt
     8341 gagccgttcc accgacaacg gcgccacgtt cggtgagccc gtgatcatca agcgtcagca
     8401 ccgcacgcag gggttcgaca agaactggat cggttgcgac aacttcccgg acagcccctt
     8461 ctacggcacg tgctatgcgg cctgggacga ctaccgccga tatggcttcc tctacggcga
     8521 gggcatttgg catcccctca tccacgcgta cagatcgacg gacggcggcc tcacctggag
     8581 caaggcagcc cttcccgaag acccatgcgg tgtcgttaac cagatcgtgg tgcagccaag
     8641 tgggaaggcg atcatccagt cgaacgattg cagccctcgg caccaggtgt tcgtgtccga
     8701 ggacggtgga cggacctaca agggaccgtt ccgcatctcg acgagccgtg tacgcggcgc
     8761 cgctggcatg ctgaggagtc cgtacttcat gtcctcggac gtcgacgcga gcggcaggat
     8821 ctacacggtg tggagggact gcacgttccg ctcgttcgaa ggaggcgact gtacccacaa
     8881 cgacctcgtg atgagcacct cgaacgacgg tcgccactgg tccgacgtcg tccgcatccc
     8941 aatcgaccct cgaagcagct ccgtcgatca cttcctgcct gcgctcgccg tcgatccacg
     9001 aacgtccggc gcaacggctc atgtcgccat cgcctactac ttctaccctc agcaacgctg
     9061 cgacgcgacg acctgcgacc tctccgtcgg gttcacgtcg tcggtcgatg gtggttcgac
     9121 gtggtccgtc caggagctcg ccggaccgtt caagaacaca tggttccctc tcacgacgag
     9181 cggctacagg gttggcgact atatcgggat ctcgttcgtc gacggcaagg ccatcccggt
     9241 gttcatcgtc gggtcggagg gcgagtgcga gctcggcgac gtgacctcgt gcaacgtgtg
     9301 gaccgcctcg gcgacgatct cgctcgcatc ctgagggaga agcacaccca caacgaggag
     9361 tcgttgctcg aggagtcttc cctcccacca tgtccgtcct gtcgagtgaa atcgggtggg
     9421 acgacagatg acgcaaccgg ctgctaggac aacggtgtcg ctcgtacgca gaaggatcga
     9481 tgtctggcgg tcgcatcgtt gccacgatct cccgactgga tagagttccc gactcgacgg
     9541 aaccagaagg gagtctccgt gcagacctca cgcgtgaacc ccgcagccgt tcatccaccc
     9601 gtcggtacct actcgcactc ggttcgcgtg gggaccgccg acggcacctg gatctgcgta
     9661 tccgggcagg tcgccttcga cgccgagggc acgctcgtcg ggcggggaga catgcacgcc
     9721 cagacccggc aggtgttcga gaaccttcgg gcgatcctgg cggcgaacga tgcgtcgttc
     9781 gcagacgtcg tcaagattgg gacctatctc acgacgctcg acgacctcgc cggcatgcgc
     9841 gagatccgca gcgagtacct gacgtcggag ccgccggcga gcacggcggt gcaggtggcg
     9901 ggactggtag tccctgacgc gctgatcgag atcgagctcg tcgcggtggt gccgacatga
     9961 ccgcggtcgc ggtcctcggg ctcggcgcga tgggacacgc ggtcgcggat cggctgctcg
    10021 gcgcccgtca tgacatccgc gtctggaacc ggacgccggg cagggacgac gacctcgtcg
    10081 cctccggcgc tggccggtcc acgacaccct cggacgccgt tcgcgacgcg gaggtcgtca
    10141 ttacgatggt gaccgacccg ccggcgctcg aggaagttct gttcggaccc aatggagccg
    10201 cgtccgcgat acccgagacc gccacgctta tcgatatgtc gacggtgggt ccgtccgcga
    10261 tcgcatcggc ggccgaacgg ctctccgtcc ccgtgctgga cgcaccggtg ctcggcagcg
    10321 tcccgtcggt cgagaccggc aagctcgtga tcctggcggg cggggatcga gcggtgttcg
    10381 accgtcacgc cgagctcctg tggatcctcg ggacgccgat ctacctcgga ccgtcgggct
    10441 cgggcgcgtg gttgaagctc gtcaacaacg cggcgtcgac cgcgacgctg gtggccctcg
    10501 gcgagctgct ggcgctcacc gaccgcgcgg gtctggggat cga
//
LOCUS       DASUYD010000259         3828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_430982, whole genome shotgun sequence.
ACCESSION   DASUYD010000259 DASUYD010000000
VERSION     DASUYD010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430982"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /gene="nadE"
                     /locus_tag="VF195_13540"
     CDS             <1..497
                     /gene="nadE"
                     /locus_tag="VF195_13540"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX6581875.1"
                     /translation="SNKFGSLVLATGNKSEYAVGYATLYGDMAGGFAPIKDVPKTVVY
                     ELAKWRNAIGGAETPPIPQRVLDKPPSAELRPDQKDTDSLPPYDVLDPIVEAYVEDDR
                     SPEDIIAARGLDPKLVDRVVEMIDRAEYKRRQAAPGIKITPKAFGRDRRLPITNRYGR
                     FRTP"
     gene            510..584
                     /locus_tag="VF195_13545"
     tRNA            510..584
                     /locus_tag="VF195_13545"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:545..547,aa:Lys,seq:ttt)
     gene            1343..1702
                     /locus_tag="VF195_13550"
     CDS             1343..1702
                     /locus_tag="VF195_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581876.1"
                     /translation="MDVGLIIRYGAIVPGRETQAIEVFDEATKYYEEKVAQKVLTYFE
                     PFFFMTSDYQEETGFFILKGPAPEVFKLMEEEKYRWLLQKAGLLVEHLRVDYLTVGEG
                     ITEQLERFAKVRVDLGI"
     gene            complement(1977..2447)
                     /locus_tag="VF195_13555"
     CDS             complement(1977..2447)
                     /locus_tag="VF195_13555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX6581877.1"
                     /translation="MVRVDGEIVINRPVEEVFNFVADERNEPRYNARMRRAEQISEGP
                     IGLGTRYRAELVSMGRAIRMEIEFTGYERPRRLASSTHMSSMDTQGALTFDPIPGGTR
                     MRWSWELEPRGVLKLVGPVVAWMGRRQELRIWTSLKGLLEGPGTTHRAGPSPNS"
     gene            3247..3582
                     /locus_tag="VF195_13560"
     CDS             3247..3582
                     /locus_tag="VF195_13560"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6581878.1"
                     /translation="MRRIDLVVPRGAILRAQAPETRRERMRGLLGRPSLGRDQALLLE
                     STRSVHTFGMGFPIRVALLDHDHVIRRILDVPANRLLRPRRGVRHVLEYAEGADLRPG
                     DRLRIVQAN"
     gene            3657..3729
                     /locus_tag="VF195_13565"
     tRNA            3657..3729
                     /locus_tag="VF195_13565"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3691..3693,aa:Glu,seq:ttc)
     gene            3748..3823
                     /locus_tag="VF195_13570"
     tRNA            3748..3823
                     /locus_tag="VF195_13570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3784..3786,aa:Asp,seq:gtc)
BASE COUNT          633 a         1254 c         1279 g          662 t
ORIGIN      
        1 tctcgaacaa gttcggctcg ctcgtgctcg cgaccggcaa caagagcgag tacgcggtcg
       61 ggtacgccac gctgtacggc gacatggcgg gtgggttcgc gccgatcaag gacgtgccga
      121 agacggtcgt atacgagctc gccaagtggc ggaacgcgat cggtggagcc gagactccgc
      181 cgatcccgca gcgggtgctc gacaagccac cgtcggccga gctgcgtccg gaccagaagg
      241 acacggactc gcttccgccc tacgacgtcc tcgacccgat cgtcgaggcg tacgtcgagg
      301 acgaccgatc gcccgaggac atcatcgccg cccgtggtct ggatccgaag ctcgtggacc
      361 gagtcgtcga gatgatcgat cgggccgagt acaaacgccg ccaggcggct cccgggatca
      421 agatcacgcc gaaggcgttc ggacgcgacc ggcggctgcc gatcaccaat cgctacgggc
      481 gtttccgcac cccgtagact tcgccatcag gggcggtagc tcagcctggt cagagcaggg
      541 ggcttttaac cctcgtgtcg cgggttcgaa tcctgcccgc cccacgccac gaagtcgctg
      601 gtagacggct cgttcgcgtc ggctctcgtc gcagctcgac gactctcgtc ccgccctggt
      661 cccgcatctg tcccgcgcgc gtcgcctgga tggtcgcctc gaggcggtcg caaaaggccc
      721 tggtggaggc tctcgaatag tcattcaaag tggttgtgat cgagcgagga cggcagagtc
      781 cctggatctg cttggggtgc gggcctgccg cgatcgcgag tgccactgcc gtatgtcgaa
      841 ggccgtgcgg tgtgacctcg gctcgggagt catcgatcac gtcgctctct acatcggcgg
      901 ggtcagacca tcgcctccag caatccaagc tcgcctgtcg ggtacagggc catcgattgg
      961 gacgcggttg tcgagccgcc cgaccaggat agtcaacgct cacagtcgat tcatccaacg
     1021 ccatcgcggt ggcgcctgcc ggcgagtagg gccacccggc cgaggcaccc acgtggcgat
     1081 cgccctaaca cgtgtagttc tcgacggcct cggccagcgg agccgatccg gatcgacccg
     1141 ccccaacggt ccgtcgagcg tgacgaacgc gatctcccct cgtctcgagg tccacgggga
     1201 gggtcccggc caagacgggt ccaccggccc tgccttcggc gaagctccgg ggccacgatg
     1261 agtggcggtc ctgttcccgg ccgggaaggg gtgacgggga acacccggtc ggacccggaa
     1321 ggtgccagat cgaggaggag tcatggacgt tgggttgatc atccgctacg gcgccatcgt
     1381 gcccggtcgc gagacccagg cgatcgaagt gttcgatgag gccacgaagt actacgagga
     1441 gaaggtcgcc cagaaggtac tgacgtactt cgagccgttc ttcttcatga ccagcgacta
     1501 ccaggaggag acgggcttct tcatcctgaa gggcccggct cccgaggtgt tcaagctgat
     1561 ggaggaggag aagtaccgtt ggctcctgca gaaggctggc ctgctcgtcg agcacctgcg
     1621 cgtggactac ctcacggtcg gtgaggggat cacggagcag ctcgagcgct tcgccaaggt
     1681 ccgcgtcgac ctggggatct gagcgccgat cgtccacgct gagaccgggg taatccgggg
     1741 cgcatcccgg tctcggcgtg gggccgacgt ttgaccccgg aagaagtcct caaaggccgc
     1801 cgctggtccg ccacgatggc ttcgctcggg ctcgggtggg cggagatcgc cggatcgttc
     1861 atcacgccag attcaacgcc tggcgaggcc gaatcgtttg gttcggcggt cagtgatcgg
     1921 tctcccgcag catcccgacg agcacggccg cagcgtccgg tcctactcgc aatcggtcag
     1981 gagttcggcg aaggtccggc tctgtgggta gtccccggcc cttcgaggag gcccttgagg
     2041 ctggtccaga ttctcagttc ctgtcgccga cccatccatg cgaccaccgg ccccacgagc
     2101 ttgagcacgc cgcgcggctc aagttcccac gaccaccgca tccgtgtccc tccggggatg
     2161 ggatcgaacg tcagggcgcc ttgagtatcc atggaggaca tgtgggtcga tgacgccagt
     2221 cgccggggtc gctcgtagcc ggtgaactca atctccattc tgatggctcg gcccatgctc
     2281 acgagctccg cccggtaccg cgtgccgagg ccgatgggac cttcggagat ctgctcggcg
     2341 cgacgcattc gggcgttgta gcggggctcg ttgcgttcgt cggcgacgaa gttgaacacc
     2401 tcctccaccg gccggttgat aacaatctca ccgtcgacac ggaccatctc atctcacctc
     2461 ccggccgcgg tactagcctt tcccctcacc gttcactcga tctagctcca gaaactcgcc
     2521 gatccgctcg gcgcgcttga agtcgggagc atgaggacgt ggagcaactc ggcgcgtgga
     2581 gcgggaccga gggcgtctag acgggcctgg agccgtcgtt cgatttcatc tgcacacaca
     2641 cttggagacc agcccctcgt gctcgtcgcc tgctcgctca taccgtggac ggctcgttcg
     2701 aggatgtcgc gctgacggcc agccacggcg ggatgttagc ccttgcaccg cgacaagcgg
     2761 gcgttgttga gagccggagc gatgacagag acttcgtttg ctgaaggtgc ccaggccgcc
     2821 gctgcgttga tccaagcttt ggcgctggtc gtcgggggga ggttgggcgt acgtccgctt
     2881 cgtcaagaga cacgaactca ggccagaggc aaaccttcat ctcgatatcg cgcatagacc
     2941 tccgtgcact tcgcgtatgc ggacgagtcg ggcgatgccg ggcaatgagc ctgtcaccgg
     3001 attctcctcc cgcatctgtc cggtctccgt cccgcgaacg accgggaaac gacgcgagac
     3061 gacgagttgc accgagtagg caaacgcgca ggtcaccttg ctgtcggccg gttcacgctg
     3121 gtagcggaat cggcggggag ggggctttga ccctcgtgtc gcgggttcga atcctgcccg
     3181 ccccaccaca tggatcgtgg gcgtcgtgga acgtgggcgt cgtggggtcg cgatagggtc
     3241 gcctcgatgc gacggatcga tctggtcgtt cctcgcggcg cgatcctgcg ggcccaggcg
     3301 cccgaaactc ggcgcgagcg gatgcgcgga ctgctcggtc gtccgtcgct cggccgcgac
     3361 caggctctcc tgctggaatc cacgaggtcc gtccatacgt tcggcatggg gttcccgatc
     3421 cgggtcgcgc tgctcgacca cgaccacgtg atacgacgga tcctggacgt accggccaat
     3481 cggctcctgc ggcctcggcg cggggtccgc cacgtcctcg agtatgcgga gggcgccgac
     3541 ctccgaccgg gagaccggct ccggatcgtc caggccaact gaaaggccca gccgagcggg
     3601 ccgttatact tcccggcgct gcccccgggc cgggggcggg gtgcgggatg gaccgggccc
     3661 ccatcgtcta gcggcctagg atttcgccct ttcaaggcgg cgacacgggt tcgaatcccg
     3721 ttgggggcac acaaccgaat agcacacgcg cccgtagagg agtccggccg tcctcaccgc
     3781 cctgtcaagg cggagatcgc gggatcgaag cccgtcgggc gcgccgat
//
LOCUS       DASUYD010000260         2401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1092005, whole genome shotgun sequence.
ACCESSION   DASUYD010000260 DASUYD010000000
VERSION     DASUYD010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1092005"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="VF195_13575"
     CDS             <1..348
                     /locus_tag="VF195_13575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581879.1"
                     /translation="AAEGSFALCACANVSTSTELAARRFIQDYQSEHGVAPGAYAVEG
                     WDAAHVILRAVGDGGVTRSVVSSSLDDLTVVDGLEATHRFDANGEPLEPRALLRLYRV
                     EGGRWVPAGWEGI"
     gene            453..1334
                     /locus_tag="VF195_13580"
     CDS             453..1334
                     /locus_tag="VF195_13580"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEX6581880.1"
                     /translation="MKGTRLWLAALIAVFALIVAACAGNDDEGPTGGTGVTAETGGTG
                     ATGETGFPAFTTLEEGVLQVGNCLDYPPFESVVGGDEVGFDIDLVEEVAGRLGLEVEW
                     IRADFDTVFTAVAGDQFDAVAAAATILPEREDIVDFSVPYYNSRQSLTVNTQETPDIT
                     TTDQLGEGDVVAVQKGTTGKIWAEENLVPNGVELKTFQLASDMFRDLEAGNVQGIIND
                     EPASAEIVKDLPALEVVQAIDTNEKYGVAFSPSNPELREAFNLAFEQVVADGTYADIL
                     LEYFPGVDVPEEFQPTA"
     gene            1410..>2401
                     /locus_tag="VF195_13585"
     CDS             1410..>2401
                     /locus_tag="VF195_13585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX6581881.1"
                     /translation="MSLTEAPTLDRDVAPDVRGPTAPLPVTILALGIASLVVVTLGVL
                     APQVNYAMSDRVLTANCIDQQIAPEDNGAIGVCNIVEALDDPLTSWSLYVGIALGALA
                     LALGILTYRRMDTERRRDHAITGAVLGSQGILLAVGTLWFRSTSPALFAKHFLNFADL
                     EGYGGAFVRGARNTLTLALAGELGGILIGLILGVLTLSRRRSIRAPARIYINFFRGTP
                     LIWQLVAGYFLLLFGFGVRLSAFTAAIIVFALNTGAYAAEVFRAGIQSIERGQIEAAR
                     SLGMSYSQAMRYAIVPQAVRRVIPPLMNEFVILIKDTALVIALGLTAANYDIFTT"
BASE COUNT          380 a          768 c          862 g          391 t
ORIGIN      
        1 gcggccgagg gctcgttcgc cctctgcgcc tgcgccaacg tctcgacctc gaccgagctc
       61 gccgcccgcc ggttcatcca ggactaccag tccgagcacg gggtggcgcc cggggcctac
      121 gcggtcgagg gctgggacgc agcgcatgtg atccttcgag ccgtaggcga cggcggggtc
      181 acgcggagcg tggtctcgag ctcgctcgat gacctgacgg tggtcgacgg gctggaggcc
      241 acgcaccggt tcgacgcgaa cggggagccg ctcgagccga gggcgctgct caggctgtac
      301 cgggtcgagg gcggccgatg ggttccggcc gggtgggagg ggatctgatc ggggaccgga
      361 gcgcggcggt gggttgagtc gggcacccga ttcgggggag gatggcggct cgaacgctta
      421 ggcacgacgg gggtagggag aagggggacg cggtgaaagg aacgaggctc tggctggcgg
      481 cgctgatcgc ggtgttcgcg ctgatcgtgg cggcgtgtgc gggcaacgac gacgagggtc
      541 ccaccggcgg gacaggcgtg acagccgaga ccggtggaac cggcgcgacg ggtgagacgg
      601 gcttcccggc gttcacgacg ctcgaggagg gagtcctcca ggtcgggaac tgcctggact
      661 acccgccgtt cgagtcggtc gtgggcgggg acgaggtcgg attcgacatc gacctggtcg
      721 aggaggtcgc cgggcgactc ggcctcgagg tcgagtggat ccgtgcggac ttcgacacgg
      781 tcttcacggc ggtcgccggg gaccagttcg acgcggtggc ggccgcggcg accatcctgc
      841 ccgagcgcga ggacatcgtc gacttctcgg tgccgtacta caactcgcga cagtcgctca
      901 ccgtcaacac gcaggagacg cccgacatca ccacgaccga ccagctaggt gagggcgacg
      961 tcgttgcggt gcagaagggt acgaccggca agatctgggc cgaggagaac ctggtgccca
     1021 acggggtcga gctcaagacc ttccagctgg cctccgacat gttccgcgac ctcgaggcgg
     1081 ggaacgtgca gggcatcatc aacgacgagc ccgcgtccgc cgagatcgtg aaggatctcc
     1141 cggcgctcga ggtggtccag gcgatcgaca ccaacgagaa gtacggggtc gccttctcgc
     1201 cctcgaaccc ggagctgcgc gaggcgttca acctggcctt cgagcaggtc gtcgccgacg
     1261 ggacgtatgc cgacatcctg ctcgagtact tcccgggggt ggacgtgccg gaggagttcc
     1321 aacccacagc ctgacgacgg gcggcgtacg acgggaggtc cgcgcccccc gggggcgcgc
     1381 acctctcgct ctggacggag gtggagagca tgagcctgac cgaggcgccc acgctcgacc
     1441 gggatgtcgc accagacgtc cgaggtccga ccgctccgct gcccgtgacg atcctcgccc
     1501 tcgggatcgc ctcgctcgtc gtcgtcacac taggtgtgct cgcaccgcag gtgaactacg
     1561 cgatgtcgga ccgcgtcctg actgcgaact gcatcgacca gcagatcgca ccggaagata
     1621 acggcgcgat cggtgtctgc aacatcgtcg aggcactgga cgacccgctc acgagctggt
     1681 cgctctacgt cggtatcgcg ttgggcgcgc tggcgttggc gctcggcatc ctcacctacc
     1741 gccggatgga cacggagcgt cggagggacc atgcgatcac cggcgcggtc ctcggatcgc
     1801 aggggatcct gttggcagtc ggcaccctct ggttcagatc gacgagcccg gcattgttcg
     1861 cgaagcattt cctcaacttc gccgacctcg aggggtacgg gggagcgttc gtccggggag
     1921 cccgcaacac cttgacgctg gctctcgccg gtgagctcgg aggcatcctg atcggcctga
     1981 tcctcggtgt gctgacgctg tcccgtcgtc gatccattcg ggcgccggcc cgaatctaca
     2041 tcaacttctt ccgcggcacc cccttgatct ggcagctcgt cgccgggtat ttcctgttgc
     2101 tgttcgggtt cggggtccgg ttgtcagcgt tcacagcggc gatcatcgtg ttcgccctca
     2161 atacgggtgc gtacgccgcc gaggtgttcc gcgcgggcat ccaatccatc gagcggggac
     2221 agatcgaggc cgcgcgttcc ctggggatga gctactcgca ggcgatgcgg tacgcgatcg
     2281 ttccgcaggc ggtgcgcagg gtgatccccc cgctcatgaa cgagttcgtg atcctgatca
     2341 aggacacggc cctcgtgatc gcgctggggc tcacggccgc gaactacgac atcttcacga
     2401 c
//
LOCUS       DASUYD010000261         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_433801, whole genome shotgun sequence.
ACCESSION   DASUYD010000261 DASUYD010000000
VERSION     DASUYD010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433801"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..763)
                     /locus_tag="VF195_13590"
     CDS             complement(<1..763)
                     /locus_tag="VF195_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581882.1"
                     /translation="MSSPEGSTDVYRLLAEDIDANGARPNGDVWERIVHLVDPTELRP
                     KLAEDIEIRVFEQRWGDGYVMIANPRDLLHYRLAASDLGLLQMMDGTRTVKEIVIERF
                     EGSGEMELSTVIDLVRSLYRGNFLEQRYVDVPSMVTRAMDPVTERRRKARQFGRTLSI
                     EWEGAQRLVKWLYDHGLKWVFRPAVLVVLIAAAVVGLFFFVDLVRHQRFSIAGRSLAI
                     GFIVLIVLDYLMVFCHELGHAVVIVHNGRRIRSAGF"
     gene            complement(760..1542)
                     /locus_tag="VF195_13595"
     CDS             complement(760..1542)
                     /locus_tag="VF195_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581883.1"
                     /translation="MTASGDDAHDSRVTSVTLHAGPSRSKMRPVIPRFALGASLIVAA
                     LMAVPVSAGPAAAQSVSPSPSRGPGVAGQVTGALMAGSRLIIRVDATMPGGWQALHLV
                     EVAIVSGNRDLEHLRFDIEDNKLTVGEQSNVIVGTGAVATGTHLRVSGADVVVTTGGA
                     NLSFEVDTRVIETIPQDARFELSVVDDLGTSAQVTRTLAEPESSGITWGTVVALIAAA
                     LFAGGFVGNMFASKRRQPARGSVYDAVQRRLESDRAGKGRSS"
     gene            1588..1758
                     /locus_tag="VF195_13600"
     CDS             1588..1758
                     /locus_tag="VF195_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581884.1"
                     /translation="MLSGEMARHQIADRVREAEAERLARSTRKGKAMESRGITRRIGR
                     AALAAATWPIKH"
BASE COUNT          351 a          710 c          664 g          296 t
ORIGIN      
        1 ggaacccggc gctgcggatc cgccgcccgt tgtgcacgat gacgaccgca tgcccgagct
       61 cgtgacagaa caccatcagg tagtcgagga cgatcagcac gatgaagccg atcgcgagcg
      121 aacgacccgc gatcgagaat cgctgatgtc gcaccagatc gacgaagaag aagagcccga
      181 cgaccgccgc cgcgatcaac acgacgagga ccgccgggcg gaagacccac ttcaggccgt
      241 ggtcgtagag ccacttgacc agccgttgcg ccccttccca ttcgatcgat agggtcctac
      301 cgaactgccg ggccttgcgg cgtcgttcgg tcaccggatc catggcacgc gtcaccatgc
      361 tcgggacgtc gacgtagcgc tgttcgagga agttgccccg atacaacgaa cgcaccaggt
      421 cgatcaccgt ggacagctcc atctcgccgg aaccctcgaa ccgctcgatc acgatctcct
      481 tgaccgtgcg cgtgccgtcc atcatctgaa gcaatcccag atccgaggcc gcgagtcggt
      541 agtggaggag atcgcgaggg ttcgcgatca tcacgtaacc atcgccccag cgttgctcga
      601 acacccggat ctcgatgtcc tccgcgagct tgggccggag ctcggtcgga tcgacgaggt
      661 gcacgatccg ttcccagacg tcgccgttcg gcctcgcacc gttcgcatcg atgtcctcgg
      721 cgaggagccg gtagacgtcg gtcgaaccct ccggggagct cacgacgagc ggcccttgcc
      781 cgcccgatcg ctttcgagcc gtcgctggac cgcgtcgtag accgaccccc gggcgggttg
      841 gcggcgcttg gacgcgaaca tgttcccgac gaagcctccg gcgaacagcg cagcggcgat
      901 gagggcgacc accgtccccc aggtgatccc cgagctctct ggctccgcca gcgttcgagt
      961 gacctgggcc gacgtcccga gatcgtccac gacgctcagc tcgaacctgg catcctgcgg
     1021 gatcgtttcg atcacgcggg tgtccacctc gaaggagagg ttcgcgccgc cggtggtcac
     1081 gacgacgtcg gcgccgctga cgcggaggtg ggtgccagtg gcgaccgcac ccgttccgac
     1141 gatgacgttg ctctgctcgc caaccgtgag cttgttgtcc tcgatgtcga agcgcaggtg
     1201 ctcgaggtct cggttgccgg agacgatggc cacctccacg agatggaggg cctgccagcc
     1261 acccggcatc gtcgcgtcga cacggatgat cagtctggag ccggccatga gggcgccggt
     1321 cacctggccc gcgacaccgg gaccgcgcga tgggctcggc gaaaccgact gcgccgccgc
     1381 gggtccggcg gagaccggca cggccatcag ggcggccacg atcagactcg ctcccagcgc
     1441 gaaccgaggg atcacgggcc gcatcttcga cctcgagggg ccggcgtgca acgtgacgga
     1501 cgtcacccgg gagtcgtgag cgtcgtcacc gctcgcggtc actgcgcgga cgtaccgtgc
     1561 gcgaaggttc cagagggagg ttcggagatg ctgagcggag agatggcccg gcaccagatc
     1621 gccgatcgcg ttcgcgaggc cgaggccgag cgtctggccc gttcgacacg caagggcaag
     1681 gccatggagt cgcggggtat cacccggagg atcggacgcg ccgcgctcgc ggccgccacc
     1741 tggccgatca agcactagcg ttcacggcgc cgacaccggt gcgccgccgg ggcgtcacag
     1801 gcctactgta cggttccaac cgccaacgag aggaacgcgc ccctctcgag cggggggccg
     1861 ggtcaggcac cggccccccg cacccccttc gaccggaccg gatgggttcg gtccgctcat
     1921 ccgcgacgag gccgctcaac ccggccaacg gccgacgagc acgcgggtca ccttgaagcc
     1981 cccgtccatc gtgctcttca cgtccccgaa accgttccgc c
//
LOCUS       DASUYD010000262         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1756179, whole genome shotgun sequence.
ACCESSION   DASUYD010000262 DASUYD010000000
VERSION     DASUYD010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756179"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="VF195_13605"
     CDS             <1..1011
                     /locus_tag="VF195_13605"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX6581885.1"
                     /translation="GIGDATVAAVEAFARDEEIDVMEACRRVDEIATLASRAKGAVAG
                     FNQVMTAIAGHFADGAGPARMVEFAAQESGYLAELEGDRTVEAQGRIENIQELSGVAA
                     ELLARDPDAGIDVFLEQVSLIGEQDDYDEEESSVTLMTLHIAKGLEFPAVFIVGLEDG
                     IFPHYRSMTDSAQLEEERRLLYVGITRAQKRLYLCHAWGRTLFGQTNYNPPSRFLNEL
                     PPNLLDVREEGGSGRGSHPRDASRGGRASRAERGYTVVGLPGKGQVDVAPTGSWSPSP
                     PPKRQVPTVAEGDTVVHERWGEGVVVAIKGHGDDTEATIAFSEAGEKRLLLSYAPLTR
                     VG"
     gene            complement(1027..>2108)
                     /locus_tag="VF195_13610"
     CDS             complement(1027..>2108)
                     /locus_tag="VF195_13610"
                     /inference="COORDINATES: protein motif:HMM:NF042648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N,N-dimethylformamidase beta subunit family
                     domain-containing protein"
                     /protein_id="HEX6581886.1"
                     /translation="SLYTGGTRVSFERPLARGFLDKPEPAVRKAEAVPDREALGYFRW
                     AEEHGLSPWSGGAGWWTWERPFVAWAEASGYEIDIAVSQDLERHPEVLDGHRMFLSVG
                     HDEYWSWGMRDTVETFLAGGGNAAFFSGNTCWWQVRFDDRHRGMTCFKYRAAEDPVLG
                     TPEERSLTGAWADRRVGRLEAAMTGLSFSRGGYSRYGLGVPRASGAYTVWRADHWAFE
                     GTDLRYGDALGLADAIVGYEVDGCELTLEDGLPVPTHMDGAPDTLQILATAPATLWSQ
                     DEQPSRYADEPGELEHVAQALFGDGWQEELHRIRHNHAVVASFDHPGGGTVFNAGCTD
                     WTYGIEGGDASVRRITRNVLERLSA"
BASE COUNT          318 a          728 c          757 g          305 t
ORIGIN      
        1 gggatcgggg acgccacggt ggccgccgtg gaagccttcg cacgcgacga ggagatcgac
       61 gtcatggagg cgtgccgccg tgtggacgag atcgccacgc tcgcgtcgcg ggcgaagggc
      121 gcggtggcgg ggttcaacca ggtgatgacc gcgatcgccg ggcacttcgc ggacggagcc
      181 ggtccggcgc ggatggtgga gttcgcggcg caggagtcgg gctacctcgc ggagctcgag
      241 ggggatcgca cggtcgaggc gcaggggcgg atcgagaaca tccaggagct gtcgggggtg
      301 gccgcggagc tcctcgcacg cgaccccgac gcgggcatcg acgtgttcct cgaacaggtg
      361 tccctgatcg gggagcagga cgactacgac gaggaggagt cctcggtcac gctgatgacg
      421 ctgcacatcg ccaaggggct ggagttcccc gcggtgttca tcgtggggct cgaggacggg
      481 atcttccccc actaccggtc gatgaccgac tcggcgcagc tcgaggagga acggcggctg
      541 ctctacgtcg ggatcacgcg ggcgcagaag cgtctctacc tctgccacgc gtggggccgg
      601 acactgttcg gacagacgaa ctacaacccg ccctcgaggt tcctgaacga gctgcccccc
      661 aacctgctcg atgtgcggga ggagggtggg tccgggaggg ggtcgcatcc gcgcgatgct
      721 tcccgaggtg gccgcgcgag ccgggcggag cgcgggtaca cggtcgtcgg gctcccgggg
      781 aagggacagg tcgatgtggc gcccaccggt tcgtggtcac cctcgccacc tccgaaacgc
      841 caggtcccca ccgtcgccga aggagacacc gtcgtccacg agcgctgggg cgaaggggtc
      901 gtggtggcga tcaagggaca cggggacgac acggaggcga ccatcgcgtt ctccgaggcg
      961 ggggagaagc ggctgctgct ctcctacgcg ccgctgacgc gggtgggctg atcctcagcc
     1021 ttcgtgtcac gcggacaggc gctccagcac gttccgggtg atacggcgca cgctcgcatc
     1081 tcctccttcg atgccgtacg tccagtcggt gcatccggcg ttgaacacgg tgcccccgcc
     1141 ggggtggtcg aacgaggcga cgaccgcgtg gttgtgccgg atgcggtgca gctcctcctg
     1201 ccagccgtcc ccgaacagtg cctgggcgac gtgctcgagc tctccaggct cgtcggcgta
     1261 gcgcgacggc tgctcgtcct gtgaccagag cgtcgccggc gccgtcgcca ggatctgcag
     1321 ggtgtcgggc gctccgtcca tgtgcgtggg aaccggcagg ccgtcctcga gggtcagctc
     1381 gcacccatcg acctcgtagc cgacgatcgc gtccgccagc cccagcgcgt ctccgtagcg
     1441 gagatcggtc ccttcgaacg cccagtggtc cgctcgccac accgtgtagg cgcccgaggc
     1501 gcgagggacg ccgagtccgt agcgggagta gccacctcgt gagaacgaca gccccgtcat
     1561 cgccgcttcg aggcggccga ccctccggtc ggcccacgct cccgtcagcg atcgctcctc
     1621 gggcgtcccg agcacgggat cctccgccgc ccggtacttg aagcacgtca tcccacgatg
     1681 acggtcgtcg aaccgcacct gccaccagca cgtgttcccg ctgaagaagg cggcgttgcc
     1741 gcctccggcg aggaacgtct cgacggtgtc gcgcatcccc cacgaccagt actcgtcgtg
     1801 gcccacggac aggaacatgc ggtggccgtc gagcacctcg gggtgccgct cgaggtcctg
     1861 ggagacggcg atgtcgatct cgtagccgga tgcctcggcc cacgcgacga acgggcgttc
     1921 ccacgtccac cagccggcgc cgccactcca gggcgacagg ccgtgctcct ccgcccaccg
     1981 gaagtagccg agtgcctcgc gatccggcac cgcctcggcc ttccggacgg ccggctccgg
     2041 cttgtcgagg aagccccggg cgagcggccg ctcgaacgac acccgcgtgc cgcccgtgta
     2101 cagcgagg
//
LOCUS       DASUYD010000263         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_214645, whole genome shotgun sequence.
ACCESSION   DASUYD010000263 DASUYD010000000
VERSION     DASUYD010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214645"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..2462
                     /locus_tag="VF195_13615"
     CDS             48..2462
                     /locus_tag="VF195_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008741424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX6581887.1"
                     /translation="MASAVLHVGGVYRASEKSVVEQVLGHRPGVITVEANPVGQTANV
                     TFDTAETSVAELRRWVEDCGFHCAGQSVPEHICDPLMEPDPPHMVHATHATVAEPPAP
                     PAERMGHIGHEVSPVAPASEHAEHAMAHEEAMPSPHEMMGHGGAAGMSMDAMVRDMRN
                     RFLVAALLSIPILLWSPIGRDVLNFEVDAPFGLRDDVWLLLLSLPVIFYSSWIFFDGA
                     VRALRARTLDMMVLVAVAIGTGWVYSLVVSLTGGGDTFYEAATVLAAFVLLGHWFEMR
                     ARGGANDAIRTLLDLAPPKATVIRDGEAVEIPTAKVVVGDLMLVRPGDKIGVDGVVEE
                     GQSEVDESVVTGESLPVSKGPGSEVIGATINRNGTLRIRATKVGADTALAQIVKLVQE
                     AQNSKAPGQRLADRAAFWLVLVALIGGAATLAVWLAVGESFSTAMLYAITVVVITCPD
                     ALGLATPTAIMVGTGLGAKRGILFKNAIALETSARIQAVVMDKTGTLTKGEPEVTDVV
                     VDGMGEGGVLRLAAAVERDSEHPLAEAVVKHADSKAIPNVRAENFENIPGHGAIALVD
                     GHRVAVGNARLMDLEGADLGPLEARREDLASTGRTVVCVAVDGEAVGLVGIADAARET
                     SAAAVRALSDSGVEVIMLTGDNEATAKRIADQLGVKTVIAEVLPGDKASRIAELQAAG
                     KKVAMVGDGVNDAPGLAQADLGIAIGAGTDVAIETADVVLMRSDPLDIPTTLEIGRGT
                     LRKMRQNLGWAIGYNSVALPIAAGVFEPLFGLVLRPEIAALSMSGSSLLVAVNALLLK
                     RLRLPR"
     gene            2676..2945
                     /locus_tag="VF195_13620"
     CDS             2676..2945
                     /locus_tag="VF195_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013172767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6581888.1"
                     /translation="MQENQDGILNRLRRIEGQVTGIRKMHEEGRYCIDVLDQISAARA
                     GLEATALLILEGHVNGCVREAIEEGRGEEKAAELLEAVGRFARSV"
     gene            2947..3477
                     /locus_tag="VF195_13625"
     CDS             2947..3477
                     /locus_tag="VF195_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581889.1"
                     /translation="MGRSPVILRVTFSVLIAVGSVAALAAPALAHEGEESVPAITNVQ
                     EAIAILAEHVGSFPPAEVAEHAMDKVGDALESEDSRGVQLALVQQAKDALEAGRNDDA
                     LTMLERSIGACPGAPVIEPQDAPRTPPPLSLPCPSVAHFQTLDRSPVGGVQEPVLITV
                     GAVLVLAGLGLARRIR"
BASE COUNT          583 a         1187 c         1292 g          569 t
ORIGIN      
        1 acgccgctgg tggcggtcat ggccacggtc aacgagatga cgggaagatc gcctccgcgg
       61 tcctccacgt gggaggggtg taccgcgcga gcgagaagtc ggtcgttgag caggtgttgg
      121 ggcaccgtcc gggagtgatc accgtcgagg cgaatcccgt cgggcagacg gccaacgtca
      181 ccttcgatac ggccgagacc tcggtggccg agctccgccg ttgggtcgag gactgcgggt
      241 tccattgcgc gggccagtcg gtgcccgagc acatctgcga ccccctgatg gagccagatc
      301 cgccccatat ggtgcatgcc acccacgcga ccgtcgcaga accccccgcg ccgccggccg
      361 aacgcatggg tcacataggc catgaagtct cgcccgttgc gccagccagt gagcacgcgg
      421 aacatgcgat ggctcacgag gaggcgatgc cgtcgccgca cgagatgatg ggccacggcg
      481 gtgccgccgg gatgtccatg gacgcgatgg tccgcgacat gcgcaaccgg ttcctcgtgg
      541 cggcgctgct ctcgatcccg atcctgctct ggtcaccgat cggccgcgac gtcttgaact
      601 tcgaggtcga cgcaccgttc ggtctccgcg acgacgtctg gctcctcctg ctcagcctcc
      661 cggtgatctt ctattcgtcg tggatcttct tcgacggcgc cgtccgtgct ctgcgggctc
      721 gaacgctcga catgatggtt ctggtggcgg tggcgatcgg aaccggctgg gtgtactcgc
      781 tggtggtctc gctcaccggc ggcggggaca cgttctacga ggcggcgacg gtgctcgcgg
      841 ccttcgtcct cttgggccac tggttcgaga tgcgtgcccg cggtggggcg aacgatgcga
      901 ttcggacgct gctcgacctc gcgccgccga aggcgacagt tatccgcgac ggcgaggccg
      961 tcgagatccc tacggccaag gtcgtggtcg gtgacctgat gctggtgcgc cccggcgaca
     1021 agatcggcgt cgacggggtg gtggaggaag gccagagcga ggtcgacgag tcggtggtca
     1081 ccggcgagag cctccccgta tcgaagggac ccgggtccga ggtgatcgga gccacgatca
     1141 acaggaacgg gacgctccgc atccgagcca ccaaggtcgg ggcggacacg gcgctcgcgc
     1201 agatcgtgaa gctcgtccag gaggcgcaga actccaaggc gccgggacaa cgcctcgccg
     1261 accgcgccgc cttctggctc gtgctcgtgg ccctgatcgg cggtgcggcg acgctcgccg
     1321 tctggctggc agtcggagag tcgttcagca cggcgatgct gtacgcgatc acggtcgtcg
     1381 tgatcacctg ccccgacgcg ctcggcctgg cgacgccgac cgcgatcatg gttgggaccg
     1441 gcctcggcgc gaagcgcggc atcctgttca agaacgcgat cgccctcgag acctcggcgc
     1501 ggatccaagc tgtggtgatg gacaagaccg gcaccctcac caagggggag ccggaggtga
     1561 ccgacgtggt cgtcgacgga atgggggagg gcggggtcct ccggctggcg gcggccgtcg
     1621 agcgcgattc ggagcatccc ctcgcggagg ccgtggtcaa acacgccgac tcgaaggcga
     1681 tcccgaacgt ccgcgcggag aacttcgaga acatcccggg gcacggcgcg atcgccctcg
     1741 tggatggcca tcgcgtcgcg gtggggaatg cacgcctgat ggacctggag ggggccgacc
     1801 tcggtccgct cgaggctcgc cgagaggacc tggcctcgac cgggcggacc gtcgtctgcg
     1861 tagcggttga cggagaggcc gtggggctcg tcggcatcgc cgacgcagcg cgtgagacgt
     1921 ccgcggccgc cgtgcgtgcc ttgagcgatt ctggcgtcga ggtgatcatg ctgactggcg
     1981 acaacgaggc cacggcgaag aggatcgccg accagctcgg cgtcaagacg gtgatcgccg
     2041 aggtgcttcc gggcgacaag gcgagccgga tcgcggagct gcaagcggcc gggaagaagg
     2101 tcgccatggt cggcgacgga gtgaacgacg cgccagggct cgcccaggcc gacctcggga
     2161 tcgccatcgg ggccggcacg gatgtcgcaa tcgagacggc cgacgtggtc ctcatgcgct
     2221 ccgatccgct cgacatcccg acaacccttg agatcggccg cggaacgctc cggaagatgc
     2281 gccagaacct gggatgggcg atcggctaca actcggttgc gctaccgatt gccgccgggg
     2341 tgttcgagcc gctcttcgga ctcgtgcttc ggcccgagat cgcggccttg tcgatgtccg
     2401 gctcgagcct tctcgtggcg gtcaacgcgc tcctgctgaa gcggctccgc ttgccgagat
     2461 agaccaccta ccgctgggca ggcacgcgcg aaccaggtga tggaggaggc caacctcgtc
     2521 gtcctagcca ccctgctcct gcgctgagcg gcggtccttc ggccccccgg gagaggggca
     2581 tccggcccgt gtccggccgt ggctgcgtga cgatgctatg cccaaccggg gtaccgcaat
     2641 cgagccgggc gagtcgtcat atggaagggg tgaccatgca agagaaccag gacggcatct
     2701 tgaaccggct ccgccggatc gaggggcagg tcaccggcat ccgcaagatg cacgaggaag
     2761 ggcggtactg catcgacgtg ctcgatcaga tctcggccgc ccgggccggg ctcgaggcca
     2821 ccgctctcct gatcctcgag gggcacgtga acgggtgcgt ccgagaggcg atcgaggaag
     2881 gcagggggga agagaaggcc gccgagctcc tggaagcggt cggcaggttc gcgaggagcg
     2941 tgtgagatgg ggcggtctcc ggtcatcctc cgagtcactt tcagcgtcct catcgcggtc
     3001 ggatcggttg cggctctagc agcgcccgca ctggcccatg agggagagga atctgtaccg
     3061 gccatcacca acgtccagga agcgatcgcc atcctcgccg aacacgtcgg atccttcccc
     3121 cccgccgagg tcgcggagca cgccatggac aaggtgggcg acgccctgga gtcggaggac
     3181 tcccgcgggg tgcagctggc cctggtgcag caggctaagg atgctttgga ggccggtcgg
     3241 aacgatgacg ctctcaccat gctcgagcgg tcgatcggcg cgtgtcccgg cgctccggtg
     3301 atcgagccgc aggacgcgcc acggacgcct cctccactct ccttgccgtg cccatcggtc
     3361 gctcatttcc agacccttga ccgctctccg gtcggcggcg tgcaggaacc ggtgctgatc
     3421 accgtcggag ccgtgctggt cctggctggg ctcggcctcg cgaggaggat ccgatgaacg
     3481 acgaggccac ctccgcgccc tcgacgttga cggagcctgc tggccccacg acccgtcggc
     3541 gattctggga cgcactggac gaacggctcg gcttgaaggg gcttcagtac ccgatcccag
     3601 aacacgccaa caagctcgcg tactcgctcg g
//
LOCUS       DASUYD010000264         8637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_875261, whole genome shotgun sequence.
ACCESSION   DASUYD010000264 DASUYD010000000
VERSION     DASUYD010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875261"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VF195_13630"
     CDS             complement(<1..506)
                     /locus_tag="VF195_13630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581890.1"
                     /translation="MESYRVATLARPAMRSLLRFERQPPGTAITRSVLVAGGALAAGA
                     GFLVVAREARLAAWAAGASGALLLLGGHRANHGAGGPTDRMLDDLLDRVWDGAVLSAI
                     AWTAHDGSASLAVAALAAVSLSFVAGYIRARGAALGYSVEERHVTRGARYVLVVAFLL
                     FGWTWAVWT"
     gene            569..2659
                     /gene="fusA"
                     /locus_tag="VF195_13635"
     CDS             569..2659
                     /gene="fusA"
                     /locus_tag="VF195_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX6581891.1"
                     /translation="MKTYDAKDIRNVLLVGHGGSGKTTLLEAMLFASGAITRMGTVED
                     GNTVSDHDPDEQRRQISVSLSTAPIEWNDAKINVLDAPGTADFVGDVRSAVRAADAVI
                     IVVSAVDGVEVQTEFAWELAVQENLPRAVFVNKLDRERADFQATLDQLVSSFGNQVAP
                     FELPIGAESQFSGIADLLHEKADLYPSGPKAEESEWPAEIHQIADPAREKLIEAVAES
                     DDALIEQYLEEGTLPEAVITQGAKDGFTNARLAPVFVGSASKAIGIDRLLDFIVEEFP
                     SPLDRAPLQVIAKGGEEKERACEPDGPLSAFVFKTVSDPFVGHITMFRVFSGKVRPDS
                     AVHNATQGTDERIGQLFALKGKEHETVSEVPAGDIGAVAKLPHTHTGDTFATKDDPVQ
                     VAPVELPEPLLAYAIAPKTKGEEDRLATGLARLREEDPTFRVARNDETHETVIYGMGE
                     AHLGVQMERLKAKFGVEVEAKPAKIAYRETIRARAKAQGRHVKQSGGHGQYAVCEIEV
                     EPLPRGEGFVYEDKIFGGAIPQQFIPSIEKGIVKTMSEGVLSGNPMVDIKVTLVDGKF
                     HSVDSSDMAFQIAGSLALKEVVEQAGVALLEPIVRLQVTVPESFTGDIMGDLNAKRGK
                     IQGMDQLGGGKQVINAMVPQGEVARYVIDLRSMTGGRGAFSMMFSHYEEMPQHLAQKV
                     IDEYQHAREEAHKK"
     gene            complement(2663..3502)
                     /locus_tag="VF195_13640"
     CDS             complement(2663..3502)
                     /locus_tag="VF195_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581892.1"
                     /translation="MTDVKTRLQEAGRLVNPPDRPFERLVERRARAHRRDRVISLVVA
                     LAVAGGVVGGGLFLLNGIDDPAKDAGAGWRPTRRLALLPGEYFYLRITSGEAEDGHIR
                     DMETWWAQDGSGEARNRSTRQDKYPYPPTGVYGEDEFPEPYDLAWLSTDTEVLAAQLR
                     EATFRGDFGLPETPYASPEVRAALFEVASGSAGVTVIEDTHDPAGRLAIALETSERDG
                     AFTATWRTYFDPGTHQALAWTFESSRGGSAWILLESAIVDASGERPDVDEWLVPPVEE
                     ALA"
     gene            complement(3495..4028)
                     /locus_tag="VF195_13645"
     CDS             complement(3495..4028)
                     /locus_tag="VF195_13645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX6581893.1"
                     /translation="MDMTAGPAVDAIEHDVSAEAGRDFESFFRLEHERLFRAMWLLTR
                     HRQEAEEVTQEAFLRLWERWDRFVDAPDSDAYLYRTALNVWRSRLRRVAIAARKVVHH
                     RRREDEMESVVARDVVVRTLASLPGRQRAAIVLMDVLDLSSERAGEVLGIRPVTVRVL
                     AARARVTLANEMGAGDD"
     gene            complement(4137..4949)
                     /locus_tag="VF195_13650"
     CDS             complement(4137..4949)
                     /locus_tag="VF195_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX6581894.1"
                     /translation="MPKLELPDGAHLNYEIAGDGPTVTFNHPGLWDMRTWDREFASFG
                     QAGYRVLRYDVRGYGGSSRPEPGVPYSHARDLEALLALEEVTQTAIVGCSMGGGIDID
                     YALEHPERVWSLVLVAPGLGGFESTEEEEDWWAERDAPIEAAIDAGDLERAQDLLLEI
                     WAPLGTHDERGALIKQIARDNIHELTMDESGAEQLDPPAAHRLAEIDVPTLILEAGYD
                     PPDMRRLCDFLGIEIMGSRKVVVEDADHVVNMRRPDRFEELVLPFLADAAPR"
     gene            complement(4972..5742)
                     /locus_tag="VF195_13655"
     CDS             complement(4972..5742)
                     /locus_tag="VF195_13655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581895.1"
                     /translation="MIAVGIWLLAVGLADLVAGLSGEPKDGARTIGAIVTGVVAPAIL
                     CGIAGADVDTLVVLTLLTGATTAVWIVIRRDAPRMSSVRALVGLLFLLTAFAIVSAIG
                     SEPVSAFATGSLLERAIGRSPFSSLASSSPEQVLFVLGVLAMLFATSNAIVRLVLTAM
                     GTRFSNAEQRLRGGRVIGPLERVLIFGLGLAGEATAAALVIGAKGLLRYPELNGLRGE
                     SGAAGPSARTIDVVTEYLLIGSLTSWLLALFPLALVPA"
     gene            complement(5739..6401)
                     /locus_tag="VF195_13660"
     CDS             complement(5739..6401)
                     /locus_tag="VF195_13660"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEX6581896.1"
                     /translation="MEPAGAERLVAVLGDVVASRRHPDRERRLTAIGGALDLANRRVG
                     ATQPLTPTIGDEFQGLYGDLVSALDATLVVRLALLGEVDVRFGIGSGELLGYEASRAP
                     FEQDGPAWWAAREAVDMVRDLEQRRERSRGLRTRWIDGGAEGASPVGAVNAFLHCRDE
                     LVTAMDPRDADILLGLLVDEPLTSIAKRLGVSVSAVSQRAIRGGLYAIRQAHQELREA
                     VA"
     gene            complement(6495..6953)
                     /locus_tag="VF195_13665"
     CDS             complement(6495..6953)
                     /locus_tag="VF195_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012571398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX6581897.1"
                     /translation="MEYIQAVKDDEDEGCVLCRIRDGEESERVLARSELGYVVLNKYP
                     FNPGHVMVVPNRHDGSFEALADDELLDLQRLIRRSVSALREEMAPHGFNVGANIGRIA
                     GAGMPDHLHWHVVPRWSGDTNFMPVVGQTRVMPELLEETAARLAPRFVVD"
     gene            complement(7063..>8637)
                     /gene="thrS"
                     /locus_tag="VF195_13670"
     CDS             complement(7063..>8637)
                     /gene="thrS"
                     /locus_tag="VF195_13670"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX6581898.1"
                     /translation="AIVEDDQPFLREEVTREDALARLEGQPFKREIIESIGSAQGEVR
                     TGDVVSLYRNDGWVDLCEGPHVPSTGRLGAFTLTSTAGAYWRGLETNPMLTRIYGTAW
                     ATREDLEAHLHRLEEAEKRDHRRLGRELDLFSSPDELGPGLWIWHPRGGVFRKQLEDW
                     VRDLHVERGYDLIVTPHIARSVLWETSGHLEKYRENMYPPMEADTGDYYVKPMNCPFH
                     VLVYKSQVRSYRDLPMRLSELGTIYRHEKAGTLHGLLRIRGGTQDDSHIICTPEQVID
                     EILAVFDLTLEIHRTFGFTDPVVELSTLPVAQSIVDAETAERATEALTLALEESGLEY
                     RVAEGEGAFYGPKVDFHFRDALGRLWQLTTVQCDFALPERFDMEYTGDDNQRHRPVMI
                     HRAILGTLERFSAVLIEHYGGAFPLWLAPEQLRLVPVADRHVPNCMELAVTARASGLR
                     VHVDETKETVGKKIRAAQLMKSPYVVVVGDRDLDAGTFTVRDRSGEEHQGVPFDRIVS
                     TLAEEARTRALGQSAF"
BASE COUNT         1483 a         2999 c         2845 g         1310 t
ORIGIN      
        1 gtccagacgg cccacgtcca cccgaacaag aggaacgcga ccacgaggac gtaccgagcg
       61 ccacgcgtga cgtgccgctc ctccaccgag tagccgagcg cggccccgcg cgctcggatg
      121 tacccggcca cgaacgacag gctgacggcg gcgagggccg ccacggccag cgacgcgctg
      181 ccgtcgtgtg cggtccacgc gatcgcggac agcaccgccc cgtcccacac gcgatcgagg
      241 agatcgtcca gcatccgatc ggtgggtccg cccgcgccgt ggttggctcg atgccccccg
      301 agcaggagga gcgcgcccga ggcgccggcc gcccacgccg cgaggcgcgc ctcccgggcg
      361 accacaagga atcccgcccc ggccgcgagc gcaccgccgg caacgaggac cgagcgcgtg
      421 atcgcggtgc cgggtggctg gcgttcgaac cgcagcaagc tccgcatcgc aggtcgggcg
      481 agcgtagcaa ctctgtagga ttccacgacc ctgacccatc acccggtgag agcgtcagcc
      541 tcagccctcg tccacgaagg agccccggtt gaagacctac gacgcgaagg acatccgcaa
      601 cgtcttgttg gtgggacacg gtggctccgg caagacgacg ctgctggaag cgatgctctt
      661 cgcgtccggt gcgatcaccc gcatgggcac ggtcgaggac gggaacaccg tgtcggacca
      721 cgaccccgac gagcagcgac gccagatctc cgtgtcgctc tccacggcac cgatcgagtg
      781 gaacgacgcc aagatcaacg tcctcgacgc gcccggcacg gcggacttcg tcggggacgt
      841 gcgctcggcg gtccgcgcgg cggacgccgt gatcatcgtt gtctccgcgg tggacggcgt
      901 cgaggtccag accgagttcg cgtgggagct cgcggtccag gagaacctgc ctcgcgcggt
      961 cttcgtgaac aagctggatc gtgagcgagc ggacttccag gcgacgctcg accagctggt
     1021 gtcttccttc gggaaccagg tggcgccgtt cgagctcccg atcggcgcgg agagccagtt
     1081 cagcggcatc gccgacctgc ttcatgagaa ggcggacctg tacccgtccg gaccgaaggc
     1141 ggaggagtcc gagtggcccg ccgagatcca ccagatcgcg gatcccgcgc gggagaagct
     1201 gatcgaagcg gtcgccgagt ccgacgacgc attgatcgag cagtacctcg aggagggcac
     1261 gctccccgaa gccgtcatca cacagggggc gaaggacggc ttcacgaacg ctcgactcgc
     1321 tcccgtcttc gtcggctcgg cctctaaggc gatcgggatc gatcggctgc tggacttcat
     1381 cgtcgaagag ttcccctccc ccctcgatcg cgcgccgctg caggtgatcg cgaagggcgg
     1441 cgaggagaag gagcgggcat gcgaacccga tggacccctg agcgccttcg tgttcaagac
     1501 cgtgtcggac cccttcgtcg ggcacatcac gatgttccgg gtcttcagcg gcaaagttcg
     1561 ccccgacagc gccgtgcaca acgcgaccca gggaaccgac gagcggatcg gacagctgtt
     1621 cgccctcaag gggaaggaac acgagaccgt gtccgaggtg ccggcaggcg acatcggcgc
     1681 ggtcgcgaag ctcccccaca cccacaccgg ggacacgttc gccacgaagg acgatccggt
     1741 gcaggtcgcg ccggtggagc tcccggagcc gctcctcgct tacgcgatcg cgccgaagac
     1801 caagggtgag gaggaccggc tcgccaccgg tctggcgcgc ctccgcgagg aggacccgac
     1861 gttccgggtg gcccgcaacg acgagacgca cgagacggtg atctacggga tgggcgaggc
     1921 acatctcggc gtgcagatgg agcgcctcaa agcgaagttc ggcgtcgagg tggaggcgaa
     1981 gcccgcgaag atcgcatacc gagagaccat ccgggccagg gccaaggccc agggacggca
     2041 cgtgaagcag agcggaggcc acggacagta cgcggtgtgc gagatcgagg tcgagccgct
     2101 cccgcgtggg gaaggcttcg tctacgagga caagatcttc ggaggagcca tcccccagca
     2161 gttcatcccg tcgatcgaga agggcatcgt caagacgatg tccgagggcg tcctgtcagg
     2221 gaacccgatg gtcgacatca aggtcacgct cgtcgacggg aagttccact ccgtggactc
     2281 ctccgacatg gcgttccaga tcgcaggctc actcgcgttg aaggaggtcg tcgagcaagc
     2341 cggcgtcgcg ctcctcgagc cgatcgtccg gctccaggtg accgtcccgg agtccttcac
     2401 cggcgacatc atgggcgacc tcaacgcgaa gcgcgggaag atccaaggca tggaccagct
     2461 gggcggcggc aagcaggtga tcaacgcgat ggtcccccag ggcgaggtcg cccgctacgt
     2521 gatcgacctg cggtcgatga ccggtggccg cggagcgttc tcgatgatgt tctcgcacta
     2581 cgaggagatg ccgcagcatc tcgcgcagaa ggtcatcgac gagtaccagc acgcccgcga
     2641 ggaagcccac aagaagtagg agctacgcga gcgcctcctc gaccggaggg acgagccact
     2701 cgtccacgtc cggtcgttca ccggatgcgt cgacgatcgc ggactcgagg aggatccatg
     2761 ccgagccgcc acgtgatgac tcgaacgtcc atgccagggc ctgatgcgtt cccgggtcga
     2821 aatacgtccg ccaggtcgcc gtgaacgcac cgtcgcgctc ggacgtctcc agtgcgatcg
     2881 cgagccgacc ggccgggtcg tgagtgtcct cgatcaccgt cacgccggca gacccgctcg
     2941 cgacctcgaa gagggcagcc ctgacttccg ggctcgcgta cggagtctcg gggaggccga
     3001 agtcaccccg gaaagtcgct tcgcgcagct gcgccgcgag aacctcggta tccgtcgaca
     3061 gccacgccag gtcatacggc tcggggaact cgtcctcgcc gtagacaccg gtgggcgggt
     3121 acggatactt atcctgcctg gtgcttcggt tccgcgcctc gccagacccg tcctgcgccc
     3181 accatgtctc catgtcgcgg atgtgcccgt cctcggcctc gccactcgtg atccggaggt
     3241 agaagtactc cccaggcaag agcgcgagcc ggcgcgtcgg tcgccaaccc gcccccgcat
     3301 ccttcgcagg gtcgtcgatc ccattcagca ggaacagacc cccacccaca acgccgccag
     3361 cgaccgcgag tgcgacgacg agcgagatga cccggtcgcg tcggtgtgct cgggcgcgcc
     3421 gctccaccaa ccgttcgaac ggacgatccg gcgggttcac gagccgaccg gcctcttgga
     3481 gtcgtgtctt cacgtcagtc atcgcctgct cccatctcgt tcgccaacgt gacccgagcc
     3541 ctcgctgcga gcaccctgac cgtcacggga cggatcccaa gaacctcgcc cgcccgttca
     3601 gacgagaggt cgagcacgtc catcaagacg atggccgctc gctggcgtcc cggaagcgaa
     3661 gcgagggtcc gcacgaccac gtccctcgcc acgacgctct ccatctcgtc ctcgcgcctc
     3721 ctgtgatgga ccaccttgcg ggctgcgatc gccacccggc ggagtcggct ccgccatacg
     3781 ttgagggcag tgcgatagag gtaggcgtcg gaatcgggcg cgtcgacaaa tcggtcccac
     3841 cgttcccata ggcgtaggaa tgcctcttgc gtcacttcct cagcctcctg tcggtggcgt
     3901 gtaaggagcc acatcgcccg gaacagccgc tcgtgttcga gcctgaagaa cgattcgaag
     3961 tcgcgcccgg cctcggcgga gacgtcgtgc tcgatcgcgt ccacggcggg cccggcggtc
     4021 atgtccatcc gtgccatgta cgcgcaggat gacctgcttc gtttacgagc ggaagaagat
     4081 cgaacgggac cggttgtgct gcctgctcag agggaagccc tcagcctccg gcttcatcac
     4141 cgaggcgcgg catcggcgag gaatgggagc acgagttcct cgaaccggtc gggtcggcgc
     4201 atgttcacca cgtggtcggc atcttccacc acgaccttgc gcgagcccat gatctcgata
     4261 ccgaggaagt cgcacaggcg gcgcatgtcc ggtgggtcgt acccggcttc gaggatcagt
     4321 gtggggacat cgatctccgc cagccgatgg gccgcgggag gatcgagctg ctccgcgccg
     4381 ctctcgtcca tcgtcagctc gtggatgttg tcgcgcgcga tctgcttgat cagggcaccg
     4441 cgctcgtcgt gggtcccgag tggcgcccag atctcgagca ggaggtcctg cgcgcgctcg
     4501 aggtcgcccg catcgatcgc ggcctcgatc ggcgcgtcac gttccgccca ccagtcctct
     4561 tcctcctcgg tcgactcgaa gccgcccagg ccgggcgcga cgagcacgag actccatacc
     4621 cgttcgggat gctccagcgc gtagtcgata tcgatgcctc cgcccatcga gcagccgacg
     4681 atcgcggtct gcgtgacctc ctcgagagcg aggagcgctt cgaggtctcg ggcgtgcgag
     4741 tagggaacgc ccggctcggg ccggctcgag cctccgtacc cacggacgtc gtagcggagc
     4801 acccgatagc ccgcttgccc gaaggacgcg aactcccgat cccacgtcct catatcccag
     4861 agcccgggat ggttgaacgt taccgtcgga ccgtcgcccg cgatctcata gttcaggtgc
     4921 gcgccgtccg gcagctcgag cttcggcatc gtccgagcct agcctcagtc gctaggcggg
     4981 gaccaacgcg agcgggaaga gggcgaggag ccagctcgtg agcgatccga tcaggaggta
     5041 ctcggtgacg acgtcgatcg tccgcgcgga cggtccggcg gcgccggatt cgccgcggag
     5101 gccgttcagc tccggatatc gcaggagtcc cttggcgccg atcacgaggg ctgccgcggt
     5161 ggcctctccg gcgagcccga ggccgaagat cagcacacgt tcgagcggcc cgatcacccg
     5221 ccccccgcgg agtcgttgct cggcgttcga gaacctcgtt cccatcgcgg tcagcaccag
     5281 acggacgatc gcgttgctcg tggcgaagag catcgcgagc acaccgagga cgaacaagac
     5341 ctgctcgggt gaggacgaag cgagcgatga gaacggcgac cggccgatgg cgcgctcgag
     5401 gagcgagccg gtcgcgaacg ccgagacggg ctcgctcccg atggccgaga cgatcgcgaa
     5461 ggccgtcaga aggaacagca gcccgacgag tgcccggacc gagctcatgc gaggtgcgtc
     5521 ccggcggatg acgatccaca cggccgtggt ggcgccggtc aggagggtca gtaccacgag
     5581 cgtgtcgaca tcggcgccgg cgatcccgca caggatcgcc ggtgcgacga caccggtcac
     5641 gatcgctccg atcgtcctcg cgccgtcctt cggctcaccc gagagtccgg cgacgaggtc
     5701 cgccaaaccc accgccagca gccagatccc caccgcgatc acgcgacggc ctctcgcagc
     5761 tcctggtgcg cctgtcggat ggcgtacaga ccgccgcgga tcgcgcgttg ggagacggcc
     5821 gagacgctca cgcccaggcg cttcgcgatc gaggtcagcg gctcgtcgac cagcaatccg
     5881 agcaggatgt cggcgtcccg cggatccatc gccgtgacca gctcgtcccg gcagtgcagg
     5941 aacgcattga cggctccgac cggtgacgca ccctcggccc ccccgtcgat ccaacgggtc
     6001 cgcagcccgc gcgaccgttc ccgccgctgc tccagatcac gaaccatgtc gaccgcctcc
     6061 cgcgccgccc accaagccgg accgtcttgc tcgaacggag cccgcgacgc ctcgtacccc
     6121 aggagctcgc ccgacccgat cccgaaccgg acgtcgacct ccccgaggag ggcgagccgc
     6181 acgaccagcg tcgcatcgag cgccgacacg aggtctccgt agaggccctg gaactcatcg
     6241 ccgatcgtcg gtgtcagcgg ctgcgtcgcc ccgaccctcc ggttcgccag gtccaacgcg
     6301 ccgccgatcg ccgtcaaccg tcgctcgcga tccggatgtc gccgcgaagc gacgacgtca
     6361 ccgagcacgg ccacgagccg ttcggctcca gccggttcca tgatgtgaag tgtatgcctt
     6421 cagtttgaag aagtaaagat gtgtgcttca tatcgtttga gtgaagatat gagctgcaga
     6481 ggtcccccga cgactcaatc gaccacgaac cggggagcca atcgagcggc ggtctcctcc
     6541 agaagttccg gcatcacccg cgtctgcccg acgaccggca tgaagttcgt gtccccgctc
     6601 caacgaggga ccacgtgcca atggagatgg tccggcatcc ccgccccggc gatccgaccg
     6661 atgttcgcgc ccacgttgaa gccgtgggga gccatctcct cgcggagcgc cgagacggac
     6721 ctccggatga ggcgttgcag atcgaggagc tcatcgtcgg cgagcgcttc gaagctgcca
     6781 tcgtgacggt tcggcacgac catcacgtga cccgggttga acgggtactt gttgaggacg
     6841 acgtagccga gctccgacct cgcgaggaca cgctcggact cctccccgtc gcggatccga
     6901 cagagcacgc acccttcgtc ctcgtcgtcc ttgacggcct ggatgtactc catgcgccaa
     6961 ggactccaca ggtgccccat ctatcgcgcc gatccttggc cggcgcgacc ctcaacggcg
     7021 cgaccctcaa gggcgcgacc ctcgccggcg ccgcgcatcc gtctagaagg cgctctgacc
     7081 gagcgcacgc gtccgtgcct cctcggcgag ggtcgacacg atccggtcga acggtacgcc
     7141 ctgatgctcc tcccccgatc ggtcccggac ggtgaacgtc ccggcatcca gatctcgatc
     7201 gcccacgacc accacgtacg gcgacttcat cagctgggcc gcacggatct tcttgcccac
     7261 cgtctctttc gtctcgtcga cgtgcacccg gaggccggat gctcgcgccg tgacggcgag
     7321 ctccatgcag ttgggaacgt gccggtcggc caccgggacc aggcggagct gctccggcgc
     7381 gagccacagc gggaacgcgc caccgtagtg ctcgatcagg accgcgctga accgctccaa
     7441 ggtcccgagg atcgcccggt ggatcatcac cggccggtgc cgctggttgt cgtcgcccgt
     7501 gtactccatg tcgaagcgct cgggtagggc gaagtcgcac tggacggtcg tgagctgcca
     7561 caagcgtccg agcgcgtcgc ggaagtggaa gtccaccttc ggtccgtaga acgcgccctc
     7621 gccctcggcg acgcggtact cgagaccgct ctcctcgagc gccagcgtca gcgcctcggt
     7681 ggcgcgttcg gccgtttcgg cgtccacgat cgattgagcg acgggcagcg tcgagagctc
     7741 gaccacgggg tcggtgaagc cgaaggtgcg gtggatctcg agcgtgaggt cgaagaccgc
     7801 gaggatctcg tcgatcacct gctcgggtgt gcagatgatg tgcgagtcgt cctgggttcc
     7861 gccgcggatc cggagcagcc cgtggagcgt gccggccttc tcgtggcggt agatcgtgcc
     7921 gagctccgat agccgcatcg gaaggtcgcg gtagctccgc acctgcgact tgtagacgag
     7981 cacgtggaac gggcagttca tcggcttgac gtagtagtcg ccggtgtccg cctccatcgg
     8041 gggatacatg ttctcccggt acttctcgag gtggccgctc gtctcccaca gcacggaccg
     8101 ggcgatgtgc ggcgtgacga tgaggtcgta tccacgctcg acgtggagat cccggaccca
     8161 gtcctcgagc tgcttccgga agacgccgcc gcggggatgc cagatccaca ggcccggacc
     8221 gagctcgtcg ggactcgaga acagatcgag ctcgcgaccg agacgccgat gatcacgttt
     8281 ctcggcttcc tccagccggt gaaggtgtgc ctcgaggtcc tctcgcgtcg cccaggccgt
     8341 cccgtagatc cgggtgagca tcgggttggt ctcgagccct cgccagtagg cgccggcggt
     8401 cgaggtcagc gtgaacgcgc cgaggcggcc tgtcgagggg acgtggggac cctcgcacag
     8461 gtccacccac ccgtcgttgc ggtagagcga cacgacatcg cccgtccgta cctcgccttg
     8521 ggccgaaccg atcgactcga tgatctcgcg cttgaacggc tggccctcga gccgggcgag
     8581 cgcgtcctcg cgcgtgacct cttcgcggag gaacggttgg tcgtcctcca cgatcgc
//
LOCUS       DASUYD010000265         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1762457, whole genome shotgun sequence.
ACCESSION   DASUYD010000265 DASUYD010000000
VERSION     DASUYD010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1762457"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..694
                     /locus_tag="VF195_13675"
     CDS             5..694
                     /locus_tag="VF195_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017544158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX6581899.1"
                     /translation="MRTRILLVEDEGTIAEPLAESLGRDGFEAEVAATLADAREAIRR
                     EPPDLVLLDVMLPDGDGRDLAREIRQGSDVPIVILTARGEEIDRVLGLELGADDYVVK
                     PFSSRELTARIRAVMRRGRAREQRSPLEIGALRLDPASRTVTKDGDPIVLAAREFDLL
                     HLLMANAGEVLRRERIMDEVWDPHWFGPTKTLDVHISWLRKKIEDDASHPTYIITIRG
                     VGFRFRPVEEP"
     gene            755..2074
                     /locus_tag="VF195_13680"
     CDS             755..2074
                     /locus_tag="VF195_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581900.1"
                     /translation="MILALGIPLTVNLQRRATTELENRAVIQAQGIAAQIGAENIDDP
                     ERLDRIVTQAAVQLGGRVIAVDDQGILVADSDGPESLGASYATPGRPEIRNALAGTPF
                     SQIRDSFDLGHDIMATAVPIQDQQRLLGAVRITRSVQQVNDNVRNVTLGLVAIGVAAL
                     FSGMLLAFGLAGSLSRPLTRLATAAKRLGTGDLSARAGEVGGGTEVEELGHSFDEMAD
                     RLERSVRAQREFVANASHQLRTPLTAMKLRLEGAIADAPNDELKARLEAADREVDRLS
                     GIVDRLLVTAREIEEGRSAQVDLREALDRAVERWNERAGDRDSTVVSRGDGGSARVDP
                     TDLDQILDNLLDNATAYAPGEVTIESGGSDGRMFVAVQDQGPGITSDDLARVTERFYR
                     GRGAPSGGSGLGLAIARQLAEKWGGTLSVESIGGEGTRVEVRLDEAP"
     gene            2116..2523
                     /locus_tag="VF195_13685"
     CDS             2116..2523
                     /locus_tag="VF195_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581901.1"
                     /translation="MNRKTLRAIALGLGGLAVALLLTVGAFAIAGQEISQPAGVPMFT
                     TTPSPSPSDADDAGRSPEPDRTESPSPTDDHGGDSAGSMNSGDSSGSGGSENSGDGDS
                     SGSGSDNSGSGSDSSGSGSGSGSGSGENEHEDD"
     gene            2621..3061
                     /locus_tag="VF195_13690"
     CDS             2621..3061
                     /locus_tag="VF195_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581902.1"
                     /translation="MRKVLRVGAIVIAFALTAWIGVVALAQGSSTPTPSPSPSPTTTT
                     TTTTTATATASPSPSGVDDVKGNCDEAEHANDPECQGVTPVAEDDDVNDDQDEDVNDD
                     HGDDGGDDDDEGEDEGDDHEGGNSGPGSPNSGRGSPNSGPGSAS"
BASE COUNT          547 a         1097 c         1104 g          475 t
ORIGIN      
        1 agccatgagg acgcggatcc tactggtcga ggacgaaggg accatcgcgg aaccactggc
       61 ggaaagcctc gggcgagacg ggttcgaagc cgaagtggcg gcaacgctcg cagatgcccg
      121 cgaggccatc cgtcgtgagc cgccggacct cgtcctgctc gacgtgatgt tgccggacgg
      181 agacggacgt gatctcgctc gcgagatccg tcagggatcg gatgtcccga tcgtgatact
      241 caccgcgagg ggggaggaga tcgatcgcgt cctcgggctc gagctgggcg ccgacgacta
      301 tgtcgtcaaa cccttctcgt cgcgggagct cacggctcgg atccgggccg tcatgcgccg
      361 gggccgcgcg cgggaacagc ggagcccgct cgagatcgga gccctccgtc tcgacccggc
      421 gtcgcggacg gtcacgaagg acggggatcc gatcgtgctc gcggcccgcg agttcgatct
      481 cctgcatctg ctgatggcga acgccggcga ggtccttcgt cgagagcgga tcatggacga
      541 ggtctgggac ccgcattggt tcggccccac caagaccctt gacgtccaca tctcgtggct
      601 ccgcaagaag atcgaggacg acgcctcgca tccgacgtac atcatcacga tccgcggggt
      661 cgggttccga ttccgcccgg tcgaagaacc ctaggagtcg atcacgaagg ttcgcacgag
      721 gctcgtcctc gccttcgcct acatcctcgt ggtggtgatc ctcgcactcg gtatcccgct
      781 cacggtcaac ctccaacggc gcgcgacgac cgagctcgag aaccgtgccg tgatccaagc
      841 gcaggggatc gcggcgcaga tcggggccga gaacatcgac gaccccgagc gcctcgatcg
      901 gatcgtcacc caagcagccg tacagctcgg cggtcgggtg atcgccgtgg acgaccaggg
      961 gatcctcgtc gcggattcgg acgggccgga aagcctcggc gcctcctacg ccaccccggg
     1021 tcggcccgag atccgcaacg cgcttgcagg gacgccgttc tcgcagatcc gcgacagctt
     1081 cgacctcggc cacgacatca tggcgaccgc ggtcccgatc caggaccagc agcggctcct
     1141 cggagcggtc cggatcacgc ggagcgtgca acaggtgaac gacaacgttc gcaacgtgac
     1201 cctcggcctc gtcgcgatcg gggtcgcagc gctgttctcc gggatgctgc tggcgttcgg
     1261 gctcgccggt tccctctcgc gtccgctcac acgcttggcc acggcggcca agcgactcgg
     1321 cacgggcgat ctctcggctc gagccggcga ggtgggcggc ggcaccgagg tggaggagct
     1381 cgggcactcc ttcgacgaga tggccgaccg tctcgagcgc tcggtccggg cccagcgtga
     1441 gttcgtcgcc aacgcctccc accagctccg aacgcctctt acggcgatga agctccggct
     1501 cgagggcgcc atcgcggacg cgccgaacga cgagctcaag gcgcggctcg aagctgcgga
     1561 ccgggaggtc gaccgcctct ccggcatcgt cgaccgtctg ctggtcacgg cgcgggagat
     1621 cgaagaaggc agatcggccc aggtcgatct ccgggaggcg ttggaccgag ccgtggaacg
     1681 ctggaacgag cgcgccggcg accgcgactc cacggtcgtg tcccgaggcg acggtggatc
     1741 agcccgcgtc gacccgaccg acctggatca gatcctcgac aacctgctcg acaacgcgac
     1801 tgcatacgcc cccggcgagg tgaccatcga gtcgggcggc tcggacggcc ggatgttcgt
     1861 cgccgtgcag gaccaggggc ccgggatcac ctcggacgac ctcgcccgcg tgaccgaacg
     1921 gttctatcgg gggcgcggcg ctccttccgg cggttcggga cttgggctcg cgatcgcacg
     1981 ccagctcgcc gagaaatggg gcggcacctt gagcgtggag agcatcggcg gagagggaac
     2041 ccgggtcgag gttcgccttg acgaagcgcc ctgaccttta cctgccctta gcccccgggg
     2101 ccgtaggctc gaaccatgaa ccggaagacc ctgagggcga tcgcgctggg tctcggcggg
     2161 ctcgccgtcg cgctcctgct cactgtgggc gcgttcgcca tcgcgggtca ggagatctcc
     2221 cagccggcgg gcgtccccat gttcacgacg acgccgtctc cgagtcccag cgacgcagat
     2281 gacgccggtc gttccccaga acccgaccgc accgagagcc cttcgccgac cgacgatcac
     2341 ggcggtgact cggctgggtc catgaactcg ggtgactcga gcggctcagg cggctctgag
     2401 aactccggcg acggggactc ctccggctcg ggatccgaca actcgggcag cggctccgat
     2461 tcctcgggct ccggttccgg ctccggctcc ggctcgggcg agaacgagca cgaagacgac
     2521 tgaccccgga tccttatcca ccgcttagca cctcgcttcc gcggacgtaa ccgacccctt
     2581 tctaaagtgg cgatcggtaa ccgttcgaga ggaggatccg atgcgcaagg tgcttcgagt
     2641 gggagcgatc gtgatcgcgt tcgccctgac ggcatggatc ggcgtggtgg cgctggctca
     2701 gggttcgagc acgcccaccc cgtcgccatc cccatcgccg accaccacca ccacgacgac
     2761 gacgacggcc acagcgacgg cttcgccttc gcccagtggg gtcgacgacg tgaagggcaa
     2821 ctgtgatgag gctgagcatg ccaacgatcc cgagtgccag ggcgtgacgc cggtcgcgga
     2881 ggacgacgac gtgaacgacg accaggatga agacgtcaac gacgatcacg gcgacgacgg
     2941 tggggatgac gacgacgaag gtgaggacga aggggacgac cacgagggtg ggaactccgg
     3001 ccccgggagc cccaactccg gccgcgggag cccgaattcc ggtcccggaa gcgccagttg
     3061 actcccggta acggtcccgg cgatggccgc gccctggcgc ggccgtcgcc gtccgatatc
     3121 tgttcgggcc cgtaggcttg aagcgtgagc aggatcctcg tgggcacggc gagctggacg
     3181 gacccgaccc tcgtgaaaga cgggcacttc tatccgccgg agg
//
LOCUS       DASUYD010000266        11786 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1543104, whole genome shotgun sequence.
ACCESSION   DASUYD010000266 DASUYD010000000
VERSION     DASUYD010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1543104"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(767..1267)
                     /locus_tag="VF195_13695"
     CDS             complement(767..1267)
                     /locus_tag="VF195_13695"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX6581903.1"
                     /translation="MSTMQPLRDEHEELLPHIEAIRTVADGVGSVSLQELRPQIADVS
                     EFLRDHLIPHAKAEEAVLYPEVERAMAAPRATAPMARDHVEVAALTAELGEIEARIDG
                     EDGSNTMLIDARRVLYGLYALVKLHFTEEEEIYVPILEDALDDQHARAMFDRMEAAAA
                     AARGST"
     gene            complement(1441..2334)
                     /locus_tag="VF195_13700"
     CDS             complement(1441..2334)
                     /locus_tag="VF195_13700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HEX6581904.1"
                     /translation="MVNVEVVADRRSVHDRSRRWFWRHFAEMVVAMLLSMAIFGAAVS
                     GIFALLGHGNLLHYGALRGFLMTIYMVVGMGLWMRHRRHGWASVLEMSAAMIVPYVLL
                     VGPFAAGLLAKGTFLGAMHVLMLPCMYLAMIHRREEYEQGHSGHGRQGHSAHRGRIFA
                     RFNRSVANPVTRLFAGWVPPFAVVRHRGRVSGRGYATPVWAFATRDGLVFALLYGTVS
                     DWAKNVLAADEVRVVRRGESRAYALPRLVRANEGQRLVPAILRPPLRLFGVHDFLRVS
                     VSLPAMLEPDKQNHSTHTAAL"
     gene            complement(2407..2541)
                     /locus_tag="VF195_13705"
     CDS             complement(2407..2541)
                     /locus_tag="VF195_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581905.1"
                     /translation="MSAVVVVWIGVAAVCCGLCIYGIGSVIRRSKEHREGRTPALTRR
                     "
     gene            3200..3481
                     /locus_tag="VF195_13710"
     CDS             3200..3481
                     /locus_tag="VF195_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581906.1"
                     /translation="MGFSFDSLRFTNAHVWQWPECIGVFAFGGSRHWEALVLAAIGGP
                     VPVAARIWVLAFAQRHRDRAGRTARALARAAYAAFILQGLPLIVAAWRP"
     gene            complement(3902..4684)
                     /gene="heR"
                     /locus_tag="VF195_13715"
     CDS             complement(3902..4684)
                     /gene="heR"
                     /locus_tag="VF195_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018386220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HEX6581907.1"
                     /translation="MPGADPTPVATGVTDARLIELRRWNLGLTAVHLVQAVLILMLAD
                     GFAIAVTSSFPEGPPGTPVPAPSTLFDVRIGWAVAIFLGLAALDHLLTATVSRGVYED
                     DLREGINRFRWIEYSFSATLMIILIGFYSGITSINGVIAVAGANVGMILFGWLQERMN
                     PPGRTSTTMLPFWFGALVGVAPWASIAVNIVGSKTVPGFVYGIVIAQLIFFFSFGVNQ
                     WLQYREVGRWTDYAFGEKTYLVLSLVAKSLLAWQIFAGSLAD"
     gene            4836..5557
                     /locus_tag="VF195_13720"
                     /pseudo
     CDS             4836..5557
                     /locus_tag="VF195_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968019.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
     gene            complement(5588..6085)
                     /locus_tag="VF195_13725"
     CDS             complement(5588..6085)
                     /locus_tag="VF195_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581908.1"
                     /translation="MQTQIEPEPTVRCFEPTGGFRRMTRIANVFMRPLLRSSAGKGIH
                     ELALLCFEGRRSGKHYEVPVEFHQLDGEPIILTASAWKRNLRGGADVELIHEGRRIAM
                     RAELIEDPDEVTRIYEALIERIGVANAKTTKIGLEVIGDRMPTHEQIRGAIAGRRTVV
                     RLRPR"
     gene            6317..6703
                     /locus_tag="VF195_13730"
     CDS             6317..6703
                     /locus_tag="VF195_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581909.1"
                     /translation="MLRSGRVLIVTDDLDTEVFWTAWVGLDGYDVETCRGPGAARDCP
                     RLHGIGCPLRERSDVAVVDLDCDEDALACSKLLDDGGTVFVRRSSVPPLGRRELSVAV
                     EDTRQHVQRLHGARIEHGTVRAVDLD"
     gene            6737..7096
                     /locus_tag="VF195_13735"
     CDS             6737..7096
                     /locus_tag="VF195_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581910.1"
                     /translation="MSTPRKALVVTPSLERAGLWSGWLRSAGYMSLSCAGPGLTHDCP
                     RLRGAICPLRQAVSLAVVDVNSDPSAQCCTILPDDGSTVSFRPVGSNRDQRSRLMGLV
                     AAPRVGSVGSAVEPHRR"
     gene            complement(7234..7527)
                     /locus_tag="VF195_13740"
     CDS             complement(7234..7527)
                     /locus_tag="VF195_13740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581911.1"
                     /translation="MERKTERKTERWKDFRPCPQCAFDFATGEGERACSWSECPYVPE
                     ELNVFCDNCRFDFATMEGNPPCSDPMACEHGVEARSHVENVREWRRALGFAVP"
     gene            complement(7749..8180)
                     /locus_tag="VF195_13745"
     CDS             complement(7749..8180)
                     /locus_tag="VF195_13745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003933978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HEX6581912.1"
                     /translation="MAGIALPDAARELLESGAVAHVVTIDEDGGPQVTAAWVGIEGEE
                     IVLATLPDQRKLRNLRRDPRIALSVPSTNTNDWGLLEYLVVYGRARVTEGGAAEILQR
                     LAHTYLGQDVVFPNMPDPPPGFVTRIMPERLGGVGPWNTRR"
     gene            8373..9170
                     /locus_tag="VF195_13750"
     CDS             8373..9170
                     /locus_tag="VF195_13750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK/PrmC family methyltransferase"
                     /protein_id="HEX6581913.1"
                     /translation="MSEERTRVTRELAKGGCVAPEAEADGLLTAAREGAGPIEELVRR
                     RLRGEPLAWITGSVTFCGRRVRIDPGVFVPRPHTEALARRAVELLPDQGIAVDLCTGS
                     GVVAVVLAAARPRALVAGTDVDPFAVACARRNGVPASEGDLDGPLPPALRGRIDLMTA
                     VVPYVPTEELHLLPRDVLEHEPRRALDGGSGGTRVLVRAAGVASRWLPPGGSVLLELG
                     LDQAAEMTAILTDLGFTDIRTHADADGQDRAIEGRRGPLPPGAVGQA"
     gene            complement(9141..9788)
                     /locus_tag="VF195_13755"
     CDS             complement(9141..9788)
                     /locus_tag="VF195_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011602984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX6581914.1"
                     /translation="MAFRGWKVEALEFFEGLEADNSKTYWQRHKDVYDTVVRAPMEEL
                     LAELEPEFGEGRIFRPYRDVRFSRDKSPYKTAIGATLGDGYIQVDARGLAVGSGKWEM
                     APDQLERYRESVAADRSGEDLTRIVSDARTKGLDVHGHEVLKTAPKGYPKDHPRIELL
                     RFKGLIAWQEWAAGAWLGTKRAKDRIVTFLERSKPLVDWLDTNVGDSSLPDRARR"
     gene            complement(9820..10791)
                     /locus_tag="VF195_13760"
     CDS             complement(9820..10791)
                     /locus_tag="VF195_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX6581915.1"
                     /translation="MRRTSRSLRLSAALIALAAATGLLAPGAGATEHDPVIVAVGDLA
                     CQSLTQGQGMGACRSGDVADLVREIDPDRFLALGDLQYNNGELSEFLRVWDVQFGDLR
                     EITMPAPGNHEYGTTDAAGYFAYFGAVANPPLGYYSFDLGDWHIVSLNSDICGDDPGC
                     GPGTPQGDWLRADLEANADAACTLAFMHHPRYDWRPFQKWIEDDGTTQFGGSETEPLV
                     PLWEVFADEGVDVVLVGHNHLYQRWAPQDAHGNSVSGGTIQFTVGTGGRQLYPFGPPP
                     VPENLEVTQNKAFGVLQMTLHEDSYDYGWVAVPGEPAFRDAGTVACT"
     gene            complement(10883..11191)
                     /locus_tag="VF195_13765"
     CDS             complement(10883..11191)
                     /locus_tag="VF195_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581916.1"
                     /translation="MDDCQLCVAEQVTVWLHEDDDCWVAECLVCRTPMIVWRSHGLPE
                     SELETELLTRLERIAGDRYGPDGYWVDPERRRIPDHWHAHARPAGGFFDPASDLYGTF
                     "
     gene            complement(11184..11648)
                     /locus_tag="VF195_13770"
     CDS             complement(11184..11648)
                     /locus_tag="VF195_13770"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEX6581917.1"
                     /translation="MRQITRASVSLTLLAACADDPAVPSDVHPVSTVTFGGTDSVLTV
                     DIADDPLERREGLMGIEELPTDQGMAFVWPGPVDSTFWMKDTLVPLSVAFVDESGRVI
                     DVVDMQPCASDPCPSYGIDEPFVLAIEANLGWFDEHGVGTGDRAELQSVIDG"
     gene            11703..11775
                     /locus_tag="VF195_13775"
     tRNA            11703..11775
                     /locus_tag="VF195_13775"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11736..11738,aa:Thr,seq:ggt)
BASE COUNT         1907 a         4063 c         3935 g         1881 t
ORIGIN      
        1 actcgtggcc gtggtgttct tcgtcctgcg aggctccgat gccgcccggt ggcgggaagg
       61 cggcgcgcct gaccagccag acgctcgaga gatcctcgcc gagcgcttcg cgcggggtga
      121 gatctccgaa ggggagttcg aacagcggac gcgcgtgctc gagcgccagc cgaagtaggc
      181 ggtcggggga ggctccggtg gctttcggcg cggtttccgg cccacgcgcg gtatcgctcc
      241 gcttccccga tcggaggctg cagcgcgggt tcgggaccga tacttcgccg acgatctgac
      301 gtacgtccgg gcagctcacg tcgtcgctgc gaccgtctgg gccctcttcg ggttttcccg
      361 ctcttcatac ggtggacgtc accactcgcg ccgacctcgc gcgcccgccg ttgaacgcga
      421 cgaccgacca gaaaccgtgt ggtcagggag ttctcagaac accccaccaa ccgatcaacc
      481 aacagcgggg tcttcctcgg aatcgagacc ctggcatctc agaatcccat cgacgaagtc
      541 gctcggtccc ttcgcggcac cgagccatga tggggacgtc agggtgtcgc atgcatctcg
      601 tgaccccgat cgacagtcag atccatgacc atgcagggac gccctccatc agggtcggtg
      661 ttccggcggc acagctccgc gatctacgtc gtccgacgac gcagactcgt aggcgcgttt
      721 cagccaactt ccatcgcgcc ggatcgacgt gcgcgcgatc acgccatcag gtggaaccgc
      781 gtgccgccgc cgccgcggcc tccatgcggt cgaacatcgc gcgagcatgc tgatcgtcca
      841 gcgcatcctc caggatggga acgtagatct cctcctcctc ggtgaaatgt agcttgacca
      901 gcgcgtacaa gccgtacagc acgcggcgcg cgtcgatcag catggtgttc gatccgtcct
      961 caccgtcgat cctggcttcg atctcaccga gctctgcggt gagggccgcg acttccacgt
     1021 ggtcccgggc catcggcgct gtcgcccgcg gcgccgccat cgcacgttct acctcgggat
     1081 acaggaccgc ctcctctgcc ttggcgtgcg ggatcaggtg gtccctcaag aactcggaca
     1141 cgtccgcgat ctgcgggcgc agctcttgga gggacacgga gccgacgccg tcggcgacgg
     1201 tcctgatcgc ctcgatgtgc ggaagcagtt cctcatgctc atcgcggagc ggttgcatcg
     1261 tcgacatcgg agcctcctcg ctcgctcatc tcagtctaga ggtgtgcgaa tcgctgagga
     1321 cgctcgaacc ggtggaggat cgataagcga cctagcatca gggttcggat gatcctcgac
     1381 tggaacggat cagccccggg acgagacggc cgcggccggc ggcccccggt ccctcgttgc
     1441 ctagagggcc gcggtgtgcg tggagtggtt ctgcttgtct ggctcgagca tggccgggag
     1501 agagaccgat acgcggagga agtcatggac gccgaagagg cggagaggcg gacgaaggat
     1561 cgctggaacc agtcgctgtc cctcgttggc tcgaaccaac cgggggagcg cgtaggctcg
     1621 gctctcaccg cgccgcacga ccctcacctc gtctgcggcg aggacgttct tcgcccagtc
     1681 cgagaccgtt ccgtacagca gagcgaacac cagaccgtct cgggtggcga acgcccacac
     1741 gggagtcgcg tacccgcggc cgctcacacg gccacggtgc ctcacgacag cgaacggcgg
     1801 cacccagccc gcgaagagcc gggtgaccgg gttggcaacg ctccgattga acctggcgaa
     1861 gatccttccc cgatgcgctg agtgaccttg gcggccgtgc cccgagtgac cctgctcgta
     1921 ctcctcacga cggtggatca tcgcgaggta catgcagggc agcatcagga cgtgcatcgc
     1981 acccaggaac gttcccttcg cgaggaggcc tgccgcgaac ggcccgacca gaagaacata
     2041 cggcacgatc atcgcagcgc tcatctcgag cacgctcgcc cagccatgcc ggcggtggcg
     2101 catccaaaga cccattccga cgaccatgta gatggtcatc aagaagccgc ggagggcacc
     2161 gtagtggagg aggttgccat gcccaagcag cgcgaagata cccgacaccg ccgctccgaa
     2221 gatggccatg gaaagcagca tcgcgacgac catctcggcg aagtgccgcc agaaccagcg
     2281 tctcgatcga tcgtggacag atcgtcgatc agccaccact tcaacgttca ccatcctcgc
     2341 ctcccctcgg agcctcggtg gagcaggttg ccttgccccg ctccaccggg gcggcctcgc
     2401 gtggcctcaa cgcctcgtca aggcgggtgt acgtccctcc cggtgctcct tcgagcgacg
     2461 gatcacgctg ccgatcccgt agatgcagag cccgcagcag accgccgcta cgccgatcca
     2521 aacaaccacc acggcagaca tgcccactca cctccctcgc cggtgtcggg aagccgactt
     2581 tcgggatcgc accggttgac cagacgttct ccccgaatgg gccatctggg tagagctgag
     2641 ggacccgcgc ggagcgggac ttggcgaccc cgcggcgcct ggcaccacgc cagccagcta
     2701 cgcgtgcggg aggttcttgc cttaacccac tcacacgggc gccgcgccca ggcgtgcggg
     2761 tgacgcgcga gatctctacc cggagagctc gacgaggtcc ctggacatca gggcgcgacc
     2821 atgggagctc gcccgagctg gaggaccgcc gtcccaccag cacgtaccag acctccagga
     2881 acaacaccga gaagatcgag atggagacgc cgatcgtcgg cacatacagg cccagtggcg
     2941 ctgacgacct acgggctcta ccgcgccgca gggttggatt ggagtctatg ggacgtgtag
     3001 gcggacgacc gaccctggac acggaccact gtggttcgtg gaggtcccgc tgatctctcc
     3061 tcggtgatct acgcggcctg gaccgcatgg cggtcgcgac ccagccgcgc ctccaacgcg
     3121 gtcatgacgg gaccgctccg tgctcgccac ctgatggcgc tgggcgccgg gatcgccatc
     3181 gccacccttc gtcctccgga tggggttctc gttcgactcg ctccgattca cgaacgcgca
     3241 cgtgtggcag tggcccgaat gtatcggggt tttcgcgttc gggggaagcc ggcactggga
     3301 ggcgctcgtg ctcgccgcga tcggaggtcc cgtgcccgtc gccgcccgga tctgggtcct
     3361 tgcgttcgct caacggcatc gggatcgcgc cggccggacc gctcgagcgt tggcccgcgc
     3421 cgcgtacgca gccttcatct tgcaggggct gccgctgatc gtggcggcgt ggcggccctg
     3481 acctttcgtg cgttcgacgt gccgctcgag gttaaggtcc tgctcgtagc gcccacggga
     3541 tcgcgggctc gtttcgccct cggctgaacc ctggtcacgc gcacgcgact cggccggatc
     3601 atgtgaggct tcgagcccat cgggaggagg gtggccgtgg gctcgttggc gggtcggcat
     3661 catcccggtc tggggcagca acgcagacca cactcctccg cccaccagcg aaccaaccaa
     3721 gcgaccaacc aacccaccca gccacgaacc gaccgactaa ccgacgaact cggccgagat
     3781 ccgggcgtgc tgacagcccc tgaagggccc gcgattcccg acctcggtgg gacgggcgcc
     3841 tcgagttccg cgactccgca gggcagttgc agaagccact cgccggcttt cggcccttcg
     3901 ctcagtcggc aagtgagccg gcgaagatct gccaggcgag cagcgacttc gccacgaggc
     3961 tgagcacgag gtaggtcttc tcgccgaagg cgtagtcggt ccaccggccg acctcccggt
     4021 attggagcca ctggttgact ccgaagctga agaagaagat gagctgtgcg atgacgatgc
     4081 cgtagacgaa cccgggcacg gtcttcgacc cgacgatgtt cacggcgatg gaggcccacg
     4141 gcgcaacgcc cacgagcgcg ccgaaccaga aggggagcat cgtggtcgac gtgcgccccg
     4201 gcgggttcat ccgctcctgg agccagccga acaggatcat ccccacgttg gcccctgcga
     4261 cggcgatgac tccgttgatc gaggtgatgc ctgagtagaa accgatcagg atgatcatca
     4321 gggtggcgct gaaggagtac tcgatccacc ggaagcggtt gatgccctcc ctcagatcgt
     4381 cctcatacac gccgcgggac accgtggccg tgagcagatg gtccagcgca gccagaccga
     4441 ggaagatcgc gaccgcccag ccgatacgca cgtcgaacag cgtgctaggc gcgggcaccg
     4501 gcgttccggg ggggccttca ggaaacgagg atgtgaccgc gatggcgaac ccatcggcca
     4561 gcatcaggat cagcaccgcc tgaaccagat gcacggcggt gagaccaagg ttccaccggc
     4621 gaagttcgat cagtcgagcg tcggtgactc cggtggcgac gggcgttgga tcggcgccgg
     4681 ggatgacgtg cctccctcga gagtggagtg gtcgggccgt ggtgcagaac cctgccccat
     4741 caggcaaaga caagctagca ggctctctct cggcaacgtt ggtgcctgcg cgctggccca
     4801 ccaaccagct tccgtgccgc gtgcgctgac gatgcgcccc caccttcatc tccaggttca
     4861 ccgaaccgac gcggccggcc gcggcctacc gcgaccgacg ggctctgctg gccggcgacg
     4921 ccgcacacgt gcatgccccg gaagggtctc aacaccggcg tgcaggatgc ggtgaacctg
     4981 tgggctggga agctggccca ggtggtcaag cggacatccc ccgagaacct cctcgatacc
     5041 taccacgccg agcgtcaccc agtagctgcc cgcgtgctgc gcaccacgat ggcgctcgtc
     5101 gcgcttcggc gcccggacga ccgcatccat caaggcggtg ggcgacgtcg tcaccgagct
     5161 cctgagcatg gacgagcctc gcgtacggtt tcgccgcgag gatgtccggc ctggacatcc
     5221 attacgacct cggcgaggga ccacccggct cgggccccgc atgcccgacc tcgacctctt
     5281 cacctcgggc ggcccgctgc gggtgttcac gatggcggcc ggtgctcctc gacctcggtg
     5341 agcccggcgg cttcgacatc acttcatggg cagatcgcgt tcagctgatc gacgccaagt
     5401 acttcgatac gtgggagctt ccgggactcg gcgcggtcac tgctcccacc gccgtcttga
     5461 tcccgcccga cggatacgtg gcctgggtgg gagacgcaac cagcgggggc tcgctgacgc
     5521 cctcacctcg tggttcggac caccaactgc ggcgtagcgg tcgggctcgt gcgctccggc
     5581 ctcgccctta ccggggccgg agccgaacca cggtgcgtcg gcccgcgatc gcgccgcgga
     5641 tctgctcgtg cgtcggcatc cgatcgccga tcacctcgag accgatcttc gtcgtcttcg
     5701 cgttcgcgac accgatccgt tcgatcaacg cctcgtagat ccgtgtgacc tcgtcgggat
     5761 cctcgatcag ttcggcgcgc atcgctatgc ggcgtccctc gtggatgagc tcgacatctg
     5821 ctccacctcg caggttgcgc ttccatgcgc tcgccgtgag gatgatcggc tccccgtcca
     5881 actggtggaa ctcgaccggc acctcgtagt gcttgccgct gcgccgaccc tcgaagcaca
     5941 gcagcgccag ctcgtggatc cccttgccag cagaggaccg caggagcggc cgcatgaaca
     6001 cgttggcgat cctcgtcatc ctgcggaacc cgccagtcgg ctcgaagcac cggacggtgg
     6061 gctcaggctc gatctgtgtc tgcatcggac gtcacctccc cgggttccgg tggacggcga
     6121 tcgccgtccg cctcccagga tcagtcaact ctcccgggga accgctctca tgggcacgag
     6181 gacccgtgat cggggggtct ctcggccacg agcgatgtcg acatctgtgg aggatggagg
     6241 acatagggga ctagggacct tcggccctct ccacgcggcg cccgcgtccg ggaggatgga
     6301 ccgggaggtg ggctggatgc tccgaagtgg tcgggttctg atcgtgaccg acgatctcga
     6361 caccgaagtc ttctggacgg cgtgggtcgg gctcgacggt tacgacgtcg agacgtgccg
     6421 cggaccgggt gcggcaaggg actgtccacg gctgcacggc atcggttgtc cgctgcgaga
     6481 gcggtcggac gtcgccgtcg tggatctgga ctgcgacgag gatgctctcg cctgcagcaa
     6541 gctcttggac gatgggggca ccgtgttcgt ccgtcggtcg agcgttccgc cgctcgggcg
     6601 ccgagagctg tcggttgcgg tcgaggacac gcgacagcac gtgcagaggc tccatggagc
     6661 gaggatcgag cacggaacgg tgcgggcggt ggacctggac tgagggtcgt gacggcgagg
     6721 gtcggagagg ggcgagatga gtaccccacg caaggcgctg gtcgtcacgc cgagcctcga
     6781 gcgggctggc ctctggagcg gatggctccg cagcgcgggc tacatgtcgc tgagctgcgc
     6841 cggccctggc ctcacccacg attgcccgcg cctccgtggt gcgatctgtc ccctccgcca
     6901 ggcggtgagc ctcgcggtgg tcgacgtgaa ctccgatccg agcgcccagt gctgcacgat
     6961 cctccccgac gacggctcga cggtctcgtt ccggccagtg gggtcgaaca gagaccaacg
     7021 atcaaggctg atggggctcg tggctgcccc tcgcgtgggc tcagtcggca gcgcggtcga
     7081 accgcatcgg cgctgaacaa acaggtggcg gggaggcggt ccgctggacg ccgatggggg
     7141 aaggagtcga tcgatcgttc cggggctgcg gcgagcatcg gtcgccggcg ttctgacgcc
     7201 gtcctcccgg cagtccggct ccctcgccgt cgcctaagga accgcgaacc ctagggcccg
     7261 ccgccactcg cgtacgttct cgacgtgaga gcgagcctcc actccatgct cacaggccat
     7321 cgggtcggaa caaggcgggt tcccctccat cgtagcgaag tcgaaccggc agttgtcgca
     7381 gaacacgttc agctcttccg ggacgtaggg gcactcgctc cacgagcacg cccgttcgcc
     7441 ctcgccggtc gcgaaatcga acgcgcactg ggggcagggc cggaagtcct tccatcgctc
     7501 cgtcttccgc tccgtcttcc gctccatctt ccgcctcctt cgctcacacg ctagttcggt
     7561 ggtcgggcag cggaacggct ctccggccct caacaagggg gccgaacgac ccggtcgatg
     7621 ggcgcggaag cgcggtcacg ggcatgcgat caggccgtcg cgacgagggc agagccggcg
     7681 tcgatccgga gggccctcgc gtggaaggcc ggcatccgcc gatcgcctct cagggagcgc
     7741 gctcgctgct agcggcgcgt gttccacggc ccgacgccgc ccaggcgttc gggcatgatc
     7801 ctcgtgacga accccggcgg cggatccggc atgttcggga acacgacgtc ctgccccagg
     7861 taggtgtgcg cgagacgctg gaggatctcg gcggcgccgc cctcggtcac ccgcgccctc
     7921 ccgtacacca cgaggtactc gagcaggccc cagtcgttcg tgttggtcga cgggaccgaa
     7981 agcgcgatac gaggatcccg gcggaggttc ctcagcttgc gttgatccgg cagcgtcgcc
     8041 aggacgatct cctccccttc gatcccgacc cacgctgccg tgacctgcgg tcctccgtcc
     8101 tcgtcgatcg tgaccacgtg cgcgacggcg ccgctctcga ggagctcccg agccgcgtcg
     8161 gggagggcga tccctgccac agttggatgc taccccctac acattgcaca gacgatcggc
     8221 tgaggagtct cctccgtgtc gcgggcagga ggagggtctc atgatcgaag gcccgaagga
     8281 tcgagaggac gatagcggtg tgccgaactc ctggaagctg cgctcgcgca cgatcgcccg
     8341 gctcccccga cctagggagg acgggagacc cgatgagcga ggaacggacg cgcgtcaccc
     8401 gcgagctcgc caagggtgga tgcgtggcac ccgaggccga ggccgacggc ttgctcaccg
     8461 cggcgcgaga gggcgccggt ccgatcgagg agctggttcg ccgccggctc cggggggagc
     8521 cgctcgcgtg gatcacggga tcggtgacgt tctgcggtcg tcgcgtccgg atcgaccccg
     8581 gcgtgttcgt gccgcgtccg cacacggagg cgctggcgcg gcgggcggtc gagctcctgc
     8641 cggaccaagg gatcgccgtc gacctctgca cggggagcgg ggtggtggcc gtggtgctcg
     8701 ccgccgcccg tccacgagcc ctggtggcgg gcacggacgt ggaccccttc gcggtcgcgt
     8761 gcgcgcgccg gaacggggtg ccggcatccg agggcgatct cgacgggccc ttgccccccg
     8821 cgctccgcgg gcggatcgac ctgatgaccg ccgtggttcc ctacgtgccg accgaggagc
     8881 tccacctcct gccgagagac gtgctcgaac acgaaccacg ccgcgcgctc gacgggggat
     8941 cgggcggaac ccgcgtgctc gtgcgcgcag ccggtgtggc gagccggtgg ctccccccgg
     9001 gggggagcgt gctcctggag ctcggcctcg accaagctgc agagatgacc gcgatcctca
     9061 ccgatctcgg cttcacggac atccgcacgc acgcggacgc ggacggtcag gatcgagcga
     9121 tcgagggccg acgggggccg ctaccgccgg gcgcggtcgg gcaggcttga gtcgccgacg
     9181 ttcgtgtcga gccaatcgac gagcggcttc gaccgctcga ggaacgtcac gatccgatcc
     9241 ttcgctcgct tcgttccgag ccatgccccc gccgcccatt cctgccacgc gatcaatccc
     9301 ttgaacctga gaagctcgat ccgtgggtgg tccttgggat agcctttcgg cgccgtcttc
     9361 aacacctcgt gcccgtggac gtccagtccc ttggttcgcg cgtccgacac gatcctcgtc
     9421 agatcctcac cggaccggtc ggccgcgacg ctctcgcgat agcgctcgag ctggtcgggc
     9481 gccatctccc acttcccgct gccgacggcg agaccgcgcg cgtccacctg gatgtacccg
     9541 tcgccgagcg tggccccgat cgcggtcttg tacggcgact tgtcgcggct gaagcgaacg
     9601 tcgcggtaag ggcggaagat ccggccttca ccgaactcgg gctctagctc ggcgagcagc
     9661 tcctccatcg gtgcgcgcac caccgtgtcg tacacgtcct tgtgtcgctg ccagtacgtc
     9721 ttcgagttgt cagcctccag gccttcgaag aactcgagcg cctccacctt ccatccgcgg
     9781 aacgccatcg gacccgacct cctcgaccca cgtcccggat caggtgcagg ctaccgtccc
     9841 cgcgtcccgg aaggccggct cgccgggcac ggcgacccat ccgtagtcgt aggagtcctc
     9901 gtgcagcgtc atctgcagca caccgaatgc cttgttctgg gtgacctcga ggttctcggg
     9961 taccggaggg ggaccgaagg ggtagagctg ccgcccgccc gtcccaaccg tgaactggat
    10021 cgtgccgcca gagacggagt tgccgtgggc atcctgcgga gcccaccgtt ggtagagatg
    10081 gttgtgcccg acgagcacga catcgacgcc ttcgtccgcg aacacctccc agagcgggac
    10141 gagcggctcg gtctccgagc cgccgaactg cgtggtgccg tcgtcctcga tccacttctg
    10201 gaacggccgc cagtcgtagc gagggtgatg catgaacgcg agcgtgcagg cggcgtccgc
    10261 gttcgcctcg aggtccgccc ggagccagtc accttggggc gtgcccgggc cgcagccggg
    10321 gtcgtccccg cagatgtcgg agttgagcga cacgatgtgc caatcgccga ggtcgaaaga
    10381 gtagtacccc agcggtgggt tggccaccgc gccgaagtag gcgaagtaac cggcggcgtc
    10441 ggtcgtcccg tactcatggt tcccaggtgc cggcatcgtg atctcccgga ggtcgccgaa
    10501 ctgcacgtcc cacacccgga ggaactccga gagctccccg ttgttgtact gcagatctcc
    10561 gagcgcgagg aaccggtcgg ggtcgatctc gcggaccagg tccgcgacgt cccccgatcg
    10621 gcaggcaccc atcccttgtc cctgcgtcag cgattggcac gcgaggtcgc cgacggcgac
    10681 gatcacggga tcgtgctccg tcgcaccggc gccgggcgcg aggaggccgg tcgccgcagc
    10741 cagcgcgatc agggcagccg agaggcggag cgaacgggaa gtgcggcgca tggggaactc
    10801 cttccgggcg gggcgcgtat tcaagcacgc cggggacggc catgggaacg actcccggac
    10861 accgcgactt ccgacctcgg cgtcagaacg tgccgtacag gtcgctcgcg gggtcgaaga
    10921 agccgccggc cgggcgcgcg tgcgcgtgcc agtgatccgg gatccggcgt cgctcggggt
    10981 cgacccagta gccgtccggc ccgtaccggt cgccggcgat ccgctcgagc cgggtcaaca
    11041 gctccgtctc gagctcggac tccggcagcc cgtgggagcg ccacacgatc atcggggtcc
    11101 ggcatacgag gcactccgcg acccaacagt cgtcgtcctc gtggagccac acggtgacct
    11161 gttccgcgac gcagagctgg cagtcatcca tcgatgacgc tctggagctc cgctcgatcg
    11221 ccggtcccga caccgtgttc gtcgaaccag ccaaggttcg cttcgatcgc gaggacgaag
    11281 ggttcgtcga tcccgtagga ggggcacggg tccgacgcgc acggctgcat gtccacgacg
    11341 tcgatcacgc gcccggactc atcgacgaag gcgaccgaga gcggcacgag cgtgtccttc
    11401 atccagaacg tgctgtcgac gggccccggc cacacgaacg ccatcccctg atccgtgggg
    11461 agctcctcga tgcccatcaa tccctcccgg cgttcaagcg gatcgtccgc gatgtcgacc
    11521 gtgaggaccg agtccgttcc accgaacgtc acggtcgaca ccgggtggac atccgagggc
    11581 accgctgggt cgtcagcgca cgcggccagg agggtcagcg aaaccgaggc cctggtgatc
    11641 tgccgcatca cgtcatcgtg ccatggtcag cgaccgaccc attgttatca tcgagcactg
    11701 ccgccggagt agctcagtcg gcagagcgcc tccatggtaa ggaggaggtc aggggttcga
    11761 ttcccctctc cggctccgga agaagc
//
LOCUS       DASUYD010000267        28205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Actinomycetota bacterium isolate SMAG_U1886
            k141_1101232, whole genome shotgun sequence.
ACCESSION   DASUYD010000267 DASUYD010000000
VERSION     DASUYD010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37961217
            Sequence Read Archive: SRR25158380
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Actinomycetota bacterium (soil metagenome)
  ORGANISM  Actinomycetota bacterium
            Bacteria; Actinomycetota.
REFERENCE   1  (bases 1 to 28205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 18:54:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,787
            CDSs (total)                      :: 2,736
            Genes (coding)                    :: 2,699
            CDSs (with protein)               :: 2,699
            Genes (RNA)                       :: 51
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 47
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 37
            CDSs (without protein)            :: 37
            Pseudo Genes (ambiguous residues) :: 0 of 37
            Pseudo Genes (frameshifted)       :: 15 of 37
            Pseudo Genes (incomplete)         :: 26 of 37
            Pseudo Genes (internal stop)      :: 3 of 37
            Pseudo Genes (multiple problems)  :: 6 of 37
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28205
                     /organism="Actinomycetota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101232"
                     /isolate="SMAG_U1886"
                     /isolation_source="soil"
                     /db_xref="taxon:2900548"
                     /environmental_sample
                     /country="China"
                     /lat_lon="26.86611111 N 100.1480556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..903)
                     /locus_tag="VF195_13780"
     CDS             complement(<1..903)
                     /locus_tag="VF195_13780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581918.1"
                     /translation="MHEARPRDDGKSLLRAMMRKPEGQVVDLDALLKEVGARPLPSEL
                     WAEHSTVASMAAEVRATRRSIAEPARRRTVHRPVTRAAVAAATIVITAGSSLAMTGHL
                     PDGARDLASEAFAMFVPARERLSAASAPSPGTEQSFATPGQGVTGEDTLADDHEEPSD
                     DGVTGVGGGIGRGNGGTVGGAPERGVADENTDGENGSGEGTAGNGSEDGSSEDGNGNV
                     GHDAGGGAGVDDVGNGGGGTDIGGIDGGGNAGGGGNAGGGGNAGGGGNAGGGGNAGGG
                     NGGGGGNGGGGIEDSGGGDEGAGEP"
     gene            complement(903..3188)
                     /locus_tag="VF195_13785"
     CDS             complement(903..3188)
                     /locus_tag="VF195_13785"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX6581919.1"
                     /translation="MTGSPGGPLPIEFRTLRVGMRVTLLAPLAAAAMFVLATRARLDL
                     GGYGLVLTGLAVAAALATLLPWDRLLATRGGMRLLAVWAVTALGMVTVGVWVTGGTHS
                     PLVLLYALTTVFFAVAFSPRGQVALLAMTLVAYGIVLRAADQGPLPLIVLSVLTFLAG
                     FLVQEIRVQVRAAWDARAEADRRWALLATVSAAARDMSSSTVDADQVLETTIEAVGGL
                     GFPFARVFVREGDDWHTVPGVPAPESSVPEVVRTTAETGVFAVFRGRTIGLPLFGAAV
                     PIAVSDGVAAVLVVGTEAAPGPSTQDVEVFRMLSSQASLALENVRRFDEQRQVIQRLA
                     DLDRMKNDFLSTVSHELRTPLTVITGMGKTLEHQWATIDDEIRADFLTRLNANAATLD
                     HVIAQLLDFSKLEAGQLRPDAQDVDLSRLVPAVVDRLSTLLSRHRVELDVPSALCAQA
                     DPGLIERIIENLLSNASKYTPAGSRVVVSARDEGADVVVAVSDDGPGIPESEVARIGE
                     RFFRGGDVHTRSTRGTGLGLAVVSEILQLHGSRLDVESRVGEGSRFSFRLAKDPQIVT
                     QPTQRRREPVDAVPPGARSLETPTLGLRGERFETVMTSATTGVEWAIGLLYREFHPAV
                     LRYLRARGASAPEGAAEQVWIQVAQGLQRFSGDEIAFRRWLFDLARRTLEAGDRVVGP
                     IELEERTREPMSEALSRVAALPPDHADVLLLRGLGHLDVDDVAALTGQDTGAVRLLEM
                     DALRLLRDADDADEAETAEVG"
     gene            complement(3429..3686)
                     /locus_tag="VF195_13790"
     CDS             complement(3429..3686)
                     /locus_tag="VF195_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581920.1"
                     /translation="MDISLLREEIEKLVLLEEVAERRQKMIEEQLDLATKAGLSAEEI
                     RNELGLSKESIRRLLDQKTPALAERLGISQESAENLSESIG"
     gene            complement(3705..3917)
                     /locus_tag="VF195_13795"
     CDS             complement(3705..3917)
                     /locus_tag="VF195_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007293143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HEX6581921.1"
                     /translation="MTDSVYRVTEVIGTSSTSWEEAAKNAVETAATTLRDLRVGEVVK
                     FDVTIEDGKVTSYRARVNISFKYESS"
     gene            4054..4449
                     /locus_tag="VF195_13800"
     CDS             4054..4449
                     /locus_tag="VF195_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581922.1"
                     /translation="MGPLLDESGGDAAPRTARQLPDGGYDFESRIEGLKRRRRSAPDP
                     FERHQGPDMSMGEFLNTRSEVEAYVEPETVVSPRSVVLVDGAGEWRRFRPSEDSVLRR
                     VAGERGMLIFDASRTGYPSRMRRGPGPAT"
     gene            complement(4446..5006)
                     /locus_tag="VF195_13805"
     CDS             complement(4446..5006)
                     /locus_tag="VF195_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581923.1"
                     /translation="MMPSELTLRYLRALADLPHDRPAALAALETCFRNGETPEPLDGL
                     MPGRVITTTVGYGLDPIIVGLARVWMPWKGKTFDQEAKEGRNAFSTSFRVVQRTLWPG
                     YDLDWPLGDRRYATFPFTTWDGPSVHTPGGADVFKIDYEHPQSPWIVRDILDELVRID
                     DELLLGQVLLRIRGRLHRVAWFELRR"
     gene            5056..6699
                     /locus_tag="VF195_13810"
     CDS             5056..6699
                     /locus_tag="VF195_13810"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581924.1"
                     /translation="MRVRRALGLIGVAALTVACTQGGSAQPSGTRAPSRSSAVTPDPG
                     PIRAERLLVLTDDGNVVVVRADGGDPRRLTEFDSDGATVEVRHPVWSPDGRSVAWAEL
                     EIGDQGARPRIVSIGSDGSRRTEFPVDTGAFFLQWDPTSSRIAYLGNFQGSVGMGVAG
                     RAPIGDPVATTLGVGRPFYLSWAPGGLELLVHVGDETLGRLDLEGELRDVGDAPGIFQ
                     APVWLADGRMFYATAERDRQTLVVRDGDRLRELVEFEGAIEFVVDPRGERIAYRVDDG
                     AGPGGVEVIETTTARSQVVSASPTFAFQWSPDGERLLLLTPDEDGETGAHRWLVWDGD
                     VTRPVGPAFVPSPSFLRDYVPFYGQFAQAMTPWSPDGGAFAFAGLIGDQAGVWVQDFR
                     AADPTFVLEGGSVVAWSPTPTCRPTVDCASVLSETTVFDAAGGMPFFEQLVARFYEGV
                     AGDPVLRPLYPDDLAPSTRHLTLFLAQYWGGPTTYDEERGHPRLRMRHAPFAVGPAER
                     DRWLVHMRAAVTTMAPPEPIERALVDYFEMAAEAMRNRD"
     gene            complement(6704..8395)
                     /locus_tag="VF195_13815"
     CDS             complement(6704..8395)
                     /locus_tag="VF195_13815"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX6581925.1"
                     /translation="MPERYSRAEHARRHGPTAGDRIRLGDTDLWIRIERDLTDPADQA
                     LWGYAKNWRSGMVQHDRATTESELDTIIASAIVLDPLLGVLKADIGIKDGRIAGIGRA
                     GNPDITDGVDLTIGPNTWPVPCHGLIATPGAVDSHVHLLSPRLVPVALSAGVTTLITA
                     GFEEPPWRMLRTLEAFELLPVNIGLQPSARTGVPGQLEVAIEAGAVGLKIHEDWGAYP
                     EVIDEVLRVADAHDVQVCLHTDGLNESCELADTVGAIAGRAIHAYHVEGSGGGHIPDL
                     IGIVREPNVFCSSTTPTIPYGAATAAEQQDMKLIVHEGNPALPDDVVAARELVHPATM
                     EAEGPLHDLGAISIVNSDSQGMGRIGETLRRTFQLAHAMKVAAATTEAGGSGRDEPDD
                     NDRVLRYLSKVTEEPARVHGISDEVGSLSSGRLADLVLWEASHFGVKPVMVFKGGVVA
                     WTAIGEGNASVHGAEPTRYGPDWGGTGLAPARVSTTFVSAAAIDAGIARTLGTRRRLV
                     AVRGTRAIRRDGLARNRTVPSIEVSAADGTVTLDGRVLRIDPVTEVRLSRRYLLA"
     gene            complement(8388..8981)
                     /locus_tag="VF195_13820"
     CDS             complement(8388..8981)
                     /locus_tag="VF195_13820"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013132846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX6581926.1"
                     /translation="MQLTPTEEERLRVFTVAQLARSTLAKGLRLNAPEAVALVCDEMH
                     AAGRGGSSFDEVVAAGRDAVTPEQVMEGIAAIVQEIRVEILLEDGTRLFVIREPFGPA
                     GSDGPGAIRFADGDVELGPGRDRIHLTVTNRGQHPIRVSSHFPFWRSNEHLTFDRQAA
                     DGFRLDLPAGDSLRWAPGETHEVDLVRYGGTGAPAGA"
     gene            9042..10418
                     /gene="chrA"
                     /locus_tag="VF195_13825"
     CDS             9042..10418
                     /gene="chrA"
                     /locus_tag="VF195_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HEX6581927.1"
                     /translation="MTRPPDERSPDPAPRTPPTFRDASRFWIWLGFVNFGGPAGQIAL
                     MHQELVERRRWIDEGRFLHALSFCMLLPGPEATQLATYVGWLLHRVRGGLIAGVAFVL
                     PSFFLLLGLSWLFVTHGDLRWVAGAFAGLAAAVVAIVAHAMVRLARTTLANGLMAAVA
                     AISFIAIFVLDVPFPVVIGAAVLLGLVGGRIRPALFAVGAGKELEERAAASVVGTTDT
                     PRPTWRRSAVVLVVGLAAWWLPIVAMAVWRGDAGTLTDMGWFFSKAALVTFGGAYAVL
                     AYVDQAAVGVFGWLRPGQMAVGLGLAESTPGPLIMVVEFVGFVGAYQHPGGLPPFVAG
                     VLGATVAVWATFAPCFLWVFLGAPSIERIRGDARLASALQTVMAAVVGVIANLAVTFA
                     VVTLFRSVRTVAFLGGELPVPVWSEVDLFAVAVAGIAFVGLWRLRWRVVPMIVASAAA
                     GLVVKGLL"
     gene            complement(10402..11112)
                     /locus_tag="VF195_13830"
     CDS             complement(10402..11112)
                     /locus_tag="VF195_13830"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEX6581928.1"
                     /translation="MDPSRRRRVVIVAIATIVAAVCIALGFWQLQRLADRRALNAEIR
                     AARSAPPTTVRTADDFGALSAFRSVVVEGTYDIQGEVLLYGRSLGGEPGHFVVTPLAL
                     DDGSGVLVIRGWVPFSLDRAPVVEAAPAVRDVVVDGWLIAPESRGRGRPDRDGIVRTL
                     DISGIGDRLPFDLAPFAVQLRAQDPPNTELPVPVPLPELSEGPHLSYAIQWFCFAAVA
                     VAGAAILVRRERSPTAAL"
     gene            complement(11125..11904)
                     /locus_tag="VF195_13835"
     CDS             complement(11125..11904)
                     /locus_tag="VF195_13835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017521496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX6581929.1"
                     /translation="MRGEANGRVALVTGAGSPTGIGFATARILAREGAALAIASTTDR
                     IHDRAAELRGTGAEVNGYVADLTDRAQVRRMVDDVTDRSGRIDILVNNAGMVSVSMDA
                     TSDGAFLDIDDAAWDRDLAMNLDTAYNVTKRVLPGMVERGWGRIVMVSSVTGPIVTYP
                     GLTAYSAAKAGMDGLMRGVAIEVASRGVTVNSVQPGFVATGSQTPEEHEIGHHTPIGR
                     SGTAEEIGEVIAFLASDRAGYVTGQTVVVDGGNVIQEAKGP"
     gene            11966..12673
                     /locus_tag="VF195_13840"
     CDS             11966..12673
                     /locus_tag="VF195_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581930.1"
                     /translation="MDPARPLLEIQEHDTAIDRLQARIAALEAGADVAAALAEANEAE
                     RVMGELRLKLDELSRDQQRFEHEIDSMSQKAAAEERRMYDGSVANARELDSIRHEVDN
                     LKKRRTDREDELLGLMEVREELEAQEREADTRSTALRARVDEVGGIAAEELGTLTDEV
                     KQRTEKRAALAEAVDPEYLELYDDLRPQKKGVAAVELVDGVCLGCHEQLSSVYRDRLK
                     RVEGPKRCEHCRRILVI"
     gene            12640..13077
                     /locus_tag="VF195_13845"
     CDS             12640..13077
                     /locus_tag="VF195_13845"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEX6581931.1"
                     /translation="MRALSADPGHLIVSCDGASRGNPGPAGIGAQVTDESGAILGEIA
                     EGIGETTNNVAEYTAVIEGLSLAQELGAKTVTLRSDSLLLINQLTGRYRVKSEHLQPL
                     HRRARSLGAGFDRITFEHVPRERNSAADTLANLGVDRWLAGER"
     gene            13096..13499
                     /gene="rnpB"
                     /locus_tag="VF195_13850"
     ncRNA           13096..13499
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF195_13850"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            13755..14150
                     /locus_tag="VF195_13855"
     CDS             13755..14150
                     /locus_tag="VF195_13855"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX6581932.1"
                     /translation="MDIEKLRRILLFEDLHDRALERIAELSTEFEEPAGWVLVEVGQP
                     GSGMFVLEEGTVEVQTPDGRAWELGPGEFFGELALLTDQPRNARIRAKSPVRCLAISR
                     MDFQKLLSEEPEIAVAMLPKLAQRVDAPH"
     gene            14205..15182
                     /locus_tag="VF195_13860"
     CDS             14205..15182
                     /locus_tag="VF195_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018090475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rv2578c family radical SAM protein"
                     /protein_id="HEX6581933.1"
                     /translation="MDQLFEIARKTFDTPEFRGMTFIESEAKSIINRVPGGALPFDWT
                     INPYRGCSHACVYCFARPTHAYLDMNAGRDFETKIVVKVNAPDVLRRELAAKRWKGEH
                     IAMGTNTDPYQRAEGKYRLMRGILEALVDYRNPFSILTKGTLILRDVDLLQRAADVAT
                     VSAAFSIGTLDETVWRETEPGTPHPKARIDAIRTLTEAGIPTGVLMAPILPGISDRPE
                     QLRELVEAAIDAGATHVSPILLHLRPGVREEFLPWLEDAHPDLVPRYLEMYRQPYGPK
                     ALRESLGRRVSSMVSAAGGIRRSVEPARRFFRRDRERPRDQRAEQLRLL"
     gene            15582..15890
                     /locus_tag="VF195_13865"
     CDS             15582..15890
                     /locus_tag="VF195_13865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581934.1"
                     /translation="MAHWPRTVTGKSFYVALIAVAVPLTVWFLVTVPSYVILALGGIA
                     IISAALYLWRRGLDAAREKAWVGEFSFGDVVGRMKARDALDRAREPGTLTAAAVSSGG
                     "
     gene            complement(15876..16853)
                     /gene="lipA"
                     /locus_tag="VF195_13870"
     CDS             complement(15876..16853)
                     /gene="lipA"
                     /locus_tag="VF195_13870"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX6581935.1"
                     /translation="MASMAHGADHNPNKRRLHLPIVGVTNGPGVGSVTGRKPPWLKVR
                     LTTGPNYEDLKGIMRERSLHTVCEEAMCPNIRECWEEREATFLILGSKCTRRCGFCDV
                     MTAKPDPVDEGEPARIAEAVGEMGLRYVVLTGVARDDLEDGGARIWASAIRAAREAVS
                     GIGIEVLPSDFKGGERDIATVLDAEPDVFAHNLETVPRLHDRIRPAFGYDRSLEVLRF
                     AKRHRAVQVTKSNLILGMGERPEEIPTAMQDLRDARIDILTMGQYLQPTTFHLPVDRW
                     VHPDEFSEWKRIGEEMGIAHVEAGPLVRSSYHAGKQLRRAVESGVSAPA"
     gene            complement(16879..17868)
                     /locus_tag="VF195_13875"
     CDS             complement(16879..17868)
                     /locus_tag="VF195_13875"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006677953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6581936.1"
                     /translation="MTEKNVVTAVRDALHDEMANDERIVLLGEDVGARGGVFRISAGW
                     MEEFGEQRVIDTPLAESGIVGIAIGMALHGLLPVAEIQFADFIHPAFDQVVSEAARLR
                     YRSNGDFGVPMVIRTPYGGGIHGALYHSQSIEAFYAHIPGIKVVVPSTPADAAGLLRS
                     AIKDPDPVLFLEHKKTYRAIKGEVDDGAYLTPIGVAEVKRGGDDLTCVSYGYSLHLCL
                     DAAQRLEEGEGFSVEVVDARTLAPLDIDTIVGSVKKTGKAMVVYEDNRTYGAGAEIAS
                     TIAEQALFDLDAPILRIGGPDIPAMPFAAPLEHYFMEPEPDHIYARMRELARS"
     gene            complement(17865..18884)
                     /locus_tag="VF195_13880"
     CDS             complement(17865..18884)
                     /locus_tag="VF195_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007503767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6581937.1"
                     /translation="MSTRVAETPHGALGLSADDLASMHRLLLTSRLLDEAALRQNRMG
                     RAPFVVPAAGHEGCQIGTAWAMRRGTDIWLPYYRDLGIVLSAGMTPYEVFLGVFAKAD
                     DPSSGGRQMPAHWGSRRLGIISGSSPIATQVPHAAGIAYAAKYRGEDAVVGCWFGDGA
                     TSEGDWHEGLNFSGIHRLPVIFVCENNQYAISVPQSKQMAVKDVASRAEGYGFPGVVV
                     DGNDVLACYGAMKTAHERARAGEGPTLIECKTYRFMPHTSDDDDKSYRSREEVELAKA
                     RDPLIQFAGYLREHDLLDDAAQEALRAEVKQEIDDAIRAAWDAPDPDPDTALHHVFRE
                     QEDER"
     gene            19078..19371
                     /locus_tag="VF195_13885"
     CDS             19078..19371
                     /locus_tag="VF195_13885"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEX6581938.1"
                     /translation="MRGLQRMVDEDVYLIDILTQVNSVTAALKAVGMGLLDAHVRHRV
                     TESIEAGDGEQKVEELMSAVAILRPLLLGGAARNRTGGRARCHPRGRRRDWPE"
     gene            19343..20206
                     /gene="trxA"
                     /locus_tag="VF195_13890"
     CDS             19343..20206
                     /gene="trxA"
                     /locus_tag="VF195_13890"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX6581939.1"
                     /translation="MAVVETGRSDARFATEDTRSGNSEMTASTNVIEVTDQTFEQVVV
                     EGSKERPVVVDMWAAWCGPCRQLTPTLEKVADERSGAFLLAKLDTDANPVTASAFGVQ
                     SIPTVIAFKDGQAVTGFIGAYPEPEVNRFIDSILPTEAEKHAEEARTVEASGDLATAE
                     TGYRQVLEEDEGNRDAAIGLARILVERGDLDEAEQFLGKHRPDPEAEKLLASIAVRRW
                     ANSPADGVLGEAQRVVASGDLSGGLAGLLQALKEDREAARDAMITVFTAVGDEDPVVQ
                     EYRRLLSAALF"
     gene            20211..20951
                     /locus_tag="VF195_13895"
     CDS             20211..20951
                     /locus_tag="VF195_13895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX6581940.1"
                     /translation="MDVRDQIREWWDDDADAYDVAPGHSLSDPVEAAAWRAVLLSLLP
                     PAPALVLDVGAGTGSMSLLAAEMGHVVTALDLSQGMLERAERKASERGLRIDFVVGPA
                     EEPPLGSFDVVTARHLLWTLPAPAEALAAWRRAVRDDGRLVLFEGSWAGEGPWSRAAG
                     AVASSAERLMGVREDHHAPYPPGVIDRLPLQGASSPAPFVEAVRSAGWSRIRLVRLRD
                     VEWSIERRAPWPLGWLRRRPRYAIVAES"
     gene            complement(20927..21172)
                     /locus_tag="VF195_13900"
     CDS             complement(20927..21172)
                     /locus_tag="VF195_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581941.1"
                     /translation="MTGRVTLRGVTGADLPIFFAHQRDEERPHSAHRRRAQRRIDPRP
                     REVLVRSSPSIGVEIAGEPVTEMLFRREGPYDSATIA"
     gene            21272..21574
                     /locus_tag="VF195_13905"
     CDS             21272..21574
                     /locus_tag="VF195_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX6581942.1"
                     /translation="MDETWVTLGVMCMGLGGVVAGLVGVVLPEGRRLGALFALIAGAG
                     VGLVVVGLGALLDERNEPTELTFFLASLLGLLTVCGASWAVWRRAAGSGPSTGEPA"
     gene            complement(21571..22269)
                     /gene="lipB"
                     /locus_tag="VF195_13910"
     CDS             complement(21571..22269)
                     /gene="lipB"
                     /locus_tag="VF195_13910"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010925.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX6581943.1"
                     /translation="MRREALLVRPEGPVRYSLANDAMHRLAERRLAGEIPDVVILLEH
                     PPVFTGGRRARPDELLWSPGELAARGAEVYRIDRGGAFTFHGPGQLVGYPILDLGPKP
                     DAAAHLRRMEEAVIRAGEDLGVSLQRREDVQTGVWHGDDKVCAIGVRLLRARVTVHGF
                     AVNCDPDLSWFDGIVACGLPGHGVTSLSRLAERPVTVAEVAPLLERHLADVFDIELVL
                     APDGIAAALRPVAV"
     gene            complement(22280..23752)
                     /locus_tag="VF195_13915"
     CDS             complement(22280..23752)
                     /locus_tag="VF195_13915"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX6581944.1"
                     /translation="MTTIKMPQLGETIVEGTILKWLKQEGETIERDEPLFEISTDKVD
                     TEVPSSVGGTVTKILVQEGQTVPVGTDLAEVSEDGSGGGAASGGQASSDAGAPSQNES
                     QRDPAPETAPETVPETVKEAPSESAEAVAEGSAAEMPAVEPATVQGAEATAASGTAPG
                     QAADPAPPDGGSGDLPDRGPRSRILSPLVRRLADEHGLDLSRVEGSGTGGRITKKDVL
                     AAIDGDGAAAAPATAEVPAAAPAPAPAAATSPAPQAAAGPDEEVAPVSHIRKLIGQHM
                     LASLQTSARAWTMVEVNVDHLVKLRERAKESFKVKHGVNLTYLPFVIRATCDALMTHP
                     EVNSSLRGDEIVRHRAVHMGIAVSYDEGLIVPVIRAADSMNTVGLARAIADLAGRARA
                     HQLKPDEIQGSTFTITNPGPYGSIASVPIINQPNTGILSFDAIQKRAVVIDDTIAIRS
                     MVNISMSWDHRTIDGEIATRFLARVKQNLESWDFAEDVAV"
     gene            complement(23769..24839)
                     /locus_tag="VF195_13920"
     CDS             complement(23769..24839)
                     /locus_tag="VF195_13920"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX6581945.1"
                     /translation="MAQAQDTRPKNREVETGAAGHGAEPRGKRTEDGQATYLVAIAEA
                     LWEEMERDERVYMLGEDIGVYGGAFKVTEGFYDRFGADRVMDTPIAEETIVGMAIGSA
                     MEGFRPVAEFQYADFMTSGFDEISTALARVHYRSGVPLPVVLRGPSGGMVRASSFHSI
                     NPEPWFAYSGGIKIVCPAFPADAKGLLKSAIRDDNPVMYLEHKWIYRRVREQVSEDPD
                     FLVPIGSANVKREGDDVSIITYGAMVHRALEAAQTLEAEGVSAEVVDLRTIVPMDEET
                     VLRSIEKTARAVVLSESYRFVGIAAEVASTIAEKAFEHLDAPVVRLAPPNVPIPFSPT
                     LEDAFLPGVDDIVATVKKLSAW"
     gene            complement(24873..25931)
                     /locus_tag="VF195_13925"
     CDS             complement(24873..25931)
                     /locus_tag="VF195_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX6581946.1"
                     /translation="MATTQTRQRKDTDPRLSDDDLKRALYLLKLCRYFDERMESLYRQ
                     GRLPGAIYSGRGQEGTHIGVVLPLGPEDSLFPTHRDLSAQIAKGLDLKRIMAQYWGRI
                     DGYTRGRDGNSHIGDWYGSKTWTVMSHLPIAYPVACGAALAYKRRGEPHVAMAICGDG
                     STSNGRWHESLNMSAIAQLPVVWVVNNNLYAYSAPNELEFSVPTIAERAAAYGMPGVR
                     VDGADVLAVEAAAREAVERARGGGGPSLIESVSLRWKGHAGHDPARYVPKELLEEYMR
                     DKDPVKILEQRLLSDGVVDDQEVADIQGRIEKEFEEGYEFAQSSPFPEPEDFSKGLWV
                     EDGYWTSEPGRGGGTEAS"
     gene            complement(25940..27322)
                     /gene="lpdA"
                     /locus_tag="VF195_13930"
     CDS             complement(25940..27322)
                     /gene="lpdA"
                     /locus_tag="VF195_13930"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX6581947.1"
                     /translation="MADAYDVLVVGAGTGGYSTALRAAQLGKRVALVERHDRLGGTCL
                     LRGCIPTKALLQSAAVMDTVNRAQEWGIKASGDPDWSAVRAFEDKIVDKLVKGVTGLI
                     KTRGITVLKGTAKLLPGPALEVEGQRVDATDVVIATGSAPRLLPGVEVTDRVITSDEA
                     LRYDRIPGSAVVIGAGAVGLEFASLYRSLGAEVTLLEALPGLAPLEDEDVSAEVAKAF
                     RRRGIKASSGASVKEIADTGESVDVTFEAGGKAERVTADICLVAIGRGPVTEGLGLED
                     AGVAVHEKGFVDVDGQLRTNVEHVWAVGDVANTPLQLAHVAFTEGYAVAERIAGETVP
                     DIDYVNIPRVTYCTPEIASVGLTAGQAKDAGRKVATAHLDFRGIGKANMLGEGGFVKV
                     VSEQGGGPVLGVHMIGPHVTDLIAEAMLITNWEAVPAEVAALIHPHPSLSEAIGEAHL
                     ALAGKPLHTA"
     gene            27397..>28205
                     /locus_tag="VF195_13935"
     CDS             27397..>28205
                     /locus_tag="VF195_13935"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein T"
                     /protein_id="HEX6581948.1"
                     /translation="MAHARPADPADEAEAVSDELKRTPLEAEHLRLGAKMAPFAGWQM
                     PIEYEGALAEHRAVRSHVGLFDLTHLGKVEVVGAGALGMLQRLVTNDLSRARVGEALY
                     NLVLNEGGGVIEDLIVYRLGEERYFVVPNASNAPRVLRMLEEEKSDEPLHLMYHQDWC
                     FLAVQGPEAMSAIAATFPTAPELAFMRCMETEYHRRPVIVTRSGYTGEVGFELFTYQD
                     VAQQLWDELLGSVRPLGGAPCGLASRDILRLEMGYALYGQDLFEASTAFEAG"
BASE COUNT         4099 a         9725 c         9875 g         4506 t
ORIGIN      
        1 cggctccccg gctccttcgt ccccgcctcc tgagtcctcg atgccaccgc cgccgttgcc
       61 accaccgccg ccgttgccac caccggcgtt gccaccgcca ccggcgttgc caccgccacc
      121 ggcgttgcca ccgccaccgg cgttgccacc gccaccggcg ttgccaccgc cgtcgatccc
      181 gccgatgtca gttcctccgc caccgttgcc gacatcgtcg acaccggcac ctccaccggc
      241 gtcgtggccg acattcccat tgccgtcctc gctgctccca tcctcgctcc cgttgccagc
      301 ggtcccttca ccgctcccgt tctccccatc cgtgttctcg tccgcgaccc cccgctcggg
      361 tgcgccgccg accgttccgc cgttccctcg cccgatccct ccgccgaccc cggtcacgcc
      421 gtcatcggac ggctcttcat ggtcgtctgc gagggtgtcc tctccggtca caccctgacc
      481 gggcgtcgcg aacgactgtt ccgttcccgg agacggggcg gacgctgccg agagtcgctc
      541 gcgcgccggc acgaacatcg cgaacgcctc ggaggcgaga tcgcgagcgc cgtcggggag
      601 atgacccgtc atcgccaggc tgctgccggc cgtgatgacg atcgtggcgg cggcgaccgc
      661 tgcacgggtg acgggacggt gcacggtgcg ccgccgcgcc ggctcggcga tgctccggcg
      721 cgtggcgcgg acctcagcgg ccatcgaggc cacggtggag tgctcggccc agagctcaga
      781 cgggagcggt cgtgccccga cctccttgag cagcgcatcc aggtcgacca cctgcccctc
      841 gggcttgcgc atcatggcgc gcaggagcga cttcccgtcg tcgcgaggcc gagcctcgtg
      901 catcagccca cctccgcggt ctcggcctca tcggcgtcat cggcgtcgcg caggagtcgc
      961 agggcatcca tctcgaggag ccgcacggct cccgtgtcct gcccggtgag ggctgcgacg
     1021 tcgtccacgt cgagatgtcc cagcccgcgc aacagcaaga cgtcggcgtg atccggcggc
     1081 agcgctgcaa ccctcgacag cgcctcggac atcggctcgc gcgtgcgctc ctcgagctcg
     1141 atggggccca cgacccgatc tcccgcctcg agggtgcgac gggcgaggtc gaacagccag
     1201 cgccggaacg cgatctcgtc gcccgagaag cgctggagac cctgagcgac ctggatccac
     1261 acctgctctg ccgcgccctc gggcgcgctt gcgccacggg ctcgcaggta gcgcaggacc
     1321 gcgggatgga actctcgata caacaggccg atcgcccact cgaccccggt cgtcgccgag
     1381 gtcatgaccg tctcgaatcg ctcgcctcgg aggccgaggg tcggcgtctc aagggatcgg
     1441 gccccaggcg gcaccgcatc gaccggttcg cggcggcgct gtgtgggttg cgtgacgatc
     1501 tgtgggtcct tcgccaagcg gaaagagaac cgtgaccctt ctcccacgcg actctccacg
     1561 tcgagacggg acccgtggag ctggaggatc tccgagacca ccgcgagccc gaggcccgtg
     1621 ccacgggtgg accgagtatg cacgtcgcct ccgcggaaga atcgctcgcc gatcctcgcg
     1681 acctcgctct ccgggatgcc gggcccgtcg tcgctcaccg ccacgaccac gtccgcacct
     1741 tcgtcccgcg cgctcacgac cacccggctc cccgcgggcg tgtacttcga agcgttcgac
     1801 aagaggttct cgatgatgcg ttcgatcagg ccggggtcgg cctgcgcgca caacgcggac
     1861 ggaacgtcca gctccacccg atgtctggac aggagcgtgc tcaggcgatc gacgaccgcc
     1921 gggaccagtc tcgacagatc gacgtcctgg gcgtccgggc ggagctggcc ggcttcgagc
     1981 ttggagaagt ccaggagctg ggcgatcacg tggtcgaggg tcgcggcatt ggcgttcagg
     2041 cgggtcagga agtccgcacg gatctcgtcg tcgatcgtcg cccactggtg ttcgagtgtc
     2101 ttgcccatcc ccgtgatcac ggtgaggggc gtgcggagct cgtgcgagac cgtggagagg
     2161 aagtcgttct tcatgcgatc gaggtcggcc agccgctgga tgacctggcg ctgttcgtcg
     2221 aaccgacgca cgttctcgag cgccaggctc gcctgcgacg acagcatccg gaacacctcg
     2281 acgtcctgtg tggaagggcc gggagcagcc tccgtcccga cgacgaggac ggctgccacg
     2341 ccgtcgctca ccgcgatcgg cacggcggcg ccgaacaggg gcagaccgat cgtccgtcct
     2401 cggaacaccg cgaagacgcc ggtctccgcg gtcgtgcgca cgacctcggg aacgctgctc
     2461 tcgggtgcgg gaacccccgg caccgtgtgc caatcgtcgc cttcgcgcac gaagacgcgt
     2521 gcgaacggga agcccagccc gccgaccgct tcgatcgtgg tctcgaggac ctgatcggcg
     2581 tccacggtcg agctactcat gtcgcgagcg gcggccgaga cggtcgcaag cagggcccat
     2641 cgacgatccg cctcggcgcg ggcatcccag gccgcgcgga cctggacgcg gatctcctgg
     2701 acaaggaacc cggccaggaa cgtcaggacc gagaggacga tcaagggcag cggaccctgg
     2761 tcggccgcgc ggaggacgat cccgtacgcg acgagggtca tcgcgagcag tgccacctgt
     2821 ccgcggggcg agaaggcaac cgcgaagaac acggtggtga gcgcgtagag caggacgagc
     2881 ggcgagtgtg tccctcctgt cacccacaca ccgacggtga ccatcccgag ggccgtgacg
     2941 gcccacaccg ccaacaggcg catgcctccg cgcgtcgcca ggagccgatc ccacgggagc
     3001 agggtcgcaa gcgcggccgc cacggcgagc ccggtcagca ccaggccata accaccgagg
     3061 tcgagccgag ctcgggtggc gaggacgaac atcgcggcag ccgcgagcgg ggcaaggagg
     3121 gtgacccgca tcccaacgcg gagcgtccgg aactcgatgg ggagggggcc tcctggtgaa
     3181 cccgtcatgt tcgttgcctc cgagcggcga ctacggcgcg gcatccccgc gcgccgctgt
     3241 agggcgcggg atggcacgac ggtagccgga tggccgacag tgattcatcc gtccctcggc
     3301 ccaatacgag agtgagccgg ccggtcgggt ccgagccctt cgacctaggg acgcccggaa
     3361 cgaacctcgg cgaccgttcc tcgacggaga ctcaggggga ctccggatcg atcgagccca
     3421 acgagcgatc agccgatcga ctcggagagg ttctcggcgg attcctgcga gatcccgagc
     3481 cgctcggcga gggcaggcgt cttctgatcg agcagtctgc ggatcgactc cttcgacaac
     3541 ccgagctcgt tccggatctc ttccgcgctg agtcccgcct tggtggccag atcgagttgc
     3601 tcctcgatca tcttctggcg gcgctcggcg acctcctcga gaaggacgag cttctcgatc
     3661 tcttcacgga gcaaggagat gtccatcgag gagccttcca cggatcagct cgattcgtac
     3721 ttgaacgaga tgttcacgcg ggcgcgatag ctggtgactt tgccgtcctc gatcgtgaca
     3781 tcgaacttca cgacttcgcc gacgcgcaga tcccgcaggg tggtggccgc cgtctcgaca
     3841 gcgttcttgg cggcttcctc ccacgacgtg gagctcgtcc cgatgacctc ggtgacccgg
     3901 tagacgctgt cggtcatact ctcctcctct gcacggctct gcaccctctg ggggcatccg
     3961 gtcctctggc gaggctcgca caggctatcg tgcctgcgtg atccgtgcgc ttccgctgat
     4021 cctgctcgtc gttctggtcg tggcgctcgc gatgtgggtc cgctcctgga cgaaagcggc
     4081 ggcgacgcgg cgccccgcac ggcgaggcag ctcccggatg gcggctacga cttcgaaagc
     4141 aggatcgagg gtctgaagcg ccgtcgtcgg agcgcacccg accccttcga acggcatcag
     4201 gggccagata tgagtatggg ggagttcctg aacacccgct ccgaagtcga ggcgtacgtc
     4261 gagcccgaga ccgtggtcag cccgcgatcg gtcgtgctgg tggacggagc gggcgagtgg
     4321 cgccggttcc ggccgagcga ggactccgtc ctccgacgcg tggccgggga acgcgggatg
     4381 ctgatcttcg atgcctcccg cacggggtac ccgtcgcgga tgcgacgcgg tccggggccg
     4441 gcgacctagc gacgcagctc gaaccaggcg acgcggtgga gccggccgcg gatccggaga
     4501 agcacctgac ccaggaggag ctcgtcgtcg atccggacga gctcgtcgag gatgtctcgc
     4561 acgatccacg gactctgcgg atgctcgtag tcgatcttga agacgtcggc gccaccgggg
     4621 gtgtggacgc tggggccgtc ccacgtggtg aaggggaagg tggcgtaccg gcgatcgccg
     4681 agcggccagt cgagatcata gccgggccac agggtccgct ggaccacgcg gaacgacgtc
     4741 gagaacgcgt tgcgtccctc cttggcctct tggtcgaacg tcttcccctt ccacggcatc
     4801 cagacacgcg cgagtccgac gatgatcggg tccaggccat agccgacggt ggtcgtgatg
     4861 acgcggccag gcatcagacc gtcgagcggc tccggggtct ccccgttccg gaagcaggtc
     4921 tccaacgcgg cgagcgccgc cggtcggtcg tgtggcaggt cggcgagggc tcggaggtac
     4981 cgcagcgtga gctctgaagg catcatcgag tatcgcacgg tcctcggcga acccttccgc
     5041 cgtacccttc gtaccgtgag ggtgcgtcgc gcgctcggac tgatcggagt ggcggcgctg
     5101 accgtcgcct gcacgcaagg gggatcagcg cagccttccg ggacgcgcgc gccgagccgc
     5161 tcgtcagctg tcaccccaga cccgggaccg atccgggcag agcgcttgct ggtgctgacg
     5221 gatgacggca acgtggtcgt cgtccgcgcg gacggcgggg acccgcgtcg cctgacggag
     5281 ttcgactcgg atggcgcgac ggtcgaggtg cgacatccgg tctggtcgcc cgacgggcgg
     5341 tcggtcgcat gggccgagct cgagatcggg gatcagggcg cacgcccgcg catcgtcagc
     5401 atcggatcgg acgggtcgcg gcgcaccgag ttccccgtgg acactggggc gttcttcctg
     5461 caatgggacc ccacgtcgtc ccgcatcgcc tacctcggga acttccaggg cagcgtgggg
     5521 atgggcgtcg ccggacgcgc cccgatcggg gaccccgtcg ccacgacgct cggggtcggc
     5581 cgacccttct acctgtcctg ggcgcccggc ggcctcgagc tgctggtaca cgtcggtgac
     5641 gagacgctcg ggcgattgga tctcgaaggg gagctgcgcg acgtcggcga tgcgccgggc
     5701 atcttccagg ctcccgtctg gctcgcggac gggcggatgt tctacgccac cgccgagcgg
     5761 gatcgacaga cgctggtggt gcgcgacggg gaccgactcc gagagctggt cgagttcgag
     5821 ggcgcgatcg agttcgtggt ggatccccgc ggcgagcgga tcgcgtaccg cgtggacgac
     5881 ggcgccggac cgggcggggt ggaggtgatc gaaacgacga cggcgcgttc gcaggtcgtc
     5941 tcggcgtcgc cgacgttcgc gttccagtgg agtcccgacg gcgaacggct cctgctgctg
     6001 acgccggacg aggacggcga gaccggcgct caccgatggc tggtgtggga cggtgacgtg
     6061 acgcggccgg tgggcccggc gttcgtgccg agcccgtcct tccttcggga ctacgtcccg
     6121 ttctacgggc agttcgcgca ggcgatgacg ccctggtctc cggacggcgg tgcgttcgcg
     6181 ttcgcggggc tgatcggcga tcaggccggg gtctgggtgc aggatttccg cgccgccgat
     6241 cccacgttcg tactcgaagg cggttcggtg gtggcgtggt ccccgacgcc gacgtgccgc
     6301 ccgaccgtag actgcgcgtc cgtgttgagt gagacgaccg tgttcgacgc cgccgggggc
     6361 atgccgttct tcgagcagct cgtggcacgg ttctacgaag gtgtcgcggg cgatcccgta
     6421 ctccggcccc tgtatccgga cgatctcgcg ccgtccaccc ggcacctgac gctcttcctc
     6481 gcgcagtact ggggcgggcc caccacgtac gacgaagaac gcggtcaccc tcggttgcgg
     6541 atgcggcacg ccccgttcgc ggtgggcccc gccgagcggg accggtggct ggtgcacatg
     6601 cgggcggccg tcacgacgat ggcgccgccg gagccgatcg agagggcgct cgtggactac
     6661 ttcgagatgg cggccgaggc gatgcggaac cgggactgag cgttcaggcg agcagatagc
     6721 gccgcgacag ccgcacctcg gtcacgggat cgatccgcag cacccgcccg tcgagcgtca
     6781 ccgttccgtc ggcggccgaa acctcgatcg agggaacggt gcggttccgc gcgaggccgt
     6841 cgcggcggat cgcgcgggtc ccccggacgg ccacgagccg ccgtcgcgtg ccgagtgtcc
     6901 gggcgatccc cgcgtcgatc gcggccgccg agacgaacgt ggtggagacc cgcgccggcg
     6961 caagccccgt gccaccccaa tcgggtccgt atcgggttgg ctcggcgccg tgcacgctcg
     7021 cgttgccctc gccgatcgcg gtccacgcga cgacccctcc tttgaacacc atcaccggct
     7081 tgaccccgaa atgcgacgcc tcccacagga ccaggtcggc gagccgtccg gaggagaggg
     7141 agccgacctc gtccgagatg ccgtgcacgc gggccggctc ctcggtgacc ttcgacaggt
     7201 agcgcaggac acggtcgttg tcatctggct catcgcggcc gctcccgccc gcctccgtgg
     7261 tcgccgccgc caccttcatc gcatgggcga gctggaacgt tcgtcgcagg gtctcgccga
     7321 tccggcccat cccctgcgag tccgagttca cgatcgagat cgcgccgagg tcgtgcagcg
     7381 gtccctcggc ctccatcgtc gcgggatgga cgagctcgcg agccgccacg acgtcgtcgg
     7441 ggagcgccgg gttgccctcg tgcacgatga gcttcatgtc ctgctgctcg gccgcggtcg
     7501 cggcgccata ggggatcgtc ggtgtggtcg acgagcagaa gacgttcggc tcgcggacga
     7561 tcccgatcag gtccgggatg tggccgcccc cggagccttc gacgtgatac gcgtggatcg
     7621 cacgaccggc gatcgcgccc accgtgtccg cgagctcgca cgactcgttc aacccgtccg
     7681 tgtgcaggca cacctggacg tcgtgcgcgt ccgcgacccg gagcacctcg tcgatcacct
     7741 cggggtaggc gccccaatcc tcgtggatct tgagtccgac ggcgcccgcc tcgatcgcca
     7801 cctcgagctg cccggggacc cccgtccggg ccgacggctg gagcccgatg ttcacgggga
     7861 ggagctcgaa ggcctccagg gtgcgcagca tccgccacgg gggttcctcg aacccggcgg
     7921 tgatgagcgt ggtgacgccg gccgagagcg cgaccgggac gagcctgggg gagagcaggt
     7981 gcacgtgtga gtcgacggcg cccggcgtgg cgatcagacc gtggcaggga accggccatg
     8041 tgttcggccc gatcgtgaga tcgacgccgt cggtgatgtc cgggttgccc gccctcccga
     8101 tcccggcgat ccggccatcc ttgatgccga tgtccgcctt caggacgccg aggagcggat
     8161 cgagcacgat cgcgctcgcg atgatcgtgt cgagctcgct ctcagtggtc gcgcggtcgt
     8221 gctggaccat ccccgagcgc cagttcttcg cgtagcccca gagcgcctgg tcggccgggt
     8281 cggtgaggtc acgctcgatc cggatccaga ggtcggtgtc gcccagccgg atgcgatcgc
     8341 cggcggtcgg accgtggcgc ctcgcgtgct cggcgcgcga gtaccgctca ggcacccgcc
     8401 ggagcccccg tccctccgta gcgcacgagg tccacctcgt gcgtctcgcc cggcgcccac
     8461 cgcaaggagt cgcccgcggg caggtcgagc cggaaaccgt cggccgcctg gcggtcgaac
     8521 gtgaggtgct cgttcgagcg ccagaacggg aagtgcgagg acacacggat cgggtgctgg
     8581 ccgcggttcg tcacggtcag atggatccga tcacgccccg ggccgagctc gacgtcgccg
     8641 tcggcgaacc ggatcgcgcc gggtccgtcg gacccagcgg gcccgaacgg ctcacggatc
     8701 acgaacagcc gtgtgccatc ctcgagcagg atctccacgc ggatctcctg cacgatcgcg
     8761 gcgatgccct ccatcacctg ctcgggcgtg acggcgtccc ggccggccgc gacgacctcg
     8821 tcgaacgagc tccccccgcg gcccgccgcg tgcatctcgt cgcacacgag cgcgaccgcc
     8881 tcgggcgcgt tcagccgcag gcccttcgcg agcgtcgagc gggccagctg ggcgacggtg
     8941 aagacccgca gtcgctcctc ctcggtgggg gtcagttgca tcggctcctg tccttctctg
     9001 gctcgctcct ctggctcttg gcgagcgtac ccttggtcgc gatgacacgc cctcccgacg
     9061 agcgatcgcc cgatccggcg cccaggacac cacccacgtt ccgtgatgcg tcgcgcttct
     9121 ggatctggct cggtttcgtg aacttcggcg gacccgccgg gcagatcgcg ctcatgcacc
     9181 aggagctcgt cgagcggcgg cggtggatcg acgagggacg gttccttcac gcactcagct
     9241 tctgcatgct gctgccgggc ccggaggcga cgcagctcgc gacgtacgtc ggctggctgc
     9301 tgcatcgcgt ccgcggcgga ctgatcgcgg gcgtcgcgtt cgtgctcccg agcttcttcc
     9361 tgcttctcgg gctctcctgg ctcttcgtga cgcacggcga cctgcgatgg gtcgcgggcg
     9421 cgttcgcggg gctcgcggcg gccgtcgtgg cgatcgtggc gcacgcgatg gtcaggctgg
     9481 cccgcaccac gcttgcgaac ggcctgatgg cagcggtggc cgcgatctcc ttcatcgcga
     9541 tcttcgtcct cgatgtcccg ttcccggtgg tgatcggggc agccgtcctc ctcgggctcg
     9601 tcgggggtcg gatccgtccg gcgctcttcg cggtcggcgc cggcaaggag ctcgaggagc
     9661 gcgccgccgc gtccgtggtc ggcacaaccg acacccctcg cccgacgtgg cgccggagcg
     9721 ccgtggtgct cgtcgtcggg ctcgccgcgt ggtggctccc gatcgtcgcc atggcggtat
     9781 ggcgggggga cgcggggacg ctcaccgaca tgggttggtt cttctcgaag gcggcgctgg
     9841 tcacgttcgg cggcgcgtac gccgtgctcg cctacgtgga tcaggccgcc gtcggcgtgt
     9901 tcgggtggct ccggcccggg cagatggccg tggggctcgg cctggccgag tccacgcccg
     9961 gtccgctgat catggtcgtc gagttcgtgg ggttcgtggg ggcgtaccag catccgggcg
    10021 ggctgccgcc gttcgtcgct ggtgtactcg gcgcgaccgt ggccgtgtgg gcgacgttcg
    10081 cgccctgctt cctctgggtg ttcctcgggg cgccctcgat cgagcggatc cgtggtgacg
    10141 ctcggctcgc atcggccctg cagacggtga tggccgcggt ggtcggtgtg atcgcgaacc
    10201 tggcggtgac gttcgccgtg gtgacgctgt tccggtcggt gcgcacggtg gcgttcctcg
    10261 ggggcgagct cccggtcccg gtctggagcg aggtggacct gttcgccgtc gccgtcgccg
    10321 ggatcgcgtt cgtgggcctg tggcggctgc gctggcgggt cgtccccatg atcgtcgcga
    10381 gcgctgcggc gggcctcgtg gtcaaagggc tgctgtaggt gagcgctccc gccgcaccag
    10441 gatcgcggcg ccggccacgg cgacggccgc gaagcagaac cactggatcg cgtacgacag
    10501 atgcggcccc tcggacagct cgggaagggg gaccggcacc gggagttccg tgttgggtgg
    10561 gtcttgggct cggagctgga ccgcgaacgg ggccaggtcg aacgggagtc ggtcgccgat
    10621 ccccgagatg tcgagcgttc gaacgatgcc gtcgcggtcg ggccggcccc ggcctcggct
    10681 ctccggcgcg atgagccaac cgtcaacgac cacgtcccga acggccggtg cagcctcgac
    10741 gacgggagcg cgatcgagcg agaacggcac ccagcctcgg atgacgagca cgccggagcc
    10801 gtcgtcgagc gccaacggag tgacgacgaa gtgccccggt tcgccgccga gggaccgtcc
    10861 gtacaggagc acctcgccct ggatgtcgta cgtaccctcg acgacgaccg agcggaaggc
    10921 cgacagtgcc ccgaagtcgt ccgcggttcg gaccgtggtg gggggcgccg atcgggctgc
    10981 ccggatctcg gcgttcagcg cgcgacgatc ggcgaggcgc tggagctgcc agaacccgag
    11041 ggcgatgcag accgccgcca cgatcgtggc gatcgcgacg atcaccactc gacgccggcg
    11101 ggacggatcc atcggggtcg ggcgctaggg gcccttcgcc tcctggatca cgttcccccc
    11161 gtcgaccacg acggtctggc ccgtgacgta ccctgcccga tccgacgcga ggaacgcgat
    11221 cacctcgccg atctcctcgg cggtccccga tcgtccgatc ggggtgtggt gaccgatctc
    11281 gtgctcttcg ggggtctgcg agcccgtcgc gacgaagccg ggctgcacgc tgttcaccgt
    11341 gaccccgcgc gacgcgacct cgatggcgac acctcgcatc aacccgtcca tgccggcctt
    11401 ggccgcggag taggcggtca ggcccgggta cgtgacgatc ggccccgtca cggacgagac
    11461 catgacgatc cgtccccaac ctcgctcgac catgccgggg aggacccgtt tcgtcacgtt
    11521 gtaggcggta tcgaggttca tcgcgaggtc gcgatcccac gcggcatcgt cgatgtcgag
    11581 gaaggctccg tccgatgtcg catccatcga gaccgacacc atgccggcgt tgttcaccag
    11641 gatgtcgatg cgaccggatc gatcggtgac gtcgtcgacc atccgccgca cctgtgcgcg
    11701 atcggtgagg tcggcgacgt aaccgttcac ttcggcgccg gttccgcgga gctcggccgc
    11761 gcgatcgtgg atccgatcgg tcgtggacgc gatcgccagc gccgcacctt cgcgggcgag
    11821 gatcctcgcg gtggcgaatc cgatccccgt cggacttccc gcgccggtca ccagggccac
    11881 ccgtccgttc gcttctccac gcacgacccg agcgtatcgg ctgtccaagg tgcgaaccga
    11941 aggcatcggc taggcttctc cgctcatgga ccccgcccgc ccgttgctcg agatccagga
    12001 gcacgacacc gcgatagacc ggctgcaggc gcggatcgcg gccctcgagg cgggcgcgga
    12061 tgtcgccgcc gcactggccg aagcgaacga ggccgagcgc gtgatgggcg agctccggtt
    12121 gaagctcgac gagctctcgc gtgatcagca gcgcttcgag cacgagatcg actcgatgtc
    12181 gcagaaggcg gccgccgagg agcgacggat gtacgacggc tcggtcgcga acgctcggga
    12241 gcttgactcg atccgtcacg aggtagacaa cctcaagaag cgccggaccg atcgggagga
    12301 cgagctgctc gggctgatgg aggtgcggga ggagctcgag gcccaggagc gcgaggcgga
    12361 cactcgatcc acggcgctgc gggcgcgggt ggacgaggtg ggaggcatcg ccgccgagga
    12421 gctcgggacg ctcaccgacg aggtgaagca acggaccgag aagcgcgctg ccctcgcgga
    12481 ggcggtcgac cccgagtacc tggagctgta cgacgacctc cggccccaga agaagggtgt
    12541 cgccgcggtc gagctcgtcg acggcgtgtg tctcggatgc cacgagcagc tgtcgtccgt
    12601 gtaccgcgac cgactcaagc gcgtcgaggg cccgaagagg tgcgagcact gtcggcggat
    12661 cctggtcatc tgatcgtctc ctgcgacggg gcctcgcgcg ggaacccggg gcccgccggt
    12721 atcggcgctc aggtcaccga cgagagcggc gccatcctcg gagagatcgc ggagggcatc
    12781 ggcgagacga cgaacaacgt ggcggagtac acggcggtga tcgagggtct ttcgctcgcg
    12841 caggagctcg gggcgaagac cgttacgctc agatcggaca gcctgctcct catcaaccag
    12901 ctcaccggac ggtaccgggt gaagagcgag cacctgcagc cgctgcaccg ccgcgcgcgg
    12961 agcctcgggg ccgggttcga tcgcatcacg ttcgagcacg tgccgcgcga gcggaacagc
    13021 gcggccgaca ccctcgcgaa cctcggcgtc gatcggtggc tcgcggggga gcggtagcgg
    13081 taccctggat gcggaggagc cggccgggcg gccgcgggcg cgcatcgcgc ggtcgaggaa
    13141 agtccggact ccgcagggca gggtgctggg gagacccagg cgggggtgac ctcgcgaagg
    13201 cgcagcagaa agcagaccgc ctctctctga tcgtcttggg gagaggtaag ggtgaaacgg
    13261 tgcggtaaga gcgcaccagc ggcgcgggtg accgcgccgg ctcggcaagc cccacccgga
    13321 gcaaggccaa gcaggcagcg ttcgagggcg gcccgcccga gctgccgggt aggccgcacg
    13381 gcgcgggtga accgcgacgc ggcgccgcgg cggggagacc cgcctgcgcg ccgagagaga
    13441 tggccgccgc cgggacctcg gtcccggcac aggatccggc ttacaggccg gctcctccat
    13501 tccatgtcgg ggctacgttc gctccttcgt cgctcactgc ttgagccccg gacccctctc
    13561 acatcggggc tacgttcgct gcttcgtcgc tcactcattg agcccgggac ccctctcaca
    13621 tcggggctac gttcgctcct tcgtcgctca ctcattgagc cccggacccc tctcacatcg
    13681 gggctacgtt cgctccttcg tcgctcactg cttgagcccc ggacccctct cgtccgtcgt
    13741 gtagcgtcct tgcgatggac atcgagaagc tgcgacggat cctcctgttc gaggacctgc
    13801 acgatcgcgc gctggagcgg atcgcggagc tttcgaccga gttcgaggag cccgctggat
    13861 gggttctcgt cgaggtgggt cagccgggtt cggggatgtt cgtgctcgag gagggaaccg
    13921 tcgaggtcca gacccccgac gggcgtgcct gggagctcgg accgggggag ttcttcggcg
    13981 agctcgcgct tctcaccgat caacctcgga acgcgcgcat ccgggcgaag tcacccgtgc
    14041 ggtgcctcgc gatctctcgg atggacttcc agaagctgct ctcggaggag ccggagatcg
    14101 cggtggcgat gctccccaag ctcgcccaga gggtggacgc cccgcactga cgggccgacc
    14161 tggcgctggc cagaatcgaa catacgttct atcctggccg gtccatggat cagctcttcg
    14221 agatcgcccg gaagacgttc gacacgcccg agttccgcgg catgacgttc atcgaatccg
    14281 aggcgaagtc gatcatcaac cgtgtgccgg gcggcgccct gcccttcgac tggacgatca
    14341 acccgtatcg gggatgttcg catgcatgcg tctattgctt cgctcgaccg acccacgcgt
    14401 acctcgacat gaacgccggc cgtgacttcg agacgaagat cgtcgtgaag gtgaacgcgc
    14461 ccgacgtcct gcgccgcgag ctcgccgcga agcggtggaa gggcgagcac atcgcgatgg
    14521 gcacgaacac cgacccgtat cagcgggcgg aaggaaagta ccggctgatg cgcgggatcc
    14581 tcgaggcgct cgtcgactat cgcaacccgt tctcgatcct gacgaagggc acgctgatcc
    14641 ttcgcgacgt cgacctgctg cagcgagcgg ccgacgttgc caccgtctcc gcagcgttct
    14701 cgatcggcac gctggacgag acggtctggc gtgagacgga gcccggcacg ccccatccga
    14761 aggcgcggat cgacgcgatc cggacgctca ccgaggcggg tatccccacc ggcgtcctga
    14821 tggcgccgat cctgccgggc atctcggatc gccccgagca actccgcgag ctcgtcgagg
    14881 cggcgatcga cgccggcgcg acgcatgtct caccgatcct gttgcacctg cgccccggcg
    14941 tccgcgagga gttcctcccg tggctggagg acgcgcatcc ggatctcgtg ccgcgctatc
    15001 tcgagatgta ccggcagccg tacggaccga aggctctccg cgagagtctc ggtcgccggg
    15061 tgtcgtcgat ggtgtccgct gcgggtggga tccgtcggag tgtggagccg gcgcgtcggt
    15121 tcttccgacg cgaccgcgaa aggccacggg accagagggc cgagcaattg cggctgctct
    15181 agcgcggatg ggtccgggcg acccgtcgtg ccgaagggcg gaagtgagag gatggacata
    15241 cgaatcgagc gggaccgctc ccgaagaggc cgagggcagc cccccgaatg agggagggca
    15301 ccgaatgaga agcaccttgc gagatcccac ggctcggctg ctggaagccc tgtcccatcg
    15361 gttgaggccc cggatgcgga acgcgtcccc acgggtcccg aatgggttca cgctgcactc
    15421 ggatcgtggg agaggatcat tccggcgaga tcgcaccgcc ggggaccgta gcgtcctcgc
    15481 agactgagca ccgtccacca cgagtggaac cgcccaagac ggcgcgaaga cctcgcgacc
    15541 ttgccttccg cggttcctct tcttgagatc gggaggttgt catggctcat tggccaagga
    15601 ccgttacagg caagtcgttc tacgtcgcac tgatcgcggt cgccgtaccg ctcaccgtct
    15661 ggttcctggt gacggtcccg agctacgtga tcttggcgct cggcggcatc gcgatcatct
    15721 ccgccgctct ctacctctgg cggcgcgggc tcgatgcggc tcgcgagaag gcctgggtgg
    15781 gtgagttctc gttcggtgac gtcgtcggtc gcatgaaggc cagagacgcg ctcgatcgcg
    15841 cccgggagcc cggcacgctc acggccgccg ccgtgtcaag cgggggctga gaccccggat
    15901 tccaccgcgc gccgcaactg cttgccggcg tggtagctgg agcggaccaa cggtccggcc
    15961 tcgacgtggg cgatgcccat ctcttcgccg atccgcttcc actccgagaa ctcgtcggga
    16021 tgcacccagc gatcgaccgg caggtggaac gttgtcggct gcaggtactg gcccatcgtc
    16081 aggatgtcga tgcgcgcatc gcggagatcc tgcatcgcgg ttgggatctc ctcgggccgc
    16141 tcgcccatcc cgaggatcag gttcgacttc gtgacctgga cggcgcggtg ccgcttcgcg
    16201 aagcgcagca cctcgagtga ccggtcgtaa ccgaacgccg gacggatccg gtcgtggagc
    16261 ctgggcacgg tctcgaggtt gtgggcgaac acgtccggct cggcgtcgag caccgtcgcg
    16321 atgtcgcgct cgccgccctt gaagtccgac ggcaggacct cgatgccgat ccccgatacc
    16381 gcctcgcggg cggcacggat cgctgaggcc cagatccgcg ccccgccgtc ctcgaggtcg
    16441 tcccgggcga cgccggtgag cacgacgtag cgcagcccca tctcgccgac ggcctcggca
    16501 atcctcgccg gctccccttc gtcgaccggg tccggcttcg ccgtcatcac gtcgcagaag
    16561 ccgcagcgtc gcgtgcactt cgatcccagg atcaggaacg tcgcctcgcg ctcctcccag
    16621 cactcgcgga tgttcgggca catcgcctcc tcgcagacgg tgtgcagcga gcgctcccgc
    16681 atgatgccct tgaggtcctc gtagttggga ccggtcgtga gccgcacctt gagccacggg
    16741 ggcttcctgc ctgtcacgga tccgacgccc gggccgttcg tgacacccac gatcgggagg
    16801 tggagccgcc gcttgttcgg attgtggtcg gccccgtgcg ccatggatgc catcgctccg
    16861 agtgtagcca ccgtcccgct atgagcgggc gagctcgcgc atccgcgcgt agatgtgatc
    16921 cggttcgggt tccatgaagt agtgctcgag tggcgccgcg aacggcatcg cggggatgtc
    16981 ggggccgccg atacgcagga tcggcgcgtc gaggtcgaac agggcctgct cggcgatcgt
    17041 ggaggcgatc tccgctccgg cgccgtaggt ccggttgtcc tcgtacacga ccatcgcctt
    17101 cccggtcttc ttcacggagc ccacgatcgt gtcgatgtcg agcggcgcga gcgtccgtgc
    17161 atccacgacc tcgacggaga agccttcgcc ctcctcgagt cgctgtgccg cgtccaggca
    17221 gaggtggagg gagtagccgt agctcacgca cgtgaggtcg tcgccccctc gcttgacttc
    17281 cgcgaccccg atcggcgtga ggtaggcgcc gtcgtccacc tcgcccttga tcgcccggta
    17341 ggtcttcttg tgctcgagga acagcaccgg gtccgggtcc ttgatcgcac tgcggaggag
    17401 cccggcggca tcggccggcg tggagggcac cacgaccttg atcccgggga tgtgggcgta
    17461 gaacgcctcg atcgactgcg agtggtacag cgccccgtgg atgccgccgc catacggtgt
    17521 ccggatcacc atcgggacgc cgaaatcgcc gttcgagcga tagcggaggc gggcggcttc
    17581 ggagaccacc tggtcgaacg ccgggtggat gaagtcggcg aattggatct cggcgaccgg
    17641 cagcaggccg tgcagcgcca tcccgatcgc gatgccgacg atccccgact ctgcgagtgg
    17701 cgtgtcgatc acccgctgct cgccgaactc ctccatccag ccggcggaga tccggaagac
    17761 accgccgcgc gcaccgacgt cctcgccgag cagcacgatc cgctcgtcgt tcgccatctc
    17821 gtcgtggagc gcgtccctca ccgcggtgac cacgttcttc tccgtcaccg ctcgtcctcc
    17881 tgctcgcgga acacgtggtg cagggcggtg tcgggatcgg gatccggcgc atcccacgcc
    17941 gcgcggatcg cgtcgtcgat ctcctgcttc acctcggcgc ggagcgcttc ctgagcggcg
    18001 tcatccagca gatcgtgctc gcgcaggtag ccggcgaact ggatgagcgg gtcccgggcc
    18061 ttcgccagct cgacctcctc gcgcgagcgg tagctcttgt cgtcgtcgtc ggaggtgtgg
    18121 ggcatgaatc ggtacgtctt gcactcgatc aacgtgggac cctcgccggc tcgcgcccgc
    18181 tcgtgtgcgg tcttcatcgc tccgtagcac gcgaggacgt cgttgccgtc gacgacgacg
    18241 ccggggaagc cgtacccctc ggcacggctc gccacgtcct tcacggccat ctgcttggac
    18301 tgcgggacgc tgatcgcgta ctggttgttc tcgcatacga agatcaccgg aagacggtgg
    18361 atcccgctga agttcaggcc ctcgtgccag tcgccttcgc tcgtggcgcc gtcgccgaac
    18421 cagcagccga cgacggcgtc ttccccccgg tacttcgcgg cgtaggcgat gccggccgcg
    18481 tgcggaacct gcgtggcgat cggggacgag ccgctgatga tgccgaggcg tcgggacccc
    18541 cagtgcgccg gcatctgtcg gccacccgaa gatggatcgt cggccttcgc gaagacgccg
    18601 aggaagacct cgtacggcgt catcccggcg ctgaggacga tgccgaggtc ccggtagtag
    18661 gggagccaga tgtcggtgcc gcggcgcatc gcccaagcgg tcccgatctg gcagccttcg
    18721 tgtcccgccg ccggcaccac gaacggcgcg cgacccatcc ggttctggcg gagcgcggct
    18781 tcgtccagca gccgcgacgt gaggagcaac cggtgcatcg acgcaaggtc gtcggccgac
    18841 aggccgagcg cgccgtgtgg ggtttcggct acccgggtgg acacgccgtg tctcctcgca
    18901 ccgtcctcgc aggataacag cggtctcctg ggggaggccg aacgcgcacg ataccccctg
    18961 ggggtaccac gaggggcccg gaccgccgcg gactgcggcg gaaggagggt gccgtgtacg
    19021 gctatgccag gaacaaggac gatctgcaga cccgcttgcg ccggattcga gggacaggtg
    19081 cggggcctgc aacggatggt ggacgaggat gtgtatctca tcgacatcct gacgcaggtg
    19141 aactccgtga ccgcggccct caaggccgtc gggatgggcc tgctcgacgc ccacgtccgt
    19201 catcgcgtga cggagagcat cgaggcgggc gacggagagc agaaggtcga ggagctgatg
    19261 agcgcggtcg cgatactccg gccgctgctc ctcggcggtg ccgcgaggaa cagaacggga
    19321 ggtcgggcgc gttgtcaccc acgtggccgt cgtcgagact ggccggagtg acgccaggtt
    19381 cgcgaccgag gacacgagga gtgggaacag cgagatgaca gcatcgacca acgtgatcga
    19441 ggtgacggat cagaccttcg agcaggtcgt cgtggagggc tcgaaggagc ggccggtcgt
    19501 cgtggacatg tgggccgcct ggtgtgggcc gtgccgccag ctgacgccga cgctcgagaa
    19561 ggtcgcagac gagcgctcgg gagccttcct gctcgcgaag ctcgacaccg atgcgaaccc
    19621 agtgaccgcc tccgccttcg gggtgcagag catccccacg gtcatcgcgt tcaaggacgg
    19681 tcaggcggtg acgggattca tcggagcgta tccggagccg gaggtgaacc gcttcatcga
    19741 ctcgatcctc ccgaccgagg ccgagaagca cgccgaggag gcgcgaacgg tggaggcgtc
    19801 cggggatctc gcgactgcgg agacgggcta ccggcaggtg ctcgaggagg acgagggcaa
    19861 tcgcgacgcc gcgatcgggc tggcccggat cctcgtggag cgtggcgatc tggacgaagc
    19921 ggagcaattc ctcggcaagc accggccgga ccctgaggcg gagaagctgc tcgcgtcgat
    19981 cgcggtgcgg cgatgggcca actcgcccgc ggacggcgtc ctcggcgagg cgcagcgcgt
    20041 cgtggcgtcg ggtgacctga gcggtggact ggccggtctg ctccaggcgc tgaaggagga
    20101 tcgcgaagcg gcccgcgacg cgatgatcac ggtcttcacg gcggtagggg acgaggaccc
    20161 cgtcgtgcag gagtaccgtc ggctgctttc cgccgcgctg ttctgacggc gtggacgttc
    20221 gcgaccagat ccgcgagtgg tgggacgacg acgccgatgc ctacgacgtc gcccccggtc
    20281 actcgctgtc cgacccggtg gaggcagctg cctggcgggc tgtgctcctc agcctccttc
    20341 cgcccgcgcc ggcgctcgtg ctggacgtcg gcgcggggac gggttcgatg tctctcctcg
    20401 cagcggagat gggtcacgtc gtgaccgccc tcgacctgtc gcaggggatg ctcgagcggg
    20461 cggaacggaa ggcctccgag cgcgggctcc ggatcgactt cgtggtgggt ccggccgagg
    20521 agccgccgct cgggtccttc gacgtcgtga cggcccgtca tctcttgtgg acgctgccgg
    20581 cgcccgccga ggcactcgcg gcatggagac gggcggtgcg cgacgacggt cgcctggtcc
    20641 tcttcgaggg ctcgtgggcg ggcgaagggc catggtcccg agcggcgggc gcggtcgcct
    20701 cctcggcgga gcggttgatg ggcgtccgcg aggaccatca cgctccgtac ccgcccgggg
    20761 tgatcgatcg gttgccgttg cagggcgcga gctccccggc cccgttcgtg gaggcggtcc
    20821 ggagtgccgg ctggtcccgg atccgcctcg tccggttgcg tgacgtcgag tggtcgatcg
    20881 agcgacgtgc accgtggccg ctcgggtggc tccgccggcg gccgcgctac gcgatcgtcg
    20941 ccgagtcata gggcccttcc cgccggaaga gcatctcggt taccggctcg cccgcgatct
    21001 cgaccccgat cgacggcgac gaacgaacca gcacttctcg aggacgcgga tcgatccggc
    21061 gttgtgctcg gcgacggtgc gcggagtgcg gccgttcctc gtccctctga tgtgcgaaga
    21121 agatcgggag gtcggcgccg gtgactccac gcagcgtgac acggcctgtc atccgtgtgt
    21181 ggagggtatc gggtcccgat cccatggcgt tccggtcgca tctcggtgac gaggctggcc
    21241 cccgggtgag ccggcgttcc atcatcggtg catggacgag acatgggtca cgctcggagt
    21301 gatgtgcatg gggctcggtg gggtcgtcgc ggggctcgtc ggagtggtgc tccccgaggg
    21361 ccggcggctc ggtgcgctgt tcgcgttgat cgctggtgca ggtgtggggc tggtggtcgt
    21421 cgggctgggt gctctgctgg acgagaggaa cgaaccgacg gagctgacgt tcttcctcgc
    21481 ctcgctcctc ggcctcctca cggtgtgtgg agcgtcgtgg gcggtgtggc ggagggccgc
    21541 gggttccggg ccttccacgg gagagccggc ctagaccgcg acgggacgga gcgcggccgc
    21601 gatcccgtcg ggagcgagga cgagctcgat gtcgaagacg tcggcgagat ggcgctcgag
    21661 cagcggcgcg acctccgcca ccgtcacggg gcgctccgcc agtcggctga gcgacgtcac
    21721 gccgtgtccc ggcaagccgc aggcgacgat cccatcgaac caggagaggt cggggtcgca
    21781 gttcaccgcg aagccgtgga cggtgacccg ggcgcggagc agccggacgc cgatcgcgca
    21841 gaccttgtcg tcgccgtgcc acacgccggt ctggacgtcc tcgcgccgct ggagggacac
    21901 gcccaggtcc tcgccggcgc ggatcacggc ctcctccatc cggcgcagat gcgccgccgc
    21961 atccggcttc ggcccgaggt cgaggatcgg gtagccgacg agctgtcccg gtccgtggaa
    22021 cgtgaaggcg ccgccgcggt cgatccgata gacctccgcg ccgcgcgccg cgagctcgcc
    22081 cggtgaccac aggagctcgt ccggccgcgc gcggcgcccg cccgtgaaga ccgggggatg
    22141 ttccagcagg atgaccacgt ccgggatctc gccggcgaga cggcgttccg cgagccggtg
    22201 catcgcgtcg ttcgcgaggc tgtaccgaac cggcccctcg ggccggacga gcagggcttc
    22261 ccgcctcatc gtgtggccgt caaacggcga cgtcctcggc gaagtcccaa ctctcgaggt
    22321 tctgcttcac ccgggcgagg aaccgggtgg cgatctcgcc gtcgatcgtc cggtgatccc
    22381 acgacatcga gatgttcacc atcgaacgga tcgcgatcgt gtcgtcgatc acgaccgctc
    22441 gcttctggat cgcgtcgaag gacaggatgc cggtgttggg ctggttgatg atcgggacac
    22501 tcgcgatcga cccgtacgga ccggggttgg tgatcgtgaa ggtcgatcct tggatctcgt
    22561 cgggcttgag ctggtgggca cgtgctcgac ccgcgaggtc agcgatcgcc cgggcgagac
    22621 ccacggtgtt catcgagtcc gctgccctga tcaccgggac gatgagtcct tcgtcgtagg
    22681 agacggcgat ccccatgtgg accgcgcgat gccgcacgat ctcgtcgccc cggagcgagg
    22741 agttgacctc cgggtgggtc atcagggcat cgcacgtcgc gcggatcacg aaggggaggt
    22801 acgtcaggtt gacaccgtgc ttcaccttga aagattcctt cgcccgttcg cggagcttca
    22861 cgaggtggtc gacgttcacc tcgaccatcg tccacgcacg cgccgaggtc tggagggagg
    22921 ccagcatgtg ctggccgatc agtttgcgga tgtgcgagac gggggcgacc tcctcgtcgg
    22981 gccccgcggc cgcctggggg gcgggagaag tcgcagctgc cggtgccggc gcgggtgccg
    23041 cggccggcac ctcggcggtc gcgggtgccg ctgcggcgcc atcgccgtcg atcgccgcga
    23101 ggacgtcctt cttcgtgatg cgtccccccg tcccggaccc ctcgacgcgc gagaggtcga
    23161 ggccgtgctc gtcggcgagc cgccgcacga gcggtgaaag gatccgcgac cgcggccctc
    23221 ggtcgggaag gtctccggat ccgccgtccg ggggggcggg atcggccgcc tgccccggtg
    23281 cggtcccgct ggccgccgtg gcctcggcac cttgaacggt cgccggctcc acggctggca
    23341 tctcggcggc cgagccctcg gcgaccgcct cggcgctctc cgacggcgct tccttcactg
    23401 tctcaggcac tgtctcaggc gccgtctcag gcgctgggtc ccgctgcgac tcgttctgcg
    23461 acggcgcccc ggcgtcggat gaggcttggc cgccggaggc cgcccctccg ccgctgccgt
    23521 cctcgctcac ctcggcgaga tccgtgccca ccgggaccgt ctgcccctcc tgcaccagga
    23581 tcttcgtgac ggtgccgccc accgacgagg ggacctccgt atcgaccttg tcggtcgaga
    23641 tctcgaagag gggttcatcg cgttcgatgg tctccccttc ctgcttcagc cacttcagga
    23701 tcgttccctc gacgatggtc tcgccgagct ggggcatctt gatcgtggtc atcgggctct
    23761 cctcacgctc accaggccga gagcttcttc accgtcgcga cgatgtcgtc gacccccggc
    23821 aggaaggcat cctccaacgt gggggagaag gggatcggga cgttgggcgg cgcgagccgc
    23881 acgacggggg cgtcgaggtg ctcgaacgct ttctccgcga tcgtggacgc gacctccgct
    23941 gcgatcccga cgaaccggta ggactcggag agcacgaccg ccctcgccgt cttctcgatc
    24001 gaccgcagca ccgtctcctc gtccatcggg acgatcgtgc ggagatcgac gacctccgcg
    24061 gagacccctt ctgcctcgag cgtctgcgcc gcctcgaggg cacggtggac catcgcgccg
    24121 tacgtgatga tcgagacgtc gtcgccctcg cgcttcacgt tggcggagcc gatcgggacg
    24181 aggaagtccg gatcctccga cacctgctcg cggacgcgcc ggtagatcca cttgtgttcg
    24241 aggtacatca ccgggttgtc gtcgcggatc gccgacttca acaagccctt cgcgtccgcg
    24301 gggaaggcgg gacacacgat cttgatcccg cccgagtacg cgaaccaggg ctccgggttg
    24361 atcgagtgga aggacgaggc gcggaccatc ccgcccgagg gccctcggag caccaccggc
    24421 agtggaacgc cgctccggta gtggacgcgg gcgagcgccg tggagatctc gtcgaagccg
    24481 ctggtcatga agtcggcgta ctggaactcc gcaacggggc ggaacccctc catcgcggag
    24541 ccgatcgcca tgccgacgat cgtctcctcg gcgatcggcg tgtccatcac ccggtccgcg
    24601 ccgaaccggt cgtagaagcc ttcggtgacc ttgaacgcac cgccgtacac gccgatgtcc
    24661 tcgccgagca tgtagacgcg ctcgtcgcgc tccatctcct cccagagcgc ctccgcgatc
    24721 gcaacgaggt acgtggcttg accgtcctcg gtgcgcttgc ctcgaggctc cgccccgtgg
    24781 ccggccgcgc cggtctcgac ctcgcggttc ttcggccggg tgtcctgcgc ttgtgccatc
    24841 aggcggcctc cgccatcatg cggcctccgc catcacgagg cctctgtccc gccgccgcgg
    24901 ccgggctcac tcgtccagta gccgtcctcg acccacagac ccttcgagaa gtcttcgggc
    24961 tcggggaacg gcgatgactg cgcgaactcg tagccctcct cgaactcctt ctcgatccgc
    25021 ccctggatat cggcgacctc ctggtcgtcg acgacaccgt ccgacaggag tcgttgttcc
    25081 aagatcttga cgggatcctt gtcgcgcatg tactcctcga ggagctcctt ggggacgtac
    25141 ctggccgggt cgtgtcccgc gtggcccttc cagcggaggg aaaccgattc gatcaggctc
    25201 gggccgccgc cgccgcgcgc acgctcgacc gcctctcgtg ccgcagcctc caccgcgagc
    25261 acatccgcac cgtcgacccg cacgcccggc atcccgtacg ccgccgcgcg ctccgcgatc
    25321 gtagggacgg agaactcgag ctcgttcggc gcggagtacg cgtagaggtt gttgttcacg
    25381 acccacacca cggggagctg cgcgatggcc gacatgttga gcgactcgtg ccagcgcccg
    25441 ttcgacgtcg atccgtcgcc gcagatcgcc atcgcgacgt ggggctcacc ccgccgcttg
    25501 taggcgagcg cggccccgca cgccacgggg taggcgatcg ggagatgcga catcacggtc
    25561 cacgtcttgc tcccgtacca gtccccgatg tggctattcc cgtcgcgacc tcgtgtgtac
    25621 ccgtcgatcc ggccccagta ctgcgccatg atgcgcttca ggtcgaggcc tttcgcgatc
    25681 tgcgcggaca ggtcgcgatg cgtggggaac agcgagtcct cgggcccgag cgggaggacg
    25741 acaccgatgt gcgtcccttc ctgcccccgg ccggagtaga tcgctccggg tagcctgccc
    25801 tggcggtaca gggattccat ccgctcgtcg aagtagcgac agagcttcag gaggtacagg
    25861 gcccgcttca ggtcgtcgtc cgagagtcgc ggatcggtgt ccttccgctg acgggtctgg
    25921 gtcgtcgcca cgaggctcct caggccgtgt ggagcggctt gccggcgagc gccagatggg
    25981 cttcgccgat cgcttcggac agggacgggt gcggatggat cagcgcggcg acctccgcgg
    26041 ggaccgcctc ccagttcgtg atcagcatcg cctccgcgat cagatcggtg acgtgggggc
    26101 cgatcatgtg gacccccagg acgggcccgc cgccctgctc gctcacgacc ttcacgaagc
    26161 ccccctcgcc gagcatgttc gccttgccga tcccccggaa gtcgagatgc gcggtcgcga
    26221 ccttgcgccc ggcgtccttc gcctgtccgg ccgtcaagcc gacgctcgcg atctcgggcg
    26281 tgcagtacgt gacgcgcggg atgttgacgt agtcgatgtc cggcacggtc tcgccggcga
    26341 tgcgctcggc cacggcgtag ccctccgtga acgccacgtg cgccagctgg agcggggtgt
    26401 tggccacgtc accgacggcc cagacgtgct cgacgttcgt ccgcagctga ccgtccacgt
    26461 cgacgaagcc cttctcgtgc acagcgaccc cggcgtcctc gagcccgagg ccctcggtca
    26521 ccggtccgcg accgatcgcc acgaggcaga tgtcggcggt caccctctcg gccttcccgc
    26581 cggcttcgaa cgtgacgtcg acggactcac ccgtgtcggc gatctccttc acggatgcgc
    26641 cggacgacgc cttgatgcca cgacgccgga acgccttggc gacctccgcg gagacgtcct
    26701 cgtcctcgag cggcgccagg ccgggcagag cctccaggag cgtgacctcc gcgcccagcg
    26761 atcgatagag agaggcgaac tccagtccca cggcgcccgc cccgatcacg acggcggagc
    26821 cggggatccg gtcgtaccgg agcgcctcgt cgctcgtgat gacgcgatcg gtgacctcca
    26881 caccggggag cagtcgtggc gccgaaccgg tggcgatcac gacgtcggtg gcgtcgaccc
    26941 gctgcccctc gacctcgagc gccggccccg ggagcagctt cgccgtcccc ttcagaacgg
    27001 tgatgccgcg ggtcttgatc agtcccgtga cgcctttgac caacttgtcc acgatcttgt
    27061 cctcgaaggc tcggaccgcg gaccaatcgg ggtcacccga cgccttgatg ccccactcct
    27121 gtgcgcggtt cacggtgtcc atcacggcag cggactggag cagtgccttc gtcgggatgc
    27181 agccgcggag gagacaggtg ccacccagtc ggtcgtggcg ctcgacgagg gcaacgcgtt
    27241 tgcccagctg tgccgcccgc agggccgttg aatagccacc ggtgccggcg ccgaccacaa
    27301 gtacgtcgta cgcgtccgcc atcgagtgtc gagtctactc ggcatcctct ggacggcatc
    27361 ccgcagcacc tgtgcggccg atacgatgcc gtcccgatgg cgcacgcgag gcccgccgac
    27421 ccggccgatg aagccgaggc cgtctccgac gagctcaaac ggaccccgtt ggaggccgag
    27481 catcttcgcc tcggcgcgaa gatggcccct ttcgcgggat ggcagatgcc gatcgagtac
    27541 gagggcgcgc tcgcggagca tcgggccgtg cgatcgcatg tcggattgtt cgacctcacg
    27601 catctcggca aggtggaggt ggtgggtgcc ggcgcgttgg gcatgctcca acggctggtg
    27661 acgaacgacc tctcccgcgc ccgcgtgggc gaggcgctct acaacctcgt gttgaacgag
    27721 ggcggaggcg tgatcgagga cctgatcgtc tatcgcctcg gcgaggagcg ctacttcgtg
    27781 gtgccgaacg cctccaacgc gccccgggtg ctccggatgc tcgaagaaga gaagtccgac
    27841 gagccactcc acctgatgta ccaccaggac tggtgcttcc tcgcggtcca ggggcccgaa
    27901 gcgatgagcg cgatcgccgc gacgttcccc acggctcccg aactcgcctt catgcgatgc
    27961 atggagaccg agtaccaccg ccggccggtg atcgtgacgc ggtccggcta caccggggag
    28021 gtggggttcg agctgttcac ctaccaggac gtcgcccagc agctctggga cgagctgctc
    28081 ggctcggtcc gcccgctcgg aggcgctccc tgcgggctcg cctcccgcga catcctccgg
    28141 ctcgagatgg gatacgcgct ctacggccag gacctcttcg aggcatcgac ggcgttcgag
    28201 gcggg
//
